LOCUS       DAJVXP010000001        88080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVXP010000001 DAJVXP010000000
VERSION     DAJVXP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.001"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OQS10_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OQS10_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS2291148.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OQS10_000002"
     tRNA            1230..1305
                     /locus_tag="OQS10_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OQS10_000003"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="OQS10_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OQS10_000004"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="OQS10_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS2291149.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OQS10_000005"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="OQS10_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS2291150.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OQS10_000006"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="OQS10_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS2291151.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="OQS10_000007"
     CDS             complement(4869..5306)
                     /locus_tag="OQS10_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS2291152.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="OQS10_000008"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="OQS10_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OQS10_000009"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="OQS10_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCS2291153.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="OQS10_000010"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="OQS10_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCS2291154.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="OQS10_000011"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="OQS10_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCS2291155.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="OQS10_000012"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="OQS10_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCS2291156.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="OQS10_000013"
     CDS             10774..11442
                     /locus_tag="OQS10_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCS2291157.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="OQS10_000014"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="OQS10_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCS2291158.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="OQS10_000015"
     CDS             11985..13817
                     /locus_tag="OQS10_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCS2291159.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="OQS10_000016"
     CDS             13807..14157
                     /locus_tag="OQS10_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCS2291160.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="OQS10_000017"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="OQS10_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCS2291161.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="OQS10_000018"
     CDS             14476..14898
                     /locus_tag="OQS10_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCS2291162.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="OQS10_000019"
     CDS             14901..15533
                     /locus_tag="OQS10_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCS2291163.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="OQS10_000020"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="OQS10_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCS2291164.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="OQS10_000021"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="OQS10_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCS2291165.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="OQS10_000022"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="OQS10_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCS2291166.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="OQS10_000023"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="OQS10_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="OQS10_000024"
     CDS             18712..19824
                     /locus_tag="OQS10_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCS2291167.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="OQS10_000025"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="OQS10_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="OQS10_000026"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="OQS10_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCS2291168.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="OQS10_000027"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="OQS10_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCS2291169.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /locus_tag="OQS10_000028"
     CDS             22078..22521
                     /locus_tag="OQS10_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCS2291170.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="OQS10_000029"
     CDS             complement(22622..22951)
                     /locus_tag="OQS10_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS2291171.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="OQS10_000030"
     CDS             complement(23123..24181)
                     /locus_tag="OQS10_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS2291172.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="OQS10_000031"
     CDS             complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="OQS10_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCS2291173.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OQS10_000032"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="OQS10_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCS2291174.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="OQS10_000033"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="OQS10_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS2291175.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /locus_tag="OQS10_000034"
     CDS             complement(27816..28043)
                     /locus_tag="OQS10_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS2291176.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OQS10_000035"
     CDS             complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="OQS10_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS2291177.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OQS10_000036"
     CDS             complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="OQS10_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS2291178.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OQS10_000037"
     CDS             complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="OQS10_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS2291179.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30919..31848)
                     /locus_tag="OQS10_000038"
     CDS             complement(30919..31848)
                     /locus_tag="OQS10_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2291180.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31894..32718)
                     /locus_tag="OQS10_000039"
     CDS             complement(31894..32718)
                     /locus_tag="OQS10_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2291181.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OQS10_000040"
     CDS             complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="OQS10_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS2291182.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OQS10_000041"
     CDS             complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="OQS10_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS2291183.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33586..33636
                     /gene="hisL"
                     /locus_tag="OQS10_000042"
     CDS             33586..33636
                     /gene="hisL"
                     /locus_tag="OQS10_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS2291184.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33612..33735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33782..34681
                     /gene="hisG"
                     /locus_tag="OQS10_000043"
     CDS             33782..34681
                     /gene="hisG"
                     /locus_tag="OQS10_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS2291185.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34687..35991
                     /gene="hisD"
                     /locus_tag="OQS10_000044"
     CDS             34687..35991
                     /gene="hisD"
                     /locus_tag="OQS10_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS2291186.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35988..37058
                     /gene="hisC"
                     /locus_tag="OQS10_000045"
     CDS             35988..37058
                     /gene="hisC"
                     /locus_tag="OQS10_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS2291187.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37058..38125
                     /gene="hisB"
                     /locus_tag="OQS10_000046"
     CDS             37058..38125
                     /gene="hisB"
                     /locus_tag="OQS10_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS2291188.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38125..38715
                     /gene="hisH"
                     /locus_tag="OQS10_000047"
     CDS             38125..38715
                     /gene="hisH"
                     /locus_tag="OQS10_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS2291189.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38715..39452
                     /gene="hisA"
                     /locus_tag="OQS10_000048"
     CDS             38715..39452
                     /gene="hisA"
                     /locus_tag="OQS10_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS2291190.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39434..40210
                     /gene="hisF"
                     /locus_tag="OQS10_000049"
     CDS             39434..40210
                     /gene="hisF"
                     /locus_tag="OQS10_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS2291191.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40204..40815
                     /locus_tag="OQS10_000050"
     CDS             40204..40815
                     /locus_tag="OQS10_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS2291192.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OQS10_000051"
     CDS             complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="OQS10_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS2291193.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OQS10_000052"
     CDS             complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="OQS10_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS2291194.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OQS10_000053"
     CDS             complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="OQS10_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS2291195.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OQS10_000054"
                     /gene_synonym="manB"
     CDS             complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="OQS10_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS2291196.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OQS10_000055"
     CDS             complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="OQS10_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS2291197.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OQS10_000056"
     CDS             complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="OQS10_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS2291198.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OQS10_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="OQS10_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS2291199.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OQS10_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="OQS10_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS2291200.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OQS10_000059"
     CDS             complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="OQS10_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS2291201.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OQS10_000060"
     CDS             complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="OQS10_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS2291202.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OQS10_000061"
     CDS             complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="OQS10_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS2291203.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OQS10_000062"
     CDS             complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="OQS10_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS2291204.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OQS10_000063"
     CDS             complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="OQS10_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS2291205.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OQS10_000064"
     CDS             complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="OQS10_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS2291206.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OQS10_000065"
     CDS             complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="OQS10_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS2291207.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OQS10_000066"
     CDS             complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="OQS10_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS2291208.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OQS10_000067"
     CDS             complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="OQS10_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS2291209.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OQS10_000068"
     CDS             complement(59516..60655)
                     /gene="wza"
                     /locus_tag="OQS10_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS2291210.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60964..61113
                     /locus_tag="OQS10_000069"
     CDS             60964..61113
                     /locus_tag="OQS10_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291211.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61314..62897
                     /locus_tag="OQS10_000070"
     CDS             61314..62897
                     /locus_tag="OQS10_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS2291212.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63171..65024)
                     /gene="asmA"
                     /locus_tag="OQS10_000071"
     CDS             complement(63171..65024)
                     /gene="asmA"
                     /locus_tag="OQS10_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS2291213.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OQS10_000072"
     CDS             complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="OQS10_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS2291214.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OQS10_000073"
     CDS             complement(65719..66360)
                     /gene="udk"
                     /locus_tag="OQS10_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS2291215.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66678..69995
                     /locus_tag="OQS10_000074"
     CDS             66678..69995
                     /locus_tag="OQS10_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS2291216.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OQS10_000075"
     CDS             complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="OQS10_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS2291217.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71087..72439
                     /gene="yegD"
                     /locus_tag="OQS10_000076"
     CDS             71087..72439
                     /gene="yegD"
                     /locus_tag="OQS10_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2291218.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OQS10_000077"
     CDS             complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="OQS10_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS2291219.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OQS10_000078"
     CDS             complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="OQS10_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS2291220.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75147..75806)
                     /locus_tag="OQS10_000079"
     CDS             complement(75147..75806)
                     /locus_tag="OQS10_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS2291221.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OQS10_000080"
     CDS             complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="OQS10_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS2291222.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76359..76415)
                     /locus_tag="OQS10_000081"
     CDS             complement(76359..76415)
                     /locus_tag="OQS10_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2291223.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76686..76742)
                     /locus_tag="OQS10_000082"
     CDS             complement(76686..76742)
                     /locus_tag="OQS10_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2291224.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77021..78262
                     /gene="mdtA"
                     /locus_tag="OQS10_000083"
     CDS             77021..78262
                     /gene="mdtA"
                     /locus_tag="OQS10_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS2291225.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78262..81384
                     /gene="mdtB"
                     /locus_tag="OQS10_000084"
     CDS             78262..81384
                     /gene="mdtB"
                     /locus_tag="OQS10_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS2291226.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81385..84462
                     /gene="mdtC"
                     /locus_tag="OQS10_000085"
     CDS             81385..84462
                     /gene="mdtC"
                     /locus_tag="OQS10_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS2291227.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84463..85878
                     /locus_tag="OQS10_000086"
     CDS             84463..85878
                     /locus_tag="OQS10_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2291228.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85875..87278
                     /gene="baeS"
                     /locus_tag="OQS10_000087"
     CDS             85875..87278
                     /gene="baeS"
                     /locus_tag="OQS10_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS2291229.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87275..87997
                     /gene="baeR"
                     /locus_tag="OQS10_000088"
     CDS             87275..87997
                     /gene="baeR"
                     /locus_tag="OQS10_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS2291230.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21562 a        23316 c        22148 g        21054 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatc gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctgaaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    29221 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    29281 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    29341 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    29401 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    29461 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    29521 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    29581 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    29641 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    29701 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    29761 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    29821 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    29881 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    29941 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    30001 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    30061 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    30121 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    30181 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    30241 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    30301 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    30361 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    30421 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    30481 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    30541 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    30601 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    30661 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    30721 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    30781 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    30841 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    30901 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    30961 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    31021 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    31081 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    31141 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    31201 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    31261 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    31321 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    31381 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    31441 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    31501 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    31561 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    31621 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    31681 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    31741 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    31801 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    31861 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    31921 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    31981 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    32041 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    32101 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    32161 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    32221 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    32281 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    32341 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    32401 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    32461 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    32521 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    32581 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    32641 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    32701 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    32761 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    32821 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    32881 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    32941 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    33001 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    33061 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    33121 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    33181 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    33241 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    33301 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    33361 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    33421 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    33481 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    33541 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    33601 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    33661 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    33721 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    33781 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    33841 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    33901 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    33961 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    34021 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    34081 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    34141 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    34201 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    34261 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    34321 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    34381 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    34441 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    34501 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    34561 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    34621 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    34681 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    34741 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    34801 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    34861 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    34921 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    34981 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    35041 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    35101 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    35161 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    35221 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    35281 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    35341 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    35401 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    35461 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    35521 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    35581 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    35641 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    35701 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    35761 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    35821 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    35881 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    35941 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    36001 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    36061 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    36121 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    36181 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    36241 acgaaggtat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct
    36301 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    36361 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    36421 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    36481 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    36541 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    36601 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    36661 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    36721 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    36781 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    36841 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    36901 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    36961 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    37021 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    37081 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    37141 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    37201 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    37261 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    37321 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    37381 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    37441 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    37501 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    37561 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    37621 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    37681 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    37741 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    37801 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    37861 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    37921 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    37981 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    38041 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    38101 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    38161 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    38221 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    38281 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    38341 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    38401 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    38461 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    38521 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    38581 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    38641 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    38701 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    38761 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    38821 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    38881 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    38941 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    39001 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    39061 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    39121 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    39181 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    39241 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    39301 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    39361 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    39421 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    39481 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    39541 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    39601 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    39661 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    39721 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    39781 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    39841 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    39901 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    39961 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    40021 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    40081 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    40141 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    40201 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    40261 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    40321 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    40381 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    40441 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    40501 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    40561 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    40621 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    40681 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    40741 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    40801 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    40861 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    40921 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    40981 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    41041 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    41101 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    41161 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    41221 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    41281 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    41341 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    41401 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    41461 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    41521 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    41581 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    41641 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    41701 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    41761 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcgctag
    41821 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    41881 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    41941 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    42001 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    42061 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    42121 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    42181 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    42241 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    42301 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    42361 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    42421 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    42481 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    42541 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    42601 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    42661 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    42721 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    42781 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    42841 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    42901 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    42961 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    43021 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    43081 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    43141 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    43201 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    43261 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    43321 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    43381 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    43441 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    43501 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    43561 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    43621 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    43681 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    43741 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    43801 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    43861 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    43921 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    43981 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    44041 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    44101 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    44161 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    44221 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    44281 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    44341 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    44401 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    44461 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    44521 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    44581 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    44641 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    44701 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    44761 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    44821 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    44881 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    44941 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    45001 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    45061 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    45121 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    45181 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    45241 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    45301 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    45361 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    45421 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    45481 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    45541 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    45601 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    45661 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    45721 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    45781 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    45841 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    45901 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    45961 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    46021 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    46081 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    46141 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    46201 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    46261 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    46321 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    46381 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    46441 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    46501 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    46561 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    46621 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    46681 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    46741 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    46801 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    46861 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    46921 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    46981 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    47041 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    47101 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    47161 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    47221 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    47281 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    47341 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    47401 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    47461 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    47521 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    47581 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    47641 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    47701 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    47761 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    47821 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    47881 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    47941 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    48001 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    48061 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    48121 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    48181 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    48241 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    48301 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    48361 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    48421 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    48481 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    48541 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    48601 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    48661 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    48721 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    48781 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    48841 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    48901 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    48961 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    49021 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    49081 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    49141 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    49201 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    49261 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    49321 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    49381 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    49441 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    49501 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    49561 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    49621 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    49681 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    49741 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    49801 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    49861 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    49921 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    49981 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    50041 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    50101 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    50161 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    50221 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    50281 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    50341 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    50401 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    50461 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    50521 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    50581 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    50641 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    50701 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    50761 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    50821 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    50881 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    50941 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    51001 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    51061 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    51121 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    51181 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    51241 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    51301 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    51361 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    51421 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    51481 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    51541 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    51601 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    51661 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    51721 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    51781 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    51841 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    51901 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    51961 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    52021 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    52081 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    52141 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    52201 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    52261 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    52321 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    52381 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    52441 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    52501 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    52561 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    52621 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    52681 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    52741 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    52801 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    52861 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    52921 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    52981 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    53041 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    53101 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    53161 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    53221 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    53281 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    53341 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    53401 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    53461 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    53521 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    53581 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    53641 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    53701 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    53761 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    53821 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    53881 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    53941 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    54001 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    54061 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    54121 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    54181 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    54241 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    54301 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    54361 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    54421 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    54481 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    54541 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    54601 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    54661 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    54721 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    54781 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    54841 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    54901 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    54961 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    55021 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    55081 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    55141 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    55201 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    55261 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    55321 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    55381 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    55441 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    55501 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    55561 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    55621 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    55681 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    55741 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    55801 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    55861 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    55921 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    55981 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    56041 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    56101 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    56161 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    56221 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    56281 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    56341 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    56401 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    56461 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    56521 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    56581 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    56641 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    56701 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    56761 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    56821 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    56881 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    56941 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    57001 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    57061 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    57121 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    57181 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    57241 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    57301 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    57361 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    57421 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    57481 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    57541 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    57601 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    57661 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    57721 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    57781 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    57841 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    57901 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    57961 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    58021 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    58081 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    58141 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    58201 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    58261 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    58321 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    58381 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    58441 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    58501 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    58561 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    58621 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    58681 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    58741 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    58801 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    58861 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    58921 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    58981 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    59041 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    59101 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    59161 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    59221 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    59281 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    59341 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    59401 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    59461 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    59521 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    59581 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    59641 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    59701 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    59761 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    59821 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    59881 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    59941 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    60001 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    60061 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    60121 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    60181 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    60241 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    60301 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    60361 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    60421 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    60481 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    60541 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    60601 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    60661 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    60721 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    60781 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    60841 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    60901 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    60961 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    61021 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    61081 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    61141 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    61201 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    61261 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    61321 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    61381 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    61441 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    61501 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    61561 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    61621 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    61681 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    61741 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    61801 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    61861 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    61921 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    61981 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    62041 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    62101 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    62161 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    62221 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    62281 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    62341 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    62401 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    62461 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    62521 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    62581 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    62641 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    62701 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    62761 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    62821 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    62881 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    62941 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    63001 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    63061 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    63121 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac ctacatcttc
    63181 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    63241 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    63301 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    63361 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    63421 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    63481 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    63541 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    63601 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    63661 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    63721 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    63781 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    63841 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    63901 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    63961 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    64021 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    64081 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    64141 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    64201 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    64261 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    64321 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    64381 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    64441 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    64501 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    64561 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    64621 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    64681 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    64741 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    64801 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    64861 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    64921 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    64981 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    65041 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    65101 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    65161 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    65221 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    65281 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    65341 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    65401 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    65461 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    65521 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    65581 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    65641 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    65701 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    65761 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    65821 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    65881 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    65941 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    66001 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    66061 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    66121 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    66181 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    66241 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    66301 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    66361 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    66421 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    66481 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    66541 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    66601 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    66661 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    66721 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    66781 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    66841 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    66901 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    66961 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    67021 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    67081 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    67141 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    67201 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    67261 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    67321 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    67381 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    67441 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    67501 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    67561 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    67621 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    67681 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    67741 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    67801 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    67861 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    67921 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    67981 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    68041 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    68101 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    68161 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    68221 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    68281 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    68341 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    68401 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    68461 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    68521 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    68581 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    68641 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    68701 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    68761 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    68821 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    68881 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    68941 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    69001 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    69061 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    69121 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    69181 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    69241 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    69301 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    69361 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    69421 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    69481 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    69541 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    69601 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    69661 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    69721 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    69781 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    69841 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    69901 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    69961 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    70021 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    70081 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    70141 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    70201 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    70261 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    70321 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    70381 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    70441 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    70501 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    70561 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    70621 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    70681 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    70741 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    70801 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    70861 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    70921 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    70981 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    71041 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    71101 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    71161 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    71221 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    71281 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    71341 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    71401 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    71461 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    71521 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    71581 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    71641 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    71701 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    71761 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    71821 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    71881 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    71941 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    72001 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    72061 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    72121 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    72181 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    72241 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    72301 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    72361 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    72421 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    72481 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    72541 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    72601 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    72661 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    72721 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    72781 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    72841 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    72901 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    72961 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    73021 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    73081 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    73141 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    73201 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    73261 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    73321 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    73381 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    73441 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    73501 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    73561 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    73621 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    73681 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    73741 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    73801 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    73861 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    73921 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    73981 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    74041 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    74101 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    74161 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    74221 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    74281 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    74341 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    74401 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    74461 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    74521 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    74581 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    74641 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    74701 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    74761 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    74821 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    74881 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    74941 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    75001 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    75061 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    75121 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    75181 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    75241 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    75301 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    75361 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    75421 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    75481 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    75541 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    75601 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    75661 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    75721 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    75781 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    75841 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    75901 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    75961 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    76021 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    76081 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    76141 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    76201 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    76261 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    76321 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    76381 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    76441 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    76501 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    76561 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    76621 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    76681 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    76741 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    76801 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    76861 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    76921 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    76981 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    77041 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    77101 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    77161 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    77221 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    77281 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    77341 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    77401 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    77461 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    77521 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    77581 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    77641 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    77701 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    77761 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    77821 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    77881 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    77941 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    78001 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    78061 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    78121 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    78181 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    78241 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    78301 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    78361 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    78421 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    78481 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    78541 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    78601 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    78661 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    78721 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    78781 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    78841 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    78901 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    78961 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    79021 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    79081 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    79141 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    79201 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    79261 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    79321 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    79381 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    79441 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    79501 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    79561 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    79621 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    79681 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    79741 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    79801 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    79861 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    79921 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    79981 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    80041 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    80101 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    80161 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    80221 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    80281 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    80341 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    80401 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    80461 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    80521 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    80581 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    80641 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    80701 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    80761 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    80821 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    80881 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    80941 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    81001 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    81061 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    81121 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    81181 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    81241 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    81301 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    81361 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    81421 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    81481 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    81541 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    81601 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    81661 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    81721 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    81781 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    81841 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    81901 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    81961 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    82021 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    82081 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    82141 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    82201 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    82261 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    82321 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    82381 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    82441 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    82501 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    82561 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    82621 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    82681 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    82741 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    82801 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    82861 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    82921 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    82981 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    83041 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    83101 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    83161 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    83221 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    83281 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    83341 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    83401 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    83461 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    83521 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    83581 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    83641 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    83701 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    83761 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    83821 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    83881 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    83941 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    84001 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    84061 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    84121 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    84181 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    84241 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    84301 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    84361 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    84421 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    84481 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    84541 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    84601 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    84661 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    84721 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    84781 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    84841 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    84901 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    84961 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    85021 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    85081 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    85141 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    85201 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    85261 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    85321 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    85381 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    85441 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    85501 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    85561 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    85621 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    85681 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    85741 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    85801 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    85861 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    85921 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    85981 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    86041 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    86101 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    86161 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    86221 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    86281 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    86341 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    86401 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    86461 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    86521 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    86581 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    86641 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    86701 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    86761 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    86821 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    86881 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    86941 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    87001 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    87061 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    87121 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    87181 cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    87241 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    87301 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    87361 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    87421 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    87481 cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc
    87541 aaaaatcgaa gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg
    87601 caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    87661 accgcagcgc gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    87721 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt
    87781 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    87841 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    87901 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    87961 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag
    88021 ccggagatgg cgttcagacc agatccggca acattatccc acgcatggtc agcaaactga
//
LOCUS       DAJVXP010000002        86618 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVXP010000002 DAJVXP010000000
VERSION     DAJVXP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.002"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            37..1191
                     /locus_tag="OQS10_000089"
     CDS             37..1191
                     /locus_tag="OQS10_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS2291231.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            1342..2166
                     /locus_tag="OQS10_000090"
     CDS             1342..2166
                     /locus_tag="OQS10_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCS2291232.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINNSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
     gene            2252..3454
                     /locus_tag="OQS10_000091"
     CDS             2252..3454
                     /locus_tag="OQS10_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCS2291233.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPNDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            3514..4098
                     /locus_tag="OQS10_000092"
     CDS             3514..4098
                     /locus_tag="OQS10_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS2291234.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKDPVVMDSLSDCNNDLYKTLESIVRESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            4493..4951
                     /gene="traH"
                     /locus_tag="OQS10_000093"
     CDS             4493..4951
                     /gene="traH"
                     /locus_tag="OQS10_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HCS2291235.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            4948..5766
                     /gene="traI"
                     /locus_tag="OQS10_000094"
     CDS             4948..5766
                     /gene="traI"
                     /locus_tag="OQS10_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HCS2291236.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            5763..6911
                     /gene="traJ"
                     /locus_tag="OQS10_000095"
     CDS             5763..6911
                     /gene="traJ"
                     /locus_tag="OQS10_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCS2291237.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVVPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAVSITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            6908..7198
                     /locus_tag="OQS10_000096"
     CDS             6908..7198
                     /locus_tag="OQS10_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCS2291238.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            7213..7764
                     /locus_tag="OQS10_000097"
     CDS             7213..7764
                     /locus_tag="OQS10_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HCS2291239.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            7854..11621
                     /locus_tag="OQS10_000098"
     CDS             7854..11621
                     /locus_tag="OQS10_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS2291240.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESLASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATTASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTDTVE
                     PAVRDDPTPQQPVQSTSSSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PAPDSIVYAPERPDAPISLNLDEIIKSLEGEERADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATDFYGGVIELTGSDAFKQKAMQLIIEHNIKVRMKFPDQRAA
                     LEKLRKEMAVGKDTVVTHKPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            11639..11986
                     /locus_tag="OQS10_000099"
     CDS             11639..11986
                     /locus_tag="OQS10_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCS2291241.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            11983..12675
                     /locus_tag="OQS10_000100"
     CDS             11983..12675
                     /locus_tag="OQS10_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCS2291242.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            12686..13669
                     /gene="traN"
                     /locus_tag="OQS10_000101"
     CDS             12686..13669
                     /gene="traN"
                     /locus_tag="OQS10_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HCS2291243.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNT
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            13672..14961
                     /gene="traO"
                     /locus_tag="OQS10_000102"
     CDS             13672..14961
                     /gene="traO"
                     /locus_tag="OQS10_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCS2291244.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            14961..15665
                     /gene="traP"
                     /locus_tag="OQS10_000103"
     CDS             14961..15665
                     /gene="traP"
                     /locus_tag="OQS10_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HCS2291245.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            15665..16192
                     /gene="traQ"
                     /locus_tag="OQS10_000104"
     CDS             15665..16192
                     /gene="traQ"
                     /locus_tag="OQS10_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCS2291246.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            16243..16647
                     /gene="traR"
                     /locus_tag="OQS10_000105"
     CDS             16243..16647
                     /gene="traR"
                     /locus_tag="OQS10_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HCS2291247.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLASC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            16711..16899
                     /gene="traS"
                     /locus_tag="OQS10_000106"
     CDS             16711..16899
                     /gene="traS"
                     /locus_tag="OQS10_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HCS2291248.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            16883..17683
                     /gene="traT"
                     /locus_tag="OQS10_000107"
     CDS             16883..17683
                     /gene="traT"
                     /locus_tag="OQS10_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HCS2291249.1"
                     /translation="MQHSIKNLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLVKLIRRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTEPLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            17773..20817
                     /gene="traU"
                     /locus_tag="OQS10_000108"
     CDS             17773..20817
                     /gene="traU"
                     /locus_tag="OQS10_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCS2291250.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWALSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            20817..21431
                     /gene="traV"
                     /locus_tag="OQS10_000109"
     CDS             20817..21431
                     /gene="traV"
                     /locus_tag="OQS10_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HCS2291251.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            21398..22600
                     /gene="traW"
                     /locus_tag="OQS10_000110"
     CDS             21398..22600
                     /gene="traW"
                     /locus_tag="OQS10_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HCS2291252.1"
                     /translation="MQRKTLLAALIAILNGTAYQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            22629..23213
                     /gene="traX"
                     /locus_tag="OQS10_000111"
     CDS             22629..23213
                     /gene="traX"
                     /locus_tag="OQS10_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HCS2291253.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNSRNGWRNATLIAVTSKQY"
     gene            23310..25478
                     /locus_tag="OQS10_000112"
     CDS             23310..25478
                     /locus_tag="OQS10_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCS2291254.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYR
                     TQLQNSSYTPPLGTVVSSSEQRMANAQDPKSALSEIMGPLVSLTNRIATETSGTGTTS
                     AQINPLIKMKNIGDITMVSAEGIWTIYTTARVVVAGGKDSILVKFFNSLTGAASMLTA
                     LFEALAPPVYFLLFLMFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGRDGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDNTKGSDDKDGIKG"
     gene            25549..26211
                     /gene="excA"
                     /locus_tag="OQS10_000113"
     CDS             25549..26211
                     /gene="excA"
                     /locus_tag="OQS10_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCS2291255.1"
                     /translation="MKAKRETTDRFPTWWLFYYVLRKAYFFLGIPFFLFCALGFTEML
                     CSDRYFGNKVEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGVPVAE
                     VF"
     gene            complement(26283..26492)
                     /locus_tag="OQS10_000114"
     CDS             complement(26283..26492)
                     /locus_tag="OQS10_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCS2291256.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDITLAAIYALGEGRCQADNIIRE
                     LERLSQSDDMEIKIAAIKALGRIYR"
     gene            26824..27000
                     /locus_tag="OQS10_000115"
     CDS             26824..27000
                     /locus_tag="OQS10_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291257.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVSGKTRLMFDSK
                     EQALEVKEGYKFLT"
     gene            27659..27910
                     /locus_tag="OQS10_000116"
     CDS             27659..27910
                     /locus_tag="OQS10_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291258.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(27982..28134)
                     /locus_tag="OQS10_000117"
     CDS             complement(27982..28134)
                     /locus_tag="OQS10_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCS2291259.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            28407..29393
                     /locus_tag="OQS10_000118"
     CDS             28407..29393
                     /locus_tag="OQS10_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291260.1"
                     /translation="MRSRYVDRIIYMKKLLIRLIPDAIYEALEKTALHSERSLEAQAR
                     YILSCSVDNEKQLTGGERYQREITARLNQALSEANEVITAINLVPARIAEQLGHHDAI
                     ESENWFTGNAVPSFTELDELSDIFGCSPDWLKFGENVPYPKSSKGRINWNRGGEKDID
                     ALLEPDNKGRKVSSIHIFRVNESGNILILREFENSITTDFFSTNLYLSDKEKIGQGGF
                     HDLVDFLVILQSLYLKYINSNLLVKSYNISQYVLDSCYKSGEKHPITICSAGETSTWW
                     EDIWHEDMIAQSRNNESYFWDGDKPLIDSLHKELQKNNRLKPNSELDMPLIG"
     gene            29611..30819
                     /gene="trbA"
                     /locus_tag="OQS10_000119"
     CDS             29611..30819
                     /gene="trbA"
                     /locus_tag="OQS10_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCS2291261.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYTSCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPKKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            30838..31908
                     /gene="trbB"
                     /locus_tag="OQS10_000120"
     CDS             30838..31908
                     /gene="trbB"
                     /locus_tag="OQS10_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HCS2291262.1"
                     /translation="MTIEYFATRIAKHISVQAIRPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVLEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            31901..34192
                     /locus_tag="OQS10_000121"
     CDS             31901..34192
                     /locus_tag="OQS10_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCS2291263.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     KTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELHPGEFFS
                     IFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(34229..36928)
                     /gene="nikB"
                     /locus_tag="OQS10_000122"
     CDS             complement(34229..36928)
                     /gene="nikB"
                     /locus_tag="OQS10_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HCS2291264.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTD
                     TDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(36939..37271)
                     /gene="nikA"
                     /locus_tag="OQS10_000123"
     CDS             complement(36939..37271)
                     /gene="nikA"
                     /locus_tag="OQS10_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HCS2291265.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            37505..37840
                     /locus_tag="OQS10_000124"
     CDS             37505..37840
                     /locus_tag="OQS10_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCS2291266.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(37926..38774)
                     /locus_tag="OQS10_000125"
     CDS             complement(37926..38774)
                     /locus_tag="OQS10_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCS2291267.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNTSKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENMLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            39124..39321
                     /locus_tag="OQS10_000126"
     CDS             39124..39321
                     /locus_tag="OQS10_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291268.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVKTEKIARHLQTKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            39355..39606
                     /locus_tag="OQS10_000127"
     CDS             39355..39606
                     /locus_tag="OQS10_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2291269.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(39637..39831)
                     /locus_tag="OQS10_000128"
     CDS             complement(39637..39831)
                     /locus_tag="OQS10_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291270.1"
                     /translation="MTLAERLIQKGFDEGFKEGFKKGALEVAREAACRLRDMGWTSER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(39894..40784)
                     /locus_tag="OQS10_000129"
     CDS             complement(39894..40784)
                     /locus_tag="OQS10_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS2291271.1"
                     /translation="MSKKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKKIIH"
     gene            complement(40849..41115)
                     /locus_tag="OQS10_000130"
     CDS             complement(40849..41115)
                     /locus_tag="OQS10_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291272.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(41208..41642)
                     /locus_tag="OQS10_000131"
     CDS             complement(41208..41642)
                     /locus_tag="OQS10_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCS2291273.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(42371..42871)
                     /locus_tag="OQS10_000132"
     CDS             complement(42371..42871)
                     /locus_tag="OQS10_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCS2291274.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(43319..44494)
                     /locus_tag="OQS10_000133"
     CDS             complement(43319..44494)
                     /locus_tag="OQS10_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2291275.1"
                     /translation="MLRATKIRIYPTPEQAAFLNAQFGAVRFAYNKALHIKKHAYQRH
                     GVNLSPRKDLKPLLSVAKKSRRYAWLKEFDSMALQQAVINLDVAFSNFFNPKLKARFP
                     TFKRKHGKQSSYHCVGIKVQDNAIKIPKLAPIEARLHRELHGKLKSITVTRSATGKYY
                     ASILCDDGASAPEKPTYLKEEKITGLDMGLEHYAITSDAVKVPNPRHLINAGRNLRRK
                     QKALSRKQKGSANRKKARIRLAALHERVANARADFQHKLSRAIVDENQAVIVETLKTA
                     NMMKNHNLARAIGDAGWHSFITKLEYKTATKGVHLVKLDQWFASSKTCHCCGYKMPEM
                     PLHKRIWRCHECGAEHDRDINAALNIRQKGILELKAAGLVVSAHGGQRKSVAQTVAA"
     gene            complement(44624..45343)
                     /locus_tag="OQS10_000134"
     CDS             complement(44624..45343)
                     /locus_tag="OQS10_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCS2291276.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(45340..45774)
                     /gene="psiB"
                     /locus_tag="OQS10_000135"
     CDS             complement(45340..45774)
                     /gene="psiB"
                     /locus_tag="OQS10_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCS2291277.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(45829..47787)
                     /locus_tag="OQS10_000136"
     CDS             complement(45829..47787)
                     /locus_tag="OQS10_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS2291278.1"
                     /translation="MSVVKSEPDTTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVDGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKAPVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQMDATEHTTNLADAA"
     gene            complement(47849..48082)
                     /locus_tag="OQS10_000137"
     CDS             complement(47849..48082)
                     /locus_tag="OQS10_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCS2291279.1"
                     /translation="MNKSDEMIQELPDGAFTRECAETVVAQYRNVFIEDDQGTHFRLV
                     VRQDGSLIWRSWNFEDCAGYWMNRYIRDFGIRK"
     gene            complement(48139..48666)
                     /locus_tag="OQS10_000138"
     CDS             complement(48139..48666)
                     /locus_tag="OQS10_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS2291280.1"
                     /translation="MSARGINKVILIGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQLLGRAPQQNAQAQPKPHQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     QQPEPPYGFDDDAPF"
     gene            complement(49050..49364)
                     /locus_tag="OQS10_000139"
     CDS             complement(49050..49364)
                     /locus_tag="OQS10_000139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291281.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDAVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPV
                     AP"
     gene            complement(49435..49626)
                     /locus_tag="OQS10_000140"
     CDS             complement(49435..49626)
                     /locus_tag="OQS10_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291282.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQDSRNR"
     gene            complement(49623..50045)
                     /locus_tag="OQS10_000141"
     CDS             complement(49623..50045)
                     /locus_tag="OQS10_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCS2291283.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMVEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(50092..50517)
                     /locus_tag="OQS10_000142"
     CDS             complement(50092..50517)
                     /locus_tag="OQS10_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS2291284.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKQMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(50928..51704)
                     /locus_tag="OQS10_000143"
     CDS             complement(50928..51704)
                     /locus_tag="OQS10_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291285.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVQSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            complement(51750..52184)
                     /locus_tag="OQS10_000144"
     CDS             complement(51750..52184)
                     /locus_tag="OQS10_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCS2291286.1"
                     /translation="MYGTCETLCRELAAKYPGNTPLMLLIWSPEEIQALADGMEISLT
                     DHEIRTVLAHLEDIPEDQRIESGISSAAAMEIISNVSENRLVTVSAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPESVTRRQAVVNQARSLLKNNTHEND"
     gene            complement(52197..52418)
                     /locus_tag="OQS10_000145"
     CDS             complement(52197..52418)
                     /locus_tag="OQS10_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291287.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(52419..53102)
                     /locus_tag="OQS10_000146"
     CDS             complement(52419..53102)
                     /locus_tag="OQS10_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS2291288.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(53178..53483)
                     /locus_tag="OQS10_000147"
     CDS             complement(53178..53483)
                     /locus_tag="OQS10_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291289.1"
                     /translation="MTETGGKPPVSFPTKDVAVLLFLLRRLTRRGINAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(53487..54389)
                     /locus_tag="OQS10_000148"
     CDS             complement(53487..54389)
                     /locus_tag="OQS10_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCS2291290.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TEQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            54807..55055
                     /locus_tag="OQS10_000149"
     CDS             54807..55055
                     /locus_tag="OQS10_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS2291291.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            55052..55489
                     /gene="umuD"
                     /locus_tag="OQS10_000150"
     CDS             55052..55489
                     /gene="umuD"
                     /locus_tag="OQS10_000150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS2291292.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            55489..56763
                     /gene="umuC"
                     /locus_tag="OQS10_000151"
     CDS             55489..56763
                     /gene="umuC"
                     /locus_tag="OQS10_000151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HCS2291293.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGELQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(56765..57181)
                     /locus_tag="OQS10_000152"
     CDS             complement(56765..57181)
                     /locus_tag="OQS10_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS2291294.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(57174..58154)
                     /locus_tag="OQS10_000153"
     CDS             complement(57174..58154)
                     /locus_tag="OQS10_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCS2291295.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(58568..58876)
                     /locus_tag="OQS10_000154"
     CDS             complement(58568..58876)
                     /locus_tag="OQS10_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HCS2291296.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(58963..59607)
                     /locus_tag="OQS10_000155"
     CDS             complement(58963..59607)
                     /locus_tag="OQS10_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCS2291297.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(59787..60566)
                     /locus_tag="OQS10_000156"
     CDS             complement(59787..60566)
                     /locus_tag="OQS10_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCS2291298.1"
                     /translation="MQYLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPVGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVTAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(60568..60981)
                     /locus_tag="OQS10_000157"
     CDS             complement(60568..60981)
                     /locus_tag="OQS10_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291299.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            61549..61806
                     /locus_tag="OQS10_000158"
     CDS             61549..61806
                     /locus_tag="OQS10_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291300.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            61806..62396
                     /locus_tag="OQS10_000159"
     CDS             61806..62396
                     /locus_tag="OQS10_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291301.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            complement(62414..62761)
                     /locus_tag="OQS10_000160"
     CDS             complement(62414..62761)
                     /locus_tag="OQS10_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291302.1"
                     /translation="MTFEQKKARAIALMDSKNMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFHWW
                     RRKVNRLPSWDDV"
     gene            62880..63227
                     /locus_tag="OQS10_000161"
     CDS             62880..63227
                     /locus_tag="OQS10_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCS2291303.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            complement(63245..65125)
                     /locus_tag="OQS10_000162"
     CDS             complement(63245..65125)
                     /locus_tag="OQS10_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HCS2291304.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNERAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNVSARTNEQKHANDALNALLKEKENIRSQIADINHKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYNEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     SIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWTFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(65404..66750)
                     /locus_tag="OQS10_000163"
     CDS             complement(65404..66750)
                     /locus_tag="OQS10_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291305.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMVLHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            67104..67706
                     /locus_tag="OQS10_000164"
     CDS             67104..67706
                     /locus_tag="OQS10_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCS2291306.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIITPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            67762..68256
                     /locus_tag="OQS10_000165"
     CDS             67762..68256
                     /locus_tag="OQS10_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291307.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIIY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNNDTPKKSTVPNEDK
                     KGIR"
     gene            68256..68522
                     /locus_tag="OQS10_000166"
     CDS             68256..68522
                     /locus_tag="OQS10_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291308.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNKANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(68714..68995)
                     /locus_tag="OQS10_000167"
     CDS             complement(68714..68995)
                     /locus_tag="OQS10_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291309.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(69101..69376)
                     /locus_tag="OQS10_000168"
     CDS             complement(69101..69376)
                     /locus_tag="OQS10_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS2291310.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(69376..69660)
                     /locus_tag="OQS10_000169"
     CDS             complement(69376..69660)
                     /locus_tag="OQS10_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCS2291311.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEDRNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(70564..71595)
                     /locus_tag="OQS10_000170"
     CDS             complement(70564..71595)
                     /locus_tag="OQS10_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS2291312.1"
                     /translation="MAGLKNTPYNAVHWCQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            72568..72831
                     /locus_tag="OQS10_000171"
     CDS             72568..72831
                     /locus_tag="OQS10_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS2291313.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            72749..73036
                     /locus_tag="OQS10_000172"
     CDS             72749..73036
                     /locus_tag="OQS10_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HCS2291314.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            73478..74011
                     /locus_tag="OQS10_000173"
     CDS             73478..74011
                     /locus_tag="OQS10_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCS2291315.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            74265..74948
                     /locus_tag="OQS10_000174"
     CDS             74265..74948
                     /locus_tag="OQS10_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HCS2291316.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            75120..75374
                     /locus_tag="OQS10_000175"
     CDS             75120..75374
                     /locus_tag="OQS10_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCS2291317.1"
                     /translation="MPQQHPRRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            76126..77193
                     /gene="pilL"
                     /locus_tag="OQS10_000176"
     CDS             76126..77193
                     /gene="pilL"
                     /locus_tag="OQS10_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HCS2291318.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSATAPAQDTATTISPAAMVTTSTSPESSTTANSQN
                     KPRNPFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            77193..77630
                     /gene="pilM"
                     /locus_tag="OQS10_000177"
     CDS             77193..77630
                     /gene="pilM"
                     /locus_tag="OQS10_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCS2291319.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQQMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            77644..79326
                     /locus_tag="OQS10_000178"
     CDS             77644..79326
                     /locus_tag="OQS10_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCS2291320.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            79319..80614
                     /gene="pilO2"
                     /locus_tag="OQS10_000179"
     CDS             79319..80614
                     /gene="pilO2"
                     /locus_tag="OQS10_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCS2291321.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGEISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTA
                     EEIAARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIQEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            80601..81053
                     /gene="pilP"
                     /locus_tag="OQS10_000180"
     CDS             80601..81053
                     /gene="pilP"
                     /locus_tag="OQS10_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCS2291322.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVNLSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            81064..82617
                     /gene="tadA"
                     /locus_tag="OQS10_000181"
     CDS             81064..82617
                     /gene="tadA"
                     /locus_tag="OQS10_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCS2291323.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDITPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     VMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSED
                     DRAYLERHCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDA"
     gene            82630..83715
                     /locus_tag="OQS10_000182"
     CDS             82630..83715
                     /locus_tag="OQS10_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCS2291324.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPVLSKI
                     SSPDSWSGALGFLYGLSLFVDNYGAICAVLFAVVTGVISWSLKNWKSRSLADNIMPWS
                     IYKDIQGAAFLLNMAALLKAKMTTLNSLNVLQEFASPWLSTRLDSIIYRVRQGDHLGL
                     ALRQCGYHFPSRESANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLI
                     FLVMSLLLLVMAVVDIQSIGDNSMGSI"
     gene            83732..84346
                     /locus_tag="OQS10_000183"
     CDS             83732..84346
                     /locus_tag="OQS10_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HCS2291325.1"
                     /translation="MLIQNINANVLKKNEKETSHDKGWGIMEQGAIALVVIIVLALVF
                     GGLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGAKMTGALIQMGGVPKTM
                     TVRGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTG
                     LTNGITLNSTAHSDGKVTTEEASAQCTADNGSTGTNKLIFTING"
     gene            84356..84916
                     /locus_tag="OQS10_000184"
     CDS             84356..84916
                     /locus_tag="OQS10_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS2291326.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIK
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKVWRIYSDMKGICLPGQGGRQCRQS"
     gene            84901..85557
                     /locus_tag="OQS10_000185"
     CDS             84901..85557
                     /locus_tag="OQS10_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS2291327.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWY
                     KTHTAVLSGIWLYSIAVALIILSSSPLIYRIHAVLILSFMLQMAVTDALTGLLPGTFT
                     RRFLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI
                     "
     gene            85545..>86618
                     /gene="pilV"
                     /locus_tag="OQS10_000186"
     CDS             85545..>86618
                     /gene="pilV"
                     /locus_tag="OQS10_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCS2291328.1"
                     /translation="MGNLMKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGW
                     QAEARLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSE
                     GQRLQAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATG
                     ALRSWSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAI
                     DMGSNNLNNIGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGW
                     LITRNSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQ
                     LERTAVAGASCSPNGLVGRDNTGA"
BASE COUNT        22476 a        22318 c        20999 g        20825 t
ORIGIN      
        1 attgttggca ttattttttc ctcttttgag gttagcatgc cgtctccacg catccgtaaa
       61 atgtccctgt cacgcgcact ggataagtac ctgaaaacag tttctgttca caagaaaggg
      121 catcaacagg agttttaccg gagcaatgtt atcaagcgat atcccattgc ccttcggaat
      181 atggacgaaa taacaaccgt tgatattgct acatacagag acgttcgttt agcagaaatc
      241 aacccccgaa cgggtaaagc cattacaggt aatactgtac gtcttgaact cgcccttctg
      301 tcatctctgt tcaatattgc tcgtgttgaa tggggaacct gtcgtactaa cccggttgaa
      361 ctggttcgca agccgaaagt atcctccgga cgagatcgtc ggctaacgtc ttcagaagaa
      421 cgtcgccttt ctcgctattt ccgcgaaaaa aatctgatgt tgtatgtcat tttccatctt
      481 gcccttgaaa cagccatgcg gcagggcgaa atactggcct tacgttggga acacattgat
      541 ttgcgccacg gtgtggctca tttacctgaa accaaaaacg gtcactcacg ggatgttcct
      601 ctgtccagac gtgcccgtaa ctttcttcag atgatgcccg ttaatctcca cggcaatgtt
      661 tttgattaca ccgcatccgg ctttaaaaat gcctggagaa tagccacaca acgacttcgc
      721 atcgaggacc tgcattttca cgatctacgg catgaagcaa taagccgctt cttcgaactg
      781 ggtagcctga atgtaatgga gattgctgca atatcaggac atcgttccat gaatatgctg
      841 aaacggtata ctcatcttcg tgcatggcaa ctggtcagta agcttgatgc ccgccggcgg
      901 cagacacaaa aagtggcagc atggtttgtg ccgtatcctg cccatatcac gaccatcgat
      961 gaagaaaatg ggcagaaagc gcatcgtatt gagatcggtg attttgataa ccttcacgtc
     1021 actgccacaa cgaaagagga agcagttcac cgcgccagtg aggttttgtt gcgtacactg
     1081 gccattgcag cacagaaagg cgaacgtgtc ccatctcccg gagcgttacc tgttaacgac
     1141 cctgactaca ttatgatttg ccctctgaac ccgggcagca ccccgctgta aacatcattc
     1201 aaaacacata cagaaaggcg agttgcaaca actcgccaaa cacattcgat ctgcatgatc
     1261 gaaattattc gtttcaccag aaaaaacacc gaagatacaa taaccacatg agctgaaaag
     1321 actttttcag tcggaggaac aatgagactg aatacgaccg gtattgcggc ccgaatgatg
     1381 ctctcgctgg acaaaaaagc aatcggcgaa aaactcatca acggtcgtca gataaacccg
     1441 acacctctgc aggtccgtta cccacaaggg gtaagagaag ttctggggat aatgagcgaa
     1501 caattagcca tatccaccgc tgatttaacc cgaatcctgc ttgaggatgc tcttcataat
     1561 atgtttatgc ctgcggataa caccacagga aacataataa gcagaattga atacatcatg
     1621 ctgtcgcatg atataaatgc gccattactg gcaacattgc tgtcgccatg gaatatccgc
     1681 tccacggtta tacaggaccc ggccagactt gcagactatc tttcagctga cgctctcgaa
     1741 catcttgctg aatgttttaa tctcaatccg gactggctga atggccatga aaactatccg
     1801 atagcgttgt ccggtgaatg gccagatact gctgataatt tcagaatgct tattaataat
     1861 agcagcaaca ctgaagttat tttctggcat agtttcccct tcgcaggaaa cacgaaaaga
     1921 gaatattgcg gggtaatatt gagacaggaa aaagaaataa atggctctgt aatatatccg
     1981 gcactatcat tatcaccaac acttctgaac gatgaaaaaa gaaaatggtt aactgaatat
     2041 acaaccaggc agaatacaac aatgtctttg cgacgcgtca cgttgagacc tggccttgcc
     2101 gagaacctta ttacagggca aatactacct gttagcttat ttaatacgtc tttattaccc
     2161 tggtaaatac cagatagtac caaagtaaaa aattacggca catccgtgtc atagcgttgt
     2221 tttgtctaaa ttctgataaa caggaaatta aatgatgaag aaaaatcata ttacccgcac
     2281 catcattgca tctgctgtat tattttcctt taacgctgca gcagcgacgt catattttga
     2341 agcgcgtaac gatgcaatgg gtggcaccgg ggtagcatct tcccattatg gtgtggcacc
     2401 tctggcaaac ccggctttac tgacaaagca caacagcaat gatgatttca gccttcttct
     2461 gccgtctgtt ggggcacaag ttgctgatcc taatgatgta tcaaataaag ctgatgatgt
     2521 taaagatgac tgggatttgt ttgacagtgc tgtagataac cagcatggcg tacaacaggc
     2581 agcggcaaat cttaaacatc gtctgcagga attccgcaat atcaatgcag atgctcaggt
     2641 aggtgtatca gctgtagcag ccatggcaaa tgacactctg ccgtttgccc ttatggtcaa
     2701 gtcttacgga actgtcagcg taaacggaaa agtaaacgat gcagacctgg attatctgga
     2761 caaagttgcc aatggaacaa ttaccgacgt cgataagaat gccctgacct cacgcgcatt
     2821 cgggcgtgca gctgtaatta ccgacgtcgg aatttcattt gccaaagaac tggaaacggc
     2881 cggacaaaaa tggtctcttg gcgtcacacc aaaatatcag cgtgtggacc tgtttaacta
     2941 caacgtaacg gttcgtgatt acgacaaaga cgattttgac ggtgacaagt accacaacac
     3001 gaaaaacgga ttcaacgccg atattggtgc ttatacagac ctgaatgaca actggactgt
     3061 cggtcttgtt gcccagaaca tcattccacg cagtattgat actaaagtcg tcaatggctt
     3121 taaagaaacc ttcaaagttc gtccacaggc aaccgccggt gtatcctggc ataatgacct
     3181 gtttaccact gctcttgacg ttgatctgac tcctgccagt ggcttcactt ctgacagcaa
     3241 acgtcagttt gcaagtgttg gtgctgaatt taacgcatgg aaatgggcgc aattgcgtgc
     3301 cggttatcgt cagaacatgg catcaaactc cggaagcgcc ttcaccgctg gcgttggtat
     3361 ctctccattt gacgtcgtac atatcgatgt ttccggcctg gtaggaactg accacgatta
     3421 cggtgcaatg gcacaacttc agttcacttt ctgattactg tccccgcccc cggcactaaa
     3481 cccgggggta tttttatatt cattttaaat aacatgatga aaccacgttc ttcttattca
     3541 aagacagcat tcattctttt attttctgtt ttcctggtag ctgcagtgac aaaagcaaaa
     3601 agctcgcttc ccgatatcac cctagaacag gcaaaagaaa ttaatgctga taacaccgtt
     3661 attttccttt tccgccatgg agaacgttgc gaccgttcag atatgccatg ctattcggat
     3721 aaaagcggaa tcactattac cggtacagag aaagcacagc aggaaggtat taagttcgca
     3781 acaatcttca gtgaatatga tatttactcc agcaatgcgg taagaaccat acaaactgca
     3841 aaattcttct ccggtaaaga ccccgtcgtt atggacagtt tatcagactg caataatgat
     3901 ctttataaga cgcttgaatc aatcgtaaga gaatctcata aaaggaacat agtaataatg
     3961 acacataatc attgtttatc atttctggcc agagataggc tcggcaagaa attcaaaccg
     4021 gcgtaccttg atgccctgat catgcattac gatggtaccc ggctcatact tgacggaaaa
     4081 tacaacaaag aggcatagag gatatttgaa tttacataaa ttctgatata ctcagtaaga
     4141 tacaagagtg tacgggcaca tgtgcctgtc tgaattgcag gtcatccgga cgagggccgg
     4201 gaactgtttc agggacaagc gccggtacgt tcgattcata aatcgtcgag tcatccatca
     4261 tggatcgatt cggcgcggag cgttaaagat tacgctcata ttcgttttat gtctgacgac
     4321 acatcaacca gccagtatca ggaataatca ccttcagtaa actatgaggc tatttacctg
     4381 gccacactgg catttatttc cggtaacgca agtatagccg gaaacaaaca tcatcctgtc
     4441 tgtaccggag caccggtata tcccattctg atttaactgc tcaggaatta ttatgaaccg
     4501 tattatacct gcggggtgtc ttgccgccct ttttctggct ggctgcaact ccccttctca
     4561 gcatactcct gttccgcgta actacggtgc tgcctctgat gtggcggtta cccggcacac
     4621 tcagtacaca ttatgggaca aaggtgtgtt ttccggccaa cttccggttt caacggggtc
     4681 tcatcttctg acagctaaca gcgatcgcct ttcctttgac tgggaaggtg atgcaattga
     4741 attactgaac gaactggcca gagtcagggg gatgcagttt aactacaatg gtgtccgcct
     4801 gccgttacca gtcaaccttc acgtccggga tatgacgttt tccaatacgt tgagacttat
     4861 tgaagcccag acagcctgga gagcaacaat ccatcaatat ccgggcttgt tacaggtcag
     4921 cttcatgcag ccggagaacc ggaaaaaatg aaagcactca cctctctcat tttgccagta
     4981 atactgctta ctggttgttc ttcttcccct accatctcgc caggtggtgt caatacgcca
     5041 ccacctgaca ttaatgcatg gcttaaacca cgaagtgaaa agccggatag tctgtcagaa
     5101 acacgctgga aaatgctgac cgacgcaggt aaaacgcttg gatttcgtgg tggcaaggct
     5161 cagcgttcat gggagctgat tcaggcactc aacgcccggg aaagcacact taatgcctta
     5221 tacgatttcc ggccactgat tagcccggaa ggctggctac caccggttat tgatgaagca
     5281 caggacgtag ctcatatcac gccagatcaa atccggacct cttcccgggt ctggaccatc
     5341 attcgccctg aacgctttgt cagcaatcca ccgggctggc gtgactggct tctccggggg
     5401 ctttcaacaa cagccactcc gggtacagaa ggcagcgtcg ttccagaaga cagcgtgcaa
     5461 cgaaaagtgt gggaaaccgc attacggcag ggatggcagg aaggaagaca aaacgccgac
     5521 ctgacgcttg aagccaacca gaaaaccctc acccgtgatt accggggcat gatgctgtac
     5581 tcacttctct ggcgtcaggg catgatcacc cgtccggatg tttctgatca aatgcaaacc
     5641 gttacaggtg acgggaaaaa actggtcact ggagatcgtg ttcgtcgcct taaaaatcat
     5701 gcggaattca atcttcagaa atctcactgg cgtccgctta taggcactga aggaggctcc
     5761 cgatgaatat tcctgatgag tttggtcttt ttccgtttca gcgtttcact gcagatgaat
     5821 tacggcattt ctttgtctgg tgtgccgcac ataaagtcag tgatgtggat ctgaccggtg
     5881 ggtcacctgt atctgtcagt cgctttggcc gacgggtgcg ttgttccagt gcaacgttac
     5941 caaccacact catgtcctcc ctgattgatg aactgttcgg tcgggaagtc gttccccgcg
     6001 ttctggcagg caatccggtt gacaggacga tacagattaa cggggatgca tccggtcgct
     6061 atggtcttaa acgcggagaa cgcatccggt tacgctccca cctcattcag ggcacatctg
     6121 gtgcagagga gaaagcagtc tcaataacca tgcgtgtcat cccgacagaa ataccggaca
     6181 ttctctccat gaacattgaa ccagacctgc ttgaagcaat ggtctgcaaa agcggtctgg
     6241 gatttgtatg tggtgaaacc gggtcaggaa aatcaacgct gtgttcagca ctgtaccgtt
     6301 acattatgga caatttcccc gacgccaaaa tcgtgacata tgaagacccg gtggaatata
     6361 tcctgggaaa tgaaaacgat ctgctcccac cgcatcaggc agaaatcggg cgtgatgtgg
     6421 tcagttttgc tgccggactg cgttctgctg tacgacgtaa tcccgaaatt atcggtgttg
     6481 gtgagatcag ggataacgaa accgccgacg cagcggtaca ggcaggcaac accgggcatt
     6541 actgcctgag caccatgcat acgaaatctc ctggtgaaac gctggcacgt cttcttgggc
     6601 tgtttccccc tgtcatcagg gattcgatgg cctgggctgt cctgtcactg ttgcagttca
     6661 ttctggttca ggttctggtc agaacaaatg acggtggtcg taaagccgtc cgggaatata
     6721 tcgtcattaa cgatgagctt cgcgacaatc tttccggaat gccgcatgca gagtgggggc
     6781 atcacattga tgccataatc cggcaggaga agcgccgtat ccgggaccag attctggaaa
     6841 tgtatatccg gaatgaagtt gaccgaagag aagcgattct ttttatcccg ccaggggagt
     6901 tacggtcatg aaaagagaag gtttctggcg atatgcaaca ccgccagtca acatccaggg
     6961 cgtacctctg ccgttcacca tgatttatct gtttatatgc ccctttccct ccaaaacaac
     7021 cttctggatt tgcaccggca tcattttatt tttcgtcatt ctcgaccgtt caggctggac
     7081 tgtcaggacg atctgtactc gcattttcag cattctgaga ggggccatcg cctccggtcg
     7141 tccatggtgg tatcgccacc atacggaatc accacgagac tggactggcc tcgattaaat
     7201 tctggagtct ttatgtcagg taaaaaaact atcgttactc tgctccgggt atcgctactg
     7261 gcatgcccgt tactgttcac gacaccatcg tttgcaatga tcgacacgcc atccgtgaag
     7321 gtcggctttt ctcctgaagg aagcgcatcc gctctggtgc ttgacacaat caacagtgcg
     7381 gaaagctcca tcaggatgat ggcatattcc ttcaccgacc cggatgtaat gcatgcgctg
     7441 gcaaaagcga aaaaacgcgg agtggacgtc cgtattgttg ttgatgacaa ggggaatacg
     7501 aaccgggcaa gtcaggaagc gatgaaatat atcaacctgc tggacattcc tctccggact
     7561 gtagatgcct tccccatcca tcacgacaaa gtcatcattg ttgacggaaa cacggttgaa
     7621 actggctcct ataacttctc tcgtgctgct gcacgcaaga attcagagaa tgtcgttgtg
     7681 ctcaagaata tgccggatgt agccgcacaa tatcttgaac actggcagga tcgctggaat
     7741 aagggtacag actggagacc ctgacaaaca tttctccccc gattttctat ccccccagcc
     7801 acccccggct tccggcggtg gtttttttcc gtcttttttc aggagaagca ctcatgccgt
     7861 ctgtatcccg ctaccgcacc tggcttgccg tgcctgcaga tgaaatagaa gatctcaaaa
     7921 aagcacatcc cccgatgaac gggcacacgc ccgtcatctg ggacaaggag cacaaactct
     7981 ggttcgcccg cccgggggcg gacctgtcca ggctggaccg ctggctgccc cggccacagg
     8041 acgtctccat gaacggcagt gatccggtca ccgagtttgc ccaggttctg gagaatgccg
     8101 gactggttct gaaagaactg ccggtcatgg acgggaaaat ccaccgtgtc ccgaccgccg
     8161 atgacaaaaa aggacaaaaa agtggcgcat atcgcggatt cctggacggc agacctgccg
     8221 gctggtacag ggattatcgc agcgcggata attcgccgat cacctggacc ttctccggcg
     8281 gagaacagac agatccccgc gcaagactcc acctgaaagc gcattcaatg caacgcaggg
     8341 aggacgcgga gcgggaactg aaagcacagt acaaccgaca ggccgcgtat gcccgccgct
     8401 atatcaacaa atggccgcag gccacagcac atgaatacct gacccggaaa ggcattcagg
     8461 ctgcaccggg ggtgcgggta aacaacaaaa atgaactggt tattcccttc agtaaccgta
     8521 acggcgcgat acgctcttac cagcgtattc cggtcaccgg ggggaaagat gcccgcatcc
     8581 tgatagattc ggagaaaacc ggtaactggt ttgcgctggg cacaccgcgc aacggtcagc
     8641 cggtactgtt tgccgagggc tatgccactg cggcctccct gcacgaagcc acgggccttc
     8701 cggtactgat gacggtggat ggaggcaaca tgattgccgt tgccgaaaat gcccggcaaa
     8761 agtggacgca aagccccttt atcttctgtg ccgacaacga tcatgcaatc cgggttaaca
     8821 aaggtatcgt cagtgcaaca aaagccgcag aactgacagg aggatcggtc atctttcctg
     8881 cgttcaccga tgccgaaaaa gcgcaggggc tgaccgattt caatgacctg gacgcaagcc
     8941 ggggacgggc cgcttttcag catgtcatca acgcccagtt agaacacatc ggggtttcca
     9001 cccccaacag taacaccccg gaaatgcgcg aggcactggt gataggcaac ctcgttttta
     9061 caccggtcca cactgaggaa aaaaccatga ctccgacaga atatcctgaa acatcacccg
     9121 atacagggca cagccatgac cagggacctt ctctgccgtc cgccacgcag caggaacaac
     9181 cagctgcttc cagcagtaac attgctgatg aaacacagtc atttaccagt cacgccactg
     9241 aaaataacgg caaagatgag cggcacgctg ataacgttca ggcgcaaacg gaaagctctg
     9301 tagttcagga ctcccctgca gaatcactgg cgtccccgga aagtacaacg gcgtctgccc
     9361 ctgatgaacc cgctcatccg gcggaacctc cggaaaaagt tgtctcggtg gccacagata
     9421 aagactggcg tgaatttgaa gctgaactgt cacaaccgga aaaaaacgag tcacaacagg
     9481 aatccggcac ttcacctgaa ataccggccc ctgctgccac caccgcatcc cctgaagaca
     9541 gtccctcatc tgaaaccatg aatgaatcag ccgagccatc accttcccct gatgcagtca
     9601 aagaggaaat gaccgtgatg acggagcctg atgacaaaca aagcaccgga atatccccgg
     9661 atactccccg ggaagaggcg gtcaaccatg taactgaaga cattcccctt cagccagagc
     9721 cgttccttcc tgagtcagat ggccccgaag aatacgaaaa ctacagcgca tatcaggagc
     9781 tgatgaacaa tgaaaatcct gagcatctgc aggaaaacag tgatatgccg tcgcaaccgg
     9841 acacaggcca cgttcaggct gatgagtcag aacttcaggc cacaacagat acagtagaac
     9901 cggctgtccg ggatgacccc actccacagc aacctgttca gagcacatca tcttctgata
     9961 atacttcatc gtttctcgac aaagcgcgtg gcttttttac gcgtaaaaaa acggattcac
    10021 aggcgcatga gaacagtgat cccacaccct caccggaaac cacgacgacc acaccggcac
    10081 ctgacagtat tgtctacgcc ccggaacgcc ctgatgcacc gatatcgctc aatctggacg
    10141 aaatcattaa atcgctggaa ggtgaagaac gagcagacag aaccgtcctg tacaaactgg
    10201 atggcaaacc tgccttcatt gaccgggtaa accggctgga aatggtcaac ggtgccagca
    10261 atgacgatcg cagtgttctg gctgcacttg ctgtggccac tgatttttat ggtggtgtca
    10321 ttgagctgac gggcagtgat gcctttaagc aaaaagcgat gcagttgatc atcgagcaca
    10381 acatcaaagt gcgcatgaaa ttcccggatc agcgagcggc gctcgaaaaa ttacgcaagg
    10441 aaatggctgt cggtaaggat actgttgtca cccataagcc aacaccggaa cttaaccgga
    10501 atacccctga acaaccagca gtcccagacc ctgttcagga aaaagaagcc acacaatccc
    10561 cggcgtcccc agtagcccct gaagcatcaa ccgtcagcac cgtccctgca tcatcaccgg
    10621 ctgaacctgg caaaacggca gatgcggcac aaggtgaaga accaccagga aaacttcgcc
    10681 cgggagagag cgtgactgcc gtactgcaca attttggtcg tgctgaatat gcacccggca
    10741 aaggagagag tttctttgtt gagcttaaaa accgcagtgg cagcaaactc tactgggggg
    10801 aacaactgga aagtctggtc aaaaaccatc agaaaggtga cgtggtgaca ctgacacttc
    10861 agaaccgcga acaattcatc ctcccgggag agcaaaaagc acgtttccgg aataagtggt
    10921 ccatggagtc agttaccaac gggatctcgg tatcacatga taatccggat aaaggtcagc
    10981 gtattcaggc gattccggtc gaaaccttta tgaaagtggc tgcgcaaatc agccaggggt
    11041 ggcccgaaga gatgaaagcg ttacgtatgc cagaaaacgt cggctcacat ctgtttatcg
    11101 gtgaagaccg acatccggtc tccgcgccac agaacgcaaa tcaggtaaca gaaataacca
    11161 gcgcagcccc tgacaagctg acacctgttc tgggatcagt ggataaggat acgcgtgaac
    11221 tcaacctgct acttgtccag agtgccgatg aacatctgca gggtgtggtc cggctgaacg
    11281 gcacattgta tccggcactg gccaccccat cagccgacaa cagtcagctg gttatcaatg
    11341 cgctgaccga caaaggttta cgctttgcag gttatggtga agcggtaaac cacgatgctg
    11401 acagcacgaa tcgccctgca ccggagctta tgcagtttca tctgaaaacc cgggaagaac
    11461 cgctttttgc tgctgtctac acgccggaaa aacaacctga tgcgttgtac cggaacctgg
    11521 gcttcgagca gtcatggcag cagtggtcca acagccaaaa gccggaggac aggcaggaaa
    11581 aaactctgca tcaggatctc tcccacagcc ccggccgatg agccggtaag gaagccatat
    11641 gaacatgcat aagtctctgg ttgccgtgct cggccttatc ctgatttcat ccacggcaag
    11701 tgctgcaacc tgtcgtgcgg gtagcgcggc acaggccggt agtaatacag gatatgagcg
    11761 agcacgtgca gctgcggatg catggtcaca acgtgaaaac gatgtttcct catccctgca
    11821 gtcctgtctg tcacgaatca aaaaaatcag cataaacctc ccccagttcc ccagccttga
    11881 cgacattctg tcgcagctgg aaaaccaggt ctgcgacgcg gtcgtggaca aggtgaatga
    11941 aaagctgccg gggaatattg atccctggaa ggactacaac ctatgaccga aaataccgag
    12001 ccatcctcgc catcacatcc ggcacctgac ccggaaatac tgaagagcac aatcagggct
    12061 atgaagcagt cagaacagcg ggcaaataat gtccctgcgc tgataaaagc gcttttatgt
    12121 acgggcacat gtcttcttat cagtattact ggtaacgcca ttcagtactg gcactcaacg
    12181 aatgtggagc gcgaatattt cgctacagac aatggtcgtc tggtcaggct ggcaccaaca
    12241 agtcagccag catggagtca gaatgatgca atggcctttg gcagccaggc actggcgacc
    12301 gcattcaacc tggattttgt gcactatcgt tcacagatct cctccctgtc tccgcgtttt
    12361 tctgacgaag gttttgtcgg atatgtgaat gcattacagg catctaacat tctggagacg
    12421 atcaagaaag agaagatgaa tctgaccgcc acgacaggtg ctggcgttct ggtacgacag
    12481 ggacaaatgt ccgacggcgt atggttctgg acattccagt atcctgtccg tatgcgtctg
    12541 gtcggccaga caaccagcaa gccagaacag tcttttgttt tcgaaatcac catccagcgt
    12601 gttgacccca gactgaaacc atccggtatg gaaatccggc agatgatctc ccgtaacgcc
    12661 ggaccgaact cctgagataa gtaatatgca atcacgttat ttactgagca cactgctcct
    12721 ggtgtgcagc gccgccacgt ctgcagataa cgcaggatgg cagaacgcac gcacaccaca
    12781 aacgacaaat tctgccagcg atcacgttca gaacacctcc caaaacaccg gaggtgttcc
    12841 ggcaacactg gtaaaaggtg agctgcctgc ccccgggcag gccagcccgc tggtacagga
    12901 tgcggcaagg cttgattctg agctctccgc cgatgagatt cgctcactac gcagtctcat
    12961 ggcggataac gagcgggcca tcaatgcgcc catcaccagt gttgtacccc gtatcagctc
    13021 tctgacagtt aatctgtccc ccggtgccag ccttcctctt gttcgcacag ccatgaacaa
    13081 cctgagcgtg gtgacattta ccgatatcaa cggttcacca tggccacaat ccgatcctcc
    13141 ttataacgcg gcaccaaaac tctttgatgt gcagtacaac gagaacatgg tgacaatcac
    13201 accattgcgg ccatgggcat ccggaaatat ttctgtctac ctgaaaggtc tgtcggtacc
    13261 ggtcatcctg aacgtaacca gtggcgaaac agatacgccg tcatccagtc aggaaatgga
    13321 cagtcgtctg gatctgcgaa taccacggca gggaccaacc agcccagttg tctccatccc
    13381 gacggataag atcgccctgc atgatgcaac attacaggca tttctggatg gtattcctcc
    13441 ccgggaccct tcagttaaga ggctgaaatt cacaggtaat gtcccggata caacgatctg
    13501 gcaacacggc gatgaccttc tggttcgttc ccgggcgatg ttgcgcgatg aatttgaaca
    13561 gaccttgtcc tctgccgacg gaactcatct ctggaaactt cccgtcaccc cactactgac
    13621 tttctctgtg aatggccagt ccgttcacgt gacaccagaa ctggagtaat catgagtgct
    13681 gaacaggatg ccggtaaatc cggaaagaaa ctggctgccc tgcttggact gggcagcatc
    13741 atcttatttg gcggcggcta tatcgcgttt tccaaactat cagggagtaa cagcgatatg
    13801 cagtcagccg tgaacattaa cagtgcagca agcggcggaa cgcgcagcgt taccgaaacg
    13861 cctcactatc gtgaactgtt aagagcggat aatgaacgag gggccgcagc tgcagcccgc
    13921 aataatcaga cgttcattgc cagtcttcca caggggcttg atattccgga cactccggaa
    13981 aagcaacaac agcctgcagc aaaaccggaa aactatgctc atcgccaggc ttccggcaca
    14041 ccacaggagg atcgagcagc cagtgaaaaa cgtatggagc ggctgcaaaa gctgattgta
    14101 agaataaaag atcagcatcc tgccggtagt acaccaacca tagcaacgac tatgtggaat
    14161 aaatctccgg cagaaacaac agggcagaat ggtacgcagc aattcgcatt acagaatgcc
    14221 agcctgtcca cgcctgtagc agaaaaaggg atacaactga tacctgctct gacccgtata
    14281 ccggcatata ttgatactgc agtggattct gataacccgt catccaaggt tatcgcaacc
    14341 attcccgctg gcccctgggc aggggcaacg ctgttcagtc cgggagtcaa actggtgggt
    14401 aacggcgtgg agatccattt cgaccggatg agctggaacg gcatggacct taaggtcaat
    14461 gcatatgctc aacgggaaga caacctgatg tcatccgtgg ccagcaatgt gaatacacgc
    14521 tggtttaaac atatcattct gccgtcagtg ttaggaggtg tgggcagtat tggcacactt
    14581 tataaagatg ccaacacgca ggttattcag ggtaactatg gcacggttac cggacgcgtg
    14641 ggaatgccca gtggtgaagc tgttgcgggc gttattgccg gtggtatggc ggaaagagga
    14701 agtcagatat taacccgcca ggcagaaagt gagccctata aacaggtaga agtgtatcag
    14761 catgaagtcg tatcaattct gtttgttgac ccggtaatga caaacgacgc caggtcatct
    14821 tccctttcca gtggcatttc cccttcagtt aatcgtacat cacaggcgga acaacgctcg
    14881 caggcacgaa tgcagacagc catggagcaa agaaaagctg tgatgcaacg tcggtatgat
    14941 gaacaaccgg agacaccata atgaaacctg aaacagaaat agatgaaacc ggcagcttcg
    15001 gtgaacccga agagaaaccc gcccccttct ggaaacgttc cgtctggggg atatctgtag
    15061 caacatgggg gctatgtgca gtcgtgttgc tcgcagccat atggtatctg ttcctgagag
    15121 cgccatcgga aacaggcatg cctccgttta atgatgcgga tgctggcgta cagacctggc
    15181 aaaccacaca agaatcatca ccttccgttc agtccactga gactatgact gtccggacag
    15241 gagaagatat gagccagctt gcccgggatg taaaaacaga gctggataac cgcgatgaaa
    15301 aaattcaggc aacactgaat atgcttcacg acagcattaa caagctgggc gaagctataa
    15361 aaaaggatga agaatacgca caggaaacac gccgtcagct ggatgatatc cggagccgcc
    15421 tgaacggaat aatgacacag aaaagcgtca ctgaatcctc ttccacaccg catccagcaa
    15481 agaaaaaaac gtcgtctgta cttaacggaa tgaaaatcat gtcgatggaa actggtatgg
    15541 cctggattcg ctggcagggt agtacctggg ctgtcagaga aggtcagacg ctgggcaatg
    15601 tggtgatcca gcggattgat ccgactaccc ggaccatcat caccagtgca gggacgttac
    15661 gctgatgaac atggatgccc tgaccgccat tgagaacttt gccagcagta ttttcagtgc
    15721 cggaatggat ttcctgttta cctggggaga attcatcggg gtgatcagca tgattaccct
    15781 gtttgcccgc gcccgttcag ccggtccggt aaaaatgtct cccggaaaat ttattgcggg
    15841 gatgttaacc agctgcatgc tcgtatcact cccggcaatg attaacgccg gaggcgtaca
    15901 aatgggattc agggctgact catttggccc gattgcatat gtacaacctc agacgtttgg
    15961 cgctgctgct ggcgcagcaa atgcagttct gtcgctggcc aagctggccg gtgttggatt
    16021 tgtaatgaac gggatatcca tctggcgcaa ggccggactc gatggacata ccgcactgag
    16081 tgccagtgaa tccgtcagta agggaaacgt caaatttatt gccggcgtcc tgctggtctt
    16141 tatcgatcgt gtcctcaatg ccctgctggc aagcattgga atcgtcttct gatttttttc
    16201 accttcatat aaaccactga tatatccatt taaggagaaa ctatgtcacg tattcgtgtc
    16261 gtagcggccc gtctgtacgc cgcatttatc cacgcacaac tgtttgtaat gtcatgccgg
    16321 agcaaactga ctaagttcct gctgctcatt cccatgatgt tgctgcccaa agccgttctt
    16381 gccgatggag atcttgccga tatggttcgc aatgtcgaac aaggtgccaa aacagcacag
    16441 tcatcatcac tgactattgc gcagttcatc ggcgtaattc tgtttctcgg gggcctgatt
    16501 ggcctgaaaa aagttgggaa acaagggggg atgggactgg ctagttgcat tgtatctatc
    16561 gtcattgggg cggtacttgt cgccggacct gaaatgatga gtcgcagcca gaaacagttg
    16621 gggatttcct caatttccat tggctaatct tgtccagggc actggcattt atccggtgcc
    16681 cttcctgttc ggcaatacaa ggaggcacat atgtctgcct ggagcccgga gcacataacg
    16741 ttacgacgaa cagaaaacgg caatgtgatt gcccacgaca gtcgggacga tcaggaattc
    16801 atttttgcag aatgctgttc cgatgaaatc gcaaacatca ttctggaagc cgtgaaacgt
    16861 tacaccagta cggagcctgc tcatgcaaca cagcattaaa aacttgtggt tgtatccctt
    16921 tccagaaatt gatgtggttc atacacagga gccactgtta ccggaaccgg aattaacaac
    16981 gcccggtcga tgtatctgct gccggcaaaa cgtcaggcac cgtttcagac tggatgactc
    17041 atggccactc agacagctta ccgacacaat cagtgacaca cgagttcgtc ttaataaggc
    17101 cacagaacat ctggttaagt taatcaggcg tggtgaacca gtcgcaaccg gcgaaaagga
    17161 aaagtacaac acggcagtaa aagccgctga acgggcgctt gagcaggccc gtctgtctgc
    17221 cagacgtctt tcactccgcc atgtacagaa agctgaaatc accagtacag agccgttgtc
    17281 agagaaagag caggaactgt ttcatgaaga cggcccgcct tacagcttat gtgctttctg
    17341 ccacgcctgg cattcactga atggttatgc cgcagcacag ggcgttatgg tgtggctacc
    17401 cgaccttcat ccatccacag tcgtggccct taaccgccgt tcattacagg aagttttcag
    17461 taatgataag ttccgggtcc ggcggggacg ggaagcgctc tctgcactga tgcaaaacag
    17521 actggcggtt gaggataaat tccgcagttt ccgcccggcc gatttcgctg acgtttttcg
    17581 tcgctatcca ccttccggtc gttcgcccct gcgtgaaaaa atgaacggga ttgccctgat
    17641 tctgacacca gattcattca tcaaaaaaga atacgttgat tgatttttct tcatgccact
    17701 ccagcgcggg gtggcattaa aaaaatcaaa ctgcacaaac ctccctcctg taagtccgga
    17761 gttccctcac ctatgaacat aaaccgttta atcttcacta tcgaagactg tctgaacacg
    17821 ctatcccgtt tctctgtcgc aagcagtttt gttgaatatt gcgatttacg tactgtaatt
    17881 ggtctggaca gacaggatcg tgaacgccgc ccatggctga attcgcccta tattgcagcc
    17941 acaaagcgtg gagaatatct ctctgtcttt gaagtcagcg gtgctttcag ggaaatggat
    18001 gaagcatccg accagactgg cccaggttca ctggaatcgc tgatcaccag catgagtgac
    18061 agcctgaata cggcatataa aaacagcggt cataaaatca gcttcgtttt tgagcgtgat
    18121 cctgacatgg ggaaagaaga aatcgaagac atggtcgccc cacaaaagcg ctcactggct
    18181 aatacaggta tccagttaca ggatgtggtc gatgagaaag tcacaacact gtccccatgg
    18241 ctggtccggg agcgttgctg gctggcgatc tggagtggac ccgaccttat cagcaacagc
    18301 gatcgcaccg cccatgatga gcttgtcaga agactggctg aacgggtacc gaaagcacgc
    18361 tttgcccagt ccccctggca gtgggcctta tctgcactga aaatccgcca cgaagctttt
    18421 cttgataatg tggaacaggc actgagacat tcatctgatg gcctgattct ccggctactg
    18481 gatatacacg aggtcgggcg tgaaatacgt cgtcagacag aacggcacag cacaccgcgt
    18541 aactggcaac cccacctgcc tgaagatgcc cagccagcgg gatatcgctg gactgacgat
    18601 gaatccgtac tacatgctcc ttcattgcat ttgcagttat tcaacactca ggtgaccaca
    18661 cagggcaacc tggttcaggc gggcggactc tggcacggaa tggtatcgat tactttacct
    18721 ccgcaaaatc tgcagacgtt taacgaactg gtccgtgccg tcccccgcgc agtcccctgg
    18781 cgcatcagaa tggatctgat gcccggcggg atgaaggctc ttaatctgaa gaaaacgctt
    18841 ctgacttaca gcagcttcat ttctgccgtt cgtccaatgt atgaatccgt tatgaccctg
    18901 gctgcaacgg atgaaaaaga acctgtctgc ataatgacca tcatggcctc cacgtgggga
    18961 aaaacacgtg aaatatgcac acgaaaccag gccattctga aatcggccat tgaaggctgg
    19021 ggcgtatgtg gcaccacgac aacatttggc gatccccgac gcgcctgggt gaataccatt
    19081 ctcgcggcat ccggaggctc cggcccggtt cccctgtatc ccccgttgtc acatgccatc
    19141 tcgcttttcc cgcttaacag ggcaggctct gtctggcgag gcaaagggaa tctgatgtta
    19201 catacagaag acggttccgc atttgaggtt ggtctggcgt catcccaaca aaataaacat
    19261 acagaactgg caccaggcga tccgggactg ggtaaatcag ttctgattaa tacgttgtcg
    19321 gaaatacaga tatcatctgc tcagaaaaac ctgcccttca ttgcctatat cgataaggga
    19381 tacagtgctc agggactggt tcagcttatt cgcgacagtc ttcctccgga aagaaaagac
    19441 gaagctgtcg gtattattct gagcaatgat ccggaataca cccgtaacct ttttgacgtg
    19501 atgtacggcg ctaaaaagcc tattacgccg gaaaagaatt tcatgtcatc tgtgctgtgt
    19561 gctctctgtg tcgataccgg taccggtcaa ccctgtaacc ccggagacac ccgacaaatt
    19621 atcaaccagc ttatcgagct ggcttttaaa gagtacggtg aaaataatcc gcgactttat
    19681 cgcgcctcaa cggaagaact ggtggattca gcactgcagg actcgggact gtatgaaaaa
    19741 catgatgcag cctggtgggc cagatcaacc tggttcgaag tccgggatat gctccacaat
    19801 gccgggtata tcatggcggc tcagcgggca cattatcagg caatgcccca gcttcccgaa
    19861 gtgtcctcga tgctgggcca taccagtctc agggatgttt tcggtactgt tcagcgtgat
    19921 ggttcaaacg aacttctgct ggactacatc cgccgcgctc tcgagcaggg acataacgac
    19981 taccccatga tatccgggta tacccggttt atgatcaacc cggaaacccg ggtcatcgct
    20041 gtcgatctga ataatgttgc cggtgacaaa acgcctgcag gcaggcttaa aaccggcatc
    20101 atgtacctgc ttgcaggtca gatcgccggt ggtgacttca ccctgccaca atacagggat
    20161 gaagtgttaa agcagcttcc acgggaatac catgaaatcg ctcttaaacg tattaaccaa
    20221 cttgatcagg aggttaagac aaaagtttat gatgaactgc ataacgcccg gggcattgac
    20281 ttcatctggg aaaatctgga tacccaggag cgtgagcaac gcaagtttgc tatccgcacg
    20341 gttctgagta cgcagtatct gagggactac ccggaaagcg ttcttaagtc agcaaatact
    20401 ctctggttac tgcgctacaa acccgaagat atccccgtac tgagggataa cttcaacgtg
    20461 cctgaattta tgctgaaacg cttcctgaaa atgccggaag gcccggcacc agatggtagt
    20521 ggtgttcctg ttctgggagt attcagggta aaaagtggca cgcttgcgag aatactcaaa
    20581 tttaccgtcg gtccactgga gctatgggcg ctgaactcat cgccaaaaga cagtgccctg
    20641 cgtaaaacac tgacaaataa gttaggttca gttcgggcac gtaagattct ggccgaaaac
    20701 ttccccagag gttctgcaac gtcattaatt gagcaccggg cgggccaaca taacagtgac
    20761 aatgttattg aagaactggc ctcggagctc atccgaaaac agggatacaa tctctgatgt
    20821 gtacaattca tatcacccca cgctgccgga gacctgccag agggcaggta tttcttaaaa
    20881 aacttgaggt aaaaggtgac agtttctcat tcactctgcc gtcacgtgtc ttcaggttac
    20941 atccggcccc caatcccaga gtgatatacg cctcggatgc cagaacacag ggaaaggtta
    21001 caggcatgcg gctgttgttg ccgtccagca cagatgagct gataacaacg gcaatatggc
    21061 aggtttctgt cccggatgaa gcaccattcg acgttcagca tgtcatccag tggatactcc
    21121 cttcaccgct ggcttcgctg atgtccgatg atacctggtt atggccatcc gttcccggaa
    21181 cgccggcact gagtgccggt gactggcctg taatggatac tgacccttcc accttacggc
    21241 gcgtcagacg tcgttctttc agcatcatcg ataaccggaa caggaaaggt tatatcacca
    21301 ggcgatatca gtgtttccca ctggccccac tacctgaaga cagaaaatac gacagccttc
    21361 ctgaccttgt gctccacccc gagcaggaga caataaaatg caaagaaaaa cattactcgc
    21421 agcccttata gctatcctga atggaacggc ataccaggct catgcttatt cagtcaccgt
    21481 cgtcgccagc cgtcccgttg aacagcaggt cgtccccaga atggaggcca tcaaaggtgt
    21541 gctgagtgac atactgagta cccagacagc cacgggtacc gctatcaatc agaactctga
    21601 aaagctggca agtgtcattg cccagaacgg acaggcgaca cgtcagcaga tgattttcag
    21661 taatgaaact cagcgcctcg aggaggcacg gaaaagcttc accgtgccag actccatctg
    21721 cagtgaatcc gcatcaggga ttgcaacaga aagcaaaagt gcctcagcat ccgcagcctc
    21781 aaaactcagt aaaggtggcg gtgtgagcaa ccgtagcatc cgcgaccgtc tggccagcgc
    21841 agcaaactct cccgtgcggg aagcttatga tggcgcggcc atacacgcca gttactgtac
    21901 agaagcagaa tatgcacgtt ttggaggaac cgccgtttgt ccttctgtgg gcgaaatacc
    21961 aggtggagac agtcaggtac gctcgattta ccacggcgca ggtacagcgg acacacctgc
    22021 ggcactgaca tgggatcaga agcaaataga tgcagcgacg gcttacatga aaaacacttc
    22081 ccgcccctct gccggtcgtg ctctgggtaa aggagaagtg aatacccaga gtgggcgaac
    22141 ctacgtcgga ctccagaacg aatacaacgg catcattgat tctgcgtcga atccccaact
    22201 gacgttgatt gctgacagca cgcctaatga aagcacacgt aaagcactgg ctgaaacact
    22261 gcagtcagac tcagcggccg cttacttcga ccaggtggct tcaccagaag caaaagcacg
    22321 gggatatatg agtacccgtg agtttgaagc ttttgaggct ggccggcgct acgccaacac
    22381 agcatacctg gtggatttac aggaaatgca gggggataac ctgctccgtg aactggtaag
    22441 aatcactgcg caaatgaact ggcagctgaa cgatctgaaa gaacagatcc gccagggtaa
    22501 tgttatctct ggccagcaac tggctctgac tgcccgccag tattatgaaa aacaactcgg
    22561 tagccttgag aaaacaatca atcaggcaaa cgcacgttga aagctgacgt cagaacagga
    22621 ctgaaaaaat gaataagtta ccggaaaatt cagaccagac gaaaaacgtc aaaaaatcac
    22681 catccataac ccgcatagcc acccggggga ttattaacat ttttgttcct ctgatggaga
    22741 ctcgcctgat aacgaattct gtacagcata tgctggaaca acaaaaacgc aggctggaca
    22801 gatttcgtca ggtaaataat aaaaaggcgc aactccacct cacgtgggaa gaagctctgc
    22861 aggccagcca tatgtcagta gacgatctgg atcgtcgttt ccgccggcgc agaacagtat
    22921 ggcgattctg ttgctggagt ctgctggcca tcgctttgtt tctcagtggt atgctctttg
    22981 ccgcctcttc attacctctt acgactctgg tacgcgccat cagcactctt gtgctgatac
    23041 tgtcaggcgt cgctctttgt gcttccagag cactgattgt tacataccgc ctctggcagt
    23101 tgcatgaacg aaaagtcagc gaacccgagc aggggacttt tcgtgatttt ctgaactccc
    23161 gcaatggctg gcgcaatgcc acgttaattg ccgtaaccag taaacaatac tgatcacgtc
    23221 cagatgccag taccactggc atgtcgccat cccccttaat taaatcctgt gtggcctttg
    23281 cccatacgca attctattcc ggagttactg tgaaaatttt acttcgcgca ctctgtgcag
    23341 gccttgctat atcctcactg ccggcaatgg cttcagtgac ctatcaggat attgtgtctg
    23401 ccgcaaccaa tcctgacgat ctgtctcgcc aggcgctggt cacgatattc ggtgatgtag
    23461 tcaccaatcc actgtcaacc agcgccccca ctctgatcgg aagcatgttc ggggcgttca
    23521 acagtatcat cgccgtactt gccgttgtct ggtttatgtt cattggtatc cgtcatgttg
    23581 tgcgttcggg gcatcaggga caggtattca gcaccggacg cgatatcgtc ggcacactga
    23641 gtgtcgttgc aggttttctg atgatcgttc caaccggaaa tggctggagt atcgctcagc
    23701 tcattatgtt atggggcgca tcgataatgg gggttgggtc cgccaatgtg atggtgcaac
    23761 ttgccgctga taatatcgca aatggttatt caatgactgt acagccagtt caggcatcca
    23821 cacgaacagc agcgcgtggc atttttgaaa tggaactgtg taagtatgcg gttaacgcag
    23881 gactaaatga ttttaatcag acggccaaat cctcgacttc actgatgaca gaatcagcta
    23941 aaaccgccag tggtaattac actgtcacgg tatccaatgg cagcggtatc tgtggttcag
    24001 ccagcctgtc agtagaagga aacggaacaa cagatcaatc gacgattgga aaattcttta
    24061 atcccttcag taaaaacgaa tacagcggtg ttatctcagc acaacgtgca gccatggata
    24121 atatgattag cgacatggac aacgctgcca gtgagtttgt gacaacattt ctggaaaaaa
    24181 gaaattctgg caacggaaca ttgccggata tcgagacacg gattcagaga gcagccgacg
    24241 agtatgaacg tgctgtacaa aaatcgctac ccactgataa cggagagcaa tccagaaaag
    24301 aggctcttaa atcttatctc acaacctacg ggtgggtaac gcttggcgca tggtatcaga
    24361 cctttgccac tgctaaccag cgtctggcag agctggctga cagagctcca gctgtaactt
    24421 caatgtcatc actgggagaa gttggtgata ctgacttatt cagtgcggtc atgagtgcct
    24481 ataggacgca actgcagaat tcctcctata cgccaccgct gggcacagta gtatcatcca
    24541 gtgaacaacg catggcaaat gcacaagatc cgaaatctgc cttgtctgaa attatgggcc
    24601 cccttgttag tctcactaac aggattgcaa cggaaacaag tggcacaggc acaaccagtg
    24661 ctcaaattaa tcctctgata aaaatgaaaa atatcggaga tataacaatg gtaagtgcag
    24721 aaggaatctg gactatatat acaacggcca gagttgtcgt tgccggtggc aaagattcaa
    24781 ttcttgtaaa attctttaac tcactaacag gtgccgcttc gatgcttacc gcgctgtttg
    24841 aagcacttgc accacctgtt tattttcttt tgttcctgat gttctgtgct ggattttcct
    24901 tatcaattta cctgccattc attccgttta ttttctggat gacaggcatc gggaactgga
    24961 tagtcagcgt attaatagga tgtaccgccg ggccgctgtg ggctgcaaca catctcggga
    25021 catcagaaga cagaggaagt cgggcagcct acggttatat atacttaatt gatagtatga
    25081 taaggccgcc catcatggtc tttggttttt tctttgcttc tgtagccatc atcgccgtag
    25141 gcacaatact gaatgcatta tttggcgctg ccctggttaa cgtacagttc aattcgctga
    25201 ccggtatttt cagtcttgct ggtttcttgc tgatatacgc cagaatttgt acaaccactg
    25261 tagctgccat atttgccctt caggcctacc tacctgatca tgttattaac ttcctagggg
    25321 gacgtgatgg agccaacacg cttggcagca tggcaaattc tgttaaagaa atatttattg
    25381 gaggaagtcg caatatccga catactcctg gaatgaaaac agatcgtctt aaagataata
    25441 caaaaggaag tgacgacaag gacggaataa aaggataata tcaatataaa tattgcacaa
    25501 cctccccatc ggcattaaaa taatagatag tacgagggga ggcattatat gaaagctaaa
    25561 agagaaacaa cagacagatt tccaacctgg tggctatttt attatgtatt aagaaaagct
    25621 tatttctttc ttggaatccc tttttttctg ttctgcgcat taggctttac cgaaatgcta
    25681 tgttctgatc gttatttcgg aaataaggtc gaagattatg ttgtcacttt tggtagttgg
    25741 ttcttattac ttgcgccagg catatggatg tactcacgtg caaaaacaag gagagaaaaa
    25801 ataagaaaag ttgtacaaac aataaaagag tcaggcttct actctccaga gaaggggtat
    25861 gaaggattat ctttaactca gggagcttat ttcggtattg accttaaaaa tggcacaatg
    25921 ttgtatgtcc gtatttatcc aggcaacatt atggatgtta tcggttttga cattcataat
    25981 ttcactcgta ctgtaacaga cgacaaaaca ctggaaattc atacgaaata catcaacctg
    26041 ccaatggttc ctattccatc atggtgtact catccagaaa ccgcatcaaa caccatgcac
    26101 gcgatggcaa gtcgaggata tgattaccct gtcgactttc ctcgcctgat tcaggaaaag
    26161 cgtaaggagt gggagcagat tgcaggcgtg cctgtagccg aagtttttta agataagctc
    26221 cccccataac agccggttca aatgagccgg cttttgttca ttacaacaga taacttatgc
    26281 cgttaccgat aaattcgacc aagtgcttta atcgcagcaa tttttatttc catatcatca
    26341 ctttgactca atctctctaa ttcacgaatg atgttatccg cctggcatcg accttcacct
    26401 aatgcataga tcgcagcaag cgtaatgtct tttgatttac tcttcatcat ttcaaacaag
    26461 aaagtattag ctatcgcagg aggcatgctc ataatcaatc ctcttcatga aataaacaat
    26521 ggcactcact tacaaacagg agtccactat cgcaagcgac tcaacttagt aatatctctc
    26581 aatttgaatg aaaaaaccag atggcatgtg acagggaacc gtccgcatga tgtccgtgac
    26641 catttaaccc cataaactga taaaaaatga ataccggtat ctcaatgcga agaaataatc
    26701 tccgtagaat cccccatgag aaaaatatca tatgggagaa aatcgcaatt atcacagcca
    26761 gcgcattcat tattttcgct gtgttctggc attaattatt ttcaacacaa aaaggaaaaa
    26821 acaatgaatc tggtagacgc ctttgtcaaa aaagtaatat ccggtccata tgaagaatat
    26881 ggaaaatggt ggatagatgt tgaatacatt tcatggggtg tttccgggaa aactagactg
    26941 atgtttgact caaaggagca ggcactggaa gttaaggaag ggtacaaatt tttaacgtga
    27001 gatctctttt tgttaaaggg caggaagcgg taactccctg cccttagtta cgtttccagg
    27061 cttatcagtc gacaagttcc cggatcagtt ccagaacagc aatcagcgcg tttgcaacgg
    27121 caactactgc cctgagaacg atgattaaca gattaatcat ccgcttctcc atcactggag
    27181 gaaatcaccg ttgcttactc ttacattgcc tgtcacaacg gcactttcag tgttagttga
    27241 aaagagactt cctcctgcca gagcactgaa cacagcacca cctgtttgtt cagccaggac
    27301 ttaaaatctt gcatccatag tcccgatgga tatcacggca gaagtgtcgc cagacagagc
    27361 taacctgcgg attataagga tggttgctat caaatgaaca gctcgctatt ataagaacgt
    27421 tagttgaaaa gagatttcct ccggtcaggg ccacccactc tgtaacggaa cagtaaaaaa
    27481 accgacctca ccagtcggtt tttttacatc tgttgtttct caaaatccgc ccgacatgcc
    27541 cgtcacaccg gggaaactca tgctctgaat cagaaccaac catctcaact ttgccgtctc
    27601 ctgaaaaaac tggcacattc agttcgataa ctacccataa atacagagag aaaatccgat
    27661 gtcagaaaag tggagtaaag aattcgactc ttgctttcag gtggggagca tagaagaaat
    27721 tgcagatgct ctcgagaaac gcagcggaag cgaggaaaat gcgttagcca tgctggcaat
    27781 gacagccttc acactcatga cccgacgtgg tatctgcgaa atgcagaacg tggcccccga
    27841 tggcaaaggc attcggctgg agctaatcgg cttcagggaa aatgaaacaa taccggatac
    27901 gcttcattga agaagaaagg gcgcaatgag gtcagccttc gcaacaaagc ggcggcagaa
    27961 aacctgccgc cgtgttgccc gttaacgttt aacttcgtag gctaacgttg ccacaagcac
    28021 tgtgtttccc tgctggagcc ggagtccgca aagcgaatcc ctcaccatcc agacaaaaca
    28081 cagaatcgtc acacagatga cgattaacat cattaaaaac gttcgctgtg gcatcatagc
    28141 ccctccattg ccttacggcg gttatcgggc tactataatg ttgtctaggc attacagtag
    28201 agcccttggt tgattgataa aaaaatctcc aggggctttc tgctgtatat ggtttagtta
    28261 acaaagagag ccagtgttcc actgagcaaa accagtagct attcatgctt acaattatct
    28321 tctccatatc tgaaataact caaacagccc aatagaaaca tattgttaca tctaagatct
    28381 tgaaaagacc tctttgagaa atcaatatga gatcacggta tgttgatagg ataatatata
    28441 tgaagaaact attgattcga ttaatacctg atgcaatata tgaggctctg gaaaagacag
    28501 ctctgcattc tgaacgctcc ctggaagcac aggccagata tatccttagt tgctccgtag
    28561 ataatgaaaa acaattaact ggtggtgaac gctatcaaag agaaataacc gcgcgtctta
    28621 atcaagcgtt atcagaagct aatgaggtca taacagccat taatcttgtt cccgcaagaa
    28681 ttgcagaaca attaggccat catgacgcca tagaatccga aaattggttc accggaaatg
    28741 ctgttcctag ctttacagag cttgacgaat taagcgatat ctttggatgc tctccagatt
    28801 ggttaaagtt tggcgaaaat gtaccttatc caaagagttc taaaggaagg ataaactgga
    28861 atcgtggcgg agaaaaagat atcgatgcac ttttagagcc tgacaacaaa ggtcgtaaag
    28921 tctcatctat tcatattttc agggtaaatg aatctggcaa tatactaata ctgagagaat
    28981 ttgaaaacag cataactaca gattttttct caacaaacct ttacttatca gacaaagaaa
    29041 aaataggaca gggcggattc cacgacctgg tcgatttcct tgtaatttta caatcccttt
    29101 atttaaaata tataaatagc aatctgttag taaaaagcta taacattagc caatatgtac
    29161 tggattcctg ttataagagc ggagaaaagc accccataac tatatgtagt gcaggtgaga
    29221 caagtacctg gtgggaagac atatggcatg aagacatgat agctcaatcg aggaataatg
    29281 agtcctactt ctgggatggt gacaaacctc ttattgatag ccttcataaa gaacttcaaa
    29341 aaaataacag attaaagcca aacagtgagt tagatatgcc actaattggt taaacaccaa
    29401 ttaaagatta tctagctaaa acaacatcgc acacgcatac tgcaaagccc ttggtgagtt
    29461 aatagaagaa ttactaaggg cttataaaaa atatataccc ccacattggt gacacattaa
    29521 aatatttttt gttgatgtgt taccagcctg acccaaaaac aatttttaat ctttccgttc
    29581 gatttaaatt ccagaaatgg agactttgtc atgtcttaca atcgacagcc tgtcgcagaa
    29641 gatcctatgc agatatgggg cgctgttggt gtccttctta tcctgctgct attcgtcata
    29701 tggttattct tgccggaagt ggtttacacc agctgcctga ttcttcatac cttatgggga
    29761 ctggtcgact ggggaccatt tcataactat gcagcaccac gatacaacct gcttgccatg
    29821 accggtaaca acgccgccaa cataagctat agccagtggg ttaacgtcat ggaacagacc
    29881 ataggtattc tgtggatgta cctcttacct gtgacactct ggtgtttgtg ggaatggtat
    29941 caacatccgg gacaaagccg tttcacccgc agaccagtag atattacccg actgccacac
    30001 atctttgctt cgttatcccc tgctatcgcc cctgtactgg cagatggtga tccgaaaaaa
    30061 ctctttcatg gtggtaaacg acctgaacgg cgggttgctc tcaccccgga agcatttgtc
    30121 gaacagcata ctctcatcac caatatgcag ctcgatgttg cagctgcaag acgatgtttc
    30181 atggcccagt tgggtaaacc tttaacgtcc tggaaagaca tggcaccaca tgaaaaagcg
    30241 ctctttgcca tatttggcct tcagtatttc ctggatgatc ggaaggcagc attaaagctc
    30301 atggatacac tgaacctctc atgcagaatt aaaagcaaac gggacagcgg gaaattctgt
    30361 acccctgttt attcactggc caaatcagct ttccagagag tgattaaaag caacggagca
    30421 cagcaatggc tgaaacaaca tcgctacgtc cgcagtgggc tggtctggct gtatgcacat
    30481 gacctgcggc ttacgccacc caactggatt tggctgaaag gcgtggacag aacgctgttt
    30541 tatgcattac accgcgccaa taccacgaaa ggatttattg aaggcgctgg cgtggtggcc
    30601 gttgcacgtg cagaggccga agccatgcgt tttggtcttc cctgccctga accctgtgtt
    30661 gatgaagcgg tggaaggctt acggcgggat atgctcagtc tcggtcttat ctgggatgaa
    30721 ccacaaccag atcgcgaccg gaaacgccgc atcctgacca actggtccct caccgacgac
    30781 atcctgcccc gcacacctgc tacagacaac gaattctgat tatccggaga actgaaaatg
    30841 acaatcgagt actttgcaac acgcattgca aaacatattt ccgtgcaggc aatccggccg
    30901 gatggcagga cagaaattat tgccgtcctt ccacgggatg ccctgtcagg gctggtaaca
    30961 cgaaataatc aactgattca tatagccttt gacgccatgg gcacccgtaa cagagaaacc
    31021 gtcctgattg ataaacagga gcataacgct gaccagatac ttacagctat tgattcctgc
    31081 ctgcgccttg aatatctgat ggaacgccgc ttcagcagaa cacctttgtt ccgtggcata
    31141 atcgccagcg ttgtgctgtt cgtaatggca acgattggct tctctctgtt ccgctatgtt
    31201 gatcgtgtat tctgggatga tacaacgcct gaagcagtac agacagcagg tgagccacga
    31261 ctgctacccc ctcacctgaa tcacactgtg ccattaaatg aaggcattca actcccggtt
    31321 cccccgaaaa aagacgtcca ggtcctggag aaaacaatca caagcaaaaa tccggaagcc
    31381 gcagctgcgc gacataatct ggctactgtt ctgaagcgga acgccgatcg gggcatgttt
    31441 accatcaacc tgtcatccgg tcatgaacga accctgttcg catttctcga tcctgcctgt
    31501 cctaactgca gacttctgga gccggcctta aaacgcctgg ccagcgactt caatgtcgtt
    31561 atctacccgg tttcagttat tggtggagag gaatccaccg accgtgtggc accactgctg
    31621 tgtgaaaaag atgctcagaa acgtgctgcc ggctggcacc gactgtattc cgcagataac
    31681 ggaatgatga cgccatcaga agaaacaacg cctgcagatg aaacatgcct gaaagcagcc
    31741 agagctgcga ttgatgttaa caatgtcgcc ttccgtaagt tcggatttgc cggtacccca
    31801 tgggtgctgt ccgacaccgg ctggcatctg cctacaggaa ttctgcagga aactggcacg
    31861 cttaatttat tcctgaaaac caccgacagc gagtctggcc atgagtgaac atcgcgttaa
    31921 ccccgagctt ttacaccgta cagcctgggg aaatcctgtc tggaatgcac tccagagtct
    31981 caatatctat ggcttttgcc tggtggcaag tcttgtggcc agttttatct ggccacttgc
    32041 attaccggca tgtctgttgt tcacactgat aacaatgctg gtatttagtc tgcagcgatg
    32101 gcgctgccct cttcgtatgc ccatgacgct ggaatgtgct gacccgtcac aggacagaat
    32161 gattaaaaga agtcttttca gcttctggcc aaccctgttc cagtatgagg tcattctgga
    32221 atcaccggcc agcggcatat tctatgttgg ttatcaacgc gtcagggata ttggcaggga
    32281 gttatggctg agtatggacg atctgacccg ccatataatg tttttcgcca ccaccggtgg
    32341 cggtaaaacg gaaaccatct ttgcctgggc gattaaccca ctttgctggg caagaggatt
    32401 cacccttgtc gatggcaagg cacagaatga tacggcaaga acgatctggt atctggccag
    32461 acgctttgga cgtgaagacg atgtggaagt cattaacttc atgaacggag gaaaatcccg
    32521 cagtgaaatc attctgagtg gtgaaaaaac gcgtcctcag tcaaatacct ggaatccctt
    32581 ctgttacagc actgaggcat tcacagcgga gacaatgcag tcaatgctgc cccagaacgt
    32641 ccaggggggc gagtggcagt cccgggccat tgccatgaat aaggcactgg tatttggtac
    32701 taaattctgg tgtgtgcgcg aaggcaaaac aatgtcgctg cagatgctcc gtgaacatat
    32761 gactctcgaa gggatggcca aactttactg tcgtggcctc gatgatcagt ggccggaaga
    32821 agctatcgca ccgttacgta actaccttca ggacgttccc ggtttcgacc tgtcgctggt
    32881 caggacacca tcagcctgga cggaagaacc cagaaaacaa catgcttatc tctccgggca
    32941 gttttctgaa actttcagca cattcacgga agcattcggg gatatttttg ctgaagattc
    33001 cggtgatatc gatatccgtg acagcattca cagcgacagg attctgatgg taatgatccc
    33061 cgctctggat acatcagcgc atacaacgtc tgctcttgga cgaatgttta tcacccagaa
    33121 aagtatgatt ctcgcccgtg atttgggcta ccgcctcgaa ggaactgatt ccgatgcact
    33181 ggaagtcaaa aaatataagg ggcgtttccc ttatctgtgt ttccttgatg aagtgggggc
    33241 atactatacg gaccgtattg ctgtggaagc cactcaggtg cgttcactgg attttgctct
    33301 gattttgatg gcacaggacc aggaacgtat agaaggacag acaacagcca cgaatacggc
    33361 aacactgatg cagaacacgg ggacaaaatt tgccggcagg attgtcagcg agggcagtac
    33421 ggccaaaacg ctgaaaagcg ccgccggtga agaagccagg gcgaggatga acaacctgca
    33481 gcgtcaggac ggcatatttg gtgaatcatg gattgacagc ccacaaatca gcatcctgat
    33541 ggaaagtaag atagatgttc aggagttgat tgaacttcat cctggcgagt tcttttctat
    33601 tttccgggga gaaaccgtac catcagcatc attcttcatc cctgacaatg agaaaagctg
    33661 cagtagcgat cccgttgtta taaacaggta tatcagcgtg gatgcacctc gcctggaccg
    33721 tctccgccgt ctggttcccc gtacagctca acgtcgaata ccgtcaccgg aaaatgtcag
    33781 tgccatcatt ggcgtcctga ctgcaaaacc ctccagaaaa agacgaaaaa ttcgcacaga
    33841 gccgcacacg atcgtggata cattccagca acgcattgca ggacgtcagg cggctatggc
    33901 aatgctggaa gagtacgaca cagatatcaa cgcacgtgaa agtgccttgt gggaaacagc
    33961 ggtgaatacg cttaaaacga ccacacggga agagcgacga attcgctata tcacgctgaa
    34021 cagaccagaa ctacctgaaa caaaagagga aaatcagata tcagtgagag cagaaagagc
    34081 aggtataaac ctgttaacgc ttccacagga caataaccac ccgacagggc gtcctgtaaa
    34141 cgggtttcat cataaaaaaa ccaaccgtcc ggactgggac ggaatgtact gaacagtggc
    34201 cgcgccggat tctggcgcgg cctttttttt aacctggaga aggcggttcc cagcgtttat
    34261 cctgctccgg cggaggcagt gatgcgtcat cgttgctgtt cgccgcgata taatcgcggt
    34321 agtagcgttc acttccttca cggtgatgat ccacgtccgg tcgcgtaatc ctgtagtctt
    34381 cgtgaccggt acggcctgtc acattatgcc aggcaaccat ttcagcaaag acctggttaa
    34441 attgtttgtc gtttccttct ggttcaaaac ccatgcgtgg atcacgcccg aataataccg
    34501 gtgcaatcaa atccagttta tctgccagcg cattgcggtc atggtgatta cggaaaacaa
    34561 ccctgtcacc ataatccgta cagacaaact ctcccgtccg acggtcacgg aaacgaacac
    34621 tcccgttctt ctgcagacgg gcctcgagat caccggtatt accgtatacc ggcgttccgc
    34681 ccggttcaca aagcacgaca cagcggtctg tcgtcgttgt gcgtgacctg tctttacggc
    34741 gatcgcgata atcccagcct ctcagctgcg atacagcagc cctgtctccc tgagcagcct
    34801 gaatttcgac ccactgacgg taagagggtg gataccactt cccgtcagca ataagtttct
    34861 gtcgctcatc cctgatgtct tctttcagcc tgatcaatgc ctgcattcgc tgaacttctg
    34921 caatgtgata atgcagctta cgtaatgccg gatcatcgta ctgcgcacga atgtgtgact
    34981 tccgcagacg gcatgcctga tgaatttccc gacaacgttc cccataacgc agatctggtt
    35041 tacgccattg ttcacgccat gccagatagc gggcacgcag gtcagcacgg gcctcagctc
    35101 tggcttcacg tcgctgccgg cgcagcattg ggtcacgttt actgctgacc ccgtacctgt
    35161 cactgacagc cagctccggg ttatagcgac tttcaggttg caccctgtca aacaaatctg
    35221 ctggggcact cacaaaaggt ccggcctgtg gctctgcacg ccccagtgtc agatcaggat
    35281 gaatgctgct ggccttcacc ggcgtttgct catgattaaa ggcatcaacg acaacaagcc
    35341 cgtgatgttg tttctgtagc atcagtccct gttgtgcaaa cagctcatga caacgctgcc
    35401 aggtaaactc accctgtgtg atcatttctt caatctgagg gctacaatgg ctgatgctgt
    35461 atccccacag actttctttg tcagaaaaca gttcgcgttt gatagccccc tgcggccccg
    35521 tgcctgtacg catacggtgc cattctttat cggtcgcagg acagctctcc atacgacgac
    35581 cacggaagcg ttctgttggc gtaacctggc ggaaaatatc tgttggtaca ggctgccagc
    35641 caccatcgag ttgattcaca ttgtcgagcg tcagcagcga ccatacgctg tcagcacgaa
    35701 cgggagtctg gttatgatca taaccgtcac agataaccag gtgaccatgc tgaacacgca
    35761 tccataatcc cgcttcagcc agtgtgcggt gaatagcctg gcagttttcc agccgtcctt
    35821 cccgtgccat ttccggcaga cgttctccaa gatgacgaca ggcatactgc tgcagacttt
    35881 ctgtttcagc gattttttcc gggcgaacat cagcggcaat gaagtccgga ttatagcgtc
    35941 ctggtgcttc cacctggctg aaaatgtctt ttggtactgg cgtgtagtca cccatttttt
    36001 tcatgagttt ttctgcacac caggagggac caaacgagtc aagctgtaca ccttccctgt
    36061 tgcgatccca gccatccatc accagaaatt ttccgtccat ctgagacagg tacagaccat
    36121 cttccgcaag acgacgatgc agggataacc agtcatttac aggttcactg cgtaaaccag
    36181 cgaccgctgt ctgcgcgaca tactcgcgaa aagtttgttt ttttccgcgt gtccaagcgt
    36241 tctggcgatc acgttcaacg gcagttttgc gaacgatacg atttcccggc gcatggaccc
    36301 agcaaccgtt atccggggca aaaccatgct tcagttcaag ctcacggcag gcacggctga
    36361 gtttttcctg actccatgaa aggcaattga gataacctgt aaccggatgt acccggttga
    36421 ccgcaacgtg aacatgcagg ttatcagtat cggtatgtac ggcagaaaca tactgatgtt
    36481 cacccagccc gagggatttc agggtatggc gtacactgtc ataaatctgt tccggacgcg
    36541 ggctttcatg ggcctgccag gaaagaatgt agtgaaaaac aggatcagta ttatctttcg
    36601 catagtgtgc ctgctgtgca atgtactcca tatctgcagc agcagtttca agcgatgtac
    36661 agttgtgaaa acaggtcaca ccgtaaaagt taacccattc gcagccgtcc ttcatgacat
    36721 caaccagtgc cacgaatgac tcatttcgaa ggcgtgtcgc ataatcaaca agacgactga
    36781 aacggctccg gtgcggttgt tcagcctgcg aagaagaaga taaattcagt tcctcatcgg
    36841 tcatgtcatc ccggaccgag acgtaggaca ccaggtcttc gaacgaagac ttgccgtccc
    36901 ttctcttttt cggaatgact gcattcaccg gagaatcctc aaccttccat taactgttta
    36961 cgcaatgtat ttgtaagttc ggttatggca acaagcactt ctgcgtattc cttgtcaccg
    37021 gttctttttc cctgaacaaa cagatgcttc tgcatccttc ccagtctcat taactccttc
    37081 agaaaagcat catctgtacg ggagttaatt cgcttattca gtgctgcatt tcggatgtaa
    37141 gcagatacag taagtccgga atcttcagct tttttcctga tggtttcatc ctcaacagga
    37201 gagaatctga gtgtcctgac aactgtcttt tgccgtacct cacttttctt tcttaccgca
    37261 ctatcactca cttcaggctc cttacggggt gtcggggcga agccctgacc agatggtaat
    37321 tgtaatagcg tcgcgtgtga cggtattaca attacacatc ctgtcccgtt tttcaggcca
    37381 ttatagcctg ttatttttgt gcatcatagt cttatgcaga cggcagaagc agggcgcagc
    37441 cctgccagga ggcagttttt gatgataaaa tcagacttca acatgaaacg gagagaaagt
    37501 gccaatggct tctaaaaaat tttactcaga tgatgacatc cagctggcaa aagctgcctt
    37561 atcggaattg ccggacctca ccgctcaacg gaaaacattg cgtgattttc ttgatgccat
    37621 tcgtgatgac atcattgtcc tggtaaggac aaagggttat acactggcag atgttcgtga
    37681 cacgcttaaa aatgcagggt atgaagtagg agagaaggca cttcgtgaca ttattcggga
    37741 agcagaatcg aaaaaaccat cccgccgttc ttcctctaaa acagcaccta aaaagacaga
    37801 ttcaggcagg aaagatggca tcgatatgaa caacaattga tgtgacaatg tcacatcgtg
    37861 ttctgagatt atgatgatta cagaaaaaaa agccccggca taaataccag ggcttttctg
    37921 tttaatcaga ctcgtggcgg caatgcacca ggataatgcc gtgcaataat atcattcagt
    37981 ttttcaacaa ggtcaggacg cttaaagaca atgtgtacgg tccctttctg gtagccacgc
    38041 accgtaaaat attccccttc taacagctta ccgacgttgt cgccgttccc gagaaattca
    38101 ccatactgcg ccccgatgct gctccggaag tcaggaacgt tgcgcccctc cagcagataa
    38161 aaaggacgta ccaggtcatc cagtgcctcc cgtccgctgc agtccagcgt cacacgcccg
    38221 ttactccagc ggtacagcat gttttcaata atgattctct tgcccagacg gcagggatta
    38281 ttggttctgt aatcccatga caatttccgg tagacatcaa tcagtccctg ttcaaacgtc
    38341 tgcatcttgc tggtattaag atggcggaat gttgccagta cattgtccag ggtgatttca
    38401 ggacaggtgt cgctcatcag ctggctgttc cactcatcac gctggcatga actcatgaag
    38461 gtgtacatgc ccgtatctgt catcaggcgg tcccagattt tccggtcggc aagacgggtg
    38521 atattccttt tcgcttcttc cggcttatcc atgcgacgaa cggaatcagt cacgcagtct
    38581 tcaaacccgt aaagaaaaat atcacctgac gcatcgagca gaatcctgcg cgctgtcgcc
    38641 aggcattcca tcgcctgcat gaatagtgca ataccttcgt tacgctgcgc gatgatgcgg
    38701 tcgatagcca cagacggaat aacatctgtc gtgccggcag tattcaggtc agcagtgggt
    38761 aaagtctgat tcatcacatt gtctcctcat cgtcacgact gccgggtcag ccaggtcatt
    38821 tcccggagga gcgaagctcc ttctgccgtc cgggtcttag ggtatttccc cgcagtggac
    38881 ctttccgacg ggggacgctc accacgtcac ggggcagttg tccgggggca gacgcaaaag
    38941 tttttgacgc cagcggcaaa aagttttgtg gctggtacat ctctcccctg gacgactgaa
    39001 acgggatgtg gtcgtgtatg tacccccgga aagtccgtct gcggagaaca ggccgcagtg
    39061 cggcggcaga caggcaagag acagctgcgc catcaggcgc agtggcctta cacaccgtaa
    39121 ggtgtgcagg gagcgccgca tccggcgcga ctgaagaaaa acgacggcac aacaatggca
    39181 tgtggtggtc actggcacac cactcccgca caggtgaaaa cagagaaaat cgcacgacat
    39241 ttacaaacaa aaaaagtgaa atttcttcac ttttttacac ttatttatcg cgctgatttg
    39301 gcaattgtga tgataaaata aagtgaagtt ttttcacttt ttcaaaggtg ttttatgacc
    39361 ggctgggaac tgcgaatctg gcgaaaaagc atgctatggt cacgcgaaaa agcggccaga
    39421 gaatttggcg tgacgcagag aacatggcac gcctgggaaa atgcggaaca ggtggacgtc
    39481 accgtctgga ggaccaccca ggcactttca gtccgtgacc tgctgcctca catgcagggg
    39541 atgcgcaagg cagatatcat caggcgcctg gaaaatgaac tgggggaaac ggcagggaat
    39601 gtgtgagcgt gattcacagc ctggcagaat gtcaggctac tgctcatcag gaaacagctt
    39661 tttcagttct tcaccggaaa gtccggtcgc ctcctgaatc cgttccgacg tccagcccat
    39721 atcccgcagc cggcaggctg cttcccgcgc cacttcaagc gcacctttct taaaaccttc
    39781 tttaaaacct tcgtcgaaac ctttctgtat aagacgttct gcaagcgtca tactgttttc
    39841 tctgctcctg ctgacaaaaa agcccggcat tttgccgggc tgtgatgctc tcatcagtga
    39901 atgatttttt tcagttcagc ttcggaaagt ccggtagcct gcttaatctg ctccggcgtc
    39961 atccccattt tctgaagctg gcacgcaata gccagggctt tttcaagcgc tccttcctgg
    40021 cgaccttttt gctcaagacg ttctgcgata gtcatcagtt tatcctcatg ctgcggcaga
    40081 cgctcagcca gcgcccgcat aaattccggt gatacagagt cccctgcgtt aacgatgtaa
    40141 tgaatcagaa cacgcacctg ctcgtctgaa atttccacca tgacctgcgg gagtttgtcc
    40201 agcagctcca tcatgtcacg atggcggata tgcttcatca gcagcgtcag tgcggccatc
    40261 cggcgatggt tcatgatttc attgtcatca acgacagtga tatcaaccag cggaaacggc
    40321 tttgtgtata ttttagccgc aagtgccgga ttctcaaaac agtccagcca gttcatgctg
    40381 tacgggtacg ggctgcgctc accatgatag aacagcacag gaatcaccag tggcaacgtt
    40441 ttgtgctgct ccagatgtct ctgcatcgcc gccacactgt aacgtaacat gcggaaaggc
    40501 atgtacagat tttcggtgct ctggtgctcg atgagcgtat aaacatagcc gtcttcaccc
    40561 ccggtggttt tcacactcca gaggatatcg ctggcgtact gatgcaggtc cggctcaaca
    40621 aaggtggcgg gttccagctt cagcgtggac aggtcgcaca actgccggta ctccgccgga
    40681 agatgcagtt ccagaaaatc tctggcgacg tcgggattcg ccaggaacga ccggaacgcg
    40741 gcatcatgcg gcgtgggcgt cgtggtggtt ttcttcttac tcacggttcc tcctggtgag
    40801 atgtctgaac gtatcctgcc gcctgcacag gcagacggca agggttaatc agtggcagac
    40861 ccgctcaatt tcaagcgcgc ggagcaggtg gccccattcg ctgactttca tagcggggat
    40921 acgtacccgc ttctgctcac tgcagcgtct ggcgatatga ccgctcatcg ccaggacgtc
    40981 cgggttaatc tcatcctgtg ccagctcctg acgtctgaag cgggtatggc ggtgcagggg
    41041 tttattctcc cgcaggcact cttcacgcag acgctttaca tactcggcgg ttgcgaagat
    41101 aattgatggt ctcatggcat aatctccata gatagtgatt gagatgccgg acttcctggc
    41161 ggggaagcgc cggcggagag aggcagggtt tatcgccctg ccttttttca tttcggacag
    41221 cgacgcagtg ccagcgcgag cgctttcatc ttccagcctt ccggcgtgcg gtacatcacc
    41281 ttccagtagc cgtcgatgaa cacataatgg aagcgcacct cttccgtgct ttcagtggca
    41341 atagcctccc tgaaagaacg gtactcacgc gcctcactgt ccatccggaa accactgtca
    41401 cgtccgtaat aagccgtcac accgtccacc gggttctgaa gcgtatgccc ggcgggtttg
    41461 tcgcaacggg gaccgaggca acggatatca ccgtgttcaa ccagggcttc ggcctgctga
    41521 ccggtggcat agtgggtatg caggatgcga ccgttccagg tcggctcacc gtgcaggtgg
    41581 cagtaaacgg tacggaacac cccgtcgcgg caaaaaacgg aaatcgtgct gctgacagtc
    41641 atgaaaacgt ctccttctgc tgaatcaggt cgccgggccg accggttgct ccccggaggg
    41701 gcgaaagccc cttctgccgt ccacttcttc ggggtttctc cccgcagtgg acctttccga
    41761 tggggcgtcc gtcaccacgt cacggggcag ctgtccgggg gcggacgcaa aattttttgc
    41821 cgggtttatg gctaaaaatt ttgtggacgg tatatcttcc cctggactgc tgaaacggga
    41881 tgtggttact ccagcccccg gaaagtccac agcgggaaac aggccgcagg ggtggcggcg
    41941 gaagggttga ctgcgccatc aggcgcagtg gccttacaca ccgtcaggtg tgcagggagc
    42001 gcaggtcacg gcgcgacaga ggcggcgcga ccggaacggt cgctgcaagc ctggtttttc
    42061 agacatcgga ccgtagcggc agcccgaaac cgcgtcagcg ggttcggcgg agactggcgg
    42121 gtgcgccagc agctgcctgt cggagtcggg cctgacgacc ggatgccgcc aggcagccgg
    42181 gagccgaagg tgtccccgcc tccggaaatc agaacaacag aaacgacata acagaaaaat
    42241 gcctcacacc atgacgggca ggaagggcag cgccgcaggc gcggccctcc tgcatttcag
    42301 gcaggcgtaa gcctgccagg gttttccggc ggcctggcag gccatacccc cgccggagcg
    42361 ggggagtaaa tcaccgacgg aacacatgac cgtcaataaa ggtgaaggag tccaggaata
    42421 aatcccgcgc atacgcctca tagtcaaaat agagcgccac cgtctccggc acgtcagcca
    42481 gcaggccggt atcactggcg aactcaaccg caaaagcctc ctcactgtca gcctcgcccc
    42541 accaggcatc gcggaaggtg tcaaaatccg tctcaccggt atcctccacc cagagacgat
    42601 aagcctcttc gcagccttca tcccgtgcct ggcggaagcc ttcaacccag gcccagttga
    42661 tatggcattc agaggccata ttccccggga atccctcata atcctgaaac atcagttcag
    42721 gatcggcttc atcctggtga agagcacggc aggcggcgaa aaagtcgcgc tcatcatcaa
    42781 acgtggtcag gtcaaaccag cgtccggcga tgcttccaca gttgtatttg tgccaggttc
    42841 caacgtatac agcaggtgca acaacagaca ttcccgaatc ctccatagtc acatgttctg
    42901 cggcaccggg cggtgccgtg cctccataaa agccgggcga taaggcgggg atgcacaggg
    42961 cgcaagggaa gccgcagcct gacgcacacg gctggcggga gttgcagggt gaggccgcga
    43021 aacctgagcg ggcgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc
    43081 gcagcggcga agcgtaccct tgcatcaacg ccagcccggc tacgatggag gcacggcaca
    43141 gcccgcacac gatgctgagg ccacatagct gaaaacccac aaggccatgc cccgcatggc
    43201 cgggtaaaag cggagccgcc ggcgacttcc ttattccggc gagtgcagag gacacgccgc
    43261 atgacctcca gaccacccgt gactgctccc cgccctgtcg ggcgaggctt cccacttctc
    43321 aggccgcaac cgtctgtgcg acggatttac gctggcctcc atgggcagag acgacgagtc
    43381 ccgccgcctt tagttccagt attccttttt gccggatgtt gagtgctgca ttgatatcgc
    43441 ggtcgtgctc tgctccacac tcatggcatc gccagatgcg tttatgaagt ggcatttccg
    43501 gcattttgta accacaacaa tggcacgttt tcgaactggc aaaccactgg tcgagtttta
    43561 ccaggtgaac gccttttgtc gctgtcttgt actccagctt tgtgatgaag ctgtgccagc
    43621 ctgcatcacc tatcgctctt gccagattgt ggtttttcat catgttggct gttttcagcg
    43681 tctccacaat taccgcttgg ttttcgtcaa caattgcacg agagagcttg tgttgaaaat
    43741 cagcacgggc attcgccacc cgttcgtgta aggccgcaag acggattctg gcctttttac
    43801 gattcgcgct tcccttttgc ttacgggata atgctttttg tttgcgtcgc agattacgac
    43861 ctgcattgat gagatggcga ggattcggaa cttttacagc atcagaagtg atggcatagt
    43921 gctccagtcc catatccaga cctgtaatct tttcttcctt caggtacgtg ggtttctctg
    43981 gtgcggaagc gccatcgtca caaagaatgg aagcgtagta tttccccgtt gcagaacggg
    44041 tgacagtgat gcttttcagc tttccatgaa gttcacgatg aaggcgtgct tctatcgggg
    44101 ccagtttggg gattttgatg gcattatcct gcaccttaat cccgacacag tgatagctcg
    44161 attgttttcc gtgtttgcgt ttaaacgtcg ggaaccgggc tttcagcttc ggattaaaaa
    44221 agttggaaaa tgccacatca agattgatta ccgcctgttg caatgccatt gagtcaaatt
    44281 ctttgagcca ggcatatctg cgggacttct tcgccacaga caacaacggt tttaaatctt
    44341 tacgcggact taaattcact ccgtgtcgct ggtaagcgtg ttttttgatg tgaagagctt
    44401 tgttgtacgc aaaacgtacc gcaccgaact gggcgttgag aaatgcagcc tgttcaggag
    44461 taggatagat gcgtattttt gttgctctta acatagtcac cgctcattgg tacaggtttt
    44521 tatgctaaca ccgtaataat atttttaaag cccttcgggc ttttccgcct ttcctcccca
    44581 cccgcattgg gcgagggttc tcggcggctt ttcgctgatt tcatcatatt gcgttctcca
    44641 gcttgttcgg gatcatccac tgctcaagtg cacgtacctg taccggtgca ccacgaccgg
    44701 cctcaccggc ttcaaaaatc agccgccaga gtggttcctc ctgccagaaa aaccggtcaa
    44761 atgatgacag tgatggaaaa cgcccgcacc acccccagaa aatcgtttcc agatcatgct
    44821 cctcaacaac accagaccag cgtgacaacc agcttccgac cgtggcggga gtatgaaacg
    44881 caaggtcaat ctgcgcctga gcaaaacatg tctggtattc cagttcccgt cgttctgcct
    44941 cacgttcccg ctggcggctg tatttctgct ctgtcagcgt ggttttctga cgacgccgct
    45001 caccttcacg aaaaggaacg gacgggaaca gacgatgccc cgcatcggat ggcaggggaa
    45061 gaacacagac ttcaccaaaa ctttcaatca gtttatccac ggcagccagc cacaggagac
    45121 ggttgccatg aaattcctcc ttcgtcagcg caggagcaaa aaagcggaga tcggtcagcg
    45181 aaatccggcg actgccattc aggcagcgaa agaaagcgct ggcataaggc catgctgaaa
    45241 gtgtgcgtcc ctgacgacga cgttgttcgg tgacagccac agcctgcatg gcggcctgtt
    45301 gttctgcgct gagggggatc agtgcccgtg aacgtgcgct catgcctgac ctccttctgc
    45361 catcaaaata tgaatgatgt cgttaacact gtatccctgc gcatcaagtg tcgcggcgag
    45421 cgccagcgtg tgactgatcg cgacaggcgt gaagatatgt tgcacctgaa cgacggcgaa
    45481 aggctgtcca ccaccattca ccagaaccag catccagagt tgagagacgt ctcccggcga
    45541 acataatgcc aggtggaaac gttcgtgggc tggcgtgaaa cgaacctgga cggggaaaaa
    45601 gcccccgaac tcactgccat actcgccatt catcatccag ttagccggtg cgtccacttc
    45661 acgcatcacc tcgtgagtga gattacggcg catatcgctt cccgcagcac ggatatcttc
    45721 atattcctgt gcgttcatgg actgcagggc attcagggtc agttcagttt tcatcacggt
    45781 atctcccttg ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc aggcggcatc
    45841 agccaggtta gtggtgtgtt cagtggcatc catctgtgga cgtggtgcac acatccagcc
    45901 aggcacccag cggttgtctt tcatatggaa ctctgcatgt tcagccgcat cgcctttctt
    45961 catcttctcc gcgtcccgtg cggcaccgga cagtcctgcc tcattcaggg ctgcgataat
    46021 ctgcggcttt ttcaggtgtc cgaagaagtt tgcttttgtc ggctgccacc agtcgcgcat
    46081 gtgaaaaccg atggcgctct ccagcgtgtc cagcgggctg cgtgacgtgt gaccacactc
    46141 gcgggtctga acgccgtgga tgctgcacgc ggtgcagaaa ctgagcaggg ataacagcac
    46201 ctcctggctg agcgacagga acgtcgtcat gtcccgtgac catccctccg gtaacagggc
    46261 agcaagacgg gctttttctg ccatcagcgc catgaacgcg gcaccttcct tccctgacgg
    46321 cgcatcgctg gtcagcgaat aatgtttaca ttccaggctg atttgtgctg gtttactgta
    46381 cgctccgctg ccaaacacat tcaggcagag cgtccatgcc agcagtgcca gagatttgtc
    46441 cggttgctgc atgagtgccg cctggactgc cagcgtgcgt tcggaagaca ttttcgtcag
    46501 cagtggcaga ctgatttcct ccacgggcgc tttctcctgc acctgttccg tgcggtcagc
    46561 gtcgtcagcc gcgtcatctt cactgcgcaa ctgcacacca cgctggacac atacgttgcc
    46621 ataacgccag gacaccacca caccgcttcc ggcacgtatc tccggcgtcc acgctctgac
    46681 cttcgccatg cagcgcatca gcttcatttc tgcttccagc aggtcggatt cctcacactg
    46741 gttttccagc gcgtcgtaac gcgtcatcag ttcgttcagg cgttcgtctt ccgcctccgt
    46801 cagcaccgct tccggctccg gcagacagcg gtatgttccg gcatcctcac ggcactcacc
    46861 gacaggctcc atgcgcccgg cgcaccattc ccagccttcg gcttcccgaa ggtgttcagc
    46921 gacagcctgg agtttttcca gcagggcggc atcgagcgcc acgcggtcga cataaccgtc
    46981 cccgtcatcg ctgaacaaat cggtgcgcag ttcgtctggc gagaaggcat cagcccccac
    47041 gaagcggaat ttactgttcc cgtccaccgc cacttcactt tcggtaatca ggttgcggat
    47101 aacccgcaca tccggtttac cgccccatcc tgactggcag gcggcttcaa acacctgcac
    47161 ctgacgcgcg gtgtcgttct ccagcgccag cgcctgacag tgttcggtgg tgatgcggtc
    47221 ttctgccagc gcatcaagga tgacgggtgc aaggtctgcc agtttcagca ttcgctggac
    47281 gtggcggggt gaatagccca gcaggtcgcc agtctgcgca ggtgttttgc cttcctgcgc
    47341 cattgcgcgg aatccggcaa tctgttcggc tgggtgcata tcccgacgat gaccgttctc
    47401 ggtcatcgat gcagccgtcg ccagttcctg cggaataatt ttcacgcgga caggccagtc
    47461 agccggaatg atgccacgct ctgccagcat attgagtgct gccagtctgc gaccacctgc
    47521 ggcgacaccg tggcggtcac caggcagggc atgcacgacc agattctgca gcaggccaac
    47581 gcccttaatg gactcagcca gttcgctgac ggactccgca gaatacggca ccgtgcgcac
    47641 attcagtggt gacttaatca gcgaggccag cggcacgctc acttcctccg tctgcgccag
    47701 cagggcagac aggactgttt cctgcgtttt tgcagattta cggcttgctt tgcgggtggt
    47761 gtctggttca gactttacta ctgacatgat tattcccctt acggtttagt taatgtctgc
    47821 cggacgtcat ccccgtccgg cacctctctt acttacgaat gccgaaatcc ctgatgtacc
    47881 ggttcatcca gtacccggca cagtcctcaa aattccagga gcgccagatc aacgagccat
    47941 cctgacggac aaccaggcga aaatgcgttc cctgatcatc ctcgataaag acattccggt
    48001 actgtgcaac aacggtttca gcacactcgc gggtgaatgc cccatcgggt agttcctgaa
    48061 tcatttcatc ggatttgttc atcatcgttc tccggtaatg ccccacacat ggtggggctg
    48121 tcagaaaaat cagccagatc agaatggcgc atcatcgtcg aagccataag gaggttctgg
    48181 ctgttgttga ggctgaggct ctggctgcgc ggccttacgt ccacggcctt tcgttttcgc
    48241 gccacctttt ttcgtcgcgt cagcactctg tggctgccca ttctgatgag gcttcggttg
    48301 cgcctgagcg ttctgctgtg gtgcacgtcc cagcagctgc acggtgcccg tggtcttaac
    48361 aagaatttca gtgacgtaac gggtgatacc gttatcttcc cagctacggg tgcgaagctg
    48421 accttcgatg tagacctgcg cgcccttgcg cagatattca cctgccactt ccgcgagctt
    48481 gccgaacagc accacgcggt gccattccgt ctgctcccgc atctcccccg tctgtttgtc
    48541 acgccagctt tctgatgtgg ccacctgcag gtttgccact gcgcccccgt tggggatgta
    48601 acggacttcc ggatcattgc ccagacgccc gatgaggatg accttgttga taccacgtgc
    48661 actcataaga acctcctgac tgaaagatgt gagataaacc ggataccggc gacgggacgt
    48721 tcatgtgccc cttcagagga gcgaaagctc ctcctgccgt ccacttcaca ggggtttctc
    48781 cccgcagtgg acctttccga tgggggcccg tttaccgcct cacgggacag ctgtccgggg
    48841 gcggacgcaa aatttttagc cgggttcatg gctaaaaatt ttgtggccgg tgcatctcac
    48901 cctggacaga tggaacggga gggggttacg tctgcccccc ggaaagtcca cagcgggaaa
    48961 caggccgcag gggtggcggc aggagggaag actgctgcgc catcaggcgc agtttcctca
    49021 cacaccgtca ggtgtgcagg gagcgcaggt cacggcgcga caggggcggc gcgaccggaa
    49081 cggtcgctgc aagccgggtt ttcacgcatc ggaccgtagc ggcagcccga aaccgcgcca
    49141 gcgggttcgg cggagactgg cgggtgcgcc agcagctgcc tgtcggagtc gggcctgacg
    49201 accggatgcc gccaggcagc cgggagccga agtgggcgca ataaaagaca gccagaaacg
    49261 gtgacagcgt cacggaatat cacacctgtt ccctgcatac agccatacac taccttctga
    49321 ccggaaagcc gggccggcag cgccgcaggc gctgcacccc gcatttccgg caggcgtcag
    49381 cctgccaggg gttttcgcgc gggctgacag gcggcctcca ccgcctgccc tgcatcacct
    49441 gtttctgctg tcctgtaaga tgtagtgacc gcccaggaaa acagcctgct gattcgccac
    49501 aaaatcgcag atttcatcaa caatctgcgc ggtggtgagc ggtttctgcc ccatttcacg
    49561 acgccgcgca ttaatcacgg ctttgtaatt acgcagaatt tcgcgggttt ctgtgctgat
    49621 attcatgaca tcgtctcccc gtccagcgcc tgtaacacaa cgtccattgc ttcgcgttcc
    49681 tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata caggcgcttc
    49741 atttcactgc ctgccagcgc catcactttt tccagcatca ccgccgggac tgtcacctga
    49801 cggctggcgc gcttttctac catcagctca cgaacaacgt cgtgaacaac gctgacatcc
    49861 gcaccatgct caaaggcatc gtccagccgc tgcatgacgg tttccagctc atcgtctgac
    49921 aggttccagt cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaacg
    49981 cattcactgt ccggcacgtc tgtatccagc acttcgcgga taatttcttt aacagtacag
    50041 tacattttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag
    50101 agccgcgtaa atcgcgttgc attcagggtg aaattcgatg tgacgccaca actgcgcatc
    50161 gcgcatccgg taaagctggg tcagtcctgc atcaccgctg tcgtggtaca gccacaactg
    50221 gcggttaatc acaagggaag tcagaatgat gccgcaggca tcagcactca cggtacggtc
    50281 aaaccagttt gcaccgttca ccatgtggaa acggtcgcca tccggcatca tgtaaccgcc
    50341 accgtccggc aggcggatga agtgccagaa gccgccttca tattccggca tcatttgttt
    50401 cgccagcgcg tacacagacg cttcggcata cagaaaatcc aggccaaaca ggtaaggcag
    50461 aaaagacaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat actgcatggg
    50521 atttcctccg ttgttcacat attccgcgac accgggcggt gccgtgcctc cataaaagcc
    50581 gggcgataag gcggggatgc aggggcgcaa gggaagccgc agcctgacgc acacggctgg
    50641 cgggagttgc agggtgaggc cgcgaaacct gagcgggcga acgaccggaa aatcacggca
    50701 gacgggaagg agggaccggg tgaccgcagc ggcgaagcgt acccttgcat caacgccagc
    50761 ccggctaaga tggaggcacg gcacagcccg cacacggcgc tgaggccaca taactgaaac
    50821 cccataaggc catgcccgca tggccgggta aagcggagcc ggcggcgact tcctttgtgc
    50881 tctgtccggg accggcagga aaaaggcggg gattatcccc gccatcatca gcccctgatt
    50941 tttgccaggg catccgccat gctgcggatt tcggcccggg cttcccggac cacctcccgc
    51001 agaacaccac ggtcaaacca ccggcggggc ggttcatcag gtatacgcag cacatcgcag
    51061 gccagcccac tggacaggat atgctccaca cactcactga ccagatacat ttccaccgcc
    51121 gaaaaacgtg ctccccggtt caggatggca tcgagcacca cgggcaggtt ttcgtcgtct
    51181 tcatcgagcc agcaggggtg atcgctttcc ttcacgacca ggcgaaagcg acgcgtctgg
    51241 cgggtcagca acgtctgacc ggtggtggtc agcgccgcac ggatacgccg cgcatcttct
    51301 gccacctggt aacagatgct ttgtaccgca tcgtgcaccg catcaagaac agccctgatt
    51361 tcgtcttcgg tcagccattc tccggcatac gggaaagaca cctgcgttcc gtctgccgga
    51421 agtaccggca gcgattgcca ctgactgccg gacagtacca gttccgcccc ggttccggtc
    51481 agtctctgaa tggcggaggc caggagcggc ggcagtacgc cgccgtgacg gttgataagt
    51541 aacgtcgcca gccggggcgc atccagccgc acgtaaatgc tgatccagcc cggatagttc
    51601 ggatttcgct ggtcaacatc cgtctcttcc ggccagccct gcgccagcag caggttacgg
    51661 gcgtgacgac aaatcagtgc attcagcgtt gtgttttcgt tcatgattaa tcgttctccc
    51721 tgaatcgccg cccggaggcg gcgataacat cagtcgtttt cgtgtgtgtt gtttttcagc
    51781 aggctgcggg cctgattaac caccgcctga cggcgggtga cggattccgg gacggcgagg
    51841 ccgttatcac gggcggccca ttcacgtttc cacagtgcct gttcagcggt ctgaatcagg
    51901 gacgccagca gttcagcaga gacggtcacc aggcggtttt cgctcacgtt gctgataatc
    51961 tccatcgcag cggcggaaga aataccggac tcaatccgct ggtcttccgg gatgtcctcc
    52021 aggtgcgcca ggacagtcct gatttcatga tcggtcagag aaatttccat accgtcggcc
    52081 agtgcctgaa tctcttccgg ggaccagata agcagcatca gcggcgtgtt tcccggatac
    52141 tttgctgcca gctcacggca gagcgtttcg catgttccgt acatttcgtc tcctccttaa
    52201 tctttgaagc acgcatccag ttcaagaatt tcggtatatg cctccatgaa caggcgattg
    52261 gcgcgataca gcgtctggcg tgccaggcgt tcaacaagca catcggaatc aaagcgacag
    52321 cgatgctcca tatcgttcag cctggcccga aggtcgcaca tggcattggc cacctccgcc
    52381 acttcggcgc gaagtttttc gtgtccggca tagttcattt acgcagcctc cggcataaac
    52441 cagtcatcat tcgcggcccc ctgctgcatg gcccgttgta cggcagccag acgttgctgt
    52501 ccggcacggt gatactgctc aagcagctcg ataccgatat accggcgtcc ggactggagg
    52561 gcggcgacgc aggttgagcc gctgcctgca aacgggtcca gcacaatcgc gttcgggtgt
    52621 gtgaagctct caatcagcgg ttgcaggctg gtaacgggct tttccgtcgg gtgatgacgg
    52681 ttgcccgaat atttccagcc cagcacgtcc ggcagcgggt tttgtggcag acgtggacgg
    52741 ccttttgcca ggatgtaggc gcattcgtgg cgatagccca catatgcggc cttcgatgtg
    52801 tagtttttgg taaacaccag gtgtccgacc acgctgaatc ccgcattttt ccaggcggcc
    52861 acaaagcgat cgacgcggtt ccagccatag aagctgacca tcagcgcatc ttttttgagt
    52921 acgcggtaca tttcattaca ggccggttgc agccattcat cggttttatc gccagcgatg
    52981 gtgcgcccgg aacggtcacg gaaaccgacg aggtacggcg ggtcggtgag gatgaaatca
    53041 atggcgttat caggaatacg ggccataaca tcgatgcagt taccgaggac aaaacgggac
    53101 atacgaaact ccttctctta cagagggcac cgggagggca cccctgtggt gcccgttccc
    53161 cgctgcctgt gggctgttca ccgcgtccgg taacgggtca ggccgaccgt ggaatatcgc
    53221 agccgtcgtc tttttgacgg cgagaatatg ccgcgaaagc ggcttgaagg gcgttcatca
    53281 gaaaagggcg acgggctggc gtacactgtc accgatgcca gccgggagcg gtaaaagcgc
    53341 ccgtcccgtg cccggccgga cgcgggacac tgaccacagg cagcgtttat gccgcgccgc
    53401 gtcagccggc gcagtaaaaa gaggagcacg gcgacatcct ttgtcggaaa agaaacgggc
    53461 ggtttcccgc ccgtctccgt catacttcat ccgccataat gcgccacatt gtcgactatc
    53521 cacgcaggtc cggtgacttc cggcagctca tcgtcatctg tcggggaaga ccattccagt
    53581 tccccccaca gcacatcaac gagctctccg cgctcataca actgccagcc acagaaatca
    53641 cagccctgtt cggcatacca gtgctccagc gtgcagctga aacggcggct taattctgca
    53701 ataacgtcgc tttccggctg acaccacggc gtgtcgaaat caacctgaat gaagttgtct
    53761 ccctgactac tgatattcac ctcatacccc acaggccact tcacgccata ccgttccgta
    53821 taccagtgat atgcagaagg aacaccgttc agaacaccac cgttaaagcc attcacttca
    53881 acatccaggc gagtcggcag aaccgtcagc aggtcaaacg gcatggtttt ttccggcagt
    53941 acgttgtcac acagacggtt ccaccacacc gatacatcct cgttactcca gaagccacac
    54001 cagtcacctc tctttgcggt gaaaagtgcg gtgatggtat ccctgacatc gtccggtaat
    54061 ccttcccagc gacgctgttc agtaccgctc tgcagccaga gttcatgcag catcaggcag
    54121 ttttgttcat ccagtagcac accgttctgc aggtgtgtca gccagcgtgt gaacgcgata
    54181 ttctccggcg atacaacacc acgtccggca gccgtcagtc cggggcacgg ttcaaactgc
    54241 acatcttcag tggtctgcag aagtccggca cttccggcca gaaacagctg aataccttca
    54301 tttgtggcgc ggcgataaaa cggtgtgact gcgccgctgg ccagtcgttt aatctcagcg
    54361 atctgtgccg gttcaccaga aaaatacata cgattcgagc accagtttgg catgggaatt
    54421 ctcctctcac atgcggcctc catcaggccg tgccgcacct gtaaccgggc gaccgcatac
    54481 gaatgcaggt gcggccccga agccgcagcg cgtggaggag gaacgacgaa acgtgaccgg
    54541 gtgaggaggc cggcgacagc gacacacttg catcggatgc agcccggtta acgtgccggc
    54601 acggcctggg taaccaggta ttttattcac agaaccgtgc gcaaaatgct gtggataagc
    54661 tggacacagc agtccacagc aggcataccg ccacaccctc agagaattga gcgtttttta
    54721 gtcacaataa tgacatttga ctgtgtgtgg attcagttat tagaatactg tatatgcata
    54781 cagcaaaaga aaagggagat gagaacatga tccgcattga aattcttttc gaccgccaga
    54841 gcacaaaaaa cctcaaatca ggcacacttc aggcgctaca gaatgaaatc gaacaacgtc
    54901 tgaaaccaca ctacccggaa atctggctgc gtatagacca gggcagcgcc ccgtctgtat
    54961 ctgtcacagg agcccgcaac gacaaggata aagaacgtat cctgtctctg ctggaagaaa
    55021 tctggcagga cgacagctgg ctgcctgcag catgagtacc gtttatcacc gtccggctga
    55081 cccttcaggc gatgacagtt acgtgcgccc gttgtttgcc gatcgttgcc aggccggttt
    55141 tccttcgcct gccactgatt atgctgagca ggaactggat ctgaacagct attgcatcag
    55201 cagacctgca gccaccttct ttctgcgcgc cagcggtgaa tcgatgaacc aggctggcgt
    55261 gcagaatggt gatctgctgg tagtggacag ggccgaaaaa ccacaacacg gggacatcgt
    55321 tatcgctgag atcgacggtg agttcaccgt caaacgactg ctgttgcgcc cacgcccggc
    55381 actggagccg gtttcagaca gcccggagtt ccgcacactg tatccggaaa acatctgtat
    55441 ttttggtgtt gtcactcacg tgatacacag gacgcgggag ttacgctgat gtttgcactg
    55501 gctgatatca acagtttcta cgcctcatgt gaaaaagttt tccgcccgga ccttcgcaac
    55561 gaaccggtca tcgtactcag caataacgat ggctgtgtga tcgcgcgcag cccggaggca
    55621 aaagcccttg gcatcagaat ggggcagccc tggtttcagg tgagacaaat gcgcctggag
    55681 aagaaaatac atgtattttc cagcaattat gcgctgtacc acagcatgag ccaacgggtt
    55741 atggctgttc tggagtcgct ttctcccgca gttgagccct actcaattga tgaaatgttc
    55801 attgatttgc ggggggtgaa ccgttgcatc tctccggagg tttttggtca tcagctcagg
    55861 gaacaggtaa agagctggac aggactcacc atgggggtgg gcattgcgcc tacaaaaacg
    55921 ctggctaaaa gtgcacagtg ggcaacaaag caatggccac agttttccgg tgtggtcgcg
    55981 ctgacggcag aaaaccgtaa tcggatcttg aagctactgg ggctgcagcc agttggtgag
    56041 gtctggggag taggacgcag actaacggaa aagctgaatg cactgggtat taacacagca
    56101 ctgcagctgg cgcaggctaa cacggcattc atccggaaaa acttcagcgt cattcttgag
    56161 cgtacggtac gcgaactcaa cggcgagtcc tgcatatccc tggaagaagc accaccggca
    56221 aaacagcaga ttgtctgtag tcgcagtttt ggtgaacgaa tcacagacaa agatgccatg
    56281 caccaggctg ttgttcagta tgcagagcgg gccgcagaga aactacgtgg ggagcttcag
    56341 tattgccggc aggtgacgac atttgtacgg acatccccct ttgcagtaaa agaaccctgt
    56401 tacagcaatg ccgctgtgga aaagcttcca ttgcccacac aggacagccg ggacattatt
    56461 gccgccgcat gcagagcctt aaaccatgtc tggcgtgaag ggtaccgcta tatgaaggca
    56521 ggagtcatgc tggctgattt cacaccatcg ggtatagcgc agccgggatt atttgatgaa
    56581 atccagcccc gtaaaaacag tgaaaagtta atgaaaacac tcgatgaact gaaccagtcg
    56641 ggaaaaggga aagtgtggtt tgcggggcga ggaaccgccc ctgaatggca aatgaaacgg
    56701 gaaatgttga gtcagtgtta tacaactaaa tggcgagata ttcccctggc caggctgggt
    56761 tagttcagtc atctccgaac atattttcag cgtttcttct ggtctggtct tcaccgtcat
    56821 ttctcgacag actctgctct gtaagaggag tactgcaatc cacagacgta tggagaccag
    56881 attgtttttc cagtaattca agcaatttct tttcaaccga aaaagcacct ggtatcacgc
    56941 tggaaatcac cttattaata tcatccagag tcgtattttc tgtcagtaac tccgatatca
    57001 gcaaaggaag acgtttatct atagctgcaa acgccgcacc agtaactaac atcgcgcgca
    57061 tgaaacgtcc ccgctccttc agtttttcat ttgcaatgag ctcatctgct atcttatctc
    57121 ctgtccgttc atccgggaaa agttcaaatg agcagcgtct ggatttattt tcatcactca
    57181 ttagtcacac cacctttcat ttttatcatt cccatgacta aatcaaactg cggggatgag
    57241 ctcagataaa agcgctcatc aggaacctta gtcaggttct tcacagcctc ggccacaatt
    57301 tcagccccac caccaacaca cattacatgt gtatagtcgg tgaaacggct gatgacatca
    57361 ttaactcgtt taataagcaa cttctgccgt tcattgataa cattaatgat agactcacgc
    57421 tgttcagcat tataaatgcg acgggaaagg tagtcaggtt cattacggtg cacaatgata
    57481 ttgtcagcct ggaatgagct cactcttgta ttagcatgta cagccatctg ttccttaacc
    57541 ccggatgtaa tcagggacac gccgatattt ggatcacacc aggtttttgt aatccctgtc
    57601 attttgctac gaacatgcga cacatcaaga gttgtaccac cgagatctac gatcaaaagg
    57661 gattcatcat cctcaagccc cgcaagaaca ctgaaaccag cgggaatact ttcaggaaga
    57721 acggaaacac tgcgaataac aaatgcatca ctcccctgca cccgtacttc acgcatcaca
    57781 ttttttttct ttctttcaat gttttgctta tttttctgat tgttggcgtc aaggtattca
    57841 gagatcggca atgtgacgat gacatcaaca ggctgcgctt tcaggccggt ctgctgtagc
    57901 gcatgctgaa tggccacaac attcacatca ctgtactgat agcgtgtttc agtcgttacg
    57961 acagcatcag cacttaatgg atcaaatgaa aacttctcgc cgtcgatctc gtaattagcc
    58021 ggggcagcat catccagtaa actaaaagac cattccggtt taaagctgtt agggctgatt
    58081 aacgttttca cgtcaccatc ctccaaccag gcaagtttaa tgttggtaga accatcatca
    58141 ataaagattt tcataaccag tctccgttgc tcataaacaa atagaaaaaa caaacatgct
    58201 tcatacatgc aatgtatatt caattataat gaaaaaacgg tctttctgaa aggacaaatg
    58261 caacataaaa acacgataaa ttgcatgtac attgtacaca caactttaag accagaggaa
    58321 atcatagata caaaaacatt atgaaggtta tatgtgatac ataaatgtaa tataaaaaca
    58381 tcgtggacat catggttcag gttaggggaa aaggaatgaa atggtgaaac tgagaggaaa
    58441 ggaaagcggt gacgaatcat gtcaccataa acagaaagat gtctaatggg gactctttag
    58501 gtacgtgaag aatccccata acgcatttaa aagacacaca tcagataccc ttaaggtatc
    58561 cgatagttta ttgacctatt gcaccatcac gttcaagctt ctctcttata gcctcaataa
    58621 tatatgagct aaaatccaga gatgttttac tggtcgcttt aagttccgca tgagcttcaa
    58681 aatacgacag cggtatggcc ttaagattct tagccttcgt cctggcagcc acgggacgac
    58741 ctgaaacttc ctctgcacct tcatttggct gagacggggc tgttgctgtt acggcagaag
    58801 aagaagtttg taatctggca gaatccacca ggtttttcag tttttcagcc tgtgcactaa
    58861 atttcgcttt gctcatgaga taacccagga tgtttgttag atacttaaaa gatatccatt
    58921 aaaagcccaa aggatactat atggataccc aatagacaca ccttactgtt tgttattttc
    58981 aatcagatga ttgatttctt cgatcagggc aataacttcc ttacctcctg ccgaatagcg
    59041 cccatgaaca gactcagtga ttcccatccc agtctcaatg acatcctcaa attctgcacg
    59101 agcagaaatg cggctcttca tcagcttcag atgtttgaac gatggcagga ttgcatcaag
    59161 cttagggaat ttcttcttgt taggattaac tttacagaga tacaggtgag cagttatatc
    59221 ggtccccatg atagagctga tttcgtccag tactccatca aaatgcatca aaccaatacg
    59281 ttcagtcagg gagtcttttg aagggacaat aacgcagtct gcaaaggcaa tcgcagtacg
    59341 ggtaagatct gagtcaaatc ccccacagtc aataagtaac gtcttatcgc tgttcttgta
    59401 gggagtcata atttcgatta actcatccac tgttttgggt actttcacgg aaatttcttt
    59461 tccttcaggt ctcagcccca gaatgataga aagcccggta tgagtatcca gatcaactgt
    59521 atcagacttc atttcaccaa caatgtttac ggatgaagtt gtcttaccca caccaccttt
    59581 gggatgcgta ttagcaataa caggcatagt gtatctccag aatccacatg taatacccat
    59641 aaagccccca taagacacct ttcggatatc caagaggtac tcaatagatg tttttttact
    59701 ctataccggt cctgcatgtc aacagaatat gaaataccga taatatatcc atgagatatc
    59761 cgaaagggat ttaaaaaagt cagagcttat ggaattcttt tgagcataga caccgcatcg
    59821 gactcaggca tcgaaaactg cacccggtgc cgtgcagcca catccagtgc aaacaccttc
    59881 gtgtagacct ctgttgagct gatggactta tgccccatca gactctgcag aaccttaagc
    59941 ggtataccgg catacagcat gtgcatcgca taggaatggc ggaacgtatg tggcgtgacc
    60001 gggacagaga acgtcacacc atcagcggca gcggcggtaa ccgcctcccc aatccaggtc
    60061 ctgaccgttc tgtccgtcac ttcccagatc cgcgctttct ctgtcctgcc tgttcgttta
    60121 ttacgccgtt ccatggggat tttcagtgtg gccaccatcg tctgcagctg gctgacgtac
    60181 caggcgtcgg agagcggaac cagacggtga gtctgctgac cgacgggcat ccttcctgcc
    60241 gtcctggcgg ctttttctgt ccgctgcttc agagtggcca gctgcacaaa cggatacgga
    60301 ggcgtgagcg aaaaatcccc ccgcgtcagc gccagtgctt cattaatgcg cgcaccggtg
    60361 ttccacagtg tggccagcag catcttgcgg cgcagatccg ggacgtaatg gagcagggcg
    60421 ctcacttcag gggccagcag gtattttggc agttcatcat gaaccatcga catctggcgg
    60481 agtgccagcg ctgccggata atcgatggca acaggaagct gagcagcatc ccgggcatgg
    60541 tggatcggtg ccggcaggta ctgcatatca gtgttcactc cacggggaaa cgtcgggcag
    60601 gttttccaca acacggtact ttcccggata acggagctgg ttaccgccac gcgtccagac
    60661 ggtgacaaca aaacccccgc cctggttgtt ttcggggagc aggcgatcgc actgctcact
    60721 cagcatgtgg atcacaaacg gcgcatcgct gccgtcttca aataccagtt ccagcgcatc
    60781 gcggccatgc agcgttccct tactgatgat gacgtactca gccccccgca tctccctgag
    60841 taaatgtttt gccgcatccg ggacaagcag cctggctgcg ccggcattcc agctgaggta
    60901 gacatatccg gcctgcgcct gtacagactg ccagtaatcc gttccggcga cagcctgacc
    60961 ttcattctca ataaaaaaca tgggtgactc ctttttcttt tccgggcagg gacggtcatg
    61021 cgataacgtc atccatcatc actgccctta ccgtcacgcc gtctgataac atcatgccgt
    61081 tatcatcatc accatgataa caagcatcca tataaccacg ccgttaaatt accggagagc
    61141 cgtttccgtc agatccgact ttccggaata tcacaacttt ccggaagtgt accacgctga
    61201 gaaaataatc atatcgctgt tttccgcccc tgcgcggcgc atcacaggcc agggagatga
    61261 cacaacatat agtgtcatta gcagctgaat tcagcactac atgttgtgct gacaggaggt
    61321 ggcgttgtgt ggggggagag gaagatccgt ctccgccgga aaccggatct gcacgacggg
    61381 aaacgggcaa atgatatgat aatttagcaa ttaacataaa atcatatcag atatgatttt
    61441 catcattgat gataaaacca tatctgatat tatcagaaat caggagcaca caaatcatgc
    61501 atgatattat ttttacctgt cagaacatta tcagatggag cgtgcaggat ggatgaagaa
    61561 agggtgtttt cactcagcta tgaacaactg acccggtttg cggaaaaacg catccgggaa
    61621 tgcaatctgg acagccaggg cgcgatttat ctctgcgagt cagccaaagc cggagccgtg
    61681 ctgatcttct ggcatgaact ggccataaat ggctatgcct caatgaatgc cataaagaga
    61741 caggagctca ttgacgccga ctttcagcgc ctcagaaacc tcatctggcc ggaggatgac
    61801 aggtaatgac ccagagccgc cgaccgtcgc cactgcagcg gagagtgctg attgtgctgg
    61861 ctgcccttga tgaaaaacgg ccgggacccg tgctgacgcg ggacctggaa cgggtgctgg
    61921 aacgaagcgg ggaggccccg gtctacgggc caaacctgcg cgcctcctgc cgtcgtctgg
    61981 aggatgccgg ctggcttcgt accctgcgcg ccccgaatct gcagctggct gtcgagctga
    62041 cggacgccgg ccgggccgtg gcacagcctc tgctgctggc agaacaggac cgcctccggg
    62101 cagaacagcg tgcggcagaa gtcgtcgtac tgccgctggt cccggccgca ggtctgcccg
    62161 cagacggcac ttctgccact gatctggcgg tggagctgaa cggcatgacg tatcaggcct
    62221 gccgtgggga ttttgtggta cgtctcgacg gcagcacctg tctgcagtta tggaataaag
    62281 aaggcagggt ggtacgcctg gagggcgatc cgctggaagt ggcgcagtgg ctgcaggcct
    62341 gtcacgatgc cggaatggag gttcgtgtac aggttaatga aagcgtcacc ccgtaaaaac
    62401 ataccagcac ggtttacacg tcatcccatg atggcagccg gttaactttc ctgcgccacc
    62461 agtggaaaga ggccatgagc agtccgaaca aaaaaccacc cgtgatactt ataataatgg
    62521 cctcacctgc caccattcct gacggccccc agtagataaa ccacattgca catccccagg
    62581 aaatgcccca taagctcccc atcaacagcg tgacctgcca gaacggcata aagggtaatg
    62641 gcggaagccg gatacccagc cgccagagaa tacgcagaag aggaggggca taattgctcc
    62701 gccacatatt tttgctgtcc atcagggcaa tggcccgggc ttttttctgc tcaaaggtca
    62761 caggagcacc tccgttccat gatgtaaacg tgacacagct tattgttatt gattattatt
    62821 atcatactgt gattatgtta tcacgcaccg gtacacgata acaataaagg agaaaccaga
    62881 tgaaactgga tatatctgta aagtatttac tgaagagcct gataccaagc ctcattattc
    62941 ttacagtttt ttatctggga tggaaagata accaggaaaa tgcaagaatg ttttatgcgt
    63001 tcatcggatg cattatcagc gccattactt ttcctttttc aatgaggata atacagaaaa
    63061 tggtaataag gtttacaggg aaagaattct ggcaaaaaga cttctttaca aatccagttg
    63121 gcggaagctt aactgcaata tttgaattat tctgtttcgt tatatcagtt cctgtggttg
    63181 ccatttactt aatatttata ttctgcaaag ccctttcagg aaaatgaaag ggcatcatta
    63241 tgtgtcagat accaataagt ttattgacct gctcaataaa cttatcatta acaagcgcac
    63301 tcactgcagc cataataatt gcaaaaccca gaatacctac aggggttccc agcatgacgg
    63361 aaaacgtcca tgctgtaact gctgaagcag ctcttcctgc tgccagtgat tcaagtttta
    63421 caaaaaatgg acgccagttg tctgttttta cagccttttg taactccaca gcaacatcat
    63481 aacgatcaat aacttttccg gtaaaaccaa atgccttact gaacagctta aaattctccg
    63541 ccatatggac ctgattaata gcttccaggg ctttagaaat agccatgcga tcttgtatgc
    63601 tatatttctt gttcagatta ttacgaaatt tatcaaaagc gttcagtgca tcatccacac
    63661 tcttaatctg tttcccttga gatacagaag ccagctcctt agcaagttcg gatgcttgtt
    63721 taccgaactc attatatatc gttctgtaga aatcagaggt gagttttatg gcatccttta
    63781 ccatatttat ttcatccctt tttcttttct cttcagctat tttatgattg atgtcagcaa
    63841 tctggctacg gatattctct ttttccttca acagggcatt aagagcgtca tttgcatgct
    63901 tttgttcatt tgttctggca ctgacgttat ttctcgccga atttaccgca gattcagcag
    63961 aggtgatttt atttctggca tcaagcaatc gttgccgtaa cttatcccat tcagcaactt
    64021 cagcattata aattttcttg tcaccttcca catcctctgt caccaccgga ttccggtcat
    64081 tcagaatatt gcgtttatag tccagaccgg aatgggtcag aagataattc aggtttgcac
    64141 ggttatcgat gaccgcgctg gttgtgattt ttatcgtacc ggcaaatctc ggatcaccac
    64201 tcacaacaat agaatgattt gttgatgaac gccccgggaa tttttcaggg gaaactatcg
    64261 ttttgccgtc aggcgtgttt ttctgttgcg tcagctgttg ttcagctctg gttaatgcat
    64321 tacgggccgt gttaagcgat tccgtcaccg aactgagttt attccgggcc tctgaaatgg
    64381 ccctttcatt ccgtgtctgc tgcccgagca gacgggcctg ttgtgcatca agaacggcaa
    64441 gggctgcccc gccatttttt tccagctctg acaaccgcgt gtccagaata ccttttctgc
    64501 gctcgtattc agcaagcata tcagcggcct tcttgtctgc gttctgcgca tccttctgcc
    64561 gtttttcagc ctgttcgatt aacgacctgg cctcccgggg agatatacga agagaatcag
    64621 catcagcaaa gagcatccgg ctctctgtat gcagccggag ggcatcatat ccggcgattt
    64681 cagtacggaa tccgtattct ctcagctctg cctgcaggag tctgaactcc tgtcgtgtaa
    64741 tatcagcggc atctgcgttt ccggcacgga gtgttttcag tgtgttttca tctttttccc
    64801 ttgcagcaat cgccgcagag aggcgttttc cggcttcagt acgcttattc tcattctctt
    64861 tctgctgcgc gctgagcgtg tttttgtacg ctgtgatttc cttgtccctt ttttcgatat
    64921 cacttcgggt tggggaatca tcgacccact cgtttccgcc ccagatggtt ttgttcccga
    64981 aggaactcca tgcaggcggg gtaccatgaa agacatcgac acgtggaggg tttacataaa
    65041 tatcaacggc cataatttca tggccatctg aatcataccc cagcgattct gcaccgggat
    65101 ttgtaatacg tacagggtca gacattcggt atctccttca tcctctgagg aaaacaaatg
    65161 aattaactgc ttacgtatat ggatacatat acagtattaa tttattaagg agaaaatggc
    65221 atgtcaagat ttaatgatag tgattatcat taacatgaag tgatgttaca ccgttcagaa
    65281 agcctgctga cgctgtggca gcatcacagt ctgggtattc tctggtgcaa tacaaaaaac
    65341 ggactttccc gtaacaacag acaatgttac gggaaaagtt acagaactga gccacaaaat
    65401 atctcagtac ctgagatgca taaaaacacc gtacatatgt ttctcaaaag ggggatgatc
    65461 gttatccagc catttcggtg gatcggtatg cagaacacca atctgcttca tgccggtatg
    65521 ctgcctggat tttctcgcgc agtaatcact gtcttcatat cccatcgttt cgatgaccac
    65581 tctggccgtc tttccgtcag gagcccttgc tgtgacaatg aaatccggca ggacatattt
    65641 cttttcacca ttaacataaa cttctgtatc aaacaggggc ctttccagac ttaattccgg
    65701 cgtacctggc tttcttttaa gccagctggc cacattcaga agagcagtga gcgtgtttcg
    65761 ttccagtttg ctgtccacag gcaccggaca tgtcagttga taaagagcat gagcataccc
    65821 ttcactgcag atgattctgc catcggcact ccggcaaaaa gcaagaataa cccagtaagg
    65881 gggacgtatt ccgccctgcg cttcaccatt aatactgatc ccccgcacag gtctgatgac
    65941 ctgaatatta ccgttcgccc agtgaatttc cacagcatca cgacagatgt gctgcgccat
    66001 aaaaatctga aagaacatat gctttcttct ggcaggccag tgtcgttctg aattctccag
    66061 cgtttccatt aaccgctcct gagacctttc agacattcca ggccggaaat ggataatttc
    66121 agataaggat cttccccgaa tatactcctg ctgcagtgta acctgccgta tggcatcaag
    66181 acactcacga taattagtcc ggatacgggg attagccagg gaatccagtt tattcagccc
    66241 agcatcgtca ataagagaca ggagtaaacg ggcaaggcgc ggacgtttct tgcgaacgga
    66301 ggagctctca ccattatggt aagcagagcg ggcctgcagt ctgataacag aattactgtc
    66361 atcaggtggt aaaaagtttc tgtaatttat acgggtgcta acgggatgcc ggcttgctcc
    66421 ggaagatgtt tcctcttcat ttccggataa ctgacgatac atggggcatt caggtgcatg
    66481 ctcatgatta aaactggaca gataaagagt acccgtattg tttttaagag cagcaaaatt
    66541 catggccggc gaatctcccg gaatacagtc acactgtaac catgcatcat actcacgcat
    66601 ttcccggaga atatcattgg ccaggttatg tagtaccatg gttgctggct ttttcagtcc
    66661 acagaccgtc tttactgcag tctcttcctc actgctaagc tcccgaatca ctttgccccg
    66721 gggattcttc tttacaagta tcattggcat aatgaaagca ctccttatgt acatgcttaa
    66781 acagtaagta aaatatacaa agaacacccc atgaaacaaa ggcagagatg aactgtgcgc
    66841 cccggtatct tcttctgata tcctttcgaa tgaattgttt ttatttatat catgacaaca
    66901 ttaaagtgag agaatatatc aaccatgttt ctgtcagggt tcagtagaat atcaattcga
    66961 ctgtcacgcc ataactgtta ctacctcgag tataaatatt cagagaacgg acttattagc
    67021 catgaaaaat cacctgatgg aaaatttact aatttatgga ttgtgatatt attaaagagt
    67081 aaaaccagcc agccgaggta tttatgggaa aaaatcagga agaaaggaaa actcccgtca
    67141 ttgtggtgaa aaaacgtcgt actttttcgc caccgtcctt atccgaaaaa acagatatta
    67201 tcacacctgt atttactgaa cagacggcag aatcagcccc cgccggaata aattcatctg
    67261 ccgtcgaaac acatatcccc gaagcgccag ccaggaagaa gaaaaagaaa agacaccgtt
    67321 ttccacgtcc ctcccactgg acccgtgagt atactcacga gtgcgtggaa aaaataaagg
    67381 ccctgtttcc tcatcttcgt gctgaaggtg gtggatttat tcccctgaaa attggcataa
    67441 acaatgatat ttcggcattt ctggcagaac acccggagac agaactgacc atggacgaat
    67501 ggctctgtgc agtgtcctgc atcacatccc gccgcgtata tctgcagcgg acagctgtcg
    67561 ccggcgtccc acgttatggc ctggatggtc accccaaagg gcaggtctca gactctgaag
    67621 cacagagtgc cggtcgcaga ctggcgacat tagaacaaaa gtggctcagg atgcaggcac
    67681 agcaggagaa tatttctggg caataaacac tcctgataac aggtggacat tacgtatcag
    67741 aaaaaatggg aagagaatgt tatgtgtgat tcagaagcgg agaagatttt attcatcctg
    67801 aataaacatg aacgggcata cagacgcctg atccccgtat atattttttt agctatagta
    67861 ttatggattt tacctattct cctgattata tacctgtatt tctgtatagt tgacaatgag
    67921 tttctcagaa tcggtttctg tattgcatgg acagcatact actttctttc gcttttcaag
    67981 tattttgtaa aatacttctt tcctctcgat cgtgaagatt tgcttaacct gatggatctc
    68041 gccaaagatt ctcctgatgc gaaacaggag cttcttgagc gtcttttgtc cggaaaaaga
    68101 atgaccgggc gggatgaaat ggatatcaag cgcattctaa aaacaaagga agcacttgat
    68161 aacatcagga attttattga taaacagcag ttaacgaata atgatactcc gaagaaaagc
    68221 acggtcccaa atgaagataa aaaaggcata agatgatgat cacaggaaat ggaataaaca
    68281 ctgtaactgt caatggaaaa gtgaaacaca tcacagaact ggattatata acactttgtt
    68341 tagagtggac aaaactacga gaggaaaaca atcgtcttta tgaaattaat aacaaggcaa
    68401 acagaggatg gcgtggtttc atcctaagat tgataggggt taacctgcca gataaaagga
    68461 ctgagtttac acaacgtctc ttgttgacgc gcaaaatttc gggttcggtc atgaaaaagt
    68521 aacatattct aggctggaac agaccaattt tgcattcgcc gtccaggagt ttgaggggaa
    68581 agtagtcagt gttttacgct cagtcaggga aagtgtgcaa tgataaatga cacgaaagga
    68641 atcgtagtac atgggcaccg cgattcaata ttcaaggaaa aaacaataca gcaatgtact
    68701 tgttctgttt ctattattct ggcgatttca ataaaggcac ttgctcgcgc tctgtcattt
    68761 tctgaaactc ttcatgctgc atttcgattt tccttctaag atcataactc gcatacctgt
    68821 tctttttatg tatatctata tctatttttg tgacgagttc aaaccacaat gttcgcactc
    68881 cccatgcagc gttatgaaac atttccgcaa cggctataga gtctgtatta actgcttcag
    68941 tcatgtagtc gctaatacgt tcagcggtaa ttttcgttat ttcatcgtaa ttcattttat
    69001 ctcctacaag attatttaat ggaatgcaaa atatctggcc tgataataat gttccagaat
    69061 taatatctgc aggctatgtt aagttgtccc cacacacaat ctacctgttt tctcgtgtgt
    69121 gccacaggcg caatacataa atagtctggc cagtaagttc gtaacggatt tcgtactcgc
    69181 cagcaaaaat ccgtctgacc tccctgggtt caaactgaaa taactgttcc cccatacgtg
    69241 gatgagttaa cagaatgacc ggagcctgtg tcagggactg caccgttctg gcggccgcag
    69301 gtttactggc cagcaccaga aaatcatata accgcgccaa atcagaaagc gccttactgg
    69361 tccacttcag ttccatcagc gtggcaccgg taacggattg tcagtactga ggctgtccgc
    69421 ccaggcctgt acagcctgat ggtcgataac ctgtccggat gtcacatcgt ccagggcttc
    69481 cagcgtcagg cgattacgat cctcctcctg ggcaagccat gcagaaagcg cctgtttgat
    69541 aatccagccc cgggagcgtt ccagtctggc ggccatctgg tcgactttat cagccatagg
    69601 taatggcaca tgcgcggtaa ttacttttgt tgcctgtgcg gtattgtttt tcatttgcat
    69661 atccgattag ctgttaatca tatttaatca tagtgattac tttcagacga aacaaggcaa
    69721 ataatgcgcg tcagcgcatg taaggcggca ctccgttgtt acatatggca cagaaacaaa
    69781 accgccgaag cccggcgtca gccggtactg attgatagat ttcaccttac ccatccccat
    69841 cctgctaagc catacccgct ttcagccaag aaatagattc tgtgcgcggt cggagtggtc
    69901 ccgacaaggg tttacccgca gtcggggcgt atctccgcgt tagcgggccg tcagggccgc
    69961 ttacgagcgt gtactgagga cttccagcca gaagactggc agcgatgaca atgtagttac
    70021 aacattcata attaaaagcg atgttgttcc cgcccttcgg gcgggggcgg cttcgctttt
    70081 cagttatgtg gggtgggcac ggtgctgcgt ttgtgtggtg ttttttattc ctgatgtttt
    70141 cgcggtggtt tcagaacggt gaggttcggg gcccgcgcgt tagcgtggga ggtgctttta
    70201 gattctgagc ggcgaggcct tggccgctgc aagcgaaggc ctctttgtat ttgttcacag
    70261 tggtttcaga gataaagact ttccctgcgg cgaagcaacg caggggatta aataatgggg
    70321 ttaggactat attgcaggtt tgctgtttca ggtttgcagc tttttaaagt tctttgtttg
    70381 cggatttatg tggataactg aaacaacgtg gccatgttct gatgactgat acgtggtttt
    70441 atgggcagat atgcccctct gaggggattt aaggtgatta ctgcagattt tgggctgagc
    70501 ggaatcagcg ccgaaagccc cggaggggct ggcgatgatt cagtgctgaa ggaatggtgc
    70561 tgtctagtgc ggcggtggcg cagccagcgt cagttcaagc tgacgcaact ccctgatggc
    70621 cagtcgcttc agatggtcat cggaacaaaa ataggcttca tccggcggca gacgcttcct
    70681 gagatactct gccatctcat gaatctgctg gtcggcaggc agtttcttca gacgtctggc
    70741 gcgcttactg gctgccgctt ttgcacgacg gtgtttcagt gcatcctggc tgcgctgcag
    70801 ataccagcgt tttctggccg catgtacgga gatatcttca tcctcacgca gtacgccttc
    70861 ccggatgagt tgctggcgga tttcactttc acgcaaccgc ttctgctgct gttcgtgaag
    70921 tttcaccatg tcgacgccca gcatctgcca gcctgccggt gttatccaga cgtaacgtgg
    70981 cagacgctgg cgggtttcac ggtcccacag ggtttcctct gaaacaccca gcacaccaaa
    71041 acgtacctgt tcggccagca aacgggaaag gcgggagacc gtcacttcca gttccggaat
    71101 aaccttcccc ttgctgtctt tcgggctgat ttctctggcc agacgggaaa cactcatgcc
    71161 tacggtatgt gtgcccgcat cactgaagct gaccagaacc ggccagacgg catcgaggag
    71221 gcgttgtttt tccggacgga aagcatattt gcgaccggca tacgttcgtt tctgcacgta
    71281 aaaaggatgc agagacatgt gcatgcgcag gctctgctcg ccggtcaccg ggtccttttt
    71341 caccagacgg ttgtaggcgt gcccgagctg cccgctcagc gccttatagc gctcaggacg
    71401 ataccaggac ggattttcgc atttgggttt ggtggagtgc tcaccacgac gacgcttcgt
    71461 gcgcttcctt tccggttcaa ccaggaaagt ggtcgccctt cccgcttcat agtcttccca
    71521 gaaacggatc tgctcttcgg gtgctaactg acaccagtgt actgcgttat atggagtatt
    71581 cttcagacct gccacaagct gtcgcttatg gcggagcgat tgtgccgtgt atttatacac
    71641 tgcacagggt tgaatctctg gtacggtttc attatacttt acccgtattc tgtgaggccc
    71701 ccactatctt tcgtcgttcc gccaagttcg taagaaagat agtgggggtt ttccttttat
    71761 ggcttccgga acatcttcag tcttctgact tactgcccgt acatgccatc tctcctgaat
    71821 gccttatggc gcattctgcc gtccgacttt cgccgctttg ctccacagag tgccaccctc
    71881 tgcacagcta ataacagccg aaatataccc gctttacgtt aatttgaaaa gcattacaac
    71941 gatctcttct cttccagtgc ctcctcactg atacctctaa taagttaatc ttgacctgcg
    72001 tcaaaaaaat aaaatctatt tttaaaagca gatttttctg ctttagatcc ttcttttgga
    72061 ttaaaagatc gacttttgtc ctgctcacga tcatttatct tacaaccgat ccacctaaca
    72121 gaataatatt ctgttttaat gcaattaaac gatctaaaaa acctttaaaa aatcattttg
    72181 tagatcattc gatcgtaaat agaataattc ttagatgata aaaccatttg ttacacaacc
    72241 cgccaattgt gtaacaacag cctctacaag agcatatttg ttcatatgtt cgtcagaact
    72301 gcggattgtc tggacgaggg tcagacaccg tttcgtgcat atggaccagc atgttcgaac
    72361 gccacaaagc agaagcatca cgcctggatc gcttcggcgc ggagcgttaa gttttgacgc
    72421 ttttttgtat gttttttaag ctgttagttg actcggtgcg ttatactaag taccctttac
    72481 tggtcacagg caaaactgtg atcgggcgta ggaacccgcg tagtcatcgt cggcgcagaa
    72541 gttaatttct tcatttggcg tcgttttttg ccataccata aagacaggaa cttgtatttc
    72601 ttgtcgtcat ctatggtagc tcaggcgggg caggcattag cctggccggt ttccgatgac
    72661 gccggtattc ctacccccgt ctgggctatc accaatgagc gtaggaactc agtgatagcg
    72721 tttttagttg tcatcggaga cctttgctat gtcacctcaa ctgatacaga aactcccggc
    72781 catcactctg ttggaaggca tgtttcccga gctatcaaca aaccagctta aagtctgcgt
    72841 tttttacgca atgggtgttc cttacgacgc cattgctcag aactgtcggc tttctcccga
    72901 aacggtacga acctacctga agcgaagcct gaagaactta aatctggaag gatatgatgc
    72961 acttcgctct gctgtgctga tgcgaacgtt cgttttcatg attagcaata cagctaaaga
    73021 aaacgaaaaa atgtgaacag gtgtccccat taaaggggac actttcatgg aaattgcatt
    73081 ttgcacttta caggcgatcc caaattgata aacataaaca ccaagcacaa gttactttaa
    73141 taaagcaaat cacttcagac ggtgtcccaa ttatttgtat ctggctcatg cccgatatat
    73201 ggcgacctta ctctgaactg acgtgctgat gttttatttc gcgtatcaca atactgtgcc
    73261 actgtattgc tgatattagt gcttagccgt tctttatctg tgagttcgcc aaatcaccga
    73321 tacagaacgt actagagtct gctggttata tgcctgtcag aactgcagtt tatccagata
    73381 tgggctggac actgcttcag acatatgatt tagcatattc gcctgacatc gcaaaatcgt
    73441 ccatcatgga tcggttttgc gcggagcgtt gaacttcgtg aatatagaac acctgaataa
    73501 ccggaactgg tatcttgcgc aatataatac agccggcaaa aacagagaat ctttattctc
    73561 atggctgaat gagcaaaacg tcgtaccatg gactccatta ataacaagaa agataagacg
    73621 ggcagactcc aggtgctgtt accgtgaaag aatattcgct atttttccag ggtacttttt
    73681 tattctggct aattttgaca ttcaaccagt atcggcatta cgtcgccata gcgccttcat
    73741 tgatttcgtg aagtttggcg gtgaaataaa acccgtcaat aaagatattg ttgatggatt
    73801 aatgaagatt tacccggacc ctgtgctaaa tccgggagcc agggaagagc tgaatgcggc
    73861 ttccagcata tggctgacaa aagcacaata tcagtatctc ctcagaatgg aaaatactct
    73921 ccagcctgag tcgaggatat ccctgctcct ggaactggta tcaaatgccg aacatcacgg
    73981 ctttatggag cgccttgtaa acatcccata gccagtattt cccggccttc gggccgggct
    74041 tttttcaatt caagagaata ttccggagtg aagcctgcac gaagccatcc ccaccatgga
    74101 ctccccctgt cttttgatgg aaccgtgtat ttcttaatgg cgaaagaaat aaaaggcttc
    74161 accccagagt attgattcgt tttatcaaag gccggcccgt gccatcacgt gtggcggaga
    74221 gaacggatac cggtcttctt ttcatctcac agtaggacat tcgcatgacc acgacagaac
    74281 atcccgtgcg ccagttgctc tggtgtgcac tgaatgccct gaaaacggca caggaaaacc
    74341 acaatataac cagtgaaacc ggattacggc attacctgct tgaatggctt tctggtgcca
    74401 gcagacatcc tgagttccgt agtctccctg gcgaaatcat ggcgttaaaa gcattgaccg
    74461 aaaaagatcg caatattccc atcatcggaa cgctgaatac gctctttctc agctctgcca
    74521 ctgtcgggga atgtccgttg tttcgttttc gggctgcgct gggtaaatta cagaaagcag
    74581 gctggcgcac ttatgtctgc ccatggcctg agagggtgtt caatgaatcc attgaacggg
    74641 cctgttccgg gaaacgccat ctgctacaac taagtcgcac cgaagagtgt tttctaccga
    74701 caggcgatat gagggcacca gttacgttgc agctgatcac tccactacac cgcaaaacta
    74761 tgcagcttct ggaaacggca gagttaatat tctccgacga aggatttcag gtggtgagag
    74821 gatacgaaca tcaattcggg atcgaacgca gagaaaccct ctttcacacc ctatttatcg
    74881 ggctgcccag cttgccagaa gatgtctggg gtgcaacaca ggagcagacg actatcacat
    74941 tacactgagg atgtgtaatc cccggcatac cccgtttcca gtcttcgcag cctgtaccgc
    75001 ccttcctgcc gtttccgaca ttctttctgt cactactcgc ttgtgccgtt ttttcgcaac
    75061 cggtacgtgc agttatgacg gtacagctgc ttcttttccc gttccggaag gagtctttga
    75121 tgccacagca acatcccaga cgactgcaaa tactggtcgt cgacgcacac tgcaaacgca
    75181 ggctgttttc aacgaaaaca ccgacagacc ccgatgagct tgcccgacgt ttctgcacgc
    75241 cggataactg tctcgtggtg gtactacgcg acaaccgatt tctgttccga ctggagcgtg
    75301 ctcccggcag ccactgccgc tggcacaagg gcagcagctc ccgccatcag catctacagg
    75361 actggctatc atgaacgcaa ccgaagcaaa atgtttattc aatggtgtgt gatgatcagg
    75421 aactgcattt ttttgttcca aagaattaaa cagttaatcc tccttagtct gttattttag
    75481 tcgcctgcct cagccaacat gaaaaataat aacgtaccat tatatctctg gttgctttgg
    75541 tgtacgcttt gtctgtacat cactacccca tggcaatact tacgttatcc atacactgac
    75601 aacttatcat tgccttatat gttatatttc tgtgtggtgg ttgtgatttg cattttccca
    75661 ctacttttca gatgtgtact tcttattgta aggtctgcat ctaaatacga cttcacattc
    75721 tttgtgaaga agaaaaaggg aagcaagaaa tcaactatac atcttagccc ctatgctgac
    75781 acacgttgcc cgaagtcaat gaggaaacac tgggatgtat tgctttctct gatatcaaaa
    75841 gcaattaata atgatgaaat cattgtcatg cattcacatc tctttactcc agcaagaata
    75901 aataaactta taaagagact gaaatcttat gatttgagtt tttcagttat tacataccca
    75961 cggaaaacaa gtctgtacga aaaattatca attcctttta tatatctctt atttcagtgg
    76021 tctttcccgc atttgcattc ggacggaatg acagtcgtca ttcgtcctct ccagccgcct
    76081 cacatcaatt aacaaactcg tatatacgaa cacggagtct ccgttatgaa aaaaaactgt
    76141 ctgacactta tactgccgtc attattgtcc ggctgtgccg gaatttccgg cggcaataac
    76201 gtggcaccat cagatacgcc tgctctggtt tttgttgacg gacaaatcag tgagagtgct
    76261 ggcgtcctgg cacttacgca acaacgactt tctccaccta aaataactcc gcccccgaga
    76321 gtcagcgtgc cagagacagt aaaaacagca cagccagtaa gcgaatcccg accgttacct
    76381 tcaccaacaa taagtcagaa tttctcccgg ttaatcatta caggcaagcc tggtagcgcc
    76441 cctgtaacac tatcgccgac ggcccgtaac cggaccgtca gtcagtggat aaaaacatta
    76501 ctaccggaag gatggaaatt cagacaagag aatgcagcca cgcccaaact gaataccaaa
    76561 ctggtttcat ggtctgccaa tgatcagtgg acccgttctc tgaaccggtt gctggaagaa
    76621 caacacctct ggggccatct ggactgggat aataaaacgt tgaccgtcac aacatcggct
    76681 actgcaccgg cacaggacac ggcaaccact atctcgccag cagccatggt aaccacatcc
    76741 acttccccgg agtcttcaac gacagcaaat agccagaaca aaccacgaaa cccattcagg
    76801 ggaaacagcg tatctccgtc tacgcccgct gcaatcccga ctccgaccgg ctccacagtc
    76861 aagtctatcc cactcatgac cgggacacct gtcaaaccgg tatctcaggg taaagagtgg
    76921 cgtgcgccgg caggcactac attaagggaa aacattatta agtgggcaga agaaacaaaa
    76981 tgcgaaagca tggcatcaac gcactggatg gtcatatggc cactttctgt aaccgattac
    77041 cgtcttgatg cgcctttgat gttccgggga tcatttgaat cagccatgga gcaggtattt
    77101 gagttatatc ggaccgcgca aaaaccttta tacgcacagg cctcccgcat gcagtgcatc
    77161 attacagttt ctgatacaag ggatggtcgc taatgggatg gctggtcatg gccgtcggcc
    77221 tgaccattct gattatcact ggaagctacc agaatcagca gatgtctgaa accacgaacg
    77281 cccagcaata tgcaagtgcg tctgtatggg caagtcagat actaatgatt gctaacagaa
    77341 tcaatgatat cagatacgta tcaggccagc aggacggagt aatttcttca gataaactgg
    77401 ctctccctgt cactccggac tcccgaatta aacatcagtt acaacaggga cgtctgtggg
    77461 tatggatgcc tgaacaacca ggacttgttg aaactttgcg ttcaaaaagt cgtggttctg
    77521 cactaatagg gatcttccaa aacgggcaac tcacctggct atcaggaact gcaaccggtc
    77581 tgacaccccc ggcaggcatt actgctggtt ctgtcgtcta cgttaactaa tctggatata
    77641 ttcatgaaaa aatcacacca gcgttcaatg aagctggcgg tgctcccctg catgatcgcc
    77701 gtcgctcttt ccatctccgg atgtactttc agcgaaatca ataaaatgca gaaaaaagca
    77761 caggaggact cagcacatgc acgggaaaag gtatcagccc tttctgcccg caaatcacag
    77821 gctcttacct ggctcgataa tcaatggata aaccctgttc cggtcgctca ggtatcaaga
    77881 gagaaaaaac aaacagctcc ggcctgctac atcacgcagg caagaaaagg agagatcact
    77941 ctacaggaac tggggcaacg tattactgcc gtatgcggca tccccgtaat catcacccct
    78001 gacgcagcca attcaactct tgaaggaggc gctacccgcc agatgactgg gacactacca
    78061 gcacctgatg aaaatggtcg tctgccgtta agcagcctgg gcagcacaac aatgactacc
    78121 tccactcagc cattaacgct gaataacctc atgtggcagg gagatatcaa tggtcttctg
    78181 gatttgatgg ccagccgaag cggtctgtac tggcgcatgg ataatggtcg gattgtattc
    78241 tatctgactg aaaccagaac gtatccactt catatgctga acaccaaaac cagcagcagt
    78301 tccagcgtca gctctggctc aacaagcaca atgggggcaa caggaggcca ggataactca
    78361 gcatccggtg atgctacgtc ctctcagagc acaaccgtcg gtcaggaata cgacctgtat
    78421 gaagacatcc ggaaaactat tgaagcgatg ctgacaccag aaaaaggccg ttactggtta
    78481 tctgcatcga gctcaacgct aacagtcact gatactcctg ctgtccagga agccgtcgca
    78541 cgatatgtgg acgaacaaaa cagtattatg aaccgccagg tagccctgaa cgtacaggtt
    78601 ctgagcgtca gcaataccag aaacgagcag ttcggtctgg actggaacct tgtttacaaa
    78661 tcgctacatt ccgccggagc aacgttgaac aatgcaagcg gagattttac aggcgctaca
    78721 tctgcaggcg tatcaattct ggatacggca acagggaatg ccgccaaatt cagcggttcc
    78781 agccttctga ttaaagcact gagtgagcaa ggcgatgtca gcgttgtaac ttcacaagaa
    78841 agcacggtca caaacctgac gccggtacct atccagatgg cagatcagac agtttacgtc
    78901 gcccagtcag caacaactac gactacggat gtaggagcaa caacaacatt aacaccgggc
    78961 atgatcacca ctggattcaa tatgaccctg ctgcctttaa ttcagaaaac aggcaatctc
    79021 cagttgcaga tgaactttaa tctgtctgat cccccaacta tccgtagctt tacgtcaaaa
    79081 gacggaaaca gttacatcga aatgccatat accaaactgc gttcactgag ccagaaggtc
    79141 aatctgaaag aagggcaatc acttgtcgtt actggtttcg atcagaacaa tacgacgaca
    79201 agtaaagccg gtacgtttac gccagcaaat ccattatttg gtggttcaca aaccgggaaa
    79261 aatgaacgca gcacgcttgt gatcatcatt accccgactt tcccgtcagg aggcaataat
    79321 ggctgatgaa gatatcaacc cggtagtgct tctcgcagat cctaaagtaa atcatcgcgt
    79381 gtgggctgcc tgtcttaaat ggactcctgt cgtaaaaaaa cagcgcgtcc cctcacatca
    79441 taaacacaag tctcatgtaa aacccagacg gcttacctcc ctgaaagtga ctgtaggcag
    79501 cagaaattcc cgtggagaga tcagccgcct taccggcaca ggaatactga ccaggccaga
    79561 acgcaatcat tatttttccc tggcactggc tttctgctca tgggtcagaa atggatacgg
    79621 tgtcttccgg tacagcgata aggagctctt attccttgct tccattaatg gccagcctgc
    79681 cgttatggct gacttatcag gaaatgatgc cgacgttgcc caaaaggtca gcctttttct
    79741 ggcgatgaac gaagagcccc ccgaaaaatg gcaggttgta tcgtctctgg aacatcctga
    79801 taactgggaa agtattatta cccggttatc atcagcagac ctccgccgtt gcaaacttac
    79861 tgtcggcaat cgcagcaaat tcacacttcc tgccgtactg tttttagtag cagccggcgc
    79921 aggcacagta ttctggatga ctcagccaga gcctgatgta ggaccgaccg ccgaggagat
    79981 agctgcacgg gcaagattac agtttaaaaa gcccgaacca ccaccggagc tcccgcatcc
    80041 atgggcgtca caacctgtaa tcagtgattt tctgaaagcc tgcgcggacc ttcgaaaacc
    80101 atcaccggtc gcgctagaag gctggaaact taccggtgga acatgtactc ccgagacatt
    80161 cacactgatt tacgaacgac agccaggagg aactattgaa ggctttctgg caagaagcaa
    80221 agaaatcttt aatgttattc ctgactttaa ccttaaagat ggtgcacgcc tggcttctgt
    80281 aacccggcca ctgccttccc tgccccgcag ggatgaggca gtccctgccc cctcagaaca
    80341 actgatgcgg gtgttcacgt ggtttcagaa aaaacagtta acgccggcca ttaatgaaat
    80401 agccatacag gaaccattac caggtaacga tggtgaacca gctcctgttc agaaatggaa
    80461 agaatatcag ttcagtttat caacgcctgt aaatcctgat gagttgttcc ctttatttca
    80521 ggataccggc gttcgtctga gcaatatcca ttttgaatta aacggcggta ctttcagtta
    80581 ttcctcagaa ggtcatatat atgcgtccag gtaaattact cattattcca tccatatttt
    80641 tcttctctgg tttttcattt gcaacaacac aacctcttgt cacaatagga gaacttgaag
    80701 cacaacaaaa ccggaatatt cttttacagg caaaagttca gggcgcacag ctacagaaac
    80761 aactcgaaga atcagatgtt aatctgtcat cagaaactgt ttcaggtttc tctggtgtga
    80821 ctgcctcctt gccatcagtt tcagagcagc cgacatctaa aaacagaaag gaattgcccg
    80881 ttattatgga aataaacggc aaagataaac gtcttcatgc cgttcttcgc atggctgatg
    80941 gacgtcagac aagcgtcacc actggtagcc agcttccggg tacatccgtc acggtgaagt
    81001 cgatctcatt gtctggcgtc acgttaagtg atggcaccac ccttactttc tgagtgatgt
    81061 gatatggaca aatataatct gaaggatgct ctccttttta tatcccgggg agacacccat
    81121 gaaattctca tcgaaacaaa tcagcgaaca agacctgacg tacagagtaa tctccaggaa
    81181 ctgctaaagt tataccctga catcacccca aaagtagtct cgctgtctga gttacaggaa
    81241 gccggtcagg atgatgaaaa taaaggtccg aaagaacgga ttattcatct gaaagatctt
    81301 gcagatgtca gtgaaagtga gaagaaagtt ctctcctatt ttgaaacggc cagaaaactt
    81361 ggtgcttcgg atattcattt tttaatttca gagtctattt tcaaagttcg catgagaata
    81421 ttcggtgaac tgcagactgt agatgaagat caacctgcat tgggttattc actttgcgca
    81481 acagccattc tgtcaatggc tgatgtcact gaaaccagtt tcttccctca aagggaacag
    81541 gatgcgcgcc tgtccccaca attaatgcga aaaataggaa tatttggtgc acgatacagt
    81601 cacagaccta cgggagatgg tctgattgcg gttatgcgcc tgatacccga tgacggagac
    81661 aaagttccca cgtttaagca actgggcttt ataccggagc aaatccggtt gctgaacgtc
    81721 atgctgcgcc gcccggaagg gaaaattgtt ctgtctggtc caacaggttc gggtaaatca
    81781 acaacgttac gcagtgcctg ccgtgtatat cttgacgata atcaggggcg acacctgctg
    81841 acgatcgaag atccgctgga aggacagata ctgggggcaa cacaaacccc gattatctgt
    81901 gacaaatccg acgaagaggc cgtcaaactg gcatggagtc gtgctatttc atcggcaatg
    81961 cgacttgacc ccgatgccat catggaaggg gaaatgcgcg atcttgtctc gatgatgtca
    82021 accacctacg cggctcagac cggtcacatc gttctgacga cattacatac aaactcagct
    82081 ctgggtatac cggagcgaat gattacgatg ggtatgaatg ccgatcttat ttgtgatgcg
    82141 caattactga ttgggatgat aagccagcgc cttgtaccaa cgctgtgtcc ctcctgccgt
    82201 attccatggg aaaagcgagt acctgaactt agcgaagacg accgcgctta ccttgagcgg
    82261 cactgcaata aagattcact ctgcagtaca gataatatct ggttccgaaa tcctcacgga
    82321 tgctccgact gtaatcacga cgtaattatt aacggcagaa agcgcggtga aataggtaaa
    82381 ggcctcaccg gcaggaccgt aattgctgaa gtgatagaac ctgataatcg cttatttcag
    82441 attctgaaaa cacgcggcaa agttgcagca cgtaaatact ggcttgaaaa catgcatggc
    82501 atcagtcgtg tggagcatct gcttcgccgt atcaatgaag gcctggtgga ccctcttgaa
    82561 gccgacagaa tcatccctct ggacgaagac gaacgcctca gcatcgatga tgcctaacag
    82621 gagaacaaca tgcgtgaaat gaatttcagt caacggctga gacgtttcat tgtcagaaaa
    82681 acgttcagcg ccccctacag ggtgcagttt tacgaagctc tgcgttttct gctggaaaat
    82741 aaacagccat taaagaccgc actggaacag atgcgggatg cctggacaga ctttggacga
    82801 aaatggcacc cctttgctga actggccaca gactgtattg agtctcttcg tgaaaacagt
    82861 ggcgaaaact ctctggaata tactcttggt ttatgggttc cccaggaaga ggctgcagta
    82921 atcagtgccg gaataagaag tggttccatt gtggatgcat tacaatttgc caccacgctt
    82981 acagatgcca aaaagcaact ccatcaggcc atatggcaaa tggctattta cccggtggga
    83041 ttgttaatca tgatgacggg gacattatat gttcttaata cagagctaat acctgtactg
    83101 agcaaaataa gctcccctga ttcatggagt ggtgctcttg gttttctcta tggattatct
    83161 ttgtttgtcg ataattacgg cgctatatgt gccgttcttt ttgcagtggt cacaggtgta
    83221 atatcatggt ctctgaaaaa ctggaaaagc cgctctttgg ctgacaatat aatgccatgg
    83281 agtatttata aggatataca gggggcagca ttcctcctga atatggctgc acttctgaaa
    83341 gccaaaatga caacgctcaa ctcactgaac gttcttcagg aatttgcatc accatggtta
    83401 agtacacgcc ttgacagcat tatttaccgc gtccgccagg gggatcacct tgggctggct
    83461 ttacgacagt gcggttatca tttcccttcc agggagtctg ccaactttct gtctttatta
    83521 cagggagatg gtgcaacgga gctaatcggc aattatggcc aacggtggct tgtacaaacg
    83581 ctggagcgtg taaaaaaacg agcagctgtt gtgcgtttaa ttatgctgat atttcttgtc
    83641 atgtcactcc tgttactggt catggccgtt gtagacattc agtctattgg cgacaacagt
    83701 atgggtagta tttaaataaa acgaggtatt tatgttaatt caaaatatta atgctaacgt
    83761 tctgaagaaa aatgagaaag aaacgtctca tgacaaaggc tggggtatta tggaacaggg
    83821 tgcaatagca cttgttgtaa ttatcgtact tgcccttgtt tttggtggat tgtatatgtt
    83881 gcgttcacga accggtgtgg caaatgaaag ttcgaacata cagacaatca tcacaagtac
    83941 tcaggggctg ctaaaaggta gcgatggata tacgtttacc agtggagcca aaatgacggg
    84001 tgctctcatt cagatgggtg gcgtacccaa gaccatgaca gtgcgtggta ctccatcatc
    84061 aggtacagca acgctctata actcatgggg cggtgccgta actgttgctc cggcttcaac
    84121 atcaggtttc aacaatggtt ttaccgtcac ctatgaaaaa gtcccacagg atgcctgtat
    84181 ccagattgcc acacaaatca gtcgtactgg tttaactaac ggtattaccc tgaacagtac
    84241 ggctcacagt gatggaaaag tcactactga agaagccagt gcgcaatgca cagctgataa
    84301 tggcagcacc ggtacaaaca aactaatctt caccatcaat ggctaaggag ttttaatgtc
    84361 cgggggtaaa gtgcccctca gacttatttc attaatcctg ctttgttctc ttatttatcc
    84421 cgcccgagct tcatacatcg cccccaaaca aatctggact aacaagtggg acaattgctt
    84481 tgccgccgcc ggcgcacgct atcaaattga accattattg ttgaaggcca tttctgccgg
    84541 ggaatcttca ttgaagccgg gcgcaattaa cataaataag gacagaaaaa caggcaaagc
    84601 cagtagtacg gattatgggt tgatgcaaat aaactcaacc catattccaa agcttataaa
    84661 gatgggcgta atcaaaaagt ctgaggattt aattaccaaa ccatgtctta atatccatat
    84721 aggaagctgg atactggcaa gacattttca gatatgtggc gtcagctgga attgccttgg
    84781 gtcatacaat gccggattcc gtaaggacag acatgaaaca agagagcaat atgctaataa
    84841 agtatggaga atttatagtg acatgaaagg gatatgcctt cccgggcagg gaggcagaca
    84901 atgcagacaa tcgtaggcat aagtctctcc cctgtttata tcctcccatt gatgttcata
    84961 ttttccatta ttggacgaac tctgttattc agagttcgtt attttttatc tgatacagaa
    85021 catctctggt ataagacaca cactgcagtt ctgtcaggaa tatggctata tagtatagct
    85081 gtggcattaa ttattttatc gtcatcccca cttatctacc gtattcatgc tgttcttatt
    85141 ctttcgttca tgctgcaaat ggcagtaacg gatgcactaa caggcctctt accgggtaca
    85201 ttcaccaggc gttttttaat cgctggaata ttatcacaaa tcacaactga tatatggtgg
    85261 ctccgtgcaa cagaaattgt ctctgccgca attgtcttat tctgtctgca taaactggtt
    85321 aaccgccatc gtcttaatat tggcacagga gacctgtggc ttattgcagg tattaccgca
    85381 tggtcagggc tatataacgc catatggagt gtgttgctgg gcgcaggagg atttgtctta
    85441 tggcattcaa catggtgcat aaaaggacat aaagaaggcc cactgggtcc atggctgtgt
    85501 cttggccatg tccttttgct actggataat ctttatcaac cactatgggt aatctgatga
    85561 aaaaatatga ccgaggctgg gcatcgctgg aaattggcgc ggctttattg attgttatga
    85621 tcatagttgc ctggggtgca ggcatatggc aggactattt aaaaaccaaa ggttggcagg
    85681 ctgaagctcg tcttgtcagc aactgggcca gtgcagcccg ctcttatata gggaaaaact
    85741 acacaactct ccaggccagt agtaccacaa caacccctgc cgttatcacg acaaccatgc
    85801 tgaaaaatac cggctttttg tccagcggct ttactgagac aaacagcgag gggcagcggt
    85861 tacaggcata tgtagttcga aacacccaaa acccggaatt actacaggca atggttgtat
    85921 ccagtggtgg caccccttat ccagtgaaag cacttatcca gatggctaag gatattacca
    85981 ctggtcttgg tggatatatc caggacggca aaacagccac aggtgcatta cgttcctggt
    86041 cagtagcttt aagtaattat ggtgccaaaa gcggtaacgg gcatattgcc gtactgttat
    86101 cgacagatga acttagtggt gcagcagagg acactgatcg tctttacaga ttccaggtca
    86161 atggtcgccc tgacttaaac aaaatgcaca cggccattga tatgggatca aataacctga
    86221 ataacattgg ggcagtaaat gcccagactg gtaatttcag cggcaatgtg aatggtgtaa
    86281 atggcacttt cagcggtcag gtaaaaggca atagcggaaa ctttgacgta aatgtcaccg
    86341 ctggcggtga tatcagaagt aataatggtt ggttaattac tcgtaacagc aaaggctggc
    86401 tcaatgaaac tcacggtggc ggattttata tgtccgacgg atcatgggtt cgaagtgtaa
    86461 acaacaaggg catctatacc ggcggtcagg tgaaaggcgg tactgttcgt gctgatggtc
    86521 gcctttatac tggtgaatac ttacaactgg aaagaactgc cgttgctggc gcatcatgtt
    86581 cgcctaacgg ccttgtaggc cgcgataata caggggca
//
LOCUS       DAJVXP010000003        79576 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVXP010000003 DAJVXP010000000
VERSION     DAJVXP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.003"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            93..1195
                     /gene="bluF"
                     /locus_tag="OQS10_000187"
                     /pseudo
     CDS             93..1195
                     /gene="bluF"
                     /locus_tag="OQS10_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1400..2131
                     /gene="bluR"
                     /locus_tag="OQS10_000188"
     CDS             1400..2131
                     /gene="bluR"
                     /locus_tag="OQS10_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCS2291329.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2352..2756
                     /locus_tag="OQS10_000189"
     CDS             2352..2756
                     /locus_tag="OQS10_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCS2291330.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2809..2919
                     /locus_tag="OQS10_000190"
     CDS             2809..2919
                     /locus_tag="OQS10_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291331.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3155..3199
                     /locus_tag="OQS10_000191"
     CDS             3155..3199
                     /locus_tag="OQS10_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291332.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3452..3775
                     /gene="iraM"
                     /locus_tag="OQS10_000192"
     CDS             3452..3775
                     /gene="iraM"
                     /locus_tag="OQS10_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS2291333.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3878..5128)
                     /gene="icd"
                     /locus_tag="OQS10_000193"
     CDS             complement(3878..5128)
                     /gene="icd"
                     /locus_tag="OQS10_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS2291334.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVIDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5300..5953
                     /gene="rluE"
                     /locus_tag="OQS10_000194"
     CDS             5300..5953
                     /gene="rluE"
                     /locus_tag="OQS10_000194"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS2291335.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5963..6424
                     /gene="nudJ"
                     /locus_tag="OQS10_000195"
     CDS             5963..6424
                     /gene="nudJ"
                     /locus_tag="OQS10_000195"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS2291336.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6478..7584
                     /gene="mnmA"
                     /locus_tag="OQS10_000196"
     CDS             6478..7584
                     /gene="mnmA"
                     /locus_tag="OQS10_000196"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS2291337.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7620..8261
                     /gene="hflD"
                     /locus_tag="OQS10_000197"
     CDS             7620..8261
                     /gene="hflD"
                     /locus_tag="OQS10_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS2291338.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8265..9635
                     /gene="purB"
                     /locus_tag="OQS10_000198"
     CDS             8265..9635
                     /gene="purB"
                     /locus_tag="OQS10_000198"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS2291339.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9803..10474
                     /gene="phoP"
                     /locus_tag="OQS10_000199"
     CDS             9803..10474
                     /gene="phoP"
                     /locus_tag="OQS10_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS2291340.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10474..11934
                     /gene="phoQ"
                     /locus_tag="OQS10_000200"
     CDS             10474..11934
                     /gene="phoQ"
                     /locus_tag="OQS10_000200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS2291341.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12010..13131
                     /gene="roxA"
                     /locus_tag="OQS10_000201"
                     /gene_synonym="ycfD"
     CDS             12010..13131
                     /gene="roxA"
                     /locus_tag="OQS10_000201"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS2291342.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13180..14406)
                     /gene="pepT"
                     /locus_tag="OQS10_000202"
     CDS             complement(13180..14406)
                     /gene="pepT"
                     /locus_tag="OQS10_000202"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS2291343.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14656..15792
                     /gene="potA"
                     /locus_tag="OQS10_000203"
     CDS             14656..15792
                     /gene="potA"
                     /locus_tag="OQS10_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS2291344.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15776..16633
                     /gene="potB"
                     /locus_tag="OQS10_000204"
     CDS             15776..16633
                     /gene="potB"
                     /locus_tag="OQS10_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS2291345.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16630..17424
                     /gene="potC"
                     /locus_tag="OQS10_000205"
     CDS             16630..17424
                     /gene="potC"
                     /locus_tag="OQS10_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS2291346.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17421..18467
                     /gene="potD"
                     /locus_tag="OQS10_000206"
     CDS             17421..18467
                     /gene="potD"
                     /locus_tag="OQS10_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS2291347.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18623..19444)
                     /gene="cobB"
                     /locus_tag="OQS10_000207"
     CDS             complement(18623..19444)
                     /gene="cobB"
                     /locus_tag="OQS10_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS2291348.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19460..20371)
                     /gene="nagK"
                     /locus_tag="OQS10_000208"
     CDS             complement(19460..20371)
                     /gene="nagK"
                     /locus_tag="OQS10_000208"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS2291349.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20400..21644)
                     /gene="lolE"
                     /locus_tag="OQS10_000209"
     CDS             complement(20400..21644)
                     /gene="lolE"
                     /locus_tag="OQS10_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS2291350.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21644..22345)
                     /gene="lolD"
                     /locus_tag="OQS10_000210"
     CDS             complement(21644..22345)
                     /gene="lolD"
                     /locus_tag="OQS10_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS2291351.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22338..23537)
                     /gene="lolC"
                     /locus_tag="OQS10_000211"
     CDS             complement(22338..23537)
                     /gene="lolC"
                     /locus_tag="OQS10_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS2291352.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23799..24872
                     /locus_tag="OQS10_000212"
     CDS             23799..24872
                     /locus_tag="OQS10_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS2291353.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25000..28446
                     /gene="mfd"
                     /locus_tag="OQS10_000213"
     CDS             25000..28446
                     /gene="mfd"
                     /locus_tag="OQS10_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS2291354.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28590..29546
                     /gene="ldtC"
                     /locus_tag="OQS10_000214"
                     /pseudo
     CDS             28590..29546
                     /gene="ldtC"
                     /locus_tag="OQS10_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29629..29886)
                     /gene="bhsA"
                     /locus_tag="OQS10_000215"
     CDS             complement(29629..29886)
                     /gene="bhsA"
                     /locus_tag="OQS10_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS2291355.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30127..30759
                     /gene="comR"
                     /locus_tag="OQS10_000216"
     CDS             30127..30759
                     /gene="comR"
                     /locus_tag="OQS10_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS2291356.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30821..31360)
                     /locus_tag="OQS10_000217"
     CDS             complement(30821..31360)
                     /locus_tag="OQS10_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS2291357.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31587..32891)
                     /gene="ndh"
                     /locus_tag="OQS10_000218"
     CDS             complement(31587..32891)
                     /gene="ndh"
                     /locus_tag="OQS10_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS2291358.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33299..33841)
                     /locus_tag="OQS10_000219"
     CDS             complement(33299..33841)
                     /locus_tag="OQS10_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2291359.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33864..34889)
                     /gene="nagZ"
                     /locus_tag="OQS10_000220"
     CDS             complement(33864..34889)
                     /gene="nagZ"
                     /locus_tag="OQS10_000220"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS2291360.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34900..35724)
                     /gene="thiK"
                     /locus_tag="OQS10_000221"
     CDS             complement(34900..35724)
                     /gene="thiK"
                     /locus_tag="OQS10_000221"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS2291361.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35705..36346)
                     /gene="lpoB"
                     /locus_tag="OQS10_000222"
     CDS             complement(35705..36346)
                     /gene="lpoB"
                     /locus_tag="OQS10_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS2291362.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36360..36737)
                     /locus_tag="OQS10_000223"
     CDS             complement(36360..36737)
                     /locus_tag="OQS10_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS2291363.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36740..37099)
                     /gene="hinT"
                     /locus_tag="OQS10_000224"
     CDS             complement(36740..37099)
                     /gene="hinT"
                     /locus_tag="OQS10_000224"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS2291364.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37433..39622
                     /gene="fhuE"
                     /locus_tag="OQS10_000225"
     CDS             37433..39622
                     /gene="fhuE"
                     /locus_tag="OQS10_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCS2291365.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(39682..41115)
                     /gene="ptsG"
                     /locus_tag="OQS10_000226"
     CDS             complement(39682..41115)
                     /gene="ptsG"
                     /locus_tag="OQS10_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS2291366.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41410..42207)
                     /locus_tag="OQS10_000227"
     CDS             complement(41410..42207)
                     /locus_tag="OQS10_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS2291367.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42218..43222)
                     /gene="holB"
                     /locus_tag="OQS10_000228"
     CDS             complement(42218..43222)
                     /gene="holB"
                     /locus_tag="OQS10_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS2291368.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43219..43860)
                     /locus_tag="OQS10_000229"
     CDS             complement(43219..43860)
                     /locus_tag="OQS10_000229"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS2291369.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43850..44872)
                     /gene="yceG"
                     /locus_tag="OQS10_000230"
     CDS             complement(43850..44872)
                     /gene="yceG"
                     /locus_tag="OQS10_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS2291370.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44875..45684)
                     /gene="pabC"
                     /locus_tag="OQS10_000231"
     CDS             complement(44875..45684)
                     /gene="pabC"
                     /locus_tag="OQS10_000231"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS2291371.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45804..47045)
                     /gene="fabF"
                     /locus_tag="OQS10_000232"
     CDS             complement(45804..47045)
                     /gene="fabF"
                     /locus_tag="OQS10_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS2291372.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47133..47369)
                     /gene="acpP"
                     /locus_tag="OQS10_000233"
     CDS             complement(47133..47369)
                     /gene="acpP"
                     /locus_tag="OQS10_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS2291373.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47580..48314)
                     /gene="fabG"
                     /locus_tag="OQS10_000234"
     CDS             complement(47580..48314)
                     /gene="fabG"
                     /locus_tag="OQS10_000234"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS2291374.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48327..49256)
                     /gene="fabD"
                     /locus_tag="OQS10_000235"
     CDS             complement(48327..49256)
                     /gene="fabD"
                     /locus_tag="OQS10_000235"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS2291375.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49272..50225)
                     /gene="fabH"
                     /locus_tag="OQS10_000236"
     CDS             complement(49272..50225)
                     /gene="fabH"
                     /locus_tag="OQS10_000236"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS2291376.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50293..51363)
                     /gene="plsX"
                     /locus_tag="OQS10_000237"
     CDS             complement(50293..51363)
                     /gene="plsX"
                     /locus_tag="OQS10_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS2291377.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51444..51617)
                     /gene="rpmF"
                     /locus_tag="OQS10_000238"
     CDS             complement(51444..51617)
                     /gene="rpmF"
                     /locus_tag="OQS10_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS2291378.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51669..52190)
                     /gene="yceD"
                     /locus_tag="OQS10_000239"
     CDS             complement(51669..52190)
                     /gene="yceD"
                     /locus_tag="OQS10_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS2291379.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52389..52973
                     /gene="yceF"
                     /locus_tag="OQS10_000240"
     CDS             52389..52973
                     /gene="yceF"
                     /locus_tag="OQS10_000240"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS2291380.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53085..54044)
                     /gene="rluC"
                     /locus_tag="OQS10_000241"
     CDS             complement(53085..54044)
                     /gene="rluC"
                     /locus_tag="OQS10_000241"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS2291381.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54617..57802
                     /gene="rne"
                     /locus_tag="OQS10_000242"
     CDS             54617..57802
                     /gene="rne"
                     /locus_tag="OQS10_000242"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS2291382.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57997..58950)
                     /gene="flgL"
                     /locus_tag="OQS10_000243"
     CDS             complement(57997..58950)
                     /gene="flgL"
                     /locus_tag="OQS10_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS2291383.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58962..60605)
                     /gene="flgK"
                     /locus_tag="OQS10_000244"
     CDS             complement(58962..60605)
                     /gene="flgK"
                     /locus_tag="OQS10_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS2291384.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60671..61612)
                     /gene="flgJ"
                     /locus_tag="OQS10_000245"
     CDS             complement(60671..61612)
                     /gene="flgJ"
                     /locus_tag="OQS10_000245"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS2291385.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61612..62709)
                     /gene="flgI"
                     /locus_tag="OQS10_000246"
     CDS             complement(61612..62709)
                     /gene="flgI"
                     /locus_tag="OQS10_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS2291386.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62721..63419)
                     /gene="flgH"
                     /locus_tag="OQS10_000247"
     CDS             complement(62721..63419)
                     /gene="flgH"
                     /locus_tag="OQS10_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS2291387.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63472..64244)
                     /gene="flgG"
                     /locus_tag="OQS10_000248"
                     /pseudo
     CDS             complement(63472..64244)
                     /gene="flgG"
                     /locus_tag="OQS10_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64416..65171)
                     /gene="flgF"
                     /locus_tag="OQS10_000249"
     CDS             complement(64416..65171)
                     /gene="flgF"
                     /locus_tag="OQS10_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS2291388.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65191..66396)
                     /gene="flgE"
                     /locus_tag="OQS10_000250"
     CDS             complement(65191..66396)
                     /gene="flgE"
                     /locus_tag="OQS10_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS2291389.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66421..67116)
                     /gene="flgD"
                     /locus_tag="OQS10_000251"
     CDS             complement(66421..67116)
                     /gene="flgD"
                     /locus_tag="OQS10_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS2291390.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67128..67532)
                     /gene="flgC"
                     /locus_tag="OQS10_000252"
     CDS             complement(67128..67532)
                     /gene="flgC"
                     /locus_tag="OQS10_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS2291391.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67536..67952)
                     /gene="flgB"
                     /locus_tag="OQS10_000253"
     CDS             complement(67536..67952)
                     /gene="flgB"
                     /locus_tag="OQS10_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS2291392.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68107..68766
                     /gene="flgA"
                     /locus_tag="OQS10_000254"
     CDS             68107..68766
                     /gene="flgA"
                     /locus_tag="OQS10_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS2291393.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68842..69135
                     /gene="flgM"
                     /locus_tag="OQS10_000255"
     CDS             68842..69135
                     /gene="flgM"
                     /locus_tag="OQS10_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS2291394.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69140..69556
                     /gene="flgN"
                     /locus_tag="OQS10_000256"
     CDS             69140..69556
                     /gene="flgN"
                     /locus_tag="OQS10_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS2291395.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69596..71131)
                     /gene="murJ"
                     /locus_tag="OQS10_000257"
     CDS             complement(69596..71131)
                     /gene="murJ"
                     /locus_tag="OQS10_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS2291396.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71241..72164)
                     /locus_tag="OQS10_000258"
     CDS             complement(71241..72164)
                     /locus_tag="OQS10_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2291397.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72166..72813)
                     /locus_tag="OQS10_000259"
     CDS             complement(72166..72813)
                     /locus_tag="OQS10_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS2291398.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72824..73408)
                     /gene="rimJ"
                     /locus_tag="OQS10_000260"
     CDS             complement(72824..73408)
                     /gene="rimJ"
                     /locus_tag="OQS10_000260"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS2291399.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73644..74852
                     /gene="mdtH"
                     /locus_tag="OQS10_000261"
     CDS             73644..74852
                     /gene="mdtH"
                     /locus_tag="OQS10_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS2291400.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74916..75563
                     /gene="grxB"
                     /locus_tag="OQS10_000262"
     CDS             74916..75563
                     /gene="grxB"
                     /locus_tag="OQS10_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS2291401.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75697..76257
                     /locus_tag="OQS10_000263"
     CDS             75697..76257
                     /locus_tag="OQS10_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2291402.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76363..77409
                     /gene="pyrC"
                     /locus_tag="OQS10_000264"
     CDS             76363..77409
                     /gene="pyrC"
                     /locus_tag="OQS10_000264"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS2291403.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77483..77728
                     /gene="dinI"
                     /locus_tag="OQS10_000265"
     CDS             77483..77728
                     /gene="dinI"
                     /locus_tag="OQS10_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS2291404.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78018..78272
                     /gene="bssS"
                     /locus_tag="OQS10_000266"
     CDS             78018..78272
                     /gene="bssS"
                     /locus_tag="OQS10_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS2291405.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78387..79505
                     /gene="solA"
                     /locus_tag="OQS10_000267"
     CDS             78387..79505
                     /gene="solA"
                     /locus_tag="OQS10_000267"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS2291406.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19592 a        20913 c        19711 g        19360 t
ORIGIN      
        1 tgcttaccac ccttatttat cgtagccata tacgtgacga cgaacctgtc aaaaaaatcg
       61 aagaaatggt ttcgatagca aatcgcagga acatgcagtc tgacgtaaca gggatcttac
      121 tgtttaatgg ttctcatttt ttccagcttc tggaaggtcc ggaagaacag gttaaaatga
      181 tatatcgggc tatatgccag gatccacggc actataatat tgttgagctg ctgtgcgatt
      241 acgcgcctgc tcgccgtttt ggcaaagcgg gaatggaatt atttgatttg cgcctgcacg
      301 agcgagatga cgttttacag gccgtattcg acaaaggcac atcaaaattt cagctaactt
      361 atgatgacag agcgctacaa ttttttcgta cttttgtcct tgcaaccgaa caatcaacct
      421 atttcgagat ccctgccgaa gactcctggc tttttatcgc tgacggatct gataaagaac
      481 ttgattcctg taccctttca ccaactataa acgaccactt tgcctttcat cccttatcgc
      541 ggcggataat cgcttttgaa gccattgtgc aaaaaaatga agcccatcag ccatagcggt
      601 tgggcagcgt aaagacgggg aaatctacac agcggatctc aaaagtaagg cgcttgcatt
      661 cgcgatggca cacgcacttg agctcggtga taaaatgatt tcaatcaatc tattacctat
      721 gaccctggtt aacgaacctg acgcagtctc ttttttactt gatgaaataa aggccaatgc
      781 tctggtgcct gaacaaatca tcgttgaatt tactgaaagt gaagttatat ctcggtttga
      841 tgagtttgca gaagcgatta aatcgcttaa ggctgtcggt atcagtgtag caattgatca
      901 ttttggcgca ggttttgccg gtttgttact cctgtcacgc ttccagcctg acagaattaa
      961 aatcagtcag gaattgatta ccaatgttca taaaagcggg ccacggcagg caattattca
     1021 agcgatcata aaatgctgta catcacttga aattcaagtc agtgctatgg gcgtggcaac
     1081 accagaagag tggatgtggc ttgaatccgc aggaattgag atgtttcagg gagatctgtt
     1141 tgctaaagct aaattgaatg gtatcccttc catagcgtgg ccagagaaaa aataattttc
     1201 agcacattct ttcacatgat ttcagtaaat catcgtgaaa atgaatacgt tatgtttaaa
     1261 tttaatccac attaaatata atgtttcatc caatttagct gaaatcttat ttatactttg
     1321 tacaatttta gcgtatattg tacaaagtat tattgggtcg tgtacaggcg gcggagattt
     1381 atgaccgcaa ggaggaattg tggcttatta cagcattggt gatgttgctg aacgttgcgg
     1441 gattaatcct gtcactctcc gggcctggca acgccgctac ggtttgttaa aaccacagcg
     1501 cagtgaaggc ggacaccgac tctttgatga agaagacata caacgcatcg aagagatcaa
     1561 gcgttggata agtaatggcg tccctgtagg caaagttaaa gcattactgg aaaccaccag
     1621 ccaggatacg gaagatgact ggaaccgcct gcaagaagag atgatgtcaa ttcttcgtat
     1681 ggctaatcct gccaaactac gcgcgagaat tatttcactg ggtcgagagt acccagtaga
     1741 tcaattgatt aatcatgttt accttcctgt tcgccagcgt ctcgtgcttg atcacaacac
     1801 ctcccgcatt atgagcagta tgtttgacgg cgcattaatt gaatatgcag cagcctcact
     1861 tttcgaaatg cgccgtaagc ccggtaaaga agccattctg atggcgtgga atgttgaaga
     1921 gagagcacga ttgtggctgg aagcatggcg tttatcattg tcaggatggc acatttctgt
     1981 tcttgctgat cccattgaat cgccgcaccc ggaactgttc ccgacgcaaa cattgattgt
     2041 ctggacaggc atggcaccaa cgagaaggca aaacgaactt ttacaacatt ggggtgagca
     2101 aggttataaa gtcatctttc atgcccccta atttcacctt tatcccgccc cttagacaga
     2161 atgttataat ctgaacccac aacagcattt gtaccggagc tggtccgttg caaaatgctc
     2221 accggaagaa ttcaggtaat ccctctatca cactctgtac atatcacagc caaaggacag
     2281 accagattga agtggtaaaa ccgatcgtgc acactacgca gtctcacaac taacaagagg
     2341 ttattatcct gatggatcag gtcgttgttt ttcaaaagat gtttgagcaa gtgagaaaag
     2401 agcaaaactt ctcctggttt tattcagaat taaaacatca ccgtattgca cattacattt
     2461 attatctggc tacggataac atcagaatta ttactcacga tgacacggtt ttgttattaa
     2521 gaggaaccag gaacctgtta aaagttagta cgaccaagaa ccctgctaaa ataaaagagg
     2581 ccgcattgct tcatatttac ggaaaatcta catttcggga atactgttca acactggcag
     2641 gcgcgggcgt tttccgttgg gttactgatg ttaatcataa caaacgcagt tactacgcca
     2701 ttgataatac gcttttatat attgaagatg tagaaaataa taaaccatta atctagctaa
     2761 agttggatgc ttaagaaatg cttcataatt cagtaaggca ttagcataat gggaataaaa
     2821 gtacagagac tatccctatg gatgataaat actgtccttt tattgctacc cataaataat
     2881 caccaggcca atactatcaa attgatattt gaaatgtgat cacttgactt tctatacgtt
     2941 attttataac ggttaatata tttataaaaa caacgggtgt gccatacgcc cgtttcaata
     3001 cttaacgcac atgtgttttg gtttagtcat catccagtta tatgtatttt agccaggaac
     3061 aggttaaacc tttcctatat aactcaaaaa ttgaaacctt attctcatgt catgtttata
     3121 ttcattatta tcgttatata aaaaggcaac cataatgttt agcaaattgg caccaagtag
     3181 cataaaggct atgttttaat tacaggatgt tcagtcattt gaatgtataa cattatagct
     3241 aaacaaatct aaaacgaagt gaataattta ttgttttcac taaatctcat tttgtttaac
     3301 atccattgag attccttgct ttaaattttg ttttatataa gcaatcgttt taattaattt
     3361 attttttgaa ggggtaatat actcatatgc aaaataaaga aatgaacatc caaatgaact
     3421 atattaaata ccgtgggata agacataaca aatgaagtgg gtagtaattg acacggtaat
     3481 tcaacctaca tgcggcatat ctttttcagc catatggggt gatatgaaaa agatcatctg
     3541 gtatcaatct actatatttc ttcctcctgg aagtatattt acaccggtta agcttggtat
     3601 tatccttaag gaaaaagaat atcctattac tatttataac attgcaccat tcaacaagaa
     3661 tttatggagt ttactcaaaa gcagtcaaga gtgtcctcca ggagaaagca aaataacaaa
     3721 taaatgttta cataatagtt gcattataaa aatatgccca tatgggctca agtaatgggc
     3781 ttcatataaa tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt
     3841 gttatacgcc cgtttcaata tttaactcat ggagagatta catgttttcg atgatcgctt
     3901 caccaaactc tgaacatttc agcagcttag cgccttccat cagacgttca aagtcataag
     3961 ttacggtttt cgcgttgata gcaccttcca tacctttaac aattaaatca gccgcttcag
     4021 tccaccccat atggcgcagc atcatctcag cggagaggat gatagagcct ggatttactt
     4081 tgtcctgacc ggcatatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt
     4141 caccgatgtt tgcgccaggg gcgataccga taccgccaac ctgcgctgcc agggcgtcag
     4201 aaatgtagtc accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca
     4261 ggatctgttg caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtgt
     4321 tcgggttttt aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aactcttcac
     4381 gcgccagctg gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt
     4441 tgtgcaccag agtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa
     4501 ccagacgttt ggtgccttct tccgaacacg gcttaatacc gataccacaa tgttccggga
     4561 agcgaatttt cttcaccccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg
     4621 cagagtctgc tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa
     4681 ccatatcggt cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg
     4741 gacgcaggca gatgtagaga tccagttcct ggcgcagggc aacgttcaga gagcgaatac
     4801 cgccaccaac aggagtggtc agcggacctt taatggcaac gcgatattca cgaatcagat
     4861 caagggtttc agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg
     4921 tgtaaatttc catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt
     4981 cgatcacttt cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag
     5041 ggataatcgg attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt
     5101 gtgccggaac aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc
     5161 gtctgccacc acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt
     5221 tttgttaatg atttgtaatt ggcttgtcaa tgctacccta ttgtttgccg tcatgaaaga
     5281 tgcgcgttat taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac
     5341 ttcttttaga aatcaccagg ttaagcgatt cagctcgcaa cgttctacta ggcgtaaacc
     5401 tgaaaaccag cccacgcgtg tgatcctgtt caataaaccc tacgatgttc ttccgcagtt
     5461 caccgatgaa gccggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc
     5521 agcaggtcgt cttgaccgcg ataccgaagg gttactggtg ctgaccaata acggcgcgtt
     5581 gcaggcgcgt ttaacccagc cgggcaaacg caccgggaaa atctattatg tgcaggtgga
     5641 aggtattccc acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg
     5701 ccctaccctg cccgccggcg cggagctggt tgacgaacca gcgtggttat ggccgcggaa
     5761 tccaccaatt cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga
     5821 aggacgtaat cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact
     5881 gattcgttat gcgatgggtg attacacttt ggataacctt gccaatggcg aatggcgaga
     5941 agcaacagaa taaggatgca caatgtttaa accgcacgtt accgttgctt gcgtagtgca
     6001 cgcagagggt aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca
     6061 acctgccggg catctggaag ccgatgaaac cttagtggaa gctgccgccc gtgagctatg
     6121 ggaagaaacc ggcatcagcg cgcaaccgca acactttatt cgtatgcatc agtggattgc
     6181 gccagataaa acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc
     6241 gacccagcct catgacagcg atatcgactg ctgtcgttgg gtcagcgccg aagaaatttt
     6301 aaaggcgtca aatcttcgtt cgccgctggt ggcggaaagt atccgttgtt atcaaagtgg
     6361 gcaacgctat ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat
     6421 ctaaaaggct gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg
     6481 tctgaaaccg caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct
     6541 gcctggctgt tgcaacaaca gggatatcag gttgaaggcc tgtttatgaa gaactgggaa
     6601 gaagacgacg gtgaggaata ttgcacagcg gcagcggatc tggctgatgc ccaggctgtc
     6661 tgcgacaagc tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaat
     6721 gtcttcgaac tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg
     6781 tgcaacaaag agatcaaatt taaagctttc ctcgaatttg ccgccgaaga tttaggtgcc
     6841 gattatatcg ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg
     6901 ctgcgtggtc tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag
     6961 cagattgcgc aaagcctgtt cccggtcggc gaactggaaa aaccgcaggt gcgtaagatt
     7021 gctgaagatc ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt
     7081 ggcgaacgta aattccgcga gttcctgggc cgttatctcc cggggcaacc gggcaaaatc
     7141 attaccgtcg atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt
     7201 cagcgtaaag gtctgggtat cggtggcacc aaagaaggca ccgaagaacc ttggtatgtg
     7261 gtggacaaag acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg
     7321 ctgatgtctg tcgggttgat tgcccagcag ttgcactggg tcgatcgcga accattcacc
     7381 ggcactatgc gttgcacggt aaaaacccgc tatcgccaga ccgatatccc ttgcaccgtc
     7441 aaggcgctgg acgatgatcg cattgaagtg attttcgatg aaccggttgc cgccgtgacg
     7501 ccgggccagt ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag
     7561 cagcgtctgc cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg
     7621 tggcaaagaa ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc
     7681 tggtgcaaca actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca
     7741 acagtattat tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca
     7801 acctgcgcgt cgggctggaa accctgctcg gcgtgctcaa tgccagcagt cgccagggct
     7861 taaacgccga attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct
     7921 cagcgaaagg cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg
     7981 aacacttcga tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg
     8041 tgatcagccc gcttggccca cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc
     8101 cacaagtgca ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct
     8161 ggcaccaggt cggcggcgga cgtctgcaac tgatgttttc tcgtaatcgc ctgaccactc
     8221 aggcaaaaca aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac
     8281 tgaccgccgt ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga
     8341 ttttcagcga atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa
     8401 aactggccgc gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt
     8461 accttgatgc aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg
     8521 agcgtaccac taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg
     8581 agatcccgga actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata
     8641 tcaataacct ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt
     8701 actggcgtca actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc
     8761 tgctgtctcg tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa
     8821 acgtcgccta ccgtatggag cgccagtacc gccagcttaa ccaggtggag atcctcggca
     8881 aaatcaacgg cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact
     8941 ggcatcagtt cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta
     9001 cccagattga accgcacgac tacatcgccg agctgtttga ttgcgttgca cgcttcaaca
     9061 ccattctgat cgactttgat cgtgacgtct ggggttatat cgcccttaac cacttcaaac
     9121 agaaaaccat tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg
     9181 acttcgagaa ctccgaaggg aatctgggcc tttccaacgc ggtattacag catctggcaa
     9241 gcaaactgcc ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc
     9301 tcggcgtggg tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca
     9361 aactggaagt gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg
     9421 ctgagccaat ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga
     9481 aagagctgac tcgtggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgacggtc
     9541 tggcgttgcc agaagaagag aaagcccgcc tgaaagcgat gacgccggca aactacattg
     9601 gtcgcgccat caccatggtt gatgagctga aataaactcg tatcagtgcc ggatggcgat
     9661 gctgtccggc ctgcttatta agattatccg ctttttattt tttcacttta cctcccctcc
     9721 ccgctggttt atttaatgtt tacccccata accacataat cgcgttacac tattttaata
     9781 attaagacag ggagaaataa aaatgcgcgt actggttgtt gaagacaatg cgttgttacg
     9841 tcaccacctt aaagttcaga ttcaggatgc tggtcatcag gtcgatgatg cagaagatgc
     9901 caaagaagcc gattattatc tcaatgaaca tttaccggat attgcgattg tcgatctcgg
     9961 attgccagac gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg atgtttcact
    10021 gccgattctg gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc
    10081 tggtgctgat gattacgtga ctaaaccatt tcatattgaa gaggtgatgg cgcgaatgca
    10141 ggcattaatg cggcgtaata gcggtctggc ttcacaggtc atttcgctac caccgtttca
    10201 ggttgatctc tctcgccgtg aattatctat taatgacgaa gtgatcaaac tgaccgcgtt
    10261 cgaatacacc attatggaaa cgttgatacg caataatggc aaagtggtga gcaaagattc
    10321 gttaatgctc caactctatc cggatgcgga gctgcgggaa agccatacca ttgatgtact
    10381 gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg caagaagtga ttactaccgt
    10441 tcgcggccag ggctatttgt tcgaattgcg ctgatgaaaa aattactgcg tctttttttc
    10501 ccgctctcgc tgcgggtacg ttttctgttg gcaacggctg cggtagtact ggtgctttcg
    10561 cttgcctacg gaatggtcgc gctgatcggt tatagcgtca gtttcgataa aactacgttt
    10621 cggctgttac gtggcgagag caatctgttc tatacccttg cgaagtggga aaacaataag
    10681 ttgcatgtcg agttacccga aaatatcgac aagcaaagcc ccaccatgac gctaatttat
    10741 gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat gaagatgatc
    10801 cagcctgact ggctgaaatc gaatggtttt catgaaattg aagcggatgt taacgatacc
    10861 agcctcttgc tgagtggaga tcattcgata cagcaacagt tgcaggaagt gcgggaagat
    10921 gatgacgacg cggagatgac ccactcggtg gcagtaaacg tctacccggc aacatcgcgg
    10981 atgccaaaat taaccattgt ggtggtggat accattccgg tggagctaaa aagttcctat
    11041 atggtctgga gctggtttat ctatgtgctc tcagccaatc tgctgttagt gatcccgctg
    11101 ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa agaagtccgc
    11161 gaactggaag aacataaccg cgaattgctc aatccagcca caacgcgaga actgaccagt
    11221 ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg aacgttacga caaataccgt
    11281 acgacgctca ccgacctgac ccatagtctg aaaacgccac tggcggtgct gcaaagtacg
    11341 ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg ctgagccggt aatgctggag
    11401 caaatcagcc gcatttcaca gcaaattggc tactacctgc atcgtgccag tatgcgcggc
    11461 gggacattgc tcagccgcga gctgcatccg gtcgccccac tgctggacaa tctcacctca
    11521 gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct ctctcgatat ttcgccagag
    11581 atcagctttg tcggtgagca gaacgatttt gtcgaggtga tgggcaacgt gctggataat
    11641 gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc
    11701 tatattgtgg tcgaggatga tggccccggt attccattaa gcaagcgaga ggtcattttc
    11761 gaccgtggtc aacgggttga tactttacgc cctgggcaag gtgtagggct ggcggtagcc
    11821 cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg gagagagcat gctgggcggt
    11881 gcgcggatgg aggtgatttt tggtcgccag cattctgcgc cgaaagatga ataaatatgt
    11941 ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac cagcctgtgg
    12001 atgcttaaca tggaatacca actcactctt aactggcccg attttcttga acgtcactgg
    12061 cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc gatctctcca
    12121 gacgagttgg cgggtctggc gatggaaagc gaagttgaca gtcgactggt cagtcaccag
    12181 gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct acgatcatct cggtgaaacc
    12241 aactggtcat tactggtaca ggcagtgaac cactggcatg agccgaccgc cgcgctgatg
    12301 cgaccgttcc gtgaactacc ggactggcgt attgatgatc tgatgatttc tttttctgta
    12361 cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat tcagggtacc
    12421 ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg cccacatccg
    12481 gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga gcctggtgat
    12541 attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa tgcgatgaac
    12601 tattccgtgg gctttcgcgc gccaaatacg cgggaactga ttagtggatt tgccgattat
    12661 gtgctgcaac gtgaactggg cggcaactac tacagcgatc ctgatgttcc acctcgcgct
    12721 catcctgcgg atgttctgcc tcaagagatg gataaactgc gtgagatgat gctcgaattg
    12781 atcaaccagc cggaacactt taagcaatgg tttggcgagt ttatatccca gtcacgtcat
    12841 gaactggata tcgcgccgcc agaaccgcct tatcagccag atgaaatcta cgatgcgctg
    12901 aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac
    12961 gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc
    13021 agcaacattg cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc
    13081 gcgatgctcg cggcgctggt caatagcggg tattggttct tcgaagggta agtttggatt
    13141 taagccggat gcggcatcac atgccgcatc ccttttcgct tacttccgtt gcgccgttaa
    13201 ctcggcaata cggacgatca cctgcaccgc tttttccata ccttccagag tcacaaactc
    13261 atgcttacca tgatagttgt aaccgccagt gaacaagttc gggcacggta atcccataaa
    13321 cgacaactgc gcgccgtcgg taccaccgcg gatcggtttc agttccggtt caatatcgca
    13381 gtcacgcatc gcctgctggg cgatatcgag aatatgcgga tgctcaacca ctttctctcg
    13441 catattgtag taactgtctt caatcaccag ttcaatgtag caatcaggat gtaacccttt
    13501 gcccactttt ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc
    13561 gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag
    13621 gtgatagaaa ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc
    13681 atgaatacgt gccgccagcg acagcgcatt taccatcact cctttcgccg tgcccggatg
    13741 aacattgtta ccgacaattt tgatattgac cgatgcggcg ttgaagtttt caaactccag
    13801 ttcgcctacg ccaccaccgt caacagtgta agcccagcgg gcatcgaagg catcaacatc
    13861 aaaatgtttc gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc
    13921 atgcggaatg tttttctgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc
    13981 tttgtcatcg gcacctaaca aggttttacc atcggtggta atcaacgtct gacccagtag
    14041 ctgatgcagc accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat
    14101 atcgccaccg cgatagtttt caacaatttg cggattaaca tttttgccgc tgcaatccgg
    14161 tgaggtatcc acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg
    14221 taacgtcgcc atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatttcttc
    14281 gagctgctct ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct
    14341 cacccctgct tttgattggg tatccagaga tacgtagttc aaaaatcgct caagtagttt
    14401 atccatgtag tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat
    14461 attgcgtcag gtcactttta cccctgcaag cgggaatatt tatcagcatt accttaatga
    14521 atataaagct aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga
    14581 attaccgccc tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc
    14641 atccgttgcg tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca
    14701 ccgctggtgc aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag
    14761 ctggatctga ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt
    14821 aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg
    14881 ctggataacg aggacatcac ccacgttccg gcggaaaacc gctatgtgaa cactgttttc
    14941 caaagctacg cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc
    15001 atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga tggaagccct gcggatggtg
    15061 caactggaaa ccttcgctca acgtaaaccg catcagctct ctggtggtca acagcaacgc
    15121 gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc
    15181 tcagcgctgg attacaaact gcgtaagcaa atgcagaacg agctgaaagc gttacagcgt
    15241 aagcttggta ttaccttcgt ctttgtcact cacgatcagg aagaagcgct caccatgtca
    15301 gacaggattg tggtgatgcg cgagggtcgt attgagcaag atggtacacc gcgcgaaatt
    15361 tacgaagagc cgaaaaacct gtttgttgcc ggcttcattg gcgaaatcaa tatgtttaac
    15421 gccactgtca tcgaacgtct cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa
    15481 tgtaatatct acgttaactt cgccgttgaa ccggggcaaa aactgcatgt tctgctgcgc
    15541 ccggaagact tacgtgttga agagattaac gacgacaacc acgctgaagg gctgattggt
    15601 tacgttcgtg agcgtaacta caaaggcatg acgctggagt cggttgttga actggaaaat
    15661 ggcaagatgg tgatggtcag cgaattcttc aatgaagacg accctgactt tgaccactct
    15721 ctcgaccaaa aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa
    15781 gaacacaagt aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt
    15841 tgtctttctg cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag
    15901 tttcgtcaaa atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt
    15961 tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct
    16021 gggctacccg tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct
    16081 gtttctgctg attgttccct tctggaccaa ctcactgatt cgcatctacg ggctgaaaat
    16141 tttcctcagc accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgacac
    16201 accaattcgt atcatgttca cgcccagtgc ggtgattatc ggtctggttt acattctgct
    16261 accgtttatg gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga
    16321 ggcggcgcgc gatctcggtg ccagcaagtt acagactttt atccgtatca ttattccact
    16381 gacgatgccg ggaattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt
    16441 ctatgtatcc gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt
    16501 ccagttcctc aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat
    16561 cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct cgtctgctga ataaaaaggt
    16621 ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac
    16681 ctgtatatcc caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc
    16741 aactggcagg gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta
    16801 caggcagcgc agcattcact aacaatggcg gtgttttcgg cgacatttgc tacgcttatc
    16861 ggttcactga cggcagttgc gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc
    16921 ggaatgctgt ttgtggtgat gatgtcacca gatatcgtga tggcgatttc tctgctggtg
    16981 ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc
    17041 ttctgcctgc cgtttgtggt ggtgacggtg tattcgcgcc tgaaaggttt tgacgtgcgg
    17101 atgctggaag cggcgaaaga tctcggtgcc agtgaattta ccattctgcg aaaaatcatt
    17161 ctgccactgg caatgccagc ggtggcggcg ggctgggtgt taagctttac cctgtcgatg
    17221 gacgatgtgg tagtttcttc gttcgtcacc ggaccaagtt atgaaattct gccgttaaaa
    17281 atttattcga tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta
    17341 ctggtgctgt cgctggtgat ggttattgcc agccagctta ttgctcgtga taaaacgaaa
    17401 ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc
    17461 tggcactggg catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact
    17521 ggaccgagta cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg
    17581 ctatctattc gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa acgtacaaag
    17641 acggtgccta tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagaag
    17701 ggatgatcca gaagatcgac aagtcgaagt taacgaactt cagcaatctc gatccagaca
    17761 tgctcaacaa gccgtttgac ccgaataacg actactccat tccgtatatc tggggtgcga
    17821 cggcgattgg cgttaacggt gatgcggtgg atcctaaatc tgtcaccagt tgggcagatc
    17881 tgtggaagcc agagtacaaa ggtagcctgc tgctgaccga cgatgcccgt gaagtgttcc
    17941 agatggcgct gcgcaaactg ggctactccg gtaacaccac cgatccgaaa gagattgaag
    18001 ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac tccgataacc
    18061 cggctaaccc gtacatggaa ggcgaagtta acctcggcat gatctggaac ggctctgctt
    18121 tcgttgcacg ccaggcgggt acgccaattg acgtggtgtg gccgaaagaa ggcggcattt
    18181 tctggatgga cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcgctgaaat
    18241 tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt tgccgaaact atcggttatc
    18301 caacgccaaa ccttgcggcg cgtaagctgt taagcccgga agtggcgaac gataaaacgc
    18361 tctacccgga tgctgaaacc attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca
    18421 gcagcattta tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaaacggg
    18481 cggtggtatt accgcccgtt ttttagttag caaggatgtg gctaatgagt caggacagta
    18541 aagttttatc ccgctctttc gtcggtattg tggtcccctt gtaggcctga taagcgtagc
    18601 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
    18661 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
    18721 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
    18781 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
    18841 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
    18901 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
    18961 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
    19021 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
    19081 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
    19141 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
    19201 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
    19261 cgcgaaaggt acgaatacct gattccgcag aaattcctgc ccctgtcagt acgagtactc
    19321 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
    19381 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
    19441 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
    19501 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
    19561 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
    19621 tgacgaccag gtcagagtca acgatggtca ggatatttcc cagacaaacc gctaataaat
    19681 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
    19741 tttcgggagc ctgcaacggt tgatgataat agtgttgcca gagccacgca aagccccgac
    19801 ctgatagata attttcgata caaccaattt gaccacagcc gcagcggcgt aacgggaaat
    19861 ccagccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
    19921 tgtagctttt accggtaatc ggtttaccgt tgaaaatcag cccgccgcca acgccggtgc
    19981 cgagaattaa ccccatcacc agtggatatt gcgtaaattc atcatcccag gcttctgaaa
    20041 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
    20101 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
    20161 ctgtttccgg cattcccgga ataccgatgc caacagaacc tttacagcca aaacgttggt
    20221 cagcttcggc taccagctcg catactgcat ctaaaaatgc gtcatagctg tcacgcggtg
    20281 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
    20341 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
    20401 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
    20461 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
    20521 aattccgatg gcaggaagtc aataaaatag atatcgctgg agaggaactg atgaccgatc
    20581 agcttttcaa tccgctcaat aatcggggta agttgcagtg aaacaactac gccgataatc
    20641 acaccacaca ggctaccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
    20701 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
    20761 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
    20821 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
    20881 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
    20941 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
    21001 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
    21061 aactgcaaaa taccggcaat gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
    21121 ggattcgagt tgggaatcat gatcgacacc caatcgccct gcttcacttt cagcgcatcc
    21181 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccaggcatcc
    21241 ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
    21301 acctgaattg cgcgcagatt ggtaccactt tctaccagtc cggtgaaatt gatatacggc
    21361 gcagcggcgg caatacccgg cactttttgc acgttatcca gtgcttcctg ccagttagtc
    21421 catggctgat ttaccgcctc aatttcaccg tgcggtacca cagcaagaat gcggttattc
    21481 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
    21541 gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg tcgccgtccg
    21601 cgactaaaac gcaggccaat caataacgat aaaggcatcg ccattactcc gcccccatca
    21661 ggctcagttc cgccgtcaga cgtccatcac gcatttccag ttggcggctc atacgtttcg
    21721 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
    21781 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggctcatccg
    21841 caagtaccag acgcggatta ttgaccagcg cacgggcaat cgccacacgc tggcgttcgc
    21901 cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca
    21961 tctccagcgc acggctgttg atttcagcgg gctttttctt gccaatcagc agtggcatag
    22021 ccacgttttc cagggcagta aaatccggca gcaggtggtg aaactgataa ataaagccca
    22081 gcttctggtt gcgcagttct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
    22141 taaagatcac atcgccggag gttggtgtat ccagcccgcc cagcaggtgc agcaaggtac
    22201 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
    22261 tgacattgtg cagtacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
    22321 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
    22381 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
    22441 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
    22501 atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc tccgaggatc
    22561 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
    22621 gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccattac catcagccct
    22681 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc
    22741 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
    22801 catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc
    22861 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
    22921 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa attgccaatc
    22981 acattgaaca ggcgctggct tggaatacgc cccatcggcg tgaactggct ggcagatggt
    23041 accatcacgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
    23101 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc
    23161 gtaagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
    23221 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
    23281 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
    23341 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
    23401 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
    23461 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    23521 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    23581 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    23641 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    23701 tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata acccgcagga
    23761 tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    23821 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    23881 tttatccgca tatgaccact tttcagcatc cgttatcgga agtcctgagt aaatgctgga
    23941 tctatttcaa tctttacctt gcaccctttc gtatgccggt ttttttcttt atatccggct
    24001 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    24061 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    24121 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    24181 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    24241 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    24301 tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    24361 tgaacagtgt gatccgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacaa
    24421 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    24481 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    24541 ttgtggtgat catgaagctg ttttatcagt acgagcaacg attcggtatg cgctccacca
    24601 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    24661 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    24721 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    24781 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt
    24841 cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc cccagccggg gcgattcaat
    24901 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    24961 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    25021 acactgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    25081 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    25141 gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg
    25201 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    25261 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    25321 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    25381 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    25441 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    25501 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    25561 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    25621 gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    25681 cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    25741 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    25801 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    25861 ctggaaacca gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc
    25921 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    25981 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    26041 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    26101 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    26161 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    26221 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    26281 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    26341 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    26401 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    26461 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    26521 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    26581 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    26641 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    26701 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    26761 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    26821 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    26881 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    26941 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    27001 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    27061 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    27121 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    27181 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg
    27241 aacgtggata ttctgacgct tactgcaacg ccgatcccac gcacgctgaa tatggcaatg
    27301 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    27361 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    27421 cgcggggggc aggtttatta tctctacaat gatgtggaaa acatccagaa agctgccgaa
    27481 cggctggcag aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag
    27541 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    27601 acaaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc
    27661 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcgcat
    27721 catcaggcat atgcatggtt gctgacaccg catccaaaag cgatgactac cgatgcacaa
    27781 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    27841 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    27901 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    27961 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    28021 atgccgtcgc tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat
    28081 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    28141 gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc tggatattgc ccgtctgcgc
    28201 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    28261 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    28321 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    28381 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    28441 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    28501 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    28561 aataacctta tatttattat tgatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    28621 taacgttttt tacgttcgcc gctgccgtgg cgctaccggc aaaagccaac acctagccgc
    28681 tgccgccagc gagcagtcgt ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg
    28741 gtggttctct ggaagccatc gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg
    28801 ctaaccccgg cgttgatcct tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc
    28861 aaaccctact tccagacgcg ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc
    28921 tctattacta cccgccgggt aaaaattcgg taaccgtgta tccaataggt attggtcagt
    28981 taggtggtga cacgctgaca ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc
    29041 caacctggac gccaacggca aacatccgcg cccgttataa agcacaggga attgagttgc
    29101 ctacggtagt gccggctgga ccggataacc caatgggcca tcatgcgatt cgtctggcgg
    29161 cctatggcgg cgtttatttg cttcacggta cgaacgccga tttcggcatt ggcatgcggg
    29221 taagttctgg ctgtattcgt ctgcgggatg acgatatcaa aacactcttt agccaggtca
    29281 ccccaggcac caaagtgaat atcatcaaca ctccgataaa agtctctgcc gaaccaaacg
    29341 gtgcgcgtct ggttgaagta catcagccgc tgtcagagaa gattgatgac gatccgcagc
    29401 tgctgccaat tacgctgaat agcgcaatgc aatcatttaa agatgcagca caaactgacg
    29461 ctgaagtgat gcaacatgtg atggatgtcc gttccgggat gccggtggat gtccgccgtc
    29521 atcaagtgag cccacaaact ctgtaaatcc tggataaaaa aaatccccgc gacacggcat
    29581 gtcgcgggga aaaaatcagc agtagcaggc attaatgagg gttaatgctt atttataaat
    29641 tactgctgtt ccatggaggg tattcggacc ggttacagaa gtaatacgga aagattttgc
    29701 gcccatctca tccgcttttt gcgccagctg ctcttccagc gatcccagat ttgtccccgc
    29761 gttagcactg atagtaccga ctttttgttg gccttctggc gttgactgaa cttcgacagc
    29821 cgcaaagttg gcaaatgaca tggagcttaa aatcgccgca gcgatgaggg tttttacgtt
    29881 tttcataata gtggccttat gcagatgaat gacgtcgcaa gaaattactt aacgatcgat
    29941 aactaaatga tagatgtgat ctggatcaca tacaagatat tttttataac aatcattaat
    30001 taatttaaaa atctttaaat ttcagttaaa tagaaagcaa ttatttttat gggatctgga
    30061 ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac caggttcaac
    30121 ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg
    30181 ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca cggttatgaa
    30241 gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac gctatacgcg
    30301 gaatttacca acaaagaggg gttatttcgt gccgttctcg accgctatat cgatcgtttt
    30361 gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct
    30421 gactattttg ctgccatcgc caactgcttt accagcaaag acaccccggc aggctgcttc
    30481 atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa tacgttgaaa
    30541 tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc
    30601 ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa ttgtattatt
    30661 caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc
    30721 ggaacgactt tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag
    30781 cctgagcgaa tgaggggcca aattgcagag tacagccttg ttatactttg ttattcaaaa
    30841 tcagttgccc gttactatct agcgggatct gcgtacccgg atcgcggtcc atgcggattt
    30901 tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt tctgacttgt
    30961 catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc
    31021 cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg gcgacatctt
    31081 taccacgacc accaccaaac tgatgcccta tcacgccgcc agcaacagcg ccgagcaccg
    31141 acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac
    31201 actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca acctgagcgt
    31261 attgcgggcc ccgttcaaac acgttcagac tggccactgc cgctacgccc agcgcagctg
    31321 cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct ccttctttgc
    31381 agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag atgaaaagcg
    31441 cgggaatggc tggaaatact taaggattcg gagaagtctg aaggaatgcc tgatgcgacg
    31501 cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga tgaggcgttt
    31561 atgccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg gataacgcgg
    31621 ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag cgcaatctga
    31681 tgcattcggt atagcgagat atatacaaag cgcgcaattc gtccttcaat catcattgag
    31741 ccgcgcgtca ggttacccat caggctaccg acagtggaga agttcgacag cgataccagc
    31801 gaaccatgat ctttatactg ataatttttc agcggtttac cgttcatctg cgccagaata
    31861 tttttcattg cgcaggtcgc catctggtgt gcagcctgag cacgcggcgg aacaaagccc
    31921 ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgggtg
    31981 gtttgcagcg tcggttccac caccagctgg ttgatacggt tggtttcaag accaccgata
    32041 tctttcagga agtctggcgc tttgatcccg gctgcccaca ccatcagatc agcctcaata
    32101 tattcaccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat ggtttgcgtc
    32161 agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat acgtggcggt
    32221 aatgcaggca aaatacgttc tcccgcttct accagcgtta cgttcagggc ttcgttggtc
    32281 aggcctttgt aaccgtagct gtgcagttgc ttgaccgcat tgtacaattc agcggagagt
    32341 tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttaccatt cgcgcccagg
    32401 ttggcggagt atttcaggaa cagattcagc atctcctggt ggaagcgacg cgcctggtgc
    32461 gggttatcga ggaaaatgca gttctctttg acacctggcg tattgaaatc gttagaggtg
    32521 ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac cagcagttca
    32581 cctttctcat cgcgcagttc tgcaatagtg attgttttcg cttcacgatc aatatcaatg
    32641 acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata gctcaacgca
    32701 tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagcggttt ccacagatgg
    32761 ctgtggttac gatcgaccag cgtaattttg gcttttttct tgcgtcccag cttatgcccc
    32821 agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat ttttttcaat
    32881 ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat ggttataaac
    32941 atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat tgagaataac
    33001 atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg ctgaaaagat
    33061 aggcgacttt accgggtgag atcaaaaaac agagtttatt gtttattatt tgagcaagtc
    33121 cggaagggtg atatagggtg acgcgtatat cgagggcgtt tggtcgtcct gataagatgc
    33181 gcagcatcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtaaacg ccttatccga
    33241 cttacagaat tcggagcgca tcaggtaccg gcaaggttac ctgatgcgac gagggcattt
    33301 acccgagggt tttgaacgcc ttaatgcgct gcaaatgcgg ggagatattc ttgaatttgt
    33361 gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca gatgtccgct
    33421 ggctgttaag cgcttcatca ttacgcgaca aaatcaccag gcaacgatcg cgattcttct
    33481 cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg cgatcaatct
    33541 tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg cggatatcgc
    33601 aaagaaaacc aatccgttcc gcccagtatc cgcccagacc aacgccgcaa atcagcggac
    33661 gctcgtcgac gttcagttgc aacattttgt ccacttcttt gagcagatgc tgcatatcat
    33721 gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata aactgcaatt
    33781 gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa tagataatca
    33841 tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca gcgttcatgt
    33901 aactgattca gacgggtgct gatcgccttc cagcgagccg agtccatcag ttcctgtcgc
    33961 gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg tgacagatta
    34021 tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc gcaacctgcg
    34081 tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc ttccatcgat
    34141 aaatcgtcag agaaaattac accgtcaaaa cacagttcct gacgcaaaac ggttttcagc
    34201 cagtaggaag aaccgctcgc cggacgcgga tcaacatcac tgtagatcac atgcgcaggc
    34261 ataatggcgt cgagtttatt ttcgcggatt aaggaactga agaccgacat atctttagcg
    34321 cgaatctccg cttgtggacg tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact
    34381 gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc cggcttcatg cataccatca
    34441 ataaaccggc tggcaattgc cagggctttt tgtggatcgg catgataaga acgctcgcca
    34501 atcgccgcgc tgatatgccc gacatccagc actggcgcaa agctgatatc aatatccata
    34561 gcgatcattt cgctggccat cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc
    34621 attcccaaca gcgcagcaaa ggattgtgct gccggtaagc gggtaaaacc ttcgcgaaaa
    34681 cgctgcacgc gtccaccttc ctgatctacc gccaccacca gatgattgcg cgatgctgcg
    34741 cggatctggc gcaccagttc acgtaactgg gcaggatcat gatagttacg cgtaaagaga
    34801 atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc cagttcgtaa
    34861 ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta ttaatagctg
    34921 ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccgatttgtc gccagcggta
    34981 ctcaaaccac cctgctttga gcatcagcag ccagggaaac catcgcctga cctgacgcca
    35041 taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg
    35101 ttcagtattt tccacccaca ccgccgccag ttccagcgcg atatcaccat ctccggcata
    35161 ctcccagtcg atgagtttta accctgacgc gctatgcact aaatttccgg cgtggacatc
    35221 catatgcaat ggacttaagc gtaaaggccg tggttcccgc gctttgcgca gacgttttaa
    35281 cattcgcagc caacccacag tccgccgcgc cggatcgctt tgctgccagt acagttccag
    35341 taagggcaac agcgttattc gccagccaaa acgtggttgt tgatgaagat aatacagcaa
    35401 gcctgccagt tcgttggtat ccggcaaata cgtttttacc gcgccgggca gatagtcgac
    35461 taccatccag tcacggagat ataaatgcgg cttcggtgca atgcttgcgg gtagttgtga
    35521 taaagcccga tactggcgca agaacgcgga ctgcggcgca tcaggatcgt gcggctgacg
    35581 cacaacaaaa cgctggccct gatgttcaat gagaaaacta ccgccactaa gcccactatt
    35641 aaacgtcgtg acgggatgaa actgcgggaa aaagcgcgac agcaattcgt cgcgcgtgat
    35701 gggattattg ctgcgaaacg gcacctttac ctgaccagat aatttcgccc gtctgcacca
    35761 gcatcagctg catttgtagg gtcggagcct taacgttgcc agaggcgctg gagtacagca
    35821 cgtaatgagc gccgacattg cgggcaatgc ctatggcttt actacgggta cctaaactgt
    35881 cctgcggcga caaacctaac tgttgcttag ccatcgacag ctgctgggcg gaaaccaggg
    35941 taaatttccc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct gcggcattca
    36001 gcgaaccgtt agtacggttg ttaacgctat caaccagcag gacgctaccc gcagtcaccc
    36061 cgtcagcccc aagcatctta ctgaccatcg gctgcattgc gccattccag tcataatggc
    36121 gaatatgcgg cgcaggcggt gccgtttgat cttcgtgctc aattgggcct ggctgctgcg
    36181 ggatcgtcgg caccgagggc actgtgggga caggctgttg tggctcggct ggttgttccg
    36241 gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac ccggcgagaa
    36301 acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag attcacccct
    36361 tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct gccgtacagc
    36421 gttaccgccg aatgtgcggg aatgctaacg ctgcgtggcc tttccagagg atgcatctcc
    36481 agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc ttgcctttcg
    36541 ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac gggcttttct
    36601 gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc atcattcacc
    36661 ggaatttccg gatgtgaacg acagcccacc agcagtaaca acgccagaga caccagccca
    36721 aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc agcggacggc
    36781 cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg ttggtattca
    36841 tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt gccgctacgg
    36901 tgatcatgcg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca gtcggaatga
    36961 ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg cgaaacgccg
    37021 ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata attttgctga
    37081 atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt gcgccatgct
    37141 acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct ttcaacctta
    37201 actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca ttattcccat
    37261 ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac aaatatgaat
    37321 gcgcatattt ctcatttgca tttacaaaca aaattattcg cacactaaat ataaccgctc
    37381 atcaccctcc tccggcatga gcctgacgac aacataaacc aagagatttc aaatgctttc
    37441 aacacaattt aacagggata atcaatatca agccatcacc aaaccgtcac tacttgccgg
    37501 ttgcatagca ctggcactat taccttctgc cgcttttgct gcaccagcca ctgaagaaac
    37561 ggtgattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt acagcgtaac
    37621 gtctacctct gcgggtacca aaatgcagat gactcaactt gatattcctc agtcggtcac
    37681 tattgttagc cagcagcgga tggaagatca gcagttacaa acgctgggcg aagtgatgga
    37741 aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt attattcccg
    37801 tggattccag atcgataact atatggttga tggtatcccc acctattttg aatcgcgctg
    37861 gaatctgggc gacgcacttt ctgatatggc actctttgaa cgcgtagaag tagtgcgtgg
    37921 cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata tggttcgaaa
    37981 acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta gctggaacaa
    38041 agaacggtat gtggcggatt tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg
    38101 aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaaca gtgaaaagac
    38161 cttcttctcg ggcattgtcg atgctgattt aggcgatctt acgacgcttt cagccggtta
    38221 cgaatatcag cgcattgatg ttaatagccc aacctggggc ggtttaccgc gctggaatac
    38281 tgatggcagc agcaacagtt acgatcgtgc acgcagtacc gcacctgact gggcgtacaa
    38341 cgataaagag atcaacaagg tctttatgac cctgaagcag cggtttgctg atacctggca
    38401 agcgacactg aatgccaccc actctgaagt cgaatttgac agcaaaatga tgtatgtcga
    38461 tgcctatgta aacaaagcgg atggtatgct ggttgggcca tacagtaatt atggacctgg
    38521 ctttgattat gtcggcggca ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga
    38581 tttgttcgct gacggtagtt atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg
    38641 cagttacagc aaacaaaaca atcgttactt cagttcatgg gccaacatct tcccggatga
    38701 aattggcagt ttctacaact ttaatggcaa tttcccacaa accgactggt caccacagag
    38761 cctggcacag gacgatacca cacatatgaa atcgttatat gctgctactc gagtcaccct
    38821 tgccgatccg ctgcatctga tcctcggcgc acgttatacc aactggcggg ttgatacgct
    38881 gacttacagc atggagaaaa accacaccac gccttacgct ggtctggtgt ttgacatcaa
    38941 tgacaactgg tcgacctacg ccagctatac ctctattttc cagccgcaaa atgatcgtga
    39001 cagttcaggc aaatatctgg ctccaatcac cggtaacaac tacgagctgg gtctgaaatc
    39061 ggactggatg aatagccgtc tgaccaccac gttagccatc ttccgtattg agcaggataa
    39121 tgtcgctcag tccaccggta cacctatccc cggcagcaac ggcgaaaccg cctataaagc
    39181 ggtggatggg acagtcagta aaggggtgga atttgaactc aacggcgcaa ttaccgacaa
    39241 ctggcagctg acatttggcg caacgcgcta tattgcagag gataacgaag gaaacgccgt
    39301 taatcctaat ctgccacgca ccacggttaa aatgttcacc agctatcggt tgcctgtcat
    39361 gccagagttg acagtcggcg gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt
    39421 gacaccgtat ggcaccttcc gcgccgagca aggtagctac gcgctggtgg atctcttcac
    39481 ccgctaccag gtgacgaaaa acttctcgtt acaggggaac gtcaataacc tgttcgacaa
    39541 aacctacgat accaacatgg aaggttctat cgtctacggc gcaccgcgta atttcagcat
    39601 taccggcacg tatcaattct gaataacacc tgtaaaaaag gcagccatct ggcggcctta
    39661 gtctccccaa cgtcttacgg attagtggtt acggatgtac tcatccatct cggttttcag
    39721 gttatcggat ttagtaccga aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc
    39781 tgcgcccagt ttcttcaggc cagcctgatc cactttagac acatcagcaa cgctgacgcg
    39841 cagacgggta atacatgcgt cgaggttagt aatgttttct ttaccaccaa atgcagcaac
    39901 cagagccggt gccatttcgc tggtacctgt tgcttttgca tcttcagtcg cgtcttcacg
    39961 acccggcgtt ttcagatcca gtgctttaat cagcacgcgg aagatggtgt agtaaacaat
    40021 cgcataaccg ataccgacga tcgggaacag ccacagtttg ctgctgttac cagacagaac
    40081 gataaagtcg atcagaccgt gcgagaacga cgtaccgtca cgcatcccca gaagaataca
    40141 gattgggaat gccaggcctg ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa
    40201 catgaaggag aactcgatcg gctcggtgat accggtcagg aacgaggtca gcgccgcgga
    40261 gatcataata ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc agatagcaat
    40321 tgcggcagct ggcagaccgt acattttgaa caggaagcca ccagacagtt tacccgcagt
    40381 cgggtcaccc gccatataac gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt
    40441 gtattcacca atctgcatct ggaaaggtac gttccagatg tggtgcagac caaacggcac
    40501 caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact accgggttct ggtaagcagc
    40561 ccactgagag aaggtctgga ttgcagaacc aatcggcggc caaatgaagg acagcacaac
    40621 gccagtaaag atggcagcca ggccagaaat gatcggcaca aagcgtttac cggcaaagaa
    40681 gccaagatac tcaggcagct taatacggta gaaacggtta aacatgtacg ctgcgatcgc
    40741 accggagata atccctccga gtacgccagt atccgccagg tgtttagagg cgatttcttc
    40801 agcaggtaaa tgcagtacca gtggcgcaac cacggccatg gttttaacca tgatgccata
    40861 ggcaacaact gcggccagcg cggatacgcc atcgttattg gtaaagccga gggcgacacc
    40921 gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg cctgcttctg ccataacatg
    40981 cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg acgcccagca gaatacctgc
    41041 gataggcagt acggataccg gcagcatcag cgatttaccg accttttgca ggttagcaaa
    41101 tgcattctta aacataattg agagtgctcc tgagtatggg tgcttttttt acgttctcac
    41161 gcgtggcaag gggggagagc ctcgccgtgt acagggcatc taagcgccct ttatttatta
    41221 cacagagtaa aataattcag tgccgatttg tttgacggct atcacatttc aacttcagat
    41281 cattggagaa gttgcacatt ttgacaaaac atatgtcaca atatttcaaa cgtggttatt
    41341 caccgcccaa ttttcggcgg tgaactttac tcgttttagc cattaattac gagctttaaa
    41401 aaaactcatt caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa
    41461 gttatcggtg gttacctgcg ccagttcttc aacggcaaca cctttcaaca cagccatgta
    41521 ttctgcaacg tcacgaacca tcgcaggttg attctctttt cctcgatgcg gtaccggcgc
    41581 aaggtaaggt gagtcagttt ccaccagtaa ccgatccagg gggacataac gcgcagcatc
    41641 tcgaagttgc tccgcattac ggaaggtcac aatgccggaa aaggagatgt aaaatccgag
    41701 atccagtaat ttacccgccg tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca
    41761 atccgtcact ttttcttcgc gcagaattgc cagcgtatcg gcgcgggcgt cacgggtatg
    41821 gacgataacc ggcttgttca gttcacgacc aatctggata tgatggatga aggactcttg
    41881 ctgacgaact ttagtttccg gtgtgtaata ataatccagc ccggtttcac ccagcgctac
    41941 aacaccctct tctgccgcca gacggcgtaa atcttctaca tcgtagggat cattctggtt
    42001 gagtggatgc acgccacaag aaaatacgac gttgtcacgt tcgcctacca gatcccgcat
    42061 atgtaaataa cccggtaatg ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc
    42121 ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat tcataatcca gaccatcgag
    42181 atggcagtgt gagtcgacta aaaacatgat gtctctctta aagatgagga accggtagca
    42241 caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg agaagctcgc
    42301 ggttgatgcc tgtgacagac attaactgtt cacgaatgtg gcaaacatcc cccagtatag
    42361 cctgcaggcg cgagggagaa agatggtttg ccagttcgac gacaaggccc ggcacatcaa
    42421 cattggtcac ctgcgcagca ccatgatggc gttttagcgc atccatcagc aacgttgcca
    42481 gccagtgtaa acgcgccgga gcttgttcat gattaagggc cgccagcagc gaataccagt
    42541 cgcccgatgg cacgctatat gccaatgcct gacacaatgt ttcacgagcc tgccagttat
    42601 ctccctgaaa caacgccagt gccgcgccag gcgaaccggc gcttaagcgc aatgcggcaa
    42661 gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg
    42721 gcggcggcgc aaggtaatgt aaccgacaac gactacgtaa tgttgccagt aaacgttcag
    42781 gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg ctcttcaagc gttttcagca
    42841 atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt tacccagaca actttcgcac
    42901 cacctaggcg tgcgtgctca ttcagctttt cggtgacctc acgtactgca tcaatgccca
    42961 gcgtattttt ccctttttcg ggagccaggg tgtagtaatc gggatgcgtg ccagcctgca
    43021 tcaactgaca tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt tggcagagta
    43081 aataacggct cagggcgtag attaaagcat catcgcccat gcccggtaac gcctgaatga
    43141 gtagcgcatg gtgacctctt ccggcctgat agctggctac cagtttttcg aaatcaggtc
    43201 gtaaccatgg ataccatctc atgcgtccaa ctccttcacc cagtgggtca cggtagtgcg
    43261 gattgcatcc atcacggcct ccagcggctg ggtggcatca atggtatgaa tacttttatc
    43321 ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta aagaaatcga aagactcttg
    43381 ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt aagccaactt ccggggtaac
    43441 atcgagatag agcgttaagt cggggcgaaa atccccgaga acagcatcac gcagtgttgc
    43501 cagcatatgt tggtcaatac cacgtcctcc gccctgatac gcctgagtgg agagatcgtg
    43561 gcgatcgcca atcacccagg tgccgttagc cagagctggt ttgatgaccg tttctaccag
    43621 ttgaacgcgc gcggcataaa acatcagaac ttcggcttta tcggtaatga cttcatcgcc
    43681 taccgatttg atatccagca ccaggcttct taacttttcg gcaagttgcg taccgccagg
    43741 ttcccgagtg aaaaccatgt cgcggatacc cagttgctca agcgtctcaa ccaccacatt
    43801 acgcgcggta gttttgcctg cgccttccag cccctcaatg acgatatact tactgcgcat
    43861 ttttttcctt aagcactttc agataatcct gcacagactt gttatggctg gcaagattgg
    43921 tattaaacgt gtgaccacct ttaccatcgg ccacaaaata gagatacggc gtttttgccg
    43981 gatgcgcagc agccttcagc gaatccgccc ccggcgtagc tatcgcaccc ggcggcaaac
    44041 cggtaatggt ataggtgtta tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc
    44101 cattataacg ctctcccatc ccgtaaatca cggttgggtc ggtctgcagg cgcataccaa
    44161 tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg ttcactggca acggcggttt
    44221 ctttttcgat aattgatgcc atcgtcacca gctgattttt atctttataa ggcagaccgt
    44281 ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga
    44341 gtaacgcgac atcggtggta ttggcggtat acatccaggt gtctggccag aaccaacctt
    44401 caatccactc cgggttttcc agttcaagtg cctgcgctac ggtggcgtac ttatcgtcac
    44461 tcagcgtgtg cttgatatac ggagcctcac gcaattgctt gaggtagtcg ctcagacgca
    44521 tcccttctac cagtcgcagc gggaactgtg cttctttacc gctttccagc aatttcagca
    44581 tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat
    44641 ccggttcgat acgcagcaac cattgaaaaa cccgtgggcg attgatgatc ttatcggcat
    44701 aaagctgttc accaagcgcc agacgtccgg tccctggctt cagggtaaat atcgtctctt
    44761 ctttgataag caatttgctg tcggcaagat ggcgaacctt ccagacgccc acaccagcgg
    44821 cgatacccag taccaccagc aataacaaga taatcaataa cacttttttc atgactaatt
    44881 cgggcgctca caaagtgggg ctaaatattc atataacgtt gctgacgaaa aggagacatc
    44941 gccacaggca cgtacgggca tcactggcat taacgcatta caaataacca tctcatctgc
    45001 ctgcaacgcc tcttccagag aggcttgcac ttcgacaagc tgataagagg attgtgccag
    45061 caaacggata cagaattgtc gcataatgcc gttaacacct gcctgatcca gacgcggcgt
    45121 ataaactaca ttgcccttcc gccagaacaa attagccgca cagcattccg taacccaccc
    45181 ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc tcaagatgag agcggatcaa
    45241 tacttgctca agccgattaa gatgtttaat acctgcaaga tgaggattgc gccccagccg
    45301 caccgggctt agcgccaacg tcatcccttc gttacgcaaa cggtcgtaat gtgcaggata
    45361 aaccgtaacg gagagaatcc gcgttgccgg tccactgttc aatgtgctgt accctcgccc
    45421 gccactaccg cgactgatca cgactttcag cacaccattt tgctgttctg ctgccagcgt
    45481 tttcatctct tgttcaagct gaggccagaa gtcacagaaa atcatcaacc gctgacaagc
    45541 atcctgtagt cgctggatat gcgccgataa caaactgact ttaccgtcga taactctggc
    45601 ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg cttactgcca gcgattcctg
    45661 cttatgaccg ttaattaaga acataccggc tccttatcag agaattgtta gtgtggcagc
    45721 atgttcacta cggaacaagt cggaataaaa gctaagaaaa aaggcccgca agcggacctt
    45781 ttatatgggt gggaaatgac aacttagatc tttttaaaga tcaaagaacc attagtgcca
    45841 ccgaagccga aggagttaca cagcgtgtat tccattccgc tgacctgacg tgcttcatgc
    45901 ggtacgaaat ccagatcgca accttcatcc gggttatcca ggttgatggt tggcggaaca
    45961 gcctgatcgc gcagcgccag gatggagtag atagattcta ctgcacccgc cgcacctaac
    46021 aggtgaccgg tcatagattt cgtggagctt accaacacac ggcttgcagc ttcaccgaag
    46081 atggttttca ccgcctgcgc ttcagcttta tcgccagccg gcgtagaagt accgtgcgcg
    46141 ttaacgtagc caatctgact cgcttcaatg cctgcatcac gcagagcatt tgccatcgcc
    46201 agagctgcgc ctgcgccatt ttctggcggt gacgtcatat gataagcatc gctgctcata
    46261 ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct
    46321 tcaagtacca gcataccggc accatcgccc agtacgaagc catcacgctc tttatcccac
    46381 gggcggctcg ccgcttgcgg gttatcattg cgggtagata atgcacgtgc cgcgccaaaa
    46441 ccaccaacac ccagcggcgt actggctttc tctgcgccac ctgcaaccat cacgtcagca
    46501 tcgccatacg cgataatacg cgcagcatgg ccaatgttgt gcacgccgga agtacaggcg
    46561 gtcgcgatag agatgcttgg gccacgcagg ccatacatga tagtcagatg acctgccacc
    46621 atgttcacaa tcgttgacgg aacgaagaat gggctgatct tacgtggacc accgttcatc
    46681 agagatgtgt ggttttcttc gatcagtccg aggccgccaa tcccggagcc aattgcggca
    46741 ccaatgcggg ttgcgttctc ttccgttata tcaaggccag aatcctgcat ggcctgaacg
    46801 ccagcgacaa ttccatattg aatgaaggca tccatcttgc gctgttcttt gcgcgagata
    46861 atgtcctcac agttaaaatc ctttactaag ccagcaaatt tcgttgcata ggcgctagta
    46921 tcgaaatggt cgattaggct gatgccactc tgaccggcaa gcagagcttt ccaggtagac
    46981 tctacggtat tgccgacagg agacaacatg cccagtccgg tcacaactac acgacgctta
    47041 gacacgtttg tcctccaggg agggaaaaaa tgattctagt gggacaaaaa gataaaactc
    47101 aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt gatgtaatca
    47161 atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc agtatcaaac
    47221 tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc caggtcttca
    47281 acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc gccgataatt
    47341 ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa actcgctttc
    47401 gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc cggcaggtct
    47461 taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc attttgcact
    47521 aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc aaatcgcggt
    47581 cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta agctgcttcg
    47641 tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag gcgacccgca
    47701 ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt
    47761 tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc
    47821 agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc ctgaccgcca
    47881 tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg ctttttcatc
    47941 atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttggt ttcgataata
    48001 tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat accggcatta
    48061 ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac agattcgata
    48121 gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa ataatcgctg
    48181 atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc acgggctgcg
    48241 agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag tgcgattttt
    48301 ccttcaaaat tcatgatttt cctcttttaa agctcaagcg ccgctgccat cgctgaaggt
    48361 tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc agtaagcacc
    48421 ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta ctcaacagac
    48481 ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat ggcatcacca
    48541 ttggtttcgc atttcacatc aacgttattc acaacagaaa ctgttggtgc gttaaaggtg
    48601 attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc acagtgagac
    48661 ggtacgctca ctggtaacgg cagagcacgt tttgcgcccg cagctttaca ggcagcgcca
    48721 gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga gttaaagttt
    48781 accggagaaa cgacctgacc tgctgcagct tcttcacacg ctttcgcaat agacgcatca
    48841 tccagaccga tgattgccgc catagcgccc gtaccttccg gtacggcttc ttgcatgagc
    48901 ttgccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacacc agcgcaaacc
    48961 agcgcggaat attcccccag gctgtgaccg gccatcattg ccggtgcttt accgccctgc
    49021 tgctgccata cgcggtacag cgcaacagag gcagtcaaca gcgctggctg ggtttgccag
    49081 gttttattca gttcttcagc tggcccctgc tgggtcagcg cccacaggtc gtagcccagc
    49141 gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc catatcagcc
    49201 agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg cgtcatgttt
    49261 taatccttat cctagaaacg aaccagcgcg gagccccagg tgaatccacc gccaaaggct
    49321 tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc atccagcgcg
    49381 cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac gacattgtcc
    49441 atagacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc ctgatgcgga
    49501 accagccagt ccagttgaga acggtcaaga ttatttgccg ccagcgtctc atcaacgatg
    49561 tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt cagatgaatt
    49621 gaattctctg gattcacacg gtcagcatta ggcagcgtca gcaactcacc atagctaccg
    49681 tcggcatgca gatgggtgga gatgattccc ggctcttcag aggcagccag caccgcagcg
    49741 cccgcgccat cgccaaaaat aataatagtc ccacgatcgg ttggatcgca ggtgcgcgcc
    49801 agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac gtattgatcg
    49861 gctacgctta atgcataggt gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa
    49921 cctttaatgc ccagcatgct ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca
    49981 gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc catctcaatt
    50041 gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg
    50101 cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac cattttttcc
    50161 aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat aatcttcgta
    50221 tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg cgagttcgct
    50281 ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct caaaaccagc
    50341 tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct gcaccgcctg
    50401 cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat gactttttat
    50461 caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt tgaggtgact
    50521 gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc accacgaccg
    50581 ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa caccttccat
    50641 cgtctttaat gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt
    50701 taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg ttttaagcac
    50761 cgctgaggca tcccgaatac tgtcgagacc ctttacttct tcttcaccaa tattgagcaa
    50821 cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc ccataatggc
    50881 aaattgcacc aacattgtac tgtcacaatc gacgttggcc cctaagtcaa ggaccaccgt
    50941 tttgcccttt tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa tcccctccag
    51001 gggcttgagt aataattttg ccagccccat cagtgccccg gtattaccgg cactgacaca
    51061 ggcttgcgct cgaccttctt tcaccagctc cagggccacg cgcattgaac tcccacgact
    51121 ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg caggaataat
    51181 ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga tggcgtcggg
    51241 attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca atgctgcagg
    51301 cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg ttagacgtgt
    51361 caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa atcctcacta
    51421 agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg gtagtaaccg
    51481 tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac agacaggctg
    51541 gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg ggttggttta
    51601 ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga tccaacgggg
    51661 agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg cttttgcact
    51721 tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg ttcagaatca
    51781 tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc aagcagatcg
    51841 atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt
    51901 tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt aaacggcttc
    51961 ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa cactgcgaga
    52021 cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac actgactacg
    52081 gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc aaggcgtttt
    52141 tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat agggcgcgca
    52201 tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg ccttttgcca
    52261 ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat aacgaacatc
    52321 aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta tatctgatgg
    52381 aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc gctcttctgg
    52441 aaaaactgca aatctctttt gaatgtgcag caccagaggt cgacgaaacc ccacgcagcg
    52501 acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt
    52561 cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt gatggcgaaa
    52621 tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa gccagcggca
    52681 atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg catctacaaa
    52741 cagaagtgga gccttttgac gtccatttcc gtcacctgag cgaggcggag attgataatt
    52801 acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa ggatttggca
    52861 ttacgctgtt tgagcgttta gagggacgtg accctaacac gctggttggt ttaccgctaa
    52921 tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga tagttgtttg
    52981 cgctggatgc ggttacgtcg catccggcaa tcggtgcagg cggcctgatg cggcgtaaac
    53041 gccttatcag gccaaccgcc cattatcagg cttatatgct tgtattagcg cgcgttacgc
    53101 agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg catcacctca
    53161 ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg atttaatccc
    53221 gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca
    53281 atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgtcc ggttaccggg
    53341 ctacaacgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg tgtttccgac
    53401 ggtttgcctt cctgactgac acgcacgata cgttcgccgc tttgcagaat atttttcaat
    53461 aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag cgccaggtaa
    53521 tctttttgca ttcctttttc acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt
    53581 ttcgctatca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac
    53641 cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa accactgccg
    53701 ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatgatcatc ttcatataag
    53761 atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc ttcttcccgc
    53821 tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt atattcaggc
    53881 ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta aatcatactt
    53941 tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca
    54001 gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggtggg cgattctaat
    54061 tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt gcaccttatt
    54121 acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca agaagtgaaa
    54181 aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca aggcttgcag
    54241 tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt tacggaataa
    54301 cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg atctttcagg
    54361 cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc tggagagtaa
    54421 tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg ggcaaccgac
    54481 actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact agacacgagg
    54541 ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa
    54601 taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg aagagttgcg
    54661 cgttgccctt gtagatgggc agcgtcttta tgacctggat atcgaaagtc cagggcacga
    54721 gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga gtctggaagc
    54781 tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag aaattgcccg
    54841 cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag atgtgttgcg
    54901 tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca aaggcgcggc
    54961 attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccaa ataacccgcg
    55021 cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt accgaattaa aagaagcact
    55081 ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg gcgtaggcaa
    55141 atctgctgag gcgctgcaat gggatttaag cttccgcctg aaacactggg aagccatcaa
    55201 aaaagccgct gaaagccgcc cggctccgtt cctgattcat caggagagca acgtaatcgt
    55261 tcgcgcattc cgcgattact tacgtcagga catcggcgaa atccttatcg ataacccgaa
    55321 agtgctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt tcagcagcaa
    55381 aatcaaactg tataccggcg agatcccgct gttcagccac taccagattg agtcgcagat
    55441 cgagtccgcc ttccagcgtg aagtccgtct gccgtccggt ggttcgattg ttatcgacag
    55501 caccgaagcg ttaacggcca tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat
    55561 cgaagaaacc gcgtttaaca ccaacctcga agctgccgat gagattgctc gtcagctgcg
    55621 cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc ctgtacgcca
    55681 ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca
    55741 aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgccagcgcc tgagcccatc
    55801 actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta ccgtgcgtga
    55861 caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa gaagaagcgc tgaaagagaa
    55921 cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttatctgc tgaatgaaaa
    55981 acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt gttcgctgcg tgattgtgcc
    56041 aaacgatcag atggaaaccc cgcactacca cgtgttgcgc gtgcgcaaag gggaagaaac
    56101 gccgacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga
    56161 agaagagttc gctgaacgta agcgtcctga acaacctgca ctggcaacct ttgccatgcc
    56221 ggatgtgccg ccagcgccaa ccccagctga acctgccgcg actgtcgtag ccccagcacc
    56281 gaaagctgca acggcaacac cagcagctcc tgcacaacct gggctgttaa gccgcttctt
    56341 cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc aaaccgaccg agcaaccaac
    56401 accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaaacctc gtcagagcaa
    56461 ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa
    56521 tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg agacgcgtga
    56581 gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc agcaagcgcc
    56641 gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac aagaagcgaa
    56701 ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc
    56761 ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt
    56821 agccgaagaa gcggtagtcg caccggtggt tgaagaaact gccgctgccg aaccaattgt
    56881 tcaggaagct ccagctccac gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca
    56941 aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg gtggcatgcc
    57001 gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta
    57061 tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg accgtagcgt gcgcgtctcc
    57121 ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc aagatgtaca
    57181 agttgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga ctggggtccc
    57241 tgtcgccgcc gctgtcgaac ctgttgttag cgcaccggtt gttgaagaag tggctgaagt
    57301 tgtagaagcc cccgttcaga ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc
    57361 tgaagtgatt gccgccgcag taactgaaca gccgcaggtg attaccgagt ctgatgttgc
    57421 cgtagcccag gaagttgcag aacacgcaga accggtggtt gaaccgcagg aagagacggc
    57481 agatattgaa gaagttgccg aaactgctga ggttgtggtt gctgaacctg aagttgttgc
    57541 tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa gttgaaacgg tagctgcggt
    57601 tgaacctgag gtcaccgttg agcataacca cgctacggtg ccgatgacgc gcgctccggc
    57661 accggaatat gttccggatg caccgcgtca cagtgactgg cagcgcccta cttttgcctt
    57721 cgaaggtaaa ggtgccgcag gtggtcatac ggcaacacat catgcctctg ccgctcctgc
    57781 gcgtccgcaa cctgttgagt aataattagc tcaagtaatc aagccctggt aactgccagg
    57841 gcttttttat ttcatctttg aatcaacata ttaaaattca tcgaatggca tccttgctaa
    57901 ccaacaatgc aaaataggca aaaaaatgcc cgccccggcg atcccagagc aggcagacaa
    57961 aacataccca gtttcatgat atgttttaaa gcgaaattat ttgttgagct ggaagagcga
    58021 caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat
    58081 gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg
    58141 ccctaaagcg cgatcgctac ctaatgaatc cagcgactcc agttcgttca gctgcgtgcc
    58201 taattccgcg cgaacggtca gcacattgtt cagcgagttt ttcagtccgc ggttggtttt
    58261 atctaacgcc gcagcggcgg tttctttatc cgcttcgcta tccgcgaccg gcgttttcag
    58321 ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg
    58381 ttccgctacc gcgttactgg taatactgtc gaaaattttg tcgcccgtat gtcctatcac
    58441 catcgaacgc gaagcatcga cctgttgctt aatactttct gttccgccga cataatcacc
    58501 gttagcctca ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt agcgcccatt
    58561 accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa tatccgtagc
    58621 cagcgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa ttttttcctg
    58681 agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg acactttttg
    58741 agtggcgaaa gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac
    58801 aaccgcttgt gatgcagcaa tgggatcatc tgaagggtta acgactcgct tacccgtcga
    58861 catctgttcg ccgtacttca tccattctgc ctgagaattg gtgataccac gcatgttttg
    58921 ctggtacatc atctgggtac tgaaacgcat tttatctccc cttagcgaat gttaatcagc
    58981 gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga
    59041 aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga ctgctgctga
    59101 ttggaaagct gcgtcaccac attaccttgc gtggtgctac tggttttcaa cgtcgcggtt
    59161 ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc
    59221 accgttttac tattgctttg cagatccagc agggcctgac cgttgcggtt atcgctatca
    59281 cccgcatctt tttcgctcgc catcgctatt ttcgcttcgt cggtgattaa tacatccatg
    59341 ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc aggcgttcct
    59401 gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatccgg cgtcaccgta
    59461 aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg
    59521 taatctgtcg ccagtaccac ggaggcatca gttaccgtgg caccgatcgc aacgtcacct
    59581 ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag taaagaaatc ttcaccagca
    59641 tcgccgttag catcaaatcc ggctttgtgt tgggtgttga aagcctcggc aaatgccagc
    59701 gccagttgtc caagcgtatt acgcgtctgg tccagctcct gagaacggaa tgtcagaatg
    59761 ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca
    59821 tcaacataag cgacagtcgt acgagaaggg tcagcgctgg aaggaactgc cgccagttgc
    59881 cgcgccgtac ttccctgaac cagtgagtaa ccattggcca ccgtgatgtt ataagtgccg
    59941 ccatcctgaa cgctgacttc tacaccaaca atctggttta attcgctcac cagttgatcg
    60001 cgttgatcca gcagattgtt aggtgacgcc cctgccccca cgcctgtcag gcgcgagatt
    60061 tgatcgttca ggctggcaat ttgtttagcg taattgttga tctgatcaac gctggcacct
    60121 atcgcgatat tgacctgttt gtcctggtcg cgcagatatt gatcggtggt tttaaactga
    60181 ttcaccaacc cttctgattt cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta
    60241 ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta
    60301 ctggtggaga gcatattgtc gattttcgac atctgctcat agcgggcggt caggccgcta
    60361 ctttgcgtct gcgcagcacg taactggttg gtaataaacg catcatactc acgctgcaca
    60421 ccagaaacgt agacgccatt gccaacccag ccgccagcgc ccaacgtgct attggcctgc
    60481 gccataatag tggtttggcg ggtatatccg gcaacgttat agctggagat attattactt
    60541 gccgtattta acgccgcctg ggccgcgttc agtccgctca tggcgttatt gatcaagctg
    60601 gacatggagg ttccttataa gccttcaatt acagagtatt atcggcagcg acccgccgga
    60661 cttgagttat tcagaacaga ttatcaatgt tcatgctgta ggttttgctc accttgtcgc
    60721 ttatcgattt catctgctgg atcatgttgg tgagtttacg ggcatagtga ggatcggtag
    60781 cataccccgc gtcctgtagg gcctgcgccc cctgctccgc actcgcggct gtcgtcacgg
    60841 cggcgtagcg cgggttacgt gttaacagcc caacgtaatc cgacaatgct tccagatacg
    60901 agctgtagac ccgaaatttc gcttttactt tcttcgcttc gccattttca tattcagtcg
    60961 tggtgatttc agtaactgga cctttccagt tgccagaggc tttgacgcca aacaggttat
    61021 agctcggctc gccgttttcg cggcggattt gccgttgccc ccaaccagac tccagcgccg
    61081 cctgagcgag gatcaaatga tgtggcacac cgctttgctg gcttgccagt tgggcgggca
    61141 gcgagagttg cgcgaggaat gctttactgt cacccggcag cgaatcatcg tagttacgtg
    61201 gcacggcctt ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc acagtttcga
    61261 gcgggaattt catcggtgct gctggcgtgg actcctctgg caatggttgt tctggcgtca
    61321 tctgtttaac catcatctct gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa
    61381 tctgctggtc atacatactg gtatacaggc gagtgtgctc gctgctgaac aggccatctt
    61441 ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac gaacatccct tccacctgac
    61501 gggccaccgg acggatattt gccgccggat cttcgcccgc tttcgccttt agttcgttga
    61561 gtgattgcgc atcccaggcc gcacttgcca gtagtttgct gtcgctgatc atcagatgat
    61621 ttccagtttt gcccgcagac atcccgcact ttgcattgat tgcagtatgg acatcagatc
    61681 catcggcgta gcgcccagcg cattgagcgc gcgcaccacg ttattgaggc tggcgctgga
    61741 acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac
    61801 cacagtctgt ccaccaccaa acggtgtatc tggctggctg acattggcct gacgattgac
    61861 tgttactgag agatttccct gcgctaccgc gcagctgtcg agggtcactt cgcgattcat
    61921 caccaccgaa ccggtgcgcg agttaatcac tactttagcg tcctgcgggg tgacattaac
    61981 ctgcatattc tggatatctg caaggaaacg gacctgggaa ctgttgccac ttggtacgcg
    62041 cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca cgcggttgat
    62101 ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca aattaagggt
    62161 attcccgacg ccaaactggc tgggcaattc acgttcaata accgcaccat tggtgatccg
    62221 tccaccgttc agttggttca cctgaacact gctaccgcca gcggaggctc ctgcgccgcc
    62281 aaccagaata ttgccctgcg ccagcgcata cacctgactg tcaacgccct taagcggcgt
    62341 catcaacaac gtacctccac gcaagctttt ggcatttccc atggaagaaa ccaccacatc
    62401 gatggtttgc ccctgacgtc caaacggagg aagtgactct gtcaccatta ccgcagcgac
    62461 gttttttagc tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt
    62521 attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc catccagccc
    62581 caccaccaga ccatagccaa tcagtgagtt ttgccttacc ccctgaacac tggtgagatc
    62641 gcgaatacgc tcagcctgag ccgccgtcgt aaccagtaga agaattaatg cagagagaaa
    62701 tttaatcacc acagcctcac ttacattggc gacaggttaa ggaagaaacg ctgcaaccag
    62761 cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc
    62821 acctgagtag acggtacggt attgctgccg ctgatagtgc gtggattaac cacgccagag
    62881 aagcgaataa attcggtacc ctgattaatg gcaatctgtt tttcacccac cacatgcagg
    62941 ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg
    63001 gcattggccc cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac atcggcacga
    63061 gcgttaccaa acagcccctg caaatagcgc ggcacagtat caaagccaaa attagtttta
    63121 ccgtcacggc tggcattcgc agaggagctt ttgctggcgc tgacgttctc ctgcaacacg
    63181 atggtcagcg tatcgccaat attgcgtggt cgacgatctt caaacagcgg ttgatagcca
    63241 tagttaatcg gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt cggaccggga
    63301 accggttgtg cactggtcgc cccctgcacc agcggcgtgg agggtatcca ggcgcagccg
    63361 gttagtgaaa gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt tttttgcatg
    63421 gctatcatct tcaaaaatca gtaaaccggt gaacctgcca ccggttaagc cttagagttg
    63481 cgtcagtttt tgcagcatct gatcggtggt ggacaccgct ttactgttga tttcgtaagc
    63541 gcgttgcacc tgaatcatat tgaccagttc ttccgccacg ttgacgttag acgtttcaac
    63601 atacccttga tacagcagtc ccgcgccgtt caggcccggc gtgctttcgt tcggtacacc
    63661 agaggattgc gtttcggtgt agaggttttc gccaatgctc tccagcccgg tgtcattcat
    63721 aaaggtggtg agattgagct gcccaacctg aaccggagct gcctggcctt gttgggttac
    63781 gctgaccacg ccatcacgac cgatggtgat acttaacgca ttcgccggaa tggtgatcgc
    63841 tggctgcacc tgaaaaccac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg
    63901 gtataggctg atgaaccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg
    63961 acatctttgc tgttgttggt ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg
    64021 accgggcgta cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac
    64081 tgtgcccccg gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc
    64141 ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc
    64201 tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg
    64261 gattagtgac aaagtgcata tgtcttcttg ccggatgcgg cgtaaacgcc ttatccggcc
    64321 tacaagtgct cgcaaaatcg ataaattgcg attgccccca tagacccgat aaacttgcgc
    64381 atcgggcaac ttatacttgt catgtatcct ttcaattaac tcatcgacag cagttggttg
    64441 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    64501 gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc
    64561 cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt ggcctgggtt
    64621 tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc
    64681 accagtttca gacgccctac tggcgcaact gtatttgccg gatcgcccgg attgagcgcc
    64741 gagattgtgc catcggcagc aatagtgatt tccgctcctt ccggcacagc aattggcccc
    64801 gcctcgccta tcacctgatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg
    64861 ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccagccatcc
    64921 tgctgcaacg cgacgtccag cgggcgcgag gtgtaatcca ttttgccggg cgtcatatct
    64981 gcgcccggcg ttgacgccgt gaccaacgtg cgcgtgggca gagaaagccc ttccactggc
    65041 accgcgcgta aagcattcaa ctgcgcgcga aaaccgggcg ttgaggcatt ggccagatta
    65101 ctggcggtta ccgcctgttg attcagtgtc tggctggctg ctcccatcgc ggtataaatt
    65161 gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg tgttgaggat
    65221 ctggtcctga gttttgatgg tctgggcgtt agactgatag ttacgctggg caacgatcat
    65281 attgaccagt tctttactga gatcgacatt ggacgcttcc aacgcaccgt tggtcagggt
    65341 gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga
    65401 ccagacgttg tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat
    65461 ctgccccagc agttgtgttt gttcgttgga atagttgccg acaaccgtac cgtcatcatt
    65521 gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatatt
    65581 gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg tggcgggatc
    65641 tgcgccgtta attgcgccag tggtaatatt ctctgttgga tttgaggtca gaacgccatt
    65701 ggcattaaac accagcttca ttgcaggctc ggctgtacct gttggatcac tgctatcctg
    65761 ggtgtagacc tgccagttat tatccccggt cttcacaaag tagacgctca tgtcatgagc
    65821 attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct
    65881 ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga
    65941 cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt
    66001 cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc
    66061 ttgcatattc accaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa
    66121 caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag
    66181 acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt
    66241 tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt
    66301 aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc
    66361 agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc
    66421 ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt
    66481 attaccgctg ttgccgcgga gcacaccctg caccagagca aactgcagcg gctgggcaac
    66541 cagttgtgta ccaccgttac tggcgctaat cgctacattg taagaaccgt tcggcgcagt
    66601 gctgccatca gtcaacgtac cgtcccaggt gaaactgtgg actccggcgg tcagttcacc
    66661 aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc
    66721 tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact
    66781 gccggttccc gcaagaacag tggtgccggg gatcatcacg ccgtgaccga tcaggttact
    66841 ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag
    66901 tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc
    66961 cattggattg gtcgggtcct ggtttttcag ctgcgccacc aacaaagtca gaaaactgct
    67021 ttgtaagtct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt
    67081 atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc
    67141 gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag
    67201 ctgcgtgacg ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt
    67261 acgtagccct ttgcatccgc cagcggatta cccggttcat aaaccagttt gtccggggcc
    67321 tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta
    67381 acctggaata ccacctgttt tgcccgatat ggctgtccat cgggaccggt cacgctatca
    67441 gcattcgcca gattactggc cgccacgttc aagcgctggg actgggcagt taacgccgac
    67501 ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc
    67561 atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta
    67621 tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg
    67681 tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc cggaatgtgt
    67741 tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg ttgcatgact
    67801 tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg
    67861 gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga
    67921 aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc
    67981 catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct
    68041 atttatgccg ttgatgacca ttgcggacag gtacaattca cgttgtagaa atggctgggg
    68101 gcgaaaatgc tggcaataaa acgtagcgtg gcgatcatct cgatactatt cactccgtta
    68161 agtgcggcga gtaatctcac atcgcaattg caaacctttt ttagcgccca gctcgcgggg
    68221 gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc gccgtgcgag
    68281 cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca
    68341 cgctgcggta acgacaaacg atatttacag gttaatgtac aggccacagg aaattatgtg
    68401 gttgccgcga tgcccattgt gcggggagga aagctggaag ctggcaatgt caaactaaaa
    68461 cgcggacggc aggataccct gccaccgcgt acggtgctgg atatcaatca acttgttgat
    68521 gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag
    68581 gcatggcggg taaaagcggg acaacgcgtc aacgtgatcg ccagcggtga cgggtttagc
    68641 gccaacgcag aaggtcaggc gctgaacaat gcagccgtca cacagaatgc gcgggtgcgc
    68701 atggtatcgg gactggtggt cagcggcgtt gttgatgcag atgggaatat tcttataaac
    68761 cagtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc
    68821 gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa gcccgtaagc
    68881 accgttcaac cacgcgaaac cactgacgcg tcggtaacga acacccgggc ggcaaaaaca
    68941 acagcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc
    69001 ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt
    69061 gaactaaaaa tggacaccgg caaaattgcc gatgcgctgc tcaacgaagc gcagcaagac
    69121 ttgcagagta actgatcgta tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt
    69181 gcttaacgat ctcaaaacgg taatggatca agaacagcaa catctctcta tggggcagat
    69241 caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg cgacgctgga
    69301 ttacctcgaa cagttacgca ggaaagaacc caatacagca aatagcgttg atattagtca
    69361 acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc aacataacgg
    69421 ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca
    69481 tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaacccatc gcggcggtaa
    69541 aaagatttcg atctgaatat tatctccggc ctgcactgca ggccggaatg cattgttaca
    69601 ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg
    69661 cggcaatccc cgccagcacg accgccatta aacgcagtaa gcgccagggc atggtaccca
    69721 atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg
    69781 ccaccaccag acgcaacaga aacgccatcc agccgggttg cggagtaaag attttctgct
    69841 tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg
    69901 acagcccagc atgtttcaac ggaccaataa acgccaggtt catcaattgc gttaaaatca
    69961 gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag
    70021 ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct
    70081 gggtcatcag cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa acggttaacg
    70141 gacccgaaag aatgcccaac gcaaccgcac tcggcagcgc caacaggaaa caaagacgca
    70201 acccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca
    70261 gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accggaggga aactccatta
    70321 agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag gcaaaaatgg
    70381 tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggt cccatctgtt
    70441 tcaccacgcg cattgctccg gcatcgtgga agttaatgcg cggcaggacc agcatgccga
    70501 tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc gtaacagccc
    70561 acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca
    70621 tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga gaagcggttc cacgtattca
    70681 gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag gtaatcttta
    70741 gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca
    70801 tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc gcaagtgtca
    70861 gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat
    70921 attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgta
    70981 acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga
    71041 ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc
    71101 tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc cacgggcaaa
    71161 acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg gtgaattgct
    71221 accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata
    71281 cgttgcgcca gcactgcctg ttcgccggcg gtttgcagaa ctgtctgatt ttgcacacat
    71341 tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagagtgct ctgccaacca
    71401 ggaatcggtt tatgcactac gccctgcccg cgctcctcac gccattcgcg catatccgta
    71461 atgtcgatga gcgcatcgtc agtcacggcc tgcacggttt cacgctgact tccggcacgg
    71521 cgatgcatac aggtggtgat ctgcaaagga tcggccgaaa aatggtgctc ggcaaacagc
    71581 atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccgcccgac
    71641 aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg
    71701 tgtggcccga cactattgct acgatgttta tccattctta gcgaagctgc ggtagcgagt
    71761 tgcgttttta actcaccgta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc
    71821 agttttttcc gcgccgccag ttccaccagc cgttcagcat cgcgcagatt ttctgccagc
    71881 ggtttatcga cacagacatg tacacccgca ttgagtaacg tactgaccac gtcaaagtgg
    71941 ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa
    72001 tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac
    72061 caggctcctt gtaacatcca gtcagacgct gccgccagca ccggtaacca cgctttttgc
    72121 gcaatgccgc ctaatcccac aacgccgata cgtaattttt tcactttaat ctcccagatg
    72181 ggccagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca gggcttcgac
    72241 gcgggcctgt aaatcaccat caaccgcgtt cgacatatcc gttaccgccg gctgatcctc
    72301 tacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag
    72361 acgcaccaca aaaggaccat cttcgcgatt tgccagttgt tccagcgtcg actccacttc
    72421 cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg
    72481 ggcaccacgc aataacaacg tggtgattaa cgccacttcc gctgcgctca gtttcagatc
    72541 gccaaattct gaattacaaa aacgttgttc atatttggtg acccgattac caaagccact
    72601 caccgtgcgc agataatggc gtttgaccag attatccagt tgctcctgca cttcagattc
    72661 actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga ctacaccatt
    72721 gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc aaccaatcac
    72781 gcgagcttca agggcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc
    72841 agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat
    72901 agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt
    72961 tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc tgcatataac
    73021 gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg
    73081 aataaccgag atagcaggca tgaaaagagc cacgaacaac attggaaaaa ttggcaacgc
    73141 caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat
    73201 gaaattcgtt aatcatcccc agcctggcct gccagcctga tggataacag tggctttcgt
    73261 cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgcaagac
    73321 gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt
    73381 ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa
    73441 tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat
    73501 tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa
    73561 aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca
    73621 tcttttattt cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat
    73681 atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga
    73741 tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt attgctctcg
    73801 gtctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt gccgaccgct
    73861 ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga ttcgccacaa
    73921 tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg ggactcggcg
    73981 gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt ccacaacaac
    74041 gttgtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc attggcgcat
    74101 tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggccgttt
    74161 tgtttgtgct gtgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg
    74221 tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct
    74281 atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg atgctgccaa
    74341 ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa atggatgtat gccattgaag
    74401 cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc
    74461 tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg ccggtgggca
    74521 tggtcagcgg tctgcaacaa cttttcaccc tgatttgtct gttttatatc gggtcgatca
    74581 ttgccgagcc tgcgcgtgaa accttaagtg ctttgctggc ggacgcaaga gctcgcggca
    74641 gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt tatatcggtg
    74701 gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc
    74761 tgggaattat tggcatcttc actttccttg cgctgggttg gcagtttagt cagaaacgcg
    74821 caacgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga ctcctccata
    74881 ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact
    74941 gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc gtcgaattac
    75001 atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc
    75061 ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc gttcactatg
    75121 tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc attgaagagt
    75181 ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg
    75241 catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca
    75301 gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg
    75361 atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt
    75421 cggaagatga tattcagcta tttccgctac tgcgtaatct gacgctggta gccggaatca
    75481 actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca caaatcaatt
    75541 tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt cgcgggttgc
    75601 cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt
    75661 caggatattg acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct
    75721 gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc
    75781 gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tatcggttta
    75841 cccggcgtgg ctgacgccca tattgttctg acaaacttga tcagccaaat tggtcgcgaa
    75901 gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc cctgttcggt
    75961 agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa
    76021 ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg
    76081 caaacggtga tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa ttactttaac
    76141 cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa
    76201 ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac ggattaatga
    76261 cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt
    76321 gtgtccggca aaaacattcc ttcagccgga gcatagagat taatgactgc accatcccag
    76381 gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta
    76441 aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg
    76501 gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc
    76561 gtacctgccg ggcacgattt caccccattg atgacctgtt atttaacaga ttcgctggat
    76621 cctaatgagc tggagcgcgg atttaatgaa ggcgtgttca ccgctgcaaa actttacccg
    76681 gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat catgccggta
    76741 cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt gacacatgca
    76801 gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc
    76861 cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga tgctgccgac
    76921 tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca tctgatgttt
    76981 aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgttt acccatcctc
    77041 aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt taatcgagta
    77101 ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag ttgcggctgc
    77161 gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg
    77221 aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt ctatgggttg
    77281 ccggtcaacg acacattcat cgaactggta cgtgaagagc aacaggttgc tgaaagcatc
    77341 gcactgactg atgacaccct ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt
    77401 aaacaataaa aatcgtagct tcctgttgtc attaggttat ttaacctgta taaataacca
    77461 gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt
    77521 gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt
    77581 tcctgataat gaaggccacg tatcggtacg ttatgccgca gcgaataatt tatcggttat
    77641 tggcgcaaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag
    77701 cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccgggtcgcc
    77761 ccggcttttt tattttactt acgaattttg agtacaaaat ttctacactt cctcaaaata
    77821 attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt
    77881 aaaagcagaa atttatctgt tccccccttt aaaaattcgg tgaataccct tacttattgg
    77941 tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca
    78001 cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag
    78061 ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga
    78121 ccccaaataa gtccgagcag gaagggactg aagttggtca aacgctctgg ttaaccactg
    78181 atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg
    78241 atttccaggt aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca
    78301 cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct
    78361 taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt
    78421 ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg
    78481 acgcccatat gccaccgcat caacacggca gccaccacgg cgatacgcga ttaattcgcc
    78541 atgcttatgg tgaaggcgaa aagtatgtcc cgctggtcct ccgcgcgcaa acgctgtggg
    78601 atgaactttc ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc
    78661 tcggcccggc tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac
    78721 tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc
    78781 cggacaacta catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga
    78841 ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg
    78901 tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggttgac ggtgagtatc
    78961 aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc
    79021 ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc tatagcgtga
    79081 agaataaatt cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc
    79141 cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag
    79201 cggatgaacg tgttccgttt gcggaagtgg tcagcgatgg ttcggaagcc ttcccgttct
    79261 tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata
    79321 attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca
    79381 ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg
    79441 attttgcgca agacaaaaaa agcgattttg atttgacgcc attcaggctt tcccgcttcc
    79501 aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg
    79561 gctgttgcac tatctc
//
LOCUS       DAJVXP010000004        74366 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVXP010000004 DAJVXP010000000
VERSION     DAJVXP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.004"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            71..1015
                     /locus_tag="OQS10_000268"
                     /pseudo
     CDS             71..1015
                     /locus_tag="OQS10_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="OQS10_000269"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="OQS10_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS2291407.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="OQS10_000270"
     CDS             1797..1904
                     /locus_tag="OQS10_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS2291408.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="OQS10_000271"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="OQS10_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS2291409.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="OQS10_000272"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="OQS10_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS2291410.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="OQS10_000273"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="OQS10_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS2291411.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="OQS10_000274"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="OQS10_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS2291412.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="OQS10_000275"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="OQS10_000275"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS2291413.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="OQS10_000276"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="OQS10_000276"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS2291414.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="OQS10_000277"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="OQS10_000277"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS2291415.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="OQS10_000278"
     CDS             complement(11226..11813)
                     /locus_tag="OQS10_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS2291416.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="OQS10_000279"
     CDS             11983..12561
                     /locus_tag="OQS10_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2291417.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="OQS10_000280"
     CDS             complement(12691..13452)
                     /locus_tag="OQS10_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2291418.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="OQS10_000281"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="OQS10_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS2291419.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="OQS10_000282"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="OQS10_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS2291420.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="OQS10_000283"
     CDS             complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="OQS10_000283"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS2291421.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16807..17205
                     /locus_tag="OQS10_000284"
     CDS             16807..17205
                     /locus_tag="OQS10_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS2291422.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17202..17897
                     /locus_tag="OQS10_000285"
     CDS             17202..17897
                     /locus_tag="OQS10_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS2291423.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            18027..18911
                     /gene="cdd"
                     /locus_tag="OQS10_000286"
     CDS             18027..18911
                     /gene="cdd"
                     /locus_tag="OQS10_000286"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS2291424.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            19061..19780
                     /gene="sanA"
                     /locus_tag="OQS10_000287"
     CDS             19061..19780
                     /gene="sanA"
                     /locus_tag="OQS10_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS2291425.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19783..20022
                     /locus_tag="OQS10_000288"
     CDS             19783..20022
                     /locus_tag="OQS10_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS2291426.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20216..21454
                     /gene="preT"
                     /locus_tag="OQS10_000289"
                     /pseudo
     CDS             20216..21454
                     /gene="preT"
                     /locus_tag="OQS10_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21448..22683
                     /gene="preA"
                     /locus_tag="OQS10_000290"
     CDS             21448..22683
                     /gene="preA"
                     /locus_tag="OQS10_000290"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS2291427.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="OQS10_000291"
     CDS             complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="OQS10_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS2291428.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="OQS10_000292"
     CDS             complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="OQS10_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS2291429.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="OQS10_000293"
     CDS             complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="OQS10_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS2291430.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26695..27735)
                     /gene="galS"
                     /locus_tag="OQS10_000294"
                     /pseudo
     CDS             complement(26695..27735)
                     /gene="galS"
                     /locus_tag="OQS10_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27877..29034)
                     /locus_tag="OQS10_000295"
     CDS             complement(27877..29034)
                     /locus_tag="OQS10_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS2291431.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDH
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(29051..29719)
                     /gene="folE"
                     /locus_tag="OQS10_000296"
     CDS             complement(29051..29719)
                     /gene="folE"
                     /locus_tag="OQS10_000296"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS2291432.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29977..30813
                     /gene="yeiG"
                     /locus_tag="OQS10_000297"
     CDS             29977..30813
                     /gene="yeiG"
                     /locus_tag="OQS10_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS2291433.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="OQS10_000298"
     CDS             complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="OQS10_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS2291434.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="OQS10_000299"
     CDS             complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="OQS10_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS2291435.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34802..35683)
                     /locus_tag="OQS10_000300"
     CDS             complement(34802..35683)
                     /locus_tag="OQS10_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2291436.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35782..36831
                     /locus_tag="OQS10_000301"
     CDS             35782..36831
                     /locus_tag="OQS10_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS2291437.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36905..37762
                     /gene="nfo"
                     /locus_tag="OQS10_000302"
     CDS             36905..37762
                     /gene="nfo"
                     /locus_tag="OQS10_000302"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS2291438.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37766..38854
                     /locus_tag="OQS10_000303"
     CDS             37766..38854
                     /locus_tag="OQS10_000303"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS2291439.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38910..40160)
                     /locus_tag="OQS10_000304"
     CDS             complement(38910..40160)
                     /locus_tag="OQS10_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS2291440.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="OQS10_000305"
     CDS             complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="OQS10_000305"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS2291441.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41331..42029
                     /gene="yeiL"
                     /locus_tag="OQS10_000306"
     CDS             41331..42029
                     /gene="yeiL"
                     /locus_tag="OQS10_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCS2291442.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42096..43346)
                     /locus_tag="OQS10_000307"
     CDS             complement(42096..43346)
                     /locus_tag="OQS10_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS2291443.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="OQS10_000308"
     CDS             complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="OQS10_000308"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS2291444.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44366..45307)
                     /locus_tag="OQS10_000309"
     CDS             complement(44366..45307)
                     /locus_tag="OQS10_000309"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS2291445.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="OQS10_000310"
     CDS             complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="OQS10_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS2291446.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="OQS10_000311"
     CDS             complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="OQS10_000311"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS2291447.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="OQS10_000312"
     CDS             complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="OQS10_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS2291448.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49909..51090
                     /gene="setB"
                     /locus_tag="OQS10_000313"
     CDS             49909..51090
                     /gene="setB"
                     /locus_tag="OQS10_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS2291449.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51087..51341)
                     /locus_tag="OQS10_000314"
     CDS             complement(51087..51341)
                     /locus_tag="OQS10_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS2291450.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51496..52068
                     /gene="yeiP"
                     /locus_tag="OQS10_000315"
     CDS             51496..52068
                     /gene="yeiP"
                     /locus_tag="OQS10_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS2291451.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52291..53757
                     /locus_tag="OQS10_000316"
     CDS             52291..53757
                     /locus_tag="OQS10_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS2291452.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53875..54861
                     /gene="yeiR"
                     /locus_tag="OQS10_000317"
     CDS             53875..54861
                     /gene="yeiR"
                     /locus_tag="OQS10_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS2291453.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54900..55613
                     /gene="lpxT"
                     /locus_tag="OQS10_000318"
     CDS             54900..55613
                     /gene="lpxT"
                     /locus_tag="OQS10_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS2291454.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            56025..56591
                     /gene="mepS"
                     /locus_tag="OQS10_000319"
     CDS             56025..56591
                     /gene="mepS"
                     /locus_tag="OQS10_000319"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS2291455.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56772..58328
                     /locus_tag="OQS10_000320"
     CDS             56772..58328
                     /locus_tag="OQS10_000320"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS2291456.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58410..60224
                     /locus_tag="OQS10_000321"
     CDS             58410..60224
                     /locus_tag="OQS10_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2291457.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60225..61319
                     /locus_tag="OQS10_000322"
     CDS             60225..61319
                     /locus_tag="OQS10_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS2291458.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61319..62344
                     /locus_tag="OQS10_000323"
     CDS             61319..62344
                     /locus_tag="OQS10_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2291459.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62346..63935
                     /gene="yejF"
                     /locus_tag="OQS10_000324"
     CDS             62346..63935
                     /gene="yejF"
                     /locus_tag="OQS10_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS2291460.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63939..64283)
                     /locus_tag="OQS10_000325"
     CDS             complement(63939..64283)
                     /locus_tag="OQS10_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291461.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64616..65736)
                     /locus_tag="OQS10_000326"
                     /pseudo
     CDS             complement(64616..65736)
                     /locus_tag="OQS10_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="OQS10_000327"
     CDS             complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="OQS10_000327"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS2291462.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66609..68369
                     /locus_tag="OQS10_000328"
     CDS             66609..68369
                     /locus_tag="OQS10_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS2291463.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68494..68778
                     /gene="rplY"
                     /locus_tag="OQS10_000329"
     CDS             68494..68778
                     /gene="rplY"
                     /locus_tag="OQS10_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS2291464.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="OQS10_000330"
     CDS             complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="OQS10_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS2291465.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70106..70333
                     /locus_tag="OQS10_000331"
     CDS             70106..70333
                     /locus_tag="OQS10_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS2291466.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70353..72113
                     /gene="pbgA"
                     /locus_tag="OQS10_000332"
     CDS             70353..72113
                     /gene="pbgA"
                     /locus_tag="OQS10_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS2291467.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72188..72264
                     /locus_tag="OQS10_000333"
     tRNA            72188..72264
                     /locus_tag="OQS10_000333"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72222..72224,aa:Pro,seq:ggg)
     gene            complement(72367..74366)
                     /gene="yejO"
                     /locus_tag="OQS10_000334"
                     /pseudo
     CDS             complement(72367..74366)
                     /gene="yejO"
                     /locus_tag="OQS10_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18266 a        19589 c        18257 g        18254 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgtaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccagttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgctaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accaacaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcggt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    15361 gggggcggcc aatcgccgcc gccccctgca cccccgggct ctggcgaaca aaatcgccgc
    15421 tgcgcggtgc cctcggctta tcccttacgg ctaccgggtc gggcgcgagg taacatccct
    15481 gtaaaacgcg ccctcagccc acctccatgt gggctgcccc ggccttcagg gaacgcctcg
    15541 gcaattttga cgccaccaaa caaccgtgcg gcctattgat aaagagctaa cacattgtca
    15601 aaaaacatca ctatggtttt ttagagtttc tcgatatcaa ttgcctgaat agcccttgca
    15661 atatcagggg aattattcaa cacccgaaca tgctgaaata attccgttgc ttcatcgtat
    15721 tctttacgca aataactcaa ccactgttta atccgcgcaa cgtgatataa cccggtatcg
    15781 ccctgctttt ccagacgggt atatttttgc agcaaagcaa ccacctccgg ccacggcatt
    15841 cgcggttcgt tatattttac tacccggctc aggttgggaa tattgagcgc cccgcgacca
    15901 atcatcactg cgtcgcagcc gctgatcgca atgcattgtt gcgcgctctg ccagtcccag
    15961 atttcgccgt tggcaatcac cggaatattc agccgctggc gaatctcgcc aatcgcctgc
    16021 cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc gtccatgcac caccagctcc
    16081 gtagcaccag cctgttgaac cgcatcggcg atttcaaatt tcttctcacc gctgtcccag
    16141 cccagacgca ctttcacgct gacgggcaaa tgcgccggta cagcttcacg catcgctttt
    16201 gcaccctggt agatgagttc aggatctttg agtaacgtcg ccccgccgcc gctgccgtta
    16261 accgttttcg acgggcagcc gcaattgaga tccacgcccc aggaacctaa ttccaccgca
    16321 cgggcggcgt tctctgccag ccattgtggg aactgaccta acaactgaac gcgcaccagc
    16381 gtaccggatg gtgtccggct ggcgttttgt agctcagggc aaatgcgatg aaagactttt
    16441 accggcagca gttgatccac cacgcggaca aactcggtga tgcacagatc gtagtcgtta
    16501 acttcggtca gcaattctcg caccagagag tcaagcacac cctccatcgg agccagtaac
    16561 acacgcatat cttcacccgg aaaaaaatga ggcgctatgt tagcgcctat ggtcaacgga
    16621 ttaaaggtct gcaaagaggg attgctgcaa actgtaatcc ctctctatgc ttgatgtatg
    16681 cctgagttcg ttgcgcattg cgtaatgccg gggagcgaaa aaataagcgc tgatttctta
    16741 atgtgatcgg tagcacgttt tacgaattaa ttgtatgatg aatccatctc atctggggtg
    16801 ttgattatga gtaagacact gaacattatc tggcaatatt tacgcgcttt cgtcctgatt
    16861 tatgcctgcc tgtatgcagg cattttcatt gcttccctgc taccggtaac cattccgggc
    16921 agcatcatcg ggatgctgat cctgtttgtc ctgctggcct tgcaaattct tccggcaaaa
    16981 tgggtcaatc cggggtgcta cgtactgatt cgctatatgg cgctattgtt tgtgccgatt
    17041 ggcgtaggcg tcatgcaata ttttgatttg ctccgcgcac agtttggccc ggtagtggtt
    17101 tcctgtgcag tcagtacgct ggtggttttt ctggtggtga gctggagttc acaactggta
    17161 cacggtgaac gtaaagtcgt aggtcagaaa ggatcagaag aatgatggcg aatatctggt
    17221 ggtcattacc gctgacgttg attgtctttt ttgccgcccg caaactggcg gcacggtata
    17281 agtttcccct gcttaacccg ttgctggtag caatggtggt gatcattcct tttttaatgc
    17341 tgaccggcat ctcttacgac agctacttta aaggtagcga agtgctaaac gatctgctgc
    17401 aaccggcggt cgtcgcgctg gcctatcctt tatatgagca gctacaccag atccgcgcgc
    17461 gctggaaatc gatcatcacc atctgtttta tcggcagcgt ggttgcgatg gtgacgggga
    17521 cttccgtggc attgttgatg ggcgcttcac cggaaatcgc cgcgtcaatc ctgccgaaat
    17581 cagtcaccac gcctattgca atggcggttg gcggcagtat tggcggtatt ccggcaatca
    17641 gcgcagtttg cgtgattttc gtcggcatcc tcggcgcggt atttggccat acattgctta
    17701 atgcgatgcg tattcgtacc aaagctgcgc gcggtctggc aatggggaca gcctcgcacg
    17761 ccctcggtac ggcgcgctgc gccgagctgg attatcagga aggtgcattt agttcgctgg
    17821 cgctggtgtt atgcgggata attacttcgc tgatcgcacc gttccttttc ccgattattc
    17881 tggcagtaat gggctaaaat ttgcgatgcg tcgcgcattt ttgatgtaag tttcacgcgt
    17941 tgcataatta atgagattca gatcacatat aaagccataa cgggttcgta aactgttatc
    18001 ccattacatg attatgaggc aacgccatgc atccacgttt tcaaaccgct tttgcccaac
    18061 ttgcggataa cttgcaatct gcactggaac ctattctggc agacaagtac ttccccgctt
    18121 tgttgaccgg ggagcaagtc tcatcgctga agagcgcaac ggggctggac gaagacgcgc
    18181 tggcattcgc actacttcca ctggcggcgg cctgtgcgcg tacgccattg tcgaatttta
    18241 atgttggcgc aattgcgcgc ggtgtgagcg gaacctggta tttcggtgcc aatatggaat
    18301 ttattggtgc aacaatgcag caaaccgttc atgccgaaca aagcgcgatc agccacgcct
    18361 ggttgagtgg tgaaaaagcg cttgcagcca tcaccgttaa ctatacgcct tgtggtcact
    18421 gccgtcagtt tatgaatgaa ctgaacagcg gtctggatct gcgtattcat ctgccgggcc
    18481 gcgaggcaca cgcgctgcgt gactatctgc cggatgcctt tgggccgaaa gatctggaga
    18541 ttaaaacgct gctgatggac gaacaggatc acggctatgc gctgacgggt gatgcgcttt
    18601 ctcaggcagc gattgcggcg gcaaaccgtt cgcacatgcc ttacagtaag tcgccaagcg
    18661 gtgtcgcgct ggaatgtaaa gacggtcgta ttttcagcgg cagctacgct gaaaacgccg
    18721 cattcaaccc gactctgcca ccgttgcagg gggcgttaat tctgttgaat ctcaagggtt
    18781 atgattaccc ggatatccag cgcgcggttc tggcagaaaa agccgatgcg ccgttgattc
    18841 agtgggatgc cacctccgca acgctgaaag ctctcggctg tcacagtatc gaccgcgtgc
    18901 ttctcgctta agcctggtgc cggatgcggc gtgaacgcct tgtccggctt gccagccctc
    18961 tcttggtgtc gaaattcccg gcaaacagtt tgccgtttct tgcgcaaaac cagcgggtaa
    19021 agtagcctga tggaaatttt ccttagatcg agtctcctgc atgttaaagc gcgtgttcct
    19081 cagcctgtta gtcctgatcg gcttgctgct gttgactgtg ctcggcctcg atcgctggat
    19141 gagctggaaa accgcgcctt atatctacga cgaattgcag gatctcccct accgccaggt
    19201 cggcgtggtg ctcggcacag caaaatatta tcgtactggc gtaattaatc agtattatcg
    19261 ctaccgcatt caaggagcga ttaatgccta taacagcggt aaggtaaatt atctattact
    19321 gagcggcgat aacgcattgc aaagttataa tgagccgatg accatgcgca aagatttaat
    19381 cgctgctggt gtcgacccat cagatattgt tctcgattac gcaggctttc gtacgctgga
    19441 ttccatcgtg cgtacacgca aagtgttcga tactaatgat ttcattatta tcacccaacg
    19501 tttccactgt gagcgggcat tatttattgc gctgcatatg gggattcagg ctcagtgtta
    19561 tgccgtaccg tcaccgaaag atatgctgtc agtacgtatt cgcgaatttg ccgcccgttt
    19621 cggtgcgctg gctgaccttt atatttttag acgtgaaccg cgttttttag ggccgctggt
    19681 ccctattccg gctatgcacc aggtgccgga agatgcgcag gggtatcccg ccgtcacacc
    19741 cgaacagtta cttgaattac aaaagaaaca aggaaagtag ttatggatgt acagcagttt
    19801 tttgtcgttg ccgttttttt ccttatcccg atattttgtt tccgcgaagc atggaaaggc
    19861 tggcgcgcag gcgcgattga taaacgggtt aaaaatgcac cggaaccggt gtatgtctgg
    19921 cgagcaaaaa atcccggact ctttttcgct tatatggtgg catatatcgg cttcggaatt
    19981 ttatctatcg gcatgattgt ttatcttatt ttctatcgtt aatattttct cttttctgtt
    20041 aattattgag aataattatt acttcactca ataagctgcg gatataacat tctgaaccgc
    20101 agctatttgt gaatcctttc acagtttaaa tttccccaca cgcttagcct taatatcagc
    20161 acgcaatatt tatttagcgt tcgccttaat tactcatagc attaaggaag atcacatgcc
    20221 gcaacaaaat tatctggatg aactcactcc ggcttttacg cctttactgg cgattaaaga
    20281 agcctctcgc tgtttattat gtcatgacgc tccctgtagt caggcttgcc cggcacagac
    20341 cgatccgggg aaatttattc gctcaatcta ctttcgtaat tttaaaggcg ctgccgagac
    20401 aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga gtgtgcccga cggagaaatt
    20461 atgccaaagc ggttgtaccc gtgccggtgt tgatgctccc attgatatcg gccgcttaca
    20521 gcgttttgtt actgattttg aacaacaaac cgggatggaa atttatcagc ccggtactaa
    20581 aacgctcggc aaagtcgcga ttattggcgc aggtcctgcc ggattgcagg ccagtgtgac
    20641 actgacaaac cagggttatg acgtcacgat ctatgagaaa gaagcacaac ccggtggctg
    20701 attgcgtaac ggcattccgc aattccggtt accgcaatca gtactggatg cagagatcgc
    20761 ccgtattgaa aaaatgggcg tgaccattaa gtgcaacaac gaagtcggta acacactcac
    20821 ccttgagcag ctgaaagcag aaaaccgcgc ggtactggtc accgtggggt tatcaagcgg
    20881 ttccgggcta ccgctgtttg agcatagtga cgttgagatt gccgtcgact tcttgcaacg
    20941 tgcacgacag gcgcaaggcg atatcagcat tccacaaagc gcattaatta tcggcggcgg
    21001 tgatgtcgcg atggacgtag ccagcacgct gaaagttctc ggctgtcagg cggtaacttg
    21061 cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa aaagagttta ccagcgcccg
    21121 ggagctgggc gtttcgatca tcgatggatt cacgccagta gccgtggaag gcaataaagt
    21181 cacgtttaag catgtacggt tatcgggcga actgacgatg gcggcagata aaattattct
    21241 cgccgtcggt cagcatgcca gactagatgc ctttgcggag ttagagccgc agcgtaacac
    21301 catcaaaaca caaaattacc agacccgcga cccgcaagtc tttgctgctg gcgatattgt
    21361 tgagggagac aaaaccgtgg tctatgccgt gaaaaccggg aaagaagccg ccgaggcgat
    21421 tcatcactat ttagagggag cttgctcatg ttaacgaaag atctttcgat tactttttgc
    21481 ggcgtgaagt ttcccaaccc gttctgcctc tcttcttcgc cggtaggcaa ctgctatgag
    21541 atgtgtgcca aagcctacga cacaggttgg ggcggtgtgg tgtttaaaac gatcggcttt
    21601 tttatcgcca acgaagtctc gccgcgtttt gatcatctgg tgaaagaaga taccggtttt
    21661 atcggcttca aaaatatgga gcagattgcc gagcatccgc tggaggagaa tctggccgcc
    21721 ctgcgtcggc tgaaagaaga ttacccggac aaagtattga tcgcttcgat catgggggaa
    21781 aatgagcagc aatgggagga gctggcgcgc ctggtgcaag aagctggcgc ggatatgatc
    21841 gagtgtaact tctcctgtcc gcaaatgact tctcatgcga tgggtagcga tgtcgggcaa
    21901 agcccggagc tggtagaaaa atattgtcgg gcagtgaaac ggggttccac gctgccaatg
    21961 ctggcgaaga tgacgccgaa tatcggtgat atgtgcgaag tggcgctggc ggcgaagcgc
    22021 ggcggcgcag atggcattgc ggcgattaac accgttaaat ccatcaccaa tatcgatctt
    22081 aatcagaaaa tcggtatgcc gatcgttaac ggaaaatcaa gtatttccgg atattccggt
    22141 aaagcggtaa aaccgatcgc cctgcgcttc attcagcaaa tgcgcaccca tccagaactg
    22201 cgcgatttcc caatcagcgg tatcggcggc attgaaacct gggaggatgc ggcggagttt
    22261 ttattgctcg gcgcagcaac gttacaggtc accaccggca ttatgcagta cggctatcgc
    22321 atcgtagaag atatggcgag cgggttatcg cattatctcg ccgatcaggg atttgattcg
    22381 ttgcaggaga tggtaggtct ggcgaataac aatattgtcc cggcggaaga tttagaccgc
    22441 agttatattg tctatccccg tatcaatctt gacaaatgtg ttggctgtgg acgctgttat
    22501 atttcctgtt acgacggcgg tcaccaggcg atggaatgga gcgagaaaac ccgcacaccg
    22561 cattgtaata ccgagaaatg tgtgggttgt ctgctttgtg gtcacgtctg cccggtgggt
    22621 tgtattgagc tcggggaagt gaagtttaag aaaggcgaga aagaacaccc ggtaacgttg
    22681 tagaacccgc gtcacatccg gcatttggtt cctgttgccg gatgcggcgt gaacgcctta
    22741 tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg aatgcggacc
    22801 acattcacat catttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat
    22861 aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata
    22921 gttgatgacg gtaaaaataa tcaccccggt caccacgcca atcaccgtcc ccacaccgcc
    22981 gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc
    23041 gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa
    23101 cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt
    23161 tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca
    23221 caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa
    23281 actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat
    23341 tggcgacgcc ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt
    23401 gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa
    23461 accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc
    23521 catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac
    23581 tgccgccagc cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc
    23641 tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt
    23701 caacagactt aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac
    23761 gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat
    23821 gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt
    23881 tgtttttgta tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt
    23941 tatccctaac aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc
    24001 cagttcggca attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg
    24061 ttcatcgagc attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg
    24121 ctgattacca ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat
    24181 cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt
    24241 atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata
    24301 aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta tggcttcgtt
    24361 ggcattatgg ttattgatct gtttgccgtg caacgtaatg gtgccagccg atttctcgcg
    24421 aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc
    24481 gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga
    24541 cgttaggtta cgcacctcga ggatgacttc gcccggcttg ttttctttgt caggaaaacg
    24601 ctgattaaga gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag
    24661 cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa
    24721 gatttcttcc attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg
    24781 aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat
    24841 aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga
    24901 aacggataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat
    24961 tgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg
    25021 ccccagccac atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac
    25081 catcgaaata ccattttcca gtgcctcttt ggcggaatgg aaatcgatct ctttaccctg
    25141 gaataaaatg gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga
    25201 ttttcctgca ccgttttccc ccattaatgc atggatagaa tgtggccgga cttttaaatt
    25261 aacgttatca agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa
    25321 gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac
    25381 aataagggcg cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag
    25441 gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt
    25501 ggtgccatca gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt
    25561 cgcctggttg ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc
    25621 cagcgcttct ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt
    25681 cagtgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt
    25741 gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtgtccca
    25801 cattgcggta tctaactgta actgttcagt tttgatgcct ttatcgttca attctttaat
    25861 cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcagtacgaa
    25921 ctgaatctga ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat
    25981 caaatcgccc tgaataatgc cggactcttt ggagtcagtg ccaacgtagt aggctttgtc
    26041 gtagctatcc agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg
    26101 cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag
    26161 tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt
    26221 ctgagaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc
    26281 cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt
    26341 atcagcagcg tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa
    26401 caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg
    26461 ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac ttttttaaat
    26521 tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat
    26581 tacctcggtg aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa
    26641 tcatagctat cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat
    26701 cgcctgattt gttgagttag tgatcgccgc cgcattctgg cgcgttgcga cagaatggcg
    26761 acgcactaac gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc
    26821 ctgcaaggcc agttcggtgg ctaatttagc cattgaagca atgggataac gcacggtcgt
    26881 taattgcggg tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg
    26941 taacggaatc gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc
    27001 gttataagca aatatagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc
    27061 ctcaccgccc ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc
    27121 ctgctctttc aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg
    27181 actggaagaa agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat
    27241 tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg
    27301 gttgattaac accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa
    27361 tgcttttgag tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg
    27421 acgctctttt tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg
    27481 agcgactaga tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac
    27541 caccacgcca atggtgtcgc taacctgagt tgccagcgcc tgggcattgg cgtttggccg
    27601 ataatccagc tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt
    27661 gctgttattg agcacccggg aaaccgttgc cacagagacg ccagcctaac gcgctacatc
    27721 acgaatggtg atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc
    27781 ggctattttg tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg
    27841 ttacagccgt tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc
    27901 cgttccggca gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa
    27961 gtaacgcagc cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc
    28021 cagcagctcc aggtggtcaa aatgcataaa caaaccgagg tggtaaaaaa gcatggtaca
    28081 aatcagcgtt tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag
    28141 caccagctta aagcggctca attgcggcca gaagccataa aacagcgacg catagccaat
    28201 cgcctgaaac ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata
    28261 cgcccagtcc agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc
    28321 caccagcaca aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc
    28381 gctgcgcatc agcgccgcac caatgagcat catccctgcc agttgccagc catactgtgc
    28441 gcccagtgcc agtaaactgt tgccaacacc atcggcacga ttactgatcg cttcaacgcc
    28501 gccgtgaagc ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggtgcg
    28561 gctggtctgg ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata
    28621 aagcatgacg cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca
    28681 gcagattaag cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc
    28741 gtgaataaaa cccagcaaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg
    28801 gggcagcaac atttgcaggc ccgcaccaaa cagcagcgca aaaagcgtga ggaatttcac
    28861 ctggccgatg agatcgagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcgccgta
    28921 ccaggcggga ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag
    28981 gatccccaga atggcgacgc cgcgaacaaa atcgagcgtt acgttgcgct ccatggttcc
    29041 tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac
    29101 tggatttgaa caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc
    29161 cacgcgcttt cacgcagtaa tgcaccgcat caatggagac agccacgtta ttggtgccca
    29221 gcagcgtttg tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct
    29281 gggcaaagaa ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga
    29341 tataagccac cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca
    29401 gagtgatatc gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga
    29461 ttttcgggaa gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga
    29521 tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga
    29581 tttcggtcat atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg
    29641 gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt
    29701 ctttactgag tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg
    29761 ggggcaaagt tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa
    29821 tccagtattc acagcgataa ttattgtaat tgccgctgac tttcatcagc agatgttaaa
    29881 acatcgttat gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga
    29941 tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc
    30001 tgttttgaag gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg
    30061 acgttcagta tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg
    30121 ctttccggat taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg
    30181 gcggcagaac tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt
    30241 gccaacgacg atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa
    30301 ccgccgtggg cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg
    30361 gttcagtcgc aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt
    30421 cacggtgcgc tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt
    30481 gcgccaattg tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cacctattta
    30541 ggtgaggaca aaaatgcatg gctggaatgg gatagttgcg cgctgatgta tgccagtaac
    30601 gcgcaggatg cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac
    30661 cagttgcaac ctgcggtatt ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg
    30721 cgtattcagc cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac
    30781 ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt
    30841 ctcatcagaa gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt
    30901 agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt
    30961 ctttagtcac ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag
    31021 ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt
    31081 cttccagcgc cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg
    31141 atagcggttt gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg
    31201 acagtttcca ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag
    31261 ctttgttaac gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa
    31321 aaccaacaaa gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga
    31381 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    31441 cttcttcacc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    31501 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    31561 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    31621 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat
    31681 cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca
    31741 caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg
    31801 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    31861 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    31921 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    31981 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    32041 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    32101 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    32161 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    32221 tgccgtaagc tttcattccc agcacaccat caattaatgg tccactggta aagaactgac
    32281 cgttataggt atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac
    32341 ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg
    32401 aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg
    32461 ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttcacgcgtt
    32521 taccgtcgac gagaatcagg gtatagctgc tgtccagacc acgaatacta acgcccttac
    32581 ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat
    32641 tctgtaccgg ttttcgctgc agatcttcct gggtaatgac gctgatactg gcgggtgcat
    32701 ctttaaggtt ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcgtcatcga
    32761 ccgctaacac aggccataca caagaaatag cggacaaaca cagcccgacc cgtacgaaag
    32821 ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa tgggtatcgc
    32881 tcacatcttc tttacgtccc cttgcgtacg gcagcatcgc ggtggactta tttgtatgca
    32941 ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt gattgtagcg
    33001 taacgataat gcaaacgata acaattatca atccaaatgt taaattttat atccgcatga
    33061 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    33121 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    33181 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    33241 ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca
    33301 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    33361 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    33421 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    33481 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacaaaccg gcaatcaccg
    33541 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga caatttagcg aaaatgcgcg
    33601 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    33661 tacctgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    33721 gacccgcgtg ctggaacacc aaggtgaacg gactgacgct gatgtcttta acatcgttac
    33781 gcagcaggct cggatcggta tacggaataa tcaggctgat aatcaggatc gcgaacacat
    33841 agaacaacag gattcgccag aatacctgac gtaccgcgcg tggaatgttt ttcgccggat
    33901 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    33961 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    34021 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    34081 cgatgataaa gacaataact gtcgtgactt tgatcagtga aaaccagtat tccgcttcac
    34141 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    34201 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgtgct gcaaccaagt
    34261 caacggcaat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    34321 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    34381 ccaattcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    34441 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    34501 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    34561 agcccggcgc ttctgtagtt ttagtttcgg aacccataca aaaatgctat ccatcttaaa
    34621 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    34681 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    34741 gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcac
    34801 cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    34861 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggg agagggaccg
    34921 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    34981 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    35041 ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg
    35101 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac
    35161 gagccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    35221 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    35281 gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc aactgcggat
    35341 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    35401 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    35461 caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    35521 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    35581 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    35641 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    35701 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    35761 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    35821 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    35881 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    35941 gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg
    36001 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    36061 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc
    36121 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    36181 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    36241 tcggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    36301 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    36361 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    36421 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    36481 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    36541 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgtc
    36601 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    36661 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    36721 gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    36781 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    36841 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    36901 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    36961 tcgcgccgtc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    37021 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    37081 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    37141 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    37201 acaactgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    37261 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    37321 tgtaacagcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    37381 acatctcgcg gcgattatcg acggcgtgga agataaattc cgcgtcggcg tctgcattga
    37441 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgaaaaaac
    37501 gttcgctgat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga
    37561 tgcgaaaagt acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    37621 cggtcatgat gcgttccgct ggatcatgca agacgaccgt ttcgacggca ttccgctgat
    37681 ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    37741 aactgaaaaa gcggtggcct gatcgatgaa cgacagggaa aagcagattc ttaaaatttt
    37801 acggcgtaac ccgctgattc aacagaatga aatcgccgat attctgcaaa tcagccgctc
    37861 acgtgttgcc gctcatatta tggatttgat gcgcaagggt ttgattaaag gcaaaggata
    37921 tatccttacc gagcaggatt actgtgtggt tgtgggtgca atcaatatgg atattcgcgg
    37981 aatggcggat atccgttacc cgcaagcagc ctctcatcca ggttctgttc actgttccgc
    38041 aggcggcgtc ggacgtaata tcgcgcataa cctggctctg cttggtcggg atgtgcattt
    38101 aatatcagct attggcaacg atttttacgg cgagacatta ctggaagaaa cgcgccgcgc
    38161 gggcgtgaac gtttcaaact gcattcgtct gcatggacac agcactgcaa cttatctcgc
    38221 tatcgctaat aagcaggaag aaactatcct ggcaatcaat gacacacata ttctgcaaca
    38281 gctaacgcct caactgctca atacttcacg ggacttgatt cgccatgctg gtgtggtgct
    38341 ggctgattgt aatctcacac ctgaagccct ggagtgggtc tttaccattg ctgatgaaat
    38401 tccgatgttt gtcgataccg tttccgaatt taaagcgaac aaagtaaaaa actggtacag
    38461 ccggatccac actctgaaac caacacaaaa tgaactggaa attttgtggg ggcaaccgat
    38521 taaagacgac aacgatcgca tcagagctgt aaattcgctg catcaacaag gtgttaaacg
    38581 catttttgtt tatctgaaag atgaatctgt attttgcagc gataaggatg gagaacaatt
    38641 tttgctgacc gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat
    38701 ggcgggcctg gtatatagct ttcttgaagg atacagtttc cgcgacagcg cccgttttgc
    38761 ggtagcctgc gcggcaattt cgcgcgccag cggcagctta aacaacccta ccctgtctgc
    38821 cgataacgcg ctttcattag tgccaatggt gtaacaatgt tgccggattc gacgcttgac
    38881 cgcatcttat ccggctcata aattgcgcac tacgctaaac caataaagaa tcctgcaata
    38941 gtagcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagttgggcg
    39001 atttccggcg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa
    39061 ccaaagttag cgaaaccgca caacgcgaaa gaaataatcg ccacggtttt agcatccaga
    39121 gtgccacccg tttgcagata tggcgagaaa ttgagataag cgacaaattc attgatcgcc
    39181 agcttctgcc caatcaaact cccggcaaga tttgcatcac tccagtcaac ccccatcacc
    39241 cacgccaatg gggccaacag gtaacctaaa atcgactcca gcgaagcatg ttcaaaacca
    39301 aaccagccgc caacaccgcc gataataccg ttaatcaacg caattattgc aacaaatgcc
    39361 ataacaactg tcgctacacc ggcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg
    39421 gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa agaaacctgc
    39481 gaagattccg tagccgggct taacaggcgg gcaaacaaga tcccgccagg gatcgccatt
    39541 aacgatgccg ccagcaaata ttccacaggt acgcccagtg cggcgtaacc aatcattgtc
    39601 gaaccagcaa tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga
    39661 cgatcgataa agggtttgac gatcgccgga atttcgtttt gcccgaggaa aatggtggtg
    39721 accgcgacga atgactcgat cttgctgata tttaaagctt tctggaagat accgccgaga
    39781 atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag tgcagtgacg
    39841 aagataattg ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaataaggta
    39901 tccattttcg gtccgaccag agaaccgaag ataaatgcgc tacctgcgtc gctgtacgcc
    39961 atcactttgt ggacgccaaa agcgactttt tctgcgaccc aacgccctgg cggtaaccaa
    40021 agcataatcc cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg
    40081 atcttcttct tgtttactga cagtaaaaac gcaattgcca gcagtaccac cattcccaga
    40141 acacttctca tgacatccat agtgattatc tcttcatgtc attttgggcg acggctttct
    40201 gcccatcaga aatagccggg cctgcatctt gcgatactgt cccggcttgt tatcaacact
    40261 taatgggttt ttatgtagcc gcgtacgcac tcttcgacta atccccagaa ccagtcggta
    40321 tcaatagtga tgccgacttt agtattggcg ggcttgccaa gaacgcccag ctcgtcgcag
    40381 acggtacgcc catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgggtt
    40441 ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca
    40501 gccaggccgt agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacatc
    40561 tctccggcag ggccgcctgc cctttccatt cgtgcaatta cgtccggggt gcaaacggtc
    40621 tggttggtaa gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgt
    40681 gcggcttccg ggtcggcaaa gatattgaac tcggcagatg gggtgaagtt gcctgtaccg
    40741 taagcgccgc ccattagcac aatttcacgg attttgggca ggattgcggg ttgcatacgc
    40801 attgccaccg cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt
    40861 gccataaggg tatcaatgat gtatttcacc gcgtgggtac tttctgcctg gcgggtcagc
    40921 ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt
    40981 tgacgcataa tgggttgcgg catccccgca taaaccggaa cattaatctc cagtttctgg
    41041 caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc aacaatggtg
    41101 atacctaata aatcaatcgc cggatgtttc gccgccatca ttatagcaat agcatcatca
    41161 tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc
    41221 cagttgcgtt attttttcta cagcaacgaa atttcgatcg ggccgatgat tgaatcttaa
    41281 caacagcgta cgaatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa
    41341 tccacaataa tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt
    41401 taacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc
    41461 aggaagggca acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg
    41521 ccacgctagc taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg
    41581 gcgagattga gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt
    41641 gttggtgcct tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat
    41701 ttttacgaaa actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa
    41761 ctcagaatca atcatttccg ttagtgaatc gcctggcagc atttatatta ctctcgcagg
    41821 aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc
    41881 gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt aaaagcaaga
    41941 aagggtatct aattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg
    42001 agaataaatt ctccgggatg atgcagtaaa aattattggc aatagcacga ttgccggatg
    42061 caacgcttaa taaggcgtac atccggcaat gaaactcaag ctaaaccaat aaagaacccg
    42121 gcaatggtcg cgctcatcag gttggaaagc gtcgccgccg ccagcgcccg taaaccaagc
    42181 tgggcgattt ccggcgcgcg ttttggcgaa atagccgaaa acgctccaac gacaacaccg
    42241 atagaaccaa agtttgcaaa cccacagagg gcgaacgaga taatggcaat ggtcttgaca
    42301 tccagcgtgc cgctcgtttg cagatagggc gagaaattga gataagcaac aaactcatta
    42361 atcgccagtt tctgcccaat caaacttccg gcaagatttg catcactcca gtccaccccc
    42421 ataatccatg ccaacggggc cagcacatag ccgaaaatgc tttccagaga gacattggca
    42481 aaaccaaacc agccgccaac tccaccaata atgccgttga tcagcgcaat gatcgctaca
    42541 aatgccatta ccaccgtcgc cacaccggcg gcgattttca gcccggtcat tgcaccgttc
    42601 gccgcagctt caataatgct ttttggcggt gtttcgctaa acgacagatt ttcaaacgta
    42661 acttgcgaag gttccgtcgc cggactaaga atacgtgcaa acaggatacc gccaggaata
    42721 gccatcagcg aagcggctaa caaatagtca atcggcaccc ccattccggc atagccaatc
    42781 atcatcgaac ccgcaataga cgccatgccg ctacaaatag ccgtaaatag ctcgttgcga
    42841 ttcatgcgat caataaaagg tttaacgatc gccgggatct cattttgccc gaggaaaata
    42901 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg aaaaatactg
    42961 ccaagaatac gaatcagcag ccccattacg ccaatgtagt acagcaagct gataagcgcg
    43021 gtaacgaaaa taatcgccgg gagtacgcga aaggcgaaga taaagcccgc accgtcaaac
    43081 agaacatcca ttttgggacc gaccagcgaa ccaaaaataa aggcacttcc tgcgtcactg
    43141 taggacatca ctttatggac gcctaatgcc gcctgctcta ctgcccattt tcccggggga
    43201 aaatagagca tgatgccacc aatggcaatt tgcagcagca gcgcagcgcc aaccgtgcgc
    43261 aagctgatgc tctttttatt taccgacaac acaaaggcaa ttgccagcaa taccaccatc
    43321 cccgcaacac ttctcattat atccataatg attttccctt catgccagta aacccggcgt
    43381 cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc cgccagaaat
    43441 taacccacga gacgctgata ttctttggca atttcgctcg ccagaatggc gttattaaag
    43501 accagttgaa tattggattt caggctatca ccgccggtta gctccgcaac acgcgccagc
    43561 aggaacggtg tactctcttt cccaataaca ccctgttctt cggcttcagc aacggcttga
    43621 tcaattgctg cattgatggt ttcttctggc atcgcaaact gttccgggat cgggttcgct
    43681 accaccaggc cgccgttcaa accggtttgc cacttcactg ccatcgcgcg agcaatttcg
    43741 cgggcactgt caagacgaat gctgacttca aatgggctgg ttcggcagaa aaacgcaggc
    43801 agcgcttttg tctgataacc aattaacggt acaccgaagg tttctaaata ttctgttgtt
    43861 aatcctaaat cgagaataga ttttgccccc gcacaaacaa cagtgacatt ggtatttgcc
    43921 agttcctgta aatcggcaga aatatcgaag gtatgttccg ctccgcgatg cacaccacca
    43981 atacctccgg ttgcaaaaac tttaatccca gccagtgctg caataatcat ggttgatgca
    44041 acggtagtcg cgccattttt ccctgcagca acgacaaaag gtaaatcacg acgactaact
    44101 ttcgtcacgt tatgtccttc acggcccagc aattcgattt cttctttact taagcccact
    44161 ttcatcacac cgccaataat ggcgatagtg gctggtactg cgccctgttt acgaattgtt
    44221 tcttctactt caattgcggt ttgggcattt tgtgggaatg gcattccgtg agaaattatg
    44281 gtcgattcca gcgcaacaac cggtttttta ttttttaaag catcctgtac ttcagcggat
    44341 atttgtaata attcagggga cagagttaat tcagacattc tgtgttctcc actaacgatc
    44401 taacattggc aattgataat tcagggttgt tggtatattc acaggcaagt gccattgacg
    44461 aacatccctg cgcaaatcga acagaatcga taaacggcat accgtctacc caacaggagg
    44521 ccagccccgc catcatggcg tcgcctgccc cagtaacatt gatgacattc gttttaatag
    44581 gggccgacca cccactttca ccgttgatat cactgtagta aacgccatcc cctcccatgc
    44641 tcagcaccaa gcggttcagg ccgtgttgat gaaaccaggc ggcgaccttt gcgacatctt
    44701 cacgtccgga aagtgcaatt ccgctcaacg tttcggcttc aagacggtta ggttttaacg
    44761 tatggatttt gctcagatga tcgcggattt tgacgcattt ccatgccgat accggatcaa
    44821 taaatacagg cgtttccccg ctattttcca gaacccagac cagcacctct tcactaaggt
    44881 tgcaatccgc gacaataacc tttgcaccgc gaataaactc ctgatgctgg gcaagaaatt
    44941 ctgcagaaat acagtcacta atactcatat catttatcgc gacaagcatt tctcctgtat
    45001 tatcgagcag tgataaatag ctggatgtat tctcacccgg aacaattaag catttatcga
    45061 cataaacacc ggattgatta gtttgcgcca gtaatgattg cccataaaaa tcaccaccta
    45121 ctgcacttaa aagccaggca tttttcccca gtaacgccag attatgagcg atattccttc
    45181 ccacgccacc tggggtaaat ttaatttttc cgggattcga atccgcataa ttcaatggat
    45241 gatgtgaata cccagccaca tcaatattcg ccgaaccaat aattactatg tagtcctttt
    45301 cgcgcatagc acgccccctg gcaataaatt gccaacgaag aaattgggta aaatagccat
    45361 tacagtcaat aagactatta gagcacgtgt tcagatttaa acacatgttc ataataagca
    45421 tatttaacgt aatgtaaatc gatcagaaaa aacgaaatcg gccagatatg aaggcggtca
    45481 caaagtttgt ctatttatgc agcaacaaaa gagaaagttg tgatgtgtaa cacacaatat
    45541 ggcaggcaag aaaaagggca gagatgtttc tgccctccag attgttgaca acgcgcgctt
    45601 tgttcatcct ggatgtggcg taaacgcctt atccgaccta caaaagcgca aaaaattcaa
    45661 tatattgcag ggttcatgta ggtctgataa gcatagcgca tcaggcaatt ttgcgtttgt
    45721 catcatcggg gcagagatgt ctcatccccg ataatgaaga taaattatgc gtttttttct
    45781 actatttgtg tttccgggcg tttcaggaag gcataggcca aacccgccac cagcgtaccg
    45841 gcaataattg ctaccaggta acccaatacc ggcgtaatag cgccagggat cagcagaaca
    45901 aacagaccac cgtgtggtgc catcagtttc gcaccaatcg ccattgagat tgcgccagtc
    45961 agcgccccac caacgataca gcacggtagc acacgcatcg gatcacgagc agcaaacgga
    46021 attgcacctt ccgaaataaa gcacagacca agtaccagag cggctttgcc accttcctgc
    46081 tgcgctttgt cgaatttgcg acgcgccacc attgttgcca gacccattgc cagcggtggc
    46141 accatacctg ccgccataat cgccgccatc gggccatagg tttgagtact cagcagaccc
    46201 acaccgaatg cgtacgctgc tttgtttacc ggaccgccca tgtcagtaca catcatgcca
    46261 ccgaggatcg cccccagcag aaccgcattc gcagtcccca tggtctgcag ccagtgagtc
    46321 aacccatcga gaatgccagc aaccggttta ccgattaggt agatcatcgc cagaccgacc
    46381 accagactgg aaattagcgg aatgatcagg atcggtttca gcgcctccat actctgtggc
    46441 agtttcaatt gcgtactgat taacttcgca atgtaaccag ccaggaagcc agcaattata
    46501 ccgccaataa agccagaacc ggtgctgacc gccagcatac cgccaatcag acccggtgtg
    46561 aggcccggac gatcggcaat ggagaaggca atataacctg ccagtaccgg caccatcagc
    46621 gcaaaggctg aaccaccacc aatctgcatc agcgccgctg ccaacgtacc cggctcttta
    46681 aacgcttcga taccaaaagc aaaagaaagc gcgatacaca gaccacctgc aacaaccatc
    46741 ggcagcatgt aagagacgcc cgtcagcaag tgacggtaag cgcctgcact ctctttctta
    46801 ccttcagtgg tcgctgtttg agctttgccc gctggttcat acggcgttgc ttcagcaacc
    46861 gctttatcca gttcctgcgc agttttcttc agcgccagac cggtagaggt gcgatacatc
    46921 ggtttaccag caaatttcgc cagatccact tcgatatctg ccgccacaat caccagatcc
    46981 gctgctgcga cttcttcagg ggtgattgca ttgcccgcgc caacagaacc acgggtttca
    47041 actttcaccc accagccacg ttttttcgct tcggtttcaa tggcttcagc cgccataaag
    47101 gtgtgtgcta cgccagtcgg gcaagcagtc accgcaacta cgcgtttcgg accgctggca
    47161 gcaaccggtg ctgtcgcagc gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca
    47221 ctcaggaaca gctcagggtg cgcaactgcc cgggaaatat cacccagcca gacattttta
    47281 ccgttcagcg cgctgtcatt cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg
    47341 ttcggattgt cgatgatttc cagttttgct tttcgcgccg ccgcgcccag cagggtcttc
    47401 gccatatagg cgcgtgcctg accgagatta gcgtcaataa tcagcagcgt tttcattatg
    47461 cctctcctgc tgtcagttaa aaggttgtaa gtcgacgcgc gccatcattg cggccaactg
    47521 cggacgatcg gtaataccca cattgctttg acttaccgcc agggctgcaa cagctgtcgc
    47581 cagacgcagt gtgtgttcac tggattcacg catcagcaag ccataaatca ggccaccaac
    47641 catagaatcc cctgcgccaa cggtgcttac gacatcgact gacggtggtt tggcgatcca
    47701 ttcgccggag gcattaaccc aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc
    47761 aataccttgt tcacgtagcg catgtgcagc ttcaatcaca tctttcattt caggcagttt
    47821 acggcctgcc cagatttcca actcgcggcg gttaggtttc accagccacg gtgccgcttt
    47881 caaacctgct actaacgctt cacggctact atcaaagata atgcaaggac actgactacg
    47941 caggcgagtc atccagtcgg tgaacgcttc cgggctgacg cctgacggta agcttccgct
    48001 gacacagacc atatcgaact gaccgagcca gctcagagaa tcagtcacaa agcgttccca
    48061 gtcggcgagg gtgacttcaa aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc
    48121 cgtcagctta acgttaattc gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc
    48181 cagctcgctg aacagttgct gaaaaccatc ctgattgtct ttacccagga agccgccaac
    48241 ggtgacatca attcccaggt cttttaatac tttggccacg ttgatgcctt tacccgccgc
    48301 atgcagaccg gtggttttca ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc
    48361 aacaaggtca taagccggat taagggtgat cgtagcaaca cgtctgctca ttatgcgccc
    48421 tccccaagac cagcagcgat agcgtcgcca atggctttca gcgcctgttc agcatctgca
    48481 ccctgggcgg taaagcgtag gcgatgacct ttcttaacgc caagtgccac aactttcatc
    48541 agactacgtc cgtttgccgg tttgccggtg ccatcaaggt ttgtcacggt aatatcactg
    48601 ttaaattgtt taatggtatt gaccagcatg gtacctggac gagcatgcag gccgtgttca
    48661 ttgcgcacca caaactccgc gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc
    48721 agcgccagca acgttgccgc atccgctttc agcaagcggt cagctttatt gtcgagcaat
    48781 aaatcagcga gacgcttaag aaccgcgatg ggctgatcgt cattcatcgc cacactcacc
    48841 agcatggctg ccgtttcgcc gtccacatca aaagcatttg ccgcacggct taccgcaatc
    48901 gcgctacgca gattgccttc ggcgctatcg ctcagccaga taccctgtcc gagattcagc
    48961 ggttgttcat tgatggcttt ggtgacgaaa gtggcgtcaa ctgcccccac ctctttcaga
    49021 cgcgcagcgt tcagcgcctg aagagtcagc agatcgctgg cgacgatatc cagcgtcagc
    49081 atttcgttgt cgagcttcag ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt
    49141 tcttctgctg ttgttgctga cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg
    49201 gtcagctggc gtagcaggcc cagatgttca tccgagctgg cagcaatacc gattgccacg
    49261 tacgctacct gaccgtcacc ccaggtgacg ccttccggga actgaaatac ctgaacgccg
    49321 gttttcagca cctgatcgcg ggtgtcggta gtgccgtgtg gaatagcaat accattgccg
    49381 aggaacgttg aggtttgctg ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca
    49441 ttaccggcct gcaccagcgc cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc
    49501 ttttcgcccg gatggatgtc ctgtacggat aactggaaca tagttctcct ctcttgctga
    49561 attgaaacga ttcagcctct atgagaaaaa aagcgccaac ctggcttagg gttaaagaca
    49621 agatcgcgct gaaacgtttc aagaaagcat aatacttctg tttcagcacg caaggaaagc
    49681 cgtaaagtta tgagcaaaag tttgatgtgc tgcacaattt tttgtcattt ttcctaattg
    49741 ctgatgggaa aatccgttat cagcaatttc atttcagcag cgtcagcagc caatcagcaa
    49801 cagtgaaatg gcattttgat tattcgtggc gcttttgatt tcgctgtctg tttattttct
    49861 gacaagcagc gtaaactccg cgtcttcctc ttccagtgat cgacctgcat gcataactcc
    49921 cccgcagtct ccagcgcgaa atcgtttgac ctgacctcga cggcgttttt aatcgttgcc
    49981 tttctcaccg gtattgcggg tgctctgcaa accccgacac tcagtatttt ccttaccgat
    50041 gaagtacatg cccgtccggc gatggtggga ttcttcttta ccggcagcgc tgtcattggg
    50101 attctggtaa gtcagtttct cgctgggcgc tctgataagc gcggcgatcg caaatcgctg
    50161 attgtctttt gctgcctgtt aggcgtgctg gcctgcaccc tttttgcctg gaatcgcaac
    50221 tactttgttt tgctattcgt tggcgtcttt cttagcagct ttggctcgac cgctaacccg
    50281 caaatgtttg cccttgcccg tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc
    50341 agctcttttt tacgcgctca ggtttcactg gcatgggtca ttggcccacc gctggcttat
    50401 gcgttagcga tgggtttcag ctttacggta atgtatctga gcgcagcggt agcgtttatt
    50461 gtttgcggcg tgatggtgtg gctgttttta ccgtcgatgc aaaaagagct tccgctggcg
    50521 accggcacgg ttgaagcgcc gcgccgtaac cgtcgcgata cgctactgct gtttgtcatt
    50581 tgtacattga tgtggggctc gaacagcctg tacatcatca acatgccgct atttattatc
    50641 aacgaactgc atcttcccga gaaactggcc gatgtgatga tggggaccgc cgccgggctg
    50701 gaaatcccga cgatgttgat tgccggatat ttcgccaaac gtctgggtaa gcgtttctta
    50761 atgcgcgttg ctgccgtggg tggcgtctgt ttttacgcag gaatgctgat ggcgcattca
    50821 cctgtcattc tgttgggctt gcagctgcta aatgctattt ttattggcat tctgggcggc
    50881 atcgggatgc tctattttca ggatctgatg cccggtcagg cgggttcagc caccacgctc
    50941 tataccaaca cttcgcgcgt gggctggatc atcgcaggat cagtggcggg catcgtcgcc
    51001 gaaatctgga attatcacgc tgtgttctgg tttgcgatgg tgatgattat cgccactctg
    51061 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
    51121 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
    51181 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
    51241 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
    51301 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
    51361 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
    51421 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
    51481 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
    51541 aacggcaaac tgctgctggt taaggatatt gatattcagt cgcccactgc ccgcggtgcc
    51601 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
    51661 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
    51721 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
    51781 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
    51841 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
    51901 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
    51961 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
    52021 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
    52081 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
    52141 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
    52201 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
    52261 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
    52321 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
    52381 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
    52441 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
    52501 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
    52561 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
    52621 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
    52681 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
    52741 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
    52801 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
    52861 aatggccatg tggtgaaaaa cgcggtgctg ggaatggcag aaaaatgttc gccagaactc
    52921 gccgggtgga taaaaaaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
    52981 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
    53041 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
    53101 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
    53161 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
    53221 ttcgtccata tcagtgattg catgcaggat cgcgtatttc gccatgctgc cagaacatta
    53281 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
    53341 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
    53401 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
    53461 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
    53521 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
    53581 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
    53641 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
    53701 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
    53761 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
    53821 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
    53881 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
    53941 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
    54001 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
    54061 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
    54121 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
    54181 gatctgttaa ccgcaccagt ctatgaactg tggatagatc tgcgcgccac cttgtgcatt
    54241 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
    54301 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
    54361 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
    54421 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
    54481 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
    54541 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
    54601 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
    54661 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
    54721 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
    54781 gagctgattt ccagcagcga agctgactgg aatgctttgc agagcgcgct gttgaagctt
    54841 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
    54901 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggcctcgcg ctgtttcttt
    54961 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
    55021 tctttaatca gaaactggtc gaaagtaagg cctttttgtg gctggttgca ttgaccaaca
    55081 atcgcgcttt cgacggttgt tctctgctgg cgatgggaat gttgatgctg agtttctggc
    55141 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
    55201 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
    55261 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
    55321 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
    55381 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
    55441 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
    55501 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
    55561 ccttttttga caaatcacta ccgggaaaaa acaaacattt ccaaaacaaa taactcacag
    55621 taattaacat catcaggctt atttttatag tgaggataat cctgatgatg cgcaccgtgc
    55681 tttcatccat cgcgcgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
    55741 ctcaatcgta ctttaattga ccaatcccgc ttattaactt tctgtatcac tttttcttat
    55801 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatcta gaactcgttt
    55861 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
    55921 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
    55981 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
    56041 cgattttgag atatatcttg cgcgggatcc ccgcgattgc agtagcggtt ctgctttctg
    56101 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
    56161 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
    56221 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
    56281 gcagcactaa aaaaggtatc gattgttctg gttttgtaca acgtacattc cgtgagcaat
    56341 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
    56401 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
    56461 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
    56521 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
    56581 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
    56641 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
    56701 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
    56761 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
    56821 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
    56881 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
    56941 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
    57001 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
    57061 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
    57121 tcaatgaatt gattccggcg ctcaacatta ataaaaacgt cgatatggcg atcttacccg
    57181 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
    57241 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
    57301 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
    57361 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
    57421 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
    57481 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctc tgctattacc
    57541 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
    57601 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgagtaacg ggcctggaag
    57661 tactgctacg ctggcggcat cctgtcgcgg gagaaattcc cccggatgcc ttcattaact
    57721 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
    57781 gcgatgccgc agaattagaa aaagtgctgc cagtaggcgt caaatttggt attaacattg
    57841 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
    57901 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
    57961 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
    58021 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
    58081 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
    58141 acgcggtgct gacgctggcg aaacgcctca atatgctgac ggttgctgaa ggggtcgaaa
    58201 cgccggaaca ggcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
    58261 ttagtcgccc tttaccgctg gacgattttg tccgctggct gaagaaaccg tatacgccgc
    58321 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
    58381 aatgatgagt aaaaggaaat ccattgcaga tgattgtgcg catactgctg ctgtttatcg
    58441 ctctgttcac ctttggtgtg caggcgcagg ctatcaagga aagctatgcc tttgccgtgc
    58501 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
    58561 caaaaggtgg gcagataacg ttgtcagccc tcggcacctt cgataatttc aaccgctatg
    58621 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgcta tttacgactt
    58681 ccgatgacga accaggcagt tattacccgc tgattgctga aagcgcacgc tatgctgacg
    58741 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttcgccca
    58801 ttactgcccg cgatgtagag tttacttttc aaaaatttat gaccgaaggc gtgccgcaat
    58861 ttcgtctggt ctacaaaggc accaccgtca aagccattgc accgttaacc gtgcgcattg
    58921 agttagctaa acccagcaaa gaagatatgc tgagtctgtt ttcgctgccg atatttccag
    58981 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
    59041 gtccgtaccg cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
    59101 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
    59161 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
    59221 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
    59281 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
    59341 cgtttaatat ccaacgtccg gtattcagcg atcgccgggt ccgggaagca atcactctcg
    59401 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
    59461 gttattttca gaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
    59521 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
    59581 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
    59641 acgaaacggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
    59701 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
    59761 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
    59821 ctaaccgcat gcgcagtcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
    59881 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
    59941 cccccggcgt gcaaagcccg gttatcgact cgctgatcaa ccaaattatt gccgcgcagg
    60001 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
    60061 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
    60121 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
    60181 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
    60241 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
    60301 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
    60361 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
    60421 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagac ccagaagtga
    60481 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
    60541 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
    60601 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
    60661 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
    60721 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
    60781 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
    60841 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
    60901 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
    60961 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
    61021 aaggggtaag tgagaaaaac attctctgga aacatgtgtt ccgcaacgcc atgctgctgg
    61081 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    61141 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    61201 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    61261 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    61321 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    61381 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    61441 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    61501 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    61561 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    61621 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    61681 ctggctggga acggatgcca acggcggcga tgtgctggca cgcattctct atggcacgcg
    61741 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    61801 gggggcgcta caaggctatt acggcggtaa agtcgatctc tggggacaac gctttattga
    61861 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    61921 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    61981 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    62041 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    62101 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    62161 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    62221 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    62281 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    62341 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    62401 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    62461 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    62521 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    62581 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    62641 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    62701 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    62761 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    62821 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    62881 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    62941 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    63001 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    63061 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    63121 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    63181 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    63241 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    63301 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    63361 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    63421 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    63481 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    63541 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    63601 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    63661 cattaattct taagccctca ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    63721 cggtacaggc gcaaatattg acgctattga aatcattgca acaaaagcat caactggcct
    63781 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    63841 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc gcaccgcagc
    63901 aggagtatac gcgtcagcta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    63961 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    64021 tggcgattta caaacaggca cggctcacct tcacactcca gcaccgaaca tggaacttcg
    64081 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    64141 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    64201 tcaaccttcg aacctgcgaa accagcagac cagcgaaagt tctgcggtag acgatggacg
    64261 attgagagtt gtaatgaagt cacttatttt ccccggaagc acatttactt cacaatttat
    64321 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    64381 cctcgcctgc atggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    64441 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    64501 gactttttac ataaattgat tttacataaa ataaacatat atcgggggaa tgatagattt
    64561 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
    64621 gttttttcgg tcgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    64681 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    64741 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    64801 gaaactcatc aagaatgacc gccatcgcat tggatgacac catagacacg cagcccacaa
    64861 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
    64921 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    64981 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    65041 caaagtaata accaaagttt tccggtgcaa cgtggttgat ttcaatgtag acaaacggtc
    65101 cggcgctcag gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca
    65161 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
    65221 gctggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
    65281 tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
    65341 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
    65401 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
    65461 acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg gcttacgccc
    65521 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
    65581 actcagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa ttaccggtag
    65641 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
    65701 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
    65761 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
    65821 atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca catggttacc
    65881 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
    65941 ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg agatcttttt cgttatgcag
    66001 ctgcacgcct ttagcaaatt gttctgccgt atcgtcagct acgggtgata ccagcgtcac
    66061 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
    66121 atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg cgtgcagttt
    66181 ccacgctacc ggttcatcta gaaaatagag caccgttggg tgatcagggt cgtccgtgga
    66241 gcaaacataa ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
    66301 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacggac
    66361 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
    66421 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
    66481 aaacggaggt cgggtaatga cccgaatatc tgaactgttg tctgcccagt atagcggtct
    66541 aataacatcc ctcaagggaa aaagatccgt ggcatactat tagcagaata atctacctac
    66601 gcgagaccat gatttttaca cttcgcccat atcagcaaga agccgtggat gccacgctca
    66661 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
    66721 gcctggtgat agcggaactg gcacggctgg ctcgtggtcg cgtactggtg ctggcacacg
    66781 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
    66841 tttttgccgc cgggctgaag cgtaaagaga gccacggtaa agtggtattt ggcagtgtgc
    66901 agtcggtcgc ccgtaatatt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
    66961 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
    67021 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
    67081 aaggttggat ctaccagttc cattatcacg gcatggtacg cagcgatgag aaagcccttt
    67141 tccgtgactg catttatgag ctgcccctgc gttatatgat taaacacagc tatctgacgc
    67201 cgccagaacg actggatatg ccagtagtgc aatatgattt cagccgcttg caggcacaga
    67261 gcaacgggct gttcagcgaa gccgatctca accgtgagct gaaaaaacag caacgtatta
    67321 ccccgcacat catcagccag attatggagt tcgccgaaaa gcgtaaagga gtgatgattt
    67381 tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgcag
    67441 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa aattttaaag
    67501 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
    67561 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
    67621 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
    67681 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
    67741 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
    67801 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
    67861 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
    67921 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
    67981 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
    68041 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
    68101 atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacacctgcc cagcgtaccg
    68161 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatcccg ctgcgctgga
    68221 tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acccccggat tttgtcgtcg
    68281 cccgcatgaa aggccagtac tggcaggtgc gtgaaaaagt gttcgattac gaaggtcgtt
    68341 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
    68401 agtatagaat ctcacccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
    68461 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
    68521 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
    68581 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
    68641 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
    68701 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
    68761 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccc gcggggtttt
    68821 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
    68881 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
    68941 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcagggtat cggtcgccgg
    69001 ctcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
    69061 gctaccggca aacttcgtca actgacgcag tgtgctccgg tctgccggga agctctcttc
    69121 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
    69181 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
    69241 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
    69301 gaaatcatca accgcttgca acagcccacg gttttgtgct ttcgtgttca atccttcgct
    69361 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
    69421 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
    69481 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
    69541 acgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg
    69601 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
    69661 caggcgacca gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
    69721 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
    69781 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
    69841 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
    69901 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
    69961 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
    70021 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
    70081 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
    70141 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
    70201 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
    70261 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
    70321 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
    70381 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
    70441 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
    70501 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact
    70561 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
    70621 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
    70681 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
    70741 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
    70801 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
    70861 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
    70921 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
    70981 acccgatgac ggcgcgacgt tttcttgaga agcatggtct gcttgatgcg caggagtatc
    71041 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
    71101 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
    71161 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
    71221 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
    71281 tctatggtat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
    71341 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
    71401 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
    71461 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccactac gcacaagaag
    71521 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
    71581 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
    71641 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggttgtgatt atcactgccg
    71701 gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac ggtcatctgc
    71761 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gcgctgactg
    71821 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
    71881 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
    71941 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
    72001 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
    72061 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
    72121 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
    72181 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
    72241 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccttt acggttggtt
    72301 tttttatatc tgcaattaat ttgataaaca gaccgtgaca catcacagcc tgtttatttt
    72361 ctgttatcag aacgtccaga ccactcccgc ctgagtattc cacggcgatt ctacccctgc
    72421 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
    72481 cccagcctga taaacgccac gggttccagc cacatcatta ttaaatttgc catcgtcatt
    72541 aactttcaca tggttagaat cggcgtattc ctgacgcacg gcggctttca gccagggttc
    72601 caacgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
    72661 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
    72721 atctgtggta aagccggtaa aggcgagata gggtctaaca ctccacaatc cgtcaaccca
    72781 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
    72841 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
    72901 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tgtagctatc
    72961 aatattgcct ttgccgccgc gatcaaaacc aatatcagaa tgagagtaat caaagaacaa
    73021 gccgcgaatt gtgctgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
    73081 cgtcaatgtt tgctcaaagc cagctcccgc atcagtggtc acgttgttgc gggtgttaat
    73141 tgccgaactc cacatcgccg tatcgtaatt aacgcctttt acgctaccaa gacgctcacg
    73201 cacggtgtcc agttctgcat caaataccag cggttgtgct gctgccatgt tcagcacatc
    73261 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
    73321 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
    73381 gaatgcagca tcaccgccgc ccgtcgttac cagtgtaagg ctatctcctg ctgccgggct
    73441 ggcccccgtg tccgtcacga atattttgaa atcacctgtt gcctgaccga tgacgttgag
    73501 ctgatcgctc tgatgattag ccatatcggt acgcatataa aattgccgtt tcccgacagg
    73561 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    73621 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    73681 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    73741 acgtccgctt ggctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    73801 actaccttac ccaccaacat agcgggtgca taatttattt tcggcttcat gacctcaaga
    73861 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    73921 ccattgttga taaccatatt ttcagagatg gctttagagg aatacgtgat acttccattc
    73981 tgatctgatc gaccgagatc atatacagca tttgcctgca ctttcgtacc agtatccttt
    74041 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg agtgtcctcc
    74101 ataacaatga gcccggagcc atcatccagt tcataatttt ttgacacacc atcctttata
    74161 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    74221 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaat
    74281 cctccctttt cgatagcggc aaagacggca atcccatctt tttcaacaat taagcgcccg
    74341 cctttaatcg tgggatcatt cgccgc
//
LOCUS       DAJVXP010000005        68401 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVXP010000005 DAJVXP010000000
VERSION     DAJVXP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.005"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..674)
                     /locus_tag="OQS10_000335"
     CDS             complement(<1..674)
                     /locus_tag="OQS10_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCS2291468.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OQS10_000336"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OQS10_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS2291469.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OQS10_000337"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OQS10_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS2291470.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OQS10_000338"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OQS10_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS2291471.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OQS10_000339"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OQS10_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS2291472.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OQS10_000340"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OQS10_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCS2291473.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="OQS10_000341"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="OQS10_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS2291474.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="OQS10_000342"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="OQS10_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS2291475.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="OQS10_000343"
     CDS             complement(8400..9752)
                     /gene="hyfD"
                     /locus_tag="OQS10_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS2291476.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9728..9838)
                     /locus_tag="OQS10_000344"
     CDS             complement(9728..9838)
                     /locus_tag="OQS10_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCS2291477.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="OQS10_000345"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="OQS10_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS2291478.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="OQS10_000346"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="OQS10_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCS2291479.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="OQS10_000347"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="OQS10_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS2291480.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="OQS10_000348"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="OQS10_000348"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS2291481.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="OQS10_000349"
     CDS             complement(14171..14743)
                     /locus_tag="OQS10_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS2291482.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="OQS10_000350"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="OQS10_000350"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS2291483.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="OQS10_000351"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="OQS10_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS2291484.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="OQS10_000352"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="OQS10_000352"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS2291485.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="OQS10_000353"
     CDS             17912..18760
                     /locus_tag="OQS10_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS2291486.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="OQS10_000354"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="OQS10_000354"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS2291487.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="OQS10_000355"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="OQS10_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS2291488.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="OQS10_000356"
     CDS             complement(21672..21872)
                     /locus_tag="OQS10_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS2291489.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="OQS10_000357"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="OQS10_000357"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS2291490.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="OQS10_000358"
     CDS             complement(23031..23387)
                     /locus_tag="OQS10_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS2291491.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23769..23828
                     /gene="ypfM"
                     /locus_tag="OQS10_000359"
     CDS             23769..23828
                     /gene="ypfM"
                     /locus_tag="OQS10_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS2291492.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="OQS10_000360"
     CDS             complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="OQS10_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS2291493.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="OQS10_000361"
     CDS             complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="OQS10_000361"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS2291494.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29110..31089
                     /gene="aegA"
                     /locus_tag="OQS10_000362"
     CDS             29110..31089
                     /gene="aegA"
                     /locus_tag="OQS10_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS2291495.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31157..31732
                     /gene="nudK"
                     /locus_tag="OQS10_000363"
     CDS             31157..31732
                     /gene="nudK"
                     /locus_tag="OQS10_000363"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS2291496.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31858..32901
                     /locus_tag="OQS10_000364"
     CDS             31858..32901
                     /locus_tag="OQS10_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS2291497.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="OQS10_000365"
     CDS             complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="OQS10_000365"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS2291498.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35020..35970)
                     /gene="tal"
                     /locus_tag="OQS10_000366"
     CDS             complement(35020..35970)
                     /gene="tal"
                     /locus_tag="OQS10_000366"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS2291499.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36259..38538
                     /gene="maeB"
                     /locus_tag="OQS10_000367"
     CDS             36259..38538
                     /gene="maeB"
                     /locus_tag="OQS10_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS2291500.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38831..39166
                     /gene="eutS"
                     /locus_tag="OQS10_000368"
     CDS             38831..39166
                     /gene="eutS"
                     /locus_tag="OQS10_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS2291501.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39179..39658
                     /gene="eutP"
                     /locus_tag="OQS10_000369"
     CDS             39179..39658
                     /gene="eutP"
                     /locus_tag="OQS10_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS2291502.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39633..40334
                     /gene="eutQ"
                     /locus_tag="OQS10_000370"
     CDS             39633..40334
                     /gene="eutQ"
                     /locus_tag="OQS10_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCS2291503.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40331..41134
                     /gene="eutT"
                     /locus_tag="OQS10_000371"
     CDS             40331..41134
                     /gene="eutT"
                     /locus_tag="OQS10_000371"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCS2291504.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41131..42147
                     /gene="pta"
                     /locus_tag="OQS10_000372"
     CDS             41131..42147
                     /gene="pta"
                     /locus_tag="OQS10_000372"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS2291505.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42186..42479
                     /gene="eutM"
                     /locus_tag="OQS10_000373"
     CDS             42186..42479
                     /gene="eutM"
                     /locus_tag="OQS10_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCS2291506.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42586..42873
                     /gene="eutN"
                     /locus_tag="OQS10_000374"
     CDS             42586..42873
                     /gene="eutN"
                     /locus_tag="OQS10_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCS2291507.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42885..44288
                     /locus_tag="OQS10_000375"
     CDS             42885..44288
                     /locus_tag="OQS10_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCS2291508.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44299..45135
                     /gene="eutJ"
                     /locus_tag="OQS10_000376"
     CDS             44299..45135
                     /gene="eutJ"
                     /locus_tag="OQS10_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCS2291509.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45125..46312
                     /gene="eutG"
                     /locus_tag="OQS10_000377"
     CDS             45125..46312
                     /gene="eutG"
                     /locus_tag="OQS10_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCS2291510.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46629..47855
                     /gene="eutH"
                     /locus_tag="OQS10_000378"
     CDS             46629..47855
                     /gene="eutH"
                     /locus_tag="OQS10_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCS2291511.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47852..49255
                     /gene="eutA"
                     /locus_tag="OQS10_000379"
     CDS             47852..49255
                     /gene="eutA"
                     /locus_tag="OQS10_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS2291512.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49267..50628
                     /gene="eutB"
                     /locus_tag="OQS10_000380"
                     /pseudo
     CDS             49267..50628
                     /gene="eutB"
                     /locus_tag="OQS10_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50649..51536
                     /gene="eutC"
                     /locus_tag="OQS10_000381"
     CDS             50649..51536
                     /gene="eutC"
                     /locus_tag="OQS10_000381"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCS2291513.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51546..52205
                     /gene="eutL"
                     /locus_tag="OQS10_000382"
     CDS             51546..52205
                     /gene="eutL"
                     /locus_tag="OQS10_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS2291514.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52218..52718
                     /gene="eutK"
                     /locus_tag="OQS10_000383"
     CDS             52218..52718
                     /gene="eutK"
                     /locus_tag="OQS10_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS2291515.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52764..53816
                     /gene="eutR"
                     /locus_tag="OQS10_000384"
     CDS             52764..53816
                     /gene="eutR"
                     /locus_tag="OQS10_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS2291516.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53822..54721)
                     /gene="hemF"
                     /locus_tag="OQS10_000385"
     CDS             complement(53822..54721)
                     /gene="hemF"
                     /locus_tag="OQS10_000385"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS2291517.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54725..55594)
                     /gene="amiA"
                     /locus_tag="OQS10_000386"
     CDS             complement(54725..55594)
                     /gene="amiA"
                     /locus_tag="OQS10_000386"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS2291518.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55808..56233
                     /locus_tag="OQS10_000387"
     CDS             55808..56233
                     /locus_tag="OQS10_000387"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS2291519.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56220..56669
                     /locus_tag="OQS10_000388"
     CDS             56220..56669
                     /locus_tag="OQS10_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS2291520.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56730..57305
                     /locus_tag="OQS10_000389"
     CDS             56730..57305
                     /locus_tag="OQS10_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS2291521.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57401..58300
                     /gene="yfeX"
                     /locus_tag="OQS10_000390"
     CDS             57401..58300
                     /gene="yfeX"
                     /locus_tag="OQS10_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS2291522.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58358..59662)
                     /gene="pbp4b"
                     /locus_tag="OQS10_000391"
     CDS             complement(58358..59662)
                     /gene="pbp4b"
                     /locus_tag="OQS10_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCS2291523.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59667..59840)
                     /locus_tag="OQS10_000392"
     CDS             complement(59667..59840)
                     /locus_tag="OQS10_000392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCS2291524.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59853..61091)
                     /gene="murP"
                     /locus_tag="OQS10_000393"
                     /pseudo
     CDS             complement(59853..61091)
                     /gene="murP"
                     /locus_tag="OQS10_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61095..61991)
                     /gene="murQ"
                     /locus_tag="OQS10_000394"
     CDS             complement(61095..61991)
                     /gene="murQ"
                     /locus_tag="OQS10_000394"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS2291525.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62155..63012
                     /gene="murR"
                     /locus_tag="OQS10_000395"
     CDS             62155..63012
                     /gene="murR"
                     /locus_tag="OQS10_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS2291526.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63141..63932
                     /gene="ucpA"
                     /locus_tag="OQS10_000396"
     CDS             63141..63932
                     /gene="ucpA"
                     /locus_tag="OQS10_000396"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS2291527.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64103..65119
                     /gene="cysP"
                     /locus_tag="OQS10_000397"
     CDS             64103..65119
                     /gene="cysP"
                     /locus_tag="OQS10_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS2291528.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65119..65952
                     /gene="cysT"
                     /locus_tag="OQS10_000398"
     CDS             65119..65952
                     /gene="cysT"
                     /locus_tag="OQS10_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS2291529.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65952..66827
                     /gene="cysW"
                     /locus_tag="OQS10_000399"
     CDS             65952..66827
                     /gene="cysW"
                     /locus_tag="OQS10_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS2291530.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66817..67914
                     /gene="cysA"
                     /locus_tag="OQS10_000400"
     CDS             66817..67914
                     /gene="cysA"
                     /locus_tag="OQS10_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS2291531.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68048..>68401
                     /locus_tag="OQS10_000401"
     CDS             68048..>68401
                     /locus_tag="OQS10_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HCS2291532.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQG"
BASE COUNT        16246 a        17906 c        18895 g        15354 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctagccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagttatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgctattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcgggtcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    23461 caggaaatat aacattatcg aagtattcaa attaacaact attaaataat attaaacatt
    23521 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa cctgaattaa
    23581 atcagtatta gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat
    23641 caccgcagga ccatagtatt tacataagat ttgtcccccc ggaggtagtc atcgcagcaa
    23701 tcgagtaaat tagcttcacc acggtatatt gtttccgtag aatacccata attataagag
    23761 aggttgttat gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc
    23821 gtaagtaatt cctgagacaa agtataaaca gcaagaaccc gctgagcagg ttcttaatcg
    23881 gaaagttgaa aaaggcgaca cattggcatg tcgccttttt tattgcttat tccgggcgcg
    23941 gctttagcgg gaagcggcgg cgcaccagca caaagaacag cggcacgaag taaatagcca
    24001 gaatagtggc tgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg
    24061 aaccggcacc cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca
    24121 tcagaatcgg gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc
    24181 ctttttggtt catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac
    24241 caatgaccgt taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg
    24301 ttgccagcag cgcgccgatt acccccagcg gcacgaccag cattaccgag aacggcaccg
    24361 accagctttc atacaacgca gccagacaca ggaataccac cagcaaggaa atggcgtaca
    24421 gcgccggagc ctgcgcgccg gaaagtcgct cctgatacga catcgccgtc cactccagac
    24481 caaagccgtt tggcagctgc ttcactaacg attccataat atccatcgcc gtaccggtac
    24541 tgacccccgg tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca
    24601 gacgcggcga gcctgtttcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc
    24661 catctttatt tcggacatac cagagattga tgtcatctgg cagcatgcga tacggcgcag
    24721 ctgcctgcac atagactttc ttcacgcgac cgcgatccat aaagtcattc acatagctcg
    24781 aaccccaggc ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag
    24841 ctttacgctg gtcgatatca atctgcaact gcggactgtc gtcgaggccg ttatggcgca
    24901 cacgggttag ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca
    24961 gcgcatcgtg acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac
    25021 caagaccgct aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa
    25081 acgcctttgt tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc
    25141 tccagtcttt caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag
    25201 aaccaacggt ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct
    25261 cgatttgctc aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg
    25321 aggtagtaaa catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca
    25381 ggaacaccat gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga
    25441 gaattttcgc cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc
    25501 aggcaaaaaa gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg
    25561 cacacagagc cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa
    25621 tggtaataga gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta
    25681 caaataccgc cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg
    25741 atttacgtgt ggcttcgcga ggagtgagtc cttcctcact cataatacgt tcgacgtttt
    25801 ccaccaccac gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg
    25861 ttaaggtgtt gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca
    25921 ccggcacggc gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa
    25981 cgaggaaaac cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt
    26041 taacaaacga ggtggtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg
    26101 ccagctcgtc gagacgattg agcaccagct ccgctgtcgc catttcgttt gcgccggagg
    26161 ccagttttac ccccagcccg gaggctggct taccattgaa gcggctaaga taatcgtatt
    26221 tctccgcccc catttcgacg gtggcgacat cgcccagcct tacctctgag ccgtcttgat
    26281 tcacccgcaa ggtgatatcg cggaactgtt ctggtgtttg cagcagtgac tgggcgttaa
    26341 tggtggcgtt gagcgcctgc ttatcgacgg aaggtgtacc accaagttgc ccaaccgcaa
    26401 tctgcgcgtt ctgtgactca atggcatcag tgacatcttt agccgtcatc tggaaactgt
    26461 tgagtttcgc cgggtccagc cagatacgca tggaatattg cgaaccatag gcatcgatat
    26521 ccccgacgcc attcacgcgg cttaacgggt cctgaatatt actggcaaca taatcggcaa
    26581 tatcctgttt atccatcgaa ccatcggtag agacgaaagc aatggtcaga atattggtat
    26641 cgccggtttt acgcaccgtc acgccctgat tttgcaccgc ctgcggtaac tttcgcatgg
    26701 ctgattgcag ctggttttgt acttgctgca cggcttcgtc cggatcggtg cctgctttaa
    26761 aacttaaagt gacagatgcc tgaccgatgc cactgctctg agatgacata tacatgagat
    26821 tatcgaggcc ggtcatattt tgctcgataa cctgggtcac ggtgttttcc agcgtctggg
    26881 ccgatgcgcc gggatagtta gcggtcactc gcacattcgg tggcgcgaga tcggggtatt
    26941 gttcaacggg caatgaaaaa atcgccaggg tccctgtcag acacaacagg attgccagca
    27001 cccaggcaaa aatggggcga tcaataaaga aattcgccat taaaagagga cctcgtgttt
    27061 ctgcgtatcg ttgtaggctt gacttgcttc actgtagcgg caaggtacga gccaatcgtg
    27121 gagaaaaaaa ggagttaatg taaattatca tgccctttta tactgaacct taagtgcaag
    27181 tattctttgg tcagtaggag gcattacatc aactgacttt cctcaccctc cgcagagcga
    27241 aagctaatac tcactaacgt gccgccgccg gaaggtggtg aaaaagtcag cgtcccaccc
    27301 agccgttccg cgcgttcgcg catgatattc agaccataat gaccttcggg ttctttcggt
    27361 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    27421 acgcaactga cggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    27481 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gcgccagggt tggcagacgg
    27541 cagtcgaggg tcagtttggc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    27601 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    27661 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    27721 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagtacc
    27781 tgagccagcg agtcgtgcaa ttcgcgggcg atggttgcac gttcttccat caacaataac
    27841 tgctgaaaat gcttttgcgc ctgattaaag tacaaaccgc gtcccagcat cgacgaaacg
    27901 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    27961 accgtctctt gcattgtcac cggtaaaatc tgcatcggca attccgggtt tggttgccct
    28021 tcgctaatcc gccagttttc accgacattt aactccagat attcagccgc ttcattgtcg
    28081 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggcttgt gttcagcgcc
    28141 tgcgaacatt gatacaacac ctccagacga cgcttggcct cgtggagatc gcgggtcttt
    28201 tcttctactg acgcctccag cgaaaggtac aatttatgca gctcgctcga catctggtta
    28261 aaggtttttg caagcagacc aagctcattc ggcagactgg tatccagcgg cggcgagtcg
    28321 aactgcccgt gttcaatacg ttgactggcg gtaaccagct gattcagcgg ggcaaccacc
    28381 tgatggcgta tacggcgcaa agtaaaaaag accagcgtga aaataccgat accgccagcc
    28441 agggaaatcg ccaccaccag cagcattttg cgttcagcgt agtgctgtaa agccagcacg
    28501 aacaggtcta tctgattaac ataattatta atattggcct gataccacgg caaatcgccc
    28561 ttgctgagcc gattattcat ctccagccag ttggcattca gatgcgcata gcgagttttt
    28621 actgcttctg gcacatacca gacgttgaga ttggttaata ccgatgaatg cagtgcctgc
    28681 tgaaacaact ggcgatgtgc attgagttgt ggactgccac tttgcaagtc gtagcccagg
    28741 cggtaactct gcatacgcag cgatccggca atattgatag cctcagcgtc gcgcaaactg
    28801 ctcgccagag ttagcagagc gatacccgtg gaaagaatcg acagcagcac aatgtaaaaa
    28861 aaggcccggg ccagactggc cgagacgggt cgtttaacaa tcacacgcgt cttctccaca
    28921 aaaaatcatt aatgttccag ttcgctaaaa cgcaatttag tcagtgatgg gattatttga
    28981 tctgccacag tttatagcga aatagttact tattctgggc acatgaacat tgccgattcc
    29041 cctgtaagtc gggtaataac aacactcata taaagaataa ggtttttaca accaaaaaag
    29101 aaggtcgtta tgaatcgttt tattatggcc aacagtcagc aatgtctggg ttgtcatgct
    29161 tgtgaaatcg cctgtgtcat ggctcacaat gatgagcaac atgtcctgag ccaacaccat
    29221 tttcatcccc gaattacggt tatcaaacat cagcagcaac gtagtgcagt gacctgtcac
    29281 cattgtgaag atgcgccctg cgcccgtagc tgccctaatg gcgcaatcag ccacgttgat
    29341 gacagcattc aggtcaatca gcaaaagtgt attggctgta aatcctgcgt ggtggcctgt
    29401 ccgtttggta cgatgcaaat cgtcctgaca cccgtcgcgg caggaaaagt aaaagccacg
    29461 gcgcataaat gcgacctttg tgcagggcgc gaaaacggtc ctgcctgtgt cgagaattgc
    29521 ccggcggacg cgctgcaact ggtcactgac gtcgcactct ccggcatggc gaaatcccgc
    29581 cgcttgcgca ccgcacgtca ggagcatcaa ccgtggcatg ccagtaccgc tgcacaagaa
    29641 atgccggtaa tgagtaaagt cgaacaaatg caggcaacgc ccgcgcgtgg cgagccggat
    29701 aaactggcga ttgaagcgcg caaaaccggt tttgatgaaa tttatctgcc attccgtgcc
    29761 gatcaggcac aacgggaagc ctcgcgctgc cttaaatgcg gcgagcacag cgtttgtgaa
    29821 tggacctgcc cgctgcataa ccatattccg cagtggattg aactggtgaa agccggaaac
    29881 atcgacgccg ccgtcgagct ttctcacctg accaacaccc tgccggaaat taccggacgc
    29941 gtttgtccgc aggaccgttt gtgtgaaggt gcctgtacta ttcgcgatga gcacggcgcg
    30001 gtaactatcg gcaacattga acgctacatt tcagatcagg cgttggcgaa aggttggcgt
    30061 cctgacttaa gccatgtcac caaagtggac aagcgggtgg cgattatcgg tgcgggtccg
    30121 gcagggctgg cctgtgcgga tgttctgacc cgcaatggcg tggcggtgac ggtgtacgat
    30181 cgccatccag aaattggtgg cttgctcact ttcggcattc cttctttcaa actggataaa
    30241 tccctgctgg cacgccgtcg ggaaatcttc agcgcgatgg ggattcactt cgaactcaat
    30301 tgtgaagtgg gtaaagatgt ctctttggat tcgcttttgg aacaatacga cgcggtcttc
    30361 gttggcgtag gcacttaccg ttccatgaaa gcgggtttac ccaatgaaga tgcgccgggc
    30421 gtttatgacg cgctgccgtt cctcatcgcc aacactaaac aggtgatggg actcgaagag
    30481 ctaccggaag agccgtttat caataccgcc ggactcaacg tcgtggtact gggcggcggc
    30541 gacaccgcga tggactgtgt gcgtaccgcg ctgcgccacg gcgcgagtaa cgtcacctgc
    30601 gcttatcgtc gtgatgaagc taacatgcca ggctcgaaga aagaggtgaa gaacgcccgc
    30661 gaagaggggg ccaacttcga atttaacgtc cagccggtgg cgcttgagct gaatgagcaa
    30721 ggtcacgttt gcggaattcg tttcctgcgc acgcgtcttg gcgagccgga tgcccagggg
    30781 cgccggcgac ttgtaccgat tgagggcagt gaatttgtca tgccagccga tgcggtgatt
    30841 atggtgtttg gcttcaatcc acacgggatg ccgtggctgg agtcgcacgg tgtaacggta
    30901 gacaaatggg gccgcatcat cgcggatgtg gaaagccagt accgttacca gaccaccaat
    30961 ccgaaaatct tcgccggtgg cgacgccgtg cgtggtgcgg atctggtggt taccgcaatg
    31021 gcagaaggac gtcatgcggc acaggggatt attgactggc tgggggtaaa atcagtcaaa
    31081 tctcactgat agcctgcgca gacaaacccg acttcacagc gtaatataat tgttcatttc
    31141 gcgctgtgga gtcggtatga cgcaacaaat caccctcatt aaagacaaaa ttctctccga
    31201 taactatttc accctgcaca acattactta cgatctcacg cgcaaagatg gcgaagttat
    31261 ccgccataaa cgtgaagttt acgatcgcgg taatggtgcg acgatcctcc tgtacaacgc
    31321 gaagaaaaag accgtggttc tgattcgtca gttccgtgtc gctacctggg ttaatggcaa
    31381 tgaaagcggg cagctgattg aaacctgcgc cgggctgctg gataacgacg aaccggaagt
    31441 gtgcattcgt aaagaagcga ttgaagagac gggctatgaa gtgggcgaag tgcgcaaatt
    31501 atttgaactg tatatgtcgc ctggcggtgt gactgagcta atccactttt ttatcgccga
    31561 atacagtgac aatcagcgcg ctaacgccgg tggcggtgtt gaagatgaag atattgaagt
    31621 gcttgagctg ccgttcagcc aggcgctgga gatgatcaaa accggcgaga tacgtgacgg
    31681 taagacggtg ttattgctta actatttgca aacgtcacat ttaatggact gaaaaataac
    31741 aataatattt cgttgtttat tattggctca atccgacaaa tctgattgag ccgcgctacc
    31801 tgcgcaacga agatacgact tgtgctgttt gtttgaactt ctggggtcgt accgtccatg
    31861 cgctatcgca ttttccttct cttttttttc gctttgttgc cgacgtcttt ggtgtgggcg
    31921 gcactagcgc aacgggcgtt ttccgactgg caggtcacct gtaataacca aaacttctgc
    31981 gtggcgcgca atacgggcga tcataatgga ctggtgatga ccctgagccg cagcgccggg
    32041 gcgcataccg atgccgtttt acgtattgag cgcggcgggt tgaagtcgcc ggatgcgtct
    32101 gaaggagaga tagcgccacg gctgctgtta gatggtgagc cgttggcgtt aagtggtgac
    32161 aagtggcgga tttcaccatg gttattagta accgatgata cggcaaccat caccgcgttt
    32221 ttgcagatga ttcaggaagg gaaggcgatc accttacgcg acggcaatca gaccatttct
    32281 ctgagtggct taaaagcggc gttgttgttt attgatgctc agcaaaagcg cgttggcagt
    32341 gaaaccgcgt ggatcaagaa aggggacgaa ccgccgctca gcgtaccgcc cgcgcctgcg
    32401 ctgaaagagg tcgcggtggt taacccaacg ccgacgccac tctcactcga agaacgcaac
    32461 gatttgctgg attatggaaa ctggcggatg aatggtctgc gctgctcgct tgatccgtta
    32521 cgtcgtgagg tgaatgtcac tgcgctgact gatgataaag cgctgatgat gatcagctgt
    32581 gaggcagggg cctataacac cattgatttg gcatggattg tgtcgcgtaa aaagccgcta
    32641 gcctcgcgcc cggttcggtt gcgtttgccg ttcaacaacg ggcaggagac gaatgaactg
    32701 gaactgatga acgcaacatt tgatgagaaa tcgcgtgaac tggtgacctt agcgaaaggg
    32761 cgcggattaa gcgattgtgg cattcaggcg cgctggcgct ttgacggtca gcgttttcgc
    32821 ctggtgcgtt acgccgcaga acccacctgc gataactggc atgggccaga cgcctggccc
    32881 acgttgtgga tcacccgtta aacaaaatgg cggggacatg taggccggat aagacgctgt
    32941 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcactatcag
    33001 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    33061 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    33121 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    33181 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    33241 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcctgc taatttctcc
    33301 gcagcttgca gggtgatttc catctctgaa ccggtggcaa tcagaataat atctggctta
    33361 ccgccgctgt ctttcagcac ataaccgcca cgagtaatct ctttaacctg atccggcgta
    33421 cgttccacct gggccagatt ctgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    33481 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    33541 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    33601 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    33661 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtcgc ggtatacggc
    33721 acaaagccgc cgtggtgcgc gatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    33781 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    33841 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag caaggggcca
    33901 taagcgttaa gcgtattttg cgaagcctta cgggtggcga ttttcgccgg attcgcctgc
    33961 aactcattga tatacttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    34021 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    34081 ttccacctct gctgcgcttt ttcgcctttt tcgcgggcat cccaggcgtg ataaatatct
    34141 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtgccag cgccacttct
    34201 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgatccaaag
    34261 ccaatcaccg tacgacagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    34321 atcgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    34381 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttctgtttc accgtcgatg
    34441 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcctgccagc
    34501 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    34561 aagtgatcga cgatctcatg gtctggctgg ttaaactgcg ccgccagtgt acgctctgct
    34621 atcgccagcc cgacggcgtt cgccaaacct tgtccaagtg gaccggtggt ggtttcaacg
    34681 cctggcgtat aaccaatctc cgggtggcct ggggttttcg aatgcagttg acgaaagttc
    34741 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    34801 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    34861 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    34921 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    34981 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    35041 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    35101 cgccatcgca tactgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    35161 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    35221 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    35281 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    35341 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat acgggtccat
    35401 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    35461 gcctgcttcc gcacaggccc gtgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    35521 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagctt
    35581 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    35641 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    35701 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    35761 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    35821 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    35881 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    35941 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    36001 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    36061 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    36121 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    36181 ataatgtgcg ccacgttgtg ggcagggggt gttgcccaca cactttattt gtgaacgtta
    36241 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    36301 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    36361 ctggcgctgg cctactcacc cggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    36421 ttaaaagcct acaaatatac cgcccgcggt aacctggtgg cggtgatctc taacggtacg
    36481 gcggtgctgg ggttaggcaa cattggcgcg ctggctggca aaccggtgat ggaaggcaag
    36541 ggcgttctgt ttaagaaatt cgccgggatt gatgtatttg acattgaagt tgatgaactc
    36601 gacccggaca aatttattga agttgtcgct gcgctcgaac cgaccttcgg cggaatcaac
    36661 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    36721 aatattccgg tattccacga cgatcagcac ggcacggcaa tcatcagcac tgccgccatc
    36781 ctcaacggct tgcgcgtggt ggagaaaaac atctccgacg tgcggatggt ggtttccggc
    36841 gcgggtgccg cagcaatcgc ctgtatgaat ctgctggtag cgctgggtct gcaaaaacat
    36901 aacatcgtgg tttgcgattc aaaaggcgtt atctatcaag gccgtgagcc aaacatggcg
    36961 gaaaccaaag ccgcatatgc ggtggtggat gacggcaaac gtaccctcga tgatgtgatt
    37021 gaaggtgcgg atattttcct gggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    37081 aagaaaatgg ctcgggcacc aatgattctg gcgctggcga atccggaacc ggaaattctg
    37141 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gcaccggtcg ttctgactat
    37201 ccaaaccagg tcaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    37261 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    37321 gcccatgcgg aacagagcga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    37381 ggtccggaat acatcattcc aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct
    37441 gcggtcgcga aagcggcgat ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac
    37501 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    37561 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    37621 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    37681 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    37741 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    37801 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    37861 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    37921 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    37981 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    38041 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    38101 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    38161 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    38221 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcggcgctg
    38281 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    38341 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    38401 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    38461 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    38521 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    38581 gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata ataaaaaaag
    38641 cagtctatat aatatctcga tattatttta tttatattca tgcgttgcat atgaaagttt
    38701 atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt aaacgcgctt
    38761 gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag tttttcacag
    38821 gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca aacaggtcac
    38881 actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg gcgttccgga
    38941 tgcgggcgta attggcatta tgacgctaac tcccggcgaa acggcgatga tcgtcggtga
    39001 tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca gcggtgcatt
    39061 ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt
    39121 aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg aggcattcat
    39181 gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat ttaatgcgtt
    39241 gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg ataagggcga
    39301 tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct taattaccac
    39361 gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg aaagtcggtt
    39421 gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac
    39481 ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga
    39541 agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac tggtggatta
    39601 tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca gcgaatgata
    39661 ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc gccagcatta
    39721 ttaccccgga agcccgtaaa gtggcggatc tactgggttt taccatcacc gaatgtgatg
    39781 aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc
    39841 agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc gaaagcctgg
    39901 tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag ggcgcaatgc
    39961 agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat ggcagcagcg
    40021 tcaagtttgg tcgttttgat ggcgcacaac cgcattgcgt gggcttgact gatcttgtga
    40081 ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac gcattcttcc
    40141 cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagctc catgttcgcc
    40201 acgaaggcga aaccatgatc gccaaagcag gcgacgtgat gtttatcccg aaaggctcca
    40261 gcatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg ccggctaact
    40321 ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc atacgctcag
    40381 cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg cccgggagtt
    40441 gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc tgtttgttga
    40501 cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg aacatccgca
    40561 ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc tgactcacct
    40621 gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg cggttctcga
    40681 cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt ggcagccgtg
    40741 gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc tgggggaacc
    40801 gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcgac tttctcacca
    40861 gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg ggcgcgatgc
    40921 ggcgttgctc aatctgctac gtaccaaagt gcgggaaacc gaaaccgtag cggcgcaggt
    40981 gtttatcacc cgcagctttg aagtattacg cccggacatc ttgcaggcgc tgaaccgcct
    41041 ttcgagcacg gtctacgtaa tgatgatcct gagcgtaacg aagccgccgc taacggtgaa
    41101 gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg tgaactggcg
    41161 ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag atcaacgtgt gctgaaagct
    41221 gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgctaa tccgtttgaa
    41281 cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt gattgatccg
    41341 catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg cgcgggcgaa
    41401 aaaacgccgc cggatgcgct ggaaaaactt accgatccgc tgatgttcgc cgcagcaatg
    41461 gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac ggcgaatgtg
    41521 ctgcgtgccg gattacgtat tatcggcttg cagccaggct gtaaaacgct ctcatccatt
    41581 ttcctgatgc tgccacagta cagcggtccg gcgttgggct ttgccgattg cagcgtggtg
    41641 ccacagccga cggcggcgca actggcggat atcgcgcttg ccagtgccga aacctggcgc
    41701 gccctcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagtaa cggtagcgcc
    41761 cgtcacccct gcgttgccaa cgtccagcag gcgacagaaa tcgtccgtga gcgcgcacca
    41821 aagctggtgg ttgatggcga gttgcagttt gacgccgcct tcgtgccgga agtggcggcg
    41881 caaaaagcgc ctgccagccc gctacagggc aaagccaatg tgatggtttt tccgtcgctg
    41941 gaagccggaa atattggcta caaaatcgca caacgactcg gcggatatcg tgccgtcggg
    42001 ccattgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg tagcgtgcag
    42061 gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacagaagt gaaccgcgaa
    42121 agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat aaagaggaaa
    42181 gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg attgaggctt
    42241 ctgacgcgat ggttaaagct gcgcgcgtca agctggttgg tgttaagcag attggcggcg
    42301 gcctgtgtac tgccatggtt cgtggtgatg ttgcagcctg taaagccgcg accgatgcag
    42361 gtgccgcagc ggcacagcgt atcggcgagc tggtatctgt acacgttatc ccgcgtccgc
    42421 acggcgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc aacctgtaat
    42481 caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt accttctgcg
    42541 gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa actggcagtc
    42601 gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga caaattgctg
    42661 atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt cgccatcgac
    42721 aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc cgcccgccag
    42781 gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt cgatgaggtg
    42841 gtgtctggcg gtcaggtaat tttccacaaa taaggcagaa catcatgaat caacaggata
    42901 ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg ccgcccgccg
    42961 ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca gccaaagtcg
    43021 cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc attcgtgagg
    43081 caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc ggcatgggcc
    43141 gcgttgaaga taaatttgcc aaaaacgtcg ctcaggcgcg cggcacgcca ggcgttgagt
    43201 gtctctctcc gcaggtgctg accggcgaca acggcctgac tttaattgaa aacgcgccct
    43261 ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta attaataacg
    43321 ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg gcggcgaaaa
    43381 aagtctccca gcgggcgatc acgctgctca accaggcgat tgttgccgca ggcgggccgg
    43441 aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcagcgt ttgttcaagt
    43501 ttccgggcat cggccttctg gtggtgaccg gcggcgaagc ggtagtagaa gcggcgcgta
    43561 aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta gtggtggatg
    43621 aaaccgccga cctcgcccgt gccgctcagt ccatcgtcaa aggcgcttct ttcgataaca
    43681 acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc gatgaactga
    43741 tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg cagcagctgc
    43801 aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc cgtgactggg
    43861 ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg caagaaacgc
    43921 gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa ctgatgatgc
    43981 cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg gcggtgaaac
    44041 tggaaggcgg ttgccaccac acggcggcaa tgcactcgcg caacatcgaa aacatgaacc
    44101 agatggcgaa tgctattgat accagcattt tcgttaagaa cggaccgcgc attgccgggc
    44161 tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc ggtgaagggg
    44221 taactagcgt gcgtacgttt gtccgtctgc gtcgctgtgt attagtcgat gcgtttcgca
    44281 ttgtttaagg agtaacagat ggcgcacgac gaacaatggc tcaccccacg tctgcaaacg
    44341 gcggcaacgc tgtgtaacca gacgcccgcc gcgacggaat ctccgctgtg gctgggggtg
    44401 gatctgggca cctgcgatgt ggtgtcgatg gttgtcgacc gcgacggtca gtcggtggcg
    44461 gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt cttcggcgct
    44521 gtcaccattg ttcgtcgcca tctcgacacg ctcgagcagc aattcggtcg ccgttttagc
    44581 catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa cgtgctggag
    44641 tctgctggac tggaagtgag ccatgtgctg gatgagccaa ccgcagttgc ggatctgctg
    44701 caactggaca acgccggtgt agtggatatc ggcggcggca ctaccggcat tgccatcgtg
    44761 aaaaagggca aggtgacgta ctcggcggat gaagcaaccg gcggacatca catctctctg
    44821 actctcgccg gaaatcgccg tatttcgctg gaagaggcgg agcagtacaa gcgcggtcac
    44881 ggtgaagaga tttggcctac ggtgaaaccg gtgtacgaaa aaatggcgga cattgtcgcc
    44941 cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc ttgtatgcaa
    45001 ccgggcgtgg cggaactgtt tcgcaaacaa ttcccggcgt tacaggtgca tttaccgcag
    45061 cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa agcggagggg
    45121 ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc tgaatctgca
    45181 acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtccgggct cggtgagcag
    45241 ttgcggacag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg cagacagctt
    45301 tttgcatcag gcagggatga ccgccgggct gacgcgcagc ctggctgtta aaggcatcgc
    45361 catgacgctc tggccatgtc cggtgggcga accgtgcatc accgacgtgt gtgcagccgt
    45421 ggcgcagttg cgtgagtcag gctgtgatgg ggtgatcgca tttggcggcg gctcggtgct
    45481 ggatgcggcg aaagccgtgg cgttgctggt gacgaacccg gatagcacgc tggcagagat
    45541 gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa cgaccgccgg
    45601 aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg ggcgcaagca
    45661 ggtgttagcc catgcctcgc tgatgccgga tgtggcgatt ctcgacgccg cgttgaccga
    45721 aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc atgccattga
    45781 agcatacagc gccctgaacg ctacaccgtt taccgacagt ctggcgattg gtgccattgc
    45841 gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg ccgcgcgcga
    45901 gagcatgttg ctggcttcat gtatggcggg aatggcgttt tccagtgcgg gtcttgggtt
    45961 gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccacacg gtctggcgaa
    46021 cgccatgttg ctgccaacgg tgatggaatt taaccggatg gtttgtcgtg aacgctttag
    46081 tcagattggt cgggcactgc gaactaaaaa atccgacgat cgtgacgcta ttaacgcggt
    46141 aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg gtgcgacatc
    46201 tgcgcattac ggcgcatggg cgcaggccgc gctggaagat atttgtctgc gcagtaaccc
    46261 gcgtaccgcc agcctggaac agattgtcgg actgtacgcg gcggcgcaat aaatgccgga
    46321 tgcgacgctt gccgcgtctt atccagccta cgggattgca catgtagggc ggataaggcg
    46381 ttcacgccgc atccgccagt ggcgcggtgc aattgccgga tgcgatgctt gccgcgtctt
    46441 atccggccta cgggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagt
    46501 ggcgcggtgc aattgccgga tgcgacgctt gccgcgtctg atccggcctt cgggattgca
    46561 catgtagggc ggataaggcg tttacgccgc atccgccaat aaataacaac taacagggag
    46621 taaaggcgat gggaattaac gaaatcatca tgtacatcat gatgttcttt atgctgatag
    46681 ctgccgtaga caggatcctg tcgcagttcg gcggttctgc tcgtttcctc ggtaagttcg
    46741 gtaaaagtat cgaaggatca ggcggtcagt tcgaagaagg ctttatggca atgggcgctc
    46801 tgggtctggc gatggtcggt atgaccgcgc tggcaccggt actggctcac gtactcgggc
    46861 cggtaattat cccggtttac gaaatgctcg gcgcaaaccc gtcaatgttc gccggaacgc
    46921 tgctggcgtg cgatatgggc ggcttcttcc tcgccaaaga gctggcgggc ggcgacgtag
    46981 cagcgtggct atactctggg ttaattcttg ggtcgatgat ggggccaacg attgtgtttt
    47041 ccattccggt ggcgctcggc attatcgaac cttctgaccg tcgttatctg gcgctcggcg
    47101 tgctggcggg cattgtgacc attccgattg gctgtattgc tggtggtctg gttgctatgt
    47161 actccggtgt gcagatcaac ggtcagccgg tggaattcac tttcgccctg atcctgatga
    47221 acatgatccc ggtgatcatt gttgcgattc tggtggcgct ggggctgaaa ttcatcccgg
    47281 aaaaaatgat caacggcttc cagatcttcg ccaaattcct cgttgcattg atcaccctcg
    47341 gtcttgctgc cgcggtagtt aagtttctgc ttggctggga actgatcccc ggtctggacc
    47401 ctatctttat ggcccctggc gataaacccg gtgaagtgat gcgcgccatt gaagttatcg
    47461 gttctatctc ctgcgttctg ttaggggcgt atccaatggt gctgctgctg acccgctggt
    47521 ttgaaaaacc gctgatgagc gtcggtaaag tgctgaatat gaacaacatc gcggcagccg
    47581 gcatggtggc aacgcttgcc aacaacatcc cgatgttcgg catgatgaag cagatggata
    47641 cccgcggcaa agtcatcaac tgcgccttcg ccgtttccgc tgctttcgcc ctgggcgacc
    47701 acttaggctt cgccgctgcc aacatgaacg ccatgatctt cccgatgatt gtcggcaagt
    47761 tgatcggcgg cgtaacggcg attggcgtgg cgatgatgct ggtgccaaaa gaagacgcga
    47821 ccacggctaa aaccgaagcg gaggcacaat cgtgaacact cgccagctat tgagcgtcgg
    47881 tatcgatatc ggcaccacca ccacccaggt gattttctcc cacctggagc tggttaaccg
    47941 tgcggcggtg tcgcaggtgc cgcgctacga attcattaaa cgcgaaatta gctggcaaag
    48001 cccggtgttc tttacccctg tcgataaaca gggtggttta aaagaagcgg aactgaaaac
    48061 cttaatcctc gagcaatatc aggctgcggg cattgcgccg gaaagcgttg attctggtgc
    48121 catcatcatc accggtgaaa gcgcgaaaac ccgcaatgct cgtccggcgg tgatggtgct
    48181 ctcccagtcg ctgggcgatt ttgtcgttgc cagcgccggg ccgcacctcg aatccgtcat
    48241 tgccggtcac ggtgccgggg cacaaaccct ttctgaacaa cggctgtgtc gggtactgaa
    48301 tattgacatc ggcggtggca ctgcgaacta cgccctgttc gatgccggaa aaatcagcgg
    48361 cactgcctgc ctcaacgtcg gtggtcgcct gctggaaacc gacagccagg ggcgcgtggt
    48421 ttacgctcat aaaccggggc agatgattgt ggatgagtgc ttcggcgcag gcaccgacgc
    48481 ccgttcgctg accggcgcgc agctggtgca ggttacccgg cggatggcag aactgattgt
    48541 cgaagtgatt gacggaacgc tctcgccgct cgcgcaggca ttgatgcaaa ccggtttgct
    48601 gcccgcaggt gttacgcccg aaatcattac gctttctggc ggcgtgggcg aatgttatcg
    48661 ccaccagccc gccgacccgt tctgttttgc cgatattggc ccgctgctgg caacggcgct
    48721 gcatgaccat ccgcgcctgc gtgagatgaa cgtacagttt ccggcgcaaa ccgtgcgcgc
    48781 cacggtgatt ggcgcgggcg cgcataccct ttcgctctct ggcagcacaa tctggcttga
    48841 gggcgtacaa ctgccgctgc gcaatttgcc ggtggcgatc ccgattgatg aaacggatct
    48901 ggtgagtgcc tggcaacagg cgctgcttca gctggatctt gatcccaaaa ctgacgcgta
    48961 cgtgctggcg cttcccgcct cgctgcctgt gcgttacgcc gcggtactga cggtcatcaa
    49021 cgcgctggtc gatttcgtcg cgcgttttcc aaatccgcat cccctgctgg tggtggccgg
    49081 gcaggacttt ggtaaagctc tgggcatgtt gttgcgccca cagctacaac aactcccgtt
    49141 ggcagtcatt gacgaagtga ttgtccgcgc gggggactat atcgacattg gtacgcctct
    49201 ttttggcgga tcggttgtgc cggtgacggt gaaatcactc gcatttcctt cctgagggaa
    49261 cgacttatga aactaaagac cacattgttc ggcaatgtat atcagtttaa ggatgtaaaa
    49321 gaggtgctgg ctaaagccaa cgaactgcgt tcgggggatg tgctggcggg cgttgcagcg
    49381 gcaagctcac aggagcgcgt ggcggcaaag caggtgttgt cggaaatgac cgtagcggac
    49441 atccgcaata atccggtgat tgcctatgaa gatgactgcg tgacgcggct gattcaggac
    49501 gatgttaacg aaacggccta caaccagatt aaaaacagga gcatcagcga actgcgtgag
    49561 tatgtgctga gcgatgaaac cagcgtggac gacattgcct ttacccgcaa agggctgacc
    49621 tcggaagtgg tcgcggcggt agcgaagatt tgctccaacg cggacctgat ctacggcgcg
    49681 aagaaaatgc cggtaatcaa aaaggccaat accaccatcg gtattccggg caccttcagc
    49741 gcccgtttgc agccaaatga cacccgtgac gacgtgcaaa gtatcgctgc acaaatctac
    49801 gaagggcttt ccttcggggt gggcgatgcg gtgatcggcg ttaacccggt aactgacgac
    49861 gtggaaaact taagccgcgt gttggacacc atttacggcg tgatcgacaa attcaacatc
    49921 ccaactcagg gctgcgtact ggcgcacgtc accacccaga tcgaagcgat tcgtcgcggc
    49981 gcgcctggcg gactgatttt ccagagtatc tgtggcagcg aaaaagggct gaaagagttt
    50041 ggcgtggaac tggcgatgct cgacgaagcg cgcgcagtgg gtgctgagtt caaccgtatc
    50101 gccggggaaa actgcctcta cttcgaaacc gggcaaggtt cggcgctgtc tgctggcgct
    50161 aacttcggcg cagactaggt gacgatggaa gcacgtaact atgggctggc gcgtcattac
    50221 gatccgttta tcgtcaacac cgtggtcggc tttattgggc cggagtatct ctacaacgac
    50281 cgccagatta tccgtgctgg cttagaagat cactttatgg gcaagctgag cggcatctct
    50341 atgggctgtg actgctgtta caccaaccac gctgatgctg accagaacct caacgaaaac
    50401 ctgatgatcc tgctcgccac cgcaggctgc aactacatca tggggatgcc gctgggcgat
    50461 gacatcatgc tcaactatca gaccaccgca ttccacgaca ctgccactgt gcgtcagtta
    50521 ctcaacctgc gcccgtcacc ggagtttgaa cgctggctgg aaagcatggg cattatggca
    50581 aacggtcgcc tgaccaaacg ggcgggcgat ccgtcactgt tcttctgatg acgcggagat
    50641 aacacatcat ggatcaaaaa cagattgaag aaattgtacg cagcgtgatg gcgtcaatgg
    50701 gacaagcggc ccccgcgccg tcagaagcaa agtgcgccac cactaactgt gcggcaccgg
    50761 tgacctcgga aagctgcgcg ctggatttag gttctgcgga agcaaaagcg tggattggtg
    50821 ttgaaaaccc gcatcgcgca gacgtattaa cagaactgcg ccgcagcacc gtggcccgcg
    50881 tttgtaccgg tcgtaccggt ccgcgtccac gtacgcaggc gctgctgcgt ttcctggccg
    50941 atcactctcg ttcgaaagat accgtactga aagaagtgcc ggaagagtgg gtgaaagcgc
    51001 agggcttgct ggaagtacgc tccgaaatta gcgacaaaaa tctctacctg actcgcccgg
    51061 atatgggccg ccgcctgtgt gcagaagctg ttgaagcgct gaaagcacag tgtgttgcta
    51121 atccggacgt acaggtcgtt atttctgatg gcctgtcaac tgatgccatc accgtgaact
    51181 acgaagagat cctgccgccg ctgatggcgg gcctgaaaca ggccgggctg aaagtcggca
    51241 cgccattctt tgttcgttat ggtcgcgtga agattgaaga tcagatcggt gagatcctcg
    51301 gcgcgaaagt ggtgatcctg ctggtggggg aacgtccggg gttgggtcag tcagaaagcc
    51361 tctcctgtta cgccgtttac tcgccgcgta tggcgaccac cgttgaggcc gatcgcacct
    51421 gcatctctaa cattcaccag ggcggcacgc cgccggttga agccgcagcc gtcattgtgg
    51481 atttggcgaa acgtatgctg gagcagaaag catccggcat caacatgacc cgataaggag
    51541 gcatcatgcc agctttagat ttgattcgac cgtcggtaac cgctatgcgg gtgattgcct
    51601 ccgttaacgc tgaatttgcg cgtgaactaa aattgccgcc gcatattcgt agcctcgggc
    51661 tgatttctgc tgattccgat gacgtcacct acattgcggc tgacgaagcg accaagcagg
    51721 cgatggtcga agtggtatat ggccgctcgc tgtatgcggg agcagcacac ggaccgtcac
    51781 cgactgccgg tgaagtgctg attatgcttg gtgggccaaa cccggcggaa gtgcgtgctg
    51841 gtctggatgc gatggttgcg catattgaaa atggcgctgc tttccagtgg gctaatgacg
    51901 cagaaaacac cgcattcctg gcacatgtag tttcgcgtac cggttcttat ctttcatcaa
    51961 ccgccgggat cacgcttggc gatccgatgg cgtatctggt ggcaccgccg ctggaagcga
    52021 cctacggcat tgatgcagcg ttgaaatctg ccgacgtaca gctggtgacc tatgtcccgc
    52081 caccgtctga aaccaactac tcggcagcat ttttaaccgg tagccaggcc gcgtgtaaag
    52141 cagcctgtaa cgcctttacc gatgcagtgc tggaaatcgc gcgtaatcca atccagcgtg
    52201 cgtaacggag gttgccgatg atcaatgcac tgggattgct ggaagtggac ggcatggtcg
    52261 ccgcgataga tgcagcggat gccatgctca aagcagctaa cgttcgtctg ctcagtcacg
    52321 aagtgcttga ccctggtcgc ttaacgctgg tggtggaagg cgatctggcg gcgtgtcgtg
    52381 cggcgctgga cgcaggttgt gctgccgcga tgcgtaccgg gcgtgtcatc agccgcaagg
    52441 agatcggtcg gccagacgat gacacccagt ggctggttac tggctttaac cgccagccga
    52501 agcaacccgt aaaggaaccc gacgcgccag ttatcgtcgc ggaatctgct gacgagttgt
    52561 tggcgctgtt aacatcagta cgtcagggaa tgacggcagg agaagtggct gcccactttg
    52621 gctggccgct ggaaaaagcc agaaatgcgc tcgaacagct cttttctgcc gggacgttac
    52681 gtaaacgcag tagtcgttat cgtctcaagc cccattaacc tgtcggaggt gccgggtgtc
    52741 ctacaacacc cggcattaac atcatgaaaa agacccgtac agccaatttg caccatcttt
    52801 atcatgaacc cttacccgaa aacctgaagc tcacgccgaa ggtcgaagtg gataatgttc
    52861 atcaacgaca gacaacggat gtctatgaac atgctttgac gattaccgcc tggcagcaga
    52921 tttacgatca gctgcatccg ggcaagtttc atggtgaatt tacggaaatt ctgctcgatg
    52981 atattcaggt ttttcgtgaa tacaccggtc tggcgctgcg tcagtcgtgc ctggtctggc
    53041 cgaactcgtt ctggtttggc attccggcga cgcgcggtga gcagggattt atcggttcgc
    53101 aatgtctggg aagcgcagaa atagcgacgc gccccggtgg aaccgagttt gaattaagta
    53161 cgccggacga ttatacgatc ctgggcgtag tgctgtctga agatgtcatc acccgtcagg
    53221 ctaacttttt gcacaatccg gatcgggtat tacatatgct gcgtagccag tcggcgctgg
    53281 aagtgaaaga gcagcataaa gccgcgctgt ggggctttgt ccaacaggcg ctggcgacgt
    53341 tttgcgagaa cccggaaaat ctccatcagc ctgcagtgcg aaaagtgctg ggggataatt
    53401 tgctaatggc gatgggggcg atgctggaag acgcgcaacc aatggtgacg gcggaaagca
    53461 tcagtcatca gagttaccgt agattacttt cccgcgcccg tgaatatgtg ctggaaaata
    53521 tgtctgagct ggtgacggtg ctggacttgt gtaatcaact gcatgtcagt cgccgcacgc
    53581 tacaaaacgc gtttcacgct attttaggca ttggcccaaa cgcgtggctg aaacgcattc
    53641 gcctgaacgc cgtacgccgc gaactgataa gcccgtggtc gcaaagcaca acggtaaaag
    53701 acgccgccat gcagtgggga ttctggcatc tggggcaatt tgccacggat taccagcagc
    53761 tgtttgccga gaagccgtcg ttgacgttgc atcagcggat gcgggaatgg gggtgaggga
    53821 gttacaccca atccctgacc ttaataaact cacttaacgc cgcttctggg ctgccatctt
    53881 ttggctgata atcatactcc cagcgtacca gtggcggcat tgacatcagg atcgactcgg
    53941 tgcgcccgcc agtttgcagg ccaaacagag tgccgcgatc ccagaccaga ttgaactcga
    54001 cataacgacc gcgacggtag agctgaaaat tgcgctcgcg ctcgccgtag gccatcgctt
    54061 tacgtcgctc tacaattggt aaataagcgt cggtgtagcc tttgcctacc gcctgcataa
    54121 aggcaaaaca gtggtcgaaa tctggcgtgt tcagatcatc aaagaacagc ccgccaatac
    54181 cgcgctgttc gttgcgatgt ttgaggtaga agtaatcgtc gcaccacttt ttgtaacggg
    54241 gataaacgtc ttcaccaaat ggcaggcaca ggtcacgggc ggtgcggtgc cagtgaatgg
    54301 cgtcttcttc aaaaccatag aaaggggtta aatcgaagcc gccgccaaac caccagacgg
    54361 gctctgcacc cggtttttcg gcaataaaaa aacgcacatt cgcgtggctg gtgggaacat
    54421 acgggttatg cggatgcact accagtgaaa cgcccatcgc ctcgaaactg cgcccggcaa
    54481 gttccgggcg atgagcggtg gcggaagcag gcatcgcctc gccgtggacg tgagaaaagt
    54541 tgacgcctgc ctgttcgaaa acaccaccat tacgcaacac ccgactacgc ccgccgccgc
    54601 cagcttcgcg ctgccaacta tcttcgacaa attctgcgcc atcgacggcg gtcagctgct
    54661 gacaaatcgt atcctgaagg ttgagcagaa actgtttaac ctggtgtgcg tcgggtttca
    54721 taacttatcg ctttttcgaa tgtgctttct ggttgtcgaa ccagtggaaa taactgatca
    54781 cgccttcagc aatcgctgtg gcgattttct gacgaaacgc caccgtgcct aacagccgct
    54841 cttcttccgg gttggtgata aacgaggttt ccaccagcac cgaaggaacc gacggcgatt
    54901 tcaacaccac aaacgcggct tgttcggtgt tgcggctgtg cagtttatgc accggcttaa
    54961 tctttttcag aatatgcgag ccaagcgtca ggctgttttt aatagtatcg gtttgcacca
    55021 gatcaaacag cacttgctgc aataggtgat ccttgtcagt cgccttttta ccggcaactt
    55081 catcggcgcg gttttcgcgt tcagacaggt atttcgccat tgcgctactt gccccacggt
    55141 tagagagggc aaataccgaa gcaccggcag ctttcgggtt ggtaaagcca tcggcatgaa
    55201 ttgacataaa cagatctgcg ccatgtttat gggcgatttc aacgcgatcg taaagtggga
    55261 taaacgtatc gccagaacgc gttaaacgcg catcaatccc atgattacgc aaaatggaac
    55321 ggacgttttt agcaatcgcc agcaccacat gtttttcttt cgaaccgttg cgtccgatcg
    55381 ctccggtatc aattccgccg tggcctggat cgagaacaac gacacgtttg ccgccagatt
    55441 ttttggcttt cggcttgctg tgtccgttgc tggtttttaa aggttcgtct ttggcgatgg
    55501 cttgcgacat tcctgacaac gtcagggcag ccaaaccggc tttcagcacc tggcggcgcg
    55561 aagtgagtgt ttttagtggt ttaaaagtgc tcatacggcc tgagttgtaa taataaagtt
    55621 ccagatgtta tatcgtatcg cgtaatccgt tacgacggtt tgatgttgag aattgtttta
    55681 cttttcattt caatagttga caactcccca ttatgccact ttttcaaccg attttcgtca
    55741 gatattggct aattcgaccg ttcacgccat aatcagcctt ttaacccgca aaaaggtaac
    55801 gaataccatg gagatacgcg tatttcgcca ggaagatttc gaagaggtca tcaccctttg
    55861 ggagcgttgc gacttgctgc gtccgtggaa cgatccggaa atggacatcg agcgtaagat
    55921 gaaccatgac gtcagtttgt ttttggtcgc tgaggtaaac ggtgaagtgg tcggaacggt
    55981 gatgggcggt tatgacgggc atcgcgggtc tgcttattat cttggcgtgc atccagagtt
    56041 tcgtgggcgt gggattgcca atgcgttgct taatcggctg gagaaaaagc tgattgctcg
    56101 tggctgcccg aaaattcaga tcaatgtgcc ggaagataac gacatggtgc tcggaatgta
    56161 tgaacgcctg ggatatgaac acgccgacgt gctgagtctg ggtaagcgtt tgattgaaga
    56221 tgaagagtac tgagttccat cctgtccatt atgatgcgca cggacgcctg cgtttaccct
    56281 tgctcttctg gcttgtgcta ttgcttcagg cgcgaacctg ggtgctgttt gtcattgccg
    56341 gtgcgtcgcg cgagcagggt actgcgctgc taaatctgtt ttatcccgat cacgataatt
    56401 tctggctggg gctgattcca ggcattccgg cggtgctggc gtttttgttg agcggtcggc
    56461 gggctacatt tcctcgcact tggcatgtgc tctattttct gttgctgttg gcgcaggtgg
    56521 ttttactttg ctggcaaccg tggctgtggc tgaacggtga atccgttagc ggtatcggtc
    56581 tggcgctggt agtggcggat attgtggcgt taatctggct gctgaccaat cgacgtttac
    56641 gcgcttgttt taatgaagaa aaagaataac ggcacttttt ggtgaatttg cactccaagc
    56701 aacgttattg aataaccaaa ggaagtgaca tgaaatcgct gcgtttaatg ttatgcgcta
    56761 tgccgttgat gctgaccggc tgttccacga tgtcgtcagt taactggtcg gccgctaacc
    56821 cgtggaactg gtttggatcg tccaccaaag tgagcgagca gggcgtgggt gaattaacgg
    56881 cgtccacacc actgcaagaa caagccattg ctgatgcgct tgatggcgat tatcgcctgc
    56941 gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt ttttgaagtg atgaaaggcg
    57001 acaacgtggc gatggtgatt aacggcgatc aaggcacgat cagtcgcatt gacgtgctgg
    57061 atagcgatat tcctgctgac accggtgtta aaatcggtac accgtttagc gacctttaca
    57121 gcaaagcatt tggcaattgc caaaaagctg atggtgatga taatcgtgcc gtcgaatgta
    57181 aagccgaagg cagtcaacat attagctacc agttcagcgg ggaatggcgt ggtcctgaag
    57241 ggttaatgcc ttcggacgat accctgaaaa actggaaagt cagtaaaatt atctggcggc
    57301 gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca cgtaaaatag cgctcaacaa
    57361 tgccacggat tgcgtggcat tcttattttc aggaggaaca atgtctcagg ttcagagtgg
    57421 cattttgcca gaacattgcc gcgcggcgat ttggatcgaa gccaacgtga aaggggaagt
    57481 tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa ctggcaactt ttgaagcgaa
    57541 attcccggac gcgcatcttg gtgcggtggt tgcctttggt aacaacacct ggcgcgctct
    57601 gagcggcggc gttggggcag aagagctgaa agattttccg ggctacggta aaggccttgc
    57661 gccgaccacc cagttcgatg tgttgatcca cattctttct ctgcgtcacg acgtaaactt
    57721 ctctgtcgcc caggcggcga tggaagcctt tggtgactgc attgaagtga aagaagagat
    57781 ccacggcttc cgttgggtgg aagagcgtga cctgagcggc tttgttgacg gtacagaaaa
    57841 cccggcgggt gaagagacgc gtcgcgaagt ggcggttatc aaagacggcg tggatgccgg
    57901 cggcagctat gtgtttgtgc agcgttggga gcacaacctg aagcagctca accggatgag
    57961 cgttcacgat caggagatga tgatcgggcg caccaaagag gccaacgaag agattgacgg
    58021 cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat ctgaaagaag atggcaaagg
    58081 gctgaagatt gttcgccaga gcctgccgta cggcaccgcc agtggcactc acggtctgta
    58141 cttctgcgcc tactgcgcgc gtctgcataa cattgagcag caactgctga gcatgtttgg
    58201 cgataccgat ggtaagcgtg atgcgatgtt gcgtttcacc aaaccggtaa ccggcggcta
    58261 ttacttcgcg ccgtcgctgg acaagttaat ggcgttgtaa gcgtttctga ttcgatgatt
    58321 aaaggccagc cagtaacaaa actgactggc ctttttatta cttttgcttc aacgccgcat
    58381 acacctgatc aactacccaa ccataagttg caatcggtaa ctgaccgctt tcgaacatat
    58441 tgggattttt ttgtggatcg gcaactggcg aatggggctt gttgcttaac atcacaatcg
    58501 ccatatgatt gaccggatcg ataacggtta ctgtcccggt ccagccagtg tgaccgtagg
    58561 tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt ggcattacca ttcacgcgcc
    58621 agccgaggcc aaaagtggca tcttccttag agctggtggt gaacatcttc accgtttccg
    58681 cactgaacag ctgcacatcg ccatagcccc cgccgttcag catcgtttgc attaacaccg
    58741 caatatcgcc ggtattggaa aacaaacctg cgtgcccgga aacgccgccc atcgaataaa
    58801 aggctttttc atcgtgcacc tgaccccaga gagtggaggt gcggatattc gggaaatgga
    58861 tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc aatttgttgc ggtttaaagc
    58921 ctttcagcag cgggttaaat accgtatgtg tcaggccgag cgggcgataa atcgactctt
    58981 caacatagcg gtcaagcggc tgaccggtaa ccgactcgac gataaatcca agcagcatat
    59041 aatcgacatc gctgtagata tgtttgctgc cgggctgata ttccagcggc gtgcgcttga
    59101 tcatctccag cgtctggcct ttatcctggg aatataacgc gcccgcgacc gctttattcg
    59161 ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag caggtcagaa atccgcaggg
    59221 tgtttttgcc tttaatggtg tcatttggac tatcggcaaa ccccggaata tatttcgcaa
    59281 tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt ttgcagggcg aagttcgtgg
    59341 cgtacatttt ggtgtttgag gccaggtcat acagcgtccc ggtggtggct ttgacaggct
    59401 gttccatcaa cacgctgcca tcgtactttt tcgccgctcc ccaggctttg cgatacacaa
    59461 tctgattatc tttaatgatc agcaggttta cgccgggata accgacatca atttgctggc
    59521 taatccagcg atccatctgg ttaagccgtt cgacgttaaa ccctgctttc tctggcgagc
    59581 tttctgtcag aacagggtat tttgcggcgc tgacgctaca actaacagcc agcagagaaa
    59641 gatagagcat tgtccgtttc atgatatcag tccagattga cgttacggca gccaaagagc
    59701 gtggtgaaaa taaacccgca aacccatgcc accagaatcc cgccagcata aaccgccatg
    59761 gccggaagga tgccttgtgc ggaagtcatc agcggcagcg ctaccagacc agacggccca
    59821 aaggcgctgt ttaagcccat cggcagaccc catcaggcta tcaggccgat aaacaaaccg
    59881 cccgccgcgc cgcctaaaca ggcggtaaca aacggcttca tgcggggcag ggtgacaccg
    59941 taaatcagcg gttcaccaac gcccagcagg ccgggaataa tcgccccgcg tacctgactg
    60001 cgtagcgcac tgtgcggttg cgcccgccag tagagtgcca gcgccgcgcc cacctggccc
    60061 gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc cctagctgtc cattaacgcg
    60121 aggtaaacag gaataaagcc ctgatgcacg ccaaatacca cggcgatcag gaacagcccg
    60181 gctaaaaccg cacaaccgaa cggattactg ttcaggtgca taaacagcca tgacatacct
    60241 tcgaataacc agccacccag cggcataatg atcaggtacg caagcgtagc ggtgatcagc
    60301 agggtgatta acgaggtcag cagcatgtcg agatcgtccg acataaagcg gcgcaccatg
    60361 ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc cgataatatt gccgcgcgga
    60421 tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt caccggtggt tgcagcgggg
    60481 ttataaccga gcaaaaagag tgcggcaata attgcgccat tgacgcccgt gccgccaaat
    60541 gcctgggcgg cgttatagcc caccagaatc accaggaaag tgaacaaacc tttgctgaac
    60601 accttcataa aattcagcgc gtcgggtagc gtgccctgag catctgccgg aacgtgcatc
    60661 accgtggcaa ttaacgtcgc tatccccagc agcagaccgg cggcaataaa accggggatc
    60721 agcggcgtga agatggtggc gaatttggcg agaaattgtt gcacgccgga ggtttgtttg
    60781 gcttttaact gacgtttgtt ctgggcggcg atttctgcgg catcctgtac cggtgcctcg
    60841 cccagcagct cgctcatcgc ttttgcggca cgatgcgctt taccaggccc aaaaacgacc
    60901 tgcacctggt cgctggtcaa aatgacgccc ttcacgcctt ccagcgtttt gatattggga
    60961 tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg tcatacagtt accacaactg
    61021 gcgatatttc ccagtccgcc gacacgggta agaatggtgt tcagaagttc actgctgatc
    61081 tctttggcca tgggttattc cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc
    61141 aggcgttttt tagcttctgc ggcatcgaga tttttcagca ccatcacaat ggccgtttta
    61201 cagttgcgct cgcaggcaat taacgccgct tccgcttgtt cggcattaca tccggtggcg
    61261 tttttaacga tattgacctg gcgcacatgc agtttttcgt tggtggcgac cacatcgacc
    61321 atcaggttgc cgaacacttt gccggattta atcatcagcc cggtggaaag catattgagc
    61381 accagtttct gcgctgtgcc tgctttcatt cgcgaagaac cagtgaccac ttcggcacct
    61441 acaatcggtg taatggcaaa ctcggcggtg gtggaaacgg cactccccgg attacaggaa
    61501 attcccactg tgcggcaacc gagctggcgg gcgtattcca gtccggcaat cacatacggc
    61561 gtacgaccgc tggcggcaat accgaccacc acatcctgtg ccgttaaatt tatatttttc
    61621 agatcgttaa tgccgccttc tcggctatct tccgtgcctt ccaccgcgtg ctgaatggca
    61681 tattcgccgc cagcaatcaa accaaccacc agacccggtt tcacgccgta ggtgggcgga
    61741 cattcgctgg catccagaat ccccagacga ccggatgttc ccgcaccgag ataaatcaga
    61801 cgaccgccgc cgcttacctg ggcgtggata acatcgatcg ccgcggcgat atccggcagg
    61861 acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt tgataatccg gcacatttcc
    61921 agcgtcgata cgcggtcaat ttcagccgag gcggtgttcg agccttcagt aatcatcttt
    61981 tcaagttgca tcggtggtga ccttactatt tacgatcaaa taattgcaga tgtatggaat
    62041 catatatccc gaattgacag acttaaagga atcattgagt ctacacttgc agagtgtgaa
    62101 gagcgtcata agaacttaag cggaattttt gctgccagcc cgggagagtg gaagatgttg
    62161 tacctgacga aaatacgcaa tgcggaaagt gaatttaccg agaacgagca aaaaattgct
    62221 gactttctgc gggccaacgt gagtgagctg aaatccgtct cttcgcgcaa gatggcgaaa
    62281 cagctcggca tcagtcagtc gagcattgtg aaatttgccc agaagctcgg cgcgcaagga
    62341 tttactgaat tgcgtatggc gttaattggc gaatacagtg ccagccggga gaaaacgaat
    62401 gccacggcgc tgcacctgca cagttcgatt accagtgatg attcgctgga agtgattgcc
    62461 cgcaaactga atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt gttcgactac
    62521 gctcggctgc aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca gataacgggc
    62581 ctgggcgggt cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa aattggctat
    62641 cgcgtggcct atgaagccga cacacacgta caagccaccg tttcccaggc gttgaaaaaa
    62701 ggcgatgtac aaatcgctat ctcttacagc ggcagtaaaa aagagattgt cttgtgcgca
    62761 gaagcggcac gaaagcaggg ggcaacggtg attgccatta cttctctggc tgactctccg
    62821 ctgcggcggc tggcgcattt cacgctcgat accgtttccg gtgaaaccga atggcgtagc
    62881 tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg atttactgtt tgttggtctt
    62941 gtgcaactca atgatgtgga gtcattgaaa atgattcagc gcagcagtga gctaacacaa
    63001 cgcctgaaat gatgcataaa gcagcgactg gattgagatt ttcctgaatt agtgagctga
    63061 tccgcagcaa tattttgttt atcctgtatt ttcagaggga atggagtgta acgctctgta
    63121 ttaacaagga gagcattaaa atgggtaaac tcacgggcaa gacagcactg attacgggcg
    63181 cattgcaggg aattggcgaa ggaattgcca gaacttttgc gcgtcatggc gcaaacctaa
    63241 tcttgctgga tatctcccct gagatcgaaa agctggcgga cgaactgtgt ggtcgtggtc
    63301 atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc gtcggtagcc gcagctatca
    63361 aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt gaataacgca ggcgtttgtc
    63421 gtctgggcag tttcctcgat atgagcgatg acgatcgcga tttccatatt gacatcaata
    63481 ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga gatgattgcc cgcaaagatg
    63541 gtcgcattgt gatgatgtct tcagtcactg gtgatatggt ggccgatcct ggcgaaacgg
    63601 cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa atcgctggcg gtggagtacg
    63661 cgcagtctgg tattcgcgtt aacgccattt gcccgggata cgtgcgcaca ccaatggcgg
    63721 aaagcattgc ccgccagtcg aacccggaag atccagagtc ggtgctgact gaaatggcga
    63781 aagcaatccc gctgcgtcgc ctcgccgatc cgctggaagt cggcgaactg gcggccttcc
    63841 tcgcatcgga tgaatccagc tatttaaccg gtacacagaa tgtgattgat ggcggcagca
    63901 cactgccgga gacggttagc gtcggtatct gattcacctc tgtttcctcc ctgcatttgt
    63961 ggggaggatt tcgttttaaa cccggttctt attccctttt caacttccaa atcaccaaac
    64021 ggtatataaa accgttactc ctttcacgtc cgttataaat atgatggcta ttagaaagtc
    64081 attaaattta taagggtgcg caatggccgt taacttactg aaaaagaact cactcgcgct
    64141 ggtcgcttct ctgctgctgg cgggccatgt acaggcaacg gaactgctga acagttctta
    64201 tgacgtctcc cgcgagctgt ttgccgccct gaacccgccg tttgagcaac aatggaccaa
    64261 agaaaacggc ggcgacaaac tgacaataaa acaatctcat gccgggtcat caaaacaggc
    64321 gctggcaatt ttgcagggct taaaagcaga cgttgtcact tataaccagg tgactgacgt
    64381 acaaatcctg catgacaaag gcaagctgat cccggcggac tggcaaacgc gcctgccaaa
    64441 taatagctcg ccattctact ccaccatggg cttcctggtg cgtaagggca acccgaagaa
    64501 tatccacgac tggaacgacc tggtgcgctc cgatgtgaag ctgattttcc cgaacccgaa
    64561 aacgtcgggt aacgcgcgtt acacctatct ggcggcatgg ggcgcagcgg ataaagctga
    64621 cggtggcgat aaagccaaaa ccgaacagtt tatgacccag ttcctgaaaa acgttgaagt
    64681 gttcgatact ggcggtcgtg gcgcgaccac cacttttgcc gagcgcggcc tgggcgatgt
    64741 gctgatcagc ttcgagtcgg aagtgaacaa catccgtaaa cagtatgaag cgcagggttt
    64801 tgaagtggtg attccgaaaa ccaacattct ggcggaattc ccggtggcgt gggttgataa
    64861 aaacgtgcag gccaacggta cggaaaaagc cgccaaagcc taccttaact ggctctacag
    64921 cccgcaggcg caaaccatca tcaccgacta ttactaccgc gtgaataacc cggaagtcat
    64981 ggataaactg aaagacaaat tcccgcagac cgagctgttc cgcgtggaag acaaatttgg
    65041 ctcctggccg gaagtgatga aaacccactt caccagcggc ggcgagttag acaagctgtt
    65101 agcggcgggg cgtaattaat gtttgcagtt tcatcaagac gcgtgctgcc gggctttacc
    65161 ttaagcctcg gcaccagtct gctgtttgtg tgcctgattt tgctgctgcc gctctccgcg
    65221 ctggtgatgc agctggctca gatgagctgg gcgcagtact gggaggtgat caccaacccg
    65281 caggtagtag cggcctacaa agtaacgctg ctgtcagcgt ttgtggcatc gatttttaac
    65341 ggcgtttttg gcctgctgat ggcgtggatc ttaacccgct atcgctttcc gggccgcacg
    65401 ctgcttgatg cgctgatgga tttacccttt gcgctgccaa cggcagtcgc cggtttaacg
    65461 ctagcctcgc tcttttctgt aaacggtttt tacggtgaat ggctggcgaa gtttgatatc
    65521 aaagtcacct atacctggct ggggattgcg gtggcgatgg cctttaccag cattccgttt
    65581 gtggtacgta ccgtgcagcc ggtgctggaa gagttaggcc cggaatatga agaagcggca
    65641 gaaacgcttg gcgcaacgcg ctggcagagt ttctgcaaag tggtgctgcc ggaactttct
    65701 ccggcgctgg tggcgggcgt ggcgctgtcg tttacccgta gtcttggtga atttggcgcg
    65761 gtgattttta tcgccggaaa tatcgcgtgg aagacggaag tgacgtcgct gatgattttt
    65821 gtgcgcttac aggagtttga ttacccggca gcgagcgcga ttgcttcggt gatcctcgcg
    65881 gcatctctgc tgctgctgtt ctcaattaac actctgcaaa gtcgctttgg tcggcgtgtg
    65941 gtaggtcatt aatggcggaa gttacccaat tgaagcgtta tgacgcgcgc ccgattaact
    66001 ggggcaaatg gtttctgatt ggcatcggga tgctggtatc ggcgttcatc ctgctggtgc
    66061 cgatgattta tatcttcgta caggcgttca gcaaagggct gatgccggtt ttacagaatc
    66121 tggccgatcc ggacatgctg cacgccatct ggctgacggt gatgatcgcg ctgattgccg
    66181 taccggtaaa cctggtgttc ggcattctgc tggcctggct ggtgacgcgc tttaacttcc
    66241 ctggacgcca gttactgctg acgctactgg acattccgtt tgccgtatcg ccggtggttg
    66301 ccggtctggt gtatctgctg ttctacggct ctaacggccc gctgggcggc tggctggatg
    66361 aacataatct gcaaattatg ttctcctggc ccggaatggt gctggtcacc atctttgtga
    66421 cgtgtccgtt tgtggtgcgc gagctggtgc cggtgatgtt aagccagggc agccaggaag
    66481 acgaagcggc gattttgctt ggcgcgtctg gctggcagat gtttcgccgc gtcacattgc
    66541 cgaacattcg ctgggcgctg ctttatggcg tggtgttgac caacgcccgc gcaattggcg
    66601 agtttggcgc ggtgtcggtg gtttccggct cgattcgcgg cgaaaccctg tcgctgccgt
    66661 tacagattga attgctggag caggactaca acaccgtcgg ctcctttacc gctgcggcac
    66721 tgttaacgct gatggcgatt atcaccctgt ttttaaaaag tatgttgcag tggcgcctgg
    66781 agaatcagga aaaacgcgca cagcaggagg aacatcatga gcattgagat tgccaatatt
    66841 aagaagtcgt ttggtcgcac ccaggtgctg aacgatatct cactggatat tccttcaggt
    66901 cagatggtcg cgttgctggg gccgtccggt tccgggaaaa ccacgctgct gcgcattatc
    66961 gccgggctgg agcatcaaac cagcgggcat attcgcttcc acggcaccga cgtcagccgc
    67021 ctgcacgcgc gcgatcgcaa ggtcggtttc gtgttccagc attacgcgct gttccgccat
    67081 atgacggtat tcgacaatat cgcttttggc ctgacggtgc tgccgcgtcg cgatcgcccg
    67141 aatgccgcag ccatcaaagc gaaagtgaca aaattgctgg agatggtgca gcttgctcat
    67201 ctggcggatc gttatccggc acagctttcc ggcggccaga aacagcgcgt ggcgctggcg
    67261 cgcgcgctgg ctgtagaacc gcaaattctg ctgcttgatg aaccgtttgg cgcgctggat
    67321 gcgcaggtgc gtaaagagct gcgtcgctgg ctgcgtcaac tgcatgaaga gctgaaattc
    67381 accagcgtgt tcgtgaccca cgaccaggaa gaagcgacgg aagtagctga tcgtgtagtg
    67441 gtgatgagcc agggcaatat tgaacaggct gatgcgccgg agcaggtatg gcgcgaacca
    67501 gcgacccgtt ttgtgctcga atttatgggc gaagtgaacc gcctgcaggg aaccattcgc
    67561 ggcgggcagt tccatgttgg cgcacatcgc tggccgctgg gctacacacc tgcttatcag
    67621 gggccggtgg atctcttcct gcgcccgtgg gaagtggata tcagccgccg taccagtctc
    67681 gattcgccgc tgccggtaca ggtactggaa gccagcccga aaggtcacta cacccaatta
    67741 gtggtgcagc cgctggggtg gtacaacgaa ccgctgacgg tcgtgatgca tggcgacgat
    67801 gccccgcagc gtggcgagcg tttattcgtt ggtctgcaac atgcgcggct gtataacggc
    67861 gacgagcgta tcgaaacccg cgatgaggaa cttgctctcg cacaaagcgc ctgataggtt
    67921 gagtgaatgt taaacgcccg gaggcgcttc ccgcgatccg ggctttttaa tggcaaggtt
    67981 tgtaacctgt agacctgata agacgcgcaa gcgtcgcatc aggcaacacc acgtatggat
    68041 agagatcgtg agtacattag aacaaacaat aggcaatacg cctctggtga agttgcagcg
    68101 aatggggccg gataacggca gtgaagtgtg gttaaaactg gaaggcaata acccggcagg
    68161 ttcggtgaaa gatcgtgcgg cactttcgat gatcgtcgag gcggaaaagc gcggggaaat
    68221 taaaccgggt gatgtcttaa tcgaagccac cagtggtaac accggcattg cgctggcaat
    68281 gattgccgcg ctgaaaggct atcgcatgaa attgctgatg cccgacaaca tgagccagga
    68341 acgccgtgcg gcgatgcgtg cttatggtgc ggaactgatt cttgtcacca aagagcaggg
    68401 c
//
LOCUS       DAJVXP010000006        64816 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVXP010000006 DAJVXP010000000
VERSION     DAJVXP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.006"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="OQS10_000402"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="OQS10_000402"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS2291533.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="OQS10_000403"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="OQS10_000403"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS2291534.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="OQS10_000404"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="OQS10_000404"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS2291535.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="OQS10_000405"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="OQS10_000405"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS2291536.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="OQS10_000406"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="OQS10_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS2291537.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="OQS10_000407"
     CDS             complement(4809..7043)
                     /locus_tag="OQS10_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS2291538.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="OQS10_000408"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="OQS10_000408"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS2291539.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="OQS10_000409"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="OQS10_000409"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS2291540.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="OQS10_000410"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="OQS10_000410"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS2291541.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKTGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="OQS10_000411"
     CDS             complement(12798..14138)
                     /locus_tag="OQS10_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS2291542.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="OQS10_000412"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="OQS10_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS2291543.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="OQS10_000413"
     CDS             complement(15927..16376)
                     /locus_tag="OQS10_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS2291544.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="OQS10_000414"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="OQS10_000414"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS2291545.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="OQS10_000415"
     CDS             complement(17176..17505)
                     /locus_tag="OQS10_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS2291546.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="OQS10_000416"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="OQS10_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS2291547.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="OQS10_000417"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="OQS10_000417"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS2291548.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="OQS10_000418"
                     /gene_synonym="ygdH"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="OQS10_000418"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS2291549.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="OQS10_000419"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="OQS10_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS2291550.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="OQS10_000420"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="OQS10_000420"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS2291551.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="OQS10_000421"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="OQS10_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS2291552.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="OQS10_000422"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="OQS10_000422"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS2291553.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="OQS10_000423"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="OQS10_000423"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS2291554.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="OQS10_000424"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="OQS10_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS2291555.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="OQS10_000425"
                     /pseudo
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="OQS10_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="OQS10_000426"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="OQS10_000426"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS2291556.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="OQS10_000427"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="OQS10_000427"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS2291557.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="OQS10_000428"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="OQS10_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS2291558.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="OQS10_000429"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="OQS10_000429"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS2291559.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="OQS10_000430"
     CDS             complement(34677..35072)
                     /locus_tag="OQS10_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS2291560.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="OQS10_000431"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="OQS10_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS2291561.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="OQS10_000432"
     CDS             complement(36359..36586)
                     /locus_tag="OQS10_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS2291562.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="OQS10_000433"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="OQS10_000433"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS2291563.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="OQS10_000434"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="OQS10_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS2291564.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="OQS10_000435"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="OQS10_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS2291565.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="OQS10_000436"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="OQS10_000436"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS2291566.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="OQS10_000437"
     tRNA            40666..40742
                     /locus_tag="OQS10_000437"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            40776..40852
                     /locus_tag="OQS10_000438"
     tRNA            40776..40852
                     /locus_tag="OQS10_000438"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40810..40812,aa:Met,seq:cat)
     gene            40886..40962
                     /locus_tag="OQS10_000439"
     tRNA            40886..40962
                     /locus_tag="OQS10_000439"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40920..40922,aa:Met,seq:cat)
     gene            complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="OQS10_000440"
     CDS             complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="OQS10_000440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS2291567.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42522..43853
                     /gene="argA"
                     /locus_tag="OQS10_000441"
     CDS             42522..43853
                     /gene="argA"
                     /locus_tag="OQS10_000441"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS2291568.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43915..45741)
                     /gene="recD"
                     /locus_tag="OQS10_000442"
     CDS             complement(43915..45741)
                     /gene="recD"
                     /locus_tag="OQS10_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS2291569.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45741..49283)
                     /gene="recB"
                     /locus_tag="OQS10_000443"
     CDS             complement(45741..49283)
                     /gene="recB"
                     /locus_tag="OQS10_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS2291570.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="OQS10_000444"
     CDS             complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="OQS10_000444"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS2291571.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52340..55708)
                     /gene="recC"
                     /locus_tag="OQS10_000445"
     CDS             complement(52340..55708)
                     /gene="recC"
                     /locus_tag="OQS10_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS2291572.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55721..56044)
                     /locus_tag="OQS10_000446"
     CDS             complement(55721..56044)
                     /locus_tag="OQS10_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS2291573.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56029..56436)
                     /locus_tag="OQS10_000447"
     CDS             complement(56029..56436)
                     /locus_tag="OQS10_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS2291574.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56433..56996)
                     /locus_tag="OQS10_000448"
     CDS             complement(56433..56996)
                     /locus_tag="OQS10_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS2291575.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56987..57457)
                     /locus_tag="OQS10_000449"
     CDS             complement(56987..57457)
                     /locus_tag="OQS10_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS2291576.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="OQS10_000450"
     CDS             complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="OQS10_000450"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS2291577.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="OQS10_000451"
     CDS             complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="OQS10_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS2291578.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="OQS10_000452"
     CDS             complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="OQS10_000452"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS2291579.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="OQS10_000453"
     CDS             complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="OQS10_000453"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS2291580.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62943..63632
                     /gene="mutH"
                     /locus_tag="OQS10_000454"
     CDS             62943..63632
                     /gene="mutH"
                     /locus_tag="OQS10_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS2291581.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63701..64414
                     /locus_tag="OQS10_000455"
     CDS             63701..64414
                     /locus_tag="OQS10_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS2291582.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64552..64770
                     /gene="ygdR"
                     /locus_tag="OQS10_000456"
     CDS             64552..64770
                     /gene="ygdR"
                     /locus_tag="OQS10_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS2291583.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15709 a        17427 c        15891 g        15789 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ttggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaagact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttat
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagca caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaagcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc gttttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag cgaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgtgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acacgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagtg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccggat tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgccacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaaga
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttccaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagctagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgcg ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattaaaat ttgatgaagt aaagcagtac ggtgacgcgg ggtggagcag cctggtagct
    40801 cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caatcaaatt
    40861 tgatgaagta aaagcaatac ggtgacgcgg ggtggagcag cctggtagct cgtcgggctc
    40921 ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac
    40981 gggtgttttt ttgtttctac tctcccataa aaaaagcgcc attcagcgcc tttttatcat
    41041 ccccttctcg ccagcgttgc cccatcggca aaatacgctt taatccccgc aagaatagac
    41101 tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc aacgttactg
    41161 ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaacccg
    41221 gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt cagcactgct
    41281 ttaccaaact taaggctgtc agcaatggtc agcgactgca ccatatcgaa catggtgtgg
    41341 tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc gttctgggtt
    41401 tgtgccagat attttgccgc agtactggtt gcacctttgg ttgagagcgc aaacaccgag
    41461 gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga tagagacaaa caggtcagca
    41521 cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc
    41581 gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcaaacg gcgagctatt
    41641 tgcaatacca catctttttc gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca
    41701 ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc gccctgcttt acctggttgt
    41761 ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc
    41821 agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga
    41881 cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt
    41941 aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg
    42001 aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga gtaccgagtt caggtttaca
    42061 tcttcgatat ccaccaccac gcgttcaggg ttactcaacg cgaactgctt atatttcagc
    42121 tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc
    42181 acgacctggc taaccgcagc caggctgacc tgacttacgc tcaatagcca catagcgccc
    42241 gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg
    42301 agcaaaagaa atctaagctg tgtaacaagt aaacgactaa tttgaccgga aactttagcg
    42361 aaagacgcat aatctgtcat ctaataaacg gtaaacattc tttttatatt cacggcatta
    42421 ctgataaaaa agtcgctctc gcataaaatt tacacttgca ccctgcgaaa aaacagaata
    42481 aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa
    42541 ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac cggggaaaaa
    42601 cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc agtatcgtta
    42661 atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat ggcgcacgtc
    42721 cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac aagaatatac
    42781 gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg
    42841 atattactgc tcgcctgtcg atgagtctca ataacacgcc gctgcagggc gcgcatatca
    42901 acgtcgtcag tggcaatttt attattgccc agccgctggg cgtcgatgac ggcgtggatt
    42961 actgccatag cgggcgtatc cggcggattg atgaagacgc gatccgtcgt caactggaca
    43021 gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag agctttaatc
    43081 tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgag aagatgattg
    43141 gtttttgctc ttcacagggc gtcactaatg acgacggtga tattgtctca gaacttttcc
    43201 ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa aggcgattac aactccggta
    43261 cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt cgctgtcatt
    43321 taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg
    43381 gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc aacgatattg
    43441 gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggtg cgccgttctc
    43501 gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagctcgat aacacgacta
    43561 ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg
    43621 cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa cgcattgccg
    43681 ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc agtattcact
    43741 ggttccagga acgtggattt accccggtgg atattgattt actgcccgag agcaaaaagc
    43801 agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttaggg taagcgatga
    43861 aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca tccggcaatt acgtttatcc
    43921 ccgcgaacta aacaacgccg ccagaccact gcgccgctca gtacgagtgg cgattgccgc
    43981 acttaatata cgctcatcgg catacagcga cagacggcga cgcgcgcggg tcaccgcggt
    44041 gtataccagc tctcgcgtta ctatcggcgt gcgttggctc ggtaaaatca acgccgcatg
    44101 gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg
    44161 caagcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc agacgcgcgt
    44221 cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa gcgcgctgtc
    44281 attacgggca atcatcaccg gtcgaccttc gtaccaacga gagtgcgaat gacgatgaat
    44341 tttgcgcttc tgttgcataa actgctcaat tcgctcattc agtccagcca cgccaaatgg
    44401 cccttcccgc agggcgcaca aaagttggta ctcattgaac gcctgaatga ttaaatccgg
    44461 ctcggcacgc gcttgcagca gatccagata acgtccataa cccgcaagag cttcctcaag
    44521 catcgcaata taatcttcgc cgctctgtaa aagccgtttt tcaatatcag taaaatcctg
    44581 ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag ctaactgacc
    44641 aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac tgtcgcgcaa
    44701 agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt cccgtcaggc ggcttagctg
    44761 cccggcacgc tcggcggtaa agccggcatt ggcataagcg cagatatcgc ccagcacagc
    44821 cccagcctca accgaggcca gttgatcacg atcgccgaga aagatcactc gcgcatgatc
    44881 gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg atcattgacg cttcatctac
    44941 caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct ggctacccgg
    45001 ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtc tcttttgttc
    45061 atcggtcagc ggtaactgtc gcaaagcctt gccgagagat tcggttaagc gcgcggcagc
    45121 tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtcggcca tttgaattaa
    45181 cgctgccagc aacttcgcca cggtggtcgt tttaccggtg cctgggccgc cggaaatcac
    45241 cgagatccgc cgcgtcagcg ccactgctgc cgccactttt tgccagttaa tttcatcgct
    45301 tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct caatggcatg
    45361 attcacctca ttgaagaagc gcgctaccgt ccgctcgtta caccacatgc gattcaaata
    45421 aagacgatcg ccacagagga tcatcggcgt gggttcatct cccctgctga ccgcttgaga
    45481 agccagcaag gattcttccc aattttgtag ctcaccgatt tcactgacac aggtcgccaa
    45541 cagcggatgc gacgcctcgt tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc
    45601 tccggcatca tgacttaaca gtgccgccgc gagggtgacg gcaggatgtt catctcccgc
    45661 cacggtcagg gcaaattgca catccagcgg gcgtagctgt ttgtgctcca cagcttccag
    45721 taattgcttt tgcaatttca ttacgcctcc tccagggtca taccggcaaa catctcatcc
    45781 atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct
    45841 ttatcaacgc cacgcaggaa cagatagata acgccgccaa agtgacgatc atagtcgtaa
    45901 tcagcaatgc gatggcgcag atagcgatgc agcgccaggg tataaagctg atattgcaga
    45961 tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct
    46021 tcacccagcc agttggattt atagtcgagc aggtaataac gcccttcgtg gcggaatacc
    46081 aggtcgataa agccttttaa catgccgcgt acctgcatga actccagcgg cgggcagcca
    46141 gcggatagcg ggtcaaactg gcgaattagt gcatcaagct ggctggcgat gagcggttcg
    46201 ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag ctgactcagg
    46261 ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc ggtcaacacc
    46321 ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca gttcgggtca
    46381 accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc cggtgacgca
    46441 ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg
    46501 gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg ctgctgcaag
    46561 ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc
    46621 agttctgcag ttaaagcatc attaacctgc cagggctggt tatcaccagt ttgtgccgtt
    46681 tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc tgccgcatct
    46741 tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct
    46801 tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg ccaaaccgaa
    46861 cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcgac ctccgcgagg
    46921 tcgacgcttt ctggcgcagc attaagatcc aaaactgcct caaacgagtg gcgatcgtga
    46981 taaaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac cagcggatat
    47041 tccagccctt tcgatttatg gatcgtgaca atctgcacca gatgtttatc actttcgagg
    47101 cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga taaccagcgt
    47161 accagcgcat gttcactttc cagctgcgtt ccggcttctt gaagcagttc gctgatatgc
    47221 aagatatcgg taagacgccg ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta
    47281 cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca gatttgccga
    47341 taaccatcga actcttcgac taccgcatcc cacgcatgtt cgtcattatt cagcgtttca
    47401 atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag ggtgttctca
    47461 cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt cctgcgcttc cagagtttca
    47521 aaaacactat cgcggttcga aaggtaaacg gaagggattt ccagcaacgt taaggcatcg
    47581 cgcacctggg cggcctcctg gcggctgcgc accagcacgc tgatgtccga agcacgcacc
    47641 ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc ttgtagccag
    47701 tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc aacgccgcag
    47761 ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt aaagacaaaa
    47821 cgtaacgcct gatttttccc ggctgatttc actggaataa acggtatttc gcgaaacatg
    47881 aacgcgtcat cagtctggct gaaaagcttg ttcacactgt tcaccattcc aggtgcggag
    47941 cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac tacgcgcctt catataagtg
    48001 aagatatccg caccccggaa tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat
    48061 gcggtttctg gctgatggtg ccagatacgg cgaaaaattc ggtactgctg ggggtcggta
    48121 tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc
    48181 tcgccgcttt cgctacgcag cgcggaatcg agccgactta acatgtcatc aaaacccaat
    48241 tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg
    48301 atcaccagat cacggatcga caatggttct gcaagcagtt gatcgatcgc ctcaaacagt
    48361 ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt ctaagaaacg ctgggagaat
    48421 ttttccagcg actccggcaa ctgataactg tttgtctctt cttctgccca ggcgctgatc
    48481 ttgtcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat accagaagat
    48541 tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac cgtatcaata
    48601 cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat catcgggcgg cggtgctttg
    48661 ataaccggcg cttcgccttg cagataacga ttaatatcgc gcagcaacgc ctgcggccct
    48721 ttccaggttt caaagacgac ctgggctatt tcacgcggca gcgggtagca gtggcgacgc
    48781 cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag ctgctgctca
    48841 aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata
    48901 gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg cgcggcttgc
    48961 gctttatcgt cgacctcttc cagcaggtgt tcgtacaatg gattgtcggt ggtttcacgc
    49021 agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa ttctgccgtg
    49081 gcagcctcgg taaaggtgac caccagcagt tcttcaacgg tcagcgggcg gggaaaggcg
    49141 gcggaaccgc ctagtccaag taacaggcgc aaataaagcg ccgcaatcgt aaaggttttg
    49201 cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg caaaggatct
    49261 agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt gctgcaacgc
    49321 gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt tcccgttttg
    49381 gctgccggaa atctgcgaca ggatagccat gccttgcggc tcgactaccg cctgatggaa
    49441 gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg gccacgattt tatcacgcga
    49501 atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt cttcgccgag
    49561 cgtttgcggt gcctgcagca tctgggtaat taccgcctgc tggatttgcg caaactcatc
    49621 tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa aacgccttgt aacgctccca
    49681 caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc actgacgccc
    49741 cacgctcatt ggaaacgcaa acacggcata gcccaattgt tcttcggtac gcaactgatt
    49801 gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc tggtgtattc
    49861 atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt taccggcttt
    49921 ttcaaagatg acggattgtt ttttatcgac cactacatct ttgtttcgac accactctga
    49981 accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct gggcctcggt
    50041 catgttgccg ataaccataa actctggtcg agcccctgat tttaaggcgt cgcgataagc
    50101 cagcacctct ttcaacgtaa tggagggcaa aattttacgc cgttcatctc gcgagaagta
    50161 cggcacttgc gagagcatct gcgcgggcat aatcgcctgc tcaaacgctt tacccttttc
    50221 tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgat cttccgtagc
    50281 ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac gctgggtgta
    50341 accattagca ttaaccataa ggccgttgtt agcgttggtg gaaaaactta tgccaccaac
    50401 cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg agataatcat tgagcgcaaa
    50461 catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca ggctgacatc
    50521 agctttgggt tcgctggaaa aataacggct tggcgcatac accacgcgca gattcgactc
    50581 atcaacaatc agctctggat ggtcatattt cttctctgac ttaatcagcg agaaatcgtc
    50641 aggaatatag gggttaagct ccggtaagga gagcgcaata ttggcagctt tttgctgcca
    50701 gtcagcgaaa gtttgttcgc tgattttatc gacctgatac ggcgcatcga caaaataagc
    50761 cgttttattg tgcggctctt tcgggctgat ataccagata cgcgcattct gcggcgtcat
    50821 catcgccaga cgttctttta ctgctttagc atcgtaccga tcggcaatat tgactgcatc
    50881 cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc agccattcga cgtaatccat
    50941 atcacgggtg atcgacggat aacggaagtc gatatccagc acattcgcca gttcatcgaa
    51001 gtattgttta tcaatgcctt tttcacgtaa cagattgaga tagctaaaaa ttgccgccac
    51061 aacctgatcg cgattagcca ggcctttatc ggttaaagac gcagagatcg ctaatacgcc
    51121 gctgttgccg ttgacgatag gatcggagtt ggcgctaatg ccctcaacta atccctgctt
    51181 ttgcagccag tcggaaagtg tacctgggct gcgattacca atcagatagg taatcaattc
    51241 atccgtttta ctacggaact tcgctgagtt gttatcgatg cgaaactcaa cgcgcagaac
    51301 tttacgcggc attgccggga cgtaatgaat gataatgccc ttttgcgcgt cggtgactac
    51361 cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc
    51421 cgccattttt gccaactccg gcagcggttt attgctgtaa ataaccgcct tcatcagatt
    51481 ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc tgcaccggat taccaggttt
    51541 atcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg ccgggttaat
    51601 ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc
    51661 agcgttcacc gcattacgct cacgttcggc atatttcttg tcgagcaaag gttcagcaat
    51721 agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg ttctcaactt ccagatagaa
    51781 agccgtgcga tacggcgcag tgctggcatt gtgactaccg ccgtgcattt tgagatattc
    51841 ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat gttcaaggta
    51901 atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca ccaccagcgc
    51961 cgagagcgat ttaactgcct gcggatcaga aaccagcaag accaccatac cgttatccag
    52021 acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt cctgaatcgg
    52081 ctgccatccc gtttctgcct gacttaaggg tgcccaaagg gcaactaaca acaataatgc
    52141 tttgaaccag gtgctgcggg gcattctcgg acctcataat caacttaatt ttctgtccag
    52201 attcaacacg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgca cgagtcagcc
    52261 tatgtttata taaccatcag tccgtgactg gtgcgcatca taaagtaagc ggatagattg
    52321 cgcaattttt atacagcact catgactgat taaagcgaaa cagcggtaac aggaaacgtt
    52381 gcgactgttc aacgatggcc tccattgtct ctggtgttaa ttgccgccag agcctttgat
    52441 accagatatc atcaccttcg ccacgcacca tcatgttacc ttcgtaagcc tgaaggaatt
    52501 tcgtacgggc tttttgcaac gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat
    52561 aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc gcggacatcc
    52621 cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg
    52681 gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg actttcacca ttaccaccgc
    52741 tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca cttaataaag
    52801 agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca gttatctgca
    52861 caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacag gcaatgactc
    52921 tgtcggcaag ctgctgcatc tcctggcact gagtttccca gaaaatttca ccaaaagcgc
    52981 catacggtaa atcccctgcc gcccggaagc gacggaacaa gcgttcggca tcatcctgct
    53041 caaccagtgc attcaataac tgctgattga tttgataacg gctaagccct tccagaataa
    53101 atggctcggt gtcggggatt tcgctgtctt cagtacggaa gttcacctgc aaacgcatct
    53161 ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag cgtttccagc ggcacggttt
    53221 ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca gcctggctgg
    53281 ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctgatag ttttgtggat
    53341 caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat
    53401 cacagttgag cgcttcatcg cccggtagat aatgactttg cccgatgtag tcgatcagtt
    53461 cctgcaccag taccgacggg aaacgttcac tgttatcctg aatggaacgc ccgatatagc
    53521 tgatatagag tttttgctgc gcggaaatta acgcttccag gaacagatag cggtcgtcat
    53581 cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag cccaatggcg
    53641 caagctgacg tggataaacg ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa
    53701 ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga
    53761 tacgttcttg atccagacgc tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt
    53821 cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt tcgatcagcg
    53881 tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc gctgagcata tcgcgacaaa
    53941 ccggcaacca ctcttctagc ggacgctcct gtgccagccc gcgacgccag atgtttagct
    54001 gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc gattcatcat
    54061 aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc agcaacatac
    54121 gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt
    54181 tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt aaataacgca
    54241 gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagt aatgccagca
    54301 cgtcttccga cacaaagcgg ctgtctggca acgataacag gctgataaac gcttccagta
    54361 ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag
    54421 gtgcgctacc aaacacagcc cgaataaacg gactgtagct gtcgatatca gccaccatca
    54481 cgatgatgtc gcgcggagta agtgtcgggt cttcttccag catcgccagc aggcgatcgt
    54541 gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg gaaggtgata ctgctatcca
    54601 gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttc acaccagcaa
    54661 cggcacggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg ttatctggcg
    54721 tgacatcgac aaaggcgtcc agctcctggc tgctctccag gtcagaaagg agataaatgt
    54781 agtcgcgccc aagcttaccc catgaagcca gcagcgggtt gccgacatcc tgttcaccat
    54841 cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa tagcggcaat tcgcgatctt
    54901 caaaactgtg tcgacgctga cgggtcagca gtttcgccag ataagcagga tctttaatat
    54961 cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata tgtttaccca
    55021 gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga aataccgcat ataaagacgc
    55081 gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata aagcgctgat
    55141 agagattggc gcggtgccag cgcggttgcc cgagttgatg ggtatattcc accagcgcct
    55201 tccacaacgg ggcttgccag gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt
    55261 cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg tccgccgctt
    55321 ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca
    55381 gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt catcagtttc cagctcatgc
    55441 tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg aacatatccc
    55501 agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aatactaaac ttttgcgaca
    55561 gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatc tctggttcga
    55621 aaggatcgtc cagccgttcg cgttcgacaa taaactccat caacgcttcc agcacgtcca
    55681 gacgattgga atggtagacc cttaacatag cggctcctga ctactgacga ttcgggcaat
    55741 gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga
    55801 cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg
    55861 cgcgcagttg cgtttgctgc caaccatgcc gccagagctg ttggtactgg tttctgctgg
    55921 cgagactgtt cattaatgtt cgctggatac ccgataacgc agtgacaatc atcaccatca
    55981 gcaccatcgc aaccattacc tccggcaggc taaagccttg ttgattcttc agggaagctg
    56041 acataacgcc ctctctttca gcggacaaaa atcgctccag ccgcgtggcg aaaaaacaat
    56101 gtttccatca atgacttcgc ctgttcgcca caacgaaacg ccttcataac cggcaaccaa
    56161 taaggcttca ttatctgcca gtaaacgcaa acaaacccgg gcatcggttc cagcgtactg
    56221 cgagcactga actgcgggct gcgtctgcca ggagtgcatt ttcccccacg ccagcgccga
    56281 ctgaacgatg gcctggcggc gcaatgactg actttctatg ctcacgcgag aggcaaaact
    56341 gcgatcctgc tgactcattc cttgcaatag caagctaccc aaaaccagca gcatcaggac
    56401 cagagccagt gacgaaacac ctttttcgcg gttcacaggt tgaatcctgt cacgctatag
    56461 ctggcatcca ccacggtttg cggttcagac ttactggcag cacgcatatt aaccgtcaac
    56521 accggcgaga agccgctgac atcctgacgt acgacctgaa aagtgtcgat aatgatggca
    56581 tccggattag tgactttatc ccagccctta ccttcacagg atgtcgcacc gcgtagcgtt
    56641 tccagcacat gctccttcag acgaaatcca atctggtcag actcttttac cggttcgcga
    56701 tcccagatac cgttactgtt cgcatcccac tgcacaatga tacagtcacc ctgtccgaca
    56761 atttccagcc cttcgccggt acagctgcca tgacaataac ccgccctctg gagatgcttc
    56821 gcgacggtaa atacccgcag ccagatttca tcttccagcg ccagcttacg ggtgctcgtt
    56881 aaactttcac gctgtaacgc aggcagaaag cgtgcagccc ccagcaacaa tacgctactg
    56941 atcgccatag caatcaacac ttccagcaga gaaaaacctt gctcttttac aggcatcctt
    57001 ctgtttctcc ttgctgacaa agccggagtc ttccccacgg cgaaaccacc agccaccact
    57061 cgcccgttga gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc aggccaaaga
    57121 aagcaagcga aggtgtcagg tcgctcattt cgacttcggg ccagcgtggc acaaagacca
    57181 atggtgaact gccatgacag gtattggccc cagcagcgga actcacaagg caccataacg
    57241 tcccctccct gataacgctg atactgtggt cgcggttatg ccagttggca tcttcacgta
    57301 aatagagcaa atagtcccgc gcctggctgg cggtttgcca tagccgttgc gactgctgcc
    57361 agtattgcca gccatagagt ccacttgcgc ttagcatgac caaaatcagc atcgcgacca
    57421 gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg agcgaggaga
    57481 aaaaaagacg agggccagtt tctatttctt cggcgcatct tccggactat ttacgccgtt
    57541 gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat ttaacggagg gtaaaaaaaa
    57601 ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct
    57661 ttaatgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag
    57721 atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat
    57781 tgcagatgag tttgatccat atgattgctg tacaggtgcg tatcgccacc ggtccagaca
    57841 aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg
    57901 gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag
    57961 agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta
    58021 tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gggaatccgg gtcgtttttc
    58081 agctggttca gtaccgtagt gatctggtca atatgacgac catctggcgt tggccaggcg
    58141 cgccactgtt taccatacac tggtccgagg tcgccgtttt catcggccca ttcgtcccag
    58201 atggtgacat tgttttcgtg tagataagca atgttagtgt cgccctgcag aaaccacagc
    58261 agttcgtgga tgatggaacg caggtggcaa cgtttagttg tcaccagcgg gaatccatcc
    58321 tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg
    58381 tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt
    58441 cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc
    58501 gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg
    58561 gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca
    58621 atcaggaaca agccagagac agctcccatt gggcgcggtt tacgaatata caggttgagg
    58681 ataataaaca gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg gtggcgcggc
    58741 agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata
    58801 tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc
    58861 cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt
    58921 ggtgcgataa aatcagaaac ctggaagaag gaacgtttag tacggcgggc aaagataatc
    58981 atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg
    59041 aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata
    59101 cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg
    59161 gtccagccgc tgcccggacg attcgcccgt cgtgttgcca gccacattgc aaaaatgaaa
    59221 cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc
    59281 ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta
    59341 tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt
    59401 gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat
    59461 gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct
    59521 gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt
    59581 tctgcttcgg cataatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg
    59641 ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc
    59701 gccatttcac cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg ggcgatcatc
    59761 gccagagctc gtaacattgc aggatgaaga ctgtcataaa tgttcgccac ccgggtattg
    59821 ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg
    59881 acccgctttg ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg
    59941 atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca
    60001 atcaggcggc gtgcttcatc aacttcatcg aggcttgtga ccatcggcaa cagaatattc
    60061 aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc
    60121 ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc
    60181 ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta
    60241 tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa
    60301 ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacggctg
    60361 cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca
    60421 ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc
    60481 tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat
    60541 tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc
    60601 gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct
    60661 gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc
    60721 gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta
    60781 gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcgcc agcccgctct
    60841 ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt
    60901 accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg
    60961 gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg
    61021 gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc
    61081 agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa
    61141 ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg
    61201 cggattcgcg tctggcgata ttgcccaaac aaggcagtca actgcgactg agaaagaata
    61261 gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc
    61321 aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg
    61381 cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc
    61441 ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct
    61501 tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc
    61561 aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc
    61621 gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct
    61681 accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta
    61741 cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca
    61801 ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg
    61861 accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc
    61921 atattgattt ctgcatcgcc gctcaccagc tgcaagagaa accatttttg tttttggccg
    61981 atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag
    62041 ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat
    62101 tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc
    62161 caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg
    62221 atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct
    62281 ttatatacga atgattgttt catactccaa gaagacggta aaccactctc tgcagggcat
    62341 tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg
    62401 ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt
    62461 actacgggaa aacccggagc attctgaata agcccgatat agacatttta aaatattcat
    62521 tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt
    62581 cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg
    62641 tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt
    62701 aaatggaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt
    62761 gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac
    62821 tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa
    62881 aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg
    62941 acatgtccca acctcgccca ctcctctctc ctcccgaaac tgaagaacag ttgttagcgc
    63001 aagcacagca actttctggt tatacattgg gagaactggc ggcacttgcc gggctggtta
    63061 cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag
    63121 gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa
    63181 ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt
    63241 taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg
    63301 tactgtggat accggttgaa ggtgagcgca gcatcccgct ggcgcagcgt cgcgtaggat
    63361 caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag
    63421 aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggagt
    63481 atttacagat acgaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc
    63541 ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg
    63601 cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg
    63661 catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac
    63721 cgatcctaac gcctggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat
    63781 cgacaatatt attttccttt ctctggtggt ggcaaagctc cccacagcac aacgtgctca
    63841 tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat
    63901 cgcctgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttccgc
    63961 ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat
    64021 ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg
    64081 cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc
    64141 tgtgggtctg tcagatcacc tgtttattat gatggccgcc gtggtgattg ccgtaggcgt
    64201 gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat
    64261 gctggcgctc tctttcctga ttctggtcgg ctttaccctg attctggaaa gtttcgacat
    64321 ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct
    64381 caacctgatt cgcaacaaaa agaacccgct ctgatactcc gtacgctctc ctgcgggagg
    64441 gcgtactcac tcctgttacc ctcacagttt aagattttcc tgctttcaag atatataacg
    64501 tcggtttata aacagactat tatcataggt gggccaaatg aggataaccg gatgaaaaaa
    64561 tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat
    64621 tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat
    64681 gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt
    64741 gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctgtaatgcc
    64801 gatcagttaa ggatca
//
LOCUS       DAJVXP010000007        60961 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVXP010000007 DAJVXP010000000
VERSION     DAJVXP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.007"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..468
                     /locus_tag="OQS10_000457"
     CDS             <1..468
                     /locus_tag="OQS10_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2291584.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="OQS10_000458"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="OQS10_000458"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS2291585.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="OQS10_000459"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="OQS10_000459"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS2291586.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OQS10_000460"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OQS10_000460"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS2291587.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="OQS10_000461"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="OQS10_000461"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS2291588.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="OQS10_000462"
     CDS             complement(4084..5313)
                     /locus_tag="OQS10_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS2291589.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="OQS10_000463"
     CDS             complement(5377..5817)
                     /locus_tag="OQS10_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS2291590.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="OQS10_000464"
     CDS             complement(5922..6692)
                     /locus_tag="OQS10_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2291591.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="OQS10_000465"
     CDS             complement(6689..7615)
                     /locus_tag="OQS10_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2291592.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="OQS10_000466"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="OQS10_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS2291593.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OQS10_000467"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OQS10_000467"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS2291594.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="OQS10_000468"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="OQS10_000468"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS2291595.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDMWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OQS10_000469"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OQS10_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS2291596.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="OQS10_000470"
     CDS             13322..13669
                     /locus_tag="OQS10_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS2291597.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="OQS10_000471"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="OQS10_000471"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS2291598.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="OQS10_000472"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="OQS10_000472"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS2291599.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="OQS10_000473"
     CDS             complement(15489..15851)
                     /locus_tag="OQS10_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS2291600.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OQS10_000474"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OQS10_000474"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS2291601.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="OQS10_000475"
     CDS             18978..20735
                     /locus_tag="OQS10_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS2291602.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OQS10_000476"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OQS10_000476"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS2291603.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OQS10_000477"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OQS10_000477"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS2291604.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OQS10_000478"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OQS10_000478"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS2291605.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OQS10_000479"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OQS10_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS2291606.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="OQS10_000480"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="OQS10_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS2291607.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OQS10_000481"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OQS10_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS2291608.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OQS10_000482"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OQS10_000482"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS2291609.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="OQS10_000483"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="OQS10_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS2291610.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="OQS10_000484"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="OQS10_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS2291611.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="OQS10_000485"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="OQS10_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="OQS10_000486"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="OQS10_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OQS10_000487"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OQS10_000487"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS2291612.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="OQS10_000488"
     CDS             complement(36855..36899)
                     /locus_tag="OQS10_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291613.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="OQS10_000489"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="OQS10_000489"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS2291614.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="OQS10_000490"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="OQS10_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS2291615.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="OQS10_000491"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="OQS10_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS2291616.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OQS10_000492"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OQS10_000492"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS2291617.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OQS10_000493"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OQS10_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS2291618.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OQS10_000494"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OQS10_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS2291619.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OQS10_000495"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OQS10_000495"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS2291620.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OQS10_000496"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OQS10_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS2291621.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OQS10_000497"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OQS10_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS2291622.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OQS10_000498"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OQS10_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS2291623.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OQS10_000499"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OQS10_000499"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS2291624.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OQS10_000500"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OQS10_000500"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS2291625.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OQS10_000501"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OQS10_000501"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS2291626.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OQS10_000502"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OQS10_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS2291627.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OQS10_000503"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OQS10_000503"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS2291628.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OQS10_000504"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OQS10_000504"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS2291629.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OQS10_000505"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OQS10_000505"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS2291630.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OQS10_000506"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OQS10_000506"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS2291631.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OQS10_000507"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OQS10_000507"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS2291632.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OQS10_000508"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OQS10_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS2291633.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OQS10_000509"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OQS10_000509"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS2291634.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OQS10_000510"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OQS10_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS2291635.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14597 a        16855 c        14999 g        14510 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacatg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacgcg
    60961 c
//
LOCUS       DAJVXP010000008        59824 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVXP010000008 DAJVXP010000000
VERSION     DAJVXP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.008"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..262)
                     /locus_tag="OQS10_000511"
     CDS             complement(<1..262)
                     /locus_tag="OQS10_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2291636.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OQS10_000512"
     CDS             369..665
                     /locus_tag="OQS10_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2291637.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OQS10_000513"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OQS10_000513"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS2291638.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OQS10_000514"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OQS10_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS2291639.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OQS10_000515"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OQS10_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS2291640.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OQS10_000516"
     CDS             complement(3472..3645)
                     /locus_tag="OQS10_000516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291641.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OQS10_000517"
     CDS             3673..3756
                     /locus_tag="OQS10_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS2291642.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OQS10_000518"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OQS10_000518"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS2291643.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OQS10_000519"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OQS10_000519"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS2291644.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OQS10_000520"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OQS10_000520"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS2291645.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OQS10_000521"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OQS10_000521"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS2291646.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OQS10_000522"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OQS10_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS2291647.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OQS10_000523"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OQS10_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS2291648.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OQS10_000524"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OQS10_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS2291649.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OQS10_000525"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OQS10_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS2291650.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OQS10_000526"
     CDS             14052..15254
                     /locus_tag="OQS10_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS2291651.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OQS10_000527"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OQS10_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OQS10_000528"
     CDS             16953..18560
                     /locus_tag="OQS10_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS2291652.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OQS10_000529"
     CDS             18822..20444
                     /locus_tag="OQS10_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS2291653.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OQS10_000530"
     CDS             20810..21877
                     /locus_tag="OQS10_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS2291654.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OQS10_000531"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OQS10_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS2291655.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OQS10_000532"
     CDS             24609..25733
                     /locus_tag="OQS10_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2291656.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OQS10_000533"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OQS10_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OQS10_000534"
     CDS             26076..26435
                     /locus_tag="OQS10_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS2291657.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OQS10_000535"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OQS10_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS2291658.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OQS10_000536"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OQS10_000536"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS2291659.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OQS10_000537"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OQS10_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS2291660.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OQS10_000538"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OQS10_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS2291661.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OQS10_000539"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OQS10_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS2291662.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OQS10_000540"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OQS10_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS2291663.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OQS10_000541"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OQS10_000541"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS2291664.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OQS10_000542"
     CDS             complement(32630..33679)
                     /locus_tag="OQS10_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS2291665.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OQS10_000543"
     CDS             33811..35028
                     /locus_tag="OQS10_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2291666.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OQS10_000544"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OQS10_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS2291667.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OQS10_000545"
     CDS             complement(35662..36327)
                     /locus_tag="OQS10_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS2291668.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OQS10_000546"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OQS10_000546"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS2291669.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OQS10_000547"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OQS10_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS2291670.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OQS10_000548"
     CDS             complement(39167..39793)
                     /locus_tag="OQS10_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS2291671.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OQS10_000549"
     CDS             39934..40293
                     /locus_tag="OQS10_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS2291672.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OQS10_000550"
     CDS             complement(40296..40565)
                     /locus_tag="OQS10_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS2291673.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OQS10_000551"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OQS10_000551"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS2291674.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OQS10_000552"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OQS10_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS2291675.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OQS10_000553"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OQS10_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS2291676.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OQS10_000554"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OQS10_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS2291677.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OQS10_000555"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OQS10_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS2291678.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OQS10_000556"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OQS10_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS2291679.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OQS10_000557"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OQS10_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS2291680.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OQS10_000558"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OQS10_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS2291681.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OQS10_000559"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OQS10_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS2291682.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OQS10_000560"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OQS10_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS2291683.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OQS10_000561"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OQS10_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS2291684.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OQS10_000562"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OQS10_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS2291685.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OQS10_000563"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OQS10_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OQS10_000564"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OQS10_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS2291686.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OQS10_000565"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OQS10_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS2291687.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57064
                     /gene="ugpC"
                     /locus_tag="OQS10_000566"
                     /pseudo
     CDS             56011..57064
                     /gene="ugpC"
                     /locus_tag="OQS10_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
     gene            57061..57804
                     /gene="ugpQ"
                     /locus_tag="OQS10_000567"
     CDS             57061..57804
                     /gene="ugpQ"
                     /locus_tag="OQS10_000567"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS2291688.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57791..58231)
                     /locus_tag="OQS10_000568"
     CDS             complement(57791..58231)
                     /locus_tag="OQS10_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS2291689.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58351..>59824
                     /gene="ggt"
                     /locus_tag="OQS10_000569"
     CDS             58351..>59824
                     /gene="ggt"
                     /locus_tag="OQS10_000569"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS2291690.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14591 a        15106 c        16134 g        13993 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgagtaat ggtgatgaac ggtggcgttg ccgaacagat
    56641 tggcacacca gttgaagtct acgaaaagcc cgccagcctg tttgtggcga gttttatcgg
    56701 cagcccggcg atgaacctgc tgacaggccg cgtgaataat gaaggcacgc atttcgaact
    56761 ggacggcggt atcgtgctac cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat
    56821 gactctcggc attcgcccgg aacatatagc gctaagctcg caggcagaag gtggtgtgcc
    56881 gctggtgatg gacacgctgg agatcctcgg cgcagataac ctggcgcacg gacgctgggg
    56941 cgagcagaag ctggtggtgc gactggcgca tcaggagcgc ccgacggcag gcagcacgct
    57001 gtggctgcat ttggcggaaa atcagctgca tctttttgat ggtgaaacag gacaacgagt
    57061 atgagtaact ggccttatcc ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg
    57121 gaaaacaccc tggcggcaat cgacgtcggg gcaaaatacg gtcataagat gatcgaattt
    57181 gacgcgaagt tatcgaaaga tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc
    57241 accagcaacg gctggggcgt cgcgggtgaa ctgaactggc aggatttact gcgcgtggat
    57301 gcgggcagtt ggtacagcaa agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg
    57361 gcggaacgct gtcgcgaaca cgggatgatg gcgaatatcg aaatcaaacc caccactggc
    57421 actggaccat taacgggcaa aatggtggcg ctggcggcac gccaactgtg ggccggtatg
    57481 acgccgccgc tgctgtcatc gtttgagatt gatgctttag aagctgcaca acaggcggca
    57541 ccggaactgc cgcgtggttt attgcttgat gagtggcgcg acgactggcg cgaactgacc
    57601 gcgcggctgg gctgcgtctc tattcatctc aatcataagt tactcgataa agcgcgagtg
    57661 atgcagttga aagacgccgg attacggatt ctggtttata ccgtcaataa accccagcgc
    57721 gcggcagagt tgctgcgctg gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt
    57781 ggtccgaact ttacggccca atagttttca acggaatgtc aggctgcggc gtgctgggct
    57841 gattcagcat gtcgccgttt ctcctttccg gcagcatatg ctgctgattc aacgaactat
    57901 ccggattacg gttgctgttt aacatcccgc cgttggtgtt gggcagcatt tgctgccggg
    57961 cgggatttcg ctcccccggc tgtgactgca acacccgctg agaattattg tttatctggt
    58021 tttctaaatg ctgctgttgc aactgagttt gcgttttcag ttgctgattc agcatccctt
    58081 tttgctggat ttgctgagtc tgcatctggg tttgcatccg ctgctggctg ggtatctgat
    58141 accccggctg gttagggttg ttcagagtat taatgggctg tgcaaagccg acaaacggca
    58201 ggagtgccgt aagaatcaga agtcgtttca tcgcgtatcc tcctctgaag atatccttta
    58261 agtttactcg cttcccgaca aaacgatgat taattcagag ttatatacca ggcttagctg
    58321 gggttgcccc ttaatctctg gagaataacg atgataaaac cgacgttttt acgccgggtg
    58381 gccattgctg ctctgctctc aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg
    58441 cccgtctcgt atggtgtgga ggaagatgtc ttccacccgg tacgcgcgaa acagggaatg
    58501 gtagcgtctg tggacgccac tgccactcag gtgggggtgg atatcctcaa ggagggcggg
    58561 aatgccgttg atgccgccgt ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca
    58621 gggaatctgg gcggtggtgg ttttatgtta atccgctcga aaaatggcaa taccacggct
    58681 atcgatttcc gcgaaatggc acccgccaaa gcgacccgcg atatgttcct cgatgatcag
    58741 ggcaacccgg acagcaaaaa atcactcact tcgcatctgg cttccggcac accgggtacg
    58801 gtagcaggtt tctcgctggc gctggataaa tacggcacca tgccgctgaa caaagtcgtg
    58861 cagcccgcgt ttaaactggc acgcgatggt tttatcgtta acgacgcgct ggctgacgat
    58921 ctcaaaacct acggtagcga agtgttgccg aatcacgaaa acagtaaagc tatcttctgg
    58981 aaagagggcg agccgctgaa aaagggcgac acgctggtgc aggcgaacct ggcaaagagc
    59041 ctggagatga ttgctgaaaa cggcccggac gaattctata aaggcacgat tgcggaacag
    59101 atcgcccagg agatgcagaa aaacggtggc ttgatcacta aagaagattt agcggcctat
    59161 aaagcggtcg aacgcactcc gataagcggc gattatcgcg ggtatcaggt ttactccatg
    59221 ccaccgccat cctccggcgg gatccatatc gtacaaatcc tcaatattct ggaaaacttc
    59281 gatatgaaga aatacggctt tggcagcgcc gatgcgatgc aaatcatggc agaagcggag
    59341 aaatacgcct acgccgaccg ctcggaatat cttggcgacc cggattttgt caaagtcccg
    59401 tggcaggcac tgaccaataa agcctatgcc aaatccattg ccgatcaaat tgatatcaac
    59461 aaagcgaagc cgtccagtga gattcgccct ggcaagcttg cgccttatga gagtaatcaa
    59521 actacccatt actctgtagt ggataaagac ggtaacgcgg tggcggtgac ctatacgctg
    59581 aacaccacct tcggtacggg cattgtcgcg ggcgagagcg gtattctgct taataatcag
    59641 atggatgatt tctccgccaa accgggcgta ccgaacattt acgggctggt gggcggtgat
    59701 gccaacgccg tcgggccgaa taaacgtccg ctgtcatcga tgtcaccaac cattgtggtg
    59761 aaagacggta aaacttggct ggttaccggt agtcctggcg ggagtcggat tattaccacc
    59821 gtgc
//
LOCUS       DAJVXP010000009        58385 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVXP010000009 DAJVXP010000000
VERSION     DAJVXP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.009"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..160
                     /locus_tag="OQS10_000570"
     CDS             <1..160
                     /locus_tag="OQS10_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS2291691.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="OQS10_000571"
     CDS             complement(198..1259)
                     /locus_tag="OQS10_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS2291692.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="OQS10_000572"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="OQS10_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS2291693.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="OQS10_000573"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="OQS10_000573"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS2291694.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OQS10_000574"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OQS10_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS2291695.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OQS10_000575"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OQS10_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS2291696.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OQS10_000576"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OQS10_000576"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS2291697.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OQS10_000577"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OQS10_000577"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS2291698.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OQS10_000578"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OQS10_000578"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS2291699.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OQS10_000579"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OQS10_000579"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS2291700.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OQS10_000580"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OQS10_000580"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS2291701.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OQS10_000581"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OQS10_000581"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS2291702.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="OQS10_000582"
     CDS             complement(14497..14649)
                     /locus_tag="OQS10_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291703.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="OQS10_000583"
     CDS             14667..14858
                     /locus_tag="OQS10_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS2291704.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="OQS10_000584"
     CDS             15169..15687
                     /locus_tag="OQS10_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS2291705.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="OQS10_000585"
     CDS             15703..16242
                     /locus_tag="OQS10_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS2291706.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OQS10_000586"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OQS10_000586"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS2291707.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OQS10_000587"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OQS10_000587"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS2291708.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="OQS10_000588"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="OQS10_000588"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS2291709.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="OQS10_000589"
     CDS             complement(20978..21193)
                     /locus_tag="OQS10_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS2291710.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="OQS10_000590"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="OQS10_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS2291711.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OQS10_000591"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OQS10_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS2291712.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OQS10_000592"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OQS10_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS2291713.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OQS10_000593"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OQS10_000593"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS2291714.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OQS10_000594"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OQS10_000594"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS2291715.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OQS10_000595"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OQS10_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS2291716.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OQS10_000596"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OQS10_000596"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS2291717.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OQS10_000597"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OQS10_000597"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS2291718.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OQS10_000598"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OQS10_000598"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS2291719.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OQS10_000599"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OQS10_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS2291720.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="OQS10_000600"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="OQS10_000600"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS2291721.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OQS10_000601"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OQS10_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS2291722.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESAVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OQS10_000602"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OQS10_000602"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS2291723.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OQS10_000603"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OQS10_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS2291724.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OQS10_000604"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OQS10_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS2291725.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OQS10_000605"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OQS10_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS2291726.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OQS10_000606"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OQS10_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS2291727.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OQS10_000607"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OQS10_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS2291728.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OQS10_000608"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OQS10_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS2291729.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OQS10_000609"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OQS10_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS2291730.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OQS10_000610"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OQS10_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS2291731.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OQS10_000611"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OQS10_000611"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS2291732.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="OQS10_000612"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="OQS10_000612"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS2291733.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="OQS10_000613"
     CDS             49418..50272
                     /locus_tag="OQS10_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2291734.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="OQS10_000614"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="OQS10_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS2291735.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="OQS10_000615"
     CDS             complement(51735..52874)
                     /locus_tag="OQS10_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS2291736.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OQS10_000616"
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OQS10_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS2291737.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54060..55421
                     /locus_tag="OQS10_000617"
     CDS             54060..55421
                     /locus_tag="OQS10_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS2291738.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="OQS10_000618"
     CDS             55418..55936
                     /locus_tag="OQS10_000618"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS2291739.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="OQS10_000619"
     CDS             55936..56256
                     /locus_tag="OQS10_000619"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS2291740.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="OQS10_000620"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="OQS10_000620"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS2291741.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..58277
                     /locus_tag="OQS10_000621"
     CDS             57075..58277
                     /locus_tag="OQS10_000621"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS2291742.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14271 a        15785 c        14566 g        13763 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggc ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag aattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacacgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccaaca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    58081 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    58141 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    58201 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    58261 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    58321 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    58381 cacta
//
LOCUS       DAJVXP010000010        57431 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVXP010000010 DAJVXP010000000
VERSION     DAJVXP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.010"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="OQS10_000622"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="OQS10_000622"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS2291743.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3103)
                     /locus_tag="OQS10_000623"
     CDS             complement(1496..3103)
                     /locus_tag="OQS10_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS2291744.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3581..3667)
                     /locus_tag="OQS10_000624"
     tRNA            complement(3581..3667)
                     /locus_tag="OQS10_000624"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OQS10_000625"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="OQS10_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS2291745.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OQS10_000626"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="OQS10_000626"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS2291746.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OQS10_000627"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="OQS10_000627"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS2291747.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OQS10_000628"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="OQS10_000628"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS2291748.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OQS10_000629"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="OQS10_000629"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS2291749.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="OQS10_000630"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="OQS10_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS2291750.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OQS10_000631"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="OQS10_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS2291751.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="OQS10_000632"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="OQS10_000632"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS2291752.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OQS10_000633"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="OQS10_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS2291753.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="OQS10_000634"
     CDS             complement(13279..14244)
                     /locus_tag="OQS10_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS2291754.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OQS10_000635"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="OQS10_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS2291755.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OQS10_000636"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="OQS10_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS2291756.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="OQS10_000637"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="OQS10_000637"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS2291757.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="OQS10_000638"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="OQS10_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS2291758.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="OQS10_000639"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="OQS10_000639"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS2291759.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="OQS10_000640"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="OQS10_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS2291760.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="OQS10_000641"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="OQS10_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS2291761.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="OQS10_000642"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="OQS10_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS2291762.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="OQS10_000643"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="OQS10_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS2291763.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="OQS10_000644"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="OQS10_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS2291764.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="OQS10_000645"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="OQS10_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS2291765.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="OQS10_000646"
     CDS             21785..22762
                     /locus_tag="OQS10_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS2291766.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="OQS10_000647"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="OQS10_000647"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS2291767.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="OQS10_000648"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="OQS10_000648"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS2291768.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="OQS10_000649"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="OQS10_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS2291769.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="OQS10_000650"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="OQS10_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS2291770.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="OQS10_000651"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="OQS10_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS2291771.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="OQS10_000652"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="OQS10_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS2291772.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="OQS10_000653"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="OQS10_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS2291773.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="OQS10_000654"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="OQS10_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS2291774.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="OQS10_000655"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="OQS10_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS2291775.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="OQS10_000656"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="OQS10_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS2291776.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="OQS10_000657"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="OQS10_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS2291777.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="OQS10_000658"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="OQS10_000658"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS2291778.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="OQS10_000659"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="OQS10_000659"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS2291779.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="OQS10_000660"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="OQS10_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS2291780.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="OQS10_000661"
     CDS             complement(34631..35560)
                     /locus_tag="OQS10_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS2291781.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="OQS10_000662"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="OQS10_000662"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS2291782.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="OQS10_000663"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="OQS10_000663"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS2291783.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42789
                     /locus_tag="OQS10_000664"
     CDS             42310..42789
                     /locus_tag="OQS10_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS2291784.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42806..43111
                     /locus_tag="OQS10_000665"
     CDS             42806..43111
                     /locus_tag="OQS10_000665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2291785.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /locus_tag="OQS10_000666"
     CDS             complement(43187..43651)
                     /locus_tag="OQS10_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS2291786.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="OQS10_000667"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="OQS10_000667"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS2291787.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="OQS10_000668"
     CDS             complement(44520..45209)
                     /locus_tag="OQS10_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS2291788.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="OQS10_000669"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="OQS10_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS2291789.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="OQS10_000670"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="OQS10_000670"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS2291790.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="OQS10_000671"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="OQS10_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS2291791.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="OQS10_000672"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="OQS10_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS2291792.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="OQS10_000673"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="OQS10_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS2291793.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="OQS10_000674"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="OQS10_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS2291794.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="OQS10_000675"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="OQS10_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS2291795.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="OQS10_000676"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="OQS10_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS2291796.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="OQS10_000677"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="OQS10_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS2291797.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /locus_tag="OQS10_000678"
     CDS             54320..54718
                     /locus_tag="OQS10_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS2291798.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="OQS10_000679"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="OQS10_000679"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS2291799.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="OQS10_000680"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="OQS10_000680"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS2291800.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14263 a        15303 c        14255 g        13610 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg ataaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtctacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccatt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatgaccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcattttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggtg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttgg
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc aaactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcagaaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcaacaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacaca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagtt ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg c
//
LOCUS       DAJVXP010000011        48739 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVXP010000011 DAJVXP010000000
VERSION     DAJVXP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.011"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..562
                     /locus_tag="OQS10_000681"
     CDS             <1..562
                     /locus_tag="OQS10_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCS2291801.1"
                     /translation="DRIKAINEIRMAIHQVSPFREEPVDCVLWVKNSQLMPNDYNPNN
                     VAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYL
                     PVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDE
                     VLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(563..1723)
                     /gene="ybdL"
                     /locus_tag="OQS10_000682"
     CDS             complement(563..1723)
                     /gene="ybdL"
                     /locus_tag="OQS10_000682"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCS2291802.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1832..2920
                     /gene="hcxA"
                     /locus_tag="OQS10_000683"
     CDS             1832..2920
                     /gene="hcxA"
                     /locus_tag="OQS10_000683"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS2291803.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2930..3127)
                     /locus_tag="OQS10_000684"
     CDS             complement(2930..3127)
                     /locus_tag="OQS10_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS2291804.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3309..5414)
                     /gene="cstA"
                     /locus_tag="OQS10_000685"
     CDS             complement(3309..5414)
                     /gene="cstA"
                     /locus_tag="OQS10_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS2291805.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5595..6008)
                     /gene="entH"
                     /locus_tag="OQS10_000686"
     CDS             complement(5595..6008)
                     /gene="entH"
                     /locus_tag="OQS10_000686"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS2291806.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6011..6757)
                     /gene="entA"
                     /locus_tag="OQS10_000687"
     CDS             complement(6011..6757)
                     /gene="entA"
                     /locus_tag="OQS10_000687"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS2291807.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6757..7614)
                     /gene="entB"
                     /locus_tag="OQS10_000688"
     CDS             complement(6757..7614)
                     /gene="entB"
                     /locus_tag="OQS10_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS2291808.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7628..9238)
                     /gene="entE"
                     /locus_tag="OQS10_000689"
     CDS             complement(7628..9238)
                     /gene="entE"
                     /locus_tag="OQS10_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS2291809.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9248..10423)
                     /gene="entC"
                     /locus_tag="OQS10_000690"
     CDS             complement(9248..10423)
                     /gene="entC"
                     /locus_tag="OQS10_000690"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS2291810.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10798..11763
                     /gene="fepB"
                     /locus_tag="OQS10_000691"
     CDS             10798..11763
                     /gene="fepB"
                     /locus_tag="OQS10_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS2291811.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11844..13094)
                     /gene="entS"
                     /locus_tag="OQS10_000692"
     CDS             complement(11844..13094)
                     /gene="entS"
                     /locus_tag="OQS10_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS2291812.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13205..14209
                     /gene="fepD"
                     /locus_tag="OQS10_000693"
     CDS             13205..14209
                     /gene="fepD"
                     /locus_tag="OQS10_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS2291813.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14206..15198
                     /gene="fepG"
                     /locus_tag="OQS10_000694"
     CDS             14206..15198
                     /gene="fepG"
                     /locus_tag="OQS10_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS2291814.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15195..16010
                     /gene="fepC"
                     /locus_tag="OQS10_000695"
     CDS             15195..16010
                     /gene="fepC"
                     /locus_tag="OQS10_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS2291815.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16007..17140)
                     /gene="fepE"
                     /locus_tag="OQS10_000696"
     CDS             complement(16007..17140)
                     /gene="fepE"
                     /locus_tag="OQS10_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS2291816.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17356..21237)
                     /gene="entF"
                     /locus_tag="OQS10_000697"
     CDS             complement(17356..21237)
                     /gene="entF"
                     /locus_tag="OQS10_000697"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS2291817.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21234..21452)
                     /gene="ybdZ"
                     /locus_tag="OQS10_000698"
     CDS             complement(21234..21452)
                     /gene="ybdZ"
                     /locus_tag="OQS10_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS2291818.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21455..22657)
                     /gene="fes"
                     /locus_tag="OQS10_000699"
     CDS             complement(21455..22657)
                     /gene="fes"
                     /locus_tag="OQS10_000699"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS2291819.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            22900..25140
                     /gene="fepA"
                     /locus_tag="OQS10_000700"
     CDS             22900..25140
                     /gene="fepA"
                     /locus_tag="OQS10_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS2291820.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25315..25935
                     /gene="entD"
                     /locus_tag="OQS10_000701"
     CDS             25315..25935
                     /gene="entD"
                     /locus_tag="OQS10_000701"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS2291821.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26057..26209)
                     /gene="hokE"
                     /locus_tag="OQS10_000702"
     CDS             complement(26057..26209)
                     /gene="hokE"
                     /locus_tag="OQS10_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS2291822.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26560..26712)
                     /gene="hokE"
                     /locus_tag="OQS10_000703"
     CDS             complement(26560..26712)
                     /gene="hokE"
                     /locus_tag="OQS10_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS2291823.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27152..28270
                     /locus_tag="OQS10_000704"
     CDS             27152..28270
                     /locus_tag="OQS10_000704"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS2291824.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            28336..28584
                     /locus_tag="OQS10_000705"
                     /pseudo
     CDS             28336..28584
                     /locus_tag="OQS10_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            28649..29017
                     /locus_tag="OQS10_000706"
     CDS             28649..29017
                     /locus_tag="OQS10_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS2291825.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29111..29764
                     /gene="nfsB"
                     /locus_tag="OQS10_000707"
     CDS             29111..29764
                     /gene="nfsB"
                     /locus_tag="OQS10_000707"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS2291826.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            29872..31119
                     /gene="ybdG"
                     /locus_tag="OQS10_000708"
     CDS             29872..31119
                     /gene="ybdG"
                     /locus_tag="OQS10_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS2291827.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(31187..32563)
                     /gene="pheP"
                     /locus_tag="OQS10_000709"
     CDS             complement(31187..32563)
                     /gene="pheP"
                     /locus_tag="OQS10_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS2291828.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(32665..35808)
                     /gene="cusA"
                     /locus_tag="OQS10_000710"
     CDS             complement(32665..35808)
                     /gene="cusA"
                     /locus_tag="OQS10_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS2291829.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(35820..37043)
                     /gene="cusB"
                     /locus_tag="OQS10_000711"
     CDS             complement(35820..37043)
                     /gene="cusB"
                     /locus_tag="OQS10_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS2291830.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(37059..37391)
                     /gene="cusF"
                     /locus_tag="OQS10_000712"
     CDS             complement(37059..37391)
                     /gene="cusF"
                     /locus_tag="OQS10_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS2291831.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(37415..38797)
                     /gene="cusC"
                     /locus_tag="OQS10_000713"
     CDS             complement(37415..38797)
                     /gene="cusC"
                     /locus_tag="OQS10_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS2291832.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            38954..39637
                     /gene="cusR"
                     /locus_tag="OQS10_000714"
     CDS             38954..39637
                     /gene="cusR"
                     /locus_tag="OQS10_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS2291833.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            39627..41075
                     /gene="cusS"
                     /locus_tag="OQS10_000715"
     CDS             39627..41075
                     /gene="cusS"
                     /locus_tag="OQS10_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS2291834.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            41812..43713
                     /locus_tag="OQS10_000716"
     CDS             41812..43713
                     /locus_tag="OQS10_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2291835.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            43741..44202
                     /locus_tag="OQS10_000717"
     CDS             43741..44202
                     /locus_tag="OQS10_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCS2291836.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            44222..48633
                     /locus_tag="OQS10_000718"
                     /pseudo
     CDS             44222..48633
                     /locus_tag="OQS10_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        11655 a        13434 c        12792 g        10858 t
ORIGIN      
        1 tgatcgcatc aaagcgatta atgaaatccg catggcgatc catcaggtta gcccctttcg
       61 tgaagagcca gtggattgcg ttctgtgggt taaaaacagc caacttatgc ctaatgatta
      121 caacccaaac aacgtggcac cacccgaaaa aaaactgctg caaaaatcca ttgaaataga
      181 tggttttacc caacccattg tggtcacaca tacggataaa aatgccatgg aaatcgtgga
      241 tggttttcac cgccatgaaa ttggtaaagg ctcatcatca ttaaaattac gcttaaaagg
      301 ttatttgccg gtgacctgtc tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg
      361 tcataatcgc gctcgtggac gccatcaaat caccgccatg tcggaaatag tccgtgaact
      421 tagccagttg ggatgggacg ataataaaat tggcaaagag ctgggaatgg acagcgacga
      481 agtgctgcgc ctgaagcaaa ttaacggcct gcaagagctg tttgccgacc gtcaatattc
      541 ccgcgcctgg acggttaaat aactaaagct ggcgcaggcg ttcagctgct gccagcaacg
      601 tcgattcctt cttggcaaaa cagagacgaa tcagtttatg tgggaaggga tcggcgcaaa
      661 acaccgacag cggaatcgcc gctacgccgt gctcctgcgt cagccactgg caaaactcaa
      721 catcatccag ggtagaaacc gcgctgtaat ccaccagcaa aaagtatgta ccttcacacg
      781 gtaaaatctc cagccggctt tcatttaagg catttaccag aatatcgcgc ttctggcgat
      841 aaaagtccgg taacgcaaga taatgctcag gttctgcacg tagcatatca gcaagcgcca
      901 gctgtgccgg ggtattcacc gaaaaggtca gatactgatg taccttgcga atttcggcgc
      961 tgatgggcgc tggcgcaaca caataaccca ctttccagcc ggtcatatga taggtcttgc
     1021 caaatgaaga aaccgccact gcccgctcac gcagctgcga atgcgccagc acactggcat
     1081 ggccctgttg tgaaaagttg atgtgctcgt agacttcatc gctaatgaca aaaatctcgt
     1141 gcccggcgat cgcctgccac aaagcggcga aatcagcctg ctgccagaca gttgcactgg
     1201 agttatgcgg agtgttgagg atcaccagtc tggtgcgctc gcttaataat gcggcaaatt
     1261 cctgccagtc aacgcgaaaa tgcggtggtt gcagtgccat acgcttcact attcccccag
     1321 aaagcgcgat ggcgggggcg taactgtcat agctgggatc aaaacaaatc acttcatcgc
     1381 cattgcgcac cagtgcggta atcgccgcgt ataacgcttc cgtcgcccct gccgttacgg
     1441 tgatatcgct atcggcatct ggttgatagc catacaaacg ttccgttttc tgagcaatcg
     1501 cctcgcgcaa ggcctgcacg ccggtcatgg gcgcgtattg gtttgccccc tgggcaacgt
     1561 ggtacgccag ccgctcctgt aaatagcgcg gaccatcaaa atcaggaaag ccttgcgaca
     1621 ggttaatcgc ctggtgttgc tgcgccagcg cgctcatctg ggtgaaaata gtggtgccaa
     1681 gttgtggaag tttgctttgt ggaatcagag ggttatttgt catttttatt gtacctgacg
     1741 aagttgcgtg ttgaaggcac tataacacga tgctagtatt tggcaatcaa gacgtttaga
     1801 tgtctaaata aaacaataag gacaacacaa catgcctcac aatcctatcc gcgtggtcgt
     1861 cggcccggct aactactttt cacatccagg aagtttcaat cacctgaacg attttttcac
     1921 tgatgaacaa ctttctcgcg cggtgtggat ctacggcaaa cgcgccattg ctgcggcgca
     1981 aaccaaactt ccgccagcgt ttggacttcc aggggcaaag catattttgt ttcgtggtca
     2041 ttgcagcgaa agcgatgtac agcaactggc ggctgagtcc ggtgacgatc gcagcgtggt
     2101 gattggcgtc ggcggcggcg cactgctcga caccgcgaaa gccctcgccc gccgtctcgg
     2161 tctgccattt gttgccgttc cgacgatcgc cgctacctgc gccgcctgga caccgctctc
     2221 cgtctggtat aacgatgccg gacaggcgct gcattatgag attttcgacg acgccaattt
     2281 tatggtgctg gtggaaccag agattatcct caacgcgccg caacaatatc tgctggcggg
     2341 gatcggtgac acgctggcga aatggtatga agcggtggtg ctggctccgc aaccagaaac
     2401 gttgccgcta accgtgcgac tggggatcaa taatgcgcag gccattcgcg acgtattgtt
     2461 aaacagtagc gaacaggcgc tgagcgatca gcaaaatcaa cagttaacgc aatcattttg
     2521 cgatgtggtg gatgctatta ttgctggtgg tgggatggtc ggtgatctgg gcgatcgttt
     2581 tacgcgtgtg gcggcggctc atgccgtgca taacggtctg actgtcctgc cgcaaaccga
     2641 gaaatttctc cacggcacca aagtcgctta tggcattctg gtacaaagcg ccttgctagg
     2701 tcaggatgat gtgctggcgc aattaaccgg ggcgtatcag cgttttcatc tgccgaccac
     2761 gctggcggag ctggaaatgg atatcaataa tcatgcggag atcgacaaag tgattaccca
     2821 caccctgcgt ccggtggaat acattcatta cctgccagtc acgctgacac cagatacgtt
     2881 gcgtgcagcg ttcgaaaaag tggaatcgtt taaagcctga cgtgcaaatt tagcagcagc
     2941 gcgcgccgcc ttttccaccg tagcgcgcgt cctgccgctc acggaaaaac tcttcgtagg
     3001 tcatcggcgt ttgatcggga tggttaaccc gcatatgttc gacatagttg tcgtaatcag
     3061 gcataccaat catcagcttc gccgcctgac ctaaatattt tccggctttt gccagtgaat
     3121 caaacatctt ggcctccgct aaccatgttg ctgaaagagt gccggatgcg gcgtgaacgc
     3181 cttatccggc ctacaaatgc tatcaacgcg ctcggtcagt cccctcgccc catcggggag
     3241 agggttaggg tgaggaggaa ccctcggcac tatgccaaca ttcgccaaca tcccccctca
     3301 ctctgacttt agtgtgcgcc ttttgcctgc gccacgatct cctcgacatt ttccggcatt
     3361 ggctcatacg gcgtttcttt cgccgttggc ttcggatctt tcaatgccgc cagtgccgac
     3421 ttaatcgaga acagcgccag aaccaccacg accaccataa agaagatggt taacccggca
     3481 tccagacggt tgttgaacac cagttgcgcc agctgtgact cagtatactg cgacggaata
     3541 ttgccgctgt ctatcattgc ctggaactta ttagcaatgg ccaggaagcc gactttcgca
     3601 tccgggctaa acgctttctg ccagcctgcg gtcagggtac aaatcagcag ccaggccgtt
     3661 ggtaccagcg ccacccaggc gtaacgttga cgtttcatct tgaacaacac cacggcacag
     3721 agcatcagcg ccatccctgc cagcatctgg ttggcaatac caaacagcgg ccacagagtg
     3781 ttaatgccgc ccagcggatc gaccacgccc tgatggagga agtagcccca cgccagtacg
     3841 cacagcgctg ttgccagcag gttagcaggc agtgaatcgg tccgcttcag gccaggagac
     3901 accacgccca gcagatcctg caacataaag cgcgcagcac gcgtacccgc atctaccgcc
     3961 gtcagaataa acagagcttc aaacagaatg gcgaagtgat accagaacgc cacatccatc
     4021 ataccaccca gcgcaccgtg caggatgtaa gccatcccca ccgccagcgt cggcgcaccg
     4081 cccgcacggg aaatgatcga ctgttcaccc acttcgctgg caatctggtt gagcgtatcc
     4141 ggagtaatcg caaagcccca gctactcacc acctgcgcgg cagaagcgac cacatccgcc
     4201 gtccctgccg gagccagcac cgccatcggg ctgttcatgg cgaagtacac gcccggatcg
     4261 atgatacagg cggaaaccag tgccataatc gccacgaagg attccattaa cattccaccg
     4321 tagccgataa agcacgcctg cccttcgttc gccagcatct tcggcgtggt gccggaagag
     4381 atcagcgcat ggaagcctga caccgcgcca caggcgatgg tgataaacag gaacgggaac
     4441 agattaccgg tccataccgg gccagtgcca tcgacaaatt tggtcagcgc aggcatggtc
     4501 agcgtcggac gcataatcaa aatgcctacc gccagaccaa cgatagtccc gattttcagg
     4561 aaggtagaga ggtagtcacg cggggccagc agtaaccaca ccggcagcac cgccgccaca
     4621 aaaccgtagc ccaccagcat ccaggtcagc tgcacgccgg taaagtcaaa gtacggtgcc
     4681 caggtcgggc tttctgccac ccagccgcca gagataatgg caaaaataag gaacaccagg
     4741 ccaatcactg acacttcgcc aatacgcccc ggacgcagat agcgcaggta gattcccata
     4801 aaaatcgcca gcggaatggt gaacgcaacg gtatacgttc cccacgggct atgagtcagg
     4861 gctttcacca cgatcatcgc cagtactgca aggataatga ccatgatcat aaagcaggct
     4921 accagcgcaa tcaccccggc agttggcccc atctcttctt tgaccagctc acccagcgag
     4981 cgaccgtcac ggcgcgtaga aacaaacagc accatgaaat cctgcaccgc accggcgaga
     5041 accaccccgg cgagcagcca gatcatcccc ggcaggtagc ccatttgcgc tgccagcacc
     5101 ggccccacca gcggccctgc tccggcaatg gccgcaaaat ggtgaccgaa cagcactttc
     5161 ttgtccgtcg gcacatagtc cagcccgtcg ttatggcgca ccgctggcgt catacgcgtc
     5221 gggtcaaccg ccagcacatt tttggcgata tacagcccat aaaaacggta agcgatcaga
     5281 tagatacaga ccgacgccac cacaatccac agcgcgttga tctgttcccc acgatttaaa
     5341 gcaatgtatc ccagagcaaa tgctcccatt acagagagca ctgtccagac gaggtatttc
     5401 cctgatttgt tcatagttgt tatccgtgtg cgtgtccata gagatgttac attttgtttc
     5461 tataacatct ttgtacgatt taacaacacg gtaaccatgt aaatcggagt tgaaaccaat
     5521 atttaactta acaatgttaa ctcgatcact ccgttaaccg agttttcctg caatctcatt
     5581 taattctgtc gggctcatcc caaaactgcc gtacccagcc gacaagtgca gcaacgccgc
     5641 ccctgttcat cgaaaacgat gatttcccag ctctgatttt gccgcccaag atgcagcggc
     5701 tggcagacgc cgcgtacctt gccttcagac atcgggcgat gatgggtggc gttaagttcc
     5761 gtacccacca cacactgccc gtcacgggtc atcataaatc cggccatcga tcccagcgtt
     5821 tcagccagcg ccgccgacgc gccgccatgc agcaggccaa acggctgatg agtacgggta
     5881 tcaaccggca tttcggcttc cagcacatca tctcccagac gggtatacac aattcccaga
     5941 tgcgccacca ttgtgttatc gctggtggcg ttcagttcgt cgagcgttaa atggcgtttc
     6001 cagatcatgc ttatgccccc agcgttgagc cgccatcgac cacaatatcc tgtagggtga
     6061 tatggctggc gaggtcagag gcgaggaaca aaatcgtgtt ggcgatctct tgtggacggg
     6121 cgattttccc cagcggaatg ccgagtttaa actgctcgcc aaagccgcga atacgctgtt
     6181 cttcggcgtc atcgctcacc cacagcgtgc gttgcatatc ggtgtcggtg gagccaggcg
     6241 aaaccacatt acagcgcacg ccgctaaccg ccagttccag cccgacgctc aacgccaggc
     6301 ttttcagcgc cgctttcgat gcgccataag cactcatacc aatacgcggc gtgtgtgcgg
     6361 cgtcggacgc cacagtgaca atcgccccgc cccgctgacg gcgaaactgg ttcatggttt
     6421 gctgaaacag gttaaacgca ccgccgacgt taaccgcaaa agtctgctgc cagtcctctt
     6481 tactgagctg atcggtcgcg cccatgcgca aaattcccgc cgcattgacc agcacgtcca
     6541 gtcgctccgt ttcagctaac agtcgctgac atacctgcgc gacctgcgca gcatcggcaa
     6601 catccatcac ttcggtcgca aagggatatt gctcctgagc gaacgcttga tcaaaaccgg
     6661 taactttcgc tcccgcttca acaaacgcca gcgccgtggc gtagccgata cctttacctg
     6721 cgccggttac ccagacattt ttaccgctga aatccattat ttcacctcgc gggagagtag
     6781 cttccaccag gcgtcgatgg tcgggttttt cgccagcatg acaaagtcga tatcgccatg
     6841 cactttgcgc cagcgcgccg ccagcgccat catgcgcacc gaatccagac cgtagtcgat
     6901 caggttgtcg tcatcgaacg gttcatcgga ctcgtccagc aacggcagga taacctcacg
     6961 cagcgccgct ttcctggcgg ggacaggtgc tggcagtaat ccttcagtca tcaccacccg
     7021 gccagaccgc ccggccacat atttcagcga catcaaatgc tcgtcacggc tgaaatcggc
     7081 cagcgcatcc gccaccataa acggtttaat atcgcgcata aatgcgtcgg ttgcggtggt
     7141 catacagcca atgtgggcat ataccccggt aataatcagc tggttacgtc cactctcttt
     7201 gagcatttgc tccagcggag aacgatgaaa cgcgctgtag cgccacttca ccagcaccgt
     7261 gtcgtcggca tctggcgtca ggcgatccac caccttttgc tgttccggcg aacgggtcag
     7321 gcccggcccc cacatatcat tcaacagcgc ccgatcttca tcgctctgct ctttcggctg
     7381 ggcggtgtaa taaaccggga tattgtgctg tttgcagtag tcgcgcagcg cagcaatatt
     7441 cgcgatcacc tgctccatca tcgggcagtt ctcgccccag aagctgacaa aatagtcctg
     7501 catatcatgg attaacaacg cggcacgttg cggttcaaag gcccagtcaa ctttattctg
     7561 cggaatatcg tgagactccg gcagtgcgta agcctgtaat tttggaatag ccatcgtgtt
     7621 ctctccttca ggctgatgcg cgtgacgcca gccactgacg taattgtttt ttatcgactt
     7681 tcccgaccgc cgtcagcgga agtgaatcca cacactccac gcgatccggt aatttaaatt
     7741 cggcaatacc ctgttcacgc aggaaacgac gcacctgcac cgcgcgcagc ggctctttta
     7801 ccaccagata agcacagctt ttttcgccca tcagctcatc ttccatgctc accagtgcgg
     7861 catagatcac cgccggatgg cgcagcagca ggttttcgat ctcttcggca gcgatcttct
     7921 cgccgccacg gttgatctga tctttctcgc gcccctgcac ggtgatgtaa ccctctggat
     7981 caatagagat cagatcgccg gaacagtaaa aaccgttggc atcaaaggca ctggcattgt
     8041 gctgcggact tttgtaataa ccgcggaagg tgtacggccc gcgcgtcatc aggcgtccaa
     8101 cttccccttg cggcagtgga tttccttcag catcagcaac ccatacttca tcgtccggac
     8161 acattgggta accctgggta tggataattt tctccgcgct atcatcaagt cgggtgtagt
     8221 tcaccagccc ttccgccatg ccaaacacct gctgcaactg gcagccaatc tcagcggaaa
     8281 tacgcgccgc aagggtggca gaaagacgtg cgccgccgac ctgtaacagt ttcagcgagg
     8341 caagctgcgc ccggctttcg ccttcagcca gcgcctgcaa ccacaggcta actgccggcg
     8401 gcaccagcgc ggtaacgtta acctgatgtt ttgcaatcaa cgggaagcaa agcgtggcgc
     8461 tgggatcggc cgccagaaca accgttcctc cggcaagaaa gacgcccagc gatcctggcg
     8521 aactcatagc gtagttatga gccgccggga tcgcgcacag gtagcgtgtc tgttgtgtga
     8581 actgacaaat ctcgacgcta cgacgcacgc tgtagtagta gtcgttatga gtgcgcggga
     8641 tcagcttcgg tgtaccggtg gtgccgccgg aaagctggaa ataggccact tcatcagcag
     8701 gtgatggcgt agcagtaaaa ccgtctgccg gatggttaat cgcatcctgc aagttatgct
     8761 caccgctgtc gttgagcagt tgcaccacgc gaatggagga atgttctgtg acgaaagtat
     8821 tgaggaaatc atccccgcta aacagcgcat gttggcgatc ggcaatcagt aacgccggtt
     8881 caatctggct ggcataggcg ttcagttcac tacgctgatg gctgaacaac gccagcaccg
     8941 gcgcaacgcc cagtttcagc agtgcgaaaa aggtaatgta aagttcagcg acgttaccca
     9001 gttgtaccag cgcggtttca ccgggtttaa tgccctgacg gcgtaaacta cacgcgaggt
     9061 tatccgccgc ctgattcagc tcccggtaac tcaactgtcg ctcgccgtcg ataaccgcga
     9121 tgctgtcact cgcagcgtgg cgagtcagaa tgtcggtcag cggcaaatcc tgccagtagc
     9181 ctttttcccg atagcgacgg gcaaactctt ccggccagcg ggtgaatgga atgctcatcc
     9241 tcgctcctta atgcaatcca aaaacgttca acatggtaga aagtttgacg cctgtttcgc
     9301 gccactcacc caacggtgac gacgcaggca caatccccgc tccggcaaac agacgcacct
     9361 gattttcccg cagcttcgcg cagcggatgg tcaccaccca ttcgccgtta ccttcgctgt
     9421 cacaccaacc cacaatgccg ccaaacagtt cgcggtcaaa cggctccagt tcagcaataa
     9481 cctgggtcgc ggcctgatgc gggaaaccgc tcagcgcggg ggtcggatgc agcagacagg
     9541 ccagagtcag tgcgttttct tgcgaattcg ctttaccttc aaagggagtt gcgagatgcc
     9601 acagcgtcgg cgtggtaatc aattgtggag aggagggaac gtgtaactca ctactgcgtt
     9661 cgcgcagtac ctctttcatc gcctgagtca ccagttcatg ttcatggcga tctttttctg
     9721 acgccagcag acgattaccc gcttcgcgat cgagcacttc atccggctga cgacgcgcgg
     9781 aaccggctaa cggaatggag ctaaaacgct cgccgtcttt acgtagcagc agttccgggc
     9841 tggcccccag caggacgcca ccatcagcca gcgggacatg gaagttgtaa ctaaccgggt
     9901 tttgcgcaat caaccgttcc agcaatacgc cactatcaat ggcggcgtca gtggtgatat
     9961 caatcaatcg tgacaacacc actttgtcga cctgcggcgt ggcggtaagt gcggcggcgc
    10021 gggcaaccat ctgttcaaac gtggtttgtt caggaattgc ctggcgttcc accacgttca
    10081 gcgactggct gcgggtaaaa cagtgggctg aggtctgttt ttcctgacgg gagaacgact
    10141 gccaggattc gggaatatac agcgacgaag gctgacgtgg atcgaaggga atcgccccga
    10201 ccatcaccgg atttttgatg ccctgcgctt tggcatcggc aaacagcgcg gcgagttttt
    10261 gctggaaggg actgtcgggc gaatccccgt tcacagccgg ttcatcgaag cgggcgaaac
    10321 atcctgacgt cgtaaaactg cggtacggcg acataaagaa aaagcgattg ggcgcaagtg
    10381 ttgccatggt ctgctgtact tcctcagcca gtgacgtatc catatcatcc tccacaaaat
    10441 gataaaggct ttaataatga ttatcattta tattttcggt cgctaaccta aaagcaaacg
    10501 cgcactatgt caactcttga ggtaacgcaa tctgcaactc ggggttgcat ctggttgtgg
    10561 ttacaatgaa aatgagaagc attattgatg aattcgcata agcgcaatgt gatgtcctgc
    10621 gccgttttgc cccctctccc ttccagggtg agggctgggg tgagggttaa tgttcgcacc
    10681 agtgctggct gttcccctca ccctaaccct ctccccaaag gggcgagggg acggattgtg
    10741 cgctttgtcg aatttgtcat tacgccctta accttattaa taacaggaag ctgacttgtg
    10801 agactcgccc cgctctaccg caacgccctt ctattaacag gacttttgct ttcaggaata
    10861 gccgtagttc aggccgccga ctggccgcgt cagattactg acagccgggg cactcatacc
    10921 ctggaaagcc agccgcagcg tattgtttcc accagcgtca ccctgaccgg atcactgctg
    10981 gcgattgatg ctccggtgat cgccagcggc gcgaccacgc cgaataaccg cgtcgcagat
    11041 gaccagggct ttttacgcca gtggagcaag gtggcgaaag aacgcaaact gcaacggctc
    11101 tatatcggcg aaccgagcgc cgaagccgtt gccgcgcaaa tgccggatct gattttaatt
    11161 agcgcaaccg gcggggattc ggcgctggcg ctgtatgatc agctttccac catcgccccg
    11221 acattaatca tcaattacga cgacaaaagc tggcagtcgc tgttaacgca gcttggcgaa
    11281 attaccgggc atgagaaaca agcggcagag cggattgcac agtttgataa gcaactggca
    11341 gcggcgaaag agcaaatcaa attaccgccg cagccggtca ctgccattgt ctataccgcc
    11401 gctgcacaca gtgccaatct ctggacgccg gaatcagcac aagggcagat gctggaacaa
    11461 ctcggcttta cgctggcgaa gttgcccgca gggttaaacg ccagccaaag ccagggcaaa
    11521 cgccatgaca tcattcagct tggtggggaa aatctggctg ctgggttaaa cggtgaatcg
    11581 ctattcctgt tcgccggtga tcagaaagac gccgatgcga tttatgccaa tccactgctc
    11641 gcgcacctgc ctgcagtaca aaacaagcag gtttatacgc tgggaaccga gacgttccgc
    11701 ctggattact acagcgccat gcaagtgctg gacagactta attcactgtt aaaaaaagat
    11761 tgataaaaca tacaattaga gcactaataa tcccctcgcc cctttgggga gagggttagg
    11821 gtgaggggaa aatgcacctt tagctaactg tcagacgctg tcacctgcgg cggcgtctgg
    11881 cgaaaacgtc gcaactccac cagcaccagc aataacaaca cgccgataat caacaaacca
    11941 aaaccgctcg cgcttgcgga agcaaccggt gtcatcatcg cgcccaaacc acccaacagc
    12001 gccgcgccta tggcatcgcc cgtcacgttc tgcgccgtcc acaaaccgtt aatccgccct
    12061 aacatcgctt ccggggtttg cgtttgcagc attgtatatt gcagcaacga actaaccgcg
    12121 ctcagccagc cgaacagcgc cagacaaacc acgcctaaaa tccacatcgg catcaggcca
    12181 aacagaccaa tggcgaggaa cgatcccagc gtggagagca gcatcaataa ccctggtcgc
    12241 gcactatgtg ccagcttccc gctggttaac gcaccaatag ccgcaccgag cgggatcgcc
    12301 gcatagagaa aaccaatctg tgccgccgac atctgccagt tgtcagccag cgccggatac
    12361 agtacccgca ccgcgctcgc catcgtcaat aaaccaccca gcaacgcaat ccctcctacc
    12421 agcgggctgg cgagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcgcgc
    12481 ggctgcggtg gcggtggcaa cgccggaagg cttaacaacg gtagcaaggt aataaacgtg
    12541 cccgccgccg ccagcccgta gttccaggct acgccgccgg tcgccagcaa taaaccgcca
    12601 atcatgggcg aaatcaccga ccccagacgc acggtcaaca tggtgatcgc cccggcctgc
    12661 attaagtttt cacgccctac cagtgcaggt gttgccgcca gtagcgccgt aacaccaagt
    12721 gatgcgaaaa aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc
    12781 agcagcgcgt tcaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa
    12841 atcacttttt tgcgctcata gcgatccgcc agcacaccgc caaccatcag gccaacaaac
    12901 atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc aggtagaatg cgtcatcatc
    12961 tggatctgca ccggcaccgc gacgccgagc aaacccagag acacaattga gataaaacga
    13021 gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag
    13081 gattgtttat tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg
    13141 cacatgctaa catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt
    13201 tcgtatgtct ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt
    13261 actgattatc gcgacggcat taagcctgct cattggggca aaatcactcc ccgcgtcagt
    13321 agtgctggag gccttctcag gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc
    13381 gcgactgccg cgcacccttg ccggtttact tgcaggcggt gcgcttgggc ttgctggggc
    13441 gttaatgcaa accctcaccc gaaatccact tgccgacccc ggcttgcttg gcgtgaacgc
    13501 cggagccagc tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcgcagga
    13561 acaactggcg atggccttcg ccggggcgct ggtggcctca ttgattgttg cctttatcgg
    13621 cagtcagggc ggcgggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc
    13681 ggcggtgctg gaagggctga ccagcggcat cgccctgctt aatcctgacg tctacgatca
    13741 actgcgtttc tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt
    13801 gctgatcccg gttctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag
    13861 tttgagcctc ggcagtgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt
    13921 gattggtctg ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat
    13981 tgcctttatc ggcctgatga tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg
    14041 ctggtcgctg cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat
    14101 cgggcgggtg attgtgcccg gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc
    14161 accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct
    14221 cgccgattac tcatcacctg tttgctgctg atttccgcct gtgtggttgc aggtatctgg
    14281 ggattacgca gcggtgccgt cacgctggaa atctcgcagg tattcgccgc gttgatgggc
    14341 gatgcgccgc gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg
    14401 gcgctgttga ttggcgcggc gctgggcgtc agcggcgcga tttttcagtc gctgatgcgt
    14461 aacccgctcg gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg
    14521 gtggcgatgg tgctgtttgg tcaggacctg acggctatcg cgctggcagc aatggtgggc
    14581 ggcattgtca cttctctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt
    14641 cggttgatta ttatcggcat cggcgttcgc gccatgctgg tggcctttaa tacttggctg
    14701 ttgctgaaag cgtctttaga aacggcgcta acagcgggtt tgtggaatgc cggatcgctc
    14761 aatggcctga cgtgggcaaa aacttcgcct tccgcaccca tcatcatatt gatgctcatt
    14821 gccgccgcct tgctggtgcg gcgaatgcgc ttgctggaga tgggcgatga taccgcctgc
    14881 gcgctgggcg tcagcgtcga gcgttcgcgt ctgttaatga tgctggttgc agtggtgctt
    14941 accgctgcgg caacggcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac
    15001 attgcccgac gcattagcgg caccgctcgc tgggggctga cccaggcggc gctgtgcgga
    15061 gcgctgttac tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt
    15121 ccggttggcg tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag
    15181 gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg
    15241 gatatggcaa atataccgtt gcggaaaatc tgactgtaga aattcctgat ggtcacttca
    15301 cggcaattat cggaccaaat ggctgtggta aatccacgtt actgcgtacc ttaagccgcc
    15361 tgatgacgcc tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca
    15421 gtaaagaagt tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata
    15481 tcaccgtgca ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct
    15541 ggcgcaaaga ggatgaagaa gcggtaacga aagcgatgca ggccacgaga ataactcatc
    15601 tggcagatca aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatcgcga
    15661 tggtgctagc ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata
    15721 tcagtcatca gattgatcta ctggaactgt taagcgaact gaaccgcgag aaaggctata
    15781 ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg
    15841 cattgcggga agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac
    15901 tgattgagcg catttatggt ctgcgatgca tgatcattga cgatccggta gccggaacgc
    15961 cgcttgtggt gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt
    16021 ctgccatcat ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac
    16081 aagccaccat cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt
    16141 cttttttcac tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt
    16201 tgacatttgc ttttgttaat tgctcgacaa gatactgccg attacgtaat tcaccgttca
    16261 gttccgcaac gtcagtaacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc
    16321 cgagagaaat agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct
    16381 ttttaattcc tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat
    16441 caagttgatt tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt
    16501 tgatctccag tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat
    16561 aatcgatata cccgctcaaa acggtctgcg cctctttact ggttggcgcg gtaaaactta
    16621 gcgtccagga ggtatacagt gacggttcat cttttttctt actggcattg tcatcaaccg
    16681 ctttcatttt ttcgctcaat gcaacaatag cgcgatgcaa atccagttca tcgattttcg
    16741 cctcttttaa ttggtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa
    16801 cagactgaaa cttcttgata aacaggttaa atgcaccgcc acggtcgata ttaacatcga
    16861 gatcaagaac gcggagtttg gtaaaggttt tctccaggtc ctgccactga atggcttcgg
    16921 cgggagtaat aacggctgag ctggtccatt tttgcggcag gatgaaagag atcagtaaac
    16981 ctgcgcaggc aaatgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt
    17041 cgattagatt aagtaaatca atttcattat tactgggcga cgccagagga tactcagaaa
    17101 aatgagcgtc acttccctgt ttaatattca atgatgacat ggggtataac ctgaaaatga
    17161 gtccaatagg ggaaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag
    17221 ctgaagtttg taaattcctt ccactgaatg caatattcac tctatttaag agaaatattc
    17281 atcaaaccta taaatcaaac aaataaaaaa cccataatta ttttctgtaa ttatgggttt
    17341 atagataata ttaatttacc tgtttagcgt tgcgcgaata atcggcccaa tttttacaaa
    17401 cgcccctgga gagataatat ccacatgcgc acaatcctga cgatagatat ccagctccgc
    17461 aatccacggc gaccaggcgc gttcaggact cataccttcc tgaagcgtgc gttcagcaac
    17521 aaacaacgtc gctttaccgt caaacggtac gctatgagca gtcgtcagca ggcgcacagc
    17581 atcagcgtag ttgccttcaa tggtggtaaa caactccgtt gaagtacttc cctgctgtgc
    17641 cgccaggaag gcctcgcgct cgcggttaat ctccgccagc acttccgggt ccagaccatt
    17701 agcttctttt tcctgccagt tttgcgtttc tggcggccag gtatccagca agccaagaaa
    17761 tgccacctgt tcgccacggg cacgcagtcg cgccgcaata ccctgcgcca gcgtaccgcc
    17821 aagggaatac cccagcaggt aataagggcc gtgcggttgt tgctcaagta acgttgccag
    17881 atgcgcttcg cagacttcat ccaggtttgt cgctgtctgc atgggtccat gagggcgcgg
    17941 cgactgaatg ccaataatcg accattgtgg atcgagataa cgcgagagta cgctaaactg
    18001 ccaggcaaaa ccggacgccg gatggaaaca aaacagcgtc gggccattac cttcacgcaa
    18061 cggcagaatg gtttcgaatc ccatgcgccg ggagctgtcc tcttcaccat caataatcgt
    18121 tgccagtttg gcgacggttg acgcgaccat cacctgcccc ggcgtcacct gacgagcaaa
    18181 ctgtcgactt aactgcgctg ccagtttcat tgccagtagc gaatgaccgc ccagcgcaaa
    18241 gaaatcagca tcggcatcct gcacgtcaca acccagcaac gacgcgaatg ccgcggcgat
    18301 aatcgtttca ctgcccgctt tcggtgcacg tcccgacgct tgtgtcttca gttcaggcaa
    18361 cggtaaggct ttgcgatcca gcttgccgtt ggcgctaaga ggtaactgtg gaagttgcag
    18421 cagaaccacc ggtaccatat gtggtggcaa tgtttcacga agctgcgcct gcaatgcgct
    18481 ggtatccaac ggcaggcccg attgcgacac cagatagccc accaactgac gcgcatcacc
    18541 accagtggct gccgcctggt taatcacaca ggcgtgggta acggcttgtt cgacatccgg
    18601 cagcgcctgc atcacgcgat cgatttcgcc cagttcgata cgctgcccgc gaatttttag
    18661 ctgatcgtca ctgcgcccga ggtactccac cgcgccgtta tccagccagc gggcaacgtc
    18721 tccggtacgg tacatccgtt cgccaggagc aaaaggatcg gcaataaagc ggctggcggt
    18781 cagatcgggg cgtccgagat agccctgcgc cagttgaatg ccggtaagat agagatcacc
    18841 cgccacaccc ggcggcaccg gatgcatcat cgcatcaaga atacgcagcc ccatgttcca
    18901 caccggataa ccaatcggca cactgctgcc gcgcacctgt gccagttcct cgccaaaagc
    18961 cggataccag ctgacatcta ccgccgcttc cgtcgggcca tacagattat gtaacggcgc
    19021 accggttaac tgttgccatt cacggcataa atcagccggt aaggcttcac cactacagaa
    19081 aacctgtttc aacgtcgcgc aactctggcg agcggtttgc ggagtcagcg aggcaacaaa
    19141 tgccgccagc atcgacggca caaagtgcgt ggtcgttacg ccatattcgg caaagaattg
    19201 ctgcatagcg agcgggtcac ggtgcgcttc cggttcagcc atcaccagtt ttgcccctgc
    19261 gataaacggc cagaaaaact cccacaccga gacatcaaaa ctgcacggcg ttttttgggc
    19321 aacgacatct tcgcctgtaa gcgggtagtg attttgcatc cataacaggc ggttaacgat
    19381 agccgtctgc ccgaccatta cccctttcgg cctgccggtg gagccagagg taaagatgat
    19441 ataagccgtg tggtgcggtt gtgaaagttg cagcggcgca ctgccctggg gggtaagcgg
    19501 ggcgttatag caaaggcttg ttaaattggg aacatcgcta aagcgcggca gttgatcgtc
    19561 ggtggtaatt aacagcgacg gacgcgcatc ttccagcatc attttcaggc gatcgtccgg
    19621 atagccggta tccagcggta gccaggccgc gccagcttca actattgcat ggagtgccag
    19681 ggtcaaaaag accgagcgcg gtagtgccac cgccacgctg tcccctggtt taacgccgcg
    19741 ctcacgcagc agattcgcca gcgccaccac ctgctcgcgc atttcccgat agctgaacag
    19801 gtaacgcgca tctgccagcg ccggagcatc cggtgttttt gccgcttgtt ctgccaccag
    19861 cgcgctcagc gtggtttctg gaatctcaac ctgagtggcg ttgatctgcg ccagctgcgc
    19921 atactcacct ggcagcataa tatcgacatc gccgcacaac agcgccggat ccgcggcgaa
    19981 ctgggcaatc agcattttca ggcgttcagc atgctggatt aacgttggct catcgtaacg
    20041 ctgtttattg gcgaggatct caatactcaa atcaccgtgt tcatccggga acagggccag
    20101 ttcaaggtca ttaaccggac cggttgccag ggtatgggtt tgcgcctgaa caccaggaat
    20161 atccagttgg taatcaaata ccttaatatt gagtaccgga ccaaacagcg gttcatcacc
    20221 tgccgctcgc ccgctgtcac ggacaatttg ttcggcatcg taacgttgat ggcgacgcat
    20281 ttttttcagt tgagccgcca gtcgggttgc cagttccggc agcgtttctt gcgccgcaat
    20341 gtgaataccc aacggtaaaa cgttgagcac gggtccgcta gccgtcagcg ccgccgagcc
    20401 cagtcgacgc ataaagataa atccggcggc atagtccatg cgattgcaca atcgccccag
    20461 ccacaaggct gccagcgcaa gggctaaatc ggtacgctgc acacctgaaa gttgcgtagc
    20521 cagctggcgg aattccccgt cggtaaattc cagtttcagg cgcagaatat ctgccgaagc
    20581 gctgcgcccc ggtaaaggtg ccggagaaag tgacgcgggc ggcggcagtt gacgacgctg
    20641 ttctgcccag aatgccgcat cacgctgcca ggcttcgctt tcgcggtatt gctggtactc
    20701 ttccactaca tcagcgaaag gcgtaaacgg cgaagcaggc gttggttcgc cacgcagcca
    20761 tgtgcagtaa atattggcga tctggcgagt aatggccggg aaactgaagc catcgaccag
    20821 caaatggtga taacgctgat accagtacca gcggttatcc gcaacatgta tcagctgatg
    20881 aaagaccagt ggtttaccgc tatcgacgcg caaatcttgt tgcaaatccg ccagcattaa
    20941 tgcctgcgca gtaccgtgcg gatcaatatt ggttcgcagg tcgataattt ctggctgttc
    21001 gaatatcagc gcatcatcga cccactgcca gacttcgccg ttatcttccg taaaacgcat
    21061 ccgcaacgta tctgcttgcg ctagtccggc aaccaccgcg cgggccagta atggcgcatc
    21121 aacatctcca gttaactcaa cgtaatgcgc cacgctccag gcggagggta aatccgacag
    21181 tttttctgcc atccagatgc cgggctgtgc ggcgaccaaa ggtaaatgct ggctcattgt
    21241 gcctcctgca actgggtgaa attggccggt gtcagagtac gccagtggac ttccagccac
    21301 tgctggcagg acgcctgtga ctgcggctga cacacaatgt cccagcctgc cgataagagg
    21361 cattgttgcg gccacagact gaattgcccc tgcgcattgc gcaaaatgta aaacgctccc
    21421 tgcggatcat cgaagggatt actgaatgcc atactcaact cctgtcatgg aaaagtggtt
    21481 gccagaggtc gattaacccc tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc
    21541 cgtcaacctg acgccagaaa atggattctt ttatagggtg caattgcgca tacagcgcct
    21601 gattggcccg catgatcatc ggctcgcgaa tacccgcttc cagcacaatg cgcagacctt
    21661 cggcgctaac ttcaccagct tttagctttt caagtaacac gccctcttgc tgcccgtccc
    21721 gatgcggcca ccagtacgat cctgactggc ttaatacaca gccaaagcgt tcaggccagt
    21781 gcagtccggc atacagcgcg gacagcccac caaaactctg cccggcgacc acggtgcgat
    21841 cggcgcgatc gctaaaaggg gcaatagctt tcaccagggg taataactct tgctgtactg
    21901 ccagccagaa atccgcatta cacggcagtt cgtgggcgcg gtgcgtggtg tcgatagcgt
    21961 cgatcaacac atacacggcg ggaggaagtt gctgacgatg ggtcagcgaa gtcagcacag
    22021 gccagacggg catactttgc gcccaaaatt cgccatcgag caaaactgcc agcggacgtt
    22081 cttctgctgt tacatcgccg gtggtaaaaa tccatacacg acgtgaattt ttcaaccgtt
    22141 cacttttcca gataatttct ttggctgcta tttctggcac ttgcggacaa tcccatcccg
    22201 gttgcagagg cgcttgcggc atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc
    22261 agctttgtgg attcagcgga tcggctattg cctggggtaa tagttttcgc cagccttcac
    22321 gtaattcgag gcgatcgggg gatggtgcag aaaaaatgtc atcgcgttcg gtgggaataa
    22381 agcagtagct gccgcgccag ttggcattga gttgtgtcgt ccactgccag acatcagtac
    22441 ctgcaattcg ctgcatcgac tggggctggc tgttctgatg atgatcggtc acaccagtga
    22501 tgtagatcca tacgcgcttt atcgtcgagt attcttcaga accttgggga tcacgccacc
    22561 agaaagtgac ctcaaacatt tcgtcattca gacgctgcca ttccgggcca tgtttcgact
    22621 gccaccagct ctcacttcct acttttaacg ccgtcacacc ataaccccat gtttactgtg
    22681 caatttttca ttgattgcag aaatatattg ataatattat tgataaccat ttgcatttgc
    22741 aatagcgtaa tggcgcgccg tgggaagcgc ggacatttat taaccaactg cactgcgtgt
    22801 ctttcaggat caaaggtttt cgcggtagcg ggatgcgtcg tgttgaggac gaccatgccc
    22861 gacagttgca attcgtggca aaaatgcagg aataaaacaa tgaacaagaa gattcattcc
    22921 ctggccttgt tggtcaatct ggggatttat ggggtagcgc aggcacaaga gccgaccgat
    22981 actcctgttt cacatgacga taccattgtc gttaccgccg ccgagcagaa cttacaggcg
    23041 cctggcgttt cgaccatcac cgcagatgaa atccgcaaaa accccgttgc ccgcgatgtg
    23101 tcggagatca tccgtaccat gccaggcgtt aacctgaccg gtaactccac cagtggtcag
    23161 cgtgggaata accgacagat tgatattcgc ggtatgggtc cggaaaacac gctgattttg
    23221 attgacggca agccggtaag cagccgtaac tcggtgcgtc agggctggcg tggcgagcgc
    23281 gatacccgtg gtgatacctc ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg
    23341 cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt
    23401 accaaaaaag gcagcggtga gtggcacggc tcctgggacg cttatttcaa tgcaccagaa
    23461 cataaagagg aaggtgccac taaacgcacc aacttcagcc tgaccggtcc gctgggcgac
    23521 gaattcagct tccgcttgta tggcaacctc gacaaaaccc aggctgacgc gtgggatatc
    23581 aaccagggcc atcagtccgc gcgtgccgga acgtatgcca cgacgttacc agccgggcgc
    23641 gaaggggtga tcaacaaaga tattaatggc gtggtgcgct gggatttcgc gccattgcaa
    23701 tcgctggaac tggaagcagg ttacagccgc cagggcaacc tgtatgcggg cgacacccag
    23761 aacaccaact ccgattccta tacccgctcg aaatatggcg atgaaaccaa ccgtctgtat
    23821 cgccagaact actcgctgac ctggaacggc ggctgggata acggagtgac caccagcaac
    23881 tgggtgcagt atgaacacac ccgtaactcg cgtattccgg aaggtctggc aggcggtacc
    23941 gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg atatcgatct tgatgacgtg
    24001 atgctgcaca gcgaagttaa cctgccgatt gatttcctcg ttaaccaaac gctgacgctg
    24061 ggtacggagt ggaatcagca acggatgaag gacttgagtt ccaataccca ggcgctgacc
    24121 ggggcgaaca ccggcggcgc tattgatggt gtgagtgcca ccgaccgtag cccgtattca
    24181 aaagcagaaa ttttctcgct gtttgccgaa aacaacatgg agctgactga cagcaccatc
    24241 gtaacgccgg ggctgcgttt cgatcatcac agtattgtcg gcaataactg gagcccggcg
    24301 ctgaacatat cgcaaggttt aggcgatgac ttcacgctca aaatgggcat tgcccgtgct
    24361 tataaagcac cgagcctgta ccagactaac ccgaactaca ttctctacag taaaggtcag
    24421 ggctgctatg ccagcgcggg cggctgctat cagcaaggta acgatgacct gaaagcagaa
    24481 accagcatca acaaagagat tggtctggag ttcaaacgcg acggctggct ggcgggcgtc
    24541 acctggttcc gtaacgatta tcgcaataag attgaagcgg gctatgtggc tgtagggcaa
    24601 aacgcagtcg gcaccgatct ctatcagtgg gataacgtgc cgaaagcagt ggttgaaggt
    24661 ctggaaggat cgttaaacgt accagttagc gaaatggtga tgtggaccaa taacatcact
    24721 tatatgctga agagtgaaaa caaaaccacg ggcgaccgtt tgtcgatcat cccggagtac
    24781 acgttgaact caacgctgag ctggcaggcg cgggaagatt tgtcgatgca aacgaccttc
    24841 acctggtacg gtaagcagca gccgaaaaaa tacaactaca aaggtcagcc agcggttggc
    24901 ccggaaacca aagaaatcag tccttacagc attgtgggtc tgagcgcgac ctgggatgtg
    24961 acgaagaatg tcagtctgac cggcggcgtg gacaacctgt tcgacaaacg tttgtggcgt
    25021 gcgggtaatg cccagaccac gggcgatctg gcaggggcca actatatcgc gggtgccggg
    25081 gcgtatacct ataacgagcc gggacgtacg tggtacatga gcgtaaacac tcacttctga
    25141 tgctaacgtc agattgttga caaagtgcgc gtcgttcatg ccggatgcgg cgtgaacgcc
    25201 ttatccggcc tacaaaaatc ttgccaattc aatatattgc aggaccatgt aggcctgata
    25261 agcgtagcgc atcaggcagt tttgcgtttg tcatcagtct cgaatatggt cgatatgaaa
    25321 actacgcata cctccctccc ctttgccgga catacgctgc attttgttga gttcgatccg
    25381 gcgaattttt gtgagcagga tttactctgg ctgccgcact acgcacaatt gcaacacgct
    25441 ggacgtaaac gtaaaacaga gcatttagcc ggacggatcg ctgctgttta tgccttacgg
    25501 gaatatggct ataaatgtgt gcccgcaatc ggcgagctac gccaacctgt ctggcctgcg
    25561 gaggtatacg gcagtattag ccactgtggg actacggcat tagccgtggt atctcgtcaa
    25621 ccgattggca ttgatataga agaaattttt tctgtacaaa ccgcaagaga attgacagac
    25681 aatattatta caccagcaga acacgagcga ctcgcggact gtggtttagc cttttctctg
    25741 gcgctgacac tggcattttc cgccaaagag agcgcattta aggcatcgga gagacaaact
    25801 gaagcaggtt ttctggatta tcagataatt agctggaata aacagcaggt catcattcat
    25861 cgtgagaatg agatgtttgc tgtgcactgg cagataaaag aaaaaatagt cataactctg
    25921 tgccaacacg attaattgac aacatctggt acgattcgcc cgcagccatc actgactgcg
    25981 ggcgaaagtg taaagcaggt gccttaccac tctgacctga caaccggata tgcagggatt
    26041 gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
    26101 tacgttcctt tacggtgaac tcacacagcg agtctccgac cagaagcgta aatcccagca
    26161 ccgtcaaaca cagcactatg actgccacaa gggcatattt cgtcagcatg tttatattgc
    26221 ctccatgcga agaagggact accatccgag ttgttgaggt tcggagtggc agccccagga
    26281 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
    26341 aaagcttcag gcacccgccg ttatcttacc cttcctcagc aaaatatctg tttatttata
    26401 cagtaagtcc tgtttcggac ttctgttggc aacactcgtt attgctcgta aaacgaaatc
    26461 gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccctac catcctgacc
    26521 tgacaaccgg atatgcgggg attactcccc gcacaacggc tacttcttcg gttcgtaagc
    26581 gagaacagcc ttaaactcaa tattacgttc cttcaccgta aactcacaca gcgagtctcc
    26641 gaccagaagc gtaaatccca gcaccgtcaa acacagcact atgactgccg caagggcata
    26701 tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc aaattgttcg
    26761 ggtttggagt ggcagcccca gggttgatag aaatatctcc tggggcttca tctttccaga
    26821 cctcagagta aacctgaaac cggaaagctt caggcacccg ccgttatctt acccttcctc
    26881 aacaaaatgg ctgtttattt atacagtaaa tcctgtttcg gacttctcac tcctcattta
    26941 atcaactgaa gctgagcccg ttttttctgc atgcctcaat cgtcaaaatt cttcgccatc
    27001 agccagctct gccataactt gccacattga aaaagtagcc aaattgcttc agtcaccatc
    27061 atcaaaaaaa gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac
    27121 tgaatagaaa acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga
    27181 accttttacc ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt
    27241 aagccaggac tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt
    27301 aaagcacgat atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa
    27361 ccaggctgcc gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag ccgcagacca
    27421 tcatctggaa atttgcggcg gtggcacaca cccgtttcag aaatggcagc gtcaggaggt
    27481 atgcgataac gagcgctatc aacgaacgct ggaaaacttt ggctatctca tccagcaggc
    27541 gaccgttttt ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt
    27601 gctgcacggc ttgtcacggt ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata
    27661 tatgcaagga acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc
    27721 cgataatggc ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg
    27781 tttgagttac accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag
    27841 tcctcatttt ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc
    27901 ggtaaatatg gcgggattaa ttcaggctac cgcccactgg ttactgacag aacgcccgtt
    27961 caaacatcag gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta
    28021 tgggctggaa ggcgtcatta ccgatccgca cactggcgat cgtcgaccgc taacggaaga
    28081 caccttgcga ttgctggaaa aaatcgcccc ttccgcacat aaaattggtg catcgagcgc
    28141 gattgaggcc ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga
    28201 tttcgtcgcc gatggtggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc
    28261 cgatgactaa gtagggaatt gccttacaga agaatatccc tgacaatggt gttttaactt
    28321 acatttaaca atgttatgaa acacccttta gaaaccttga ctaccgcagc aggcattttg
    28381 ctgatggctt tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca
    28441 caaaaactgg tgaccatgtt ccatctgatg gatcttagct agctttacac tttattgttt
    28501 tgtctgtggt ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga
    28561 cgctggttct cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc
    28621 ttattctcct tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa
    28681 acgcctggcc cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga
    28741 tgtttttaaa attggcggca aaatttttat gctgtcgtcg gagctgcgcg gcgtcccctt
    28801 tatcaatctg aagtccgatc cacaaaaatc cctgttaaat cagcagatat acccgagcat
    28861 taagccaggg tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat
    28921 ctccgaagcg ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct
    28981 ggctaaacgc gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt
    29041 tctcgcttac catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca
    29101 tggagtcttt atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga
    29161 tgccagtaaa aaacttaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag
    29221 cccatccagc accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa
    29281 agcgcgtgtt gccaaatccg ctgccggtaa ttatgtgttc aacgaacgta aaatacttga
    29341 tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct
    29401 ggttgttgac caggaagatg ctgatggccg ctttgccacg ccggaagcga aagccgcgaa
    29461 cgataaaggt cgcaagttct tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga
    29521 gtggatggca aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggctct
    29581 gggtctggac gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg
    29641 tctgaaagag aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga
    29701 agattttaac gctacgctgc cgaaatctcg tctgccgcaa aacatcacct taaccgaagt
    29761 gtaattctct cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca
    29821 taaccctgtt tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat
    29881 ttaatatccc aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg
    29941 atgattttcg gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg
    30001 gtactgcgga ccttcgaaaa acgagccatc gccagttcac ggctttggtt gcaaatcata
    30061 acccagaata aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt
    30121 caggcggtat tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg
    30181 cagttgtgga tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg
    30241 aatctggcgc agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg
    30301 attaaactga tcggcgcgat cctggtcggc attttgatga tctcgctgct gattggtcag
    30361 tcgccagcga ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt
    30421 aaagatccga ttcttggtct ggtggccggt attcagcttt ccgcgaacga tatgctgaaa
    30481 ctgggcgact ggctggagat gccgaaatac ggcgcagatg gcgcggtgat cgatattggg
    30541 ttaaccaccg tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct
    30601 ctggtttctg actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt
    30661 aagcgcagta tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa
    30721 cgtctgaata aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat
    30781 gagtggaatc gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat
    30841 attggaacct ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa
    30901 gatatgacct taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc
    30961 tacgcgttta ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc
    31021 gatcacatat ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc
    31081 aatgatattc gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg
    31141 aatggatgag agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca
    31201 gcgttttaaa tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc
    31261 gcatatcatc catcgtgcac atcagcagca aaatcatgcc gaggaaggca atgcagagat
    31321 agtttccgaa cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg
    31381 ctgcacgaaa acgcagatgc gccagacaga tcataatcca gttcaacagc agcgttgcta
    31441 ccaccagcgc catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca
    31501 ccagcgaagt gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga
    31561 cgcgagtcaa aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt
    31621 tagagtaaac cccgctgtta tacactgaca gcgatgctac cagaatgacg aagttcagcg
    31681 cagaagctac cacgttgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg
    31741 atttcacttc cacccacgga tagagcgcca gtaaaaccac cagtgaaccg atgtaaaaca
    31801 gcaggatgcg atacaccacc tgatttaccg cttttggaat gcttttatcc ggatcgcgcg
    31861 cttcagcggc agtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca
    31921 gcgacaaaat cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt
    31981 cgatactggc tttctcgccg ccgtgaccag aaaacagcag ccacaggcca aagccgatca
    32041 taccgatgat tgccagcact ttaatcaacg caaaccagaa ctcggtttcg ccatataagc
    32101 gcacgttcac caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg
    32161 ttggaacatc cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc
    32221 ccaccagcac gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc
    32281 agtatttata ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc accatctcgc
    32341 caagctggcg cataatcagg aaagcgatga tcccggcgac gccgtaaccc agcaatacag
    32401 ccggacccgc catctgaatc gccggaccaa tgccaagaaa cagaccagta ccaattgcgc
    32461 cacccagcgc aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt
    32521 gattcgacgc agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt
    32581 cttttttgtt gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac
    32641 acggttaaac ggggtatcct gcttttattt ccgtacccga tgtcggtgca gccacatcag
    32701 cttatacgcc gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat
    32761 catcggtgcg gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg
    32821 cagcagaccg gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcagtaccgc
    32881 gccgtgatac aacgcctcat ccagcttctg ctcactgaat gtttgcggat tattcaacga
    32941 cggctcggcc tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc
    33001 gaccccggca agggcaataa aaccggtgcc cgttgccaca gaaagatgaa agcccatcca
    33061 ccacagcaac cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc
    33121 ttcgcccacc cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg
    33181 caccatgagt ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc
    33241 cacgctggtg cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac
    33301 caccgacacc atgtcacgat cgcgggcatc aatataaatc cagctcgtcg ggcgcgcatt
    33361 ctcggttttc agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt
    33421 gatttgctgc ttcatcggcg tcaggatcgg tagctggcgc agtgcctgcg ggctatcgcg
    33481 ccagctttgc ggataacgca gattaatggg ataacgggca atcccttcca ccgtttcgcc
    33541 aaccatcgcc ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc
    33601 gtaacgcgcg gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagacg
    33661 ctcggctaga gcagaagcca cgcttggcac cgttcgcgct acttcttcaa tttgctcagc
    33721 catcgcgtcg atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat
    33781 gccggttgag agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc
    33841 cggcagccgc acggtgttat ccagttcctc aatgattttg tccatcgtca tgccaggccg
    33901 ccactgatcc tgcggcttaa gctggatggt cgtttcaacc atctcgagcg gagcagaatc
    33961 ggtggcggtt tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat
    34021 aattagcttg tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag
    34081 cgtcgatggc atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac
    34141 tttattgagc ggccagagaa ccgtcagcac cgaaagcgct gccaccaaca gcgtggtttt
    34201 cggccagtgc agtactttca gcaatagagg atgataaaca cgaatcaaaa agcgattgag
    34261 cgggttactg ctttccggcg gaattttgcc acggatccag taccccatca ggatagggat
    34321 cactacgatc gccagcagcg ccgcgcccgc catcgcatag gttttagtga acgccaacgg
    34381 gccaaacaga cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat
    34441 aatcagcaga ctgataaata gcgccggacc cacttcaaca gatgcatcgg tgatcacctg
    34501 ccagcgcgtt ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg
    34561 tttatgtgca ttttcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc
    34621 aatgccgccc aacgacataa tattggcatt cagtccctgg aagtgcatga caataaaagc
    34681 aatacacaac cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag
    34741 aaacagcgca cagactaccg ccaccacgat aaactcttcc agcaacttgc cgctgaggtt
    34801 gtcgatggcg cggtcaatga gctggctgcg atcgtatgtt gtaactatct ccacgccttc
    34861 cggcagacta cttttcagcg tttccagttt gtccttcacg gaggcgatca cttctcgggc
    34921 gtttttgccg gagcgcagga tcaccacccc gcccgccact tcgccttcgc cattcagttc
    34981 ggcaatgccc cggcgcatct ccgggccaat ctggaccttc gcaacatcac gcagataaac
    35041 gggcacgcca ttttctctgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag
    35101 atagccgctg gcgcgcacca tatattccgc ttccgccagt tcgatcgacg aaccgcccgc
    35161 ttcctggttt gaagcatcca gcgcgctttt tacttcggct agactgatgc catattgcgc
    35221 caggcgctgg ggatcgataa ccacctgata ctctttcacc acgccgccca ccgacgccac
    35281 ttccgcaacg tcagggatgg tttttagctc atatttgaga aaccagtcct gcaatgagcg
    35341 taaatcggcc agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc
    35401 aacacccgtg gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac
    35461 ctggttgagg tactccagca cccgcgaacg cgcccagtac ggatcggtac catcttcgaa
    35521 aatgacatac acataagagt ctccaaactg cgagaaaccg cgcacagtct tcgcgccagg
    35581 caccgacaac atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc
    35641 ctgaccggga tagctggttt taataatcac ctgcacatcg gagagatccg gcagcgcatc
    35701 cactggcgtg ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag
    35761 caccaaaaaa cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct
    35821 caatgcgcat tggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct
    35881 tcggaatcaa tcaggaacag gccgctggaa accacctttt caccttccgc cagaccagag
    35941 tgtaacgcgg tgacgccttg cgatgcctgg aaaacggcaa cgcgtttcgg tacaaagcgt
    36001 ccgtcggcat caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac
    36061 ggaatgagca gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt
    36121 tttagcgcct cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca
    36181 tccacgccag gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga
    36241 acggtgagcg taaactgcga ggcatctttc accagccagg cgatagactc cgggatcgca
    36301 gcagtgaccc acaccgggtc cataccctga attttcgcta ccacgttatc tttggcgata
    36361 ttcattcccg cgcgcagatc aaacgcggtg atcacgccat caatgggtgc tttgagcgta
    36421 aagcgagtct ggattttttg cgtggcgatc aggcggcgaa tatccgcctc cggcattccc
    36481 gccagtcgca gccgctcaag aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc
    36541 agcagtaaat actcactctg cgcttccacc cagtcaggaa tggtcaggtc gagaagcggt
    36601 gtgccctttt gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggca
    36661 gcgcgagcct gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc
    36721 tgggcaaaag tcagcggtcc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc
    36781 tgagtcgggt caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc
    36841 agatccatat ccataaacgg cgatttacct ggtttatcga accgcgtatt ggggtacatc
    36901 gggtcgtacc agaataagat tttacgttct gcggtcgacg ttttttctgc gggtggttca
    36961 gcctttgcaa accaggtaaa acctgccgca gaaataatac cgcccgcgat catgctgccg
    37021 ataataagcg cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc
    37081 tgtaataaag aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta
    37141 atttcactca ttttcgtctg cggggtgatg gtaaagcgca tagtcatctc cggccagttc
    37201 acggcagcaa tcggatcgtg atggatggtg atttttttgc tttccagatc aatacccttt
    37261 accacgccag tggcgctaat aacctgcggt tgtacttcgc tcatggtttc atgatgatgt
    37321 tcgttagcct gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt
    37381 gcttttttca tttattctct cctggagtta aaaattattg ctgccaaccg ccccctaagg
    37441 cggtatacaa agaaatttcg ttaacctgac gggcataatt caggtcgagt aaagtttgtc
    37501 gggttgcaaa taaagaacgc tcggcatcca gcacttccag ataacttacc gcgccgtgct
    37561 gatataatgc ccgcgcccgt tgcaaagtaa tttgcagcga cgccagataa cgctgctggg
    37621 cgctgatttg atcgttcagg ctttgacgta atgccagcgc atctgccact tctttaaagg
    37681 cgttctggat tttctgttca taattcacta ccgactgctg ctggcgaatt tcggcgatat
    37741 ccagattggc ctggttgcgt ccggcattaa aaatggggat ctcaatttta ggaataaaat
    37801 tccacatccc gctgctagcg ttaaataatg aagacagatc gctgctggcg gttgatattc
    37861 cgctggtcag gctgatagac gggaaaaatg ccgcacgcgc agcaccaata ttagcattag
    37921 ccgccattaa cgcgtgttca gcttccataa tatccgggcg ctgcaataag atttgcgacg
    37981 acaagcccgc cggtaattta acgctttgca ggctgccgct gtttactgtc tgcgcttgcg
    38041 gcagtttgcc gtagcttccc aataacagtt gcaatgcatt attcgcctgc gccagttccc
    38101 cctgacgttt agcgatgtca ctgcgggtac tttctatcac cccgcgagcc tgttccagcg
    38161 ccagaacatt gctgctaccg gtcaacagtt gtttttcgac aaacgcatat gactgctgat
    38221 aattacgcag cgtttcttcg gctatttgca attgcgcata cgccagttgc tgattgaaat
    38281 agctttgcgc gacattagaa accagcagaa tatgcaccgc gcgctgagct tcctcagtgg
    38341 ctaaataatt ttgtcgctcg gcttcgctca tgttctttaa gcgaccgaaa aaatcgaggt
    38401 caaagctggc gttaaggccg gtcgaaaact cccgtgtcgt ggcggtattc cctttaagat
    38461 taccgctcca gctgccgctg ccctcgccat tgagctgtgg gtagcggtcg gcatcggtca
    38521 gacgatattg cgcccgtgcc tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat
    38581 tcaccagcgc ctcgctaatc agcgtcttca cctgattatc aacaaaaaag gtgcgccagc
    38641 ccacgttctg gtagttatct gctgcgttaa ccaagccgtt ctggctgagt gagaactgct
    38701 gcggcacggg cattgccgta cgctgataat ccggtgccag tgaacaaccg gttagcgcaa
    38761 gggccacaca aaatggcaga agtttacaag gagacatagg ctcataattt ctggtgattt
    38821 tataccgcca actttactcg ccaggctctg attttccggt gacaggaaaa tgacaaaatt
    38881 gtcattttgc caataagcga ttgccatctg atcccgctac tctagaattg cccgggcaac
    38941 atgcggagga aaaatgaaac tgttgattgt cgaagatgaa aagaaaaccg gagaatactt
    39001 gaccaaaggg ttaaccgaag ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg
    39061 ctaccatctg gcgatgaccg gtgattatga cctgataatc ctcgatatta tgctgccgga
    39121 cgtgaacggc tgggatatcg tgcgcatgtt gcgctccgcc aataaaggga tgccgattct
    39181 gttgcttacc gcgcttggca ccattgaaca tcgcgtcaag gggctggagt tgggggccga
    39241 tgactatctg gtgaagccgt tcgcttttgc tgaactgctg gcgcgggtgc gcactctcct
    39301 gcggcgcggg gcggcggtga ttatcgaaag tcagtttcag gttgccgatc tgatggtcga
    39361 tctcgtcagc cgcaaagtca cccgcagcgg cacgcgcatc actctgacca gtaaagagtt
    39421 tactctgctg gagttttttc tccgccatca gggcgaagtg ctgccccgct cgcttatcgc
    39481 ctcgcaggtg tgggacatga atttcgacag cgacactaac gccattgatg tggcggtgaa
    39541 gcggctacgc ggcaaaatcg acaacgactt tgagccgaag ctgattcaga ccgtgcgcgg
    39601 cgtgggctat atgcttgagg tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc
    39661 tggcaacccg tctgaccttt tttatcagcc tggccaccat cgcggcgttt ttcgcctttg
    39721 catggatcat gatccactca gtgaaagtgc attttgccga gcaggatatt aatgatttaa
    39781 aagaaattag cgccaccctt gaacgggtac ttaatcatcc tgacgagacg caagcccgac
    39841 gcttaatgac gctggaagat atcgtcagtg gttattccaa cgtgttgatt tccctggcag
    39901 atagccacgg caaaacggtg tatcactccc ccggtgcgcc ggatatccgc gagtttacgc
    39961 gtgacgccat acccgataaa gacgctcagg gtggcgaggt gtatctcctt tccggcccga
    40021 cgattatgat gccaggccac ggtcacgggc atatggaaca cagcaactgg cggatgatta
    40081 acttgccggt tggcccgttg gtggacggca aaccgattta tacgctctac atcgcgcttt
    40141 cgatcgattt tcatcttcat tacataaatg atttgatgaa taaacttatt atgaccgcat
    40201 ctgtcatcag catcctgatc gtctttatcg tgctattggc ggtacataaa ggtcacgcgc
    40261 cgatccgcag cgtcagccgt caaatccaga atatcacctc gaaagatctc gacgttcgcc
    40321 ttgacccgca gaccgagccc attgagctgg aacagctggt actgtcgttc aaccatatga
    40381 tcgagcgtat tgaggatgtc tttacccgcc agtccaattt ctcagcggat atcgctcacg
    40441 aaattcgcac gccgattacc aatctcatta cgcaaacgga aatcgccctc agccagtctc
    40501 gcagccagaa ggagctggaa gatgtgctct actctaatct cgaagagctg acgcgaatgg
    40561 cgaaaatggt cagcgatatg ctgtttctcg ctcaggccga taacaaccag ctaatccccg
    40621 aaaagaaaat gctcaacctg gcggatgaag tcggcaaagt gttcgatttt ttcgaggcgt
    40681 tagcggaaga tcgcggcgtg gagttgcgat ttgttggcga caaatgtcag gttgcgggcg
    40741 atccgctgat gctgcgtcgg gcgttaagca acctgctttc taatgccctg cgttatacgc
    40801 caaccagaga gacaattgta gtgcgctgtc agacggtcga tcaccaggtg caagttaccg
    40861 tcgaaaaccc cggtacgccc attgcgcccg agcacttacc gcgattgttt gaccgtttct
    40921 accgcgttga cccttcccgc cagcgaaaag gcgaaggtag cggcattggg ctggcgatag
    40981 tgaaatcgat tgttgtcgcg cataaaggca cggttgcagt aacgtcagat gcgcggggga
    41041 caaggtttgt gattatgctg ccggagagag agtgattatt aagttcaaat tcagggcgtg
    41101 gtgcatcacc tcctggtatt gttgatcaac aaattagaga aaaacttatt aaacatatga
    41161 aatatcgaag gcttttattt ttcaaagttt cacgagatgt ttgtatatga aacttagtta
    41221 aatctgtgtt tcttccaatt gtagatcaca atgccctttc taaattttca tcgttataaa
    41281 caagccatct atcatataaa gcaatgaata atttaatcgc gattaatcat cattaattgt
    41341 tatattattc actcaatcta tttgtcaagt gaatttgata aattcctaat ttaaaatatc
    41401 cactattgag ccagtattta acaacgttaa gatgtgcaat gtgcagatca tgttatcagg
    41461 gtggagcggt agcatatatt tcttatgcaa tcattcattt taataatgtt aattggatta
    41521 tttattgctg tgaataaaaa tatattatga atattggttt aactttgcgt ttttgttact
    41581 cgttgtgata tgcgtacctg tcctgaataa cgccaagaaa caagaggttg agcttgtgat
    41641 tatttttttg actgaacaga cacccgtttc tgtaaagtaa tatttaaaca aaattctcac
    41701 ataaccatag cattttctgt tccttcccta ataacgtctg aagtcgtccc gcttatcaca
    41761 ttatccgcag ttgtacggta cggatcatta ttcattttac tggaggttgc tatgtcaacc
    41821 ggattacgtt tcacactgga agtggacggc ctgccgccgg acgcttttgc ggtggtttcc
    41881 tttcatctga accagtcact ctcttcgctc ttttccctcg atctctccct ggtcagccag
    41941 cagtttctct cccttgaatt tgcgcaggtg ctggacaaaa tggcctacct gacgatatgg
    42001 cagggcgatg aagtacagcg ccgggtgaaa ggcgtggtga cctggtttga actcggggag
    42061 aacgacaaaa accagatgct gtacagcatg aaggtgcacc cgccgctgtg gcgtgccggt
    42121 ctgcgccaga acttccgtat cttccagaac gaggacatca aaagcatcct cggcacgatg
    42181 ttgcaggaaa acggggtgac cgaatggagt ccgctgttca gcgagccgca tccttcccgt
    42241 gagttttgtg tccagtacgg tgagactgat tacgatttcc tgtgccggat ggcggcggag
    42301 gaaggcatct tcttttatga ggagcatgct tacaaaagta ccgaccagag cctggtgctg
    42361 tgcgacacag tccgccatct gcccgaatct tttgaaatcc catggaaccc gaacacccgt
    42421 accgaggtga gcaccctctg catcagccag ttccgctaca gcgcacaaat ccgcccttct
    42481 tccgtggtga ccaaagacta cacctttaaa cgccccggct gggccggacg ttttgaacag
    42541 gaaggccagc accaggacta ccagcgcacg cagtatgaag tgtatgacta ccccggacgt
    42601 ttcaagggtg ctcacgggca gaactttgcc cgctggcaga tggacggctg gcgaaacaat
    42661 gcagaaaccg cgcggggaat gagtcgctcg ccggagatat ggccgggacg acgaattgtg
    42721 ctgacggggc atccgcaggc gaacctgaac cgggaatggc aggtggtggc aagtgaactg
    42781 cacggcgaac agccacaggc ggtgccagga cggcagggag cggggacggc gctggagaac
    42841 cattttgcgg tgatcccggc agacagaaca tggcgaccac agccgttgct gaaaccgctg
    42901 gtcgacggcc cgcagagcgc tgtcgtgaca ggaccggcag gcgaggaaat cttctgcgac
    42961 gaacatggtc gcgtgcgggt gaagttcaac tgggaccgtt ataacccggc agaccaagac
    43021 agttcgtgct ggatccgtgt ggcacaggcg tgggcaggca ccggttttgg ccacctggcg
    43081 ataccgcgtg tgggtcagga agtgattgta gacttcctca acggcgatcc ggaccagccg
    43141 atcattatgg ggcgcaccta ccaccaggaa aaccgccccc ccggcagcct gccgggaaca
    43201 aagacgcaga tgaccatccg ctccaaaacg tacatgggca gcggatttaa tgagctgaag
    43261 tttgatgatg cgacagggag agaacaggtc tacatccacg cgcagaagaa catggatacc
    43321 gaagtgctca acgaccgtac caccaccgta aaacacgatc accgcgaaac cgtaaaaaat
    43381 gaccagacgg tcacgatcca ggaaggtaac cgccttctta cggtggaaaa aggccacaag
    43441 atcaccggag tactgaaagg gtctttatct gaggatgtct ttcaggacag aggcacgatt
    43501 gccggttcgg tgcatgttga cgctgtaaac aatggtggcg aaggcgacgg tatacaggct
    43561 tatacggcga ttaaggaaat tttgctggcc gtggaggaaa gcaaaattgc gctgacgccg
    43621 gatagcattc agctacaggt cggggaatcg acggtaatca ggctgtcgaa ggatggcatc
    43681 accatcgtgg gcggttctgt tttcatcaac tgattcactc agattatcag ggagtatttt
    43741 atgcagttca cttttaatga aggccacatt caactgccgt ctcaatggca ggatcagtca
    43801 atgcaggtcc tggtgtccac agataacagt ggcatcaatc tggtcattac ccgcgaagcc
    43861 gttccacagg gaaccctgac accagagctg taccaggaaa cgctggcgct gtaccagggc
    43921 aaacttgatg ggtacaccga acatgcctgc cgggaaatca ctctggcaga ggccccggcc
    43981 tggctgctgg attattcctg gaacggtccg gaagatgagg ggaatcaggg gcggattagc
    44041 cagatagccg tattccagcg gcgtggagat acgctgctga ccttcacttt cagcacctcc
    44101 ttatccctga agaacagtca aaagacgatg ctgctggagg tgataaagag cttcaccccg
    44161 ttgccaccgg agaatgacat ccaaaaggac cagccgcgct gacataaacg agggataaca
    44221 catgagtgaa ggaccaggcg ggccacaggg agcgaccgca ggcggtacgc tggcaatgcg
    44281 aatgctgtca cagcaggcga tggtcgccag ccagatgaaa cgggcagcca acgacaaagc
    44341 cattgcacag atgttggcat caaaaaagtc tggccccccc gccgccaggc tgggcgatga
    44401 aattcagcac aagagttttt tgggggcgct ggcaggggcc gtactggggg cgatagtgac
    44461 catcgcagaa ggttgcctga ttatggccgc ctgtgccacc ggcccttatg cgctggttct
    44521 ggtacctgcg ctgatgtatg ccagctataa ggcgagtgat tatgtggagg agaaacagaa
    44581 ccagcttgaa tcatggataa acagcttttg tgacacggac ggcgccatca ataccggttc
    44641 tgaaaacgta aaaattaacg gcgagctggc cgcacgtgca gcggtcaccc ttccccctcc
    44701 tcccccacct ggagcaatac ctgaagtccc acagggggaa ccctcatggg gtgatattgc
    44761 cactgacctg cttgaatcgg cagcggaaaa agcagtacca ctggcgaagg cctgggggaa
    44821 cgctgttatc accctgacgg acagcaatgc cggttttatg gatcgcgtca gcgccggcgc
    44881 atcgcttctg tttcccgccg gtccggtatt aatggagttt gccaccatgg tgggcgggcg
    44941 tggcgaaatc aaaaaagatg tggatttccc ggaagccggt gaggacacgg cgctctgcga
    45001 caaggagaac aaaccaccga ggatagccca gggcagcagc aacgtcttta tcaacaatca
    45061 gcctgccgcg cgcaagggcg acaaactgga gtgcagtgcg gcgatcgtgg gaggttcgcc
    45121 ggacgtcttt attggcggtg agcaggtcac atatctggat atccagccgg agttcccgcc
    45181 gtggcagaga atgatcctgg ggggaataac gatagccagc tatcttctgc cgccagcggg
    45241 actactggga aaactgaaga atctggcgag actcggcaaa ctgggaaact gctggggaaa
    45301 agcgggaagc tgctgggcgc aaagctcggc gcgttgctgg ggaaaacagg taagtcgtta
    45361 aaaagtattg ccaataaagt catcagatgg gtaacagatc ctgtcgatcc ggtaaccggc
    45421 gcatactgcg acgaacgtac cgacttcacc ctgggccaga ccctccccct ctccttcacc
    45481 cgtttccaca gttctgtact gccgctgcat ggcctgaccg gcgtgggctg gagcgactcc
    45541 tggagcgaat acgcctgggt gcgtgaacag ggaaaccggg tggatatcat cagccaggga
    45601 gccacgctga gatttgcctt cgacggtgac agtgatacgg cggttaaccc gtatcacgcc
    45661 cagtacattc tgcgccgccg cgatgattat ctggagctgt tcgacaggga tgcactgagc
    45721 agccgcttct tttatgacgc ctttccggga atgcgtctgc gccacccggt gactgacgat
    45781 accagcgatg accgcctggc acacagcccc aatgaccgga tgtacatgct gggcgggatg
    45841 agcgacaccg ccagcaaccg catcacgttt gagcgcgaca gccagtaccg gatcacgggt
    45901 gtcagtcaca ccgacgggat ccggcttaaa ctgacgtacc acgccagcgg ctacctgaaa
    45961 gccattcacc gcacggataa cgacatacag acgctggcga cctacgaaca ggatgcgcgg
    46021 gggcggctga cagaagcgga tgcgcggctg gactaccacc tgttttatga gtacgacgct
    46081 gcggaccgga tcatccgctg gtccgataac gaccagacgt ggagccgttt cacctacgat
    46141 gaacagggcc ggtgcgtgaa tgtcaccggg gcggagggct attacaacgc cacgctggac
    46201 tatggtgacg gctgcaccac cgtgacggac ggcaagggca ttcaccgtta ttactatgat
    46261 cctgacggca atattctgcg ggaagaagcg ccggacggca gcaccaccat gtatgaatgg
    46321 gatgaattcc atcacctgct ggcccgccac tcccctgccg ggcgggtgga gaaatttgaa
    46381 tacaacgccg cacacggtca gttaagccgt tacacggcgg cagacggcgc ggagtggcag
    46441 taccgctatg atgagcgcgg cctgctcagc aacatcaccg accctgccgg gcagacgtgg
    46501 acacagcagt gtgatgaacg cggcctgccg gtaagtctgg tgtcgccaca gggcgaagag
    46561 acccggctgg catacaccgc tcaggggctg ctgtcgggaa tattccgcca ggatgaacgg
    46621 cgtctgggca tagagtacga ccaccacaac cggccggaaa cactcaccga cgtgatgggc
    46681 cgtgaacacc acaccgaata cagcggtcac gacctgccag tgaagatgcg cggccccggc
    46741 ggtcagtcag tgcggttaca gtggcagcag caccataaac tgagcggcat tgaacgtgct
    46801 ggaaccggcg cggaaggatt tcgctatgac cgccacggca acctgctggc ctggacggac
    46861 ggcaacggtg ttgtctggac gatggaatac ggtccgttcg attttccggt ggcgcgaacg
    46921 gacggtgaag gccaccgctg gcagtaccgc tacgataaag acacgctgca actgacagaa
    46981 gtcatcaacc cgcagggtga gtcatatctt tatgttctgg ataactgcgg ccgggtgacg
    47041 gaagagcgtg actggggcgg cgtggtctgg cgttaccgct atgacgccga tggcctctgt
    47101 accgccaagg ttaacggcct ggaggaaacc atcctctaca gccgggacgc cgcaggccgc
    47161 ctggcagaaa tcatctcgcc ggaaggcaaa acgcagtatg cctatgacaa atccggcagg
    47221 ctgacgggta tcttcagccc ggacggcaca tcacagcgca ccggctatga cgaacgcggg
    47281 cgggtgaatg tcaccactca gggccgacgg gccattgaat acaactaccc cgatgaacac
    47341 accgtcatcc gctgtatcct gccaccggaa gatgaacgcg acagacaccc cggtgaatcc
    47401 ctgctgaaaa ccacgtaccg ctacaacgcc gccggagaac tgacagaggt cattctgccg
    47461 ggggatgaga cgctgacgtt cagccgtgat gaggcgggac gtgaagtgct ccggcacagt
    47521 aaccggggtt ttgcctgtga acagggctgg aatgcagccg gtcagcctgt cagtcagcgc
    47581 gccggatttt ttccggcaga agccacatgg gacgggctgg ttccctcact ggtacgggag
    47641 taccgttacg acagcgcggg taacgtgtca ggcgtcacca gccgggaaga ttacggacgg
    47701 gaaacacggc gggagtatcg gctggaccgg aacggccagg tcacggcggt gacagcctca
    47761 ggcaccgggc tgggctatgg cgaaggcgat gagtcctatg gctatgacag ctgcggctac
    47821 ctgaaggcgc agtctgcggg caggcaccgg ataagtgaag agactgacca gtatgccgga
    47881 ggccaccggc tgaaacaggc cggaaacacg cagtatgact atgacgccgc aggccggatg
    47941 gtcagccgca ccagacaccg tgacggctac cgaccggaaa cagagcggtt ccggtgggac
    48001 agccgggacc agctgaccgg gtattgcagc gcacagggtg agctgtggga ataccgccac
    48061 gacgccagcg gcagacgaac agagaaacgc tgcgaccgga agaaaatccg cttcacgtat
    48121 ctgtgggacg gcgacagtat tgcggaaatc cgggaatacc gcgatgataa actgtacagc
    48181 gtaaggcacc tggtgtttaa cggctttgag ctgataagcc agcagttcag ccgggtacgg
    48241 caggcgcatc cgtcggtggc cccgcagtgg gtgacgcgga cgaatcatgc ggtgagcgac
    48301 ctgacgggcc gcccgctgat gctctttaac agtgaaggaa agacggtctg gcgaccgggg
    48361 cagaccagcc tgtgggggct ggcactcagc ctgcccgcag acaccggcta cccggacccg
    48421 cgcggggaac tggacccgga agccgacccc ggcctgctgt atgcaggaca gtggcaggat
    48481 gcggaatccg ggctgtgcta taatcggttc cggtattacg agccggaaac cggaatgtac
    48541 ctggtgagtg atccgctggg gttgcagggc ggggagcaga cttaccggta tgtgccgaat
    48601 ccttgtgggt gggttgatcc gctgggattg gctgcaagtt ctaaaatcag cagtttgatg
    48661 gactatattg gcgatggtcg tcgtgttagt gggcatacgg gtttcctgga tggggttcgt
    48721 ttatcacgta gtcaaataa
//
LOCUS       DAJVXP010000012        46685 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVXP010000012 DAJVXP010000000
VERSION     DAJVXP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.012"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OQS10_000719"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OQS10_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS2291837.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="OQS10_000720"
     CDS             complement(280..873)
                     /locus_tag="OQS10_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS2291838.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="OQS10_000721"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="OQS10_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2291839.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="OQS10_000722"
     CDS             1788..2183
                     /locus_tag="OQS10_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS2291840.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="OQS10_000723"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="OQS10_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS2291841.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="OQS10_000724"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="OQS10_000724"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS2291842.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="OQS10_000725"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="OQS10_000725"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS2291843.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="OQS10_000726"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="OQS10_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS2291844.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OQS10_000727"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OQS10_000727"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS2291845.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OQS10_000728"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OQS10_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS2291846.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="OQS10_000729"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="OQS10_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS2291847.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="OQS10_000730"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="OQS10_000730"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS2291848.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="OQS10_000731"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="OQS10_000731"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS2291849.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="OQS10_000732"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="OQS10_000732"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS2291850.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="OQS10_000733"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="OQS10_000733"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS2291851.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OQS10_000734"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OQS10_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS2291852.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OQS10_000735"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OQS10_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS2291853.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="OQS10_000736"
     CDS             17158..17709
                     /locus_tag="OQS10_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS2291854.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OQS10_000737"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OQS10_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS2291855.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="OQS10_000738"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="OQS10_000738"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS2291856.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OQS10_000739"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OQS10_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS2291857.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHLIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="OQS10_000740"
     CDS             complement(21354..22376)
                     /locus_tag="OQS10_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2291858.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="OQS10_000741"
     CDS             complement(22390..23892)
                     /locus_tag="OQS10_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS2291859.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OQS10_000742"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OQS10_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS2291860.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="OQS10_000743"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="OQS10_000743"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS2291861.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="OQS10_000744"
     CDS             complement(26207..26548)
                     /locus_tag="OQS10_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS2291862.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OQS10_000745"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OQS10_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS2291863.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OQS10_000746"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OQS10_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS2291864.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="OQS10_000747"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="OQS10_000747"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS2291865.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="OQS10_000748"
     CDS             33227..34570
                     /locus_tag="OQS10_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS2291866.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="OQS10_000749"
     CDS             complement(34650..34856)
                     /locus_tag="OQS10_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS2291867.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="OQS10_000750"
     CDS             35181..35735
                     /locus_tag="OQS10_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS2291868.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="OQS10_000751"
     CDS             complement(35825..36736)
                     /locus_tag="OQS10_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291869.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OQS10_000752"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OQS10_000752"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS2291870.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="OQS10_000753"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="OQS10_000753"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS2291871.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="OQS10_000754"
     CDS             complement(39939..40319)
                     /locus_tag="OQS10_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS2291872.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="OQS10_000755"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="OQS10_000755"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS2291873.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="OQS10_000756"
     CDS             41376..42341
                     /locus_tag="OQS10_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS2291874.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="OQS10_000757"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="OQS10_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS2291875.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OQS10_000758"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OQS10_000758"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS2291876.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OQS10_000759"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OQS10_000759"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS2291877.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="OQS10_000760"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="OQS10_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCS2291878.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46685)
                     /locus_tag="OQS10_000761"
     CDS             complement(46338..>46685)
                     /locus_tag="OQS10_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS2291879.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT        11455 a        12415 c        11631 g        11184 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgagat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggtt
//
LOCUS       DAJVXP010000013        45202 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVXP010000013 DAJVXP010000000
VERSION     DAJVXP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.013"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(9..668)
                     /gene="pgmB"
                     /locus_tag="OQS10_000762"
     CDS             complement(9..668)
                     /gene="pgmB"
                     /locus_tag="OQS10_000762"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS2291880.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(665..2932)
                     /gene="ycjT"
                     /locus_tag="OQS10_000763"
     CDS             complement(665..2932)
                     /gene="ycjT"
                     /locus_tag="OQS10_000763"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS2291881.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(2929..3984)
                     /locus_tag="OQS10_000764"
     CDS             complement(2929..3984)
                     /locus_tag="OQS10_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2291882.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(3994..4782)
                     /locus_tag="OQS10_000765"
     CDS             complement(3994..4782)
                     /locus_tag="OQS10_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS2291883.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(4800..5852)
                     /locus_tag="OQS10_000766"
     CDS             complement(4800..5852)
                     /locus_tag="OQS10_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS2291884.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(5883..6486)
                     /locus_tag="OQS10_000767"
                     /pseudo
     CDS             complement(5883..6486)
                     /locus_tag="OQS10_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(6473..7354)
                     /locus_tag="OQS10_000768"
     CDS             complement(6473..7354)
                     /locus_tag="OQS10_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS2291885.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7375..8685)
                     /locus_tag="OQS10_000769"
     CDS             complement(7375..8685)
                     /locus_tag="OQS10_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2291886.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(8720..10230)
                     /locus_tag="OQS10_000770"
                     /pseudo
     CDS             complement(8720..10230)
                     /locus_tag="OQS10_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(10442..10756)
                     /gene="pspE"
                     /locus_tag="OQS10_000771"
     CDS             complement(10442..10756)
                     /gene="pspE"
                     /locus_tag="OQS10_000771"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS2291887.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(10831..11052)
                     /gene="pspD"
                     /locus_tag="OQS10_000772"
     CDS             complement(10831..11052)
                     /gene="pspD"
                     /locus_tag="OQS10_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS2291888.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11061..11420)
                     /gene="pspC"
                     /locus_tag="OQS10_000773"
     CDS             complement(11061..11420)
                     /gene="pspC"
                     /locus_tag="OQS10_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS2291889.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11420..11644)
                     /gene="pspB"
                     /locus_tag="OQS10_000774"
     CDS             complement(11420..11644)
                     /gene="pspB"
                     /locus_tag="OQS10_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS2291890.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(11698..12366)
                     /gene="pspA"
                     /locus_tag="OQS10_000775"
     CDS             complement(11698..12366)
                     /gene="pspA"
                     /locus_tag="OQS10_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS2291891.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12533..13510
                     /gene="pspF"
                     /locus_tag="OQS10_000776"
     CDS             12533..13510
                     /gene="pspF"
                     /locus_tag="OQS10_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS2291892.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13628..14893)
                     /gene="puuE"
                     /locus_tag="OQS10_000777"
                     /pseudo
     CDS             complement(13628..14893)
                     /gene="puuE"
                     /locus_tag="OQS10_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(14931..16211)
                     /gene="puuB"
                     /locus_tag="OQS10_000778"
     CDS             complement(14931..16211)
                     /gene="puuB"
                     /locus_tag="OQS10_000778"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS2291893.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16213..17703)
                     /gene="puuC"
                     /locus_tag="OQS10_000779"
                     /pseudo
     CDS             complement(16213..17703)
                     /gene="puuC"
                     /locus_tag="OQS10_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(17853..18410)
                     /gene="puuR"
                     /locus_tag="OQS10_000780"
     CDS             complement(17853..18410)
                     /gene="puuR"
                     /locus_tag="OQS10_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS2291894.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18437..19201)
                     /gene="puuD"
                     /locus_tag="OQS10_000781"
     CDS             complement(18437..19201)
                     /gene="puuD"
                     /locus_tag="OQS10_000781"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS2291895.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19413..20774
                     /gene="puuA"
                     /locus_tag="OQS10_000782"
     CDS             19413..20774
                     /gene="puuA"
                     /locus_tag="OQS10_000782"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS2291896.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            20923..21087
                     /locus_tag="OQS10_000783"
     CDS             20923..21087
                     /locus_tag="OQS10_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291897.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21077..22462
                     /gene="puuP"
                     /locus_tag="OQS10_000784"
     CDS             21077..22462
                     /gene="puuP"
                     /locus_tag="OQS10_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS2291898.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            22596..22841
                     /locus_tag="OQS10_000785"
     CDS             22596..22841
                     /locus_tag="OQS10_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS2291899.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23154..24797
                     /gene="sapA"
                     /locus_tag="OQS10_000786"
     CDS             23154..24797
                     /gene="sapA"
                     /locus_tag="OQS10_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS2291900.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            24794..25759
                     /gene="sapB"
                     /locus_tag="OQS10_000787"
     CDS             24794..25759
                     /gene="sapB"
                     /locus_tag="OQS10_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS2291901.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            25746..26636
                     /gene="sapC"
                     /locus_tag="OQS10_000788"
     CDS             25746..26636
                     /gene="sapC"
                     /locus_tag="OQS10_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS2291902.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            26636..27628
                     /gene="sapD"
                     /locus_tag="OQS10_000789"
     CDS             26636..27628
                     /gene="sapD"
                     /locus_tag="OQS10_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS2291903.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            27630..28436
                     /gene="sapF"
                     /locus_tag="OQS10_000790"
     CDS             27630..28436
                     /gene="sapF"
                     /locus_tag="OQS10_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS2291904.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            28504..28857
                     /locus_tag="OQS10_000791"
     CDS             28504..28857
                     /locus_tag="OQS10_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS2291905.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29225..30013
                     /gene="fabI"
                     /locus_tag="OQS10_000792"
     CDS             29225..30013
                     /gene="fabI"
                     /locus_tag="OQS10_000792"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS2291906.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30158..31284
                     /locus_tag="OQS10_000793"
                     /pseudo
     CDS             30158..31284
                     /locus_tag="OQS10_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            31352..33286
                     /gene="rnb"
                     /locus_tag="OQS10_000794"
     CDS             31352..33286
                     /gene="rnb"
                     /locus_tag="OQS10_000794"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS2291907.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(33364..33467)
                     /locus_tag="OQS10_000795"
                     /pseudo
     CDS             complement(33364..33467)
                     /locus_tag="OQS10_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33521..35511
                     /gene="pdeR"
                     /locus_tag="OQS10_000796"
                     /pseudo
     CDS             33521..35511
                     /gene="pdeR"
                     /locus_tag="OQS10_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            35658..35831
                     /locus_tag="OQS10_000797"
     CDS             35658..35831
                     /locus_tag="OQS10_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291908.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            35921..36670
                     /locus_tag="OQS10_000798"
     CDS             35921..36670
                     /locus_tag="OQS10_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2291909.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            36939..37157
                     /gene="osmB"
                     /locus_tag="OQS10_000799"
     CDS             36939..37157
                     /gene="osmB"
                     /locus_tag="OQS10_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS2291910.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37283..37609)
                     /gene="yciH"
                     /locus_tag="OQS10_000800"
     CDS             complement(37283..37609)
                     /gene="yciH"
                     /locus_tag="OQS10_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS2291911.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(37609..38346)
                     /gene="pyrF"
                     /locus_tag="OQS10_000801"
     CDS             complement(37609..38346)
                     /gene="pyrF"
                     /locus_tag="OQS10_000801"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS2291912.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(38539..39708)
                     /gene="lapB"
                     /locus_tag="OQS10_000802"
     CDS             complement(38539..39708)
                     /gene="lapB"
                     /locus_tag="OQS10_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS2291913.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(39715..40023)
                     /gene="lapA"
                     /locus_tag="OQS10_000803"
     CDS             complement(39715..40023)
                     /gene="lapA"
                     /locus_tag="OQS10_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS2291914.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40172..40936)
                     /gene="pgpB"
                     /locus_tag="OQS10_000804"
     CDS             complement(40172..40936)
                     /gene="pgpB"
                     /locus_tag="OQS10_000804"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS2291915.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41106..41696
                     /gene="ribA"
                     /locus_tag="OQS10_000805"
     CDS             41106..41696
                     /gene="ribA"
                     /locus_tag="OQS10_000805"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS2291916.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(41760..44435)
                     /gene="acnA"
                     /locus_tag="OQS10_000806"
     CDS             complement(41760..44435)
                     /gene="acnA"
                     /locus_tag="OQS10_000806"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS2291917.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(44599..44694)
                     /locus_tag="OQS10_000807"
     CDS             complement(44599..44694)
                     /locus_tag="OQS10_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291918.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(44808..44975)
                     /locus_tag="OQS10_000808"
     CDS             complement(44808..44975)
                     /locus_tag="OQS10_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291919.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(44978..45142)
                     /locus_tag="OQS10_000809"
     CDS             complement(44978..45142)
                     /locus_tag="OQS10_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS2291920.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10689 a        11929 c        11391 g        11193 t
ORIGIN      
        1 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
       61 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      121 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      181 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      241 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
      301 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
      361 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
      421 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
      481 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
      541 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
      601 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
      661 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
      721 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
      781 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
      841 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
      901 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
      961 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1021 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1081 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1141 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1201 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1261 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     1321 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     1381 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     1441 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     1501 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     1561 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     1621 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     1681 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     1741 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     1801 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     1861 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     1921 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     1981 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2041 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2101 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2161 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2221 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     2281 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     2341 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     2401 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     2461 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     2521 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     2581 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     2641 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     2701 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     2761 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     2821 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     2881 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     2941 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3001 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3061 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3121 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3181 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3241 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     3301 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     3361 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     3421 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     3481 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     3541 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     3601 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     3661 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     3721 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     3781 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     3841 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     3901 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     3961 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4021 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4081 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4141 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4201 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4261 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     4321 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     4381 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     4441 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     4501 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     4561 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     4621 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     4681 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     4741 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     4801 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     4861 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     4921 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     4981 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5041 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5101 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5161 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5221 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     5281 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     5341 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     5401 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     5461 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     5521 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     5581 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     5641 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     5701 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     5761 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     5821 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     5881 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     5941 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6001 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6061 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6121 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6181 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6241 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     6301 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     6361 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     6421 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     6481 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     6541 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     6601 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     6661 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     6721 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     6781 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     6841 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     6901 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     6961 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7021 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7081 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7141 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7201 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7261 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     7321 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     7381 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     7441 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     7501 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     7561 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     7621 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     7681 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     7741 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     7801 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     7861 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     7921 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     7981 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8041 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8101 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     8161 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8221 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     8281 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     8341 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     8401 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     8461 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     8521 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     8581 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     8641 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     8701 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     8761 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     8821 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     8881 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     8941 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9001 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9061 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9121 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9181 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9241 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
     9301 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
     9361 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
     9421 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
     9481 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
     9541 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
     9601 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
     9661 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
     9721 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
     9781 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
     9841 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
     9901 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
     9961 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10021 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10081 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10141 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10201 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10261 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    10321 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    10381 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    10441 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    10501 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    10561 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    10621 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    10681 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    10741 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    10801 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    10861 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    10921 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    10981 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11041 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11101 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11161 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccagc
    11221 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    11281 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    11341 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    11401 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    11461 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    11521 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    11581 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    11641 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    11701 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    11761 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    11821 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    11881 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    11941 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12001 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12061 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12121 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12181 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12241 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    12301 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    12361 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    12421 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    12481 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    12541 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    12601 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    12661 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    12721 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    12781 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    12841 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    12901 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    12961 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13021 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13081 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13141 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13201 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13261 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    13321 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    13381 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    13441 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    13501 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    13561 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    13621 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    13681 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    13741 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    13801 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    13861 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    13921 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    13981 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14041 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14101 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14161 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14221 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    14281 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    14341 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    14401 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    14461 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    14521 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    14581 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    14641 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    14701 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    14761 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    14821 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    14881 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    14941 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15001 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15061 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15121 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15181 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15241 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    15301 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    15361 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    15421 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    15481 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    15541 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    15601 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    15661 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    15721 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    15781 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    15841 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    15901 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    15961 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16021 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16081 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16141 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16201 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16261 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    16321 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    16381 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    16441 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    16501 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    16561 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    16621 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    16681 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    16741 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    16801 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    16861 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    16921 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    16981 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17041 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17101 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17161 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17221 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    17281 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    17341 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    17401 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    17461 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    17521 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    17581 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    17641 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    17701 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    17761 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    17821 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    17881 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    17941 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18001 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18061 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18121 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18181 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18241 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    18301 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    18361 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    18421 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    18481 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    18541 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    18601 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    18661 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    18721 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    18781 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    18841 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    18901 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    18961 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19021 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19081 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19141 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19201 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19261 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    19321 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    19381 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    19441 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    19501 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    19561 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    19621 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    19681 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    19741 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    19801 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    19861 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    19921 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    19981 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20041 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20101 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20161 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20221 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    20281 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    20341 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    20401 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    20461 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    20521 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    20581 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    20641 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    20701 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    20761 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    20821 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    20881 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    20941 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21001 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21061 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21121 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21181 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21241 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    21301 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    21361 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    21421 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    21481 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    21541 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    21601 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    21661 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    21721 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    21781 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    21841 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    21901 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    21961 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22021 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22081 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22141 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22201 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22261 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    22321 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    22381 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    22441 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    22501 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    22561 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    22621 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    22681 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    22741 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    22801 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    22861 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    22921 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    22981 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23041 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23101 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23161 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23221 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    23281 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    23341 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    23401 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    23461 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    23521 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    23581 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    23641 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    23701 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    23761 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    23821 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    23881 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    23941 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24001 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24061 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24121 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24181 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24241 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    24301 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    24361 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    24421 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    24481 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    24541 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    24601 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    24661 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    24721 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    24781 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    24841 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    24901 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    24961 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25021 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25081 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25141 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25201 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25261 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    25321 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    25381 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    25441 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    25501 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    25561 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    25621 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    25681 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    25741 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    25801 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    25861 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    25921 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    25981 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26041 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26101 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26161 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26221 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    26281 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    26341 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    26401 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    26461 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    26521 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    26581 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    26641 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    26701 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    26761 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    26821 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    26881 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    26941 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27001 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27061 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27121 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27181 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27241 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    27301 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    27361 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    27421 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    27481 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    27541 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    27601 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    27661 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    27721 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    27781 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    27841 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    27901 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    27961 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28021 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28081 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28141 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28201 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28261 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    28321 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    28381 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    28441 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    28501 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    28561 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    28621 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    28681 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    28741 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    28801 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    28861 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    28921 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    28981 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29041 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29101 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29161 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29221 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    29281 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    29341 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    29401 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    29461 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    29521 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    29581 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    29641 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    29701 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    29761 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    29821 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    29881 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    29941 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30001 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30061 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30121 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30181 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30241 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    30301 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    30361 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    30421 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    30481 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    30541 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    30601 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    30661 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    30721 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    30781 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    30841 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    30901 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    30961 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31021 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31081 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31141 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31201 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31261 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    31321 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    31381 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    31441 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    31501 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    31561 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    31621 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    31681 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    31741 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    31801 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    31861 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    31921 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    31981 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32041 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32101 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32161 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32221 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    32281 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    32341 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    32401 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    32461 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    32521 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    32581 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    32641 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    32701 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    32761 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    32821 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    32881 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    32941 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33001 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33061 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33121 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33181 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33241 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    33301 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    33361 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    33421 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    33481 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    33541 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    33601 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    33661 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    33721 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    33781 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    33841 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    33901 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    33961 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34021 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34081 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34141 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34201 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34261 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    34321 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    34381 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    34441 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    34501 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    34561 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    34621 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    34681 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    34741 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    34801 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    34861 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    34921 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    34981 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35041 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35101 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35161 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35221 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    35281 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    35341 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    35401 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    35461 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    35521 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    35581 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    35641 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    35701 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    35761 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    35821 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    35881 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    35941 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36001 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36061 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36121 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    36181 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36241 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    36301 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    36361 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    36421 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    36481 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    36541 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    36601 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    36661 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    36721 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    36781 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    36841 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    36901 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    36961 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37021 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37081 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37141 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37201 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37261 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    37321 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    37381 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    37441 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    37501 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    37561 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    37621 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    37681 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    37741 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    37801 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    37861 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    37921 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    37981 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38041 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38101 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38161 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38221 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    38281 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    38341 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    38401 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    38461 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    38521 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    38581 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    38641 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    38701 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    38761 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    38821 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    38881 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    38941 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39001 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39061 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39121 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39181 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39241 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    39301 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    39361 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    39421 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    39481 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    39541 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    39601 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    39661 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    39721 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    39781 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    39841 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    39901 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    39961 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40021 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40081 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40141 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40201 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40261 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    40321 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    40381 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    40441 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    40501 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    40561 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    40621 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    40681 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    40741 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    40801 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    40861 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    40921 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    40981 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41041 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41101 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41161 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41221 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    41281 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    41341 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    41401 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    41461 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    41521 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    41581 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    41641 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    41701 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    41761 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    41821 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    41881 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    41941 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42001 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42061 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42121 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42181 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42241 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    42301 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    42361 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    42421 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    42481 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    42541 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    42601 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    42661 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    42721 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    42781 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    42841 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    42901 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    42961 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43021 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43081 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43141 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43201 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43261 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    43321 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    43381 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    43441 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    43501 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    43561 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    43621 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    43681 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    43741 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    43801 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    43861 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    43921 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    43981 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44041 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44101 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44161 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44221 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    44281 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    44341 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    44401 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    44461 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    44521 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    44581 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    44641 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    44701 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    44761 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    44821 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    44881 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    44941 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45001 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45061 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45121 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45181 caaatcctaa agaaacttta gt
//
LOCUS       DAJVXP010000014        43714 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVXP010000014 DAJVXP010000000
VERSION     DAJVXP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.014"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..559)
                     /locus_tag="OQS10_000810"
     CDS             complement(<1..559)
                     /locus_tag="OQS10_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS2291921.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="OQS10_000811"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="OQS10_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS2291922.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OQS10_000812"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OQS10_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS2291923.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OQS10_000813"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OQS10_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS2291924.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="OQS10_000814"
     CDS             complement(4001..6322)
                     /locus_tag="OQS10_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS2291925.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OQS10_000815"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OQS10_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS2291926.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="OQS10_000816"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="OQS10_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS2291927.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="OQS10_000817"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="OQS10_000817"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS2291928.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OQS10_000818"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OQS10_000818"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS2291929.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="OQS10_000819"
     CDS             11268..11582
                     /locus_tag="OQS10_000819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS2291930.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="OQS10_000820"
     CDS             11595..12968
                     /locus_tag="OQS10_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS2291931.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OQS10_000821"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OQS10_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS2291932.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OQS10_000822"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OQS10_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS2291933.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OQS10_000823"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OQS10_000823"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS2291934.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OQS10_000824"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OQS10_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS2291935.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="OQS10_000825"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="OQS10_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS2291936.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OQS10_000826"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OQS10_000826"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS2291937.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="OQS10_000827"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="OQS10_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS2291938.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="OQS10_000828"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="OQS10_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="OQS10_000829"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="OQS10_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS2291939.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="OQS10_000830"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="OQS10_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS2291940.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="OQS10_000831"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="OQS10_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS2291941.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="OQS10_000832"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="OQS10_000832"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS2291942.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="OQS10_000833"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="OQS10_000833"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS2291943.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="OQS10_000834"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="OQS10_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS2291944.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="OQS10_000835"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="OQS10_000835"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS2291945.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="OQS10_000836"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="OQS10_000836"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS2291946.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="OQS10_000837"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="OQS10_000837"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS2291947.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="OQS10_000838"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="OQS10_000838"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS2291948.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="OQS10_000839"
     CDS             31280..32185
                     /locus_tag="OQS10_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291949.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="OQS10_000840"
     CDS             32202..32564
                     /locus_tag="OQS10_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS2291950.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="OQS10_000841"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="OQS10_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="OQS10_000842"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="OQS10_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="OQS10_000843"
     CDS             35034..35912
                     /locus_tag="OQS10_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS2291951.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="OQS10_000844"
     CDS             35923..36276
                     /locus_tag="OQS10_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS2291952.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="OQS10_000845"
     CDS             36288..37592
                     /locus_tag="OQS10_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS2291953.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="OQS10_000846"
     CDS             37604..38689
                     /locus_tag="OQS10_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS2291954.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OQS10_000847"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OQS10_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS2291955.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OQS10_000848"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OQS10_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS2291956.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OQS10_000849"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OQS10_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OQS10_000850"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OQS10_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS2291957.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43714)
                     /gene="frlA"
                     /locus_tag="OQS10_000851"
     CDS             complement(42378..>43714)
                     /gene="frlA"
                     /locus_tag="OQS10_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS2291958.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11126 c        11989 g        10442 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaactcg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccca
//
LOCUS       DAJVXP010000015        43477 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVXP010000015 DAJVXP010000000
VERSION     DAJVXP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.015"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..77)
                     /locus_tag="OQS10_000852"
     tRNA            complement(1..77)
                     /locus_tag="OQS10_000852"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(81..173)
                     /locus_tag="OQS10_000853"
     tRNA            complement(81..173)
                     /locus_tag="OQS10_000853"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Ser,seq:gct)
     gene            complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQS10_000854"
     CDS             complement(489..674)
                     /gene="csrA"
                     /locus_tag="OQS10_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS2291959.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQS10_000855"
     CDS             complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OQS10_000855"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS2291960.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQS10_000856"
     CDS             complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OQS10_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS2291961.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQS10_000857"
     CDS             complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OQS10_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS2291962.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQS10_000858"
     CDS             complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OQS10_000858"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS2291963.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQS10_000859"
     CDS             complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OQS10_000859"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS2291964.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7519..8082
                     /gene="srlA"
                     /locus_tag="OQS10_000860"
                     /gene_synonym="gutA"
     CDS             7519..8082
                     /gene="srlA"
                     /locus_tag="OQS10_000860"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS2291965.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8079..9038
                     /gene="srlE"
                     /locus_tag="OQS10_000861"
                     /gene_synonym="gutE"
     CDS             8079..9038
                     /gene="srlE"
                     /locus_tag="OQS10_000861"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS2291966.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9049..9420
                     /gene="srlB"
                     /locus_tag="OQS10_000862"
                     /gene_synonym="gutB"
     CDS             9049..9420
                     /gene="srlB"
                     /locus_tag="OQS10_000862"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS2291967.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9424..10192
                     /gene="srlD"
                     /locus_tag="OQS10_000863"
                     /pseudo
     CDS             9424..10192
                     /gene="srlD"
                     /locus_tag="OQS10_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10298..10657
                     /gene="gutM"
                     /locus_tag="OQS10_000864"
     CDS             10298..10657
                     /gene="gutM"
                     /locus_tag="OQS10_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS2291968.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10724..11497
                     /gene="srlR"
                     /locus_tag="OQS10_000865"
                     /gene_synonym="gutR"
     CDS             10724..11497
                     /gene="srlR"
                     /locus_tag="OQS10_000865"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS2291969.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11490..12455
                     /gene="gutQ"
                     /locus_tag="OQS10_000866"
     CDS             11490..12455
                     /gene="gutQ"
                     /locus_tag="OQS10_000866"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS2291970.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQS10_000867"
     CDS             complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OQS10_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS2291971.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14153..15592
                     /gene="norV"
                     /locus_tag="OQS10_000868"
     CDS             14153..15592
                     /gene="norV"
                     /locus_tag="OQS10_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS2291972.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15589..16722
                     /gene="norW"
                     /locus_tag="OQS10_000869"
     CDS             15589..16722
                     /gene="norW"
                     /locus_tag="OQS10_000869"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS2291973.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQS10_000870"
     CDS             complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OQS10_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS2291974.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQS10_000871"
     CDS             complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OQS10_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS2291975.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQS10_000872"
     CDS             complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OQS10_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS2291976.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21200..22657
                     /gene="ascF"
                     /locus_tag="OQS10_000873"
     CDS             21200..22657
                     /gene="ascF"
                     /locus_tag="OQS10_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS2291977.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22666..24090
                     /gene="ascB"
                     /locus_tag="OQS10_000874"
     CDS             22666..24090
                     /gene="ascB"
                     /locus_tag="OQS10_000874"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS2291978.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24204..24623)
                     /locus_tag="OQS10_000875"
     CDS             complement(24204..24623)
                     /locus_tag="OQS10_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2291979.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24620..24931)
                     /locus_tag="OQS10_000876"
     CDS             complement(24620..24931)
                     /locus_tag="OQS10_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCS2291980.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25093..25866)
                     /locus_tag="OQS10_000877"
     CDS             complement(25093..25866)
                     /locus_tag="OQS10_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291981.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQS10_000878"
     CDS             complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OQS10_000878"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS2291982.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQS10_000879"
     CDS             complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OQS10_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS2291983.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQS10_000880"
     CDS             complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OQS10_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS2291984.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNRGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQS10_000881"
     CDS             complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OQS10_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS2291985.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQS10_000882"
     CDS             complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OQS10_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS2291986.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQS10_000883"
     CDS             complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OQS10_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS2291987.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQS10_000884"
     CDS             complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OQS10_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS2291988.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKFGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQS10_000885"
     CDS             complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OQS10_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS2291989.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQS10_000886"
     CDS             complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OQS10_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS2291990.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33953..34303
                     /gene="hypA"
                     /locus_tag="OQS10_000887"
     CDS             33953..34303
                     /gene="hypA"
                     /locus_tag="OQS10_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS2291991.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34307..35179
                     /gene="hypB"
                     /locus_tag="OQS10_000888"
     CDS             34307..35179
                     /gene="hypB"
                     /locus_tag="OQS10_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS2291992.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35170..35442
                     /gene="hypC"
                     /locus_tag="OQS10_000889"
     CDS             35170..35442
                     /gene="hypC"
                     /locus_tag="OQS10_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS2291993.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35442..36563
                     /gene="hypD"
                     /locus_tag="OQS10_000890"
     CDS             35442..36563
                     /gene="hypD"
                     /locus_tag="OQS10_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS2291994.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36560..37570
                     /gene="hypE"
                     /locus_tag="OQS10_000891"
     CDS             36560..37570
                     /gene="hypE"
                     /locus_tag="OQS10_000891"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS2291995.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37644..39721
                     /gene="flhA"
                     /locus_tag="OQS10_000892"
                     /pseudo
     CDS             37644..39721
                     /gene="flhA"
                     /locus_tag="OQS10_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39758..40111)
                     /locus_tag="OQS10_000893"
     CDS             complement(39758..40111)
                     /locus_tag="OQS10_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS2291996.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40188..40322)
                     /locus_tag="OQS10_000894"
     CDS             complement(40188..40322)
                     /locus_tag="OQS10_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2291997.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40398..42959
                     /gene="mutS"
                     /locus_tag="OQS10_000895"
     CDS             40398..42959
                     /gene="mutS"
                     /locus_tag="OQS10_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS2291998.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43065..43477
                     /locus_tag="OQS10_000896"
                     /pseudo
     CDS             43065..43477
                     /locus_tag="OQS10_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10293 a        11935 c        11326 g         9923 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga tgcagctttt
      121 gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc accacacgcc
      181 tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt
      241 actttctgag acttataagt caaacaattt ttcccacact tttatcgttt gcacacttca
      301 cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa aacggaggtc
      361 tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg
      421 aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa agatgagacg
      481 cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg gtagatctct
      541 tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg gttgcccttt
      601 acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc aactcgacga
      661 gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc
      721 ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta catgtaagca
      781 gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact tcaacctgac
      841 tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg cattgcctga
      901 cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa cgctgcaggt
      961 aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc acccttgccg
     1021 cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc tgtgacgtcc
     1081 ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag cacgataatt
     1141 gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt cggctcaaca
     1201 ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt actggaaaga
     1261 tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt ttctaactga
     1321 cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc gcctttcagc
     1381 agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat agcgccttct
     1441 ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga gatgatgcgg
     1501 aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc ggtggagaaa
     1561 tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag cgccatcgca
     1621 cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa attgcgacga
     1681 atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat cgcttcgttg
     1741 tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg cgatacatga
     1801 gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg attcagacga
     1861 atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac tttcagagaa
     1921 cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt atcttccacc
     1981 gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc gccggattcc
     2041 gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt gatggcatca
     2101 accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag atggtcatag
     2161 cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc accaaagccg
     2221 ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc agcttcgtca
     2281 actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa gccataggtg
     2341 tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt tgccagctct
     2401 tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc ttcagtcttc
     2461 agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga gcccataacg
     2521 tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag catattgccg
     2581 tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc attggacggc
     2641 attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc
     2701 gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat caacgtgcgg
     2761 aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat acgctccaga
     2821 cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc tgcctggcgg
     2881 ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga
     2941 cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg
     3001 ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg cgcacctttg
     3061 ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc ccagatttcg
     3121 taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg cagggcaaac
     3181 catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt gaaatagtcg
     3241 ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt gtaaccgaca
     3301 ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt ggtagcgcgg
     3361 gaataattac gcttgtcgag tccaaggaac acatccttga actggttcat cccggcgttg
     3421 gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac tacctgatgt
     3481 cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct cttgctcata
     3541 attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg
     3601 ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt aaaataccgt
     3661 atgcgttcag tcggcaaaat ttagccaaat atcctggata tcttccatca gatagccacg
     3721 atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca aaggttcacc
     3781 atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac attcacgcat
     3841 cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac gaatacgtgc
     3901 aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc ggctgtcatc
     3961 gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg ccgtagcatc
     4021 aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc gcagttcttg
     4081 ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc gagcatatgc
     4141 cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa agcaaaaggg
     4201 ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga gtgtgcagcc
     4261 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc tgttcccctc
     4321 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4381 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4441 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4501 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     4561 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     4621 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     4681 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     4741 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     4801 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     4861 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     4921 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     4981 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5041 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5101 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5161 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5221 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5281 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5341 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5401 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5461 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     5521 tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     5581 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
     5641 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     5701 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     5761 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     5821 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     5881 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     5941 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6001 ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc aaaaatttat
     6061 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6121 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6181 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6241 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6301 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6361 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6421 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6481 tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     6541 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     6601 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     6661 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     6721 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     6781 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     6841 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     6901 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     6961 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7021 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt cgatgaactg
     7081 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgaggctc
     7141 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7201 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7261 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
     7321 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
     7381 gctatccttt tattcaaact ttcaaattaa aatatttatc tttcattttg cggtcaaaat
     7441 aacactttca aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
     7501 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
     7561 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
     7621 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
     7681 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
     7741 ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat gccggaaaag
     7801 tacaaaccca gctactacgc ggcagcctct tatagctgcc actcaatgaa tggcctcttt
     7861 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
     7921 ctgaacctgc cacttggccc actggcggtg agctatctgc tggttggtct ggtcaccaac
     7981 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
     8041 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
     8101 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
     8161 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     8221 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     8281 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     8341 ttaatatcca ctcgacgggc aagtccagtc cgctggcgca gtacattgtg gaagatattt
     8401 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     8461 cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc gatggtttac
     8521 tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg
     8581 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc tcggcgctca
     8641 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
     8701 cgagccatcc actaggtctg gtcatgctgg cgctcatctg ctccttcccg ctgctttcac
     8761 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     8821 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     8881 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     8941 ttcgcgtcgg tgtcccttct gtactggtga gccacttttt aaccggcgca ccgactgtac
     9001 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     9061 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     9121 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     9181 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
     9241 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     9301 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     9361 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     9421 aaaatgaatc aggttgtcgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     9481 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     9541 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta tagttttggt
     9601 gctgacgcca ccagcgagca aagcgtcctg gcgctctctc gtggggtaga tgaaatcttt
     9661 gggcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac
     9721 ttccagctcg gcgattttga ccgtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
     9781 ttctcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
     9841 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
     9901 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
     9961 gctcggtaac ctgctgaaat cgcctatgtt ccagtcactg ttaccccaat acgcgaccaa
    10021 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10081 cggctgcgat tatcaggatg tgctgaatat gctgctgctc tatgccagtc ctaaggcgtc
    10141 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttgt aatcaacagc
    10201 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    10261 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    10321 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    10381 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    10441 ctttaaacca cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgtcgacac
    10501 cttgtttatg aaaggactga ccgtcttcgc acgaccgcaa aaaattcccg caattaccgg
    10561 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    10621 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    10681 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    10741 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    10801 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    10861 aggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    10921 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    10981 tagttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    11041 ggttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagtc tgcatatcgt
    11101 caatgcgcta tctgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11161 taaaaaatcg gcctcatttc acggacagct ggcagagaat gccttcgaac atttcagttt
    11221 cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacctttaa
    11281 cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    11341 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    11401 taagctgatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    11461 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    11521 agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    11581 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    11641 gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacatcgg
    11701 ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg
    11761 atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc
    11821 gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    11881 gcctggcggc aaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    11941 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    12001 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccca gccggggcac
    12061 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12121 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12181 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12241 tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga
    12301 caggcggcac cacgttacag gcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    12361 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaatta
    12421 acctgcagga tttctatcag gccgggatta tttaatcctt cattcccaga cgtttcgcca
    12481 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttatgat
    12541 gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    12601 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa
    12661 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    12721 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    12781 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagccccagc cgcaaacgac
    12841 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    12901 gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca
    12961 tcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13021 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13081 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag agtgccgtta tccgccattt
    13141 caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    13201 actcactttc cgccacactt tccggcagtg cagcacagtt gagatagacc agcggattca
    13261 ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    13321 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    13381 gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg
    13441 catcgcctgg catcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    13501 ctcccgccgc cagcgcagca atcagccgta gctcttcgtc gctgaaaaca tcgaactgat
    13561 cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca
    13621 gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat
    13681 cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca
    13741 gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga
    13801 tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca
    13861 gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    13921 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    13981 caatttgact atagatattg tcatatcgac catttgattg atagtcattt tgactactca
    14041 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgtaat
    14101 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    14161 tgtggtgaaa aataacattc attgggttgg tcaacgtgac tgggaagtgc gtgattttca
    14221 cggtacggaa tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga
    14281 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    14341 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    14401 ccacgctggg gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac
    14461 tgccaacgct atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt
    14521 ggtgaaaact ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    14581 gccaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    14641 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    14701 tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag
    14761 ccgcctggta acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    14821 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    14881 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    14941 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    15001 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    15061 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    15121 cgccggtctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    15181 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    15241 gggtttcgaa atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga
    15301 gttatgccgt gaacatggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    15361 gagcacggtg aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg
    15421 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    15481 gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga
    15541 atgctctctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    15601 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    15661 gacgccagta ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg
    15721 gacctcagcc atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg
    15781 ggtgaatttg ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc
    15841 gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    15901 ctggcaactg gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    15961 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    16021 ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    16081 aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa
    16141 gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    16201 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt
    16261 agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca
    16321 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    16381 aatgacgata tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc
    16441 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    16501 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    16561 gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg
    16621 gtggctcgcg gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg
    16681 atgaaagagg catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa
    16741 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccag
    16801 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    16861 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    16921 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    16981 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    17041 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    17101 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg
    17161 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg
    17221 aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    17281 cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg
    17341 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    17401 ctgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    17461 gccacggctg cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    17521 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct
    17581 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact
    17641 gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca
    17701 gattcatttc acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
    17761 attgcggggt aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    17821 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    17881 ccaggcagaa ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa
    17941 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    18001 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    18061 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    18121 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    18181 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    18241 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    18301 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    18361 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    18421 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    18481 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    18541 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    18601 cacggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    18661 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    18721 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    18781 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cggatggtga
    18841 actcagtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    18901 gcggcgggca gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga
    18961 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    19021 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    19081 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    19141 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    19201 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    19261 catcagcgcc gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac
    19321 acaaatcagc gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    19381 gcacaggtca catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct
    19441 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    19501 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    19561 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    19621 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    19681 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacagca
    19741 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    19801 cagttaatgc gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga
    19861 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    19921 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    19981 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    20041 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    20101 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    20161 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    20221 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    20281 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    20341 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    20401 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    20461 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    20521 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    20581 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    20641 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    20701 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt
    20761 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    20821 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    20881 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    20941 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    21001 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    21061 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    21121 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    21181 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    21241 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt
    21301 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    21361 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    21421 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    21481 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    21541 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    21601 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    21661 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    21721 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    21781 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    21841 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    21901 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    21961 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    22021 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    22081 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    22141 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    22201 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    22261 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    22321 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    22381 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    22441 agcgccaggg ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    22501 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    22561 cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt
    22621 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    22681 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    22741 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    22801 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    22861 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    22921 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    22981 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    23041 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    23101 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt
    23161 gaagcatttg atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg
    23221 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    23281 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    23341 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    23401 agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt
    23461 atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa
    23521 ggggtaacca tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    23581 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc
    23641 agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    23701 tggggtattg atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag
    23761 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    23821 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    23881 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc
    23941 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    24001 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    24061 attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct
    24121 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    24181 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    24241 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    24301 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    24361 agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca
    24421 aactcttggt tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga
    24481 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    24541 tctgagtcac tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    24601 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat
    24661 actcggcgtg attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg
    24721 caatcaccct taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa
    24781 aattgtcgag gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct
    24841 taataacccg atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact
    24901 cttcaaatgg cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt
    24961 aaacagccac atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc
    25021 aagttcacaa tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca
    25081 ctctgcaaaa agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg
    25141 ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag
    25201 tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca
    25261 gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc
    25321 ggaatcaaac tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc
    25381 atctacgcca gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc
    25441 ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact
    25501 tggtaaccag ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg
    25561 ccctttgccc gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg
    25621 atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc
    25681 agcaacgccc acttttctaa aatgccaggt ttgctcggca tcataaggca tggtcaggag
    25741 aggtttcagc gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa
    25801 gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat
    25861 attcatttcg ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa
    25921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    25981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    26041 ttcgccaatg tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt
    26101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    26161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    26221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    26281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    26341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    26401 gaatatcatg cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg
    26461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    26521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    26581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    26641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    26701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    26761 tcatcggata cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg
    26821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    26881 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    26941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc
    27001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    27061 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg
    27121 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac cacctctgtt
    27181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    27241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    27301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    27361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga
    27421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    27481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    27541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    27601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    27661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg
    27721 cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt
    27781 tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    27841 aagatgcagc gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca
    27901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc
    27961 gggttctgct ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    28021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    28081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    28141 actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    28201 gggtccaagc taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag
    28261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    28321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    28381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta
    28441 tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga
    28501 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg
    28561 acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca
    28621 acgttactga aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt
    28681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    28741 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    28801 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag
    28861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg
    28921 agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    28981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg
    29041 gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    29101 taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag
    29161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    29221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    29281 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    29341 ttacgcagcg gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc
    29401 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    29461 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    29521 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    29581 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    29641 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    29701 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    29761 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    29821 ctaaaaatgc aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac
    29881 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    29941 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    30001 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    30061 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    30121 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    30181 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    30241 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    30301 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    30361 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    30421 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    30481 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    30541 aggcatccgg gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca
    30601 acacacccgg cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    30661 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    30721 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    30781 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    30841 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    30901 agcggtaatc accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga
    30961 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    31021 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    31081 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    31141 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    31201 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    31261 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    31321 ggcaacaaac gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc
    31381 ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    31441 ggagataacc ggcattttct tgccaatacc accgaatttt tcgatatcgc gatgaccggt
    31501 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    31561 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    31621 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    31681 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    31741 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    31801 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    31861 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    31921 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    31981 ttgcatacgc atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca
    32041 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    32101 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    32161 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    32221 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    32281 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    32341 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    32401 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    32461 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    32521 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    32581 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    32641 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    32701 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    32761 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    32821 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    32881 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    32941 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    33001 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    33061 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    33121 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    33181 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    33241 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    33301 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    33361 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    33421 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    33481 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    33541 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    33601 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    33661 gtagatgtgc cactggtcct gtaggcgaca atgccgctgt gcgatgtaat cggctttctc
    33721 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    33781 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    33841 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    33901 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    33961 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    34021 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    34081 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    34141 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    34201 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    34261 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    34321 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    34381 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    34441 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    34501 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    34561 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    34621 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    34681 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    34741 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    34801 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    34861 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    34921 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    34981 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    35041 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    35101 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    35161 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    35221 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    35281 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    35341 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    35401 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    35461 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    35521 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    35581 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    35641 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    35701 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    35761 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    35821 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    35881 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    35941 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    36001 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    36061 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    36121 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    36181 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    36241 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    36301 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    36361 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    36421 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    36481 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    36541 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    36601 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    36661 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    36721 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    36781 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    36841 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    36901 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    36961 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    37021 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    37081 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    37141 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    37201 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    37261 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    37321 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    37381 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    37441 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    37501 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    37561 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    37621 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    37681 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    37741 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    37801 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata
    37861 tgaagacgaa actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac
    37921 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct
    37981 ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
    38041 tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct
    38101 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa
    38161 caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat
    38221 taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
    38281 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
    38341 caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag
    38401 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
    38461 gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta
    38521 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
    38581 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct
    38641 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
    38701 ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa
    38761 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
    38821 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
    38881 gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat
    38941 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
    39001 tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga
    39061 taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
    39121 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
    39181 ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga
    39241 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
    39301 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
    39361 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
    39421 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
    39481 cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
    39541 tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct
    39601 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
    39661 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
    39721 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac
    39781 gatatttttc cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta
    39841 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    39901 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc
    39961 cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg
    40021 gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    40081 gtttttcacg agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta
    40141 tattacaact taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac
    40201 gcaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc
    40261 ggaaccagca tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc
    40321 atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg
    40381 gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc
    40441 agcagtatct caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg
    40501 atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac
    40561 tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg
    40621 cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac
    40681 aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta
    40741 cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg
    40801 ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt
    40861 ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc
    40921 cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg
    40981 gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc
    41041 tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt
    41101 gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata
    41161 ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc
    41221 gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc
    41281 tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag
    41341 tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca
    41401 ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc
    41461 tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac
    41521 tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg
    41581 agaagatggg cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac
    41641 cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg
    41701 agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc
    41761 gtgaacgtac cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca
    41821 ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc
    41881 tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca
    41941 cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc
    42001 tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc
    42061 tggtgaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata
    42121 aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc
    42181 catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc
    42241 cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct
    42301 acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta
    42361 cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga
    42421 ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga
    42481 tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc
    42541 tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc
    42601 taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag cacggcgaca
    42661 ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac ggtctggcgg
    42721 ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa aaactgcgtg
    42781 agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg cagatgtctt
    42841 tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg
    42901 attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat
    42961 aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa
    43021 aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct
    43081 atcaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg
    43141 aatatcagtt attacaatcc cgttaacatc aactctcttt ttaccctgaa accgacttac
    43201 ttatttccac aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc
    43261 taaaccaacc ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat
    43321 tcgaaactgg cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa
    43381 atgattcaga gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata
    43441 ttattgaaat cattaatgac aacataaaat atgccat
//
LOCUS       DAJVXP010000016        42136 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVXP010000016 DAJVXP010000000
VERSION     DAJVXP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.016"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="OQS10_000897"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="OQS10_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS2291999.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="OQS10_000898"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="OQS10_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS2292000.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="OQS10_000899"
     CDS             complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="OQS10_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS2292001.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4218..4622
                     /locus_tag="OQS10_000900"
     CDS             4218..4622
                     /locus_tag="OQS10_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS2292002.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4648..5361
                     /gene="msyB"
                     /locus_tag="OQS10_000901"
     CDS             4648..5361
                     /gene="msyB"
                     /locus_tag="OQS10_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS2292003.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5510..6076
                     /gene="satP"
                     /locus_tag="OQS10_000902"
     CDS             5510..6076
                     /gene="satP"
                     /locus_tag="OQS10_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS2292004.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6111..6698)
                     /gene="mog"
                     /locus_tag="OQS10_000903"
     CDS             complement(6111..6698)
                     /gene="mog"
                     /locus_tag="OQS10_000903"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS2292005.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6813..7766)
                     /gene="tal"
                     /locus_tag="OQS10_000904"
     CDS             complement(6813..7766)
                     /gene="tal"
                     /locus_tag="OQS10_000904"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS2292006.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8045..9474
                     /locus_tag="OQS10_000905"
                     /pseudo
     CDS             8045..9474
                     /locus_tag="OQS10_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9544..10320
                     /gene="yaaA"
                     /locus_tag="OQS10_000906"
     CDS             9544..10320
                     /gene="yaaA"
                     /locus_tag="OQS10_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS2292007.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10473..10769)
                     /locus_tag="OQS10_000907"
     CDS             complement(10473..10769)
                     /locus_tag="OQS10_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS2292008.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="OQS10_000908"
     CDS             complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="OQS10_000908"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS2292009.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="OQS10_000909"
     CDS             complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="OQS10_000909"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS2292010.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="OQS10_000910"
     CDS             complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="OQS10_000910"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS2292011.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15693..15812)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="OQS10_000911"
     CDS             complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="OQS10_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS2292012.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16027..16713)
                     /locus_tag="OQS10_000912"
     CDS             complement(16027..16713)
                     /locus_tag="OQS10_000912"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS2292013.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17113..17253)
                     /locus_tag="OQS10_000913"
     CDS             complement(17113..17253)
                     /locus_tag="OQS10_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292014.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17349..18065
                     /gene="arcA"
                     /locus_tag="OQS10_000914"
     CDS             17349..18065
                     /gene="arcA"
                     /locus_tag="OQS10_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS2292015.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18125..19477)
                     /gene="creD"
                     /locus_tag="OQS10_000915"
     CDS             complement(18125..19477)
                     /gene="creD"
                     /locus_tag="OQS10_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS2292016.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19535..20959)
                     /gene="creC"
                     /locus_tag="OQS10_000916"
     CDS             complement(19535..20959)
                     /gene="creC"
                     /locus_tag="OQS10_000916"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS2292017.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20959..21648)
                     /gene="creB"
                     /locus_tag="OQS10_000917"
     CDS             complement(20959..21648)
                     /gene="creB"
                     /locus_tag="OQS10_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS2292018.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21661..22134)
                     /gene="creA"
                     /locus_tag="OQS10_000918"
     CDS             complement(21661..22134)
                     /gene="creA"
                     /locus_tag="OQS10_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS2292019.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22345..23214
                     /gene="robA"
                     /locus_tag="OQS10_000919"
     CDS             22345..23214
                     /gene="robA"
                     /locus_tag="OQS10_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS2292020.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="OQS10_000920"
     CDS             complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="OQS10_000920"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS2292021.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23910..24431
                     /gene="yjjX"
                     /locus_tag="OQS10_000921"
     CDS             23910..24431
                     /gene="yjjX"
                     /locus_tag="OQS10_000921"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS2292022.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="OQS10_000922"
     CDS             complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="OQS10_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS2292023.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="OQS10_000923"
     CDS             complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="OQS10_000923"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS2292024.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27080..28747
                     /gene="ettA"
                     /locus_tag="OQS10_000924"
     CDS             27080..28747
                     /gene="ettA"
                     /locus_tag="OQS10_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS2292025.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="OQS10_000925"
     CDS             complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="OQS10_000925"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS2292026.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30307..31689)
                     /gene="radA"
                     /locus_tag="OQS10_000926"
     CDS             complement(30307..31689)
                     /gene="radA"
                     /locus_tag="OQS10_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS2292027.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31738..32706)
                     /gene="serB"
                     /locus_tag="OQS10_000927"
     CDS             complement(31738..32706)
                     /gene="serB"
                     /locus_tag="OQS10_000927"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS2292028.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32812..33456
                     /locus_tag="OQS10_000928"
     CDS             32812..33456
                     /locus_tag="OQS10_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS2292029.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33484..34500
                     /gene="lplA"
                     /locus_tag="OQS10_000929"
     CDS             33484..34500
                     /gene="lplA"
                     /locus_tag="OQS10_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS2292030.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34532..34681
                     /locus_tag="OQS10_000930"
     CDS             34532..34681
                     /locus_tag="OQS10_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292031.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34956..35675)
                     /gene="deoD"
                     /locus_tag="OQS10_000931"
     CDS             complement(34956..35675)
                     /gene="deoD"
                     /locus_tag="OQS10_000931"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS2292032.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35755..36978)
                     /gene="deoB"
                     /locus_tag="OQS10_000932"
     CDS             complement(35755..36978)
                     /gene="deoB"
                     /locus_tag="OQS10_000932"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS2292033.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37030..38352)
                     /gene="deoA"
                     /locus_tag="OQS10_000933"
     CDS             complement(37030..38352)
                     /gene="deoA"
                     /locus_tag="OQS10_000933"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS2292034.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38479..39258)
                     /gene="deoC"
                     /locus_tag="OQS10_000934"
     CDS             complement(38479..39258)
                     /gene="deoC"
                     /locus_tag="OQS10_000934"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS2292035.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39516..41066
                     /locus_tag="OQS10_000935"
     CDS             39516..41066
                     /locus_tag="OQS10_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS2292036.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41038..41901
                     /locus_tag="OQS10_000936"
     CDS             41038..41901
                     /locus_tag="OQS10_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS2292037.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9952 a        11409 c        10586 g        10189 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     2161 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat
     2221 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     2281 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     2341 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt
     2401 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     2461 catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt tcaccctgca
     2521 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     2581 tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     2641 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     2701 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     2761 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     2821 ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt
     2881 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     2941 tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa
     3001 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     3061 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     3121 ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg
     3181 tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     3241 cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca
     3301 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     3361 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     3421 tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt
     3481 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     3541 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     3601 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     3661 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     3721 caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc
     3781 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     3841 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     3901 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     3961 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     4021 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     4081 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     4141 gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc
     4201 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     4261 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     4321 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     4381 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     4441 ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
     4501 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
     4561 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4621 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4681 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     4741 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4801 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
     4861 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     4921 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
     4981 gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag
     5041 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     5101 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
     5161 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     5221 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5281 ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct gcgccggatg
     5341 gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa
     5401 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta
     5461 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5521 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5581 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     5641 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5701 cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg cgattctgct
     5761 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5821 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5881 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     5941 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     6001 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     6061 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     6121 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     6181 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     6241 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     6301 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     6361 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     6421 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6481 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     6541 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6601 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6661 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6721 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     6781 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6841 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6901 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6961 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     7021 agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     7081 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     7141 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     7201 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     7261 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7321 tctttttcca gctgttctgc agcacggata ccctgccaag tagaagccag tttgatcaga
     7381 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7441 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7501 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     7561 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7621 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7681 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7741 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7801 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7861 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7921 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7981 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     8041 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     8101 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     8161 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     8221 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     8281 cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc
     8341 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     8401 tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggggat
     8461 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8521 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt tcccccgctg
     8581 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     8641 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     8701 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8761 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8821 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     8881 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg gattgtccag
     8941 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     9001 ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     9061 atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt tattctgttt
     9121 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9181 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9241 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9301 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
     9361 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9421 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9481 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9541 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9601 acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca tgaagcgcgg
     9661 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
     9721 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggct
     9781 attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac
     9841 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
     9901 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9961 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    10021 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    10081 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    10141 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10201 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10261 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10321 gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10381 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10441 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10501 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
    10561 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10621 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    10681 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    10741 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10801 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    10861 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    10921 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10981 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    11041 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    11101 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    11161 tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc gtgcggctcc
    11221 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11281 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11341 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11401 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11461 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11521 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11581 actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    11641 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    11701 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    11761 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    11821 ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11881 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11941 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    12001 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    12061 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    12121 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
    12181 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    12241 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12301 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12361 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    12421 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12481 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    12541 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    12601 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12661 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12721 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12781 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12841 gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    12901 caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt catcgccact
    12961 ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac gatattttcc
    13021 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    13081 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    13141 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13201 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccagcagc
    13261 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13321 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    13381 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    13441 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13501 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    13561 ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga gaatcaatag
    13621 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    13681 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13741 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    13801 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13861 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    13921 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13981 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    14041 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    14101 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    14161 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    14221 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14281 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14341 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    14401 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14461 taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta agccttcttt
    14521 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
    14581 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14641 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14701 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    14761 gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    14821 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14881 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    14941 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    15001 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    15061 aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca gcaccatgtg
    15121 atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    15181 ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg taacgttgtg
    15241 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15301 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15361 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15421 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    15481 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15541 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15601 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15661 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
    15721 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
    15781 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
    15841 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
    15901 taatttactc atggcaggta accagttcag aagctgctat cagacactct ttttttaatc
    15961 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
    16021 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
    16081 gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc
    16141 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
    16201 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
    16261 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
    16321 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
    16381 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
    16441 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
    16501 gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa taacatcacc
    16561 agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg
    16621 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
    16681 ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga ccaaataacc
    16741 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
    16801 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
    16861 aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
    16921 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
    16981 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
    17041 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
    17101 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
    17161 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
    17221 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
    17281 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
    17341 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
    17401 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
    17461 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
    17521 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
    17581 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
    17641 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
    17701 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
    17761 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
    17821 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
    17881 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
    17941 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
    18001 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
    18061 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
    18121 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
    18181 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
    18241 ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg agcagtcccc
    18301 acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt
    18361 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
    18421 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
    18481 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
    18541 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
    18601 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt
    18661 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
    18721 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
    18781 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
    18841 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
    18901 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
    18961 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
    19021 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
    19081 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
    19141 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
    19201 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
    19261 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acaagttttt
    19321 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
    19381 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
    19441 gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt gcaatggcag
    19501 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
    19561 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
    19621 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
    19681 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
    19741 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
    19801 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
    19861 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
    19921 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
    19981 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
    20041 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
    20101 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
    20161 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
    20221 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
    20281 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
    20341 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
    20401 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    20461 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    20521 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
    20581 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    20641 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
    20701 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgagcca
    20761 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    20821 ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    20881 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    20941 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    21001 aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    21061 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
    21121 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    21181 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    21241 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    21301 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    21361 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    21421 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa
    21481 accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    21541 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    21601 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    21661 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    21721 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    21781 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    21841 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
    21901 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
    21961 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    22021 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    22081 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    22141 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    22201 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    22261 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    22321 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    22381 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    22441 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    22501 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    22561 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    22621 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    22681 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    22741 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    22801 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    22861 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    22921 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
    22981 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    23041 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
    23101 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
    23161 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
    23221 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
    23281 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    23341 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    23401 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    23461 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    23521 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    23581 atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    23641 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    23701 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    23761 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    23821 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    23881 taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg accaccaatc
    23941 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    24001 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
    24061 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    24121 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    24181 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
    24241 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
    24301 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    24361 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
    24421 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    24481 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    24541 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    24601 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
    24661 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    24721 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    24781 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    24841 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    24901 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    24961 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
    25021 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    25081 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    25141 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    25201 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    25261 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    25321 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    25381 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
    25441 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
    25501 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
    25561 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    25621 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    25681 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
    25741 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    25801 gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    25861 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
    25921 gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct
    25981 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    26041 gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt gcattttcag
    26101 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
    26161 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    26221 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    26281 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
    26341 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    26401 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    26461 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    26521 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    26581 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    26641 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    26701 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    26761 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    26821 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    26881 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    26941 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    27001 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    27061 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    27121 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    27181 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    27241 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    27301 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    27361 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    27421 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    27481 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    27541 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    27601 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    27661 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    27721 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    27781 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    27841 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    27901 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    27961 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    28021 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    28081 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    28141 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    28201 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt
    28261 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    28321 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    28381 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    28441 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    28501 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    28561 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    28621 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    28681 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    28741 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    28801 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    28861 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    28921 tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    28981 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    29041 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    29101 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    29161 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    29221 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    29281 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    29341 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    29401 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    29461 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    29521 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    29581 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    29641 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    29701 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    29761 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    29821 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    29881 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    29941 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    30001 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    30061 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    30121 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    30181 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    30241 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    30301 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    30361 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    30421 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    30481 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    30541 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    30601 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    30661 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    30721 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    30781 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    30841 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    30901 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    30961 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    31021 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    31081 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    31141 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    31201 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    31261 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    31321 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    31381 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    31441 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    31501 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    31561 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    31621 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    31681 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    31741 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    31801 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    31861 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    31921 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    31981 gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    32041 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    32101 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    32161 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    32221 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    32281 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    32341 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    32401 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    32461 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    32521 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    32581 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    32641 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    32701 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    32761 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    32821 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    32881 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    32941 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    33001 atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    33061 gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcccgttca
    33121 ggcgaaagcg tcgaagtgcg cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat
    33181 tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc
    33241 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    33301 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    33361 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    33421 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    33481 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    33541 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    33601 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    33661 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    33721 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    33781 atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
    33841 ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc
    33901 tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa tgccgacctt
    33961 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    34021 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    34081 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    34141 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    34201 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    34261 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    34321 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    34381 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    34441 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    34501 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    34561 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    34621 taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    34681 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag aaatttatgg
    34741 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    34801 ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat aatagtaaat
    34861 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc ccccactccg
    34921 gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    34981 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    35041 tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt cgcgccaaat
    35101 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    35161 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    35221 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    35281 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    35341 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    35401 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    35461 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttgtaa
    35521 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    35581 gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    35641 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc aacatgtttt
    35701 caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    35761 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    35821 atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc cgtaaaccag
    35881 taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac aaccgtggtc
    35941 agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg gcagacggcg
    36001 gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc cccaggaaga
    36061 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    36121 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    36181 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    36241 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    36301 acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac gaccgatgtt
    36361 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccag
    36421 gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg tatagaaaat
    36481 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    36541 gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat ccagcagctc
    36601 ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa ccgggacacc
    36661 ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    36721 gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc
    36781 ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg gacctttacg
    36841 accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg tgtcagcccc
    36901 gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    36961 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa tgatcagatc
    37021 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    37081 ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt
    37141 cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac gcgccatatc
    37201 agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc cgcccattgc
    37261 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    37321 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    37381 ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    37441 cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat
    37501 aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac gatactcacc
    37561 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    37621 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    37681 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    37741 tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca gaatagaggc
    37801 ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt
    37861 atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt ctttaataat
    37921 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    37981 gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat agccgccgca
    38041 ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac cagtggaatg
    38101 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    38161 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    38221 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc cgttgataaa
    38281 gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa taatttcttg
    38341 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    38401 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    38461 aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt ggcccagcgc
    38521 tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg catctgccca
    38581 gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    38641 cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac ggatcacttc
    38701 catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg tagaggtttt
    38761 gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    38821 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    38881 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcgcggta
    38941 cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg tttctgccag
    39001 cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga ttcggatttc
    39061 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    39121 gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa ttactttctc
    39181 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    39241 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    39301 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    39361 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    39421 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    39481 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    39541 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    39601 attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    39661 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    39721 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa tggctggagc
    39781 tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt taccaccacg
    39841 taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg ttgcaaccat
    39901 atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc tggcgttacc
    39961 tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct gattcgccca
    40021 ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg aacctgacct
    40081 ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct
    40141 gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag
    40201 ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc
    40261 tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta
    40321 cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat
    40381 tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta
    40441 accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc
    40501 tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca aatgaagtgg
    40561 gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct
    40621 ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc ggcaccacgc
    40681 cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg caaactgtcg
    40741 ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc gacgaaacca
    40801 tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc
    40861 gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc
    40921 gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg
    40981 gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg
    41041 aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc
    41101 agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg
    41161 tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg
    41221 ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca gtgtgatacc
    41281 tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag cgtggacgaa
    41341 gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt aagcggcggc
    41401 gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg
    41461 caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga aaccggctgg
    41521 gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg ggggagcgaa
    41581 tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat ctgtttgctt
    41641 gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca ggtggattat
    41701 ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt tccggttcgc
    41761 ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg
    41821 ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata
    41881 tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag
    41941 gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc ggatgcggcg
    42001 tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcag
    42061 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    42121 cggttcggca cagact
//
LOCUS       DAJVXP010000017        40909 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVXP010000017 DAJVXP010000000
VERSION     DAJVXP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.017"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..789
                     /locus_tag="OQS10_000937"
     CDS             <1..789
                     /locus_tag="OQS10_000937"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCS2292038.1"
                     /translation="LITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNL
                     LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTY
                     PLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMN
                     PISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDL
                     TNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            791..1027
                     /locus_tag="OQS10_000938"
     CDS             791..1027
                     /locus_tag="OQS10_000938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292039.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1200..1875)
                     /locus_tag="OQS10_000939"
                     /pseudo
     CDS             complement(1200..1875)
                     /locus_tag="OQS10_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2020..2628)
                     /locus_tag="OQS10_000940"
     CDS             complement(2020..2628)
                     /locus_tag="OQS10_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS2292040.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2812..3129)
                     /gene="metJ"
                     /locus_tag="OQS10_000941"
     CDS             complement(2812..3129)
                     /gene="metJ"
                     /locus_tag="OQS10_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS2292041.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3406..4566
                     /gene="metB"
                     /locus_tag="OQS10_000942"
     CDS             3406..4566
                     /gene="metB"
                     /locus_tag="OQS10_000942"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS2292042.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4569..7001
                     /gene="metL"
                     /locus_tag="OQS10_000943"
     CDS             4569..7001
                     /gene="metL"
                     /locus_tag="OQS10_000943"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS2292043.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7350..8240
                     /gene="metF"
                     /locus_tag="OQS10_000944"
     CDS             7350..8240
                     /gene="metF"
                     /locus_tag="OQS10_000944"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS2292044.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8569..10749
                     /gene="katG"
                     /locus_tag="OQS10_000945"
     CDS             8569..10749
                     /gene="katG"
                     /locus_tag="OQS10_000945"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS2292045.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10843..11748
                     /gene="yijE"
                     /locus_tag="OQS10_000946"
     CDS             10843..11748
                     /gene="yijE"
                     /locus_tag="OQS10_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS2292046.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11775..12392)
                     /locus_tag="OQS10_000947"
     CDS             complement(11775..12392)
                     /locus_tag="OQS10_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS2292047.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12666..13769)
                     /gene="gldA"
                     /locus_tag="OQS10_000948"
     CDS             complement(12666..13769)
                     /gene="gldA"
                     /locus_tag="OQS10_000948"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS2292048.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13780..14442)
                     /gene="fsa"
                     /locus_tag="OQS10_000949"
     CDS             complement(13780..14442)
                     /gene="fsa"
                     /locus_tag="OQS10_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS2292049.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14454..16955)
                     /gene="ptsP"
                     /locus_tag="OQS10_000950"
     CDS             complement(14454..16955)
                     /gene="ptsP"
                     /locus_tag="OQS10_000950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS2292050.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17264..18343
                     /locus_tag="OQS10_000951"
     CDS             17264..18343
                     /locus_tag="OQS10_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS2292051.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18358..18678
                     /locus_tag="OQS10_000952"
                     /pseudo
     CDS             18358..18678
                     /locus_tag="OQS10_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18729..21025
                     /locus_tag="OQS10_000953"
                     /pseudo
     CDS             18729..21025
                     /locus_tag="OQS10_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20991..21869
                     /locus_tag="OQS10_000954"
     CDS             20991..21869
                     /locus_tag="OQS10_000954"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS2292052.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21871..22212
                     /locus_tag="OQS10_000955"
     CDS             21871..22212
                     /locus_tag="OQS10_000955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS2292053.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22199..23050)
                     /locus_tag="OQS10_000956"
     CDS             complement(22199..23050)
                     /locus_tag="OQS10_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2292054.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFESIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23265..24998)
                     /locus_tag="OQS10_000957"
     CDS             complement(23265..24998)
                     /locus_tag="OQS10_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS2292055.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25181..27832)
                     /gene="ppc"
                     /locus_tag="OQS10_000958"
     CDS             complement(25181..27832)
                     /gene="ppc"
                     /locus_tag="OQS10_000958"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS2292056.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28184..29335)
                     /gene="argE"
                     /locus_tag="OQS10_000959"
     CDS             complement(28184..29335)
                     /gene="argE"
                     /locus_tag="OQS10_000959"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS2292057.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29489..30493
                     /gene="argC"
                     /locus_tag="OQS10_000960"
     CDS             29489..30493
                     /gene="argC"
                     /locus_tag="OQS10_000960"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS2292058.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30501..31277
                     /gene="argB"
                     /locus_tag="OQS10_000961"
     CDS             30501..31277
                     /gene="argB"
                     /locus_tag="OQS10_000961"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS2292059.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31338..32711
                     /gene="argH"
                     /locus_tag="OQS10_000962"
     CDS             31338..32711
                     /gene="argH"
                     /locus_tag="OQS10_000962"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS2292060.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32978..33895
                     /gene="oxyR"
                     /locus_tag="OQS10_000963"
     CDS             32978..33895
                     /gene="oxyR"
                     /locus_tag="OQS10_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS2292061.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33878..35278)
                     /gene="sthA"
                     /locus_tag="OQS10_000964"
     CDS             complement(33878..35278)
                     /gene="sthA"
                     /locus_tag="OQS10_000964"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS2292062.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35599..36249
                     /gene="fabR"
                     /locus_tag="OQS10_000965"
     CDS             35599..36249
                     /gene="fabR"
                     /locus_tag="OQS10_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS2292063.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36249..36608
                     /locus_tag="OQS10_000966"
     CDS             36249..36608
                     /locus_tag="OQS10_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS2292064.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36648..37748)
                     /gene="trmA"
                     /locus_tag="OQS10_000967"
     CDS             complement(36648..37748)
                     /gene="trmA"
                     /locus_tag="OQS10_000967"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS2292065.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37862..38052
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38117..39961
                     /gene="btuB"
                     /locus_tag="OQS10_000968"
     CDS             38117..39961
                     /gene="btuB"
                     /locus_tag="OQS10_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS2292066.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39906..40763
                     /gene="murI"
                     /locus_tag="OQS10_000969"
     CDS             39906..40763
                     /gene="murI"
                     /locus_tag="OQS10_000969"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS2292067.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9449 a        10196 c        11237 g        10027 t
ORIGIN      
        1 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
       61 agccagttaa cgtcagccac cgggcctgac gggctggaaa tacgccggga atatgatgaa
      121 tggggcaacc tgctgaatga agagaacccg catcagctgc agcagcttat tcgtttgccg
      181 gggcagcagt atgatgagga gtccggcctg tattataacc gccaccgcta ttatgacccg
      241 ctgcaggggc ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat
      301 acatatccgc tgagcccggt gaatagcatg gatccattag gattatatga atttaaatca
      361 aaaaatattg atgatattgg aatatttgca ttggcaatgt gtaatggaga atcaattaac
      421 gagaataaag aatatggtgg actaatatgt aagaagcaag gtgaatattt ccccatgaat
      481 ccgataagtt caaatgataa tgatagtgta gacttgcgaa atataaaatg ccctgaaggt
      541 tcagagagag taggcgatta tcacactcac ggtttttact ctgacgataa aggaaataaa
      601 gtaacaaaag aaaatgatgt ttatgatagt ctaaattttt caagcaaaga tttaacgaat
      661 tcttatatga atggaatggg aaaaaaagaa tacagtagtt acttgggaac accaaataac
      721 acctatctaa aatataatcc caaagctaaa gggaatggag ttacaattat caggcaaggg
      781 agtaattaaa atgaaggcaa tacaatgttg ttatttcctt gctttagtct ccagtcattt
      841 aatggctgga gttatgggtg atgataatat atttttaagt aattcggatt cagctgcttt
      901 atattgtgtg gctaaaatat ttccagatgg agaaaatatt ttaatatata aagtaaatat
      961 aattaatgct agttcaaaaa aagatgtttt ttttatgaaa gcccaacatc aacccacaat
     1021 gacataatgg ggggcggggg aatgtcatat gcctatgaat atgatgtaaa atataatata
     1081 tgtcatttga gaaaaaagag cagaatgagg taaaaatctt tggctgtgta ttcagtctgg
     1141 taatcatcca aggaaagctt atcagaaggg tgatgactcc aattagtaga acatatattt
     1201 tcgataaaag caccgatgac tttttcatga agttctacag aacgcgagaa acagatggtt
     1261 ctgcgggcca gacgtttgat tcgggttctc agggtcagat tgttacgctc aatgtgctga
     1321 gtaaaaatct taccagtcag atgcttctcc ttcggtaact ctctagcgta actaccccat
     1381 tcgtcagtgg tcaacatgcc gatggtaaaa gggtaagcag cgcccgcaat tcacgacagg
     1441 tttcgtcagt ccgggggcca aaagtgtagg ccagaacacc accagttttc gtgttgtaca
     1501 cgtaccagag ccagtgctgt cgcgctttac tgccgacaaa gctccattgc tgactaagtt
     1561 tgcagatgag tgccacatca gcatgagcga ccggggatga cgttattctt cgtggcgaga
     1621 gttttttaaa gttcggatga ctgtattaat gccgattttc agcgtacggg cagtatcgcg
     1681 aaccccggca ccgttgaaag tcatttcagc gatttggtct ttaactcccg gtttacgggg
     1741 ctcgtaacag taactgagct gaaatacccg gtgacagtca cggccgcgga atcgctcatg
     1801 tctttttggg ttctgcccgt gacggtaaac ctgaacggac tggcaacggg ggcaatgaag
     1861 gttaactgtg accatgaaac aaccttaaaa gcgagtatta tatccttaat tcaactaatt
     1921 ggaggcatca ccgtttaccg gagcgacagc ttgcggaaag gtcgtgcaac tacaaagcaa
     1981 caaacagact acggcgggaa ttattttttt catcatccat taccacgaaa acagcggcca
     2041 ctctttggcg actgtggtga gttgtggcgc gtcgaaaacg gcttgtggtg aaactaccgt
     2101 ttcctcaagc ggaatatttt tgccgtagcg ttctttgagc ttagcctgga ccagcgggtt
     2161 gctgagatcg aactctttca acttctgctg ctcgcccacg cgcattccca gcgcgttctg
     2221 gtaaaccttc cacaccactt ccgaacagta ctggcgatcg tcgctccatg agaagctgaa
     2281 atcatacggt ttaccgagat aacgttttgc cgtttgcgcc agtttttgct gttgttcaac
     2341 actcagtccg ccttcaacgc ggcgaacaac gtatttgccc ttttcaccat gggcgatcca
     2401 ctgcttgagc ggggggtatt tcaccgggcc gaccgcttca aaaacgtagg gctttttgtt
     2461 gcgtatcacc agcataccgg tgtggctata atcggaatgg gtcgccagtt ggatcgcttt
     2521 actttgcgat gagcgagaga tctgaaagat gatgtcgccg gtttgtggct gccaggcaaa
     2581 ggcaggtaca gaaaccagca ggctgaggat cagcagcctg tttttcatag ttaaacgtcc
     2641 atgtataaaa agcggtgggt cgcagacaac gtgctcgttg tttatgccgg atgcggcgtg
     2701 aacgccttat ccggcctaca agttcgtgca aattcaataa attgcaatat gacgtaggcc
     2761 tgataagcgt agcgcatcag gcgattccac tccgcgccgc tcttttttgc tttagtattc
     2821 ccacgtctcc gggttaatcc ccatctcacg catgatctct tttgccgctt ccgggatttc
     2881 gtcgctgcgc tctttacgca gatcggcatc atccggcaaa ggttgcccgg taaaggcatg
     2941 cagaaacgct tcgcacagca gctcgctgtt ggtagcgtga cgcaggttgt tcacctgacg
     3001 acgcgtgcgt tcatcggtga ggatttttaa caccttaaga ggaatggaaa ccgtaatctt
     3061 tttgacttgt tcactcttct tgccgtgctc agcgtatggg ctgatatatt cgccgctcca
     3121 ttcagccatg agatacttaa tcctcttcgt caataaattg agaccagacc acacaattta
     3181 tgtgggtact gaccgtaaac ccgcatagtt taccgtacag gcgttaccgt gacatcgtgt
     3241 aatgcacctg tcggcgtgat aatgcatata attttaacgg ctatttggga tttgctcaat
     3301 ctatacgcaa agaagtttag atgtccagat gtattgacgt ccattaacac aatgtttact
     3361 ctggtgcctg acatttcacc gacaaagccc aggaaacttc atcacatgac gcgtaaacag
     3421 gccaccatcg cagtgcgtag cgggttaaat gacgacgaac agtatggttg cgttgtccct
     3481 cccatccatc tttccagcac ctataacttt accggattta atgaaccgcg cgcgcatgat
     3541 tactcgcgtc gcggcaaccc aacgcgcgat gtggttcagc gtgcgctggc agaactggaa
     3601 ggcggtgctg gcgcagtact tactaatacc ggcatgtccg cgattcacct ggtaacgacc
     3661 gtctttttga aacctggcga tctgctggtt gcgccgcacg actgctacgg cggtagctat
     3721 cgcctgttcg acagtctggc gaaacgcggt tgctatcgcg tgttgtttgt tgatcaaggc
     3781 gatgaacagg cattacgggc agcgctggca gaaaaaccca aactggtact ggtagaaagc
     3841 ccaagtaatc cattgttacg cgtcgtggat attgcgaaaa tctgccatct ggcaagggaa
     3901 gtcggggcgg tgagcgtggt ggataacacc ttcttaagcc cggcattaca aaatccgctg
     3961 gcattaggtg ccgatctggt gttgcattca tgcacgaaat atctgaacgg tcactctgac
     4021 gtggtggctg gcgtggtgat tgctaaagac ccggacgttg tcactgaact tgcctggtgg
     4081 gcaaacaata ttggcgtgac gggcggcgcg tttgacagct atctgctgct acgcgggttg
     4141 cgaacgctgg tgccgcgtat ggagctggcg cagcgcaacg cgcaggcgat tgtgaaatat
     4201 ctgcaaaccc agccgttggt gaaaaaactg tatcacccgt cgctgccgga aaatcagggg
     4261 catgaaattg ccgcgcgtca gcaaaaaggc tttggcgcaa tgttgagttt tgaactggat
     4321 ggcgatgagc agacgctgcg tcgtttcctg agcgggctgt cgttgtttac gctggcggaa
     4381 tcattagggg gagtggaaag tttaatctct cacgccgcaa ccatgacaca tgcaggcatg
     4441 gcaccagaag cgcgtgctgc cgccgggatc tccgagacgc tgctgcgtat ctccaccggt
     4501 attgaagatg gcgaagattt aattgccgac ctggaaaatg gcttccgggc tgcaaacaag
     4561 gggtaaaaat gagtgtgatt gcgcaggcag gggcgaaagg tcgtcagctg cataaatttg
     4621 gtggcagtag tctggctgat gtgaagtgtt atttgcgtgt cgcgggcatt atggcggagt
     4681 actctcagcc tgacgatatg atggtggttt ccgccgccgg tagcaccact aaccagttga
     4741 ttaactggtt gaaactaagc cagaccgatc gtctctctgc gcatcaggtt caacaaacgc
     4801 tgcgtcgcta tcagtgcgat ctgattagcg gtctgctacc cgctgaagaa gccgatagcc
     4861 tcattagcgc ttttgtcagc gatctggaac gcctggcggc gctgctcgac agcggtatta
     4921 acgacgcagt gtatgcggaa gtggtgggcc acggggaagt atggtcggca cgcctgatgt
     4981 ctgcggtact taatcaacaa gggctgccag cggcctggct tgatgcccgc gagtttttac
     5041 gcgctgaacg cgccgcacaa ccgcaggttg atgaagggct ttcttacccg ttgctgcaac
     5101 agctactggt gcaacatccg ggcaaacgtc tggtggtgac cgggtttatc agccgtaaca
     5161 acgccggtga aacggtgctg ctggggcgta acggttccga ctattccgcg acacaaatcg
     5221 gtgcgctggc gggtgtttct cgtgtaacca tctggagcga cgtcgccggg gtatacagtg
     5281 ccgacccgcg taaagtgaaa gatgcctgtc tgctgccgtt gctgcgtctg gatgaagcca
     5341 gcgaactggc tcgtctggcg gctcccgttc ttcacgcccg tactttacag ccggtttctg
     5401 gcagcgaaat cgacctgcaa ctgcgctgta gctacacgcc ggatcaaggt tccacgcgca
     5461 ttgaacgcgt gctggcctcc ggtactggtg cgcgtattgt caccagccac gatgatgtct
     5521 gtttgattga gtttcaggtg cccgccagtc aggatttcaa actggcgcat aaagagatcg
     5581 accaaatcct gaaacgcgcg caggtacgcc cgctggcggt tggcgtacat aacgatcgcc
     5641 agttgctgca attttgctac acctcagaag tggccgacag tgcgctgaaa atcctcgacg
     5701 aagcggggtt acctggcgaa ctgcgcctgc gtcaggggct ggcgctggtg gcgatggtcg
     5761 gtgcaggcgt cacccgtaac ccgctgcatt gccaccgctt ctggcagcaa ctgaaaggcc
     5821 agccggtcga atttacctgg cagtccgatg acggcatcag cctggtggca gtactgcgca
     5881 ccggcccgac cgaaagcctg attcaggggc tgcatcagtc cgtcttccgc gcagaaaaac
     5941 gcatcggcct ggtattgttc ggtaagggca atatcggttc ccgttggctg gaactgttcg
     6001 cccgtgagca gagcacgctt tcggcacgta ccgactttga atttgtgctg gcaggcgtgg
     6061 tggacagccg ccgcagtctg ttgagctatg acgggctgga cgccagccgc gcgttagcct
     6121 tcttcaacga tgaagcggtt gagcaggatg aagagtcgtt gttcctgtgg atgcgcgccc
     6181 atccgtatga tgatttagtg gtgctggacg ttaccgccag ccagcagctt gctgatcagt
     6241 atcttgattt cgccagccac ggtttccacg tcatcagcgc caacaaactg gcgggagcca
     6301 gcgacagcaa taaatatcgc cagatccacg acgccttcga aaaaaccggg cgtcactggc
     6361 tgtacaatgc caccgtcggt gcgggcttac cgatcaacca caccgtgcgc gatctgatcg
     6421 acagcggcga tactattttg tcgatcagcg ggatcttctc cggcacgctc tcctggttgt
     6481 tcctgcaatt cgacggtagc gtgccgttta ccgagctggt ggatcaggcg tggcagcagg
     6541 gcttaaccga acctgacccg cgtgacgatc tctctggcaa agacgtgatg cgcaagctgg
     6601 tgattctggc gcgtgaagca ggttacaaca tcgaaccgga tcaggtacgc gtggagtcgc
     6661 tggtgcctgc ccattgcgaa ggcggcagca tcgaccattt ctttgaaaat ggcgatgaac
     6721 ttaacgagca gatggtgcaa cggctggaag cggcccgcga aatggggctg gtgctgcgct
     6781 acgtggcgcg tttcgatgcc aatggcaaag cgcgtgtggg cgtggaagcg gtgcgtgaag
     6841 accatccgct ggcatcactg ctgccgtgcg ataacgtctt tgccatcgaa agccgctggt
     6901 atcgcgataa tccgctggtg atccgtgggc ctggcgctgg gcgcgacgtc accgccgggg
     6961 cgattcagtc ggatatcaac cggctggcac agttgttgta atttcagaaa tttaataatg
     7021 cccggtactc atgttttcgg gtttatggtt tctaatgaaa tatattgaat tatcatagga
     7081 ttaggccgga ttaagcgttt acgacgaatc cggcaagaag caataagtac atggttagtt
     7141 tatatttgca gtccggtttg ctttgcatac cggattttct ttttcttacc atcctgaagt
     7201 tttttcatct tccctgattt ttcctcacca tcattggtca tttttcggtt gacgcccttc
     7261 ggcttttcct tcatctttac atctggacgt ctaaacggat agatgtgcac aacacaacat
     7321 ataactacaa gcgattgatg aggtaaggta tgagcttttt tcacgccagc cagcgggatg
     7381 ccctgaatca gagcctggca gaagtccagg ggcagattaa cgtttcgttc gagtttttcc
     7441 cgccgcgtac cagtgaaatg gagcagaccc tgtggaactc catcgatcgc cttagcagcc
     7501 tgaaaccgaa gtttgtatcg gtgacctatg gcgcgaactc cggcgagcgc gaccgtacgc
     7561 acagcattat taaaggcatt aaagatcgca ctggtctgga agcggcaccg catcttactt
     7621 gcattgatgc gacgcccgac gagttgcgca ccattgcacg cgactactgg aataacggta
     7681 ttcgtcatat cgtggcgctg cgtggcgatc tgccgccggg aagtggtaag ccagaaatgt
     7741 atgcttctga cctggtgacg ctattaaaag aagtggcaga tttcgatatc tccgtggcgg
     7801 cgtatccgga agttcacccg gaagcaaaaa gcgctcaggc ggatttgctt aatctgaaac
     7861 gcaaagtcga tgccggagcc aaccgcgcga ttactcagtt cttcttcgat gtcgaaagct
     7921 acctgcgttt tcgtgaccgc tgtgtatcgg cgggcattga tgtggaaatt attccgggaa
     7981 ttttgccggt atctaacttt aaacaggcga agaaatttgc cgatatgacc aacgtgcgta
     8041 ttccggcgtg gatggcgcaa atgttcgacg gcctggatga tgatgccgaa acccgcaaac
     8101 tggtgggcgc gaatattgcc atggatatgg tgaagatttt aagccgtgaa ggagtgaaag
     8161 atttccactt ctatacgctt aaccgcgctg aaatgagtta cgcgatttgc catacgctgg
     8221 gtgttcgacc tggtttataa atagtgtggc ttttgtgaaa atcacacagt gatcacaaat
     8281 tttaaacaga gcacaaaatg ctgcctcgaa atgagggcgg gaaaataagg ttgtcagcct
     8341 tgttttctcc ctcattactt gaaggatatg aagctaaaac ccttttttat aaagcatttg
     8401 tccgaattcg gacataatca aaaaagctta attacgatca atttgaccta catctcttta
     8461 accaacaata tgtaagatct caactatcgc atccgtggat taattcaatt ataacttctc
     8521 tctaacgctg tgtatcgtaa cggtaacact gtagagggga gcacattgat gagcacgtca
     8581 gacgatatcc ataacacgac agccactggc aaatgcccgt tccatcaggg cggtcacgac
     8641 cagagcgcgg gggcgggcac aaccactcgc gactggtggc caaatcaact ccgtgttgac
     8701 ctgttaaacc aacattctaa tcgttctaac ccgctgggtg aggactttga ctaccgcaaa
     8761 gaattcagca aattagatta ctacggcctg aaaaaagatc tgaaagccct gctgacagaa
     8821 tctcagccgt ggtggccagc cgactggggc agctacgccg gtctgtttat tcgtatggcc
     8881 tggcacggcg caggtactta ccgttcaatc gatggacgcg gcggcgcggg tcgtggtcag
     8941 caacgttttg caccgctgaa ctcctggccg gataacgtaa gcctcgataa agcgcgtcgc
     9001 ctgttgtggc caatcaaaca gaaatatggt cagaaaatct cctgggccga cctgtttatc
     9061 ctcgcaggta acgtggcgct ggaaaactcc ggcttccgta ccttcggttt tggtgccggt
     9121 cgtgaagacg tctgggaacc ggatctggat gttaactggg gtgatgaaaa agcctggctg
     9181 actcaccgtc atccggaagc gctggcgaaa gcaccgctgg gtgcaaccga gatgggcctg
     9241 atttacgtta acccggaagg cccggatcac agcggcgaac cgctttctgc ggcagcagct
     9301 atccgcgcga ccttcggcaa catgggcatg aacgacgaag aaaccgtggc gctgattgcg
     9361 ggtggtcata cgctgggtaa aacccacggt gccggtccga catcaaacgt aggtcctgat
     9421 ccagaagctg caccgattga agaacaaggt ttaggctggg cgagcactta cggcagcggc
     9481 gttggcgcag atgccattac ctctggtctg gaagttgtct ggacccagac gccgacccag
     9541 tggagcaact atttcttcga aaacctgttc aaatatgagt gggtacagac ccgcagtccg
     9601 gctggcgcaa tccagttcga agcggtagac gcaccggaaa ttatcccgga tccattcgat
     9661 ccgtcgaaga aacgtaaacc gacgatgctg gtgaccgacc tgacgctgcg ttttgatcct
     9721 gagttcgaga agatctctcg tcgtttcctc aacgatccgc aggcgttcaa cgaagccttt
     9781 gcccgtgcct ggttcaaact gacgcacagg gatatggggc cgaaatctcg ctacatcggg
     9841 ccggaagtgc cgaaagaaga tctgatctgg caagatccgc tgccgcagcc gatctacaac
     9901 ccgaccgagc aggacattat cgatctgaaa ttcgcgattg cggattctgg tctgtctgtt
     9961 agtgagcggg tatcggtggc ctgggcatct gcttctacct tccgtggtgg cgacaaacgc
    10021 ggtggtgcca acggtgcgcg tctggcatta atgccgcagc gcgactggga tgtgaacgcc
    10081 gcagccgttc gtgctctgcc tgttctggag aaaatccaga aagagtctgg taaagcctcg
    10141 ctggcggata tcatcgtgct ggctggtgtg gttggtgttg agaaagccgc aagcgccgca
    10201 ggtttgagca ttcatgtacc gtttgcgccg ggccgcgttg atgcgcgtca ggatcagact
    10261 gacattgaga tgttcgagct gctggagcca attgctgacg gtttccgtaa ctatcgcgct
    10321 cgtctggatg tttccaccac cgagtcactg ctgatcgaca aagcccagca actgaccctg
    10381 accgcgccgg aaatgaccgc gctggtgggc gggatgcgcg tactgggtgc caacttcgat
    10441 ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat tgagcaatga cttcttcgtg
    10501 aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg aatcgaaaga gctgtttgaa
    10561 ggccgtgacc gcgaaaccgg cgaagtgaaa tacactgcca gccgtgcgga tctggtattt
    10621 ggttctaact ctgtcctgcg tgcggtggct gaagtttacg ccagcagcga tgctcacgag
    10681 aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga tgaacctcga ccgtttcgac
    10741 ctgctgtaat ctgaccttct tcagcgactg cctttcaggc agtcgctgaa gtttctttac
    10801 cggcgtatag tgtccacagg aaaactacac actggatctc tcatgtctgc cgcaggaaag
    10861 agcaacccac tggcaatcag tggcctggtt gtgctcacac ttatctggag ttatagctgg
    10921 attttcatga agcaagtcac cagctacatc ggtgccttcg actttaccgc cttacgctgc
    10981 attttcggcg ctctcgtttt attcatcgtc cttttattac gtggtcgcgg aatgcgcccg
    11041 acaccgttta aatacacctt agccattgcc ctgttacaaa cctgcgggat ggttggcctg
    11101 gcgcagtggg cgctggtcag cggcggagcg gggaaggtgg cgatcctaag ctataccatg
    11161 ccgttctggg tggtgatttt cgccgcgttg tttctcggtg aacgcctgcg acgtgggcaa
    11221 tatttcgcga tactgattgc cgctttcggc ttatttttgg tgttgcagcc gtggcaactc
    11281 gatttctctt cgatgaaaag tgccatgctg gcaatcctct ccggcgtcag ttggggggcg
    11341 agcgcgattg ttgctaaacg tctgtatgcc cgtcatccgc gcgtggattt attgtcgtta
    11401 acatcctggc agatgctcta tgcggcgctg gtgatgagtg tggtcgcttt actggtgccg
    11461 caacgtgaaa ttgactggca gcccaccgtg ttctgggcgc tggcctacag tgcgattctg
    11521 gcgacggcac tggcgtggag cttatggttg tttgtattga aaaacttgcc tgccagtatt
    11581 gccagcttaa gcacactggc cgttcccgtt tgcggcgtac tcttttcctg gtggctgctc
    11641 ggcgagaatc cgggggccgt tgaaggtagc ggtattgtgc tgattgtgat ggcactggcg
    11701 ctggtgagcc gtaagaaaaa agaagccgtt agtgtaaaaa ggatctgaat tttttcttca
    11761 tgtggggcga tcgcttattt aacaaaataa cgataatgcc ccaccatgcg ccagctaaac
    11821 agcacatctt cttcctgcgc acctgcgcca atgttatgta tcaccagcgg cgtaccgtcg
    11881 cgggcgaagc catctgaaac caccccaata tgtgccagcc cgttatctaa tcgccaggag
    11941 acaatatcgc ccgcttgata gtcactgggg ttcttgctga tggggcgtgt tttatcgtgg
    12001 tgggtaaacc aggtttccag attaggcacc cgacggtgat cgatgttgct gtccgggcgc
    12061 tttaactgcc atttttgcgg gtactcggca aaattcttcg ccatatcttc gtgcaccagt
    12121 ttctgcaaat cgaccttctg gctgcgcaat gcacggatca ccacatcgga acataccccg
    12181 cgtttttgcg ggacatcacc gccaggatag gtaagctgca tatacgccgg atcgtaaaat
    12241 agcgtgctgc cgatttgctg tctggcaccg tctgcgatgg caaggttggt attggcctgg
    12301 atttgtacca cggttggtgg aacggcggga gattttaagg agtggctggt aaatgccgtt
    12361 agcaggctga gcagcgccag tgaagctttc atctggttcg tccgtgaaaa taagagtcac
    12421 agattcgacc ttccgggata acgattgttc tgtagagaaa atgtccagaa gcggataaaa
    12481 aactcctgaa caacgtggga gtttcagatt actggcacag agtgccggat gcggcgtgaa
    12541 cgccttatct gtcctaaaaa aaaagaaaat tcaatatatt gcagagattg cgtaggcctg
    12601 ataagcgtag cgcatcaggc aattttgcgt tcaaactccc ggacaagccg ggagtttgga
    12661 gtaggttatt cccactcttg caggaaacgc tgaccgtact ggtcggctac cagcagagcg
    12721 gcgtaaacct gatctggcgt cgcgccgcca ggcatgttgt ggatggtttc acattctgca
    12781 catgccgctt cagccacaat tcgcattttc gccgggacat cttctttaat atccagttga
    12841 gcgagagtta ttggcaaacc taccgcatgg ctaagtgcag ctacggtttc gatttcctca
    12901 accggtgcgt tttccagaac cagctgcgtc agcgtaccaa atgccacttt ttcaccgtga
    12961 taatagtgat gcgcgtccgg gatagcggtc aggccgttat gcactgcgtg cgccgcagcc
    13021 agaccaccac tttcaaaacc aacaccgctc aaataggtgt tcgcttcaat cacgcgctcc
    13081 agcgccggag tcactacatg ctgttcggca gcaagcatcg ctttttcgcc ttcttccagc
    13141 agggtgttgt agcacagttc agccagtgcc agcgcagcct gggtgcactt gccgcccgcc
    13201 atggtggtcg cgccgctacg agagcaggca cgcgcttcaa accaggttgc cagcgcatcg
    13261 ccgatacccg ccgctaacag acgtgcaggt gcgccagcga cgattttggt gtcgacaatg
    13321 accatattcg ggttatttgg caacagcaga tagcggtcaa actcaccctc atcggtgtag
    13381 ataacagaca atgcgctgca cggtgcatcg gtagaggcga tagtcggtgc gatcgctacc
    13441 ggaacaccca tgaaatgtgc cagtgctttg gctgtatcga gggtttttcc gccaccgata
    13501 ccgagaattg cgccacactg cgcagtctcc gcgatgccac gcagacggtc gatctcattt
    13561 tgcgaacatc caccgccaaa cggcgcaatt tctactacca gtccagcatc tttaaagctt
    13621 ttctcgacag tggattgagc aaaacctaaa acaaatttgt cacccaccac taaccagcgt
    13681 tctgccagcg gcttcaggta ttcgcccaga cgattaatca catcagcgcc ctggatgtat
    13741 ttacccggtg attgaataat gcggtccata attgctcctt tagagatgag tagtgccaaa
    13801 tgcggcattc cagtcgtgtt cgaacttctc tatagctgac tctaccgcag gggtgttgag
    13861 catttgttgc gctacatcta agggcagggt gatggattca catcctgcca gtaaacagtc
    13921 cagcgcctga cgcggcgttt taaagctggc tgccagcacc atgctttctg gcgcgtgcat
    13981 ttctaacagc gcttgcagct cctgaaccgt acgaatgccg tctccgccct gggcatctac
    14041 gcggttaaca tacggagcaa cgtattttgc ccctgccagc gcggccagta acccttgtgc
    14101 agcactgtac actgcggtgc caagcgtagt aataccctct tttttcagta ttttaattgc
    14161 tgccagacct tcggaagtca ccgggatttt caccacaata cccggaatag cgtcgcgcag
    14221 gcgcttcgct tcttccacca tcccctgcgc gtcgcggctc atggtctgag caaacagaat
    14281 gccctcatca ccaatcgctt tttgcagacg cggcagcact tcccatatgg actccttgct
    14341 ggcagcgata atgctcgggt tagttgtcac cccggcaatg gggaatatgc gtgccagacg
    14401 ttcgacttct gcgacgttag cggtgtccag atacagttcc atgatgtttc cttttacagt
    14461 tccagttcat gttgcagcag gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg
    14521 ttacggaatt cttcgtgcat gatgcggcga gcgaggcgcg agaaaatgcg catgtgctga
    14581 tcgcccgcag cgtgtttgtt cagggttaac atgatgatga attgcgcttc atcatcaccc
    14641 cagcgcaccg gcgcttgcag acgcgcaacg ctgatggtgg attgctcaat gtgttctgat
    14701 ttgctgtgtg gaatggcaaa actaaagccc agaccggtag agaaaacggc ctcgcgcgcc
    14761 cacaagtcgg cttccagttt acgcggatag cggcagcgcc ctgccagcag caggttatcg
    14821 gtcatgcctt tgagcacttc ttctttgctg cgccagtcgc tttccagtgt gatgcactcg
    14881 gcggtgacca gcggtgcgtc ttgttgggtc atgcggaatt gcgccagcag gtgctccact
    14941 tccagcgaag tacggcaggc cattgcctgg ttgagcaact tgcggcactc acggctatca
    15001 agttgcgcca tccgcgcttt cgccgccgga attgatggtg cgctcatgct gagttcatcc
    15061 agccctaagc cgaccagcaa cggcagcacg gaacctttcg ctcccagctc accgcacaga
    15121 ccaatccatt taccctggcg atgcaccgcc tgcacggcgt aatcgagcgc ccgcaagaat
    15181 gccggattca ggctgttgta gtgacgagta accttagcgt tatcgcgatc cactgccagc
    15241 agatattgcg tcaggtcatt actaccaata ctaaagaaat caatctcttc gcagcattga
    15301 tcgatgatga acatcaccga cggcacttcc agcataatgc cgagctggat cttctcatca
    15361 aacggaatgt gttcgttacg tagttgctgt ttggcttctg ccagtttttc tttcacccat
    15421 aagatctctt ccattgagga gatcatcggg atcatgattt tcaggctgcc gtgagcggag
    15481 gcgcgcagga tcgagcgtag ttgtgtggta aataacgacg cgtattcttc ataaatacgc
    15541 acggcgcgat aaccgaggaa cgggtttgcc tctgcgggaa tgtttagata atcaacgggt
    15601 ttgtcgccgc caatgtccat agtgcgcaca ataatgctgc gtccgttggc agactccagc
    15661 gcctgacaaa aaatgttgta cagctcgctt tcgcccggtg cactggtgcg atccatatag
    15721 agcatttcag tgcggaacaa accaacgcct tccgcaccat tgccgaatgc ggcctgcgct
    15781 tccacggagt gagcgatgtt agcggcaatt tcaatgcgga taccgtcagc ggtacgggct
    15841 tgttgggtca gccagacacg ctgttgctca cgcagggcgt cctgtacgcg ggcttcttgc
    15901 tgataataac gagctacggc ttctcctggc tcaaccacaa tcgccccggc gttgccgtcg
    15961 atataaatcg tttgatgctg ccacggagtg agggcatcaa tatccacacc aaccagcgtc
    16021 ggaatgttga acgaacgggc aaggatcacc gtatgtgagg tggtgccgcc acttttcagc
    16081 aacagacctt tgaggtgatt tttatccagt tcgaggaact ggctgggggt cagttcatca
    16141 gccatacaaa tggcggactg cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag
    16201 atttgctgga gtaactggaa gcagacgtcg cgtacgtcca gggcacgttc ttgcaggtag
    16261 ctgctgctgg aacgggcgaa ctcttcgcaa aagtgattcg cactggcgac aatcgcttcg
    16321 gcgcagctta atccctcgct gacgcctgcc agtaagtgct gacgtaaaga tgtatcgcca
    16381 gccagggatc ggtgagcttc cagtatcgcg ctggtagcac cgtcgctatc cagcagacga
    16441 aactcaatgt ttttcagtac cagcgtcagg ccgttttcca gtgtggattg ctcggcgtca
    16501 acgtctttgg ctgcgggaag attactcagc gcattgagat ctaaagaaga gatcggcgtc
    16561 agaatgccgc ctgcactacc gctgcacacg gtgcgggcgc ggataatttg cggattcaga
    16621 ttggtcagtg aaaccggcag tggttccagt tcgtcagatt taacttccgc cagcggcgca
    16681 tcgcagtggg ggaattcatc gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg
    16741 tcggccccgg aaatcagtaa ctggcagtta tcgcccgcca gcgtatcggt gccaatcagc
    16801 gcaagggcgc ttttggcgtt gcccttgcgg tcagtgtgca ggttatgcca ctcaatttgt
    16861 gatgaaaaag tattacacag cgtttcaacg tggcttgccg gacgcgcatg tacgccgtta
    16921 ggtagctcac aaataaattc cacaatcagg gccattgcct ctctcccggt acggttttct
    16981 gtttacagga taagagcgca cggcaacggc ctgccatgtg acaaatctgc caaaagctgg
    17041 acaaatgtaa tgtaaccgtc aatttgcgac gcgtctcaca agacgccgtt ttgcggcatg
    17101 cttccggttt atcgcaagtt atgaggcgga tcgcattttt gtactgatat tacaaaagtc
    17161 cagtaaatgg cctttttatc cactgtttgc tccgcctgcg attgcctatt gtttcctcca
    17221 tcaaaaatat ggacatgggc tttcgcaagc ccgggagcag cttatgaatg agttggtgca
    17281 gatcctgaaa aatacccgtc agcatttaat gacgggcgtt tcacacatga ttcccttcgt
    17341 ggtatcgggc ggtattttgc tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc
    17401 ggatgccgta gccgatccaa atctgaaaaa actgtttgat atcggcgttg cgggcttgac
    17461 gctgatggtg cctttcctcg ccgcttacat tggttattcc attgcagaac gttctgcgct
    17521 ggctccatgc gctatcggta cctgggttgg taacagcttt ggtgcgggct tctttggtgc
    17581 actgatcgcc gggattatcg gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca
    17641 taaagttctg cgctcggtga tgcctatctt cattattcct atcgtcggca cactgattac
    17701 cgcaggcatc atgatgtggg gcttgggcga gcctgtaggg gcgttgacca acagcctgac
    17761 tcagtggctt caggggatgc agcagggcag cattgttatg ctggcggtga tcatgggtct
    17821 gatgctggcg ttcgatatgg gcggtccggt taacaaagtg gcctatgcct tcatgctgat
    17881 ttgcgttgct cagggtgttt ataccgtggt ggccatcgct gccgtgggta tttgtatccc
    17941 gccgctgggg atggggctgg cgacgctgat tggtcgtaaa aatttctccg cagaagagcg
    18001 cgaaaccggc aaagcggcac tggtgatggg ctgtgttggc gttactggag gggcgattcc
    18061 tttcgccgct gccgatccgc tgcgtgtcat tccttccatc atggtcggtt cagtttgtgg
    18121 tgcagtaact gcggcgctgg tcggtgcgca gtgctatgca ggctggggtg gtctgattgt
    18181 gctgccggtg gttgaaggca agctgggtta tatcgcagca gtggctgtcg gagcagtggt
    18241 gacggctgtt tgtgtgaacg tgctgaaaag tctggcgcgt aaaaatgggt cttcgactga
    18301 tgaaaaagaa gacgacctgg atttggattt tgaaattaat taattgagga acgagccatg
    18361 acgaaaatta ttgcagtaac cgcatgtcct tcaggtgttg cccataccta tatggcagca
    18421 gaggcgctgg aaagtgccgc gaaagcgaaa ggctgggaag tgaaagtaga aacctagggg
    18481 tcaattggtc tggaaaatga actgactgcg gaagatgtgg cgagcgctga tatggtgatt
    18541 ctgaccaaag atatcggcat caagtttgaa gagcgttttg ctggcaagac catcgtgcgc
    18601 gtcaacatca gcgatgcagt aaaacgtgct gacgccatta tgagcaaaat tgaagctcac
    18661 ctggcgcaaa ccgcttaagt tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg
    18721 agttcctcat gacgaatcgt atctctcgcc tcaaaactgc actgtttgcc aatacccgtg
    18781 aaatctcgct ggagcgggcg ctgctttata ccgccagcca tcggcaaacc gaaggcgaac
    18841 cggtgatatt gcgccgggcg aaagcaacag cgtatatcct tgaacatgtt gaaatttcga
    18901 ttcgtgatga agaactgatt gccggtaacc gcaccgtaaa accgcgcgcc gggattatgt
    18961 cgccggaaat ggacccttac tggctgctga aagagctgga tcaattcccg acgcgtccgc
    19021 aggaccgctt tgctatcagc gaagaagata aacgtatcta ccgtgaagag ttgttcccgt
    19081 actgggaaaa acgttcgatg aaagatttca tcaacgggca gatgacagat gaagtaaaag
    19141 ccgcgaccaa cacgcagatt ttcagcatca accagacgga taaaggccag gggcacatta
    19201 ttattgatta cccacgcctg ttgaatcacg gtctgggtga gctggtggca cagatgcagc
    19261 aacattgtca gcaacagccg gagaatcact tttatcaggc cgcgttgtta ctgctggaag
    19321 cctcgcagaa acacattttg cgttacgccg aactggcgga aacgatggcg gcaaactgca
    19381 cagatgcaca gcgtcgcgaa gaactgctga ctattgcgga gatctctcgc cataacgccg
    19441 aacataagcc gcagacgttc tggcaggcgt gccagttatt ctggtacatg aacatcattc
    19501 tgcaatacga atccaacgcc agttcgctgt ctttagggcg cttcgaccag tatatgttgc
    19561 cgttctatca ggcatcttta acccagggcg aagatccggc gttcctgaaa gaactgctcg
    19621 aatctttatg ggtgaaatgc aacgacatcg tgctgttgcg ctccaccagc agcgcgcgtt
    19681 atttcgcagg tttcccgacc ggctataccg cactgctcgg cgggttaacc gagaacggac
    19741 gtagcgcggt gaacgtgctt tcgttccttt gccttgacgc ctatcaaagc gtgcaattac
    19801 cgcaaccgaa cctcggcgtg cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa
    19861 ccgccgaaac cattcgcctc ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg
    19921 taccagcgtt cctcaaccgt ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg
    19981 gctgtgtgga attatctatt cccggcagaa cctacggctt gcatgacatc gcgatgttta
    20041 atctgctgaa agtgatggaa atctgcctgc atgaaaatga aggcaatgct gcgctgactt
    20101 atgaaggttt actggagcag atccgcgcca agatcagcca ctacatcacc ctgatggttg
    20161 agggcagcaa tatttgtgat atcggccatc gcgactgggc acctgtaccg ctgctctcgt
    20221 cttttatcag cgattgtctg gaaaaaggtc gcgatattac cgatggcggc gcgcgttata
    20281 acttctccgg cgtacagggg atcggtatcg ccaacctgag cgattctctc catgcgttga
    20341 aagggatggt ttttgagcaa cagcgtttaa gttttgacga attgctgtcg gtattaaaag
    20401 ccaactttgc aacgccagaa ggcgaaaaag tccgtgctcg cttaattaac cgcttcgaga
    20461 aatacggtaa cgatatcgac gaggtggata acatcagcgc cgaactgtta cgccactact
    20521 gcaaagaagt ggaaaaatac cagaacccgc gcgcggttac ttcacgccgg gatcgtatac
    20581 cgtttctgct cacgtcccgt tgggatcggt ggttggcgcg acgccagacg gtcgttttgc
    20641 cggggaacaa ctggcagacg gcggcttgtc acctatgctg ggccaggacg cacaagggcc
    20701 aacagcggta ctgaagtcag tcagtaagct cgataatacg ctgctgtcta acggtacgtt
    20761 gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa gcgggattac gcaaactggc
    20821 cgacttctta cgggcgttta cccagcttaa gttgcagcat attcagttta acgtggtgaa
    20881 cgccgacacg ttgcgggaag cgcaacagcg cccacaagat tatgccgggc tggtggtgcg
    20941 cgttgccgga tacagcgcct tctttgtcga actgtcgaag gagatccagg atgacatcat
    21001 ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg ccgcgatgat gtggcgcgca
    21061 ttttcaacat tcagcgttat tcactgaatg acggcgaggg cattcgtacg gtggtctttt
    21121 ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc ggagtcgatc tccggcaaaa
    21181 tccagacggt acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg cgtgatgcgg
    21241 atgaatgccc ctccggggcg tttgaacgga ttggtcgcga tatcagcctt gacgctctgg
    21301 aacgggaagt gatgaaagat gacattttct ttcgcacgtc cggcggcggc gtcacgcttt
    21361 ctggcggcga agtgttaatg caggcggagt ttgctacccg ttttttacag cgactgcggc
    21421 tgtggggtgt gtcatgcgcc attgaaactg ccggagacgc gccagcaagc aagctgttac
    21481 cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa aattatggac gcgactcagg
    21541 cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga gaatctgcgt ttgctggtga
    21601 gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc tggtttcacg ctcagcaggg
    21661 agaatatgca gcaggcgctg gatgtactga tcccgctgaa aatcaggcag atccatctgt
    21721 taccgtttca tcagtacggc gaaccgaaat accgcctgct ggggaaaaca tggtcgatga
    21781 aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat gcgcgaaatg gcagaacggg
    21841 ccggatttca ggttaccgtg ggaggttaaa atggcatacc tggtggcagt aaccgcctgc
    21901 gtaagcggcg tggcgcatac ttatatggcg gcggaacggc tggaaaagtt gtgccagtta
    21961 gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg gaacggagaa tcgtttagcg
    22021 gacgaggata tccgtcgggc ggatgttgct ctgttgatta cggatatcga gcttgccggt
    22081 gccgagcgat ttgaacattg ccgctatgtg caatgcagca tctacgcatt cctgcgtgag
    22141 ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg cgccgcagca aacccatctt
    22201 attctggagt agtcggtttt tctgtcagtt ggctgtggta ctgccggcga tattccgacg
    22261 gcgagcgttc ggtgttttta cgaaacagac ggcagaagta gttgctgtcg acaaagccgc
    22321 aggcgtgcgc cacttctttt accttcaggt catagccttt caataacgtc ttagcgtgct
    22381 ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc ccccgttttt tgaaacaggt
    22441 gcgagagata gtttggtgaa atatagaatg cctgtgcgac agattcgcgg gtaagcgcgg
    22501 aggcatagcg ttcgtcgata taatcgcgaa tagattcaaa tagtgcctgg ctgcgtgagg
    22561 cggtctggat ttggctgccg agcaaatcgc ggcagtggct aagcaggctg gctacgataa
    22621 gccttgccgt ttgctgctcc tgcggctgca tttgcatttc attgagcgtt tgtagcagaa
    22681 aagaacctat acgtgggccg cgtcgggcga cgtgttgctt cgccagattt tgatattgtt
    22741 tgccatccca ttgcacgacg ctgaagccga gttgctgttt gccaaacagc acgctaaagg
    22801 tggtagcggg tgttttccat tgtgggaaat tccagccacc agcggcaacg tacagcacat
    22861 cgccgggaac taacgttgct ccagcgccgg tatcaacaaa ctcaccttcc agcacaattt
    22921 ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac cgggccgtta ctgctggcaa
    22981 agtaaatctg gcgcagggac agcgggccgt tgatcaggcg ggagagcaga tagctgacgc
    23041 cgtgatacat gttattccct taggtatgta gacgggaaaa cagtaactgg cattattaaa
    23101 aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg taaacattgc ggcagttgta
    23161 ttgccggatg tggcgtaaac gccttatcca gcctaccgcc gcgatctgta ggccggataa
    23221 gacgcgtcaa gcgtcgcatc cggcaaatag cgcctggctg ataattactg attacctacc
    23281 tggtcgccat acggcagagt gtcgtaatcg atcagcgcgt ttttcttgta cgggttacca
    23341 atccagcggg tagtttcttt gaactgcgga ttcaccactg aacgggttgg atcgtaaccg
    23401 tcgtaagata agcccgccaa atctgaccag gtgtggatca gttccgccag gctgtattta
    23461 cgatcgacat cctgtgagaa atcacgggga tgagtcgctt gccatttttc cgacgtccac
    23521 agcaggaacg gaatggtgta catgtggcgc gtcgggttgt cttcgttacg cccctgagtt
    23581 ttgtgcggcg gcgtgtcgta aacctcttca ccgtggtcag agaagtaaac caggaatccg
    23641 ttcgggtctg ccgctttaaa gtctttaatc aggctggcaa ccacatgatc gttatacagg
    23701 ttagcgttgt cataatcgtt atatgattcc agctcttctg cgcttaatcc tggcggaaca
    23761 tgatcggtat tgccatcaaa cttgccctga ttttccgggt agcggtattt gtatttgata
    23821 tgcgtaccca gcagatgaac gatgatcagt ttcttcggcg cagggtcatt cagcacatcc
    23881 tggaacggct tcagcacgtt ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc
    23941 atgtagtact gcttgtcggt ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc
    24001 atcgtctgct ggttggtgat ccagaaggtt ttataacccg cctgtttcat catgttcatc
    24061 agcgacggct gcgtcagata cagatccggg ttcttttcat tggcaaaggt cagcgcctgt
    24121 tgcaggattt caatggtgta cggacgagac gtaactacgt tattaaacac ggtcagattc
    24181 ggatcggttt tatgcagcgc atccagctcc ggcgtggttt cacgcggata accgtacaga
    24241 ctcatgcgtc cgcgctgggt cgactcgcca atcaccagca ctaaagtgcg cggttcgtta
    24301 cccgattcat ctttgaaatt agccagtggc ggcaaggcat tattttcatt cagtaacttt
    24361 gttagcgagt ttagttgctg acgatactga taatagccgg tcaggaattg ccacggtgcg
    24421 gcaggctcca tacgcgaggc caggttatcc aacgttttct caaacggctt gtttttgata
    24481 aacgtattca tggcgatcgg atgcagaatc aagccataaa gcagggcaaa agagacaaca
    24541 taacgccacg gctttggaat atagaccggg cgcaggcgtg tccacagcag aactgccacc
    24601 gccgtatagg ccagcgcgat aagcacaatt ttcaggctga aatactggct taaatactcg
    24661 ctggcttcgt tggtgttggt ttcgaacatc acaaacagaa cgctctgcga gaactcctgc
    24721 ccgtagatga cgtagtagca tagcgccgcc agagaggccg cccatagcac cacgccgatt
    24781 actgcggcaa taattttaat ccgcttcgga aagaggaata ccgggatcaa ccacagcgag
    24841 ctgaataaca gcgagtcgcg aatgccgttg gtgccactat aaccactgat gtaaataatg
    24901 gcctgtagca gagtagagaa aaaccaaaag tagagcagtg cccaacccag ggctttccag
    24961 ctaaaaaggg gtttagcctg gacttctgtg gaatgcatag taagaacctg tcttgaaaaa
    25021 aatatcgccg aatgtaacga caattcctta aggatatctg aaggtatatt cagaatttga
    25081 ataaaatgca gacagaaata tattgaaaac gagggtgtta gaacagaagt atttcagaaa
    25141 accctcgcgc aaaagcacga gggtttgcag aagaggaaga ttagccggta ttacgcatac
    25201 ctgccgcaat cccggcaata gtgaccatta acgcttgttc gacgcgagga tccggttcct
    25261 ggccttcttt ttctgcctgg cgggagcggt gcagcaactc ggcctgcaat acgttcagcg
    25321 ggtcggtgta aatattccgt agctgaatag actctgcaat ccacggcaga tcggccatca
    25381 gatgggaatc gttggcaatc gccagcacca ctttgatgtc ttcttcttgc aggttgcgta
    25441 actctttacc taacggccac agtgctttgt ctaccagacg ttgatcatag tattccgcca
    25501 accacaggtc tgctttggcg aagaccatct ccagcatgcc gagacgcgtc gagaagaatg
    25561 gccaatcgcg gcacatggct tccagctcgc tctgtttgcc atcttcgacc actttttgca
    25621 gcgccgtacc tgcacccagc caggcgggga gcatcaggcg gttttgcgtc caggcaaaaa
    25681 tccacggtat ggcgcgcagc gactcgacgc cgccggttgg gcgacgtttc gccggacgtg
    25741 aacccaacgg cagtttgccc aattcttgtt ccggcgtagc ggagcggaag taaggcacaa
    25801 aatctttgtt ttcacgtacg tagccacggt agagatcgca ggagatgact gacagttcat
    25861 ccataatgcg acgccagctc tctttcggct caggtggtgg cagcaggttg gcttccagaa
    25921 tcgccccggt gtaaagcgac aggctgctga cggtgatttc tggcagacca tatttaaagc
    25981 ggatcatctc gccctgttca gtcacgcgca ggccaccttt caggcttcct ggcggttgtg
    26041 acagcagcgc cgcatgggca ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga
    26101 acaacgtcag ctcaataccc gctttttcgc aggttttgat taatgcatcc tgtgcctgat
    26161 attgcgccca ggaagctgcc atcacgcccg catcttttgc tgagtcggaa tagccaatca
    26221 tcaccatctg tttgccctga atcaggccgc gataccagtc gatattgagc agctgggtca
    26281 tgacatcgtt ggcgttgttc aggtcatcga gggtttcaaa cagcggagca accggcatcg
    26341 caaacccgat acccgcttct ttcagcagca ggtggacagc cagtacgtcg gacggcgttt
    26401 tcgccatcga gatcacgtag gcggcaatgg agccttgcgg cgcttcggca atcacctggc
    26461 aggtatcgag cacttcgcgc gtttcggcgc ttggttgcca gttgcgcggc agaagcggac
    26521 gtttggagtt cagttcgcgg atcaggaacg cctgtttgtc ggcctctgac cagctttcgt
    26581 agtcgccgat accgaggtag cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct
    26641 cctgacggat atcaatacgg accagcggta cgccgaaaca tttcacgcgg cgcagggtgt
    26701 cgagcagatc gccgttggcg ataataccca tgccacacgc ctgaagtgac tggtagcaag
    26761 cgtagagcgg ttcccacagt tcttcgtttt gtgtcagcag cccttctggt tttggcagtt
    26821 cttcgccttt caggcgcgct tccagccatg cctgtgtcgc catcaggcga gaacgcaggt
    26881 ttttcatcag atagcgatac ggttctgcgg caccttcttc gccaaccagc gccagcagtt
    26941 caggggtcgc ttcaaccatc gacagttcag aaaccagaac ctgaatatct ttcaggaaca
    27001 gatcggtggc tttccagcga ctgagtagca ggacgtggcg ggtgatatcg gcagtgacgt
    27061 tcgggttgcc gtcgcggtca ccgcccatcc acgaagtaaa acggaccgga acaaattcga
    27121 cgggcagttt gtagccgagg ttctcttcca gttgttcgtt cagttcgcgc aagtaattcg
    27181 gtacgccttg ccacaggctg ttttctacta cggcaaagcc ccatttggct tcatctaccg
    27241 ggcttggacg cagcttacgg atttcatcag tatgccatga ctgggcgatt aactggcgca
    27301 ggcgacgcat cagctggttg tgttcgtagt cagcgatatc tttgttatcg agctgtttta
    27361 agcaggcgtt cacttccacc attttgtgga tcagtgtacg acgggtaatt tcggttgggt
    27421 gagccgtgag gaccagttcc agcgacagcg attccactgc ttttttgatg gtgtcttcgc
    27481 tcagttccgg ctggtttttc agtttgcgca gggtgcgggc gatcacttcc gggttgctgg
    27541 cagcttcgcc tttcggcgaa atgctgtggt attgctcggc ggtgttggcc aggttcagga
    27601 actgactaaa cgcacgcgca acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg
    27661 tgagcaactc ctggcggtta gcatcattgc cagcgcgtga agatttcgac aacttacgga
    27721 tagtttctac gcgttcaaga atgtgttctc ccaacgcatc cttgatggtt tctcccagca
    27781 ctttgccgag catactgaca ttactacgca atgcggaata ttgttcgttc atattacccc
    27841 agacacccca tcttatcgtt tgatagccct gtatccttca cgtcgcattg gcgcgaatat
    27901 gctcgggctt tgcttttcgt cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg
    27961 ctgcgaaatc gcttcagcaa acgaataaat agcaggaatt tacgccatta aattcacgac
    28021 gctttaaata agcgtaactt atggaaatgt taaaaaatcg ccatacataa caccaaaggt
    28081 gtaggtcgga taagatgcgc aagcatcgca tccgacatta ttgcggcacc ggagtttggc
    28141 aacggtgccg gatgcggcgc gagcgcctta tccggcctac gttttaatgc cagcaaaaat
    28201 ggtgaattac ctggataatc aactcacggg tgggtttaat aaaccgcgtc tccagatatt
    28261 catcaggctg atgagcttga ttgatggaac ccggaccgag caccagcgtc gggcataacg
    28321 tttgaataaa cggcgcttcg gtacagtaat tcaccacttc ggtttttgct ccgagcaatt
    28381 tctcaaccac ttcaaccagt tgatgattcg gtgggcattc atagccaggg atcggcggat
    28441 gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct caccggagcc aatgcatcgt
    28501 tgagcaaacc attaagttca ttgagtgtca tgctaggcag cggacgaata tccatatgca
    28561 actcacagca agcgcaaata cggttagaag cgtcgccacc gtgaatatgc ccgaggttga
    28621 gcgtagggta tggcacggta aatgcttcgt agtgataacg ttctttcagg ttatcgcgca
    28681 attgcaaaat atgcccgatg gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg
    28741 gatcgctgga gtgccccgac tggccctgaa tacggatggc gttagagata tgacctttat
    28801 gtgcgcgtac cggttgtagt gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg
    28861 cggtagtttc ggcaaaataa cgcgctccgg ccatactggt ttcttcatca gcagtcgcca
    28921 gaatgtagag cggttttttc agtttcgtga cgtcgacatc gcgtagcgta tcaaggataa
    28981 acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta aagcttgccg tcatgctccg
    29041 tcagtgtaaa cggatcgcgc gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc
    29101 ccgccagcaa caagccgcca gccccctgtc cgatacttgc cagcatattg aatttgttgc
    29161 gagttcctgg cacaggctgc acttccacat tgaagcctaa atctttaaac cagtccgcca
    29221 gcagagtgat taaatctgca ttgctttgat cgagtgcctc ttccgtggcg cttattgaag
    29281 gtgtggcaat cagagcacgg taaatctcga taaatggcgg taatttgttt ttcattgttg
    29341 acacacctct ggtcatgata gtatcaatat tcatgcagta tttatgaata aaaatacact
    29401 aacgttgagc gtaataaaac ccaccagccg taaggtgaat gttttacgtt taacctggca
    29461 accagacata agaaggtgaa tagccccgat gttgaatacg ctgattgtgg gtgccagcgg
    29521 ctacgctggc gcagagctag tgacctatgt aaatcgccat ccgcatatga acataaccgc
    29581 tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta atctccgatt tgcatccgca
    29641 gctaaaaggc atcgttgagc tgccgttgca gccgatgtcg gatatcagcg agtttagccc
    29701 aggggtggac gtagtgtttc tcgccaccgc ccatgaagtt agccacgatt tagcgccgca
    29761 gtttcttgaa gcgggctgcg tggtgttcga cctttccggt gcgtttcgtg ttaacgacgc
    29821 caccttctat gaaaaatatt acggctttac ccatcaatac ccggaactgc tggaacaggc
    29881 agcctacggt ctggcggagt ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt
    29941 gccaggctgt tatccgacgg cggcacagct ggcgctgaaa ccgttgattg atgccgatct
    30001 tcttgacctc aatcagtggc cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg
    30061 taaggcagcg atttcaaata gtttttgtga agttagcctg caaccgtatg gcgtctttac
    30121 tcatcgccat caaccagaga tcgccacaca cctcggtgct gacgttatct tcaccccaca
    30181 tctgggcaat ttcccgcgcg gcattctcga aaccattacc tgccgcctga aatcgggtgt
    30241 gacccaggcg caagtcgcgc aagtgttaca gcaggcgtat gcccataaac cgctggtgcg
    30301 gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat
    30361 cgggtttgcc gttcagggcg agcatctgat tattgtggcg accgaagaca acttactgaa
    30421 aggcgcggcg gcacaagcgg tacagtgcgc caatattcgt ttcggctatg cggaaacgca
    30481 gtctcttatt taagggtgca atgatgaatc cattaattat caaactgggc ggcgtactgc
    30541 tggatagtga agaggcgctg gaacgtctgt ttagcacact ggtgaattat cgtgagtcac
    30601 atcagcgtcc gctggtgatt gtgcacggcg gcggttgcgt ggtggatgag ctgatgaaag
    30661 ggctgaatct gccggtgaaa aagaaaaacg gcctgcgggt gacgcctgct gatcagatag
    30721 acattatcac cggagcactg gcgggaacgg caaataaaac cctgttggca tgggcgaaga
    30781 aacatcagat tgcggccgta ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc
    30841 agcttgatga agagttaggt catgttggac tggcgcagcc aggttcgcct aagcttatca
    30901 actccttgct ggagaacggt tatctgccgg tggtcagctc cattggcgta acagacgaag
    30961 ggcaactgat gaacgtcaat gccgaccagg cggcaacggc gctggcggca acgctgggcg
    31021 cggatctgat tttgctctcc gacgtcagcg gcattctcga cggcaaaggg caacgcattg
    31081 ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca gggcattatt actgacggca
    31141 tgatagtgaa agtgaacgcg gcgctggatg cggcccgcac gctgggccgt ccggtagata
    31201 tcgcctcctg gcgtcatgcg gagcagcttc cggcactgtt taacggtatg ccgatgggta
    31261 cgcggatttt agcttaagtt ttgttggccg gaggcgcagc tttccggcat tgaatttcaa
    31321 aataaggaaa cagagttatg gcactttggg gcgggcgttt tacccaggca gcagatcaac
    31381 ggttcaaaca attcaacgac tcactgcgct ttgattaccg tctggcggag caggatattg
    31441 ttggctctgt ggcctggtcc aaagccctgg tcacggtagg cgtgttaacc gcagaagagc
    31501 aggcgcaact ggaagaggcg ctgagcgtgc tgctggaaga tgttcgcgcc aggccacaac
    31561 aaatccttga aagcgacgcc gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca
    31621 aagtgggcca gttaggcaaa aagctgcata ccgggcgtag tcgtaacgat caggtagcga
    31681 ctgacctgaa actgtggtgc aaagataccg ttagcgagtt actgacggct aaccgacagc
    31741 tgcaatcggc gctggtggaa accgcacaaa acaatcagga cgcggtcatg ccaggttaca
    31801 ctcacctgca acgcgcccag ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga
    31861 tgctggcgcg tgatgaaagc cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat
    31921 taggctgtgg cgcgctggcg ggaacggcct atgaaatcga ccgtgaacag ttagcaggct
    31981 ggctgggctt tgcttcggcg acccgtaaca gtctggacag cgtttctgac cgtgaccatg
    32041 tgttggaact gctgtcggct gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag
    32101 atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga gctttctgac cgcgtgactt
    32161 ccggttcatc attaatgccg cagaagaaaa acccggatgc gctggagctg attcgcggta
    32221 aatgcggtcg ggtgcagggt gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc
    32281 tggcttacaa caaagatatg caggaagaca aagaaggtct gttcgacgcg ctcgatacct
    32341 ggctggactg cctgcatatg gcggcgctgg tgctggacgg cattcaggtg aaacgtccac
    32401 gttgccagga agcggctcag cagggttacg ccaacgccac cgaactggcg gattacctgg
    32461 tggcgaaagg cgtacctttc cgcgaggcgc accatatcgt gggtgaagcg gtggtggaag
    32521 ccattcgtca gggcaaaccg ctggaagatc tgccgctcga cgagttgcag aaattcagcc
    32581 cggtgattgg cgaagatgtc tatccgattt tgtcgctgca atcgtgcctc gacaagcgtg
    32641 cggcaaaagg cggcgtctca ccgcagcagg tggcccaagc gattgctttt gcgcaggcgc
    32701 ggttggggta agaacattta tatgtacaaa tttgagcctg gcttatcgcc gggctttttt
    32761 atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
    32821 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
    32881 tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg ctattctacc
    32941 tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta
    33001 cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagattcct gccacgttag
    33061 ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagctgggcg tgatgttgct
    33121 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
    33181 gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggcgagac
    33241 gatgtccgga ccgctgcata ttggtttgat tcccacggtt ggaccgtacc tgctaccgca
    33301 tattatcccg atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcaca
    33361 gacccaccag ttactggcgc aactggacag tggcaaactc gattgcgtga tcctcgcgct
    33421 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
    33481 tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ccgatagccg atctggcagg
    33541 ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg
    33601 ttttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg agacgctgcg
    33661 caacatggtg gcggcaggta gcgggatcac tttactgcca gcgctggctg tgccgccgga
    33721 gcgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat
    33781 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
    33841 catccgagca agaatggatg gctatttcga taaagtgtta aaacaggcgg tttaaaccgt
    33901 ttaacgcagc tacccgatag gcttccgcca tggtcgggta gttaaaggtg gtgttgacga
    33961 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
    34021 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
    34081 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcgcgtgcc agatgtttaa
    34141 actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt tcggttttgc
    34201 ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgcg
    34261 cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgctg
    34321 atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
    34381 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
    34441 acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag tcagctttca
    34501 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg atcttctcgt
    34561 actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag agagaatctg
    34621 acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta
    34681 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa
    34741 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat
    34801 gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt
    34861 cagcggttag tgtttcgacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa
    34921 caaagcgagc gttcccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca
    34981 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
    35041 ggagcagtcg cgaatggtcg ctgtatagtg ggttttgatt gaattctata atgcggctga
    35101 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
    35161 tttgataacg ctcgataact gcgacgcgca caccttgctt aaccaggccc attgcagcgc
    35221 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
    35281 tagggcttac ctgttcttat acataaaagc aacagaatgg taacattcta tcgcgggtaa
    35341 gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt
    35401 cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc
    35461 aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg ttaccgttca
    35521 ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt
    35581 ggcaaggatt cagacatcgt gatgggcata agagcgcaac aaaaagaaaa aacccgccgt
    35641 tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt
    35701 ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc
    35761 gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc
    35821 atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgcac ctcggtctcc
    35881 acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc
    35941 tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca cttcattgcg
    36001 gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa
    36061 gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc
    36121 gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg
    36181 gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag
    36241 gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg ttagttgctg
    36301 gcttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat
    36361 tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat cttaatcgca
    36421 ctatgccggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc gtactgctgt
    36481 acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac
    36541 tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg
    36601 ctgagtaatc ggtattatgc cggatgctgg cgcatccggc atgggtttta cttcgcggtc
    36661 agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt
    36721 tcgaccttgt gcgtctggct taatgtttcc agattcttgc ataacgtttc cgggttacag
    36781 gagatgtaca aaatacacgg atacgcctgc accattttct cggtttcact gtccagaccg
    36841 ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac tctttaagtc gatcccttgc
    36901 aggcggttaa actcgcgcac accattcatc gcctgagtaa attcttctgc cgccatacga
    36961 ataatttgta cgttatcaat atggttagct gcgatgttgt attgcgcagc agcaaccgac
    37021 ggcttagcga tttcggtggc taatacccga tcaaaattgc gcgccagcgc taatgaaaag
    37081 ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt tacgtccagc
    37141 gcccattcca gcatctgaat attcatcgcc gcgttcggct gggtaaagct gttttctacc
    37201 tgacggtaga tcatctcttt ccctgcgacc ggcagacgct cgtcgatgta atcttgatcc
    37261 agctcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgcgcgc
    37321 agtgcatcgc gcagggcctc cgcttcctga cgccactcat catccagctt cttatggtat
    37381 agcagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg
    37441 tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgata
    37501 agttcactgg cggcggggaa gctatccacg cggatacggc ttttggtttg ttgatcgaaa
    37561 atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcacg catccggtaa
    37621 tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt
    37681 tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg
    37741 ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag
    37801 atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa cttgttacaa
    37861 cctgtagcat ccacttgtcg gtcctgtgag ttaataggga atccagtgcg aatctggagc
    37921 tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg
    37981 ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa
    38041 cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta
    38101 ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt ctgtcacagc
    38161 cttttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg ctaaccgttt
    38221 tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga
    38281 ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac
    38341 ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca
    38401 tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg ggggtgagtg gttctgccga
    38461 ccttagccag ttccctattg cgcttgtcca gcgtgttgaa tatatccgtg ggccgcgctc
    38521 cgctgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga cgcgcgatga
    38581 acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc
    38641 tacgcagcaa caactggggg ataagacacg gggaacgctg ttgggcgatt atgcccatac
    38701 tcatggttat gatgttgttg cctatggtaa tacaggaacg caagcgcaga cagataacga
    38761 tggtttttta agtaaaacgc tttatggcgc gctggagcat aactttactg atgcctggag
    38821 cggctttgtg cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc
    38881 cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg
    38941 ctataacggc gaactgatta aatcacaact cattaccagc tatagccata gcaaagatta
    39001 caactacgat ccccattatg gtcgttatga ttcgtcggcg acgctcgatg agatgaagca
    39061 atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg gtgcgggtgt
    39121 cgactggcag aaacagacta cgacgccggg tacaggttat gttgaggatg gatatgatca
    39181 acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta cctttgaagg
    39241 cgcagcacgc agtgacgata actcacagtt tggtcgtcat ggaacctggc aaaccagcgc
    39301 cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat cttataaggc
    39361 accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc cggagaaaag
    39421 caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg
    39481 atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga aatattacaa
    39541 cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc aattttgata ccggaccact
    39601 gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg acacgccgtt
    39661 gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga
    39721 ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact catcttatcc
    39781 ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg cgtatccggt
    39841 cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac
    39901 agtctatggc taccaaactg caggacggga atacaccttg tctggcagct acaccttctg
    39961 aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg
    40021 agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc
    40081 cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt gtcaccgcgg
    40141 tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacactgcc agtaccgttt
    40201 cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta
    40261 aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag
    40321 ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa
    40381 tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctacatggc gaagatgttt
    40441 ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata
    40501 ccgttgtatt gggttgcacc catttccctc tactacaaga agaactgtta caagtgctgc
    40561 cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt
    40621 tagaacatga agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa
    40681 tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg
    40741 aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcagaaa attattttaa
    40801 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
    40861 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat ctcaacaga
//
LOCUS       DAJVXP010000018        40894 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVXP010000018 DAJVXP010000000
VERSION     DAJVXP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.018"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="OQS10_000970"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="OQS10_000970"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS2292068.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="OQS10_000971"
     tRNA            2260..2336
                     /locus_tag="OQS10_000971"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="OQS10_000972"
     tRNA            2345..2429
                     /locus_tag="OQS10_000972"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="OQS10_000973"
     tRNA            2453..2527
                     /locus_tag="OQS10_000973"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2543..2619
                     /locus_tag="OQS10_000974"
     tRNA            2543..2619
                     /locus_tag="OQS10_000974"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2577..2579,aa:Met,seq:cat)
     gene            2668..2742
                     /locus_tag="OQS10_000975"
     tRNA            2668..2742
                     /locus_tag="OQS10_000975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2700..2702,aa:Gln,seq:ctg)
     gene            2779..2853
                     /locus_tag="OQS10_000976"
     tRNA            2779..2853
                     /locus_tag="OQS10_000976"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2811..2813,aa:Gln,seq:ctg)
     gene            complement(3007..4182)
                     /gene="ubiF"
                     /locus_tag="OQS10_000977"
     CDS             complement(3007..4182)
                     /gene="ubiF"
                     /locus_tag="OQS10_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS2292069.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4328..5752
                     /gene="miaB"
                     /locus_tag="OQS10_000978"
     CDS             4328..5752
                     /gene="miaB"
                     /locus_tag="OQS10_000978"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS2292070.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5905..6945
                     /locus_tag="OQS10_000979"
     CDS             5905..6945
                     /locus_tag="OQS10_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS2292071.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            6942..7409
                     /gene="ybeY"
                     /locus_tag="OQS10_000980"
     CDS             6942..7409
                     /gene="ybeY"
                     /locus_tag="OQS10_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS2292072.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7499..8377
                     /gene="corC"
                     /locus_tag="OQS10_000981"
     CDS             7499..8377
                     /gene="corC"
                     /locus_tag="OQS10_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS2292073.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8402..9940
                     /gene="lnt"
                     /locus_tag="OQS10_000982"
     CDS             8402..9940
                     /gene="lnt"
                     /locus_tag="OQS10_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS2292074.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10018..11895)
                     /locus_tag="OQS10_000983"
     CDS             complement(10018..11895)
                     /locus_tag="OQS10_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS2292075.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12350..13258
                     /gene="gltI"
                     /locus_tag="OQS10_000984"
     CDS             12350..13258
                     /gene="gltI"
                     /locus_tag="OQS10_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS2292076.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13428..14168
                     /gene="gltJ"
                     /locus_tag="OQS10_000985"
     CDS             13428..14168
                     /gene="gltJ"
                     /locus_tag="OQS10_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS2292077.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14168..14842
                     /gene="gltK"
                     /locus_tag="OQS10_000986"
     CDS             14168..14842
                     /gene="gltK"
                     /locus_tag="OQS10_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS2292078.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14842..15567
                     /gene="gltL"
                     /locus_tag="OQS10_000987"
     CDS             14842..15567
                     /gene="gltL"
                     /locus_tag="OQS10_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS2292079.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15685..16620
                     /gene="rihA"
                     /locus_tag="OQS10_000988"
     CDS             15685..16620
                     /gene="rihA"
                     /locus_tag="OQS10_000988"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS2292080.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16712..18382
                     /gene="hscC"
                     /locus_tag="OQS10_000989"
     CDS             16712..18382
                     /gene="hscC"
                     /locus_tag="OQS10_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS2292081.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18442..19896)
                     /gene="djlC"
                     /locus_tag="OQS10_000990"
     CDS             complement(18442..19896)
                     /gene="djlC"
                     /locus_tag="OQS10_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS2292082.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19893..20600)
                     /locus_tag="OQS10_000991"
     CDS             complement(19893..20600)
                     /locus_tag="OQS10_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS2292083.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20702..21256
                     /locus_tag="OQS10_000992"
     CDS             20702..21256
                     /locus_tag="OQS10_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS2292084.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21266..22693)
                     /locus_tag="OQS10_000993"
     CDS             complement(21266..22693)
                     /locus_tag="OQS10_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS2292085.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22690..23397)
                     /locus_tag="OQS10_000994"
     CDS             complement(22690..23397)
                     /locus_tag="OQS10_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS2292086.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23561..24537
                     /locus_tag="OQS10_000995"
                     /pseudo
     CDS             23561..24537
                     /locus_tag="OQS10_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            complement(24607..25089)
                     /locus_tag="OQS10_000996"
     CDS             complement(24607..25089)
                     /locus_tag="OQS10_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS2292087.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25324..27906
                     /gene="leuS"
                     /locus_tag="OQS10_000997"
     CDS             25324..27906
                     /gene="leuS"
                     /locus_tag="OQS10_000997"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS2292088.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            27921..28502
                     /gene="lptE"
                     /locus_tag="OQS10_000998"
     CDS             27921..28502
                     /gene="lptE"
                     /locus_tag="OQS10_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS2292089.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28502..29533
                     /gene="holA"
                     /locus_tag="OQS10_000999"
     CDS             28502..29533
                     /gene="holA"
                     /locus_tag="OQS10_000999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS2292090.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29535..30176
                     /gene="nadD"
                     /locus_tag="OQS10_001000"
     CDS             29535..30176
                     /gene="nadD"
                     /locus_tag="OQS10_001000"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS2292091.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30200..30811
                     /locus_tag="OQS10_001001"
                     /pseudo
     CDS             30200..30811
                     /locus_tag="OQS10_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31071..31388
                     /gene="rsfS"
                     /locus_tag="OQS10_001002"
                     /gene_synonym="rsfA"
     CDS             31071..31388
                     /gene="rsfS"
                     /locus_tag="OQS10_001002"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS2292092.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31392..31859
                     /gene="rlmH"
                     /locus_tag="OQS10_001003"
     CDS             31392..31859
                     /gene="rlmH"
                     /locus_tag="OQS10_001003"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS2292093.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31890..33791
                     /gene="mrdA"
                     /locus_tag="OQS10_001004"
     CDS             31890..33791
                     /gene="mrdA"
                     /locus_tag="OQS10_001004"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS2292094.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33794..34906
                     /gene="mrdB"
                     /locus_tag="OQS10_001005"
                     /gene_synonym="rodA"
     CDS             33794..34906
                     /gene="mrdB"
                     /locus_tag="OQS10_001005"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS2292095.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            34917..36005
                     /gene="rlpA"
                     /locus_tag="OQS10_001006"
     CDS             34917..36005
                     /gene="rlpA"
                     /locus_tag="OQS10_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS2292096.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36144..37355
                     /gene="dacA"
                     /locus_tag="OQS10_001007"
     CDS             36144..37355
                     /gene="dacA"
                     /locus_tag="OQS10_001007"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS2292097.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37465..37728
                     /locus_tag="OQS10_001008"
     CDS             37465..37728
                     /locus_tag="OQS10_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS2292098.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37829..38470
                     /gene="lipB"
                     /locus_tag="OQS10_001009"
     CDS             37829..38470
                     /gene="lipB"
                     /locus_tag="OQS10_001009"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS2292099.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38730..39683
                     /locus_tag="OQS10_001010"
     CDS             38730..39683
                     /locus_tag="OQS10_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS2292100.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39892..40857
                     /gene="lipA"
                     /locus_tag="OQS10_001011"
     CDS             39892..40857
                     /gene="lipA"
                     /locus_tag="OQS10_001011"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS2292101.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10278 a        10415 c        10260 g         9941 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagaccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatcg aagaaacaat ctggctacgt agctcagttg gttagagcac atcactcata
     2581 atgatggggt cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac
     2641 gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc
     2701 tgattccggc attccgaggt tcgaatcctc gtaccccagc caatttattc aagacgctta
     2761 catcgtaagt gcaccagttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg
     2821 cattccgagg ttcgaatcct cgtaccccag ccacattaaa aaagctcgct tcggcgagct
     2881 ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt atccttcaat
     2941 gtcggatgcg acgctgccgc gtcttatccg acctacggtt ggcacgcatc cggcaatgtt
     3001 gtaaggctac aaccctaacg catatttcag cgcctgacgt ttcaacacgc cagcacgctc
     3061 tgccgccatt aacccgagat tacgcacaaa acgcagcggc ggcagattat tactgaatcc
     3121 ggcataaaac agatccatac cactttgcat aatgaagtta tccgccatgc gccgcatctg
     3181 gtaacgcttg aggacaggat aactggccca cgcttcaccg tagctgcggg cattcaccag
     3241 aacatcaatc agggcatcga catcacgata accaagattc accccctgcc ccgccagcgg
     3301 atggatggta tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat actgcaacgc
     3361 atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac ccagacgcga
     3421 cgggaaatgc ttcgcgattt ccgcctggaa ttgcgccata ttcatattct gcaactggcg
     3481 aatacgcgcc ggagagtcat accacaccag cgatgcccag ttatcaaaca acggcagaaa
     3541 cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg tcgccgggat cgttctcaca
     3601 ctgtacgcta atcaacatgc acgactgcgc atactgccag gcatgaacgc caattcccgc
     3661 catctgccgc acctgagaat ttgcgccgtc ggcaccaatc accagtttcg cgcggatcgt
     3721 ttcaccgccc ttcagttcca gctcctgaag atcattatct cgatgtaatg caatcagcga
     3781 gcctggcaca cgtaacgtta ctttcggatg cgcttccagc gcctgccaca gcgcctgttg
     3841 caggacggtg ttttccacca tatagccaag caacggtagc ttaagttcag cggcgtcaaa
     3901 caccacatgc gccgtttccc actcccacgt ttccagtctg cggtaaggat ggcaacgcat
     3961 agcctgtact gcatcccaga cccctaaccc tttaagcaat gataccgatg cggcgctgat
     4021 cgccgagatc cgcacgtccg gttggctatc agcgacaaac ggtgcaggtt ctgcgtgctc
     4081 gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc gccagcgcgc cgccgaccat
     4141 tcctccgccg acaatggcaa tttccgttgg ttgatttgtc atggtcaatc atcctgttag
     4201 caatatgctt taagtttaca ggatttttac ccccaagggc tgataacgct catactggtc
     4261 acaacggcag caaagcatta cactatacgc cctgcattcc tggctactat ttcgcaagag
     4321 caagtcgatg accaaaaaac tccatattaa aacctggggc tgtcagatga acgagtacga
     4381 ttcatcgaag atggccgatc tgctggatgc cacccacggc tatcaactga ccgacgtggc
     4441 ggaagaagcg gatgtgctgc tgctgaacac ctgctcaatc cgcgagaagg ctcaggaaaa
     4501 agtcttccat cagttgggtc gctggaaact gttaaaagag aagaatccag acctgattat
     4561 cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac attcgccagc gcgcccacta
     4621 tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg ccggagatga tcaactccgt
     4681 gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga agtttgaccg
     4741 tctaccggaa ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg aaggctgcaa
     4801 taaatattgc acctactgcg tggtgcctta cacccgtggt gaagaggtaa gccgtccgtc
     4861 tgacgatatt ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg aagtcaacct
     4921 gctcggtcag aacgtgaacg cctggcgtgg tgaaaactac gacggctcca ccggatcgtt
     4981 tgccgatctg ctgcgtctgg ttgctgcgat cgacgggatc gatcgtattc gctttaccac
     5041 cagccatccg atcgaattca ccgacgatat catcgaagtg tatcgcgaca cgccggagct
     5101 ggtgagcttc ctgcatctgc cagtacaaag cggttccgat cgcattctga acctgatggg
     5161 ccgtactcac acggcgctgg agtacaaagc gatcatccgt aaactgcgtg cggcgcgtcc
     5221 ggatattcag atcagttctg acttcattgt tggcttccct ggcgaaacca ccgaagactt
     5281 cgagaaaacg atgaagctga ttgccgacgt caatttcgac atgagctaca gctttatctt
     5341 ctctgcacgt ccgggtacac cagccgccga tatggttgat gatgttccgg aagaagagaa
     5401 gaagcaacgt ctgtatattc tgcaagaacg tatcaatcag caagcgatgg catggagccg
     5461 ccggatgctc ggcaccaccc agcgtattct ggtagaaggg acttcgcgta agagcatcat
     5521 ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca caccggatat
     5581 gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac ccgaactctc tgcgcggtaa
     5641 agtggtgcgt actgaagatg aaatgggtct gcgcgtggca gaaacaccgg aatcggtgat
     5701 tgcccgtacc cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt aattctcagg
     5761 cccgccgttc cggtgggcct tgttttttct ctctttatcc ccatctttat cgcaatgctt
     5821 gcgccttttt cctgtggcgt gaataatttc cttataggcc ggtgaatgtt cagcttccgg
     5881 cggcatacag ataagaggaa cggtttgaac atagacactc gcgaaatcac cctggagcca
     5941 gcagacaacg cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat caagcagctc
     6001 gaacgccgtc tcggcatcga gatcaatcgc cgcgacaacc actttaaact gaccggccgt
     6061 ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga tacagccccg
     6121 atgcgcggtc agattcagga tatcgaaccg gaacagatcc accttgcgat taaagaagcg
     6181 cgagtactgg agcaaagtgc ggagagcgtg ccggagtacg gcaaagcggt caatatcaaa
     6241 accaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat cgccaatatt
     6301 ctcgaccatg acattacctt cggcgttggc ccggcgggta cgggtaaaac ctacctggca
     6361 gtggctgcgg cagttgatgc cctggagcgt caggagattc gccgtattct gctgactcgt
     6421 ccggcggtcg aagccggtga gaaactgggc ttcctgcctg gcgatttaag ccagaaagta
     6481 gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt tgagaaagtt
     6541 gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat gcgtggtcgt
     6601 acgctgaacg acgcgtttat cattctcgat gagagccaga acaccaccat cgaacagatg
     6661 aagatgttcc tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg cgacgtcaca
     6721 cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc acgccatcga agtgctggcc
     6781 gatgtcgaag agatcagctt taacttcttc cacagcgaag acgtggttcg tcaccctgtg
     6841 gtggcgcgta tcgttaacgc ctatgaagcc tgggaagaag ccgaacaaaa acgaaaagcg
     6901 gcgctggcgg cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag gtgatcctcg
     6961 atttacaact ggcatgtgaa gataattccg ggttaccgga agagagccag tttcaaacat
     7021 ggctgaatgc ggtgatcccg cagtttcagg aagaatcgga agtgactatt cgcgtggtcg
     7081 ataccgccga aagccacagt ctgaatctga cctatcgcgg taaggataag ccgaccaacg
     7141 tgctctcctt cccgtttgaa gtgccgcctg gcatggaaat gtcgctactg ggcgatctgg
     7201 ttatctgccg tcaggtggtt gagaaggaag ctcaggagca aggcaaacca ctggaggcgc
     7261 actgggcgca tatggtggtg cacggcagtc tgcatttgtt aggttacgat cacatcgaag
     7321 atgacgaagc agaagaaatg gaagccctcg aaacagagat tatgcttgct ctgggctatg
     7381 aggatccgta cattgccgag aaagaataat ttgccaggca tttgactggc agacgtcccc
     7441 gccgttggtg attaacgacg acgttttgat taactaacat gagaacccat acgacgccat
     7501 gagcgacgac aattcacaca gtagtgacac gataagcaac aagaagggat ttttctccct
     7561 gttactcagc caacttttcc acggtgaacc gaaaaaccgt gacgaactgc tggcgctgat
     7621 ccgtgattcc gggcagaacg accttatcga cgaagatacg cgcgatatgc tcgaaggggt
     7681 gatggacatc gcagaccaac gcgttcgcga catcatgatc ccccgctccc agatgattac
     7741 cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc atcatcgagt ccgcccactc
     7801 acgtttcccg gtgattagcg aagacaaaga tcacattgaa gggattctga tggcgaaaga
     7861 cttgctgccg tttatgcgca gcgatgctga agccttcagc atggacaaag tgttacgtca
     7921 ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt ttcgctctca
     7981 gcgttaccac atggcgatcg tcattgacga attcggtggg gtttccggtc tggtgaccat
     8041 tgaagacatc ctggaactga ttgttggtga gattgaagac gagtatgacg aagaagatga
     8101 tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg cgtgcactgg cttccattga
     8161 agacttcaac gaagcgttcg gcacccactt tagcgatgaa gaggttgaca ctatcggtgg
     8221 tctggtgatg caggcatttg ggcatcttcc ggcgcgtggc gaaactatcg acatcgacgg
     8281 ttaccagttc aaagtggcga tggccgacag tcggcgtatt attcaggttc atgtcaaaat
     8341 cccggatgac tcaccccagc cgaagctgga tgaataaaac cgaaactgga tagataacta
     8401 catggctttt gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg cgttattatt
     8461 cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg cgattatttc
     8521 gttgatgggg cttcaggcgt tgacctttaa ccgccgtcca ctccagtctg ccgctattgg
     8581 cttttgctgg ggatttggcc tctttggcag cggtattaac tgggtctatg tcagcatcgc
     8641 gacctttggc ggaatgcctg gcccggttaa catcttcctg gtggtgctgc tggcggcgta
     8701 tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc cgaaaaccac
     8761 ctggctgcgc gtggcgattg ccgcccctgc tctctggcaa gtgaccgagt ttctgcgcgg
     8821 ttgggtactg acaggcttcc cgtggttaca gttcggctat agccagattg atggcccgtt
     8881 aaaagggctg gcaccgataa tgggcgtgga agccattaac ttcctgctga tgatggttag
     8941 tggcctgctg gcactggcgt tggtcaaacg caactggcgt ccgctggtgg tggccgtcgt
     9001 gctgtttgcc ctacccttcc cgctgcgtta catccaatgg tttaccccgc aaccggagaa
     9061 aaccattcag gtttcgatgg ttcagggcga tattccgcaa tcactgaaat gggatggaga
     9121 tcagcttctt aatacgctga agatttacta caacgcaacg gctccgctga tgggcaaatc
     9181 atcgttgatt atctggccgg agtcggcgat aaccgatctg gaaattaatc agcaaccgtt
     9241 cctcaaagca ctggacggtg agttgcgcga taaaggtagc tcgctggtaa ccgggattgt
     9301 cgacgcgcgt ctcaataagc agaaccgcta cgatacctac aacaccatca tcacactggg
     9361 taaaggtgca ccatacagct acgaatcagc cgatcgctat aacaaaaacc atctagtgcc
     9421 gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt tctttgatct
     9481 gccgatgtcg tcgttcagcc gtgggccata tatccagccg ccgctgtcgg taaatggtat
     9541 tgagcttact gcggctattt gctacgagat cattctcggc gagcaagtgc gcgataactt
     9601 ccgcccggat accgactatc tgctgactat ctccaacgat gcgtggtttg gtaagtctat
     9661 tggtccatgg cagcacttcc agatggcgcg aatgcgtgcg ctggagctgg cgcgcccact
     9721 gttgcgcagc accaacaacg gcattacggc ggtgattggc ccgcaggggg aggttcaggc
     9781 gatgatcccg cagttcaccc gcgaggtgtt gaccactaat gtgacaccga ccaccggact
     9841 cacaccgtat gcacgtaccg gcaactggcc actgtgggtg ctgacggcgc tgtttggttt
     9901 tgccgctgtg ttgatgagtc tgcgagcacg taaacgttga tgtgcctgat gtatcctgca
     9961 tgagcaaaac gtactttctc attgagcttt ggggcatggt tatcatgccc ctttttctta
    10021 cggttcaggg atacaagcca cattgccata ttggggatga cgaataattg cctgcggatc
    10081 aagcgagcgc gtcctgtcca tttggatcaa cccgttcttc gccatgacaa atatttttct
    10141 accatccgct gaaaaacgaa ggtgattaac gtagctggca ggataaacgc caataatctg
    10201 cccggcatta atattgagca ggatgacata accttgcagg ccatatgaca ccaacgctaa
    10261 atattgaccg tccgggctaa taccctcaac ggaataaacg ctcctgctac accattgcgt
    10321 cataatgctg gcttgttgat cccctacgct tatacgtgaa atcccctgac tatcggcaaa
    10381 aataacctcg ccgttttcag gtcgaaaacg catcgccgat atcgttccac gcgcatcacc
    10441 tgcgggaaag tcttctttct gatgcgtgat ggtattgatc cgcacgatgt tacctctgcc
    10501 tgctcccgga accagtaatg ttctttcatc cggggaaagt gccataaccc aggcgcctgg
    10561 tttaccgctc gtccccgtcc cgtcgtattt ctccagcggg atatccgcca catgctgata
    10621 agtcaccaga tcaatcgctg gcacactatt actggtgcga ttcatcaccc aggcttttgt
    10681 gccatcacgc gagaggataa ggctgtcagg atgtgcaccg gtggtaatcg actgaataat
    10741 ctcccctgtc gccacgttaa tgaccgataa cgcattcgct tcaaatgaag tcgcataaat
    10801 caacttttca tccgggctta atgccagcga tctgacgccc tgatctttac agccaccaca
    10861 atgtttgact gacttttctt tcgctatggg cgctgtaaag gtatgcagga ttttcttttg
    10921 tccgaggtcg aaaacaacaa tgctgttcgt gttttcaatt gcggcataca acatattacc
    10981 atcccgactg atcgccatat catggatatc ggcaatctca gcgagcaaat caccactggc
    11041 ctgttcacga cttaccggag cggataatgt ttccctgagg gcgttgcgtt cctccgctaa
    11101 ctgctgcttt ttttgccgtc gctcttgttc aatctcctgt ggataaaacg cttcacgcag
    11161 gctttgcctg acctgtaaaa ctgactcatc catctgctgc tgcgcaagcc agatagcccc
    11221 ggtcagaaga agactgcccg caacaataat cccgccttcc gtaacgagtc ggttttgccc
    11281 agataaacgc gcgctcagcc aacccaacgc gccacccgca atcaggccgc caatgtggca
    11341 agcgttatct attcctgttt gccgggcacc gtacaacagt gtcagcgcca ccatacccaa
    11401 cagaggaaat actcgccgct ggtttttatg gtatgtgccc gtacctgaag caaactgtgt
    11461 tgctatcgct gcaccagcta ttcccagaat cgccccggat gccccaacgc tgatgactat
    11521 ctgctcactt tcgcgtaacg cataacaggc gctgatgagc ccaccaaaga cgccggagaa
    11581 taaccaggta atcaggagtc gccatttccc cagaatctgt tccgccacgc ctcccacgac
    11641 caggaatgca aacatattca tcagcagatg agaaacacta ctgtgcagaa agatactgct
    11701 caataagcgc cagggttcac ccgacaaagt aagaggcgca acgtttgcgc cgagatggat
    11761 aagtgcggat tgatcgacct cttcataact catccattgt gcgagataac aaaatatccc
    11821 aatacagagt gcaaaaagga taagggttat tgcgggtaat tgaacattta acggcttaac
    11881 cgaagatgca gacataaggt gtttcttcct tgaaaagaca ggtaaaaagc atttttcccg
    11941 tgtgcagtta tcatcccggc aaacgtccca tgccgaaaag ataattctgt ccgaaatatg
    12001 actttttcag cctaaaatta caatcgtaat taattcgccc ctaagtattt tttggcatgt
    12061 aagttgcttt atttactgtg taagcgatga aatcagccgc gacgcaacca ggttgcacca
    12121 ccacaaatca gcggtagcac caaaaaggtg cggtttaaga attttgcttc ggaatggtgc
    12181 ggcgcatctc gcaaaaacaa acaataaaac agcaatatct ttacatttag ccaaactaaa
    12241 tgttacctaa caggcacaac actgcacaat aaagttgcag acgataacaa cacaaacact
    12301 cacaacgggt atccatgcgt tcttaacgca gaagataaag gagttggata tgcaattacg
    12361 taaacctgcc acagcaatcc tcgccctggc gctttccgca ggactggcac aggcagatga
    12421 cgccgccccg gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg tgattgtcgt
    12481 cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa aagtggtggg
    12541 ttactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc
    12601 ggacttgcag gtaaaactga ttccgattac ctcacaaaac cgtattccac tgctgcaaaa
    12661 cggcactttc gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc
    12721 ggctttctct gatactattt tcgtggtcgg tacgcgcctg ctgaccaaaa agggcggcga
    12781 tatcaaagat tttgccgacc tgaaagacaa agccgtagtc gtcacttccg gcactacctc
    12841 tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc
    12901 caaagatcac agtgactctt tccgcaccct ggaaagcggt cgtgccgttg cctttatgat
    12961 ggatgatgcc ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggaaat
    13021 cgtcggcaag ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag acgatccgca
    13081 gttcaaaaag ctgatggatg acaccaccgc gcaggtgcag acctccggtg aagcggaaaa
    13141 atggtttgat aaatggttca aaaatcccat tccgccgaaa aacctgaaca tgaatttcga
    13201 actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac tgaactaatt
    13261 acaagaacca ggggcggaaa ttccagccct ctcgattgtt acgtagcacg gacagactat
    13321 acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc
    13381 gagggtagca gttaacgctg ctaccctctt ttttctggag tagatttatg tctatagact
    13441 ggaactgggg tattttttta caacaagccc cgttcggcaa caccacctat cttggttgga
    13501 tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt atcgctttcc
    13561 tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc tccggtctgg
    13621 gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc tttacctggt
    13681 atctggtgat cccggagctg ttgccggaga aaatcggcat gtggtttaag gccgagctgg
    13741 atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt accgccgccc
    13801 gtgtttgcga acaggtacgc gccgccattc agtcgttgcc gcgtgggcaa aaaaatgccg
    13861 cgctggcgat gggcctgacg ctaccgcagg cttatcgata cgtactgctg cctaatgctt
    13921 atcgcgttat cgtcccgccg atgacttccg agatgatgaa cctggtgaaa aactccgcca
    13981 tcgcctcgac tattggtctg gtggatatgg cggcgcaggc gggtaaattg cttgattact
    14041 cagcccacgc ctgggagtcg tttaccgcta ttacgctggc gtatgtgttg attaacgcgg
    14101 ttatcatgct ggtaatgacg ctggttgaac gtaaagttcg cctgcctggc aacatggggg
    14161 gcaaataatg tacgagtttg actggagttc cattgtccct tccctgccat atctgctcga
    14221 cgggctggtg atcacgctga aaatcaccgt cacggcagtc gtgattggta ttttgtgggg
    14281 caccatgctg gcggtgatgc gcttgtccag ctttgcgccc gtagcgtggt ttgccaaagc
    14341 ctacgttaac gtattccgct cgattccttt agttatggtt ttgctgtggt tttacctgat
    14401 cgtgccaggt tttctgcaaa acgtgctggg attatcacca aaaaatgata tccgtttaat
    14461 ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga ttatccgtgc
    14521 tggtattcaa agtatttctc gtggtcagtc gagcgccgcg ctggcgttgg gaatgactca
    14581 ctggcagtcg atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg tgccgcttct
    14641 gctcactcag ggcatcgtac tgttccagga tacctcactg gtgtatgtgt tatctctggc
    14701 cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg ttgagatgat
    14761 cctgtttgcc ggatttgttt atttcgttat tagtcttagc gcgtcgttgt tggtcagcta
    14821 cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac
    14881 tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc
    14941 ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct cgaaccagtg
    15001 cagcaaggtg aaatcaccgt cgatggtatc gtggttaatg acaagaaaac cgatctggca
    15061 aagctgcgtt cccgcgtcgg gatggtattc cagcatttcg agctgttccc tcatctgtcg
    15121 attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcgataa agcgcccgct
    15181 cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt ccgcacatgc caataaattt
    15241 ccggcgcagc tttccggcgg tcagcagcag cgtgtggcaa tcgctcgcgc attgtgtatg
    15301 gatcctattg cgatgctgtt cgacgaaccg acatcggcgc tggatccgga gatgatcaac
    15361 gaagtgctgg acgtgatggt ggaactggcg aacgaaggaa tgaccatgat ggtggtgacc
    15421 cacgaaatgg gctttgcccg caaagtggcg aatcgggtga tctttatgga cgagggtaaa
    15481 attgtcgaag actcgccgaa agacgctttc ttcgatgatc cgaaatcgga ccgcgcgaaa
    15541 gacttcctcg cgaaaatcct gcattaatca ctctggcgcg catacccaag tgcgcgccat
    15601 ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa
    15661 agaagcacac aacaaggagc aacaatggca ctgccaattc tgttagattg cgacccaggt
    15721 catgacgacg ctatcgcaat agttctcgcc ctcgcctcac cagagcttga tgtcaaagca
    15781 attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg
    15841 ctgaccttgc ttaatcgcac cgatattccg gtagcaggcg gcgcggtaaa accgttaatg
    15901 cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg gtctggatgg cccggcatta
    15961 ccggaaccga cgttcgcacc gcaaaactgt acggcggtag agctgatggc gaaaacgctg
    16021 cgcgaaagtg cggaacctgt cacgattgtg tctaccggac cgcaaactaa cgttgccttg
    16081 ctgctcaata gccatccgga actgcatagc aaaattgccc gtatcgtgat tatgggcggc
    16141 gcaatggggc ttggtaactg gacgcctgcg gctgaattta atatttacgt tgacccggaa
    16201 gcggcagaaa ttgtcttcca gtcagggatc ccagtggtga tggccggtct ggatgttact
    16261 cacaaagcac aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg caaccctgtt
    16321 tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcataaaga cgaaaaatgg
    16381 ggctttgtcg gtgcaccgct gcatgaccca tgcaccatcg cctggctgtt gaaacccgag
    16441 ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aggggaaata tagccagggt
    16501 atgacggttg ttgattatta ttatctgact ggcaataaac cgaatgccac tgtaatggtc
    16561 gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt ttacgcttaa
    16621 cacacgtcat tgattaagat caccacgcgt tacctgggtg gtcttaatct gtttatctgt
    16681 ttatctcttt ttctttcaag atataaatgc aatggataat gcagaactcg ccattggtat
    16741 cgatctcggc actaccaata gtttaattgc cgtctggaaa gacggtgccg cgcaattaat
    16801 tccaaataag ttcggtgaat atttaacacc atccataatt agcatggatg aaaataatca
    16861 tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg catccggata aaacggcagc
    16921 gttatttaaa cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg acacatttaa
    16981 cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa gaagatgccg aagaatttct
    17041 gcaacgtccg attaaagatg tggtgatctc cgttccggct tatttcagcg atgaacaacg
    17101 caagcatacc cgtttagcag cggagttagc cgggttaaat gcggtacgct taattaatga
    17161 acccacagca gctgcgatgg catatggcct gcatacgcaa caaaataccc gctcgctggt
    17221 ttttgatctc ggtggcggca cgtttgacgt cacggtgctc gaatacgcca cgccggtgat
    17281 tgaagttcac gcctccgctg gcgataactt cctcggtggc gaagatttta cccatatgtt
    17341 ggtcgatgac gttttaaagc gcgcggatgt cgccaggacc acgcttaacg agagtgaact
    17401 ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc cattgcacat
    17461 tcgctggcag taccaggatg aaacgcggga gtgcgaattt tacgagaacg aactggaaga
    17521 tttgtggttg ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt tgcgcgatgc
    17581 acgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt cacaaatgcc
    17641 gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa gttacgaccc
    17701 gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac gcagtgaaga
    17761 tattgaagag gtgatcctca ctgatatttg cccttactcg ttgggcgttg aagttaaccg
    17821 ccagggcgtt tccggcattt tctcgccaat tattgaacga aacaccacag tacccgtgtc
    17881 gcgtgtagaa acttactcga ccatgcaccc ggaacaggat tctattacgg ttaacgtcta
    17941 tcagggagaa aaccacaaag ttaaaaataa cattctggtt gaatccttcg atgtgccgtt
    18001 gaagaaaacc ggggcttatc agtcgattga tattcgcttt agttatgata tcaacggctt
    18061 gcttgaagtt gacgtgcttc tggaagatgg cagcgttaag tccagagtca ttaatcatag
    18121 cccggtaaca ttgagcgcgc agcagattga agagagtcgg acgcggttat ccgcattgaa
    18181 aatttatccg cgcgatatgc taatcaaccg cacctttaag gccaaactgg aagagttgtg
    18241 ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg gtgatcaccg attttgatgc
    18301 ggcgttgcag tcaaacgata tggcccgcgt cgatgaagtt cgtcgacggg cgagcgatta
    18361 tttggctatt gagatccctt aaacacggct gccggatgcg gcataaacgc tgcatccggc
    18421 atcggcgcaa aaaacacatc ctcagctaaa caacatcgcg atttttccga gcgcataacc
    18481 tatcatcagc accgttaagc tgattttgat aatactggta atgctctgtg gtttttcatc
    18541 ctcatcattt ttttcttcat ggttttcagg tgttggctca tcgacatctt catcctccgg
    18601 ctcgagtttc actttcgtca acagcatctg ggtgataatg gcctctaatg gatgagtatc
    18661 tccaccttcc agccttgtat taagacgggc cagcatctct tgtttcggga acgcttccag
    18721 cacaacctgt tccgctaccc gatacaaatc cagtaacggt gaatcttgct ctgtctcgct
    18781 accgactctc gatttccacc acgcccatgc tttgcgcttg aagaataacg actcaataaa
    18841 cgattgacct aacggcgata attttccgct atgtaaaatc tccgcccata acagccgcgt
    18901 ccgggcgctc tcaccaggaa catatttcaa cggtttacct tgcgcatcta ccatgcttcg
    18961 cgcttcgata accatcacta ataacgcgaa ataatccggg caatgacgct tgctccactg
    19021 caacaacaaa ttatcagctt gtgtagatgg gtaactttcc cagtacgcgc agagatcggc
    19081 aagcaggctt tccccctcgc cgcagtacaa acgttgtgca cactggtaat accgggcatc
    19141 ttcgctttct ggttcttccg cttgccactg tttggctaca ggaatgagtt ccgccatacc
    19201 tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt aactttgcat catcaggaat
    19261 gaaccacggt ccttccatcg ccaaaaacgc agtgacatag tcaggatggt aacggcagca
    19321 acgttccagc gcgaagtaat attcaacagt ctggttctgt aacgcgacgg gatattccag
    19381 aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag cctccctttc
    19441 ttcctcatca tccattcctt cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa
    19501 caaagaagca caatttaatg aaagatatcg cgcttctata gcgattgcac acagcggcca
    19561 gcgtaattga tcgacatcct ccatgttcca ggtatttaat tgctgaataa atttctgcca
    19621 ggcggagggc tgaaaacgat cactttctga atccagtaat gtcctgaatg cacgtagtat
    19681 ttcatgttca gcggtggcat ctttttcttc atcatcggct tcctcgacag gatttaccgc
    19741 cagtcgcagc gcctcttcat aggcctggcg taatgcttta aacccctgcg gatcggtttc
    19801 gggatgacac aacggtaagc gagcaagata agcctggcgg ataatgtcta tttgcgtcgt
    19861 gctttcaatt tcgaggattt gccaacatgt tttcataggt cgctcacatc cttaagcgtt
    19921 accgggcatt caggctggac agttaatggt tgccagggaa gcgtggcata gaaacggtcg
    19981 cttcccgctg ccagctcttc gaacatccga ctcccggcgc tatcgtattt gtacagggtt
    20041 agcgcctcgc gtagcgaagc gttgtcttcg cactgagaag attgccagta gagacgccct
    20101 agtgaatagg cagaaaaata gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag
    20161 acgcgcgatt gcagccataa actctcttct tccgtcagcc agttgttgag cacaccgacc
    20221 cgtgacagaa accccattcg aacataatcc caggctttga ttcccccttc tccacagcac
    20281 atagcggttt gggcaacgaa ttgggtataa atcaaccctc tctccgagcc accagcaatt
    20341 aatgctttcc tctcttcagg ggaagagcgt tgccatgtca gatataagcc agctaaatca
    20401 ttggcatgac catcatcagt catctgataa atagtctgaa ttaaatcatc acgattattt
    20461 attccccagg atgatgagag atcgaattct tttatcccag tatacatttt tggcgagtca
    20521 tgcgcgggtt tgcaactctg attaagtatt tccatgggcg cagaaagcgc aaaaagaaga
    20581 tattgttctt ccttattcat tttttaataa tcccttaagt gcattaactg actacttttt
    20641 tattattgat gagtataaac ttgacgcttt caaaataaaa agaaaatcga agcattcaca
    20701 catgaataaa aaatcaatgt atatattcgc aatttttata gttgcagcaa ttacctgtat
    20761 tagccaaccc aagaaaacga cgttgcgtga taaagccatg gtgaattatg cctttgatta
    20821 tttaagatca ccaggcagtc taccgttaac cacggcagcc acggagcttt ccgcgattca
    20881 tggtcactca acatcgcaat atcgccttgg cgaattttat cttcatggta gcgacggtaa
    20941 accactggat tatacacagg cgagatactg gtatgagcaa tcagcagaac aggaaaatcc
    21001 gcgcgcacaa agcaaactgg ggtggatcta cctgaaaggc ctgggagtca aacccgatac
    21061 ccgtaaagca attctctggt ataaggaagc agctgaacaa gggtatgctc atgctcaata
    21121 tactttgggt ttgatctaca gaaatggcac tggtattaat gttaaccatt atgaatctca
    21181 aaaatggtta aaactggccg ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg
    21241 acttcccgca cattaacgtc gactgtcacc cgaagagatg gagtattttt cctatcaaac
    21301 cggcaaagat aaaaatgtag aaaataattt tgatgacgcc cagacagccc agcccgccat
    21361 cgtcaacggg cattggctgc ggttgcggct ccggctcgac ggtttcctga ccggtaaaat
    21421 tcgccgcctg aattaataat cgacgaataa ttaatgcttc cagtggacaa cgatcttctg
    21481 catcaaggcg gctcacaatc ggcattaatg attcttctgt cggttggctg gcctgcacta
    21541 attgtttggc aactaaatat aatggccata agggatgttc tgtatcacgc gtgagtgaga
    21601 tccgcggctt acgtcctttg tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga
    21661 gtttttcatc gctgagtatt tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga
    21721 cataaagtag ctgtttaccc tgatcgttaa ccagttcacg agcttcaatc atcatcacca
    21781 ctaacgggta ataatcgacc cgatgctgac ggcaccattg caatattaaa gcatccgcct
    21841 gggtggaggg atactcgcgc cagtagtcac aaagttctgc cagcaagctt tcgccttctc
    21901 cacaatagac gcgttgcgcg tattcataat aacgcgcatc ttcattatca ggttcagctt
    21961 cccgccacga ccgggcgtaa tccagaagtt cggggatatc caggcgacct gcgctatacc
    22021 agcggagtaa atttctgtgc agttttgcat catcggggat gatcactgcg cgatgttgcg
    22081 ctaaatacaa tgtcagccat tctggatgcg atgaccaaat ccgagcgaac atttggtaaa
    22141 aatcgatggt gtcattttgc acggcaacgg gcagatgcag aatggtctgg aagttaaaaa
    22201 cattcccctt cgcaatggca tataaaaagg attccagttc ttcttcgtct atttccccga
    22261 cgttttcctc ctgcaaccac cgcaatcttt ctgctaataa caccacgcac tcgaaggata
    22321 aatgggcagt gttcatggcg attgtgcaca gcgaccagcg taattcatca atctcttcca
    22381 tcgagcaata atttaattgc tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc
    22441 tatcagaggc aagtaacgcg cgaaaggcga gcagaatttc atgttctgct acctcatatt
    22501 cttctggttg ccaaacagat ttagcaggcg actgcgcaat ccggagcgcc tcctcatacg
    22561 cctgacgaag ttgtttaaaa ccctgcggat cggtttctgg atgaaaggac agtaacagcg
    22621 ccagataagc gcggcggata atatcgacgt cagtcgtttc ctctatttcg aggatcttcc
    22681 agcaattttt cataggtcgc tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc
    22741 cagtggttgc caggacaagg cagggtaata cgcatcatca ctggcaaata attgataaaa
    22801 catatcattg ccggaatcgt cgtattcttt acgagctaag aattcgcgta ataaaggcag
    22861 atgatcatct tcgggagact gccaatattg ccgaccaaag gtataaccgg caaagtattg
    22921 ccgccagtta ctataataac gtagagcccg tagatgaata cgcgactgga tccatagact
    22981 ttcttcttca ctcaaccagt tattaagtac gcccattcga cttaaaaaac ccatccggac
    23041 ataatcccat gctttaattc ctccctcacc acagcattcg gctgtgctgg cgacaaattg
    23101 cgcataagcc tgaccttgct catttagttc agcaagatag tcacgccatt cacatggcga
    23161 ataacgaaac cagcggtgat aaaaccctgc caaccgagcg gcatgaccgt tatcgatcat
    23221 tcggtgaatt gtgcgcagca ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc
    23281 aaaaaagcat gtacctaaat acattttggg ggatgaatgc gaaggtaaac agcattcatt
    23341 gcgtatttcc atcggagtgg aaagtgcaaa caatattttt tgcgacccca tgtccatata
    23401 atttatcctt aaacattcat ttaatgaatg taaactacat ttcgagactc agttatttat
    23461 acttttctga cgcaacataa tacgcataaa ataaggctta cgcaaaaatg gaacacataa
    23521 aaatataata tgttttatct acaaccataa ggaaatgata atggttttta cgtcaagttg
    23581 ctgcgataat ttatcaatag atgagattat cgaacgtgct gaaaaaggcg attgcgaggc
    23641 tcaatatatt gttgggtttt attataatcg cgatagcgca attgactctc cggacgacga
    23701 aaaggccttt tactggctga agctggccgc tgagcaaggt cattgtgaag cacagtattc
    23761 cttagggcaa aagtataccg aggataaaag ccgtcataaa gataatgagc aagccatctt
    23821 ctggctgaaa aaagctgccc tacaaggcca tactttcgct tccaacgccc ttggctggac
    23881 actggatcgt ggagaagccc ccaattataa agaagcggtt gtctggtatc agatagccgc
    23941 ggagagcgga atgtcttatg cgcaaaataa tcttgggtgg atgtacagaa atggcaacgg
    24001 agtcgcaaaa gactatgcgc tggcattttt ctggtacaaa caagctgcat tacaaggcca
    24061 tagtgacgcg caaaacaatc tggccgatct ttatgaagac ggaaaaggcg ttgctcaaaa
    24121 caagacactc gccgcattct ggtatttgaa aagcgcacag cagggtaatc ggcacgccca
    24181 gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg gaccaggact ataagcaagc
    24241 gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg
    24301 ttatatgtat aaacacggac aaggcgttga gaaagattat caggctgcct ttgaatggtt
    24361 tacgaaagcc gctgaatgca atgacgccac tgcctggtat aacctggcca ttattatcac
    24421 tacggagaag gaagacctgt cgatctccga caggctctcg acctgtatcg taaagttcag
    24481 tcatccggaa ccagagatgt cagtcaggaa attcgtgaga ctgaagattt actgtagaga
    24541 taaaaacaag ggccggggaa tttcattcgg cccatattct ctttattgct gtgcgaaatc
    24601 agactattac ggctcaaacg ggcgtctctg gaactggtca tgaccacatt tcgggcatag
    24661 cgtcagcact tccggtgtgt agatcgggag atggaagtga catttctcgc agaccagatt
    24721 tcccagcccg accacttctc cgctgtgata aaccccatga tgattgaggt cctggaaaac
    24781 ttcgcgccat tcaagctgcg ttttatcggt gatatctgcc agctcctgcc ataagctttc
    24841 tttaatcacc cgcataaaga cgctgtcaga ttcttctttc aggctctctt catagctcat
    24901 ggcgaactct tccaggtcac gtcttacagc tcgcgtcagc tcatcgacct cggttcgcgt
    24961 taactcccct gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg
    25021 ttcgccattg cgcaggcgtt cgctcagtga cgcgaccagt tcacggtaat attgagcaac
    25081 cttgttcatc gtttcatctc ccgggtcgtt aactgactat aataatagac gttctttgtt
    25141 ccttgctttg ctaatacgcc cacaaactct gtaaattaat gtatgaataa tccgggcatc
    25201 atttcaacgt cgagtgcgcg aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg
    25261 gatctgaaaa accacctcaa cgctacattt gtagccgtat tgaaaacagg accactggct
    25321 gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat
    25381 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
    25441 atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt
    25501 gacgtgatcg cccgctacca gcgtatgctg ggcaaaaacg tcctgcagcc gatcggctgg
    25561 gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg
    25621 tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat
    25681 gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc
    25741 ttcaccgagc tgtataaaaa aggcctggta tataagaaga cttctgcggt caactggtgc
    25801 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
    25861 gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct
    25921 gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt taaaaccatg
    25981 cagcgtaact ggatcggtcg ttccgaaggc gtggagatca ccttcaacgt taacgactat
    26041 gacaacatgc tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg
    26101 gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc tgaactggcg
    26161 gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag
    26221 aaaaaaggcg tcgatactgg ctttaaagct gttcacccat taacgggcga agaaattccc
    26281 gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagttat ggcggttccg
    26341 ggtcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccagtt
    26401 atcctggctg ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
    26461 gtgctgttca actctggcga attcaacggt cttgaccatg aagcggcctt caacgccatc
    26521 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac
    26581 tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac gctggaagac
    26641 ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg
    26701 atggacggca ttaccagccc gattaaagca gatccggagt gggcaaaaac taccgttaac
    26761 ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac
    26821 tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga agcggctaac
    26881 tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat gcacctgctc
    26941 tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca
    27001 gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa
    27061 aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
    27121 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
    27181 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
    27241 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
    27301 gaatccggtg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtttacgag
    27361 cacacagcaa aaggtgatgt tgcggcactg aacgttgatg cgctgactga agatcagaaa
    27421 gcgctgcgtc gcgatgtgca taaaacgatc gctaaagtga ccgatgatat cggccgtcgt
    27481 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
    27541 ccaaccgatg gcgagcagga tcgcgctctg atgcaggaag cgctgctggc cgttgtccgt
    27601 atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa
    27661 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
    27721 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcca aaatcaccgt tctggtggac
    27781 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
    27841 gatggcgtta ccgtgcgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt
    27901 ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct ctggcggtgt
    27961 taatcaccgc cgggtgtggc tggcatctgc gtgataccac gcaggttcct tccactatga
    28021 aggtcatgat cctggactca ggcgatccga acgggccatt aagccgtgca gtgcgtaacc
    28081 agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat
    28141 ccctgcgttt gggtaaagtg agcatcgcga aagataccgc atcggtattc cgtaacggtc
    28201 aaacagcaga gtatcagatg atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg
    28261 atatctaccc gattagcgcc aaagtcttcc gttcgttctt tgataacccg caaatggcgt
    28321 tagcgaaaga taacgaacaa gacatgatcg taaaagagat gtacgaccgt gctgccgaac
    28381 agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga
    28441 cgtcgaccac aacggatact ccggcaacgc ctgcacgcgt ctccaccacg ctgggtaact
    28501 gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc
    28561 gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg ctgttcgtca
    28621 ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc ccaacactga
    28681 ctggaatgcg atcttttcgt tatgccaggc tatgagtctg tttgccagtc gacaaacgct
    28741 attgctgttg ttaccagaaa acggaccgaa tgcggcgatc aatgaacaac ttctcacact
    28801 caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggtaataaat taagcaaagc
    28861 gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca
    28921 gacaccggag caggctcagc ttccccgctg ggttactgcg cgcgcaaaac agctcaactt
    28981 agaactggat gacgcggcaa atcaggtgct ctgctactgt tatgaaggta acctgctggc
    29041 gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaat tgacattacc
    29101 gcgcgttgaa caggcggtga atgatgccgc gcatttcacc ccttttcatt gggttgatgc
    29161 tttgttgatg ggaaaaagta agcgcgcatt gcatattctt cagcaactgc gtctggaagg
    29221 cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctgttgttac tggttaacct
    29281 gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc gggtatggca
    29341 gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagacgc agttacgtca
    29401 ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattacg gtcagtcagt
    29461 gtgggcagag ctgaaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt
    29521 tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc
    29581 actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgggtca
    29641 caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac agcgtgcagc
    29701 gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg
    29761 agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggaac
    29821 aaggaccgga cgtgccgctg gcgtttatta ttggtcagga ttcactgctg acctttccga
    29881 cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtccag
    29941 gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat catttgacac
    30001 ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt
    30061 ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa tcgtgtgagg
    30121 atttattgcc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat cgctgatacc
    30181 tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggtgaaa cgcaagcgaa
    30241 tatcgatggt ctttacagcg gtcatgcgcc cacccccctg accgcgcgcg gtattgagca
    30301 agcgcaaaat ctgcatacgc tgctacatgg tgtttccttt gatctggttt tatgcagtga
    30361 actggaacgg gcacagcata ccgcacgact ggttctcagt gaccgccagc tccccgtgca
    30421 aatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac atcatcgcga
    30481 cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgcaat
    30541 ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag
    30601 gcttagtgaa tttcagcact atcagaatat tttagtcgtc agccatcagg gtgtactgag
    30661 tctgttaatc gcccgtttaa ttggcatgcc tgccgaggct atgtggcatt ttcgcgttga
    30721 ctaggggtgt tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa
    30781 tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg gtaagccgca
    30841 acgaccattg acagacccgg gcaggctgat attctccgca gccagacttt tttcgccaga
    30901 cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga
    30961 tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca
    31021 ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta
    31081 aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg
    31141 ccttagacgt tcaaggcaaa tccagcatca ccgactgcat gatcatctgt acgggtacgt
    31201 ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc gcagcgggcc
    31261 tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg
    31321 atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct
    31381 ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac
    31441 aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg
    31501 aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg
    31561 gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca
    31621 agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg
    31681 acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg
    31741 agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg
    31801 cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat
    31861 aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata
    31921 cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc
    31981 tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc
    32041 agactcgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc cgcggcatta
    32101 tctacgaccg taatggtatc cctctggccc tcaaccgcac tatctaccag atagaaatga
    32161 tgccagagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggttgatc
    32221 tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac cgtttcacct
    32281 ctattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc gtcaatcagt
    32341 accgttttcc gggtgtcgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg
    32401 cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga
    32461 ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag ctgggcattg
    32521 agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca
    32581 accgtgggcg tgttatccgc cagttaaaag aagtaccacc gcaagccgga cacgatattt
    32641 acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg
    32701 cagctgtggt tgtcaccgat ccgcgtacag gtggggtgct ggcgctggtt tccacgccta
    32761 gttatgaccc aaacctgttt gttgacggta tctccagcaa agattattcc gccttgttga
    32821 acgccccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta
    32881 cagttaaacc ctatgtggcg gtttcggcat tgagcgccgg ggtgatcacg cgcaatacga
    32941 cgctgtttga cccaggctgg tggcaactgc caggttcgga aaaacgttat cgtgactgga
    33001 aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct
    33061 tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atgggtaaat
    33121 tcggttatgg tcattacacc ggtatcgacc tggcggaaga acgttccggc aacatgccta
    33181 cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg
    33241 ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga
    33301 tcctgattaa tgacggtatc gtgaaggttc ctcatttgct gatgagcacc gccgaagacg
    33361 gcaaacaggt gccatgggta cagccgcatg aaccgcccgt cggcgatatt cattccggtt
    33421 actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac ggtacggcgc
    33481 ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atccggtacc gctcaggtct
    33541 tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc
    33601 acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc
    33661 tggagaacgg tggtgcgggt ccggcggttg gtacactgat gcgccagatc ctcgaccaca
    33721 ttatgctggg tgataacaac accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg
    33781 aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata aagtccatct
    33841 cgatcccaca atgctgctga tcttactggc attgctggtt tacagcgccc tggttatctg
    33901 gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg
    33961 tctagtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc
    34021 ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg gtgccatctc
    34081 taaaggtgct caacgctggc tggacctcgg tattgttcgt tttcagccgt cggaaattgc
    34141 caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc
    34201 gttgaagaac actggcatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc
    34261 acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg tactgttcct
    34321 ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtggcgg cgttcattcc
    34381 gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga tgctcctaga
    34441 cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa ttgctattgg
    34501 ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct
    34561 ccccgaacgc cataccgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg
    34621 cattctgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc
    34681 cagagcgcaa accacctttg gtcgcgtcat ggctggtggc ttaatgctga tattattcgt
    34741 ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct
    34801 cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt
    34861 aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc
    34921 gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg
    34981 atggtcagca acagacggta agtgtaccgc agcctgcggt atgtaacggc cccatagttg
    35041 aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc
    35101 agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttagc caggcgggac
    35161 tggcggcaat ctatgatgcc gaacccggca gtaacctgac ggcctctggc gaagctttcg
    35221 atccgacaca gctgacggcg gcccatccaa cgcttccgat ccccagctac gccagaatca
    35281 ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc ccttacggca
    35341 acgaccgcgt tatttcactt tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca
    35401 ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta
    35461 tggcttgtac cacagtcgcc aaacagactt acgccctgcc agcacctccc gatttaagcg
    35521 gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg gtcagtaatt
    35581 cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagtagcggt ttcctcggcg
    35641 ctccaacgac cttagcgcct ggtgtactgg aaggcagcga accgacgcct gcgccacagc
    35701 ccgttgttac agctccgtcg acaacgcctg caacctcgcc tgcaatggtg acaccgcaag
    35761 ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt cggggccgta agcgatcagg
    35821 ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa
    35881 ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccagcaaa gccgaagcca
    35941 gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt actaccgcgc
    36001 agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg
    36061 cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt tttaattcca
    36121 tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc
    36181 tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga
    36241 atatcaaaac tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgattg
    36301 actataactc cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca
    36361 gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta
    36421 aagaaactga tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta
    36481 aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc
    36541 gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg
    36601 gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgcactg ggcctgaaaa
    36661 atacccactt ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag
    36721 atatggcgct gatcggccag gcattgatcc gtgacgtacc gaatgaatac tcgatctata
    36781 aagaaaaaga atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg
    36841 ataacagcct gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc
    36901 ttgttgcttc tgcgactgaa ggccagatgc gcttgatttc tgcggtgatg ggcggacgta
    36961 cttttaaagg ccgtgaagcc gaaagtaaaa aactgctaac ctggggcttc cgtttcttcg
    37021 aaaccgttaa cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg
    37081 attctgatcg cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccacgtggtc
    37141 gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc
    37201 aaaagaatca ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgagcaac
    37261 gcccgctggt tgtgttgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt
    37321 acattaaatt aatgttccat cactggtttg gttaaaaatt gaacacttga aagtgtaatt
    37381 tccgtcccca tatactaagc atcagtaaaa aactcccgcc ttctggcggg agttgctatt
    37441 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    37501 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    37561 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    37621 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    37681 ctgtatgaag aactaggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    37741 ttacccggcc cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    37801 tttactcatt ctccacggag atgcagtttt gtatcaggat aaaattcttg tccgccagct
    37861 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
    37921 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaaggtca
    37981 ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    38041 cggtgggcag gtgacttatc acgggccggg gcaacaggtg atgtatgtgt tgcttaacct
    38101 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    38161 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
    38221 cggggaaaag aaaatttgct cactgggttt acgtattcga cgcggttgtt cattccacgg
    38281 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    38341 tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    38401 tgcaccacgt ttactggaaa atattttagc gctactaaac aatcctgact tcgaatatat
    38461 taccgcttaa ttgcatacct caatggccca atttacactg ggtcattact cataatacct
    38521 gctaatgctt tacatctcct tcgcgtttgt atactttctt tatcacttgc agaaatgcgg
    38581 tgctacgcct tgctccgtaa ctcaccccat tgattaacct attgaaatag ttgattatgg
    38641 tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt taaatattat
    38701 ttttccatga ataaaattgg agtaagtgcg tggatagtaa taatcaaatt gaaccctgtt
    38761 taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac aaccttacga aatattgatc
    38821 ttaaccttct gactattttt gaagctgtat atgtacataa agggatcgtt aatgcagcga
    38881 aagtgcttaa tctgaccccc tcggcaatca gtcagtctat tcagaaactg cgcgttatat
    38941 tccctgaccc attgtttatt cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc
    39001 atctacatga gtatatcagt cagggccttg agtccattct tggcgcgctg gatatcgaag
    39061 gaagctatga taagcaacga acgataacta ttgcgaccac tccctcggtc ggagccctgg
    39121 tccttcctgt catctatcgg gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc
    39181 cgcccatcag cgacgcggaa aaccaactca gtcagtttca aaccgatctc atcatcgata
    39241 acatgttttg caccaatcgt acggtgcaac atcatgttct gttcaccgac aatatggtgt
    39301 taatttgccg cgagggaaat ccactactct ctttagaaga tgacagagag actatcgaca
    39361 acgctgcgca tgtactcctg ttaccggaag ggcaaaattt cagcggtctg cggcagagag
    39421 ttcaagagat gtttccggac cggcaaatca atttcaccag ctacaacatt ttgacaatcg
    39481 ctgcactggt tgccaacagt gacatgttag cgattattcc cagccgtttt tataacctgt
    39541 ttagtcgttg ctggccgctg gaaaaactgc cttttccgtc cttaaatgag gagcaaatag
    39601 atttctctat ccactacaac aaattcagcc tgcgtgatcc catcctgcac ggggtaatag
    39661 atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag cgcagaacga
    39721 taaaatggca caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg
    39781 tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc
    39841 tattgaattg aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa
    39901 cccattgtga tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg
    39961 gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc
    40021 aagcttccag cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc
    40081 ctgcattctg tctgcgagga agcctcctgc cctaacctgg cggaatgctt caaccacggc
    40141 acagcaacgt ttatgatcct cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt
    40201 gcccacggtc gcccggtagc tcctgatgcc aatgaaccag tgaaactggc gcagaccatt
    40261 gccgatatgg cgctgcgtta tgtggttatc acctccgttg accgtgatga cctgcgcgat
    40321 ggcggtgccc agcactttgc ggattgcatt actgccattc gggaaaaaag cccgcaaatc
    40381 aaaattgaaa ctctggtgcc ggatttccgt ggtcgtatgg atcgtgctct ggatattctg
    40441 actgcaacgc caccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgt
    40501 caggtacggc ctggtgcaga ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa
    40561 gcgcatccgg aaatcccgac caagtctggt ctgatggtgg gactgggtga aaccaatgaa
    40621 gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg
    40681 caatatttgc agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag
    40741 ttcgacgaaa tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg
    40801 tttgtccgct cttcttacca cgccgatttg caggcgaaag ggatggaagt taagtaatcc
    40861 tgtaataatt acttacatct gtctgataaa aaac
//
LOCUS       DAJVXP010000019        39788 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVXP010000019 DAJVXP010000000
VERSION     DAJVXP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.019"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(93..168)
                     /locus_tag="OQS10_001012"
     tRNA            complement(93..168)
                     /locus_tag="OQS10_001012"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Lys,seq:ttt)
     gene            complement(172..247)
                     /locus_tag="OQS10_001013"
     tRNA            complement(172..247)
                     /locus_tag="OQS10_001013"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(212..214),aa:Val,seq:tac)
     gene            complement(397..472)
                     /locus_tag="OQS10_001014"
     tRNA            complement(397..472)
                     /locus_tag="OQS10_001014"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(437..439),aa:Lys,seq:ttt)
     gene            complement(637..1428)
                     /gene="cpoB"
                     /locus_tag="OQS10_001015"
     CDS             complement(637..1428)
                     /gene="cpoB"
                     /locus_tag="OQS10_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS2292102.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1438..1959)
                     /gene="pal"
                     /locus_tag="OQS10_001016"
     CDS             complement(1438..1959)
                     /gene="pal"
                     /locus_tag="OQS10_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS2292103.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1994..3286)
                     /gene="tolB"
                     /locus_tag="OQS10_001017"
     CDS             complement(1994..3286)
                     /gene="tolB"
                     /locus_tag="OQS10_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS2292104.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3419..4651)
                     /gene="tolA"
                     /locus_tag="OQS10_001018"
     CDS             complement(3419..4651)
                     /gene="tolA"
                     /locus_tag="OQS10_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS2292105.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4716..5144)
                     /gene="tolR"
                     /locus_tag="OQS10_001019"
     CDS             complement(4716..5144)
                     /gene="tolR"
                     /locus_tag="OQS10_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS2292106.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5148..5840)
                     /gene="tolQ"
                     /locus_tag="OQS10_001020"
     CDS             complement(5148..5840)
                     /gene="tolQ"
                     /locus_tag="OQS10_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS2292107.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5837..6241)
                     /gene="ybgC"
                     /locus_tag="OQS10_001021"
     CDS             complement(5837..6241)
                     /gene="ybgC"
                     /locus_tag="OQS10_001021"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS2292108.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6391..6684)
                     /gene="ybgE"
                     /locus_tag="OQS10_001022"
     CDS             complement(6391..6684)
                     /gene="ybgE"
                     /locus_tag="OQS10_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS2292109.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6684..6797)
                     /gene="cydX"
                     /locus_tag="OQS10_001023"
     CDS             complement(6684..6797)
                     /gene="cydX"
                     /locus_tag="OQS10_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS2292110.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6812..7951)
                     /gene="cydB"
                     /locus_tag="OQS10_001024"
     CDS             complement(6812..7951)
                     /gene="cydB"
                     /locus_tag="OQS10_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS2292111.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7967..9535)
                     /gene="cydA"
                     /locus_tag="OQS10_001025"
     CDS             complement(7967..9535)
                     /gene="cydA"
                     /locus_tag="OQS10_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS2292112.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10131..10358)
                     /locus_tag="OQS10_001026"
     CDS             complement(10131..10358)
                     /locus_tag="OQS10_001026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292113.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10383..13016)
                     /gene="mngB"
                     /locus_tag="OQS10_001027"
     CDS             complement(10383..13016)
                     /gene="mngB"
                     /locus_tag="OQS10_001027"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS2292114.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13034..14950)
                     /gene="mngA"
                     /locus_tag="OQS10_001028"
     CDS             complement(13034..14950)
                     /gene="mngA"
                     /locus_tag="OQS10_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS2292115.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15119..15841
                     /gene="mngR"
                     /locus_tag="OQS10_001029"
     CDS             15119..15841
                     /gene="mngR"
                     /locus_tag="OQS10_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS2292116.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15945..16814)
                     /gene="sucD"
                     /locus_tag="OQS10_001030"
     CDS             complement(15945..16814)
                     /gene="sucD"
                     /locus_tag="OQS10_001030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS2292117.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16814..17980)
                     /gene="sucC"
                     /locus_tag="OQS10_001031"
     CDS             complement(16814..17980)
                     /gene="sucC"
                     /locus_tag="OQS10_001031"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS2292118.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18074..19291)
                     /gene="odhB"
                     /locus_tag="OQS10_001032"
     CDS             complement(18074..19291)
                     /gene="odhB"
                     /locus_tag="OQS10_001032"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS2292119.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19306..22107)
                     /gene="sucA"
                     /locus_tag="OQS10_001033"
     CDS             complement(19306..22107)
                     /gene="sucA"
                     /locus_tag="OQS10_001033"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS2292120.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22408..23124)
                     /gene="sdhB"
                     /locus_tag="OQS10_001034"
     CDS             complement(22408..23124)
                     /gene="sdhB"
                     /locus_tag="OQS10_001034"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS2292121.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23140..24906)
                     /gene="sdhA"
                     /locus_tag="OQS10_001035"
     CDS             complement(23140..24906)
                     /gene="sdhA"
                     /locus_tag="OQS10_001035"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS2292122.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24906..25253)
                     /gene="sdhD"
                     /locus_tag="OQS10_001036"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24906..25253)
                     /gene="sdhD"
                     /locus_tag="OQS10_001036"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS2292123.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25247..25651)
                     /gene="sdhC"
                     /locus_tag="OQS10_001037"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25247..25651)
                     /gene="sdhC"
                     /locus_tag="OQS10_001037"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS2292124.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26032..26139
                     /locus_tag="OQS10_001038"
     CDS             26032..26139
                     /locus_tag="OQS10_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292125.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26345..27628
                     /gene="gltA"
                     /locus_tag="OQS10_001039"
     CDS             26345..27628
                     /gene="gltA"
                     /locus_tag="OQS10_001039"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS2292126.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28018..28584
                     /locus_tag="OQS10_001040"
     CDS             28018..28584
                     /locus_tag="OQS10_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2292127.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28635..31093
                     /locus_tag="OQS10_001041"
                     /pseudo
     CDS             28635..31093
                     /locus_tag="OQS10_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31108..31839
                     /locus_tag="OQS10_001042"
     CDS             31108..31839
                     /locus_tag="OQS10_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2292128.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31836..32897
                     /locus_tag="OQS10_001043"
     CDS             31836..32897
                     /locus_tag="OQS10_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292129.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33049..34095
                     /locus_tag="OQS10_001044"
     CDS             33049..34095
                     /locus_tag="OQS10_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS2292130.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34092..34883)
                     /gene="nei"
                     /locus_tag="OQS10_001045"
     CDS             complement(34092..34883)
                     /gene="nei"
                     /locus_tag="OQS10_001045"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS2292131.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34919..35653)
                     /gene="pxpA"
                     /locus_tag="OQS10_001046"
     CDS             complement(34919..35653)
                     /gene="pxpA"
                     /locus_tag="OQS10_001046"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS2292132.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35643..36575)
                     /gene="pxpC"
                     /locus_tag="OQS10_001047"
     CDS             complement(35643..36575)
                     /gene="pxpC"
                     /locus_tag="OQS10_001047"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS2292133.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36569..37225)
                     /gene="pxpB"
                     /locus_tag="OQS10_001048"
     CDS             complement(36569..37225)
                     /gene="pxpB"
                     /locus_tag="OQS10_001048"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS2292134.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37248..37991)
                     /gene="ybgI"
                     /locus_tag="OQS10_001049"
     CDS             complement(37248..37991)
                     /gene="ybgI"
                     /locus_tag="OQS10_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS2292135.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38262..39743
                     /gene="dtpD"
                     /locus_tag="OQS10_001050"
     CDS             38262..39743
                     /gene="dtpD"
                     /locus_tag="OQS10_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS2292136.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9640 a        10701 c         9684 g         9763 t
ORIGIN      
        1 aattgattaa aagtcaactg ctctaccaac tgagctaacg acccatacgg gtgttacctg
       61 aaagatttct actcggggcc aatctttaag attggtgggt cgtgcaggat tcgaacctgc
      121 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccga gtggtgggtg
      181 atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta
      241 atcacccgat actgcgctgg atactacaaa ctacttccac cgaacccacc gtaattagtg
      301 gtgggtcgtg caggatgact cgcttcgctc gccctacggg ccgttgctga cgcaacgtta
      361 tcctacacgc ttaacatctg tcttcgatgc taaaactggt gggtcgtgca ggattcgaac
      421 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccactttttc
      481 gttgctttcg gtttgtttga tatcccgtgg caacggcggc atatattact gatttcagac
      541 ttgagcgcaa caaaaatttc gatgcagatc actcaactgc ttatgattcg cacgacacga
      601 ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg
      661 tgcctgttta gcgccatcag taccagggta tttactgata acctgctggt atacggcttt
      721 cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg actttaaaca tcgcatcagc
      781 agcctttggt gacttcggat agtttttcac taccgaagca aaatagtacg ccgcatcatc
      841 ttttttaccc ttgttgtagt ttaactgacc cagccaataa ttggcgtttg gcaggtaagt
      901 tgaatcaggg taatttttga tgaaattctg aaatgccacc attgcgtcat cctggcggga
      961 tttatcctgc accagcgcaa tagctgcatt gtaatccgtg tttgcatcgc cgcttttcac
     1021 cggcgcgcca gcattcgcag taccagcatc agctgtcggc gtcgttgatg ccgccgcacc
     1081 gctttgatcg ctgctggttg attgcgccgc tgcaccaccg ctgctgaggc tgtcgatctg
     1141 caacaggatc tgcttttgcc gctccacgac ctgattcagt tgatactgat tttcctgaat
     1201 ctgaccacgc agggaatcaa tatcggattg attatcagaa agttgttgct ggagttgggt
     1261 taaaagctgg ctgtgagcat tagaaatacg ctcaagttga gtgacgcggt cttcgaccga
     1321 gcctgagccg acactactga ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc
     1381 aaccagtaac gacagactca atagttgatg tctgaagtta ctgctcatgc aattctctta
     1441 gtaaaccagt accgcacgac ggtttttgga gtatgccgct tcgtcatgac ccagtactgc
     1501 aggtttttct ttaccgtaag aaacgatgga gatctggtct gcagaaacgc ctttaccctg
     1561 caggtacatc ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt attccggagt
     1621 accacgttcg tccgcgtgac cttctacggt gactttgtaa gacgggttgc tacgcaggaa
     1681 gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg atatcgtact tgtccagatc
     1741 gaagtaaacg atgttgttct gctgcagctg ttgcatttgc agacgagcct gctcttcgga
     1801 agacatgttg ccgttgccgc cgttcgcatc cataccagtg ccggcaccca gcatgccttc
     1861 gctgccgtca ttgctggcgt tcttgttgga agaacatgcc gcaattgcca taacaggcag
     1921 agcaatcatc agccctttca gcactttgtt cagttgcatt tcaatgattc ctttactatt
     1981 caattaatta ttatcacaga tacggcgacc aggcagggaa tttgacctgt ccatcagttg
     2041 ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa attcagcacg gatcccatcc
     2101 cctgagaaga gctgtagatt accatagtgc cgttaggtgc cagacttggc gtttcatcca
     2161 ggaacgtgga cgacagaact tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct
     2221 gctgcccacc gttggagctg accattacca taaatttacc gtcgctgctg acatccgcat
     2281 cctggttctg cgaaccttcc caggtaatac gttgtggcgc accgccgttg atattcactt
     2341 tataaacctg tggacgaccg gcctggtcag aagtaaatgc caggttctgg ctgtccggga
     2401 accaggtcgg ttcggtattg ttactgcgac catcagtcac ctggcggatc tgaccagaag
     2461 ccaaatccat tacgtacagg ttcaggctac cggttttcga caaggcgaat gccagtttgc
     2521 tgccgtctgg cgagaatgca ggcgcaccgt tgtgacgcgg gaatgaagcc acctgacgta
     2581 cagcgccgtt tgccagcgtt tggataacta gcgcggaacg accgctttcg aaggtcacat
     2641 aagccagttt agaaccgtct ggtgaccacg ccggagacat cagcggctgt ggtgaacggt
     2701 gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg cagttcatac gggaactgac
     2761 cgccgttggt ctgaacaacg taggcaatac gggtacggaa cgcaccttta atgccggtca
     2821 gtttttcaaa cacttcatca ctggcggtat gaccagcata gcgcagccac tgcttgttca
     2881 ctttgtacga gttctgagca agtacagtac ccggtgcgcc gccagtgtca acaagttgat
     2941 aagcaacatt gtaagaacca tccggattcg gagtgacctg accgacaact acagcgtcaa
     3001 tacccagcgc ggaccatgca gctggttgta cttcctgcgc actacccggc tgctgtggca
     3061 gacgagcgcg atctaacgga ttaaatttac cgctgttacg caagtcggca gcaacgatgc
     3121 cgccaatatc ttcaggtgcc gcaccagggc ccgcccactg gaaaggaaca acaccaatag
     3181 gacgaccgga atctacaccg ctgtcgatca caatgcggac ttcagcatgc agaactgatg
     3241 cccacagtat gagaaaacca aatgctactc gtaatgcctg cttcatcata tctcccttat
     3301 ctggaccgat ggcccacgat aatttagcag aatgttaaca aactaaaata cacaaaacta
     3361 ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcg gaacagtcaa catcgcgatt
     3421 acggtttgaa gtccaatggc gcgtttttga acacttcata tactgcctgg cttggtggtt
     3481 tcgggatctt cgcaagttta gctgctgcca acgcagcctg acaaagtgcg ggatcgccac
     3541 cttcaggttt gatatccagt aacataccat cgggtgccag ttttatgcgc agcgtacagg
     3601 ttttgcctgc ataggacgat gcgtcataga acttactttc gatagcagat ttaatctgcc
     3661 cggcatagtt attgatatcg gcccctgatg cgccattgtt tttagtatta ccactcccgg
     3721 cagacgaagc attgttccct ttcgcccctc cccccgtttt cggtgcattc ttaccagagc
     3781 ttagctcacc gaaaatatca tctgcctctg cggcagcttt tgctgcagcg gctttttcag
     3841 ctgctgcttt tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc gctgcttttg
     3901 ccgctgccgc ttttttgtct gcggcggctt tttctgccgc tgctttctta tcagctgctg
     3961 ccttatcagc agccgctttt tctgctgctt ttttgtcggc tgcagctttc tctgctgccg
     4021 ctttctttct tgcttcagct gcagcagctt ctgccgcttc cgctttcttc ttcagtgccg
     4081 cagcggctgc ctcggctttt ttctgcgctt cggctgcggc tttggcggct tctgcttctg
     4141 cttttttctt cgcgtctgcc gccgctttct tcgctgcttc ttccgcagct ttagcatccg
     4201 cttcggcctt cgctttagca tctgccgccg ctttcgctgc cgcctcttcc gcttgcttct
     4261 gctttaactc ggcctgtttt gcggcttctt cagcctgctt tttctgttcc tgagcagcta
     4321 accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc cgcttgtttc tcacgcagtt
     4381 cttcagcagc ctgctgttcc tttatcttgc gctgctcatc agaacgcttc gcgcttgatt
     4441 cctggctttg catgcgtttg tactgctcaa ctaccgcacc tgaatcaacc atgacagcgt
     4501 cgatggacga accaccgccg cctccggctg aagcttctat attctcatcg aacgaactcc
     4561 agagcagcgc cgcaaataag atgacatgca gcactgctga aataattatc gcccgcttga
     4621 gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt ccaaaaactg ttcgcctgtt
     4681 acccgctctc tttcaagcaa gggaaacgca gatgtttaga taggctgcgt cattaaacca
     4741 accgatttca cacccgcact atgtaacaag ttcagtgctt taattatttc atcgtaaggc
     4801 acatcttttg cgccaccgat cagaaagacc gttttcgggt tggccttgaa acggctggac
     4861 acttccgcca ccacctgctc tggtggtaaa cgctccaggc gatctttctc aaccaccacg
     4921 gtgtactgac caataccaga cacttcaaca atcactggcg gattatcgtt actgctcacc
     4981 gcctgtgatt cagtagcgtc tggcagatcg acctccacgc tctgggtgat gatgggcgct
     5041 gtcgccataa agatcagcaa cagcaccagc agtacgtcca gcaacggtac aatgttgatt
     5101 tcggacttga gatcgcgacg acctcgtcca cgcgctctgg ccatggctta ccccttgttg
     5161 ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg taaactcttc cataaagttg
     5221 tcgtaattca gttccagttt gtttacgcgc tggttgaggc ggttataagc cattaccgcc
     5281 ggaattgcgg cgaacagacc aattgcagtc gcaatcaacg cttctgcgat accgggcgca
     5341 accatttgca gtgttgcttg ttttaccgcc ccgagggcga taaaggcgtg catgatcccc
     5401 cagaccgtac caaacagacc aatatacggg ctgatggagc caaccgtacc gaggaacgga
     5461 atgtgcgttt ccagattttc aagttcacgg ttcatggaga tacgcatagc acgcgacgcc
     5521 ccttccacta cggcttccgg cgcatggcta ttggcacgat gcaggcgcac aaactctttg
     5581 aacccgctgt aaaatatttg ttccgaacca gtcagattat cccgtttccc ctgactctct
     5641 tgatagaggc gagagagttc gattccagac cagaatttat cttcaaacgc ttcggcttcg
     5701 cgcgccgctg cgttaagaat acgggtccgc tggataataa tggcccagga tgcgattgaa
     5761 aaaccaatca aaatcaacat gataagttta accagaaggc tagccttcag gaacaaatca
     5821 aggatattca tgtcagtcac tgcttaaact ccgcgacaat agacttggga agcgcacaag
     5881 gcttcatttt gagtgggtca acgcaaacaa ccagaacctc tgcttcattc agcaaagtat
     5941 tctcggcgtt gacaatacgt tgcgtgaaaa ccaaagaggt gccacgcatt gatgttattt
     6001 cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc gtaatattcc accgtcattt
     6061 tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg ctgactgaag tgatgatgac
     6121 gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta actggcgtgg tacaccacac
     6181 caccggcatc ggtatcttca tagtagacgc gaaccggcca tcgaaacagc gttgtattca
     6241 ctttacatcc cggtaatgca acaaaagtta gagcttttaa acgctgctac tatacgcgcg
     6301 cgattgaggt ttgggaatga gggcaagtta agggagcgta aaaatttatg ggcctggagc
     6361 ggcccataat tttttaaaga aaattctgat ttaaaagaag aagaaaatca gcccgacaat
     6421 gagaacaata tcggcaagca atgggcaaaa aatcccttgc caaagaacct tctgcggacg
     6481 aaagcccacg ccgtgaatca caccagcaca gacggcccac atcagcaata aaccatgcca
     6541 gatttccagt tcactggtct tcgcggcaaa acgagatggg tcccaaaaca tacatcctgc
     6601 taacagaagc gccatcacga aggaaagcgc ccgcaggggg cgcttgtcca ttaccgcata
     6661 caaggtcgcg ataatcttac tcatcagatg tcttcttgac cggctttgcc tgattcgacg
     6721 tgttcaagcg ccagcgcggt gattacccca aacgaacagg caagaagcgt tcccagaatc
     6781 catgcgaaat accacatttt tagctcctta cttagtacag agagtgggtg ttacgttcaa
     6841 tatcttcttt ggtgatacga ccgaacattt tccagtaaca ccaagcggtg tagagcagaa
     6901 tgatcggtac cagaaccacc gcaacccagg tcatgacgtt aagcgtcagc tggctggaag
     6961 ttgcatccca cattgtcaga cttgcgttca tcatggtgct ggacggcatc acaaacggga
     7021 acattgcgat accggctgtc aggatgatgc aggccagcgt cagggaggag aacacaaacg
     7081 cccacgcggc tttatccata cgtgcagtca ggatggtcag cagcggaaga accacaccca
     7141 gtgccggaat agcccacaga attggcgtgt tgttgaagtt aaccagccat gcgccagctt
     7201 cacgaaccac ttctttattc agtgggttag aggctgcgta atggtccatt gtcgatttca
     7261 cgacataacc atcgataccg tacatcaccc atacgccagc cagtgcgaaa cagaccagtg
     7321 tcaccagcgc agccacctga gccgttgcac gggtacgcag gtgcagttcg cccacggtac
     7381 gcatttgcag ataggttgcg ccctgagtaa tgatcatccc tacgctcacc acgcctgcca
     7441 gcaggccgaa cgggttaagc aactggaaga agttaccggt gtagtacaga cgcagatatt
     7501 catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa cgctacacca attaccagcg
     7561 gcggaacgaa actaccaatg aagatgcccc agtcccacat gttacgccag cgggtttctt
     7621 caatcttgga gcggtagtca aaaccgaccg gacggaagaa caaagacgcc agcacgagga
     7681 tcatcgccac atagaagccg gagaacgcag cggcatagac catcggccag gcagcaaaga
     7741 gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc ccagtgtggt gcaatggagt
     7801 taatcataat tcgacgctcg gtgtcgttac gaccgaggaa acgggtgagc atgcccaccc
     7861 ccatgtcgaa accgtcagtg actgcaaaac caatcagcag aacgccaacc agcagccacc
     7921 agataaaacg caatacttca taatcgatca tttgacgact cctgtcttag cgtgccggct
     7981 gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt tttcaggctg cttgggccga
     8041 ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag gaacagggta tacaggccgc
     8101 aaatcagcac cattgagaag atgagatcgc ctgcggtcag tgacgagttc gccacagctg
     8161 tcggcagcac ttcaccgata gcccacggtt ggcggccata ttcagccacg aaccagcccg
     8221 cttctacagc aatccacggc agcggaatac cgtacagcgc ggcgcgcaga agccattttt
     8281 tctcgccaat gcggttgcgg atgacactcc agaaagagag cgcgatgatt gccagaagca
     8341 ggaagccaca cgccaccatg atacggaacg caaagtacag cggcgctaca cgcgggatgg
     8401 agtctttggt tgcctgttga atctgcgctt cagtcgcatc agccacgttt ggcgtatagc
     8461 gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct attgaactgg tcacgaaccg
     8521 cctggtcggt agaaccagaa cgcagttgtt cgagcagaga gtacgccttc atcccgttac
     8581 gaatgcgttc ttcatgctgc accatcagct ctttcaggcc gataaccggg gtatccacgg
     8641 aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat cgcaaatttg ttcgtctcct
     8701 cttcctgatc aggaatgccg aacagagtaa aggcagcagg cgcaggttgc gtttcccact
     8761 cggcttcaat agcagccagt ttggttttct gcacgtcgcc catttcgtag ccggattcat
     8821 cacccagaac aataacagac agaacagcag ccataccgaa gctggcagcg atagcaaagg
     8881 agcgtttagc gaaggcgaag tcacgacctt tcagcatata ccatgcgctg ataccgagga
     8941 tgaacatcgc gccagtcaca taaccagacg ctacagtgtg aacgaatttc acctgagcaa
     9001 ccgggttaag caccagctcg gagaagctca ccatctccat acgcatagtt tcaaagttga
     9061 aatcggacgc gattgggttt tgcatccagc cgttcgcaac cagaatccac agtgcggaca
     9121 ggtttgaacc gagcgccacc agccaggtga cacacatatg ctgaacttta cccagacgat
     9181 cccaaccgaa gaagaacaga cctacaaagg tggattcgag gaagaaggcc atcagacctt
     9241 cgattgccag cggcgcaccg aagatatccc ctacatagtg ggaatagtaa gaccagttag
     9301 tcccgaactg gaactccatg gtcagaccgg tagccacacc cagagcgaag ttgataccaa
     9361 acaacttgcc ccagaacttg gtcatatctt tataaatctg tttgccggag aggacgtaga
     9421 ccgtttccat aatggccagc aggaacgcca taccgagcgt cagtggcaca aaaaggaagt
     9481 ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc gactatatct aacatcatga
     9541 ctccttgctc atcgcatgaa gactccgaga gtgaaccccg ttagaaaggg tcacacgcat
     9601 gccccaatag aaatttgttt gaacactttc atcataccat gtgaatgcac acaaaaacgg
     9661 cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat tgatcccacg tatatattac
     9721 gctgcaaaat ccttacaata aacaggtttt tgttgaagca aatttgcatt tttcgacggt
     9781 gatcaattta tagctaaatt accgcctttc agccaatttg atcgagaaca atttatctct
     9841 ttttgatgcc aatttccaag acttatacat tgataaatat caattcctac aaattatgtt
     9901 aatcatgttt caataaggta actttagtga catttatgtt taaaatgtgt gagttataag
     9961 gctgaaatta gtttcggttg gcaatttaaa gataaaaaga gacgaagcaa aaacagcacg
    10021 gttgacctta attctttttt tggtcactgg ctaatacgac gcagagtttc aggattacta
    10081 ataaaggcat aaatccaccc cgccgatatt aacatttaat taacttgtaa ttatattata
    10141 attaactcac ccagggttat tattttgtta aaaataaagc cacatatacg tggcttgaga
    10201 gtgaagataa acgcaaaatt acctgatgcg ctacgcttgc cagtgtctac atcatctctg
    10261 caatatattg aatttgcctg cttttgtaga ccgaataaag cgttcatgcc gtatccggca
    10321 tgaacaaagc gccctttgtc aacaatctgg ccgcgcatat gtggcttcgg tttgattgct
    10381 attcaggcaa gccggtaact gaacgtccgt gactggccgg gtaaaaaagg ccccgataga
    10441 tttgaacctt gattttcctc ggtggtaatg tgttcatcca tcatcgtttc tgagcaagaa
    10501 atcacctcgc gactgaaagc aacagtcgca tcacaggttg ctgattcagc cggattaaac
    10561 agccgtaaaa tcacttcttg tcggtcttca gctttcttaa gtgcgcttat caggcatccc
    10621 actgggggca ttttcaacaa actataactt tccggcacgt tgaatccggc tttgttgagc
    10681 ttcatcgcat cccatgggat tttgttgtaa cactgtactg gagtcagcca tgctcgcgcc
    10741 tgctgagcta caccagcggc ggttggcgta ccggtataac tcaataaact taggcgacaa
    10801 gaaagcagac cacgtagttg tgagtccggg actggcattt taattcccga aggccgccca
    10861 ggccttaaaa gcagatcttc tttgcccagt aagcccacgc cacgcagcaa cgtaatggca
    10921 aaggttttct tctcttcacc gatgacttca aattcacgta acccttcgct aaagacagcc
    10981 atgccgttac gcccttcctg taaggcaaca tagttgagca tattccatac cggaaccggc
    11041 gcttctttcc agccttcttg ctgccagttg ttcattgcac tgtcgttcac ggggcgcgtt
    11101 agcgaaccaa actgcgtatc tgccagaaca ctgtcagtgt taaaaggtgt agggaccagg
    11161 acacgaaggc gatgatcgtc agcctggtta tcaaggttga tatccacatc aatacgcctg
    11221 ctgttatgac taagagtgac aaccaacacc ccccctactc tgccagtgga ttgccgggcg
    11281 ctgcgttctg acaaattgag cggcactgcc atgtcatagc ggataacagc cctgctctgc
    11341 caggcttcat gaataatatc gcattgcggt ttcgcgttcg ctgcggtaat tacccactct
    11401 tcttttgcgg gtgaatagtc atattcatca ccatcatcag agctttcttc aatttgcaat
    11461 acccggtcat agcgcacacc gctgtcttta tctaccagtt gcagagaacc atcctcattg
    11521 agcgcaatct gccagaaagc attttccaga atcccttcag cgtcactttt tgccgcaatc
    11581 acgttgccag gctgattcgc ttcaatataa agcgtgcgat agcccataga agggacaatc
    11641 tggttgatct gaatatcaaa ctccataaag ggatcataat tgccataatg aacaatttgc
    11701 cgatcgatta ggccaggatc gatctcacgg gcatggcgaa taaaatacgg tacaggctga
    11761 ccgcgatcgt cttgtagatt aaactggctg gcgcgcagcc gcacagtggt gttaataact
    11821 tcttcccgcg gccagggcat caggttaaac agcacgagtt tgtcggcatc actctgtggc
    11881 atgttatcgg caattttgcg catgtagaaa cgcagcagat tatccgccat gtcttcagcc
    11941 agttcgaagc gggcgacaat ttcgcgatga actttgtcac tgcaacaaca accgatactg
    12001 tcgtgggcat gattttttaa gatctctttc cacattttct ccagcaagcc gtggtggtat
    12061 tcaaaaccca acgtccaggc cagcgttgcc agcggttcca gcagattaac aatcttattt
    12121 tcaatacgtg cgtgggcaat tttgatatcc atgcgcgtgg aaccgatggt gcgatgtacg
    12181 cgcatatatt tgccatcgat aaattcacct ttcagggtgg ccagactttc acgctgcgct
    12241 tcgatcttct caaatacctc ttcaaagcgg ctcatcacaa acttacgttg agggtagatc
    12301 tcacgtagct tatccatcac ttcgaagata ttttgctgca atggcatctg gtcatgacca
    12361 ttcggcagca aaatctcttt ggttacagac gctttttcca gcacgtcaaa ataactgtcg
    12421 aggcgtttac gtaatccgtt ttcgtcggca ggtaagtact taccgatggc gtagcccagc
    12481 ggcagcacct gcgccgtcac ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta
    12541 tcagtaccgt ggcgctccga acatccgcgc cagaacatag tgcgggtaat gccaaatcca
    12601 ttgtaaatat gcggcagttg cccggacatg ccaaaggaat ccggtaaata acctattttc
    12661 atcggctcgc caaacgcgag gcagtcacgc attccgtaca tcagattacg gacgatggat
    12721 tccgcagaaa caatcgtggt atcggtctgg gtataccagg ggccgataat caacttaccg
    12781 gcttctacct gtttcttcac gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg
    12841 aggatcgccg tttgcccgtc gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg
    12901 cacaggatct cttccatatt attgaccagc agaatgcgtg actcttcggt ggtgaaatac
    12961 cactcccgat cccaatgcat atgcggggtg atgtgaacgc gagataccgc tttcatcgtc
    13021 gtttcctttt tgtttatggc attacgccat cagtcagata gttgccatgc ttaaccgcgt
    13081 gacgccgcca gatcaggaga attgcagtcg agattgcagc ccccaccaat gccgcaccaa
    13141 accagccaat tgccgccata acaccgcccg cgccattatc atgaagtaaa aagagcgaga
    13201 aaatgccggc accgggtgtc gaaaggccga tattcatcgc accgacaata gcgcccgtta
    13261 ccatagagcc cagcacaaac gaaccaataa cccgcagcgg atcttcaatc gccatcggga
    13321 tcgccccttc ggtaatacct gccagcccta acagccaggt ggatttcccg gtctcaattt
    13381 caaactcttt aaacaggcgc ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa
    13441 ccattttgac ggaggcgaaa atggcatacg ggccgtaaac gccgttcgcc attgcgccca
    13501 ggcagaatgc ataagcggct ttgttcactg gccctccaag gtcaaaggaa cacataaaac
    13561 cgagaatggc acccagcaac agcgcgttac ttcctgacag accgttcagc caggcggtaa
    13621 gcgagttatt gatccatgcg acaggttctc ccaccacaaa cagcatcaga ctgcccgctc
    13681 ccaacgtacc gagcaccggg tagagataaa aagtcaggaa tccattgaat ttactgctaa
    13741 gacgcaagtg atttttcacc cagcgcatca agtaaccggc tatcaatccg ccaacgaccg
    13801 cgccgagaaa cccggagccg atcatgttgg cggcaagtcc agccgcaaag cctggcgcta
    13861 acgccggttt atctgccaga gaataggtgg tataggccgc gagcaccggt accatcaaaa
    13921 ttccgagcag cccgccgccc agcttgcggt acatccacag ccaggagttt tcttcattaa
    13981 acagatcttg tagcccgaag atttgcgaca gtaataccgc gaccgccagc accgtgcccc
    14041 ccgcgacaat caacggtacg gcaaaagaga ttccgctcaa cagtgcctgt ttcagctccg
    14101 ttttgacact tttcaccggt tgcgtatctt gctgtacggt acgcgtctca tcgctacgct
    14161 tgagggtaag cgcttgttgg atcaacgctt ctgcatggcg aatcggctcg gcaacaggca
    14221 ctgaaagcgc gggaatgcca ttaaaacgct cactctcctt gatggcgact tcagccgcaa
    14281 aaatacaggc ggttgcacta ttgagttgat ccgccgttaa acgcccttca atgccgttag
    14341 cgccttgttt ttcaacgtaa acatttacgc cgagtttgcg tccggctttt tccagatatt
    14401 ccgcagccat ataggtgtga gcaatacccg ccggacaggc cgttacgcag acgatagttg
    14461 gcgcgttgga aaaagaggca gaaggttgcg tgcctccctt gtcatccagc gccgagagca
    14521 actcatcagg cgtcgtcgcc gactgtatac gcgcccgaat ctcatcatcc gcaaggcgcg
    14581 tggtcagcgc tgtcagcagt tgcatatgcg tagtacccgc ttcattgggg ggaatcgcca
    14641 gcagcaccac taaatcaact gcttccgggc catcaacgcc ttcccactga agcggctcgc
    14701 tgagtgtggc gaccgcaaac gccgcttctt ttaccgcagc agttttgcca tgcggcacag
    14761 ccaacccttc acctaaggcg gtcgggccaa ggctttcacg gcgatacact tcttccagaa
    14821 attgctcagt actggaaatt ttccccagag cagcaagacg ttgagtcaac gcgtggatgg
    14881 cctcttcacg gctggtaaag cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg
    14941 tcagattcat catgatcctc acttgatcgt ttttatagtc gcgccagtgt gcccgataaa
    15001 acaataccat catgtgatca atttcacttg attgaaatta tttgtattgt atttgtatta
    15061 tttacaggat gaatttatcg ggcataatgg ctaaattcat attaatgaga tactaaaaat
    15121 gggacacaag cccttatacc ggcagattgc cgatcgcatt cgtgaacaaa ttgcgcgtgg
    15181 cgagcttaaa cctggcgatg cgttacccac tgaatcagcg ctacaaacag agtttggcgt
    15241 cagccgggtt acggtgcgtc aggctttgcg ccagctagtc gagcagcaga tcctcgaaag
    15301 cattcagggc agcgggactt acgtcaaaga agagcgggtc aattacgata tttttcagtt
    15361 aaccagtttt gatgaaaaac tgtcggaccg tcacgtcgat acacacagtg aaatcctgat
    15421 atttgaagtg attccggctg acgattttct tcagcaacag ctacaaatca ccgcgcagga
    15481 tcgtgtgtgg catgtgaagc gcgtgcgcta tcgcaagcaa aagccaatgg cgctggaaga
    15541 aacctggatg ccgcttgcgt tgttcccgga tctcacctgg caggtcatgg aaaattcgaa
    15601 atatcacttt attgaagaag tgaagaagat ggttatcgat cgtagcgaac aggaaatcat
    15661 tcctctgatg ccaaccgaag agatgagccg tctgcttaat atcagccaga caaagccgat
    15721 tcttgaaaaa gtatcgcgtg gatatttagt tgatggtcgg gtgtttgaat atagccgcaa
    15781 cgcttttaat accgatgact ataaatttac cctgatagcg cagcgaaaat catcgcgata
    15841 agcacaaaaa aggccatcat acgatggcca accatgtcga aaccgacagc ccttcgccat
    15901 tcctgtagag ggaagcggcg agggctattt cttattacag atatttattt cagaacagtt
    15961 ttcagtgctt caccgatatc cgccaggctg cgaacggttt tcacgcctgc ggcttccaga
    16021 gcagcgaatt tctcatccgc agtccctttc ccaccagcaa tgatcgcacc cgcgtggccc
    16081 atacgtttgc ctttcggcgc agtcacacca gcgatgtaac ctacaactgg cttggtaacg
    16141 tgctctttga tgtacgctgc cgcttcttct tcagcgctac cgccgatctc accgatcatc
    16201 acgatcgctt cggtctgcgg atctttttcg aacatttcga ggatgtcgat aaagttagag
    16261 cctgggatcg ggtcaccgcc gataccaaca caggtcgact gaccgaaacc gtaatccgtg
    16321 gtctgtttaa ccgcttcata ggtcagtgta ccggaacggg aaacgatacc cactttaccc
    16381 ggtttgtgaa tgtgacccgg ctggataccg attttgcatt cacccggagt gataacgccc
    16441 gggcagttcg ggccgatcat acgaacgccg gcttcgtcca gtttcacttt cacagtcagc
    16501 atatccagcg tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc tgcatcaata
    16561 gcttccagaa tggagtcttt gcagaacggt gctggtacgt agataacaga agcggtagcg
    16621 ccagtagcag caacagcttc acgcacggtg ttgaacaccg gcaggccgag gtgggtagta
    16681 ccgcctttac ctggggttac gccgccaacc attttagtgc cgtatgcaat ggcctgttct
    16741 gagtggaaag tcccctggct accagtaaag ccctggcaga taaccttggt gtttttatca
    16801 attaaaatgg acattatttc ccctccactg cggcaacaac ctgctgagct gcatccgtca
    16861 gaccttttgc tgcaataata ttcaggccgc tgtcagccag tttcttcgcg ccgagttcgg
    16921 cgttgttacc ttccagacgt accacgaccg gtacgttaac acccacttct gctaccgcgc
    16981 cgatgatacc gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg ttaaccagaa
    17041 cggctttcac tttgtcgtca gagaggatga ttttgaacgc ttcggttaca cgttctttgg
    17101 ttgcgccgcc gccaacgtca aggaagttag ccggttcgcc gccgtgcagt ttaacgatgt
    17161 ccatcgtacc catcgccaga cctgcgccgt taaccataca accgatgtta ccgtccagcg
    17221 caacgtagtt cagttcccac tgtgcagcct gtgcttcacg cggatcttcc tgcgactggt
    17281 cacgcatttc gcgcagatca ggctggcgga acagtgcgtt gccgtcagcg cccagtttgc
    17341 cgtcgaggca aatcagatcg ccctgtttgg tgatgaccag cgggttgatt tcgatcaacg
    17401 ccaggtcgcg ctccaggaaa atggtcgcca ggcccatgaa gattttggtg aactgctgaa
    17461 ccagtttacc ttccagaccc agtttgaacg ccagctcgcg tccctgatac ggcatcgggc
    17521 cagtcagcgg atcaagcgca actttgtgga tcaggtgcgg agtttcttcc gccacttttt
    17581 cgatttccac gccgccttcg gtggaggcca taaagaccac acgacgggaa ctacggtcta
    17641 caacggcacc gagatacagc tctttagcga tatcggtcgc tgcttcaacc agaatctggt
    17701 taaccggttg gccattggca tctgtttgat acgttaccag acgcttgccg agccagtttt
    17761 ctgcaaaagc acggatgtct tctttgctgt ttacaacttt cacaccgccc gctttaccgc
    17821 ggccaccagc gtgaacctga catttcacta cccacgggcc ggcaccgatt tttgaagcgg
    17881 cttcttctgc ttcgcgcgga gtagtacagg cataacccac cggtgctggt aagccatagc
    17941 gggcaaaaag ttgttttgcc tgatattcat gtaagttcat gtgttctgtc catccttcag
    18001 taatcgttat cttttaaacc gtagaccgac accctatcca cgtcggtcta cagtgcaggt
    18061 gaagcttaaa ctactacacg tccagcagca gacgcgtcgg atcttccagc aactctttga
    18121 tcgttaccag gaagcccacg gattcgcgac catcgatcag acggtgatcg taggacagcg
    18181 ccaggtacat catcggcagg atctcaacct gaccattcac cgccatcgga cgatctttga
    18241 tagcgtgcat acccagaatt gcgctctgcg gcgggttgat gatcggcgta gacatcaggg
    18301 aaccgaacac accaccgttg gtgatggtga agttaccacc ggtcagatct tcaaccgtca
    18361 gcttgccgtc acggccttta actgccagct ctttgatttt cttctcgatg tctgccatgc
    18421 cgagggtatc gacatcacgc agtaccggcg tcaccaggcc gcgcggcgtg gaaaccgcca
    18481 tgctgacgtc gaaatagttg tggtaaacca cgtcatcacc gtcgatagaa gcattcactt
    18541 ccgggtaacg tttcagggct tcaaccaccg ctttcacgta gaaggacata aagcccagac
    18601 ggatgccgtg gcgtttttca aacgcttcac cgtactgctt acgcagatcc ataatcggct
    18661 tcatgttgac ttcgttgaac gtggtcagca tggcggtgga gtttttcgct tccagcagac
    18721 gctctgccac acgcttacgc aggcgagtca tcgggacacg tttttcacta cgtgcagcca
    18781 gagccggttg cgccgccgga gcagccgctg ccggagcaga ctctttcgcc ggggctttcg
    18841 ccagatgttt ttccacatct tcacgagtca gacgaccacc cacaccggtg cctttaatgg
    18901 cgctggcgtc gagattgtgt tcagccagca ggcgacggat cgccgggctt aacgcatcgt
    18961 tgttttgctc ttccagagac gcctgctggc gttgcgccgg agtggacgct ttctcttcag
    19021 atttggcgct ggtttcttta ccggcgctgt tgccttcacg caggcgacca aggatctgac
    19081 gagacgttac cgttgtacct tcatcttcca gaaccgcatc cagaatgccg tctgctgatg
    19141 ccggtacttc cagtaccact ttgtcagttt cgatttctac cagcacttca tcacgtacga
    19201 ctgcgtcgcc gggtttttta tgccaggttg cgacggtggc atcggctacg gattcaggca
    19261 ggtcagggac cagaatatct acgctactca ttgtgtatcc tttatttatt cgacgttcag
    19321 cgcgtcatta accagatctt gttgctgttt ctggtgaacg gacatatacc ctaccgccgg
    19381 agaggcggag gccgtgcggc ctgcataacg cagagaagcc ccaaacggaa tcacttcacg
    19441 gaaatgatgc tggctgcagt accatgcgcc ctggttgagc ggctcttcct ggcaccagac
    19501 aaaatccttg acgtgagcaa actgctgcaa cacttcctgc atcgctttat gcgggaacgg
    19561 gtagagttgc tcgatacgca caatggcgac atcgtgttga ttgttcttac gacgctgttc
    19621 cagcaggtcg taataaacct taccagaaca catcactacg cgcttcacgc ccttcggatc
    19681 aagctcgtcg atttcaccga tggctggcag gaaggtgccg ttcgccagtt cttcgaggct
    19741 ggaaaccgcc agcggatgac gcagcaggga tttcggcgac atcactacca gcggacgacg
    19801 catcccgcgc agcgcctgac gacgcagcat gtggtaaacc tgtgccgggg tagacggaac
    19861 gcaaacctgc atgttttgct cagcacaaag ttgcagataa cgttccagac gcgcggagga
    19921 gtgctccggc ccctgccctt cgtaaccgtg cggcagcaac atcaccagac cacacatccg
    19981 gccccatttc tgttcgccag aagagatgaa ctggtcgata accacctgcg caccgttggc
    20041 gaagtcaccg aactgcgctt cccagatggt cagagtgcgt ggttctgcgg tggcataacc
    20101 gtattcaaac gccagcactg cttcttcaga cagtacggag tcccagacgc ggaacgcgcc
    20161 ctgcccgtta tggatgtgtt gcaacggcgt gtaagtggaa ccgttagact ggttgtggat
    20221 caccgcgtgg cggtggaaga aggtaccgcg accggagtct tcacccgaca ggcgaaccgg
    20281 aatgccttcg tcaaccagcg tggcgtaagc gaggttttcc gcaccgcccc agtcgaacag
    20341 tttctcaccg gcagccatcg cctggcgatc gccataaatc ttggcaacgc gagactgcat
    20401 ttcaactgct tccggcaccg tgctgatgcg tttcgccagc tcctgcaggc gcttcatctc
    20461 aactttgttc gggtactctt cgtcccattc gtggttgagg tacggcgacc aggtgaaaga
    20521 gtgcatgttc atcggacgcc actctgccac tacgcaatcg ccagcatcca gcgcatcgcg
    20581 gtacaggtta accatctcgg tggcatcttc cagcgtcgcc actttttcct gctccagctt
    20641 gtcagcgtag attttgcgcg gtgtcggatg ttttttgatt ttctgataca tcagcggctg
    20701 ggttgcgctc ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcaca ccaggtcgat
    20761 gaagacatca cgtttaaagg tgttacggaa atcgagcgcc agacgggtca caaaggcaac
    20821 ggcttccgga tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct taccgatatc
    20881 agtacagtac ggtgtagaac gagcatccag tggattagag gtggtgaaac caacctggtt
    20941 gttgataacg atacgtaccg taccgccaac ttcataacca cgcgctttcg acatgttcag
    21001 agtttcctga accacgccct gcccggtcac tgcggcgtca ccgtggatgg tgattggcag
    21061 cactttgttg ctgctcggct catcaagtct gtccagacgg gcacgaacag aaccgataac
    21121 taccgggctt acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt gcaccaggcc
    21181 gccatcggtc tggaagtcag acgagaagcc catgtggtat ttcacgtcac ccgtgccgag
    21241 gtgttcttta tgtttaccgg caaactcgtc gaacaagtct tgcggttttt tacccagcac
    21301 gttcaccagc acgttcagac gaccacggtg cgccatccca agaaccactt cgcgggtgcc
    21361 gctgttgcca gcgtggcgga tcatctcttt aagcatcggg attaacgcgt caccgccttc
    21421 cagcgagaag cgttttgcgc cagggaattt tgcgccgagg taacgttcaa ggccttcagc
    21481 ggcggtcagt tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg cgcgaccaga
    21541 ctcgatacgc tgttggatcc agcgtttttc ttcggtgctg gtaatgtgca tatactcggc
    21601 accaatcggg ccacagtagg tttgcttgag ggcttccagc agctcgccga gtttcatgtt
    21661 ttctttgccg ctggcaaatg aaccgacgtt gaaggtctcc tggaagtctg cttcggtcag
    21721 atcgtggaaa gacggatcca gatcggccac tttatcttgc tgccacagtc ccagcggatc
    21781 gagattcgca tgctggtgac cacggaagcg gtatgcgtta atgagctgca ggactttaac
    21841 ctgcttcaca ttggtgtcag ggtcggagat cgttgaagag taacgtgaag cgtctttcgc
    21901 caggcggcgg aaatattcac gcgtttgaga gtggaattga tccggtttga ctcccgtacc
    21961 aggtaactgc tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat cggttaagaa
    22021 gtcttcatag agctgttcta tccagctctg gtttgcgcca gagaggtaag aagagtccaa
    22081 ccaggctttc aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt tatgcttact
    22141 tcgccgtgga tactaccacg cacagtgatg tgcgtgccgg ggttcacttg cgagctcttg
    22201 tctatgtagt ggctcgcgaa ggaaccttta aaaactgtct tagtgtcagg ggcagttttt
    22261 aaagatttcc tgcatttctt tgttacgcct gatgcgctgc gcttatcagg cctacgggta
    22321 atgacttgta ggccggataa ggcgttcacg ccgcatccgg cactggttgc ctgatgcgac
    22381 gcttgcgcgt cttatcaggc ctacggttta cgcattacgt tgcaacaaca tcgacttaat
    22441 atggccgatg gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat
    22501 gctgtgacag cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc
    22561 ggtatcacgg ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa
    22621 cttatcggga ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca
    22681 ttcatacagc ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg
    22741 cggattttgt ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg
    22801 tcccatgtct accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat
    22861 cttcttgccc ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc
    22921 gttcatgttc agaccgtcgg aaccgcacac accttcacgg caggagcggc ggaacgacag
    22981 gctgggatct ttctctttca gctggataag cgcatccagc agcatcatgt cgcgaccttc
    23041 ttccgcttcc agggggtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg
    23101 ataaattgaa aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg
    23161 cgggaatgcc gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga
    23221 ctctggcaga tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa
    23281 gcggctatgt gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc
    23341 cgtttccatc aggttatcca gttccaagca ctcaacacgc tgggtgttga actcgctgga
    23401 agtgtcatcc agacgggcat ttttcagacg ctcgcggatc actttcaact gctcaagccc
    23461 tttcgccatc gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag
    23521 cgctttacga atcgccaccg gatcttcacc gttacgatta ttgttccagc ggtgcagacg
    23581 atccagagat gcttcaacat cagactcgct ggcatcgcgc aatgcgccct gttcggcgat
    23641 agactcttgc agatgcagac ctgccgcacg accaaagacc accaggtcca gcagcgagtt
    23701 gccgcccaga cggttagcgc cgtgtaccga tacacaagcg atttcaccaa cggcaaacag
    23761 ccccggaaca accacatctt cgcctttctc gttcaccgtc agcgcctgac cggtcacttt
    23821 ggtcggaata ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac
    23881 cggatcgacg tgagcaaagg tacgggaaag ctccaggata cccggcagac gggattcgag
    23941 aacttcttta cccaggtgat cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca
    24001 gccgcgacct tcacggattt caatcatgat ggaacgcgcc accacatcac ggcccgccag
    24061 gtctttggcg ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata
    24121 accgccttca ccacggcaac cttcggtgac cagtacgccc gcaccggcaa tgccggtcgg
    24181 gtggaattgc cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac
    24241 accgtcgcca gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgccccgcc
    24301 agtcgccagc acggtagcgc gggctttgaa ataaaccact tcaccggttt cgatgcacag
    24361 tgcggtacaa cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc
    24421 ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg
    24481 accggtacgg tcagccgccg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga
    24541 ctgaccgcca aacggacgtt gatagatacg accatcatca agacgcgaga acggcaggcc
    24601 catgtgttcg agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc
    24661 ctggtcaccg atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc
    24721 ttcatgggta ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg
    24781 ggtcgggaag actttagaga gcagcgcaca ggtctggccg ctctgggaaa tttgcagcgc
    24841 cgcgcgcata cctgcgccac cggcaccaat cacaactgca tcaaattctc tgactggcaa
    24901 tttcatcaca caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg
    24961 acaatcacca gttgcagcat caggcgcaat gccagcggtt taacgtagtc ggtcaacacc
    25021 tgccacatgc cgatccaggc atgaatcaag atagaaaaca gcgccagcag ggtgaacact
    25081 ttggtgaacg cagaggcgaa gaaaccgatc cagacttcat atgtcagttc gccactggtg
    25141 gcgaaaaaac cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg
    25201 aggatgaaat catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga
    25261 ctcctgcgag aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac
    25321 ccgcttcgaa tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga
    25381 cgacgtgata cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc
    25441 ccataatcgc ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc
    25501 acagcaggat gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg
    25561 ctatcgccgt gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt
    25621 tcacatttct tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg
    25681 ggcacagagg ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct
    25741 tgcgggtcag acagcgtcct ttctataact gcgcgtcatg caaaacactg cttccagatg
    25801 cgaaaacgac acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg
    25861 cggcagtata ggctgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt
    25921 tatcctgaac agtgatccag gtcacgataa caacatttat ttaattttta atcatctaat
    25981 ttgacaatca ttcaacaaag ttgttacaaa cattgccagg aaaagcatat aatgcgtaaa
    26041 agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc
    26101 aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact
    26161 tgattcgcca tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta
    26221 cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt
    26281 tgatgtgcga aggcaaattt aagttccggc agtcttacgc aataaggcgc taaggagacc
    26341 ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga
    26401 tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg
    26461 tgtgttcacc tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt
    26521 tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac
    26581 cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga
    26641 acagtatgac gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac
    26701 ccgtctgttc catgctttcc gtcgcgactc gcatccaatg gcagtcatgt gtggtattac
    26761 cggcgcgctg gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcaccgtga
    26821 aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta
    26881 ttccattggt cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct
    26941 gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc
    27001 tatggaccgt attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt
    27061 gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc
    27121 actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat
    27181 tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt
    27241 ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat
    27301 gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt
    27361 ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta
    27421 cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat
    27481 gttcaccgtc attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat
    27541 gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg
    27601 cgactttaaa agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc
    27661 cgccgttcaa atggcgggtt aatttttata tgcccaaaca caaaaaattg taaaaataaa
    27721 atccactatc ggacattcat atattctaaa aaaaatatat cggtataaat tataaacaaa
    27781 tcgatgattt ttaatcatct atcttcctct cagttacatg aataaaccac caaatatagc
    27841 gcacccctat aatttccaat actttattca tcaaacattt aattcatacc atcaattaat
    27901 tacttttaca tttaataaaa ttaataatat ttttaagaaa aactaaaaac attcgacaat
    27961 caaataagat attcttgcac cgatatactt ttttcttttc tgttaaagga ctcagttatg
    28021 tttaaaggac aaaaaacatt ggccgcactg gccgtatctc tgctgttcac agcacctgtt
    28081 tatgcggctg atgaaggatc cggtgaaatt cactttaaag gtgaagttat tgaagcaccg
    28141 tgtgaaatac atcaggatga tattgataaa gaggttgaac tcggtcaggt gaccaccagc
    28201 cacattaatc agtcacatca cagcgatgcc gttgctgtcg acctgcgctt agtcaactgt
    28261 gatctggaaa actccagcaa cggttccggt ggcaagattt ctaaagttgc agtcaccttt
    28321 gatagctcag cgaaaaccac cggcgcagat ccgattctca acaacaccag cacgggtgaa
    28381 gccaccggcg tcggcgtacg tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc
    28441 gctactccag atatcgacct ggctccgacc tccagcgaac aaacgctgaa tttctttgcc
    28501 tggatggaac aaattgatca ggctacacct gtaacgccag gcgcagttac ggcgaatgca
    28561 acctacgtgc tggattataa ataagtatta ctttggcggc gctatcgccg cctttcataa
    28621 aatatttatc aggtatggac accgtgaata tttatcgact ctcttttgta tcctgcctgg
    28681 tcgtggcgat gccttgcgca ttggcggtcg aattcaacct taatgttctc gataaatcga
    28741 tgcgcgaccg cattgatatt tcattattaa aggaaaaagg agtcattgct cccggtgagt
    28801 attttgttag cgttgcggta aataataacc aaatcagtaa cgggcaaaag attaactggc
    28861 acaaaaatga cgataaaacc attccgtgca tcaatgattt actggtcgat aaatttggct
    28921 taaaacctga agtccgtcag tcgttaccat tgataaatca gtgcgtcgat tttagctccc
    28981 gacctgaaat gctcttcaat ttcgatcaag ccaatcagca actgaatatt agtattccgc
    29041 aagcctggct ggcgtggcac tcagaaaact ggaccccacc ctccacatgg aaagaaggtg
    29101 tcgccggtat cctgatggat tacaacttgt ttgccagcag ctaccgccca caggacggca
    29161 gcagcagcac taacctgaac gcctacggta ccaccggaat taacgccggg gcatggcgct
    29221 tacgtagtga ttatcagttg aatcagactg atagcgatga taaccatgaa cagtcaggcg
    29281 gaatatcgcg cacctatctt tttcgtccat taccgcaatt aggctctaaa ttaaccctcg
    29341 gcgaaacgga ttttagttcc aatattttcg acggtttttc ttataccggc gcggcactgg
    29401 caagtgacga gcgaatgttg ccatgggaac tacgcggcta cgccccacaa attagcggta
    29461 ttgcacagac caatgccacg gtgacgatca gtcaatcagg ccgcgtcatt taccagaaaa
    29521 aagtcccacc aggcccattt atcattgacg accttaatca gtctgttcag ggcacactgg
    29581 atgtcaaagt gacggaagaa gatggtcggg tgaacaattt ccaggtttcg gcagcatcga
    29641 cgcccttcct gactcgtcag ggacaggttc gctataaact ggccgcgggt cagccacggc
    29701 cctccatgtc acatcaaact gaaaatgaaa ccttttttag caatgaagtt tcctggggga
    29761 tgctgtcaaa cacctcgctg tacggcggcc tgctgctttc tggtgatgac taccattctg
    29821 ccgcaatggg tattgggcaa aatatgctgt ggcttggtgc gctgtcgttt gatgtcacgt
    29881 gggccagtag ccattttgat actcagcagg acgagcgggg cttaagctac cgttttaatt
    29941 acagcaaaca agtggatgcc actaacagca cgatttccct cgccgcttat cgtttctccg
    30001 atcgtcattt tcacagctac gccaactatc tggatcacaa atacaacgac agcgatgcgc
    30061 aggacgaaaa acagacgatc agcttattgt gggccaaccg attaccccac taaacctcaa
    30121 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
    30181 catcacggca ggttttaatg ttgatattgg tgactggaga gatatctcta tttcgacgtc
    30241 attcaataca acccattacg aagataaaga tcacgacaac cagatttacc tgtcgatttc
    30301 gctccccttc ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac
    30361 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
    30421 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
    30481 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
    30541 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
    30601 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
    30661 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
    30721 ttaccagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc
    30781 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
    30841 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
    30901 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
    30961 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
    31021 ccagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
    31081 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
    31141 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
    31201 aacgctaatg ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac
    31261 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
    31321 ctgccgccga tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa
    31381 gcctcgacca caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
    31441 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
    31501 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
    31561 ctgcagttac aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
    31621 tatctgacca ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc
    31681 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaaattatag cggcagccag
    31741 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
    31801 ggacaatgcc atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
    31861 tcgtcatctg tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
    31921 gttcatccgg tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca
    31981 atgccaaact cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg
    32041 acaacaatac caacggcaat tttgaaagtg agcatgttta tgcctgggta aacccctacc
    32101 cgggggtgca ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg
    32161 ccaaccaggg aaaaagccgc atcgacacta accagtgtat tgacagtaag aacattgaca
    32221 tttatacccc tgagcagatt atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc
    32281 ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac
    32341 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
    32401 acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg
    32461 gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac
    32521 aggtgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa
    32581 cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca
    32641 gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
    32701 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
    32761 ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac
    32821 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
    32881 aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
    32941 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc
    33001 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
    33061 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
    33121 ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga
    33181 attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg
    33241 attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg
    33301 cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt attggtgcgc
    33361 tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg cgcagcggca
    33421 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggttgcatt tatgcaatat
    33481 ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggtgat
    33541 aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg
    33601 cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg tcttccttcc
    33661 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
    33721 atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt
    33781 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
    33841 tcgatttttg ctctgctggc tatttgtgcg ggtatggcgt gggggctgac ccggtttatg
    33901 cacattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg
    33961 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
    34021 ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac ctatgcgccg
    34081 aagcggtcgg cctagtgctg gcagccaggg caccagtaaa acgggcgaga tgacagcgtg
    34141 gttttctcaa tgatgctgcc acagcgttcg cacggttcgc catcacgatg aaaaacctta
    34201 aagcgaaaca gcgccccatg atgcttattc tcatccacca gccccctcgt agcgtaggaa
    34261 aatcgaggaa tatccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct
    34321 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
    34381 ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg gttacgaaag
    34441 cgcggtgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
    34501 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa catctcaata
    34561 tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact
    34621 cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta
    34681 tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc
    34741 acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca
    34801 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
    34861 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aatcggggtt
    34921 atgctgcgac aacaatctcc ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca
    34981 gtgcgtgctc gccgtcgcca tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc
    35041 cggtgatact tttgactctg ccgtgttgca ccatctctag cgtttgcgcc aacgcctgtt
    35101 cttcgttttc aatcaacgcg cctcgctgac ttcgcggcac cagcgagccg tcagcctgat
    35161 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
    35221 tcagctcgct tcctgccagc ccaacgagaa tcaatgccgg atcgcaagcg tataccgctc
    35281 tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg
    35341 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
    35401 gatacagcgt ctgggcgtaa acggtttctg gcggcagctg catggcgctg cgaccaaaat
    35461 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
    35521 gcacgcaagc ctgcatgatt tgcgcatcgc ctgcatgaaa tccacaggca atattggcag
    35581 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
    35641 ggtcaatttt cattgtgcag ccgccacact aattgttcga aataacgttg ctgatcttgc
    35701 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
    35761 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgcgg gtaaccatcg
    35821 gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgtggcac ctgcaccacg
    35881 cccggtaaca aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc
    35941 tgtaagcgat agcccatgcg gttactttgc gagctaagct gccagggcga acgccagaat
    36001 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
    36061 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca
    36121 atcgggagtc ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccactttg
    36181 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
    36241 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
    36301 aatcgccagc cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcacccgtc
    36361 agagcaaacc acccatcagt ttcgaactca accgttaact gaccgagcgt gatctccagc
    36421 gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgtaacgc aggcatatcc
    36481 agtgcgccgc agtggctgat tcccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
    36541 gtggtataca tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc
    36601 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
    36661 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
    36721 gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg
    36781 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
    36841 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctaaacccgc
    36901 aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
    36961 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
    37021 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata ttgttcatgc
    37081 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
    37141 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
    37201 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
    37261 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
    37321 cgaataccac cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa cccctgctcg
    37381 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
    37441 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc
    37501 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
    37561 gaggctagtt ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
    37621 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
    37681 ggatgtacgt caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt
    37741 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
    37801 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
    37861 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttctacctg caaaccattc
    37921 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
    37981 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    38041 tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    38101 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    38161 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    38221 cgcaactatt acgtgacgtt gcagtcaatt gtggaattat tatgaataaa cacgcatcac
    38281 aaccgcgcac tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    38341 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    38401 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    38461 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg
    38521 cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    38581 tggcgattat cgtttgtggc tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg
    38641 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg
    38701 gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct
    38761 gggcgatggg ctttggcctg gcggcggtgg gtatgatcgc gggtctggtc attttcttat
    38821 gtggcaatcg tcatttcaca catacccgcg gcgttaataa aaaagtactg cgtgcaacaa
    38881 actttctcct gcctaactgg ggatggctgc tggttctgct ggtggcaacg cctgcgctga
    38941 ttaccgtact gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    39001 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cgtaaggagc
    39061 tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg
    39121 gcggcagctc aattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    39181 ccgttccgac tgctatgttc cagtcgatta atgccttcgc agttatgcta tgcggtgtgt
    39241 tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa
    39301 aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc
    39361 gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc ctcgcggtga
    39421 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    39481 ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga
    39541 actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    39601 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    39661 tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    39721 gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac gcttgacgcg
    39781 tcttatca
//
LOCUS       DAJVXP010000020        39660 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVXP010000020 DAJVXP010000000
VERSION     DAJVXP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.020"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..258)
                     /locus_tag="OQS10_001051"
     CDS             complement(<1..258)
                     /locus_tag="OQS10_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292137.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTP"
     gene            complement(418..630)
                     /gene="rpmE"
                     /locus_tag="OQS10_001052"
     CDS             complement(418..630)
                     /gene="rpmE"
                     /locus_tag="OQS10_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS2292138.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            833..3031
                     /gene="priA"
                     /locus_tag="OQS10_001053"
     CDS             833..3031
                     /gene="priA"
                     /locus_tag="OQS10_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS2292139.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3187..4212
                     /gene="cytR"
                     /locus_tag="OQS10_001054"
     CDS             3187..4212
                     /gene="cytR"
                     /locus_tag="OQS10_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS2292140.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4304..5263
                     /gene="ftsN"
                     /locus_tag="OQS10_001055"
     CDS             4304..5263
                     /gene="ftsN"
                     /locus_tag="OQS10_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS2292141.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5356..5886
                     /gene="hslV"
                     /locus_tag="OQS10_001056"
     CDS             5356..5886
                     /gene="hslV"
                     /locus_tag="OQS10_001056"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS2292142.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5896..7227
                     /gene="hslU"
                     /locus_tag="OQS10_001057"
     CDS             5896..7227
                     /gene="hslU"
                     /locus_tag="OQS10_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS2292143.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7294..8220
                     /gene="menA"
                     /locus_tag="OQS10_001058"
     CDS             7294..8220
                     /gene="menA"
                     /locus_tag="OQS10_001058"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS2292144.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8313..8798
                     /gene="rraA"
                     /locus_tag="OQS10_001059"
     CDS             8313..8798
                     /gene="rraA"
                     /locus_tag="OQS10_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS2292145.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8883..9128)
                     /gene="zapB"
                     /locus_tag="OQS10_001060"
     CDS             complement(8883..9128)
                     /gene="zapB"
                     /locus_tag="OQS10_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS2292146.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9554..10399
                     /gene="glpF"
                     /locus_tag="OQS10_001061"
     CDS             9554..10399
                     /gene="glpF"
                     /locus_tag="OQS10_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS2292147.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10422..11933
                     /gene="glpK"
                     /locus_tag="OQS10_001062"
     CDS             10422..11933
                     /gene="glpK"
                     /locus_tag="OQS10_001062"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS2292148.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12063..13073
                     /gene="glpX"
                     /locus_tag="OQS10_001063"
     CDS             12063..13073
                     /gene="glpX"
                     /locus_tag="OQS10_001063"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS2292149.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13170..13916
                     /gene="fpr"
                     /locus_tag="OQS10_001064"
     CDS             13170..13916
                     /gene="fpr"
                     /locus_tag="OQS10_001064"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS2292150.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHATRYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13921..14349)
                     /gene="uspD"
                     /locus_tag="OQS10_001065"
     CDS             complement(13921..14349)
                     /gene="uspD"
                     /locus_tag="OQS10_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS2292151.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14376..14675)
                     /locus_tag="OQS10_001066"
     CDS             complement(14376..14675)
                     /locus_tag="OQS10_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS2292152.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14887..15327)
                     /locus_tag="OQS10_001067"
     CDS             complement(14887..15327)
                     /locus_tag="OQS10_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS2292153.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15428..16027
                     /locus_tag="OQS10_001068"
     CDS             15428..16027
                     /locus_tag="OQS10_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS2292154.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16135..16902
                     /gene="tpiA"
                     /locus_tag="OQS10_001069"
     CDS             16135..16902
                     /gene="tpiA"
                     /locus_tag="OQS10_001069"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS2292155.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16957..17712)
                     /gene="cdh"
                     /locus_tag="OQS10_001070"
     CDS             complement(16957..17712)
                     /gene="cdh"
                     /locus_tag="OQS10_001070"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS2292156.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17819..18808)
                     /gene="sbp"
                     /locus_tag="OQS10_001071"
     CDS             complement(17819..18808)
                     /gene="sbp"
                     /locus_tag="OQS10_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS2292157.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19128..20090)
                     /gene="pfkA"
                     /locus_tag="OQS10_001072"
     CDS             complement(19128..20090)
                     /gene="pfkA"
                     /locus_tag="OQS10_001072"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS2292158.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20271..21173)
                     /gene="fieF"
                     /locus_tag="OQS10_001073"
     CDS             complement(20271..21173)
                     /gene="fieF"
                     /locus_tag="OQS10_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS2292159.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21321..21821)
                     /gene="cpxP"
                     /locus_tag="OQS10_001074"
     CDS             complement(21321..21821)
                     /gene="cpxP"
                     /locus_tag="OQS10_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS2292160.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21971..22669
                     /gene="cpxR"
                     /locus_tag="OQS10_001075"
     CDS             21971..22669
                     /gene="cpxR"
                     /locus_tag="OQS10_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS2292161.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22666..24039
                     /gene="cpxA"
                     /locus_tag="OQS10_001076"
     CDS             22666..24039
                     /gene="cpxA"
                     /locus_tag="OQS10_001076"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS2292162.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24145..24819)
                     /gene="yiiM"
                     /locus_tag="OQS10_001077"
     CDS             complement(24145..24819)
                     /gene="yiiM"
                     /locus_tag="OQS10_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS2292163.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24968..25951)
                     /gene="kdgT"
                     /locus_tag="OQS10_001078"
     CDS             complement(24968..25951)
                     /gene="kdgT"
                     /locus_tag="OQS10_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS2292164.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26211..26831)
                     /gene="sodA"
                     /locus_tag="OQS10_001079"
     CDS             complement(26211..26831)
                     /gene="sodA"
                     /locus_tag="OQS10_001079"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS2292165.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27116..28150
                     /gene="rhaT"
                     /locus_tag="OQS10_001080"
     CDS             27116..28150
                     /gene="rhaT"
                     /locus_tag="OQS10_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS2292166.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28147..28995)
                     /gene="rhaR"
                     /locus_tag="OQS10_001081"
                     /pseudo
     CDS             complement(28147..28995)
                     /gene="rhaR"
                     /locus_tag="OQS10_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(29069..29905)
                     /gene="rhaS"
                     /locus_tag="OQS10_001082"
     CDS             complement(29069..29905)
                     /gene="rhaS"
                     /locus_tag="OQS10_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS2292167.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30193..31662
                     /gene="rhaB"
                     /locus_tag="OQS10_001083"
     CDS             30193..31662
                     /gene="rhaB"
                     /locus_tag="OQS10_001083"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS2292168.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31659..32918
                     /gene="rhaA"
                     /locus_tag="OQS10_001084"
     CDS             31659..32918
                     /gene="rhaA"
                     /locus_tag="OQS10_001084"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS2292169.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33160..33984
                     /gene="rhaD"
                     /locus_tag="OQS10_001085"
     CDS             33160..33984
                     /gene="rhaD"
                     /locus_tag="OQS10_001085"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS2292170.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33994..34308
                     /gene="rhaM"
                     /locus_tag="OQS10_001086"
     CDS             33994..34308
                     /gene="rhaM"
                     /locus_tag="OQS10_001086"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS2292171.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34609..35055
                     /locus_tag="OQS10_001087"
     CDS             34609..35055
                     /locus_tag="OQS10_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS2292172.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            35066..36517
                     /locus_tag="OQS10_001088"
     CDS             35066..36517
                     /locus_tag="OQS10_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS2292173.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36507..37577
                     /locus_tag="OQS10_001089"
     CDS             36507..37577
                     /locus_tag="OQS10_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS2292174.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37577..39325
                     /locus_tag="OQS10_001090"
     CDS             37577..39325
                     /locus_tag="OQS10_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS2292175.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39374..>39660)
                     /locus_tag="OQS10_001091"
     CDS             complement(39374..>39660)
                     /locus_tag="OQS10_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCS2292176.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9630 a        10299 c        10315 g         9416 t
ORIGIN      
        1 gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca gcggggcggg
       61 cagggcgatg tcggtttcac cgggaaggac cttcgcaccg agcagcggat tgaccggatg
      121 gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg caccgatgcg
      181 caccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc cctgacgcgc
      241 cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag aagcctagcg
      301 ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag aatttataaa
      361 aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta
      421 tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac cggtagcaac
      481 atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga ggttcaggtc
      541 atgaccaacg gtggagcgga ttttcattac gttaccgcaa gagcagctag cagtaatttc
      601 ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg ccgcgtcgct
      661 cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa tacgatcatt
      721 tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt atgcaaactg atccgcactc
      781 ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg ctatgcccgt
      841 tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctgc tgccagaagg
      901 catggcggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat
      961 cggggttgtg gtatcagtta gcgatgtcag tgagctgccg cttaacgagc tgaaagcggt
     1021 agttgaagtg ctggacgttg agccggtatt tactcactcc gtctggcgat tactgctctg
     1081 ggcggcagat tactatcatc atccgattgg cgatgtgctg tttcatgcct tgccgatttt
     1141 actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg ccactgaaca
     1201 aggccaggcg gtggatctga acagcctgaa acgctcaccc aagcaacaac aggcgctggc
     1261 ggcgttacgg caagggaaaa tctggcgcga ccaggttgcc acgctcgaat ttaatgatgc
     1321 cgcgttgcag acgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt
     1381 tagcgactgg cgaacgaact atgccgtttc tggtgagcgg ttgcgattga ataccgaaca
     1441 ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact ttttctgcct ggctgctggc
     1501 gggcgttacc ggttccggta aaacggaggt ttatctcagt gtactggaaa acgtgctcgc
     1561 tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc
     1621 ccgttttcgt gaacggttta atgcgcccgt ggaagttctg cattccggtc tgaacgacag
     1681 cgagcgtctt tcggcgtggc tgaaagcgaa aaatggcgag gcggcgattg tgatcggcac
     1741 ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg acgaagagca
     1801 cgacagttcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc tggcggtgta
     1861 tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc gcaacgcccg cgctggaaac
     1921 gttgtgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacgcgcc gggcggggaa
     1981 tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt cagaaggtgc aggcaggtct
     2041 ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccaacaatc aggttatcct
     2101 ctttcttaac cgccgtggct ttgcgcctgc gctactgtgc cacgactgcg gctggattgc
     2161 cgaatgccca cgttgcgatc actactacac gctgcatcag gcgcaacagc atttgcgctg
     2221 tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg gttccacgca
     2281 cctggtaccc gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc cgctgttccc
     2341 tgatgtgccc atttctcgta tcgaccgcga taccaccagc cgcaaagggg cgctggaaca
     2401 gcaactggcg gaagtacatc gcggcggtgc gaggattttg atcggcacac aaatgctggc
     2461 gaaaggtcac cactttccgg atgtgacgct ggtggcgtta ctggatgtgg acggcgcgct
     2521 gttttctgcc gattttcgct cggcagagcg tttcgctcag ctttacaccc aggtcgccgg
     2581 tcgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcacc atccggaaca
     2641 tcctctgttg caaacgttgc tctataaagg ctacgacgcc tttgccgaac aggcgctggc
     2701 tgagcgacgc atgatgcagc tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga
     2761 tcataacaat cagcatgcgc cgctgttcct gcaacaactg cgtaatctga tcctctccag
     2821 cccgctggca gacgacaaat tgtgggttct cggtccggtt ccggcactgg cacctaaacg
     2881 tggcggtcgc tggcgctggc agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca
     2941 catcattaac ggtacgctgg cactcatcaa tacaataccg gattcccgta aggtgaaatg
     3001 ggtgctggat gttgatccga ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa
     3061 attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct
     3121 aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc caggcgagga
     3181 gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt tgccctcaag
     3241 gcaaaagtct ctacagcgac tgtctcccga gcattaatga atcccgataa agtctcccag
     3301 gccacccgta atcgggttga aaaagcggcc cgggaagtgg gttatttacc gcagcctatg
     3361 gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc ggatatctgc
     3421 gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa tcacggatat
     3481 ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg
     3541 atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc
     3601 agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt cgcaccggaa
     3661 ctggagctac ctacagttca tatcgacaat ctgaccgccg catttgatgc agtaaattat
     3721 ctatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga gatgccgctg
     3781 tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat tatggttgat
     3841 ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag
     3901 ctgcttgatc ttccacaacc gcctactgct gtcttctgcc atagcgatgt gatggcgctc
     3961 ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct ttccataatc
     4021 ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac catcgcgcag
     4081 ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa
     4141 cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg atcaacacgc
     4201 gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc gccgcttaag
     4261 taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac aacgagatta
     4321 tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca
     4381 acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg tgcttgtgac
     4441 ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg agacgctgca
     4501 aagccagaaa gtgaccggta acggactacc accaaaacca gaagaacgct ggcgctacat
     4561 taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg
     4621 tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg aacaaatgca
     4681 ggctgatatg cgccagcagc caacgcagct ggttgaagtt ccgtggaatg aacagacgcc
     4741 ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag cggagcagca
     4801 acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga cgcgtacgtc
     4861 gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc
     4921 gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc
     4981 tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg
     5041 ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga cggtacgtgc
     5101 tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc aacaatggct ggaatcgtgt
     5161 ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc ggttgaagat
     5221 ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc
     5281 ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct gtattcgtaa
     5341 ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca tgtggtcatc
     5401 gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc
     5461 cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt
     5521 acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct ggtcaaagcc
     5581 gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc tgcgcaaact tgaagcactg
     5641 ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag
     5701 ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc tgcggcgcgc
     5761 gcgctgttag aaaacactga acttagcgcc cgtgaaattg ctgaaaaggc gttggatatt
     5821 gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt aagctacaaa
     5881 gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga actggataag
     5941 cacatcatcg gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg
     6001 cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa tatcctgatg
     6061 atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa gctggcgaat
     6121 gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctatgt cggtaaggaa
     6181 gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg cgtccaggct
     6241 atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct cgacgtgctg
     6301 atcccacctg ctaaaaacaa ctggggacag accgaacagc agcaggaacc gtccgctgct
     6361 cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga aatcgagatc
     6421 gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat ggaagagatg
     6481 accagccagc tgcagtccat gttccagaac ctgggcggcc agaagcaaaa agcgcgtaag
     6541 ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa actggtgaac
     6601 ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat cgtgtttatc
     6661 gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa
     6721 ggcgttcagc gtgacctgct gccgctggta gaaggttgca ccgtttccac caaacacggg
     6781 atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat tgcgaaaccg
     6841 tctgacctga tcccggaact gcaaggtcgg ctgccaatcc gcgttgaact gcaggcactg
     6901 accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac cgtgcagtac
     6961 aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg tattaagcgc
     7021 atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta
     7081 cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga tttaagcggt
     7141 caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt ggtggcagat
     7201 gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat tgtttgatgg
     7261 ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat tagccgaact
     7321 caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc tgcaattatc
     7381 gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc cgctggtcgc cctgctggta
     7441 ctaattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta cggcgatgcg
     7501 gtaaaaggca gcgataaacc tgaccgcatt gggccgctac gcggcatgca aaaaggggtc
     7561 attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat ctgtctctcc
     7621 gggctggcac tggttgcagt ggcatgccat acgctggccg attttgtcgg tttcctgatt
     7681 cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg tccttatggt
     7741 tatatcggtc tgggtgatat ttccgtactg gttttctttg gctggttgag cgtcatgggg
     7801 agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc gaccgcgtgt
     7861 ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa tagcgaccgc
     7921 gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat
     7981 gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat
     8041 agcctgtggg gctggctgtt cctgctggcg gcaccattac tggtgaagca agcccgttat
     8101 gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc tggaacgtac tgtcaaggga
     8161 gcgttactga ctaacctgct gtttgtttta gggatattcc taagccagtg ggcagcataa
     8221 ctgacaaata tcaattaaca attgatgatt ttgccaacag cctacatagc gcgatatact
     8281 gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc gagctttgtg
     8341 acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg
     8401 cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg ttgctgtacg
     8461 atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc
     8521 gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc
     8581 tggttattta cggcgcggtg cgtcaggtag atgacctaga agagttggat atcggcatcc
     8641 aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc
     8701 gcgtcaattt tggcggtgta accttcttct ccggcgacca tctttatgcc gacaataccg
     8761 ggattattct ttcagaagat ccgctggata ttgaatgata agaaaggcac cgcaaggtgc
     8821 cttttttctg cgttacctgt tggcctacac agtaaagaaa ttacgcgaaa gatgaagcgt
     8881 aatcagacct cttccatgcg acccagcagg gcctgcagac gttcctgcca gccgttctgc
     8941 tgttctttca gatggttgtt ctcacgctcc agctcttcgc gctgatgctg ggcattttga
     9001 acttcctgcg acagtgagtt gtttttttct ttcagctctt cgatttccat ctgcaacaga
     9061 gtgatggtat caatcgcctg ctgtactttt gcttccagtt tctcaaacac ttctaatgac
     9121 attgtcatac ctctcctgaa ttgcaaggcg ttgatggata aaaaatcctc gtcccgatta
     9181 ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta tgaagccgcg
     9241 ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt catccttctc
     9301 ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg aatgatctaa
     9361 caaacatgca tcatgtacaa tcagatggaa taaattgcgc gataacgctc attttatgac
     9421 gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga taagtttaca
     9481 gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa cattaactct
     9541 tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg ctgaattcct
     9601 cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt gcagcactaa aagtcgctgg
     9661 tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg caatggccat
     9721 ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca ttgcattgtg
     9781 gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac aagttgccgg
     9841 cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt tcgacttcga
     9901 gcagactcat cacattgttc gcggcagtgt tgaaagtgtt gatctggcag gcactttctc
     9961 tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga tggtgattac
    10021 cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg taccacgcgg
    10081 ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat ctatgggccc
    10141 attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct ttgcctggct
    10201 ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact tcctggtgcc
    10261 gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca aactgattgg
    10321 tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gagaccacaa ctccttcaga
    10381 acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa aaaaaatata
    10441 tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat cacgatgcca
    10501 atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca ggctgggtag
    10561 aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa gtgctggcga
    10621 aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag cgtgaaacca
    10681 ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc tggcagtgcc
    10741 gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat tatatccgca
    10801 gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag tggatcctcg
    10861 accatgtgga aggctctcgc gagcgtgcgc gtcgtggtga attgctgttt ggtacggttg
    10921 atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat tacaccaacg
    10981 cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa atgctggaag
    11041 tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa gtgtacggtc
    11101 agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc gccggtgacc
    11161 agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag aacacctatg
    11221 gcactggctg ctttatgctg atgaacaccg gcgagaaagc ggtgaaatca gaaaacggcc
    11281 tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgctg gaaggtgcgg
    11341 tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg attaacgacg
    11401 cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg tatgtggttc
    11461 cggcctttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg gcgattttcg
    11521 gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag tctattgctt
    11581 atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt ctgcacgccc
    11641 tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag tccgatattc
    11701 tcggcacccg cgttgagcgc ccggaagttc gcgaagtgac tgcattgggc gcggcctatc
    11761 ttgctggtct ggcggtcggt ttctggcaga acctcgacga gctgcaagag aaagcggtga
    11821 ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt tacgcaggct
    11881 ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgtaatg taaatgccgg
    11941 atgaagcgtt tatgccgcat ccggtagtcc cgaaacgtgc gggggcaacc ccgcacacat
    12001 caataatccc tcccttcccc tgtgctacac ttcgcgccat tccttactgc ttagagtttg
    12061 ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg gcgctggctg
    12121 gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg gtaaacgcca
    12181 tgcgtattat gctcaaccag gtcaatattg acggcaccat cgtcattggt gaaggtgaaa
    12241 tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc ggcgacgcgg
    12301 tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc caggctaacg
    12361 cgctggcggt gctggcagta ggcgataaag gctgcttcct caatgcgccg gatatgtata
    12421 tggagaagct gatcgtcgga ccgggagcca aaggcaccat tgatttgaac ctgccgctgg
    12481 cggataacct gcgcaatgtt gcggcggcgc tcggcaaacc gttgagcgaa ctgacggtaa
    12541 caattctggc taaaccacgc cacgatgccg ttatcgctga aatgcaacaa ctcggcgtac
    12601 gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt atgccagaca
    12661 gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt tctgcggcgg
    12721 tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat gacgtcaaag
    12781 gcgacaacga agagaatcgt cgcattggcg agcaggagct ggcacgctgc aaagcgatgg
    12841 gcatcgaagc cggtaaagta ttgcgcctgg gcgatatggc gcgcagcgat aacgtcatct
    12901 tctctgccac cggtattacc aaaggcgatc tgctggaagg cattagccgc aaaggcaata
    12961 tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc cgcattcagt
    13021 ctatccacta tctggatcgc aaggacccgg aaatgcaggt gcacatcctc tgattgattt
    13081 gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag aacgaagata
    13141 aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc aaagtcacta
    13201 aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc gtgcttccgt
    13261 ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc gtccagcgcg
    13321 cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg gtcaccgtcc
    13381 ccgatggcaa attaagccca cgactggcgg cactgaaacc aggcgatgaa gtgcaggtgg
    13441 ttagcgaagc agctggcttc tttgttctgg aggaagtacc agattgcgaa acgctatgga
    13501 tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta ggcaaagatt
    13561 tagatcgctt caaaaatctg gtcctggttc acgccacacg ttatgccgcc gacttaagct
    13621 atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc attcagacgg
    13681 tggtcagtcg ggaaacggca gcggggtcgc tcaccggacg gataccggca ttaattgaaa
    13741 gtggggaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc catgtgatgc
    13801 tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag acccggcaga
    13861 tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac tggtaagcgg
    13921 ttacttatcg ataaacggca cgatgagcaa atccgcactc atcttattga tcatcccgcg
    13981 atatgccggc atcaaacggt tgataaatga gtggtgatga ccacagacaa ggaggtcgca
    14041 ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt caatacgcag
    14101 ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat ccgacttatt
    14161 cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat acaactcgct
    14221 taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg cgagctccag
    14281 ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc caatgtgttt
    14341 ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg cagtgataac
    14401 tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag ctcaacttgc
    14461 ccctccagat ccttaatctt ataggccacg ctggcccctt catctgcaga ggatgcagcg
    14521 gcggcaagct ctttcagttt ggtcataaaa gactctgctt cttcgcgggt ggcaaaaaag
    14581 cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc aatcgcacat
    14641 cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat attcgcctct
    14701 ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa gtaatgatct
    14761 tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg attttccggg
    14821 tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta agagatatat
    14881 taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctgag tatctttacc
    14941 atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca tcatcaccag
    15001 aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta aaatcgccca
    15061 gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc cggaacgccc
    15121 gcgatcatgc aagcgcttaa cagttactgc cgctgttggc caaagcaagc acacaaggca
    15181 aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa gcaccagcat
    15241 gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac gccctttaaa
    15301 tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt ttccaggatt
    15361 ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat aacaaaaggt
    15421 actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat taaccgttac
    15481 aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc tgctggctgg
    15541 agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca gccagaatcc
    15601 tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag ccaatctcac
    15661 tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg agcgcggtac
    15721 cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag agcaaaaagc
    15781 agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca ccccattaat
    15841 gaccaaaaca caaagccaga aaaaactgca gtcgctacta acggcgggga aaaacaaacg
    15901 ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaaca gcgaaaaggg
    15961 gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg aaggtttgaa
    16021 taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc acagacctgc
    16081 tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat taaaatgcga
    16141 catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt tcacgagctg
    16201 gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc aatcgcacca
    16261 ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat catgctgggt
    16321 gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc tgccgctatg
    16381 ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg tacttaccac
    16441 aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca gggcctgact
    16501 ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac tgaagaagtt
    16561 tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt cgaaggtgcg
    16621 gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac tccggctcag
    16681 gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc taacatcgct
    16741 gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc agaactgttt
    16801 gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc tgacgctttc
    16861 gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca ggtgccggat
    16921 ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct cacactgatg
    16981 atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt gtgtcgccag
    17041 caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc ccatatgttc
    17101 ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta ccagttcgct
    17161 ttccgttacc cgacgcgcca ggtattcatg cccacgcaaa ccacctggca gtggcaacca
    17221 gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag gacgaataca
    17281 agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga tcgccaaaga
    17341 aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac gcgcctgcca
    17401 ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt cagtaccgtt
    17461 aatacgatac gttggcatca acagatattg cagtggacca ttaagatctt ttaaaaccac
    17521 gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt gctgctgatt
    17581 gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac cggttaattt
    17641 ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa aaagaagacc
    17701 cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac gcaaaatcgt
    17761 gacaaataaa aaacccggtc gcgagaaacc accgggtttg ataattatcc tggggaaatc
    17821 agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt tgcgctttcg
    17881 tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac gcattttcgt
    17941 actttttcgc tacttcagcg tcgcgcggac ggtagtagtt tttcgcggca atttcctgac
    18001 cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg ccttttttct
    18061 cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca ctcggcgtga
    18121 cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg ttttcccagg
    18181 cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg cgcgcgccag
    18241 aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct tttgcctgat
    18301 cgttgttgtt gtgatgcagc gcgtagcccc aggcagctag gtagttccag cgtgcgccac
    18361 cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga tcgttccagt
    18421 catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa gtgtacggtg
    18481 cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccacgt tccgcaatcg
    18541 cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg ttgattaccg
    18601 acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta tcaccagttt
    18661 gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg cgcgttggat
    18721 catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc gccagcaaaa
    18781 atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg ttgtgatgag
    18841 caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg ctataacttg
    18901 cgggtatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg ctgacaaagt
    18961 gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa agtttgcaaa
    19021 ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg catttttgct
    19081 tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta atacagtttt
    19141 ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat agcgtcgatg
    19201 atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc gtaacctgcc
    19261 agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc gtaaggcacc
    19321 ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg accggtttct
    19381 ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat cgccacgatc
    19441 gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc ttcacggctg
    19501 aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc agccaacgtc
    19561 agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg agaagaagag
    19621 gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa gaaaccgata
    19681 gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat gcacgggaag
    19741 cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat aaccaccagc
    19801 gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt ctcgtcgcgg
    19861 aattccggga agcgcgcaga accgaggaac gtaccgccac ggttgatcat gtcagaaacg
    19921 ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc gtcataaatg
    19981 cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat tgcggcgttc
    20041 atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat gactacctct
    20101 gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat cgaagtgatc
    20161 caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag gtcaggccaa
    20221 atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga ttatgaaagc
    20281 atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat aattacatcc
    20341 gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat atgtgcctga
    20401 accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt cggccctgac
    20461 tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac gatatcaata
    20521 atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac cgcctcatat
    20581 cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa cagagcatcg
    20641 gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc gttcatcata
    20701 acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt ccggcgcacc
    20761 acccaccgct gaaacgagac aaggataatc gtacaaatta gcgccacaat tgtcacgata
    20821 accccgacgc ctggatctgt catcggtgtt ggagatacca gatgttgaat acccgtcaaa
    20881 aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc gagggactca
    20941 gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata tcgcaccacc
    21001 agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc ggcgagaata
    21061 ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcag cgaagccatc
    21121 gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt cataaatact
    21181 cccactatca actgacgcta gtataacgga agcaaatcat ctgcaatgca ttaagcagca
    21241 ggcaaattga ggataaaaaa aaccccacat catgtggggg aagacaggga tggtgtctat
    21301 ggcaaggaaa acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac
    21361 gatgaacttt tttgccattg cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc
    21421 tcgtttaaaa ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt
    21481 gccatctcaa cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca
    21541 gcgttttcat caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca
    21601 ttaacaggag gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga
    21661 tgttcggtta aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct
    21721 tcacccggat gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact
    21781 gccagcgttg aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt
    21841 tctgtcgcga ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt
    21901 gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg
    21961 tatttaaaca atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt
    22021 aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc
    22081 gcttgatctt ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa
    22141 aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt
    22201 gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta
    22261 tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg
    22321 ttcgcactgg agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga
    22381 tgccttagtg ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt
    22441 aaccggtact gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc
    22501 ccgtgaacat ttaagccagg aagtgttggg caaacgcctg acgcctttcg accgcgctat
    22561 tgatatgcac atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg
    22621 gtttaaaacc ttgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta
    22681 accgcgcgca tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg
    22741 atgttaccca agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag
    22801 gggctgatga ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta
    22861 atgtggtggc ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg
    22921 ttattggtga ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt
    22981 cgtaacttta ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc
    23041 gtggaactgg tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg
    23101 attcgtccgg ccagcagttc tcaatccgat ttcattaact tactgtttga ccgcccgcta
    23161 ttactgctga ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt ggcctggagt
    23221 ctggcaaaac cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta
    23281 cgccagcacc cggaactgga agcggggcca caggaattcc ttgccgcagg tgccagtttt
    23341 aaccagatgg tcaccgcgct ggagcgcatg atgacctctc agcagcgtct gctttctgat
    23401 atctctcacg agctgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc
    23461 cgtcgtagcg gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca acgtctggac
    23521 agcatgatca acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc gctggttagc
    23581 gaaaccatca aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc
    23641 gagcaaatgg gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc gctgtacggc
    23701 aatccgaacg ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc
    23761 catacgaaga ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac
    23821 gacgatggtc ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg
    23881 accgatgaag cacgcgatcg tgaatctggc ggtacaggat tggggctggc gattgttgaa
    23941 accgccattc agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta
    24001 cggctggtga tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga
    24061 taaggcgttt acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt
    24121 cttatcaggc ctacactccg ggggttattt tccccacaac cggcgggaaa aatcttcgat
    24181 cttgccgctc agtcggcgct tttgcatcgt ccgcgtccag cttttcgata acccggcggc
    24241 ggagagtaaa cggtgatact gatcgtcatc aaacggcata tgccatgcga tggcggcagc
    24301 ctcctgcacg gtcacatcgc tgacacggga aaccaactcc agcggcgcgt ccgcagacac
    24361 tttccctggc gctatcacgc tatacaacca ccccacctta cctgtgtttt gcatcaactg
    24421 cgctatatcg ctgatatcaa aatggtaatt gagtttatag caaggcgagc gcggctggct
    24481 gacctgaatt aatgcctctc cccagcggaa aatatcgccc atatgaacat tactttccgt
    24541 gagaccgtcg gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc
    24601 cggaaattcc cgcacccagt agagatagtg ctcacgagga taatgacaca gcgctctgtc
    24661 tggcccgccg tgaacctttt tctccgcctg ctcgtcacct tccagcccca gctccgtcag
    24721 catcaactca ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc
    24781 ctgaatcttg cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa
    24841 tttccccatt agaccatatt tattttatgt caggcgtgct gttttcgcgg taagtatcca
    24901 ggcaggaact caatagcaaa ccggacgaac gttagttcat ccggctgtgg ggattaagga
    24961 agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct ttgactttac
    25021 gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc gccgtcgcta
    25081 ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct gcaatcagca
    25141 caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca gtgccatcgc
    25201 caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata attactgcca
    25261 cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca atggtattgc
    25321 ccagcgcgaa ggcaaagaat ggaatcagcg tttgcactgc tttgctgaaa aattctcgta
    25381 attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact gcgccgacga
    25441 aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc gtcatgagcg
    25501 gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta ccgtactgct
    25561 gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc agcgccagcg
    25621 ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg cgcgaggcaa
    25681 ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg gatttacgca
    25741 gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac cacaccgcca
    25801 gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat ttccccgccc
    25861 ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc atcccccccg
    25921 ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat atttttaagg
    25981 gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat agacattcat
    26041 ttaatcaata aaagtgatca aaaccacatc aattgaaacg ctgttttatt tttatcggat
    26101 cattgttttt attttgaaat tgatcacaaa aaaaccaccg ccgttggcga tggttcattt
    26161 tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga ttattttttc
    26221 gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc tttaatgtag
    26281 tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc caggcccaga
    26341 atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc ctggttagca
    26401 gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc ggaaccaaag
    26461 cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc gaagtcacgt
    26521 tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc tttccagaac
    26581 aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt gtctgctggc
    26641 agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc tggcaggctt
    26701 tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg gtggatttcc
    26761 atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag ggatggcagg
    26821 gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc cgcgtaagca
    26881 gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc gtacttttcg
    26941 taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc acttaatgcc
    27001 gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga cagcacgatt
    27061 tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata agaagatgag
    27121 taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg cagcctgttt
    27181 ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt cagtcggtgg
    27241 gattgtttcg tggattattc tgccgtggac catcagcgcc ctgttactac cggatttctg
    27301 ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt tcggcgctat
    27361 gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca tgtcgatggg
    27421 aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc caattatcaa
    27481 cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttgc tcggcgttct
    27541 ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag agcgcaagat
    27601 gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg tgatgtgcgg
    27661 cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc atgaagccgc
    27721 tgccgcactt ggcgtcgatc cactgtatgt cgctctgcca agctatgttg tcatcatggg
    27781 cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag tgaaggattt
    27841 gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg tgttactctc
    27901 ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctggg gccacgcccg
    27961 cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct atgtattgtg
    28021 cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc gtccggtaac
    28081 ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg gcatcggcat
    28141 ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc
    28201 ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc
    28261 actgattaac aggcggctat gctggagaag atattgcgca tgacacactc tgacctgtcg
    28321 cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc
    28381 actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc
    28441 caaccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca acgaatcact
    28501 ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc
    28561 gttagcaaac gacacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc
    28621 ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg cgctaaatcc
    28681 cggaatcgct ccctgtcagt caagattcag cttcagccgc tccgggcaat aaataatatt
    28741 ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatataaaa
    28801 gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac cattaccgcg
    28861 ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat cttgcggata
    28921 acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt
    28981 taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg
    29041 gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa
    29101 tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc
    29161 tgaatccaca gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc
    29221 gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct
    29281 taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat
    29341 tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgatgcgc
    29401 tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca
    29461 agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg
    29521 caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact
    29581 gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg
    29641 gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat
    29701 catgagcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca
    29761 catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag
    29821 gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacca gacggaaaaa
    29881 aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag
    29941 taatcacgag gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg
    30001 atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat caccacaatg
    30061 aagctaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct
    30121 gtcagtaacg agaaggtcac gaattcaggc gctttttaga ctggtcgtaa tgaaattcag
    30181 caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc atccagtggg
    30241 cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat
    30301 cgttttaaca atggcctgca tagccagaac ggttatgtca cctggaatgt ggatagcctg
    30361 gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg tatcgatagc
    30421 attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg tcagcgtgtg
    30481 ggcctgcccg ttgcttatcg cgatagccgc tccaatggcc taatggcgca ggcacagcaa
    30541 caactcggca aacgcgatat ttatcaacgt agcggcatcc agtttctgcc cttcaatacg
    30601 ctttatcaac tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac
    30661 gctctgctga tgccggatta cttcagctat cgcctgaccg gcaagatgaa ctgggagtac
    30721 accaacgcca cgaccacgca actggtcaat atcaatagcg acgactggga cgagtcgcta
    30781 ctggcgtgga gcggggctaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc
    30841 attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt tgccagccat
    30901 gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgctgc ttatctctct
    30961 tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggcg
    31021 ctggcagcca acataaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat
    31081 attatgggct tatggctgct tcagcgagtg cttcaggagc ggcaaatcaa cgatctcccg
    31141 gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa tcccaatgac
    31201 gatcgcttta ttaatcctga ggcgatgtgc agcgaaattc aggctgcgtg tcgggaaatg
    31261 gcgcaaccgc tcccggaaag tgatgctgaa ctggcgcgct gcattttcga cagtctggcg
    31321 ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctac gcggtgaaga tttctcgcaa
    31381 ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg tgccgatgcc
    31441 tgcggtattc gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa tatcggcatc
    31501 cagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt cgtcagcacc
    31561 accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg
    31621 cagcttcact cgacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg
    31681 cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag gaggcgctgc
    31741 gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt
    31801 ttgaaaaccc ggaaggttcg ctgaccggtg ggattcaggc tattggtaat tatccgggca
    31861 aagcgcgtaa tgccagtgag ctacgagccg atctggaaca ggctatgcgg ctgattccgg
    31921 ggccgaaacg gcttaattta catgccatct atctggaatc agacacgcca gtctcgcgcg
    31981 accagatcaa acctgagcac ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg
    32041 gtctggattt taacccctcc tgtttctcgc atccgctaag cgccgatggc tttacgctct
    32101 cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc agccgtcgcg
    32161 tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc tggatcccgg
    32221 atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg ctggcagcac
    32281 tggatgaggt gatcagcgag aagctggatc cggcgcacca tatcgacgcc gttgagagca
    32341 aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt tacatggggt
    32401 atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcatttccac ccgactgaag
    32461 tgatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg ctgcacgtca
    32521 gccgtccggt tcgctgggac agcgatcacg tggtgctgct ggatgatgaa acccaggcga
    32581 ttgccagtga gattgttcgt cacgatctgt tcgaccgggt gcatatcggc ctcgacttct
    32641 ttgatgcctc tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag
    32701 ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg caagctggaa gcggcgggcg
    32761 attacactgc gcgtctggcg ctactggaag aacagaaatc gttgccgtgg caggcggtct
    32821 gggaaatgta ttgccaacgt cacgatacgc cagcaggtag cgaatggctg gagagcgtgc
    32881 gggcttatga gaaagcgatt ttgagccagc gtgggtaaac actgccggat gcggcgcgag
    32941 cgccttatcc ggcctacagg tcgaatgacc atgcgtgctg ccaccctcac cccggccctc
    33001 tccctgaaag ggcgagggag aaaaacatag cctcgtgcgg caacggagct gaacaccgct
    33061 ccggacagtc ccctcgcccc tacggggaga gggttagggt gaggggaaca agccgcacca
    33121 tgtctaaaca aggcaaccta atttacagga acacagaaca tgcaaaacat tacccagtcc
    33181 tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg ctgggacgag
    33241 cgcaatggtg gtaacctgac gctgcgcctg gatgacgccg atatcgcgcc ttatcacggc
    33301 aactttcatg cacaaccgcg ctatattccc ctcagccaac ccatgccttt actggcaaat
    33361 acgccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct tgatcctgca
    33421 gctaacttag gcgtcgtaaa agtcgacagc gacggcgcgg gctaccatat tctctggggg
    33481 ttaaccaacg aagccgttcc aacttccgaa cttccggctc acttcctttc ccactgcgag
    33541 cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc caccaacctg
    33601 atcgccctca cctatgtact tgaaaacgac accgcggtct ttactcgcca actgtgggaa
    33661 ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc gtggatggtg
    33721 cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca ttcgctggtg
    33781 ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac attcggttta
    33841 atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat gggcggcatg
    33901 aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg cgttacacca
    33961 ctagctagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt tgtcatgcag
    34021 gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg gccggaactg
    34081 gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga caaagcgcgt
    34141 aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc ggtagccagc
    34201 actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc taacccggat
    34261 aacagcccgg tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt gcccaatgtg
    34321 ccagagatgc ctccggcaca atattttaaa tttcgatttg aaaaattaaa catattacat
    34381 ctcccggtaa gttatatttc cctgatacat tgtgagtaaa tcacaaaaat aatgaataac
    34441 ccattaatga ttcatgtggt ttatttaaat aaccattatg tgcattactc cgcaaatctg
    34501 acctttcact ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc atcacttaat
    34561 cgctccacca ttacaattga ttaaaatata ttttatggag tccgattaat ggcagctctt
    34621 actgcaagct gtattgacct gaatattcag ggcaatggcg cttattccgt tctgaagcag
    34681 ttggcgacaa tagcgttaca aaacggtttt atcaccgact cacaccagtt cctgcaaacc
    34741 ctgctgctgc gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc cgtgccgcac
    34801 ggtaaaagcg cctgcgttaa acaaccgttt gtattattcg cccgcaaagc gcaggctatt
    34861 gactggaaag ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg cgtgccgcaa
    34921 agcggcgaag aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat tatccaccag
    34981 gattttattc atcaactgca acagggcgac gccgaccagg tgcttgcctt gttaaatcaa
    35041 accctcagct cataaggaag tggcgatgga gtcatcctta tgtattgtcg cgatcaccaa
    35101 ctgccccgcc gggatcgctc acacctacat ggtggcggaa gccctggaac agaaagcccg
    35161 ttctctcggc cataccataa aagtggaaac tcaagggtcc agtggcgttg aaaaccgctt
    35221 atccagcgaa gagattgccg ctgccgatta cgtcattctc gctaccgggc gtggcctgag
    35281 cggtgatgat cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca tctcccaggc
    35341 gttgaaaaat atcgaccaga ttttcagcga attaccgaca aactcgcagc tttttgccgc
    35401 agatagcggc gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga tgagccacct
    35461 gatggcgggc gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc tggtggcgct
    35521 tgccaacatg ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag gcgcgccgtc
    35581 gtttacctgg gtagttgaat ccatcggtta ccttggcttc acctttatga tccccatcat
    35641 gggcgcttat attgcctcgt cgattgccga taaaccggct ttcgctccgg cgtttctggt
    35701 ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg gattcctcgg
    35761 cgcggtggtg ctggggctgg cgattggcta tttcgtcttc tggttccgca aagtgcgcct
    35821 tggcaaagca ttacaaccgc tgctcggttc aatgctgatc ccgttcgtta ctctgctggt
    35881 tttcggcgtg ctcacttact acgttatcgg gccggtgatg tccgacctca tgggcgggct
    35941 gctccacttc ctgaatacca ttcctccatc aatgaagttt gcggcagcgt ttctggtcgg
    36001 tgcgatgctg gcgttcgata tgggcggccc catcaataaa accgcctggt tcttctgctt
    36061 ctcactgctg gaaaaacaca tttatgactg gtacgccatc gtcggcgttg tcgcgttgat
    36121 gccgcccgtt gcggcgggtc ttgcgacgtt cattgcgccc aaactgttta cccgacagga
    36181 aaaagaggct gccagtagtg ccattgtggt cggtgccaca gtcgcaaccg aaccggctat
    36241 accttacgct ctggcagctc cgttgccgat gattaccgcc aatacgctgg cgggtggcat
    36301 taccggcgtg ctggtgatcg ctttcggaat caaacgtctg gcacctggtt tgggtatctt
    36361 tgacccgctg atcggcctga tgtcgccggt gggttcgttt tatctggtgc tagcgattgg
    36421 cctcgcgctg aacatttcat tcattatcgt cctgaaagga ttgtggttac gccgcaaagc
    36481 gaaagccgcg caacaggagc ttgtccatga acattgagtt actgcaacag ttgtgcgaag
    36541 ccagcgccgt cagcggcgat gaacaggaag ttcgcgacat tctgataaac acgctggaac
    36601 cttgcgttaa tgagatcacc tttgatggtc tgggcagctt tgttgcccgt aaggggaata
    36661 aaggtccaaa agttgccgtt gtcaggcata tggatgaagt cggctttatg gtcacccaca
    36721 tcgacgagag cggttttctg cgctttacca ccattggcgg ctggtggaat cagtcaatgc
    36781 tcaaccaccg ggtaaccata cgcacacaca agggatttaa aatccctggt gtgattggtt
    36841 ccgtcgcacc tcatgcgtta acggaaaagc aaaagcaaca gccgctgtca tttgatgaga
    36901 tgttcattga tattggcgcg aacagtcgcg atgaggtgga aaaacgcggt gtggcgatgg
    36961 gcgattttat tagcccggaa gccaattttg cctgctgggg cgaagataaa gtggtcggca
    37021 aggcgctgga taaccgcatc ggctgcgcaa tgatggctga actattgcag acggtgaata
    37081 atcccgaaat tacgctgtat ggcgtcggca gcgtggaaga agaagttggg ctacgcgggg
    37141 cacaaacctc ggcggaacac attaaaccgg atgtggtgat tgtgctggat accgctgtcg
    37201 caggtgatgt tccgggcatt gataacatta aatacccgct gaaactgggc caggggccgg
    37261 ggctgatgct gtttgacaag cgctacttcc ccaaccagaa actggtggcg gcgttaaaaa
    37321 gctgtgccgc acataacgat ttaccgctgc aattttccac catgaaaacc ggtgcgacgg
    37381 atggcgggcg ctacaacgtg atgggcggcg ggcgtccggt tgttgcgctg tgcctgccaa
    37441 cccgctatct gcacgctaac agcggcatga tttcaaaagc cgattacgat gctctgctca
    37501 cgttgatacg ggattttctg accaccttaa ccgcggagaa agtcaacgcg tttagccagt
    37561 tccgtcaggt ggattaatgc taaacgaacg ccagttaaag attgtcgatc tgctggagca
    37621 acagccgcgc acgcctggtg agctggcgca acagactggc gtttcaggca ggaccatcct
    37681 gcgtgatatt gactatctca actttaccct taacggcaaa gcccgcattt tcgccagtgg
    37741 cagtgcgggc tatcagctgg aaatcttcga gcgccgcagc ttttttcagt tgctgcaaaa
    37801 gcacgataac gacgatcggc tgctggcgct gttattactg aataccttta ctccccgtgc
    37861 gcaactcgcc tcggcgctta atttgccaga aacctgggta gcagagcgtc tgccccggtt
    37921 aaaacagcgt tatgaacgca cttgttgcct ggccagtcgc cccggtttgg gccatttcat
    37981 tgatgagaca gaagagaaac gcgttatctt gctggcgaac ttgctgcgca aagatccgtt
    38041 tttaattccg ctggcgggcg taacacgaga caattttcag catttatcca cggcctgcga
    38101 caaccaacac cgctggccgc tcatgcaggg tgattatctc tccagcctga ttctggcgat
    38161 ttacgccctg cgtaatcaac tgaccgatga gtggccgcaa tatcccggta acgagataaa
    38221 acaaatcgtt gaacagagcg gcatgtttct tggtgataac gctgtaagaa ccctgacagg
    38281 tttgatagag aaacagcatc agcaagcgca ggtaatttca gccgatcatg tgctggggtt
    38341 gctgcaaagg gtgccaggca tcgcgtcatt gaatattatt gatacgcagc tggttgagaa
    38401 tattaccggg catttattac gttgccttgc cgcaccggtg tggattgccg agcaccgcca
    38461 aagcagcatg aataacctga aagccgcctg gcctgcggcc tttgatatga gtctgcactt
    38521 tattacgcta ctgcgtgaac agctcgatat tccccttttc gatagcgatc tgatcggttt
    38581 gtattttgcc tgtgcgctgg aacggcatca aaacgaacgc cagccgatca ttttgctctc
    38641 ggaccagaac gcgattgcca ctattaatca gctcgccatt gagcgcgatg ttttacattg
    38701 tcgggtgatt attgcccgta gcttaagcga gcttattgcc attcgcgaag agattgagcc
    38761 gttattgatc attaacaaca gccattattt actggatgac gcggtaaata attacatcac
    38821 cgtaaaaaat atcattacgg ctgccgatat cgaacaaata aaacactttc tggcgacggc
    38881 atttattcgc caacagccgg aacgcttttt ctctgccccc ggaagttttc attattcgaa
    38941 tgtacgcggt gaaagctggc aacatattac ccggcaaatt tgtgcgcaat tagtggcaca
    39001 acaccatatt accgccgatg aagcgcaacg gatcatcgcc cgcgaaggcg aaggtgaaaa
    39061 cctgattgtt aatcgcctcg ccatcccaca ttgctggagc gaacaggagc gacgttttcg
    39121 tggatttttt attaccctcg cccaaccagt tgaggtgaat aacgaagtca ttaaccatgt
    39181 cttgatcgcc tgcgccgccg ccgatgcgcg tcatgagcta aaaatattta gctatctggc
    39241 aagcgtattg tgtcagcatc cggcagaagt tattgccggg ttaacaggat atgaggcatt
    39301 tatggagtta cttcacaagg ggtgaactgt gtcggatgcg ccgcatccga cactgcattt
    39361 tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg tactcgcgta
    39421 acgctcgtgg aaatgagtct tcgaccatcc agcttgtgtt tgcatcctga agatgctctt
    39481 tatcccagtc agagtacggg actttatctc tgacgcggcg aacatatttt ttagtctcga
    39541 gttgatactg gtctgccgaa ttaatgaacg agaaattcat accgccttta tccgcttctt
    39601 tggcctgcgc taccagcgtt tccagttcag agactttctt cattgccgca tcaacatcaa
//
LOCUS       DAJVXP010000021        39409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVXP010000021 DAJVXP010000000
VERSION     DAJVXP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.021"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..266
                     /locus_tag="OQS10_001092"
     CDS             <1..266
                     /locus_tag="OQS10_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS2292177.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OQS10_001093"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OQS10_001093"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS2292178.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQS10_001094"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OQS10_001094"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS2292179.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OQS10_001095"
     CDS             complement(1570..3270)
                     /locus_tag="OQS10_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS2292180.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OQS10_001096"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OQS10_001096"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS2292181.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OQS10_001097"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OQS10_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS2292182.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OQS10_001098"
     CDS             4828..6600
                     /locus_tag="OQS10_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS2292183.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OQS10_001099"
     CDS             6593..8374
                     /locus_tag="OQS10_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS2292184.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OQS10_001100"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OQS10_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS2292185.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OQS10_001101"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OQS10_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS2292186.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQS10_001102"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OQS10_001102"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS2292187.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OQS10_001103"
     CDS             11336..11908
                     /locus_tag="OQS10_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS2292188.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OQS10_001104"
     CDS             complement(11939..12250)
                     /locus_tag="OQS10_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS2292189.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OQS10_001105"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQS10_001105"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OQS10_001106"
     CDS             12629..12982
                     /locus_tag="OQS10_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS2292190.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQS10_001107"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OQS10_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OQS10_001108"
     CDS             complement(14736..15206)
                     /locus_tag="OQS10_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS2292191.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQS10_001109"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OQS10_001109"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS2292192.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQS10_001110"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OQS10_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS2292193.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQS10_001111"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OQS10_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS2292194.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OQS10_001112"
     CDS             complement(17208..20357)
                     /locus_tag="OQS10_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS2292195.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQS10_001113"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OQS10_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS2292196.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OQS10_001114"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OQS10_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS2292197.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OQS10_001115"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OQS10_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS2292198.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGISWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQS10_001116"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OQS10_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS2292199.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQS10_001117"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OQS10_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS2292200.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OQS10_001118"
     CDS             26836..27213
                     /locus_tag="OQS10_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS2292201.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OQS10_001119"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OQS10_001119"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS2292202.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OQS10_001120"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OQS10_001120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS2292203.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OQS10_001120"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OQS10_001121"
     CDS             30024..30353
                     /locus_tag="OQS10_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS2292204.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OQS10_001122"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OQS10_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS2292205.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OQS10_001123"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OQS10_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS2292206.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OQS10_001124"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OQS10_001124"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS2292207.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OQS10_001125"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OQS10_001125"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS2292208.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQS10_001126"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OQS10_001126"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS2292209.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OQS10_001127"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OQS10_001127"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS2292210.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OQS10_001128"
     CDS             complement(37406..39082)
                     /locus_tag="OQS10_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS2292211.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9527 a        10050 c        10417 g         9415 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcatc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAJVXP010000022        39389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVXP010000022 DAJVXP010000000
VERSION     DAJVXP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.022"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(147..1487)
                     /gene="fadL"
                     /locus_tag="OQS10_001129"
     CDS             complement(147..1487)
                     /gene="fadL"
                     /locus_tag="OQS10_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS2292212.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1859..2143
                     /locus_tag="OQS10_001130"
     CDS             1859..2143
                     /locus_tag="OQS10_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS2292213.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2323..3633
                     /gene="fadI"
                     /locus_tag="OQS10_001131"
     CDS             2323..3633
                     /gene="fadI"
                     /locus_tag="OQS10_001131"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS2292214.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3633..5777
                     /gene="fadJ"
                     /locus_tag="OQS10_001132"
     CDS             3633..5777
                     /gene="fadJ"
                     /locus_tag="OQS10_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS2292215.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            5980..6465
                     /gene="sixA"
                     /locus_tag="OQS10_001133"
     CDS             5980..6465
                     /gene="sixA"
                     /locus_tag="OQS10_001133"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS2292216.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7149..7712
                     /locus_tag="OQS10_001134"
     CDS             7149..7712
                     /locus_tag="OQS10_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2292217.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7794..10436
                     /gene="yfcU"
                     /locus_tag="OQS10_001135"
     CDS             7794..10436
                     /gene="yfcU"
                     /locus_tag="OQS10_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS2292218.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10456..11207
                     /locus_tag="OQS10_001136"
                     /pseudo
     CDS             10456..11207
                     /locus_tag="OQS10_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11182..11730
                     /locus_tag="OQS10_001137"
     CDS             11182..11730
                     /locus_tag="OQS10_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292219.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11727..12197
                     /locus_tag="OQS10_001138"
     CDS             11727..12197
                     /locus_tag="OQS10_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292220.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12194..12718
                     /locus_tag="OQS10_001139"
     CDS             12194..12718
                     /locus_tag="OQS10_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292221.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12705..13577
                     /locus_tag="OQS10_001140"
     CDS             12705..13577
                     /locus_tag="OQS10_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS2292222.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13699..14250)
                     /gene="smrB"
                     /locus_tag="OQS10_001141"
     CDS             complement(13699..14250)
                     /gene="smrB"
                     /locus_tag="OQS10_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS2292223.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14416..15348
                     /gene="prmB"
                     /locus_tag="OQS10_001142"
     CDS             14416..15348
                     /gene="prmB"
                     /locus_tag="OQS10_001142"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS2292224.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15383..16468
                     /gene="aroC"
                     /locus_tag="OQS10_001143"
     CDS             15383..16468
                     /gene="aroC"
                     /locus_tag="OQS10_001143"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS2292225.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16472..17296
                     /gene="mepA"
                     /locus_tag="OQS10_001144"
     CDS             16472..17296
                     /gene="mepA"
                     /locus_tag="OQS10_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS2292226.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17296..18105
                     /locus_tag="OQS10_001145"
     CDS             17296..18105
                     /locus_tag="OQS10_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS2292227.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18105..18653
                     /gene="epmC"
                     /locus_tag="OQS10_001146"
     CDS             18105..18653
                     /gene="epmC"
                     /locus_tag="OQS10_001146"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS2292228.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18687..18965
                     /locus_tag="OQS10_001147"
     CDS             18687..18965
                     /locus_tag="OQS10_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS2292229.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19086..21092)
                     /gene="mnmC"
                     /locus_tag="OQS10_001148"
     CDS             complement(19086..21092)
                     /gene="mnmC"
                     /locus_tag="OQS10_001148"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS2292230.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21251..22471
                     /gene="fabB"
                     /locus_tag="OQS10_001149"
     CDS             21251..22471
                     /gene="fabB"
                     /locus_tag="OQS10_001149"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS2292231.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22746..23924
                     /locus_tag="OQS10_001150"
     CDS             22746..23924
                     /locus_tag="OQS10_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292232.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(23921..24916)
                     /gene="flk"
                     /locus_tag="OQS10_001151"
     CDS             complement(23921..24916)
                     /gene="flk"
                     /locus_tag="OQS10_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS2292233.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25015..26151
                     /gene="pdxB"
                     /locus_tag="OQS10_001152"
     CDS             25015..26151
                     /gene="pdxB"
                     /locus_tag="OQS10_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS2292234.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26217..27230
                     /locus_tag="OQS10_001153"
     CDS             26217..27230
                     /locus_tag="OQS10_001153"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS2292235.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27230..28042
                     /gene="truA"
                     /locus_tag="OQS10_001154"
     CDS             27230..28042
                     /gene="truA"
                     /locus_tag="OQS10_001154"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS2292236.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28125..28784
                     /locus_tag="OQS10_001155"
     CDS             28125..28784
                     /locus_tag="OQS10_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2292237.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            28940..29854
                     /gene="accD"
                     /locus_tag="OQS10_001156"
     CDS             28940..29854
                     /gene="accD"
                     /locus_tag="OQS10_001156"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS2292238.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            29924..31192
                     /gene="folC"
                     /locus_tag="OQS10_001157"
     CDS             29924..31192
                     /gene="folC"
                     /locus_tag="OQS10_001157"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS2292239.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31182..31844
                     /gene="dedD"
                     /locus_tag="OQS10_001158"
     CDS             31182..31844
                     /gene="dedD"
                     /locus_tag="OQS10_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS2292240.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32103..32591
                     /gene="cvpA"
                     /locus_tag="OQS10_001159"
     CDS             32103..32591
                     /gene="cvpA"
                     /locus_tag="OQS10_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS2292241.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32628..34145
                     /gene="purF"
                     /locus_tag="OQS10_001160"
     CDS             32628..34145
                     /gene="purF"
                     /locus_tag="OQS10_001160"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS2292242.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34240..34809
                     /gene="ubiX"
                     /locus_tag="OQS10_001161"
     CDS             34240..34809
                     /gene="ubiX"
                     /locus_tag="OQS10_001161"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS2292243.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35075..35857
                     /gene="argT"
                     /locus_tag="OQS10_001162"
     CDS             35075..35857
                     /gene="argT"
                     /locus_tag="OQS10_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS2292244.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36077..36859
                     /gene="hisJ"
                     /locus_tag="OQS10_001163"
     CDS             36077..36859
                     /gene="hisJ"
                     /locus_tag="OQS10_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS2292245.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            36949..37635
                     /gene="hisQ"
                     /locus_tag="OQS10_001164"
     CDS             36949..37635
                     /gene="hisQ"
                     /locus_tag="OQS10_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS2292246.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37632..38348
                     /locus_tag="OQS10_001165"
     CDS             37632..38348
                     /locus_tag="OQS10_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2292247.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38356..39129
                     /gene="hisP"
                     /locus_tag="OQS10_001166"
     CDS             38356..39129
                     /gene="hisP"
                     /locus_tag="OQS10_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS2292248.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9323 a         9654 c        10591 g         9821 t
ORIGIN      
        1 gataccgctt attgatttga aagggcaagc tgccaacagg ctgtttgcaa aactcaggag
       61 gtaagcaatg aggacactac tttcggtgaa gtgggggagt taaagtcacc tgctatgcag
      121 gtgactttat ccaggcgaac gcgttatcag aacgcgtagt taaagttagt accgaacagc
      181 caggctttac cttcagactc gaactggtat gggccttcgt taattttcac gctctgaccg
      241 tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta agtcgtacct
      301 gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg agccggaact
      361 gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta gtaataagtg
      421 gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa cagcgtgtcg
      481 ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta agccaggcta
      541 tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca catttcaggc
      601 aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg aattggcaaa
      661 ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa gtcaattttc
      721 acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata caggattccg
      781 gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt attactgtcg
      841 ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc aggagattgc
      901 ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat tttcgcgcga
      961 gcgtagacgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg atacgcaccg
     1021 cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc gcctgcataa
     1081 gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc ccaaccaaat
     1141 tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg cgcgatgtta
     1201 tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc cgggtcaata
     1261 taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc ggggttacgg
     1321 ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg gcccaggcca
     1381 gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga gataagtgcc
     1441 actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac cataacctca
     1501 atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa gtgtagggtc
     1561 tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca gctggaatgc
     1621 tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc agctggcaaa
     1681 atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat ttttaagtgt
     1741 gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact ttccggaagc
     1801 gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga aatcaacgat
     1861 gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg gcaccattat
     1921 ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaaaccgcg cagaagcaga
     1981 acaaacgctg gcggcgctga ctgaaaaagc ccgtagcgtg gaatccgaac cgtgcaaaat
     2041 ctccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt tcacttttgc
     2101 ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct cgggtacgtt
     2161 cgcccctgtc agttgctggc aggggcgttt tcttttcttt ctcctgtctt cactgtgtca
     2221 tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatagtt aagactatga
     2281 tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca ggttttaccg
     2341 ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac gccttttgcc
     2401 cgccaggcga cggcttttca tggcattccc gcggttgatt tagggaagat ggtggtaggc
     2461 gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt ctttggtcag
     2521 gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg taccggaatg
     2581 aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt ccaggcggtt
     2641 gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc cggtggggca
     2701 gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgcgcgt gctggttgat
     2761 gtcaacaaag ctcgaaccat gagccagcgc ctgaaactct tctctcgcct gcgtttgtgc
     2821 gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg gatgggcgac
     2881 accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga tgcattagcg
     2941 caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa agaagaggtg
     3001 atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa tattcgcggt
     3061 aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa acacggaacg
     3121 gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcagtgat cctgatgact
     3181 gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag ctacgcattt
     3241 actgcgattg atgtctggca ggacatgttg ctcggtccag cctggtcaac accgctggcg
     3301 ctggaacgtg ccggtttgac gatgagcgaa ctgacattga tcgatatgca cgaagccttt
     3361 gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc tcgtgaagta
     3421 ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt gcttggcggt
     3481 tcgattgctt acgggcatcc cttcgcggcg accggcgcgc ggatgattac ccagacattg
     3541 catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc tgccggtggg
     3601 cttggcgcgg caatggttct ggaggcggaa taatggaaat ggcatcagcg tttactctta
     3661 atgttcgtct ggacaacatt gctatcatca ccatcgatgt accggatgag aaaatgaata
     3721 ccctgaaggc ggaatttgcc tcgcaggtgc gcgccattat taagcaactc cgtgaaaaca
     3781 aagagttgcg cggcgtggtg tttatctccg cgaaaccgga caattttatc gccggagcag
     3841 acatcaacat gattggcaac tgcaaaacgg cgcaagaagc ggaagcactg gcgcggcagg
     3901 gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg gctattcatg
     3961 gcgcttgcct gggcggtggg ctggagctgg cgctggcgtg ccacggtcgc gtttgtactg
     4021 acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta cccggttcag
     4081 gcggcaccca gcgtttaccg cgtctgatag gcgtcagtac agcattagag atgattctca
     4141 ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat gacgttgttc
     4201 cgcactccat tttgctggaa gccgctgttg agctggcgaa gaaggatcgc ccatcttccc
     4261 gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg ctgctgttca
     4321 aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg acagaacgca
     4381 tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggctat gacgctgaag
     4441 ctcgcgcctt tggcgaactg gcgatgacgc cgcaatcgca agctttgcgt agtatctttt
     4501 ttgccagtac ggacgtgaag aaagatcccg gtagtgatgc accgcctgcg ccattaaaca
     4561 gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc actgcttgta
     4621 aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat catgcgctga
     4681 agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa gccagcgaac
     4741 gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt gcccatcgcg
     4801 atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg gtggcggaag
     4861 tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa cacgtcatct ttaccgattg
     4921 gtgatatcgc cgctcacgcc gcgcgacctg agcaagttat tggcctgcat ttcttcagtc
     4981 ctgtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg gcgcaaacca
     5041 tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc gtgcgtgaca
     5101 aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct atccgcatgc
     5161 tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt ggttttccgg
     5221 taggcccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa attattcctg
     5281 tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt tcttcaattt
     5341 tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat ggtcagaaag
     5401 ggcgtaaaag caaaaaacag gtcgatcccg ccatttaccc gctcattggc gcacaagggc
     5461 aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg ctgaatgaag
     5521 cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat attggcgcgg
     5581 tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc gattctctcg
     5641 gcgcgggcga agtggttgca ataatgcaac gactggccac gcagtatggt tcccgtttta
     5701 ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg aaaacaactg
     5761 caactgacct tcaataagaa ggtcaaagct atatgaatcc gcgctgaatg gcggagtgtt
     5821 ggtcaaaatg taaacgcata ttgactatac ttacgccatt gaggtaaaaa acagcgtttc
     5881 attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg tttcagatga
     5941 aagaaaacgg acgaatctgg ttaacaaaag cggtgcaata tgcaagtttt tatcatgcgt
     6001 cacggcgacg cagcccttga tgccgccagt gattcggttc gtcctctgac cactaatggt
     6061 tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga aatcgaacgc
     6121 gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg ggattgtctg
     6181 aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga tgttggtctg
     6241 gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact ggtgatctct
     6301 cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac gccgcctatg
     6361 tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg cacattcaac
     6421 tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgagt tccaaattat
     6481 gcccaatgag gtagattcat tgggcataat tgctcatcag gcagcggata tctgtcaatc
     6541 tcactactat cgaagactct ccccatctat aaataaaaca ctttctactt tcattcggaa
     6601 attattcttc tgcgcgtatc ttgattttgc ccaaagttac atgttgaaat gaataatgtc
     6661 tgtttgacac tcattgtcat ttattaatac agataataga gaaaatagag ttgaataatc
     6721 aggcaggatg taattgaaac ttgattaaat tctggctata gtgtttatat attatattgt
     6781 caaaagtgga taaatgccaa tcgcattata tgttcattgt ttgtttttta agctgttgat
     6841 attcattatt gatatacttt ctattttttc taaatactta ttccgtattt tttaaggatg
     6901 tccttaagat aacctttctt ctgtcaatat attaattaaa tctacattta ctctttagta
     6961 aaattaaatt gcatcagggg tgtggaaaca tcactatgcg gccccacacc aacgaataca
     7021 attttctacg acctgttctt tttggatcgc atgccgtatt caatggctgg gtataaacat
     7081 tggttattga ctgaatcaat gtattgcaat gtttatattt ttaattattt atagtttaag
     7141 gacaaataat gagtaagttt gttaaaacag ctattgctgc ggcaatggtg atgggcgtgt
     7201 tcacctctac ggcaacaatc gctgcgggta acaacggtac tgcacgtttt tacggcacca
     7261 ttgaagactc agtgtgttct atcgttccgg acgatcacaa actggaagtt gatatgggtg
     7321 atatcggtgc cgaaaaactg aaaaataacg gcaccaccac gccgaaaagt ttccagattc
     7381 gtctgcaaga ttgcgtattt gatactcagg aaacaatgac cactaccttt actggtaccg
     7441 tttcttctgc aaatagcggc aattattaca ccattttcaa taccgatacc tgtgcggcat
     7501 ttaacaatgt tagcctggcg atcggtgact ctctgggtac ctcttacaaa agcggcatgg
     7561 gtattgacca gaaaatagtg aaagacactt ctaccaataa aggcaaagcg aagcagacac
     7621 tgaactttaa agcctggctg gtcggcgcag ctgatgcgcc agatctgggt aatttcgaag
     7681 caaataccac cttccagatt acttacctgt aaggcatacc cccatagcga cactgctatg
     7741 ggggatttaa atggattttg accgaacaag aataaaaacg gatagaaacg tgtatgcctg
     7801 accattctct ttttcgatta cggattcttc cgtggtgcat tgcgctggca atgtcaggga
     7861 gttatagcag tgtctgggct gaagacgaca ttcagtttga ttcccgtttt ctggaattaa
     7921 aaggcgacac aaaaattgat ctgaagcgtt tttccagcca gggatatgtt gagcccggaa
     7981 aatacaattt acaggttcaa ctaaataaac agccattggc ggaagagtac gatatttact
     8041 ggtatgctgg tgaagatgac gcgagcaaaa gctatgcttg tctgacaccg gaactggtag
     8101 cgcagtttgg tttaaaagaa gacgtggcga aaaatctgca atggagccac gatgctaaat
     8161 gcctgaaatc cggtcaactg gaaggcatgg aaattaaggc tgatttaagc cagtccgcat
     8221 tagtcatttc actgccacag gcttacctcg aatatactta tcccgactgg gatccgcctt
     8281 cacgttggga tgacggcatc tccgggatcg tcgcggacta cagcatcaac gcacaaaccc
     8341 ggcacgaaga aaatggcggt gatgatagta acgagatcag cggcaacggg acggtcgggg
     8401 ttaacctggg gccgtggcgt atgcgtgctg actggcagac taactatcaa catactcgca
     8461 gtaatgatga cgatgaattc agcggcgatg aaactcaaaa aaaatgggag tggagtcgct
     8521 actatgcctg gcgggcgtta ccatcattaa aagccaaact ggcgctgggc gaggattacc
     8581 tcagatccga tatttttgat ggttttaact atgttggtgg cagtgtcagt actgacgatc
     8641 aaatgttgcc tcccaatctg cgcggctacg cgccagacat ttccggcgtg gcacacacca
     8701 cagcaaaagt gaccgtcagc cagatggggc gtgtgattta cgaaacgcag gttccggctg
     8761 gaccgtttcg tattcaggat cttggtgatt ctgtctccgg tacgttgcat attcgcattg
     8821 aagaacagaa cggccaggtg caggaatatg acatcagcac cgcctcgatg ccatacctca
     8881 ctcgtccagg tcaggttcgc tataagatca tgatgggccg tccgcaagag tggggacacc
     8941 atgtcgaggg tgaatttttt tctggtgctg aagcttcctg ggggatcgct aacggctggt
     9001 cgttatatgg cggcgcactg ggagatgaaa actatcagtc tgcggcgctt ggcgtcggtc
     9061 gcgatttgtc tacattcggc gcggtcgcgt ttgatgttac tcactcgcac accaaactgg
     9121 ataaagacac cgcttatggc aaaggttcgc tggacggtaa ctccttccgt gtgagttatt
     9181 ccaaagactt tgaccagctc aacagccgcg ttacttttgc tggatatcgc ttctcggaag
     9241 agaactttat gaccatgagc gagtatctgg atgccagtga cagcggaatg gtacgcacgg
     9301 gcaacgacaa agagatgtac actgccactt ataaccagaa cttccgcgat gcgggtgttt
     9361 cggtttatct caactatacc cgccatacct actgggatcg cgaggagcag acaaactaca
     9421 acatcatgct ctcgcactat ttcaatatag gcagtattcg taatgtcagc atctcgatga
     9481 ctggctaccg ttacgagtat gacaaccagg ccgacaaagg catgtacatt tcgctcagta
     9541 tgccgtgggg tgacaacagt accgttagct ataacggtaa ctatggcagt gggacggaca
     9601 gcagtcaggt cggttatttc agccgtgtcg atgacgcgac tcactatcag ttgaacgtcg
     9661 gcaccagtga caaacacacc agcgttgacg gctattacag ccatgatggt tcgctggcgc
     9721 aggttgacct cagtgcgaac taccatgaag ggcaatacac ctctgcgggc ttgtcgttac
     9781 agggcggcgc aacgcttact gcccacggtg gcgcacttca ccgtacccag aatatgggcg
     9841 ggacacgctt gttgattgat gccgatggcg ttgccgatgt tccggtagaa ggtaacggtg
     9901 ctgctgttta taccaatatg tttggtaaag ccgtcgtttc tgacgtcaat aactattacc
     9961 gcaatcaggc gtatatcgac ctcaacaaac tgccggaaaa cgccgaagca acccagtcgg
    10021 tggtacaagc cacgctaact gaaggggcga ttggctaccg caaatttgcc gtcatcagtg
    10081 gtcaaaaagc gatggcggtg ctgcgtttac aagacggcag ccatcctccg tttggcgcag
    10141 aagtaaaaaa tgataacgag cagacagtgg gccttgtcga tgatgacggc aatgtttatc
    10201 tggctggggt gaaacctggc gaacacatga gtgtgttctg gagtggtgtt gcgcattgcg
    10261 atatcaacct gccggacccg ctgcctgccg atctgtttaa cggcttgtta ctgccatgcc
    10321 agcataaagg caacgtcgcg ccggttgttc ctgatgacat aaagcctgtc attcaggagc
    10381 agacgcaaca ggtgacaccc accaatcccc ctgtttctgt ttcagctaac caataacgtg
    10441 attaaggaat gatccatgtc tgatttactt tgctctgcca aattgggtgc tacgacgcta
    10501 gcgttactgc ttagcgctgc cagtttgtcg cccaggcttc tgtaacgcca gatcgtaccc
    10561 gtttgatatt taatgaaagc gacaaatcaa tcagcgtgac gttgcgcaat aacgatccga
    10621 aactgcctta tctggcccaa agctggatcg aagatgaaaa gggtaacaag atcagttcac
    10681 cgttaactgt tctgccaccg gtgcaacgca tcgattcaat gatgaacggg caggtaaaag
    10741 tgcagggaat gccggatatc aacaaattgc ctgccgatcg tgaaagcctg ttctacttca
    10801 acgtgcgtga gatcccgcca aaatcaaaca aagccaacac gctgcaaata gcactacaaa
    10861 cgcgtattaa gttgttctgg cgaccgaagg cgctggagaa cgtcagcatg aaaaacccct
    10921 ggcaatataa ggttacttta acccgcaacg gtcaggaatt taccgtcaat aacccgacgc
    10981 cgtattacgt cattttcagt aatgccagca cacagaaaaa tggcaatcct gcagccggtt
    11041 tctcgccaat agtgatgtcg ccgaaaacct ctgcaccgct gaatgtgaag atgggtagcg
    11101 tgccggtact gacctatgtc aacgactatg gcgctcggat gccgctcttt ttcacttgca
    11161 acggtaacag ttgccaggtc gatgaagagc agagccgcaa aggctgaggc cgggagagcc
    11221 agtatgaaag gatcttgttc tgtctgggcg gtaactgggt tactgatatg ttgtgttggt
    11281 agcggctttg cagttgcagc gagtaagtca gtaagtatga atttacgggt gcttgtcgat
    11341 gcccctcctc cttgtacggt aaatggtgcg gcagttgagt ttggtaatgt attcattaat
    11401 aagatcaatg gggttgatta taaacgtccc attgactact cgcttgtttg taacaatctg
    11461 gcaatggatg acctgcgtct acagatgcag gcgacaacgg tcgtgattaa cggtgaaaca
    11521 gttatcagca cgggtattcc tgggtttggt attcgggttc agaaaagtag tgatcacaca
    11581 atactcgact taacctctgg ctcatggttg ccatttaatt tttcctcggg agttcccgtt
    11641 ctggaagctg tgccggtgaa acagagtggt acaacgttag cggcagctga atttaacgcc
    11701 agtgccacaa ttgtggtgga ttaccaatga aacggatctc gcttattttg ctgtggggct
    11761 tttgctcgat ggcactctca aacgtctctt ttcacggtta tttagtgcag ccgcctaact
    11821 gcacgattag caacgctcaa accattgaaa taacttttca ggatgtgctt attgatgaca
    11881 ttaatggcag caattatgag caaaccgtgc cgtattccat tacctgcgat acggcggttc
    11941 gcgacccatt aatggaaatg accttgagct ggagtggcac actaagcgat tttgataatg
    12001 ctgcagtaag cagcaatatc actggtttag gcattcagct caaacaggca gggcagagtt
    12061 ttacgatcaa tacgccactt atggtgaatg aaaccgattt accggtgtta acggctgtgc
    12121 cagtaaaaaa gagtggtgtc gttctgccag aggcggactt tgaggcctgg gccacgttac
    12181 aggtagatta ccaatgaaga aaaagcgtac gttgtttttt attagtagcc tgatgttgct
    12241 gggcagtgga accacaatag caggagataa tttgcacttt accggcaatt taatcagtaa
    12301 atcgtgtacg ccagtgatta atggtagcca gttggcagaa gtacattttc ctgccattgc
    12361 cgccagcgat ttaatgaacc ttgggcagtc agagcgagtg ccattggtgt ttcaattgaa
    12421 agattgtcat agttctacat tgtttaatgt caaagtaacg ttgacaggaa cagaagatag
    12481 tgctctaccc ggctttctgg cctttgactc ctcgtcatca gcatccggcg ctgggattgg
    12541 tattgaaact gcggcaggca catcggtgcc aatcaacaat acaacaggcg tcacgctccc
    12601 gctgaatcag ggaaataaca gcctaaattt taatacctgg ttacaggcaa agagcgggcg
    12661 agatgtaacg tccggagact ttagtgccac agtgacggca acctttgagt atttctgaaa
    12721 tgaaaaaatg gaccataatt ttcgcgagcc taatgctgct cgttctgagt gttgtcggcg
    12781 catctaagag ttatgctgga gataaactta tgagtgcgag ttttgacagt gtgagagttt
    12841 attatgccat ggacaaggta accggtgctg tagcgagcag tgttttcaat gttacggtga
    12901 taacgccaaa ggaggttaca tatgggaaat atgattcttt tgcgtataaa ggcgatacgt
    12961 tgcgggttat ttcctggtct gggtcgggtt cagctccaac tttggtactg actgactttg
    13021 atacaattaa taactcaaat tgccctggca tagatacgaa aatttttcgt tgtgcatata
    13081 tgacgtttaa aattaccgtt gcatctgatg attatggttg cccttggatc gcatcctttt
    13141 attcctatac agatttaccc ggatttggaa gctatacggc ccctacagtg cataacacaa
    13201 tatgcccaac gataccggtc acttcatacg atatctcctg gagtgaaaat tatgtcagcc
    13261 ataataaggc gttacgaatt cagtccacgg ggtcaacagt gaccaccacg ctttcgacct
    13321 acctgatgga aggcggcaga ctctgtgatg gcagtaattt ttctgataat gatggcagag
    13381 gagcatactg ccgcgcggta tcagaattgt taacctttac ctcttacggt tgtgataaat
    13441 caacggtaac ggttacgcca actcgtcatc cggtaacgga taaagtacta cacgatattg
    13501 tggtgaatgt gaataccagt agtgggcaac cgattgattc gacgtgtcgt tttcagtatg
    13561 tattgaatga actgtagttc gtagtattgc tctgatgcgt cttatcaggc ctacgaagtg
    13621 aattgcaatt tattgaatta tcacatattt gtaggccgga taaggcgttt acgctgctcc
    13681 ggcatttgct gccagcattc agggcaactc cggcggcaac cactcttcca cttcaatcaa
    13741 caccaacaaa gccgcatcac cgccgtactc tttcggtgcc tggtgaaatg ccatcacatg
    13801 cggatgttgt gccagccaca gcggtgtttg ttgcttcaaa atatgcttcc cgtgcccatg
    13861 catcacgcag gcacaaaaca catgttcgcg gcgacaggcg gcaatcaacg cgcccagttc
    13921 ctgcttggcc tgcagctgcg tcagaccgtg taaatccaaa aacaattccg gtgaataatc
    13981 gccgcggcgc agtttcttcg cctcaaaatg gctgacatcc gggcgaacat atttcaccgg
    14041 accttcggta tttaataacg gctgaaactc atcggagaaa taatggctgg catcagcctg
    14101 ctccgggatc aagcgtttca ccggcacttc gctgattttt ttacgctgcg gtcggtggac
    14161 aatcgtgtcc tgcttaatct tgcgagtccc cgccatcaac tggcgaaaca gagcctggtc
    14221 ctcctcgcta agtgttgttt tctttttcat ttatctgtct ctatttctaa ttttcctaca
    14281 gtttacccga ctcgtagcac aatcgatcgt tttaaacgta tttttcctca ctcaccgcgc
    14341 gtgaatgctg atttatcgtc atcttcatgg caaactagcc gccgaaatta ttgcgatcat
    14401 gccctggagg aatacgtgga taaaattttc gttgatgaag cagtaaatga gctgcaaacc
    14461 attcaggaca tgttgcgctg gtcggtaagc cgcttcagcg cggcaaatat ctggtacggt
    14521 cacggcaccg ataacccgtg ggatgaagcc gtacagctgg tgttaccatc gctctacctg
    14581 ccgctggata tcccggaaga tatgcgcacc gcgcgtctga cctccagcga aaaacaccgt
    14641 attgttgaac gcgtgatccg ccgcgtcaac gagcgcattc cggtggctta cctgaccaac
    14701 aaagcctggt tctgcggtca tgaattttac gtcgatgaac gcgtgctggt gccgcgctcg
    14761 ccgattggtg aactgatcaa caataaattt gccggactca ttagcaagca accgcagcat
    14821 attttagata tgtgtaccgg tagcggctgc atcgccattg cctgtgctta tgccttcccg
    14881 gaagcggaag tcgacgcggt agatatctct cccgacgcgc tggcggttgc cgaacagaac
    14941 atcgaagaac acggtctgat ccacaacgtc attccgattc gttccgatct gttccgtgat
    15001 ttgccgaaag tgcagtacga tctgattgtc actaacccgc cgtatgtcga cgcggaagat
    15061 atgtccgacc tgccaaacga ataccgtcac gagccggaac tgggcctggc atctggcacc
    15121 gacggcctta aactgacgcg tcgcattctt ggaaacgcgg cagattacct tgctgatgat
    15181 ggcgtgttga tttgtgaagt cggcaacagc atggtacatc ttatggaaca atatccggat
    15241 gttccgttca cctggctgga gtttgataac ggcggcgatg gtgtgtttat gctcaccaaa
    15301 gagcagctgc tggcggcccg tgaatatttc gcgatttata aagattaagt aaacacgcaa
    15361 acacaacaat aacggagccg tgatggctgg aaacacaatt ggacaactct ttcgcgtaac
    15421 caccttcggc gaatcgcatg ggctggcgct cggctgcatc gtcgatggtg ttccgccagg
    15481 cattccgctg acggaagcgg acctgcaaca tgacctcgac cgtcgtcgcc ctgggacatc
    15541 gcgctatacc acccagcgcc gcgagccgga tcaggtcaaa attctctccg gtgtttttga
    15601 aggcgttact actggcacca gcattggctt gttgatcgaa aacactgacc agcgctctca
    15661 ggattacagc gcaattaaag acgttttccg cccaggccat gctgattaca cctacgaaca
    15721 aaaatacggt ctgcgcgatt atcgcggcgg tggacgttct tccgcccgcg aaaccgccat
    15781 gcgcgtggcg gcaggggcga ttgccaaaaa atatctcgcc gagaaatttg gtattgaaat
    15841 ccgcggctgc ttgacccaga tgggcgacat tccgctggaa atcaaagact ggtcgctggt
    15901 tgaacaaaat ccgttcttct gcccggaccc ggacaaaatc gacgcgttag atgaactgat
    15961 gcgcgcgctg aaaaaagagg gcgactccat cggcgcgaaa gtcaccgttg ttgccagtgg
    16021 tgtccccgcc ggacttggcg agccggtctt tgaccgcctg gatgccgaca tcgcccatgc
    16081 gctgatgagc atcaacgcgg tgaaaggcgt ggaaattggc gacggttttg acgtagtggc
    16141 gctgcgcggc agccagaacc gcgacgaaat caccaaagac ggtttccaga gcaaccatgc
    16201 gggcggcatt ctcggcggta tcagcagcgg gcagcaaatc attgctcata tggcgctgaa
    16261 accgacctcc agcattaccg tgccggggcg cactattaac cgctttggcg aagaagttga
    16321 gatgatcacc aaaggccgtc acgatccctg tgtcgggatc cgtgcagtgc cgatcgctga
    16381 agcgatgctg gcgatcgttt taatggatca cctgttacgg cagcgggcgc aaaatgccga
    16441 tgtgaagact gatattccac gctggtaaaa aatgaacaaa accgcgattg cgctgctggc
    16501 tctgcttgcc agtagcgcta gcctggcagc gacgccgtgg caaaaaataa cccaacctgt
    16561 gccgggtagc gcacaatcga taggcagttt ttctaatggc tgtattgtcg gcgctgacac
    16621 gttgccgata cagtccgaac attatcaggt catgcgtacc gatcagcgtc gctatttcgg
    16681 tcacccggat ctggtgatgt ttatccagcg tctgagtagc caggtgagca atctgggcat
    16741 gggtacggtg ctgattggcg atatggggat gcccgctggt gggcgtttca acggcggtca
    16801 tgccagccac cagaccggac tggatgtcga tatctttctg caactgccga aaactcgctg
    16861 gacctccgcg cagctcttgc gcccgcaagc actggactta gtttcccgcg acggtaaaca
    16921 cgttgtctcc acgctgtgga agccagaaat tttcagcttg atcaaactcg ctgcccagga
    16981 caaagacgtc acgcgcattt ttgttaatcc ggcgattaaa caacaacttt gccttgatgc
    17041 gggcaccgat cgcgactggt tgcgcaaagt gcgaccctgg ttccagcatc gcgcgcatat
    17101 gcatgtacga ttacgttgcc ctgccgatag tctggagtgt gaagatcaac ctttaccgcc
    17161 atcaggcgat ggttgcgggg cagaactgca aagctggttt gcacctctaa aaccgggaac
    17221 aacaaagcct gagaagaaga caccgcctcc gttgccgcct tcctgccagg cgctactgga
    17281 tgagcacgtg atctaatgga aacgtttaat agcctgttta tggtttcccc gctgttgctg
    17341 ggagttctct tttttgtcgc catgctggcg ggatttatcg actcgattgc cggtggcggt
    17401 gggttactca ccattccggc attgatggct gcgggaatgt ctcccgctaa cgcgctggca
    17461 accaataaac tgcaagcctg cggcggctct atttccgcca ctatctactt tattcgccgc
    17521 aaagtggtta gtttaagcga tcagaaactc aatattgcca tgacctttgt cggctcaatg
    17581 agcggcgcac tgttggtaca gtacgttcag gctgatgtct tacggcagat tttgcccatt
    17641 ctggtgattt gtatcggcct ctatttcttg ctgatgccaa agctgggtga agaagatcgc
    17701 cagcgccgga tgtacggttt acccttcgcg ttaattgccg gtggctgcgt cggtttttat
    17761 gacggattct ttggcccggc tgccggatcg ttttacgcgc tggccttcgt tacgctgtgc
    17821 ggattcaacc tcgccaaagc cacggctcac gccaaattac tcaacgcaac gtcaaacatt
    17881 ggcggtctgc tgctgtttat tctcggcggc aaagtgattt gggcgacggg ctttgtcatg
    17941 ctggtggggc agttcctcgg cgcgcgcatg ggttcacggc tggtgttgag caaaggacaa
    18001 aagctgatcc gcccgatgat tgttatcgtc tcggcggtga tgagtgccaa attactttac
    18061 gatagccacg gacaggagat cctccactgg ttggggatga actaatgaac agtacacacc
    18121 actatgagca gttgattgaa atttttaata gctgctttgc cgatgaattt aatacccgtc
    18181 tgattaaagg cgacgacgaa ccgatctatc ttcctgctga tgcggaagta ccgtataacc
    18241 gaattgtctt tgcccacggc ttttatgcca gcgcaattca tgagatttcg cactggtgta
    18301 ttgccggaaa agcgcgccgt gagctggttg atttcggcta ctggtattgc ccggatgggc
    18361 gtgatgccca aacgcaaagc cagtttgaag atgttgaagt gaagccgcag gcactcgact
    18421 ggttgttctg tgtggcggcg ggatatccgt ttaacgtcag ttgcgacaat ctggaaggcg
    18481 acttcgaacc ggatcgtgtt gtgtttcaac ggcgggtaca tgcgcaggtg atggattatc
    18541 tgacaaatgg cattcccgag cgtccggcac gcttcattaa agcactacaa aattattatc
    18601 acacgccgga acttacggcg gaacagttcc cgtggccgga agcgctcaac tgaagccggt
    18661 gccacgtttt acaatgagga aagaagatga tcgcggagtt tgaatcacgc attctggcat
    18721 taatcgacgg catggttgac catgccagtg atgatgagtt gtttgccagt gggtatttgc
    18781 gtggccacct gacattagcc atcgcagaac tggaaagtgg tgatgaccac tccgctcagg
    18841 cggtgcatac gaccgttagc cagagtctgg aaaaagccat tggtgccggt gaattgtcgc
    18901 cgcgtgacca ggcgctggtg accgatatgt gggaaaacct gtttcagcag gcgtcacagc
    18961 agtaagttgc ggcgtctgga gcaccatgcc tgatgcgacg cttatcgcgt cttatcaggc
    19021 ctacacgtca cacgtaggtc ggataaggcg ttcacgccgc atccggcaaa cactgcgaga
    19081 gcagattacc ccgccttaac cgctttaccc ttcaacaatt tccgcaccca taaccgattc
    19141 gggtttaacg ccgtcagcgt actggcatcc atcggaatcg gttcgtcgct catctgcgcc
    19201 gccagaatct cggcacacag cggggcagaa cacaaaccgc gagaacctaa agccgcaaac
    19261 ataaagagat cgtcaaaaac cggcgcgctt accgcctcat ctttctgctc cgccaacgac
    19321 gcatattcca cgagtgttgc ctcataatcg ggaacattgc ctaccattgg cagatgatcg
    19381 cgggtggcac aacgcacacc gcagcgcgcc tctttatcac tgacatcaac ctcttttgcc
    19441 cactgtgcct gcgggaaaca atcaatcaac cgctggcgat tctgctgctg atcgtcctca
    19501 ctgtacgccg tatcttcgct gccgcgatga taactggcac caatacaatg atgttgattc
    19561 gccggatttt gtggcgtgag ataaccgtca tagcacagca cctgcttcag ctctgccagt
    19621 tccggcgttg tcggaatatg gctgacctgc ccggcaaccg aatacaccgg gagagtcgac
    19681 gtttggctga aacggctgat ttgatgcccg ttcgccagta ccactacgct gtgtgttgct
    19741 tgctgatctc ctgcaaaatt caacaaccaa cagtcatcct tacgggataa attctgtaac
    19801 tgatattgat aataaatctg caaaccctgc tgttgcgcca gttccagcac attacgggtc
    19861 agttctgctg ggcacagcca accaccttgc ggataagtaa tgccgctgca atttgtcgca
    19921 acgcccgtaa tttgttcaac cgcatttgcc tcaacggcta cagccagttc tgcgggtaaa
    19981 tccattgaca acatctgtgc gattttatgc tggcttttct catcccagcc taactgcgtg
    20041 acgccgcacc agtcatgatc aaatttaacg ggtaattggt cgtaaaaccg acgagcaaaa
    20101 gtaaacgcat tagagaaaaa gcggtttagc gcctcatcgt gtttgcttaa taacggatac
    20161 agcgccccct ggcgattgcc ggaagcaccc agcgcggggg cttcatccgc gcaataaagc
    20221 gctacctgcc agccgcgccg taataacgcc agcgacaaca acgcgctggc aataccaccg
    20281 ccgataatcg ctacttcccg tttgctgctg cccgtacgat taaaccacgg cgtggagcag
    20341 gggagtggta atgtctgttc catcacccca caaagcattt cccgtttgcg cccaaagccc
    20401 ttacgttttt gcatcgtgaa tccggcttcc tgcaaaccgc ggcggacaaa accggcagac
    20461 gtaaatgtcg ccagcgtgcc gcctggacgc gccaaccttg ccatggcgtt aaacagattt
    20521 tgcgtccaca tatccgggtt tttagctggc gcaaagccgt ccagaaacca ggcatctact
    20581 ttttgattta gcgaatcgtc cagttggctg gtcagttcgt taatatcgcc aaaccataaa
    20641 tccagcgtca cgcggccttc attgagcaat aaacgatggc aaccgggcaa gggcattggc
    20701 cactgcgcct gaagttgttc tgcccacgga gccagttccg gccagtgttg atgcgctaag
    20761 gctaaatccg cacgggtgag gggaaatttc tcaaaactaa tgaaatgtaa gcgttgtaat
    20821 tgcgcttgcg gatgcgcttc gcgaaactga tcaaatgcct gccatagcgt caggaagttt
    20881 aatccggtgc cgaagccgct ctctgctacc acaaacagag gatgtggatg ctcaggaaag
    20941 cgtacctcta attggttgcc tcccagaaaa acataacgcg tctcttccag cccgttatcg
    21001 ttggaaaaat agacatcgtc aaaatctcgg gaaacaggtg taccctcagc attaaattcg
    21061 aggttggcag gttgtatgga gtagtgtttc acgtaagtta ctcgtcttac aggcggtggc
    21121 tcgatcttag cgatgtgtgt aaggctgcgc aaatttctct attaaatggc tgatcggact
    21181 tgttcggcgt acaagtgtac gctattgtgc attcgaaact tactctatgt gcgacttaca
    21241 gaggtattga atgaaacgtg cagtgattac tggcctgggc attgtttcca gcatcggtaa
    21301 taaccagcag gaagtcctgg catctctgcg tgaaggacgt tcagggatca ctttctctca
    21361 ggagctgaag gattccggca tgcgtagcca cgtctggggc aacgtaaaac tggataccac
    21421 tggcctcatt gaccgcaaag ttgtgcgctt tatgagcgac gcatccattt atgcattcct
    21481 ttctatggag caggcaatcg cagatgcggg cctttctccg gaagcttacc agaataaccc
    21541 gcgcgttggc ctgattgcag gttctggcgg cggttccccg cgtttccagg tattcggcgc
    21601 tgacgcaatg cgcggcccgc gcggcctgaa agcggttggc ccgtatgtgg tcaccaaagc
    21661 gatggcatcc ggcgtttctg cctgcctcgc cacgccgttt aaaattcatg gcgttaacta
    21721 ctccatcagc tccgcgtgtg cgacttccgc acactgtatc ggtaacgcag tagagcagat
    21781 ccaactgggc aaacaggaca tcgtgtttgc tggcggcggc gaagagctgt gctgggaaat
    21841 ggcctgcgaa ttcgacgcga tgggtgcgct gtctactaaa tacaacgaca ccccggaaaa
    21901 agcctcccgt acttacgacg ctcaccgtga cggtttcgtt atcgctggcg gcggcggtat
    21961 ggtagtggtt gaagagctgg aacacgcgct ggcgcgtggt gctcacatct atgctgaaat
    22021 cgttggctac ggcgcaacct ctgatggtgc agacatggtt gctccgtctg gcgaaggcgc
    22081 agtacgttgt atgcagatgg cgatgcatgg cgttgatacc ccaatcgatt acctgaactc
    22141 tcacggtact tcgactccgg ttggcgacgt gaaagagctg gcagctatcc gtgaagtgtt
    22201 cggcgataag agcccggcga tttctgcaac caaagccatg accggtcact cactgggcgc
    22261 tgcgggtgtg caggaagcta tctactctct gctgatgctg gaacacggct ttatcgcccc
    22321 gagcatcaac attgaagagc tggacgagca ggctgcgggt ctgaacatcg tgaccgaaac
    22381 gaccgatcgc gaactgacca ctgttatgtc taacagcttc ggcttcggcg gcaccaacgc
    22441 cacgctggta atgcgcaagc tgaaagatta attcgcagta ggccggagta ggtcggataa
    22501 gacgcgccag cgtcgcatcc gacgttacgc gccaatgcgg cctccggcac taacgcaaaa
    22561 gggaacctga tggttccctt tttcacatca ttgacaatcg ccaccagttc caggcaaact
    22621 tcccgctttg tcgatttcct tctgaaaaga cgtacgcgtt aaatcctgcc aacgcaccgt
    22681 aaccctgaaa ccagagagat gagacgggga tactcctcgc cttgcgctgc attctggagt
    22741 aatgcatgac tgctgtaagc caaaccgaaa cacgatcttc tgccaatttt tcgctcttcc
    22801 gcatcgcttt tgcggttttt ctcacctaca tgaccgtagg gctgccgttg ccggttatcc
    22861 cgctgtttgt tcatcatgaa ctgggctatg gcaataccat ggtcggcatt gccgtcggga
    22921 ttcagtttct ggctacggtg ctgacgcgtg gctatgccgg gcgactggcc gatcaatatg
    22981 gcgcaaaacg ttcggcgctt caggggatgt tagcttgtgg tctggctggc ggcgcgttgc
    23041 tgctggcggc gattttgcct gtctccgcac cgttcaaatt tgccctgttg gtcatcgggc
    23101 gtttgattct tgggtttggt gaaagccagt tactgacagg cgctctgacc tgggggttag
    23161 gcatcgtagg gccaaaacac tctggcaaag tgatgtcatg gaatggaatg gcgatttacg
    23221 gtgccctcgc tgttggtgct ccgcttggcc tgttgattca tagccattac ggttttgccg
    23281 cactggcgat caccacaatg gtattaccct tactggcgtg ggcctgtaac ggcacagtgc
    23341 gcaaagtacc ggccctggcg ggagaacgtc catcgctgtg gagcgttgtc gggtttatct
    23401 ggaaaccagg gttagggctg gcactacaag gcgttggttt tgcggttatc gggactttcg
    23461 tttcgctcta ctttgccagc aaaggatggg cgatggcggg ctttactctt accgagtttg
    23521 gcggcgcatt tgtcgtgatg cgcgtcatgt ttggctggat gccggaccgt tttggcggcg
    23581 tgaaagtggc gattgtctct ctgcttgtag aaacggtggg cttgttgctg ctctggcaag
    23641 ccccaggtgc gtgggtcgca ttagcgggcg cggcgttaac cggagccgga tgttcgctta
    23701 tctttcctgc gctgggcgtg gaggtggtta aacgcgtccc ctcacaagtt cgcggcaccg
    23761 cactgggcgg ttacgccgcg tttcaggata tcgccctcgg cgtctccggg ccgctggcgg
    23821 gaatgctggc gaccacgttt ggttactctt cggtatttct tgccggggcg atctctgcgg
    23881 tgctgggaat tattgtcacg atactgtcat ttcgtcgggg ctaacgaacc agccagacca
    23941 gcatcaggat aatcgcgact aacagtatcc acagcgcggg acgcgttgcc atattttgca
    24001 acgtgtccat catcggtgga aaaggattaa gcagcggttg catatcacgc ggatcaatcc
    24061 ctttaacccg ccgttgataa agctgattaa gaatatcctc ggcctgggca gaagaaagcg
    24121 atgattgcgc agaaagacca tatttttgct ggatatacgc cgataacgct gccagttcac
    24181 tggcatctaa aggttgtttt agcgccatct gtagtgattc cagcgtcggc gtattttgct
    24241 ggcttaacgt ctggcgcgcc tgcaaccagg tcaccagatg gttaaacagt ttcgctggaa
    24301 ttaactcgcc atcgttcacc ccggaaagtt ccagcatcga ttgccagatc tgtttgctgg
    24361 gttcccccgt tgccgccgca agtttggtca ccagctgttt tagcgcattg tgctccgccg
    24421 gtaataaagg acggtcggtc gcctcgcgct gctgtggttg cggaataacc atcttccctt
    24481 cctgcaacag ggtgagaatg gtttttaatt gctccggtga gagctgattc agcggcgtct
    24541 gaccaaagtt atgacggata taatccgtga ccgcctgacg attattcccc agacgtaaat
    24601 actcccctaa ttgcgctaaa agctggcggg cagaatggct tttttgcgcg gccagcagac
    24661 gttgcgccag attatgctca gcggcaggga agtgacgcga aagcagcggt gaatctcccg
    24721 acagaccaat atcgtgcctg atacccgccc acagttctgc tctttgttgt tgcgtcagtg
    24781 aggtcacttt cgtcattaag ctttccagcg aagtacgttg ctgactggat aaaggctgat
    24841 tgcccgcgcc agacggcagg ttatctccct gacctggtgg ttgcccagga ggagggccgg
    24901 aaataggttg tatcattacg tatccttata cctgaaatct tcgcaagtat gcctggccgc
    24961 gagattatgg cacacttgtc cggttaactc tcgtctcata caggtaacac aaacgtgaaa
    25021 atccttgttg atgaaaatat gccttatgcc cgcgacttat ttagccgttt gggtgaggtg
    25081 accgcggttc ccgggcgtcc aatccccgtc gctcaactgg cagacgcgga tgcgctgatg
    25141 gtgcgttcgg tcacgaaagt gaatgaatct ttgctggcag gaaaacccat taaatttgtt
    25201 ggcactgcca ctgcggggac cgaccatgtc gatgaagcat ggttgaagca ggcgggaatt
    25261 ggtttttccg ctgcacctgg ctgtaatgcg attgcggtgg tggaatatgt tttctcctca
    25321 ctactgatgc ttgccgaacg cgatggattt tcactgcacg accgtacggt ggggatcgtg
    25381 ggcgttggta acgttggacg tcgattgcag gcgcgactgg aagcgttagg gattaaaaca
    25441 ttgctttgcg atccgcctcg cgctgaccgt ggggatgagg gtgatttccg ctcgctggat
    25501 gaattagttc agcatgcgga tattctgact ttccatacgc cactcttcaa agatgggccg
    25561 tacaaaacgc tgcatctggc ggatgaaaaa ctgatccgta gcctgaagcc gggagcgatt
    25621 ctgattaacg cctgccgtgg cgcagtcgtc gataatactg cgttgctgac ctgcctgaat
    25681 gaaggccaga agttaagcgt agtgctggat gtctgggaag gcgaaccgga acttaacgtg
    25741 gagttgctga caaaagtgga tatcggcacg ccgcatatcg caggttatac cctggaaggt
    25801 aaagcacgcg gtactacgca agtgtttgaa gcctatagca agtttattgg gcatgaacag
    25861 cacgttgcgc tggatacatt actgcccgcg ccagagtttg gtcgcattac gctgcatggc
    25921 ccgctcgatc aaccgacgct gaaaaggctg gtgcatttgg tgtatgatgt gcgccgcgat
    25981 gacgcaccgc tgcgtaaagt cgccgggata ccgggtgagt tcgataaact gcgcaaaaac
    26041 tatcttgagc gccgtgaatg gtcatctctg tatgtaattt gtgatgacgc cagtgcggca
    26101 tcattgctgt gtaaactggg ttttaacgcc gttcatcatc cggcacgtta atctcttctt
    26161 catgctctct gctgtaacat tggcagggag ctttgctatt tctggagtaa accaccatgt
    26221 ctgaaggctg gaacattgcc gtcctgggcg caactggcgc tgtgggcgaa gccctgcttg
    26281 aaacgctggc tgaacgtcag ttcccggttg gggaaattta tgcactggca cgtaacgaaa
    26341 gcgcaggcga acaactgcgc tttggtggta aaacaatcac tgtgcaggat gccgctgaat
    26401 tcgactggac gcaggcgcag ctggcatttt ttgtcgcagg caaagaagct accgctgcct
    26461 gggttgaaga agcgaccaac tcaggttgcc tggtgatcga cagcagcgga ctgtttgctc
    26521 tcgaacccga cgtaccgctg gtggtgccgg aagtaaaccc gtttgtactg accgattacc
    26581 gcaaccggaa tgtcatcgcc gtaccagaca gtctgaccag ccagctgctg gcggcactga
    26641 aaccgttaat cgatcagggc ggtttgtcgc gtatcagtgt taccagcctg atttcagcct
    26701 ccgcccaggg caaaaaagcg gtcgatgcgt tagcggggca gagtgcgaaa ttgctcaacg
    26761 gcattccgat tgacgaagaa gatttcttcg gtcgtcaact ggcgttcaat atgctgccgt
    26821 tgttgccgga tagcgaaggt agcgtgcgtg aagaacgtcg tatcgttgac gaagtacgca
    26881 aaatcctgca ggacgaaggg ctgatgattt cggcaagcgt cgtccaggca ccggtattct
    26941 acggtcatgc ccagatggtc aactttgaag cactgcgtcc gctggcggca gaagaagcgc
    27001 gtgatgcgtt tgctcagggc gaagatattg tgctctctga agagaacgaa ttcccgactc
    27061 aggtagggga tgcttcgggt acgccgcatc tttctgttgg ctgcgtgcgt aatgactacg
    27121 gtatgccgga gcaagtccag ttctggtcgg tggccgataa cgttcgcttt ggcggcgcgc
    27181 tgatggcagt aaaaatcgcc gagaaactgg tgcaggagta tctgtactaa tgtccgacca
    27241 gcaacaactg ccagtttata aaattgcgct gggcattgag tacgacggca gtaagtatta
    27301 cggctggcaa cggcagaatg aagtccgcag tgtgcaggag aagctggaaa aggcgctctc
    27361 ccaggtggcg aacgaaccca tcaccgtctt ctgcgccggg cgtactgacg caggggtaca
    27421 cggtaccggg caggtggtgc atttcgaaac aaccgcgcag cgcaaagacg cggcgtggac
    27481 cttgggcgta aatgcgaatt tacctggtga catcgccgtg cgttgggtta aagctgtacc
    27541 tgatgatttt catgcccgat ttagcgccac ggctcgccgt tatcgctaca tcatctacaa
    27601 tcatcggctg cgcccggcgg tattgagtaa aggggtaacc catttttacg aaccgctgga
    27661 cgctgaacgg atgcatcggg ctgcgcaatg cttgctgggc gagaatgatt tcacctcgtt
    27721 ccgtgcggtg cagtgccagt cccgaacccc gtggcgcaac gttatgcata ttaatgtcac
    27781 gcgtcacggt ccttatgtgg tggtagatat caaagcgaat gcctttgtac atcatatggt
    27841 caggaatatt gtcggcagcc tgatggaagt cggtgcccac aaccagccgg agagatggat
    27901 agcagagttg ctggcggcaa aggacagaac gctggcggca gcaacggcaa aagcggaagg
    27961 gctgtatctg gtcgcggtgg attaccctga ccggtatgat cttccaaaac cgccaatggg
    28021 cccgctattt ctggcggact aacgagaata atgctcggaa catttcaggc tgtagccaac
    28081 gtagttacag cctgaaagat gacgagtaca aggcataggc aaatatggac ctgatttatt
    28141 tcctcatcga ttttatcctg cacattgacg tgcatctggc agagctggtc gcggagtacg
    28201 gcgtctgggt ttatgccatt ctgtttttga ttctgttctg cgaaacgggg ctggtggtaa
    28261 cgccattcct gcctggtgat tcgctgctgt ttgttgctgg tgcattggcg tcgctggaaa
    28321 ctaacgatct caatgtccat atgatggtgg tgcttatgtt gattgccgct attgtcggtg
    28381 atgcagtgaa ctataccatt ggacggttat ttggtgacaa actgttcagt aacccggatt
    28441 cgaaaatctt tcgtcgcagt tatcttgata agactcatca gttttacgag agacatggcg
    28501 gcaaaacgat tattctcgcc cgttttgtac cgatcgtcag aacgttcgcg ccgtttgttg
    28561 cgggaatggg ccacatgtcg taccgtcatt tcgccgctta taacgtgatc ggcgcactgt
    28621 tgtgggtact gatttttacc tacgcgggtt atttcttcgg cacgctcccc ttcattcaga
    28681 gcaaccttaa gctgatgatt gttggcatta tttttgtttc aatattgcct ggggttttcg
    28741 aatttattcg tcacaaacgc gctgcggcac gcgccgcaaa ataaaaagta actccgcggt
    28801 tcgaccactt ttttatccaa agtttcgggc tgttatgttt taatgtgcaa cattcatggt
    28861 ctgttggggg caaaaatggc attatgcgtc cccaaagata aaactggcat cgaaccaggt
    28921 tcagacagaa aggtccctaa tgagctggat tgaacgaatt aaaagcaaca ttactcccac
    28981 ccgcaaggcg agcattcctg aaggggtgtg gactaagtgt gatagctgcg gtcaggtttt
    29041 ataccgcgct gagctggaac gtaatcttga ggtctgtccg aagtgtgacc atcacatgcg
    29101 tatgacagcg cgtaatcgcc tgcatagcct gttagatgaa ggaagccttg tggagctggg
    29161 tagcgagctt gagccgaaag atgtgctgaa gtttcgtgac tccaagaagt ataaagaccg
    29221 tctggcttct gcgcagaaag aaaccggcga aaaagatgcg ctggtggtga tgaaaggcac
    29281 tctgtatgga atgccggttg tcgctgcggc attcgagttc gcctttatgg gcggttcaat
    29341 ggggtctgtt gtgggtgcac gtttcgtgcg tgccgttgag caggcgctgg aagataactg
    29401 cccgctgatc tgcttctccg cctctggtgg cgcacgtatg caggaagcac tgatgtcgct
    29461 gatgcagatg gcgaaaacct ctgcggcact ggcaaaaatg caggagcgcg gcttgccgta
    29521 catctccgtg ctgaccgacc cgacgatggg cggtgtttct gcaagtttcg ccatgctggg
    29581 cgatctcaac atcgctgaac cgaaagcgtt aattggcttt gccggtccgc gtgttatcga
    29641 acagaccgtt cgcgaaaaac tgccgccagg attccagcgc agtgaattcc tgatcgagaa
    29701 aggcgccatc gacatgatcg tccatcgtcc ggaaatgcgc ctgaaactgg cgagcattct
    29761 ggcgaagttg atgaatctgc cagcgccgaa tcctgaagcg ccgcgtgaag gcgtagtggt
    29821 acccccggta ccggatcagg aacctgaggc ctgataactg ataagggcag ggccactggc
    29881 tctgcccttt tgctattctc accgtaacga atcagcggat accatgatta tcaaacgcac
    29941 tcctcaagcc gcgtcgcctc tggcttcgtg gctttcttat ctggaaaacc tgcacagtaa
    30001 aactatcgat ctcggccttg agcgcgtgag ccaggtcgcg gcgcgtcttg gcgtcctgaa
    30061 accagcgcca tttgtgttta ccgttgcggg tacgaatggc aaaggcacca cctgccgtac
    30121 gctggagtcg attctgatgg cggcagggta caaagtgggc gtctacagtt caccgcatct
    30181 ggtgcgttat accgagcgcg tacgtgtgca ggggcaggaa ttgccggaat cggcccacac
    30241 cgcctctttt gcggagattg aatcggcacg cggtgatatt tccctgacct atttcgagta
    30301 cggtacgctg tcggcgttgt ggctgttcaa acaggcacaa cttgacgtag tgattctgga
    30361 agtagggctg ggcggtcgtc tggacgcaac caatattgtc gatgccgatg tggctgtagt
    30421 aaccagcatt gcgctggatc ataccgactg gctgggacca gatcgcgaaa gtattggtcg
    30481 cgagaaagca ggcatctttc gcagcgcaaa accggcaatt gtcggtgagc cggaaatgcc
    30541 ttctaccatt gctgatgtgg cgcaggaaaa aggtgcactg ttacaacgtc ggggcgttga
    30601 gtggaactat tccgtcaccg atcatgactg ggcgtttagc gatgctcacg gcacgctgga
    30661 aaatctgccg ttgccgcttg tcccgcaacc gaatgccgca acagcgctgg cggcactgcg
    30721 tgccagcggg ctggaagtca gtgaaaatgc cattcgcgac gggattgcca gcgcaatttt
    30781 gccgggacgt ttccagattg tgagcgagtc gccacgcgtt atttttgatg tcgcgcataa
    30841 tccacatgcg gcggaatatc tcaccgggcg tatgaaagcg ctaccgaaaa acgggcgcgt
    30901 gctggcggtt atcggtatgc tacatgataa agatattgcc ggaactctgg cctggttgaa
    30961 aagcgtggtt gatgactggt attgtgcgcc actggaaggg ccgcgcggtg ccacggcaga
    31021 acaactgctt gagcatttgg gtaacggcaa atcatttgat agcgttgcgc aggcatggga
    31081 tgccgcaatg gcggacgcta aagcggaaga caccgtgctg gtgtgtggtt ctttccacac
    31141 ggtcgcacat gtcatggaag tgattgacgc gaggagaagc ggtggcaagt aagtttcaga
    31201 atcggttagt gggcacgatc gtgctggtgg cgctgggggt gattgtactt ccagggctgc
    31261 tggacgggca gaaaaaacat tatcaggatg agtttgcggc tatcccgctg gttccaaaag
    31321 cgggcgatcg cgatgagcct gatatgatgc cagccgccac ccaggcgttg ccgacgcagc
    31381 cgccggaagg cgcagcggaa gaggtgcggg caggtgatgc cgcagcaccg tcgctcgatc
    31441 cggccactat tgcagccaat aacaccgagt ttgaaccgga acctgcaccg gtcgccccac
    31501 cgaagccgaa accggtggag ccgcctaaac cgaaggttga agcaccacct gcgccgaagc
    31561 cagaaccgaa gccggtcgtg gaagaaaaag cagcgccaac gggtaaagcc tatgttgtgc
    31621 aactgggtgc gctgaaaaat gccgataaag tgaatgagat tgtcggtaag ctgcgcggtg
    31681 ccggttatcg ggtttatacg tcgccatcca cgccagtgca gggtaaaatt acccgtattc
    31741 tggttgggcc ggatgcctcg aaagataagc tgaaaggttc gctgggtgag ttgaagcaac
    31801 tttctggctt aagtggcgtg gtaatgggct atacgccgaa ttaatacagt cttgcctgat
    31861 gcgacgctgg cgcgtcttat caggcctggg cagtgttaga accgtaggtc ggataaggcg
    31921 tttacgccgc atccgacacg cattgcacga tgccgcaaag gcataaaaag tcgatggcgt
    31981 tgaatatttt ttcagcgcca tttttattga tgcgtgggaa ggaaatccct acgcaaacgt
    32041 tttctttttc tgttagaatg cgccccgaac aggatgacag ggcgtaaaat cgtgggacac
    32101 atatggtctg gattgattac gccataatcg cggtgattgc tttttcctct ctggttagcc
    32161 tgatccgcgg ctttgttcgt gaagcgttat cgctggtgac atggggttgt gctttctttg
    32221 ttgccagtca ttactacact tacctgtcag tctggtttac gggctttgaa gacgaactgg
    32281 ttcgaaatgg aattgccatc gcggtactgt ttatcgctac cctgattgtt ggtgctatcg
    32341 tgaacttcgt gataggccag ttggtggaga aaacggggtt gtcaggcacc gatcgggtgc
    32401 tgggcgtctg tttcggtgcg ttgcgcggtg tgttgattgt tgccgccatt ctcttctttc
    32461 tcgactcctt taccggggtg tcgaaaagcg aagactggag caaatcacag ctgatcccgc
    32521 agttcagttt tatcatcaga tggttttttg attatctgca aagctcgtca agtttcttgc
    32581 ccagagcgta agtgctctga gatgtggctt aacgaggaaa aagacgtatg tgcggtattg
    32641 tcggtatcgc cggtgttatg ccggttaacc agtcgattta tgatgcctta acggtgcttc
    32701 agcatcgcgg tcaggatgcc gccggcatca tcaccataga tgccaataac tgcttccgtt
    32761 tgcgtaaagc gaacgggctg gtgagcgatg tatttgaagc tcgccatatg cagcgtttgc
    32821 agggcaatat gggcattggt catgtgcgtt accctacggc tggcagctcc agcgcctctg
    32881 aagcgcagcc gttttacgtt aactccccgt atggcattac gcttgcccac aacggcaatc
    32941 tgaccaacgc tcacgagttg cgtaaaaaac tgtttgaaga aaaacgccgc cacatcaaca
    33001 ccacttccga ctcggaaatt ctgcttaata tcttcgctag cgagctggac aacttccgcc
    33061 actacccgct ggaagccgac aatattttcg ctgccattgc tgccacaaac cgcttaatcc
    33121 gcggcgcata tgcctgtgtg gcgatgatta tcggccacgg tatggttgct ttccgcgatc
    33181 caaacgggat tcgtccgctg gtactgggaa aacgtgatat tgacgagaac cgtacagaat
    33241 atatggtcgc ttccgaaagc gtagcgctcg atacgctggg ctttgatttc ctgcgtgacg
    33301 tcgcgccggg cgaagcgatt tacatcactg aagaagggca gttgtttacc cgtcaatgtg
    33361 ctgacaatcc ggtcagcaat ccgtgcctgt ttgagtatgt atactttgct cgcccggact
    33421 cgttcatcga caaaatttcc gtttacagcg cgcgtgtgaa tatgggcacg aaactgggcg
    33481 agaaaattgc ccgcgaatgg gaagatctgg atatcgacgt ggtgatcccg atcccagaaa
    33541 cctcgtgtga tatcgcgctg gaaattgctc gtattctggg caaaccgtac cgccagggct
    33601 tcgttaaaaa ccgctatgtt ggccgcacct ttatcatgcc gggccagcag ctgcgtcgta
    33661 agtccgtgcg ccgtaaactg aatgccaacc gcgccgagtt ccgcgataaa aacgtcctgc
    33721 tggtcgacga ctccatcgtc cgtggcacca cttctgagca gattatcgag atggcacgcg
    33781 aagccggagc gaagaaagtg tacctcgctt ctgcggcacc ggaaattcgc ttcccgaacg
    33841 tttatggtat tgatatgccg agcgccacgg aactgatcgc tcacggtcgc gaagttgatg
    33901 aaattcgcca gatcatcggt gctgacgggt tgattttcca ggatctgaac gatctgatcg
    33961 acgccgttcg cgctgaaaat ccggatatcc agcagtttga atgctcggtg ttcaacggcg
    34021 tctacgtcac caaagatgtt gatcagggct acctcgattt cctcgatacg ttacgtaatg
    34081 atgacgccaa agcagtgcaa cgtcagaacg aagtggaaaa tctcgaaatg cataacgaag
    34141 gatgatgcct tcgctgaggg tgccggtctg gcaccctgac ttgcaactcc cgccgaaata
    34201 ctgcaaagtc tgcctgcaag tctgacaggg caactattta tgaaacgact cattgtaggc
    34261 atcagcggtg ccagcggcgc gatttatggc gtgcgcttat tacaggttct gcgcgatgtc
    34321 acagatatcg aaacgcatct ggtgatgagc caggcggcgc gccagacctt atccctcgaa
    34381 acggattttt ccctgcgcga agtgcaggca ttagctgatg tcacgcacga tgcgcgcgat
    34441 atttccgcca gcatctcttc cggttctttc cagacgctgg ggatggtgat tttaccctgt
    34501 tcaatcaaaa ccctttccgg cattgtccat agctatactg atggcttact gacccgtgcg
    34561 gcagatgtgg tgctgaaaga gcgtcgcccg ttggtgctct gcgtgcgtga aacaccattg
    34621 cacttaggcc atctgcgttt aatgactcag gcggcagaaa tcggtgcggt gattatgcct
    34681 cccgttccgg cgttttatca tcgcccgcaa tcccttgatg atgtgataaa tcagacggtt
    34741 aatcgtgttc ttgaccagtt tgcaataacc cttcctgaag atctctttgc ccgctggcag
    34801 ggcgcataac tccgctgttg ccctgtttca gggcaatttt gcaaccgcga tcaaatcctc
    34861 gacattttgt ttctgccatt caatcgaaac gctgcgattc aaccgctata cctgctatct
    34921 tcaacttcag gacaataatg caacgtctta ttaacatatt taacgttgaa tgttactgtt
    34981 gtcgtcaaga tggcataaga cctgcatgaa agagcctgca aacacacaac acaatacaca
    35041 acataaaaaa gccattttca cttgagggtt atgtatgaag aagtcgattc tcgctctgtc
    35101 tttgttagtc ggtctctccg cagcggcttc cagctatgcg gcgctaccgg agacggtacg
    35161 tatcggaacc gataccacct atgcgccgtt ctcgtcgaaa gatgctaaag gtgattttgt
    35221 tggctttgat atcgatctcg gtaacgagat gtgcaaacgg atgcaggtga aatgtacctg
    35281 ggttgccagt gactttgacg cgctgatccc ctcactgaaa gcgaaaaaaa tcgacgccat
    35341 tatttcgtcg ctttccatta ccgataaacg tcagcaggag attgccttct ccgacaagct
    35401 gtacgccgca gattctcgtt tgattgctgc caaaggttca ccgattcagc caacgctgga
    35461 ttcactgaaa ggtaaacatg ttggtgtgct gcagggatca acccaggaag cttacgctaa
    35521 cgagacctgg cgtagcaaag gcgtggatgt ggtggcctat gccaaccagg atttggtcta
    35581 ttccgatctg gctgcaggac gtctggatgc tgcgttacaa gatgaagttg ctgccagcga
    35641 aggattcctc aagcaacctg ctggtaaagc tttcgccttt gctggctcat cagtaaaaga
    35701 caaaaaatac ttcggtgatg gcaccggtgt agggctacgt aaagatgatg ctgaactgac
    35761 ggctgccttc aataaggcgc ttggcgagct gcgtcaggac ggcacctacg acaagatggc
    35821 gaaaaagtat ttcgacttta atgtctacgg tgactgatac gtcgctggga agctgtacct
    35881 gatggaatga tcatcatggt gcacgccagg tttgttgcac tatcgtggtg cattgaaatg
    35941 catacttaag catttttaat gaaaaataat acgtctaacg gggggaatat tttgccttga
    36001 tggtcaattt tatggcacga taagtgtaac aaacctgtaa atattcccta taaaaagact
    36061 gtcagttgag gacattatga aaaaactggt gctatcgctc tctctggttc tggccttctc
    36121 cagcgcaact gcggcatttg ctgcgattcc gcaaaacatc cgcatcggta ccgacccgac
    36181 ctatgcgcca tttgaatcaa aaaattcaca aggcgaactg gttggcttcg atatcgatct
    36241 ggcaaaggaa ttatgcaaac gcatcaatac gcaatgtacg tttgtcgaaa atccgctgga
    36301 tgcgttaatc ccgtccttaa aagcgaagaa gattgacgcc atcatgtcat cgctttccat
    36361 tacggaaaaa cgtcagcaag aaatagcctt caccgacaaa ctgtacgctg ccgattctcg
    36421 tttggtggtg gcgaaaaatt ctgacattca gccgacagtc gagtcgctga aaggcaaacg
    36481 ggtaggcgta ttgcagggca ccacccagga gacgttcggt aatgaacatt gggcaccaaa
    36541 aggcattgaa atcgtctcgt atcaggggca ggacaacatt tattctgacc tgactgccgg
    36601 acgtattgat gccgcgttcc aggatgaggt cgctgccagc gaaggtttcc tcaaacaacc
    36661 tgtcggtaaa gattacaaat tcggtggccc gtctgttaaa gatgaaaaac tgtttggcgt
    36721 agggaccggc atgggcctgc gtaaagaaga taacgaactg cgcgaagcac tgaacaaagc
    36781 ctttgccgaa atgcgcgctg acggtactta cgagaaatta gcgaaaaagt acttcgattt
    36841 tgatgtttat ggtggctaat cgccatcggt gaaagaaagc ccgctccctt cgggtaaccg
    36901 gagggagaca agaggcggta attcaccaca cacgacagga caggcagcat gttgtatggg
    36961 ttttcaggtg ttattttaca gggtgcgctc gtcacgctgg agctggctat cagctctgta
    37021 gtgctcgctg taatcatcgg tttaattggc gctggcggta agctctcgca aaatcggctt
    37081 tcggggctga ttttcgaagg gtacaccacg ctgattcgtg gcgtgccgga cttagtgttg
    37141 atgctgctga ttttctacgg tttgcagatt gcgctaaaca cggtgacgga ggcgatgggc
    37201 gtcgggcaga ttgatatcga tccgatggtc gctggtatta tcactctcgg ttttatctac
    37261 ggtgcttatt ttaccgaaac gttccgtggt gcttttatgg cagtgccgaa aggacatata
    37321 gaggcggcga cggcgttcgg ttttactcgt gggcaagtgt ttcggcggat catgtttccg
    37381 gcgatgatgc gttacgccct gccaggcatt ggcaacaact ggcaggtgat cctcaaatct
    37441 accgcactgg tttcgttact cggcctggaa gatgtggtca aagccaccca actggcaggc
    37501 aaaagtacct gggaaccgtt ctatttcgcc atcgtctgtg gcgtgattta cctggttttc
    37561 accactgttt ccaatggtgt gctgctgttc cttgagcgcc gctactccgt gggtgtgaag
    37621 agggctgacc tgtgatcgaa atcttacatg aatactggaa accgctgctg tggaccgacg
    37681 gttatcgctt tactggtgtg gcgatcactc tgtggctgct tattttgtcg gtagtgatag
    37741 gcggagtcct ggcgctgttt ctggcgattg gtcgtgtctc cagtaataaa tacatccagt
    37801 ttccaatctg gttatttacc tatatttttc gcggtacgcc gctgtatgtt cagttgctgg
    37861 tgttctattc cggcatgtac acgcttgaga ttgttaaggg aaccgaattc cttaacgctt
    37921 tcttccgcag tggcctgaac tgtaccgtgc tggcgctgac gcttaacacc tgcgcttaca
    37981 ctaccgagat ttttgctggg gcaatccgtt cggttccgca tggggaaatt gaagccgcca
    38041 gagcctatgg cttctcgact tttaaaatgt atcgttgcat tattttgcct tctgcgctgc
    38101 gtattgcgtt accggcatac agcaacgaag tgatcctgat gctgcactct actgcgctgg
    38161 cgtttacggc cactgtgccg gatctgctga aaatagcccg cgatattaac gccgccacgt
    38221 atcaaccttt taccgccttc ggcattgccg cggtgctcta tttaatcatc tcttatgtcc
    38281 tgatcagcct ctttcgcaga gcggaaaaac gctggttgca gcatgtgaaa ccttcttcaa
    38341 cgcactgaga atacgatgtc cgagaataaa ttaaacgtta tcgatttgca caaacgctac
    38401 ggcgaacatg aagtgctgaa aggggtctca ctgcaagcga atgccggaga tgtaataagc
    38461 atcatcggat cgtcgggatc ggggaaaagt acctttctgc gctgcattaa cttcctcgaa
    38521 aaaccgagtg aagggtcgat cgtggtcaat ggccagacga tcaatctggt gcgcgacaaa
    38581 gacggtcaac tcaaagtcgc cgataaaaat caactgcgct tactgcgcac acgcctgacg
    38641 atggtattcc agcacttcaa tctctggagc catatgacgg tgctggaaaa cgtcatggaa
    38701 gcgccgattc aggtgttggg cctgagcaag caggaagcgc gcgagcgggc ggtgaagtat
    38761 ctggcgaaag tcgggataga cgaacgtgcg caggggaaat atccggtgca tctttccggc
    38821 ggtcagcaac agcgtgtttc tatcgcgcgg gcgctggcga tggaaccgga agttttactg
    38881 tttgatgaac ctacctcggc gctcgatcct gaactggtag gcgaagtgtt acgtattatg
    38941 cagcaactgg cagaagaggg gaaaaccatg gtggtagtga ctcacgaaat gggctttgct
    39001 cgccatgttt ctactcacgt cattttcctc catcagggga aaatagaaga agagggcgcg
    39061 ccggagcagt tatttggcaa cccgcaaagc cctcgtctgc aacagttcct taagggatcg
    39121 ctgaaataat ttcctctccg gtggctaacg ctgccggagt attttcatgg ggaatttgcg
    39181 agtcagcttc cgctgaaatg taaaagttgc agtccgctaa aaatacttct gaattcgctg
    39241 tccaaagata aaaactgcct gctgcattgg gtgttaatcg tggttattgt cgcgtcatct
    39301 taaatttgag gggtgacgga acgccatgac aatatcgaca acttccacgc cgcatgatgc
    39361 ggtatttaaa tcttttttac gccatccag
//
LOCUS       DAJVXP010000023        38337 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVXP010000023 DAJVXP010000000
VERSION     DAJVXP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.023"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            59..1423
                     /locus_tag="OQS10_001167"
     CDS             59..1423
                     /locus_tag="OQS10_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292249.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OQS10_001168"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OQS10_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS2292250.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="OQS10_001169"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="OQS10_001169"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS2292251.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="OQS10_001170"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="OQS10_001170"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS2292252.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OQS10_001171"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OQS10_001171"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS2292253.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="OQS10_001172"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="OQS10_001172"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS2292254.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="OQS10_001173"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="OQS10_001173"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS2292255.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="OQS10_001174"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="OQS10_001174"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS2292256.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="OQS10_001175"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="OQS10_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS2292257.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OQS10_001176"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OQS10_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS2292258.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OQS10_001177"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OQS10_001177"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS2292259.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OQS10_001178"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OQS10_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS2292260.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OQS10_001179"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OQS10_001179"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS2292261.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OQS10_001180"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OQS10_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS2292262.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OQS10_001181"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OQS10_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS2292263.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="OQS10_001182"
     CDS             13749..14288
                     /locus_tag="OQS10_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS2292264.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="OQS10_001183"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="OQS10_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS2292265.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="OQS10_001184"
     CDS             complement(15648..15995)
                     /locus_tag="OQS10_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS2292266.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OQS10_001185"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OQS10_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS2292267.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OQS10_001186"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OQS10_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS2292268.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OQS10_001187"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OQS10_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS2292269.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OQS10_001188"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OQS10_001188"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS2292270.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OQS10_001189"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OQS10_001189"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS2292271.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="OQS10_001190"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="OQS10_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS2292272.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OQS10_001191"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OQS10_001191"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS2292273.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OQS10_001192"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OQS10_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS2292274.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OQS10_001193"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OQS10_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS2292275.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OQS10_001194"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OQS10_001194"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS2292276.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OQS10_001195"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OQS10_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS2292277.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="OQS10_001196"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="OQS10_001196"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS2292278.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="OQS10_001197"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="OQS10_001197"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS2292279.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="OQS10_001198"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="OQS10_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS2292280.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OQS10_001199"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OQS10_001199"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS2292281.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="OQS10_001200"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="OQS10_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS2292282.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="OQS10_001201"
     CDS             complement(37465..37920)
                     /locus_tag="OQS10_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292283.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="OQS10_001202"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="OQS10_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAJVXP010000024        37967 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVXP010000024 DAJVXP010000000
VERSION     DAJVXP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.024"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQS10_001203"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OQS10_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS2292284.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQS10_001204"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OQS10_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS2292285.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OQS10_001205"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OQS10_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS2292286.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OQS10_001206"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OQS10_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS2292287.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OQS10_001207"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OQS10_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS2292288.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OQS10_001208"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OQS10_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS2292289.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OQS10_001209"
     CDS             complement(6574..7338)
                     /locus_tag="OQS10_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2292290.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQS10_001210"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OQS10_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS2292291.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OQS10_001211"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OQS10_001211"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS2292292.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OQS10_001212"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OQS10_001212"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS2292293.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OQS10_001213"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OQS10_001213"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS2292294.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OQS10_001214"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OQS10_001214"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS2292295.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OQS10_001215"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OQS10_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS2292296.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OQS10_001216"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OQS10_001216"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS2292297.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQS10_001217"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OQS10_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS2292298.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OQS10_001218"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OQS10_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS2292299.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OQS10_001219"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OQS10_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS2292300.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OQS10_001220"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OQS10_001220"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS2292301.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OQS10_001221"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OQS10_001221"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS2292302.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OQS10_001222"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OQS10_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS2292303.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OQS10_001223"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OQS10_001223"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS2292304.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQS10_001224"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OQS10_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS2292305.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQS10_001225"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OQS10_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS2292306.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQS10_001226"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OQS10_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS2292307.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OQS10_001227"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OQS10_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQS10_001228"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OQS10_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS2292308.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OQS10_001229"
     CDS             complement(31717..33003)
                     /locus_tag="OQS10_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2292309.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OQS10_001230"
     CDS             complement(33054..33995)
                     /locus_tag="OQS10_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS2292310.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OQS10_001231"
     CDS             complement(34010..34780)
                     /locus_tag="OQS10_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS2292311.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="OQS10_001232"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="OQS10_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS2292312.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="OQS10_001233"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="OQS10_001233"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS2292313.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
BASE COUNT         9062 a        10441 c         9952 g         8512 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgcta
//
LOCUS       DAJVXP010000025        37768 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVXP010000025 DAJVXP010000000
VERSION     DAJVXP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.025"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OQS10_001234"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OQS10_001234"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS2292314.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OQS10_001235"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OQS10_001235"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS2292315.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OQS10_001236"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OQS10_001236"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS2292316.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OQS10_001237"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OQS10_001237"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS2292317.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OQS10_001238"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OQS10_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS2292318.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OQS10_001239"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OQS10_001239"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS2292319.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OQS10_001240"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OQS10_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS2292320.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OQS10_001241"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OQS10_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS2292321.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OQS10_001242"
     CDS             6537..6773
                     /locus_tag="OQS10_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS2292322.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OQS10_001243"
     CDS             6776..7633
                     /locus_tag="OQS10_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS2292323.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OQS10_001244"
     CDS             7654..9645
                     /locus_tag="OQS10_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS2292324.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OQS10_001245"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OQS10_001245"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS2292325.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OQS10_001246"
     CDS             complement(10248..11144)
                     /locus_tag="OQS10_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS2292326.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OQS10_001247"
     CDS             complement(11193..11432)
                     /locus_tag="OQS10_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS2292327.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OQS10_001248"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OQS10_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS2292328.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OQS10_001249"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OQS10_001249"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS2292329.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OQS10_001250"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OQS10_001250"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS2292330.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OQS10_001251"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OQS10_001251"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS2292331.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OQS10_001252"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OQS10_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OQS10_001253"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OQS10_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS2292332.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OQS10_001254"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OQS10_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS2292333.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OQS10_001255"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OQS10_001255"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS2292334.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OQS10_001256"
     CDS             complement(21570..22739)
                     /locus_tag="OQS10_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292335.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OQS10_001257"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OQS10_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS2292336.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OQS10_001258"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OQS10_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS2292337.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OQS10_001259"
     CDS             complement(24315..25247)
                     /locus_tag="OQS10_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2292338.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OQS10_001260"
     CDS             25360..26571
                     /locus_tag="OQS10_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS2292339.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OQS10_001261"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OQS10_001261"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS2292340.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OQS10_001262"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OQS10_001262"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS2292341.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OQS10_001263"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OQS10_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS2292342.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OQS10_001264"
     CDS             complement(30320..31576)
                     /locus_tag="OQS10_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS2292343.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OQS10_001265"
     tRNA            31884..31960
                     /locus_tag="OQS10_001265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OQS10_001266"
     tRNA            31965..32041
                     /locus_tag="OQS10_001266"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OQS10_001267"
     CDS             32149..32454
                     /locus_tag="OQS10_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS2292344.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OQS10_001268"
     CDS             32580..34184
                     /locus_tag="OQS10_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS2292345.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OQS10_001269"
     CDS             complement(34196..35008)
                     /locus_tag="OQS10_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292346.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OQS10_001270"
     CDS             complement(35012..35797)
                     /locus_tag="OQS10_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS2292347.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OQS10_001271"
     CDS             complement(35794..36462)
                     /locus_tag="OQS10_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS2292348.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OQS10_001272"
     CDS             complement(36526..37164)
                     /locus_tag="OQS10_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS2292349.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37768)
                     /locus_tag="OQS10_001273"
     CDS             complement(37177..>37768)
                     /locus_tag="OQS10_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS2292350.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9022 a         9930 c         9381 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccg
//
LOCUS       DAJVXP010000026        36523 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVXP010000026 DAJVXP010000000
VERSION     DAJVXP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.026"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(18..929)
                     /gene="gsiD"
                     /locus_tag="OQS10_001274"
     CDS             complement(18..929)
                     /gene="gsiD"
                     /locus_tag="OQS10_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS2292351.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(932..1852)
                     /gene="gsiC"
                     /locus_tag="OQS10_001275"
     CDS             complement(932..1852)
                     /gene="gsiC"
                     /locus_tag="OQS10_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS2292352.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1870..3408)
                     /gene="gsiB"
                     /locus_tag="OQS10_001276"
     CDS             complement(1870..3408)
                     /gene="gsiB"
                     /locus_tag="OQS10_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS2292353.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3428..5299)
                     /gene="gsiA"
                     /locus_tag="OQS10_001277"
     CDS             complement(3428..5299)
                     /gene="gsiA"
                     /locus_tag="OQS10_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS2292354.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5286..6251)
                     /gene="iaaA"
                     /locus_tag="OQS10_001278"
     CDS             complement(5286..6251)
                     /gene="iaaA"
                     /locus_tag="OQS10_001278"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS2292355.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6455..7690
                     /gene="moeA"
                     /locus_tag="OQS10_001279"
     CDS             6455..7690
                     /gene="moeA"
                     /locus_tag="OQS10_001279"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS2292356.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7690..8439
                     /gene="moeB"
                     /locus_tag="OQS10_001280"
     CDS             7690..8439
                     /gene="moeB"
                     /locus_tag="OQS10_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS2292357.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8618..9280)
                     /gene="fsa"
                     /locus_tag="OQS10_001281"
     CDS             complement(8618..9280)
                     /gene="fsa"
                     /locus_tag="OQS10_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS2292358.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9411..10310
                     /locus_tag="OQS10_001282"
     CDS             9411..10310
                     /locus_tag="OQS10_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS2292359.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10316..12748
                     /locus_tag="OQS10_001283"
     CDS             10316..12748
                     /locus_tag="OQS10_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS2292360.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12894..13709
                     /gene="ybiV"
                     /locus_tag="OQS10_001284"
     CDS             12894..13709
                     /gene="ybiV"
                     /locus_tag="OQS10_001284"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS2292361.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13861..15126
                     /locus_tag="OQS10_001285"
     CDS             13861..15126
                     /locus_tag="OQS10_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS2292362.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15093..16253)
                     /locus_tag="OQS10_001286"
     CDS             complement(15093..16253)
                     /locus_tag="OQS10_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2292363.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16491..17792
                     /locus_tag="OQS10_001287"
     CDS             16491..17792
                     /locus_tag="OQS10_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292364.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            17807..20170
                     /locus_tag="OQS10_001288"
     CDS             17807..20170
                     /locus_tag="OQS10_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCS2292365.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20350..21945)
                     /locus_tag="OQS10_001289"
     CDS             complement(20350..21945)
                     /locus_tag="OQS10_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS2292366.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22164..23084
                     /gene="ldtB"
                     /locus_tag="OQS10_001290"
     CDS             22164..23084
                     /gene="ldtB"
                     /locus_tag="OQS10_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS2292367.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23143..24261)
                     /locus_tag="OQS10_001291"
     CDS             complement(23143..24261)
                     /locus_tag="OQS10_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS2292368.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24258..24725)
                     /gene="mntR"
                     /locus_tag="OQS10_001292"
     CDS             complement(24258..24725)
                     /gene="mntR"
                     /locus_tag="OQS10_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS2292369.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            24911..25039
                     /gene="mntS"
                     /locus_tag="OQS10_001293"
     CDS             24911..25039
                     /gene="mntS"
                     /locus_tag="OQS10_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS2292370.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25311..26894
                     /gene="opgE"
                     /locus_tag="OQS10_001294"
     CDS             25311..26894
                     /gene="opgE"
                     /locus_tag="OQS10_001294"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS2292371.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(26943..27458)
                     /gene="ompX"
                     /locus_tag="OQS10_001295"
     CDS             complement(26943..27458)
                     /gene="ompX"
                     /locus_tag="OQS10_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS2292372.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27511..27576)
                     /locus_tag="OQS10_001296"
     CDS             complement(27511..27576)
                     /locus_tag="OQS10_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292373.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            27811..28698
                     /gene="rhtA"
                     /locus_tag="OQS10_001297"
     CDS             27811..28698
                     /gene="rhtA"
                     /locus_tag="OQS10_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS2292374.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            28997..29500
                     /gene="dps"
                     /locus_tag="OQS10_001298"
                     /gene_synonym="pexB"
     CDS             28997..29500
                     /gene="dps"
                     /locus_tag="OQS10_001298"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS2292375.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            29904..30650
                     /gene="glnH"
                     /locus_tag="OQS10_001299"
     CDS             29904..30650
                     /gene="glnH"
                     /locus_tag="OQS10_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS2292376.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            30789..31448
                     /gene="glnP"
                     /locus_tag="OQS10_001300"
     CDS             30789..31448
                     /gene="glnP"
                     /locus_tag="OQS10_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS2292377.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            31445..32167
                     /gene="glnQ"
                     /locus_tag="OQS10_001301"
     CDS             31445..32167
                     /gene="glnQ"
                     /locus_tag="OQS10_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS2292378.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32284..34509
                     /gene="ybiO"
                     /locus_tag="OQS10_001302"
     CDS             32284..34509
                     /gene="ybiO"
                     /locus_tag="OQS10_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS2292379.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(34506..35432)
                     /gene="rlmF"
                     /locus_tag="OQS10_001303"
     CDS             complement(34506..35432)
                     /gene="rlmF"
                     /locus_tag="OQS10_001303"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS2292380.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            35708..35968
                     /locus_tag="OQS10_001304"
     CDS             35708..35968
                     /locus_tag="OQS10_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2292381.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36233..>36523
                     /locus_tag="OQS10_001305"
     CDS             36233..>36523
                     /locus_tag="OQS10_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292382.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKN"
BASE COUNT         8806 a         9894 c         9208 g         8615 t
ORIGIN      
        1 gcaatattca aacgtaacta tcctttaatt ttcggatcca gcgcatcgcg taaaccatcg
       61 cccaacaaat tgaacgccag tacggtcaga aaaatagcca gggccggaaa aacagcgaca
      121 tgcggcgcga taaccatatc cgctcgtgcc tcattgagca ttgctcccca ctctggtgtc
      181 ggcggctgcg caccgaggcc gagaaatgac aggctggcgg cagagataat cgaggtgcca
      241 atgcgcatgg tgaaaaacac cacgatagac gagacggtcc caggcaagat atgacgcaat
      301 aaaatggtca tatcgctggc accaatactg cgtgctgact caataaaggt ttgctgtttc
      361 aacaccagcg tgttgccgcg caccagtcgg gcaaacgcgg ggatggaaaa aatggcgact
      421 gcgataatca cgttagcaat gccgcttccc aacaccgcaa caacagcgat cgccagtaaa
      481 atacccggga aggcaaacag cacatcgcaa atgcgcatga tcagccgatc ccaccagcct
      541 tcataatatc cagccaacaa gcccagcaac gtcccgatcg ccgcaccgat aaacacggca
      601 aacacgcccg ccgccagcga gatttgcgca ccaaccagga cacggctgaa aatgtcgcgc
      661 cccagtgaat cgacgccaaa ccagtgctgc aaagaaggtc cgttattcag attgtcataa
      721 tcaaaataat tttcggcgtc atagggagcg atccagcgtg caaaaatggc caccacaatc
      781 aataaaataa cgaataatgc ggcggtcatc gccatatgct gacggcgaaa tcgtcgccag
      841 aattcatgcc acggtgtacg cacctggtca ggtttgacca gtggcatggc gtttaacacc
      901 gcctgacgtc gccagttaaa tagtcgcatc cttacttgta cctgatagcc gggttaatgg
      961 cggcgtaaag cacatccacc actaagttga taagaataaa ttccagcgag aaaagcagaa
     1021 tttccgcctg aatcaccggg taatcgcgca tttctacgga gtcaacgagt aagcgtccaa
     1081 gtccgggcca gttgaaaact ttctccacca cgatagaacc accgagcaaa aagccaaact
     1141 gtaagcccat catggtcact accgggatca tcgcgttacg tagcccgtgt ttgaggacaa
     1201 cccaggtttc gctcacccct ttcgccctcg cggtacgcat ataatcttcg cttaaaacat
     1261 cgacaaacga cgcgcgggta aagcgcgcca tcacggctgc cactgccgcg ccgagcgtca
     1321 gggagggtaa aatgtagtgc tgccaactgt ctgctcccac ggtaggcagc cagcccagtt
     1381 caacggagaa tacctgaatt aaaagcatcc ccagcgcaaa tgccggaaac gagatccccg
     1441 acaccgcaat ggtcatactc aatcgatccg gccaacgatt acgccagacg gcggcgataa
     1501 ttcccgccgc cataccaaat atcaccgccc agaccatact ggttatggtc agccacagcg
     1561 ttggcataaa gcggctggca atctcatcgg caaccggacg acgcgacacc atcgacaggc
     1621 caaaatcccc ctgcacagca ttgctgatat agtgccagaa ctggtgatac agcggctgat
     1681 ccaaccccag ctgctgacgc accagttcta taacctgcgc atcagcttcg agcccggcaa
     1741 tcaatcgcgc cggatcgccg ggcagcatat gcacaaataa aaacaccagc accgagacga
     1801 taaacagcgt cggaatcaac cccagtaagc gtttgataac gtaattaagc attccactcc
     1861 ctgcgttgct tattgcaaat ccgcgtcttc aaagctgaag ccggtgtctg gcatgatcca
     1921 aaaaccggtc aggtttttac tgtgtgccga caccagtttt tctaccacca gcgggatcca
     1981 cggcgactct tgccagatga tatcctgcgc cgccttatac aagcgggtct tttccgccgg
     2041 atcattagtt ttcagtgcct gagccaggaa gtcgtccacc tgtttattgc tgtaaaatgc
     2101 ggtattaaac agcgtcggtg gccagttttg cgaggcgaac agtggcgata gcgcccagtc
     2161 cgcttcgccg gttgaagccg accagccagt gtagaacatc cgcacgccgc tctctttttg
     2221 ccctttacct tcaacttctg ccgcccgctg tccggcatcc atcgcggtca cctgggcttt
     2281 aatcccgacc tgcgctaact gctgctgggt aaattgcagc actttctgcg cggtgctgtg
     2341 gttatgtgac gaccacagcg tggtactgaa accgttggga tatcccgcct ctttcagtaa
     2401 ttcgcgcgct ttcactggat cgtaaggcca cggtttataa ctttgcgcgt aggcgatact
     2461 tggcggtacc acaccagtag ctggcgttgc atagcccgca aaggcaactt tcaccagcgc
     2521 cggacggtta atggcgtaat tcagcgcctc acggaccttc gggttatcga acggcttttg
     2581 cgtcacgttc atactgatat aacgctgcat aattgacgga ctggccatca actcgatatt
     2641 tttgtttttc tccagcagtg tggcctgctc gtagggaatg gggaaagcaa actgcgcttc
     2701 accggtttgc agcattgccg cgcgggtgtt gttatccgcc accggacgcc aggttatgct
     2761 gtccagtttg ggcaatcctg gctgccagta acccgcgaat tttttcacct tcacaaaatc
     2821 ggtctgattc caggtatcca gttcatacgg tccggttccc accggatgaa aaccaatctc
     2881 cttgccatat ttttccagcg ctgccggtga aatcatcgcg gtcgccggat gggcaagaat
     2941 attaataaac gctgagaacg gctgtttgag ggtaatcttt accgttgtcg gatcgatcgc
     3001 ctccgttttg gcaatattct tatacaggtt atagcgttta agatgattcg ccggatcgct
     3061 ggcccggtcc agattcgctt ttaccgccac tgcgttgaaa tcggtgccat cctggaattt
     3121 aatgccttcc cgcaatttca cggtgtaagt aatgccgtca tcggaaacgg tataactctc
     3181 cgccagcacg tttttcagtt tcatctcttt atccagaccg aacagcccct ggtaaaacga
     3241 tttcgctacg gcctgagata acgtatcatt cgcgtcatac ggatcgagcg tggtgaaatt
     3301 cgatcctacc gccaccacca catctttggc agcgaatgca caagatgcca tcaacgctgt
     3361 cgcaatgccc agcgccacta acccactacg gtgtacagct cttgccattt tattctccgc
     3421 ctgaatgtta tctacgcatg aatgcgtatt ctgattgtgg ttgtgcgacg taatgccccg
     3481 gcccgacgca ttgcaacgag acggctgcca cctcttcgcc acgcagatga atattgctgg
     3541 gaagatcgtc cgacagcagt acacgctgcg gtcgttgtcg ggacggttca gcgaccggaa
     3601 ctgccgccag taatttacgc gtataaggat gctgcgggtt ttcgaagacc gcgcgccgtg
     3661 gaccaatttc aacaatttgc ccgagataca tcaccgccac acgatgacta atccgctcta
     3721 ccacggccat atcgtgggag ataaacagat acgcaatgcc gaaatcacgc tggagatcga
     3781 gcaacaagtt gataatctgc ccgcgaatag aaacatccag cgccgaaacg gcttcgtcgg
     3841 caatgatcac ttttggattc aatgccaacg cgcgagcaat gcagatgcgc tggcgctgac
     3901 cgccggaaaa ctcatgcggg taacgccagg catgttcagg taacaggccc acgcgctcca
     3961 gcaaccacgc aacgcgtgca gccgcgtctt tacctggcaa taaaccgtgt acacgcagcg
     4021 gttcgataat cgaatcaccg atggtctgac gtgggtccag cgaagcgtaa gggtcctgaa
     4081 aaataaactg aatatcccgg cgcaatgcct gaagtttgcc gggtgacaag gtatcgattc
     4141 tctgaccgtt aaagataatt tcgccgccct gcgattcgac caggcgcagc aacgcccgcc
     4201 cggtagtgga tttaccgctg ccagactcgc ccaccagcga tagcgtttcg ccaggccaga
     4261 gatcaaaact gactttctca acggcatgca cttcccgcgt tacgcgattc aacaaaccgc
     4321 tgcgcaaagg gaaacgggtg acaagattac gcactcgtaa aacaggttcg ccatccacca
     4381 ccgttttctg ctcgatgggg ggggcctgtt tcgctggatg ttcaagcgat atcaacggga
     4441 aacgtcgggg ataatccaac cctttcatcg caccaagttg cggaacagca gctaacagcg
     4501 cacgggtgta aggatgttgc ggtgcatgaa aaatctgttc gacggtaccc gtttccaccg
     4561 cctcgccctg atacatcacc agtacccgat cggcaatctc tgccaccacg cccatatcgt
     4621 gagtgataaa gataacgccc atcgacatct ctttttgcaa tactttgatt aattgcagga
     4681 tctgcgcctg aatagtgaca tccagcgcgg tggttggctc atcggcaatc agcaccgccg
     4741 ggcggcatga cagcgccatc gcaatcatca ctcgctggcg catcccgcca gagagttgat
     4801 gcggataacg tgaaagaatg gtttgtgcct caggaatgcg tacctgatcc agcatccgct
     4861 tcgcctcgac catcgcttct tcacgactgg cgttctgatg cagacgaatt gattcggcaa
     4921 tctgttcacc cacagtaaat accgggttca gcgatgtcat cggctcctga aatatcatcg
     4981 ccatatccgc accgcgaaca tggcgcattt gtgcagcgct ctgctcgcta agttcaatca
     5041 cttcgcgact gcgccgctgc aacagcattt tatcgcactg tactaaaccg cccgcctgtt
     5101 ccaacaggcg catcaatgcc aacgcagtca ctgacttacc ggagccggat tcgccaacaa
     5161 ttgccagcgt ctcaccgcgt tgcagactaa aagagagatt gcggaccgca gctattttct
     5221 gctggtcctg cataaaggca atattcagat tttcaaccgc cagcacatta ccggcatcaa
     5281 gttcatcact gtgtggcaac ggtgtcccct ttttcacggt agataccggt ggttggcgta
     5341 tcgcctgcgt agccccaggc gcgatacatt ccttcggtgt taaacggtag cgcgacattc
     5401 ccttcatggt cgatagcgat taagccaccg ctaccgccaa gcgcagggag tttttccatt
     5461 actacccgct cgcaggcttc cgcgagactt aatccgccgt aatccattaa cgcggcgatg
     5521 tcatatgccg ccagcgcgcg gatgaagact tcgcccgtgc cgatacaaga aaccgccaca
     5581 ctggcgttat tggcgtagca tccggcaccc actaagggac tatcgccaac tcgtccgggt
     5641 aatttattgg tcattccgcc tgtggacgtg gctgccgcca aattgccgtc taaatccaac
     5701 gccacggccc ccacggtgcc cattttctgt ttttcatcca gtggcgcacc gctatggtcg
     5761 aggacggttg ccccttcctt gcgcgctgcc agtagttgtt cataacgcaa aggcgtggag
     5821 aaaatctccg gcgagacgcg ctccatgcca cgagcaaacg caaaattttc tgccccttcg
     5881 ccaatcatca tcacatgcgg gctttgctcc atcaccagcc gggcggcaag aaccggatta
     5941 cgcagatgac taacgcccgc caccgcaccg gctttcaggg tgttaccatc catcacacag
     6001 gcgtccagtt catgggtttc atcacgcgta aagacagcgc caattccggc gttaaacagt
     6061 ggacactctt ccagcagacg caccgcttcc gtcaccacat ccagcgcact ttcgcccgct
     6121 tccagcattt tctgcccggt ttcaacaatg gcagacaacg cctcaatgta gcgtaattcc
     6181 tgttgcagac tcatctgcgc gcggctaatt gcacctgcgc caccatgaat tgcaatgact
     6241 gctttgccca tagtattcgt ccattaaggg tggctcgcca cttttgctat aaatatcaga
     6301 atttttatga attactcatt caatttttga atatagaaag atggatatgg catgtaaagg
     6361 caggagccaa cgctacgccc gaggtggcat acagtctgtg gcgtttttct gacataatag
     6421 gcaaattcga ttttgcctcc gcaggagtgt tttcatggaa tttaccaccg gattgatgtc
     6481 gctcgacacc gcgcttaatg agatgctttc tcgcgtcacc ccactgaccg cccaggaaac
     6541 gctgccactg gtacagtgtt ttggtcgtat tctggcgagc gatgtcgttt cgccgcttga
     6601 tgttccgggg tttgataact ccgcaatgga cggctacgcg gtgcgtttag ccgatattgc
     6661 ctccgggcaa ccgctgcccg ttgccggtaa atcctttgcc ggtcagccgt atcatggtga
     6721 atggcctgcg ggcacctgca ttcgtattat gaccggtgcg ccggtgccgg aaggctgcga
     6781 agcggtggtg atgcaggagc agactgaaca aacggacaat ggcgtgcgtt ttactgctga
     6841 agtgcgtagc gggcaaaata ttcgccgtcg cggtgaagat atctctgcag gtgcggttgt
     6901 tttcccggcg ggaactcgcc tgactaccgc agagctgcca gtaattgctt cactggggat
     6961 tgccgaagtt ccggtgattc gtaaagtgcg tgtagcgctt ttttctaccg gtgatgaact
     7021 ccagttgccc ggtcagccgc tgggcgacgg ccaaatctac gacaccaacc gtctcgccgt
     7081 acacctgatg ttagaacagc tgggttgcga ggtaatcaac ttagggatta tccgcgacga
     7141 tccccatgcc ctgcgcgccg catttattga agccgacagc caggcggatg tagtgatcag
     7201 ttccggcggt gtttcagtgg gtgaagcgga ttacaccaaa acgattcttg aagagctggg
     7261 ggagatcgcc ttctggaagc tggcgattaa accaggtaaa ccgttcgcgt tcggtaaact
     7321 cagcaatagc tggttctgcg gcctgccggg caacccggtt tcagcgacgc tgaccttcta
     7381 tcaactggta cagcctttgc tggcaaaact aagcggcaac accgccagcg gcctgcccgc
     7441 gcgccagcgc gtacgcacag cgtcccgcct gaaaaaaacg ccaggacgtc ttgatttcca
     7501 gcgcggcgtg ctgcaacgca acgccgacgg tgaacttgaa gtgacgacca ccggacatca
     7561 gggttcacat atatttagct cctttagtct cggcaactgc tttatcgtgc tggaacgcga
     7621 tcgcggcaat gtggacgtgg gcgaatgggt ggaagtagaa ccgtttaacg cgttgttcgg
     7681 aggcctgtaa tggcggaact cagcgatcag gagatgctgc gctacaaccg gcaaatcatt
     7741 ctgcgcggtt ttgattttga cggtcaggag gcgctaaaag attctcgtgt gctgatcgtc
     7801 ggcctcggtg gactcggctg cgcagcttcg ctgtatctgg caagcgccgg tgtcggcaac
     7861 ctgacgctgc tcgacttcga cacggtttcg ctctcgaatc tgcaacgcca gacactgcac
     7921 agtgatgcca cggtcgggca accgaaggtg gaatccgccc gtgacgccct gacgcggatc
     7981 aacccacata tcgcgattac gccagtcaat gcactgctag atgacgcaga acttgcagca
     8041 ttgattgctg aacacgatct ggtgctcgac tgtacggata acgttgcggt acgtaatcaa
     8101 ctgaacgcag gctgttttgc cgcgaaggtg ccgctggttt ccggcgcggc aattcgtatg
     8161 gaagggcaaa tcacggtctt cacttatcag gacggcgaac cgtgctatcg ctgccttagc
     8221 cgtttgtttg gcgagaacgc attaacctgc gtagaagcag gcgtgatggc accgttgatc
     8281 ggcgtaattg gttcgttgca ggcaatggaa gcgatcaaac tgctggcagg ttatggcaaa
     8341 ccgaccagcg ggaaaatcgt catgtacgat gcgatgacct gtcagtttcg cgaaatgaaa
     8401 ctgatgcgca atccggggtg tgaggtgtgc gggcagtaat tgccacctcc aggcctgata
     8461 agacgcgaca agcgtcgcat caggcatttg ctccagacgc cagatgcggc gtgaacgcct
     8521 tatctggcct acggttcggg cacagatttc caagcgcggc aggcatcggt gcactccgcc
     8581 acgtcatcag caatttatga ggatgcagaa cgtccggtta aatcgacgtt ctgccaaacg
     8641 ctccctgcca gtcctgctca aacttcgcca cagcggcttc caccgccgga tagctaatca
     8701 tctgttgtgc cacatccagt ggcagagtaa ttgattcaca tcctgccagt aagcagtcca
     8761 gcgcctgacg cggggttttg aaactcgctg ccagcacttt cgcctgcggc gcatgcattt
     8821 tcaataactg gtgtaagtcg gtcacagtct gaatgccgct accgccctga gcatcaatac
     8881 gattaacgta aggcgcaaca tattccgcac ctgccagcgc cgacagcaac ccttgtgctg
     8941 cgccatatac cgcggttccc agcgttggaa tcccttccgc ttttaacatc ttaatagctg
     9001 ccagcccctc ggcggtcacc ggaactttca ccacgatatc cgcaataata gaacgcaatt
     9061 tacgtgcgtc attcaccatc ccttcagcag tggtagccat tacctgggca aacagacgcc
     9121 cctgaccgcc catcgcttca tgaagttgcg gaagcacaac atccagcggt tttttacccg
     9181 cggcgataat gcttgggtta gtggtcacac ccgccagcgg aaaaatacgt gacagcgcct
     9241 tcaccgcaac aacgtctgaa gtatccagat acagttccat aaccatcctc aaaatgtact
     9301 ttttgtctga atgtagacta tcacgggaaa actctctgaa cagttgacgc acatcaatct
     9361 aactttcatt cgaaagtatt ttaatgtttg aacgaaacag agaggcgact atgattttca
     9421 atattcagcg ctactcgacc catgatggcc ccggtatccg cacggtcgta tttcttaaag
     9481 gctgttcgct gggctgccgc tggtgtcaga acccggaaag ccgcgcccgc acgcaggatc
     9541 tgctgtatga cgcacgactg tgtctggaag gctgcgagct gtgcgctaag gccgcgccgg
     9601 aagtgattga gcgcgcgctg aatggtttgc ttattcatcg ggaaaagtta accccggagc
     9661 atctgacggc gttaaccgac tgctgtccga cacaggcatt aaccgtgtgt ggtgaagtga
     9721 aaagcgttga ggagatcatg acgaccgttc tgcgcgataa accgttttac gatcgcagcg
     9781 gcggcggttt aacgctttcg ggtggtgagc cctttatgca gccggaaatg gcgatggcgc
     9841 tactgcaagc cagccacgag gcaggcattc atactgcggt agaaacctgt ctgcatgtgc
     9901 cgtggaaata tatcgcccct tctctgccct atatcgatct gtttcttgcc gatttaaaac
     9961 acgttgccga cgcgccgttt aaacagtgga ccgacggtaa cgccgccaga gtgctggata
    10021 acctgaaaaa actcgccgca gcgggcaaaa aaatcattat ccgcgtgccg ctgattcagg
    10081 gctttaatgc cgacgaaacc tctgtaaaag ctattaccga ttttgccgcc gacgagctgc
    10141 acgttggcga aattcatttc ctgccctacc acacgctagg catcaacaaa tatcacttac
    10201 ttaatctgcc ctatgacgcc ccggaaaaac cgcttgatgc gccagaactg ctcgactttg
    10261 cccagcagta tgcctgccag aaagggttaa ccgcgacctt acgaggataa caaccatgac
    10321 cacactgaaa ctggacacgc tcagcgaccg cattaaagcg cacaaaaatg cgctggtgca
    10381 tattgtgaaa ccgccagtct gtaccgagcg cgcgcagcac tataccgaga tgtatcaaca
    10441 acatctcgat aagccgatcc cggtacgtcg cgcgctggca ctggcgcatc acctggcgaa
    10501 tcgcaccatc tggatcaaac acgatgagtt gatcattggc aaccaggcaa gcgaagttcg
    10561 cgccgcgccg atcttcccgg aatatactgt ctcgtggatc gaaaaagaga ttgatgatct
    10621 ggcagatcgt cccggtgctg gctttgcggt gagcgaagag aacaaacgcg ttctgcatga
    10681 agtgtgcccg tggtggcgcg gtcagaccgt acaggatcgc tgctacggca tgtttaccga
    10741 tgagcaaaaa ggtctgctgg cgaccggaat cattaaagcg gaaggcaata tgacctccgg
    10801 cgatgcgcac ctggcggtga atttcccgct gctgctggaa aaaggtcttg atggtctgcg
    10861 cgagaaagta gcggaacgtc gctcgcgcat caatctgacg gtactggaag atttgcacgg
    10921 cgaacagttc ctgaaagcga ttgatatcgt gctggtggca gtcagcgaac acattgaacg
    10981 ttttgcagcc ctggcgcgtg aaatggccgc gaccgaaacc cgcgaaagcc gtcgcgatga
    11041 actgctggcg atagcagaaa actgcgatct tatcgcccac cagccgccgc agactttctg
    11101 gcaggcgctg caactgtgtt acttcatcca attgatattg cagatcgaat ctaacggtca
    11161 ctcggtatcg tttggtcgta tggaccagta tctctacccg tactatcgcc gcgacgttga
    11221 actcaaccag acactggatc gcgaacacgc catcgaattg ctgcatagct gctggctgaa
    11281 actgctggaa gtgaacaaga tccgctccgg ctcacactca aaagcctctg cgggaagtcc
    11341 tctgtatcag aacgtcacca ttggcggaca aactctggtt gatggtcaac caatggacgc
    11401 ggtgaatcca ctctcttacg ctatcctcga atcctgcggt cgcctgcgtt ccactcagcc
    11461 taacctcagc gtgcgttacc atgcaggaat gagtaatgat ttcctcgacg cctgcgtaca
    11521 ggtgatccgt tgcggcttcg ggatgccggc gttcaacaac gacgaaatcg tgatcccgga
    11581 atttattaaa ctcggtattg aaccgcagga cgcttacgac tacgcagcga ttggttgtat
    11641 cgaaaccgcc gtcggtggca aatggggcta tcgctgtacc ggcatgagct ttatcaactt
    11701 cgcccgcgtg atgctggcgg cgctggaagg cgggcgtgat gccaccagcg gcaaagtgtt
    11761 cctgccacaa gaaaaagcgt tgtcggcagg caactttaac aatttcgatg aagtaatgga
    11821 cgcgtgggat acgcaaatcc gttattacac gcgtaaatca atcgaaatcg aatatgtcgt
    11881 cgacaccatg ctggaagaga acgtgcacga tattctctgc tcggcgctgg tggatgactg
    11941 tattgagcga gcgaaaagta tcaagcaagg cggcgcgaaa tatgactggg tttctggcct
    12001 gcaggttggc attgccaacc tcggcaacag cctggcggca gtgaagaaac tggtatttga
    12061 acagggcgcg attggtcagc aacagctggc tgccgcactg gcggatgact tcgatggcct
    12121 gactcatgag caactgcgtc agcggctgat taacggtgcg ccgaagtacg gcaacgacga
    12181 tgataccgtc gatacgctgc tggctcgcgc ttatcagacc tatatcgacg aactgaaaca
    12241 gtaccataac ccgcgctacg gtcgtggtcc ggttggcggc aactattacg cgggtacctc
    12301 gtctatctcc gctaacgtac cgtttggcgc gcaaactatg gcaacaccgg acgggcgtaa
    12361 agcccacacc ccgctggcag aaggcgcaag cccggcctcc ggtactgacc atcttggccc
    12421 tactgcggtc attggctcag tgggtaaact gcctacggca gcgattctcg gcggcgtgtt
    12481 gctcaaccag aaactgaatc cggcaacgct ggagaacgaa tctgacaagc agaaactgat
    12541 gatcctgctg cgtaccttct ttgaagtgca taaaggctgg catattcagt acaacatcgt
    12601 ttcccgcgaa acgctgctgg aggcgaaaaa acatccggat cagtaccgcg atctggtagt
    12661 gcgtgtcgcg ggctattccg ccttcttcac cgcgctctct ccagacgcac aggacgatat
    12721 catcgcccgt actgaacata tgctgtaatc tcctcggccc ggcggcgtcg tcgccgggct
    12781 aaatcattca catcatcaat ttcatcctta ctttcattcg aaatataatt tgtgctctgc
    12841 gtcacattgt tattacactg ttacggtttc attttcgagc caggagcaca cctatgagcg
    12901 taaaagttat cgtcacagac atggacggta cttttcttaa cgacgccaaa acgtacaacc
    12961 aaccacgttt tatggcgcaa tatcaggaac tgaaaaagcg cggcattaag ttcgttgtcg
    13021 ccagcggtaa tcagtattac cagcttattt cgttctttcc tgagctaaag gatgagatct
    13081 cttttgtcgc ggaaaacggc gcactggttt acgaacatgg caagcaactg ttccacggcg
    13141 aactgacccg acatgaatcg cggattgtta ttggcgagtt gctaaaagat aagcaactca
    13201 attttgtcgc ctgcggtctg caaagtgcat atgtcagcga aaatgccccc gaagcatttg
    13261 tcgcactgat ggcaaaacac taccatcgcc tgaaagctgt aaaagattat caggagattg
    13321 acgacgtact gttcaagttt tcgctcaacc tgccagatga acaaatcccg ttagtgatcg
    13381 acaaactgca catagcgctc gatggcatta tgaaacccgt caccagtggt tttggcttta
    13441 tcgacctgat tattcccggt ctacataaag caaacggtat ttcgcggtta ctgaaacgct
    13501 gggatctgtc accgcaaaat gtggtagcga ttggcgacag cggtaacgat gcggagatgc
    13561 tgaaaatggc gcgttattcc tttgcgatgg gcaatgctgc ggaaaacatt aaacaaatcg
    13621 cccgttacgc taccgatgat aataatcatg aaggcgcgct gaatgtgatt caggcggtgc
    13681 tggataacac atcccctttt aacagctgac ctctctaccg gagccgtgcg ctccggtttt
    13741 cccttctttt tacatcctgt acatcacatc aaatcattaa acttgcatta acttatttat
    13801 aacttatatt gtcgcatgca aatcagaatg gtttcgaatg aaagttaatg agaggtggtt
    13861 atggcttcta cttttaccag cgacacattg cctgccgatc acaaagcagc tatccgtcag
    13921 atgaagcacg cgctgcgggc gcagcttggc gacgtccagc agatctttaa tcagctaagc
    13981 gatgacattg ccacgcgagt ggctgaaatc aacgcactca aagcacaggg cgatgccgtc
    14041 tggccggtgc tgtcttatgc cgatatcaaa gcaggtcatg ttactgcaga gcagcgcgaa
    14101 cagattaaac gtcgcggttg tgcggtgata aaaggccatt tcccccgcga acaagcgcta
    14161 ggctgggatc agtcgatgct ggactatctg gaccgcaacc gctttgacga ggtctacaaa
    14221 ggccccggcg ataatttctt cgggacgctc agcgcttcac gtcccgagat ttaccccatc
    14281 tactggtcgc aggcgcaaat gcaggcccgc cagagtgaag aaatggcgaa tgcgcagtcg
    14341 tttctcaatc gtctgtggac atttgaaagt gatggaaagc aatggtttaa cccggatgtg
    14401 agcgtcatct accctgaccg tatccgccgc cgtccgcccg gaacgacctc caaaggtctt
    14461 ggagcgcata ccgactccgg ggcgctggaa cgctggctgc ttccagcgta tcagcgcgtt
    14521 ttcgccaacg tctttaatgg caatctggcg caatatgatc cctggcatgc ggcacatcgt
    14581 acggaagttg aagagtacac ggtggacaac accaccaaat gttccgtgtt tcggacattc
    14641 caaggctgga cagcgctctc taatatgctg cctggtcagg ggctgctgca cgttgtgccc
    14701 attcctgaag ccatggcgta cgtactgtta cgtccgctgc ttgatgatgt gccggaggat
    14761 gaactgtgcg gcgtagcgcc cggaagagtg ttgccggtat cagagcaatg gcatccactg
    14821 ttaattgagg cgttaaccag cattccaaaa ctcgaggccg gagactccgt ctggtggcac
    14881 tgcgacgtca tccattccgt tgcccccgtt gaaaatcaac agggttgggg caacgtgatg
    14941 tacattcctg cggcaccaat gtgcgagaaa aatcttgcct acgcgcacaa ggtgaaggcc
    15001 gcactggaaa aaggcgcatc gccgggcgac ttcccgcgcg aggactatga aacaaactgg
    15061 gaaggacgct ttacgcttgc cgacctcaac attcacggta agcgagcgtt gggaatcgac
    15121 agctgagtgt cgtacagccc ttcccgttcc acggcggtgt tcccttttcc tgggcgaacc
    15181 cgccgcactg actcccgcac cgccaatcgt ccgtgcagca gcagcgagcc caccggagca
    15241 tccgggcgaa tcagccgctg ttgcaacatt tgcaccgctt cattgcctag ctcatcgcgt
    15301 ggcacatgca cagccgtcag gggaacatcc tcgattgccg caaggttaaa gccgtcgata
    15361 ctcatcaccg agacatcctg tggtacgcgc agaccacgac gatgtagcgc atttaccgtt
    15421 cccgccgcca taaaatcccc gccgaccaga aacgccgtcg gcaacgtctt gccattggcg
    15481 gcatccagcc acgctccgac cttttcttcc gcttctttag cgctaaaact gctgatattc
    15541 agcaaatgac ggtcatggtg aaaggccaga ttatgctgac gccatgattc tttaatccct
    15601 gcaaaccgct gctccatagt gtagcggcgc aggcacatca cattcatcac ctcccggtga
    15661 cccatttcaa acagatagcg ggcggcgaac gcgccaatta accgatgatc cggcgcaatc
    15721 gccgggaggc gcattcgttc atcacggcag ttgatcagca cacaaggctt agcgagatcg
    15781 gcggccagat catggatgtg cggatcgtca atccccagca ggatcgctgc ctgcgtatct
    15841 gcttcattca tccgcgagag aaagagcgcg ggatcgctgt caaactcctc cagcgcgcag
    15901 taacgcagcc gcacttcatg cggcgataac gctttgctga tgctctggat cacacggtaa
    15961 taaaagatat cagatcgttg gtcaaaagcg cgttgcggcg cgaaaataat caggttgttc
    16021 agcaacatac gacctgctgc catgccatcc atcacgccca actcacgcgc gcaggccagc
    16081 acctgttcgc gagcgcgtgc actggtattc gctttgccag ccagcacccg cgaaaccgta
    16141 ctcggcgata gcccggtacg tgcggcgata tcggcgattt tcagcttctt gtccattttg
    16201 tgatctgcct ctaattcgga aatgacagaa ttttcatgag caaaaaagcc cattctgtct
    16261 ggcattaaat ccatttcagt aatgtatatc agtcactcat ggaaaaacgt gcacaaaacc
    16321 cgctttcttt cgcttttagg gtctcttact gtgaattggt agaacaactt ccatacaaaa
    16381 ataatggaaa aacgaacaat aaagacttta tttatcaaaa agttaaaagt ggtgtaacaa
    16441 ctccattctg acgttgccgc acaaacccta taccattctg tggagtgcat atgagtcagg
    16501 gtatcaataa cgatatgact gccagtaaac cgcgccatat cgtcaaaaat ctgcgttggt
    16561 ggatactggt cctttttttg ctcggtgtta ctgtcaacta catcacgcgt aactcgctgg
    16621 gtattctcgc ccccgaactg aaaagcagcc tagggattac caccgaacaa tattcctgga
    16681 ttgtaggtgc tttccagctc gcctataccc tattccaacc gttgtgcgga tggctgattg
    16741 acgtcattgg cctcaaactg ggctttatga tctgcgcggg actgtgggca ctggcctgtc
    16801 tgatgcatgc gggggcggga aactggggac atctggcgct gttgcgcttc tttatgggcg
    16861 gtgcggaagc cgcggcaacc ccagcaaacg ccaaaaccat cggtgaatgg tttccaaaat
    16921 cagaacgccc gatcgccgca ggctgggcag gcgtcggttt ttccatcggt gccatgctgg
    16981 ccccaccgat tatctatttt gcccacgctt cattcggctg gcagggtgca tttatgttca
    17041 ctggcatcct ggcgatgctg tgggtggtgc tgtggtgggc gttctatcac agcccggata
    17101 agcatcctaa cctcagcaaa gccgagctgg actttattcg ccaggataac gaagcgcccc
    17161 cggtcaagtt accctttctg accgcactga aaaccgtggg caaaaataaa cgcttttacg
    17221 gtatcgctat cccggccttt atggcagaac cggcgtgggc ggtgatgagt ttctgggtgc
    17281 ctctatacct gtccaaaacc tatggcatgg acctgaagca gatcgcatta ttcgcctggt
    17341 tgccgttcct cgccgctgat cttggcagta ttgccagcgg ctatctgacc aaactttaca
    17401 cccgcctgtt tgggctgagt cgtgtgaact ccgttgtcgc atcatcggtc agtggcgcat
    17461 tcttgatggt ttcgctggcg gtgatggcct ttaccaataa cccgtatatc gccatcgtcc
    17521 ttatctccat cggcggcttc ggtcaccaga tcatctcctg catgctgagc gcactggtgg
    17581 tggaatcctt cgacaaaggt cagatggcca ctgttaacgg catgcgcggt tctgccgcgt
    17641 ggatcgcaag tttcctgttc tccctgatta tcggggttac cgctgacaaa atcggcttta
    17701 accccctgtt tatcgccatg ggcttctttg acctgattgg cgctttcttc ctggtcacct
    17761 ttattgctga acgtcgcgca aaacgcgcct gagagtggat gtaaacatga aaacgctgaa
    17821 aaactggacc ctgcacaaac agtctgccca ccacgttgaa ctgctggtcg atggccagca
    17881 tatcctgtgc ctgtacgtgc tggaagaaaa tctgttccgc gtgctgttaa aacgcaaagg
    17941 cgagctggcg ctagatcgca cctggagcat tgctccggcg caggatgtgc catgggaagg
    18001 gcgcagccgc gaagatctca gcggctttag cctgcccgcg tggacagtga tccagcacga
    18061 cgacaccgtg gttatcgcca ccgagtcact gcgtgcaacc gttcaccagc cgctgtggct
    18121 ggagtggcac tatcgtaaca acgaaggcga atggctgccg ttggtgaatg accgcccgac
    18181 cagcgcttat ctgctgaatg cccacggcga tggcgtggca cactatcaga gccgtcgtaa
    18241 agatgaacgc ttttatggcc tcggagataa agccggcgat ctgcaacgca acggtaaacg
    18301 ttacgagatg cgtaacctcg acgcgatggg ctacaacgcc gttagtactg acccgctgta
    18361 caaacacatt ccattcacca tcacccgccg cgatgacatt agcttcggcc tgttttatga
    18421 caacctcagc agctgctggc tggatctggg caacgagatc gacaactacc acaccgccta
    18481 tcgccgctgg caggcggaag cgggtgatat cgactactac ctgtttaccg gcgaacgcgt
    18541 acttgatatc accaaagcct tcgtacgcct gaccggaaaa accctgttcg gaccgaaatg
    18601 gagcctgggc tacagcggtt caactatgca ctacaccgat gcgccggacg cacaaaatca
    18661 gttgatgaac tttatccgcc tgtgtgaaga acatgcaatt ccctgcgatt cgttccagct
    18721 ctcttccggt tatacctcaa ttaacggcaa acgctatgtg ttcaactgga actacgacaa
    18781 agtgccgcag ccgaaagtga tgagccaggc atttcacaat gctggtttgc acctggccgc
    18841 caatatcaag ccgtgcctgt tgcaggatca cccacgctat aacgaagtgg cagaaagagg
    18901 actgttcatc cgtgattcag aaacagatga accagaacgt tccagcttct gggatgacga
    18961 aggttctaac ctcgagttta ctaatccgca gaccatccag tggtggcagg aaggcgtgac
    19021 cacacagctt ctggagatgg gtatcgactc cacctggaac gacaacaacg aattcgaagt
    19081 atgggacggt gaagcacgct gtcacggctt tggtcagacg atcgcgatca aacatattcg
    19141 cccagtgatg ccactgctga tgatgcgagc ctcgatggaa gcccagcagc gtttcgctcc
    19201 ggaaaaacgt ccgtacttga tctcccgctc cggctgcgcg ggaatgcagc gctacgtgca
    19261 gacctggagt ggcgataacc gcactagctg ggacactctt cgctacaaca cccgcatggg
    19321 tctggggatg agcctctccg ggctgtataa cctcggtcac gacgtcggcg gtttctccgg
    19381 tgataagccg gacccggaac tgtttgtacg ctgggtacaa aacggcgtga tgcatccgcg
    19441 ctttacgatt cactcatgga acgatgacca taccgtaaac gaaccgtgga tgtatccggg
    19501 cgtcactccg gcgatccgca gcgctattga gttgcgctat cgcctgctgc cctactttta
    19561 taccctgatg tggcaggcat acgccgatga tgaaccaatg ctacgtccga ctttccttga
    19621 ccacgagcac gacgttcaga cttttgaaga gtgtgatgac ttcctgcttg gtcgcgatat
    19681 tttggttgcc agcgtcgtag agcaaggcga gcgccagcgc cgtgtatggc tgccagataa
    19741 cgaaactggc tggtacgatt tctacaacgg cgaatggttc agtggcggtc agtggatcac
    19801 cctcgacgca ccgctggaga aactgccgct gatggttcgt gccggggctg gtctaccgct
    19861 cagcaaacgc atcacctatg tgagcgcaga acaagacgat acccgcgaac tgaaactgtt
    19921 cccgctgaaa ggtgtgggca caacgtccgg cctgctgttt gaagacgatg gcgaaagctg
    19981 gggctaccaa actggtaatg cgctttgggt ggaatgggaa atggtttgcg atggggcgac
    20041 catcaacctg aaagtcaacg cgcgcgggga ttatcgtcct gcatggagtg cactgaaagt
    20101 gtcattacca ttggaagaaa aacgcacgct gctggtcaat ggtgttgaag ggagtgaatg
    20161 gatgcgttaa atcattaccg gggccgggag gcgactgcac cttacccggt ccagccatcg
    20221 aatcctttac ctcagtattt cacgctgcag gaaggcagca aacctgtaaa ccccgggagc
    20281 ttaaataagt aagtgaccgg ggtgaacagg tgaagccaat gcatctgccg catgaaagac
    20341 ggcggggaat taagcgtcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa
    20401 gtcgatcgcg cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc
    20461 acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga
    20521 ctcgatggat tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt
    20581 ctgcatcatt aacttaccaa acagcatccg ccctttttcc ccaccggaaa gcactttagc
    20641 tggctttttg atgtcgtcct ggctgaacag caaacgaccg agaatgctgc gtaccgcctg
    20701 ctcgtcatcg ccttcctgct tccactgact catccattcg aacacggtca gatcattttc
    20761 aaactcatat tcgtggtcct gggcgtagta accaatgcgc gcgttttcag accattttac
    20821 ggtgccgctg tccggttgca gatcgcccac cagcgttttc agcagcgttg atttaccgac
    20881 gccgttggta cccagtaccg caagtttttc accgacctcc agcagcaggt tgagattttt
    20941 aaacagtgga ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa
    21001 cagtttctta tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc
    21061 ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc
    21121 gttggcgcta aagcggctaa cgaaagattg caactcagca atctgcgctt tcttcttggc
    21181 gttatcggcc agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt
    21241 acccggataa acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt
    21301 aaggaagtga cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg
    21361 ttccagccag cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat
    21421 ctccggatca gcaaacagcg cctgcgccag aagcacacgc agtttccagc caggagcaac
    21481 ttcactcatc gggccgtagt gctgttccac tggaattccc acgccaagca acagttcacc
    21541 ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat ttaacttcca gatcggccac
    21601 tttatagccg tcttcctcac tcatttccgg caaagcatag atgcggtcac gctcctgctt
    21661 cacttcccac aactctttat gccccatgat caccgtatcc agcacagtga actcttcaaa
    21721 ggcaaactga tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc
    21781 caaagtcggc tctaagtcgc cgccgaggat cttcataaag gtggatttac cactaccgtt
    21841 cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg acggaaatgt tttcaaacaa
    21901 cggcttactg ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa
    21961 agagatatga caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt
    22021 aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt
    22081 gagctggaat gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg
    22141 ccaggcacaa aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct
    22201 tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg
    22261 ggagtcgcct ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc
    22321 tggagtattt tgccgcggag taccagatgg ggctttccaa tatgatggaa gcgaacccgg
    22381 gtgtggatac cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc
    22441 tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact
    22501 atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag
    22561 atacgcctat caactggacc accaaagttg agcgtaagaa agcaggcccg acctggacgc
    22621 cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga accgcttccg gctgtcgttc
    22681 cggcaggtcc ggataacccg atggggctgt atgcactcta catcggtcgc ctgtacgcta
    22741 tccatggcac caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc
    22801 tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt
    22861 ttattgatga gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc
    22921 ataacccgct gtctaccacc gaagcccagt ttgaaggtca ggaaattgtg ccaattaccc
    22981 tgacgaagag cgtgcaaaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg
    23041 aagcgattaa aaaccgctcc gggatgccgg ttcgtctgaa ttaatcttca aaacttaaag
    23101 caaaaggcgg actcataatc cgcctttttt atttgccaga ccctagttgg ccgggagtat
    23161 aactaacaaa acatatccca ccaacgccgc ccacaacagc atcggtattg aatagagatg
    23221 gaaacgccac cagatgcggc gatcgttcgc catacgtagc gcaatcaaat ttgccagcga
    23281 tccgggtaat aacccaaagc cacctacgtt taccgcccat accagtaata aagacggcgg
    23341 cacatagttc agcaacaata tggtactcgg cacgttactg atcacctgcg ataaaccgat
    23401 tgccgttaac cataacccgg gttcagatag atgactcacg ttacccaaca cgccttgcaa
    23461 cgctggaagc tgggtcagta aatggacgtc gataaacatc gccataaaca ccagcagcag
    23521 cgtccagtcc acactgagca ccacgcgacg tgccagcagc gcaaagcctg ccgccacaat
    23581 caccagtccc cacagctctt gtttgaactc cagcgccgtc agaaagacga tatacagccc
    23641 cagacaactc cacaccagcc gcggtttcca ctccggtgtt tgcacccccg tatggtattg
    23701 cagcgccttt ccagggaaac aacaccagca caggagcagg agcgtcagca tcattgcgcc
    23761 agccagcggt gccatttggg caataaatcc ggcaaacgaa agaccagaac gtccccagat
    23821 aagaatattt tgcgggttgc caattggcgt cagtagcgaa ccagcgttga ctgccagcgc
    23881 ctcaaaaata atcagccgat taaccgggat ctcacacagt ctttttagcg tgatagtcag
    23941 cggaacaaca ataaacagcg cgacatcgtt ggtcagaaag gtagaaagca gcgccgccgc
    24001 cagcaccata aacatcgcca gccgacgctc cgtagcaaag cggcgcacca ttttgcgccc
    24061 cagcacatca aaataaccgc ttaactccac acctttggtc agcagcatca ggccgcttaa
    24121 ggtgatgatg gtgtgccagt cgatagcagc aggccaggat ttcggtgcaa agggcacgaa
    24181 aaagcttaat ccgataccaa caagaattaa taactgaaaa aaacgatcgc cttgcagcgt
    24241 gcgtaaaaaa ggcaggctca tttggcaccg tgtttctggg taaacaaacg aaaagcgtcg
    24301 agcgtctctt cactaacatg gtgctccatg ccttccgcgt cgcgacgggc gatttccgga
    24361 ctgacgccca acaccaacaa gaaattttcg actatctgat gtcgctcgcg gctttcctgc
    24421 gccagcttct ctccttctgc cgttaaaaac acgccacgcc aggggatcat ttcaatcagc
    24481 cccatggttg ccagccgctt aagcatttta gccaccgtcg gttgcgaaac tcccagacga
    24541 gcagccatgt ccacctgacg agcttccccc acttccctga tcaagtcaga aatcagctca
    24601 acgtaatcat caataagctc gcgccgatgc gcctctctga cctggcggaa cccttcaacg
    24661 tgctcttcca cgttcactaa ctgcgtcact ttttttgctg ttggcgtacc tgcgcgacga
    24721 ctcattgtgc ttcctcatta tggtgacgca tacatagcgt tctcatttcg tgcgcacatt
    24781 gtaaaccaga gttgcgaagg tacaaaaaat taacgtttta gcaatagcta cataatatag
    24841 cctgtgctat atctgtatgt aatgcaatca tccctcaagg atcgacggga ttagcaagtc
    24901 aggaggtctt atgaatgagt tcaagaagtg tatgcgcgtg tttagtcatt ctccctttaa
    24961 agtacggtta atgctgctct ctatgttgtg cgatatggtc aacaacaaac cgcagcaaga
    25021 taaaccttcc gataaatagc ggcgtcgcgg tacgccgctt cactcctgct tttatgcagg
    25081 cataacgcgt tttggtctga aaaaccccac tttttgtcgg atttgcaatc cccttcgcaa
    25141 aagatttgtt cgtcagtagt tgacctgaac ggcggctcgc tctatcttct tgcagcccta
    25201 cgtatattgc ggctcgcgga tgcggacccc tttcccctct tcacgcactc ttgcaggtat
    25261 tgacccttga cgccagggta agcacatggc gtttgttacg atagtggcat atgaatttaa
    25321 ccctcaaaga atcgcttgtt acccgtagcc gggtatttag cccgtggact gcgttctact
    25381 ttttacagtc gctattaatt aacctcggct taggttaccc cttcagtttg ctctacaccg
    25441 ctgcgtttac ggctattttg cttttgctat ggcgaacatt gcctcgcgta caaaaagttc
    25501 tggtcggtgt cagttcgctg gtggcggctt gttatttccc ttttgctcag gcctacggcg
    25561 cgcctaattt caatacattg ctggcattgc actccaccaa tatggaagag tcgaccgaaa
    25621 tcctgacgat ttttccgtgg tacagctacc tggtcggctt atttattttt gcgctcggcg
    25681 taatagcaat caggcgaaaa aaagagaatg aaaaagcgcg ctggaatacc ttcgacagcc
    25741 tgtgtctggt attcagtgtg gcgacatttt ttgttgctcc cgtgcaaaac ctggcctggg
    25801 gtggcgtatt taaactgaaa gatactggct atccggtatt tcgttttgct aaggatgtca
    25861 tcgtcaataa taacgaggtg attgaagagc aagaacggat ggcaaaactt tccggaatga
    25921 aagatacctg gacggtcact gccgttaagc cgaagtatca gacctatgtg gtggtgatcg
    25981 gcgaaagcgc gcgtcgcgat gccctcggtg cctttggcgg tcactgggac aataccccgt
    26041 ttgccagcag cgttaacggt ttgatatttg ctgactacat tgccgccagt ggctccacgc
    26101 agaaatcact tggcttaacg ctcaatcgcg tagtcgatgg caaaccacag tttcaggata
    26161 actttgtcac cctggcaaat cgcgcgggct tccagacctg gtggttttcc aaccagggcc
    26221 aaatcggcga atacgatact gctatcgcca gcatcgccaa acgtgcagat gaagtgtact
    26281 tcctgaaaga aggtaatttc gaagcagata aaaacacgaa agacgaagcg ttactggata
    26341 tgaccgctca agtgctggcg caagagcact cgcaaccgca gctgattgtt ctgcatctga
    26401 tgggctcgca tccgcaggcc tgcgacagga cacaaggcaa atacgaaacc tttgtgcaat
    26461 cgaaagaaac gtcgtgctat ctctatacca tgacgcaaac ggacgattta ctgcgcaagc
    26521 tgtacgatca gttacgcaac agcggcagca gcttctctct ggtttacttt tctgaccacg
    26581 gtctggcctt taaagagcgc ggcaaagacg tgcaatacct tgcccatgat gataaatatc
    26641 agcaaaattt ccaggtgcct tttatggtca tttccagcga cgataaagcg catcgggtga
    26701 ttaaagcccg ccgctcagcc aatgacttct taggcttttt ctcgcagtgg acgggaatta
    26761 aagcgaagga aataaatatc aaatacccgt ttatatctga gaagaaagcc gggccgatat
    26821 acatcaccaa cttccagtta cagaaggtgg attacaacca tctcggaacc gatattttcg
    26881 acccgaaacc ttaaaacaaa aaatccgccc cgagaggcgg attttttata tcaccaaagt
    26941 gattagaagc ggtaaccaac accggcaatc caggtgccta cgtcaacgct acgaatacgg
    27001 ctctgctcgt aagagaagtc cagagcaacg ttttccatcg ggttgaactg cagaccagca
    27061 ccgtaggaga aaccgtagtc gctggtgtcg tgtttgtagg tcgggtattc agtggtctgg
    27121 aatttaccat aacccacccc cactacaccg tagatgcttg cccagtcgtt aatgcggtaa
    27181 gccggaccag cagtgatgcc gtagtactgg tttttgttgt agtcaccaga gcttgcagta
    27241 cggcttttct cggtgtaagt gaaagaaccg atcacaccca gcgggctgtt gtcttcttca
    27301 tagcggtatt tcaggttgaa accgcccatt ttgttcattt ggccctgagc gtcgctctgt
    27361 gcgtaaccgc cagttacggt agaagtcgca gctacggaag tacctgcggt gaaagccaga
    27421 actgcggcca gtgctgaaag acatgcaatt tttttcataa ccacctcaaa tgtgattcaa
    27481 ataagtccta agttttaaat atatcaaaaa ttaatgggaa actcttcgcg atttgtgatg
    27541 tctaacgggc catttcatgt aacagaacgt ttccatacac cgctatccat ctaaatttaa
    27601 atcacttttt cagagaactg cgtaagtatt acgcatgttt tccctgtcat tcatccagat
    27661 tattcctaat caccagacta atgattccat caatcctggc gcattttagt caaaacgggg
    27721 gaaaattttt tcaacaaatg ctcaaccagc attgggtata tccagtacac tccacgcttt
    27781 acttaagtct agatatttgt gggagaaagg atgcctggtt cattacgtaa aatgccggtc
    27841 tggttaccaa tagtcatatt gctcgttgcc atggcgtcta ttcagggtgg agcctcgtta
    27901 gctaagtcac tttttcctct ggtgggcgca ccgggtgtca ctgcgctgcg tctggcatta
    27961 ggaacgctga tcctcatcgc gttctttaag ccatggcgac tgcgctttgc caaagagcaa
    28021 cggttaccgc tgttgtttta cggcgtttcg ctgggtggga tgaattatct tttttatctt
    28081 tctattcaga cagtaccgct gggtattgcg gtggcgctgg agttcaccgg accactggcg
    28141 gtggcgctgt tctcttctcg tcgcccggta gatttcgtct gggttgtgct ggcggttctt
    28201 ggtctgtggt tcctgctacc gctggggcaa gacgtttccc atgtcgattt aaccggctgt
    28261 gcgctggcac tgggggccgg ggcttgttgg gctatttaca ttttaagtgg gcaacgcgca
    28321 ggagcggaac atggccctgc gacggtggca attggttcgt tgattgcagc gttaattttc
    28381 gtgccaattg gagcgcttca ggctggtgaa gcactctggc actggtcggt tattccattg
    28441 ggtctggctg tcgctattct ctcgaccgct ctgccttatt cgctggaaat gattgccctc
    28501 acccgtttgc caacacggac atttggtacg ctgatgagca tggaaccggc gctggctgcc
    28561 gtttccggga tgattttcct cggagaaaca ctgacaccca tacagctgct ggcgctcggc
    28621 gctatcatcg ccgcttcaat ggggtctacg ctgacagtac gcaaagagag caaaataaaa
    28681 gaattagaca ttaattaaat ttacatttct gcatggttat gcataaccat gcagaatttc
    28741 tcgctacttt tcctctacac cgtctttata tatcgaatta tgcaaaagca tatttattcc
    28801 gaaaattcct ggcgagcaga taaataagaa ttgttcttat caatatatct aactcattga
    28861 atctttatta gttttgtttt tcacgcttgt taccactatt agtgtgatag gaacagccag
    28921 aatagcggaa cacatagccg gtgctatact tagtctcgtt aattactggg acataacatc
    28981 aagaggatat gaaattatga gtaccgctaa attagttaaa tcaaaagcga ccaatctgct
    29041 ttatacccgc aacgatgtct ccgacagcga gaaaaaagca acagtagagt tgctgaatcg
    29101 ccaggttatc cagtttattg atctttcttt gattaccaaa caagcgcact ggaacatgcg
    29161 cggcgctaac ttcattgccg tacatgaaat gctggatggc ttccgcaccg cactgatcga
    29221 tcatctggat accatggcag aacgtgcagt gcagctgggc ggtgtagctc tggggaccac
    29281 tcaagttatc aacagcaaaa ccccgctgaa aagttacccg ctggacatcc acaacgttca
    29341 ggatcacctg aaagaactgg ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc
    29401 gattggcgaa gcgaaagatg acgacaccgc agatatcctg accgccgcgt ctcgcgacct
    29461 ggataaattc ctgtggttta tcgagtctaa catcgaataa atccatcgct gatggtgcag
    29521 aactttagta cccgataaaa gcggcttcct gacaggaggc cgttttgttt tgcagcccac
    29581 ctcaacgcac ttatttagtg catccatctg ctatctccag ctgattaagt aaattttttg
    29641 tatccacatc atcacacaat cgttacataa agattgtttt ttcatcaggt tttacgctaa
    29701 ataatcactg tgttgagtgc acaattttag cgcaccagat tggtgcccca gaatggtgca
    29761 tcttcagggt attgccctat aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa
    29821 ggagttgcaa aactggcacg attttttcat atatgtgaat gtcacgcagg ggatcgtccc
    29881 gtggatagaa aaaaggaaat gctatgaagt ctgtattaaa agtttcactg gctgcactga
    29941 ccctggcttt tgcggtttct tctcatgccg cggataaaaa attagttgtc gcgacggata
    30001 ccgccttcgt tccgtttgaa tttaaacagg gcgataaata tgtgggcttt gacgttgatc
    30061 tgtgggctgc catcgctaaa gagctgaagc tggattacga actgaagccg atggatttca
    30121 gtgggatcat tccggcactg caaaccaaaa acgtcgatct ggcgctggcg ggcattacca
    30181 tcaccgacga gcgtaaaaaa gcgatcgatt tctctgacgg ctactacaaa agcggcctgt
    30241 tagtgatggt gaaagctaac aataacgatg tgaaaagcgt gaaagatctc gacgggaaag
    30301 tggttgctgt taagagcggt actggctccg ttgattacgc gaaagcaaac atcaaaacta
    30361 aagatctgcg tcagttcccg aacatcgata acgcctatat ggaactgggc accaaccgcg
    30421 cagacgccgt tctgcacgat acgccaaaca ttctgtactt catcaaaacc gccggtaacg
    30481 gtcagttcaa agcggtaggt gactctctgg aagcgcagca atacggtatt gcgttcccga
    30541 aaggtagcga cgagctgcgt gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg
    30601 gaacttacaa cgaaatctac aaaaaatggt tcggtactga accgaaataa taacgctaca
    30661 cctgtaaagc gcactggcag ttccctctcc cctctgggga gaggattagg gtgaggggcg
    30721 caaacccgct ccggggccat taattaccct gaatttgatt atttacacca cggtaacagg
    30781 aacaacatat gcagtttgac tggagtgcca tctggcctgc cattccgctt ctgattgaag
    30841 gtgccaaaat gaccctgtgg atttcggtcc tcggtctggc tggcggtctg gtaatcggat
    30901 tgctggcagg ttttgcacgc accttcggtg gttggatagc caaccacgtc gcgctggtct
    30961 ttattgaggt gatccgcggc acacctatcg tcgtccaggt gatgtttatt tatttcgccc
    31021 tgccgatggc gtttaacgac ttacgcatcg acccatttac tgcggcggtg gtcaccatca
    31081 tgatcaactc cggcgcgtac attgcggaaa tcacgcgtgg tgcggtgctg tctattcaca
    31141 aaggttttcg tgaagcagga ctggcgctcg gtctttcacg ttgggaaacc attcgctacg
    31201 tcattttacc gctggcactg cgtcgtatgc tgccgccgct gggtaaccag tggatcatca
    31261 gcattaaaga cacctcgctg tttattgtga tcggcgtggc ggaactgacc cgtcaggggc
    31321 aagaaattat tgccggtaac ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct
    31381 atctgattat taccctggtg ctaagcttta ttttgcgtcg tctggaaaga aggatgaaaa
    31441 tcctgtgatt gaatttaaaa acgtctccaa gcactttggc ccaacccagg tgcttcacaa
    31501 tatcgatttg aacattgccc agggcgaagt cgtggtgatt atcgggccgt ccggttccgg
    31561 taaatcgacc ctgctgcgct gcatcaacaa actggaagaa atcacctccg gcgatctgat
    31621 tgtcgatggc ctgaaggtta acgatccgaa agttgacgag cgcctgattc gccaggaagc
    31681 aggtatggtg ttccagcagt tttacctctt cccgcatctg acggcgctgg aaaacgtcat
    31741 gtttggtccg ctacgcgtgc gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga
    31801 gctgctggcg aaagtcggtc tggcagaacg tgcacatcac tacccttccg aactttctgg
    31861 tggtcaacag cagcgtgtgg cgattgcccg cgcgctggcg gtgaagccga aaatgatgct
    31921 gtttgatgaa ccgacttccg ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat
    31981 gcaggatctg gctgaagaag ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc
    32041 cgagaaagta gcttcgcggc tgatctttat cgacaaaggc cggattgcgg aagatggcga
    32101 tccgcaggta ttgatcaaga acccgccgag ccagcgcttg caggaatttt tgcagcacgt
    32161 ctcttaactt ctctgcctcc ctttcacccg aaagggaggc acaccagatt cctctcattt
    32221 aaaatcgccc ctcccccagc atctatactt atctttttgc tctgttttct cactggagga
    32281 gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg gcgcacctgc ccacgcggta
    32341 tccatacccg gcgttacaac cacaacgaca acggactcaa cgactgaacc ggccccggaa
    32401 ccggatatcg aacaaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac
    32461 acctcgcgta aagaattgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa
    32521 ccggtcccga agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc
    32581 accgaagtca gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc
    32641 aatatcaccg gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc
    32701 catttttcaa tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca
    32761 ctgccgctgt atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac
    32821 tggttgcagc ttccggcgat gattatcggg gcgtttatta tcgacctgct gttactggcc
    32881 ctgacattat ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc
    32941 gctttccaac aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta
    33001 ctacgcctga ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag
    33061 agcgcccgtt actggagtcg tcgcctgagc tggttaagca gtttgatagg ttatggcctg
    33121 attgtggccg tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac
    33181 gtcatcatta tgctgtgcat gaccgtctgg gcgttgtacc tgatctttcg taataaaaaa
    33241 gagattaccc agcatttgct caactttgcg gagcattcgc tggccttttt cagcctgttt
    33301 atccgcgcct ttgcgctggt gtggcactgg ctggcaagcg cctattttat cgtgctgttt
    33361 ttcttttcgt tgttcgatcc gggcaacagc ctgaaattta tgatgggtgc aacggtgcgc
    33421 agcctggcga ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggca
    33481 aaaaccatca ctctctcgcc acatactcag cgtaactatc cagagttgca aaaacggctg
    33541 aatggctggc tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcggta
    33601 atgttgctgt tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg
    33661 gggcagaaaa ccgtagatat cctgatccgt atcgcactta ttcttttttt ctcggcggtt
    33721 ggctggacgg tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc
    33781 ccgctaccca gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg
    33841 attatcagta ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca
    33901 ttgctggcag gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggtg
    33961 aaagatatca ttaccggggt gtttattcag tttgaaaacg gcatgaacac tggagatttg
    34021 gtgactatcg ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg
    34081 cgccaggata ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac
    34141 ttcgtccgcg gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct
    34201 gataaagcca atcaggcact gaaagatgcg gtagcggaat taatggaaaa cgaagaaatt
    34261 cgcgggctga ttattggtga accgaatttt gccgggattg tcggcttaag caataccgcg
    34321 tttacactgc gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc
    34381 ctcgacagcc aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact
    34441 tatcaggtgc tgcctactcc gggcgcgacc ccggctgaac cgttaccgcc gggggagcca
    34501 acgctttaac gctggcgatt gacaaaacgg cggcgctgct cgtcgttcat aaaggtccag
    34561 gcaataaagc gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg
    34621 cccacgtcgg tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag
    34681 gtaaaccaca tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag
    34741 gcgacttcac caccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg
    34801 tctttgttca gccccaggtt acggcgttta cgctcactac ctgcccgcgc tgcagcggcg
    34861 gaatcgtgga atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt atggatgatg
    34921 ccgttaaaaa ttgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc
    34981 ggattagcac tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg
    35041 gtaaaacgcc agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg
    35101 ccgatatcca gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg
    35161 gcaaggtgat gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg
    35221 atatcccagt tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc
    35281 gccagcggat tggcaaagtc tacgctttgc tccccggcgg gtgtaagcgt gaggaattgc
    35341 ctgagttcag gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg
    35401 cgcggatgca accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag
    35461 atacccgcat aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta
    35521 acatggctat ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattaattt
    35581 gtcttatttt gtaaaaatat atcgttcaaa aaataagcac atctctgtca attttttgcg
    35641 tcatttgcat cgcgcaggcg acctacactt atcaggcact acccgttgta tttatgagga
    35701 tgtcattatg aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct
    35761 cacggcttat gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg
    35821 taccgtttcg gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa
    35881 agcgcgcgaa caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat
    35941 gttcggtact gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat
    36001 gcagttttcg cctgttttgt aatcaaaaaa catcaaatat gaactcaatg taaataaatg
    36061 tatttctttt tcgcgcaatg ggtgatagaa aatcgcgcca agtgataatg cttatcaaaa
    36121 ttattatcac ttgcatgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat
    36181 gctttctcgt ggcagtgaaa atttcaacat ataagaaaaa gtcacctgca aaatggaaaa
    36241 caatcgcaat ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat
    36301 tggcatcacg cctgtggctc aggcactcgc cgccgaaggg caaactaacg cggatgacac
    36361 gctggttgtc gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa
    36421 attctcgcgt ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat
    36481 taaagatcag ggcgcaacca accttaccga cgcgctcaaa aac
//
LOCUS       DAJVXP010000027        36326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVXP010000027 DAJVXP010000000
VERSION     DAJVXP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.027"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OQS10_001306"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OQS10_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS2292383.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OQS10_001307"
     CDS             1867..1992
                     /locus_tag="OQS10_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292384.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OQS10_001308"
     CDS             complement(1989..2060)
                     /locus_tag="OQS10_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292385.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OQS10_001309"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OQS10_001309"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS2292386.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OQS10_001310"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OQS10_001310"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS2292387.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OQS10_001311"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OQS10_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS2292388.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OQS10_001312"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OQS10_001312"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS2292389.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OQS10_001313"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OQS10_001313"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS2292390.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OQS10_001314"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OQS10_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS2292391.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OQS10_001315"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OQS10_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS2292392.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OQS10_001316"
     CDS             complement(9465..9872)
                     /locus_tag="OQS10_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS2292393.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OQS10_001317"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OQS10_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS2292394.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OQS10_001318"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OQS10_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS2292395.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OQS10_001319"
     CDS             complement(11538..12197)
                     /locus_tag="OQS10_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS2292396.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OQS10_001320"
     CDS             complement(12361..12672)
                     /locus_tag="OQS10_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS2292397.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OQS10_001321"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OQS10_001321"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS2292398.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OQS10_001322"
     CDS             complement(14207..14950)
                     /locus_tag="OQS10_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2292399.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OQS10_001323"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OQS10_001323"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS2292400.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OQS10_001324"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OQS10_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS2292401.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OQS10_001325"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OQS10_001325"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS2292402.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OQS10_001326"
     CDS             20005..20088
                     /locus_tag="OQS10_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292403.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OQS10_001327"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OQS10_001327"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS2292404.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OQS10_001328"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OQS10_001328"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS2292405.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OQS10_001329"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OQS10_001329"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS2292406.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OQS10_001330"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OQS10_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS2292407.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OQS10_001331"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OQS10_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS2292408.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OQS10_001332"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQS10_001332"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OQS10_001333"
     CDS             25234..25836
                     /locus_tag="OQS10_001333"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS2292409.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OQS10_001334"
     CDS             complement(25937..25996)
                     /locus_tag="OQS10_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2292410.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OQS10_001335"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OQS10_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS2292411.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OQS10_001336"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OQS10_001336"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS2292412.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OQS10_001337"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OQS10_001337"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS2292413.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OQS10_001338"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OQS10_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS2292414.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OQS10_001339"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OQS10_001339"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS2292415.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OQS10_001340"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OQS10_001340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS2292416.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OQS10_001341"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OQS10_001341"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS2292417.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OQS10_001342"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OQS10_001342"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS2292418.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36326)
                     /locus_tag="OQS10_001343"
     CDS             complement(35716..>36326)
                     /locus_tag="OQS10_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2292419.1"
                     /translation="IGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDR
                     QLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRF
                     QNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPAT
                     LKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8701 a         9741 c         9170 g         8714 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatca
//
LOCUS       DAJVXP010000028        35780 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVXP010000028 DAJVXP010000000
VERSION     DAJVXP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.028"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..581)
                     /locus_tag="OQS10_001344"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OQS10_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQS10_001345"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OQS10_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS2292420.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQS10_001346"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OQS10_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS2292421.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQS10_001347"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OQS10_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS2292422.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OQS10_001348"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OQS10_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS2292423.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OQS10_001349"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OQS10_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS2292424.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OQS10_001350"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OQS10_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS2292425.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OQS10_001351"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OQS10_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS2292426.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQS10_001352"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OQS10_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS2292427.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OQS10_001353"
     CDS             complement(10994..11137)
                     /locus_tag="OQS10_001353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292428.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OQS10_001354"
     CDS             11138..11416
                     /locus_tag="OQS10_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292429.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OQS10_001355"
     CDS             complement(11463..13559)
                     /locus_tag="OQS10_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS2292430.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OQS10_001356"
     CDS             complement(13559..14296)
                     /locus_tag="OQS10_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS2292431.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OQS10_001357"
     CDS             complement(14293..14931)
                     /locus_tag="OQS10_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS2292432.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OQS10_001358"
     CDS             complement(15045..15287)
                     /locus_tag="OQS10_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292433.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQS10_001359"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OQS10_001359"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS2292434.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OQS10_001360"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OQS10_001360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS2292435.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OQS10_001361"
     CDS             19524..19796
                     /locus_tag="OQS10_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS2292436.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQS10_001362"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OQS10_001362"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS2292437.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OQS10_001363"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OQS10_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OQS10_001364"
     CDS             22056..22862
                     /locus_tag="OQS10_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2292438.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OQS10_001365"
     CDS             22872..23279
                     /locus_tag="OQS10_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS2292439.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OQS10_001366"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OQS10_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OQS10_001367"
     CDS             23839..24636
                     /locus_tag="OQS10_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS2292440.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OQS10_001368"
     CDS             24647..25471
                     /locus_tag="OQS10_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS2292441.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OQS10_001369"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OQS10_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27824
                     /gene="pepE"
                     /locus_tag="OQS10_001370"
                     /pseudo
     CDS             27134..27824
                     /gene="pepE"
                     /locus_tag="OQS10_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27915..29546)
                     /locus_tag="OQS10_001371"
     CDS             complement(27915..29546)
                     /locus_tag="OQS10_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS2292442.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29766..33449)
                     /gene="metH"
                     /locus_tag="OQS10_001372"
     CDS             complement(29766..33449)
                     /gene="metH"
                     /locus_tag="OQS10_001372"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS2292443.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33649..34473
                     /gene="iclR"
                     /locus_tag="OQS10_001373"
     CDS             33649..34473
                     /gene="iclR"
                     /locus_tag="OQS10_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS2292444.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34790..35780
                     /gene="espL4"
                     /locus_tag="OQS10_001374"
                     /pseudo
     CDS             34790..35780
                     /gene="espL4"
                     /locus_tag="OQS10_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9233 c         9102 g         8847 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagctaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg ggggcgggaa tactttccag ttgctgaaac
    27421 agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt
    27481 atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata
    27541 tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg caaatcaacc
    27601 cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc
    27661 gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga
    27721 tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg
    27781 ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat tgccggatgc
    27841 atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta
    27901 caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca
    27961 ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa
    28021 tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg
    28081 tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg
    28141 ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc
    28201 tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc
    28261 gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg ttcaaggttg
    28321 agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag ttcctctttt
    28381 ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc
    28441 ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc
    28501 atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc
    28561 gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc
    28621 ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg
    28681 accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc
    28741 aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta
    28801 cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc attggcggca
    28861 ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc
    28921 gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca
    28981 aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg
    29041 ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc
    29101 tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt
    29161 tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac
    29221 ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct
    29281 gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag
    29341 gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg
    29401 caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg
    29461 aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca
    29521 gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg
    29581 aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt
    29641 gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt
    29701 aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt
    29761 gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg
    29821 ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg
    29881 tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc aggccacatg
    29941 gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg
    30001 gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg
    30061 tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag
    30121 actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc
    30181 gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg
    30241 tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc
    30301 ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga
    30361 cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg
    30421 tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt cagcgttttc
    30481 tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg
    30541 cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag
    30601 aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg
    30661 ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg
    30721 cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt
    30781 acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc
    30841 agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg
    30901 tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt
    30961 aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc
    31021 gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg
    31081 agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc
    31141 agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc
    31201 accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag
    31261 gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttcccc
    31321 tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca
    31381 atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg
    31441 cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc
    31501 tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa
    31561 cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca
    31621 gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca
    31681 ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg ttcgttgcca
    31741 cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt
    31801 ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg
    31861 gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct
    31921 tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc
    31981 tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc
    32041 gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg
    32101 ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc
    32161 ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct
    32221 tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc gttttccacc
    32281 tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag gcgcttgaac
    32341 ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcttcgcca
    32401 atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg cagtttgcgc
    32461 ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag
    32521 cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg ttttgccatc
    32581 gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg
    32641 gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatcgggc
    32701 cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat
    32761 gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccagagatc
    32821 ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa taccgccgct
    32881 ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc
    32941 agcgctttgg tggactctcg ataagccacc accagcccgt caaaagtgat attacgaaat
    33001 gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg
    33061 gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc
    33121 agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc
    33181 gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag
    33241 gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct
    33301 ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga
    33361 tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc
    33421 gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta
    33481 ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc
    33541 aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattaacta
    33601 acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc
    33661 attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt
    33721 cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg
    33781 gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta
    33841 accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc
    33901 ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcgatt
    33961 gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg
    34021 cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac tcacctgatg
    34081 cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc
    34141 tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat
    34201 gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg
    34261 cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg ttgccttgca
    34321 gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt
    34381 tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag
    34441 gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt
    34501 ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa
    34561 aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc
    34621 aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc
    34681 attatgagat tgagcgattt atgatatttc tttagttgta atcatattaa attttttcac
    34741 tgttctgata gttaaaattt aagacatcaa taaacaatga gatatttaaa tgattactcg
    34801 tattcctcgt agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca
    34861 aaccctgagt gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc
    34921 gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa aatggtcgtc atatctttag
    34981 caacaaggat tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat
    35041 tacgataatt aataaaacga aaacacgaca ctcactcaaa ccattgctcc aatatttgaa
    35101 gaatacctaa tggaaatttt acctcaacgc tcagacactc ttgataaaca agaattaaac
    35161 ctaaaatcag atagaaaaga aaaagaattc ccaagaatta aacttaatgg tcaatgctat
    35221 tttccggggc gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca atatattaat
    35281 gatatttatc agaatgttga ttacaaaccc catcaagatg attattcttc agctgaaaaa
    35341 tttctcactc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt
    35401 cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt
    35461 gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat
    35521 gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt
    35581 tcggtttatg atcctaatgt aactaacgac aaaataagaa tcatgagcga aagcaaagag
    35641 gatattaaac actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg
    35701 tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag
    35761 ctattgatat taaaaggatc
//
LOCUS       DAJVXP010000029        34982 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVXP010000029 DAJVXP010000000
VERSION     DAJVXP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.029"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..815)
                     /locus_tag="OQS10_001375"
     CDS             complement(<1..815)
                     /locus_tag="OQS10_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2292445.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OQS10_001376"
                     /pseudo
     CDS             907..993
                     /locus_tag="OQS10_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OQS10_001377"
     CDS             complement(1397..1873)
                     /locus_tag="OQS10_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS2292446.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OQS10_001378"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OQS10_001378"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS2292447.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OQS10_001379"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OQS10_001379"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS2292448.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OQS10_001380"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OQS10_001380"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS2292449.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OQS10_001381"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OQS10_001381"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS2292450.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OQS10_001382"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OQS10_001382"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS2292451.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OQS10_001383"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OQS10_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS2292452.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OQS10_001384"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OQS10_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS2292453.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OQS10_001385"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OQS10_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS2292454.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OQS10_001386"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OQS10_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS2292455.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OQS10_001387"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OQS10_001387"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS2292456.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OQS10_001388"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OQS10_001388"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS2292457.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OQS10_001389"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OQS10_001389"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS2292458.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OQS10_001390"
     CDS             13848..14552
                     /locus_tag="OQS10_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS2292459.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OQS10_001391"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OQS10_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OQS10_001392"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OQS10_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OQS10_001393"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OQS10_001393"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS2292460.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OQS10_001394"
     CDS             complement(17484..18245)
                     /locus_tag="OQS10_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS2292461.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OQS10_001395"
     CDS             18378..18788
                     /locus_tag="OQS10_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS2292462.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OQS10_001396"
     CDS             complement(18750..19856)
                     /locus_tag="OQS10_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2292463.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OQS10_001397"
     CDS             complement(19867..21000)
                     /locus_tag="OQS10_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2292464.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OQS10_001398"
     CDS             complement(20993..22729)
                     /locus_tag="OQS10_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2292465.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OQS10_001399"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OQS10_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS2292466.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OQS10_001400"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OQS10_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS2292467.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OQS10_001401"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OQS10_001401"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS2292468.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OQS10_001402"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OQS10_001402"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS2292469.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OQS10_001403"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OQS10_001403"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS2292470.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OQS10_001404"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OQS10_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS2292471.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OQS10_001405"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OQS10_001405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS2292472.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OQS10_001406"
     CDS             complement(31413..31673)
                     /locus_tag="OQS10_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2292473.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OQS10_001407"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OQS10_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS2292474.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OQS10_001408"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OQS10_001408"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS2292475.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34982)
                     /locus_tag="OQS10_001409"
                     /pseudo
     CDS             complement(32997..34982)
                     /locus_tag="OQS10_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8273 a         8977 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 cc
//
LOCUS       DAJVXP010000030        34000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVXP010000030 DAJVXP010000000
VERSION     DAJVXP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.030"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            13..515
                     /locus_tag="OQS10_001410"
                     /pseudo
     CDS             13..515
                     /locus_tag="OQS10_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(582..2039)
                     /gene="nuoN"
                     /locus_tag="OQS10_001411"
     CDS             complement(582..2039)
                     /gene="nuoN"
                     /locus_tag="OQS10_001411"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS2292476.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2046..3575)
                     /gene="nuoM"
                     /locus_tag="OQS10_001412"
     CDS             complement(2046..3575)
                     /gene="nuoM"
                     /locus_tag="OQS10_001412"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS2292477.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3739..5580)
                     /gene="nuoL"
                     /locus_tag="OQS10_001413"
     CDS             complement(3739..5580)
                     /gene="nuoL"
                     /locus_tag="OQS10_001413"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS2292478.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5577..5879)
                     /gene="nuoK"
                     /locus_tag="OQS10_001414"
     CDS             complement(5577..5879)
                     /gene="nuoK"
                     /locus_tag="OQS10_001414"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS2292479.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5876..6430)
                     /gene="nuoJ"
                     /locus_tag="OQS10_001415"
     CDS             complement(5876..6430)
                     /gene="nuoJ"
                     /locus_tag="OQS10_001415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS2292480.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6442..6984)
                     /gene="nuoI"
                     /locus_tag="OQS10_001416"
     CDS             complement(6442..6984)
                     /gene="nuoI"
                     /locus_tag="OQS10_001416"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS2292481.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6999..7976)
                     /gene="nuoH"
                     /locus_tag="OQS10_001417"
     CDS             complement(6999..7976)
                     /gene="nuoH"
                     /locus_tag="OQS10_001417"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS2292482.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7973..10699)
                     /gene="nuoG"
                     /locus_tag="OQS10_001418"
     CDS             complement(7973..10699)
                     /gene="nuoG"
                     /locus_tag="OQS10_001418"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS2292483.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10752..12089)
                     /gene="nuoF"
                     /locus_tag="OQS10_001419"
     CDS             complement(10752..12089)
                     /gene="nuoF"
                     /locus_tag="OQS10_001419"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS2292484.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12086..12586)
                     /gene="nuoE"
                     /locus_tag="OQS10_001420"
     CDS             complement(12086..12586)
                     /gene="nuoE"
                     /locus_tag="OQS10_001420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS2292485.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12589..14391)
                     /gene="nuoC"
                     /locus_tag="OQS10_001421"
     CDS             complement(12589..14391)
                     /gene="nuoC"
                     /locus_tag="OQS10_001421"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS2292486.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14485..15147)
                     /gene="nuoB"
                     /locus_tag="OQS10_001422"
     CDS             complement(14485..15147)
                     /gene="nuoB"
                     /locus_tag="OQS10_001422"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS2292487.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15163..15606)
                     /gene="nuoA"
                     /locus_tag="OQS10_001423"
     CDS             complement(15163..15606)
                     /gene="nuoA"
                     /locus_tag="OQS10_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS2292488.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16235..17173)
                     /gene="lrhA"
                     /locus_tag="OQS10_001424"
     CDS             complement(16235..17173)
                     /gene="lrhA"
                     /locus_tag="OQS10_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS2292489.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18088..19305
                     /gene="alaA"
                     /locus_tag="OQS10_001425"
     CDS             18088..19305
                     /gene="alaA"
                     /locus_tag="OQS10_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS2292490.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19389..19988
                     /gene="yfbR"
                     /locus_tag="OQS10_001426"
     CDS             19389..19988
                     /gene="yfbR"
                     /locus_tag="OQS10_001426"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS2292491.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20047..21879)
                     /locus_tag="OQS10_001427"
     CDS             complement(20047..21879)
                     /locus_tag="OQS10_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS2292492.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21966..22614)
                     /gene="hxpA"
                     /locus_tag="OQS10_001428"
                     /pseudo
     CDS             complement(21966..22614)
                     /gene="hxpA"
                     /locus_tag="OQS10_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22625..23119)
                     /locus_tag="OQS10_001429"
     CDS             complement(22625..23119)
                     /locus_tag="OQS10_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS2292493.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23202..23657)
                     /locus_tag="OQS10_001430"
     CDS             complement(23202..23657)
                     /locus_tag="OQS10_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS2292494.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23995..25197
                     /gene="ackA"
                     /locus_tag="OQS10_001431"
     CDS             23995..25197
                     /gene="ackA"
                     /locus_tag="OQS10_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS2292495.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25272..27416
                     /gene="pta"
                     /locus_tag="OQS10_001432"
     CDS             25272..27416
                     /gene="pta"
                     /locus_tag="OQS10_001432"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS2292496.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27606..29126
                     /gene="yfcC"
                     /locus_tag="OQS10_001433"
     CDS             27606..29126
                     /gene="yfcC"
                     /locus_tag="OQS10_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS2292497.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29159..29701)
                     /gene="yfcD"
                     /locus_tag="OQS10_001434"
     CDS             complement(29159..29701)
                     /gene="yfcD"
                     /locus_tag="OQS10_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS2292498.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29759..30310)
                     /gene="yfcE"
                     /locus_tag="OQS10_001435"
     CDS             complement(29759..30310)
                     /gene="yfcE"
                     /locus_tag="OQS10_001435"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS2292499.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30366..31010)
                     /gene="yfcF"
                     /locus_tag="OQS10_001436"
     CDS             complement(30366..31010)
                     /gene="yfcF"
                     /locus_tag="OQS10_001436"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS2292500.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31146..31793
                     /gene="yfcG"
                     /locus_tag="OQS10_001437"
     CDS             31146..31793
                     /gene="yfcG"
                     /locus_tag="OQS10_001437"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS2292501.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31850..32212
                     /gene="folX"
                     /locus_tag="OQS10_001438"
     CDS             31850..32212
                     /gene="folX"
                     /locus_tag="OQS10_001438"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS2292502.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32233..33126
                     /locus_tag="OQS10_001439"
     CDS             32233..33126
                     /locus_tag="OQS10_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS2292503.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33174..>34000)
                     /gene="rpnB"
                     /locus_tag="OQS10_001440"
     CDS             complement(33174..>34000)
                     /gene="rpnB"
                     /locus_tag="OQS10_001440"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS2292504.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8479 a         9279 c         8565 g         7677 t
ORIGIN      
        1 gaggaaaaat aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac
       61 caactgcttg agtctactga aaaacctttg atggataata tacatgacac gctttcagga
      121 ttacgccgac tggatattga taaacgctgg gattttttac actttgggct gacaggcacc
      181 cctgcttttg atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc
      241 ctcgaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
      301 atcgaccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga
      361 ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc
      421 tttgcttcga tcatgctgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac
      481 aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc
      541 gccgaacatg tcggcggctt tctgacttac aaactgatag cttacatcag cggcattgct
      601 aaccgcacaa tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca
      661 gagatcagca ccacgatacc gcctgcgctg tactgccagt ttgacggtgc atcgcgaccc
      721 ggttgttccg gggcgtgcag gtacaggctc accgccacgc gcaggtagta gtaaaggccg
      781 cttgccgaac cgacaaccac ggcgcccacc agccaccaca agtgtgcctg gacaccgacc
      841 gccagcacgt agaacttacc aataaagccc agcgtcatcg gaataccggc cagagacagc
      901 atcatcaccg tcatcaccgc cgcgaggatc gggcgatgcc agaacagtcc gcggtaagag
      961 tacagggaat cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac
     1021 gcgccgaggc tgctaaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc
     1081 tcgccagttt gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag
     1141 taaccgagca gacgtttgat attggtctgg ctcagcgcca tcaggttacc gaagatgatg
     1201 gaggcaaagg cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc catcggtgcg
     1261 tagaggaaca gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaagaaa
     1321 gtggaaaccg gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc
     1381 agagagagtt tgaagccgag gccaacaatc atcaggccga aacctgccag caacagcggc
     1441 tcgttgagca taccgtcgcc aaggttttta cccaacgcga caaacgacag gtcgccagac
     1501 tgcgcataca ccagcgccat accaaacagc aggaaagaag acgctgcggc agaaaggatg
     1561 gtgtatttga tactggcttc cagtgaacgt ttctggcgga aagcgtaacc gaccaggcca
     1621 aacagcggca aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc
     1681 gccagcagga tcccgcccag cgcggcaatt aacaccaaca ggtagaactc gtccttgttg
     1741 tcgttatagc cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat
     1801 accagcccgg tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata
     1861 gcgcctgcct ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag
     1921 agcgtagcgt tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc
     1981 gtcaagccga cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg
     2041 gcgatttacg gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc
     2101 gcggagtgcg aggtatccag aatcggctgc ggatagaagc cctgcagtac cagcagcacc
     2161 accagcaaca ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca
     2221 atctcgcttt tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat
     2281 gcaaagacca gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg
     2341 aacagaatca taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc
     2401 gcaaagaaca gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg
     2461 cgcatgtcgc gggtatggat acgttcataa agctgaccac aaagaataaa cagacccgcc
     2521 gccgacaagc cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg
     2581 gtgtagatag caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt
     2641 ttgatatcgg tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc
     2701 cacatagcaa ttggcgcgaa ctctgccgac gcgttcggga acagcggcag ggagaaacgc
     2761 agcaaaccgt aagcggcagt tttcagcaag atccccgcga ggtcaacgga accggcggtc
     2821 ggagcctggg agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttgact
     2881 gcgaaggcga tgaagaagcc cagcattaac aggtattccg caccactgga cattggcgta
     2941 ttcagcagct cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc
     3001 agcgccagga tggcaatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg
     3061 gttgccgccg tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg
     3121 aagtacatcg gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca
     3181 aggaacacgc cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga
     3241 tatttttcga tctctttcca cgaacatagt accgccagca caccgagcag accggtcagc
     3301 acgaccatca gcagcgacag cccgtcaatg gcgagatgaa tagagatacc caaacgcggg
     3361 atccacggca tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa
     3421 taaccgccct gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc
     3481 gcgatccagc gtggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgcca
     3541 ataaagggaa ttaatattag ccagggtagt aacatggcga tctttattcc ttgtaaaagt
     3601 cccgtcagga ccggattttc aacgaattct cacgacagaa aattctcttc ggactggggg
     3661 tagtgtcgga tgcaccgcga aggcggctta tccgacctac gttcttatat gcccaggggc
     3721 aatcccacaa tccttaactc aacgcagtac catcaacagt gccagcacca cgaccgcacc
     3781 gatgctcatg gatgccacat accagcgcag atagccgttc tcacttaaca gcagaccttt
     3841 acctgcaaag cgggaaagga cagccggaat gttcatcatt gagttcagcg gatcgcgttt
     3901 cagcaaccag gcaataccca ggaacggcct gacgaacact ttgtcgtaca gccagtcaaa
     3961 gccccaggcg ttgtaccacc aggtactcag caggcggccc ggcgcactgt tggcgatgga
     4021 ggtcaccaga gtacgtttac ccagccacag ccaagctgcc agcagaatgc cgacaatcgc
     4081 gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt ccgtcgtttg
     4141 cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg aaaggatcag
     4201 cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt gaatttgttc
     4261 ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca taaacgcacc
     4321 gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg cgaggatctc
     4381 atccttactg aagaagcccg cagtgaccag cggcagtgcc gacagtgctg cgccgcccac
     4441 caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct tgaagatgtt
     4501 ctgttcgtga tggcaggcca gaatgacgga accggatgcc aggaacagca gcgctttaaa
     4561 gaacgcgtga gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc caagcgcgag
     4621 gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga tgtcggtctg
     4681 taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatgc ccaccagatg
     4741 cagaatttcc ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt agacacccgc
     4801 ggttaccatg gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg ccatcgcgtc
     4861 ggcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc ccagcagcat
     4921 cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg ccagttccac
     4981 catttcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagtg cgaaagcgag
     5041 gaaaacgtca cccacacggg tcacgacgaa cgctttcatt gccgctgcgc cattcttcgg
     5101 atcggtgtaa tagaaaccga tcagcagata ggagcacagg cccacgcctt cccagccgag
     5161 gtacatcagc agcaggttgt cggcaagcac cagaactacc atgctggcga tgaacaggtt
     5221 ggtgtaggcg aagaagcgag agtagccctc ttcaccgcgc atgtaccagg aggcgtacat
     5281 gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca ggccgtccag
     5341 caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca gcggctggct
     5401 gtatgcctgc tcgccgttag cgaagaaatc aacgccgata aaggcggtta ccagcgccgc
     5461 caggcccaca gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc gcccacggga
     5521 gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa gcatgttcat
     5581 ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg aagttgcagc
     5641 agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag aatgtacatc
     5701 acctgaccgt cggtctgacc ccagtagctt ccggcgacaa caaaggccag cgcggaggcg
     5761 ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat aaccagaccg
     5821 gttaagccaa gaatgaataa gattgccgcg aggatcagtc catgttgtaa ggggatcatg
     5881 cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc acttcacccg
     5941 cacgctcttc acgaccgacg tggaaggcca caaccagacc tgcgagcagc agcatagaag
     6001 ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct ttagcactga
     6061 ttggcgtacc gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca atcaccacca
     6121 gcatgatggc cgacaaaatt gccggaccaa tccacacctg cggtttcagc cactggcgtt
     6181 cctgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg aacagcacca
     6241 taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg cccagtgaga
     6301 agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg tgtaccggat
     6361 tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag atataaaaag
     6421 cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag gcttggcttc
     6481 gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc ggtagaagtt
     6541 atattccggg tatttgcccg gaccggagat cagcagatcc tctttttcgt aaaccagatc
     6601 ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg tggtcggaca
     6661 ggcttcttcg cacagaccac agaaaatgca gcgtgagaag ttgatgcgga aaaattccgg
     6721 ataccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga ccgggcaggc
     6781 taccgcgcag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc gggtcagaac
     6841 gatacgacca cgataacggg gcggcagata gatcggctct tccgggtaca ttcgcgtttc
     6901 gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg tgccgaaacc
     6961 tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg ccagagaatg
     7021 acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt ccagccgaag
     7081 gacattacct ggtcataacg cggacgcggt aacgacgcac gaatcaaaat gaacatcatc
     7141 ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg gccttgccag
     7201 ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat gtactcaccc
     7261 acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc cgccagttcc
     7321 tgctcggctt ccggctggtc aaacgggtga cggtgacata ccgccacgcc cgcgatggca
     7381 aaggtaataa aaccaaagaa ttgcgggata acgttccaca catgcgcctg gctgttgacg
     7441 atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga aagcccgagg
     7501 aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg cacccagcaa cgagtatttg
     7561 ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagacctgc catcatcagg
     7621 aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac tggcacaatc
     7681 gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa gatgacgcga
     7741 tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc cgcaaccagc
     7801 tggagcgaac cgccccagcc aacacggtta ggtccgtaac ggttctggaa cagacccagc
     7861 aggcgacgtt cgccaaagct catgaatgcc ccgcaggtga caaccaccag caggatcacc
     7921 accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca actcattgtt
     7981 gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaata ccggacatac
     8041 ccatcggcaa gcccacctgc cctgccgtca gcccttcagc gatttcaacc ggcagcgtga
     8101 ccgtattgcc atcgtaacta aaggagacgt gtgtacctgc gttcacacct aacttcgcgg
     8161 catccgctgg gttgagtttg atgtacggct gcggcatacg gctctggaag accggagcac
     8221 gctgtgacaa ttcatcgctg ccaaacaggt ggtaatacgg cgcgatacgc catttcccgt
     8281 cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt tcgctggttt
     8341 caaacagacg cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg tcctggaatt
     8401 tgttccacgc ctgcggggag ttccagcccg gcgcccaggc aaacggcact tgcgaacggt
     8461 gcgcagtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca atatcctgcg
     8521 gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg ctgtaacggt
     8581 gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatccggc gcagcatctt
     8641 tgatacctgc cagttccggg attttcgcca caacagcgtc aatcacatgg tcgagctgcg
     8701 tccagtccac ttcacggctc agcagggtgc tgtgcagcga gtgtaaccag cgccagcttt
     8761 ccagcatgac agttttgctg tcgtaataag caggatcgta aacctggaag aaacgttggg
     8821 cgcggccttc gttgttgatc accgtaccgt cgctttcagc aaaactggca gcagaaagta
     8881 ccagatgggc gttttccata atcgctgtgc gttgatgatc aaccaccatc accagcggcg
     8941 ctttagccag cgcagcattc acgcgggtag cggaagcgtg acgatgcaga tcgttttcca
     9001 gcaccaccac cgcgtcggcg cgtccggttt ccagttcggt caacgcttct tcaagcgaac
     9061 cgccacccat aatgcccagc cccatgctgt tgacggaacg ggcaatcatg gtgataccaa
     9121 cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga atcacctcta
     9181 tgctaccggc gttcgtcccg gagataatca acggtttctt cgcacctgcc agtgcctgca
     9241 cgatgacgtc gattttgctt tgcagctcag gttcgatacc gtcaaccgct ggcgcagagt
     9301 tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc ggtgcgcggt
     9361 aagtccacgc cgcgatatca tccagacggg tgtcatcaac gttggtaaca aacagcggat
     9421 gcttcgcacg ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc actttctgtg
     9481 ctgccgccat ttcgcgcgct ttacctttca cagcctgacg cactgccagc gcgacgcgcg
     9541 cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat tcgatttcgc
     9601 gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt tgcagacgtt
     9661 cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt tcacgcagcg
     9721 caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc ttcgactgac
     9781 gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg aaatcatcgc
     9841 cacgacgctg tactggctga cgcggacgat ccttcaggtt gacgtaaccg taaccgaaac
     9901 gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt tcgatacgac
     9961 gcagttcgcc gtaacgttca ccggggctga tgttacagcc gatggaacat tgctggcaga
    10021 tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc gttttgtcgg
    10081 taaatacgcc ggtcgggcaa atttcgacca ggttaccaga aaattcgctt tccagcgtgc
    10141 cgtcttccgg gcgaccgaag tagacgttgt cgtgcgcacc gtaaacgccc agatctgtac
    10201 cgtcagcgta atctttgtag taacgcacac agcggtagca ggcgatgcag cggttcattt
    10261 cgtgagagat gaatggcccc aaatcctgat tacggtgggt acgtttggtg aaacggtagc
    10321 gacggaagct gtgtccggtc atcacagtca tatcctgaag atggcagtta ccgccttctt
    10381 cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca ctttcacgga
    10441 actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc ggtgtcatac
    10501 aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc accgcacact
    10561 ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga atatcaaggc
    10621 ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat tctttgccgt
    10681 ctacatgaat tgtagccatt agcatgcttc cagttttctc ggtcagggac cgagcgttaa
    10741 tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt aatcaaatgg
    10801 gtattgctga acggctgttt gattcccgcc tcaaattctt cgcggaaata tttgatggcg
    10861 ctctgtaacg gctccactgc accaggtgcg tgggcacaga aagttttacc cgggcctaag
    10921 aatcgacaca gttgctcaag tgtttcgata tcgcccggct ggccttcgcc acgctccagc
    10981 gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca ccagccgcag
    11041 gactcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt gatctcatgg
    11101 tcaaccgcca tcgccagcgc cgtacccaga cggctgcccg ctttaccgat actttcgaat
    11161 tccatcggca gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc gcctggctgc
    11221 caagctttaa atttcagacc atcacgcata ccaccggcgt aatcttcgag gatctcgcgt
    11281 gcggtggtgc cgaacggcag ttcccacagt cccggatttt tcacccgacc ggagaagccc
    11341 atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc cacgccgtta
    11401 gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt cggtttgccc
    11461 catacgccgg aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg acgcccttcc
    11521 agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc ggtatggacg
    11581 aacagttcga agtcgaaacc tgttcccata atgtttttgc caagcaagcc cgcttcagtg
    11641 gcttcggcaa tggcgcggcg cagatttact gccgcttcga tatattcgcc acgcaggaag
    11701 atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc caccagcagg
    11761 tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat ttcatcggcg
    11821 ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct ccacttcagg
    11881 ccagtcgaaa agcccgctcc gccgcgccct ttcagaccag cgtcttttac ctgattaacg
    11941 atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc gtttttgctg
    12001 cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt cagcggatgc
    12061 gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca gggatcgctt
    12121 ccggggtcag atgcgcgtga gtgtcctcat cgatcatcat gtttggccct ttatcacagt
    12181 tccccaggca gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt tgccctggtt
    12241 tgatgttcag ctttttctcg agcgccgcct gaataccctg atacccgttg atatgacaga
    12301 ccacgctgtc gcaataacgg atcacatggc gaccaaccgg ctggcggaag atctgactgt
    12361 agaacgttgc cacaccttcg acgtcgcttg ccggaatacc cagcacatcg gcgatcgcgt
    12421 ggatcgcacc atccggcacc cagccacgct gcttctgaac gattttcagc gcttcaatgg
    12481 acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca cgctctgccg
    12541 cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt agcggtccac
    12601 atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca ccaggctgcc
    12661 gcggatcgcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga tacgagtgcg
    12721 ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatcccct tggtcgcctc
    12781 aatcatctgg aaagattcat tggcaggcat caccggcccc cacgacactt gcaggaagtg
    12841 ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg tggtcagcgg
    12901 gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa gaatgcgcag
    12961 actctggcgc agctcttcca ctttcagcat tacgcgggtg tagcagtcag aaacaccacc
    13021 tccaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg ccttacgcac
    13081 gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact ccagcgcctc
    13141 tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat tttgcagcgc
    13201 cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac gcagcaggcg
    13261 atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg ccgggtgcat
    13321 acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg taaaggcgaa
    13381 gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca gcaggtggct
    13441 gttgatacgg aacagttcgg agagcataac gcgaatgacg ttaacgcgat ccggcacggt
    13501 gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc agccgccgag
    13561 gtattcaata cggtcggtat acggaatgta gctgtgccag gactggcgct cgcccatttt
    13621 ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct cttcgccgtc
    13681 gagttgtaaa acgatacgga aagccccgtg cgccgacggg tggttcggac cgaggttgag
    13741 gaacatgaag tcctcgtttt cggtgccgcg cttcatcccc cactcttccg gtttgaaggt
    13801 cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg agaattcggt
    13861 agcgcgcgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg gcatcatgat
    13921 gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg tttcacgctc
    13981 ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt cgttttctgc
    14041 cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat ggtagaaaac
    14101 ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt ccatgccgtg
    14161 taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc caacttccag
    14221 taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct gaacagtaaa
    14281 ggcatccggc ccaaaacggt tgcgcagttc gccaatcacc ggatcatcaa gatgatcacg
    14341 ggtctgccag gcgggttctt gcgcggttaa gtcggtcata ttgttcacca ttgcaaatgg
    14401 tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga tttaggaata agcgaagaaa
    14461 aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt tggttacggc
    14521 aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat aaacgccctg
    14581 atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct gcaacagcat
    14641 cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca catcaaccgg
    14701 gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac cagagttggc
    14761 acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca gacgctgaat
    14821 aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag cctgacgcgg
    14881 cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg taaacgaagt
    14941 caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg agtttttacg
    15001 accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa cttcttgctc
    15061 cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct caccgttggg
    15121 atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt gacgattagc
    15181 gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg tccagtccag
    15241 cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa aaattgcagc
    15301 ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga acagatacag
    15361 cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg cggacaggcg
    15421 taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt tcgacctcgc
    15481 gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacaca ggccaatggc
    15541 aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg atgttgacat
    15601 actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg cagatggacg
    15661 ccacatcgat tcatggggag gaataaaaaa aaccttacaa tcactgtaga aattctttta
    15721 tacagctaat tgatgtggtc ttttactcct ttctataacc ttttgtcaac tttaacaaaa
    15781 gtttcttcac attagtttac ataatatcaa caccattagc atttaatgcc ctttcacgcc
    15841 agatccttga cgactccagg ataattagat gttgttgaat cgtgtccgtt gtgaagcaat
    15901 ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc caaaagagag
    15961 tattttcttg atctgtgaca cgcttttgtc attccataac aaaaacgcag caacaaattt
    16021 acgcattttt aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgttcgg
    16081 gacgtgaatc tctggtgatt gaaaaatgaa cagttttgta cgttctgcac tatgcgagga
    16141 aggattttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttac
    16201 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    16261 atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    16321 ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    16381 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtacg
    16441 ggctcatcat ttcaatcggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    16501 cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg
    16561 tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct
    16621 cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt
    16681 tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt
    16741 ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat
    16801 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    16861 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    16921 taaaacgcag tattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    16981 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    17041 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    17101 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    17161 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    17221 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    17281 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    17341 atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta agttactcac
    17401 tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac
    17461 tctcgtgcag catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat
    17521 caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag
    17581 ggtcattgcg aattatctga tgcaaagtta tgttaggcaa agtaatcttc tattattaat
    17641 aagcacatca aaaccttttt gaatattaaa taataattaa ttagcatcat cctcattcat
    17701 taattccgct taacaatagt ttcacacctt gcccccattg cgacacctcg gaaatcatca
    17761 cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta
    17821 aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttgc aattaagaat
    17881 cagaacaatt cactatataa cattgcatgc aaagcatata cgcctcatta ttttgtcatt
    17941 attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc
    18001 ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg
    18061 cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag
    18121 aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa
    18181 ggtaacaagg tactgaaact gaacatcggc aacccagccc cattcggttt tgacgcgcca
    18241 gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc
    18301 aaagggcttt actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt
    18361 gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca
    18421 atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccactc
    18481 tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc
    18541 tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg
    18601 atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg
    18661 gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac
    18721 aaaattctct acgacgatgc tgagcatcac tcaattgcgc cactggcacc tgacctgctg
    18781 accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg
    18841 atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg
    18901 gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt
    18961 ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac
    19021 cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg
    19081 ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg
    19141 gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg
    19201 ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct
    19261 ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc
    19321 cggagattgc atccggcagc gttatcccgc cacaatgacc tgatgatgtc atcatacgta
    19381 aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc
    19441 gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag
    19501 cgatggtcgc ccatgcgctg gcagctatca aaaatcgaaa atttggcggt aatgtcaacg
    19561 ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc
    19621 tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa
    19681 aaatcgctca gcaaaaactg gtcaatatgg ttccggaaga gctgcgggat atctttgcgc
    19741 cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg
    19801 cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc
    19861 tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact
    19921 tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac
    19981 cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg
    20041 ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat
    20101 ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac
    20161 cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac
    20221 gaccatcgcg aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg
    20281 agccatcaac accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac
    20341 aaacaaacac cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt
    20401 caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac
    20461 gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg
    20521 gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt
    20581 cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc
    20641 gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt
    20701 ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag
    20761 aggctcatcc gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt
    20821 cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc
    20881 aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc
    20941 gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc
    21001 cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt
    21061 cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg
    21121 ctcacgtaat ttgagatcat ccagccgttg accaatcatt ggcgatccgg ggcgaatagc
    21181 cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg
    21241 cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa
    21301 catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact
    21361 gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc
    21421 caccagcgtc atcatgccgc ttatcagccc ggcaaaactc aacggcatca tcagacgtga
    21481 cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac
    21541 gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat
    21601 tttgatttca ctgttgcccg caactttgac cagccatgtt cccattacgg tggcaacacc
    21661 ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc
    21721 agaaaagccg gaaaatactt cggggactgt cagcgttccg cttaatgcaa acgcgacaat
    21781 aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga caatggcaac
    21841 ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag
    21901 ggagatgtct tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag
    21961 gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga
    22021 ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca
    22081 atgacatgac aacccgccgc caggccagaa agcacgccag cggagcatct tccaccacca
    22141 cacactcctg cggcgcaagc cccagcagct gcgcgcctaa cagatacgca tcaggttctg
    22201 gttttccgcg cttcactcgc tcagcggtta caaacacctc tggtgcggga agcccagcta
    22261 ttttatggcg cgctcgcgct accggcatgg agccagaagt cacaatggcc cacggaatac
    22321 ctgctttatt caaatgactg agtaaggcga ttgcccccgg aagcgggtaa taccttcggt
    22381 ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt
    22441 gcccgccata aaatggcgca gagaggtgat cgcctgttta ccgtgaatga aagccagcac
    22501 ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg
    22561 cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc
    22621 ctcctcaggc attgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc
    22681 aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa
    22741 aaccgtgagt tccggcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga
    22801 cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt
    22861 catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca tgatacatct
    22921 ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca
    22981 attcacgcat ctgtaatccg taacctcgct caataattgt ttgcaggcga cggtaacgtt
    23041 cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt
    23101 gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga aaaaaaagaa
    23161 ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa
    23221 gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc
    23281 ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa
    23341 aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat
    23401 ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc aagctgaccg
    23461 cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca
    23521 taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg tttttccagt
    23581 ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga
    23641 ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca
    23701 aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt
    23761 atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata
    23821 cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc
    23881 atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta
    23941 cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg
    24001 agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat
    24061 gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct gcctgaagca
    24121 cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct
    24181 cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct
    24241 gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc
    24301 gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg
    24361 cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa
    24421 gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac
    24481 ctctacgccc tgccttacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac
    24541 ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa
    24601 gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac
    24661 ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt catgggtacc
    24721 cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc
    24781 gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg
    24841 accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca
    24901 atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat
    24961 ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc gatggttcgt
    25021 gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg
    25081 gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt
    25141 atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt
    25201 cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa
    25261 gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga
    25321 ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt
    25381 tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg
    25441 tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag
    25501 gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg
    25561 ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc
    25621 agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg
    25681 ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca
    25741 acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt tatcgttaac aaactgaacg
    25801 caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc gacgactctt
    25861 ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc cctctgccgg
    25921 ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc
    25981 gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg
    26041 tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc
    26101 tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg
    26161 gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta
    26221 aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct
    26281 ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgaac
    26341 gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc
    26401 tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc tgcgttccgt tatcagctga
    26461 ctgaacttgc gcgcaaagcg ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta
    26521 ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta
    26581 atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt gcagggattg
    26641 aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gaactgcgta
    26701 agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg
    26761 gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta
    26821 ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg
    26881 tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga
    26941 tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg
    27001 ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg
    27061 gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc
    27121 ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga
    27181 aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgtttatc ttcccggatc
    27241 tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg
    27301 ggccgatgct gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc gcactggttg
    27361 acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaacctc
    27421 gtcatcatcc gcagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt
    27481 tttttattct cttaatctgc attaatcctt tctgattatc ttgcttaact gcgctgcatc
    27541 aatgaattgc gccatttcac tttacatact taccactttg ttttgtgcaa gggaatattt
    27601 gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg cccgatacgt
    27661 tggtgattat cttttttgtt gctattttaa ccagccttgc cacctgggta gttccggtgg
    27721 ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag
    27781 tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc
    27841 gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag
    27901 gattaacctc aggatcgaaa tacgggacag ctgttggcat catcatgttt atgctggtga
    27961 ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc
    28021 ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt
    28081 cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg
    28141 caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg acctatattg
    28201 ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg
    28261 ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg
    28321 ccactatgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc
    28381 ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg gatgttgaac
    28441 aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc gtaatggtct
    28501 gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct
    28561 tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg
    28621 ttaataccat ggcttcatcc tttaccgaag ggacgcgaat gatgatcgcc cctgccctgc
    28681 tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca
    28741 gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg gacaacgcgg
    28801 tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttcttcgtg acgtccggtt
    28861 ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg gtcggtgtta
    28921 accgtcaggt taccgtgctg gcattccagt ttggtgatgg cttcagtcac atcatttacc
    28981 caacctcagc ttcgttaatg gcaactctcg gtgtgtgcag ggtggacttc cgtaactggc
    29041 tgaaggtggg cgcgacccta cttggactgc tgtttattat gtccagcgtc gtagtgatcg
    29101 gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc
    29161 attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca
    29221 gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca
    29281 gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcagc
    29341 tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg
    29401 caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca
    29461 gttgctcatc ggcctgaacc acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt
    29521 cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt
    29581 aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga
    29641 cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca
    29701 ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt
    29761 acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta
    29821 cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata ctcaccgagc
    29881 cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt
    29941 acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat
    30001 gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga
    30061 aatgcagcag catttgatcc acttcgctgt cgcagttgcc gcgcacagcg ataaccttat
    30121 gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat
    30181 tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttggg
    30241 caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca
    30301 tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt
    30361 tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc
    30421 cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg
    30481 cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc
    30541 tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact ggcttttccc
    30601 tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg
    30661 cggatgggca tcagatcgct gcgcagccag gcctgaatcc gtcgtgcacg cgcacgattt
    30721 tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac
    30781 tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg
    30841 cgtgtctgac cgtaaccttg ccacgtcggc ttcaaatgtt caccgctgtc gaggtcgatg
    30901 gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa
    30961 ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg
    31021 gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat
    31081 actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag
    31141 ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc
    31201 tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt
    31261 ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt
    31321 ctcctgccga tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc
    31381 tgactgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac
    31441 agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta
    31501 atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc
    31561 gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact
    31621 acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg
    31681 acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca
    31741 ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac
    31801 aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc
    31861 tgccgctatt attcgtataa agaaccttcg tttacgtacg tttatcggaa ttaaggaaga
    31921 agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa
    31981 agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat
    32041 tattcagcat gtagagaata accgtttctc tttgttggaa aaattaactc aggatgtgct
    32101 cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca
    32161 cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt aaccgccata
    32221 tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg
    32281 attccgcgtt tgctggagct gggccatcaa atcacggtag tgacgcgtaa cccacagaaa
    32341 gccagttccg ttctcggccc tcgggtgacg ctatggcaag ggctagccga tcaaagcaac
    32401 ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg
    32461 actcacgagc aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc
    32521 gatttgatta atgccagcga cacgccgccg tcggtattca tttccggctc ggcaacgggc
    32581 tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt
    32641 actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg
    32701 cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa
    32761 atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg
    32821 gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga taacgacctg
    32881 cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg
    32941 ctcggtcaag cgctgcatcg cccggccatt ttgcgcgtcc ccgcaaccgc cattcggctg
    33001 ttaatgggcg aatcttcaat actggtatta ggtggacaac gcgcgctgcc taaacggctg
    33061 gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt
    33121 cgctgatgtg gtttacagca aacatccgcc agttaactcc cggtgttaca ggattagtgg
    33181 ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct
    33241 aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt
    33301 gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg
    33361 cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg
    33421 taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca
    33481 atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac
    33541 agcgccattt tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac
    33601 ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag
    33661 cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat
    33721 ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcatc
    33781 atgcgaaaag ccattaattc ttccagctta ctttggtgct ctatcactac ataaatataa
    33841 cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg ccgcaggtct
    33901 tcatcaataa aactgtttga ttctagttta agcgtcgtta aatcacacag tttgcgcagc
    33961 ggcgcgggaa gatgaatatc aataaaatcc cgcgcggtgt
//
LOCUS       DAJVXP010000031        33995 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVXP010000031 DAJVXP010000000
VERSION     DAJVXP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.031"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OQS10_001441"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OQS10_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OQS10_001442"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OQS10_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS2292505.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="OQS10_001443"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="OQS10_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS2292506.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="OQS10_001444"
     CDS             complement(4834..5013)
                     /locus_tag="OQS10_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS2292507.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OQS10_001445"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OQS10_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS2292508.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OQS10_001446"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OQS10_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS2292509.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OQS10_001447"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OQS10_001447"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS2292510.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OQS10_001448"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OQS10_001448"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS2292511.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OQS10_001449"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OQS10_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS2292512.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="OQS10_001450"
     CDS             complement(10017..10259)
                     /locus_tag="OQS10_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS2292513.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OQS10_001451"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OQS10_001451"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS2292514.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OQS10_001452"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OQS10_001452"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS2292515.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="OQS10_001453"
     CDS             complement(13176..14150)
                     /locus_tag="OQS10_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2292516.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="OQS10_001454"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="OQS10_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS2292517.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="OQS10_001455"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="OQS10_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS2292518.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="OQS10_001456"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="OQS10_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS2292519.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="OQS10_001457"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="OQS10_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS2292520.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="OQS10_001458"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="OQS10_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS2292521.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="OQS10_001459"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="OQS10_001459"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS2292522.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="OQS10_001460"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="OQS10_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS2292523.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="OQS10_001461"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="OQS10_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS2292524.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="OQS10_001462"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="OQS10_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="OQS10_001463"
     CDS             27953..28156
                     /locus_tag="OQS10_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS2292525.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="OQS10_001464"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="OQS10_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS2292526.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="OQS10_001465"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="OQS10_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS2292527.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="OQS10_001466"
     CDS             31161..31433
                     /locus_tag="OQS10_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS2292528.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OQS10_001467"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OQS10_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS2292529.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OQS10_001468"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OQS10_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS2292530.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="OQS10_001469"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="OQS10_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS2292531.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8255 a         8454 c         8900 g         8386 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAJVXP010000032        32839 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVXP010000032 DAJVXP010000000
VERSION     DAJVXP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.032"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            267..1925
                     /locus_tag="OQS10_001470"
     CDS             267..1925
                     /locus_tag="OQS10_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS2292532.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OQS10_001471"
     CDS             complement(1922..2878)
                     /locus_tag="OQS10_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS2292533.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OQS10_001472"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OQS10_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS2292534.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OQS10_001473"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OQS10_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS2292535.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OQS10_001474"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OQS10_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS2292536.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="OQS10_001475"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="OQS10_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS2292537.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="OQS10_001476"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="OQS10_001476"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS2292538.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="OQS10_001477"
     tRNA            complement(9740..9827)
                     /locus_tag="OQS10_001477"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="OQS10_001478"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="OQS10_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS2292539.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="OQS10_001479"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="OQS10_001479"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS2292540.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="OQS10_001480"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="OQS10_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS2292541.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="OQS10_001481"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="OQS10_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS2292542.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="OQS10_001482"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="OQS10_001482"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS2292543.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLHDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="OQS10_001483"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="OQS10_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS2292544.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="OQS10_001484"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="OQS10_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS2292545.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="OQS10_001485"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="OQS10_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS2292546.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="OQS10_001486"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="OQS10_001486"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS2292547.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="OQS10_001487"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="OQS10_001487"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS2292548.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="OQS10_001488"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="OQS10_001488"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCS2292549.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="OQS10_001489"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="OQS10_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS2292550.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="OQS10_001490"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="OQS10_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS2292551.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /locus_tag="OQS10_001491"
     CDS             complement(28862..29488)
                     /locus_tag="OQS10_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2292552.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="OQS10_001492"
     CDS             29803..30951
                     /locus_tag="OQS10_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292553.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="OQS10_001493"
                     /pseudo
     CDS             31161..32591
                     /locus_tag="OQS10_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(32592..>32839)
                     /locus_tag="OQS10_001494"
     CDS             complement(32592..>32839)
                     /locus_tag="OQS10_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2292554.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8058 a         8030 c         8813 g         7938 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgtgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccctaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatcat
//
LOCUS       DAJVXP010000033        32596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVXP010000033 DAJVXP010000000
VERSION     DAJVXP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.033"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="OQS10_001495"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="OQS10_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS2292555.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OQS10_001496"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OQS10_001496"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS2292556.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="OQS10_001497"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="OQS10_001497"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS2292557.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="OQS10_001498"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="OQS10_001498"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS2292558.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6622
                     /gene="hemX"
                     /locus_tag="OQS10_001499"
     CDS             5417..6622
                     /gene="hemX"
                     /locus_tag="OQS10_001499"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS2292559.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            6625..7821
                     /gene="hemY"
                     /locus_tag="OQS10_001500"
     CDS             6625..7821
                     /gene="hemY"
                     /locus_tag="OQS10_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS2292560.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8483..8644
                     /locus_tag="OQS10_001501"
     CDS             8483..8644
                     /locus_tag="OQS10_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292561.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(8728..9963)
                     /locus_tag="OQS10_001502"
     CDS             complement(8728..9963)
                     /locus_tag="OQS10_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS2292562.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10110..10186)
                     /locus_tag="OQS10_001503"
     tRNA            complement(10110..10186)
                     /locus_tag="OQS10_001503"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10150..10152),aa:Pro,seq:tgg)
     gene            complement(10229..10315)
                     /locus_tag="OQS10_001504"
     tRNA            complement(10229..10315)
                     /locus_tag="OQS10_001504"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10279..10281),aa:Leu,seq:cag)
     gene            complement(10336..10411)
                     /locus_tag="OQS10_001505"
     tRNA            complement(10336..10411)
                     /locus_tag="OQS10_001505"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10376..10378),aa:His,seq:gtg)
     gene            complement(10470..10546)
                     /locus_tag="OQS10_001506"
     tRNA            complement(10470..10546)
                     /locus_tag="OQS10_001506"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10510..10512),aa:Arg,seq:ccg)
     gene            complement(10647..12032)
                     /locus_tag="OQS10_001507"
     CDS             complement(10647..12032)
                     /locus_tag="OQS10_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2292563.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12223..12963)
                     /gene="rffM"
                     /locus_tag="OQS10_001508"
     CDS             complement(12223..12963)
                     /gene="rffM"
                     /locus_tag="OQS10_001508"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS2292564.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12966..14318)
                     /gene="wzyE"
                     /locus_tag="OQS10_001509"
     CDS             complement(12966..14318)
                     /gene="wzyE"
                     /locus_tag="OQS10_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS2292565.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14315..15394)
                     /gene="rffT"
                     /locus_tag="OQS10_001510"
                     /gene_synonym="wecF"
     CDS             complement(14315..15394)
                     /gene="rffT"
                     /locus_tag="OQS10_001510"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS2292566.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15391..16641)
                     /gene="wzxE"
                     /locus_tag="OQS10_001511"
     CDS             complement(15391..16641)
                     /gene="wzxE"
                     /locus_tag="OQS10_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS2292567.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16643..17773)
                     /gene="rffA"
                     /locus_tag="OQS10_001512"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16643..17773)
                     /gene="rffA"
                     /locus_tag="OQS10_001512"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS2292568.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17778..18452)
                     /gene="rffC"
                     /locus_tag="OQS10_001513"
                     /gene_synonym="wecD"
     CDS             complement(17778..18452)
                     /gene="rffC"
                     /locus_tag="OQS10_001513"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS2292569.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18430..19311)
                     /gene="rfbA"
                     /locus_tag="OQS10_001514"
     CDS             complement(18430..19311)
                     /gene="rfbA"
                     /locus_tag="OQS10_001514"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS2292570.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19330..20397)
                     /gene="rffG"
                     /locus_tag="OQS10_001515"
     CDS             complement(19330..20397)
                     /gene="rffG"
                     /locus_tag="OQS10_001515"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS2292571.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20397..21659)
                     /gene="wecC"
                     /locus_tag="OQS10_001516"
     CDS             complement(20397..21659)
                     /gene="wecC"
                     /locus_tag="OQS10_001516"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS2292572.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21656..22786)
                     /gene="wecB"
                     /locus_tag="OQS10_001517"
     CDS             complement(21656..22786)
                     /gene="wecB"
                     /locus_tag="OQS10_001517"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS2292573.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22842..23888)
                     /gene="wzzE"
                     /locus_tag="OQS10_001518"
     CDS             complement(22842..23888)
                     /gene="wzzE"
                     /locus_tag="OQS10_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS2292574.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23900..25003)
                     /gene="wecA"
                     /locus_tag="OQS10_001519"
     CDS             complement(23900..25003)
                     /gene="wecA"
                     /locus_tag="OQS10_001519"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS2292575.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25243..26502)
                     /gene="rho"
                     /locus_tag="OQS10_001520"
     CDS             complement(25243..26502)
                     /gene="rho"
                     /locus_tag="OQS10_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS2292576.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26587..26688)
                     /gene="rhoL"
                     /locus_tag="OQS10_001521"
     CDS             complement(26587..26688)
                     /gene="rhoL"
                     /locus_tag="OQS10_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS2292577.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26829..27158)
                     /gene="trxA"
                     /locus_tag="OQS10_001522"
     CDS             complement(26829..27158)
                     /gene="trxA"
                     /locus_tag="OQS10_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS2292578.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27289..28554
                     /gene="rhlB"
                     /locus_tag="OQS10_001523"
     CDS             27289..28554
                     /gene="rhlB"
                     /locus_tag="OQS10_001523"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS2292579.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28688..30172
                     /gene="gppA"
                     /locus_tag="OQS10_001524"
     CDS             28688..30172
                     /gene="gppA"
                     /locus_tag="OQS10_001524"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS2292580.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30219..32240)
                     /gene="rep"
                     /locus_tag="OQS10_001525"
     CDS             complement(30219..32240)
                     /gene="rep"
                     /locus_tag="OQS10_001525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS2292581.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         8224 a         8806 c         8179 g         7387 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca ccgcaaggtg atactccggc
     6601 agccgcgccg caaggagaat aaccatgcta aaagtgttat tactctttgt tttgctgatt
     6661 gcggggatag tggttggccc gatgattgcc ggtcatcagg gttacgtgct gatccagact
     6721 gacaactaca atatcgaaac cagcgtcacg ggcctggcga ttattttgat tctggcgatg
     6781 gtagtgctgt ttgccattga gtggctactg cggcggatct tccgcacagg cgcacacacc
     6841 cgtgggtggt ttgtcggacg taagcgtcgc cgtgcccgta agcagaccga acaggcgctg
     6901 ctgaaactgg cggaaggcga ttatcagcaa gttgaaaagc tgatggcgaa aaatgccgat
     6961 cacgcggaac aaccggtggt gaactatcta ctggctgccg aagccgcgca acaacgtggt
     7021 gatgaagcac gcgccaacca acatctggaa cgcgcagcag agctggccgg caatgacacc
     7081 attccggtag aaatcacccg tgtacgtctg caactggccc gtaatgaaaa ccatgctgca
     7141 cgccacggcg tggataagct gctggaagtt acgccacgcc atccggaagt attacgtctg
     7201 gcggaacagg cgtatatccg cacaggtgca tggagttctc tgctggatat catcccatca
     7261 atggcgaaag cccatgttgg tgatgaagaa catcgtgcaa tgctggaaca acaggcatgg
     7321 attggcctga tggatcaggc gcgtgccgat aacggtagtg aaggtttgcg taactggtgg
     7381 aaaaaccaaa gccggaaaac gcgtcatcag gtagcgttgc aggtggcaat ggcggaacat
     7441 cttattgaat gtgacgatca tgatactgcc cagcaaatta tcatcgatgg cctgaaacgc
     7501 cagtacgacg atcgcctact gctgccgatt cctcgactga aaaccaacaa tccggaacag
     7561 ctggaaaaag tgctgcgcca gcagatcaaa aacgtcggcg atcgcccgct gttgtggagc
     7621 acactgggcc agtcactgat gaagcacgga gaatggcagg aagcatcgct cgccttccgc
     7681 gcagcgctga aacaacgtcc ggacgcctac gattacgcat ggcttgccga cgcgctggac
     7741 agactgcaca agccggaaga agctgcagcg atgcgtcgcg acggtttgat gttaacgttg
     7801 cagaacaacc caccacagta gttccttctc acccggaggc aagcacctcc gggacctttc
     7861 tgatgcataa aaaaacgcct gctcttatta cggagcaggc gttaaaacag gtctgtatga
     7921 caacagtggg tgcttcactc aacgttgtgt ccatggtgtc tgatgaggca taagcgacat
     7981 ctgtcagtgg acgataagca ccgtaaacgg ctctgcgtca ttccggagtt tatgaggcac
     8041 taaggcgaac ataagagatg gaatgagcat ctacccgttt attatgccac agagaatcgg
     8101 gaaataacat cccttaacac ttgttatgag ataattctgt aatcctcttt gcttcctgag
     8161 tgaatattta acctgagctt gatcctacac atacttatta tgaatgataa aattcattca
     8221 attaataaca catatactaa ttgccgttaa aactaaaaaa gcattaataa tcaacgcaat
     8281 ataataaacc cgccttacat atcaactgcg ccagaggtag gattgaaaac gctctcctga
     8341 ttttccaatt cattttctgg ataaataaat aatttatttt tgtcactatt atttatgtaa
     8401 tcatcctgtc agggagaggg atctcaatta tcaatgctta attacgtcat cattttgatg
     8461 acatgaaacc ggagcatttt gcatggaatt ttcgttttca cccaaacttc ttgttgttgc
     8521 tgtcgccgcc gctcttcctc tcatggccag cgcagcagat accccgtcaa ctgccaccgc
     8581 acgcaaaggc tttgccggat acgatcaccc aaaccagtat ccacagaacg ctgcaattta
     8641 ttgaatttgc acgatcatgt aggccggata aggcgtttac gccgcatccg gcaatcaacc
     8701 gcaggcggcc acaaaatggc cgctttttta cttattcacc accagcaaat gtgcctgcat
     8761 aatgtcactg gccgggcgac cgtgcgccag caaatcagcc attgctttaa gatatggcgg
     8821 tagatggcgg aaataacgct gatacccggc acacaaataa ttcagccccg gtttgccgct
     8881 ggcatcgagc atgaagcggt gtttcgggca gcctccccag cacgctttta acacgttaca
     8941 actgcgacac tgcgccggta attgcttaaa tttatcttca ccaaacacct gctgttgcgg
     9001 ggaatcgatc atttctgcaa ttgtttgctg atgcatattc cccagccgat attgcggata
     9061 aacatagtga tcgcaggcgt aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca
     9121 ggttggctga tgatggcaaa ccgcacccgg cgcaccgaca aaattggcaa acgcccattc
     9181 gatattcatc acgaaaatct tgccgacgtc gcgtttgatc cagtggtcga atatcgccac
     9241 cagaaattca ccaaattcat cggggcgcac cgaccattcc gtcagttccc cctgaatatc
     9301 accaggcgca tgcaatttca gtccatcgcg ggcagctgtt tcatcagcca gacgctcgac
     9361 caccggaata aactggatga attcgactcc cgcatcgcac aaaaaatcat aaacctgcaa
     9421 cggttgctgc gcgctggtgc ggttgacgca gaccagcacg ttatagtcga catgatgttt
     9481 ttgcagcagc gtcagggcac gcatcaccag cttatgcgtt ggtctgccac ctttggtcac
     9541 gcgatattga ttgtggatct cagccggacc gtccagcgat aacccaacaa gaaaatgatt
     9601 ttctgccaga aatgcacacc atttatcatc gagcagcacg ccgttagtct ggaagctgtt
     9661 acttatcttc ctgccagcac cgtatttcgc ctgtaacttt acggcacggc ggtaaaaatc
     9721 cagcccgagt aacgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc
     9781 gctggcagcg atatagtgac ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac
     9841 tggcttttcg cggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt
     9901 acaatcggaa ccactcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgcag
     9961 catggtcact ccttgcctga agtcatcctt caatacgtta agggcgaaat cataccgtgg
    10021 ggcaatgagt tattacgggc gtttattgcg aagggagggt gacgaaatgc acagaaaaca
    10081 aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg aacctccgac
    10141 ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt
    10201 gaatttttag ttcaattctt taaagtcgtg gtgcgagggg ggggacttga acccccacgt
    10261 ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt
    10321 cacaacttct aataatgggg tggctaatgg gattcgaacc cacgacaact ggaatcacaa
    10381 tccagggctc taccaactga gctatagcca ccactacaaa tcttgttacg cggtattact
    10441 accaccgcag ctcaagcgcc gggactaaat ggcgcgcccg acaggattcg aacctgagac
    10501 ctctgcctcc ggagggcagc gctctatcca gctgagctac gggcgcttag cgccgttgcg
    10561 gggggataat acggacttcc cacccctctg tctagtccct ttttaaataa attgcgcgtt
    10621 tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt cccgtggcga
    10681 ttaaggccaa aaactttata aatcgccgtc accgccagca taaagatgat gccaacaaac
    10741 agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat caggaatgcc
    10801 attgttacgt agttggccca cgggaacagg attgagcgga acggatggct ggcaatcgcc
    10861 gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca tggcaccatc
    10921 cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt gggaatgatg
    10981 tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc cgcaactggt
    11041 acgccgtgac gggaaacttt cgccatcgcc gccggtaact gacggttttt cgccagtgcg
    11101 tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc agccgtcagc
    11161 accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa agtcagtacg
    11221 aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat aacgaaaatc
    11281 gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacagcact gcgcagcgtc
    11341 acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc ctggtaggac
    11401 gccaccacaa tacacagcgc ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca
    11461 tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc aaagaaaatc
    11521 acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat cgcgaaccag
    11581 aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac cagcgccacc
    11641 gcgatcaatg caggtatcca ctgcgccatc tccgggaacc agaactggac ataaacgcca
    11701 atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga ccaggcggtg
    11761 agataaccaa aaaacgggct catataacga tgcgcataaa cggcgaacga accggtaaca
    11821 ggttcaagga acaacatttc gcccattgaa cgcatgatga aaaagacgaa cagcccggcg
    11881 atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc ggcccccata
    11941 aacaggccga cgccaatagt gccccccagg gcgatgagtt cgatatgtcg agcttccagc
    12001 ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt
    12061 ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag
    12121 gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa aagcagacag
    12181 gcgacggagt gaccactccg tcgctttaca aagagaggaa aatcagaggt tgccggtgta
    12241 gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag
    12301 caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg
    12361 accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg gatgcaccag
    12421 acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg tgacgatttg
    12481 cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt taaaataacc
    12541 atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt cagtttgcgc
    12601 cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc
    12661 catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct gcgggtactt
    12721 tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg cagcgttaat
    12781 taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcagcat taatggcaac
    12841 cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcgt gctgcatatc
    12901 acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg tgttgttatt
    12961 cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg aatgagtccg
    13021 gcgctttcaa aaagccagta cagcagtttt gcgatcatca gacatgcgcc gaagaccacg
    13081 ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgcgccag cacgatcata
    13141 ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg attaggctcg
    13201 cggttgccca gctcatacag ccagtcgaac catttgatga tcagaccaac cacgatcgcc
    13261 ccgagcggga tgaacaacgc gccgcccatc accaccagtg agcctataag cgtaggcgag
    13321 atcgccagtc cggagtggtt attcagcact tcccaggtaa agtagttggc tgagttcagc
    13381 accatactcg ggcgacccgg ccacagccag gaagggataa agacatagaa atcgcggaca
    13441 attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa cgccagattc
    13501 tcccacgggg agaaggtgtc gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg
    13561 ctcacattca ttccatagcg ttttagcgcc agccagaaca tgccaacaat ccccagcacg
    13621 cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat aaacaggaag
    13681 atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata agtcagcaag
    13741 ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg gcgcagaaag
    13801 tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc cacgccggag
    13861 acttcactgg agaagatctg actgtaggag ttaagccgga acagcaaaaa gccgttgtgc
    13921 atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca cgtaagattg
    13981 gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac gcgtttgcgt
    14041 aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga aagcaacgcc
    14101 tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag cacgctggtc
    14161 agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac attgaagtta
    14221 aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc
    14281 cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg gcggcaatag
    14341 ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg gtgtttttat
    14401 ccaccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag
    14461 taaacagcac cggcaaatgc tgttccgtca tatcctgcca gaacggattt tcccggttaa
    14521 gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc tgctggcggg
    14581 caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg tcaaattcca
    14641 gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc gcctgacgaa
    14701 cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc acttttaccg
    14761 tatcgccaaa ttgctgatga acggcgcgca aggcagcaat atggtcattg ctgcggtcac
    14821 cggagttccc caccagaatg gtcattttcc cttcacgttg ccgatcattc gccatcgtat
    14881 tgagagaagg gtccatccgc gtcgggaagt acagcagttc gccccgcacc tttggatgcg
    14941 ttttggcaaa aaagctcaaa tcaccgcggg tggcaaatac acagccgact cgcttttgcg
    15001 ccaggcgacg tagcgggtaa aaaagcttat atctcaagcc actggaaagc tcatacaggt
    15061 ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc agcagagcca
    15121 gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg ttagcttttg
    15181 ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc acagaaagcg
    15241 ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct
    15301 cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta tggtgaggga
    15361 tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga gtaaaaacac
    15421 gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg ccgcgcccag
    15481 tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga actggctgac
    15541 ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac caaagacata
    15601 agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc gcatagcggt
    15661 aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga cggtaaaact
    15721 cgccgccgcc actgccggta agacgaattt cagcgattta accacttccc gggtgatatc
    15781 gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg atgccgtaat
    15841 aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca cttcatccca
    15901 gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca aggtcaccga
    15961 cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt tatcccagct
    16021 gggtttcaga tagcttaacg ggatgacacc acgtttgatc aacatgatgg cggcaggaat
    16081 caccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac cgcccaaacg
    16141 gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg ataacgcatt
    16201 acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc aggcgatccc
    16261 catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac caaacagtcc
    16321 ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag
    16381 taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat catggtactg
    16441 ggcaacgtat ttggttacac cgttaaagat gccagctccg gcaagcacgc cgagcacggt
    16501 aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg acacagccag
    16561 caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg ccgtccacaa
    16621 ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca attaccgtac
    16681 gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt
    16741 tggtggtgta gcgatcttca ccgtggaact caccaaagcg ttcccccgca gggcaaccgt
    16801 gcagcggaat gtaatggaac accgccatga tttccgcttc tttcagaaag ttaatcaacg
    16861 cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc
    16921 catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct aacgcatcgt
    16981 agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct tccagttgcg
    17041 cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata tcgcgccagg
    17101 tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt tcacggatga
    17161 tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct tcaccacccg
    17221 ccgtatagtt tttggtttca tggaagctaa agcagccaat atgaccaatg gttcccagtg
    17281 cacgcccttt gtaagtggac atcacgccct gagcggcatc ttctaccaca aacagattat
    17341 gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg taatggaccg
    17401 gcacgataac gcgcgttttg tcggtgatcg ccgcttcaat cagcgtttcg tcgatgttca
    17461 tggtgtccgg gcgaacatcc acaaaaacga tttttgcgcc acgcagcaca aaggcattgg
    17521 cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg atatcgagca
    17581 gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact ttggcgctgc
    17641 caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg ccacacagtt
    17701 tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt
    17761 taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac attcgcacca
    17821 ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac ccgcaaagtt
    17881 gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc acctgcaccg
    17941 cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa agagacataa
    18001 ccgcgaatac cgccggacgc cgcacgtaaa atcagacatt gatgatcaaa ggtgccgcgc
    18061 acggcatttt caatccactg tgcataaaag cgtccgctgg cgtcaggcgc ataccacggc
    18121 gcacgaaaac ggctttgcgc aaatgcggcg ctggctaact gacgtaatgc gggaatatcg
    18181 gtctcttgtg ccactacagc accactatca ctgacattgt tcacgggtag tgccaaatca
    18241 acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc acccgtattt
    18301 gatgccgcaa ttttggcctg cacccgtgac cacggcgcta acacgtctgg cgtcaggagc
    18361 tgtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc gttttcccag
    18421 gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat attggccgta
    18481 gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga gccagccgtt
    18541 acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt ctactgtctg
    18601 cacaaaggta ctggcttcaa tcaggctgtc gtgagtgcca gtgtccagcc aggcaaatcc
    18661 gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt tgatggaggt
    18721 aatctccagt tcaccacgct ccgacggctt cacctgcttt gcgtactcca cgactttact
    18781 gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg gcttttcttc
    18841 cagcgagata gcgcggaaat tgtcgtcaaa ctccaccacg ccaaagcgtt ccgggtccat
    18901 cacctgatag ccaaataccg tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg
    18961 actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag gttcaccgtt
    19021 gaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg gctgttcggc
    19081 gtattccagc tggataccga actcactacc atcgcccagc aggcgctgga aataaccttt
    19141 atcttccggc gtagtgatga tgagaatttc gcggatatcg gccaacatca gcaccgacag
    19201 cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt
    19261 aatcggatgc aatcgggtgc cggaaccgcc cgccaggata atacctttca tgtttgcctc
    19321 cgctgaaaat tagcctttca gacctaaacg ctcgccctga tagctgccgt cctgcacctg
    19381 cttccaccag ctttcattag ccagatacca ctgcaccgtt ttacgcattc cactttcaaa
    19441 ggtttcctgt ggcagccagc caagttcacg ggcaattttc gaagcatcaa tggcataacg
    19501 cagatcatgc cccggacggt cagcgacaaa ggtgatcaag tcacgatagt gcaccacgcc
    19561 gtgcggcttg ttcggagcca gttcttccag cagctcgcaa atggtttcca caacatcgag
    19621 attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc cggtggtcgc
    19681 cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga tttgctgccc
    19741 gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca gcgggatcag
    19801 tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgataa gcgtcggcag
    19861 accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg acgcggaata
    19921 agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg agtgcaggtc
    19981 accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt tatcttccgt
    20041 cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga caatgttggt
    20101 ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg ctgccagatg
    20161 catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc gatcgcagat
    20221 atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg acatcaggtt
    20281 tccggcgtag gtcagcttat cgaccactac caccgcgtcg ctcgtttcgt tgatgatata
    20341 acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt ttctcatcag
    20401 cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc attgataact
    20461 ttaaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag cgcctcgtca
    20521 agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat gttaggctca
    20581 acaaccagag tttcgccgct atgccactgg gcgatcagtt cagcgatttc catcgccggg
    20641 ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc gattttcagt
    20701 tcactggcgc gtttatcggt agccgccagg caatcagcca ccgccgcttt cacctgatcg
    20761 ataacccaga acggtttgtg atcgttcact tcgcgcgcgg tacggataag ccgcgcctgc
    20821 tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca
    20881 gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat cagttcccag
    20941 acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc gatattcaca
    21001 tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt agtgacgaca
    21061 cactcacctt cgaggaaaat tttgtacagt tcgctggcgc gggtcgaaca aaccggcgtc
    21121 ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc cggtaacacg
    21181 cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg tgggaaagtg
    21241 agatccggac gcatctctgc taaccattct gccatcttct cggttgaccc caccggcgag
    21301 gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc
    21361 gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtggg tacagcaatc
    21421 agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca
    21481 gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg attgatggta
    21541 tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga ggcaaacgct
    21601 gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc aaaactcata
    21661 gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga ccatcaccat
    21721 acgggttatg ggcgcggctc atagtttgat attcgttttc gtcttttaaa agacgcgtca
    21781 cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg
    21841 cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc gaaggcgctt
    21901 cttcctgaat gccgcctgag tcggtcaaaa tcagccaggc gtggttcatc agccagacaa
    21961 acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc agaatgcgat
    22021 tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg atgtcctggt
    22081 gcgtggtggc aatgtctgcc aacgcgtggc agatttcttc aaagccacga ccgaaacttt
    22141 cacgcctgtg accggtcacc agaatcatct ttttatcggg gtcgataaac gggtaatttg
    22201 ccgccagttc tgaacgcagc gtatcgctgc tcatcacctg gtcacgcacc cataacagtg
    22261 catcaataac tgtattacca gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca
    22321 agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc ccggtcaatg
    22381 tacggttagc ctcttccggc cacggcgaat aaagatcgcc cgtgcgcaga ccagcctcaa
    22441 cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc gtcgtcgttg
    22501 tgtcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt tttagccctt
    22561 ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt
    22621 agtcaggtac aatggaaaag agtttcagca cctgatcgag catctcccga tgctgcgcag
    22681 tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc aacggcgcca
    22741 tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg attctcttcg
    22801 aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga gcaacggcgg
    22861 gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat catcaggaag
    22921 gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata gcgataggtc
    22981 tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc ccgattctga
    23041 tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg agcctggagc
    23101 attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg cacatctgtc
    23161 gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgttcaat actgttcatc
    23221 cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgtgc tttcatctgg
    23281 atggtacgcg ccgcccatgc gcctttcagc tcatcattca gatggctggc tgcacgctgg
    23341 ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt ttcagcaata
    23401 agcttcacgc tgtcattgac cgcgcgggta aagtctccgg ggataaactg gatgttgtta
    23461 atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg ctgtttgtaa
    23521 tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg cataacaaac
    23581 tctttatagg cttcgtccat gaccgatggt tggtcggcag aagccatgtt tgaacggaca
    23641 tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt caccgttgga
    23701 cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata cgccagcgcg
    23761 attaacgcaa atgccagccc catgccaata atccatagct tcccagccca caaggtacga
    23821 aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc aggcattggt
    23881 tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga tttctacgca
    23941 gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta atgcaatatc
    24001 catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg acaaaatgag
    24061 aatattctgc cagcacgcca atggaagcga gcagtgccgc agcaagggta atcagcacaa
    24121 acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag
    24181 gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc atatccatta
    24241 gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt ttgccctggg
    24301 tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc gcatcaccca
    24361 taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat ggcaggatgg
    24421 cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac cacaaaatca
    24481 taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca atgccatcaa
    24541 ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag taaccaaacg
    24601 gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga taaagtttgc
    24661 cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt tttacgctga
    24721 tatcaaaacg gtcatccagc gcgccaatga aaacaagcac accggcacaa gcgagataga
    24781 gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa
    24841 ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag tttggtttat
    24901 ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt gtcgtgaata
    24961 aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat gctctcagag
    25021 aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa gtccgttccg
    25081 aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata acacgccagc
    25141 aaattccagg aattttccta acatcgccag cgcggcatta agattacaga tataaaagca
    25201 aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc gtttcatcat
    25261 ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga attccattgc
    25321 atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct gcagttcttc
    25381 ctgagtagtg agcagttctt ctttacgggt accggaacgg ttgtagtcga tagccgggaa
    25441 gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg tacctttaaa
    25501 ctcttcgtag ataacttcgt ccattttaga accggtatcg ataagcgccg tcgcgataat
    25561 agtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt tcggacgatg
    25621 cagggcgttg gcatccacac caccggtcaa cactttacct gacgccggaa caacggtgtt
    25681 gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt tcttgtgctc
    25741 aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg
    25801 ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct cggttacttc
    25861 ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt tgtaagcaat
    25921 gctctgggca atgttctgca gcagcatggt tttaccggct ttcggcggtg ccacaatcag
    25981 accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag ttaaatcttc
    26041 agtagaaccg ttaccacgtt ccatacgcag acgagaattt gcgtgcagcg gggttaagtt
    26101 ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt cgttaacttt
    26161 cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag agatggtatc
    26221 accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt catcaggacc
    26281 ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca atatctccag
    26341 tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat
    26401 aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc cgagagtgat
    26461 cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt gagttcttaa
    26521 acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt
    26581 ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg cagaactgaa
    26641 acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt aagataaaac
    26701 gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata cgaagtcaac
    26761 actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca cccgaacatg
    26821 aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt agacagtgcg
    26881 cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg gataccacgg
    26941 atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc aacggtcagt
    27001 ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt gcacggaccg
    27061 caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac atccgtgtca
    27121 aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac aggaataagc
    27181 ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga ctttatttca
    27241 ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat gagcaaaaca
    27301 catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt agaagccctt
    27361 gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc gctgacgctg
    27421 gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt
    27481 acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa
    27541 ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca tgccgacgca
    27601 gaaccgctgg cggaagctac tggcctgaag ctgggtctgg cttacggtgg tgatggctac
    27661 gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac cacgggtcgt
    27721 ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt agtggttctg
    27781 gatgaagccg atcgcatgta cgatctgggc tttattaaag atatccgctg gctgttccgc
    27841 cgtatgccgc ctgcaaacca gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg
    27901 gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt ggaaccggaa
    27961 cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga agaaaaaatg
    28021 cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat tttcgccaat
    28081 accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca tcgtgtcggt
    28141 ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt
    28201 ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca tattccggca
    28261 gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt tcaccgtatt
    28321 ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg tgaagagtat
    28381 gcattgaatt tgcctgctat tgaaacctat attggtcact caattccggt aagcaaatac
    28441 aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg cccacgtaca
    28501 ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa
    28561 tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat cccgatgagc
    28621 atactgtagt aagtgattca ggtgaaagaa tgcagccaac acagagacag attgaaggat
    28681 gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt cgaatagttt
    28741 tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc gaataaaacg
    28801 caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc ctgtctaatg aagcaatgga
    28861 gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc ctccctcgca
    28921 aattcgcgtt gtcgctacgg cgacattacg tcttgccgtc aatgcgggtg attttattgc
    28981 caatgcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag aggaagcacg
    29041 tctgatttat cagggcgttg ctcacacaac tggcggtgcc gatcagcgcc tggtggtgga
    29101 tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca cctcgttgtt
    29161 cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc gtaatctggg
    29221 gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga
    29281 tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt
    29341 acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa agttgcagca
    29401 attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg acgggctgac
    29461 gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca tttttaccga
    29521 actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg
    29581 catgttgcat ctggccgtcg agcaggatat tcgcagccgt acgctgcgta atattcagcg
    29641 ccgctttatg atcgatattg atcaggcgca gcgcgtagcc aaagttgcgg ctaacttctt
    29701 cgatcaggtg gaaaaggaat ggcatcttga agcaataagc cgcgatttgc tcatcagcgc
    29761 ctgtcagctt catgaaatcg gcctgagcgt tgactttaaa caagcgccgc aacacgctgc
    29821 ttatctggtg cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa aattgctggc
    29881 gacgctactg ctcaaccaga ctaatccggt cgatctctca tcgctgcatc agcaaaatgc
    29941 cgtaccgccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcca tcatttttgc
    30001 cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc atgaactgtt
    30061 gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag agattattgc
    30121 tcaggaaagc cagtggcaga gctatgtcca ctggccgctg gaagtgcatt aatgagtaag
    30181 tgccggatgc gatgctgacg catcttttcc ggccttgatt atttccctcg ttttgccgcc
    30241 atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc ttcggcgctg
    30301 accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg
    30361 ctcggctccg ggcgcaccag ctcgccgtac tgacggcgtt ctttacacag cgtaaaggtc
    30421 aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc atcgatatta
    30481 tcttcatcga tgctgctctg gtgcggcaaa aacccttctt ccataccgac catgtagaca
    30541 taaggaaact ccagcccttt tgacgcgtgc agagtcatca gttgcacctg atccagctct
    30601 tcttcactct caccacgctc catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg
    30661 agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca gctaaacagt
    30721 tggttgacgt tcttcatgcg catttcggcg gctttcgggc tgggcgatgt ttcgtacagc
    30781 caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc
    30841 gccagacgct ggatttctgc caaccagtga gtaaagcggg tcaatgcttc ataaccacgt
    30901 ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct tttattgcgc
    30961 gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctct cttcggcgtg
    31021 ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg cagataagcc
    31081 agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat tttgtacggg
    31141 atgcggtttt gcatcaggaa tttttcaaac actcgcgact gatggttacc gcgataaaga
    31201 atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag ttcaccagtg
    31261 acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa ctttaagctc tgtgccgtaa
    31321 cccagttcgg agaacagacg cttctcaaag acgtgcgggt tattggcgat caggatgttc
    31381 gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat caccttcagc
    31441 gccggaaaat cctgactcag cagcaccagg ttttgtggac gtgcgccgcg ccaggagtag
    31501 atcgactggt cgtcatcgcc caccacggta aagcgcgcgc ggctgcccac cagcagtttc
    31561 accagttcat actggctggt gttggtgtcc tgatactcat ccaccagcag atagcgaatt
    31621 ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gcagcaacgt cggcaataaa
    31681 atcagatcat cgaagtcgag aacattgcac gctttcaggt gtgcatcata cagcccataa
    31741 caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg ggacggtgtt
    31801 ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag aactttgtca
    31861 tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt atcgtcaaac
    31921 agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat atccagcccc
    31981 agcgtgtgga aggttgagat catcagccca cgcgcctctt tgcgacccag cgtctgtccg
    32041 acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc
    32101 gcctgataac cgcaaccgcg gatcagatgg gcgattttat tggtgatcac acgagtttta
    32161 ccggaacccg cgcccgccag caccaggcag gggccggtaa cgaattcgac agcttgttgt
    32221 tggccggggt ttagacgcat aggtgtattg ctcaatcttc gaacgggggg aggattgtag
    32281 cagaatcgca tttgaatgtg accgcgtgta agccgctaac cgattggctg cggatgttta
    32341 aatcagagag ggaagaccga aaccggagtt catcgtgaat ccattagact tagaaaatat
    32401 cgggtcgtaa cgttgtgtta ttactttttc agagtttact aaggagaggt gtactcgtgg
    32461 cgattaattt cagtcctaag gttggtgaaa tactggattg caactttggt aactatcctg
    32521 ttagccagaa tggacagttc agcaccacgt attacgatgg ccgcattcct cctgaaatga
    32581 taaaaaacag gttagt
//
LOCUS       DAJVXP010000034        32372 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVXP010000034 DAJVXP010000000
VERSION     DAJVXP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.034"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1051)
                     /gene="glpQ"
                     /locus_tag="OQS10_001526"
     CDS             complement(<1..1051)
                     /gene="glpQ"
                     /locus_tag="OQS10_001526"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS2292582.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDK"
     gene            complement(1056..2414)
                     /gene="glpT"
                     /locus_tag="OQS10_001527"
     CDS             complement(1056..2414)
                     /gene="glpT"
                     /locus_tag="OQS10_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS2292583.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2687..4315
                     /gene="glpA"
                     /locus_tag="OQS10_001528"
     CDS             2687..4315
                     /gene="glpA"
                     /locus_tag="OQS10_001528"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS2292584.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQAHIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4305..5564
                     /gene="glpB"
                     /locus_tag="OQS10_001529"
     CDS             4305..5564
                     /gene="glpB"
                     /locus_tag="OQS10_001529"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS2292585.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5561..6751
                     /gene="glpC"
                     /locus_tag="OQS10_001530"
     CDS             5561..6751
                     /gene="glpC"
                     /locus_tag="OQS10_001530"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS2292586.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            6944..7846
                     /locus_tag="OQS10_001531"
     CDS             6944..7846
                     /locus_tag="OQS10_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS2292587.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7887..8690)
                     /gene="yfaU"
                     /locus_tag="OQS10_001532"
     CDS             complement(7887..8690)
                     /gene="yfaU"
                     /locus_tag="OQS10_001532"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS2292588.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8708..9997)
                     /locus_tag="OQS10_001533"
     CDS             complement(8708..9997)
                     /locus_tag="OQS10_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292589.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10054..11258)
                     /gene="rhmD"
                     /locus_tag="OQS10_001534"
                     /pseudo
     CDS             complement(10054..11258)
                     /gene="rhmD"
                     /locus_tag="OQS10_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11273..12055)
                     /locus_tag="OQS10_001535"
     CDS             complement(11273..12055)
                     /locus_tag="OQS10_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS2292590.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12275..13477)
                     /locus_tag="OQS10_001536"
     CDS             complement(12275..13477)
                     /locus_tag="OQS10_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS2292591.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13577..14119)
                     /locus_tag="OQS10_001537"
     CDS             complement(13577..14119)
                     /locus_tag="OQS10_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292592.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14398..14823
                     /gene="nudI"
                     /locus_tag="OQS10_001538"
     CDS             14398..14823
                     /gene="nudI"
                     /locus_tag="OQS10_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS2292593.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(14862..15464)
                     /locus_tag="OQS10_001539"
     CDS             complement(14862..15464)
                     /locus_tag="OQS10_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS2292594.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15772..16911
                     /gene="arnB"
                     /locus_tag="OQS10_001540"
     CDS             15772..16911
                     /gene="arnB"
                     /locus_tag="OQS10_001540"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS2292595.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            16915..17883
                     /gene="arnC"
                     /locus_tag="OQS10_001541"
     CDS             16915..17883
                     /gene="arnC"
                     /locus_tag="OQS10_001541"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS2292596.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            17883..19865
                     /gene="arnA"
                     /locus_tag="OQS10_001542"
     CDS             17883..19865
                     /gene="arnA"
                     /locus_tag="OQS10_001542"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS2292597.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            19862..20752
                     /locus_tag="OQS10_001543"
     CDS             19862..20752
                     /locus_tag="OQS10_001543"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS2292598.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20752..22404
                     /gene="arnT"
                     /locus_tag="OQS10_001544"
     CDS             20752..22404
                     /gene="arnT"
                     /locus_tag="OQS10_001544"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS2292599.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22401..22736
                     /gene="arnE"
                     /locus_tag="OQS10_001545"
     CDS             22401..22736
                     /gene="arnE"
                     /locus_tag="OQS10_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS2292600.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22736..23122
                     /gene="arnF"
                     /locus_tag="OQS10_001546"
     CDS             22736..23122
                     /gene="arnF"
                     /locus_tag="OQS10_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS2292601.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23116..23382)
                     /gene="pmrD"
                     /locus_tag="OQS10_001547"
     CDS             complement(23116..23382)
                     /gene="pmrD"
                     /locus_tag="OQS10_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS2292602.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23426..24847)
                     /gene="menE"
                     /locus_tag="OQS10_001548"
     CDS             complement(23426..24847)
                     /gene="menE"
                     /locus_tag="OQS10_001548"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS2292603.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(24844..25806)
                     /gene="menC"
                     /locus_tag="OQS10_001549"
     CDS             complement(24844..25806)
                     /gene="menC"
                     /locus_tag="OQS10_001549"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS2292604.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25806..26663)
                     /gene="menB"
                     /locus_tag="OQS10_001550"
     CDS             complement(25806..26663)
                     /gene="menB"
                     /locus_tag="OQS10_001550"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS2292605.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26678..27436)
                     /gene="menH"
                     /locus_tag="OQS10_001551"
     CDS             complement(26678..27436)
                     /gene="menH"
                     /locus_tag="OQS10_001551"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS2292606.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27433..29103)
                     /gene="menD"
                     /locus_tag="OQS10_001552"
     CDS             complement(27433..29103)
                     /gene="menD"
                     /locus_tag="OQS10_001552"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS2292607.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29192..30487)
                     /gene="menF"
                     /locus_tag="OQS10_001553"
     CDS             complement(29192..30487)
                     /gene="menF"
                     /locus_tag="OQS10_001553"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS2292608.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30566..30871)
                     /gene="elaB"
                     /locus_tag="OQS10_001554"
     CDS             complement(30566..30871)
                     /gene="elaB"
                     /locus_tag="OQS10_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS2292609.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(30926..31387)
                     /locus_tag="OQS10_001555"
                     /pseudo
     CDS             complement(30926..31387)
                     /locus_tag="OQS10_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31452..32369
                     /gene="rbn"
                     /locus_tag="OQS10_001556"
     CDS             31452..32369
                     /gene="rbn"
                     /locus_tag="OQS10_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS2292610.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7848 a         8780 c         8099 g         7645 t
ORIGIN      
        1 ccttatcagg gaaatcagta aacagcccat ttacacccgc tttgttatac agagcatcat
       61 ataactgatt cacatcagta gtgtattcag gcagtttatc cgaccgcacg gtataaggat
      121 gcactaccag tttattctgc tgagcatctt gcaccatgcc agtgagtttg atattacccg
      181 gctgcgatgt ttcctcaatc aacatatggt aatccggccc gataccgtcg gcatattccg
      241 cgacttgctt catggcacct ggcttaaaca tccagtcata gctgtaatta acccagcttc
      301 cgtccggctg tttctgctgc gtttcattcc agtcggtata ggcaatcagc tgtaccagat
      361 tgagatccat gcccatttta ggttccagct cattcttaat acgcttcagc tcatcagcat
      421 caaaacattg caaataaact ttatcgtcct taccggtgta accatatttc ttcagcactt
      481 ccagcgtttt tgccgcaata tccttccctt cctgatgatg gaaccacggc gctttgattt
      541 ctggatagat accgatattt ttcccggtag agtgatttaa cccctgaaca aattcaatct
      601 cttcttcaaa ggtgtgcccc cggaagtcgg acttacccat tgggaaacgc cccggataag
      661 tctgtacttt tttaccgttt tcaatatcga aaccttcggt aaatttcagc gacttaattt
      721 catccagcgt gaaatctatc gcgtagtaac gaccgtcttt gcgcgcccga tccgggaaac
      781 gatcggcaac atcagtaaca cgatcgagat aatggtcatg cagaacaacc agatggtcgt
      841 ctttggtcat caccaaatcc tgttccagat aatccgctcc ctgcgcatac gccatcgctt
      901 ttgctggcag cgtatgctcc ggcaaatatc cactggcacc gcgatgggcg atgactattt
      961 tttcgttgct gtccgccgcc attgcactgc ttcccatgac tatagtgctc atcatgatcg
     1021 ccatgctaag gtttttcagc ttcaatttca tgccattagc ctccgttgcg tttttgcagt
     1081 aattgttcat ggcgacgttt ttcgccaatc atcacaacaa tcaacaagat aaccgccaga
     1141 atgctgccgc caatcattac cataaagccg ccatcccagc cgaagaagtc cacggtgtag
     1201 ccaacaatcg cgctcgccgc caccgaaccg cccaggtaac caaacagccc ggtaaagccc
     1261 gctgccgtac ctgccgcttt tttcggtgcc agttccagcg catgcagacc gatcagcatc
     1321 acaggaccgt agatcaggaa gccgataaca atcatacaaa tcatatcgac ggttgggtta
     1381 cctgccgggt tcatccagta aacgatagtc gcgatggtca ccagtgtcat aaagaaaacg
     1441 ccggttgccc cacggttgcc acggaagact ttatccgaca tccagccgca cagcagagtg
     1501 cccggaatac ctgcatattc ataaaggaag taggcccagg aggatttatc cagcgcgaaa
     1561 tgcttaacct ctttcagata agtcggtgac cagtcgagga tgccgtaacg cagcagataa
     1621 acgaacacgt tggcgatggc gatataccac agcagtttgt tcggcagtac gtactgcatg
     1681 aagatttgct tcgccgtcag ctcctgttcc gctttttcgt tatagtcgtc cggataatca
     1741 tttttgtact cttcgatcgg cggcaagcca caggattgcg gggtatcgcg catcatcgca
     1801 aaggcgaata atgccaccag aatggcgcag aaagcaggca tatagagcgc cgcatgccag
     1861 tcattgaacc aggccatccc cagcaggaac agcagcggcg gaataccacc accgacgttg
     1921 tgcgcacagt tccacactga cacaatgccg ccacgttctt tctgcgacca ccagtgcacc
     1981 atagtacgac cacacggcgg ccaccccatc ccctggaacc aaccgcagag gaacaacagt
     2041 acaaacatca ccgcaatgct cgacgtcgcc catggcacaa agcccataaa caacatcact
     2101 gccgccgcca gaatcaaacc tgcgggcagg aaaacgcgcg gattcgagcg atccgatacc
     2161 gaacccatga tgaatttcga aaatccataa gcaatcgaga tccccgaaag ggcaaaacct
     2221 aaatcaccgc gtgagaatcc ctgctcaacc agataaggca tagcaagcgc aaagttctta
     2281 cgaaccaaat agtaagccgc atagccaaag aatatcccca ggaaaatttg ccagcgcaat
     2341 cgacgataag tcggatcgat ctccgcggca ggtaagcgcg ctttgtgtgg cgctggttta
     2401 aaaatactca acattgatag cctccgtggc ccgtggtctt atttatgatt aacagcctga
     2461 ttcagtgaga gaacctgccg tttcttgagt tgccgcgatg ttaagaaaac attcataaat
     2521 taaatgtgaa ttgccgcaca cattattaaa taagatttac aaaatgttca aaatgacgca
     2581 tgaaatcacg cttcactttc gaatcatgag cgaatatgcg cgaaatcaaa caattcatgt
     2641 ttttactatg gctaaatggt aaaaaacgaa cttcagaggg ataacaatga aaactcgcga
     2701 ctcgcaatca agtgacgtga ttatcattgg cggcggcgca acgggagccg ggattgcccg
     2761 cgactgtgcc ctgcgcgggc tgcgcgtgat tttggttgag cgccacgaca tcgcaaccgg
     2821 tgccaccggg cgtaaccacg gcctgctaca cagcggtgcg cgctatgcgg taaccgatgc
     2881 ggaatcggcc cgcgaatgca ttagtgaaaa ccagatcctg aaacgcattg cacgtcactg
     2941 cgttgaacca accaacggcc tgtttattac cctgccggaa gatgacctct ccttccaggc
     3001 cacttttatt cgcgcctgcg aagaagcagg gatcagcgca gaagctatag acccgcagca
     3061 agcgcacatt atcgaacctg ccgttaaccc ggcattgatt ggcgcggtga aagttccgga
     3121 tggcaccgtt gatccatttc gtctgaccgc agcaaacatg ctggatgcca aagaacacgg
     3181 tgccgttatc cttaccgctc atgaagtcac gggactgatt cgtgaaggcg cgacggtgtg
     3241 cggtgttcgt gtacgtaacc atctcaccgg cgaaactcag gcccttcatg cgcctgtcgt
     3301 ggttaatgcc gccgggatct gggggcaaca cattgccgaa tatgccgatc tgcgcattcg
     3361 catgttcccg gcgaaaggat cgctgctgat catggatcac cgcattaacc agcatgtgat
     3421 caaccgctgc cgtaaacctt ccgacgccga tattctggtg cctggcgata ccatttcgct
     3481 gattggcacc acctctttac gtattgatta caacgagatt gacgataatc gagtgacggc
     3541 agaagaggtt gatattctgc tgcgtgaagg ggaaaaactg gcccccgtga tggcgaaaac
     3601 gcgcattttg cgggcctatt ctggcgtgcg cccgctggtt gccagcgatg acgacccgag
     3661 cggacgtaac gtcagccgtg gcatcgtgct gctcgaccat gctgaacgcg atggtctgga
     3721 cggatttatc accatcaccg gtggcaaact gatgacctat cggctgatgg ctgaatgggc
     3781 taccgacgcc gtgtgccgca aactgggcaa cacgcgcccc tgtacgaccg ccgatctggc
     3841 acttcctggt tcacaggaac ccgctgaagt taccttgcgt aaagtcatct ccctgcctgc
     3901 cccgctgcgc ggttctgcgg tttatcgtca tggcgatcgc acgcctgcct ggctgagcga
     3961 aggtcgtctg caccgtagcc tggtatgtga gtgcgaagcg gtaacagcgg gtgaagtgca
     4021 gtacgcggta gaaaatttaa acgttaatag cctgctggat ttacgccgtc gtacccgtgt
     4081 ggggatgggt acctgccagg gcgaactctg cgcctgccgc gctgccggac tgctgcaacg
     4141 ttttaacgtc acgacgtccg cgcaatctat cgagcaactt tccaccttcc ttaacgaacg
     4201 ctggaaaggc gtgcaaccca tcgcctgggg agatgcactg cgcgaaagcg aatttacccg
     4261 ctgggtttat cagggactgt gtggtctgga gaaggagcag aaagatgcgc tttgatactg
     4321 tcattatggg cggcggcctc gccggattac tctgtggcct gcaactgcaa aaacacggcc
     4381 tgcgctgtgc cattgtcacg cgtggtcaaa gcgcgctgca tttctcatcc ggttcgctgg
     4441 atttgctgag ccatctgcct gatggtcaac cggtgacaga cattcacagt ggactggaat
     4501 ctttgcgtca gcaggcacca gcccatcctt acagtcttct cgaaccacaa cgcgtgctcg
     4561 atctcgcttg ccaggcgcag gcattgatcg ctgaaagcgg tgcgcaattg cagggcagcg
     4621 tggaactggc ccatcagcgc gttaccccgc tcggcacttt gcgtgcaacc tggttaagtt
     4681 cgccagaagt tcccgtctgg ccgctacccg cgaagaagat atgtgtagtg gggataagcg
     4741 ggctgatgga tttccaggcg catcttgcgg cggcttcgtt gcgcgaactg gatctttcgg
     4801 ttgaaaccgc agaaatagag ctgccggaac tggatgtcct gcgcaataac gccaccgaat
     4861 ttcgcgcggt gaatatcgcc cgtttccttg ataatgaaga aaactggccg ctgttacttg
     4921 atgcgcttat tcctgtcgcc aatacctgcg aaatgatcct gatgcccgcc tgcttcggcc
     4981 tggccgatga caaactgtgg cgttggttga atgaaaaact accttgttca ctgatgcttt
     5041 tgccaacgct gccgccttcc gtgctgggca ttcgtctgca aaaccagtta cagcgccagt
     5101 ttgtgcgcca gggtggcgtg tggatgccgg gcgatgaagt gaaaaaagtg acctgtaaaa
     5161 atggcgtagt gaatgaaatc tggacccgca atcacgccga tattccgcta cgtccacgtt
     5221 tcgcggttct cgccagcggc agtttcttta gtggcggact ggtagcggaa cgtaacggca
     5281 ttcgcgagcc gattctcggc cttgatgtgc tacaaaccgc cacgcggggt gaatggtata
     5341 agggcgattt ttttgcgccg caaccgtggc agcagttcgg tgtaaccact gatgagacgc
     5401 tacgcccgtc gcaggcaggg caaaccattg agaacctgtt tgccatcggt tcggtgctgg
     5461 gcggatttga tcccatcgcc caaggatgcg gcggcggtgt ttgtgccgtc agtgctttac
     5521 atgccgctca acagattgcc caacgcgcag gaggccaaca atgaacgaca ccagcttcga
     5581 aaactgcatt aagtgcaccg tctgcaccac cgcctgcccg gtgagccggg tgaatcccgg
     5641 ttatccaggg ccaaaacaag ccgggccgga tggcgagcgt ctgcgtttga aagatggcgc
     5701 actgtatgac gaggcgctga aatattgcat caactgcaaa cgttgtgaag tcgcctgccc
     5761 gtccgatgtg aagattggcg atattatcca gcgcgcgcgg gcaaaatatg acaccacgcg
     5821 cccgtcgctg cgtaattttg tgttgagtca taccgacctg atgggtagcg tttccacgcc
     5881 gttcgcacca atcgtcaaca ccgctacctc gctgaaaccg gtgcggcagc tgcttgatgc
     5941 ggcgttaaaa atcgatcatc gtcgcacgct accgaaatac tccttcggca cgttccgtcg
     6001 ctggtatcgc agcatagcgg ctcagcaagc acaatataaa gaccaggtcg ctttctttca
     6061 cggctgcttc gttaactaca accatccgca gttaggtaaa gatttaatta aagtgctcaa
     6121 cgcaatgggt atcggtgtac aactgctcag caaagaaaaa tgctgcggcg taccgctaat
     6181 cgccaacggc tttaccgata aagcacgcaa acaggcaatt acgaatgtag agtcgatccg
     6241 cgaagctgtg ggagtaaaag gcattccggt gattgccacc tcctcaacct gtacatttgc
     6301 cctgcgcgac gaatacccgg aagtgctgaa tgtcgacaac aaaggcttgc gcgatcatat
     6361 cgaactggca acccgctggc tgtggcgcaa gctggacgaa ggcaaaacgt taccgctgaa
     6421 accgctgccg ctgaaagtgg tttatcacac tccgtgccat atggaaaaaa tgggctggac
     6481 gctctacacc ctggagctgt tgcgtaaaat cccggggctt gagttaacgg tgctggattc
     6541 ccagtgctgc ggtattgcgg gtacttacgg tttcaaaaaa gagaactacc ccacctcaca
     6601 agccatcggc gcaccactgt tccgccagat agaagaaagc ggcgcagatc tggtggtcac
     6661 cgactgcgaa acctgtaaat ggcagattga gatgtccaca agtcttcgct gcgaacatcc
     6721 gattacgcta ctggcccagg cgctggctta aactcctttc tgatgcccgg taagcatgtg
     6781 gttaccgggc atttttgcgt acacgattcc gtgcccaatg tatgcgttgc aacgcagtga
     6841 aaattcctct gaaaacgtct cgcaaaggct gaaactggca gatgtcaaag gcctgggata
     6901 accgtaatgt cgcgtcatca taaatatcag gtgacggaca accatgaccg aatcaacaac
     6961 ctcctccccg catgatgcgg tatttaaaac ctttatgttc acacccgaaa ccgcacggga
     7021 ttttctcgaa atacatttgc cagaaccact gcgcaagctt tgcaacctgc aaaccttacg
     7081 cctggaaccc actagtttta ttgaaaaaag tttacgcgct tactactcgg atgttttgtg
     7141 gtccgtggaa accagcgacg gtgacggcta tatctactgc gtgattgaac atcaaagctc
     7201 tgcagaaaag aatatggctt ttcggctaat gcgctatgcc actgccgcca tgcagcgtca
     7261 cctggataaa ggctatgaca gagttccgct ggtggtgcca ttgctgtttt atcatggcga
     7321 aacatcgccc tacccgtact cacttaactg gctggatgag tttgacgatc cgcaacttgc
     7381 ccggcagttg tacaccgaag cttttccgtt ggtggatatc accatcgtac ctgacgatga
     7441 gatcatgcaa catcggcgta tagctctgct tgaactgatt caaaagcata ttcgcgaccg
     7501 cgatttaatc ggtatggtcg acaggatcac cacgcttttg gttaaaggct tcactaatga
     7561 cagccagcta caaacactgt ttaattatct gctgcaatgc ggcgatacct cccgtttcac
     7621 ccgttttatt gaggagattt ccgaacgttc accactacaa aaggagagat tgatgactat
     7681 tgctgagcgg ctacggcagg aaggacacca aattggctgg cagaaaggta tgcatgaaca
     7741 agccattaaa attgctttgc gcatgctgga acagggcatt gatcgtgacc aggtgctcgc
     7801 ggccacccag ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca
     7861 cagccgatcc ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg
     7921 cctgatttaa acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg
     7981 ccaacagcga caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca
     8041 gccagaaaac cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt
     8101 cgctgcactt ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct
     8161 ggtccaataa acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt
     8221 gccgttttac tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa
     8281 ttctcaatgc gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg
     8341 tagggaggat agcgcgtggc agacgccacc tgacgagcct gatcggcagt atcgaccatc
     8401 gggatcaata gagtttgcgc gccaatatcc agaacttgtt taatcagcga tttactgcct
     8461 tccaccggac ggatcacggg ttggctggca tagggcgcta ccgcctgtag ctggtgataa
     8521 agatcctgaa tggtgtttgg cgcgtgctcc ccgtcaatca gcaaccagtc ataaccggaa
     8581 gtagcggcaa tttctgccat ataagaagta gtcgagctta accacagacc aatttgcact
     8641 tcgcccttgc gtaaacgttc tttaaaggga ttggttaata atgcgttcat cgtgttcctt
     8701 aacctattta atgatgtgcc acgtcggtct gcgcaacagt acgatttaca cgcagagtga
     8761 aaataatcag cgaaccgatg accgccaccg ctgccagtgt cagtaatcct gccgcatcgc
     8821 tggcaaataa cgtttctgct ttcacgcgca ggatcggggc aataaacccg cccactgcac
     8881 caaacaggtt tacaaaacca atccccgcag ccagcgccgt accggaaagc agttgtgtcg
     8941 gcatcgtcca gaacaccggc tgcacggcaa taaacccgat agctgcaaca cacagcgcta
     9001 cgatcgccat tactggagaa agcagcccgg acagaccaat accaatgccc gccgccagta
     9061 atgtcagcgc tgcgacatta cgccgttcgc cggttttatc ggaatagcgc ggaataagcc
     9121 aggtcccaaa caaggccgca acccacggaa tggcggtgac caccgacgct gtaaagccca
     9181 cttttgttcc cagcaatgcc gcaacctggg tcggcaggaa gaaaattaat ccgtaaaccg
     9241 ccacctgaat ggtcaggtag ataatcgcta gttgccagac tcggccatta cgcagcgcat
     9301 cgctcagccg agaagtcact ttctgttgtt cttcacttgc cagttgattg ataagcaacg
     9361 ttttttcttg tttactcaga aaacgtgcct gctccggtgt gtcatcaagc caaaagaatg
     9421 tgaatacccc agcaccgact gccaacaatc cttcaatcac aaacatccag aaccagccgg
     9481 gatgccccat aaatccatgc atctccaaca gcgcgccgga aagcggtgat cccagtgtta
     9541 acgccagcgg tgcgcccata tagaacagcc ccataatgct ggcgcgatta cgctgcggaa
     9601 accattgcga ggtgagataa atcataccag ggaaaaatcc ggcctccgca gcaccaagca
     9661 gagtgcgaac tatcagaaat ttcgcttcag tatcggccca tgccatggct gccgaaagaa
     9721 atccccacag cagtgttgtc gtaccaatcc aggttctggc ccccagtttg cgcatcaaaa
     9781 gattcgccgg aacacccaga aacgcatata ccacaaagaa aatgcctgct cccagcgcat
     9841 aagcttcatt actcaacccg gtatcaatct ggtaggtctg tttggcaaaa ccgatattcg
     9901 aacggtcgag aaacgccagc acatacagcg ccaacataaa cggaattaaa cgcacacggt
     9961 ttttcttcac cacggcgtca agcaaagcgg tgctcataaa aagctcctta gaatatgtgg
    10021 gcgttaccgt tgtcacagca acgcccggag tgattagtgg ctgtaggggc gtttcaaatt
    10081 gcagtcacga ttgagttcga cgccaaaacc gggtttatca agcactgatt tatgaatacg
    10141 accattcacc ggaaccggct cattgagcag aatcgggtca aactgcggac gcatcgttga
    10201 acaatccggg ctggtcatca ggaattcgct gaatggcgta ttggtgaagg tgatcaccgc
    10261 atggtgagag taaacagacg aaccgtgcgg caccaccagt tgccccctgg atttggcgat
    10321 tgcggcaatt tccaccagcg tggttaaacc accgcaccag ccaacatccg gctgcataat
    10381 gtcgataccg gtttctgaaa gcgtacgaaa agattgcagt gtgccgtggt gctcaccgct
    10441 ggtgaccatc attccgactg gcgcgttgcg tttcagttcg cgataacctt catactgctg
    10501 tggcggcagg cactcttcga tccatttcag gttatagggc gcgcaagcgt gggccagttt
    10561 ggtggcatag ttcacgtcct gactcatcca gcagtcgagc attaaccaga aatcctcacc
    10621 gcatttttca cgcatatccg cgaccataac ggcatctttg cggatccccg catcgccatc
    10681 atgtggcccc cagtgcgtcg gcattttgcc accgataaag cccatctctt ttgccagatc
    10741 cggacgcgcg cctgtggcgt agaactgaat ctcatcacga actgcgccgc ctaaaagttt
    10801 ataaaccgga agcccgacca ctttgccgaa cagatcccac agagccagat cgacacagga
    10861 aatcgtgttc atcaccaggc caccagagcc ggagtaatac agggtggcat tgagcatttg
    10921 atcatggatc agtttgatat cactgacaca tttaccctca atgaaacggt taagatgttt
    10981 ttcgacaata aaacagccca tttcaccggc tgtcgaaaca gcgaatccgg tctggccgtt
    11041 ttctgcttcg acttcaacaa tcaacgtgcc aagaacgtta atgccaaatg actggcgtga
    11101 ctgctcgtaa tcgcggtatt tactcatcgg ggtggcaata tgatcgtcaa tccagtgatt
    11161 cgccccctgg tcgtgataat caccgcgcca gcaccttttt ctgctgtcgc accgccagta
    11221 aaccaggcgc gaacctgttt gatttttggt agggtcatga tgttctccat tgttatgagg
    11281 cctgtaagtc aaagggactt ttccatccca acagacgtga aatatccctg gcgcaggcaa
    11341 tggccttgcc cgccagataa tcacggtatt cttcattgat ttgtaagcgg gtaccgacca
    11401 ccgagatcgc agcggtaagc tcgttattgg cgttaaacac cggcgcagcg acacaacgga
    11461 catcggggta atcttcgccg ttgtcatagc tccagccctg acggcgaata cgcgccagtt
    11521 cttcgtgaag ttgctgtgga tgagtaatcg ttgtgggtgt cgcctgctcc cagaccagcc
    11581 cttcgataat actttgctgt accgctgcag gttgccaggc aagcaggcat ttacctattc
    11641 cggagcgata aagcgaaagg ctttttcctt catgggaacg cacgctgata gtggctgatg
    11701 actccacttt taaaatgtaa taagcgctgc cgttatcaat aatccccagg tgacataaca
    11761 gcccggtggt atccatcagt tgcgtcaaac gcggtcgagc cagttcccgg agatccatct
    11821 tgcttaacgc atgaccagaa agctccacca gcctggtcca caaacaaaaa ttttcctggt
    11881 gatcaaggct aagaaaacgc tgacgcctga gttcattaag cagcaaatag gccgtgcttt
    11941 tagggattcc caatgtgtca atgatcgtcg cagcactaca ggggccaatg cgggcaatca
    12001 aattgagaat atcaatagcc cgtgtcaggg cgggaacttt gcttgattcc aacatactgg
    12061 actccagtct tagatactgg aatcagtctc gttgctcagg gggagtaaaa ttgtgaagcg
    12121 catcaaatcc cgctcctcgt ccagcggact ggagaaatta cgcacaaatt tgtgaatcag
    12181 tgcacggatt ttttcttatt gagtacaaca ggttggacaa gttttgccgg agggggaatg
    12241 tgccggtgca gtaaaccggc acagagtggc ggaatcagac agataaggtc atggactcaa
    12301 caacctcaat ccagccatgc tcactggcga tatcctggcc gtttaaccaa cggcgcagca
    12361 tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag cgtgtagtgc
    12421 tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag ttgagatgct
    12481 cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc cgccgttctg
    12541 taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaaccact tcacacgcca
    12601 gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg aattgctcgc
    12661 tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg ggcagtcctt
    12721 cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc cacagttttt
    12781 ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc ggcattgagg
    12841 agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt tgtgccagat
    12901 cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcgactgt ggtaaagaaa
    12961 aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat tctgacggta
    13021 cgccgggagt aaagaacatc aggcaacgat taagccgcac ggcaaaacca caggcggtgc
    13081 ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta cggttgctcg
    13141 gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc caggcttcat
    13201 gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcacttaaa tcatcactgg
    13261 tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt tcgcgaagaa
    13321 tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat ggcaacccct
    13381 gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc ccgtgtaaca
    13441 cttcatcccc ggtggataac atttccactt ttaacatggt tatctcctgc ttttgttatc
    13501 gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatggc gcgacagaat
    13561 gccgcgccgg aagggatcag aaactggcgt taaccccaac atacgggccg tcagccacgg
    13621 cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc gcctcaatac
    13681 tgactggatg cataatggtg taacgcgcgc cagcattcgc ttcctcataa cttttaatac
    13741 cgctggagag tgaatccgga gagtagtaat actcgccaaa caaacggaag ctgttgccaa
    13801 ttttccactg caaaccacct cctaccgctg cggcataacc ttcatcgccg taattcgggt
    13861 tggtgtaaac gccttttccg ccaacggtcg ccattaacgg cccgagaggc agattcaacc
    13921 ccagccccac gccagcgacg tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaacg
    13981 ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct ttacccgcct
    14041 gaccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg gccagacctg
    14101 ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc
    14161 tgcgggattg tactgcctgg cacagaggat tcaatgagcg aaaaaaggga tctagtcact
    14221 tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac actttaccga
    14281 tacacacaaa aataataata agtgcagtta tcatcgcaaa agagattact gtcactttca
    14341 catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa tacccctgtg
    14401 cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa
    14461 atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg cggcgtggag
    14521 tctggcgaac gaattgaaga ggcactacgc cgcgaaattc gcgaagaact gggagaacag
    14581 ctgcttttga cagaaatcac gccgtggacc ttcagcgatg atattcgcac caagacgtat
    14641 gcagatggtc gcaaggaaga gatttatatg atttacctga tttttgactg cgtttctgcc
    14701 aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa acctgaagat
    14761 ctggtgcatt atgatttaaa tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt
    14821 taacaatgat gacagccatc actggctgtc agattgaaaa tttagtggtt aacgaattcc
    14881 ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag ataatcaggt
    14941 ttaaatgtcg catcacgctt atttttagca atatatgtca ggcaatgatt atgggtgaaa
    15001 ataacgatat ttttatcagg cgctttgctt tgtaagtcct taattgcact ataaatctca
    15061 ttaccgcact gaagaagtcg tttatctacc gtcagttttt tacccgcgga aaaccaggtc
    15121 gccgactgaa tggtccggac ggtattactg gaataaagat cgaaatcagg gatgtcagcg
    15181 ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat acctgtttta
    15241 tctgacaagc attgattggt tgaacgatcg caacgttcag catggcgaaa caaaacgaca
    15301 accgggtgct gctgtgccag tctggccaat gttttattgt cgatacgtgg caaaccattg
    15361 ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag taaaatgatg
    15421 aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac tccacctata
    15481 gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga taattagcag
    15541 ttggtgtaat attaaaaatc ctacgatgtc ggcaaaatgc ctcaaaattt tgccaaatgc
    15601 aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat tagtttctta
    15661 aggttaagtt aatattctat ccttaaaatt tcgctccaaa tggcaaaata tacacaacac
    15721 tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa
    15781 tttttgcctt tttcgcgacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt
    15841 ctcgaatccg gttggatcac aaccggtccg aagaatcagg cgcttgagca agccttttgc
    15901 cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc
    15961 acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg
    16021 gtttcaaccc tcaatatgat ttccttgttg ggtgcaacgc cggtaatggt ggatgtcgac
    16081 cgcgatacgc tgatggtcac gcctgaagcg atcgagtcag ccattacgcc acgcactaaa
    16141 gccatcattc cggtgcatta tgccggtgcg ccagcagata ttgacgccat tcgcgccatt
    16201 ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtcgg tacgtattac
    16261 aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat
    16321 attacctgtg cggaaggtgg cctgattgta actgataatg aaaaccttgc ccgccagcta
    16381 cggatgctga aatttcacgg tctgggtgtc gatgcctatg acagacaaac ctggggccgt
    16441 gcaccgcagg ctgaagtctt aacaccgggc tataagtaca atctgaccga tattaacgcc
    16501 gcgattgccc tgacacagtt agcaaaatta gagcacctca acacccatcg gcgcgaaatt
    16561 gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc
    16621 tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt
    16681 atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gcattggtac cgggttacat
    16741 ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg
    16801 aataccgaat ggaatagcga acgcatctgt tctttgccgc tgttcccgga tatgactacc
    16861 gccgatgccg accgtgtcat cacagccctt cagcaactcg caggacaata agccatgttt
    16921 gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc
    16981 ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag
    17041 atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatgctggt cgaagcctca
    17101 caagcggaga acagccatat tgtgtctatt ttgcttaacc gcaattacgg gcaacattca
    17161 gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat
    17221 ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac
    17281 gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag
    17341 atgattaaca ggcttattca gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg
    17401 ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga acgtagcacc
    17461 tttatcccga ttctggcgaa tatcttcgcc cgccgtgcca ttgaaattcc agtacatcat
    17521 gccgagcgtg agtttggtga atccaaatac agttttatgc gcctgattaa tttgatgtac
    17581 gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagtctgct cggcagcatt
    17641 attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga
    17701 ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc
    17761 gctcagttta tcggcatggg attgctcggt gaatatatcg gcaggatcta caccgatgtc
    17821 cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa
    17881 taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagctctgc
    17941 tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc gctgaaaaag
    18001 ccttttatgg ttcggtggct cgtctggcgg cggaaagagg cattccagtt tatgcgccgg
    18061 ataacgttaa tcatccgctg tgggtggaac gcattgccca actctcacca gaggtgattt
    18121 tctcttttta ttatcgccat cttatttgcg acgagatttt gcagctcgct cccgcaggtg
    18181 catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg
    18241 tgctggtgaa cggtgaaacg gaaacgggcg ttacattgca ccgaatggta aaacgtgccg
    18301 atgctggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat atcgctatta
    18361 cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca ttacccgcca
    18421 ttaaacacgg taatattctg gaaattgccc agcgcgaaaa cgaagccacc tgttttggtc
    18481 acagaacgcc agatgacagt ttccttgaat ggcataaacc ggcatccgta ctgcacaaca
    18541 tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc aatcagaaat
    18601 tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg gggagcgtga
    18661 tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc gtcaccggac
    18721 aggcgggcga cggcattact atgcagggct cgcaattagc gcagatgctg ggcctggtgc
    18781 aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc cgggtactca
    18841 tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg cgcgaagatc
    18901 attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt ctgaatcatc
    18961 cgcattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt gagtatcacg
    19021 tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt gaatataccc
    19081 gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt atccgctact
    19141 gcgttaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat gggatgtgta
    19201 gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg aataaaccac
    19261 gctggattta ttcggtgtca aaacaattac ttgatcgggt gatctgggcc tatggcgaaa
    19321 aagagggttt acagttcacc ctcttccgcc cgtttaactg gatgggacca cgactggata
    19381 accttaatgc agcgcgaatt ggcagctccc gcgctattac gcaactcatt ctcaatctgg
    19441 tagaaggttc accaattaag ctaattgatg gcggaaaaca aaaacgctgc tttactgata
    19501 tccgggacgg tatcgaggcg ttgtaccgca ttatcgaaaa tgcgggaaat cgctgcgacg
    19561 gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa ctgggcgaga
    19621 tgctgctggc gagcttcgaa aaacatccgc tgcgccatca cttcccgccg tttgcgggct
    19681 ttcgtgttgt cgaaagtagc agctactacg gcaaaggata tcaggacgta gagcatcgta
    19741 aaccgagcat ccgcaatgcc cgccgctgcc tggactggga accgaaaatt gatatgcagg
    19801 aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg gataaaccat
    19861 catgaccaaa gtcggcttac gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt
    19921 gccacgtctg ctggaaatct tgagtaagca taatattcag gccagcattt ttttcagcgt
    19981 cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt ttttgtggaa
    20041 gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg caggtacggc
    20101 ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag cggctaaaca
    20161 tcacgaagtc ggcctgcacg cctgggatca ccatgcctgg caagcccgta gcggtaactg
    20221 ggatcggcaa acaatgatcg acgatattgc acgcggtctt cgcactctgg aagagattat
    20281 cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccaga aggtgatcga
    20341 agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca tgccgttccg
    20401 tccactgctc gaatcaggaa accctggcac tgcgcaaatt ccggtgacct tacccacctg
    20461 ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt tactcaaccg
    20521 catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg aaggctgcgc
    20581 ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg tgacattttg
    20641 ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc gcggaaatat
    20701 tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg
    20761 gtacgttacc ttatcggcct cttcgcgttt attgcctgct attacctgtt accgatcagc
    20821 acgcgtctgc tctggcaacc cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg
    20881 gcatccggcg actggattgt gccccatctg ttagggctac gttatttcga aaaacccatt
    20941 gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa ctttggtgtg
    21001 cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg gtttactctg
    21061 cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct ctcattgttt
    21121 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    21181 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    21241 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    21301 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    21361 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    21421 ctggcgatag cgcagcggga gcctgacttc tggcactatt ttttctgggt tgagcatatt
    21481 caacgctttg cactggatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    21541 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    21601 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    21661 tttttctccg tcgctaaagg taaattgccc acctatattc tttcctgctt tgcatctctg
    21721 gcaatgctga tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    21781 attaatggct ggattaacat cgcttttggc ttcactggca ttattgccac atttgtggtc
    21841 tccccgtggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    21901 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    21961 gaaaagacct ggtcttttgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    22021 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    22081 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    22141 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    22201 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    22261 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    22321 gatatcaaca gtctcgccat tccgcccgca gatgccatcg atcgtcagga gcgtctggtg
    22381 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    22441 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    22501 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    22561 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    22621 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    22681 ggtgtggggt ggcgttcatt attggcggca ttgtgatcct cgggagtacg gtgtaatggg
    22741 cctgatgtgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    22801 tgcggcgagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    22861 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    22921 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    22981 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    23041 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    23101 cacgacaaaa caacgttact gagttttccc tgccacttta cactgccgtt cccactcatc
    23161 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    23221 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    23281 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    23341 atcttgttta ttgcagcacg attttttgac cagccattcc attgcattat cctgtttgct
    23401 aagagttttc acatcaattt acagattaac agataaacct attatcaacg gcaatggaat
    23461 ggcaaaattg cgaagtgaaa ctaatgattt cgtaatcctg gcgttgcacc cactctttta
    23521 acgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggtaaa gttagccagc
    23581 gcaccggttg ttgaaaacga gccagcttat ctttcaccca ttcgccaaga tcaacggttt
    23641 gctggtcata ctccaccacc gctaccggtc gatgaccaaa ctccttgtcg gcaacgggga
    23701 cgataaacac ctgcaaaacc gcaggatgtg cagcaattac gcgctcgact tcttccggct
    23761 gaataccctc tccgccactg aagaacagat tgtctaaacg tccgacaatg gtcagcttgc
    23821 cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa
    23881 ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc cacacttcat
    23941 tattaacgat tttcacttcc cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg
    24001 cttctttcgc acacaccgtg gaggcaaact cggtcagacc atagccgcaa aagcaacgaa
    24061 tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg ccaagtaaca
    24121 ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt gttggcacca
    24181 gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca cgcaccgtca
    24241 tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg tgaaacagcg
    24301 gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac acaccttgcg
    24361 cactggcaag atgggcctga taagtatgga cagcggcttt cggcaaaccg gtagaacccg
    24421 aggtcaacgt cattgagcac agacgcgtcg gctgccacgt agcggcatgt gcgccttcaa
    24481 cccgctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg
    24541 caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc agctgagggt
    24601 tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg
    24661 gcgtattcca cgcccgcaac atcacgccgc tgccctcaac cacaccctgc gccgcaaagc
    24721 ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa
    24781 gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc cagtcagaga
    24841 agatcataac aactgctcca gtacatcagc gtccactaac ggcagtgggc tccccggcca
    24901 gcgacgaacc tgctgcgcct gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg
    24961 cgttaaccag gcagcaatcc gcgccagttg cgttagacct aagctcgact caatggaaga
    25021 actgatcacc gccgtcagcc ccagcgcatg cgccgcctgt acctgctcgc gtactttttc
    25081 cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt cagccacaaa
    25141 ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc
    25201 aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga tgcggtggcg
    25261 ataatccggg ttaacatatt tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt
    25321 tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattaa ccaccatgcc
    25381 gtcgcgcacg gcttcgtaca atcccacttt gaccttcgcc actttctcgc ctggcatatc
    25441 agcaagtttg aggatcagat cgtcaggatc gccattacac agtggtgccg cacggtaatt
    25501 ggccgcctgc gggagcgtat ctgccagctc cgccaatgca cagcttacgc caaaggcaac
    25561 ggaaggcatc tgcggtagct cgccatcgcc tgccagccag ttatttaccc aagcaagcag
    25621 cacaatttgc gcctcttccc aggtttcctg actgaagccc ggcagtgggg agatctcccc
    25681 ccacccttcg cgctcgcctt cacgcaggca aacatacagc ccgtcgcggg tttttaaccg
    25741 cctgtcgcgc agaaccatcc ccgcgtccat ggggatctgc cagcggtata cctgcgcgct
    25801 acgcattacg gattccgttt aaatttgctg aaatcaggct gacgtttctg gttgaaagcg
    25861 ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt gcccgccagc
    25921 tcctgcagac ccgcctgacc gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc
    25981 gccatcgggc tgttttgcag catttcgcgg caccaacgga cggtttcttt ttccagatcc
    26041 gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc atcgtactga
    26101 cggcacagga accagatttc acgcgctttt ttctgtccga cgatgcgagc catgtaggaa
    26161 gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa gatggcatta
    26221 tctgccgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc gatggagtag
    26281 ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg gaagtccagc
    26341 acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc acgcactttc
    26401 tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat cacgccgacg
    26461 ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt cagaggacgg
    26521 aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc ggtggatttt
    26581 tcataacgaa tgtcctcaaa accttcggag cagtcgtgcc attcaaccgg tgcgtaaagc
    26641 attgcttcat caggataaat catattgtgt cctttagtca gaaacgcaag atctgcgcca
    26701 gacttgcgat aacgccagcg ggattttccc gatgcgcgtt atgtccggcg cgaggaatga
    26761 catggcagtc ggcagccagt tccgccgcca aggcgcggaa tttgctgtca cgttcaccac
    26821 ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca ggctggacgg
    26881 cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgtta ctgcgcagcg
    26941 ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt tgataccagt
    27001 cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac
    27061 gctgacgttc cgcgcgttgt tcagcatttt gcaacccagg atgcccgcct tcgacaacaa
    27121 ccccacaaag ccccggcatc ccctggcaag ccgccatcat cgccacccgt cccccaagcg
    27181 agtaccccac caaccagaag ttaaggatgt tgtaactaac cagggtttta cgcagtaagt
    27241 cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccgtga cctgggagat
    27301 aaacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat
    27361 cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggtttgccg tgttttgcct
    27421 gcgcgtgcag gatcataaat ggcttacctg cgccagaagt tgctggagcg tttgcgcacc
    27481 atcggtgtcg ttaaccacca tttcaattac cgtggtggtt ggcgtgcgcc aggcatcggc
    27541 aagtgccgtt tcaagttcct gccagttttg cggacgatga tatttcagct cgaacatcgc
    27601 ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcgc gctcgctttt
    27661 cggcgttggc agcagcgaga aaatttgccc gccgttgttg ttcaccacaa ttaatatcag
    27721 cggcgcggaa acctgacgca ataacgccag cgcgttgaga tcgtaaagtg cggagagatc
    27781 gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgccggcgg cagtcgagag
    27841 cagcccgtcg ataccgctgg ccccacggtt gctgtacacc gggtaacctg ccggaagttg
    27901 cgaaagcgca tcaatcagac gtaccaccag gctgttaccg acaaacaatt gcccctgttc
    27961 aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat cgcgacgggc
    28021 aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc agggctggcg
    28081 tttttctgcc ggatgcagct ccagccagtc ggcaatattg gcaattaagc ggcgtccacg
    28141 atggtgcgcc ggatcaagcc gcccttcgat gtcatccaca atccagtact cttcgggttc
    28201 acagcttgcc tgccattgca ggagccgttt gcccgtcagg ctgcttccca gttgcaccac
    28261 aatttgcgcc tgttgcagtt cgctggtcgc tttggcattg cctaaccaga gatcggcaca
    28321 cggcagcggc tgtccggttt gcgacagcac gtcgccaatc agcggccagc caagagtttg
    28381 cgcccacagt gcaacttttt tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc
    28441 gcgtttttgt cgccagaaga accagtcgcg ctgtttttca ctttccagac gaggcgcttc
    28501 acgcagccac ggtttgtcgt cctgccacca gtcgcccagc cgctgttgcc agctaagccc
    28561 ggtgtcgtcc atttcgccat acagcggttc ggcaaacggg cagttgatat ggactccacc
    28621 agaatgaagc gtaccgagag catggtcgat ggtagaaacc agccaacgtg cggggatatc
    28681 ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga gaggcgaaca ttcccggctg
    28741 acgaatcgcc tgattcgcgc cgcagtcaat cagctccggc gggcgatcgg cggttaacag
    28801 gatcagtttt tctccggtta acccagcttc aatcagtgcc ggatagagat ttgctaccgc
    28861 cgtaccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca gccccagcgc
    28921 cagatgcccc aacccacgct catcgaaatg ggtgtggtga atgaatgcgg aattctccgc
    28981 cgccgctaac gttaacggtg tagaacgcga gccaggggcg atacagatgt gtctgacgcc
    29041 gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg cgcttactga
    29101 catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa aatagattat
    29161 tgatatgaat cggtaatgat gcgactcatt actattccat ctgtaataaa gtacgcagcc
    29221 ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca cggacaatgc
    29281 ccgcgccagc atataatcgc acgacattgc cgctaatttt tgctgagcgc agggaaacgc
    29341 agaattcgct ttgctgtaat gagagatagc ccgcagaacc ggcgtaccat tctcgggtga
    29401 acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg
    29461 ccgttggctg caactgatgt aaacagatca cattatccgc tttgttgagt gaagtccaga
    29521 tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt aaaacatcca
    29581 gcgtctgggt atcggcctgt aaccgttggc agatatcttc caccaccagc atattctcgc
    29641 gctggttttt atcatccgcc atcagccact cacctaactg ctgcgcctgc ttatcatcag
    29701 gatgatttgc taccgttccc gccagcgctt cagtacgcag cgctttgtca cgccgccgcc
    29761 ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat
    29821 ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgcg ttgaccggac
    29881 ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt gtcgagctca ccttcggcga
    29941 tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc cagtgttgtt
    30001 ctcgcgtggt ggttaaatgt aacccaggca agggcttgat actcaccagt gtggcgataa
    30061 attctcttgc cttaatcgca tcgtgctgaa gggagctttc gctgaatagc gtcagccgca
    30121 gcgtggcttt accgccacag cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg
    30181 acgggtcaaa cgcattcagc ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa
    30241 gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg acggcagctt
    30301 cttcatcacc attacgttgt tgccagtaga attgcgggta tgtttgctga ctggccagcc
    30361 agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc cgaatgccgg
    30421 gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg
    30481 attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact acaaaaaaaa
    30541 tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct agtaccagcc
    30601 caacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag tcatcagcac
    30661 gataaactgc ctgcttcgcc cgatagtaat aactgtctga agcctggcta acccgttttt
    30721 tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc tgatcggcgg
    30781 gatcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc aggtcgtcat
    30841 cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt aaaacctgtc
    30901 actaactata gacaagggtt gttgattacg cctgaattac ctcgcgtgcc atcccaatgt
    30961 gtgggatacc atcttcttca tagacctccg tcacagggat aaaaccgaaa ctctggtaga
    31021 agttttgcag atgcgcctgc gccccgaggt aaacaggctt atcaggccag tgatgcgtgc
    31081 aggtgtcgag ggttttactc atcagttgct gccccacttt ttcgccacgc aacgcttcac
    31141 tgacaatcac ccgaccaata acgaccggct caagatcatc atcacttttc agaatcctcg
    31201 catatgccac cagttcatca tttttccaac cgagaatatg gcgattatcc cccgtcaaat
    31261 catcaccatc gatatcctga taagggcaat tctgctcgac aacaaacacc gcgcaacgca
    31321 gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga tcttaccatt
    31381 caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat tttttgaaac
    31441 gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca acccgcacgc
    31501 gcaatgtcac ggcaatattg cttaacctgc aacatccgac ccagagcgga ctttggttgt
    31561 ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac cctgggaaac
    31621 tggacaagat ttttatcagt caccttcatg gcgatcatct ctttggttta cccggcttgc
    31681 tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt ccgcaaggta
    31741 tccgtgaatt tgtggaaacc gcgctgcgga ttagcggctc atggaccgat tatccgctgg
    31801 aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt
    31861 atccgatgga acacccactg gaatgttatg gctatcgtat tgaagaacat gataaaccgg
    31921 gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg
    31981 aattaaaagc gggcaaaaca atcacgctgg aagatggaag gcagattaac ggcgcagatt
    32041 acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg
    32101 atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg acgctggata
    32161 taaccatgga agccaaagcc aatagtcgcg gccatagctc tacacgccag gctgcgacac
    32221 tagcccgtga ggctggagtc ggcaagctaa tcattaccca cgtcagctcg cgctatgatg
    32281 acaaaggttg tcaacacctg ttacgtgaat gcaggtcaat tttcccggcg actgaactgg
    32341 cgaatgattt caccgtgttt aacgtttaac tc
//
LOCUS       DAJVXP010000035        31795 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVXP010000035 DAJVXP010000000
VERSION     DAJVXP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.035"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OQS10_001557"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OQS10_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS2292611.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OQS10_001558"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OQS10_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS2292612.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OQS10_001559"
     CDS             1894..2751
                     /locus_tag="OQS10_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2292613.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OQS10_001560"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OQS10_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OQS10_001561"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OQS10_001561"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS2292614.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OQS10_001562"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OQS10_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OQS10_001563"
     CDS             complement(7411..8658)
                     /locus_tag="OQS10_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS2292615.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OQS10_001564"
     CDS             complement(8680..9006)
                     /locus_tag="OQS10_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS2292616.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OQS10_001565"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OQS10_001565"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS2292617.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRATFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OQS10_001566"
     CDS             10395..11651
                     /locus_tag="OQS10_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS2292618.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OQS10_001567"
     CDS             11766..12092
                     /locus_tag="OQS10_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS2292619.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OQS10_001568"
     CDS             complement(12233..13471)
                     /locus_tag="OQS10_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS2292620.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OQS10_001569"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OQS10_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS2292621.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OQS10_001570"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OQS10_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OQS10_001571"
     tRNA            complement(17456..17531)
                     /locus_tag="OQS10_001571"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="OQS10_001572"
     tRNA            complement(17571..17646)
                     /locus_tag="OQS10_001572"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="OQS10_001573"
     CDS             17867..18226
                     /locus_tag="OQS10_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS2292622.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="OQS10_001574"
     CDS             18228..18620
                     /locus_tag="OQS10_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS2292623.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OQS10_001575"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OQS10_001575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS2292624.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="OQS10_001576"
     tRNA            20346..20421
                     /locus_tag="OQS10_001576"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20466..20541
                     /locus_tag="OQS10_001577"
     tRNA            20466..20541
                     /locus_tag="OQS10_001577"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20499..20501,aa:Val,seq:tac)
     gene            20588..20663
                     /locus_tag="OQS10_001578"
     tRNA            20588..20663
                     /locus_tag="OQS10_001578"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20621..20623,aa:Val,seq:tac)
     gene            20668..20743
                     /locus_tag="OQS10_001579"
     tRNA            20668..20743
                     /locus_tag="OQS10_001579"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20701..20703,aa:Lys,seq:ttt)
     gene            20774..20899
                     /locus_tag="OQS10_001580"
     ncRNA           20774..20899
                     /ncRNA_class="other"
                     /locus_tag="OQS10_001580"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21019..21360
                     /locus_tag="OQS10_001581"
     CDS             21019..21360
                     /locus_tag="OQS10_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS2292625.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21351..22277)
                     /locus_tag="OQS10_001582"
     CDS             complement(21351..22277)
                     /locus_tag="OQS10_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2292626.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22367..23365
                     /locus_tag="OQS10_001583"
     CDS             22367..23365
                     /locus_tag="OQS10_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS2292627.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23362..23580)
                     /locus_tag="OQS10_001584"
     CDS             complement(23362..23580)
                     /locus_tag="OQS10_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS2292628.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23582..25597)
                     /gene="ligA"
                     /locus_tag="OQS10_001585"
     CDS             complement(23582..25597)
                     /gene="ligA"
                     /locus_tag="OQS10_001585"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS2292629.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25668..26654)
                     /gene="zipA"
                     /locus_tag="OQS10_001586"
     CDS             complement(25668..26654)
                     /gene="zipA"
                     /locus_tag="OQS10_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS2292630.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26884..27645
                     /gene="cysZ"
                     /locus_tag="OQS10_001587"
     CDS             26884..27645
                     /gene="cysZ"
                     /locus_tag="OQS10_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS2292631.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27830..28801
                     /gene="cysK"
                     /locus_tag="OQS10_001588"
     CDS             27830..28801
                     /gene="cysK"
                     /locus_tag="OQS10_001588"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS2292632.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29185..29442
                     /gene="ptsH"
                     /locus_tag="OQS10_001589"
     CDS             29185..29442
                     /gene="ptsH"
                     /locus_tag="OQS10_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS2292633.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29487..31214
                     /gene="ptsI"
                     /locus_tag="OQS10_001590"
     CDS             29487..31214
                     /gene="ptsI"
                     /locus_tag="OQS10_001590"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS2292634.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31255..31764
                     /gene="crr"
                     /locus_tag="OQS10_001591"
     CDS             31255..31764
                     /gene="crr"
                     /locus_tag="OQS10_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS2292635.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7842 a         8383 c         7907 g         7663 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgtggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aactacttta tgtagtctcc gccgtgtagc aagaaattga
    20461 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20521 cgatcccgtc atcacccacc aatttctcgc cagctaaatt tcttgtaaaa atgtgaagta
    20581 ccgaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    20641 ttcgatcccg tcatcaccca ccacttcggg tcgttagctc agttggtaga gcagttgact
    20701 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct
    20761 aaaggcgctt ttttgctatc tgcgatactc aaagattcga acctgcagca ggtttgagtt
    20821 gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc gaagggcgag cgaagcgagt
    20881 catcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
    20941 atttgtaaga ttgatttttc ttctcaagtt ccttgttctt ctgccgatat tacctcgtac
    21001 agtgacgagg actgaacaat ggccactatc tcttcaactt caattccttc catacaaaca
    21061 cagagtagta acagggcatc acaaggtagc gatgttgctt caaaaatcgc gcggataagt
    21121 cagcaaatca tcaaactcac tcaacaaatt aaagagattg tagacactag cggcagtgct
    21181 gaagacaaac aaaaacaggc cgaattgatt caacagcaaa tcaccctact ggaaacacaa
    21241 ctggcgcagt tgcagagaca gcaggcagaa aaggcgcagg agaaagagca gcgtttgtct
    21301 ctgaatgtat cccttctaaa tcctgttgaa aatacgactc atatcgatat ctatatctga
    21361 tacaacggag ccccttcccg accagccatt agcgcattgc cctggtctcg taccacgtcc
    21421 cataacgctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg
    21481 cgttcaacaa ccggcgtaat gcgctttacc accagtgggc taccttcagg caatggtagc
    21541 gccagtgcgg gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc
    21601 ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca
    21661 atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc
    21721 tgccagggta cataatcttc cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa
    21781 ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg
    21841 caaaaatcga catctccctg gcgaatactt tccatcaccc actgctgcgg acgatcgtgc
    21901 aggacaaact gaatatctgg atagcggcga tggctttcag caatgcattg cggaataaga
    21961 tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata
    22021 cgcccggtat cgcgcaacgt gctgttaagt tcatccagca gtcgctcaag acgcaaagcc
    22081 aactgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caggcgaaca
    22141 ccagtatgat tttccagctc cttcacactg tgacttactg ccgactggct caagccgata
    22201 cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt
    22261 tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct
    22321 caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg
    22381 tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc
    22441 ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcta ttgccctgct
    22501 gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg
    22561 gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc tgggtgtact
    22621 gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt
    22681 gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa
    22741 cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg ctggggattt tcctttcacc
    22801 attgctagtt ggtctggtga tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg
    22861 taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat
    22921 tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct
    22981 gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg
    23041 ctggggatca ttgctgttta tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt
    23101 agttaacgtc tttatggcac gccgactggg cttcaataag gcagatgaaa ttactatcgt
    23161 cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc
    23221 cacatcggtg atcggtatga tggtgctgcc cctgatgatt ttccatcaga tccaattgat
    23281 ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcgcagca
    23341 ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag
    23401 cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa
    23461 ctcatctttg cgggcaaacc acagcagata ctcctccggc aggtcgatta agcgacgccc
    23521 tttgtatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat
    23581 ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct
    23641 gcgccttcgc cagtttagat cctgcagctt cacccgctat caccagatcg gttttcttcg
    23701 acacgctgcc cgcgactttc gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg
    23761 acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct
    23821 cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga
    23881 tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc
    23941 caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcact tccagcgtgc
    24001 cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat
    24061 agaggaagcg agcaaaggtg gtttctttcg ctttttccag cgcgttaacc acgttttgtg
    24121 ccgattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat
    24181 ctgccggagt gtggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc
    24241 cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgcac
    24301 cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag
    24361 aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag
    24421 aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat
    24481 cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca
    24541 ccagcacgcc agcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac
    24601 gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa
    24661 acgctaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgttccagtg
    24721 agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca
    24781 ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga
    24841 ccggcaaccc ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc
    24901 caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac
    24961 gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc
    25021 cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt
    25081 caccacgcac ttccagacgc gccgggatat tctctccgtg cagcttcagc ggaatggcgc
    25141 gaatagtacg cacattagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg
    25201 cactgactaa aacgccattt tcatacagaa tactgacggc aaggccatcc agcttcagct
    25261 cacagcacca ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag
    25321 caagaaagct ttcttcatca aaaacgttat ccagtgacag cattggtact tcatggcgta
    25381 tctggctgaa agccgccagc ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa
    25441 tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag
    25501 cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg cgaagcgtcg
    25561 ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac
    25621 ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg
    25681 tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg
    25741 cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc
    25801 atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa gatagtgaca
    25861 cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc
    25921 aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata
    25981 tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg
    26041 ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc
    26101 ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc
    26161 tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc
    26221 tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc
    26281 atcggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
    26341 atcggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga
    26401 cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc
    26461 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
    26521 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
    26581 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
    26641 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
    26701 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
    26761 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
    26821 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
    26881 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
    26941 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
    27001 attttgttga tggggggcgc attctggtgg ctctttacac agctcgatgt ctggatcccg
    27061 actctcatga gttacgttcc ggactggcta caatggctga gttatctgtt gtggcctctg
    27121 gctgtcatct ctgtgctgtt agtgtttggc tatttcttct ccacgattgc taactggatt
    27181 gccgctccgt ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca
    27241 ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
    27301 tggcaaaaat ttgcctggta tctgccgcgc gcaattgtat tactaattct ttacttcatc
    27361 cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
    27421 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
    27481 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt
    27541 acgatgatcc cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg
    27601 atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat
    27661 tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
    27721 gtcattattt cccttctgta tatagatatg ctaaatcctt acttccgcat attctctgag
    27781 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat
    27841 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg
    27901 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
    27961 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
    28021 actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
    28081 cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
    28141 gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat
    28201 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
    28261 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga tctgggaaga
    28321 taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg
    28381 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga
    28441 gccaaccgat tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggccc
    28501 gcataaaatt cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt
    28561 cgataaagtc attggcatca ccaatgaaga agccatttct accgcccgtc gtctaatgga
    28621 agaagaaggt attcttgcag gtatctcttc tggagcagct gttgccgcgg cgttgaaact
    28681 acaagaagat gaaagcttta ccaataagaa tattgtggtt attctaccat catcgggtga
    28741 gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta
    28801 atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
    28861 ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
    28921 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
    28981 tgatttggtt caattcttcc tttaacggca taatgtttaa tgacgtacga aacgtcagcg
    29041 gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac
    29101 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
    29161 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
    29221 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
    29281 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
    29341 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
    29401 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
    29461 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
    29521 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
    29581 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
    29641 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
    29701 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
    29761 tgattaaaga taaacacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
    29821 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
    29881 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca
    29941 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
    30001 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
    30061 tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg
    30121 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
    30181 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
    30241 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtat
    30301 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
    30361 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag
    30421 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc
    30481 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
    30541 acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg
    30601 atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga
    30661 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
    30721 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
    30781 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
    30841 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    30901 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    30961 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    31021 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    31081 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    31141 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    31201 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    31261 ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag
    31321 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    31381 gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg
    31441 cctgtagacg gtaccattgg taaaatcttt gaaaccaatc acgcgttctc tatcgaatct
    31501 gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    31561 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa
    31621 tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    31681 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc
    31741 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcg
//
LOCUS       DAJVXP010000036        30870 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVXP010000036 DAJVXP010000000
VERSION     DAJVXP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.036"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..948
                     /gene="tuf"
                     /locus_tag="OQS10_001592"
     CDS             <1..948
                     /gene="tuf"
                     /locus_tag="OQS10_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS2292636.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1178..1561
                     /gene="secE"
                     /locus_tag="OQS10_001593"
     CDS             1178..1561
                     /gene="secE"
                     /locus_tag="OQS10_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS2292637.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1563..2108
                     /gene="nusG"
                     /locus_tag="OQS10_001594"
     CDS             1563..2108
                     /gene="nusG"
                     /locus_tag="OQS10_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS2292638.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2267..2695
                     /gene="rplK"
                     /locus_tag="OQS10_001595"
     CDS             2267..2695
                     /gene="rplK"
                     /locus_tag="OQS10_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS2292639.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2699..3403
                     /gene="rplA"
                     /locus_tag="OQS10_001596"
     CDS             2699..3403
                     /gene="rplA"
                     /locus_tag="OQS10_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS2292640.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3816..4313
                     /gene="rplJ"
                     /locus_tag="OQS10_001597"
     CDS             3816..4313
                     /gene="rplJ"
                     /locus_tag="OQS10_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS2292641.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4380..4745
                     /gene="rplL"
                     /locus_tag="OQS10_001598"
     CDS             4380..4745
                     /gene="rplL"
                     /locus_tag="OQS10_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS2292642.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            5065..9093
                     /gene="rpoB"
                     /locus_tag="OQS10_001599"
     CDS             5065..9093
                     /gene="rpoB"
                     /locus_tag="OQS10_001599"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS2292643.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9170..13393
                     /gene="rpoC"
                     /locus_tag="OQS10_001600"
     CDS             9170..13393
                     /gene="rpoC"
                     /locus_tag="OQS10_001600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS2292644.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OQS10_001601"
     CDS             complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OQS10_001601"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS2292645.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OQS10_001602"
     CDS             complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OQS10_001602"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS2292646.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OQS10_001603"
     CDS             complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OQS10_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS2292647.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OQS10_001604"
     CDS             complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OQS10_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS2292648.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OQS10_001605"
     CDS             complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OQS10_001605"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS2292649.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OQS10_001606"
     CDS             complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OQS10_001606"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS2292650.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19080..19180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OQS10_001607"
     CDS             complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OQS10_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS2292651.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19804..20577
                     /gene="nudC"
                     /locus_tag="OQS10_001608"
     CDS             19804..20577
                     /gene="nudC"
                     /locus_tag="OQS10_001608"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS2292652.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20617..21681
                     /gene="hemE"
                     /locus_tag="OQS10_001609"
     CDS             20617..21681
                     /gene="hemE"
                     /locus_tag="OQS10_001609"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS2292653.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21691..22362
                     /gene="nfi"
                     /locus_tag="OQS10_001610"
     CDS             21691..22362
                     /gene="nfi"
                     /locus_tag="OQS10_001610"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS2292654.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22405..22995
                     /locus_tag="OQS10_001611"
     CDS             22405..22995
                     /locus_tag="OQS10_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS2292655.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23182..23454
                     /gene="hupA"
                     /locus_tag="OQS10_001612"
     CDS             23182..23454
                     /gene="hupA"
                     /locus_tag="OQS10_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS2292656.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23467..24162
                     /locus_tag="OQS10_001613"
     CDS             23467..24162
                     /locus_tag="OQS10_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS2292657.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OQS10_001614"
     CDS             complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OQS10_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS2292658.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24827..26203
                     /gene="zraS"
                     /locus_tag="OQS10_001615"
     CDS             24827..26203
                     /gene="zraS"
                     /locus_tag="OQS10_001615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS2292659.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            26200..27525
                     /gene="zraR"
                     /locus_tag="OQS10_001616"
     CDS             26200..27525
                     /gene="zraR"
                     /locus_tag="OQS10_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS2292660.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OQS10_001617"
     CDS             complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OQS10_001617"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS2292661.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OQS10_001618"
     CDS             complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OQS10_001618"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS2292662.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7226 a         8035 c         8518 g         7091 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      961 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
     1021 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
     1081 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
     1141 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
     1201 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
     1261 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
     1321 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1381 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1441 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1501 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1561 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1621 gccgcgtagc aacgtcgcta cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1681 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1741 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1801 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1861 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1921 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1981 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     2041 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     2101 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     2161 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     2221 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     2281 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     2341 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2401 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2461 ttctttcact ttcgttacca agactccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2521 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2581 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2641 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2701 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2761 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2821 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2881 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2941 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     3001 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     3061 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     3121 agtgggtact gtaacgccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     3181 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     3241 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     3301 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3361 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3421 tgattttgtc tacaatctta cccccacata taatgcttaa tgcagacgta tatccgagat
     3481 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3541 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3601 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3661 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3721 tgtttctgct caccgtaatt aagacgctct ctccgcttgg aggagtgaag tgagttccag
     3781 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3841 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3901 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3961 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     4021 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     4081 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     4141 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     4201 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     4261 gtaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     4321 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4381 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4441 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4501 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4561 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4621 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4681 aagacgacgc agaagcactg aaaaaatccc tggaagaagc tggcgctgaa gttgaagtta
     4741 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgactttttg
     4801 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4861 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4921 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4981 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     5041 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     5101 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     5161 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     5221 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     5281 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     5341 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5401 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5461 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5521 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5581 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5641 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5701 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5761 cgtgataaca agctgcagat ggagctggtg ccggaacgcc tgcgtggtga aactgcatct
     5821 ttcgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5881 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5941 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     6001 gcgaacatgg agctgagcct ggatctgttg gctaagctga gccagtctgg tcacaagcgt
     6061 attgaaacgc tgttcaccaa cgacctggat cacggcccgt atatctctga aaccttacgt
     6121 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     6181 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     6241 gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     6301 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6361 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6421 atccgttctg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6481 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggacatg
     6541 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6601 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6661 ctcggcccgg gtggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6721 actcattacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6781 aactctttgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6841 aaggtgaccg acggtgttgt aactgacgaa attcactacc tttctgctat cgaagaaggc
     6901 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6961 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac
     7021 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     7081 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     7141 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     7201 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     7261 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     7321 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7381 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7441 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7501 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7561 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7621 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7681 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7741 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7801 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7861 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7921 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtttgttc
     7981 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     8041 ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa
     8101 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     8161 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggtg
     8221 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     8281 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     8341 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8401 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8461 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8521 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8581 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8641 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8701 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8761 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8821 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8881 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8941 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     9001 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     9061 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     9121 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     9181 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     9241 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     9301 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9361 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9421 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9481 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9541 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9601 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9661 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9721 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9781 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9841 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9901 actgccgcca gatctgcgtc cactggttcc gctggatggt ggtcgtttcg cgacttctga
     9961 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
    10021 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
    10081 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
    10141 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
    10201 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
    10261 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
    10321 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10381 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10441 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10501 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10561 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10621 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10681 ggacgttgtt ctgggtctgt actatatgac ccgtgactgt gttaacgcca aaggcgaagg
    10741 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10801 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgcaaacg gtgaattagt
    10861 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10921 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10981 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    11041 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    11101 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    11161 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    11221 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    11281 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    11341 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11401 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11461 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11521 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11581 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11641 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11701 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11761 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11821 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11881 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11941 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    12001 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    12061 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    12121 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    12181 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    12241 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    12301 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12361 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12421 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12481 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12541 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12601 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12661 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12721 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12781 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12841 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12901 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12961 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    13021 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    13081 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    13141 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    13201 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    13261 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    13321 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13381 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13441 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13501 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13561 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13621 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13681 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    13741 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13801 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13861 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13921 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13981 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    14041 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    14101 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    14161 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    14221 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    14281 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    14341 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14401 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14461 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14521 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14581 cgctgcgccc gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc
    14641 atcatatcgt cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt
    14701 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag
    14761 gttttcatgc cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac
    14821 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14881 ccttcgccat gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt
    14941 cggcccccat ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    15001 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    15061 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    15121 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    15181 gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    15241 gaatctctaa ttttaaccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    15301 tggcttcttc cgctgttttc gccccggaag tatttggcag cagggtcaca cccgccgcga
    15361 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15421 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15481 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15541 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15601 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15661 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15721 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15781 caggctgcgc cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc
    15841 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac
    15901 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag
    15961 gcagcggtaa cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc
    16021 gaccgcgctg gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg
    16081 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt
    16141 taacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg
    16201 tgtcagtcgc tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag
    16261 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    16321 gacgccagcc cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16381 cagcacctgg ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcagaat
    16441 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16501 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16561 tgccgacacc cgttgctatc accgcaggcg cgcgcgccag actgataccg ccaatcgcca
    16621 cggtgggata atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg
    16681 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16741 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16801 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16861 catacgcctg atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16921 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca ttgcgccgat
    16981 ctttgatgcg tagctggagg gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    17041 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    17101 acatcacgct tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga
    17161 catatccgcc attcccactt caatggtttg cgcggcggcg taatcacgta cttcctggct
    17221 gattttcatc gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga
    17281 ctcttgcggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    17341 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17401 cgcgcctgga tgccctttcg ccaggtcagc ggcgtggacg gcaatcttat aggtgataag
    17461 cccctgctta acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca
    17521 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17581 atagcccggc gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17641 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc
    17701 ttcaatcatc acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg
    17761 cagctcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17821 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17881 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17941 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    18001 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    18061 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    18121 tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    18181 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    18241 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    18301 caggaaattg cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18361 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18421 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18481 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18541 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18601 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18661 gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc
    18721 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18781 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18841 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18901 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18961 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagtcaag
    19021 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    19081 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    19141 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    19201 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    19261 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    19321 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19381 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19441 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19501 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19561 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19621 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19681 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19741 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19801 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19861 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19921 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag
    19981 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    20041 caactggccg gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt
    20101 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    20161 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    20221 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    20281 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    20341 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20401 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20461 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20521 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20581 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20641 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20701 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20761 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20821 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20881 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20941 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    21001 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    21061 tggacgctgg cgacctacat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    21121 aaaatgatgt atgccgatcc gcaggcgctg cacgctctac tcgataaact ggcgaaaagc
    21181 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    21241 acctggggcg gtgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    21301 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21361 accaaaggcg gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgttgggc
    21421 ctcgactgga caacggatat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21481 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21541 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21601 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21661 tctgaacaat atcaccgcta aggagtgatt atggatctcg cgtcattacg cgctcaacaa
    21721 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21781 atcgccggag tcgatgtcgg gtttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21841 ctgctgaaat atccctcgct tgagctggtg gagtataaag ttgcccgcat cgccaccacc
    21901 atgccttaca ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg
    21961 gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    22021 cgccgtcttg gcgtcgctag ccattttggt ttgatggtgg atgtgccaac gatcggcgtg
    22081 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    22141 ccactgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    22201 ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    22261 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    22321 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    22381 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    22441 ctggaaagct ggcagcatgt cactttcatg gcttgcttat gcgaacgcat gtaccccaat
    22501 tacgccatgt tctgccagca aaccggtttt ggtgatgggc aaatttaccg tcgtattctc
    22561 gatctcatct gggaaacgct gaccgttaaa gacgcaaaag taaatttcga cagccaactg
    22621 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    22681 gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg
    22741 ctcgaacacg cggtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg
    22801 actcaggctg gtcgcgaaat gagcgatgaa gagcttaaag aaaacccagc tgtagagcaa
    22861 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22921 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22981 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    23041 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    23101 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    23161 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    23221 gcagaactgt ccaaaactca ggctaaagct gctctggagt ccactctggc tgcaattact
    23281 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    23341 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    23401 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    23461 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    23521 tcattgatgc tggctggcgc tctgcttctc actgcctgta gtcataactc ttcacttcct
    23581 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    23641 agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacg
    23701 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23761 aaaccgccgg tgaatattcg cgcgcgtttt gacgatcgcg gtgatctgag ctttatgcaa
    23821 cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23881 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23941 caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa
    24001 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    24061 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac
    24121 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac
    24181 ccatatgacc gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa
    24241 tacccgcttc agccatcgcg atatctcgtt tcacccgtaa ctcatctaac gactgacgca
    24301 aattctccat ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta
    24361 acagggcatt gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag
    24421 cgtaaaagtc attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg
    24481 cattttgctg ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca
    24541 ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc
    24601 cagtggttgt cttacttcgg gtattgcatc ttccgtgcca acgatgaaac gctgatatga
    24661 cgggtaatct ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt agcgagtcat
    24721 ttttactcat tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat
    24781 ggcatgattt ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca
    24841 acgttctaaa gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg
    24901 gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgccgc
    24961 cagacaaacg ctgctggaaa aaggcagtgt acttattcgc gctcttgaat ccggctcgcg
    25021 cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc
    25081 cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca
    25141 tagcaactcc ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca
    25201 tccgggagat gaagaagcgt ggcggcggat cgatagcgca gacggcgagc ctgttctgga
    25261 aatttatcgc cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca
    25321 gcagtatgcc gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag
    25381 tgccgaagat cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt
    25441 gctggcaagc gtattgtcat tcttctggta tcgccgctat ttgcgctcgc gccagcttct
    25501 acaagatgaa atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt
    25561 tgcccacgaa atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga
    25621 gcgcgcgcct gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga
    25681 ccgtttaaac cgcgtggtaa gcgagctgct ggaactggtt aagccaacgc atctggcttt
    25741 gcaggcggtg gatctcaaca cgctgattaa ccactcatta cagctggtaa gccaggatgc
    25801 gaacagccgg gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc
    25861 cgatccggac aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat
    25921 tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag
    25981 cgttaccgac agcggtaagg gaattgcggc agatcagctt gatgccatct tcactccgta
    26041 cttcaccact aaagccgaag gcaccggact ggggctggcg gtcgtgcata atattgttga
    26101 acaacacggt ggtacaattc aggtcgcaag ccaggaggga aaaggcgcaa cgttcaccct
    26161 ctggcttccg gtcactatta cgcgtaaggg cccacaagga tgacgcacga taatatcgat
    26221 attctggtgg tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc
    26281 tggggctata acgtcgcgct ggcgaacagc gggcgacagg cgctggagca ggtgcgggaa
    26341 caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg cattgccacg
    26401 ctaaaagaga tcaaagcgtt aaatccggcg attccggtgc tgattatgac cgcatactcc
    26461 agcgtcgaga cggcggtaga agcgttgaaa accggggcgc tggattatct catcaagccg
    26521 ctggatttcg ataacctgca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt
    26581 gacgctgaaa cgcctgcggt gtctgccagc cagttcggta tggtcggtaa aagcccggcg
    26641 atgcaacacc tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc
    26701 cacggcgatt ccggcaccgg taaagagctg gtcgccaggg cgattcacgc cagtagcgca
    26761 cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa
    26821 tctgaattgt tcggtcacga aaaaggggcg ttcactggag ccgatagacg ccgggaaggg
    26881 cgctttgttg aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg
    26941 atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc
    27001 aaccagacta tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca
    27061 gaggtgaatg ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt
    27121 gaagtaccat cgctgcggca acggcgggaa gatatccctc tgctggctgt ccattttctt
    27181 cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat
    27241 ttgctgatcc actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg
    27301 gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc
    27361 ggtacgccga tcccactggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa
    27421 gaagtgattc tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag
    27481 ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg
    27541 atagcgcgcc agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt
    27601 aaggcataag cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt
    27661 acgcgcccgc cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac
    27721 actttgccgc ctgccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa
    27781 tcgcccggat atccacccgc agccatcacc acgccgagag aagcacgctc atcccactct
    27841 gacgttttct cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac
    27901 ttcatgcgca gcataatcgg ctgggtttct ggatcgccaa agcggcagtt aaattcgata
    27961 accttcggat tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg
    28021 ttgccttccg ccgccatgcc tttcacggtt ggccagatga tacgttccat ggtgcgctga
    28081 tgaacttcat cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt
    28141 ccggtatctt tatcgcctac gcgtttgtga tcctggctgg tggccatcgg cagcacatgc
    28201 tcgccgtcta ccatcacgat aaagctcgct tcttcgccgt cgaggaactc ttcgataacg
    28261 atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcg
    28321 gcttcttcca gcgtcatcgc cacgataacg cctttcccgg ctgccagacc gtccgcttta
    28381 atgacgatcg gcgcaccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag
    28441 ttctggtatt ccgctgtagg gatcttatgg cgggccagga aatctttggt aaacgctttc
    28501 gaaccttcca gttgcgcggc acctgcggtt gggccgaaga ttttcatccc ggcggcgcgg
    28561 aaggtatcga ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc
    28621 ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt
    28681 tgcagcgcgg gttccagcgc agtgcctgca ttacccggag caacaaaaac agtttcaacc
    28741 agcggcgact gcgccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact
    28801 aatactttca tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg
    28861 caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct
    28921 ggattacgca ggtcacgcct gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga
    28981 acgcgtcaga agccatcgag gaacctttca cttccaagcc ttcatcggca gctttaatac
    29041 cggcgatttt cgcggagtac acgcggctca tctggcccgc gccaatcccg atggtcatat
    29101 tgtttttggc atagacgata gcgttggatt tcacgaactt cgccactttc cagcagaaca
    29161 gcgcatcacg cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg
    29221 caccgaccat gcccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat
    29281 caagacccgg aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg
    29341 cggcggtgat tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact
    29401 gacgagaaat gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc
    29461 cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat
    29521 tgccgatagc cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga
    29581 actctttcac gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt
    29641 taccctgaac ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga
    29701 aggcagcctg ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca
    29761 ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa
    29821 ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt
    29881 tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt
    29941 taataatggc gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag
    30001 cggcggagcg caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg
    30061 agcagccttc acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga
    30121 taggctggat ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc
    30181 caccatgtac tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg
    30241 tgtaatcgga aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc
    30301 ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg
    30361 ctttgtcaga aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc
    30421 ccctggattt gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac
    30481 gcccctaacg gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc
    30541 tcgcgaattt ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa
    30601 tacagaaatt acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt
    30661 tttgctgggg aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga
    30721 gaaatcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
    30781 cggttcggtt gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta
    30841 atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJVXP010000037        30004 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVXP010000037 DAJVXP010000000
VERSION     DAJVXP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.037"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            40..582
                     /locus_tag="OQS10_001619"
     CDS             40..582
                     /locus_tag="OQS10_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292663.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            665..1366
                     /locus_tag="OQS10_001620"
     CDS             665..1366
                     /locus_tag="OQS10_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2292664.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1391..3990
                     /locus_tag="OQS10_001621"
                     /pseudo
     CDS             1391..3990
                     /locus_tag="OQS10_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3981..5051
                     /locus_tag="OQS10_001622"
     CDS             3981..5051
                     /locus_tag="OQS10_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292665.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5063..5605
                     /locus_tag="OQS10_001623"
     CDS             5063..5605
                     /locus_tag="OQS10_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292666.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5613..6128
                     /locus_tag="OQS10_001624"
     CDS             5613..6128
                     /locus_tag="OQS10_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2292667.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6094..6831
                     /locus_tag="OQS10_001625"
     CDS             6094..6831
                     /locus_tag="OQS10_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS2292668.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6942..7952
                     /gene="pyrD"
                     /locus_tag="OQS10_001626"
     CDS             6942..7952
                     /gene="pyrD"
                     /locus_tag="OQS10_001626"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS2292669.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8126..8668
                     /gene="zapC"
                     /locus_tag="OQS10_001627"
     CDS             8126..8668
                     /gene="zapC"
                     /locus_tag="OQS10_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS2292670.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8665..9774)
                     /gene="ycbX"
                     /locus_tag="OQS10_001628"
     CDS             complement(8665..9774)
                     /gene="ycbX"
                     /locus_tag="OQS10_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS2292671.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10018..12126
                     /gene="rlmKL"
                     /locus_tag="OQS10_001629"
     CDS             10018..12126
                     /gene="rlmKL"
                     /locus_tag="OQS10_001629"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS2292672.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12138..14045
                     /locus_tag="OQS10_001630"
     CDS             12138..14045
                     /locus_tag="OQS10_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2292673.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14175..15428
                     /gene="pqiA"
                     /locus_tag="OQS10_001631"
     CDS             14175..15428
                     /gene="pqiA"
                     /locus_tag="OQS10_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS2292674.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15433..17073
                     /gene="pqiB"
                     /locus_tag="OQS10_001632"
     CDS             15433..17073
                     /gene="pqiB"
                     /locus_tag="OQS10_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS2292675.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17070..17633
                     /gene="pqiC"
                     /locus_tag="OQS10_001633"
     CDS             17070..17633
                     /gene="pqiC"
                     /locus_tag="OQS10_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS2292676.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17889..18056
                     /gene="rmf"
                     /locus_tag="OQS10_001634"
     CDS             17889..18056
                     /gene="rmf"
                     /locus_tag="OQS10_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS2292677.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18126..18644)
                     /gene="fabA"
                     /locus_tag="OQS10_001635"
     CDS             complement(18126..18644)
                     /gene="fabA"
                     /locus_tag="OQS10_001635"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS2292678.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18713..20473)
                     /locus_tag="OQS10_001636"
     CDS             complement(18713..20473)
                     /locus_tag="OQS10_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS2292679.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20659..21111
                     /gene="matP"
                     /locus_tag="OQS10_001637"
     CDS             20659..21111
                     /gene="matP"
                     /locus_tag="OQS10_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS2292680.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21187..22227)
                     /gene="ompA"
                     /locus_tag="OQS10_001638"
     CDS             complement(21187..22227)
                     /gene="ompA"
                     /locus_tag="OQS10_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS2292681.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22582..23091)
                     /gene="sulA"
                     /locus_tag="OQS10_001639"
     CDS             complement(22582..23091)
                     /gene="sulA"
                     /locus_tag="OQS10_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS2292682.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23310..23939
                     /gene="sxy"
                     /locus_tag="OQS10_001640"
     CDS             23310..23939
                     /gene="sxy"
                     /locus_tag="OQS10_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS2292683.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23902..26064)
                     /gene="yccS"
                     /locus_tag="OQS10_001641"
     CDS             complement(23902..26064)
                     /gene="yccS"
                     /locus_tag="OQS10_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS2292684.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26074..26520)
                     /locus_tag="OQS10_001642"
     CDS             complement(26074..26520)
                     /locus_tag="OQS10_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS2292685.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26643..28697
                     /gene="helD"
                     /locus_tag="OQS10_001643"
     CDS             26643..28697
                     /gene="helD"
                     /locus_tag="OQS10_001643"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS2292686.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28729..29187)
                     /gene="mgsA"
                     /locus_tag="OQS10_001644"
     CDS             complement(28729..29187)
                     /gene="mgsA"
                     /locus_tag="OQS10_001644"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS2292687.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29283..29945)
                     /locus_tag="OQS10_001645"
     CDS             complement(29283..29945)
                     /locus_tag="OQS10_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS2292688.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7734 a         7084 c         7880 g         7306 t
ORIGIN      
        1 aggcattatc atatttacga agagatcagg atgatgacga tgaaaaaaag tgtattgacg
       61 gcgtttataa ctgtggtatg tgcaacgtcc agcgttatgg cagctgatga taatgctatc
      121 acggatggca aagtaacatt taatggtaaa gttattgctc cagcttgtac cctggtagct
      181 gcgacgaaag attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat
      241 ggagcagtct ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact
      301 gtaactaaaa atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca
      361 tttgccaacc aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca
      421 gatggttcta catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattct
      481 gctgagcaaa cagtgacctt taaagttgat tatatcgcta cggggaaagc gacttctggt
      541 aatgtgaatg cggtaacaaa tttccatatt aactattatt aatagaactc attaattgtt
      601 ttattaatta gtacccctct agtgttctgg aggggatatt catatttttt aagagtgact
      661 atttatgaaa acttgcataa caaagggaat tgtgaccgta agtttaacgg caatattact
      721 cagctgctca tccgcatggg cggcaggtaa aggcgggatt ggacttgcag caacacgtct
      781 tgtttattca gaaggtgaag agcaaatttc actgggtgta cgtaacacca gtccggatgt
      841 tccttatctt attcagtcat gggtgatgac cccagataat aaaaaatcag cagattttat
      901 tattacacca ccgctatttg tgctgaatcc ggctaatgag aatctgttac gcattatgta
      961 cattggtgcg ccgttggcga aagacagaga aacccttttc ttcactagcg tacgggcagt
     1021 cccttcaaca acgaagcggg aagagggaaa taccctgaag attgccacac aaagcgtcat
     1081 caaacttttc tggcgaccaa aaggtttagc gtatccctta ggcgaggctc cggcgaaact
     1141 gcgttgcact tcgtcagctg acatggttac ggtcagtaac ccaacacctt atttcattac
     1201 cctgacagac ctgaaaatag ggggaaaggt agttaaaaat caaatgattt ccccctttga
     1261 taaataccaa ttctctctgc caaagggggc caaaaatagc agcgtaacgt atcgaaccat
     1321 caatgattac ggggcggaaa cgccacaact caactgtaaa tcgtaagccg tcttcagtta
     1381 agagagcgag atgtatagaa ctcatcgaca acacagcctg ttaagctctg gtggagtgcc
     1441 atcgtttatt ggtgggctgg tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac
     1501 ctggttcgat cctgcttttt tcaaagatga tccctcaatg gtggccgatt tgtctcgttt
     1561 cgaaaaagga caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac
     1621 aattgtagat acgcgcaacg tcaattttgt tgagataacg ccagagaagg ggattgccgc
     1681 ctgtttgacg actgaaagcc tggatgcaat gggtgttaat actgatgcgt ttccggcttt
     1741 taaacaactg gacaaacaag cgtgtgtgcc attggcggag attattccgg atgccagcgt
     1801 aacgtttaat gtgaataaac tccgtctgga aatttcagta ccgcaaatcg ccatcaaaag
     1861 taatgcccgt ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact
     1921 gggatattcg tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga
     1981 cagctatttt ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa
     2041 ttcaacatgg agtcgcagta gtggccaaac cgcagaatgg aagaatctca gcagctattt
     2101 gcagcgggcg gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg
     2161 cgattttttc gatagtgtca gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct
     2221 gccagacagc ctgaaagggt ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca
     2281 gataacgatt aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt
     2341 tgaaattagt gatctctatt ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga
     2401 agcggacggc agcgtcaata gctacagcgt accgttttcc agcgtgccat tactccagcg
     2461 tcaggggcga atcaaatacg cggtgacact ggcgaaatac agaaccaata gtaatgaaca
     2521 gcaggagagc aaatttgcct aggccacgtt gcagtggggc ggaccgtggg gaacgacatg
     2581 gtatggtggt ggacaatatg ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa
     2641 ccttggcgat ttcggagcaa tttcgttcga tgcgacccag gcgaagagta cgctggcaga
     2701 ccaaagcgaa cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt
     2761 gggcactaat tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc
     2821 cgacaccatg tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac
     2881 gccgatgtgg tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa
     2941 tatctcccag caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta
     3001 ctggcatacc gatcaacagg atcggctatt acagtttggc tacaacacgc aaattaaaga
     3061 tctctcgctg gggggttcct ggaactacag taagtcccgt ggtcaacctg atgctgatca
     3121 ggtgtttgca ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag
     3181 ctataccagg aaaaaaatta cgcctggatg acctctaaca ccagcatcga taacgaaggg
     3241 cacattacac aaaacctggg tttaacggag acactactcg atgacggtaa cctgagctac
     3301 agcgtgcaac agggatataa cagcgagggg aaaacggcta atggtagcgc cagcatggac
     3361 tacaaagggg cgtttgcaga tgcccgagtg ggctacaact acagcgataa cggcagtcaa
     3421 caacaactga actacgctct ttcaggcagt ttagttgccc attcacaggg cattaccctg
     3481 gggcaatcgc tgggggaaac taacgtgctg attgcagcac caggcgcaga gaatactcgt
     3541 gtggcgaaca gcaccgggct gaaaactgac tggcgcggat ataccgttgt cccctatgcc
     3601 acttcttatc gggaaaatcg aatcgcactt gatgcggcat cgttaaaacg taacgtggat
     3661 cttgaaaatg cagtagtcaa cgtggttccc accaaagggg cgttggttct ggcggagttc
     3721 aatgcccatg cgggagcaag ggtattaatg aaaacatcaa agcagggtat accgctgcgt
     3781 tttggcgcga tagcgacgct ggacggcgta cagactaata gcggcattat tgatgatgat
     3841 ggctcgctct atatgtccgg tttgcccgct cagggagcga taactgtacg ctggggcgaa
     3901 gctcccgatc aaatttgtca tatcagttac cagcttaccg aacaacaaat taactctgcg
     3961 attacgcgaa tggatgccat atgcagataa tctttggaga aaaatgcgtg ttattactac
     4021 gactattttt tgccgccgtc ttaatgctat ggtgcgctca aactgctgct tatagcgggc
     4081 agtgtcatac cactcagggg aatccgtata ttggcgtcaa ttttggcgtt aaaaccctgg
     4141 aggaagaaga aaatacggct ggagtagtta aagacaaatt ttatcagtgg aacgaatcga
     4201 atgattatta tgtttcctgt gattgcgata aagacaatgt cagaagtggc cgatgggcat
     4261 tcgccgcgga ttcaccgtta gtctatttag gcgacaactg gtacaaaatt aatgactatc
     4321 ttgccgccaa agttttattg caggttaaag gcagttctcc tactgcggtt cctttcgaaa
     4381 acgtgggcac aggggcagat acacgatggc atatttgcga tcccggcggt caacgtttag
     4441 gtggccaggg ggctagcggt aatagcggta gcttttccct gaaaatattg cagccgttcg
     4501 ttggttcggt cgtcattcct cctatggcgc tggcgcgatt atttgaatgc tacaacatac
     4561 ccgcaggtga ttcctgcacg actacaggta caccggtttt agtgtattac ctgtctggta
     4621 cgatcaattc acttggctca tgttccgtca atgccggaga aacaatcgag gtcgatctgg
     4681 gcgacgcatt tgcggctaac tttcgtgttg tagggcataa gcctcttggg gccagaacgg
     4741 cagaacttgc aattccagtc aggtgtaaca cgggaaacgc ggggttagtt aacgtcaacc
     4801 tgagtctgac ggcaaccaca gaccccagct atccccaggc gattaagacg tcacgtcctg
     4861 gcgtgggcgt ggtggtgacc gatagccaga acaacattat ttcccctgct ggtggaacat
     4921 taccgctctc tattcctgat gatgcagaca gtatcgcgcg aatgaatgtc tatccagtca
     4981 gcacgacagg tgtaccacca gaaaccgggc gatttgaagc cacggcaacg gtgagaataa
     5041 attttgatta acgcgtgaac gtatgaagaa agaaacgata tttcagtgcg ttatcttgtt
     5101 ctttagcatt cttaacatcc atgtcgggat ggctgggcct gaacaagtta gtatgcatat
     5161 ttatgggaat gtggtcgatc agggctgtga tgtcgccacc aaaagtgcat tacaaaatat
     5221 tcatattggt gattttaata tcagtgattt tcaggccgcg aataccgtaa gcactgctgc
     5281 tgatttgaat atcgatatca ccggttgtgc cgctagtatt actggcgcgg acgtcctttt
     5341 tagcggcgag gctgacaccc ttacgccgac actgctcaaa ctaactgaca caggcggaag
     5401 cggtggtatg gcaacgggga ttgccgtgca aattcttgat gcgcaaagtc agcaagaaat
     5461 cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca cactcaaata
     5521 tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga cggcggttct
     5581 ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc tggattctgt
     5641 tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg gtcaacatta
     5701 gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc
     5761 agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg gagaaatctt
     5821 tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgacgtc accttttcag
     5881 gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg gatgcagcca
     5941 gcgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt aaccaggcga
     6001 gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg caattgcgac
     6061 ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt gtacttcatt
     6121 atgactaata cgtggaacag attggcgctc ttgattttcg ccgttttatc gctgctggtg
     6181 gcgggtgaat tgcgggcagg agtggtggtc ggcggaacgc gatttatctt tccggcagac
     6241 agagaatcga tatctatttt actgactaat accagtcagg aatcctggct tattaacagt
     6301 aaaatcaacc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc agcaccatta
     6361 ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt gcgcttgctg
     6421 agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc
     6481 agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt
     6541 aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta tcaacaatta
     6601 cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta ctacattaac
     6661 ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagttgt gccgcctaaa
     6721 agccagcgtc agacaagctg gtgtcaggca atcgcaccgt gtcatgtcgc ctggcgggcg
     6781 ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg aaacaggttc
     6841 ggaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga actcgcacat
     6901 aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac cccttcgttc
     6961 gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt cagcaattac
     7021 gccgtattac aggaacgccg tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg
     7081 ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt cttgataaag
     7141 acggggagtg cattgacgcg ttaggcgcga tgggatttgg atcgatcgag atcggtaccg
     7201 tcacgccacg tccacagcca ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg
     7261 aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc gtagagaacg
     7321 taaaaaaggc ccattatgac ggcgtcctgg gtattaacat cggcaaaaat aaagatacgc
     7381 cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat gcctatgcgg
     7441 gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg ctgcaatatg
     7501 gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga
     7561 tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct gaagaagaat
     7621 tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt gcaaccaata
     7681 ccacactcga tcgttctctt gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa
     7741 gtggtcgtcc gcttcagtta aaaagcaccg aaattattcg ccgcttgtca ctggaattaa
     7801 acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa
     7861 agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt aaaggtccgc
     7921 cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct ctttataaga
     7981 taatcagggc tttattttca gccctggttg ttttatattc atcgctgttg cttatttaga
     8041 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
     8101 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
     8161 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
     8221 acgttttgcg cgcaagatgt tgactccaga tgccttttct cccgcaggct tttgcgttga
     8281 cgacgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt tatctaaaga
     8341 acaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgctatctca aaccgcaaat
     8401 gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
     8461 ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg ttgaatctgg
     8521 cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
     8581 gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga
     8641 cagcttcagc ctcgaacagg cagtctaacg cgccaactta agtgcagtct tcggtacaca
     8701 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcatgacttc
     8761 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
     8821 acgaattccc tgattttcta attgctccag caacacctgt tggttatttc cacgaaatgc
     8881 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc
     8941 atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt ccacctcatc
     9001 gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     9061 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     9121 ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     9181 aaacaccaca tcaccaatgc gaatcacttt ccagcgatct tcttcccagg ctgacgcgcc
     9241 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     9301 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     9361 aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg
     9421 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg caattcgcgc
     9481 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     9541 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     9601 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     9661 gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat gtgtaagacc
     9721 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     9781 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     9841 gcaacaattt tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggccctggg
     9901 ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc
     9961 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
    10021 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
    10081 aacctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
    10141 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
    10201 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
    10261 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
    10321 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg
    10381 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
    10441 tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg tttacatctg
    10501 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgctatt
    10561 gtgatgcgaa ccggctggca gccaggaaca ccgctgctcg atccgatgtg tggttccggt
    10621 acgttgctga ttgaagcggc gatgctagcg accgatcgcg caccaggctt gcaccgtggg
    10681 cgttggggct ttagcggctg ggtgcagcat gatgaagcta tctggcagga agtgaaagcg
    10741 gaagcgcaaa ctcgtgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg
    10801 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
    10861 gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
    10921 ccgtacggca cagtgttgag caacccgcca tacggggaac gtctggacag cgaaccggcg
    10981 ctgattgcgc tgcatagcct gctgggtcgg atcatgaaaa accagtttgg tggctggaat
    11041 ctctctttgt ttagtgcctc gccggatctg ctaagctgct tgcagctgcg tgcagacaaa
    11101 cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca cgttgccgaa
    11161 agcactccag acagcaaacc ggcgatggta gcggaagact acgccaaccg cctgcgtaag
    11221 aacctcaaaa aattcgagaa gtgggctcgc caggaaggaa ttgaatgtta ccgcctgtat
    11281 gacgccgatc taccggaata taacgttgcc gttgaccgtt atgccgactg ggtggtggtg
    11341 caggagtatg cgccgccaaa aactattgat gctcacaaag cgcgtcagcg tctgttcgat
    11401 attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt gctgaaaacc
    11461 cgtgaacgcc agaagggcaa aaaccagtac cagaaactgg gcgagaaggg cgagtttctt
    11521 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct cgatactggt
    11581 ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa aggcaaagat
    11641 ttcctcaacc tgttctctta taccggtagc gccaccgtgc atgcgggatt aggcggcgcg
    11701 cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg
    11761 cgtctgaatg gcctgaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
    11821 ctgcgtgagg caaatgaaca gtttgatctg atctttatcg atccgccaac cttctctaac
    11881 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atatggcgct gatgaaagat
    11941 ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa acgtggcttc
    12001 cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
    12061 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
    12121 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
    12181 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
    12241 tgggtcgcaa cggcgcgggc aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
    12301 tggatgacgg tcgcattatt tacgagcaag atttgattgt agctcgtctg caacaggagc
    12361 cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt gaagaacaag
    12421 cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
    12481 aaaatctcaa cgaactggcg aaggttcagg aacagttgga tcaccacaac ctgtggcagc
    12541 tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaat gttgcgctgt
    12601 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagtaatc
    12661 cgcgcgtgct gttgcttgat gaaccgacaa accacctgga tattgaaacc atcgactggc
    12721 tggaagggtt ccttaaaacc ttcaatggga cgattatttt catctcccac gaccgttcgt
    12781 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg gtgacctatc
    12841 cagggaatta cgaccagtac ctgcttgaaa aagaagaagc cctgcgcgtg gaagaactac
    12901 aaaatgccga gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca
    12961 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgcgagc
    13021 gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
    13081 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg
    13141 tgaaagattt ttcggcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
    13201 ggtgcggcaa aaccacgctg ctgaaactga tgctcgatca gcttcaagcg gacagcgggc
    13261 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc gcggaactgg
    13321 atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg atggttaacg
    13381 gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg aaacgggcga
    13441 tgacgccggt acgtgcgctt tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt
    13501 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgtcgaaa
    13561 cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg ctggttagcc
    13621 acgatcgtca gtttgtcgat aacaccgtta cagaatgttg gatcttcgaa ggcggcggta
    13681 aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg tcagcaagag cagtatgtgg
    13741 cgctcaaaca gcctgcggtg aaaaaaaccg aagaagccgc cgcggcaaaa gcagaaactg
    13801 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
    13861 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcagatg gcggatgctt
    13921 ccttcttcag tcagccacat gagcagacac aaaaagtgct cgccgatatg gctgctgcag
    13981 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
    14041 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
    14101 aactgggttt atcaggcatg aaaaccaaca ctttgttagc catctgtaaa atagcgcatc
    14161 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
    14221 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt
    14281 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtccgacc gcctatgcgt
    14341 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
    14401 caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
    14461 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
    14521 taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg
    14581 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gctggtgtgc
    14641 tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc agctttctcc
    14701 cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt
    14761 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg
    14821 ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct gctgatgaac
    14881 ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc cagcagtgga
    14941 cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
    15001 tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg gtcattctgt
    15061 tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt attatggtgc
    15121 caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg catggcaagc
    15181 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
    15241 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
    15301 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
    15361 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
    15421 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
    15481 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
    15541 cattacagcc atcagggact ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa
    15601 ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
    15661 ctggctgatg atttgacgca cgttgaaatc aaagtgcggc tgaattctgg tatggaaaaa
    15721 ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg cgaagggatt
    15781 agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg cgcgaaaggc
    15841 agcaaaatgg ataaatacga tttgctggac tcgccaccgt tggccccgcc tgatgcgaaa
    15901 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatccggtg
    15961 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac gcaaaaacgc
    16021 aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
    16081 cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat gcgtgtggag
    16141 atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc ggaaggtctg
    16201 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
    16261 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
    16321 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
    16381 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
    16441 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
    16501 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
    16561 ctggtcactg gtgcactgta tgttgatctt gatttctatc caaatacgcc tgcaataacc
    16621 ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg cctggcgcaa
    16681 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
    16741 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
    16801 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt gccgacggat
    16861 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
    16921 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
    16981 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
    17041 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac
    17101 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
    17161 tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg gtagagcagg
    17221 tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
    17281 atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacaattg cgcaacaccc
    17341 tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
    17401 gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat gatggcaagg
    17461 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
    17521 gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagta ctggccagtg
    17581 tctggagtca ggaagccgct tctattgcgc aagagataaa gcgtctacct taattataaa
    17641 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaacgt
    17701 gcggaaaaag ttgttcctct ttacattttt tgtacaaccg acatacccgt gtagctcaca
    17761 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
    17821 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
    17881 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
    17941 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt
    18001 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    18061 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    18121 tataatcaga aggcagaagt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    18181 atcaaacgac catcaaccag cacttctcca tccgccaggc ccataatcag acgacggtta
    18241 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgacca
    18301 gtgaatttca cttcgccaac gcccagtgcg cggcctttac cttcgccgcc cagccagccg
    18361 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    18421 tcgccaataa agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    18481 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc
    18541 agcatattcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag
    18601 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    18661 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    18721 gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    18781 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    18841 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    18901 atttgccttc ttctaccgct ttcactagtt cactgtgaag acttaaatgg cgaacgttag
    18961 ctgtggggat aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga
    19021 agccttcgat tttctcattt aaaccaccga ccggctgggc gcgaccgaac tgatcgactg
    19081 aacctgtgat agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc
    19141 agagttcagc catcgaggca ctatctccat caacttcact gtatgactgc tcaaatgtca
    19201 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcttgca
    19261 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    19321 tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    19381 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    19441 cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca gcgaggaaac
    19501 cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    19561 catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    19621 gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc caggcatccg
    19681 ctcccggtgc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga caccactggg
    19741 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    19801 ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    19861 tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa
    19921 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    19981 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    20041 gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgctccg
    20101 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    20161 ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgaccgccc accagttggc
    20221 ctgcatcgct ttgtagcgta cgcgcggcat tggcaataag attcagatac tcagactctt
    20281 ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    20341 caaattgcag ccgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    20401 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    20461 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    20521 cggcactaca gcacacgtag aaagcaccga caatactcca ggcatgggcg ttaaagctca
    20581 caggatggag attcttttct tcactggcct aaaaagctga tattctgtaa agagttacac
    20641 ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa
    20701 tggaagtatc tggtgaagaa gcatcgcgaa ggggagttaa tcacccgtta catagaagcc
    20761 agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta
    20821 aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt
    20881 cgggcaagac gaaagcgcca ttttaatgcg gaacaccagc atacgcgcaa aaaatcgatc
    20941 gatctggaat ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg
    21001 ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat
    21061 aaaatgtctt ctctgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa
    21121 gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca gcggggtttt tctaccagac
    21181 gagaacttaa gcctgcggct gagttacaac gtctttgata cctttaactt cgatctctac
    21241 gcgacgatcc ggagccaggc agtcgatcag tgcagcacgc tgtttcacgt tgtcacaggt
    21301 gttgccagta accgggttgg attcgcccat accacgtgcg gagatcttgt ctgccgggat
    21361 acctttggag atcaggtaat caacaacaga ctgagcacgg cgctcggaca gaccctggtt
    21421 gtaagcgtca gaaccgatgc ggtcggtgta acccagaaca actacggaac cgtctttcgg
    21481 atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag
    21541 ggttgctttg ttgaagttga acagaacgtc agacttcaga gtgaagtgct tggtctgtac
    21601 ttccggtgcc ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg
    21661 gtaggaaacc cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc
    21721 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    21781 ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg tggtttttac cataaacgtt
    21841 ggatttagtg tctgcacgcc ataccatacc acccagacga gtgtagatgt ccaggtcgtc
    21901 agtgattggg taacccagtt tagcggtcag ttgaacgccc tgagctttgt atgcaccgtt
    21961 ttcaacgctg cctttgtacg gcatacgacc taaccagtcg taacccattt caaagccaac
    22021 atacgggtta acctggtaac caccaaaagc accagcgccc agttggtttt catgggtcgg
    22081 gccattgttg ttgatgaaac cagtgtcatg gtactgggac cagcccagtt tagcaccagt
    22141 gtaccaggtg ttatctttcg gagcggcctg cgctacggta gcgaaaccag ccagtgccac
    22201 tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg ttatcatcca aaatacgcca
    22261 tgaatatctc caacgagata acacggttaa atccttcacc gggggatctg ctcaattaac
    22321 tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt
    22381 tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtat
    22441 gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc gcttcagaca
    22501 agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc
    22561 caggattaat cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag
    22621 ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc
    22681 ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca
    22741 accgatcacc acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg
    22801 gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac
    22861 ccattcccga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc
    22921 gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata
    22981 gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact
    23041 tgctgcggat gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc
    23101 agcccctatg agttactgta tggatgtaca gtacatccag tgacaacaaa gatcaaccct
    23161 attttcggaa agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt
    23221 gccccttaag aataagttgc ctattttcgt agttaacgga tccgttaatg tgaatcattc
    23281 ttttatgtta tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata
    23341 aatcacaaga atacctggca acgttgggca caattgaata ccgatcattg tttggcagtt
    23401 acagcctgac cgttgacgac acggtgtttg cgatggtttc tgatggtgag ttgtatcttc
    23461 gggcttgtga gcaaagtgca cagtactgtg taaaacatcc gcctgtctgg ctgacatata
    23521 aaaagtgtgg ccgatccgtt accctcaatt actatcgggt tgatgaaagt ctatggcgaa
    23581 atcaactgaa gctggtgcgt ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa
    23641 gcacgcgcaa tacccgggaa agactgaaag atttgcccaa tatgtctttt catctggaag
    23701 cgattctcgg ggaggtgggg attaaggatg tacgggcgtt acgtatactt ggggcaaaaa
    23761 tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc
    23821 ttgaaggtgc cattatcggc attcatgaag ctgcgctccc ggtggcacgc cgccaggagc
    23881 ttgcagaatg ggctgactct cttacgccga aacaggagtt tcctgcggaa cttgagtaat
    23941 ctggcgttgc agacgaccaa tctcaggcag taatgcaatc aataatccaa cttgttgtac
    24001 gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc tgcatccgct gtttcaggct
    24061 agctaatgcc tcattgacgc gttcttcatc agcaggttga tgatgtaacg cgtcatcaac
    24121 atagcaaact gcgtcatcaa gaaacgccag aatttcagga ttagttaact gctcccggtg
    24181 agcaccgagg gctgagatat agctggtaaa cgtatggtta aggcacagca accgaaacgc
    24241 ggcttcgcga atttgcgggg taacgttcgg ctcgctggac atatttgata ccaccgacgc
    24301 cagctcagca tcacggttgt gtgcatcgcg gcgggcaata cgatacgcca gacggttatc
    24361 acgcccctga tggtattgct ccagtatggc atcgagatac cgacagttgg cctctgtggc
    24421 gcgttcgagc atgcgcggca gattgcgaaa ctgccagtca ggccagatgt agctcactgc
    24481 cgcccacgca atggcacaac caatcagcgt atcgattacg cgaggtaacg ctacttcaaa
    24541 accttcaccc agtaagttaa aacacagtag caccaaaagt gtgatgaaca tcgttgcatg
    24601 agcgtattgc acgttacgga aggcaaaaaa gagcacgccg gtaataacca gcagcaccag
    24661 ctgcccttcc agtgatggca caaaccacag cacaggaatg ccaatggcga tacctaccag
    24721 cgtaccaata atccttaact tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac
    24781 aaacaaactt gtcagcaaga tccaataccc gtgatgcatt ccggttatct gaatgatggc
    24841 gtagccgaag cacaatacca gcgacattct taccgcatga cggaagaggg cggattccgg
    24901 cgtgaagtga cggctaagac gcagccagat atcactcaac ccgtgcgggc tgtcatcagc
    24961 gagctcattt tcgtcattat tatggggtag tgcctgggcc tgttctgatt caattgttgc
    25021 cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat cccagtgttt tgagtaaatc
    25081 ggcgggtgcg ccgttatcgc gcatccgctc cagcgcagca tcaatatgcg taaaagcgcg
    25141 ctcaaaatgc ggatcatgtt gataaggctg acgcaacaaa atacagcgtg acagttgctg
    25201 gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg
    25261 aaaatgttca cgcaatgttt gatactgaat atgagaagag ctggcacgct cgtgaatatc
    25321 ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg
    25381 taagcgggtc agcagcgaga gtttcgtctg attcaatgtc gccatcagca gaccgttggc
    25441 gagagccaaa tcgtacagcg gtgcctggct ttcatcttca atatcaggat caaacatgcg
    25501 cgacttgagc tcaagataac gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag
    25561 cgggcggacc gggaacagca gatgaccaat aagtgtcagg acgttgtacc agacggcacc
    25621 ggccagcaga tacatcggct gctgatacca gtgctcatac agtgatgttc ccaacatagt
    25681 gtaaatggcg atcagcaatg caccgaaggc aattgttgca tagcgttgac ccagaccgcc
    25741 gagcaaaatg aagccgctgg tagagagcgt taagccaatc gcaaatagcc agggccaggg
    25801 aaacagcaat tctactgagg ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt
    25861 acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc gccgctgcca ccatccccag
    25921 cgttagcgga atcgtcagtt ttacatcacc cagccaccac ggaaacgctg tggttccaca
    25981 aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg ttccaggtat agcgtttgag
    26041 caaaggactt agcataaagg ccatattagt ggattattca aaacgacgac gtgcattggc
    26101 ttcacgcgca gcttgcgctg tttctaccga taccacgcga cgaccaaccg gccatagtgc
    26161 aatagcggca attttaaagt tcgcaatgcc gacaggaatg ccaatgattg aaatacattg
    26221 tgcgatgccc gttgcaatgt gcatcaggca taaccaccag ccaaagaaaa tcagccagaa
    26281 aatattcaat accgtaccgc cagtattcag cagcacattt ttgccagccg ggttcagttc
    26341 atcgacatga atagcttcat tgccataagg caccagagat agtttagtga tctcccagca
    26401 ggatcgtgtc agcggtaagg taaaaatcag cacaatactg accagagtcg ccaacagcca
    26461 gcccagagtg gtggcaaatc cgccaagcac aaagttcaga atgttcaaaa cggtacgcat
    26521 aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct
    26581 ggagcacgtt ttctctgacg gttacactga taagaaataa tttcgtgtgg atctacagag
    26641 tcatggaact gaaagcgaca acgcttggaa aacgtctggc acagcaccct tacgatcggg
    26701 cggtgatcct caatgccggg attaaagtct ccggcgatcg ccacgaatac cttattcctt
    26761 tcaatcaatt actggcgatt cactgtaagc gcggtctggt atggggcgag ctggaatttg
    26821 tactgccgga cgaaaaagtg gtgcgtctgc acggcaccga atggggcgag acgcagcgtt
    26881 tttaccatca tcttgatgct cactggcggc ggtggagtgg cgagatgagc gaaattgcgt
    26941 ctggtgtttt acgccagcaa ctggatttga ttgccacgcg cactggagaa aataaatggc
    27001 tgacgcgtga gcaaacctct ggcgttcagc aacaaatccg ccaggctttg tcggcgttgc
    27061 cgttgccggt taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc
    27121 aggcctggtt gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag
    27181 ccatgcttac cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg
    27241 cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa
    27301 gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt
    27361 cccctgagca aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc
    27421 ggattcgcga acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc
    27481 tgcatattat tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata
    27541 ccgctgcccg tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag
    27601 cgcaggcgaa gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag
    27661 gtaacttctg ggatgatgaa aaattacagc gtcgccttgc ctctcgcctc gatcgctggg
    27721 taagtctgat gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag
    27781 agattcgcga tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga
    27841 aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg
    27901 tgattctgga gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc
    27961 aggatatctc gtcgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga
    28021 cgacgttgtt cgctgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa
    28081 tgtcgctcac caccgctttc catgaaaact ttggtgaagg cgaacgttgt gatttagaca
    28141 cgacttaccg ttttaacagt cgtatcggta aggtggcaaa ccggtttatt cagcagaacc
    28201 caggccagct gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt
    28261 tattggatga gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg
    28321 aagagcgcat tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag
    28381 cggcaacacg ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc
    28441 aacaggcgga ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg
    28501 cggcgcggga gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg
    28561 ctgaagaacg gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac
    28621 tgtttaacaa agagaatccc tctccctttg tggaaatact gaaaaatctg gatgtgccgg
    28681 tggcgagaaa accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg
    28741 gtccgcgaga taacgctgat aatcggggat tagaatatcg accgcgtcgt tgaaatgcgg
    28801 cgactggatt atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt
    28861 cgccagacgc agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca
    28921 gaagaaaatc aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc
    28981 ccccattggg ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt
    29041 accggtagtg cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac
    29101 ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc
    29161 aggtaaagtg cgagtcgtca gttccataat gtacatccgt agttaacttt cctacagatt
    29221 actgtaagca cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa
    29281 tctcaggaag atggttgttt ttccgcccac tgcaggaaag tatttcgcgt ttgtgggtca
    29341 gccagtttaa accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata
    29401 gcatccagct cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat
    29461 tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca
    29521 atcgtttttg ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca
    29581 tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc
    29641 tccaggcgag gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccacc
    29701 agcggtgggg agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg
    29761 cgaaacacta actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga
    29821 gagctggaaa ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc
    29881 gtggtggcaa aaacggatac cggcaaacat aatgcaatta aggtggtcac gatgccggtt
    29941 ttcataggta gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta
    30001 atca
//
LOCUS       DAJVXP010000038        29548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVXP010000038 DAJVXP010000000
VERSION     DAJVXP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.038"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="OQS10_001646"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="OQS10_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCS2292689.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="OQS10_001647"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="OQS10_001647"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS2292690.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTQAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="OQS10_001648"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="OQS10_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS2292691.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="OQS10_001649"
     CDS             complement(3644..4030)
                     /locus_tag="OQS10_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS2292692.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OQS10_001650"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OQS10_001650"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS2292693.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OQS10_001651"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OQS10_001651"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS2292694.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OQS10_001652"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OQS10_001652"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS2292695.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="OQS10_001653"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="OQS10_001653"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS2292696.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="OQS10_001654"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="OQS10_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS2292697.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="OQS10_001655"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="OQS10_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS2292698.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="OQS10_001656"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="OQS10_001656"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS2292699.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="OQS10_001657"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="OQS10_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS2292700.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="OQS10_001658"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="OQS10_001658"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS2292701.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="OQS10_001659"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="OQS10_001659"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS2292702.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="OQS10_001660"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="OQS10_001660"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS2292703.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="OQS10_001661"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="OQS10_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS2292704.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="OQS10_001662"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="OQS10_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS2292705.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="OQS10_001663"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="OQS10_001663"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS2292706.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="OQS10_001664"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="OQS10_001664"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS2292707.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="OQS10_001665"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="OQS10_001665"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS2292708.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="OQS10_001666"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="OQS10_001666"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS2292709.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="OQS10_001667"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="OQS10_001667"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS2292710.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="OQS10_001668"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="OQS10_001668"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS2292711.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="OQS10_001669"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="OQS10_001669"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS2292712.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="OQS10_001670"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="OQS10_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS2292713.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7160 a         7119 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactca ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAJVXP010000039        28797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVXP010000039 DAJVXP010000000
VERSION     DAJVXP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.039"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQS10_001671"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQS10_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS2292714.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OQS10_001672"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OQS10_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS2292715.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OQS10_001673"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OQS10_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS2292716.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OQS10_001674"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OQS10_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS2292717.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OQS10_001675"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OQS10_001675"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS2292718.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OQS10_001676"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OQS10_001676"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS2292719.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OQS10_001677"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OQS10_001677"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS2292720.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OQS10_001678"
     CDS             complement(4604..5326)
                     /locus_tag="OQS10_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2292721.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OQS10_001679"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OQS10_001679"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS2292722.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OQS10_001680"
     CDS             6526..6744
                     /locus_tag="OQS10_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS2292723.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OQS10_001681"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OQS10_001681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS2292724.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OQS10_001682"
     CDS             complement(7478..7678)
                     /locus_tag="OQS10_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS2292725.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OQS10_001683"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OQS10_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS2292726.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQS10_001684"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQS10_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS2292727.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OQS10_001685"
     CDS             10175..12088
                     /locus_tag="OQS10_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2292728.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OQS10_001686"
     CDS             12088..13110
                     /locus_tag="OQS10_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2292729.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OQS10_001687"
     CDS             13104..13322
                     /locus_tag="OQS10_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS2292730.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OQS10_001688"
     CDS             13376..14245
                     /locus_tag="OQS10_001688"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS2292731.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OQS10_001689"
     CDS             complement(14300..14704)
                     /locus_tag="OQS10_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS2292732.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OQS10_001690"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OQS10_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS2292733.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OQS10_001691"
     CDS             15689..17779
                     /locus_tag="OQS10_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS2292734.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OQS10_001692"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OQS10_001692"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS2292735.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OQS10_001693"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OQS10_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS2292736.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OQS10_001694"
     CDS             complement(19747..20349)
                     /locus_tag="OQS10_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS2292737.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OQS10_001695"
     CDS             complement(20339..20506)
                     /locus_tag="OQS10_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS2292738.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OQS10_001696"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OQS10_001696"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS2292739.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OQS10_001697"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OQS10_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS2292740.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OQS10_001698"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OQS10_001698"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS2292741.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OQS10_001699"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OQS10_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS2292742.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OQS10_001700"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OQS10_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS2292743.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OQS10_001701"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OQS10_001701"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS2292744.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OQS10_001702"
     CDS             28334..28501
                     /locus_tag="OQS10_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS2292745.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAJVXP010000040        28678 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVXP010000040 DAJVXP010000000
VERSION     DAJVXP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.040"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1046)
                     /locus_tag="OQS10_001703"
     CDS             complement(<1..1046)
                     /locus_tag="OQS10_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS2292746.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OQS10_001704"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OQS10_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OQS10_001705"
     CDS             complement(2433..3281)
                     /locus_tag="OQS10_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS2292747.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OQS10_001706"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OQS10_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS2292748.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OQS10_001707"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OQS10_001707"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS2292749.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OQS10_001708"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OQS10_001708"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS2292750.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OQS10_001709"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OQS10_001709"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS2292751.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OQS10_001710"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OQS10_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS2292752.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OQS10_001711"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OQS10_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS2292753.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OQS10_001712"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OQS10_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS2292754.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OQS10_001713"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OQS10_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS2292755.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OQS10_001714"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OQS10_001714"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS2292756.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OQS10_001715"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OQS10_001715"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS2292757.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OQS10_001716"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OQS10_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OQS10_001717"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OQS10_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OQS10_001718"
     CDS             22669..23652
                     /locus_tag="OQS10_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS2292758.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OQS10_001719"
     CDS             23675..25186
                     /locus_tag="OQS10_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS2292759.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OQS10_001720"
     CDS             25211..26209
                     /locus_tag="OQS10_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2292760.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OQS10_001721"
     CDS             26242..27336
                     /locus_tag="OQS10_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS2292761.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OQS10_001722"
     CDS             27348..28220
                     /locus_tag="OQS10_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS2292762.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="OQS10_001723"
     CDS             complement(28268..>28678)
                     /locus_tag="OQS10_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS2292763.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6816 a         7093 c         7792 g         6977 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAJVXP010000041        28515 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVXP010000041 DAJVXP010000000
VERSION     DAJVXP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.041"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(301..507)
                     /locus_tag="OQS10_001724"
     CDS             complement(301..507)
                     /locus_tag="OQS10_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS2292764.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="OQS10_001725"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="OQS10_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS2292765.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="OQS10_001726"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="OQS10_001726"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS2292766.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="OQS10_001727"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="OQS10_001727"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS2292767.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="OQS10_001728"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="OQS10_001728"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS2292768.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="OQS10_001729"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="OQS10_001729"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS2292769.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="OQS10_001730"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="OQS10_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS2292770.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8860..8964
                     /locus_tag="OQS10_001731"
     CDS             8860..8964
                     /locus_tag="OQS10_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS2292771.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9275..11473
                     /gene="speF"
                     /locus_tag="OQS10_001732"
     CDS             9275..11473
                     /gene="speF"
                     /locus_tag="OQS10_001732"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS2292772.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11470..12789
                     /gene="potE"
                     /locus_tag="OQS10_001733"
     CDS             11470..12789
                     /gene="potE"
                     /locus_tag="OQS10_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS2292773.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OQS10_001734"
     CDS             complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OQS10_001734"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS2292774.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OQS10_001735"
     CDS             complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OQS10_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS2292775.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15241..16005
                     /gene="ybfF"
                     /locus_tag="OQS10_001736"
     CDS             15241..16005
                     /gene="ybfF"
                     /locus_tag="OQS10_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS2292776.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16145..16438
                     /gene="ybfE"
                     /locus_tag="OQS10_001737"
     CDS             16145..16438
                     /gene="ybfE"
                     /locus_tag="OQS10_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS2292777.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16578..17108
                     /gene="fldA"
                     /locus_tag="OQS10_001738"
     CDS             16578..17108
                     /gene="fldA"
                     /locus_tag="OQS10_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS2292778.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17318..17404
                     /gene="uof"
                     /locus_tag="OQS10_001739"
     CDS             17318..17404
                     /gene="uof"
                     /locus_tag="OQS10_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS2292779.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17397..17843
                     /gene="fur"
                     /locus_tag="OQS10_001740"
     CDS             17397..17843
                     /gene="fur"
                     /locus_tag="OQS10_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS2292780.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17928..18254)
                     /locus_tag="OQS10_001741"
     CDS             complement(17928..18254)
                     /locus_tag="OQS10_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292781.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OQS10_001742"
                     /pseudo
     CDS             complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OQS10_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OQS10_001743"
     CDS             complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OQS10_001743"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS2292782.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22212..24158)
                     /gene="nagE"
                     /locus_tag="OQS10_001744"
     CDS             complement(22212..24158)
                     /gene="nagE"
                     /locus_tag="OQS10_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS2292783.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24491..25291
                     /gene="nagB"
                     /locus_tag="OQS10_001745"
     CDS             24491..25291
                     /gene="nagB"
                     /locus_tag="OQS10_001745"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS2292784.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25351..26499
                     /gene="nagA"
                     /locus_tag="OQS10_001746"
     CDS             25351..26499
                     /gene="nagA"
                     /locus_tag="OQS10_001746"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS2292785.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26508..27728
                     /gene="nagC"
                     /locus_tag="OQS10_001747"
                     /gene_synonym="nagR"
     CDS             26508..27728
                     /gene="nagC"
                     /locus_tag="OQS10_001747"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS2292786.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27776..>28515
                     /gene="nagD"
                     /locus_tag="OQS10_001748"
     CDS             27776..>28515
                     /gene="nagD"
                     /locus_tag="OQS10_001748"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS2292787.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAE"
BASE COUNT         6684 a         7260 c         7480 g         7091 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
     8701 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
     8761 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
     8821 aactctacct tcaactgaca cagcaagagg taaaggtaaa tggaaaataa taaccgctta
     8881 atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa tagttttgac
     8941 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
     9001 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
     9061 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
     9121 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
     9181 tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga gaaattgagg
     9241 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
     9301 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
     9361 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
     9421 tgaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
     9481 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
     9541 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
     9601 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     9661 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     9721 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     9781 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     9841 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     9901 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     9961 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
    10021 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
    10081 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
    10141 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
    10201 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
    10261 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctggt
    10321 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
    10381 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
    10441 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
    10501 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
    10561 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
    10621 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
    10681 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
    10741 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
    10801 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
    10861 agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat
    10921 ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    10981 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11041 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11101 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11161 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgccga
    11221 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11281 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11341 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11401 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11461 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11521 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11581 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11641 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11701 tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt ctcactgctg
    11761 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11821 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    11881 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc
    11941 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12001 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12061 atgacgctgt gggcttttct cggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12121 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12181 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12241 acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg taaagtcatt
    12301 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12361 caggtgttta agtcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12421 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12481 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12541 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12601 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12661 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12721 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12781 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt tttattaaat
    12841 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    12901 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    12961 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    13021 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    13081 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13141 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13201 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    13261 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13321 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    13381 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13441 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13501 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13561 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcaat gccagcaggc
    13621 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcgaggtgca
    13681 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13741 cgccaattcg cttccagtat tcgataccgg aaccacccag cggatcaacg cccagcgtca
    13801 ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaactgct
    13861 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    13921 tcacgccttt caggccatcg gccatcagtg cgttggcacg atcttccacc actttagtga
    13981 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14041 ggttatggga gggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattatgaa
    14101 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14161 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14221 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14281 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14341 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14401 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14461 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14521 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14581 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14641 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    14701 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14761 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14821 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    14881 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    14941 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15001 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15061 atccagtaca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15121 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15181 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15241 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15301 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15361 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    15421 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    15481 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    15541 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15601 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    15661 actcgccagc aagcagcagc aataatgcac cagcatctta atgaagaagg ggtgattcag
    15721 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15781 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15841 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    15901 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    15961 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16021 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16081 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16141 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16201 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16261 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16321 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    16381 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16441 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16501 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16561 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16621 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16681 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    16741 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16801 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16861 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    16921 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    16981 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17041 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    17101 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17161 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    17221 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17281 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17341 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17401 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17461 tcctagaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17521 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17581 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17641 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    17701 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17761 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17821 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    17881 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    17941 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    18001 tgcgttgcac gcagatactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    18061 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    18121 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    18181 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    18241 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    18301 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18361 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18421 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18481 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18541 ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18601 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    18661 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18721 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    18781 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18841 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    18901 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    18961 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19021 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    19081 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19141 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19201 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    19261 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19321 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19381 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19441 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19501 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19561 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19621 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    19681 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19741 gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    19801 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    19861 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    19921 gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc cagcaagatg aaagcggtta
    19981 cattttattt aattatgttt tgttatttta aatttatttt atggataaat tgcgaagcgg
    20041 aattaattcg cgaagaaaag cagaaaaaac gccgctgaac gcggcgtttt ggattgtttg
    20101 catatttctt gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc
    20161 aatatattgc agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt
    20221 gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa
    20281 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20341 ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact
    20401 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20461 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20521 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20581 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20641 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
    20701 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20761 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc
    20821 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
    20881 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
    20941 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21001 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    21061 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21121 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21181 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21241 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21301 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21361 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21421 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21481 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21541 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21601 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21661 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21721 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21781 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21841 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21901 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21961 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22021 tgttagttag cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    22081 aagcggcaga gaagatctcc accgcttttg ccagtcccgc gtcattgttg gatgcgcgtc
    22141 ttatccaacc aggactgagc ttttgtaggc ctgataagac gcgtcaagcg tcgcatcagg
    22201 cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac aacattgccc
    22261 tgagctttaa tgatcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc
    22321 atcgaacggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg
    22381 ctcacctgcg cgccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg
    22441 tcgataccca tgtggacgac aatctccgcg cctttttcgg tttctaggca gaacgcgtgg
    22501 ttggtgttga agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc
    22561 ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc agaaacctga
    22621 tccagtgcca cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt
    22681 ggtacagcct gcggttttgc tacaggagcg gcagttgccg gagttgcttc agctgacgca
    22741 gctgctaccg ggccacgggc aacgactttc ttcatcgcat cgccaatgga ttctgctttc
    22801 gcgccaacaa tcacctgaat ggtctgtttg ttcagtttta ctacaccaga agcacccaga
    22861 cgtttacaca tcgcatcgtt gacgcgagct gagtcagcca cggtaaggcg cagacgggta
    22921 atacaggcgt caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt
    22981 gccagttgag tcagaccttc ttcagtattg ctgttagcct cttcagttac gatctcgtct
    23041 tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc
    23101 acgaagtaga tagcgaagaa gacaacaccc atcaccagca gcatccagac gttctggctg
    23161 gcggccggca ggttatacat caacgcgtag tcgatagccc ccgcagagaa agagaagcca
    23221 gcatggatac ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg
    23281 aggtacagca gcggagcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc
    23341 aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc
    23401 ggtgctgcga agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag
    23461 aagccggaca tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg
    23521 tggaaaaccg tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc
    23581 agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa
    23641 cccagcgcgc ctgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtactggc
    23701 ggccagacgt aaccaaaaat ggccgccagc accaggcaga aaaagccggt ggcaatcggc
    23761 acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg
    23821 ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt
    23881 tctgggttga tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc
    23941 gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat
    24001 gcgaggttat caaaaatcgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct
    24061 ggctgaccga atcgcagcaa cagtgccgcc accggcagca ccgcgatagg gagctgtaac
    24121 gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc
    24181 tatttggctc gtttaaagcc gtatttttat tttgctgcaa attgtactgc cgatgttctg
    24241 taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg tatcgcaaat
    24301 taaacgcgtg ccttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact
    24361 ggctaaagca gaaaacttat tttatcattc aaaaaatcag gtcggattga cgcctgtctg
    24421 cgcaaatcca ggttacgctt aaagatgcct aatccgccaa cggcttacat tttacttatt
    24481 gaggtgaata atgagactga tccccctaac taccgctgaa caggtcggca aatgggctgc
    24541 tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact gccgatcgtc cgtttgttct
    24601 gggcctgccg actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa
    24661 agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct
    24721 gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt
    24781 tgatattcca gcagaaaaca tcaaccttct caacggcaac gccccggata tcgacgccga
    24841 atgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg
    24901 tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac
    24961 tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga
    25021 tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacattgc tggatgccga
    25081 agaagtgatg attctggtgc tgggtagcca gaaagcactg gcactgcagg ctgcagttga
    25141 aggttgcgtg aaccacatgt ggaccatcag ctgtctgcaa ctgcatccaa aagcgatcat
    25201 ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga
    25261 attagaagca gaaaatatca aaggtctgta attgttatcc ctgccctggc tccttgctca
    25321 gggcaatatt ttttaaaatc ggggggcaga atgtatgcat taacccaggg ccggatcttt
    25381 accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc
    25441 gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt
    25501 ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac
    25561 accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca
    25621 ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc
    25681 gtgcgcgtta tgcgcgagta cctggcaaga catccgaatc aggcgttagg tctgcatctg
    25741 gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag
    25801 cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc
    25861 ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg
    25921 gtttctgccg gtcactccaa cgcgacgttg aaagaagcga aagccggttt ccgcgcgggg
    25981 attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc
    26041 ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg
    26101 catgttgatt acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg
    26161 gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa
    26221 acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc
    26281 ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa
    26341 gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg gcgttgagaa acgtctcggc
    26401 acactcgccg caggtaaagt agccaacctg acctcattca cacctgattt taaaatcacc
    26461 aagaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg
    26521 gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc
    26581 gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg
    26641 cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag
    26701 ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca
    26761 atttccacgc aatcggcgta cggcttggtc gtcacgacgc caccatcacc ctgtttgatc
    26821 tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcaaacgc
    26881 tggaacatgc cctgttgaat gccattgctc agtttattga tagctaccag cgcaaactgc
    26941 gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa
    27001 ttcattacat gccgcatatt caggtagaaa actgggggct ggtagaagct ctggaagaac
    27061 gttttaaagt gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact
    27121 acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg
    27181 gggccgggat tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa
    27241 ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc
    27301 tggaaactat cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg
    27361 gctaccagag ccgcgtgccg ctggacgact gcaccatcaa aactatctgc aaagccgcga
    27421 acaaaggcga tagtctggcg tcggaagtaa ttgagtatgt cggtcgtcat ctgggtaaaa
    27481 ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgcc ggtgaaatca
    27541 ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc caggcgctga
    27601 aggcgtttcg tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg
    27661 gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg
    27721 aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac
    27781 cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc
    27841 gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac
    27901 caactatcct tcgcagaccg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga
    27961 tgtgccggat agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcca
    28021 ggaaggcaag aaagcatatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc
    28081 cggtttcact attaccgatg tgaaccctga tttcgtgatt gttggcgaaa cgcgttccta
    28141 caactgggac atgatgcata aagcggccta tttcgtcgct aacggtgcac gctttatcgc
    28201 caccaacccg gacacccacg ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg
    28261 gattgagaaa atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat
    28321 ccgcgcagca ttaaacaaaa tgcaggcgca ttcggaagaa acggtgattg tcggcgataa
    28381 cttgcgcacc gacattctgg cgggcttcca ggcaggtctg gaaacgattc tggtgctttc
    28441 tggtgtttca tcgctcgacg atatcgacag tatgccgttc cgcccaagct ggatttaccc
    28501 gtcggtcgct gaaat
//
LOCUS       DAJVXP010000042        28266 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVXP010000042 DAJVXP010000000
VERSION     DAJVXP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.042"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..453)
                     /locus_tag="OQS10_001749"
     CDS             complement(<1..453)
                     /locus_tag="OQS10_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2292788.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFF"
     gene            complement(556..1911)
                     /gene="melA"
                     /locus_tag="OQS10_001750"
     CDS             complement(556..1911)
                     /gene="melA"
                     /locus_tag="OQS10_001750"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS2292789.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2194..3102
                     /gene="melR"
                     /locus_tag="OQS10_001751"
     CDS             2194..3102
                     /gene="melR"
                     /locus_tag="OQS10_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS2292790.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3301..5568
                     /gene="adiA"
                     /locus_tag="OQS10_001752"
     CDS             3301..5568
                     /gene="adiA"
                     /locus_tag="OQS10_001752"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS2292791.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            5893..6654
                     /gene="adiY"
                     /locus_tag="OQS10_001753"
     CDS             5893..6654
                     /gene="adiY"
                     /locus_tag="OQS10_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS2292792.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            6791..8128
                     /gene="adiC"
                     /locus_tag="OQS10_001754"
     CDS             6791..8128
                     /gene="adiC"
                     /locus_tag="OQS10_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS2292793.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8232..9875
                     /gene="eptA"
                     /locus_tag="OQS10_001755"
     CDS             8232..9875
                     /gene="eptA"
                     /locus_tag="OQS10_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS2292794.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            9872..10540
                     /gene="basR"
                     /locus_tag="OQS10_001756"
     CDS             9872..10540
                     /gene="basR"
                     /locus_tag="OQS10_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS2292795.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10541..11641
                     /gene="pmrB"
                     /locus_tag="OQS10_001757"
     CDS             10541..11641
                     /gene="pmrB"
                     /locus_tag="OQS10_001757"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS2292796.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(11617..11706)
                     /gene="pmrR"
                     /locus_tag="OQS10_001758"
     CDS             complement(11617..11706)
                     /gene="pmrR"
                     /locus_tag="OQS10_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS2292797.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(11818..13320)
                     /gene="proP"
                     /locus_tag="OQS10_001759"
     CDS             complement(11818..13320)
                     /gene="proP"
                     /locus_tag="OQS10_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS2292798.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13584..14450)
                     /locus_tag="OQS10_001760"
     CDS             complement(13584..14450)
                     /locus_tag="OQS10_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS2292799.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14447..16675)
                     /gene="crfC"
                     /locus_tag="OQS10_001761"
     CDS             complement(14447..16675)
                     /gene="crfC"
                     /locus_tag="OQS10_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS2292800.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17088..17423
                     /locus_tag="OQS10_001762"
     CDS             17088..17423
                     /locus_tag="OQS10_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS2292801.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17581..18024
                     /gene="yjdN"
                     /locus_tag="OQS10_001763"
                     /pseudo
     CDS             17581..18024
                     /gene="yjdN"
                     /locus_tag="OQS10_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18157..18945
                     /gene="phnC"
                     /locus_tag="OQS10_001764"
     CDS             18157..18945
                     /gene="phnC"
                     /locus_tag="OQS10_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS2292802.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            18970..19986
                     /gene="phnD"
                     /locus_tag="OQS10_001765"
     CDS             18970..19986
                     /gene="phnD"
                     /locus_tag="OQS10_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2292803.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20112..20891
                     /gene="phnE"
                     /locus_tag="OQS10_001766"
     CDS             20112..20891
                     /gene="phnE"
                     /locus_tag="OQS10_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS2292804.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            20912..21637
                     /gene="phnF"
                     /locus_tag="OQS10_001767"
     CDS             20912..21637
                     /gene="phnF"
                     /locus_tag="OQS10_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS2292805.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            21638..22090
                     /gene="phnG"
                     /locus_tag="OQS10_001768"
     CDS             21638..22090
                     /gene="phnG"
                     /locus_tag="OQS10_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS2292806.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22087..22671
                     /gene="phnH"
                     /locus_tag="OQS10_001769"
     CDS             22087..22671
                     /gene="phnH"
                     /locus_tag="OQS10_001769"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS2292807.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            22671..23735
                     /gene="phnI"
                     /locus_tag="OQS10_001770"
     CDS             22671..23735
                     /gene="phnI"
                     /locus_tag="OQS10_001770"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS2292808.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            23728..24573
                     /gene="phnJ"
                     /locus_tag="OQS10_001771"
     CDS             23728..24573
                     /gene="phnJ"
                     /locus_tag="OQS10_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS2292809.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24570..25328
                     /gene="phnK"
                     /locus_tag="OQS10_001772"
     CDS             24570..25328
                     /gene="phnK"
                     /locus_tag="OQS10_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS2292810.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25439..26119
                     /gene="phnL"
                     /locus_tag="OQS10_001773"
     CDS             25439..26119
                     /gene="phnL"
                     /locus_tag="OQS10_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS2292811.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26116..27252
                     /gene="phnM"
                     /locus_tag="OQS10_001774"
     CDS             26116..27252
                     /gene="phnM"
                     /locus_tag="OQS10_001774"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS2292812.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27252..27809
                     /gene="phnN"
                     /locus_tag="OQS10_001775"
     CDS             27252..27809
                     /gene="phnN"
                     /locus_tag="OQS10_001775"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS2292813.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            27796..28230
                     /gene="phnO"
                     /locus_tag="OQS10_001776"
     CDS             27796..28230
                     /gene="phnO"
                     /locus_tag="OQS10_001776"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS2292814.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
BASE COUNT         6803 a         7865 c         7311 g         6287 t
ORIGIN      
        1 aaaaaaacgc ggataaggaa ccagttgttc acgctcacgt ttatcgagcg tgatggttgg
       61 aaccagcgac cagaagggaa tatccataat ggtgtaagtc atgccccaga ggatgtaggt
      121 cacgcaaaca aagacaatct gagtagtacc ttcaaacaga tgcgcactaa agaggagaaa
      181 taagattaca gagtttgcca acgtaccgat caggatccag ggtttgaact taccccatcg
      241 cgatcgcgta gcatttacaa tccatcccat aatcggatcg ttaatagcat cccagatcct
      301 cgccaccaga aacaaagtac cgaccaaacc cacagacagc ccaacgacat cggtgtagta
      361 atacatgagg tacatataca caatgccgat cgcaaaatcc ttcccgaacg ctccaaatcc
      421 ataactgagt tttgtagtca ttgaaatgct catagggtat cgggtcgctg ttaccaacgc
      481 ccgccttctg ttattggtat cgtcagaggt gtgcgccgga ataaatcccc cggcgcagta
      541 gcgtttagtc gcgttttaac ggtgcaacca gcctggcagc cagtcgccgt gggcggcaat
      601 caggtcgtca acaagagcat atatttcgtc aatgcccagc acggcggcag tatgcgggtc
      661 catcatcgcg gcgtggtaaa cacggtcgcg attttccgta agaatagctt cggtcagcag
      721 cgtctgtaca ttgatgttgg tttgcatcag ggcggccaga tgcgaaggta gcgtaccgac
      781 tttagtcggc tgaatgccat tagcatcaac cagacaggct acttccacgc aacatccttg
      841 tggcaggtta tcaatcaaac catcgttacg gacgttgccg taaatcacac tcggctcgcc
      901 agtccagata gcgttcatga ttgtgctggc atattctcgt gacggtttaa tatcaatccg
      961 ggaggcgttt ttatactcct ccagctcttt atgccagttc gccagctgct cgacgcagcg
     1021 tttcgggtac tcatccagcg gtactttata acgctcaatc aaatcctcac gacctggctt
     1081 aataaaccac ggcgtgtatt cagcaaaatg ttctgacgat tccgtaacga agtagcccag
     1141 ctttttgaac atttcgtagc gcacaatatt ctggcagcga gtattaccat gaatatttgg
     1201 cttcggtgcc tgccctgctt cataagccgc cagcagttcc gggtagagat tcacgtaact
     1261 gccgtcggcg gttttacgct ccagttccag gtaaaacgcc atatggttga taccagcgca
     1321 acggtaacgc agcgtggacg gatcgatatt gaggtcacgc gccagctctt ccgccgttcc
     1381 ctgcaccgaa tggcacagcc ctacctgttt gatatgcaga tagcgggcat acatcgccca
     1441 ggtattcatc gccattgggt taacgtagtt gagcatggtg gcatcggggc agacttccgt
     1501 catgtcctcg caaatttgcc acagatgcgg aatggtacgt agcgcgcgca taataccgcc
     1561 cggccccaac gtatcggcaa tggtttgttc cagaccatgc cgcttacaga cctcgaaatc
     1621 agtcaccgtg caaggttcat aaccgccaat ctgaaatgcc accacgacaa aatcggcatc
     1681 ctgtaaggct tctttctgtt gggtgtggca ggtgattttg ccgctggccc ctgctgaatc
     1741 catcagctta cgcaccacaa tatgcgactc ttccaggcgg gtgggatcaa tgtccatcag
     1801 ggcaatatgc gccgttttca gcgcctcgcg atggaacaca tcaccaagaa tatttttaac
     1861 gaaaatcgtc gaaccagcgc cgataaatgt aattttgggt gcagtcatca tgcagatctc
     1921 ctggcttgct tgaataactt catcatggca ggcgaatctg cagaaaacct ccggcttcca
     1981 ggttgagcat tcccataaac tcagatcctg cgtgaagcag cagtaaatct gagtttatgg
     2041 taataagtta cctgaaaagc agagttttcg gcctcatgat gctcggacgc tcccctcgcc
     2101 tctctgggaa gagggtgaaa accgtgctcc cactcgcagt catcctccct cactcctgcc
     2161 ataattctga tattccagga aagagagcca tccatgaata cagatacgtt tatgtgcagc
     2221 agcgacgaaa aacagacccg cagcccgctg tcgctgtatt cggaatacca gcggatggag
     2281 attgagtttc gcgcaccgca tatcatgccc accagccact ggcatggtca agtcgaagtg
     2341 aatgtgcctt tcgatggcga tgtggaatac ctgatcaaca atgaaaaagt gaatatcaat
     2401 cagggtaata tcacgctgtt ctgggcctgt acaccgcacc aactaacaga taccggaacc
     2461 tgtcagagca tggcgatttt taatctgccg atgcatctgt ttctctcctg gccgctggat
     2521 aaagacctga ttaaccacgt cactcacggc atggtgatca aatcactggc cacacagcaa
     2581 cttagcccgt ttgaagtgcg ccgctggcag caggaattaa acagtccgaa cgagcaaatt
     2641 cgccagctcg ccattgatga aattggcctg atgctcaagc gatttagcct ctctggctgg
     2701 gaaccgattc tggtcaataa aacctcgcgc acacacaaaa acagcgtctc gcgccatgcg
     2761 caattttatg ttagccagat gctgggcttt attgccgaaa actatgatca ggcgctgacc
     2821 atcaacgatg tggctgagca cgtcaaactt aacgccaact atgcaatggg gatattccag
     2881 cgggtcatgc aattgacgat gaaacagtac attaccgcga tgcgcatcaa ccacgttcgc
     2941 gcgttactga gcgataccga taaaagtatt ctcgatattg ccctgacggc aggctttcgt
     3001 tcaagtagtc gtttttacag cacgttcggc aaatatgtcg gcatgtcgcc gcaacaatac
     3061 cgcaaactta gccaacagcg ccgccagacg tttcccggct aaagacgata tcagtatcag
     3121 ccaaaaaaat agtttagccg tcgataataa gcagtgaaaa taatttaaaa taatcacata
     3181 acgcattgtt tataaaaata aatatttcaa aaatgtttgt ttttcacgcg ctttacagcc
     3241 cgaaaaggcc ggaagatact tgcccgcaac gaagattcct tcataaccgg gtaagcaatg
     3301 atgaaagtat taattgttga aagcgagttt ctccatcaag acacctgggt cggtaacgcc
     3361 gttgagcgtc tggcagatgc tttaagccag caaaatgtta ccgtgattaa atccacctcc
     3421 tttgatgatg gttttgccat tctctcttca aacgaagcca ttgactgcct gatgttcagc
     3481 tatcaaatgg aacatccgga cgaacatcaa aacgtcagac aattgatcgg taagcttcat
     3541 gagcgccaac aaaacgtgcc ggtcttcctg ttgggcgatc gggaaaaagc cctcgccgca
     3601 atggatcgcg acctgctgga gcttgtcgat gaattcgcct ggattctgga agataccgcc
     3661 gactttatcg ccggacgcgc tgttgccgcg atgacccgct accgccagca gctgttgccg
     3721 ccactgttca gcgcgctgat gaaatatagc gacatccatg aatattcctg ggcagcgcca
     3781 ggccaccagg gcggcgttgg ttttaccaaa acacccgccg gacgtttcta ccatgactac
     3841 tatggtgaaa atctgttccg caccgatatg ggcatcgaac gaacttccct cggttctttg
     3901 cttgaccata ctggcgcatt tggcgaaagc gaaaaatatg ccgcacgcgt atttggtgcc
     3961 gatcgctcct ggtcggtagt cgtcggtact tccggctcta accgcaccat catgcaggct
     4021 tgcatgaccg ataacgatgt cgtggtcgtt gaccgtaact gccataaatc catcgaacaa
     4081 ggcttgatgc tgacaggcgc gaaaccggtc tacatggtgc caagccgcaa ccgctacggc
     4141 attatcgggc caatctatcc gcaggaaatg caacctgaaa ccttgcagaa gaaaatcagt
     4201 gaaagcccgc tgactaaaga caaagccggg caaaaaccgt cttactgcgt ggtgaccaac
     4261 tgcacctatg acggcgtgtg ttataacgct aaagaagcgc aggatctgct ggaaaaaacc
     4321 tccgatcgtc tgcactttga cgaagcctgg tacggctatg cacgtttcaa cccgatctat
     4381 gccgatcact atgccatgcg cggcgaacct ggcgatcaca acggtcctac cgttttcgcc
     4441 acccactcca cccacaaact gctgaatgcg ctttcacagg cttcttatat tcatgtacgt
     4501 gaaggtcgtg gggcgattaa cttctcccgc ttcaaccagg cctacatgat gcatgccacc
     4561 acctccccgc tgtatgccat ctgcgcatcc aacgacgtgg cggtgtcgat gatggacggc
     4621 aacagcggcc tgtcactgac gcaggaagtg atcgacgaag cggttgattt ccgtcaggcg
     4681 atggcgcggc tgtataaaga gttcaccgct gacggtagct ggttcttcaa accgtggaac
     4741 aaagaagtcg tcaccgaccc acaaaccggc aaaacctatg actttgctga cgcaccaacc
     4801 aaactgctga ccaccgttca ggactgctgg gtaatgcatc cgggcgaaag ctggcacggc
     4861 ttcaaagata ttccggataa ctggagtatg ctcgacccga ttaaagtcag catccttgct
     4921 ccgggaatgg gtgaagatgg tgaactggaa gaaaccggtg ttccggcggc gctggtcact
     4981 gcctggcttg gtcgccacgg cattgtgcct acccgcacca ctgacttcca aattatgttc
     5041 ctgttctcta tgggcgtaac ccgtgggaaa tggggaactc tggttaacac cctttgctcc
     5101 ttcaaacgcc actatgacgc caacacaccg ctggcgcagg tgatgccgga acttgttgaa
     5161 caatatcctg acacttacgc gaacatgggg attcacgatc tgggtgacac catgtttgcc
     5221 tggctgaaag aaaacaaccc tggcgcacgg ttgaacgaag cctattccgg cctgtcggtg
     5281 gcggaaatca ccccgcgtga agcgtacaac gcgattgtcg acaacaatgt cgaactggta
     5341 tccattgaaa atctgccagg acgcatcgcg gcaaactcag ttatcccata tccgccagga
     5401 atcccgatgc tgctgtctgg tgaaaacttc ggcgataaaa acagtccgca agtaagttat
     5461 ttacgctcgc tgcaatcctg ggaccaccat ttccctggat ttgaacacga aactgaaggg
     5521 actgaaatta ttgacggtat ttaccacgtt atgtgtgtga aagcgtaacc actattccgc
     5581 tgaaggcgta attgtttaaa taacattacg ccgcctggcc ttaggccttt tgagtatggc
     5641 aacgttttca taaaaattgc tgcaaacaaa aatgtcatac tttttgcgcg gccccacccc
     5701 gcgctttttt ttgcctgtta tttatcctgt aaaaaaatat gtacatgaga aattactata
     5761 aaaatttgta atattagtaa aactcgttat ttttatgcat gtttatattc atcatacaat
     5821 tatataacca tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc
     5881 aggagtacat gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa
     5941 gatgtctgga taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg
     6001 ttattaaatt gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct
     6061 catattagtc acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa
     6121 ataccagtat ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac
     6181 gtctttcgcg ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa
     6241 cgaacacgcg cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt
     6301 tcattaatga tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa
     6361 agcgatattc acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca
     6421 agtctgttaa agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc
     6481 tgccgcatgc gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta
     6541 tcacagtcct gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac
     6601 ggtatgacgc cgctgcatta tgttagtcag cacagagaac gaactgtcgc ctgattttta
     6661 accttaacga agagctatat taataacggc atcagcgata acccggtcga taataattca
     6721 actatcgaat gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag
     6781 gagtttaatt atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct
     6841 gatggtgtcg gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc
     6901 cactggcggg attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc
     6961 gatggtatac gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcttacgc
     7021 ccgccgctgc tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg
     7081 ctggatcggc aatatcgcta tggtggtcat tggcgtagga tatttaagtt acttcttccc
     7141 gattctgaaa gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt
     7201 cctgctgaac attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct
     7261 ggcgctgatc cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac
     7321 ctatatggcg gcatggaacg tcagcggcct gggcaccttc ggtgcgattc aaagtaccct
     7381 taacgttacg ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt
     7441 gaaaaacccg aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt
     7501 ttgctatgtg ctctccacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt
     7561 ttctgcctcg ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ctggggccat
     7621 tgtttccttc tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc
     7681 gggtcaaacg gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt
     7741 aaataaagcg ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt
     7801 ccagctcagt agcatttcgc caaacgcaac caaagagttc ggtctggtat cttccgtgtc
     7861 ggtcatcttt acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggcca
     7921 cggtcacttt ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta
     7981 ctgcatctgg gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct
     8041 gatggtcatc accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc
     8101 cttagatgca ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac
     8161 cttaagaact taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg
     8221 gaaacaccgt gatgttgaag cgcctactaa aaagcccctc tttgaattta ctcgcctggc
     8281 tattgttggc cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt
     8341 tgcaggcgct gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg
     8401 tcgctttcag cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac
     8461 cactggcctg cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg
     8521 gcatcgtcat cgaccgctcg atgattgcca atattattga taccactcca gcagaaagtt
     8581 atgcgctgat gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc
     8641 tgattgcctg ctggctaaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc
     8701 gtggagccaa tattctggtt tctgtactgc tgattttgct ggtcgccgca ctgttttata
     8761 aagactacgc ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta
     8821 acagcattgt tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg
     8881 tgcgaattgg tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt
     8941 tgaccatcct gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc
     9001 cgcgtgaaac taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat
     9061 cttgcggcac ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc
     9121 attacaaaga agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcaagcgg
     9181 gcatcaacgt gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc
     9241 ctcaccagaa cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg
     9301 acgaagtgct gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga
     9361 ttgtcttaca caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt
     9421 tcaggaaatt taccccaacc tgcgacacca atgagatcca gacctgtacc aaagagcaac
     9481 tggtgaacac ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta
     9541 atctgctgaa agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg
     9601 gtgaatcgtt aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg
     9661 atagccaaaa acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc
     9721 aggttgacca gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt
     9781 tattctccac gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg
     9841 atgttctgca aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac
     9901 gctgttattg cagggactga ttctggcggc gcaaaccgaa ggctacacgt gcgatggcgt
     9961 aacaaccgcg cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact
    10021 ggatttaggg ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa
    10081 atacaccctg ccggtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg
    10141 gctggatgtc ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc
    10201 ccgtatccgc gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg
    10261 caatctgacg ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct
    10321 gacgcccaaa gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtaca
    10381 tcgggaaatt ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct
    10441 ggaagtgcat atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg
    10501 cggctttggc tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcgttttct
    10561 gcgccgacca atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt
    10621 gtttgagctg atcagcatct tctggctatg gcatgaaagt accgagcaga ttcagctgtt
    10681 tgagcaggcg ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga
    10741 ggcggtcgcc agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg
    10801 ctatcaggcg gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc
    10861 gcgcaccgcc gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc
    10921 ggtggtttcg gcgttgaacg atctggtcag tcgcctgacg agcacgctgg ataacgaaag
    10981 gttatttacc gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca
    11041 tctggaactg ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga
    11101 tcagatgatg gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc
    11161 ttccggtaat tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga
    11221 actcagtacc atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga
    11281 catcaccgtt cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa
    11341 cgcccatcgt tacagcccac aaggcagcaa cattatgatt aagctgcaag aagatggcgg
    11401 agcggtcatg gccgttgaag atgaaggacc gggtattgat gagagtaaat gcggggagtt
    11461 gagtaaagcg tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat
    11521 tgtcagccgc attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac
    11581 ttccggcacg cgggcctggg tacggctgaa gaaagatcag tacgtagcaa accagatata
    11641 aagaaagctg ctgaccacca gcacactgaa cacggtagtt aaactttcat aaacacggtt
    11701 tttcattgca ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa
    11761 ccttaagagt tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta
    11821 ttcatcaatt cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat
    11881 ctcgtggtca atatcatcga ttttctgctc gatattatcg taatgctcga cgagaatttc
    11941 cttcgcttcc tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt
    12001 ctctttcatg gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc
    12061 aggcatcatc agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc
    12121 ggcaaccagc accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg
    12181 gaacatcgct ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag
    12241 catcagtaac ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg
    12301 gatcgccaac acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg
    12361 gtcactcagc aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat
    12421 aatcagcacc ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata
    12481 ggtcagcaac atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag
    12541 gctgcgccag tatttagtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc
    12601 ttcgcggtcg ccctgttcca gtttatcaac atgctgctgg aacgccggag tttcttccag
    12661 cgcatggcgc aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg
    12721 ccagccccag tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc
    12781 cgcacccagc acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg
    12841 tttacggtca ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac
    12901 cgagaaacct tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt
    12961 gtcgtaggac ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat
    13021 agcgaggatc ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag
    13081 cggtcgaatc agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg
    13141 gtcagccccc gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa
    13201 atcgaaccat tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc
    13261 gtcatcaata atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat
    13321 agctttcctc gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg
    13381 caaaagggga gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta
    13441 caccctccgg ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg
    13501 acctttttac cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag
    13561 gactcattga tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt
    13621 gcccccacca aacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac tgtttgccag
    13681 cgttgggtca ctgaccagca aacgggctgt ttatctgccg tggaccactt tcccagccaa
    13741 cttaaaaaat cagcatgctc ccctcccgaa gaggcaaacc actgatggta atagtgacgc
    13801 gtggtcggtg ccgcattgat actctctgcc agatgttgtg tcgctttctc gcgcaaatca
    13861 cccagtaaca acaccaccgc ttgcgggctt cccagacggc aggcatcgcc aaatgcaccc
    13921 cagcgcaact cctccagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc
    13981 ccagcacgcc agcgggtaaa aatttgctcg ctctgatcac ctgcctgcac cagtaggcct
    14041 cgttgcgtta acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg
    14101 gcaatttgct gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc
    14161 agcgccagat gctgcgcgat ctgcgtcagc gccagatgac cgggatccag catcgacgcc
    14221 agtttttcca ccattgtcag tcgcaggcta agcggcgcat ttaacatgcc gccgttcgtc
    14281 catgtctgaa gctgcacacg cgaaagaagt tcctgcatca gtcgttcacg aaactgctgc
    14341 tgatgggctt gtggaaggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga
    14401 taaaccgact ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg
    14461 ccgtgaaaag tgtttgaata tcatcgcgta acagctgggc gtcggtgtaa atccagccgt
    14521 gagtcatcac agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt
    14581 gacgcagagc caggctttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact
    14641 cagcgcaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt
    14701 tagccatttg ttggcaataa agatcgactt ctgcgcttaa agctcgttgc aacacactgt
    14761 aatcaaccgt ctctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc
    14821 agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcatcct
    14881 ggcgcgaaat gacttcactg aacagctggc gagtgttgac gtggggaact actccgtgaa
    14941 aaacaggaaa atgaacccca ggacgaaacc ctgactcgct caattccatt ttgacttgtt
    15001 gctcaatggg ccgaataaca tcggttaaca cccggcaaag ggtggattca agctcggcaa
    15061 aacgcagcgt aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc
    15121 aggtacgaat ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcgcat
    15181 tcagcgcatc ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt
    15241 catgtttaat ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt
    15301 cgacctgatg gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa
    15361 aatccaggta ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgca gcagatcgca
    15421 gagcatacaa cgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga
    15481 acagcgaatc ttcccacaac tgatcggcag catgacgaat atgttcgagg tccgccagat
    15541 cggcatgacg ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt
    15601 gctctggcgc tggtaactta ccgttgttgg ctaactcatg gcgcgctcga tttgccaagt
    15661 agccccacat cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg
    15721 tcccggaaat cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga
    15781 acttattgac cagcacatac agtggcaccg attgccccac cgccaaaatc gcctcacgga
    15841 cctcttcatc ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg
    15901 cgcgtgccag ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc
    15961 cgggggtatc cagtaacgtc aactgaccgg gataactctc cagccccgcc agatggacaa
    16021 actccacttc aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga
    16081 cgtccagcgc cttcgccagt cgcactaaat cattcaaact tttcagacaa tgaaaaatag
    16141 gctgggcacc cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg
    16201 cacgcatatc tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca
    16261 ggcgctgttg taattgttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta
    16321 ccggttcctt ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat
    16381 tacgattagg cagaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt
    16441 tcatagtacc gacaatcgcc agcaccattt ccagtcggga aattttactc aactcattat
    16501 tcagcatcgc gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg
    16561 cgtcgtctcc gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa
    16621 gctgtaacag gcgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa
    16681 attcttcctt aaagcaaatt ttgttacttt tatttagcca gattgttttt gagttctgtt
    16741 tgagttctgt ttttggcttt tataattact gcaagaaata attttatatt tagtgtgttg
    16801 ttttttatca gaataaataa cgtcttctga tgcgtttaaa acgtcagaaa gataaaaata
    16861 tcatgtgaat aaaaaaaaga acaagtagag cattaacatt atcttaaata ataaatagag
    16921 gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga aaacgatcgt cgtctaaaat
    16981 cagcagtacc cccgacaaac tcagggattt tgtgtataat tgcggccttt ttcggcaatc
    17041 tgccgttttt tggcgctttt gccctgctga cttttgagga aatccacatg tcattaccac
    17101 actgcccaaa atgcaactcc gaatacactt acgaagataa cggcatgtac atctgcccgg
    17161 aatgtgccta cgaatggaac gacgcagaac ctgcacagga aagcgacgag ctgatcgtta
    17221 aagatgctaa cggcaatctg ctggctgacg gcgacagcgt taccatcatt aaagatctga
    17281 aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa agtgaaaaac atccgcctgg
    17341 ttgaaggcga ccataacatc gattgcaaaa tcgacggttt tggtccgatg aaactgaaat
    17401 ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta agccggataa ggcgctcgcg
    17461 ccgcatccgg caacgatgcc gactgcctga tgcgacgctt gcgcgtctta tcaggcagcc
    17521 tctcatctgt ataaatttcg aactacactt aattggcttc tcttaactga ggtcaccatc
    17581 atgccgttaa gtccctacct ctcttttgcc ggtaactgtg ccgacgcaat tgcttattat
    17641 caacgtacgt tgggcgctga actgctctat aaaatcagct tcggcgaaat gccaaaatca
    17701 gcgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat ttcccgatac cgccatcgcc
    17761 cacgccaatg tgcgcattgc cggaagcgac atcatgatga gcgatgccat gccgtcagga
    17821 aaagccagct actccggctt tacgctggtg ctcgattcgc aacaggtcga agaaggaaaa
    17881 cgctggtttg acaatcttgc cgctaacgga aaaatcgaaa tggcctggca ggaaactttc
    17941 taggcgcatg gctttggcaa agtcaccgat aaatttggcg taccgtggat gattaatgtc
    18001 gtcaaacaac aaccaacgca ataacccgcc gggaggcccg ccctcccgca ctgtcatcga
    18061 attcccgtta actcttcatc tgttagtcac ttttaattaa ccaaatcgtc acaataatcc
    18121 gccacgatgg agccactttt ttagggaggc tgcatcatgc aaacgattat ccgtgtcgag
    18181 aagctcgcca aaaccttcaa tcagcatcag gcgctgcatg cggttgctct gaacattcat
    18241 cacggtgaaa tggtggctct gcttgggccg tcgggttccg gcaaatccac ccttttacgt
    18301 cacttaagcg gtttgattac cggcgataaa tccgccggca gccatatcga gctgctgggc
    18361 cgcacagtcc agcgcgaagg ccgtctggcg cgcgatatcc gcaaaagccg cgccaacacc
    18421 ggctacatct tccaacaatt caacctggtg aaccgcctga gcgtactgga gaacgtgctg
    18481 attggcgcgc tcggcagcac gccgttctgg cgcacctgtt ttagctggtt tacccgcgag
    18541 cagaaacaac gcgcgttaca ggcgctgacc cgcgttggca tggtgcattt tgcccatcaa
    18601 cgcgtttcca ccctctccgg cggacagcag cagcgtgtgg cgattgcccg cgcgctgatg
    18661 cagcaggcga aggtgattct ggccgatgaa cccatcgcct cgctggaccc ggaatccgcc
    18721 cgcatcgtga tggacaccct gcgcgacatc aatcagaacg acggcatcac cgtggtcgtc
    18781 acgctgcatc aggtggatta cgccctgcgc tactgcgaac gcatcgtcgc cctgcgccag
    18841 gggcacgttt tctacgacgg cagcagccaa cagtttgata acgaacgttt tgaccatctc
    18901 taccgcagca ttaatcgcat cgaagagaac gcgaaagctg cctgacatcc ccatcattga
    18961 ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct tcaccagcat gttcagcctc
    19021 agcaccctgt taagcccggc acacgccgaa gagcaggaaa aggcgctgaa tttcggcatt
    19081 atttcaacgg aatcacagca aaacctgaaa ccgcaatgga cgccattctt acaggatatg
    19141 gagaagaagc tgggcgtgaa ggtgaacgcc ttctttgccc cagactacgc aggcattatc
    19201 cagggaatgc gcttcaataa agtggatatc gcctggtacg gcaacctgtc ggcaatggaa
    19261 gcggtggatc gcgccaacgg ccaggtcttc gcccagacgg tcgcggcgga tggatcgcca
    19321 ggttactgga gcgtgttgat cgtcaacaaa gatagtccga tcaacaacct gaacgatctg
    19381 ctggcgaagc ggaaagatct caccttcggc aatggcgatc ctaactccac ctctggcttc
    19441 ctcgtccccg gttactacgt ctttgccaaa aacaatatct ccgccagcga ctttaagcgc
    19501 accgtcaacg ccgggcatga aaccaacgcg ctggccgtcg ccaacaagca ggtggatgtt
    19561 gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct ccgcgccaga gaagctgaaa
    19621 gaactgaagg tgatctggaa gtcgccgctg atcccaggcg atccgatcgt ctggcgcaag
    19681 aatctctccg aaaccaccaa agacaagatc tacgacttct ttatgaacta cggaaaaacg
    19741 ccggaagaga aagcggtgct ggaacgcctg ggctgggcac cgtttcgcgc ctccagcgac
    19801 ctgcaactgg tgccgattcg ccagctcgca ctgtttaaag agattcaggg cgtaaaaagc
    19861 aataacggac tgaatgagca ggacaagctg gcgaaaacca ccgagattca ggcgcagctg
    19921 gatgacctgg accgcctgaa caacgcgtta agcgcgatga gttcggtgag taaagcggtg
    19981 cagtaatgtg ctcgatcggt cccctctccc ttgagggaga gggttagggt gagggggaaa
    20041 tgcgcacgct gatgccctca ccccggccct ctcccacagg gagagggaga aaacattaaa
    20101 cggagccaaa catgcaaacc atcaccatcg ccccacccaa gcgcagctgg ttctcgcttc
    20161 tgagctgggc cattgttctc gccgtgttgg tcgtctcgtg gcagggcgcg gaaatggccc
    20221 cgcttacgct gatcaaagac ggcggcaaca tggcgacctt cgccgccgac ttcttcccgc
    20281 ccgatttcag ccagtggcag gattacctca ccgaaatggc cgtcacgctg caaatcgccg
    20341 tctggggcac cgcgctggca gtggttctct ccatcccctt cggcctgatg agcgccgaaa
    20401 acctggtgcc gtggtgggtt taccagcccg ttcgccgcct gatggacgcc tgccgcgcca
    20461 ttaacgaaat ggtcttcgcc atgctgttcg tggtcgccgt cggtctcgga ccgttcgctg
    20521 gcgtgctggc gctatttatt cacaccaccg gcgtgctctc gaaactgctt tccgaagcgg
    20581 tggaagcgat tgaacccggc ccggtggaag gcattcgcgc caccggcgcc aacaagctgg
    20641 aagagatcct ttacggcgtg ctgccacagg taatgccgct gctgatctcc tactccctct
    20701 atcgcttcga atccaacgtc cgctcggcga ccgtcgtcgg catggtcggt gcaggcggga
    20761 tcggcgtcac cctgtgggaa gcgattcgcg gtttccagtt ccaacaaacc tgcgccctga
    20821 tggtgcttat catcgtcacg gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc
    20881 actttatctg ataagtgagg cattgatatc tatgcacttg tctacacatc cgaccagcta
    20941 cccaacacgc tatcaggaga tagccgcaaa acttgagcag gagcttcgtc aacactatcg
    21001 ctgcggcgac tatcttcccg ccgagcagca actggcggcg cgctttgagg taaaccgcca
    21061 caccctgcgc cgcgccatcg accaactggt ggaaaaaggc tgggtacagc gccgtcaggg
    21121 catcggcgtg ctggtgctga tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt
    21181 tagccagaac ctgctggacc agggcagcca tcccaccagc gaaaaactgc tgtcggtatt
    21241 gcgccccgcg tccggtcacg ttgctgacgc gctggggatt accgagggtg agaacgttat
    21301 ccatttgcgc accctgcgtc gcgtcaacgg cgtcgcgctc tgtttaatcg accactactt
    21361 tgcggacctc accctctggc cgacactgca acgtttcgac agcggttcgc tgcacgattt
    21421 tctgcgcgag caaaccggaa ttgcgctgcg ccgcagccag acgcggatca gcgcccgccg
    21481 cgcccaggcc aaagagtgcc agcgccttga aatcccgaat atgtcgccgc tgctgtgcgt
    21541 gcgcaccctt aaccaccgtg acggtgaaag cagcccggcg gagtactccg tcagcctgac
    21601 gcgcgccgac atgattgaat tcactatgga gcactgaatg cacgcagata ccgcgacccg
    21661 ccagcactgg atgtccgtac tggcgcacag ccaaccggct gaactggcag cccgcctgaa
    21721 cgcgctgaac atcaccgccg actatgaagt gatccgcact gccgaaactg gcctggtgca
    21781 gattcaggcg cgaatgggcg gtaccggcga acgctttttt gccggcgacg ccacgctgac
    21841 ccgtgccgcc gtacgcctga ctgacggcac cctcggctac agctgggtgc tggggcgtga
    21901 taaacagcac gccgaacgct gcgcgctgat tgatgcgctg atgcagcaat ctcgccactt
    21961 tcaaaactta tcagaaaccc ttattgcccc gctggacgct gaccgtatgg cacgcattgc
    22021 cgcacgccag gccgaagtga acgccagccg ggtcgacttc tttacgatgg ttcgcggaga
    22081 caacgcatga ccctggaaac cgcttttatg cttcccgtgc aggatgccca gcacagtttt
    22141 cgtcgcctgt taaaggccat gagcgagccg ggcgtgattg tcgccctgta tcagctcaaa
    22201 cgcggctggc agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggccgataac
    22261 gacacgccgg tgtggcttgc cacgccatta agtaacgata tcgtcaatca gagcctgcgt
    22321 tttcatacca acgcgccgct ggtcagccag ccggaacagg cgaccttcgc ggtgacggat
    22381 gaggcgattt ccagcgaaca gctcaacgcc ctttccaccg acaccgccgt tgcgccggaa
    22441 gcgggcgcga cgctgatttt acaggtcgcc agcctgagcg gcggacgcat gttgcgtctc
    22501 accggcgcgg gcattgccga agaacgaatg atcgctccgc agctgccaga gtgcattctg
    22561 cacgaactca ccgagcgccc gcacccgttc ccgctcggca tcgacctaat cctgacctgc
    22621 ggcgagcgcc tgctggctat cccgcgaacc actcatgtgg aggtgtgctg atgtacgttg
    22681 ccgtgaaagg gggcgaaaag gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc
    22741 ggggcgatac cgatttgccc gaactgagcg tcgcccagat tgaacagcag cttaacctcg
    22801 cggtggatcg cgtgatgaca gaaggcggca ttgccgaccg cgaactggcg gcgctggcgc
    22861 tgaaacaggc cagcggcgat aacgtcgaag cgattttcct gctacgcgcc taccgcacca
    22921 cgctgacgaa actggcggta agcgagccgc tcgacaccac ggagatgcgt ctcgaacggc
    22981 gtatttcagc ggtttataaa gatattcccg gcggccagct gcttggccca acctacgact
    23041 acacccatcg cctgctcgat ttcactctgc tggcaaacgg cgaagcgccg acgctgacca
    23101 ccgccgacag cgaacaacag ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc
    23161 tggcgaagtt tgaagaggat agcggagcac agccggatga catcacccgc acgccgccgg
    23221 tttacccctg ttcacgttct tcccgtttgc agcagttaat gcgcggcgac gaaggctatt
    23281 tgctggcgct ggcttactcc acccagcgcg gttacgggcg caaccacccg ttcgcgggcg
    23341 agatccgcag tggttacatc gacgtgtcga ttgtgccgga agagctggga tttgcggtaa
    23401 acgtcggcga actgctgatg accgagtgtg aaatggtcaa cggttttatc gacccgccgg
    23461 atgagccgcc gcacttcacg cgcggctacg ggctggtatt cggcatgagc gagcgcaaag
    23521 cgatggcaat ggcgctggtt gaccgtgcgt tgcaagcccc ggaatacggc gagcacgcga
    23581 caggcccggc gcaggatgaa gagttcgtgc tggcgcatgc cgacaacgtc gaagccgcag
    23641 gctttgtctc gcacctcaaa ctcccgcact acgtcgattt ccaggccgaa ctggagctac
    23701 tcaaacgtct gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcctac
    23761 ctcgacgagc agaccaaacg catgatccgc cgcgccatct taaaagcggt ggcgatcccc
    23821 ggttatcagg taccgtttgg cgggcgcgag atgccgatgc cgtacggctg gggaactggt
    23881 ggtatccagc tcaccgccag cgtgattggc gaaagcgacg tgttgaaggt gattgaccag
    23941 ggtgcggatg acaccaccaa cgccgtgtcg attcgcaact tctttaagcg cgtgaccggg
    24001 gtaaacacca ctgaatgtac ggacgatgcg acgcttatcc agacgcgtca ccgcatcccc
    24061 gaaacgccgc tgaccgaaga tcagatcatt atcttccagg tgccaatccc ggaaccgctg
    24121 cgctttatcg agccgcgcga aacggagacc cgcactatgc acgcgctgga ggagtacggc
    24181 gtgatgcagg tgaaactgta tgaagatatc gcccgcttcg gtcatatcgc caccacctat
    24241 gcttatccgg tgaaggtgaa cgggcgctac gtgatggacc cgtcgccgat cccgaaattc
    24301 gataacccga aaatggacat gatgcccgcc ctgcagctgt tcggcgcagg gcgcgagaag
    24361 cgcatctatg cggtgccgcc gtttacccgc gtggaaagtc tcgatttcga cgatcacccg
    24421 ttcaccgttc agcagtggga tgagccatgc gccatctgcg gatcgaccca cagctatctt
    24481 gatgaagtgg tgctggatga cgccggaaac cgcatgtttg tctgctccga taccgattat
    24541 tgccgccaac agagcgaggc gaaaagccaa tgaatcaacc gttactttcg gtcaataacc
    24601 tgacccacct ttacgcgccg ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg
    24661 gggaagtgct gggcattgtc ggggaatccg gctccgggaa gaccacgctg ctgaagtcga
    24721 tctccgcgcg tctgacgccg cagcaggggg agattcacta tgaaaaccgt tccctctatg
    24781 caatgagcga ggccgaccgc cgtcgcctgc tgcgcaccga atggggcgtg gtgcatcagc
    24841 atccgctcga cggcctgcgc cgccaggtgt cggcaggcgg caatatcggc gagcggctga
    24901 tggcgaccgg ggcgcgacat tacggcgata ttcgcgctac cgcgcagaag tggctggaag
    24961 aggtggagat tcccgccaac cgtatcgacg acctgccgac caccttttcc ggcggtatgc
    25021 agcagcgttt gcagattgcc cgcaacctgg tgacgcatcc gaagctggtg tttatggatg
    25081 aaccgaccgg cgggctggat gtgtcggtgc aggcccgcct gctcgacctg ctgcgcggac
    25141 tggtggtgga gctgaacctc gcggtagtga ttgtcaccca cgatttaggc gtcgcccgcc
    25201 tgctggcgga ccgtttgctg gtgatgaagc aggggcaagt ggtggagagt gggttaaccg
    25261 accgcgtgct cgacgacccg catcatccgt atacacagct gctggtgtca tcggttttgc
    25321 agaattgagc cggtgccgga tgcggcgtaa acgccttatc cggcctacaa atgcgctccc
    25381 cgtaggtcgg ataagacgcg tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat
    25441 gattaacgta caaaacgtca gtaaaacctt catcctgcac cagcaaaacg gcgtgcgcct
    25501 gcccgtcctc aaccgcgcct cgctcatcgt caacgcgggc gaatgcgtgg tgctccacgg
    25561 ccattccggc agcggaaaat caaccctgct acgctcgctg tacgccaact atctgcccga
    25621 cgaagggcaa atccagatca aacacggtga cgaatgggtc gatctggtca ccgcgccagc
    25681 gcgcaaagtg gtggagatcc gcaaaaccac cgttggctgg gtgagccagt ttctgcgcgt
    25741 catcccgcgt atttcggcgc tggaagtggt gatgcagccg ctactcgata ccggcgtacc
    25801 gcgtgaagcc tgcgccgcta aagccgcgcg tctgctcacc cgcctgaacg tgccggaacg
    25861 cctgtggcat ctggcgccat cgaccttctc cggcggcgaa cagcagcgcg tcaacatcgc
    25921 ccgcggcttt atcgtcgact accccattct gctgctcgac gaacctaccg cctcgctgga
    25981 cgccaaaaac agcgccgccg tagtcgagct gattcacgaa gccaaagccc gtggcgcggc
    26041 catcgtcggc atcttccacg acgaagccgt acgtaatgac gttgccgacc gcctgcaccc
    26101 aatggaagcc tcttcatgat tatcaataac gtcaaattgg tgctggaaaa cgaagtggta
    26161 cacggttcac tggaggtgca ggacggcgaa atccgcgcct ttgccgaaag ccagagccgc
    26221 ctgccggagg cgatggacgg cgaaggcggc tggctgctgc cggggctgat tgagctgcat
    26281 accgataatc tggataaatt cttcaccccg cgcccgaaag ttgactggcc tgcccactcg
    26341 gcgatgagca gccacgacgc gctgatggtg gcgagcggca tcaccaccgt actggatgcc
    26401 gtggcaattg gcgacgtgcg cgacggcggc gatcggctgg tgaatctgga gaagatgatc
    26461 aacgccatcg aagagacgca gaaacgcggc gtcaaccgcg ccgagcaccg tctgcatctg
    26521 cgctgcgaac tgccgcatca caccacgctg ccgctgtttg aaaaactggt gcagcgcgag
    26581 ccggtgacgc tggtgtcgct gatggaccac tcgccgggcc agcgccagtt cgccaatcgc
    26641 gagaagtatc gcgaatatta tcagggcaaa tactccctca ctgatgcgca gatgcagcag
    26701 tacgaagaag agcaactggc gctcgccgca cgctggtcgc agccgaatcg cgaatccatc
    26761 gccgccctgt gccgcgcgcg aaaaattgcg cttgccagcc acgatgacgc cacccacgcc
    26821 cacgttgctg aatctcacca gcttggcagc gtgatcgccg aatttcccac cacgttcgaa
    26881 gcggcggaag cctcgcgcaa gcatggcatg aacgtgctga tgggcgcgcc gaatattgtg
    26941 cgcggcggct cgcactccgg caacgtggcg gccagtgaac tggcgcagct tggcctgctg
    27001 gatatcctct cttccgacta ctaccccgcc agcctgctcg atgcggcatt tcgcgtcgcc
    27061 gatgacgaga gcaaccgctt tacgctgccg caggcggtga agctggtgac taaaaatcca
    27121 gcgcaggcgc ttaatctcca ggatcgcggg gtgattggcg agggcaaacg cgcggacttg
    27181 gtgctggcgc atcgcaaggg caatcacatt catatcgacc acgtctggcg tcagggtaaa
    27241 agggtgttct gatgatggga aaactgattt ggttaatggg gccgtccggc tccgggaaag
    27301 atagcctgct ggcggaactc cgcctgcggg aacaaactca gctactggtg gcgcatcgct
    27361 acatcacgcg cgacgccagc gccggaagcg aaaaccatat cgctctgagc gagcaggagt
    27421 tttttacccg cgcggggcag aacctactgg ccttaagctg gcacgctaac ggtctgtatt
    27481 atggcgtcgg cgtcgagatc gacctctggc tgcacgccgg attcgacgtg ctggtcaacg
    27541 gctcacgcgc ccatctgccg caggcgcggg cgcgctatca atcggcgctg ctgcccgtct
    27601 gtttacaggt ttcgccggag atcctccgcc agcgcctgga aaaccgtggc cgtgaaaacg
    27661 ccagtgaaat taacgcccgc ctggcgcgcg ccgcccgcta tactccacag gattgccata
    27721 cgctcaacaa tgacggcagc ctgcgccagt cggtcgacac gctgctgacg ctgattcatc
    27781 agaaggagaa acaccatgcc tgcttgtgag cttcgcccgg ccacgcagta tgataccgac
    27841 gcggtttacg cgctgatttg tgagctaaaa caggcggagt ttgaccacca cgcatttcgc
    27901 gtgggcttta gcgccaatct gcgcgacccg aacatgcgct atcatctggc gctgctggat
    27961 ggcgaggttg tcggcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg
    28021 atcggcgaaa ttcaggaact ggtagtgatg ccgcaggcgc gcggcctgaa cgtcggtagc
    28081 aagttgctgg cgtgggcaga agaagaagcc cgccaggccg gggccgaaat gaccgagctt
    28141 tcgaccaacg tgaagcgcca cgacgcgcac cgtttctatc tgcgagaagg ctacgagcag
    28201 agtcacttcc gcttcaccaa ggcgctgtaa catgagtctg accctcacgc tcaccggcac
    28261 cggcgg
//
LOCUS       DAJVXP010000043        27467 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVXP010000043 DAJVXP010000000
VERSION     DAJVXP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.043"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..84)
                     /locus_tag="OQS10_001777"
     CDS             complement(<1..84)
                     /locus_tag="OQS10_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
                     /protein_id="HCS2292815.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLT"
     gene            complement(202..1641)
                     /gene="rsmF"
                     /locus_tag="OQS10_001778"
     CDS             complement(202..1641)
                     /gene="rsmF"
                     /locus_tag="OQS10_001778"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS2292816.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1722..4355)
                     /gene="yebT"
                     /locus_tag="OQS10_001779"
     CDS             complement(1722..4355)
                     /gene="yebT"
                     /locus_tag="OQS10_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS2292817.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4324..5607)
                     /gene="yebS"
                     /locus_tag="OQS10_001780"
     CDS             complement(4324..5607)
                     /gene="yebS"
                     /locus_tag="OQS10_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS2292818.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5737..6234
                     /gene="msrC"
                     /locus_tag="OQS10_001781"
     CDS             5737..6234
                     /gene="msrC"
                     /locus_tag="OQS10_001781"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS2292819.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6331..7029
                     /gene="proQ"
                     /locus_tag="OQS10_001782"
     CDS             6331..7029
                     /gene="proQ"
                     /locus_tag="OQS10_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS2292820.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7049..9097
                     /gene="prc"
                     /locus_tag="OQS10_001783"
     CDS             7049..9097
                     /gene="prc"
                     /locus_tag="OQS10_001783"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS2292821.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9289..10170
                     /gene="htpX"
                     /locus_tag="OQS10_001784"
     CDS             9289..10170
                     /gene="htpX"
                     /locus_tag="OQS10_001784"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS2292822.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10216..11279)
                     /locus_tag="OQS10_001785"
                     /pseudo
     CDS             complement(10216..11279)
                     /locus_tag="OQS10_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11456..12247
                     /gene="kdgR"
                     /locus_tag="OQS10_001786"
     CDS             11456..12247
                     /gene="kdgR"
                     /locus_tag="OQS10_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS2292823.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12390..12629)
                     /locus_tag="OQS10_001787"
     CDS             complement(12390..12629)
                     /locus_tag="OQS10_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292824.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12788..12931
                     /gene="mgrB"
                     /locus_tag="OQS10_001788"
     CDS             12788..12931
                     /gene="mgrB"
                     /locus_tag="OQS10_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS2292825.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13006..13293
                     /locus_tag="OQS10_001789"
     CDS             13006..13293
                     /locus_tag="OQS10_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS2292826.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13963..14106
                     /locus_tag="OQS10_001790"
     CDS             13963..14106
                     /locus_tag="OQS10_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS2292827.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14119..14328
                     /gene="cspE"
                     /locus_tag="OQS10_001791"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14119..14328
                     /gene="cspE"
                     /locus_tag="OQS10_001791"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS2292828.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14494..15303
                     /gene="rlmA"
                     /locus_tag="OQS10_001792"
     CDS             14494..15303
                     /gene="rlmA"
                     /locus_tag="OQS10_001792"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS2292829.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15300..15866)
                     /gene="mntP"
                     /locus_tag="OQS10_001793"
     CDS             complement(15300..15866)
                     /gene="mntP"
                     /locus_tag="OQS10_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS2292830.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16295..16753)
                     /locus_tag="OQS10_001794"
     CDS             complement(16295..16753)
                     /locus_tag="OQS10_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS2292831.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16808..17659)
                     /gene="manZ"
                     /locus_tag="OQS10_001795"
     CDS             complement(16808..17659)
                     /gene="manZ"
                     /locus_tag="OQS10_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS2292832.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17672..18472)
                     /gene="manY"
                     /locus_tag="OQS10_001796"
     CDS             complement(17672..18472)
                     /gene="manY"
                     /locus_tag="OQS10_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS2292833.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18535..19506)
                     /gene="manX"
                     /locus_tag="OQS10_001797"
     CDS             complement(18535..19506)
                     /gene="manX"
                     /locus_tag="OQS10_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS2292834.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19969..21519
                     /gene="yoaE"
                     /locus_tag="OQS10_001798"
     CDS             19969..21519
                     /gene="yoaE"
                     /locus_tag="OQS10_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS2292835.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21523..23121)
                     /locus_tag="OQS10_001799"
     CDS             complement(21523..23121)
                     /locus_tag="OQS10_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS2292836.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23252..24616)
                     /gene="sdaA"
                     /locus_tag="OQS10_001800"
     CDS             complement(23252..24616)
                     /gene="sdaA"
                     /locus_tag="OQS10_001800"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS2292837.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24800..25378)
                     /locus_tag="OQS10_001801"
     CDS             complement(24800..25378)
                     /locus_tag="OQS10_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS2292838.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25382..26743)
                     /gene="pabB"
                     /locus_tag="OQS10_001802"
     CDS             complement(25382..26743)
                     /gene="pabB"
                     /locus_tag="OQS10_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS2292839.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26817..26996
                     /locus_tag="OQS10_001803"
     CDS             26817..26996
                     /locus_tag="OQS10_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS2292840.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27116..27439)
                     /locus_tag="OQS10_001804"
     CDS             complement(27116..27439)
                     /locus_tag="OQS10_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS2292841.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7066 a         6768 c         6939 g         6694 t
ORIGIN      
        1 ggttaacgtt cgatcacccg gcgattcacc gtgtaattcg ccgtcgtcga tttcaaaagc
       61 gcctatgcgc acactggttt tcatcttcgt gctctccatt tagctgccgg ttgtggtacg
      121 accagtccga tcgcaccatt tttcagccta gttgaggcag ccgaatgtgc cagtcaaaaa
      181 gtgcgctttt tttgtgcgct gtcaggcgtt accggtaaaa agtttcccat cgcgcaccag
      241 ttcacgcgga tagctgtttt tcaatcgcga accaatccgt ttggctaaac caatcggttg
      301 atgctggaaa gtaaccaaca cgtcatccgc cactggcgcg gcttgcgggt aaacatcgcg
      361 cccgcgatac cactcctccg cttcctgcgg tgtcagttca aaagcgttca cattgtcggg
      421 ggtggcaagg gcaataactg cttcatgctg ccagcgataa cctttgttgt gcgtttcggc
      481 aagtttaatc cccaagcgag aaaatcggac tttaccgatc agggcttcaa tgcccaccgg
      541 gaacaaccac agttctttgt cacgctgcca gaggcgcagg ttttcatccc agtttaagcc
      601 aacacctgca gccgcctgac gaatttgtcc agcttctcga tctttcactg ggctgaacgg
      661 aaaattaccg actttgtatt tgggggcggg taaggcggga atcgcctgag ttttacgcag
      721 acgagcaacg aagaagcctt cgcagtcgta aatttgtggg aaaacatgca aaaagccttc
      781 ctcggtcagc gccttgtttg caccagggaa gagatcacca agcggtaaaa actctactgc
      841 gtcggggtaa gtctctttca gccacaggca aacggcttcg ttttcttcct ggtttaaggt
      901 acaggtcgag taaaccagcg taccgccagg gcgtaatgca tgaaaggcgc tgtcgatcag
      961 ttcccgttgg gtagctgcga tttcctgatt gctttctggt gaccagtttt ttagcgcatc
     1021 gggatcttta cgcaccacgc cttcgccgga gcagggagcg tccagcaaaa tggcatcaaa
     1081 catttctggc actgccgcac caaacacgcg gccatcaaaa tgtgtgagcg caacattact
     1141 gataccacag cggctgatat tggcatgtaa cacttttacc cgactggcgg aaaactcatt
     1201 ggcaagaatt gccccttcgt tattcatccg cgcggaaatt tgcgtcgttt tggagccagg
     1261 cgcggcagcg acatccatca cccgctgtgg tgcattaccg tcagcaaaca aggcggcgac
     1321 gggcaacatt gaactggctt cctgaatata aaacaggcca cttaaatgct cggcggtact
     1381 acccaatggc aatgcatctt cattgtcgcg ttcaatccag aaaccttctt cacaccacgg
     1441 aattggcgta agcgtccagc cataaggagc ggttaattgc aggaaatcag caacggagat
     1501 tttcagcgta ttaacgcgaa tgctgcggcg caacgggcgc tgacaggcgg caagaaaatc
     1561 atcaaaagag agcgtcgaag gcatcgcttc gcgcatttgc gtcagaaagg cgtccgggaa
     1621 ataaacggtg tgttgggcca cgagcatata ccactggcaa aaaaaacagg cgcgcagttt
     1681 agcataaacg ctccggcgca ggcacgccgg agcagtgggc attatttggg aagcgcagta
     1741 ccccattcac gccactcttt cggttcactt tcctgcaaca ggaagtgctt gccttcctgg
     1801 gctttcggtg ccagtggcgt acccggaggc gtggcgaagg cgatgccgcc acggataaac
     1861 tggttaaagg tgccggtttt cactacgccg cccgtcagac caaagtccag actgtaaccc
     1921 gatgccaacc agaagacgga attgttacgc accaggtgtt gatagcgttt actgatgcgc
     1981 atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca ttccggtaac cgtacctact
     2041 tccagaccac ggaacagcac aggcgtacca atacctaacg aaccggcttc cggtgcttcg
     2101 acaataatgc ttaagccatc caggtaacgc gaatcagtaa tagtagcttc ttgcaactca
     2161 aagtcacggc gaggattacc ccgacctggt tcgacattga tatacggctg gaggatagta
     2221 tctagatgct caacgcccgc ggctgaaatt tgcggtgtga ccaccgagaa gcgcgtaccg
     2281 ccgcgggcga aggtctggac atactccgga tagagcaccg cctttgcctg tacttcattg
     2341 cgcgcggtaa tcagatccag cgtctggatt tgcccgatat caataccgag atagcgaatt
     2401 ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa gcgtaatctg cccaccaacc
     2461 gcacgggccg ctgtttcgga agcatacaga attcgtttgt cgcctttacg ctgactggcg
     2521 ctggcaccgc tgaggttatc gaagctaatg gctcccttta atgctctgga gagcggggat
     2581 gcctgtacgg tcaggccact accattcagc tgaactttcg ccccgccttc tgcccagaac
     2641 acgctattgc tggtcagaag gttgcgatac tccggcttaa tatgcagatc gatatcaaac
     2701 gcgttagctc gcggacgcac ggtaatcact tcaccaactt caaatttacg gtacagcact
     2761 accgatcctg cctgcacatc cggcagcgtc tctgcactca aactcacggt tgtggtgggt
     2821 aaatcgctaa ggctgttctc cagcgctttt tccagattgg catacagtgg atagctggct
     2881 ttcatctcac ctttatcgcc tggcagaata cgtatcccgc cgttaatcca ttctgaggcg
     2941 ctggcaccaa gaaactcaac gccatccagc cccaccttca cgtcgacacg gctgttgacg
     3001 acaaatttgc tatcgccttt taccagttcg cgatgctgag gctcgatggc gacggtaaag
     3061 gtaacgcctt tgctggtgag tttacgatca ataacctggc ctacctgcac gccgtgaaga
     3121 atgagcggct gaccagcatc aataccgtaa ctttccggcg cggtcagggt cagcgtcaga
     3181 acatcaggtt catgcagcag tgctttttcg cctggcacaa caacgaactc tttgcgtggc
     3241 tcgccatcgc cgggtaccaa ctcgaaggtt ttgccagtca gcagggcgct gagattggca
     3301 tcgctaaggg ataatttcgg attaagtaat tcgatgcggg tattttcacg aagcagggta
     3361 acgacgctgg gattaactgt catttcccca gtgactttac caccaggatt taaatccagt
     3421 ttagttagct gtccgacttc cagtccctga tacattaatg gcgtcgagtc ggcggttaat
     3481 ccggccccac tcggcagttc cagtttgatt attacgccac gctggctatg ggcgaggtct
     3541 tcatacagac caaaggtatc ttccgcctcg gcaggtttcg actcttcagg tgaatcgaag
     3601 gcaatcgcac cgttaaccag tgccgccaga ctttccagtt tcaccttcgc accactgata
     3661 ctgacgttgg catcaacgcc ggaaacgttc cagaaacggc tacctttttt cactaggtcg
     3721 gtaaaacgcc gctcgatcag gacatcaatc accacgcctt gcttgttggg attgatggca
     3781 tagtcgtaga cttttcccac agggatcttg cggaaataga ccaatgaacc gctgttcagc
     3841 gaaccgagat cgggggcttg caggtggatc atcagatcgc cattgtccag tcgatatttc
     3901 ggttgggtat cgagtgcgac aaagtgatcc tgctctttac ctttacctgg catcatgccg
     3961 atatagttac caccgacgag ggcgtccagc ccggagacac ctgccaacga tgcttttggc
     4021 gtcaccagcc agaactgagt ctcttcgcgc agcgcatctt tcatatcgga cttgatgctg
     4081 accttgactt caatcttacg aagatcgtcg ctgaggctga tatcctgcac tgttccgact
     4141 tcaacgccct gataacgaac aggcgtacgg cccgggacaa taccatccgc cgacataaag
     4201 tcgatggtga cggtattacc ccggtcctga taactgtccc aaatcagcca accggcaatc
     4261 attagcgcga tgaaaggcag cagccagaaa ggtgagatac ggcgtttatt tttaatctgc
     4321 gcttcagtcg tcgaagcggg cgtttcctga ctcatgtgca tcccaaagta agcggctgtc
     4381 cagccattcc acagcaagaa tagtcaaaat tacggctgcg ccaaaataaa acgcagccgg
     4441 tcccatagta aaagcgagga tctgatcgcg attaatcagc gacatggtta aagatatgac
     4501 aaacaggtcg agcatcgacc agcgtccaat ccaggtcacc atccgcagta acagaatgcg
     4561 tgtgcgtaaa ccttgctggc atttaaaatg aatgctcaac agtaaagtga acatgacgat
     4621 cactttagta aacggtacca gaatactggc gataaacacg attccggcga ccgcaatgtt
     4681 gctacttgcc agcgacataa tcccggaaag aatcgtatct tcctgccggc caccattcag
     4741 ataaatgata gaaataggca ataggttggc tggtaacaac aaaacgattg acgctaacag
     4801 cgccgcccag catttttgca aactatgacg acgacgcagg cgtagcggga tatggcaacg
     4861 cgggcagcga ccacgctgat caggatagcc ggtaaaatgg cacccaagac agacacgaag
     4921 tttctcgtcc ctacgcgtag cggggcgctg cggataatat cgctcccaca gctcctcaac
     4981 attaagatgt gacaacgtca ccgtcgttaa aatcaccaac gccacaaaag agaacaagcc
     5041 gacacccgcc tggatatggg cataatcctg tacctttata gaagcaacgc caatgccgac
     5101 caggtagata tccagcatta cccactcttt aagacgctca agcatcagca gtaccggacg
     5161 taaattcatt cccagtcggt taccaaacca taaatatgct atggaggaca ccagaatgag
     5221 gggggcacca ataacgcaga aaaagaccat cgcccccgtt atcgtatcgc cctgtttggt
     5281 catttgccag atgccttgca tgacgttggc gtcgatacga atacctaaca gccagatatg
     5341 caacagcggt tcgccccagg caaacggcat cagcaacagc atggtgaaag ccattgccgc
     5401 caggcgcgtt agcgaccagt cgcgcccgtc gcgaattttt gcctgacagc gcggacaata
     5461 ggcactctga tgagaattta tctcgggcag gctaaacagc atgtcacatt gcgggcaacg
     5521 ttggtaatca ccacgcggca gttcctcgcc gattgccctc actgttatct tttttgtcgg
     5581 cgtaatttgt ggtgtgttaa gagccatgac gtgccaaatg taataagaat taacattaat
     5641 cttaacccat gatgggaatg atcttgagca ttaacgcatt taatgcttat tttaataggc
     5701 tggcagttcc aggtaagaca aacaagtgat tatataatga acaaaacaga attttacgcg
     5761 gatttaaatc gcgactttaa cgcgctgatg gcgggagaaa ccagttttct ggcaacgctt
     5821 gcgaacacca gtgcgttgtt atacgagcgt ctcactgacg taaactgggc aggtttttat
     5881 ttgcttgagg acgatacact ggtactcgga ccatttcagg gcaaaattgc ctgtgtccgg
     5941 atacccgtcg ggcgcggggt gtgcggcact gcggttgccc gcaatcaagt gcagcgtatc
     6001 gaggatgttc acgcgtttga cgggcatatt gcctgtgatg cggcgagtaa ttctgaaatt
     6061 gttctgccgc tggtggtgaa aaatcagatt attggtgttc ttgacatcga tagtaccgtc
     6121 ttcggtcgct ttacagacga ggacgagcaa ggcttacgtc agcttgtggc acagcttgaa
     6181 aaagtgcttg caacgacgga ttacaaaaaa ttctttgcga gcgtcgcagg ataatcaacg
     6241 gataacgtag catttactgg tagcgtcatt ataatgacgc ctgttcatgc ctgcgcttgt
     6301 tggctacgtc cgttgtaatc aggaaatttc atggaaaatc aacctaagtt gaatagcagt
     6361 aaagaagtaa tcgcgtttct ggccgaacgt tttccccact gtttcagtgc ggaaggtgaa
     6421 gcgcgtccgc tgaaaatcgg tatttttcag gatttggtcg atcgtgttgc cggggaaatg
     6481 aacctgagca aaacgcaatt acgatccgct ttacgtctct acacttcgag ctggcgttat
     6541 ctttacggtg ttaaacccgg cgcaacgcgt gtcgatcttg acggcaaccc atgcggtgag
     6601 ctggacgagc aacatgtaga gcatgctcgc aagcagcttg aagaagcgaa agcgcgtgtt
     6661 caggcacagc gtgctgaaca gcaagcgaaa aaacgcgaag ctgccgcagc tgctggtgag
     6721 aaagaagacg cactgcgccg cgaacgcaag ccacgtccga ccacgccacg ccgcaaagaa
     6781 ggcgctgaac gtaaacctcg tgcgcaaaag ccggtagaga aagcgccaaa aacagtaaaa
     6841 gcacctcgcg aagaacagca caccccggtt tctgacattt cagctctgac tgtcggacaa
     6901 gccctgaagg tgaaagcggg tcaaaacgcg atggatgcca ccgtattaga aatcaccaaa
     6961 gatggcgtcc gcgtccagct gaattcgggt atgtctttga ttgtgcgcgc agaacacctg
     7021 gtgttctgaa acggaggccg ggccaggcat gaacatgttt tttaggctta ccgcgttagc
     7081 tggcctgctt gcaatagcag gccagacctt cgctgtagaa gatatcacgc gtgctgatca
     7141 aattccggta ttaaaggaag agacgcagca tgcgacggta agtgagcgcg taacgtcgcg
     7201 cttcacccgt tctcattatc gccagttcga cctcgatcag gcattttcgg ccaaaatctt
     7261 tgaccgctac ctgaatctgc tcgattacag ccacaacgtg ctgctggcaa gcgatgttga
     7321 acagttcgcg aaaaagaaaa ccgagttagg cgatgaactg cgttcaggca aactcgacgt
     7381 tttctacgat ctctacaatc tggcgcaaaa gcgccgtttt gagcgttacc agtacgcttt
     7441 gtcggtactg gaaaagccga tggatttcac cggcaacgac acttataacc ttgaccgcag
     7501 caaagcgccc tggccgaaaa acgaggctga gttgaacgcg ctgtgggaca gtaaagtcaa
     7561 attcgacgag ttaagcctga agctggcagg aaaaacggat aaagaaattc gtgaaaccct
     7621 gacacgccgc tacaaatttg ccattcgtcg tctggcgcaa accaacagcg aagatgtttt
     7681 ctcgctggca atgacggcgt ttgcgcgtga aatcgacccg cataccaact atctttcccc
     7741 gcgtaatacc gaacagttca acactgaaat gagtttgtcg ctggaaggta ttggcgcagt
     7801 gctgcaaatg gatgatgact acaccgttat caattcgatg gtggcaggtg gtccggcagc
     7861 gaagagtaaa gctatcagcg ttggtgacaa aattgtcggt gttggtcaaa caggcaagcc
     7921 gatggttgac gtgattggct ggcgtcttga tgatgtggtt gccttaatta aagggccgaa
     7981 gggcagtaaa gttcgtctgg aaattttacc tgctggtaaa gggaccaaga cccgcactgt
     8041 aacattgacc cgtgaacgta ttcgtctcga agaccgcgcg gttaaaatgt cggtgaagac
     8101 cgtcggtaaa gagaaagtcg gcgtgctgga tattccgggc ttctatgtgg gtttgacaga
     8161 cgatgtcaaa gttcaactgc agaaactgga aaaacagaat gtcagcagtg tgatcatcga
     8221 cctgcgtagc aatggcggtg gggcgttgac cgaagcggta tcgctctcag gtctgtttat
     8281 tcctgcgggt cccattgttc aggtccgcga taacaacggc aaggttcgtg aagacagcga
     8341 taccgacggg caggtgttct ataaaggccc gctggtggta ctggttgacc gtttcagtgc
     8401 ttcggcttca gaaatctttg ccgcggcaat gcaggattac ggtcgtgcgc tggttgtggg
     8461 tgaaccgacg tttggtaaag gcaccgttca gcaataccgt tcattgaacc gtatttacga
     8521 tcagatgtta cgtcctgaat ggccagcgct gggttctgtg cagtacacga tccagaaatt
     8581 ctatcgcgtt aacggcggca gtacgcaacg taaaggcgta acgccagaca tcatcatgcc
     8641 gacgggtaat gaagaaacgg aaacgggtga gaaattcgaa gataacgcgc tgccgtggga
     8701 tagcattgat gccgcgactt atgtgaaatc aggcgattta acggcctttg ggccggagct
     8761 gctgaaggaa cataatgcgc gtatcgcgaa agatcctgag ttccagaaca tcatgaagga
     8821 tatcgcgcgc ttcaacgcta tgagggacaa gcgcaatatc gtttctctga actacgctgt
     8881 gcgtgagaaa gagaataatg aagatgatgc gacgcgtctg gcgcgtttga acgaacgctt
     8941 taaacgcgaa ggtaaaccgg agttgaagaa gctggatgat ctaccgaaag attaccagga
     9001 gccggatcct tatctggatg agacggtgaa tatcgcactc gatctggcga agcttgaaaa
     9061 agccagaccc gcggaacaac ccgctcccgt caagtaatat caatcaggca caagaaattg
     9121 tgcctgattt tttaacagcg gcaagatgcc gtaaatcaga tgctacaaaa tgtaaagttg
     9181 tgtctttctg gtgacttacg cactatccag acttgaaaat agtcgcgtaa cccatacgat
     9241 gtgggtatcg catattgcgt tttgttaaac tgaggtaaaa agaaaattat gatgcgaatc
     9301 gcgctcttcc tgctaacgaa cctggccgta atggtcgttt tcgggctggt actgagcctg
     9361 acagggatac agtcgagcag cgttcagggg ctgatgatca tggccttgct gttcggtttt
     9421 ggtggttcct tcgtttcgct tctgatgtcc aaatggatgg cattacgatc tgttggcggg
     9481 gaagtgatcg agcaaccgcg taacgaaagg gaacgttggc tggtcaatac tgtagcaacc
     9541 caggctcgtc aggcagggat cgctatgccg caagtggcta tctaccatgc gccggacatc
     9601 aacgcttttg caaccggtgc gcgccgtgat gcctctctgg ttgctgtcag caccggtttg
     9661 ctgcagaaca tgagcccgga tgaagccgag gcggtaattg ctcacgaaat cagccacatc
     9721 gccaatggtg atatggtcac catgacgctg attcagggcg tggtgaacac cttcgttatc
     9781 tttatttccc gtattctggc gcagcttgcc gcgggtttta tgggcggaaa tcgtgatgaa
     9841 ggtgaagaga gcaacggcaa cccgctgatc tactttgcgg ttgcaacggt tctggaactg
     9901 gtgtttggta ttctggcgag cattatcacc atgtggttct cgcgtcatcg tgaattccat
     9961 gctgatgccg gttcggcaaa actggttggt cgcgagaaaa tgattgccgc gctgcagcgc
    10021 ctgaaaacca gctatgaacc gcaagaagca accagcatga tggctttctg cattaacggt
    10081 aagtcgaaat cgctcagtga gttgttcatg actcacccgc cgctggataa acgaattgaa
    10141 gctctgcgta cgggtgaata cctgaagtaa tatgaagtaa atactaaaaa gcgcgtcctg
    10201 atcgacgcgc tttttttatg ccttggatcg tggctgagtg atacgtaaac cactgacaca
    10261 ggcagcaatc actgccagaa tagccgcagc catcagcgaa acgtgcgtac cattatcacc
    10321 gaactgattt agcatcagcg ccaccagtgc cgcgccgcta ctctgaccca gtagacgagc
    10381 cgttcctaac atgccactgg ctccaccgct acgttcgcga ggagcggagg taataatggt
    10441 gtggttattg ggtgactgga acaagccaaa tccagcgcca cataagatca tcggccagat
    10501 aatattgata tccgcaggtg acgcgggcag cagaaccagg ggaaaaagcc ccgcagccat
    10561 gatgaacaac cctagagcac ccagcaatcc tgcatgtacg cgttcaatca aatagcctgc
    10621 caacggagcc atcaccatcg ttgctaacgg ccacggtgtc agaagtaaac ctgtttcgac
    10681 ttcactacgc ccgagcacgg tttgcaggta aaagggcagg gaaaccattg ccagcatttg
    10741 tgcgcagaaa gagcaaacag atgtgcaaat agaaagtgaa aacagcggga tacgcagtaa
    10801 atccaccggt agcagcggta cgggaagaga aagctggcgg cgaataaaga aaataccaac
    10861 aacaaccatt accaccagtt ccgcagcaat taacgtcagc gattgcccct gagcgaaacc
    10921 actcaacgca gtgataagca ggccgaaggt taacgcgttc atcacggcgc tgggcaggtc
    10981 gaaacggggt ttactggcgc gagaaccatt gggtggcaga aaacgcatcg ccagaagcag
    11041 ggcgataata cctaacggta cgttgattaa aaataaccat ttccaggatg cgatggagag
    11101 gattgctgca gcaattgtcg gcccggcagc agaagagacg gcaacaatat ggcgattgtt
    11161 ggcagggcga cgttggcgat tgcgccgtca aggacagcca tcgaaatgcc aatcacaatg
    11221 gttaatatcg caccgtatcg ctggggcaat ggcaggccgt cggcctgaac ttttggcata
    11281 ggaattttat atctttggtg aataatcaac aggggtatga ttctagcact gatattcacg
    11341 gtggatgtcg cagaattgta actaaccggc aaaagatgga ttgcgaccac cctggtgcga
    11401 aattataata aaaaacagtt ctgattttta taaaacactc gcaatgaggt gataaatggc
    11461 taacgcagat ctggataaac agcctgattc tgtatcttcc gtgctaaaag tttttggcat
    11521 tttgcaggcg ctgggtgaag agcgcgaaat agggataacc gagctgtcgc agcgcgtcat
    11581 gatgtcaaaa agcaccgttt atcgcttttt acagaccatg aaaaccttag gttatgtggc
    11641 gcaggaaggg gagtcggaga aatattccct gaccctgaaa ttgtttgaac tgggcgctcg
    11701 cgcgttacaa aacgtcgatt taattcgtag cgcagatatc cagatgcgtg agatctcccg
    11761 cctgaccaaa gaaactatcc acctcggcgc actggacgaa gacagtattg tttacattca
    11821 caaaattgac tctatgtaca atttgcgcat gtattcacgg attgggcgtc gtaatccgct
    11881 gtacagcacc gcgattggta aggtactgct ggcatggcgc gatctcgatg aagtgaagca
    11941 aattcttgag ggcgtggagt ataaacgcag taccgagcgg accatcacca gtacagaagc
    12001 gttattaccc gttctggacc aggtgcgcga gcaggggtat ggcgaagata acgaagagca
    12061 ggaagaaggg ctgcgatgca ttgcggtacc ggtatttgat cgctttggcg tggtcattgc
    12121 cggtttgagc atctccttcc cgacgttgcg tttctctgaa gagcgtttac aggaatatgt
    12181 cgcaatgttg cataccgcag cgcgcaaaat ttctgcccaa atgggttatc acgactatcc
    12241 gttctgatga gagtaagaac ctgtcggaat atcaaacaga caggttcttt atttagcatg
    12301 agaaaaataa agttgaaggt gcgttatatt aaacgcgctt gctataagaa tattttactc
    12361 atgagtgaga atctggttgt ttattaccct taaccattat cgaccacgat attgctttta
    12421 cgtaacagtg ggcaatctgt tatccccaag aaaccacttt tagtgtgcaa gtattgtacc
    12481 gtgctggtgc ctctggcagt cagataggta cattgcaaac ctaatcctgc ggcattctct
    12541 ttgcttccaa tcaaaacgcc atatccgctg agtaataatc ctatccatac cagtgctatc
    12601 agcataactg tgcgaatgat gaatcgcatt acaacctctt ctctttttat gttcgcttaa
    12661 tcgtagcggc aatatgcgct gaagcaagct actcattccg aaaaagcacg aatatcgaca
    12721 tagttaggcg ctgtttaact aacgcatgct agtttaatga cataaggtag gtgaagcgga
    12781 gattggagtg aaaaagtttc gatgggtcgt tctggttgtc gtggtgttgg cttgcttgct
    12841 gctttgggcg caggtattca acatgatgtg cgatcaggat gtacaatttt tcagcggaat
    12901 ttgtgccatt aaccagttta tcccgtggtg atatcatttt ttcagcacta gattttcttc
    12961 ctctccagca gtggtagaat agcgcctcta ctcagaggtg gtgaaatgaa cgaagttgta
    13021 aattcaggcg tgatgaacat tgcgtctttg gttgtatcgg tggtggttct tcttatcggg
    13081 ctcatcttgt ggttttttat caatcgtgcc agttcacgga ctaacgaaca gattgaactg
    13141 cttgaggcgt tgctggatca gcaaaaacgt caaaatgcac tgttacgtcg tttgtgcgaa
    13201 gcaaacgaac cagagaaagc agataaaaag accattgaga gtcaaaaatc ggttgatgac
    13261 gaagatatta ttcgcctggt cgccgaacga taatccttcc tcaatcttga gtaaccccgt
    13321 cgttgaaggg ttactcaatt catcctgatt atacgcactg tcatggtgca actgcttcaa
    13381 attaaaactc tggtctgact tcaaataatt atcatattat ttatttgcgt aacgggatat
    13441 cagaaaaaga tattttcctc attaatttat taggatgttt acatcggatt tgtgattaag
    13501 cgtggtatta tttattagcg aaacgtttct ctcttgattt ttttgctcat tcatcaattt
    13561 ttcttatttt aaattttcaa tccctttggg gattgacttc tctttagggt aattgatagc
    13621 cgttaaccga ctgttttatg agaaaaagtg atataacttt ttattcattg catagcaaaa
    13681 aatgtgatat tgcacgcact atgtaataac ttctcccact ggcctggaac aactgaactt
    13741 attgaactat gttagaaaat acgccagttt aagtatctgc ctgaactggc aaggttaagc
    13801 acaatgatat atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat
    13861 cgagatttgt gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta
    13921 tgtgataaca gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt
    13981 aaagaagtcc tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt
    14041 ggtgcctcat gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca
    14101 atctaaggaa tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct
    14161 aaaggttttg gcttcattac tccggctgat ggcagcaaag acgtgttcgt acacttctcc
    14221 gctatccagg gtaatggctt caaaactctg gctgaaggtc agaacgttga gttcgaaatt
    14281 caggacggcc agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc gaatccactg
    14341 atctgaagtg tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt
    14401 actatgcttt atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac
    14461 acttgcggcc tttagtatcc tgccggagtt gtcatgtctt tttcctgtcc actttgccat
    14521 cagcctcttt cgcgtgaaaa aaacagctat atctgtcccc agcgacatca gtttgatatg
    14581 gcgaaagaag ggtatgtcaa tctgctgtcc gttcagcata aacggtctcg tgatccgggc
    14641 gacagcgcgg aaatgatgca agcacgccgc gcattcttag atgccggaca ttatcagccg
    14701 ctgcgtgatg caattgtcgc ccaactgagg gaacggcttg atgataaggc cacggcggtg
    14761 ctggatattg gctgtggtga agggtattac acacacgcat ttgccgatgc gttgcccgaa
    14821 atcaccacgt ttggtctgga tgtttcgaag gtagcgataa aagcggcggc gaaacgctat
    14881 ccgcaggtca ctttttgtgt cgcttccagc caccgtttgc cgttttccga taccagtatg
    14941 gacgccataa tacgtattta cgcgccgtgt aaagcagaag aattagcacg agtagtgaag
    15001 cccggcggct gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg
    15061 ctgatttaca atgaagtaca tcttcatgca cctcatgcag aacaactgga aggttttaca
    15121 ttacagcaga gtgcggagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca
    15181 ttattgcaga tgacgccgtt tacctggcgt gcgaagccag aagtctggca aacactggca
    15241 gcaaaagaag tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat
    15301 taaccgtgga agtgcgtcca gaggatctgg acgccgatgc cgatcagtac cagcccgccg
    15361 agaatttccg cttttttccc aataattgag ccgataaagc gaccaaccat catccctaat
    15421 gttgacataa tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctgc
    15481 aggaaagcaa gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt
    15541 accagtagcc agaaaccgtg tcgacggcgc ggctcttcat cttcatcatc tgcgccacga
    15601 aaaccctcaa taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtgg
    15661 ttccattcaa ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggcgtc
    15721 agggtttcga cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta
    15781 tggagggtgg cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca
    15841 agaagaacag tagcagtgat attcatgaca atgtcctgac cggggtatcc ataaaacaca
    15901 tcatcgcccc cagcaaacat gcatacatga acaacaagcc gtgatggttt tcgcgttcat
    15961 gcaggcatag cgattgatgt gcatatggtc tcgcctgact gaaagtattc agtccgtacg
    16021 tgccacgttt tgcaacgagt atgttgacac gtacatttcc ggaatctgga aatcggctac
    16081 tccccaaatg agggcgcaac cttaacatat tttgagaata taaaacaact gttggaatat
    16141 attatttcat tgctttattg ataacgattt tcatttggat ttatatatca atgaaacgtt
    16201 aaaataaata acaaataagg gaagaggtaa tatagcctaa gctatgtctg attattaaaa
    16261 atacactaaa catagctttg gctaaattca taacttattg agttgaaacg agaagcttat
    16321 aaattttttc cagatcgtcg atatttcgaa cgcggattaa cagccgacgc tgttctaatt
    16381 gcatcaccaa cacgccatct tccgacaagt tcatcgcttt gattcggcta tattcaatcc
    16441 agacattggc gaagaaaaaa cctttttgtt taaagatgat cttcggaacg cggatccaga
    16501 atatataaaa acccatcaga gccagtgcgc ttaataacca tgtcgttatt aacgcaccat
    16561 gattcgtgac gttgttatag ataagaatga caatcaatcc gacgaagata acgctatcga
    16621 tgcgaccacg ccggagcaaa ggaattgcca gcagggtggg gccgttacgg cggggcatga
    16681 tgaactgatc gtagatcgcg aaggccagga gtgcggcgat gaaaagaatc agtaccaggt
    16741 ccgtgattgt cattaatcct ccagataaaa aaacggggcc aaaaggcccc ggtagtgtac
    16801 aacagtctta cagtcccagc aggccgcaag cgtaaccagc gataccgatg acgaagaagc
    16861 caacgatgat ccacagcggg ttaacttttt tgcgcagtag ccacatacaa gcaaaggtca
    16921 gcagcagtgg taccaggcct ggcattaact ggtccagaat agtctggaca gtagtaacgt
    16981 gttctttgcc cgtctggtca gtaatgcgag agacaaccag cgggatgttg acatgtgtcc
    17041 acttgttaac caatgccccc atgacaaaca ggccgaggat agacgcccct tccgtcagtt
    17101 tttgcaggaa gccaccaccc atatctttaa cgatatcgat acctttggag taaccatacg
    17161 ctacgccgta gtaacgggtt gccagacgca ccaggttaaa caggatgaag aacagcagcg
    17221 gacctaacag gctgccgctc atcgcgatac cggcacccag tgctgcaaat accggacgta
    17281 cggttcccca gaagatcggg tcgcctacac cagccagcgg ccccatcaaa ccgactttga
    17341 taccgttgat agcaccgtcg tcgatctctg cgccattagc acgctgttct tccagcgcca
    17401 gggttacgcc gagaatcggc gcagccacga acggctgggt gttaaagaac tccaggtgac
    17461 ggcgaatagc ttgtttacga gcttcgttgt tctcagggta gaggcgacga attgccggta
    17521 ccatagagaa gcagaaaccc agtgcctgca tacgttcgaa gttccatgaa ccctggaaga
    17581 ggttagaacg caggaagacg ccacgaatat cactttgagt gagttttttc tcggtggtag
    17641 tttgagttgt atcaaccatt tcgctcacct gttagtccag ttcgttatcg agatcgttgt
    17701 taccagctgc ctgagcaggc gcaccggcta cgcggttgta tttcgggcta agttggatgt
    17761 agagcactgc cataacagta ccaatcacac ccagagcaac caggttaaag ttggtgaatg
    17821 ctgcggttac gaagccgagg tagaagaacg gcatcaggta gccagcacgc atcatgttga
    17881 taaccatcgc ataaccaacc accacgatca tgccaccggc gatattcaga ccattggtca
    17941 ccacttccgg aatcgcattc agcatgttct gtacttcgct ggtaccaaca gacagcgcaa
    18001 cgatgacggc cggaatagcc acacgcattg cttgcaggaa cagagaagaa acgtggatcc
    18061 aggaaatcgc tgtcaggttg ccgttatcag cagccttatc cgcagcgtgc tggaaagcaa
    18121 cggtaatagt acgaacgatg atggtcagta cctggcccgc agcggccaga gggattgcca
    18181 gtgcgatacc tgcaccaatg ctctgatgac ctgcgataac cagaatggta gaaatgatag
    18241 aagccagagc ggcgtcaggc gcaactgcag caccgatgtt catccagccc agcgcgatca
    18301 tttccagcgt accaccgata ataataccgg ttttcatatc cccaagaacg atacccacca
    18361 gggtacacgc gattagcgga cggtgaaact gaaattcatc gaggattgat cccatacctg
    18421 cgatacaggc tacgataaat accagcacaa tttgaagagt ggtaatctcc attgtacttc
    18481 tcctgtttac gtaagactta agtgtgaaaa ctgagtgata atcaacacaa tacgttactt
    18541 atcgattttg ctgatcagat ccatcatttt cagtttcgga tcggtggaaa ccttacggac
    18601 ttccagctca ataccgcgcg cattcagttt cttgaacgcc tcgatatctt tttcatcaac
    18661 cgaaaccgcg ttattcacct gggttttacc ctgacggaat gccataccac cgacgttaac
    18721 agaggtgatt ttcacgccgc cttcaacgag acgctctaca tctgttgggt tggtaaataa
    18781 cagcattacg cgttcgccag catatttcgg gttgttgtag acgcgaatca ttttggcaac
    18841 atcaactacg tgtgctgtta cgcccggagg tgcaacctgg gtgagcagtg tcttacgaac
    18901 ggtatccgca gccacttcat cactaacaac aataatacgg gagacattgg tttctttggt
    18961 ccagcgggtg gcgacctgac cgtgaatcag acggtcgtcg atacgcgcaa ggccaataac
    19021 catgtagtcg tttggcccca ttggttttgc cggagttgga gccgcttttg gtgctgcggc
    19081 agcgggtgct ggcgcggctt tttcaaccgg tttggctttc agtgctttca cgccttcacg
    19141 gcctgtttct actgccagcg caaccagttc atcaaagctt gggtcatcat cacgagccat
    19201 taacgtttcc acgagcattg gaatgttaac gcctgcaatg acttcataat gctctttgtc
    19261 gacgacaatg cggctggcag cattgaacgg gctgcctccc catgtatcaa cgagaaacag
    19321 cacgccttta gtggtgtcga gttttgccaa ctgagcgttg tacttttcaa tcagcgtttc
    19381 ggcattttca cctggaacga aatcgatcca gccgacgttt tcctgctcgc ctaacagcat
    19441 ttctgccgtt ttaagcaact gctctgcagc ccaaccatgt gtgcctataa caatagcaat
    19501 ggtcacttgc tacctccttt attatcgtta acacctcaac gtgccagatg tatttttgaa
    19561 tcgtcgtcca caatcgaatc gattcagata agggctacaa ccaaaaaccc ctgcgtttcg
    19621 cgatttattt tagatatcga aaaaattatt ttatgtgatg aagatccgta atttaacttt
    19681 cgattagcag aaatttcgaa aggtaaaata tccttgtcac attcgtttgc aaaggaaggt
    19741 aaatctttgc caaatcagag gcgtctctga tatgtttaac tcccgtttaa caaccatgga
    19801 gtatagggca gtagcccgca gtatggatcg tcaccgacgt catttcagca tcaggccttt
    19861 taacgcctgc ctttctggca ctctatgccg cacctttcgt ttgcattttg tcgttacgcc
    19921 tgcattattt ctggcgtcga atagctattc cttaagcagg agcttgtcat ggaattctta
    19981 atggacccct caatttgggc ggggctactc acgcttgttg ttctcgaaat tgtgctgggt
    20041 atcgataacc tggtcttcat cgccattctt gctgacaaac tgccgccaaa acaacgcgat
    20101 aaagcgcgtt tgctggggtt atcactggcg ctgattatgc gtctggggct gctgtcgctg
    20161 atttcatgga tggtcacgct gaccaaaccg ctatttaccg tcatggattt ctccttctcc
    20221 ggacgcgacc tgattatgtt gttcgggggg atattcttgc tgttcaaagc aacaaccgaa
    20281 ctgcatgaac ggctggaaaa ccgcgatcat gattccggcc acggtaaagg ctacgccagt
    20341 ttctgggtgg tcgtcacaca gattgtcatc cttgacgccg tcttctcgtt ggatgcggta
    20401 attactgcag tagggatggt taaccatctg ccggtgatga tggcggcggt agtgattgcg
    20461 atggcggtta tgttgctggc atccaaaccg ctgacgcgat tcgttaacca gcaccccacg
    20521 gtggtggtgc tctgtctgag cttcctgtta atgattggtc tgagtctggt ggcagaaggt
    20581 ttcggtttcc acattccgaa aggttacctg tatgccgcga ttggcttctc gatcatcatc
    20641 gaagtgttta accagattgc gcgtcgcaac tttattcgcc accagtcgac tttgccgctg
    20701 cgagcgcgta ctgccgatgc catcctgcgt ttgatgggcg ggaaacgtca ggccaatgtt
    20761 cagcacgatg ccgataaccc gatgccgatc ccggaaggtg catttgccga agaagaacgt
    20821 tacatgatta acggcgtact gacgcttgcg tcgcgttctc tgcgcgggat catgacgccg
    20881 cgcggtgaaa taagctgggt tgacgctaat ctcggggtcg atgaaatccg cgagcaactg
    20941 ctctcttcac cgcacagtct gttcccggta tgtcgcggtg aactggatga aatcatcggt
    21001 attgtacgtg ctaaagaact gctggtggcg ctggaagagg gcgttgatgt ggcggcgatt
    21061 gcttcggcgt ctccggcgat tatcgtcccg gaaaccctcg atccgatcaa cctgttgggc
    21121 gtgctgcgtc gtgctcgcgg gagctttgtt atcgtgacca acgagtttgg tgtggtacaa
    21181 ggtctggtca cgccgctgga tgtgctggaa gccattgcgg gtgaattccc ggacgctgac
    21241 gaaacgccgg aaatcattac tgatggtgac ggctggctgg taaaaggcgg tacagatttg
    21301 catgccttgc agcaggcgct tgatgttgag caccttgccg atgacgatga tatcgcgacg
    21361 gtcgcgggcc tcgtgatctc ggcaaatggt cacattcccc gtgtgggcga tgtgattgat
    21421 gtagggccac tgcatatcac catcattgaa gccaatgatt atcgtgttga tctggttcgc
    21481 attgttaaag agcaaccggc gcacgatgaa gatgagtaag cattaacgta acggcataat
    21541 gggcgtgata tgtccattat gccgggcggg cggcggttgg ctgcccgcca gccatttggg
    21601 aaaatcacgt agcggcatcg gctgtgcgta caaataccct tgcaaaatat gcacgccatg
    21661 acggcgcaaa tacttcgcct gttcttgcgt ttccacgccc tccgccacca gttcaatatt
    21721 cagtctttgc cccagcgcga tgatgatatc ggtcaccgtc gagttaaccg cgtcagaacc
    21781 tatagctgcg gtaaatgact tatcaatttt cagcatgtca ggacgtaatg tttcaagcca
    21841 ggaaaatgaa ctgttgccgg tgccgaagtc atcaatcgcc agtttgacgt ttttacgatg
    21901 tagctcgcgg gcaatccgat aatcaacatc cagtaaggca tcgcgttcgg tgatttcgag
    21961 gatcagttgc tgaatcgggt gagcgctaaa ccagtactga ttgagatctt ttatcagcac
    22021 accacggcga aaatggctgg gtgcgacgtt aatgccaaca tgaaactgac tactcatcgg
    22081 gaaaacatgg cgctgacgaa tggtttctgc catcacatag cgggtcagtg gcacaattaa
    22141 atgatgttct tccgcgatag gaataaacac atccggtgaa atccagccct gacgcggatt
    22201 gttccagcgc agcaaaatct ctacaccaat acattgctgg ctgcgcgcat taagcaaagg
    22261 ctgacagaac aattcgaact catgttgcgc caggcccaga ttgatttcgc gggaaaagct
    22321 catccggtaa gcggtcgcca gccaggcgat gtagcccacc agtaaactta gcagcaccgc
    22381 cagcggtaat tgtgttggaa gatagtgcca tgccagcgcc gtagcccccg gaccattaac
    22441 gttaatggta aaaggaaagc gttgcgaaga aacctgatag ttttcattgt cttcaggttg
    22501 cggaagggaa tctaccagcc cattaccata gagcaaatgc cgtttgtcca ccgtcaggct
    22561 ggcgctactg atttgcggca gttgtggctc aaggagcatt gccgtcagta agtcgatgtt
    22621 aatcatctcc atgaccccag cattgctact gcccgctgca ggcgtccatt gaatcaaaac
    22681 cggactgcct ttaatcaggg cacggtcgat cgtcaggcgt aaaagtggtt gcggtgcagg
    22741 aagttcagcc agaatgtcca cgacgggcac attgcgataa ccaaaaatgc tggagcaata
    22801 gagtgttccg tcttgcacca ggccaatgga tcgcacagtt tggagttttg ccgcctgttt
    22861 acgtaatggc aaatgggcga cagagcaggg cagaccaatc agcggaagca agacttcgct
    22921 acctgcctgt aggggaagca gtactttatc taattcctct acagcgtgat tggcgaattg
    22981 tactacccgt tgttgattta agttacgctg tgaaataaat cgcacgctca gggtcgaagc
    23041 gagcgtaacg agtgcgcaaa tcgtacaaac aatcattcga ttacggcgat aggttttaat
    23101 gatccgttgt gctttttgca tgcgggccac cctgtaagcg tatggcgaca aacgcctgac
    23161 gcaacagtgg aagtgtagtg gggaatatta cagcagacga gaaagcgggt ataaattcgc
    23221 ccatccgttg cagatgggcg agtaagaagt attagtcaca ctggactttg attgccagac
    23281 caccgcgtga ggtttcgcgg tatttggcgt tcatgtcctt accagtttcg tacatcgttt
    23341 cgatgacctt atccagcgag acgcgcggtg cactggtgcg gcgcagagcc atccgtgcgg
    23401 cgttaatcgc cttcacagag gcaatggcat tacgctcaat gcacggcacc tgaacctgcc
    23461 ctgcaaccgg gtcgcaggtt aaaccaaggt tgtgttccat gccaatttcc gccgccacgc
    23521 aaacctgttc cgggctaccg cccagcagtt ctgcaagacc cgcagcagcc attgaacagg
    23581 caacacccac ttcgccctgg caaccaactt ccgcaccgga aatagaggcg ttcattttat
    23641 acaatgcacc aatcgcgccc gctgccataa agtaacgggt atagatgtcc gggctgaccg
    23701 attcaataaa gtggtcatag taagccagca ctgccggaac gataccgcag gcaccgttgg
    23761 ttggcgcagt taccacacga ccaccggcgg cgttttcttc gttaactgcc agcgcaaaca
    23821 tgtttaccca gtcaatgaca ttcatcggat cgttagacag tttatcgctg gaaactaaca
    23881 tccggcgcag ggcagacgca cgacgtggca cgcgcagcgg gcctggcagt acaccttcgg
    23941 tgttcatccc gcgatcgata catgcctgca tggtttgcca gacatgcgcg aaatactcgt
    24001 cgatctcttt cttgctgtgc agcgccagtt cgttctgcat agcgagacca gacagcgaat
    24061 agccggtttc attacagtac gcgagcagtt cggtggcaga tttgaacgga tacggcacgc
    24121 tcacttcgtt ggcagcatcc tgaccaaagt gttcttcatc gacgataaaa ccgccgccga
    24181 tggaataata agttttgctg tagacgactt catcgccgtt ataggcgtgg atttgcatac
    24241 cgttttcatg cagcggcagg ttgccgttat gaaaacgcat cccgttgtcg cgcgggaaat
    24301 ccacttcatg ccgtccctgt gccagcagca gacgttcgcg ctcttctacg tcgcgaataa
    24361 aaccgggaat actgtcgata tccacggtgg caggttcgtt acctgcaaga cccataataa
    24421 tggcgatatc ggtgtggtgg cctttacccg tcagcgacag tgaaccataa acgtccacgg
    24481 caacgcgagt aacgctatcc agtaagcctt tttcgaccag atcatcgacg aactgtttac
    24541 ctgccttcat aggccctacg gtatgggaag atgagggacc aatccccacc ttaaacatgt
    24601 cgaatagact aatcacgata atactcctga caaggtgact ggacttccag taacgaacta
    24661 ataacagcgc atagtgtaag agggaacgcg gcgactggct taactattca catgaattaa
    24721 actaatcaaa aaccgcgatt taactaatat tgcgatgatt gtctcaagta gtgttttgta
    24781 gatgtaaaga taagtatagt cagggtttca caccaatttg cagcgccagc tcacgaatta
    24841 tgcctgcggt cattccccat acaaaatact gttcgtacca ggacagccat acccgatgtg
    24901 aatcgccacg gcggtagata tctaaagggt gataacgacc cagatgtaat gcctgggcga
    24961 gcggcatttc aaacaccgcc gagacttcat cttcactggc gcgatacggc agatcgggcg
    25021 ggataatgcc gaccactggg gttacctgat agccagttac gctatcgacg ggcggcagca
    25081 cgccgataac ttcaacggcg gaaggcggta tagcgacctc ttcttcagct tcgcgcagcg
    25141 cggcggcgat aactgatgcg tccgtgtcat cgactgcacc tccagggaat gccacttgtc
    25201 cagcgtgttt acgcagatga attgaacgct gagtcagcaa caaccccggt tgcggtcgac
    25261 ggacgatggg gattaacacg gcagcctgac gatgatttag ggtttcccgg ttaatttgcg
    25321 ggcgcaaaag ttgaaagcgc gataaaaaat catcaagcat caggctacgg tattccacgt
    25381 cttacttctc cagttggcgt aatatcttat taactttatc aaaagtttcc tgatattccg
    25441 cttcttcctg gctatcggcg acaattccac cgcccgcaga gcagtatatt tgtccgttaa
    25501 tggcagtcag agtgcggata gtaatgctgg tatccatgtt gccgcaaaag ctcaaatagc
    25561 caatgctgcc gcaccaggca ttacgtcgct gcggttccag ttcgtcgata atttccatag
    25621 cccgtacttt cggagccccg gttattgagc caccaggaaa agctgcgcgc agcagatcgc
    25681 tggcgtgtaa ctgttctggt agttgtgccg ttatggtgct gaccagatga tgcacggcag
    25741 ggaagggttc caccacgaac agctctggta cttttaccga acctgctacg gcaacacgac
    25801 cgatatcatt acgcattaaa tcgacaatca tcagattttc ggcacgatct ttcgctgagt
    25861 tcgccagttt ttctgcttgt ttgctatctt cctgaggatc gggcaggcgt ggtagcgtgc
    25921 ctttaatagg gcgggtctgg atttcactat tatcacaaag aataaaccgc tctggcgaaa
    25981 ggcttaaaat tgcaccctgt tcaagacgta aaaaagcgct aaatggcgcg cggttggcct
    26041 gattaagctg aaggaatgcc tgccattcat cgccagaata ggtcgcatga aaacgctggg
    26101 cgagatttac ctgatagcaa tcaccgctgt gcagatattc ctgtacctgg cgaaattttt
    26161 cgccgtactg ctcgcgggtc atattggatt gccagtcgga agtaagcgtg aaatcttcct
    26221 gcggcgagaa ttgctggctt tccagccagg cccgacgggc attgacatca ttatgactca
    26281 gcaaagaaac tgtatgacgc tggtggtcga caatgagcgc ccaatcgtag atacccactg
    26341 ccatatccgg cagaacgata tcttgttccg caatttctgg cagtgactca aaacggcggc
    26401 ccagatcgta gccaaacaac cccagtgcgc cgccctgaaa tggcaaatct tcgttatgcg
    26461 ttgggcgaat gtctgcgcga tccagcacct gctggagcac ctgtagcggg tcatcagtgg
    26521 tcgttgtgcg tttttcgctt tcactaacaa cggtttcttt acctaaagtg gttaaagtgc
    26581 aaatcggatc ggcgaccaca atatcaaagc ggctatacgg atgatcggca tagccggagt
    26641 gtaaaagcat cgcccacggc aggtggctta agcgggagaa ataaaattca gcggcgtcct
    26701 gacgccaggg taaagtaatc acagcgggag ataacgtctt catcagtcct gactctactg
    26761 gctatgtgcc gggtagcatg aaataattag cgccaacaat ttagcaggag ttaacaatgt
    26821 ttgcaggttt accttcactc acccatgaac agcagcaaaa agctgtcgag cggatccagg
    26881 aactgatggc acaagggatg agcagcggcc aggcaatcgc gttggtggca gaagagctgc
    26941 gtgccaacca ttccggcgag cgaatcgtgg cgcgttttga ggatgaagac gagtaacaca
    27001 ctaatccgta ttgatttcgt tatacaagcc gatgattatt tgattgtgag aaaaaggacg
    27061 ctttttatat gtgattaaaa gcgtcctgat ccatttatgc tgatgtaaat tttactcaga
    27121 ccagtgcttt tagttcttcc tgcatatatg ttgaaaagta ttcaggattt ttaatcacag
    27181 agcgttcacc tgactccatt ttttttgccc atccaaccat tttttccgta aacccagggt
    27241 tccagccttc ctggtcagct cgcgccgtaa tatcagcggc actcgcaggt actcccagtt
    27301 cttttaaata tttaaagatt cccttcgccg tgctttcatt catcgaactt ggtgttggcg
    27361 ctgatacatc catggcacca atgaaatcca gggctttatc tattacagca ggcatattct
    27421 tatccttaaa attaaccatg ttatcgataa ggctatatat gacctta
//
LOCUS       DAJVXP010000044        26751 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVXP010000044 DAJVXP010000000
VERSION     DAJVXP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.044"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..570
                     /locus_tag="OQS10_001805"
     CDS             <1..570
                     /locus_tag="OQS10_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292842.1"
                     /translation="LGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(613..1473)
                     /gene="rsmI"
                     /locus_tag="OQS10_001806"
     CDS             complement(613..1473)
                     /gene="rsmI"
                     /locus_tag="OQS10_001806"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS2292843.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1537..3573
                     /gene="lpoA"
                     /locus_tag="OQS10_001807"
     CDS             1537..3573
                     /gene="lpoA"
                     /locus_tag="OQS10_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS2292844.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3531..3926
                     /locus_tag="OQS10_001808"
     CDS             3531..3926
                     /locus_tag="OQS10_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS2292845.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3946..4536
                     /gene="diaA"
                     /locus_tag="OQS10_001809"
     CDS             3946..4536
                     /gene="diaA"
                     /locus_tag="OQS10_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS2292846.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4546..5121
                     /gene="yraP"
                     /locus_tag="OQS10_001810"
     CDS             4546..5121
                     /gene="yraP"
                     /locus_tag="OQS10_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS2292847.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5235..6275)
                     /locus_tag="OQS10_001811"
     CDS             complement(5235..6275)
                     /locus_tag="OQS10_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2292848.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6348..6983)
                     /locus_tag="OQS10_001812"
     CDS             complement(6348..6983)
                     /locus_tag="OQS10_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS2292849.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7111..7629
                     /gene="yhbO"
                     /locus_tag="OQS10_001813"
     CDS             7111..7629
                     /gene="yhbO"
                     /locus_tag="OQS10_001813"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS2292850.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7609..8052)
                     /locus_tag="OQS10_001814"
     CDS             complement(7609..8052)
                     /locus_tag="OQS10_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS2292851.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8103..8405
                     /gene="yhbQ"
                     /locus_tag="OQS10_001815"
     CDS             8103..8405
                     /gene="yhbQ"
                     /locus_tag="OQS10_001815"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS2292852.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8392..8895)
                     /locus_tag="OQS10_001816"
     CDS             complement(8392..8895)
                     /locus_tag="OQS10_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2292853.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8889..9413)
                     /locus_tag="OQS10_001817"
     CDS             complement(8889..9413)
                     /locus_tag="OQS10_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS2292854.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9622..10617
                     /locus_tag="OQS10_001818"
     CDS             9622..10617
                     /locus_tag="OQS10_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS2292855.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10626..11504
                     /locus_tag="OQS10_001819"
     CDS             10626..11504
                     /locus_tag="OQS10_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS2292856.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11710..12717
                     /locus_tag="OQS10_001820"
     CDS             11710..12717
                     /locus_tag="OQS10_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS2292857.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12835..14079)
                     /gene="mtr"
                     /locus_tag="OQS10_001821"
     CDS             complement(12835..14079)
                     /gene="mtr"
                     /locus_tag="OQS10_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS2292858.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14233..16122)
                     /gene="deaD"
                     /locus_tag="OQS10_001822"
     CDS             complement(14233..16122)
                     /gene="deaD"
                     /locus_tag="OQS10_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS2292859.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16115..16195)
                     /gene="yrbN"
                     /locus_tag="OQS10_001823"
     CDS             complement(16115..16195)
                     /gene="yrbN"
                     /locus_tag="OQS10_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS2292860.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16302..17186)
                     /gene="nlpI"
                     /locus_tag="OQS10_001824"
     CDS             complement(16302..17186)
                     /gene="nlpI"
                     /locus_tag="OQS10_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS2292861.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17295..19430)
                     /gene="pnp"
                     /locus_tag="OQS10_001825"
     CDS             complement(17295..19430)
                     /gene="pnp"
                     /locus_tag="OQS10_001825"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS2292862.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19677..19946)
                     /gene="rpsO"
                     /locus_tag="OQS10_001826"
     CDS             complement(19677..19946)
                     /gene="rpsO"
                     /locus_tag="OQS10_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS2292863.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20095..21039)
                     /gene="truB"
                     /locus_tag="OQS10_001827"
     CDS             complement(20095..21039)
                     /gene="truB"
                     /locus_tag="OQS10_001827"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS2292864.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21039..21440)
                     /gene="rbfA"
                     /locus_tag="OQS10_001828"
     CDS             complement(21039..21440)
                     /gene="rbfA"
                     /locus_tag="OQS10_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS2292865.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21604..24276)
                     /gene="infB"
                     /locus_tag="OQS10_001829"
     CDS             complement(21604..24276)
                     /gene="infB"
                     /locus_tag="OQS10_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS2292866.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24301..25788)
                     /gene="nusA"
                     /locus_tag="OQS10_001830"
     CDS             complement(24301..25788)
                     /gene="nusA"
                     /locus_tag="OQS10_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS2292867.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25816..26268)
                     /gene="rimP"
                     /locus_tag="OQS10_001831"
     CDS             complement(25816..26268)
                     /gene="rimP"
                     /locus_tag="OQS10_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS2292868.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26475..26551)
                     /locus_tag="OQS10_001832"
     tRNA            complement(26475..26551)
                     /locus_tag="OQS10_001832"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26515..26517),aa:Met,seq:cat)
BASE COUNT         6317 a         7419 c         6744 g         6271 t
ORIGIN      
        1 ctcggctcga tgaccattcc gaaaacggat attgccgtca ttaaaggcgc gtgggtcgat
       61 ggaatgggaa gcccgtctac aggtgacttc catgatttag tcaagttatc gattcaggga
      121 aatctcaccg ccccacagtc gtgcaaaatt aatcagggcg atgttattaa ggttaatttt
      181 ggattcatca atggtcagaa gtttaccacc cgcaatgcca tgccagacgg ttttactcca
      241 gtagactttg atatcactta tgactgtggt gatacttcaa agattaaaaa ctcgttgcaa
      301 atgcgcatcg acggtacaac tggggtagta gaccagtaca acctggtcgc caggcgaaga
      361 agttcagaca atgtgcccga tgtcggtatt cgtattgaaa atctcggcgg cggagttgca
      421 aatattcctt ttcagaacgg tatccttccc gttgatcctt ccgggcatgg caccgtcaat
      481 atgcgcgcct ggccagttaa tctggtcggt ggtgagctgg aaacaggaaa atttcagggc
      541 accgccacca ttaccgtcat ggtgcggtaa aagaagacgt tgcgttttga ttaacgcaac
      601 gcttcggcac tgttacccct gctgctccag cgcatactta tacagcgcat ttttcttcac
      661 gccatgaatt tctgcggcca gcgccgccgc ttttttcagc ggcagttctg cctgtagcag
      721 cgccagcgtg cgcagggcat cggcgggtaa gtcttcttcc tgtgctttat gaccttcgac
      781 aatcagcacc atttcgcctt tgcgacggtt ttcatcttcc tttacccacg ccagcagctc
      841 gccaacgggc gcgccgtgaa tggtttccca ggttttggtc agctcacgcg ccagaaccac
      901 gtagcgggat tcgcctaata ccgcaacgat atcttccagg ctatctaaca gacggtgggt
      961 agattcataa aaaatcagcg tgcgcggctc cgcttcaatg gcttttagcg catcacggcg
     1021 gccttttgat ttggcaggta aaaagccttc gtaacagaaa cggtcagagg gtaaacccgc
     1081 ggcgcttaac gcagtgatag cagcacacgg tccgggtagc ggcaccacac gaatccccgc
     1141 ttcacggcag gtacgcacca gatggtagcc aggatcgtta attagcggcg ttccggcatc
     1201 ggaaaccagc gcaatgtttt gcccctcttg cagcttcgcc agcagcgttt cggctttttg
     1261 ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa
     1321 taaaccggtg tgacgagtat cctcggcggc aatcagatca acggcctgta atacctctaa
     1381 cgcacgctgg gtgatatccg ccagattgcc gattggcgtc ggtacaatgt aaagctggcc
     1441 ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc cgatttaata
     1501 ttgagcattg cgtaaaaaaa tatcactgga tacattatgg taccctcaac attttctcgt
     1561 ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc tgattttcgc cggttgtggc
     1621 acccatactc ccgatcagtc cactgcttat atgcagggca cggcgcaggc tgattctgcc
     1681 ttttatcttc agcagatgca gcaaagctct gatgatacca ggatcaactg gcaattactc
     1741 gccattcgtg cactggtgaa agaaggtaaa accgggcagg cggttgagtt gtttaaccaa
     1801 ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa cactgctggc ggtagagatt
     1861 aaactggcgc agaaagattt tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc
     1921 gatttagaac aaaaccagca agcgcgttac tggcaggcaa aaatcgatgc cagccagggg
     1981 cgtccttcca ttgatttact gcgcgcgtta attgctcagg aaccgctgct cggcgcgaaa
     2041 gaaaaaaagc agaatattga tgccacctgg caggcgctct cctccatgac tcaggaacag
     2101 gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc aaggctggct ggatctgcag
     2161 cgcgtctggt ttgataaccg taacgatccc gacatgatga aagccgggat cgccgactgg
     2221 caaaaacgtt atccgaacaa tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta
     2281 aaagcgttta aaccagcctc gaccaacaaa atcgccctgc tgttgccgct gaatggccag
     2341 gcagcggtat ttggtcgcac cattcagcaa ggctttgaag cggcgaaaaa tatcggcact
     2401 cagccagtgg cggctcaggt agctgccgca cctgccgcag acgtagctga acaacctcag
     2461 ccgcaaaccg tggatggcgt tgccagcccg gcacaagcct cggttagcga tctgaccggt
     2521 gatcagcctg cggcccagcc ggtgcctgta agcgccccgg cgacaagcac cgcagcggta
     2581 agcgcacccg caaatccatc cgcagagctg aaaatctacg atacctcatc acaaccactt
     2641 agccagatct taagccaggt tcagcaggat ggcgcgagta ttgtggttgg tccgttgctg
     2701 aaaaataacg ttgaagagtt gctgaagagc aacaccccgc tgaacgtgct ggcactgaac
     2761 cagccagaga atatcgaaaa ccgcgtcaat atttgttact tcgcgctttc acctgaagac
     2821 gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta aacaagcgcc gctggtgctg
     2881 atcccacgca gttcattggg cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa
     2941 ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt
     3001 gttaacggcg gttctggtat cgctttaacg ggtaccccga ttactcccag agcgacaacc
     3061 gactccggca tgacgaccaa caatccaacg ctgcaaacca cgccaaccga tgaccagttc
     3121 accaataatg gcggtcgtgt cgatgcggtg tacattgtgg caacgccggg tgaaatcgct
     3181 tttatcaaac cgatgatcgc catgcgtaac ggtagccaga gcggtgcaat gctgtacgcc
     3241 agctcccgca gcgcacaagg caccgcaggc ccggatttcc gtctggagat ggaaggtttg
     3301 cagtacagcg aaatcccgat gctggcgggc ggtaatctgc cgttaatgca gcaggcactc
     3361 agcgcggtga ataacgatta ttcactggct cgcatgtatg cgatgggcgt cgatgcctgg
     3421 tcgctggcaa atcatttctc acaaatgcgc caggttcagg gttttgaaat caacggtaat
     3481 accggaagcc tgacggcaaa cccggattgc gtgattaaca ggaagttatc atggctacag
     3541 taccaacaag gtcaggtagt ccccgtcagt taaccaccaa acagaccggc gatgcgtggg
     3601 aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg gtttatcgcc gctaacgtga
     3661 acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg ccggaccacc gtttttgtcg
     3721 aggtacgcta tcgccgctct gcgctttatg gcggcgcggc agccagtgtg acccgcagca
     3781 aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg
     3841 atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta
     3901 aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt
     3961 aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat
     4021 gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc
     4081 tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac
     4141 cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc
     4201 ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg
     4261 ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt
     4321 gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt gaccggctat
     4381 gacggcggcg agcttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg
     4441 catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg
     4501 atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg
     4561 ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg
     4621 ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggtg
     4681 gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag
     4741 aaagaaaccc gtattaatgt cacggcgtat cagggcaaag tgctgctggt tgggcagtca
     4801 ccaaacgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac
     4861 gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc atctaacgat
     4921 acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc
     4981 aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt
     5041 gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg ggtaaccaca
     5101 gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct
     5161 tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca
     5221 tgttttccct caaatcagaa taacagcgcc agcccgccga caatcacgcc tgacactgcc
     5281 accatcgccc ccgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt
     5341 gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc ggttcccata
     5401 cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag
     5461 catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca
     5521 tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg
     5581 atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt
     5641 gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt
     5701 tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg aatcgccgca
     5761 aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg
     5821 cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat tcccgccgcg
     5881 accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt tcccagcagc
     5941 gtgccgcgaa agcgcgattg tccaagcgta cgcaacaacc agtcacgcgg gatcagcacc
     6001 tgaatcaacg aaccgaggat cacccccagc accgccgctt tccataccgc gaggaaatag
     6061 atcatcgcgt aatccaacgc cgcctgccac ggattagcat ccgcctgcgc aaggatagat
     6121 ttaccgatac tgtgggtttc ggcagcagta aacgctttgc cgtagtaagg ttgccatttc
     6181 acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg
     6241 ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa taagttatga
     6301 taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt
     6361 ttgcgtaatt ctgaagagct taaaatagtc acgccttcat gctcaggccg catcgcctct
     6421 gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc gggcagtaaa
     6481 cgaaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagcaac
     6541 atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc ttcttccatc
     6601 tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat agcactgacc
     6661 accagcatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac taccagcgtg
     6721 taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa
     6781 caaaacacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca
     6841 tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc ggctatggcg
     6901 ttaactttcg gttcgttaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg
     6961 cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc ttgcgctcca
     7021 cggctcatgc accaggctta atagaccatg aggtaattat ccccgattgt gtgaaattcg
     7081 cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcagt
     7141 gatgaatttg aggattcaga atttacttca cccgcagacg agttccgtaa agccggacac
     7201 gaagtgatta ccattgaaaa acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc
     7261 agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgctg
     7321 ctaccgggtg gtcattcacc ggattatctg cgtggcgaca accgttttgt cacctttacc
     7381 cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg
     7441 atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat
     7501 gttaaaaatg cgggcgcgga attttacgat caggaagtcg tggttgataa agatcagctg
     7561 gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc
     7621 ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa
     7681 tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg caaccggaaa
     7741 gcgacgattg tacgcctggc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc
     7801 acctttaaac tgcacgccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc
     7861 ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat
     7921 gtagaaggcg actttctgcg catcaaacag ataaaaagca ttagcgcacc acagttcccc
     7981 ttcctgctgc acacaccagg tgacaacatg ttgttttgcc aaccaacggc tgatggtgat
     8041 gagtgtttcc attgctgctc tcttttatac tgtgggcctg accttaacat gcgacgcaga
     8101 cgatgacacc ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga
     8161 tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac
     8221 tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgat cgttcgctgg
     8281 cgttacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag
     8341 cggaaggcgc agggtttgca gagctgttaa gcagtctgca aaccccggag attaaaagcg
     8401 attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt catccgctaa
     8461 acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc
     8521 cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca ccactgcggc
     8581 atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg
     8641 tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca ggtcttcgcc
     8701 ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc
     8761 caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc
     8821 atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc
     8881 tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt
     8941 cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc
     9001 tccagttcaa tggcgtccat caggtttttc acatacagcc ccagctccgt atcgccttca
     9061 atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc cgcaatcatc
     9121 agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgcgttctg gctgaccacc
     9181 agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt
     9241 aaccagcggc cttcaagaaa ctccagctca ccatcatcca gcgcctgacg gaattgccag
     9301 cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca
     9361 ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa cacgagtttt
     9421 actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa
     9481 atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca
     9541 aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgaaa
     9601 gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt
     9661 aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgatactaat
     9721 gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt
     9781 gtccatcaac atcgccgcaa acttcacatc gcgattaaca cttttgcgca tccggacggt
     9841 tacgcccgtt ggcagcgcgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc
     9901 ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt acccgcatat tgagcgtcat
     9961 gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt
    10021 gatgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca
    10081 cgggtcacac ctgtcccgct ggaagtcttt gcttttggca gcctgtgcat tatgtcggaa
    10141 ggtcgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacaccgt gggcgcgtgt
    10201 tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac
    10261 gaagtgctga tcgaccgtta tcaggatggc gaaaacgcag gttatccgac gctgtgtaaa
    10321 gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat
    10381 accctggaac tgctgccgga gttaatggcg gcgaatattg cttcggtgaa aattgaaggc
    10441 cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca ggctatcgac
    10501 cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg
    10561 tcgatgtccg aaggcacgca gaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa
    10621 gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag gcgctggaag
    10681 aattttatca gcaggccgcc accagcagcg ccgacgtgat ttatcttggt gaagcggtat
    10741 gcagcaagcg ccgggcaacc aaagttggcg actggctgga gatggcaaaa tcgctcgccg
    10801 gaagcgggaa gcagatagtg ctctccacgc tggcgctggt gcaggcatcc tctgaactgg
    10861 gcgaactgaa acgctatgtt gagaacggtg agtttctgat tgaagccagc gatctcggcg
    10921 tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct
    10981 acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg tgtatgccgg
    11041 tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag ttggggattc
    11101 gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc tactccgccc
    11161 gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta
    11221 agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg
    11281 gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctgaca tccatgcagg
    11341 gattggttga tgtggtccgc ctgtcaccgc agggtactga cactttcgcg atgctcgacg
    11401 ccttccgcgc taatgaaaat ggcgctgcgc cactgccgtt gacggcaaac agcgactgta
    11461 acggctactg gcggcggctg gctgggctgg agctgcaagc ctaagtaaat atgctcgtca
    11521 tacttcaagt tgcatgtgct gcatctgcat tcgttcaccc cagtcactta cttatgtaag
    11581 ctcctgggga ttcactctct tgtcgccttc ctgcaactcg aattatttag agcacagctc
    11641 actttgttaa caactttaac tactctttaa tgcagtatta aagattaatc ggtaacaaag
    11701 tgagctgtta tgactgataa aaccattgcg ttttcgctac tcgatctggc ccccattccc
    11761 gaaggttctt cagcgcgaga agcattctcc cactctctcg atctcgcccg tctggctgaa
    11821 aagcgcggct atcatcgcta ctggctggca gaacaccaca atatgactgg cattgccagt
    11881 gctgccacgt cggtattgat tggctatctg gcggcgaata ccaccacgct gcatctgggg
    11941 tctggcggcg tgatgttgcc taaccactca ccgttggtca ttgccgaaca gttcggcacg
    12001 ctcaatacac tctatccggg gcgaatcgat ttggggctgg gtcgtgcgcc aggtagtgac
    12061 caacggacaa tgatggcgct gcgtcgccat atgagcggcg atattgataa tttcccccgc
    12121 gatgtcgcgg agctggtgga ctggtttgac gcccgcgatc ccaatccgca tgtgcgcccg
    12181 gtaccaggct atggcgagaa aatccccgtg tggttgttag gctccagcct ttacagcgcg
    12241 caactggcgg cgcagcttgg tctgccgttc gcgtttgcct cacacttcgc gccggatatg
    12301 ttgttccagg cgctgcatct ttatcgcagc aacttcaaac cgtcggcacg actggaaaaa
    12361 ccatacgcga tggtgtgcat caatattatc gccgccgaca gcaaccgcga cgctgaattt
    12421 ctatttacct caatgcagca agcctttgtg aagctgcgcc gtggcgaaac cggacaactg
    12481 ccgccgccga ttcaaaatat ggatcagttc tggtcaccgt ctgagcagta tggcgtgcag
    12541 caggcgctga gtatgtcgtt ggtgggtgat aaagcgaaag tgcgtcacgg cttgcagtcg
    12601 atcctgcgcg aaaccgacgc cgatgaaatt atggttaacg ggcagatttt cgaccaccag
    12661 gcgcgcctgc attcgtttga actggcgatg gatgtgaagg aagagttgtt gggatagtgt
    12721 gtcttaacgc gggaagcctt atccgagctg gcaacgctgt cctacgtaga cctgataagc
    12781 gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg ctgattactg
    12841 atacaccggc agcaaattaa agctcgataa gatatggacc agtgcgttgc caacgccaaa
    12901 caccagaatc agcgtaatca tcggcttgcc gccccagaca cggaatttcg ggctgccaaa
    12961 gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga tggtagctgc
    13021 taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac cacccacaac
    13081 aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt catcgaaacc
    13141 aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg aactcgctac
    13201 cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca gcacgccgct
    13261 taatgcctgt accagcacat caatattacc gcccttctct gcaataccga taaactccgg
    13321 acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca gtgccatcag
    13381 cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat acttcatcag
    13441 gctcggcacg ttaccgtgat aaccaaacga tgccagacag aacggcaggg tcatcagcaa
    13501 atacggcgca taagatgcat tgctttcggc gacgttgaac aatgtcgcag gctgcacatg
    13561 ccccagtagg ctaccgaagg tgaggaagaa ggtaatgact ttcgccccca gtacaatcgc
    13621 cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca gcagtgcaaa
    13681 accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg tgtgatgcag
    13741 aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa aggcaatgga
    13801 aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg tgtcaaaact
    13861 cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat gcagcataca
    13921 gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg acatgaccac
    13981 tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca cgccgccaag
    14041 cagcgacggt gacgtttggg tggtggttag tgttgccatg agagcttctc tccagtgaaa
    14101 aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac aaaagacaga
    14161 aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat tgtgttcgct
    14221 gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt catcacgacg
    14281 cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac ggccaccttc
    14341 acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga aaccacgacc
    14401 accgccacga cgctcaccgc cagtatgcgg ctgtgcatcg cccagtaact gcatgttcat
    14461 cggcttgttg agaatgcgag tgcgcgtaaa gtgttgcagc acttcacccg gcataccttt
    14521 cggcagttcg atggtggagt gagaagcaaa cagcttgatg ttaccaatgt aacggctact
    14581 gatgtcgcct tcgttagcaa tcgcaccaac gatatgacga acttcaacac catcatcgcg
    14641 gcccacttca atgcggtaca gctgcatatc gccaacatca cgacgttcac gacgcggacg
    14701 atcttcacgg tcaccacgcg ggccacggtc gttacgatcg cgcggaccac ggtcatcacg
    14761 gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggtacgatca gagtacgttc
    14821 accctgtgcc attttcagca gtgccgcagc cagagtttcg agatccagct cttcaccttc
    14881 tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc tgctttccag
    14941 ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca gttctgcgtt
    15001 cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc gcagcagacg
    15061 acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac gaccggtacg
    15121 accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa ctaccaggct
    15181 gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga tgtccagacg
    15241 accatctttc aggcgttcca gtgtctgttc acgcagcgcc tggttcatgt caccgttcag
    15301 cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag tcgcgttttt
    15361 ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac gtaccagtgc
    15421 ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag gacgggtagt
    15481 cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg taatgcgacg
    15541 aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg ggatctgcgc
    15601 cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag cttcatccag
    15661 aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt ccagcagacg
    15721 gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt gcacgtcata
    15781 acgctggccg ccgtacagag caaccacatt tacgccgcgc atgtgtttag agaaatccgt
    15841 cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca gaatctgtgg
    15901 tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg cagtttttcc
    15961 gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg gaatacactc
    16021 tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa ggataggagc
    16081 cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg tgtgcctcaa
    16141 aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt ttcattgaaa
    16201 agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac ccgttaaggt
    16261 gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg gtccgattct
    16321 gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta tcggtgctca
    16381 acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc gctgtccaaa
    16441 tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag atgctcagcg
    16501 agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg ttcgctaatg
    16561 ttgcccaagt agaactcgac aatgttccat ccccactgtt ccttatccga tttttcgaag
    16621 tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc gagataaagc
    16681 cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag atcatcttgc
    16741 gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg cgcgtagttg
    16801 taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc aaaattgcct
    16861 gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg tcggattgcc
    16921 agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc atacaacact
    16981 ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact ggcaaggatt
    17041 tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg taccgcgagg
    17101 acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc tgtcgcaacg
    17161 aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt gaatgaacgt
    17221 cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc cgcagcggag
    17281 ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc tgcagcaggt
    17341 tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg atcaacttct
    17401 agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt ctcaacgcgt
    17461 ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat ggcaacaaat
    17521 gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac ttcgatttct
    17581 gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc ggtcgctgcg
    17641 atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc ggtcagggca
    17701 cggataacag agccgccttt accgataacg tctttgatct tatccgggtt gatcttgatg
    17761 gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat cgcctgttcc
    17821 attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac ctgcatgatc
    17881 tctttggtga taccttcaat tttgatatcc atctgcagtg cagagatacc gtcgcgggaa
    17941 cctgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat gtcagacagt
    18001 acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac ggcagctttg
    18061 attggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc catagaagaa
    18121 gaaccgttgg attcggtgat ttcagacact acacgtacgg tgtacgggaa tttgtccata
    18181 tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat ttcacgacgc
    18241 ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt gtagtggaac
    18301 aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc acgagcggta
    18361 cccagcgttg cagtaaccag tgcctgcgtt tcaccgcggg tgaacagcgc agaaccgtga
    18421 gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt ttcacgaccg
    18481 tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc gatagcgtgc
    18541 agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag cgtcgcgatg
    18601 gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt gatgcggtaa
    18661 gcatcactca gacgagcttc agccagtgca gcaacgcgcg cgttcagcgc ttcgtttacc
    18721 ggctccggct gccagtccca acgcggttta ccggcttctt tcactagttc attgatgttc
    18781 tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat ctgatcttcg
    18841 ctcagcagtt cagcttcaga ttcaaccatc agtacagcgg cttcagtacc ggcaacaacc
    18901 agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta ctggtcattg
    18961 atgtaaccta cgcgggcagc accaatcggg ccattgaacg gaataccaga cagagacagt
    19021 gctgcggaag caccaatcat cgcgacgata tccgggttaa cctgcgggtt aacagaaacc
    19081 acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg gcgaatcggg
    19141 cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg gacgaccttc acgacggaag
    19201 aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac ggtcagtggg
    19261 aagaagtcct gacctggttt ggcttttttc tgtccaacaa cggtaacgaa taccgcggtg
    19321 tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc ggtttccaga
    19381 gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa tgtaatatcc
    19441 tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct tctgcgcatc
    19501 ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc attagccgcg
    19561 cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca gtgaattgct
    19621 gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg caagaattag
    19681 cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt acgtttcagg
    19741 tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg gctgtggtga
    19801 tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt cagcagtgct
    19861 acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga aacgatttta
    19921 gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa tgaaaggacg
    19981 ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca atttacgcga
    20041 cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt aacattacgc
    20101 cgggtattca accaccaggc gacgaggcgc gacgcggcct tcatcgtcaa tttcgcccat
    20161 accgataaac ttgccgttct caccttctgt aacgcgaacc agtccttcca gcggcgcacc
    20221 ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg gaagattcac
    20281 caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa gtaactccgc
    20341 agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt ccagggtcac
    20401 catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa catgcgcgcc
    20461 acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc cttttgagca
    20521 gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt cataaacggt
    20581 aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt acagtttttt
    20641 accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc cacggaaagt
    20701 atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt caacgatttg
    20761 tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc gatagcgttt
    20821 gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa ttggcaacat
    20881 gcctgttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt tataaatccg
    20941 tttcactttt tgcagcgcat cattgctgga cataccctgc ggtttatcca gcaacaaaac
    21001 gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt gctgtcgtcc
    21061 gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt tgacatgcgc
    21121 atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat acgcaggcgc
    21181 atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc tttgatgccc
    21241 gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt ggcatacgcc
    21301 aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg aggatcttta
    21361 atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac gcgctgcggg
    21421 cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt tagccccttt
    21481 ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg cgccagcgtc
    21541 acatcaggca atccatgccg gatgcggcgt aaacgcctta tcccgcatgg aaccctaaaa
    21601 accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca catcgccagt
    21661 gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac ggacttcgtt
    21721 aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa ccacgttgtc
    21781 acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca tacagcctgc
    21841 gatagcacca aatttcggcg atttgaacac gtcacgaact tccgccagac cgataatctg
    21901 ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt caatcaggtt
    21961 atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt tacgtgcaga
    22021 ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag ccagggtggc
    22081 gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa cttcgtcagt
    22141 agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt ctgccttcag
    22201 gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt tctcgagttt
    22261 agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat acagtgcaac
    22321 ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag ccggtacgcc
    22381 ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt cctgacccag
    22441 ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga tatcgccctt
    22501 gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt tatcgaggaa
    22561 ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct ccagaacttc
    22621 cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt tcgcagatac
    22681 gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact gggagagttc
    22741 gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg caaccaccac
    22801 cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca tcacgccgtc
    22861 gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg cacgcattga
    22921 agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt cagtttcaac
    22981 gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa ctttcgttga
    23041 acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga tggtcacaac
    23101 cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg cctcttccag
    23161 ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca gctgtgcggt
    23221 ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca tcgctttgat
    23281 gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga tggtttcgcc
    23341 gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct gcagggaaga
    23401 acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt cacgatcagc
    23461 ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt tacgaccacg
    23521 gccacgaccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag catgttgaga
    23581 agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag tccatttgtt
    23641 ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt cgagtttacg
    23701 atgcgcttct tcttcggctt tacgcttgag ctctgcagct tcctgctcac ggcgggcttt
    23761 ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt ctttttccgc
    23821 agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcag cctcacgttc
    23881 agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg cttgctcttc
    23941 cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt
    24001 cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc caccggtacc
    24061 aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt
    24121 tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag agtcgtcagc
    24181 agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg
    24241 ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg ctacagttta
    24301 ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg ctccggcctt
    24361 ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg cgagatcttc
    24421 cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta ccccttcaag
    24481 gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg cctgtgcaat
    24541 ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct catcaaggcc
    24601 ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg tcgagaagcc
    24661 ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc
    24721 gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg tcatcacgtt
    24781 gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac ggccaatcgc
    24841 ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt catccaccac
    24901 gatagaagca acgtctgccg gtgccattgc gttaatcacg aactgcgccg ggttatcatc
    24961 ccacaggacg atatcgatac gctcgccacc cagttcagta gacaccgcct gaacacgcgc
    25021 gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc
    25081 gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca cttcttcgcc
    25141 gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg aacgagtgac
    25201 gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac gaacgcggtc
    25261 gccagggcgg aagttttcac gcggcagcat atcttcacgc aggatcacgg cttcagcgtt
    25321 gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat
    25381 ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt cacgcacttt
    25441 ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa cagactcaat
    25501 ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg cggcttcaag
    25561 ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac ggaaggtgtc
    25621 aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt catatttttt
    25681 ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc
    25741 cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg cgggcttttc
    25801 acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat tactcagcgc
    25861 gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat
    25921 aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag tcacctcttc
    25981 tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac ccggtgagga
    26041 gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag cacttacctg
    26101 gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa tatagatgcg
    26161 cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac ccagggcctc
    26221 aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc ccacccccaa
    26281 gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa aaaccccgat
    26341 aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga gcactttcca
    26401 gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat ttgaaaaagg
    26461 actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc
    26521 ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc tactcgtttt
    26581 gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt aaaatctgca
    26641 ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcat gtgaatgaat
    26701 atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctccccc t
//
LOCUS       DAJVXP010000045        26628 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVXP010000045 DAJVXP010000000
VERSION     DAJVXP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.045"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            110..319
                     /gene="asr"
                     /locus_tag="OQS10_001833"
     CDS             110..319
                     /gene="asr"
                     /locus_tag="OQS10_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HCS2292869.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            412..495
                     /locus_tag="OQS10_001834"
     CDS             412..495
                     /locus_tag="OQS10_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292870.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            595..1416
                     /locus_tag="OQS10_001835"
     CDS             595..1416
                     /locus_tag="OQS10_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS2292871.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1455..1784)
                     /gene="mdtI"
                     /locus_tag="OQS10_001836"
     CDS             complement(1455..1784)
                     /gene="mdtI"
                     /locus_tag="OQS10_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS2292872.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1771..2136)
                     /gene="mdtJ"
                     /locus_tag="OQS10_001837"
     CDS             complement(1771..2136)
                     /gene="mdtJ"
                     /locus_tag="OQS10_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS2292873.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2548..3582
                     /gene="tqsA"
                     /locus_tag="OQS10_001838"
     CDS             2548..3582
                     /gene="tqsA"
                     /locus_tag="OQS10_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS2292874.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3607..4995)
                     /gene="pntB"
                     /locus_tag="OQS10_001839"
     CDS             complement(3607..4995)
                     /gene="pntB"
                     /locus_tag="OQS10_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS2292875.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5006..6538)
                     /gene="pntA"
                     /locus_tag="OQS10_001840"
     CDS             complement(5006..6538)
                     /gene="pntA"
                     /locus_tag="OQS10_001840"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS2292876.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7062..8006
                     /locus_tag="OQS10_001841"
     CDS             7062..8006
                     /locus_tag="OQS10_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2292877.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8192..9574
                     /locus_tag="OQS10_001842"
     CDS             8192..9574
                     /locus_tag="OQS10_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2292878.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9611..10333
                     /gene="folM"
                     /locus_tag="OQS10_001843"
     CDS             9611..10333
                     /gene="folM"
                     /locus_tag="OQS10_001843"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS2292879.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10330..10665)
                     /locus_tag="OQS10_001844"
     CDS             complement(10330..10665)
                     /locus_tag="OQS10_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS2292880.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10794..11513
                     /gene="rstA"
                     /locus_tag="OQS10_001845"
     CDS             10794..11513
                     /gene="rstA"
                     /locus_tag="OQS10_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS2292881.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11517..12818
                     /gene="rstB"
                     /locus_tag="OQS10_001846"
     CDS             11517..12818
                     /gene="rstB"
                     /locus_tag="OQS10_001846"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS2292882.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12894..13823
                     /gene="tus"
                     /locus_tag="OQS10_001847"
     CDS             12894..13823
                     /gene="tus"
                     /locus_tag="OQS10_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS2292883.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13820..15223)
                     /gene="fumC"
                     /locus_tag="OQS10_001848"
     CDS             complement(13820..15223)
                     /gene="fumC"
                     /locus_tag="OQS10_001848"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS2292884.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15366..17012)
                     /gene="fumB"
                     /locus_tag="OQS10_001849"
     CDS             complement(15366..17012)
                     /gene="fumB"
                     /locus_tag="OQS10_001849"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS2292885.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17211..18386
                     /gene="manA"
                     /locus_tag="OQS10_001850"
     CDS             17211..18386
                     /gene="manA"
                     /locus_tag="OQS10_001850"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS2292886.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18487..19995
                     /locus_tag="OQS10_001851"
     CDS             18487..19995
                     /locus_tag="OQS10_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS2292887.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20176..21486)
                     /gene="uidC"
                     /locus_tag="OQS10_001852"
     CDS             complement(20176..21486)
                     /gene="uidC"
                     /locus_tag="OQS10_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS2292888.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21525..22898)
                     /gene="uidB"
                     /locus_tag="OQS10_001853"
     CDS             complement(21525..22898)
                     /gene="uidB"
                     /locus_tag="OQS10_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS2292889.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22895..24706)
                     /gene="uidA"
                     /locus_tag="OQS10_001854"
     CDS             complement(22895..24706)
                     /gene="uidA"
                     /locus_tag="OQS10_001854"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS2292890.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25095..25685)
                     /gene="uidR"
                     /locus_tag="OQS10_001855"
     CDS             complement(25095..25685)
                     /gene="uidR"
                     /locus_tag="OQS10_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS2292891.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25906..>26628)
                     /gene="hdhA"
                     /locus_tag="OQS10_001856"
     CDS             complement(25906..>26628)
                     /gene="hdhA"
                     /locus_tag="OQS10_001856"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS2292892.1"
                     /translation="ITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVDEIQQLG
                     GQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADFRRAYEL
                     NVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAF
                     DLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCS
                     PAASWVSGQILTVSGGGVQELN"
BASE COUNT         6675 a         6902 c         6390 g         6661 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggca gcgaagaaac acgccgggaa acacagccat cagcaaccgg caaaacctgc
      301 tgcacaaccc gcagcgtaag ttttcaacag taatgctggc gcgccccctc gcgcctgaaa
      361 attacggtgc taagcgggta acgtttagca ccgcctttag cggagggtag tatggtgggc
      421 cgttatcgct ttgagttcat tcttatcatc cttattttat gcgcactgat tgccgcccgt
      481 ttttatcttt cctgactgta gttatctgat tttactccca ctttcatccc gtcccgtcta
      541 tagtatttat gagggtttgc ttttaataat cataattacc caccagagtg tgatatgcgt
      601 acaaccattg ctgtagtgtt gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat
      661 aaaccagacg ttgccaaatc ggcaaacgat gaggtcagca ccctgttttt tggtcatgat
      721 gatcgtgtgc cagtgaatga cacgacccaa tcaccgtggg atgcggttgg gcaactggaa
      781 acggccagcg gcaatttatg tacggcgacg ctgattgcac ccaatctggc attaacggca
      841 ggacactgtt tattgacacc tccaaagggt aaagccgata aagcagtggc gctgcgtttt
      901 gtgtcaaata aaggtctttg gcgctatgag atccacgaca tagaaggccg cgttgatccg
      961 acactgggaa agcggttaaa agcagatggg gatggttgga ttgtacctcc cgcagccgcg
     1021 ccgtgggact tcggattgat tgtgctacgt aatccccctt ctggcattac accgttgccg
     1081 ttatttgagg gagataaagc cgcgcttact gccgcattaa aatcggctgg ccgtaaagta
     1141 actcaggcgg gataccctga agatcatctc gatacgttgt acagtcatca aaactgtgaa
     1201 gtgactggct gggcgcaaac gtcggtgatg tcacatcagt gcgatacctt gccgggtgac
     1261 agcggttcgc ctctgatgtt gcataccaat gacggctggc aattaattgg ggtgcaaagt
     1321 tcggctcctg ccgcgaaaga tcgctggcgc gccgataacc gggccatttc tgttaccggt
     1381 tttcgcgaca agctggatca actgtcgcaa aaataatgtt caaacgctgc ccgactgtgc
     1441 gggcagcgtg ttcatcaggc aagtttcacc atgatcattc cagccaacag caagaccagg
     1501 ccaatccagc ctttacgatt taaccgttga ccaaacaaga tccaacctgc ggctaacgtg
     1561 gcggcaatac caaacccgcc ccacaatgca taagcgacag acaagtcgat ccctttaacg
     1621 gcctgagaaa gcgcactaaa ggcagccagc accgccgcca gggagagcaa gccaaatatt
     1681 ttgcgacgaa agccgtcaga aaatttcaaa aagacgttag caacgatttc cagcacgatt
     1741 gccaatgcca gccaggcggc gtgaacccat tcaaactgcg ccatggttca cctccagttc
     1801 aggtttacgc gctttacggg tacctgattt tatcaacaca atcccggcga ccagggtggt
     1861 taacccggca attttcatca gcgataaact ttcgtcgaat aacaaaacgc taaacaaggt
     1921 aataaataaa ataccgatac cttcccacag cgcataagct acgcctaagg cgatcttttt
     1981 aacggcgaaa gagagaaata tatacgacag agaaatcatc accagcatta aaataaagcc
     2041 gccatttccc tcactgacgc tcgcccattt cattgacagc gtaccggtaa tttctgtagc
     2101 aatagccagg cctaataaaa tccaataaat atacattgtc cttctcctgc aagagaatta
     2161 ttttaatttt cgcttaattc agcgaaacca aagtaaagtg gtcaagcccc gcgcataggg
     2221 caagcttagg cagaagaaag gactaaagcg cgttgcgcca gtgctgctca cctacgagca
     2281 agatagtgga tgaggtacga agatgggtaa atgttgaaaa taacgtcctg aacaaattgt
     2341 ccataatatt acaattatcc gcagtgttgc ttctcgtcat cgcggatgat aattgtcctc
     2401 ggtagttgaa cacgcctgat ttgtatcata gcttaagaat taactcaaaa tattttcact
     2461 tctttacctg agcggtttga ttttcgttat gatgacggag cgaaaaagac attattatta
     2521 gcaaaggaag aaaaaacggg gacaagcatg gcaaagccga tcatcacgct caatggccta
     2581 aaaatcgtca ttatgttggg aatgctggtc attattctct gcggtatccg ttttgccgcc
     2641 gagatcatcg tgccgtttat tctcgcatta tttattgctg ttattcttaa cccgctggtg
     2701 caacacatgg tccgctggcg agtgccgcgt gtactggcgg tgtcgatttt gatgaccatc
     2761 atcgtgatgg cgatggtgtt gctattagct tatctgggtt ccgcgctcaa cgagttgacg
     2821 cggacgttac cgcaatatcg caactctatt atgacgccgc tgcaagctct tgagccgttg
     2881 ttgcaacgcg tagggattga cgtctcagtt gaccagctgg cgcattacat tgatccgaac
     2941 gcggcgatga cgttgctcac caacttattg acgcagttat ctaatgccat gtcatcaata
     3001 tttttattgc tgctgacggt gctgtttatg ctgctcgaag tgccacaatt gcccggaaaa
     3061 tttcagcaaa tgatggcgcg tccggttgaa gggatggcgg cgattcaacg tgcaattgac
     3121 agtgtttctc attatctggt gctgaaaaca gccatcagca tcatcaccgg cctggtcgcc
     3181 tgggcgatgc tcgccgcact cgatgttcgc ttcgcttttg tctggggatt gctggccttt
     3241 gcgcttaatt acatccctaa tattggttca gttctcgcgg caatcccccc tatcgctcag
     3301 gtactggtgt ttaatggctt ctacgaagcg ttgctggtgc tggcgggata tctgctgatt
     3361 aatctggtct tcggcaatat tctggagccg cgcatcatgg gacgtgggct ggggctttcc
     3421 acattggtgg tatttttgtc gttgattttt tggggatggt tgttaggacc ggtgggtatg
     3481 ctgatttccg tgccgttaac aattattgtc aaaattgcgc ttgaacaaac agcgggaggt
     3541 caaagcatcg ccgttctgtt aagcgatctc aataaagagt gacggcctcc gcagaggccg
     3601 tcagggttac agagctttca ggattgcatc cacgctagct ttggcgtcac caaacagcat
     3661 gtgggtgttt tccttgaaga acagcgggtt ttgcacacca gcatagccag tgttcatcga
     3721 acgtttaaag acaatcacgt tctgcgcttt ccacacttcc agcacaggca taccagcaat
     3781 aggactcttc ggatcatcct gcgccgccgg gttaaccgta tcgttagcac caatcaccag
     3841 tacggtatcg gtatcagcaa agtcatcgtt gatctcgtcc atttccagca cgatgtcata
     3901 cggtactttt gcttcagcca gcaatacgtt catatgtcca ggcaaacgcc ccgcaaccgg
     3961 gtggataccg aaacgcacgt tgataccacg agcgcgcagt ttctcggtaa tttcagcgac
     4021 aggatattgc gcctgcgcga ctgccatgcc gtaccccgga gtaatgatca ctgaatggga
     4081 gtttttcagc agttccgctg tctcttctgc ggtgatttcg cggtgctcgc ccacttcctg
     4141 atcatcgcca gtagaagagc cgtcggtgcc gaaaccaccc gcaataacgc tgataaagga
     4201 acggttcatc gccttacaca taatgtaaga aaggatagcc cccgaagaac cgaccagcgc
     4261 accggtcaca atcagcaggt cgttgctgag cataaagccc gcagccgcag ccgcccagcc
     4321 ggagtacgag ttcagcatcg acaccaccac tggcatatct gcaccaccga tggaggcgac
     4381 taaatgccag ccgaatacca gcgcaatggc ggtcattatt agcaatgcca gcacttgcag
     4441 gccgacgctg tccgtgcgaa caaatacaat cagcagcagg aaggaaacga ccagagccgc
     4501 caggttcatt ttgtgacggt ttggcagcat caacggttta gacgaaatct tgccacacag
     4561 tttgccgaac gccaccaccg aacccgtgaa cgttaccgcc ccgatgaaga taccgaggaa
     4621 cacttccgtc aggtgaatat tgaccagaat cggtgccatt cccgcgtcat gatgcagata
     4681 gctgttaaag ccaaccagca ctgccgccag acccacgaag ctgtgcagga tcgccaccag
     4741 ttctggcatt tcggtcattt caactttctt cgccagacgg ataccaattg ccccaccaat
     4801 gaccatcgcc agcaagatcc agccaacatt acccgtatcc ggtccaaaaa tggttgcgat
     4861 taacgcaatc gccatcccgg cgataccgaa gttgttaccc tggcgagacg tttcatgttt
     4921 cgaaagaccg gccagactga agataaacag gatcgcggca acaatgtatg cagctgtaac
     4981 taatcctcca gacatatgtt accccttaat ttttgcggaa cattttcagc atgcgctgag
     5041 tcacggtgaa gccaccgaaa atattaatgc tggctataag caccgcgata aaactaagga
     5101 agctaaccca gccgccctgg ccaatctgca acagtgctcc gacaacaata atccctgaaa
     5161 tcgcgttggt gaccgacatc aacggtgtat gcagcgcgtg cgatacattc cacaccacgt
     5221 aataaccgac aacgcaggcc agcgcgaaaa cggtgaagtg cccaagaaac tctttcggcg
     5281 caacgcttgc cagccaaccg aaaaggatga ttgccagcgc catcaacgcg tatttacgcc
     5341 acggtgagca ggcacatttt tcctcagttt tcacttccgg tgccgctttt tgtgccgcct
     5401 gcggctgagc tgatacctga atcggcggtg ccggccaggt aatttcgccc gcacggatca
     5461 cggtcacgcc gcgaatcacc acatcatcaa aatcaacagt gatattgccg tctttctctt
     5521 tgcacaacag tttcagcaga ttaacgaggt tagtaccgta aagctgcgag gattgcgtcg
     5581 gcagacggcc cggaagatcg gtataaccaa tcactttgac accattttcc gtagtgaaga
     5641 tttcacccgg cacggtgtat tcacagttgc caccgttttg ggctgccagg tcgacaatca
     5701 cactgcccgc cttcatggag tcaaccattt cacgggtaat tagcttcggc gctggtttgc
     5761 ctggaataag cgcggtggtg acaatgatat cgacctcttt tgcctgggcg gcaaagagtt
     5821 ccatttccgc tttaatgaac gcgtccgaca tcactttggc atagccatcg ccgctgcccg
     5881 cttcctcttt aaaatccagc tcgaggaatt ccgcgcccat actttgaact tgttctttca
     5941 cttccggacg ggtgtcgaat gcacgcacaa tcgcgccgag actgtttgct gcgccaatgg
     6001 cggccagacc tgcaacaccc gcaccaatca ccatcacttt tgccggtggc actttcccgg
     6061 ccgcagtaat ttgcccggta aagaagcgcc caaattcatg tgccgcttca acaatggcgc
     6121 gataaccggc gatgttcgcc atcgagctta gtgcgtccag cgattgtgcg cgtgagatac
     6181 gcggcacaga atccatcgcc atcacggtca cgttacgttc cgcaagtttt tgcattaatt
     6241 ccggattctg cgcaggccag ataaaactca ccagcgttgt cccaggattc agtaacgcaa
     6301 tttcatcatc taacggcgca ttgaccttca gaatgatctc tgactgccag acgctattcc
     6361 cttctacaat ttcagcgccc gcttgcacaa acgctttatc gtcaaaactt gccagttgac
     6421 ccgcgccgct ctctaccgcg acggtaaaac ccagtttcag cagctgttcc actgtttttg
     6481 gcgttgctgc aacacgggtt tcattggtta accgttctct tggtatgcca attcgcatga
     6541 tattcccttc catcggtttt attgatgatg gtttgcctgt gtcaggagcc acacaagccg
     6601 ctcatgtacg agctaaatgt tactccgtta aaataaatta gtaacaaacg ccctataacg
     6661 tactgaaaat tatgcctgtg atctagcgcc aaaaatcagt atttcggcgt gaactcgcaa
     6721 aatattaacg attcagccgt gatagtggga taaacacctt agaacgccgg ataaagactg
     6781 ataattgtct tcgacggccg ggtaaaacga gacatcgccc cggcacgatt cactacttaa
     6841 cattaaatta acttatacaa ttcagttgct tcagtagtaa tgatgctgat acggctgttt
     6901 tttaagcata gacggtcatt tgagcaggat taaaattggc ttaaggaatg tgatatgaaa
     6961 aatgacgcag acagttacac cgtttaaatg caataatcag ccacgtttct cgttaataac
     7021 aataccagta cctggtttgc gcaaggcgaa ggattatttt tatgaagctt aagaacaccc
     7081 tcctggcgtc ggcactgctt tctgctacgg cattctccgt taacgcagca acagaactga
     7141 caccggagca agcggcagcg gttaaacctt ttgaccgtgt agtggttacc ggtcgtttta
     7201 atgctattgg cgaagcggtg aaagccgttt ctcgtcgcgc agataaagaa ggtgccgcct
     7261 ctttttatgt tgtcgacact tctgattttg gtaacagcgg taactggcgt gtggtcgctg
     7321 acctctataa agccgatgct gaaaaagcag aagaaacaag taatcgcgta attaacggtg
     7381 ttgtcgaact gccgaaagat caggctgttc tgattgaacc gtttgacacg gtcaccgtcc
     7441 agggcttcta tcgtagccag ccagaagtca atgatgccat caccaaagcg gcaaaagcga
     7501 aaggtgccta ctctttctac atcgttcgtc aaatcgatgc caaccaaggc ggcaaccagc
     7561 gtattactgc attcatctat aaaaaagatg ctaagaaacg tatcgtccag agcccggatg
     7621 tgatcccggc agattccgaa gcaggacgtg cagctctggc tgccggtggc gaagccgcga
     7681 agaaagttga gatcctaggt gttgcgacta ccgcatcacc aagttctgaa gtcggtcgtt
     7741 tctttgaaac ccagtcatca aaaggcgggc gttacaccgt catgctcccg gatggcacta
     7801 aagtcgaaga actgaacaaa gcgaccgcag cgatgatggt cccgttcgac agcatcaaat
     7861 tctctggcaa ctacggcaac atgaccgaag tctcctatca ggttgcgaaa cgtgccgcga
     7921 agaaaggtgc taagtattac cacatcaccc gccagtggca ggaacgtggt aataacctga
     7981 ccgtcagcgc agatctgtat aaataactat tcagttactc ctataaggcg gcttgatgcc
     8041 gcctttcgca tttttagtca caaatttccc cgcgaccatt gcatgcagac acatctcttc
     8101 gtaaaatccc gcgcttcagt ggcaaactcc atttttatgc gttttgccct aatatttatt
     8161 cttcttatca cgttttaatc actggatatc gatgaaaaag aaactgggac tgagcgcact
     8221 caccgcgctg gtattaagct caatgctggg cgcgggtgtt ttcagtctgc cgcaaaatat
     8281 ggcggcagtt gccagcccgg cagcactgct catcggctgg ggtattactg gcgctggcat
     8341 tttattgctg gcctttgcca tgctgatcct cacgcgcatt cgtcccgaac ttgacggcgg
     8401 tatctttacc tatgcccgcg aagggtttgg cgagctaatc ggcttttgtt ccgcatgggg
     8461 atactggctg tgcgcagtca tcgccaacgt ctcctatctg gtgatcgttt tttccgcgtt
     8521 aagctttttt acggacacgc cggaattgcg cctgttcggc gatggtaata cctggcaatc
     8581 gatcgtcggt gcatcggctt tattgtggat tgttcacttt ttgatcctgc gcggggtgca
     8641 aaccgctgcc agcattaacc tggtggcgac actggcaaaa ttgttgccgc tgggtctgtt
     8701 tgttgtgctg gcgatgatga tgttcaaact ggataccttc aagctcgact tcaccggact
     8761 tgcacttggc gtacccgttt gggaacaggt gaaaaacacc atgctgatca ccctgtgggt
     8821 tttcattggt gtggaaggtg ccgtggttgt ttctgcgcgt gcgcgtaata aacgtgatgt
     8881 tggcaaagcg acactgctgg cggttctctc cgctctgggc gtttacttgc tggtaacgct
     8941 gctttcgctg ggtgtggtgg cacgtcctga actggctgaa attcgtaacc cgtcaatggc
     9001 cggtctgatg gtggaaatga tgggaccatg gggcgaaatc atcatcgctg ccggtttgat
     9061 cgtttccgtt tgcggcgcgt acttaagctg gaccatcatg gcggcagaag ttccgttcct
     9121 ggctgccact cataaagcat tcccacgcat ttttgcgcgc cagaacgcac aagctgcgcc
     9181 atccgcctca ctgtggttga ccaacatctg cgtgcaaatt tgtctggtac tcatctggct
     9241 gaccggttcc gattacaaca cgttgctgac catcgcctca gaaatgattc tggtgcccta
     9301 tttcctggtc ggcgcgttct tgctgaaaat cgcaacccgt ccactgcata aagcggtagg
     9361 tgtcggtgcc tgcatttatg gcttatggtt attgtacgcc tctggtccaa tgcacttgct
     9421 gttatccgtt gttctgtatg cgccggggct gctggttttc ctgtatgcgc gcaaaacgca
     9481 tacacatgat aatgtactga accgtcagga aatggtgctg attggcatgc tgttaattgc
     9541 ctcagttccg gcgacctgga tgctggtggg ataacgttca tcccatcgtt ggatagcaaa
     9601 ggagataacg atgggtaaag cccagccctt gccaatatta attactggcg gaggtcgtcg
     9661 catcggcctc gccctcgcct ggcatttcat taatcaaaag caaccggtga ttgtcagcta
     9721 tcggacgcac tatccagcca ttgatggact gattaaggca ggtgcgcagt gtattcaggc
     9781 tgatttttcg accaacgacg gtgtgatggc gtttgccgat gaagtactaa aaagccccca
     9841 tggtctgcgt gctattttgc ataacgccag tgcgtggatg gcggaaaaac cgggtgcgcc
     9901 actggccgac gtactggctt gcatgatgca gatccacgtt aataccccat acctgctcaa
     9961 ccatgcgctg gaaagattac tgcgtgggca cggacacgcc gccagcgata tcattcactt
    10021 taccgattat gtggtggagc gcggtagcga caagcatatt gcgtatgctg caagcaaagc
    10081 ggcactggat aatatgaccc gctcgtttgc ccgcaagctg gcaccggaag tgaaagtgaa
    10141 ctctattgct ccatcgctga tcctgtttaa tgaacatgat gatgccgaat atcgacaaca
    10201 ggcgctgaat aaatcactga tgaaaaccgc gcctggcgag aaagaagtga tcgacctggt
    10261 cgattactta cttaccagtt gctttgtcac cggacgcagt ttcccacttg atggcggtcg
    10321 tcatctgcgt taatgcagct ttatccagca tataatcaac acccatgcgc ttatccccca
    10381 acacgaaaca gaaccaacaa aagcggcggg caagcgcatc attccggtga aataccacag
    10441 cgacaccagg taaacaaaat agggaataat cgaccacata ctaaaaatga tggttgcgcg
    10501 taaggcttca atgccgcgtt cgctggcaac aatataatgc gcgataagcg caaaggtcgg
    10561 gaaaagtgga atcagcccgg cgatataata atttttcgtt tttgctaaaa caccaatcaa
    10621 cagcactacc agcgcaccga gcgcggcttt gattaccagc cccatttttt taccttaaca
    10681 cttccataac aagtcatcag tagaatacct gatgaaaact tgtttagaaa cgattgatag
    10741 taagtaaaaa cagcgcggtg tattgtgacg tttttatatc taccgtgaat gttatgaaca
    10801 ctatcgtatt tgtggaagat gatgcggaag tcggttcact gattgccgcg tacctggcaa
    10861 aacatgatat gcaggttacc gtagagccgc gcggcgacca ggccgaagaa accattttgc
    10921 gagaaaatcc ggatttggtg ttactcgaca tcatgctacc aggcaaggac ggcatgacca
    10981 tttgtcgtga tttacgcgca aagtggtctg gaccgattgt tcttctaacc tctctcgata
    11041 gcgatatgaa ccacatcctg gcactggaaa tgggtgcctg cgactatatt ctcaaaacga
    11101 cgccccctgc tgttttgcta gcgcgtttac gtttgcattt gcgtcagaat gagcaagcca
    11161 cactgaccaa aggtcttcag gaaacgtctc tgactcccta caaagccctg catttcggca
    11221 cgttgaccat cgatcccatc aaccgcgtag tcaccctggc taacactgaa atctcgctct
    11281 cgacagctga tttcgaatta ttgtgggaat tagctaccca tgccgggcaa atcatggacc
    11341 gcgatgcatt gctgaaaaat ttacgcggcg tcagttatga cggactggat cgtagcgtgg
    11401 acgtggctat ttcgcggtta agaaaaaaac tgctcgataa cgccgcagaa ccttatcgca
    11461 ttaaaactgt gcgtaacaaa ggctatcttt ttgcgcctca tgcatgggaa taagcgatga
    11521 aaaaactgtt tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc
    11581 tggttgggct ggtgtacaaa tttaccgccg aacgcgcggg caaacagtcg ctggatgatt
    11641 tgatgaacag ttcactgtat ctgatgcgca gcgaattgcg tgagatcccc ccacacgact
    11701 ggggtaagac gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccac
    11761 tgagtaaata ccatcttgat gatatttcca tgcaccgact gcgtggcggc gaaattgtcg
    11821 ccctggacga tcagtacacg tttttgcagc gtatcccgcg cagccactac gtgctggcag
    11881 ttggtcctgt tccttatctt tattatctcc atcagatgcg attgctggat atcgccctga
    11941 tcacttttat tgctatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc
    12001 aggatatgtt aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac
    12061 gtatccactt tgatgagggt tcgagctttg aacgacttgg tatcgcattt aaccagatgg
    12121 cggacaatat caacgccttg attgccagca aaaaacagct tattgacggt atcgctcacg
    12181 aactgcgaac accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg
    12241 ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact tgaagcttta attgaagagc
    12301 tgctgactta tgcccgactc gatcgcccac aaaacgagct tcatcttagc gaaccagacc
    12361 tgccgttgtg gctgtcaacg catctggcag acattcaggc agtaacgccc gataaaacgg
    12421 tacggattaa aacgctcgcg caaggccatt atgcggcgtt ggatatgcgc ttaatggagc
    12481 gcgtgctgga taatttgctc aataacgccc tgcggtactg ccattcaacg gttgaaacca
    12541 gcctgctact gtcggggaat agagcgacat taattgttga ggatgatggc ccagggattg
    12601 ccccagaaaa ccgcgaacat atctttgaac cttttgttcg cctcgatccc agccgggatc
    12661 gctcaaccgg cggctgcggg ctggggctgg caattgtcca ctctatagca ctggcaatgg
    12721 gcggtacggt taattgtgac accagcgaac tgggtggtgc tcgcttctcg tttagctggc
    12781 cgttatggca taacatcccg caatttacct ctgcctgaca ctacgcgcac gatggtcaag
    12841 tcaccaccac tgtgctataa aataagtatg ttgtaactaa agtggttaat attatggcgc
    12901 gttacgatct cgtagaccga ctcaacacta cctttcgcca gatggaacaa gagctggctg
    12961 catttgccgc tcatcttgag caacacaagc tattggttgc ccgcgtgttc tctttgccgg
    13021 aggtaaaaaa agaggatgag cataatccgc ttaatcgtat tgaggtaaaa caacatctcg
    13081 gcaacgacgc gcagtcgctg gcgttgcgtc atttccgcca tttatttatt caacaacagt
    13141 ccgaaaatcg cagcagcaag gccgctgtcc gtctgcctgg cgtgttgtgt taccaggtcg
    13201 ataacctttc gcaagcagcg ttggtcagtc atattcagca catcaataaa ctcaagacca
    13261 cgttcgagca tatcgtcacg gttgaatcag aactccccac cgcggcacgt tttgaatggg
    13321 tgcatcgtca tttgccgggg ctgatcaccc ttaatgctta ccgcacgctc accgttctgc
    13381 acgaccccgc cactttacgc tttggttggg ctaataaaca tatcattaag aatttgcatc
    13441 gtgatgaagt cctggcacag ctggaaaaaa gcctgaaatc accacgcagt gtcgcaccgt
    13501 ggacgcgcga ggagtggcaa agaaaactgg agcgagagta tcaggatatc gctgccctgc
    13561 cacagaacgc gaagttaaaa atcaaacgtc cggtgaaggt gcagccgatt gcccgcgtct
    13621 ggtacaaagg agatcaaaaa caagtccaac acgcctgccc tacaccgctg attgcactga
    13681 ttaatcggga taatggcgcg ggcgtgccgg acgttggtga gttgttaaat tacgatgccg
    13741 acaatgtgca gcaccgttat aaaccacagg cgcagccgct tcgtttgatc attccacggc
    13801 tgcacctgta tgttgcggat taacgcccgg ctttcatact gccgaccatc tgttctggcc
    13861 gtacccagct gtcaaactcg gcttcgctaa gataccccag cgcaagggcc gcagctttta
    13921 aggtcagccc ttctttatgc gcttttttgg cgatctccgc ggctttgtca taaccaatgt
    13981 gggtgttaag cgcagtcacc agcatcagcg attcattgag taattgattg attcgctcac
    14041 gattcggttc aatacccact gcgcagtgtt tgttaaaact ttccatgcca tctgccagca
    14101 agcgcaccga ttgcaggaaa ttgtggatca ccattggacg gaagacgttc agttcaaagt
    14161 taccggaagc gccacccatg ttgatcgcca cgtcgttccc cattacctga cagcagagca
    14221 tggttaatgc ttcacactgg gttgggttca ctttccccgg catgattgag ctgcccggct
    14281 cattttccgg gattgagatt tcaccaattc cgcagcgcgg gccagaggcc agccagcgga
    14341 catcattggc gattttcatc agtgacgcag ccaacccttt caacgcgccg tgcgcctgaa
    14401 ccagggcatc acaggtcgca agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac
    14461 aggtaatgac tgccagttca tctgctacgc gacgcgcata ctccggatgg gtatttagtc
    14521 cagtacccac cgctgtaccg ccgagagcca gttccgctac gtgaggcagg ctgtattcga
    14581 tatgtttgag attatgctcg agcatcgcta cccagccgga aatctcctgc cccagtgtta
    14641 gcggcgtggc gtcctgcaag tgggttcgac cgattttgac gatatcggca aatgcacgcg
    14701 atttttcact cagtgtctgt gtcagggttt taacctgcgg aatgagttgc ttgcgcagcg
    14761 ccagcagcgc cgcaacgtgc atcgccgtcg gaaagacatc gttggaactt tggcttttgt
    14821 tcacgtcgtc gttagggtga actttacgtt ccatcccgcg cacgccgccg agtaattcac
    14881 tggcccggtt agccagcact tcgttcatgt tcatgttact ttgcgtgccg gagccggtct
    14941 gccagatagc cagcgggaat tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct
    15001 gccgaatggc gctcgctttc tcttcagaca acaagcctaa atcttcatta acttttgccg
    15061 ctgcgcgctt ggttagcgcc agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa
    15121 tgcggaaatg ctccagcgag cgttgagttt gtgcgcccca cagcttatct gccgggacat
    15181 caatcgcccc catcgaatct ttttcgctgc gtactgtatt catggcctgc tcctcacctg
    15241 attaattttt tctttctgtt ttgccttcgt taagcaactt ttagctcact tattatttac
    15301 tatttgataa caaatgtttg gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc
    15361 tctgtttatt tcacacagcg agtgcactgt gtgagttgta tctgctggaa gaagtcattt
    15421 cctttatcat ccacaaggat aaacgccggg aaatcttcca cttcaatttt ccagatggct
    15481 tccattccca gttccggata ttcaacacat tccaggctct taatacttcc ctgcgccaat
    15541 acagcggccg gaccaccgat actgccaagg tagaagccgc cgtgtttttt acaggcatcc
    15601 gtcacctgct ggctgcggtt gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc
    15661 agttgatcga cataagaatc catccgtccg gcggtcgttg ggccaagaga accggaggca
    15721 taaccttccg gcgttttggc cggacccgcg tagtaaatcg gatgatcttt aatgtactgc
    15781 ggcagccctt caccgttatc catccgctct ttcagtttgg cgtgagcaat atcacgaccg
    15841 acgataatcg tgccgttaag cgataagcgt gtagaaacgg gatactgcga caactgtgcg
    15901 aggatctctt tcatcggacg gttaaggtca acgcgcaccg cttcgccttc tcccgcttta
    15961 cgcagctctt cagggatata tttacctgga ttatgttcca gtttttcgat ccagatcccc
    16021 tgacggttga tcttcgcttt gatattacgg tcagcagagc aggaaacacc cataccaacc
    16081 ggacaggatg cgccgtgacg tggcaggcga atcacgcgta tgtcgtgagc gaagtattta
    16141 ccaccaaact gcgcacccag accgagattt tgcgcttcga tcagcaattc tttttccagt
    16201 tccacatcgc ggaatgcctg accgtgctca ttcccttccg ttggcagttc gtcatagtat
    16261 ttagcggaag ccagtttcac tgttttaagg ttagtttctg cagaagttcc accaattacg
    16321 aacgcaatat gatacggagg acaggccgcc gtacccagcg tgcgcatctt ctcaaccagg
    16381 taatttttca gtttccccgg cgttagtaac gctttggttt cctgatagag atacgtcttg
    16441 tttgccgaac caccaccttt ggcgatacag aggaatttgt actcgtcgcc atcaacggca
    16501 taaagatcga tctgcgctgg cagattggtg ccggtattca cctctttata catatccagc
    16561 ggcgcgtttt gcgagtagcg caaattatct tcgatataag tgttatagac accgcgcgcc
    16621 agcgccgctt catcacagcc accggtccat acacgctgcc cttttttacc aacaataatc
    16681 gcagtgccgg tatcctgaca ggttggcaga acacctttcg ccgcgatgtc ggagttgcgc
    16741 aggaattgca gcgccacata tttatcattt tcgctggctt ccgggtcacg cagaatgtcg
    16801 gccacctgtt gttggtgagc cggacgcagc ataaatgacg catcatgaaa cgcctgacgc
    16861 gccaacagag ttaacgcttc gggtgcgact ttcaaaatct cctgcccttc aaattcagat
    16921 acgctaacgt gttcgctggt tagcaggtaa tactcagtat catctttttt gagtggaaaa
    16981 ggagcctgat aatgaaaggg tttgtttgac attgttctct cacttactgc ctggtttggt
    17041 tatgctctgg gcgggtgttc cgttgccctg ttaaaagcga gtaacaatat cctacacact
    17101 tttttaacaa aaactgagac tagtacgact ttttgcggct ccaggttact tcccgtagga
    17161 ttcttgcttt aatagtggga ttaatttcca cattaaaaca gggattgatc atgcaaaaac
    17221 tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg
    17281 gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa
    17341 gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga
    17401 gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc
    17461 tgttcaaagt attatgcgca gcacagccgc tctccattca ggttcatcca aacaaacgca
    17521 attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc
    17581 gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg
    17641 cgatgaacgc gtttcgtgag ttttccgaga ttgtctccct actccagcca gtcgcaggtg
    17701 cacatccggc aattgctcac tttttacaac agcctaatgc cgaacgttta agcgaactgt
    17761 tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat
    17821 cggcgctcga cagtcagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt
    17881 acccggaaga cagtggcctg ttctccccgc tgttactgaa tgtggtgaaa ttgaatcctg
    17941 gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg
    18001 aagtgatggc gaactccgat aacgtgctgc gtgcgggtct gacgcctaag tacattgata
    18061 ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc
    18121 agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct
    18181 cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt
    18241 tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg
    18301 gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt
    18361 tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag
    18421 cttattaaag gcttataaca ccttcaggcg gccagtccgc ctgatttcat tttatggata
    18481 atcattatga ataaatcgct ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg
    18541 acaggcggcg catggtatac aggcaagaag attgaaaccc atctcgaaga catggtcgcg
    18601 caggcgaacg cgcaactcaa actgacagct cctgaatcca acctggaagt gagttatcaa
    18661 aactatcatc gcggcgtatt cagcagccag ttgcaactgt tggtgaaacc cattgccggg
    18721 aaagaaaatc cgtggattaa aagcggtcag agcgtcatct tcaacgaatc ggttgatcat
    18781 ggtcccttcc cgcttgccca gcttaaaaaa ctgaacctga tcccgtcgat ggcatcaatt
    18841 caaaccacgc tggttaataa cgaagtaagc aaaccactgt ttgatatggc aaaaggtgaa
    18901 acgccttttg agattaactc gcgcattggt tacagcggtg attccagttc cgatatttcg
    18961 ctcaagccac tgaattacga gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc
    19021 cagttaaatg ctgacagaga cggcaaagcc atctcccttt ccggggaggc gcaaagtggt
    19081 cggatagacg cagttaacga atacaaccag aaagtgcagt tgacctttaa taatctgaaa
    19141 accgacggtt ccagcacgct ggcaagtttt ggtgagcgcg taggaaacca aaaactgtca
    19201 ctggaaaaaa tgaccatttc agtggaaggc aaagaactgg cactgctgga aggcatggag
    19261 atcagcggta aatcggatct ggtcaatgac ggtaaaacga tcaatagcca actggattac
    19321 tcgctaaaca gcctgaaggt acagaatcag gatctgggca gcggcaagct gactttaaaa
    19381 gtcggccaga ttgatggtga agcctggcat cagtttagcc agcaatataa cgcgcaaact
    19441 caggcgctgc tggcacagcc agaaattgcc aacaatcccg aactttatca ggagaaagtg
    19501 acggaagcct tctttagcgc cctgccgctg atgttgaaag gcgatccggt gattactatc
    19561 gcgccgctaa gctggaaaaa cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg
    19621 aaagatccgg caacgactaa agaagcgccg caaacgctgg cgcaggaagt agatcgttcg
    19681 gttaaatctc tggatgcgaa actgaccatt ccggtggata tggcaactga gtttatgact
    19741 caggtagcga agctggaagg ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa
    19801 gttgaaggtg catcagcaat ggggcagatg ttccgtctga ccaccttgca ggacaatacc
    19861 atcaccacca gcctgcaata tactaacggt cagataacgt taaacgggca gaaaatgcca
    19921 ctggaagatt tcgttggtat gtttgcgatg ccagctctta acgttccggc tgtaccggca
    19981 attccgcagc agtaattcac ggccaaaacg ccggagaatt ttcacttcgg cgttttcatc
    20041 tgaaaacaaa ccagagattg cctgatgcgc tacgcttatc aggcctacct tttcctgcaa
    20101 cactttgaat ttatgagttt ttgtaggctg gataaggcgt ttacaccgca tccggcataa
    20161 aaaacgcgca ctttgtcaac aatctgaaac gccggaggtt tttctctccg gcgttatttc
    20221 tgacttcagc ataaagtcat actgaatact cattttcaca tcccagtaat tcaggcgatc
    20281 gcccagcgta ctgccatcgc gcttttcccg ccctttatcg gcagcaagaa agaaattcat
    20341 cccttgtaac ggcccttcgc tgacccgata gttcatacca cctaagatac ccgcgccttc
    20401 aatccgcaca tctcgtttac tggattcata gccgcgagtc acaatctgtg caaatgtcag
    20461 tgtaaattgc ggcgttaaac gatagttgac gcccaattgc caggactgca ttccttcacg
    20521 gttacgatca agacttaatg aaaagggata gccaatgtcg gtatccacca cggcaccatt
    20581 cattgcatgg cgcaaataac caccactgcc aaatacgctt ccccacggcg cattccaggt
    20641 caattgtcca ctaaccaacg ccgtttcatt gggctggctg gtattacgac ttaaaccttc
    20701 gagctgcgcg taaaagccga gcaactcccc tttcaacgta taattcgggt cgagcggtat
    20761 gcttttctcg agaatacttt gtatctgatt ggaaacatct ttctgtaccg atgcccccag
    20821 agaatagcga ctggtatcat catgccaact ggcggctaaa aaagaacgtg cgcgatgttt
    20881 atcgtatttt cccgtctggc tgttatacac cccttcatca cgaataccgg ttgttgaatc
    20941 agtccataaa aatactcgtg aacgccacat tccccacaga tcaatatttg gtgttaaagc
    21001 ccaataacct aatgctcctt cagaaactgt cggtaatgta cgttgcgaac tgccagccat
    21061 taacggaata cgataaggta agctaccata actataatcc gtcccgaagc gacctgattt
    21121 aagcaggcta ttttcactgg gttttatttt tactgccccc agggcaaagc caaaactttt
    21181 atcaccatca agataccacc gggtactcat atcagcacga gcacctaatt tataaacata
    21241 atatgccccg ccttcaacgc caataatatt ggaataataa ccactattga aatcgagcaa
    21301 tcctccctgt acccatgcgt aaatatctct tcccgcgcca gcactgggct tatccgccct
    21361 gcgcaattcg tttttcagtc tgatgcgtag tcccgcctca tcgtcagcca gttgcgccgc
    21421 ataagcagag ataaggccag aggcagccgt caggcaaata acggccatgg ccactatttt
    21481 tctcatagtt gcacctttga tgttctgatt atttttattg attattaatt agtgatatcg
    21541 ctgattaatt gctgctgcac ttttttacga ttatcaattt caaccacgat ttctttgaat
    21601 tttttatccg taagcggata aaaccagata ataacgaatg ccagtagcat aaatccgcaa
    21661 ggtactaagg caattgatgt gcggatgccc ataataactt ccggcgtttg cacctgattg
    21721 gcaatatatc cgcttaaccc caaaataaag gcaggaattg aacctccgat tgcctgaccg
    21781 catttacggg taaatgagaa tagtgaatag gtgagccctt caattcgcac gccggtcagg
    21841 tattcaccgt attctacggt atcagcttcc agtgcccaca tcactgtcat agtaacgccc
    21901 tgaccaattg aagcgatggc caacgcaacc aacgccaccg gcagtgacca gacggaaacc
    21961 cagaagaata gcagataacc gcaggttccc agcaaagcgc caatcaggaa ggtattcttt
    22021 ttaccgatcc tcgcgaccat ccccggcaca agcggtgccg atgccacagt accaaccagg
    22081 ttttgcacca gtaccagcac agtgaacagc ccggtatcat ttaacacata gcgcacgtag
    22141 aacaacgacg aggcgctgac cgcaaaggtc gaaatcagca cacacagcgc accgatgcac
    22201 aacataaaca gcgggcgatt ccgtttcagg gtttgcaaac tgatcttcaa tgacggctgc
    22261 gcaacgatac gtaccacatt ctcacgcgtc gatttgaagc agatgaagta aagcaccatt
    22321 ccggcaatcg ccagcacaat cgtccagaaa tggtataccg acaccatctc ttccgggctg
    22381 gagttcttaa tgctcggccc tatcagaaat gccaggcaga caaaggtcaa tgaagcggca
    22441 atcccacgag ccgcgcccag acgggcgcgg gattgtggtt gttgggtcat cgcggtagca
    22501 agtgaaccat aaggaatatt caccaggctg tagcaaaacc cgaggcccat gtaggtcaaa
    22561 tatgcataca ctactttgcg gctatggctc cagtcggtcg gtacccagaa taccagcacg
    22621 ctgaagatca ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg
    22681 gtattcacac tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc gaatacccgc
    22741 accagtaaca gcatggtgcc cgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa
    22801 ctcaacagga agagcacccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg
    22861 tagccgacga tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt
    22921 tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt
    22981 tgcggtcgcg ggtgaagatc cccttcttgt taccgccaac gcgcaatatg ccttgcgagg
    23041 tcgcaaaatc ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga
    23101 cgcggtgata catatccagc catgcacact gatactcttc actccacatg tcggtgtaca
    23161 ttgagtgcag cccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca
    23221 gtttctcctg ccaggccaga agttcttttt ccagtacctt ctctgccgtt tccaaatcgc
    23281 cgctttggac ataccatccg taataacggt tcaggcacag cacatcaaag agatcgctga
    23341 tggtatcggt gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt
    23401 cgagtttacg cgttgcttcc gccagtggcg cgaaatattc ccgtgcacct tgcggacggg
    23461 tatccggttc gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta
    23521 tcagctcttt aatcgcctgt aagtgcgctt gctgagtttc cccgttgact gcctcttcgc
    23581 tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc
    23641 cgacagcagc agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct
    23701 cttcagcgta agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg
    23761 cgtggtcgtg caccatcagc acgttatcga atcctttgcc acgcaagtcc gcatcttcat
    23821 gacgaccaaa gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca
    23881 ctgccactga ccggatgccg acgcgaagcg ggtagatatc acactctgtc tggcttttgg
    23941 ctgtgacgca cagttcatag agataacctt cacccggttg ccagaggtgc ggattcacca
    24001 cttgcaaagt cccgctagtg ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca
    24061 gttcaacgct gacatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt
    24121 cttgcgcgac atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga
    24181 gcattacgct gcgatggatc ccggcatagt taaagaaatc atggaagtaa gactgctttt
    24241 tcttgccgtt ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt
    24301 tgttcacaca aacggtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg
    24361 cttcaaatgg cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt
    24421 tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg
    24481 gtataaagac ttcgcgctga taccagacgt tgcccgcata attacgaata tctgcatcgg
    24541 cgaactgatc gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt
    24601 cccaccaacg ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt
    24661 cgagtttttt gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat
    24721 taagataaaa atactggtca acctttaatc tgattagtta accaattctg tgttgcacac
    24781 agttatctgt gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc
    24841 aaaaaaccaa aaactgcaat acccacagcg tgatattgat cgcattaatc tttaaaacgg
    24901 cgttgacaga agccataaaa taagcaaccg aacgttcgtt cgagcatatt gggaacgata
    24961 aactctcatg acatatggct acggtgaata tttttggggg ggaattctcc gatcccggct
    25021 agattgtatt ttgtacaggg aaattaaagt atgaaacgcg ggcacagtta tcaccagccc
    25081 gcgaaatgag aggatcagga tgcggttaag ataccgccaa tcatcgcgcg taatccctga
    25141 gcaaacgacg cttcacgcgc cagatcttcc gcttcgatat cggccagtac accgtaggtc
    25201 agatccagca gtaaacgtga agtcattgac gtgttgatgt ctgggcttat ctcgcctttt
    25261 tgctgggcat cacgcatccg ctgggcaaca aattccgtga tcgtcatatg cttattttta
    25321 agcatggcgg cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc
    25381 cgttgcccaa aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa
    25441 tgaatccctt taatcggttc ccggaaacga gccagcgccc tctcctggtc ctgtaagata
    25501 atcgcctgaa tcaaggcttc tttggagatg aaatggtgat agagcgtccc gggactaatg
    25561 gcgcaagatt tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc
    25621 tctctggcag cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc
    25681 atcattaaac cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc
    25741 tatcattgct gttgaatagt gcgaaggcac actctattcg tatacttttc actctgaaat
    25801 tggttaacca atagccgtga tttggctgcc agttatttag cagagacgtt gatgagttta
    25861 tcccggtaaa caacacgcac ggttgtttac cggtccgtta gtgtattaat tgagctcctg
    25921 taccccacca ccggagacgg tgagaatttg tccgcttacc cagctcgcag caggcgagca
    25981 aaggaacagc gctgcgttag caatatcttg cggttggccc agacgtctga tcggcgtgtg
    26041 ctgtaacatt ttttgttcaa tttctggtgt aataacggat ttcagggcat cggttaatat
    26101 tgcccccggc gcaatgccat ttacccgaat atttttttca cccaggtcaa acgccatatt
    26161 tctgaccaga tgactggctg cagctttaga tgatgcatag gaagtcatgt ttatattttt
    26221 attttctgcc gccatagaag tgatggtcag aataacgcca ccgccatttt tttccatttc
    26281 tggcgcaaca agttgtgaca gatggaaaaa agaaaacaca ttcagttcat aagcacggcg
    26341 aaaatccgcc attggcatat caaacggttt aggtccaccg ccaccggcgt tgttaaccag
    26401 aatatcaact ttacccagct tactgatagc aaagtctgcc agtgcagaga gttcctgttc
    26461 ggaagtaata tcacaacggc aggcaaatgc ctgaccaccc agttgttgaa tttcgtctac
    26521 aacatggtta gctgcgtcgg cgttaatatc actgaccacc acagatgcgc cagctgtcgc
    26581 gaatgtaatg gcgatttctt taccaatacc tgcacccgca cctgtgat
//
LOCUS       DAJVXP010000046        26570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVXP010000046 DAJVXP010000000
VERSION     DAJVXP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.046"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..296
                     /locus_tag="OQS10_001857"
     CDS             <1..296
                     /locus_tag="OQS10_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCS2292893.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="OQS10_001858"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="OQS10_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS2292894.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="OQS10_001859"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="OQS10_001859"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS2292895.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="OQS10_001860"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="OQS10_001860"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS2292896.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="OQS10_001861"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="OQS10_001861"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS2292897.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="OQS10_001862"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="OQS10_001862"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS2292898.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="OQS10_001863"
     CDS             7366..8796
                     /locus_tag="OQS10_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS2292899.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="OQS10_001864"
     CDS             8895..9908
                     /locus_tag="OQS10_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS2292900.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="OQS10_001865"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="OQS10_001865"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS2292901.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="OQS10_001866"
     CDS             complement(10732..11055)
                     /locus_tag="OQS10_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS2292902.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..13035)
                     /locus_tag="OQS10_001867"
     CDS             complement(11266..13035)
                     /locus_tag="OQS10_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS2292903.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13684..14199
                     /locus_tag="OQS10_001868"
     CDS             13684..14199
                     /locus_tag="OQS10_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2292904.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14417..15145
                     /gene="artP"
                     /locus_tag="OQS10_001869"
     CDS             14417..15145
                     /gene="artP"
                     /locus_tag="OQS10_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS2292905.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            15163..15894
                     /gene="artJ"
                     /locus_tag="OQS10_001870"
     CDS             15163..15894
                     /gene="artJ"
                     /locus_tag="OQS10_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2292906.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15901..16617
                     /gene="artQ"
                     /locus_tag="OQS10_001871"
     CDS             15901..16617
                     /gene="artQ"
                     /locus_tag="OQS10_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS2292907.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16617..17285
                     /gene="artM"
                     /locus_tag="OQS10_001872"
     CDS             16617..17285
                     /gene="artM"
                     /locus_tag="OQS10_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS2292908.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17577..18308
                     /gene="artJ"
                     /locus_tag="OQS10_001873"
     CDS             17577..18308
                     /gene="artJ"
                     /locus_tag="OQS10_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS2292909.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="OQS10_001874"
     CDS             complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="OQS10_001874"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS2292910.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19651..20139)
                     /locus_tag="OQS10_001875"
     CDS             complement(19651..20139)
                     /locus_tag="OQS10_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS2292911.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20199..21044)
                     /gene="potI"
                     /locus_tag="OQS10_001876"
     CDS             complement(20199..21044)
                     /gene="potI"
                     /locus_tag="OQS10_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS2292912.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(21041..21994)
                     /gene="potH"
                     /locus_tag="OQS10_001877"
     CDS             complement(21041..21994)
                     /gene="potH"
                     /locus_tag="OQS10_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS2292913.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(22004..23137)
                     /gene="potG"
                     /locus_tag="OQS10_001878"
     CDS             complement(22004..23137)
                     /gene="potG"
                     /locus_tag="OQS10_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS2292914.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23232..24344)
                     /gene="potF"
                     /locus_tag="OQS10_001879"
     CDS             complement(23232..24344)
                     /gene="potF"
                     /locus_tag="OQS10_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS2292915.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24695..25171)
                     /locus_tag="OQS10_001880"
     CDS             complement(24695..25171)
                     /locus_tag="OQS10_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS2292916.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="OQS10_001881"
     CDS             complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="OQS10_001881"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS2292917.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26222..>26570)
                     /gene="nfsA"
                     /locus_tag="OQS10_001882"
     CDS             complement(26222..>26570)
                     /gene="nfsA"
                     /locus_tag="OQS10_001882"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS2292918.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         6556 a         6922 c         6569 g         6523 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt cactccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tctaaacgaa acatttttga atacattatt
    11821 tattatattg ctatttgtta ttttaacatc acctaaactg ccgttttcaa aacaacattt
    11881 tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta tgttttgctt
    11941 tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat tgagtagtgt
    12001 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
    12061 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
    12121 atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag gaaacagatt
    12181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
    12241 tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat ctaacgcagt
    12301 ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
    12361 gtcataaaaa gtgttcatca atgcgatatt gtttgttaca gcgtcttttt cgatattctc
    12421 actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct ttataaagga
    12481 tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa tcacattcgc
    12541 aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
    12601 gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact gttgtctttc
    12661 aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac ccgcctttat
    12721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
    12781 actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga gcaacacatc
    12841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
    12901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
    12961 atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg cttgatcaga
    13021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctcgt
    13081 gctttataat tataaaagca caacgccttt tatattcata ttaattaacg agatactgaa
    13141 acaacttaat ttattcttcg ctaaaattca ttagcttaaa ttctttagcc acaattgtag
    13201 cataaataat attatcgaga gtcaaagctg ggactatcaa atttcgctca ttttcaaaaa
    13261 tacaacgtaa ttttggcaat catcttaatc gcaggatctc ttatcgcatg tgaaaaattt
    13321 tgtaatgatt agacaataac cgcgttcatt tatcacgcgt tataaatgtg aaactggctt
    13381 cttgcagatg ctcttaacct ctacaggagg atttatacca ccatttcaga actatcatat
    13441 ttaccttaaa tgatcatttc agccatataa cacgctcaac ctcacaaatg gtttacactg
    13501 tctattgttt gggtaattta agtaagtcag ctacgagcaa ccttgagtgg atagccagca
    13561 atggactcaa acccaatcag gtgttcagat tgtttcagaa ttgtattaaa aacaatgtat
    13621 taaataatct gatagcgttt ggtcgtagtg tgacgcagac agcaagctaa taaggaaatg
    13681 attatgcgct accgttcact atctctactt attccatgtg cactgctgct gagtgcctgt
    13741 accaccgtca cgccagctta taaagataac ggtccccgca ccggttcctg tgtccagggg
    13801 gggcctgaca gcgtggctca acaattctat gactaccgca ttcaacatcg cagcaatgac
    13861 attaccgccc tgcgccccta tttgagtgac aaactggcaa cccttcttag cgatgccagc
    13921 cgcgataata gtcatcgtga attgttaagc agcgatccgt tctccagtcg aaccaccttg
    13981 ccagatagcg cccacgttgc cagtgcatcg actatcccta atcgtgatgc ccgtaacatt
    14041 ccattgcgcg tagatctcaa acagggtgac cagggttggc aggatgaagt gttaatgatt
    14101 caggaaggcc agtgttgggt catcgacgat gtgcgctact taggcggcag cgttcatgcc
    14161 acggcgggta cgctccgtca gtccatcgag aatcgctaac gccttgatca accaataaac
    14221 cccgtaaatt gtccggcatt tcgtgaggaa tgccgacatt tatgctcgcc gaccaccgcc
    14281 cccgttattt tgtgctatgt ttattgaata atgcgctttg cttttaactt ttaaagcaga
    14341 aatattgcat aattattctg tcaaaggtac tatcttcgac ctcaatagct atcagactgc
    14401 cagtatacga gtgtcaatga gtattcaatt aaacggcatt aattgcttct acggcgcgca
    14461 tcaggcgctg ttcgatatca cgctggattg cccacagggc gaaacgctgg tgttacttgg
    14521 ccccagcggc gcgggtaaaa gctcgctgct gcgtgtactc aatctgcttg agatgccacg
    14581 ctccggtacg ctcaacattg caggcaacca tttcgatttc accaaaacac cctctgacaa
    14641 agcgattcgc gatttgcgtc gtaacgttgg catggtgttt cagcaataca acctgtggcc
    14701 gcatctgacc gtgcagcaaa acctgattga agcgccctgc cgtgtactgg ggttgagtaa
    14761 agatcaggcg ctggcccgtg cagaaaaact gctggaacgt ctgcgtctca aaccttatag
    14821 cgatcgttac ccgctgcatc tttctggtgg tcagcagcag cgtgttgcta ttgcccgtgc
    14881 gttgatgatg gaaccgcagg tactgctgtt cgatgaaccg accgccgcac tggacccgga
    14941 aattacggca caaatcgtca gcatcattcg tgagctggca gaaacgaata ttacccaggt
    15001 gatcgtcacc cacgaagttg aagtggcgcg taaaaccgcc agccgagtgg tgtatatgga
    15061 aaatggtcat atcgtagaac aaggcgacgc gagctgcttt accgagccgc aaaccgaagc
    15121 atttaaaaac tatctctctc actaagattc gggataacaa caatgaaaaa agttctgatt
    15181 gccgcgttaa ttgcaggttt tagtctttcc gccacagctg ccgaaaccat tcgttttgct
    15241 accgaagcat cctatcctcc gtttgaatcg attgatgcaa acaaccagat cgttggtttt
    15301 gacgtcgacc tggcacaagc gctgtgtaaa gagattgatg caacctgcac tttctctaac
    15361 caggcgtttg acagcctgat cccaagcctg aaattccgtc gcgtagaagc cgtgatggcg
    15421 ggcatggata tcactccgga gcgtgaaaag caggtgctgt ttaccacccc gtactatgac
    15481 aactctgccc tgtttgtggg tcagcaaggc aaatacacca gtgttgatca gctgaaaggc
    15541 aaaaaagtcg gcgtacagaa cgggacgaca caccagaaat tcattatgga taagcacccg
    15601 gaaatcacta ccgttccgta tgacagctac cagaacgcaa aactggatct gcaaaacggg
    15661 cgtatcgacg gcgtcttcgg tgacaccgca gtggtcactg agtggctgaa agataacccg
    15721 aaactggcgg cggtgggcga caaagtgacc gataaagatt acttcggcac tggcctcggc
    15781 atcgcggtac gtcagggcaa cactgagctg cagcagaaac tcaacactgc gctggaaaaa
    15841 gtgaagaaag atggcactta cgaaaccatc tacaacaaat ggttccagaa gtaattcctg
    15901 atgaatgaat tttttccttt agcaagcgcc gccgggatga ccgtcggcct tgccgtttgt
    15961 gcattgattg tcgggctggc gctggcgatg ttctttgcgg tatgggagtc ggcaaaatgg
    16021 cgtcctgtcg cgtgggcagg ttcagcgctg gtaaccattc tgcgtggcct gccagaaatt
    16081 ctggtggtgc tgtttatcta ttttggctcc tcgcagctgc tgctgacgct ttcggatggc
    16141 ttcactatca atcttgggtt cgtgcagatc ccagtgcaga tggacattga gaacttcgac
    16201 gttagcccgt tcctttgtgg tgtcatcgct ctgtcactgc tgtatgccgc ctatgcctcg
    16261 caaacgcttc ggggcgcgtt gaaagcggtg ccggtgggtc agtgggagtc tggtcaggcg
    16321 ctggggctgt cgaaatcggc tatctttttc cgtctggtga tgccgcagat gtggcgtcat
    16381 gcgctgcctg gcctcggtaa ccagtggctg gtgctgctga aagataccgc gctggtcagt
    16441 ttgattagtg tgaatgattt aatgctgcaa acaaaaagca tcgctactcg tacccaggaa
    16501 ccatttacct ggtacattgt ggcggcggcg atttacctgg tgatcaccct gctcagtcag
    16561 tacattctca aacgcattga cctgcgcgcg acacgttttg agcggaggcc cagctaatgt
    16621 ttgagtattt acccgaactg atgaaagggc tacacaccag cctgacgcta accgttgcct
    16681 cgctgattgt ggcactgatt ctggcattga tttttaccat catcctgacg ctgaaaacgc
    16741 cggtgctggt gtggctggtg cggggttata tcacgctgtt taccggtacg ccgctgctgg
    16801 tgcagatctt cctgatttat tacgggccgg gccagtttcc gacgttgcag gagtatccgg
    16861 cactgtggca tttgttgtca gaaccgtggt tatgtgcgct gattgcgtta tcgctgaaca
    16921 gtgcggcgta taccacgcag ctgttttacg gtgcaattcg tgcgatccca gaaggtcagt
    16981 ggcagtcctg tagcgccctg ggaatgagca aaaaagatac gctggcgctc ctgctgccgt
    17041 atgcctttaa acgctcgctc tcttcttatt ccaacgaagt ggtgctggtg ttcaaaagta
    17101 cctctctggc ttacaccatt acgctgatgg aagtgatggg atacagccag ttgttgtacg
    17161 gacgcaccta cgatgtaatg gtgttcggtg cggcagggat tatttacctg gtcgtcaacg
    17221 gcctgctgac gctgatgatg cgtctgatcg agcgcaaagc actggcattc gaacggcgga
    17281 attaatgtag ctgcgtacat tcgccggggg ggtttgtagg tctgataaga cgcgccagcg
    17341 tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc gtggacgcct tatccggcct
    17401 acatagtgca taaattctta taatgaagac ggacaactca ctaagttatc cgtctttttt
    17461 tatttcattt aaattattta atcatattta ttgcatataa attcacttga tggcattgtt
    17521 atcccatgcc gcagacacgg ccaaaaatca taagattgac agacgggagt tccatcatga
    17581 aaaagttagt tcttgccgct ttacttgctt cctttacttt cggtgcttct gccgcagaga
    17641 aaatcaattt tggcgtttca gccacctatc caccctttga atctataggt gctaataatg
    17701 agattgtcgg ctttgatatc gatctggcaa aagccttgtg caaacaaatg caggcagaat
    17761 gtacttttac taatcacgcg ttcgacagcc tgatcccgtc actgaaattc agaaaatatg
    17821 actccgtaat ctccggtatg gatatcacgc ccgagcgtag caagcaggta tcgtttacca
    17881 cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg
    17941 ccgatctgaa aggcaaacgt attgggatgg aaaacggcac cacgcaccag aaatatattc
    18001 aggatcagca ccccgaagtg aaaactgtct cctatgacag ttatcagaat gcctttatcg
    18061 atctgaaaaa tggtcgtatt gatggggtat ttggtgacac agcggtggta aacgaatggc
    18121 tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg
    18181 gcaccggtct gggcatcgct gtacgtccgg ataacaaagc tctgctggaa aaactgaata
    18241 acgcgctggc agcaattaaa gctgacggta catatcagaa aatcaatgac cagtggttcc
    18301 cgcagtaatt gagactgatg acaaaagtaa gattgcctga tgcgctgcgc ttatctggcc
    18361 cacatcaact ctgcaatgta ttgatttagc atgattttgt aggccggata aggcgtacac
    18421 gccgcatccg gcatgaacaa agcgcacctg atcagcaatc tgaagccgca cctgcggctt
    18481 ttttattgct tcaccagcag cgtcatcact tcatagtgcg cggtgtgcgg gaacatatca
    18541 aaaagctgta cccgttcgat gcggtaacct ggcagttcgc ggatatcttt cgccatggtt
    18601 tgggcgttac agctggagta gataataaaa cgcggtgcca tcgttgagag ataatcacac
    18661 agcggtttac caatgccgcg gcgcggcggg ttaaccagca ccagctccgg cacatccccc
    18721 tgagcggtgg caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt
    18781 tcagcggctg actgctttgc acaggcaatg gcctctgatg caatttcgat cccggttaac
    18841 tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa cagatcccac
    18901 atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg tagcgtacag ctggctggcg
    18961 accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa
    19021 cgctctgcca gcgcctgttg ttcagtcagg tagatctccg tctccccttc cataatcgcc
    19081 atatgtaccg gctgaatatt gacggtaata actttcagtt gcggtagttg ttcctgtaac
    19141 cacggcagcg ccttacgcag ttgtgccagt ttggtttcag aacgcagtac aaagcgcagc
    19201 atcatgccgc catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta
    19261 cgcgccacgt tgtagggcgt taaccccgca cgggcgataa acggttttag cgccgcaaaa
    19321 acgggcgcaa aagaggcagg ataaagcggg cagtcacaaa ggtcttccgg cgtgccatcg
    19381 cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta
    19441 ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaaatcg
    19501 gcgagcagat ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt
    19561 atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct
    19621 tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt
    19681 cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct
    19741 atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat aaccgtaccc
    19801 cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt gtcagcagat atagccagcg
    19861 cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac
    19921 cagactgctt aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc
    19981 gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat
    20041 aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga
    20101 agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcggcatgc
    20161 gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccatgtct tgcacgctgg
    20221 atatcgcgta tccgctgttt ttctgcgcga gccatcagat accaggcgat aaatccgaca
    20281 attccgaccg caccgagaat taacgttgca agggcgttga tttccggatt cactcccatc
    20341 cgcacgctgg aaaagaccag catcggtaac gtggtggctc ccggcccgga aacaaagctg
    20401 gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg
    20461 ggcatgatca tcggtagcgt aatgacgaaa aacactttca gcggcgtcgc accgagatcc
    20521 atcgctgctt cttctatcga gcgatccagt tcccgcaatc ttgaagaaat aacgaccgcc
    20581 acataagccg tacaaaaagt gacatgcgcc agccagatgg tgagcatacc gcggtccgca
    20641 ggccagccaa tggcatgagc aagcgcgacg aataataaca acagcgacaa gcccgtgatg
    20701 ccatctggca tcaccagcgg cgcggtgatc ataaaggcaa aaccatttga tccgcgaaac
    20761 ctgccaaaac gcaccagcac caccgccgca atagtcccga ggatcgccgc cgccgttgcc
    20821 gcacaggcag caattgtcag gcttaaacca accgcactca tcatcgcgtc atcgcgcaat
    20881 aactcaccat accagcgcgt tgaccagccg gcccacaccg tcaccagctt cgagctgtta
    20941 aacgaataga taaccagcat cagcattggc gcgtagagaa aggtaaagcc cagcagcaaa
    21001 atcacaatcc gccagggcga acgaactacc ggtaaattat tcatccgtgt tctcccacgc
    21061 ttttttgctg gtgtttgtgg aaccacatta tcggcacaat tagcagcagc aacatgatga
    21121 tcgctaccgc cgaggccacc ggccagtcgc ggttattgaa aaactcttgc cataacacgc
    21181 gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac tcgcccacag
    21241 ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc ggcacgatca
    21301 cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag cgctgcttcc accagcgaat
    21361 aatcaatacg aatcaacgcg gtataaatcg gcagtaccat aaacggcacg taagcgtaaa
    21421 caatgccgat ataaacggcc agattggtat gcagaatggt cagcggttga tcgataaccc
    21481 ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc atccaggcat
    21541 aaacgcggat cagaaacgag gtccacgacg gcaggatcac cagtagtaat aaaatattac
    21601 gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc agtaaacagc
    21661 aaaatgtcga aatcgccgcc acctgtaacg actggagata agcatcaaaa tagagcggat
    21721 cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat ggaaagttgc ccgtcagccc
    21781 actccatcag ttcggtataa ggtggaatag cgcgcgccat ctctcccagg cttattttaa
    21841 agacaatcag aaatggcagc agaaacagca agatcaacca gatatatagc aacgcaatga
    21901 ccagtttgcg cccatgcttc atttgcagct gcgacaacca cagcttaaat ccgcccggcg
    21961 gtttcgactg ggcagcaggt tcaagtgtac tcatctttgc tccttaaacc gtcagcacca
    22021 cacagctgtc cacttcccag cacaaacgca cttcgtcgcc ccaggtcggt aaccctttac
    22081 gatgacgatg ggcgttttgt agctgggcgc tgatcatctg cccacttttc agtcgaacgt
    22141 gatacaccga aagatcgccg agataggcaa tgtgtatcac ctcccccacc gcgaagttac
    22201 aaccattggc gggcggctct tcgcaaagca tgattttttc cgggcgcagc gctacgtgta
    22261 ccggcacgtt atcgaccact gaagcatccg catcgacttt cagtggatgc accagccccg
    22321 gcgaatcaag caccaggcca tcttcctgac gctctttgag tacaccttca aagacattaa
    22381 ccgaaccgat aaattcagcg ctgtagcggg tagttggatg ctcgtagatc tcttccggct
    22441 cgccaatctg gacaaatttc ccgcgattca taatggcgat acgccccgcc atggtcatcg
    22501 cctcttcctg atcgtgggtg accatcacac aagtcacacc gacgcgctcc agaatatcca
    22561 ccacttcaag ctgcatccgg tcacgcagct ttttatccag cgcgcccatc ggctcatcga
    22621 gcagtaatag tttcgggcgt ttggcaaggc ttcgggccag ggccacacgc tgtcgctgac
    22681 caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg catgtgcacc aggccgagca
    22741 tctcatggac ccgactggca atttccgctt tcggtagttt gtcctgtttc aggccaaaag
    22801 cgatgttctg ttccacggtc atatgcggaa acagcgcgta agactgaaac atcatattga
    22861 tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc atcgagcatt atctgcccgg
    22921 cagaaggttg ttcaaaacct gccagcatac gcagcagcgt ggacttgcca cagccggatg
    22981 cgcccagcag cgcgaagatt tcacctttgt agatggtcag gctgacatca tcgaccgcat
    23041 gctgaccatc gtaggatttg gtgaggttgc ggatttctaa tagcggcgtc agcgccttac
    23101 gggttttcgc ctgcgggcga gggatagcgt cattcacggc tgctctccgg cattgaatgt
    23161 gtgcaagcgc accatgatgg tgcgaattgc cggctggtgt ggtgcgcccg cccggcatct
    23221 aaggctgcgg attattttcc gctcttcact ttggtccacg cgcgggtgcg cacacggtcg
    23281 attttcggat cctgtacttt cagagtgaat agcttcgcac gaacgtccgc cggaggataa
    23341 atacccgggt tctcacggac ttccgcactc accagcggcg tggctgcttt attggcgttg
    23401 gcatagaaca catggtcgga aatatgcgct actacatccg ggcgcagcag gtaattcagg
    23461 aactgatagg cttcgtcttt gtttttggca tccgcaggca tggcgaatac atcaaagaac
    23521 gccatcgccc cttcttttgg aatcgagaac gagacattca ctccattctt cgcttccttc
    23581 gcgcggtttg acgcctgcca gacatcacct gcccagccga tagcgacgca aatatcgccg
    23641 tttgccaggt cgttaatgta ttgagatgaa tggaaataac gaatgttcgg gcgcagcttt
    23701 aacagcagat cggttgccgg tccggtgtaa tcatccgctt tagtgctgtt gggatctttg
    23761 ccgagataat tcaacacggt agcaaaaact tcttctggcg catccaggaa agagacaccg
    23821 cagcttttca gtttttccag attttcaggt ttgaggatca agtcccagct atcgacgggc
    23881 gcgttttcgc ccagcacagc tttaacttta tcaacgttat agccaatccc ggtcgtcgcc
    23941 cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt tggcgaccag cttcagcagc
    24001 tccgggtcga gattcttcca ctccggcaat ttgcttttgt ccagcggctg gaaaactccc
    24061 gcagtcaact ggcgttcaag aaagctggca gatggaacca ccaagtcaaa gccggtgcta
    24121 cccgccatta atttgccttc cagtacttcg ttagagtcga aaacatcgta gacgacttta
    24181 ataccggttt ctttttcaaa attggccacc gtgtccgggg cgatataatc agaccagtta
    24241 taaatgtgga gtgttttttg ttcagccgcg agcgtgccga cagagacggc catcagagca
    24301 cccgcagcca gacccgaaag ccatttttta tttaaggcgg tcatatctct ttccttctga
    24361 aagttcgtta acaaacgaac aaaagttgtc acactaaaag atatgcaata aacgtgcata
    24421 ttttttatcg gcggggtgta agaaaagaga actctctccc ggccaacact gctcgctata
    24481 taaaagcatc gcaagaataa ctttagccag ggtttgagac tatagctcgg attaaaaaac
    24541 ggtatttaac aaatttttat gcgtatttgc tctatgtgat aaagccaaat cgacacaaga
    24601 gaagtgaaat aatctttaag aaaaggttaa aaacagcaga taattttgca gcgcgcagcc
    24661 actgcaccag cgactgcgtg ctcgaaagga gttatcagtg gaggaaatag ttttcggtaa
    24721 cgttgttttg cccttcatcg tcagtcggta acagcagttg atgggcatta gcttcaagaa
    24781 tgaccatcga aatctgctct tcgctctgac gcacgaacca tgcaaactgc tcataggtta
    24841 cgccctgcat aacggataaa gactggcaaa ccaccagctt tggcagatta tcatcctgca
    24901 tatcaagaaa tgctttcacg gtcagcgaac tggcattgat ggcagataaa tccgccgcca
    24961 gcggcaatac ggctgaaggt cggacttccg ccatggcaga aaacaggatc gtgttatcga
    25021 tcagatcgat tttggcatca aagacaccgt cgaaattctg catatggggc agatgcagag
    25081 cctgacagtt atcacattca aaaaaactca tccccaggtc atcgagccat tgacgcagcg
    25141 tatccagacc aggaacgacc agcgatgtca taattttgta caacctcttt cgataaaaag
    25201 accggcacag cttacgcaaa aagcgcaggc aaaaccatga tcagtaatgt gattgcgact
    25261 aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg ttcgatccag cggatcattt
    25321 tacccgcgat gtcgatacca gtggtttttt ctattccttc cagccccggc gacgcattca
    25381 cttccatcac caacggcccg cgattagcac gcagaatatc cacaccagca acgtccagcg
    25441 ccatcgttcg cgcggctttt atcgcgattt cacgctcctg tggtgtgata ctggcgacgc
    25501 ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc ctctttcgcc cgccgttcaa
    25561 tcgcagcgac cacttcatcg ccaacaacca gacagcggat atcgcaccct tgcgcctctt
    25621 tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg gaaagcgtca atcacgcttt
    25681 ccgccgcctg acgcgtctcc gccagcacga cgccaattcc ctgcgtgcct tcaaccaact
    25741 tgaccaccag cggcgcacca ccgaccatgt cgattaaatc gctggtatca tccggcgaat
    25801 gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc cagcagttgc atggaacgca
    25861 atttgtcacg cgcccgggca atggcgaccg actcattgag cggatagctc cccagcatct
    25921 cgaactggcg cagtgccgcc gtcccataaa aggtaatggc ggtgccaata cgcgggatca
    25981 ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat agaagacgcc gcaggattta
    26041 tgttcatgta gcaagaaagc ggatcaagaa tttcaaccag gtgaccgcgc tgtatagcgg
    26101 cttcacgcag ccgcttacac gaatagagcg ttccatcccg ggacaatatg gcaattttca
    26161 ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta tcatacatcg acgtggcggt
    26221 tttagcgcgt cgcccaaccc tgtttgtgca aataatccag aataaatggg cggctttctt
    26281 taatgattgt tcggcggata tgatcgctcc aggtatcccg gcgattattg ctgccacggg
    26341 tgaggtaata ttccgccagt tgctcgtcat actgcgccag tgcgtcttta tccagcggtt
    26401 gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg cggcttgaga tccggattat
    26461 ccgcaggcca gccaaggcac agcccaaaca gcggcagaac atgctgcggt aatttcagca
    26521 gtttcgtcac cgcttcaata ttattgcgca ggccgccgat atataccccg
//
LOCUS       DAJVXP010000047        26336 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVXP010000047 DAJVXP010000000
VERSION     DAJVXP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.047"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..868)
                     /locus_tag="OQS10_001883"
     CDS             complement(<1..868)
                     /locus_tag="OQS10_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2292919.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVA"
     gene            complement(928..1479)
                     /locus_tag="OQS10_001884"
     CDS             complement(928..1479)
                     /locus_tag="OQS10_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS2292920.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            complement(1762..2052)
                     /gene="ubiK"
                     /locus_tag="OQS10_001885"
     CDS             complement(1762..2052)
                     /gene="ubiK"
                     /locus_tag="OQS10_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS2292921.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      2174..2322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2426..3079
                     /gene="ribB"
                     /locus_tag="OQS10_001886"
     CDS             2426..3079
                     /gene="ribB"
                     /locus_tag="OQS10_001886"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS2292922.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            3340..3510
                     /locus_tag="OQS10_001887"
     CDS             3340..3510
                     /locus_tag="OQS10_001887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292923.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(3568..4341)
                     /gene="zupT"
                     /locus_tag="OQS10_001888"
     CDS             complement(3568..4341)
                     /gene="zupT"
                     /locus_tag="OQS10_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS2292924.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            4484..5272
                     /gene="ygiD"
                     /locus_tag="OQS10_001889"
     CDS             4484..5272
                     /gene="ygiD"
                     /locus_tag="OQS10_001889"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS2292925.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(5310..6470)
                     /locus_tag="OQS10_001890"
     CDS             complement(5310..6470)
                     /locus_tag="OQS10_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS2292926.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(6476..7147)
                     /locus_tag="OQS10_001891"
     CDS             complement(6476..7147)
                     /locus_tag="OQS10_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS2292927.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(7295..8776)
                     /gene="tolC"
                     /locus_tag="OQS10_001892"
     CDS             complement(7295..8776)
                     /gene="tolC"
                     /locus_tag="OQS10_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS2292928.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8981..9610
                     /gene="nudF"
                     /locus_tag="OQS10_001893"
     CDS             8981..9610
                     /gene="nudF"
                     /locus_tag="OQS10_001893"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS2292929.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            9611..10033
                     /locus_tag="OQS10_001894"
     CDS             9611..10033
                     /locus_tag="OQS10_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS2292930.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            10058..10885
                     /gene="cpdA"
                     /locus_tag="OQS10_001895"
     CDS             10058..10885
                     /gene="cpdA"
                     /locus_tag="OQS10_001895"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS2292931.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            10885..11466
                     /gene="yqiA"
                     /locus_tag="OQS10_001896"
     CDS             10885..11466
                     /gene="yqiA"
                     /locus_tag="OQS10_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS2292932.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            11495..13387
                     /gene="parE"
                     /locus_tag="OQS10_001897"
     CDS             11495..13387
                     /gene="parE"
                     /locus_tag="OQS10_001897"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS2292933.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13435..13749)
                     /locus_tag="OQS10_001898"
     CDS             complement(13435..13749)
                     /locus_tag="OQS10_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS2292934.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(13780..14361)
                     /gene="mdaB"
                     /locus_tag="OQS10_001899"
     CDS             complement(13780..14361)
                     /gene="mdaB"
                     /locus_tag="OQS10_001899"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS2292935.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(14471..15820)
                     /gene="qseC"
                     /locus_tag="OQS10_001900"
     CDS             complement(14471..15820)
                     /gene="qseC"
                     /locus_tag="OQS10_001900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS2292936.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(15817..16476)
                     /gene="qseB"
                     /locus_tag="OQS10_001901"
     CDS             complement(15817..16476)
                     /gene="qseB"
                     /locus_tag="OQS10_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS2292937.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            16628..17020
                     /gene="ygiW"
                     /locus_tag="OQS10_001902"
     CDS             16628..17020
                     /gene="ygiW"
                     /locus_tag="OQS10_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS2292938.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            17073..17555
                     /locus_tag="OQS10_001903"
     CDS             17073..17555
                     /locus_tag="OQS10_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2292939.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            18101..20359
                     /gene="parC"
                     /locus_tag="OQS10_001904"
     CDS             18101..20359
                     /gene="parC"
                     /locus_tag="OQS10_001904"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS2292940.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            20592..21329
                     /gene="plsC"
                     /locus_tag="OQS10_001905"
                     /gene_synonym="parF"
     CDS             20592..21329
                     /gene="plsC"
                     /locus_tag="OQS10_001905"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS2292941.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            21404..22816
                     /gene="ftsP"
                     /locus_tag="OQS10_001906"
     CDS             21404..22816
                     /gene="ftsP"
                     /locus_tag="OQS10_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS2292942.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            22927..25146
                     /locus_tag="OQS10_001907"
     CDS             22927..25146
                     /locus_tag="OQS10_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS2292943.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINHYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(25189..25446)
                     /gene="yqhH"
                     /locus_tag="OQS10_001908"
     CDS             complement(25189..25446)
                     /gene="yqhH"
                     /locus_tag="OQS10_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS2292944.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(25497..>26336)
                     /locus_tag="OQS10_001909"
     CDS             complement(25497..>26336)
                     /locus_tag="OQS10_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS2292945.1"
                     /translation="RQIYQNYKSDASTPYFGETGERAITSARIQQALTLNDNLTLPGN
                     IGWLDYDPVCDCQDFGDLVLESVAITQTDADHADAVVRFRIFKDDKEKTTQTLKMVAE
                     NGRWVIDDIVSNHGSVLQAVNSENEKTLAALASLQKEQPEAFVAELFEHIADYSWPWT
                     WVVSDSYRQAVNAFYKTTFKTANNPDEDMQIERQFIYDNPICFGEESLFSRVDEIRVL
                     EKTADSARIHVRFTLTNGNNEEQELVLQRREGKWEIADFIRPNSGSLLKQIEAKTAAR
                     LKQ"
BASE COUNT         6256 a         6524 c         6934 g         6622 t
ORIGIN      
        1 gtgcaacacc cgtgatttgc ggtgcgtaac cacgtagatc aggcggcaac atattctcat
       61 cgcttttcaa cgaaacgcca gaaaaatgga aagagtcgaa aatagaggat tcagtatcgt
      121 attgacctat agttagtttt gcattaattg ctgggattgg gcgaaacaaa taaatacgcg
      181 gaaaatccag gttgttcgcc tggcttttgc cattgttaaa tgactgatcg tactggtaat
      241 cagtacgcag gcgccaggcc ccaagattaa ccccagcctg cccataggaa cttatatttt
      301 gtgaattatc gccctgatgt ggtgcataat gagaggcata taaattgtag tcaagaaatg
      361 ccccagcaat accatgatcc caaagttcag gaggcgtcca gtttgcatct ttgtatttta
      421 accatgcctg aggtgcagat atggataatt gcattttccc tttatcgaga taagttgtaa
      481 tttgtttttc tttttcgata gggtaacaac catcgatcag gttttgctga gcctcttcaa
      541 taatctcatc agtaaagcct aataatgtta attgctctgg agtcagacaa actgcagttt
      601 ggttatcaat taatacccat tgcaggtcca gatggattgg cgtcctttta ttattaataa
      661 gagcgtttaa tgagtattta cctacgggta attggccatc agtttcaaat tgggaaaggt
      721 taacgttttc acgatcttcg gcttcgatta aatctttgtt gaattcaacg gcataagctg
      781 acgcacaacc aatgatgaga acggataatg aacaataaat atttttaatc aattcgctta
      841 ttgtggttaa ttttaatttt ttatacatct gttccactta tcattaatcc gttaatgatg
      901 tggccgggcg aacccggcct aatgtgatta ctcgtaagca atcttatagt cagcctgagc
      961 gtaaacgttg cccggggtag catctttgcc cggggattcc atacgagctt tgaagttcag
     1021 ttcctgatag ctgttagtat tgttgaatgt aattactacc ggagtaccta aagtcacttc
     1081 gccgctctgg ctatccagaa tacgtacgcc aacgcctgtg gcaccaccga tttcagtttc
     1141 tttggtgttt ttcagcaggt cagtctgacc cgtcatagta ttcgctgtgg tgaaagtaac
     1201 cttcgctttt gaataagtgg ttccaccctg gctcaacatg cagtcttgca ggtgaatagt
     1261 gaaaggctca gccaaagatt tctggtcgct tttcagaacg gtatctgcta cttcacccag
     1321 atttacagtt aagtcttcgt cacctggttt aattgaacat ggagcagaga taacttcacc
     1381 tttaaattta acaatgccgt gaccttcgtc agcaaatacg ttattggaaa gaatcattgc
     1441 tacgccagca acaagaagtg actttttaaa agcagacata atattccttt atataaatat
     1501 aagtagaaaa tgaatgatta gtaactgtta tttaaggatt gctaatcatt caaatattat
     1561 attttacttt tcgggtttta tgaaattagt ttatctaaat atggattaag taaaacttaa
     1621 aaaatacata acaattatat tttcaattgc actcattcat ttttgtgatg atattctgaa
     1681 tggaattaag cgataaggta atagaaagca ctgtaaatta gaagattaat tcataattta
     1741 cagtgcatta aagacaaatg tttacagcgt tggggggaga gtctctggat ctggctgctt
     1801 tttaatctca gtactgcgat tttccagttc gctcattctt tgttcaagcc gcgccagttt
     1861 ctcacgagtc cgtaacagga cttgcgtttg gacgtcgaac tcttcacggc ttacaaggtc
     1921 gaggcgcgtc agctgagctt gtagggtttg gcggattttt ttctccacat cttccccgaa
     1981 ctccctgatt cctttaggca ttgattcgtg aacctggcga gcgatttgct caattttttt
     2041 cgggtcaatc attgtagttt ccctgtactg ataggtgttg agtaccattg tagtgcgata
     2101 agggtaagtc ataaaccaga attatgtgaa gctatgcgtt gctgccgcta atcattagcg
     2161 ttatagtgaa tccgcttatt ctcagggcgg ggcgaaattc cccaccggcg gtaaatcaac
     2221 tcagttgaaa gcccgcgagc gctttgggtg cgaactcaaa ggacagcaga tccggtgtaa
     2281 ttccggggcc gacggttaga gtccggatgg gagagagtaa cgattctgtc gggcatggac
     2341 ccgctcacgt tattttggct atatgccgcc actcctaaga ctgccctgat tctggtaacc
     2401 ataattttag tgaggttttt ttaccatgaa tcagacgcta ctttcctctt ttggtacgcc
     2461 tttcgaacgt gttgaaaatg cactggctgc gctgcgtgaa ggacgcggtg taatggtgct
     2521 tgatgatgaa gaccgtgaaa acgaaggcga tatgatcttc ccggcagaaa ccatgaccgt
     2581 tgagcagatg gcgctgacca ttcgccacgg tagcggtatt gtttgcctgt gcatcactga
     2641 agatcgccgt aaacaacttg atctgccaat gatggtagaa aataacacca gcgcctatgg
     2701 caccggtttt accgtgacca ttgaagcggc tgaaggtgtg actaccggtg tttctgccgc
     2761 tgatcgtatt acgaccgttc gcgcagcgat tgccgatggc gcaaaacctt ctgatctgaa
     2821 tcgcccgggc cacgttttcc cacttcgcgc gcaggcaggt ggtgtactga cgcgtggcgg
     2881 tcatactgaa gcgactattg atttgatgac gctggcaggc tttaaaccgg ctggtgtgct
     2941 gtgtgagctg actaatgacg atggcacgat ggcgcgtgca ccagagtgca ttgagtttgc
     3001 caataaacac aatatggcgc tcgtgactat tgaagacctg gtggcatacc gtcaggcaca
     3061 tgagcgtaaa gccagctgaa aaccgctgct taattactgc cttaatcaag aaaccgaagt
     3121 tgtagctggc ttcggttttt attttgccct gctatgcaag atgttaattc cgcttctctt
     3181 tctatgagaa aatttcatta atatcaggca ttctttttca ttatgatcaa aatcatataa
     3241 ttgttccgtt attatgcgac catatgaata tatccgaaaa aggatatgtg aaatttatgc
     3301 gctaaatgtt tagtattgtt aaatattaag ggtaaaagga tgtttatcgc ctggtactgg
     3361 attgtattga ttgctctggt tgtggtgggt tatttcctgc atttgaaacg ttattgtcgg
     3421 gcgtttcgcc aggacagaga cgcactgctt gaagcgcgga acaaatactt aaacagtacg
     3481 agagaagaga cagccgaaaa ggtagaataa ggctttcgtg aagctggcag aagttgtcca
     3541 tgtgctggac aacttctgat gttgttttta accaattccc gccgtttgta gcagcactaa
     3601 actgaatccc atcactgaca ttccacatag tacgccatag ctggggttat tattagggtc
     3661 aatctctttg gcgagcggca ttaattcatc aaccgagagc gccaccataa ttcctgcaac
     3721 cgccgccatg attgccgcca tgaccaccgg ggaaatcatg ctaccgagga ttaaccacgc
     3781 cagcacacca ccaagaattt ctgctagtcc agaaatcccc gcccacagaa tcgcggtgcg
     3841 tttagacccc gttgccgcat aaaccgggcc tgccactgcc agaccttcag ggatattgtg
     3901 caacgcgacg gccagtgcga tgccaaatcc cagctccagg ttgctgctcg ccgtgacaaa
     3961 ggtggcaatc ccttccggga agttatgcag actgatgccg agagtgagca gaatggctgt
     4021 gcgcttgatc gattttggca acggctgcac cgatttttgc attaaatcct gcggatgcgc
     4081 atgtggcaac atgcggtcca ggccaaaata gccaagcaga ccgaagataa acattccata
     4141 acccaacaca ggcgacattc cttcagctgc tagtgcggca ggaagcattt ccattaatga
     4201 gatgagcaac atgatccccg ccgcaaatcc tagcgaaaac gccagtaagc ggttcgaggg
     4261 tttttgcccg agaacgccga gaaacgcgcc aataaacgtg gctgcccccg ccagtatggt
     4321 cagaatgaga ggtactgaca tcgacaactc cttataaatc ttcaccttca ggcagatcgt
     4381 caaaataact gatgatcatc atcattgtta tccgggttct tactccgccg aacaagcgta
     4441 ttgtgaggat atcaaaatga cacctttagt taaggatatc atcatgtctt caacacgtat
     4501 gccagcattg tttttaggtc acggtagtcc gatgaacgtg ctggaagata atttgtatac
     4561 ccgcagctgg cagaagttgg ggatgacatt gccacgcccg caagcgattg tggtggtttc
     4621 ggctcactgg tttacccgtg gaacaggagt gaccgcgatg gagacgccgc ccacgattca
     4681 tgactttggt ggcttcccgc aggcgctgta cgatacgcat tatcctgctc cgggttcgcc
     4741 tgtgctggca cagcatctgg ttgagctgtt agcgtcggtc ccggtgacgc tggataaaga
     4801 agcctggggc tttgaccacg gttcctgggg cgtgctgatt aagatgtatc ctgacgccga
     4861 tatcccgatg gtgcagttga gtatcgacag tagcaaacct gccgcctggc atttcgaaat
     4921 ggggcgcaaa ctggcagcgc tgcgagatga aggaataatg ttggtcgcca gtggcaacgt
     4981 ggtgcataac ctgcgtacag tgaagtggca cggtgatagt tcaccatatc cttgggcgat
     5041 gtcgtttaat gagtatgtga aagcgaatct gacgtggcaa ggggcggtag agcagcatcc
     5101 tctggtgaat taccttgacc atgaaggtgg cgcgttatcg aacccaacgc cggagcacta
     5161 tctgccgttg ttgtatgtct taggtgcgtg ggatgggcag gagccaatta ccattccggt
     5221 cgatggtata gaaatgggca gcctgagtat gctgtcggtg cagataggtt aacaattacc
     5281 ggatggcgaa tgccatccgg catcattgat tactcaataa aaatatgtgg ataaaaccga
     5341 gacatatcct gggtgatcaa cgcacggtct tcacgaatgc caattccggc aggttgatcg
     5401 ttcaccagcc agctaccaat cagcatatag ctgtcgccga atttcggtaa cgggtggaat
     5461 tgctgaacaa tcatcccttc ttcgccatac ggaccttccg ctgcttcaat ggttttgccg
     5521 ttctcaatga tcgacacgtt tgcgccttca cgggagaaga tcggtttaac cacatatttt
     5581 tccatttgcg gatgatcatc ttccgcaaaa taagcgggca gcaggttcgg gtgattcggg
     5641 aacatctccc atagtagcgg tagaagcgct ttgttggaga taatgctctt ccacgccggt
     5701 tccagccagc gtacgcctgc atcctccagc ttggtggaga acatctcacg caacataaat
     5761 tcccatggat acagcttgaa caggttggaa attacctgat cctgtaaatc cgtgaactga
     5821 cctttttcac ctaacccgat atcgtcaatg tagaggaact cggtagcgat ttcagcttcc
     5881 gtcgcgcagt cctgcaaata ctgaatggtt ccgcgatctt ccaccgtgtc gcgacagcag
     5941 gtgagatgca gcaactggaa gccatactgt tcacgcaact cgacaaagcg atcgattagc
     6001 ttttcttgca gactgttaaa ctggtcgctg ccctccggca agttaccggc gttaagctga
     6061 tcttccagcc agatccactg aaagaacgcc gcctcgtata gtgacgttgg tgtatcggcg
     6121 ttattttcca gcagtttagg ttcgccagtg ccatcccatg ccagatcaag acgcgaataa
     6181 agcgatggct ggtgagtcag ccatgactga cgcacaaaac tccaggtgcg ttttggaatg
     6241 cggaatttgg tcatcagctc atcgctggcg atcacttttt ccaccacttt caggcacatc
     6301 tggtgcagtt cggcggtgac ttcttccagc ttttcaacct gggcgagggt caacttgtag
     6361 taagcatctt cacaccagta cggctcgccg tacatggtgt gaaaattgaa accgtattcg
     6421 tgggcttttt cgcgccagtc cgggcgctcg gtaatactga ctctttccat cggtatcagc
     6481 cacccattga acgagaagag gtgccggttg cgctacgctg catagtgctt tgtttggcaa
     6541 cagattcacc aaaaccgcca cgggtaacgg tagtagtggt cgccggtttt ggtgccatgg
     6601 ctgtcttcgg tacggtcatg gtgcggcctg gctgggctgc gccatagttt ttacccgtcg
     6661 cgtcggtata tttaccgtaa gccgggctgg ctgggttttt cgaggagaac agcggctgct
     6721 gtgcaaatcc cgcgccgccg cccatcagac gccccatcat gtaaccggcc atcagcggca
     6781 tccagaaact cccgctggat tgctgggcct gcgcctggtt ttctggtgcc atgccagcct
     6841 gggctggtgc ctgctggcac tgaccttcac caaattcagc aacacagtct tcacgggtgg
     6901 cgtatttcgg cgcagtacgt tcggcttctt tcagcgcatt gttgtacgcg gtggtacatt
     6961 cggcgctttt gcctgggttt gcagctgaac agtcgtcagc attttgatag agagacactg
     7021 tttcatcact cttttcacag ccagccagca taaaaacagt ggcaaccgcg agagcgactg
     7081 gtgtcagatg gcgtgcgctc cagtttttgc ggaacgatgc gtggcgtatg gattttgtcc
     7141 gtttcatttt tgtcttccgg gaccagtggt aaatacccat cagaatagag gatggctggt
     7201 cgaaattgaa gcgagaaggg ggaagaatgc ggcagataac ccgtatcttt acgttgcctt
     7261 acgttcagac ggggccgaag ccccgtcgtc gtcgtcagtt acggaaaggg ttatgaccgt
     7321 tactggtggt agtgcgtgcg gatgtttgct gaacgactgg tgccgggcta tcaggcgcat
     7381 aaccatcagc aatagcattc tgttccggcg tttgcggtgc aacgttttcc ggattagtgg
     7441 aaaccggttt gctcagcgca ttgttcagtg ccagcagatc ctgctcgttc aacgtaccca
     7501 gagctgactt aatattcaac tggttaatca ggtagttata acgcgcattt gccagctctt
     7561 gcttagcgtt gtacagcgtg gtggttgcat ccaacacatc aacaatggta cgcgtaccga
     7621 ccgagtagcc cgcttccatc gcgtctaatg agctttgagc ggaaactacg gcttgtttgt
     7681 aggcgttaat gctactgata gatgcattaa tgttgttgaa ggaggaacgt acggtttgca
     7741 cgacgctacg atgcgcgctt tccagttgct cgctggcacc aacaaagttg tactgggcct
     7801 gtttcacctg cgagttaacc attccgccct gataaatcgg cagcgagaag ctcaggccca
     7861 ctttgttctg gcccatattg ctgtcgtcat actgggtacc agcggcacca cgggttttcg
     7921 aaccgctata agaggtgtca gaaatcccgg tagaagccgt taaatccagc gtcggtaagt
     7981 gaccatcctg cgcctggcga atttgctcgc gcgccaggtc ctggctcaag cgtgcctgta
     8041 acagcgacag gttgcgtttt tcggcttctt tcagcagcgc gttaaccggc tgtggtttgt
     8101 cggttttaaa gttttcgaca ttcagcgccg ccagttccgg atagtagtta ccggtgatct
     8161 ggcgcagctg ctctaccgcg ttatcaaggt tattacgtgc ggtcacttcg ttcgccagca
     8221 cggtatcgta ctgcgcgcgg gcgttctgca cgtcggtgat cgctaccagg cccacgttaa
     8281 aacgttgggt ggtttgatct aattgacggt agatcgcttc tttttgtgcc tgtgtatagg
     8341 aaagaacgtc aatagcattc aacacgttga aataagcggt cgcggtgttg aggatcaagg
     8401 tttgctgatc ggtctgatat gtgacgtcct gaatccctgc tgctttttcc tgcagcgtta
     8461 acgcacgcca tttcgacata tcaaaaatgg attgagttaa ctgcagggac gcactggtcg
     8521 cgttagagtt gatgccgttc gcgtcgcggt agccgttgct ataggtgtaa tctgcaccta
     8581 aacctagctg tggcagtaat ggactgcgcg cttcattaat tttttcaaag gcagcatcac
     8641 gatcggcggc agacttacgc aattccgggt tactaaggcg tgcttgctga taaacttgca
     8701 tcaggttctc ggcctggctc aacgaactga acccagaaag gctcaggccg ataagaatgg
     8761 ggagcaattt cttcatttgc attccttgtg gtgaagcagt atttagcgcg atcaaactgt
     8821 aaaattattg cggattctag cagaagccgc taccgcaaaa ggttggcgtt acgtgccata
     8881 gggcgttaat gtgcctcaat ttaacacatg actgctcaaa cggcagtcaa acgtcgctga
     8941 aattcacatt taattcacta ttagtgccag gacattaaca atgcttaagc cagacaacct
     9001 gcccgttaca tttggcaaaa acgatgtaga aattattgca cgagaaacac tttatcgcgg
     9061 ctttttttca ttagatcttt atagatttcg tcatcgtcta ttcaacgggc aaatgagtca
     9121 tgaggtacgg cgggaaattt ttgagcgcgg tcacgccgca gtcttgctac cctttgaccc
     9181 agtgcgcgat gaagttgtgc tgattgagca gattcggatt gccgcatacg acaccagtga
     9241 aaccccctgg ctactggaga tggttgccgg gatgatagaa gagggtgaaa gtgtggaaga
     9301 tgttgcccgt cgcgaagcga ttgaagaggc gggactgata gtcaaacgga ccaaaccggt
     9361 gttaagtttc ctggcaagcc cggggggcac cagtgagcgt tcgtcaatta tggtgggcga
     9421 agtggacgcc acgaccgcaa gcggtattca tggtctggct gatgaaaacg aagatattcg
     9481 cgttcatgtg gtaagccggg aacaggcata ccagtgggta gaagagggga aaatcgacaa
     9541 cgcagcgtcg gtcatcgctt tgcaatggct gcagctgcat catcaagcgt taaaaaatga
     9601 gtgggcataa atgaagcgtt acacacctga ctttcctgaa atgatgcgcc tgtgcgagat
     9661 gaacttttca caattgcgcc gtttgttacc gcgcaatgac gcacccggcg aaactgtaag
     9721 ctatcaggtg gcaaacgcac aatatcggct gacgattgtg gaatcgaccc gatacactac
     9781 cctggtgaca atagaacaga ctgcgcccgc gatcagttac tggagccttc cgtcaatgac
     9841 ggtgcgtctg tatcatgacg cgatggtggc tgaagtgtgt tcaagccagc agatttttcg
     9901 cttcaaagcg cggtatgatt atcctaataa aaagttgcat caacgcgacg aaaagcatca
     9961 aattaatcag tttttagcgg actggttgcg atactgttta gcacatggag cgatggcgat
    10021 tccggtttac tagcatcgtg aaacctaagg acaccatttg gaaagcctgt taacccttcc
    10081 tctggctggt gaggccagag tcaggatttt acaaattacc gacactcacc tgtttgcaca
    10141 aaagcacgaa gctctgttag gggtaaacac ctgggaaagc taccaggcgg tgctggaggc
    10201 gattcgtcca catcagcacg aattcgacct gattgtcgcg acaggtgatt tagcgcagga
    10261 tcaatcctct gcggcctatc agcatttcgc tgaaggcatc gcaagttttc gtgcgccctg
    10321 cgtctggctg ccgggcaacc acgatttcca gcccgcgatg tacagcgcgt tacaggatgc
    10381 gggtatctcc ccggcgaagc gggtgtttat tggtgagcaa tggcaaatcc tgttgctgga
    10441 tagccaggtg tttggcgtgc cgcacggtga gctgagcgag tttcagcttg agtggctgga
    10501 acgtaaactg gccgatgcgc cagaacgcca tacgttgctg ctgctgcatc atcatccgct
    10561 acctgcgggt tgtagttggc tcgatcaaca cagtctgcgt aacgcgggcg aactggatac
    10621 cgtgctggcg aagtttccgc acgtcaaata cttgctgtgc ggtcatattc atcaggagct
    10681 ggatctcgac tggaacggtc gccgcctgct ggcaacgccg tcgacctgtg tgcagtttaa
    10741 gccgcactgt tccaacttta cgctggatac tatcgcgccc ggctggcgta ctctcgagtt
    10801 acatgctgat ggcacgctga ccaccgaggt gcatcgcctg gcggacacac gtttccaacc
    10861 tgataccgct tcagaaggct actgatgtct acgcttcttt atttacacgg tttcaacagc
    10921 tcgccgcgct ctgcaaaagc gagcttgtta aaaaactggc tggcggaaca tcaccctgac
    10981 gttgagatga tcattccgca gttgccgccg tatccttccg acgcggcaga actgctggaa
    11041 tccattgtcc tggaacatgg cggcgattcg ctgggtattg tcggttcgtc actgggggga
    11101 tattacgcca cctggttgtc acaatgtttt atgctgcccg cagtggtggt aaacccggcg
    11161 gtgcgcccgt ttgaactgct gacggactat ctcggtcaga acgagaaccc atacaccggg
    11221 cagcaatatg tgctagagtc acgccatatt tacgatctca aagtcatgca gattgacccg
    11281 ctggaagcgc cggatttgat ctggctgctg caacagacgg gagatgaagt gctggattac
    11341 cgccaggcgg tggcgtacta cgcttcctgc cgccagactg tcatagaagg cggcaaccac
    11401 gcattcacgg gcttcgaaga ttatttcaac ccgatcgtcg attttcttgg tctgcaccat
    11461 ctctgacgat caactcgaat tgctaactta aaccatgacg caaacttata acgctgatgc
    11521 cattgaggta ctcaccgggc ttgagccggt tcgccgccgt ccggggatgt ataccgatac
    11581 cactcgccct aaccatttgg ggcaagaagt tattgataac agtgtcgatg aagcactggc
    11641 gggtcacgca aaacgcgtgg atgtaatctt acatgctgac cagtcgttag aagttattga
    11701 cgatgggcgc gggatgccgg tggatattca cccggaagag ggtgtcccgg cggttgaatt
    11761 gattctttgc cgtctgcatg caggcggtaa attctctaac aaaaattacc agttctctgg
    11821 cggcctgcat ggcgtgggga tttcggtggt taacgccctg tcgaagcgcg ttgaagttaa
    11881 cgtgcgccgc gatggtcagg tttataacat cgcctttgaa aatggcgaaa aggtgcagga
    11941 tttacaggtt gtcggcactt gcggtaaacg caataccggt accagcgtgc acttctggcc
    12001 ggatgaaacc ttctttgaca gcccgcgatt ttctgtttca cgcctgacgc atgtgctgaa
    12061 agccaaagcg gtactgtgcc ccggcgttga gattaccttt aaagatgaga tcaacaacac
    12121 cgaacagcgc tggtgctatc aggacggtct gaatgattac ctggcggaag cggtaaacgg
    12181 tttaccgacg ctgccagaaa aaccgtttat cggtaatttc gctggcgata ctgaagctgt
    12241 ggactgggcg ctactgtggc tgccggaagg cggtgaactg ctgaccgaaa gctacgtcaa
    12301 cctgatccca acgatgcagg gcggtaccca tgttaatggc ctgcgtcagg gcctgttgga
    12361 cgcgatgcgt gagttctgtg aatatcgcaa cattctgccg cgcggcgtga agctgtcggc
    12421 ggaagatatc tgggatcgct gcgcctatgt tctgtcggtg aaaatgcagg atccgcagtt
    12481 tgccgggcag acgaaagagc gtctctcttc gcgtcaatgc gcggcattcg tttctggcgt
    12541 ggtgaaagat gcctttatcc tgtggctgaa ccagaacgtt caggcggcgg agttgctggc
    12601 agagatggcg atttccagcg cccagcgtcg tatgcgtgcg gctaaaaaag tggtgcgtaa
    12661 aaagctgacc agcggcccgg cgctgcctgg caaactggct gattgtaccg cgcaggacct
    12721 taaccgtacc gaactgttcc tcgtggaagg tgactccgca ggcggatctg ccaagcaggc
    12781 gcgcgatcgt gaatatcagg cgatcatgcc gctgaaaggt aagatcctta acacctggga
    12841 agtttcttcc gacgaagtgc tggcctcgca ggaagtgcat gatatttcgg tagcgatcgg
    12901 tatcgatcct aacagcgacg atctgagcca gcttcgttat ggcaaaatct gtatcctcgc
    12961 ggatgcggac tctgatggtc tgcacattgc cacgctgctc tgcgctttgt tcgtaaaaca
    13021 cttccgcgcg ttggtgaaac acggtcacgt ttacgtcgca ctgccaccgc tctaccgtat
    13081 tgatctcggg aaagaggttt attacgcgct gacggaagaa gagaaagagg gcgtacttga
    13141 gcaattaaaa cgcaagaaag gcaagccgaa cgtccagcgt tttaaaggtc tgggggaaat
    13201 gaacccgatg caattgcgtg aaaccacgct tgatccgaac actcgccgtc tggtgcagtt
    13261 gactatcgat gatgaagacg atcagcgtac tgacgcgatg atggatatgc tgctggcgaa
    13321 gaaacgctcg gaagatcgcc gcaactggtt gcaagagaaa ggcgacatgg cggagattga
    13381 ggtttaaagg aaagaacatt gcccggtcag gatggccggg caaacaaggc aggattaaat
    13441 ccctggctgc agaatacgaa tattcatctc cagcacgtcg ccttttacgg cttcgctgta
    13501 cgccttcatg tgcggggttt gcagatgcgc ttcaaggtgc gcgatgcttt cccactgctc
    13561 aatcatcacg atagaatccg gtgccataga ctggaaactc acgccagcag cgcaatccac
    13621 cattggcgca tagccgtggc aaccttcttc tttcagtacg gttggaacga ttttagcaaa
    13681 ctgatccaat accgcctgac ggtgatgttg accaggacga gtacggattt ctgcgattac
    13741 ggtaagcatg gttaactcct tctaaagcct gagctctagt taaccaaaaa tttccacaag
    13801 atgcttgcga tattcttcag tatagcgggg aacatcaggc atttttatca cgtcattagc
    13861 gataaatgtc ggcagcggtt ccatgccaag gaattggttt gctttatgga acggcagata
    13921 cacaccgtcg acgccaacgc cgtggaagaa ttgatctttt tcggtgaagg cttccattgg
    13981 tgcgttccag gtcagagaaa gcatatattt tttgccctgt accaggccgc cggaaccgta
    14041 tttttttgac ggatctttgc gggtacggcc atcgctggca tacagcgtcc cgtgaccttc
    14101 ggtgaataca tcatcaatgt attttttcac tgtccacggc gcgcccatcc accagcctgg
    14161 catctgccag atcaccacat cagcccagag aaagttttgt acttccgctt tgacatcgta
    14221 gtcgctgtcg gcgcgaacga tgcgaacatc atgcccgagg tcgcgcagtg tgccatccgc
    14281 gacttcggtc agggtgtcgt tcagttgacc attggagtgg gcgaattttt tcgcgccgtt
    14341 gataatcagg gtgttgctca ttttttaacc tcaaggtgag acgtttgtcg actatagtac
    14401 gtcacagagc ggtacggtaa aattagcaaa atgtgcaaag tcttttgcga atttagcaaa
    14461 agtcttaccc ttaccagctc accttcgcct caaatccacc ttgttccgca ttcccaaatt
    14521 caacattcat gccatgcaat ttggcgattc gctggacaat cgatagccca agtccactgc
    14581 cggtagcggt ttgtccgggg gggcgataga agcgttcgcc aattcgcgcc agtgcctctg
    14641 gtgtcacacc ggggccgtta tccctcacga tgaaattatc ggcattcagc gtgacgtcca
    14701 ccacgctgcc ctgtggactg tagcgcactg cgttatccag aaggtttcgc accaacaaac
    14761 ttagcaatag cggttgtcca gtgcgtttga tgctgtgggc attgagtgtc agtcgcacgt
    14821 caattttcgc ctgctgcgcc gtgtggtaaa tatccatcac cgacgattgc aggagatctt
    14881 caagcgggat ctccgcgacg tcctgaaggt tatccagtga gtccagccgc gatagcgtga
    14941 gcagttgatc aaccagacga gtagcgcgat cgatcccgga atgtaattgg agcagtgctt
    15001 ttttccgcgc ctgcggatca tcgtcagaga gctgcgcaac ttcggtttgc actttcagcg
    15061 ctgttaacgg gctacgtagt tcgtgagctg catcggaggt aaagcgtcgt tctcgaacca
    15121 tcatcgcatg tgtgcgggcg aacagttgat ttagcgactc aaccagtggt cgcacttcgc
    15181 tgggtacgcc agtcgcgttt agtggttttt ccgagtcagg gtcacgcata cgtagtgcca
    15241 gcgccagttt gttcagcggc gcgagttcac gacccagtag taccatcatg atgattaaca
    15301 taatcggcag tgcgaccagc cacgggatca attgcccggc aacaatcgcc agcgccatgt
    15361 cttcacggta ttcccattcc tggccaacaa cgatgcgata tttgccatca ggtgaggtca
    15421 tccagacaaa acgccaagga tcgtcttcac cgaccagttg cccgtcagca aaaccttccc
    15481 gttgatagct atagggaata tcttctccgt tatcgccatc attaaggacc attctgccgt
    15541 cgtgggtaaa gatggcaaag gtcagcgcat cgtcatcaac atgaccgtgt tttaatttat
    15601 ttggcgtctg tgccatgcga tccgccgcgt tgatttcgtt gagatcgagc gtacttaacc
    15661 gcttggcaaa cagcatcagt tgggtgtcga acaattcatc gacgttatcc gttgtttgtt
    15721 tccaggcgac aaagctggaa agcagccagg tcaccgaggc cagaattaaa aagattagcg
    15781 tcagcctgac gcgcagacta agacgttggg taaatttcat ttctcaccta atgtgtagcc
    15841 aataccatgc acggtacgaa taaaatcact accgagtttg cgtcgcagat gatgcacatg
    15901 cacttcaacg gcattactgg tgacctcttc gtcccaggta tacagtttct cttcaatcag
    15961 tttgcgcggc agtacccgac cagcgttacg catcagtaat tccagcaggg caaattcttt
    16021 tggtttcagt gttaagggtt cgccagccag cgtggcgata cgtttgccgg ggtcgagcat
    16081 gacgttaccg tggcgcagct cgttgctggc ctggccgttg gttcggcgca tcagagcctc
    16141 cagcctggcg gcgacttcta tcaacgcaaa aggtttacac agataatcgt cagctcccag
    16201 acgcagccct tctacacgtt ccgctaacgc atcacgcgcg gtcaggatca gtaccggctc
    16261 acgctgacct ttctctcgcc attcgcgcaa aatatcgcga ccatccatgc ctggtaaggt
    16321 taaatccagg atcaccgcat cataaggtgc gctataaagc gcctcttttc cctgacgacc
    16381 ttgtgtaaac cagtcgacac taaaacccat tttactaagg ccagttttga tgccgtcgcc
    16441 aatcagcatg tcatcttcta tcagtaaaat tcgcattttt tcatccctgc gataaccgta
    16501 tttgctgcca gtaaaccgtg ttgtaacgcg ctctgtacag cattaaattt tatttttttc
    16561 ccttaagaag ttgttaagga ctatcttgtt aaatgctcgg aaacagacat taaagggagt
    16621 aataaacatg aaaaaattcg cagcagtaat cgcagtaatg gccctgtgca gtgcaccggt
    16681 gatggcagca gagcagggcg gtttttctgg cccatcggca acgcaaagtc aggccggagg
    16741 attccagggg ccgaacggca gcgtaacgac tgtagaaagc gcaaaatccc tgcgtgacga
    16801 cacctgggta accctgcgcg gcaatatcgt tgaacgcatc tctgacgatc tctacgtgtt
    16861 taaagatgcc agcggtacta tcaatgttga tatcgaccac aaacgctgga acggtgtgac
    16921 ggtgacgccg aaagatacgg ttgagattca gggtgaagtc gataaagact ggaattctgt
    16981 tgaaattgac gtcaaacaga tccgcaaagt aaatccgtaa ttgttaccgc tcccgggacg
    17041 cgttcccggg aataatttcg cagggaggca aaatgacaaa cctgacactg gatgtaaaca
    17101 ttatcgattt cccatcaatc cctgtggcga tgttgccgca ccgctgtagc cctgagttgc
    17161 tcaactacag tgtggcgaaa tttatcatgt ggcgtaaaga gacggggctt tctcctgtta
    17221 accaaagcca gacttttggc gtcgcctggg acgaccctgc caccaccgca ccggaagcgt
    17281 ttcgctttga tatctgcggc agcgttagcg aaccgattcc cgataatcgt tatggtgtga
    17341 gcaatggtga acttaccggt ggacgttatg ccgtggcccg ccacgttggc gagctggacg
    17401 atatttcaca cacgatatgg ggcattattc gccactggct gcctgcaagc ggcgagaaaa
    17461 tgcgtaaagc accgattctg tttcactaca ccaatcttgc cgaaggggtg acggagcagc
    17521 gactggaaac ggatgtttat gtgccgttag cgtgatgggg aatctttacc ggagtgtgag
    17581 ccaaatcact cgcgataagt ttgagcgaac aatgccacag gcgtattgtt gttgggctta
    17641 taatttctcc ggtaaggaat accatgtata cgcgaaattt attatccggt gcgccgtaag
    17701 ccgtctcggg ggtacccatg agcaagccgc acttatggca ggaggcgcgg ggcactggtg
    17761 gagacacagc taaactgggc tgaaccatat acttacagtc gactactttg atgctttagg
    17821 cttccctgga ctcgcataac ctcaacttct cgaatcgccc ggagcggacc cccatgccga
    17881 gtggtgtggc atgggcgcaa ccttgtgctg ccctctatgc cgatcaagtc tacagcaaag
    17941 cgttgtatat caaggcacat tgatgccttt atcgccgtcg gagcacgtcc ggcggcgcta
    18001 ttcacgctga tctcctgtga ctcgacgcgg cagataatgt agtatctccg gcaatattgc
    18061 ccctttgaag gctggcgaat cagttgagga atcagaatta atgagcgata tggcagagcg
    18121 ccttgcgcta catgaattta cggaaaacgc ctacttaaac tactccatgt acgtgatcat
    18181 ggaccgtgcg ttgccgttta ttggtgatgg tctgaaacct gttcagcgcc gcattgtgta
    18241 tgcgatgtct gaactgggcc tgaatgccag cgccaaattt aaaaaatcgg cccgtaccgt
    18301 cggtgacgta ctgggtaaat accatccgca cggcgatagc gcctgttatg aagcgatggt
    18361 cctgatggcg cagccgttct cttaccgtta tccgctggtt gatggtcagg ggaactgggg
    18421 cgcgccggac gatccgaaat cgttcgcggc aatgcgttac accgaatccc ggttgtcgaa
    18481 atattccgag ctgctattga gcgagctggg gcaggggacg gctgactggg tgccaaactt
    18541 cgacggcact ttgcaggagc cgaaaatgct gcctgctcgt ctgccaaaca ttttgcttaa
    18601 cggcaccacc ggtattgccg tcggcatggc gaccgatatt ccaccgcata acctgcgtga
    18661 agtagctcag gcggcaatcg cattaatcga ccagccgaaa accacgctcg atcagctgct
    18721 ggatatcgtg caggggccgg attatccgac tgaagcggaa attatcactt cgcgcgccga
    18781 gatccgtaaa atctacgaga acggacgtgg ttcagtacgt atgcgcgcgg tgtggaagaa
    18841 agaagatggc gcggtggtta tcagcgcatt accgcatcag gtttcaggtg cgcgtgtact
    18901 ggagcaaatt gctgcgcaaa tgcgcaacaa aaagctgccg atggttgacg atctgcgcga
    18961 tgaatctgac cacgagaacc cgacccgtct ggtgattgtg ccgcgttcca accgcgtgga
    19021 tatggatcag gtgatgaacc acctcttcgc taccaccgat ctggaaaaga gctatcgcat
    19081 taacctcaat atgatcggtc tggatggtcg tccggcggtg aaaaacctgc tggaaatcct
    19141 ctccgaatgg ctggtgttcc gtcgcgatac cgtgcgccgc cgactgaact atcgtctgga
    19201 gaaagtcctc aagcgcctgc atatcctgga aggtttgctg gtggcgtttc tcaacatcga
    19261 cgaagtgatt gagatcattc gtaacgaaga tgaaccgaaa ccggcgctga tgtcgcggtt
    19321 tggccttacg gaaacccagg cggaagcgat cctcgaactg aaactgcgtc atcttgccaa
    19381 actggaagag atgaagattc gcggtgagca gagtgagctg gaaaaagagc gcgaccagtt
    19441 gcagggcatt ttggcttccg agcgtaaaat gaataacctg ctgaagaaag aactgcaggc
    19501 agacgcgcaa gcctacggtg acgatcgtcg ttcgccgttg caggaacgcg aagaagcgaa
    19561 agcgatgagc gagcacgaca tgctgccgtc tgaacctgtc accattgtgc tgtcgcagat
    19621 gggctgggta cgcagcgcta aaggccatga tatcgacgcg ccgggcctga attataaagc
    19681 gggtgatagc ttcaaagtgg cggtgaaagg taagagcaac caatcggtag tgtttgttga
    19741 ttccaccggt cgtagctatg ccatcgaccc gattacgctg ccgtcggcgc gtggtcaggg
    19801 cgaaccactc accggcaaat taacgttgcc gcctggggcg accgttgacc atatgctgat
    19861 ggaaagcgac gatcagaaac tgctgatggc ttccgatgcg ggttacggtt tcgtctgcac
    19921 ctttaacgat ctggtggcgc gtaaccgtgc aggtaaggct ttgatcacct taccggaaaa
    19981 tgcccatgtt atgccgccgg tggtgattga agatgcttcc gatatgctgc tggcaatcac
    20041 tcaggcaggc cgtatgttga tgttcccggt aagcgatctg ccgcagctgt cgaagggcaa
    20101 aggcaacaag attatcaaca ttccatcggc agaagccgcg cgtggagaag atggtctggc
    20161 gcaattgtac gttctgccgc cgcaaagcac gctgaccatt catgttggga aacgcaaaat
    20221 taaactgcgc ccggaagagt tacagaaagt cactggtgaa cgtggacgcc gcggtacgtt
    20281 gatgcgcggt ttgcagcgta tcgatcgtgt tgagatcgac tctcctcgcc gtgccagcaa
    20341 cggtgatagc gaagagtaat atgccgggga gggcctgttt cctcccgctt gcaaggaacg
    20401 ccggatgaaa cgttaagtcg tcatccggca gcgtattcaa cccccggaaa cgataaaaat
    20461 tcccttgaaa ccgttgttta ttcatgcgtt gcgattaaca atacgctttt ccagagagcg
    20521 gcttttaaca atgccctaac ctgatttcag gtgacgtaca atgccagtct ctcagacctt
    20581 cagagggtgt tatgctatat atctttcgtc ttattattac cgtgatttac agcatcttag
    20641 tctgtgtatt cggctccatt tactgccttt tcagcccgcg taacccgaaa catgtggcca
    20701 cctttgggca tatgtttggc cgtcttgcgc cgctgttcgg cctgaaagtt gagtgccgca
    20761 aacctgcaga tgctgaaagc tacggcaatg ctatctatat cgctaaccac cagaacaact
    20821 atgacatggt gacagcatcg aacattgtgc aaccgccgac ggtgacggta ggtaaaaaga
    20881 gcttgctgtg gatccccttc ttcgggcagt tgtactggtt aaccggcaac ttattgatcg
    20941 acagaaacaa tcgcactaaa gctcacggca ccattgcgga agtcgtgaat catttcaaaa
    21001 aacgccgtat ttccatctgg atgttcccgg aaggaacccg cagccgtggt cgcggcctgc
    21061 taccgttcaa gactggagca tttcacgcgg caattgcggc gggcgtcccg attattcccg
    21121 tgtgcgtctc tacaacttcg aataagatta atcttaatcg actgcacaac ggtctggtga
    21181 ttgtcgaaat gctgccgcca attgacgtca gtcagtatgg caaagatcag gttcgtgagc
    21241 tggctgccca ttgtcgttcg ataatggaac aaaaaatcgc cgagctcgat aaagaagtcg
    21301 cagaacgcga agccgccgga aaagtttaag tcggcaatct gtatttttgc ggggaacact
    21361 ttcctgcacg gtattacttt agccagtttt acatggagca aatatgtcac tcagtcggcg
    21421 tcagttcatt caggcatcgg ggattgcact ttgtgcaggc gctgttcccc tgaaggccag
    21481 cgcagccggg caacagcaac cgctacccgt tccgccgcta cttgaatctc gccgtgggca
    21541 accgctgttt atgactgtac aacgtgcgca ctggtcattt acgccaggga cacgcgcgtc
    21601 ggtctgggga atcaatggtc gttacctggg gccgactatc cgcgtctgga agggcgacga
    21661 tgttaagctt atttacagca accgcctgac agaaaatgtc tcaatgacgg tggccgggct
    21721 acaggtacct ggcccgctga tgggcggtcc ggcacggatg atgtcgccaa acgctgactg
    21781 ggcacccgta ctgcctattc gccagaacgc agctactctg tggtatcacg ccaatactcc
    21841 caaccgcacg gctcagcagg tctataacgg ccttgccgga atgtggctgg tggaagatga
    21901 agtcagcaag tcgctgccta tccccaacca ttatgatgtg gatgattttc cggtcattat
    21961 ccaggataaa cggctggata actttggtac gccagaatac aacgaaccgg gaagcggcgg
    22021 ctttgttggt gatacgctgc tggttaacgg tgtacaaagc ccgtacgttg aagtctcgcg
    22081 tggctgggtg cgcttgcgac tgctgaacgc gtcgaactct cgtcgctatc aactacagat
    22141 gagcgatggt cgcccgttac atgtgatttc tggcgatcag ggattcctgc ctgctcctgt
    22201 atcggtgaag caactttcgc tggcaccggg cgagcgccgc gagattctgg tggatatgag
    22261 caacggcgat gaagtgtcga tcacctgtgg cgaagcggcg agcattgttg atcgtattcg
    22321 cggcttcttt gagccatcca gtattctggt ttctaccctg gtgctaacgc tgcgcccaac
    22381 cggccttctg ccgctggtca cagacagtct tccgatgcgc ttgctgccaa ctgaaatcat
    22441 ggccggttcg ccgattcgca gtcgcgatat cagtctgggt gatgacccgg gtattaatgg
    22501 acagctgtgg gacgtcaacc gtattgatgt caccgcgcag caaggaacgt gggaacgctg
    22561 gacggtacgc gcggacgagc cgcaagcgtt ccatattgaa ggcgtgatgt tccagatccg
    22621 taacgtgaat ggtgcgatgc cgttcccgga agacagaggc tggaaagata ccgtttgggt
    22681 tgacggacaa gtggagctgc ttgtttattt cggtcagcct tcctgggcgc acttcccgtt
    22741 ctacttcaac agtcagacgc tggaaatggc ggaccgtggc tcgattgggc aactgttagt
    22801 caatccggta ccgtaatctt catactcgcc cgaggagggc gagtatggaa tacttttcga
    22861 tttctctttc gttccggctt ataatccccg gcctttgatt atttttttat catttctgga
    22921 agcaaaatga gctctatctc cctgatccaa ccggatcgcg acctgttctc ctggccgcag
    22981 tactgggccg cctgttttgg accggcaccg tttttgccga tgtctcgtga agagatggat
    23041 caacttggct gggatagctg cgacatcatt ttggttactg gcgacgcgta tgtcgatcac
    23101 ccaagcttcg ggatggcgat ttgcggtcgt atgctggaag cacagggctt tcgcgtcggg
    23161 atcatcgccc agccggactg gagcagcaaa gacgacttta tgcgtctggg taaaccgaat
    23221 ctgtttttcg gtgttactgc tggcaacatg gattcgatga tcaaccatta taccgccgat
    23281 cgccgtttac gtcatgacga tgcctacacg ccggataacg tcgcgggtaa gcgcccggat
    23341 cgcgccacac tggtttatac ccagcgttgt aaagaggcgt ggaaagatgt accggtgatc
    23401 ctcggcggta ttgaggctag tctgcgccgt accgcacatt atgattactg gtccgatacc
    23461 gtgcgccgtt ccgtgctggt ggattcgaaa gccgacatgc tgatgtttgg taacggtgag
    23521 cgtccgctgg tggaggtggc gcaccgtctg gcgatgggcg agccgattag tgaaatccgc
    23581 gatgtgcgta ataccgcgat tatcgtgaaa gaggcgctgc ctggctggag cggcgtggat
    23641 tccccccgtc ttgatacccc tggaaaaatc gacccaatcc cgcatccgta tggtgaagat
    23701 ttgccgtgcg cggataacaa accggtggca ccgaaaaagc aggaagccaa agccgtaacc
    23761 gtgcagccac cgcgcccgaa accgtgggaa aaaacctacg tgttgctgcc ttctttcgag
    23821 aaagtgaagg gcgataaagt gctgtacgcc catgcttcgc gtattctgca ccacgaaacc
    23881 aacccaggct gcgtccgcgc attgatgcaa aaacacggtg accgctatgt gtggatcaac
    23941 ccgcctgcta tcccgctttc taccgaagag atggacagcg tttttgcgct gccgtacaag
    24001 cgcgtgccac atccggccta tggcaatgcc cgtattccgg cttacgaaat gatccgtttt
    24061 tcggtcaaca ttatgcgcgg ctgctttggc ggctgttctt tctgttctat taccgagcac
    24121 gaagggcgca ttattcagag ccgttcggaa gattcgatca ttaatgagat cgaagcgatc
    24181 cgcgacaccg ttccaggttt tacgggcgtg atttccgatc tcggtgggcc aactgccaac
    24241 atgtatatgt tgcactgcaa atcgccacgc gctgaacaaa cttgtcgccg tttgtcgtgc
    24301 gtttatccgg atatttgtcc gcacatggac actaaccacg aaccgacgat caacctctat
    24361 cgccgtgcgc gtgatctgaa aggcattaaa aaaatcctca tcgcctcagg cgtacgttat
    24421 gacatcgccg tggaagatcc gcgctatatc aaagagttgg cgacccatca cgtcggcggt
    24481 tatctgaaga ttgctccgga acataccgaa gaagggccgt tgtcgaagat gatgaagccg
    24541 ggcatgggca gctatgaccg ctttaaagag ctgttcgata cctactcgaa acaggcaggt
    24601 aaagagcagt atctgatccc gtatttcatc tctgcgcacc ccggtacgcg tgatgaagat
    24661 atggtgaatt tggcgctgtg gctgaaaaag catcgtttcc gtctcgacca ggtacagaac
    24721 ttctacccat cgccgctggc gaactcaacc accatgtatt acaccgggaa aaaaccgctg
    24781 gcgaagattg gttataagag cgaagacgtc ttcgtaccga agggcgacaa acagcgtcgt
    24841 ttgcataaag cgttgttgcg ttaccacgat ccggcaaact ggccgttaat ccgcccggcg
    24901 ctggaagcga tgggcaaaaa gcatctgatt ggcagccgtc gcgattgctt agtgcctgcg
    24961 ccaaccattg aagagatgcg tgaagctcgt cgccagaacc gcaatacccg tccggcgttg
    25021 actaaacata cgccgatggc gacccagcgt cagacgcctg ctacggcaaa aaaagcgtcg
    25081 tctacgcaat ctcgtccggt gaatgctggt gcgaagaaac gccctaaagc ggcggttgga
    25141 cgttaaaaaa aatccccggc aactgacatg ctgccgggga tttttttatc attctgcgta
    25201 catacgcaag cagcgcaggc aatcaaaata gtcctgagca tcaagacgcg tattggctct
    25261 gttagcttcg gatttggcag catagatttg tgggcgtagt gcttttatat cctgctcaag
    25321 ccgggcgatt ttggcattta atgtttgtac attgctcgcc agttgattga ccttttgctc
    25381 attgacgcag ccactgagca agcaggttgc cagaacaaca gctcccacgg tgaaaatcgt
    25441 tttcatatag tgttcccttt tatttattgt tccggctaat tgttatttaa ttcagctcat
    25501 tgctttaatc tggcggcggt ttttgcctca atctgcttaa gtaggctgcc gctgttcggg
    25561 cggataaaat cagcgatttc ccacttgcct tcgcgccgct gtaaaaccag ttcttgctct
    25621 tcgttgttgc cattggtcag cgtaaaacga acatgaatgc gggcggaatc ggcggttttc
    25681 tccaggactc gaatttcatc aacgcgtgaa aatagcgact cttcgccaaa acagatcgga
    25741 ttgtcgtaaa taaattgccg ttctatttgc atatcttcat cgggattatt ggccgtcttg
    25801 aaggtggttt tatagaaggc attaaccgcc tggcggtaag agtcggaaac cacccacgtc
    25861 cacggccagc tataatcagc aatatgttca aagagttcgg caacaaaggc ttccggctgt
    25921 tctttttgca acgaagctaa agcggccagc gttttttcat tctcgctatt aactgcttgt
    25981 aagacgctgc catgattgct gacaatatcg tcaatgaccc aacgaccatt ttccgccacc
    26041 attttcagtg tctgcgtggt cttttcttta tcatctttaa agatacgaaa gcgcacaacg
    26101 gcatcggcat gatcggcgtc agtttgggtt atcgcaacgc tttctagcac cagatcgcca
    26161 aaatcctgac aatcacaaac cggatcataa tccagccagc caatattgcc cggcagcgta
    26221 agattgtcgt tcagggtaag cgcctgttga atacgcgctg aagttatcgc ccgctctccg
    26281 gtttcaccga aataaggtgt actggcatct gatttatagt tctgataaat ctgccg
//
LOCUS       DAJVXP010000048        25773 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVXP010000048 DAJVXP010000000
VERSION     DAJVXP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.048"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OQS10_001910"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OQS10_001910"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS2292946.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="OQS10_001911"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="OQS10_001911"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS2292947.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="OQS10_001912"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="OQS10_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS2292948.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="OQS10_001913"
     CDS             2440..3063
                     /locus_tag="OQS10_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS2292949.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OQS10_001914"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OQS10_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS2292950.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="OQS10_001915"
     CDS             complement(3447..3512)
                     /locus_tag="OQS10_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292951.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OQS10_001916"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OQS10_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS2292952.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="OQS10_001917"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="OQS10_001917"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS2292953.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OQS10_001918"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OQS10_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS2292954.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OQS10_001919"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OQS10_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS2292955.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OQS10_001920"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OQS10_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS2292956.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OQS10_001921"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OQS10_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS2292957.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OQS10_001922"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OQS10_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS2292958.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OQS10_001923"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OQS10_001923"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS2292959.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="OQS10_001924"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="OQS10_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS2292960.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="OQS10_001925"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="OQS10_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS2292961.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="OQS10_001926"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="OQS10_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS2292962.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="OQS10_001927"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="OQS10_001927"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS2292963.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="OQS10_001928"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="OQS10_001928"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS2292964.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="OQS10_001929"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="OQS10_001929"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS2292965.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="OQS10_001930"
     CDS             23127..23711
                     /locus_tag="OQS10_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2292966.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="OQS10_001931"
     CDS             23816..24556
                     /locus_tag="OQS10_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS2292967.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25773
                     /gene="htrE"
                     /locus_tag="OQS10_001932"
                     /pseudo
     CDS             24591..25773
                     /gene="htrE"
                     /locus_tag="OQS10_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6984 c         6416 g         6043 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aac
//
LOCUS       DAJVXP010000049        25648 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVXP010000049 DAJVXP010000000
VERSION     DAJVXP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.049"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OQS10_001933"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OQS10_001933"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS2292968.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OQS10_001934"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OQS10_001934"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS2292969.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OQS10_001935"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OQS10_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS2292970.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OQS10_001936"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OQS10_001936"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS2292971.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="OQS10_001937"
     CDS             complement(5499..5696)
                     /locus_tag="OQS10_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS2292972.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OQS10_001938"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OQS10_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS2292973.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OQS10_001939"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OQS10_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS2292974.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OQS10_001940"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OQS10_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS2292975.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OQS10_001941"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OQS10_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS2292976.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OQS10_001942"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OQS10_001942"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS2292977.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OQS10_001943"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OQS10_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS2292978.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OQS10_001944"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OQS10_001944"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS2292979.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OQS10_001945"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OQS10_001945"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS2292980.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OQS10_001946"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OQS10_001946"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS2292981.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="OQS10_001947"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="OQS10_001947"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS2292982.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="OQS10_001948"
     CDS             complement(17138..17191)
                     /locus_tag="OQS10_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2292983.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="OQS10_001949"
     tRNA            complement(17425..17500)
                     /locus_tag="OQS10_001949"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="OQS10_001950"
     tRNA            complement(17536..17611)
                     /locus_tag="OQS10_001950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="OQS10_001951"
     tRNA            complement(17769..17844)
                     /locus_tag="OQS10_001951"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="OQS10_001952"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="OQS10_001952"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS2292984.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="OQS10_001953"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="OQS10_001953"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS2292985.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="OQS10_001954"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="OQS10_001954"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS2292986.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="OQS10_001955"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="OQS10_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS2292987.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25648)
                     /gene="yjeM"
                     /locus_tag="OQS10_001956"
     CDS             complement(24308..>25648)
                     /gene="yjeM"
                     /locus_tag="OQS10_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCS2292988.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5935 a         7194 c         6499 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcattaa
//
LOCUS       DAJVXP010000050        25542 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVXP010000050 DAJVXP010000000
VERSION     DAJVXP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.050"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            362..916
                     /locus_tag="OQS10_001957"
     CDS             362..916
                     /locus_tag="OQS10_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS2292989.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(892..1149)
                     /locus_tag="OQS10_001958"
     CDS             complement(892..1149)
                     /locus_tag="OQS10_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS2292990.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(1146..1964)
                     /gene="aroE"
                     /locus_tag="OQS10_001959"
     CDS             complement(1146..1964)
                     /gene="aroE"
                     /locus_tag="OQS10_001959"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS2292991.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            complement(1969..2541)
                     /gene="tsaC"
                     /locus_tag="OQS10_001960"
     CDS             complement(1969..2541)
                     /gene="tsaC"
                     /locus_tag="OQS10_001960"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS2292992.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            complement(2546..3088)
                     /locus_tag="OQS10_001961"
     CDS             complement(2546..3088)
                     /locus_tag="OQS10_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS2292993.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            complement(3117..3590)
                     /locus_tag="OQS10_001962"
     CDS             complement(3117..3590)
                     /locus_tag="OQS10_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS2292994.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            complement(3562..4686)
                     /gene="dprA"
                     /locus_tag="OQS10_001963"
     CDS             complement(3562..4686)
                     /gene="dprA"
                     /locus_tag="OQS10_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS2292995.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            4816..5325
                     /gene="def"
                     /locus_tag="OQS10_001964"
     CDS             4816..5325
                     /gene="def"
                     /locus_tag="OQS10_001964"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS2292996.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            5340..6287
                     /gene="fmt"
                     /locus_tag="OQS10_001965"
     CDS             5340..6287
                     /gene="fmt"
                     /locus_tag="OQS10_001965"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS2292997.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            6333..7622
                     /gene="rsmB"
                     /locus_tag="OQS10_001966"
     CDS             6333..7622
                     /gene="rsmB"
                     /locus_tag="OQS10_001966"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS2292998.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            7644..9020
                     /gene="trkA"
                     /locus_tag="OQS10_001967"
     CDS             7644..9020
                     /gene="trkA"
                     /locus_tag="OQS10_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS2292999.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            9150..9560
                     /gene="mscL"
                     /locus_tag="OQS10_001968"
     CDS             9150..9560
                     /gene="mscL"
                     /locus_tag="OQS10_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS2293000.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(9557..9775)
                     /gene="arfA"
                     /locus_tag="OQS10_001969"
     CDS             complement(9557..9775)
                     /gene="arfA"
                     /locus_tag="OQS10_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS2293001.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(9831..10256)
                     /gene="zntR"
                     /locus_tag="OQS10_001970"
     CDS             complement(9831..10256)
                     /gene="zntR"
                     /locus_tag="OQS10_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS2293002.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(10267..10635)
                     /locus_tag="OQS10_001971"
     CDS             complement(10267..10635)
                     /locus_tag="OQS10_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS2293003.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            complement(10743..11126)
                     /gene="rplQ"
                     /locus_tag="OQS10_001972"
     CDS             complement(10743..11126)
                     /gene="rplQ"
                     /locus_tag="OQS10_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS2293004.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(11167..12156)
                     /gene="rpoA"
                     /locus_tag="OQS10_001973"
     CDS             complement(11167..12156)
                     /gene="rpoA"
                     /locus_tag="OQS10_001973"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS2293005.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(12182..12802)
                     /gene="rpsD"
                     /locus_tag="OQS10_001974"
     CDS             complement(12182..12802)
                     /gene="rpsD"
                     /locus_tag="OQS10_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS2293006.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(12836..13225)
                     /gene="rpsK"
                     /locus_tag="OQS10_001975"
     CDS             complement(12836..13225)
                     /gene="rpsK"
                     /locus_tag="OQS10_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS2293007.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(13242..13598)
                     /gene="rpsM"
                     /locus_tag="OQS10_001976"
     CDS             complement(13242..13598)
                     /gene="rpsM"
                     /locus_tag="OQS10_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS2293008.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(13745..13861)
                     /gene="rpmJ"
                     /locus_tag="OQS10_001977"
     CDS             complement(13745..13861)
                     /gene="rpmJ"
                     /locus_tag="OQS10_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS2293009.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(13893..15224)
                     /gene="secY"
                     /locus_tag="OQS10_001978"
     CDS             complement(13893..15224)
                     /gene="secY"
                     /locus_tag="OQS10_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS2293010.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(15232..15666)
                     /gene="rplO"
                     /locus_tag="OQS10_001979"
     CDS             complement(15232..15666)
                     /gene="rplO"
                     /locus_tag="OQS10_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS2293011.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(15670..15849)
                     /gene="rpmD"
                     /locus_tag="OQS10_001980"
     CDS             complement(15670..15849)
                     /gene="rpmD"
                     /locus_tag="OQS10_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS2293012.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(15853..16356)
                     /gene="rpsE"
                     /locus_tag="OQS10_001981"
     CDS             complement(15853..16356)
                     /gene="rpsE"
                     /locus_tag="OQS10_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS2293013.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(16371..16724)
                     /gene="rplR"
                     /locus_tag="OQS10_001982"
     CDS             complement(16371..16724)
                     /gene="rplR"
                     /locus_tag="OQS10_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS2293014.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(16734..17267)
                     /gene="rplF"
                     /locus_tag="OQS10_001983"
     CDS             complement(16734..17267)
                     /gene="rplF"
                     /locus_tag="OQS10_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS2293015.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(17280..17672)
                     /gene="rpsH"
                     /locus_tag="OQS10_001984"
     CDS             complement(17280..17672)
                     /gene="rpsH"
                     /locus_tag="OQS10_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS2293016.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(17706..18011)
                     /gene="rpsN"
                     /locus_tag="OQS10_001985"
     CDS             complement(17706..18011)
                     /gene="rpsN"
                     /locus_tag="OQS10_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS2293017.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(18026..18565)
                     /gene="rplE"
                     /locus_tag="OQS10_001986"
     CDS             complement(18026..18565)
                     /gene="rplE"
                     /locus_tag="OQS10_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS2293018.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(18580..18894)
                     /gene="rplX"
                     /locus_tag="OQS10_001987"
     CDS             complement(18580..18894)
                     /gene="rplX"
                     /locus_tag="OQS10_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS2293019.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(18905..19276)
                     /gene="rplN"
                     /locus_tag="OQS10_001988"
     CDS             complement(18905..19276)
                     /gene="rplN"
                     /locus_tag="OQS10_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS2293020.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(19441..19695)
                     /gene="rpsQ"
                     /locus_tag="OQS10_001989"
     CDS             complement(19441..19695)
                     /gene="rpsQ"
                     /locus_tag="OQS10_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS2293021.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(19695..19886)
                     /gene="rpmC"
                     /locus_tag="OQS10_001990"
     CDS             complement(19695..19886)
                     /gene="rpmC"
                     /locus_tag="OQS10_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS2293022.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(19886..20296)
                     /gene="rplP"
                     /locus_tag="OQS10_001991"
     CDS             complement(19886..20296)
                     /gene="rplP"
                     /locus_tag="OQS10_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS2293023.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(20309..21010)
                     /gene="rpsC"
                     /locus_tag="OQS10_001992"
     CDS             complement(20309..21010)
                     /gene="rpsC"
                     /locus_tag="OQS10_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS2293024.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(21028..21360)
                     /gene="rplV"
                     /locus_tag="OQS10_001993"
     CDS             complement(21028..21360)
                     /gene="rplV"
                     /locus_tag="OQS10_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS2293025.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21375..21653)
                     /gene="rpsS"
                     /locus_tag="OQS10_001994"
     CDS             complement(21375..21653)
                     /gene="rpsS"
                     /locus_tag="OQS10_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS2293026.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(21670..22491)
                     /gene="rplB"
                     /locus_tag="OQS10_001995"
     CDS             complement(21670..22491)
                     /gene="rplB"
                     /locus_tag="OQS10_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS2293027.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(22509..22811)
                     /gene="rplW"
                     /locus_tag="OQS10_001996"
     CDS             complement(22509..22811)
                     /gene="rplW"
                     /locus_tag="OQS10_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS2293028.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(22808..23413)
                     /gene="rplD"
                     /locus_tag="OQS10_001997"
     CDS             complement(22808..23413)
                     /gene="rplD"
                     /locus_tag="OQS10_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS2293029.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(23424..24053)
                     /gene="rplC"
                     /locus_tag="OQS10_001998"
     CDS             complement(23424..24053)
                     /gene="rplC"
                     /locus_tag="OQS10_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS2293030.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(24086..24397)
                     /gene="rpsJ"
                     /locus_tag="OQS10_001999"
     CDS             complement(24086..24397)
                     /gene="rpsJ"
                     /locus_tag="OQS10_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS2293031.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(24635..25054)
                     /gene="gspB"
                     /locus_tag="OQS10_002000"
     CDS             complement(24635..25054)
                     /gene="gspB"
                     /locus_tag="OQS10_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCS2293032.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            complement(25056..25526)
                     /locus_tag="OQS10_002001"
     CDS             complement(25056..25526)
                     /locus_tag="OQS10_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCS2293033.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
BASE COUNT         6316 a         6688 c         6035 g         6503 t
ORIGIN      
        1 cacacagatt gtctgataaa ttgttaaaga gcagttgcga cgcggctttc agctcactgt
       61 cgcgaggtgg cgtatattac gctttcctct ttcagagtca accctgaatt tcagaatttt
      121 ttctcttcgt tgttccgacc atcctgtgaa gtgtttcacg ttgtcgtctc aacggaggcg
      181 cattataggg atcccaattt tttgcacaag tacttttttg atcttttttt ctgtttgttg
      241 ttttttcacc ctttttgctg cattcgcaca caaaacggtg cttttttgca tactaaaaga
      301 cttgcacaag gctaataatg cacccaaagt cattaggaaa tcatttattg ttgaggtgag
      361 tatgtctgat gttttacgcc cataccgcga tctttttcca caaatcggcc agcgcgtaat
      421 gatcgacgat agcagcgtcg tgattggtga cgttcgtctg gctgatgatg tggggatctg
      481 gccgctcgtt gtgattcgtg gagatgtaca ttatgtacag atcggagcac gcaccaatat
      541 ccaggatggc agtatgttgc atgtcactca taaatcctcg tacaacccag atggcaaccc
      601 attaaccatt ggcgaagatg tcactgttgg tcacaaggtg atgctccacg gctgcaccat
      661 tggcaatcga gttttggttg ggatgggctc aattttactt gatggcgcaa tagtagaaga
      721 tgatgtgatg attggtgcgg gtagcctggt cccacaaaat aaacggctgg agagcggata
      781 tctgtatctc ggtagtcccg tcaaacagat ccgcccgtta agtgatgaag agaaggctgg
      841 gttacgctat tccgcaaata actacgtgaa atggaaggac gaatatctgg atcaaggtaa
      901 ccagacccaa ccttgatcat cttcactttg ttcctgaatt aagttttccg cttcttcttc
      961 caggtcccag cgatgctgac gaaaactcgc taaccactgt tctggcgtac ctccagtaaa
     1021 gcgatacgcc agactctcgc cagagatcgc acatgtcagt tgcataccat tcacgagagc
     1081 aggaaaacat acacatcttt tattctcgtc ccactcttcc ctatccggaa actggatggc
     1141 ctgattcacg ctgacaattc ctgttgcaat agtttgatga ccggttctac atcaggcaga
     1201 acgccatgcc aaagaaggaa ggcatgagcc gcctgtgcca ccagcattcc taaaccatca
     1261 gcattacgct ttgagcctcg ctgctcacac catgccagaa aaggagtttt tcctttctga
     1321 tagaacatgt catagcaata aatgcctgga tgaatgagcg atgacgggat cgccggaata
     1381 tcaccactga tgccactgga tgttgcatta ataatgagat caaactcatg accttccagt
     1441 tcgtccatac ccaacgcctg aatactgcca gtgtgcgcaa acaatttagc caactcttcc
     1501 gcgcgggata ccgtccgatt agtgattgtc accgcacagt ctagggaaag aagtggcagt
     1561 agtacgccgc gagatgctcc accagcgccg ataagcagaa tacgtaaacc agggcggata
     1621 aaagacagac gttccagatc gcttaacaag cctacaccat cggtattgtc acccagcagg
     1681 cgtccatctt ctaaccgctt gagggtatta actgcaccag ccaacgctgc ccgttcagta
     1741 agctcatccg ctctggcaaa agcctcttct ttaaaaggca ccgtcacatt cgcaccttta
     1801 ccaccagcac gaaagaaagc gttcagtgtg ttgatgaaat cattgatggg tgccaacacg
     1861 cgcccatagg gatgttcaat attcagttgc tgagcaaatt gctgatgaat gaatggcgat
     1921 ttgctgtggg ctatcggatt accaaaaaca gcataggttt ccattatgtt acccctgtcg
     1981 aaacagttca cccgtcaggg catcgcggat ttctgaagga tttaaacgcc cccccgtttc
     2041 accaggcaca accgggaacg ccgcgccaaa ttgtgcgcga acttcgtcta ctgttcgaca
     2101 aggtggcaat ccactcaagt tggcactggt agaaaccagc ggtttaccat aagcctggca
     2161 caaagcaacc accaacggat ggtcggtgac tcgtacagca agcgaatcaa agcggcccgt
     2221 caaccagcgc ggtgttgtcg caggcgcggg aaagacaaag gtgacaggac ctggccagcg
     2281 ggaaaaaatg gtttcacgct gcgcatcagt cagcatggtg tcatcaatat agggtttaag
     2341 ctgctcgtaa tttgccgcga ttaaaatcag ccccttatca accggacgct gttttaactc
     2401 caacagtctc atcactgctg tttcgctatc aggatcgcac ccaacaccga aaacggcttc
     2461 cgttggatag gcgatgacac gttcttcatt gaggacatct atcgcagctg cgatagcgtc
     2521 tccttgcagg ttattattca cgttattatt ccgccgaaac cggctttcca cattgtttac
     2581 tggcacaaaa gtgttttaca ccctgcgcgg ttttcttttc gatgagtagc ggataatgac
     2641 actcagggca ttctccagct attggtttga agttaatggc aaattgacac tccgggtagc
     2701 gatcacagga gtgaaatgtt ttgccataac gggagcggcg ctggaccaga tggcccgtcc
     2761 gacattgggg gcatgtaatt gctgtttcgt ccggtttatc gataagttcg gtatgttcgc
     2821 attcagggta gttactgcaa ccaataaaca taccaaagcg tccctggcgt aataccagat
     2881 ttgcgccaca tgcagggcaa acctgcccct ccagaacttt gacaatatgt ccatccgctg
     2941 aagatttcag aagacggacg tagtcacacg ccggatactg tgagcatcca agaaacggac
     3001 cgtgtttccc ggatcgaata accagttcag ccccgcactt tgggcaggac tcattattac
     3061 gcaccgtgaa cagtgctgat tttgccataa caacttatgc tgaattaaag aaatgattaa
     3121 tgcagcatac cttcattcac ttcaaagagt aattcttcca tttgctggta cgcattttcg
     3181 caccccggga tgttgaacaa caccatcagg atcacccatt tcagatcatc cagttcgaac
     3241 tctgcgttat ccagcgcgag cactcgctct atcaccattt cacgagtttc aaggttgagc
     3301 acctgaatct gctcaaggaa aagcagaaac ccacggcagc tggcatccag tcgttcacac
     3361 tcttccggtg tataaatacg catggagaga ggatccgagg ccagttgcat cggttctgcc
     3421 aacccttcct gataatcagc aagtttttcc agccatagca gggcattgta gatatcttct
     3481 cgctcaaaac ctgcgtcggt aagatcctgt tcaagtttgt cttgatccac acgcaactca
     3541 gcttcagtgt gaatataggt ttcaaacaaa tacattagta cgtcgaacat ggcatgccct
     3601 cctcaatcgg acatagccgc cgggtacagc tgcgatccat cctgctaact ccagttcgag
     3661 tagttgagta actacctctg gcacaggttg gccggcacgt tcagcgacga cgtcaacagg
     3721 tgttacctca tctcctacgt tagccaggag ctcaggaaat ggcaatgcca cgtcttgctg
     3781 atctggtgaa taaaatgaat tttcaggggc gtctggcaac cagtgcaatc caaattgcaa
     3841 gttttccaga atttcttccg gttccgtcac aagaatcgca ccttgtttta ttaaccagtg
     3901 aggcccttcg cttcccggat tccctattgg acctggcaag gcaaaaactt cacgcctctg
     3961 ctcaagcgca caacgtgctg tcaccagcga accactgcgc aaagccgctt ccaccaccag
     4021 tacaccttta cttagaccac tgataatgcg atttcttcgt gggaaattgt aagcaagggg
     4081 tggaacatcg aggggaaatt ccgagacgag agctccgcca tgttcaagca gactggcagc
     4141 cagtcgggca tggcggcggg gatgaatggt attaagtcca ttccccaata cagcaatgct
     4201 gacgccattt acctgtaagg cagctttatg cgccacacca tcgattccac gcgccagtcc
     4261 actcgtaatt gtcactccac gcgtcgccag agtttcgcaa aataaccgtc cccatcgctc
     4321 gccataccat gaatgcgccc gactccccac tacggcaagc tgaaatgaat gcagcgcgtg
     4381 cagttctcct tcaacaaaca gtgcgccggg gtaatctgtc gtcaccagaa gttgaggagg
     4441 ataaaattcg ctgtccgcag ggattaaatg atggttgggt tgctccaacc aacaaagtga
     4501 gctttcgata ctctttcgcg gaaatgaaag aaagcgttgt gcctgccgca atgtaagccc
     4561 tgtttgctgc aatccaaccg catcaatttg cgactgtttt gccagccagt gagctatacg
     4621 gaccatatca tcgccgtaca agctgctgat actcattaaa cgcagccaaa tttctgtatc
     4681 gaccatcctt atctccctgc cataagcagc cttagcaatc tttgcgattg gtcagtgatg
     4741 ctgtcaatca gagggggatt tgtctagaat agaagaaata atctttctaa cccctgaaca
     4801 catctctgga gatttatgtc agttttgcaa gtgttacata ttccggacga gcggcttcgc
     4861 aaagttgcta aaccggtaga agaagtgaat gcagaaatac agcgtatcgt cgatgatatg
     4921 ttcgagacga tgtacgcaga agaaggtatt ggcctggcgg caacccaggt tgatatccat
     4981 caacgtatca ttgttattga tgtttcggaa aaccgtgacg aacggctggt gttaatcaat
     5041 ccggagcttt tagaaaaaag cggcgaaaca ggcattgaag aaggttgcct gtcgatccct
     5101 gaacaacgtg ctttagtgcc gcgcgcagag aaagttaaaa ttcgcgccct tgaccgcgac
     5161 ggtaaaccat ttgaactgga agcagagggg ctgttagcca tctgtattca gcatgagatg
     5221 gatcacctgg tcggcaaact gtttatggat tatctgtcac cgctgaaaca acaacgtatt
     5281 cgtcagaaag ttgaaaaact agatcgtctg aaagcccggg cttaaggata agaactaacg
     5341 tgtcagaatc actacgtatt atttttgcgg gtacacctga ctttgcagcg cgtcatctcg
     5401 acgcgctgtt gtcttctggt cataacgtcg ttggcgtgtt cacccagcca gaccgaccgg
     5461 caggacgcgg taaaaaactg atgcccagcc cggttaaagt tctggctgag gaaaaaggtc
     5521 tgcccgtttt tcaacctgtt tccctgcgtc cacaagaaag ccagcaactg gtcgccgatc
     5581 tgcaggctga tgttatggtc gtcgtcgcct atggcttaat tctgcctaaa gcagtgctgg
     5641 agatgccgcg tcttggctgt atcaacgttc atggttcact gctgccacgc tggcgcggtg
     5701 ctgcaccaat ccaacgctca ctatgggcgg gtgatgcaga aactggtgtg accattatgc
     5761 aaatggatgt cggtttagac accggtgata tgctctataa gctctcctgc ccgattactg
     5821 cagaagatac cagtggtacg ctgtacgaca agctggcaga gcttggccca caagggctaa
     5881 tcaccacgtt gaaacaacta gcagacggca cggcgaaacc agaagttcag gacgaaactc
     5941 ttgtcactta cgccgagaag ttgagtaaag aagaagcgcg tattgactgg tcgctttcgg
     6001 cagcgcagct tgaacgctgc attcgcgctt tcaacccatg gccaatgagc tggctggaaa
     6061 ttgaaggaca accggttaaa gtctggaaag cgtcggtcat tgatacggca accaacgctg
     6121 caccaggaac gatccttgaa gccaacaaac aaggcattca ggttgcgact ggtgatggca
     6181 tcctgaacct gctctcgtta caacctgcgg gtaagaaagc gatgagcgcg caagacctcc
     6241 tgaattctcg tcgggaatgg tttgttccgg gcaaccgtct ggcctgatag tccactcttc
     6301 taagcccggt cttaccgggc atttttatac ttatgaaaaa acaacgtaat ttacgtagca
     6361 tggcggccca ggccgttgaa caagtcgtcg agcaagggca atcattaagc aacattctgc
     6421 caccgctcca gcaaaaagtt tctgataaag acaaagcact tcttcaagag ttgtgctttg
     6481 gcgtactgcg tacgctttcg cagttagact ggctgattaa taagttaatg gcccgtccga
     6541 tgaccggcaa acagcgcact gtgcattacc tgattatggt tggtttgtat caactgcttt
     6601 atacccgcat tccacctcat gctgcgctgg ctgaaacggt tgaaggcgct atcgcaatta
     6661 agcgtccgca acttaaaggg ttgattaacg gtgtattacg ccagttccag cgtcagcaag
     6721 aagagttatt agccgagttt aataccagtg atgcacgtta tctgcatcct tcctggttgc
     6781 tgaagcgtct gcaaaaagcg tatccagagc agtggcaatc catcgtcgaa gccaataacc
     6841 agcgtccgcc aatgtggctg cgcgttaatc gtacgcatca ttcccgcgac agctggcttg
     6901 cattgttgga tgaagcagga atgaaaggtt tcccgcatgc ggattaccct gatgctgtac
     6961 ggctggaaac acctgcacct gttcatgcgc tacctggttt tgaagacgga tgggttaccg
     7021 ttcaggatgc atcagcacaa ggttgcatga cctggcttgc gccacaaaac ggtgaacaca
     7081 ttctggatct ttgtgccgcc cccggcggta aaacaacgca tatccttgag gtggcaccag
     7141 aagcgcaggt tgttgcggtt gatattgacg aacagcgcct ctctcgcgtt cacgacaatt
     7201 taaaacgcct tggtatgaag gcgaccgtga aacaaggtga tggccgttac ccttcccaat
     7261 ggtgtggcga gcaacagttt gatcgcattt tattagatgc gccttgttca gcaacggggg
     7321 tgattcgtcg ccatccggat attaaatggt tacgtcgcga tcgcgatatc ccggaactcg
     7381 cgcaattgca gtctgaaatt ctcgacgcca tttggccgca tttaaaatcc ggtggaaccc
     7441 tggtctatgc cacctgttcg gtgttaccgg aagagaatag cctgcagatt aaagcttttt
     7501 tgcaacgtac cgctgatgcc gaactttgcg aaacaggaac accagagcaa ccgggtaaac
     7561 aaaatctgcc tggtgccgaa gagggcgacg gcttctttta cgctaagcta atcaaaaagt
     7621 gatgagataa cgggtcgaga ctgatgaaaa ttatcattct gggtgccggc caggttggcg
     7681 gcacactggc ggaaaacctg gttggcgaga acaacgatat tactgttgtc gataccaacg
     7741 gtgagcgtct gcggaccttg caggataaat ttgacctgcg agtcgtgcag gggcatggct
     7801 ctcatccacg cgtattgcgg gaggcaggtg ccgacgacgc cgatatgctg gttgctgtaa
     7861 ccagttcaga tgaaaccaat atggttgcct gccaggtagc ctactcactt ttcaacaccc
     7921 ctaatcgcat cgctcgcatc cgctcaccag actacgtacg cgatgccgat aagctatttc
     7981 attcagatgc tgtaccgatt gatcatctga tcgcaccaga gcagttggtt atcgataata
     8041 tttaccgact gattgagtat ccaggcgcat tgcaggtggt gaacttcgct gagggtaaag
     8101 tcagcctcgc tgtagttaaa gcctattatg gcggcccgct gattggtaat gcactttcga
     8161 ccatgcgcga acatatgcca catatcgata ctcgtgtggc agcaattttc cgccacgatc
     8221 gccccattcg tccacaaggt tcgaccattg ttgaagctgg tgatgaagtg ttctttattg
     8281 ccgcttcaca gcatatccgc gcggtgatga gtgaattaca gcgactggaa aaaccgtata
     8341 agcggatcat gctggttggt ggcggtaata tcggtgcagg gctggcgcgt cgtctggaaa
     8401 aagattacag cgttaaactc atcgaacgta atcagcagcg cgctgccgaa ctggcggaaa
     8461 agttgcagaa tacgatcgtc ttttttggtg atgcgtcgga tcaagaactg ctggccgaag
     8521 aacatatcga tcaagttgat ctgtttattg ccgtcaccaa cgatgacgag gccaatatca
     8581 tgtccgccat gcttgccaaa cgtatgggtg cgaaaaaggt gatggtattg atccagcgtc
     8641 gcgcttatgt ggatctggtt caggggagcg tgatcgatat tgcaatttca ccacaacaag
     8701 caactatttc tgcgttgctt agccatgtgc gaaaagcaga tattgttggt gtttcctcat
     8761 tgcgccgcgg cgtagcagaa gctattgaag ccgttgctca cggtgatgaa agcacctcac
     8821 gcgttgtcgg cagagtcatt gacgaaatca agctaccgcc aggaacgatt attggagcgg
     8881 tggtacgtgg aaacgacgtg atgattgcca atgacaatct gcgcattgag caaggcgatc
     8941 acgtaattat gttcctcaca gataaaaagt ttattaccga cgtcgaaaga ctcttccagc
     9001 caagcccttt cttcttgtaa ttaataaggc gtctaatgac gccttattat ttccctttga
     9061 ttatcaagga ttaattaaat tcattcctgg caggaaaatg gcttaacatt tgttagactt
     9121 atggttgtcg gcttcatagg gagaataaca tgagcattat taaagaattt cgcgaatttg
     9181 cgatgcgcgg gaacgtggtg gatttggcgg tgggtgtcat tatcggtgcg gcattcggga
     9241 agattgtatc ttcactggtt gccgatatca tcatgccacc tctgggctta ttaattggcg
     9301 ggatcgactt taaacagttt gctgtcacgc tacgcgatgc gcagggggat atccctgctg
     9361 ttgtgatgca ttacggtgtc ttcattcaaa acgtctttga ttttctgatt gtggcctttg
     9421 ccatctttat ggcgattaag ctaatcaaca aactgaatcg gaaaaaagaa gaaccagctg
     9481 ccgcacctgc accaactaaa gaagaagtat tactggcaga aattcgtgat ttgctgaaag
     9541 agcagaataa ccgctcttaa caagcgcctg aaagcagaag accagtggta aaaaagtgat
     9601 ttactttctt gccactggcc tcccagttcc cccgattgcc atgttttcct tttcgcatgt
     9661 aactgccttt ccctttctta tttttctcta ctcgctgtcg gaataaggga tcatgtagta
     9721 atgcttctat cgcattatcc tttatctgcc ctttagtatg ctgatatcgg ctcataaaaa
     9781 ctccagttgg ttatttaacg gcgcgagtgt aatcctgtca gtgcaaaaaa tcaacagcca
     9841 ctcttaacgc cactcgcccc ttgttcaaga gcttcaagaa tcgagcaata aacactgcta
     9901 tgggcggtcc cacaacaggc atcgttaagg cgttgcaagg aacgctgcat actctgcaac
     9961 tcggctatcc gtgcttcgac ttcctgcaat ctttcctgca caatgccttt tgactcctga
    10021 caggtatggt gttcaggatc gatgcggatc gacagcaact cgcggatcga ctccagactg
    10081 aaacctagtt gtctggcatg gcggataaat ttcaatcgct ggagatcgct ttcggtatac
    10141 aggcgaaacc cgccttcagt acgcacttca tgctccatca tctgctgttt ttcgtaataa
    10201 cgaatcgtgt caggtgttac ttccgccatt tttgccagct caccaatgcg atacatacat
    10261 actccactag ttatcgttga ttttgtccaa caacttgtca gcataatcgc cgcgtaaaaa
    10321 atcggtactc aatccagctt gtcgcagctt caattccagt aatgacaatc gacggctaac
    10381 ctcttgatat tgcggatcat cgtgacggat acctttgaga atatccagaa gctgaattgc
    10441 ttctctccgt tgctcaagtt ctggcggtaa gcaaccggca ttcttcagca agcgataccc
    10501 cgcacgtaat tccggtggca cgtgagaatc atcatccagt atcaatggtt cgccgctacc
    10561 tgccaggtta tcaaactcac ctttcgcttg cgcttctgct atatggcgct ctgcccactg
    10621 gtcaagtaac cacataaata ctccagggga tgaacaaaaa gagtacagct attgtagata
    10681 agtggggata ctacgggtat aaaaaaaacc cgccggggcg ggttttttta cattgcttca
    10741 gattactctg cagcagcttc tgctttctct gaacgatcaa ccagctcgat gtaagccatc
    10801 ggcgcgttgt cgcctgcacg gaagccacac ttcagaatac gagtgtaacc accggcacgg
    10861 ctcgcgaaac gcgggcccag ttcgttaaac agttttgcca cgatctcgtt atcacgagta
    10921 cgggcaaatg ccagacgacg attagcaacg ctatcagtct tggcaagagt aatcagcggc
    10981 tcaactacgc ggcgcagctc tttcgcttta ggcagagtcg tcttgatgat ttcatgacga
    11041 accagtgaac ctgccatatt gcggaacata gcctggcgat ggctgctgtt gcggttcagt
    11101 tgacgaccac tcttacgatg gcgcatgacc ttatccttct cagtaaaacc ttaacctgtg
    11161 atccggttac tcgtcagcga tgcttgccgg tggccagttt tccaggcgca tgcccagaga
    11221 cagtccacgg gaagccagca cgtctttaat ctcagtaaga gattttttac caaggttagg
    11281 cgttttaagg agctcaacct cggtacgctg taccagatca ccgatatagt ggatagcttc
    11341 tgctttaagg cagttagcag agcggacagt caattccaga tcgtcaacag ggcgcagcag
    11401 gatcggatcg aactctggtt tctcttcttt cacttcaggc tgacgtacat cacgtaagtc
    11461 aacgaaagct tccagttgtt cagccagaat ggttgccgca cgacgaatcg cctcttcagg
    11521 atcgattgtg ccgttggttt ccatttcgat gaccagcttg tccaggtcgg tacgctgttc
    11581 tacacgcgct gcttcaacat tgtaggcaat acgctccaca gggctgtagc atgcgtcgac
    11641 cagcagacgg ccgattgggc gctcatcttc ttccgaatga attcgggtag aagccggcac
    11701 ataaccacga ccgcgctgaa ctttgatacg catgctaata gacgcgttct catcggtcag
    11761 gtggcagatc acgtgctgcg gcttgacgat ttcgacatca ccgtcgtggg tgatatcggc
    11821 tgcagtcaca gggccaatgc cagatttatt caaggtaaga ataacttcat ctttgccctg
    11881 aactctcacc gccagccctt tcaggttgag caggatttcc aggatatctt cctgaacgcc
    11941 ttctttggtg ctgtactcat gtagtacacc atcaatctca acctcggtca ccgcgcaacc
    12001 cggcatcgat gagagcagaa tacggcgcag tgcgttaccc agagtatggc caaagccacg
    12061 ctctaaaggc tcaagggtca ccttggcgtg cgtcgaactc acttgctcga tatcaaccag
    12121 gcgcggtttt agaaactctg tcacagaacc ctgcattgtg tcctctcttt ggtactaagc
    12181 tttacttgga gtaaagctcg acgatcaggt gttcgttaat gtccgcagac agatcagaac
    12241 gctccggctt acgcttaaac gtaccttcca tcttgccagc atcaacttcc agccaggttg
    12301 gcttttcacg ctgctcagcc agctccagag cggctttcac gcgagactgc ttcttcgctt
    12361 tctcacgaat gcttacaacg tcattcggac taacctgata agaagcgatg ttaacaacac
    12421 gaccgtttac cataattgct ttatggctaa ccagctgacg tgcttctgca cgagtggcac
    12481 cgaagcccat acggtataca acgttgtcca gacgaccttc cagcagagcc aacaggtttt
    12541 caccggtgtt gcctttcaga cgtgctgctt ctttgtagta gttacggaac tgacgctcca
    12601 gcacaccata gatacggcga actttttgct tttcacgcaa ctgcacacca tagtcagaca
    12661 gacgcggttt acgcgcaccg tgctggccag gagcttgttc aattttacac ttggtatcga
    12721 tcgcgcgaac gccagactta aggaataagt cggtgccctc acgacggctc agcttgagct
    12781 taggacccaa atatcttgcc attttctttc tccaacaaac ctggaaaacg aggcgttata
    12841 cgcgacgttt tttcggcgga cgacaaccgt tatgagggat cggagtcaca tcagtaatgt
    12901 tagtgatgcg gaaacctgcg gcgttcagag cacgaatagt agattcgcgg cctggacccg
    12961 gacctttaac cataacttcc agattcttga tgccgtattc tttcacggcg tcagcgcaac
    13021 gctctgctgc aacctgagct gcaaacggag tggatttgcg agaaccacgg aaaccggaac
    13081 caccggctgt tgcccaaccc aacgcgttac cctgacgatc agtgatagtc acgatggtgt
    13141 tgttgaaaga agcatggata tgagccacgc cgtcagagac ttgttttctt acacgtttac
    13201 gtgcacgaat tggtgccttt gccattattc aatcaccccg attatttctt gatcggtttg
    13261 cgcggaccct tacgggtacg tgcgttggtc ttggtacgct gaccgcgaac cgggagacca
    13321 cgacgatgac gcaaaccgcg atagcaacca agatccatca ggcgcttgat gctcatgctg
    13381 atttcacggc gcagatcacc ttcaacgaca aatttggcaa cttcgtcacg cagcgtgtcg
    13441 atttgtcctt cagacagctc actgatctta acatcttcag cgatacccgc tgcagccagg
    13501 atggctttag aacgggtctt gccgacgcca taaatcgaag ttaatgcgat tacggcatgc
    13561 ttatgatcag gaatgttaat gcctgctata cgggccacta tgcactccta ctatttaata
    13621 tgtacgttcc atgctgaaaa gcccgttttc aggatactca aatggaaacg cacagacata
    13681 caaaagattg gctgactaat ctagccagct caacccaact ttgcaagaaa aatatgcgaa
    13741 aaaatcagcc ttggcgctgt ttatgcttcg gctcggcact gcaaatcaca cggatgacac
    13801 catcacgctt aacgattttg cagttacggc ataatttctt gacggaagca cgaactttca
    13861 tttttactct ccgtaacttc tcgggcgacc aattatcggc cgtagccttt caggttcgcc
    13921 ttcttcaatg cagactcata ctgactggac atcatcagag tttgcacttg agccataaag
    13981 tccataatca cgacaacaac gataagcagt gaggtcccac cgaagtagaa cggtactttc
    14041 attgcatcac gcatgaactc cgggatcagg cagataaagg taatatacag cgcaccaacc
    14101 agggtcaggc gggtcattac tttatcgata tacttcgccg tttgctctcc cggacgaatt
    14161 cctggtacaa atgcaccgga cttcttcagg ttatctgctg tttcacgcgg gttgaaaacc
    14221 aacgccgtgt agaagaaaca gaagaagatg attgcagacg catagagtaa cacataaagc
    14281 ggttgcccag gctgcaaata cagcgaaatt gttgtcagcc agttccaacc agtaccgccc
    14341 ccgaaccatg acgcgatggt cgccgggaac agaataatac tggaagcgaa gattgccggg
    14401 attacccccg ccatattcac tttcagcggt aaatgtgtgc tctgtgcagc ataaacacga
    14461 cgaccttgct gacgtttcgc gtagtttacc acaatgcggc gttgaccacg ctcaacaaat
    14521 acaacaaaga acgtcactgc aaatactaat actgcaacca acagcaacac gaggaagtgc
    14581 aggtcgcctt gacgcgcttg ctcgatagta tgggcaatgg ctggcgggag tcccgcgaca
    14641 ataccgacga agataatgat tgaaataccg ttgccgatac ctcgttcagt aatctgttcg
    14701 cccaaccaca tcaggaacat cgttcctgtg accagactta caacagcggt gaagtagaat
    14761 gcaaagcccg ggttaatcac caggccttgc ataccaggca tattcggcag accagtagca
    14821 ataccgatcg actggaatat tgccagcacc agagtaccgt agcgggtgta ctggctgatc
    14881 ttacgacgac cagactcccc ttctttctta atttccgcca acgttgggtg aaccaccgtc
    14941 agcagctgga taatgatcga cgccgaaata tacggcatga tccccagagc aaagatagaa
    15001 gcacggctga gagcaccacc agagaacatg ttaaacatct caatgatggt gcctcgctgt
    15061 tgctcaagca gtttggcaag tacagcggca tcaataccag ggatcggaat aaaagagcca
    15121 atacggaaca caatcagcgc accgataaca aacagcagtc tgcgtttcag ctcgcctaag
    15181 ccacctttgg cactttgaaa atctaatccc ggttgtttag ccatctgcta cttattcctc
    15241 gattttaccg ccagcagctt cgatagcagc acgagcgcct ttagtaacac gcaggccacg
    15301 aacagttacc ggagtcgtta cttcgccagc caggatcact ttcgcgaact cgatctggat
    15361 accgataatg ttagccgctt tcagcgtgtt caggtctaca acaccgcctt ctactttagc
    15421 caggtcagac agacgaactt cggctgtaat cgctgcttta cgagaagtga agccgaattt
    15481 cggcagacga cggtacagag gcatctgacc accctcgaaa ccgcgacgta cgccaccgcc
    15541 agaacgagac ttctgacctt tgtgaccacg accaccggtt ttaccgaggc cagaaccgat
    15601 accacgaccc aggcgtttac ccgccttttt ggagccttcg gccggagaca gagtatttaa
    15661 acgcatctct tactcctcaa ctttaaccat gaaggaaacc gcgttgatca taccgcgaat
    15721 agcaggagta tcctcgcgct ctacggtgtg accaatacga cgcagaccca ggccaagcag
    15781 cgttgccttg tgtttcggca gacgaccgat tgcactgcgg gtttgagtaa ttttaatagt
    15841 ctttgccatg gtttatttcc ccagaatttc ttcaacggat ttaccacgct tggcagcgac
    15901 catttctgga gaattcatat tttccaggcc atcaatagtt gcacgaacca cgttgatcgg
    15961 gttggtggaa ccatatgctt tagccagaac gttatgaacc ccagcgactt ccagaacggc
    16021 gcgcattgca ccaccggcga tgataccggt accttcggaa gccggctgca tgaatacgcg
    16081 agaacccgtg tgaacacctt taacagggtg ttgcagagtg ccgttattca gcgcgacgtt
    16141 aatcatattg cgacgggctt tttccatcgc tttctggatc gctgctggaa cttcacgcgc
    16201 tttaccgtaa ccaaaaccaa cgcgaccgtt accatcgcca actacagtca gagctgtgaa
    16261 ggagaaaata cgaccacctt taacggtttt agatacgcgg tttaccgcga tcagcttttc
    16321 ctgcagttcg ccagcttgtt tttcgatgtg agccatctta cacctctacc ttagaactga
    16381 aggccagctt cacgggcagc atctgccagt gcctggacac gaccatgata ttggaacccg
    16441 gaacggtcaa aggatacatc tttgatgcct ttttccagag cgcgttcagc gacagcttta
    16501 cccacagctg cagccgcgtc tttgttaccg gtgtacttca gttgttcagc gatagctttt
    16561 tctacagtag aagcagctac cagaacttca gaaccgttcg gtgcaattac ctgtgcgtaa
    16621 atgtgacgcg gggtacgatg taccaccagg cgagttgcgc ccagctcctg gagcttgcgg
    16681 cgtgcgcggg tcgcacgacg gatacgagca aatttcttat ccatagtgtt accttacttc
    16741 ttcttagcct ctttggtacg cacgacttcg tcggcgtaac gaacaccctt gcctttataa
    16801 ggctcaggac gacggtaggc gcgcagatcc gctgcaacct ggccgatcac ctgcttatca
    16861 acgcctttca gcacgatttc agtctgagtc ggacattcag cagtgatacc cgcaggcagc
    16921 tgatggtcaa caggatgaga gaaacccaga gacaggttaa tcacattgcc tttaaccgct
    16981 gcacggtaac ctacaccaac cagctgcagc ttcttagtga agccttcggt aacaccgata
    17041 accattgagt tcagcagggc acgcgcggta ccagcctgtg cccaaccgtc tgcgtaacca
    17101 tcacgcggac cgaaggtcag ggtattatct gcatgtttaa cttcaacagc atcgttgaga
    17161 gtacgagtca gctcgccgtt tttacctttg atcgtaataa cctgaccgtt gatttttacg
    17221 tcaacgccgg caggaacaac gaccggtgct ttagcaacac gagacatttt ttcctccgat
    17281 taggctacgt agcagataat ttcgccacca agaccagcct ggcgcgctgc acgatcagtc
    17341 ataacacctt tagaggtaga aacaactgcg atacccagac ccgccataac tttcggcagc
    17401 tcatctttac gtttatagat gcgcagacct gggcggctga cacgctgaat gctttctaca
    17461 acagctttgc cctggaaata cttcagagta agttccagtt caggcttggt gtcgccttca
    17521 actttaaaat cttcaataaa accttcttcc ttcagcacgt tggcgattgc cactttcagc
    17581 ttggaggaag gcatggtgac cgcagctttg ttcgcggcct gaccgttacg gatacgggtc
    17641 agcatatccg cgatcggatc ttgcatgctc atctgtcttt actcccgtga ttcaattggt
    17701 gacaattacc agctagcctt tttcagaccc gggatttcac cgcgcatagc ggcttcacgg
    17761 accttaatac ggctcaaccc gaacttccgc aggaaaccat gcgggcgacc tgtttgacgg
    17821 cagcggttac gctgacgaga cgggctggaa tcacgcggca gagtctgcag cttgagaaca
    17881 gcgttccaac gatcttcgtc ggaagcgttc acatcagaga tgatcgcttt cagttcagcg
    17941 cgtttcgcga agtatttatc agctaaagct acgcgtttta cttcgcgtgc tttcattgat
    18001 tgcttagcca tttagtaacc ctaccttact tgcggaacgg gaagtcaaag gcagccagca
    18061 gagcgcggcc ttcttcgtca gatttcgcag tagtggtaat ggtaatatcc aaaccacgaa
    18121 cgcggtcgac tttatcgtag tcgatttctg ggaagatgat ctgctcacgg acacccatgc
    18181 tgtagttacc acgaccgtcg aaagacttag cggacaggcc acggaagtca cgaatacgag
    18241 gtacagcaat agtgatcagg cgctcaaaga actcccacat gcgttcgcca cgcagagtta
    18301 ctttacagcc gatcggatag ccctgacgga ttttgaagcc tgcaacagat ttgcgtgctt
    18361 tggtgatcag cggtttttga ccggagattg ctgccaggtc tgctgctgcg ttatccagca
    18421 gttttttgtc agcgatcgct tcaccaacac ccatgttcag ggtgatcttc tcgacccgag
    18481 ggacttgcat gacagaattg tagttaaact cagtcatgag ttttttaact acttcgtctt
    18541 tgtagtaatc atgcagtttc gccatcgtac tactccaaat tacttgatag tttcgctgtt
    18601 agacttgaag aaacggactt ttttgccgtc ttcgaatcta aagcctacac ggtcagcctt
    18661 gccggttgcc gcattgaaga ttgctacgtt ggaaacctga atagcggctt ctttttcaac
    18721 gatgccaccc ggttggttca gggccggaac cggcttctga tgtttcttaa ccaggttgat
    18781 accttcaaca atgaccttgc cggaagacag gacattctta actttaccgc gtttaccttt
    18841 atctttaccg gttaacacga taacttcgtc atcacgacgg attttcgctg ccatgattcg
    18901 ctccttagag tacttctggt gccagagaga taattttcat gaacttctca ctacgaagct
    18961 cacgagttac cggcccaaaa atacgcgtac cgataggctg ctcgctgttg ttgttcagaa
    19021 gaacacaagc attaccatcg aagcgaatga cagaaccgtc cgggcgacga acacccttct
    19081 tggtgcgcac cactaccgcc ttcagcacat cacctttttt gaccttacca cgcggaattg
    19141 cttctttgat ggtgatcttg atgatgtcgc ctacgcctgc gtagcgacgg tgcgagccac
    19201 ccagaacctt gatacacatt acgcgacgtg caccggagtt gtcggcgacg ttcagcatag
    19261 tctgttcttg gatcatttta gtgctccgct aatgtcaact actactgaga cccgaaaatc
    19321 aggtcgttaa aaatccccat atcgagggcg cggcattata acaccgcttc aaggatatgg
    19381 gtagaaaaaa taaacggctc atttctgagc cgtttattcg tatcgagaga gtgtactgta
    19441 ttacagaacc gctttctcta caacgcgaac cagcgtccag gatttagtct tggacagcgg
    19501 acggcattcg cggatttcaa ccacgtcacc gataccgcat tcgttgttct cgtcatgtac
    19561 gtgcagtttg gtcgtacgct tgatgaattt accgtagatc gggtgtttca caaaacgttc
    19621 gatagcaaca acaatggatt tctccatttt gtcgctaaca acgcgacctt gcagagtacg
    19681 gattttatcg gtcattacgc acccgccttc tcgttcagta aagtcttaac gcgtgcgaca
    19741 tcgcgacgca cttgcttcaa caggtgagac tgttgcagct ggccacttgc agcctgcata
    19801 cgcaggttga actgctcacg cagcaggttc agcagctcgg tgttcagctc ttcaacgctc
    19861 ttctcacgca gctcttttgc tttcattaca tcaccgtctt agttacaaag gtggttttaa
    19921 tcggcagttt cgctgctgcc agcttgaatg cttcacgggc cagctcttcc ggaacaccgt
    19981 ccatttcata caggacttta cccggctgaa tcaaggcaac ccaatactcc acgttacctt
    20041 tacctttacc catacgcact gccagcggct tttcagtgat cggtttgtcc gggaacacac
    20101 ggatccagat cttaccttga cgcttaactg cacgggtcat agcacgacgt gctgcttcga
    20161 tctgacgggc agtcagacga ccacggccaa cagctttcag accgaagctg ccgaagctaa
    20221 catccgtacc ctgcgccaga ccgcggttac ggcctttgtg cattttacgg aattttgtac
    20281 gctttggttg taacatcagc gacgctcctt atttacggcc tttacgctgc tgctttttag
    20341 gctgagcagc cggtttttcc ggttgttcaa cagcagccat accacccagg atctcgcctt
    20401 tgaagatcca cactttaacg ccgattacac cgtaagtggt gtgcgcttca gaggtgttgt
    20461 agtcgatgtc agcacgcaga gtgtgcagcg gtacgcgacc ttcgcggtac cattcggtac
    20521 gtgcgatttc cgcgccgccc agacggccgc taacttcaac tttaatacct ttagcgccca
    20581 gacgcattgc gttctgtaca gcacgcttca tagcacgacg gaacataacg cgacgttcca
    20641 gctgagaagt gatgctgtca gcaaccagtt ttgcgtccag ttcaggctta cgaacttcgg
    20701 cgatgttgat ctgtgcagga acgccagcga tgtccgctac gaccttacgc agtttttcta
    20761 cgtcttcacc ttttttaccg ataacgatac ccgggcgagc agtgtgaatg gttacacgga
    20821 tgctcttagc cggacgctcg ataacgatac gagatacaga cgctttagcc agttccttag
    20881 tcaggtactg acgtacttta aaatcgctgt ccaggttgtc agcgaattct ttggtgttcg
    20941 caaaccaggt agagttccat ggttttacaa tacccaagcg aataccatta ggatgtactt
    21001 tctgacccat tgctagtctc cagagtctca gcgatcggac acaaccacag tgatgtggct
    21061 ggtgcgcttc aggatgcgat ctgcacgacc ttttgcacgc ggcataatgc gcttcatgct
    21121 cgggccttcg tctacgaaaa ttttcgtaac tttcagatcg tcaatgtcag cgccatcgtt
    21181 gtgttcagcg ttagcaatgg cagattccag aactttcttg accagtacag ccgctttctt
    21241 gttggtgtag gtcaaaatat ccagagcctg cgacactttc ttaccgcgaa tcaggtcagc
    21301 aacaaggcga accttctgag cagaagaacg agcatggcga tgtttagcga tagtttccat
    21361 ctcttcctcc taccttattt cttcttcgct tttttatcag cagcgtggcc gcgataagta
    21421 cgagtcggtg cgaattcacc cagtttgtga ccgaccattt cgtcggtcac aaataccgga
    21481 acgtgctgac gaccattatg gacagcgatg gtcaaaccga tcatgttagg aaagatcgtt
    21541 gaacgacggg accaagtgcg caggggcttc ttgtctccgc tttccaccgc tttctctacc
    21601 ttcttcagca agtgcaggtc aataaaagga cctttcttga gagaacgtgg catggcttat
    21661 cctctaaaat tatttgctac ggcgacgtac gatgaattta tcagtacgct tgttgctgcg
    21721 ggtcttctta cctttggtct gaacgcccca cggagttacc gggtgcttac caaagttacg
    21781 accttcacca ccaccatgtg ggtggtctac cgggttcatc gcggtaccgc gaacggtcgg
    21841 acgaacacca cgccagcgtg cagcacctgc tttacccaga acgcgcagca tatgctcagc
    21901 attgccaact tcgcccagag ttgcacggca gtctgcttct actttacgca tttcaccaga
    21961 acgcagacgc agggtgacat aagcaccatc acgagcaacg atctgaacgt aagtaccagc
    22021 ggaacgtgcc agctgaccgc ctttacctgg tttcatttct acgttatgaa cagtagaacc
    22081 aaccgggatg ttgcgcatcg gcagggtgtt acctggtttg attgcagcat caacgccaga
    22141 ctgaatctgg tcgccagctt tcaggccttt aggggccagg atgtaacggc gttcaccgtc
    22201 tttgtacaga accagcgcga tgttcgcgga acggttcgga tcgtactcaa gacgttcaac
    22261 aactgccggg ataccgtctt tgttgcgttt gaagtcaaca atacggtaag cctgcttgtg
    22321 gccaccaccg atatgacgag tggtgatacg gccattgttg ttacgaccac cggatttgct
    22381 gtttttttcc agcaacggag caaaaggttt gcccttgtgc agctcagggt taaccacttt
    22441 aactacgtgg cgacgacccg gagatgtcgg tttacattta acaactgcca ttgtattact
    22501 cctccgactt actcagcgcc gccaacgaag tccagattct ggccttcttt cagggtgacg
    22561 taagcttttt tccagtcgct acgacgaccg atacgctgtc cgtgacgttt aactttccct
    22621 ttaactacca gggtgttaac gacttcgact tcgacttcaa acagtttctg cacagcagct
    22681 ttgatttctg ctttggtcgc gtctttagca actttgagta cgatggtgtt ggatttttcc
    22741 atcgcagtag acgctttttc agaaacgtgc ggtgcacgca gcaccttcag cagacgttct
    22801 tcacgaatca tgccagcatc tcctcaactt gcttaacagc atcagcagtc attacgactt
    22861 tgtcgaaggc gatcaggcta accgggtcga taccagttgc atcgcgtacg tcaaccttgt
    22921 gcaggttgcg cgcagccagg aacaggtttt cgtccagctc accggtgatg atcagcacat
    22981 cttccagagc catgtctttc agtttctgtg ccagcagctt agttttcggc gcttctacag
    23041 agaacttctc gacaacgatc agacgatcct gacgtaccag ttcggacagg atgcttttca
    23101 gcgcgccgcg gtacatcttc ttgttaactt tttgactgtg gtcctgcgga cgagcagcaa
    23161 aggtcacgcc accagaacgc cagatcgggc tcttgataga accagaacgc gcacggccgg
    23221 tgcctttctg gcgccacggt tttttaccgg aaccagttac ttcagcacga gtcttctgag
    23281 cacgagtacc ctgacgagca ccagctgcat aagcaacaac aacctggtga accagcgctt
    23341 cgttgaaatc acgaccgaag gtagtttcgg aaacagtcag cgcgctctgc gcgtctttca
    23401 atactaattc cattgctatc tccttacgcc ttcacagctg gtttaacgat caggtcgcta
    23461 ccggttgcac ccgggacagc acctttaacc agcagcaggt tgcgctcagc gtcaacgcgt
    23521 actacgtcaa ggctctgaac ggttacacgt tcgttaccca tctgacctgc cattttcttg
    23581 cctttgaaca ctttgcccgg agtctggttc tgaccgatag aacccggaac gcggtgagac
    23641 aaggagttac cgtgagtagc gtcctgggta cggaagttcc agcgcttaac ggtacctgcg
    23701 aaacctttac ctttagaggt gccagttacg tcaacttttt taacctcagc aaacagttca
    23761 acgctaatgc tctgacctac agtgaactct tcgccttcag ccaggcggaa ttcccacaga
    23821 ccacggccag cttctacgcc agctttagcg aagtggccag cttcaggctt ggtcacacgg
    23881 ttagcttttt tagcaccggt ggtcacctga atagcacggt agccatcgtt agccaggtct
    23941 ttaacctgag taacgcggtt tgcttcaact tcgattacgg ttactgggat agaaacgccg
    24001 tcttctgtga agatacgggt catacccact tttttaccga ctaaaccaat cattgtttca
    24061 acctctcaat cgctcaatga cctgattaac ccaggctgat ctgcacgtct acaccggcag
    24121 ccagatccag acgcatcaga gcatcaacgg ttttctcggt tggctcaacg atgtcaacca
    24181 gacgcaagtg agtacggatt tcgtactgat cgcgcgcgtc tttgttgacg tgcggggaga
    24241 tcagaacagt gaagcgctct ttgcgtgtcg gcagcgggat cggaccacgg acctgcgcac
    24301 cagtgcgctt ggcagtctcg acgatttccg cggttgcttg atcgatcaga cgatgatcaa
    24361 acgctttcag gcggatacgg attctttggt tctgcatgag accagagctc caattatttt
    24421 ataaacgaaa atgattactc ctcagaccca ttacgattga tgggagagtg taaccgttct
    24481 tacgtagctc cccgattggg agcattgtta ggtagccaaa ttcggctaac tgaggttcag
    24541 attgaacctg ctgtcaacta cgacaagccc gcgcattata catacttaac caccgaacgc
    24601 aagcgtattg ctagtaaagt aaacagcctt cgggttatct ctcaagttcg ttcagcgcat
    24661 ttttgacacg ctctcttaaa ccggctttct cgtcgctttc ttttacagta ttttcttcgt
    24721 cttcagaaga agagggagca ttctcaaccg cgacgtctgg gtcatcttcc tcctcgacgg
    24781 attcgacgac ggggagtggc tgttttttga agaaatagtg ttgtgttgat tgtttaaaag
    24841 cgggcgtttc agcctctaca gtgacttctg cttttactat ccaaagcgcc catagctgcc
    24901 tggcataccc accaaccaac accattcctg caaaccacag gcaaatcagc aataaacaaa
    24961 tgacatatat tatggtgctg tgtttttgtc gggagaagat accagggtga cctgtttctt
    25021 tctgctcacg cgcggcgata tagaattcaa acatgtcatt tccccttcac tgttgtttcc
    25081 ggggaaatat ttgcttcatc ccgatataag taggcactct cccctgccac ctgccagaga
    25141 tgtactaacg ttgaaaagcc aacaacaccg tcagttttca agtggtggct tttttgaaat
    25201 tgctttattt tttctaccaa caagggacgc cactccgccg aaggttctgt cgaaatatgc
    25261 aatgcacggt ttaacatcgt ttccagccag agaatctctt cctcgctgct atcacgcgta
    25321 atggtactct ccccttccgg cgacattttc cacaacagaa gataatcacc cgtccatacc
    25381 gactcaaacc atttatgtgt cagcgtccac gtttgctgac ctacgaggac atcaacgcta
    25441 gcctcaccga cgcgaacgac cacaacaggc aattttttgt ccccgacttt aagcgatgcg
    25501 ctccagggca aattctgatc taccaatgtt tgtaaagaag ca
//
LOCUS       DAJVXP010000051        25432 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVXP010000051 DAJVXP010000000
VERSION     DAJVXP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.051"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            124..447
                     /gene="soxS"
                     /locus_tag="OQS10_002002"
     CDS             124..447
                     /gene="soxS"
                     /locus_tag="OQS10_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS2293034.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="OQS10_002003"
     CDS             complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="OQS10_002003"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS2293035.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2466..2746
                     /locus_tag="OQS10_002004"
                     /pseudo
     CDS             2466..2746
                     /locus_tag="OQS10_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="OQS10_002005"
     CDS             complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="OQS10_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS2293036.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3635..6457
                     /gene="uvrA"
                     /locus_tag="OQS10_002006"
     CDS             3635..6457
                     /gene="uvrA"
                     /locus_tag="OQS10_002006"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS2293037.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6492..6848)
                     /locus_tag="OQS10_002007"
     CDS             complement(6492..6848)
                     /locus_tag="OQS10_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS2293038.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6852..7268)
                     /locus_tag="OQS10_002008"
     CDS             complement(6852..7268)
                     /locus_tag="OQS10_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS2293039.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="OQS10_002009"
     CDS             complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="OQS10_002009"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS2293040.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8494..8716
                     /locus_tag="OQS10_002010"
                     /pseudo
     CDS             8494..8716
                     /locus_tag="OQS10_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="OQS10_002011"
     CDS             complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="OQS10_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS2293041.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10666..11745)
                     /gene="alr"
                     /locus_tag="OQS10_002012"
     CDS             complement(10666..11745)
                     /gene="alr"
                     /locus_tag="OQS10_002012"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS2293042.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="OQS10_002013"
     CDS             complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="OQS10_002013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS2293043.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13296..14279
                     /locus_tag="OQS10_002014"
     CDS             13296..14279
                     /locus_tag="OQS10_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS2293044.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="OQS10_002015"
     CDS             complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="OQS10_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS2293045.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="OQS10_002016"
     CDS             complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="OQS10_002016"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS2293046.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16221..17206)
                     /locus_tag="OQS10_002017"
                     /pseudo
     CDS             complement(16221..17206)
                     /locus_tag="OQS10_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17524..18039
                     /gene="zur"
                     /locus_tag="OQS10_002018"
     CDS             17524..18039
                     /gene="zur"
                     /locus_tag="OQS10_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS2293047.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18081..18290)
                     /locus_tag="OQS10_002019"
     CDS             complement(18081..18290)
                     /locus_tag="OQS10_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS2293048.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="OQS10_002020"
     CDS             complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="OQS10_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS2293049.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="OQS10_002021"
     CDS             complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="OQS10_002021"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS2293050.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="OQS10_002022"
     CDS             complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="OQS10_002022"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS2293051.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21061..23484
                     /gene="plsB"
                     /locus_tag="OQS10_002023"
     CDS             21061..23484
                     /gene="plsB"
                     /locus_tag="OQS10_002023"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS2293052.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="OQS10_002024"
     CDS             complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="OQS10_002024"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS2293053.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24524..25021)
                     /gene="ubiC"
                     /locus_tag="OQS10_002025"
     CDS             complement(24524..25021)
                     /gene="ubiC"
                     /locus_tag="OQS10_002025"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS2293054.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25244..>25432)
                     /locus_tag="OQS10_002026"
     CDS             complement(25244..>25432)
                     /locus_tag="OQS10_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293055.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
BASE COUNT         6580 a         6543 c         6089 g         6220 t
ORIGIN      
        1 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
       61 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
      121 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
      181 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
      241 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
      301 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
      361 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
      421 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
      481 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
      541 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
      601 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
      661 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
      721 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
      781 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
      841 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
      901 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
      961 gaaacatagc gatcggcatg tgttgccaag tacgcgccca gatcgcggaa gacattatca
     1021 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
     1081 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
     1141 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
     1201 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
     1261 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
     1321 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
     1381 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
     1441 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
     1501 gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact ccagaagaga
     1561 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
     1621 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
     1681 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
     1741 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
     1801 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
     1861 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
     1921 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
     1981 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
     2041 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
     2101 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
     2161 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
     2221 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
     2281 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagc tcacaaaatt
     2341 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     2401 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     2461 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcagctt cttagtgccg
     2521 taatgatgtt tctggccagc acatcaacat ccgttttcgt cggtctgatt atgtcgggct
     2581 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
     2641 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
     2701 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagcctc
     2761 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
     2821 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
     2881 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
     2941 ctgattgcca ccctgcggct gctgaggctg cccccaaccg ccctgcggct gaccaccacc
     3001 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
     3061 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
     3121 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
     3181 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
     3241 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
     3301 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
     3361 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
     3421 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
     3481 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
     3541 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
     3601 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
     3661 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
     3721 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     3781 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     3841 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     3901 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     3961 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     4021 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     4081 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     4141 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     4201 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     4261 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     4321 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     4381 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc
     4441 gtttaacaac ccggcaggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt
     4501 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     4561 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
     4621 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     4681 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     4741 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
     4801 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     4861 cgaaggaacg cgtctgcgtc gggaagcgcg ccacgtgtat gtcgagaata cgccgctgcc
     4921 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
     4981 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     5041 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     5101 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     5161 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     5221 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     5281 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     5341 tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg
     5401 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     5461 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     5521 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     5581 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     5641 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     5701 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     5761 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     5821 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     5881 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     5941 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     6001 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     6061 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     6121 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     6181 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     6241 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     6301 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     6361 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     6421 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     6481 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     6541 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     6601 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     6661 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     6721 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     6781 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
     6841 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     6901 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     6961 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     7021 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     7081 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     7141 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     7201 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     7261 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     7321 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     7381 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     7441 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     7501 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     7561 attttgtatt ttgccctggt ttatcgcccg caaagattac cggattcatg ttggtggcag
     7621 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac
     7681 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     7741 cctcttttgg aatgctgaat tcatcccagc cattattcat tttttcccag aacacaggat
     7801 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aaccccggac
     7861 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     7921 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     7981 caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     8041 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     8101 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     8161 ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     8221 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     8281 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     8341 aatttgaaag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     8401 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     8461 actaatttta ttactcccga tggagatgat gatatgaata tatcatatgt gaatagcaac
     8521 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagacat ctcttatgcc
     8581 aaagattttt cttatgctaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     8641 aaagattttt gtagacctgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     8701 gcatttatcg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     8761 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     8821 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     8881 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     8941 tatattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     9001 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaaggaattc
     9061 cagataattg ggataaactt aacaataaaa gaactgacaa cgttttatta tctctcacgt
     9121 agaacaacgg catcaaaaaa atcatctaca aagaaatagt gaaagcgacg acagtttaat
     9181 ttcactgcag gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc
     9241 ctttgccaca cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc
     9301 gatgagatag acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt
     9361 ataactgaac atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt
     9421 gcttaatacc ttcaccaatt cctgacgcat tgccagaatg cgagtacgca tctcttctac
     9481 ttccgccagc cagctggctt tcaatgcctc gtcattcagc actgcagcca ccacctgcgc
     9541 accaaaattc ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac
     9601 gcggccagcg gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta
     9661 aagggagaaa attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat
     9721 ggcgcgaata gcgtaggcat cctcttccat accggcacta aatccttgat aggcaatatc
     9781 gaggaatggg ataagctcgc gggctttgag aatttcaatc accgcatccc actgatcatt
     9841 agtgagatcg gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc
     9901 aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc acgctgttag tcgcttcgtc
     9961 ataccagggg taagtactca cttcgaatcc agccccggcg aatattgcta cgtggttttc
    10021 ccaggtagga tcgctgaccc agacgcctga ttccgggaag tagcgtttca ggaaatccgc
    10081 gcccactttc aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg
    10141 cagtaccgga tggtccgcac caaacagcag cggcgcaatg gcatggcgat agctgttaag
    10201 cccttccatc ggtaaataaa gcgaagcgcc atggggctgc gcattcaggc gcgcttccgc
    10261 ctccgccacg gctttcagtt gtggaataat tccgtcttcg ttgtagtaca gaccgatact
    10321 taaattcact ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg
    10381 gtcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag
    10441 gtggttaaaa cacaataaac cggaagaagg cgaagatcga gtggatgttc aggagcgaac
    10501 ggcaattagc aacagagtga gactcatgac aaacgaacat ccgccagagg cacgaccttt
    10561 ataagagcat gaaagaacat caacttattg aatttttagg attttattgg ccggataagg
    10621 cattcacgcc gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt
    10681 catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa gcgctcactt ttgtcatttc
    10741 agcgatacgt tctacgggca aaccttcgcc ccataaaatg accggatccc cggctttgtc
    10801 ctgcgcctgt ggacctaagt ctacgcagat catatccatc gccacgcgtc cgacaatcgg
    10861 tacttcgcga ccgttcacca gcactggcgt accggatgga gcggcgcgcg gataaccatc
    10921 gccatagccc atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc aggttccacc
    10981 ataaccaaca ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga
    11041 catcactggc tgacagccaa aatcggcacc gatggagcga tcttccagcg gcgagacgcc
    11101 ataaagaatg atgcccgggc gcacccagtc aaaatgcgat tgtggccaca gcagaatgcc
    11161 acccgacgcg gcaatggaac gttgaccagg tttgccttcg caaaaggtat taaagatagc
    11221 gagttgtttc tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat
    11281 attcaccggc tgacgaacgt ttttgcactg tgtcaggcga tgataaaacg cctcagcctg
    11341 ttccggcctt acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg
    11401 ctcgtccagg ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg
    11461 aaaatgttgc gcagaaatcg tcggcagatc tctggcatca aaaaagcctt cgagtaacag
    11521 tacaggtttg gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc
    11581 aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt
    11641 cgctttcacc accgcaacca ttttactggc aggggccagt tcacgaagac gttgcaggtt
    11701 gtgtcgcaga gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata
    11761 agtgtttgct ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc
    11821 ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg
    11881 ggccgttacg ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt
    11941 gatacacctc atcacgatag ataaacataa tcaagtccgc atcctgctcg atagagccag
    12001 attcacgcag gtcggagttg accgggcgtt tgtcggcacg ttgttccaga gaacggttca
    12061 actgggacag cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcgag
    12121 agatttctgc aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca
    12181 ggtagtcgat catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg
    12241 aacgcacttc cgttggcgtc aggccggagg agtcatcgat atagatattg cgtttttcga
    12301 gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc atcatcgagc tgcccggtac
    12361 ggattttagt ctggtcaacg cgcgacagcg acgccagaga acgcatcata atctgttctg
    12421 aaggcatctc cagcgagaag ataagtactg gtttatcctg caacatcgcc gcgttttcga
    12481 cgaggttcat cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg atgatcaaat
    12541 ccgacggctg caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttactccgg
    12601 taacgccatc gtgtggctgc tgaaacaact gctcaatacg cgccacggtt gcgtcgagca
    12661 catcggcgat gttcttcggc ccttcgtctt tgtttgcacg actttcggca attttaaaga
    12721 cgcgggattc agcaaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg
    12781 cttcagcaat ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga
    12841 tgtcagcata agcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat
    12901 aagcaaaacc accgacgcta tcgagttgcc cctggcgttc cagcgattcc gcaagggtaa
    12961 tcagatcgat aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatggcggt
    13021 gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc tacatcgtcc cagcgttcgt
    13081 tatccagcat taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt
    13141 tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt
    13201 tatttcctgc catagtgaat ggagttaccg agataaagaa tgggtcgaaa ctttaccata
    13261 tgaagcagac cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca
    13321 agcacggtgg cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcagaga
    13381 atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg tatatccgca
    13441 gcggccttta cccgccgcca tcactaccca gcggattagg taccgaagcg gcaggcatcg
    13501 tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta gtctatgcgc
    13561 agtcggcgtt aggcgcttac agctctgtgc ataacattaa tgcggataaa gcggcgattc
    13621 tgcctgcggc aatttctttt gagcaagctg cggcatcctt cctgaaaggc ttaacggttt
    13681 attatctgct gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag
    13741 cggctggcgg cgttggctta attgcctgcc agtgggcaaa agccctgggc gcgaaactta
    13801 tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc gcgtggcagg
    13861 ttattaacta tcgtgaagag gatctggtcg agcggttaaa agagatcacc ggcggtaaga
    13921 aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc
    13981 tgcaacgccg cggcttaatg gtcagttttg gcaactcatc aggtgcggtt accggtgtga
    14041 acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct
    14101 atatcaccac gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca
    14161 gcggtgtgat taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc
    14221 gtgcgcatga gattctggaa agccgggcga cgcaaggttc cagcctgttg attccataaa
    14281 agaaataggg cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta
    14341 cagcacgatg aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt
    14401 gttctaagga taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg
    14461 tcataacgct gatatttcgg cacttttggt gctttaatcg ccttaataac ccacaccacc
    14521 gcaatcgcca gtagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg
    14581 aacataatgg ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg
    14641 accatcagca tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa
    14701 atatgaaatc tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca
    14761 gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt gactactttt tggtgaaaag
    14821 ttaacgctta tccgccacca gtttgagcgc gtgttccagc acattaatgt ctgcacccgc
    14881 tttatgggca ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa
    14941 caagcccaac atatgccggg taatatggcc gagatacgtc ccctggctga gttcacgctc
    15001 aatgtacgga tacatggcgc gcactaccgc caccggatcg gcatcggtat ccgaggaacc
    15061 aaagatctcc cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac
    15121 catcacgcca tccatatgtt gcaggtgtgc tttggcctct tccagcgact tgataccacc
    15181 gttaatcgac attgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc
    15241 gagcggcggg atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc
    15301 gtggatgata aacatctcac actcgccttt gccggaaacg gtgttgatga aatcgcagag
    15361 aaattcatag ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca ctggaatcga
    15421 caccacatcg cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca
    15481 cgcaccaaac atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc
    15541 atcatatcca cgcgcttctg ccagcttcgc acactgtgcc agcgccgccg gatcgctacc
    15601 gccgagttgc aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc
    15661 gtgaataatc gcccctgtgg tcaccatttc ggtatacagc aacgtattgc gggaaagcag
    15721 acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cgctaaaccg
    15781 accactccag tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat
    15841 ttcagaatta ccgtgcattt tcaaatgtag agattatttt atcgatatat catggggtta
    15901 tgttattcag catcactatt caggaggctc aatagcgggg tactatacca taacaacagg
    15961 aagcgcctgt ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga
    16021 agtgatggaa ataatcttat tagccgttaa ctttgttaag gccaatgata aacaatccag
    16081 gttcgacgat aaataaaaaa tcacacatta aactctggtg atatatctcc ctgctaatag
    16141 cattgataga gaaaaaagaa cccaataaat attgggtcct tttatataat gccttccata
    16201 ctatcgaaga acttcacata ttatttctcc gatttaaccc cgaacaaatc ataaattaat
    16261 ttagaagtgt ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa cgacttcgcg
    16321 aagctaatta attgtggtgc agcatctcgc attttgctta aaatatcagg atcgagcgtt
    16381 ccttcattca ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca
    16441 tcagccagac gcttcatctc tcccaggtac ttcttcatat cacgttgaga taatttttca
    16501 aactgagctt tcaggtagct ctcattattt tcattaatat acactgtttc tgataaatct
    16561 gcgtaggcac tagaatatga aagtgaaatt aacagtgaag caaataattt taagctgttg
    16621 tcatcatgtt cctggcaggc attaacaaac gttaaaaaac cagaacctat tgcttgaaaa
    16681 tgtatattcg ctaaaggttc attatcctta gattcctcat aaaatagctg aaccgtggag
    16741 ggaaggccat tcttagtttc tacatcaaaa gtacattgtt caatgggagt cgcatcttta
    16801 ttatccccag gattacatat actattaatc gcgaaatgaa aaatagcccg gtcaacaact
    16861 gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc aatcgtcaat atacttattg
    16921 acccacattt tgagatcatt tatttgctca attaaaaaca cattttttta cgtgcatgcc
    16981 ataaattatg cgtgattcgt tgaataaaca aatctatttt tgcaataatt atatcccgat
    17041 gacgcagcgc tgcataatca atagatttac catatatatt atttaattta cctttgattg
    17101 aacctctatt atctaattta tctttgattg catcttcatt atcgaattga tttgaattga
    17161 gtgttttatt aaagtatcca gttgcaccgg gtattatctt caacatgatt catctacctt
    17221 ttttgtcgta ccgtgtcctc cttataattc ttttgccatc tggtcactat caaaaatcgc
    17281 tcataatttc tgaactacag gtatgcgttt aaacccagtt aaataaatag cgagttaact
    17341 tgaagcaatt tatttaaaaa tgaattatta attattatcg tgttgataat ggtacgacaa
    17401 ggcaacataa caccttacct acaaaacaga aaatcaacat tacggcaaca ataagggttc
    17461 tcggtgtttt tcctggaaca tggtaaagta aggacattct taacccccac tttgaggtgc
    17521 ccgatggaaa agaccacaac gcaggagtta ttagcgcagg ctgaaaaaat ctgcgcgcag
    17581 cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc gcctgatgag tctgcaggat
    17641 ggcgctatca gcgcttatga tctgcttgat ttactgcgcg aagctgaacc gcaagccaag
    17701 ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc aaggttttgt gcataaggtg
    17761 gaatccacca acagttatgt gctctgtcat ctgttcgacc agcccacgca tacgtcagcc
    17821 atgtttatat gcgatcgctg cggcgcggtg aaagaagagt gtgcagaagg cgtggaagac
    17881 attatgcata cgctggcggc aaaaatgggg tttgccctgc ggcataatgt gattgaagca
    17941 catgggctct gtgcggcatg cgtggaagtg gaagcgtgtc gtcatcctga acagtgccag
    18001 catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaag agggcgtaca tccttgtaca
    18061 cgtcgggcag gagggattaa ttaccagcga tattcattgc gggtttccca atccacgacc
    18121 tctttttctg cctgatcttt ctgataacca taacgttcct ggattttacc gaccagttga
    18181 tcacgtttac cttcaatgat cgtcatatca tcatcggtca gtttgcccca ttgctctttc
    18241 actttacctt taaactgttt ccagttaccg ccggcttcat ctttattcat aatcaagacc
    18301 tcatcgttag gttgtgaatg agagtacgtt cacttttctt ctgaacgtga gattaagtgt
    18361 agtcagcaat ttggcttagg ataatttatt cagaattttt aaccgtcacg ttgcggcaaa
    18421 ccaggtaccg ttacgccagt gacgccgcca gatagccgcc agagaaagcc cgcgcaacgc
    18481 cagaaagacg gttagtgcca gccacaaacc atgattaccc agccacggca gcgtaaggag
    18541 cgtcagcgca aaacctgcgg cggccaccgc catactgtta cgcatttcgg cggcacgcgt
    18601 tgcgcctata aacatgccgt ccagcagata acaccagacg ccaaccaacg gcaaaatcac
    18661 ctgccagata agatagcggt cagccagctg ctgaatctgg gttaacgacg tcagcaacgc
    18721 aatgatgtgt tcccctgcca gcaaataaac caccgaaaat agtaacgcta cgatccccga
    18781 ctggcggcac gctgcccgcc agacatccag caactggcta ccgtcgcgcg caccgtatgc
    18841 ctgaccggaa tgtgcttcaa ccgcgtaggc aaaaccatcc agcgcatagg cggtaaaggt
    18901 gagtagcgtc atcagaaccg cgtttacagc gataatgtca ctccccagtc gcgcgccaag
    18961 tacggtgatc gcgccgaaac agagttgcaa caacagcgaa cgcagcatga tatcgcggtt
    19021 aagcgccagc aagcgacgga agtttcctcg ccaggcagtt ttcgacattt cgccggaaat
    19081 tccgcgtagt ttgaggattt tacgcaccat tagcagacca atcagcaatg ttgcatattc
    19141 cgcaataacc gtcgccagag ccgcgccctg cacgttcata tgcagcccca tcactagcca
    19201 gacatccagc acaatgttga ggatattgcc gaccactaac aaaattactg gcgcacgggc
    19261 atattgcacg ccgagtaacc aaccgagtaa taccagattc gccagcgacg ccggtgcgct
    19321 taaccagcgg atttcaagaa agcgccgcgc ctgttccagg actgcttcac taccgccaac
    19381 aatatgcaga gccagatcga taatcggtgt acgcagcagc gcaattaacg ccccagcccc
    19441 caacgccaac agcaacggtt gcaccagcgc acgggctaat gcctgaggat ttttggcacc
    19501 ataagcctgc gcagtcagcc cggtggtgct catgcgtaaa aacagcaaca gcataaagag
    19561 aaagctggtc gccgttgcac caaccgccac gccgcccaaa taaaccgggc tatcaagatg
    19621 accaattacc gccgtatcga ccagtcccag caacggaacg gtgatattgg agaaaatcat
    19681 gggtaaggcg agatgccaga gtgctttatc agatgaagtg aggaatgcca tgcagacaag
    19741 cctgatgaag agagatgaaa aacaaaccgc gacgcctggc ggcatcgcgg tctcagagat
    19801 atgttacagc cagtcgccgt tgcgaataac cccaaccgcc agcccttcaa tggtgaagct
    19861 ctgctgacga aggtcaacga caattggttt aaactcgcta ttttctggca acagttcgac
    19921 tttattaccc tgttttttca ggcgcttaac ggtaacttcg tcatcaatac gtgcgacaac
    19981 gacctgaccg ttacgtacat cctgagtttt atgcactgcc agcaagtcac catccataat
    20041 gccgatatct ttcatcgaca tcccgctgac gcgcagcagg aaatcagcat tcggcttgaa
    20101 caaggaagga tcgacctgat aatgaccttc aatatgctgc tgcgccagaa gcggttcacc
    20161 ggcagccaca cgacctacca gcggcaaccc ttcttcctct tcctgcaaca gacgaatccc
    20221 gcgtgatgcg ccggaaacaa tttcaataac gcctttgcgt gccagcgcct tcagatgttc
    20281 ttcagccgcg tttggggaac ggaaccccaa acgctgcgcg atttccgcac gcgtcggcgg
    20341 catacctgtc tggctgatgt gatcacggat gagatcaaac acctcttgtt gcctggccgt
    20401 taacgctttc attccgcccc ctgggtgtat atacagttat gctgtgagta tatacagcaa
    20461 aaggcgattt tggaaccata aactgcacaa taaaccagag atttatcgaa atctggaagg
    20521 gttatccaaa atgcgaccat aacaggatgc accaggttat cacggcgacg ataatcgcaa
    20581 tcagcaccgc ggcggatccc atatctttag cgcgtccgga aagctcatgg tattcagagc
    20641 caattcggtc aaccactgct tcgatggcgc tattgagaat ttccacaatc atcaccagca
    20701 tcacggagct gataagcagc acgcgggtaa ttgcgtccac atccagccag caggcgatga
    20761 ccaccgccaa caataccgct acgccttcct gacggaatgc cgcttcgttg atccatgcag
    20821 cgcgtaaacc tttccaggaa tagccagcag ctttgataat tcgggtgaat ccagtggtat
    20881 tattggccat tgaaagaacc tttttacatt atgagcgtca atatcagtgt accgttaaat
    20941 ccgatgattc gggtttgcgt aacgccacgc gaaacatgag cggataccac agaatttccc
    21001 atgactttct gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt
    21061 atgtccggct ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta
    21121 aaaagcaagt ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca
    21181 attatgtacg ttttaccgta caactcgaaa gcagatttgc tgacgttacg cgcccagtgt
    21241 ctggctcatg acttgcctga cccgttagag ccgctagaaa tcgacggcac gctactgccg
    21301 cgctatgtgt tcattcacgg cgggccgcgt gtattcacct attacacgcc gaaagaagag
    21361 tctattaagc tgttccacga ctatctcgat ttgcaccgta gcaacccaaa tctggatgtg
    21421 cagatggtgc cagtatcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagta
    21481 aacccgccgc tgcgtatgct taacggcgta cagaaatttt tcgccgtact gtggctcggt
    21541 cgcgacagtt ttgtgcgttt ctcgccgtca gtttcgctgc gccgtatggc ggatgaacac
    21601 ggcacggata aaactatcgc acagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt
    21661 caacgtctgg ctgccgtagg cccacgtctg cctgctcgtc aggatctgtt taataagctg
    21721 ctcgcctccc gcgccattgc caaagcggta gaagatgaag cgcgcagcaa aaaaatctcc
    21781 catgaaaaag cgcagcagaa cgcgattgcg ctgatggaag agattgcggc gaatttctct
    21841 tacgagatga tccgcctgac tgaccgcatt ctgggcttca cctggaaccg actttatcag
    21901 ggcatcaacg tccataacgc cgagcgcgtt cgccagctgg cccacgacgg tcatgagctg
    21961 gtgtatgtgc cttgccaccg cagtcacatg gactacctgc tactttctta cgtgctgtac
    22021 caccaggggc tggtgccgcc gcatatcgcc gccgggatca acctgaactt ctggccggcc
    22081 gggccgattt tccgccgtct gggggcgttc tttattcgcc gtacttttaa aggcaataaa
    22141 ctttattcca ccgttttccg tgagtatctc ggcgaactgt tcagccgtgg ttattccgtc
    22201 gagtacttcg tggaaggcgg tcgttcccgc acggggcgtt tgctggatcc gaaaaccggt
    22261 acgctgtcga tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt
    22321 ccgatctata tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc
    22381 ggcgcgacga aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt
    22441 aatctcggtc agggttacgt caacttcggt gaaccaatgc cgttgatgac ctaccttaac
    22501 cagcacgtac cagactggcg tgaatctatc gatcccatcg aagcggtgcg tccggcctgg
    22561 ttaacgccga cggtcaataa tattgctgcc gatctgatgg tacgcattaa caacgcaggc
    22621 gcggcaaacg ccatgaacct gtgttgtact gcgcttctgg catcacgtca gcgctcactc
    22681 acccgcgagc agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtacct
    22741 tactccacgg actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg
    22801 caaatgaaca agtttgaagt cgagaaagac actatcggcg acatcatcat tctgccgcgc
    22861 gagcaagcgg tgctgatgac ctactatcgc aacaacattg cgcatatgtt ggtgctgcct
    22921 tcgctgatgg cggcaattgt cacccagcat cgccacatct cccgcgacgt attgatggag
    22981 cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt tcctgcgctg ggatcgcgac
    23041 gagttgccgg acgttattga tgcgctggca aatgagatgc aacgtcaggg gctgattacc
    23101 ctgcaagatg atgagttgca tatcaacccg gcgcattctc gccccctaca gctgctggcc
    23161 gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac
    23221 ccgtcgatca accgcggtac gctggagaaa gagagccgca ccgttgcgca acgtctctcc
    23281 gtgctgcacg gcatcaacgc gccggagttc ttcgacaagg cggtgttcag ttctctggtg
    23341 ctgacgctgc gtgatgaagg gtatatcagc gatagcggcg acgccgaacc ggcagaaacg
    23401 atgaaggttt atcagttgct ggcggagttg attacatcag acgtgcgttt gacgattgag
    23461 agtgcgacgc agggcgaagg gtaatcagag agaattgccg gatgcggcga aaacgcctta
    23521 tccggccttc cataatctgc aaattcaata aattgcgatg tgttgtgtag gcctgagaag
    23581 cgcagcgcat caggcaaccc agaagaaagc cggatgatca tccggctttt ttacatcatc
    23641 agaaatgcca gtaactcatt gccagtccta aaaacagtac cagaccaaca tagttattat
    23701 tcataaatgc tttaaagcag gcttcacgct cgcggttggc aatcagtttt tgttgataaa
    23761 caaacagcgc gccagccacc agaattgacc agtaatatcc ccagcctaag ccatttaact
    23821 caccgatgat cgccatcagt gccagtacgc caatctgcaa aataccaata atcaatttat
    23881 cgtattggcc gaacaggatt gcggtggatt taataccaat cttcacatca tcgtcacggt
    23941 caaccattgc atactgcgtg tcgtaagcca ccgcccagag aatattggcg aggaacatta
    24001 accagcaact caatggcacc gactcactca cagcggcaaa agccattgga atcgaccagc
    24061 caaacgccgc gcccagcacc acttgcggga gatgggtata ccgcttcata aacgggtaca
    24121 cccacgccaa cgccagagcg gcaatcgaca acagaatggt catcgtattc agcgtcagca
    24181 ccagtaaaaa tgaaatcaat accagcacta caaacagcgc gcgcgcctct ttctccgtta
    24241 ccgcgccgct gggaagcggt cggttcgccg tgcgcttaac atgaccatca aacttgcggt
    24301 cagcataatc attcaccaca catccggcag cgcgcatcag ccagacaccc gcgacaaaca
    24361 ccgccaggat ccagagctgg ggaacgcccg gtgtcgccac ccacaacgcc cataatgttg
    24421 gccagagcag cagtaacgcg ccaattggct tatccgtacg cattaagcga tgaaacgcca
    24481 gcagcttatt ctgcgtcaga ctccactcca tatttttttc ctcttagtac aacggtgacg
    24541 ccggtaaaaa cagttctgtt agcaacagcg gtttaccgct taatcgcagg cgggaacgtc
    24601 gcccccacag tccggcatca cggcctatct caataaagtc ccgggttaat gtcgatgatg
    24661 tgaacagata gcgtcctagc ggcgttttac ccaatttttg taacgccagc tccggcccgc
    24721 ttaacgttga cacaggaacg acggtacgac cggcaagcca cggttcacca tcggcacata
    24781 acaaaatttc acgtaaccag taacgagact ctttcggcag cagcggcagt tcttcgggga
    24841 tttcattctg ctcgacaaac ccttcgcgga tcatcgtcac gctcaccgtt tttccctgct
    24901 gttcaaaacg ttttgtcatg gaatcttcca gcaacagcca gtcgagcagt tgcgaatcca
    24961 gggcagggat ctctttaaaa tagcgcagcg cacgcagttg cgttaacgcg gggtgtgaca
    25021 tgccgaactc tccgttacat aacgtaaagg cattgtatcg cagaaaagtg tttacgggag
    25081 atgtaaagca aattaagtga tctaatgcgc aacaaaaacg caacaatcgc atgaagtggt
    25141 aaaacgctgt tgaaccttac gctttgccga tgcccgtgct ggcgagggca accgtagggg
    25201 catcaccgcc catgatgcca ggaacggtga tgttttcctt tacttaaata cctatattct
    25261 ttatacgagt agctcgccaa ctcgcagtaa aaattgggaa aagcgcatat ttaccatgag
    25321 atttggagct aaaaagtgtt tcctgagtaa gattaatagg caacatctta tgtctggatt
    25381 tatagataca cgtggaattc caattggtat cttttgtagg attcaacata tc
//
LOCUS       DAJVXP010000052        25333 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVXP010000052 DAJVXP010000000
VERSION     DAJVXP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.052"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1254)
                     /locus_tag="OQS10_002027"
     CDS             complement(<1..1254)
                     /locus_tag="OQS10_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS2293056.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPR"
     gene            complement(1257..2924)
                     /locus_tag="OQS10_002028"
     CDS             complement(1257..2924)
                     /locus_tag="OQS10_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS2293057.1"
                     /translation="MSATRIQAVYRDTGGEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(2921..5029)
                     /locus_tag="OQS10_002029"
     CDS             complement(2921..5029)
                     /locus_tag="OQS10_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS2293058.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(5016..5837)
                     /locus_tag="OQS10_002030"
     CDS             complement(5016..5837)
                     /locus_tag="OQS10_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS2293059.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(5997..7826)
                     /gene="glmS"
                     /locus_tag="OQS10_002031"
     CDS             complement(5997..7826)
                     /gene="glmS"
                     /locus_tag="OQS10_002031"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS2293060.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(7988..9358)
                     /gene="glmU"
                     /locus_tag="OQS10_002032"
     CDS             complement(7988..9358)
                     /gene="glmU"
                     /locus_tag="OQS10_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS2293061.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(9709..10128)
                     /gene="atpC"
                     /locus_tag="OQS10_002033"
     CDS             complement(9709..10128)
                     /gene="atpC"
                     /locus_tag="OQS10_002033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS2293062.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(10149..11531)
                     /gene="atpD"
                     /locus_tag="OQS10_002034"
     CDS             complement(10149..11531)
                     /gene="atpD"
                     /locus_tag="OQS10_002034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS2293063.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(11558..12421)
                     /gene="atpG"
                     /locus_tag="OQS10_002035"
     CDS             complement(11558..12421)
                     /gene="atpG"
                     /locus_tag="OQS10_002035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS2293064.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(12472..14013)
                     /gene="atpA"
                     /locus_tag="OQS10_002036"
     CDS             complement(12472..14013)
                     /gene="atpA"
                     /locus_tag="OQS10_002036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS2293065.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(14026..14559)
                     /gene="atpH"
                     /locus_tag="OQS10_002037"
     CDS             complement(14026..14559)
                     /gene="atpH"
                     /locus_tag="OQS10_002037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS2293066.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(14574..15044)
                     /gene="atpF"
                     /locus_tag="OQS10_002038"
     CDS             complement(14574..15044)
                     /gene="atpF"
                     /locus_tag="OQS10_002038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS2293067.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(15106..15345)
                     /gene="atpE"
                     /locus_tag="OQS10_002039"
     CDS             complement(15106..15345)
                     /gene="atpE"
                     /locus_tag="OQS10_002039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS2293068.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(15392..16207)
                     /gene="atpB"
                     /locus_tag="OQS10_002040"
     CDS             complement(15392..16207)
                     /gene="atpB"
                     /locus_tag="OQS10_002040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS2293069.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(16216..16596)
                     /gene="atpI"
                     /locus_tag="OQS10_002041"
     CDS             complement(16216..16596)
                     /gene="atpI"
                     /locus_tag="OQS10_002041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS2293070.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(17213..17836)
                     /gene="rsmG"
                     /locus_tag="OQS10_002042"
     CDS             complement(17213..17836)
                     /gene="rsmG"
                     /locus_tag="OQS10_002042"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS2293071.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(17900..19789)
                     /gene="mnmG"
                     /locus_tag="OQS10_002043"
     CDS             complement(17900..19789)
                     /gene="mnmG"
                     /locus_tag="OQS10_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS2293072.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(20168..20611)
                     /gene="mioC"
                     /locus_tag="OQS10_002044"
     CDS             complement(20168..20611)
                     /gene="mioC"
                     /locus_tag="OQS10_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS2293073.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(20701..21159)
                     /gene="asnC"
                     /locus_tag="OQS10_002045"
     CDS             complement(20701..21159)
                     /gene="asnC"
                     /locus_tag="OQS10_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS2293074.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            21311..22303
                     /gene="asnA"
                     /locus_tag="OQS10_002046"
     CDS             21311..22303
                     /gene="asnA"
                     /locus_tag="OQS10_002046"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS2293075.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(22308..23759)
                     /gene="viaA"
                     /locus_tag="OQS10_002047"
     CDS             complement(22308..23759)
                     /gene="viaA"
                     /locus_tag="OQS10_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS2293076.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(23753..25249)
                     /gene="ravA"
                     /locus_tag="OQS10_002048"
     CDS             complement(23753..25249)
                     /gene="ravA"
                     /locus_tag="OQS10_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS2293077.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6279 a         6567 c         6002 g         6485 t
ORIGIN      
        1 tcttgggtga tcaaggctac tatctagacg ctttatgatt cttaatagtt gtcgtacagc
       61 aattcgatct ctttggttcc aatcaactct aggggcgggt gccagacgct cttgttgatg
      121 ctgttgattc cagtgaacta gccaatccct gtcatgtcgg taaagccaag cgtatagcac
      181 cccaccctct aaagactgtc ttgccgcctt aattccttgg tatttatgca ctagttgctg
      241 ccagtcttta tgcttaacag ataaatcttc agagttaggt tgcacagact ggctaacagg
      301 tcttgtcgtt atagagtgct cagtaagagc acttgcctgc tgtagcgctt ctataaccgt
      361 tagttttggc aataaggctt gccacacaat actatgctgt aaataactaa aggcttttct
      421 atgcttacgg aatatacttt tcagccaaca cgtgtccttg ttctctgcca actttaaatc
      481 cagtttctct agtgcctcat cactaaaagt ctgcctcact ctctccgcca ccaagtcatg
      541 acgaatgtgc ttgcttttgg ttagccctag atcctgcgct aagcgctgat aaaacagcgt
      601 ccactgctca aggcttgggg aaagctcttg cgctcgtgga gcatctaaca gaggggctat
      661 ataagcagct agtgctgtta attgagatag ggagtctttg gggtagtctg aaagcagctc
      721 agtatgaccc aaagcccaaa attgatgtcg gtgatcatct acagctctat caaagaagac
      781 taaagcaccg tgttttggac aatatggcaa agcgggcaaa taccaatctc gttgccaaaa
      841 ggcttcccca tacctattta gctgaagagc aacgcaatca gggcagtatc taaagcggtt
      901 atcgctctta actctagaag cagcgactcc tagcattaaa tgcaccgcac cttgcgcttg
      961 gtactccatt aaccgaatag cttcgtctcg gcgctccttg cctacaaacg gagcatataa
     1021 agggaataag gtatgctcat aaataagctg ctgaacagcg taacgtcctg tttgatgtag
     1081 atgtcttgct atcacaccta aatgcgaggg cagacctaag gtagcgacca ccttgcggtt
     1141 gccatacacc tcatccaaca gctgcttagg actaacaatc ccttgataaa cgcctgcccg
     1201 tgcaatagtg ctataaatca gctcattcga gtacggaaca ggaaagtttc tcatggctaa
     1261 cctgcttgtt taaataagct cgccatatcc actattaccc ctttcccttt taaccgttca
     1321 tgcatggttt tttcaggttg gcgttgtgaa tagatataac gtaaatccgt atcaggcaag
     1381 ctatcccagt cgctgggctt gactaccttt atagccgaaa cctttttact cttggagggc
     1441 ttttctagtt ctgacactac cgtttcgcct tccatcaacc actgcaaaac aagaggcagt
     1501 aacttttgtc ttgtcatcgt tggattctgg ctaaacgctt ttttaatagt gggaattaac
     1561 aggcttgaat cgtaatcctc tttcagcatc agatataaat gacgctgatc ttcggtatct
     1621 aactcttgaa gggctttttc ttctggtgtt tgttcttgta tcgctgcgat atctagctga
     1681 agttggatta accgtttatc aatctcggga acgactagat cagaataacg agcaatgcgt
     1741 tctgggatac ccgagcgtaa tgcctctagc atggggtgca caggctttaa ctcatcttga
     1801 tacacttgcc gcaataaacc agcggtaata cgctcattgc ctaaagctag cgcacggagc
     1861 tgagcgagta caaaaagttt tactacaatg tccatcactc cttggcttag ctcataccac
     1921 acatcacgga cctcatccga taacagcgca tctttgcgtt gtaaaagctg taattgccaa
     1981 agattatccg taaaagcgat ccactcttga ttgggctttc cacgttgcgt ttgttgtata
     2041 ggatcccaga atatagctcc aaaccctgcc cctctacgtg cagaccgcaa atcagcctca
     2101 aaaatctctc gtgctttagg ggtaccaatc aacatcactg gtacgccaat aatattcacc
     2161 atcgtcacaa aaaagttcag catctcttga gatccacccg aacgagagcg gcttaaatgc
     2221 tgaatttcat caataaccaa caaccctaaa gcatgtgcat tggctatttg cgacatcaaa
     2281 gccaacatgg tttctatacc atgacgtttt aagccataac gacgctcata gttcgagccc
     2341 aaggctcgat ccaacgctct gaaaaaattc aagcagattt cttttagcga accattatgc
     2401 gagcagtcta ttttcaaata caccacctgc tctacattga gttcacgatg gtaaatcacc
     2461 tgaggatacg tggctagaat acgatgaaga gaggtcgtct tcccactacc agaacaacca
     2521 attaacaata agctttgtgc cgtagatcgt gcctcctcaa agcgaaatgt ctccaactct
     2581 cccgtttgaa cacgctcata accattttgt aaatgctttt gtaaatctcc tgttttagga
     2641 tttctgccta cgtagccacc tcgaatcatg accgaaatac gctcacttag tagcaaatgc
     2701 gtacctaatg gctgaaaata gtcatctgga atacgacaaa tggtatgagc tctgataaca
     2761 cgggactttt gcaagtcaga ggaagtaagc tgtaaagagg atttcagtga tgcagcacta
     2821 ttcactgact cttgtaatgg tggtaaggcc tcgataaaag gattatcacg ataagcctct
     2881 ccccccgtat cacgataaac tgcttgaatc cgggtagcac tcatgactca tccttttctg
     2941 gtggatcctg aaataattca ggcacgtatg tgggtaggct gtaatcttct tgatcatccg
     3001 cttccactgc gttgaaagga ataactttag cctcatcacc tgaagagctt ggcttcaaat
     3061 gctccgcacg ttttttacgc tccgaggtca cggcttcttt tttattagtt ttaatctgct
     3121 taatgcgtgt tgatttgggc tcagtagtac tgggcgttaa cttattcgct ttctgaatgg
     3181 tttgctgaat aaacgcctca agctccctgc gtttagttaa ctcatcctgc ttcgcattgg
     3241 ctttattgtg tttttcttgt gcttgtatat cccaaacctc ccaaaatgag agacctttaa
     3301 actgccgact acgttccgtc agattacagc gccaaaatac acggctgcca acttgcggaa
     3361 acaaataaat cgtatcaacc agcactgggt cataagccgc ttctaaatgt tgaggtctag
     3421 ctatatcagt gctccgctgc aaccaaccct cacgcagaat ctctgacccc gagtaataca
     3481 aaccccacaa attaacgcca aatgaagaaa tagagacctt tcggcgaggc agtaacgcta
     3541 ctcgcaactg ctcttgctcc acagccctta aactacctgt acgatgctgc ataccccatt
     3601 gccatagctg gacaggaata gacggtaaat ctgtaggaaa atcagcatct cgatcgtatt
     3661 tatccatcac cagatggtta tttctgaata agatcgtacg caaaataatt tgtgtgaact
     3721 caaataccga cagagatgca tctaacctat agtctgtttc accatggctt ttgatccgac
     3781 tgccctctac aatgccaggt gcaaaggact taaactcggc ttgtagtgtt ctaaaagtgc
     3841 tttccactat gcctttagca tcgccacgtc taggtggagc actttccact cgcacattaa
     3901 aactagaaac taaggcttcg acctgatgac tcattaattc gccacggtcc gctagcaaca
     3961 catctggcaa acctacacac ggccagtctg agctactaat ctcaatatca tgctgggcac
     4021 aaatggccgt tttgtcagag caagcattta caaaagcctg catcgccacc acataagacg
     4081 gattttcaaa gccgatataa aagcccgtga tcatccgact aaacacatca atcacaatgt
     4141 aaagcgttgg tcttcctatg attttttggc gatcatgatg atccactaaa taaatatcag
     4201 caatcgtggc atcaatctca taacgactcc cagggcctaa cgcctgagaa gtggctgtac
     4261 tacttaaggg tcgtacgtct tttttatata cccctgcttt aactctagac tttaagcgct
     4321 gagctttagg gtattctcga tcataaaaat aacgaaactg acgtagtgtt gggtaatcct
     4381 cttggggaat gcgaggaaaa tactgagcaa acaagtccac aaatcgtcta taggcaacgg
     4441 tggtctttgt acctttttga tttaacaggt gcttttctat ggtcaaccta aaaaggcgtt
     4501 caatctcggg cgttaccttg gttccttcac ccttaccata ttctctggct cggccaatct
     4561 ttgctgttcc tgtcgctgaa cgtctttccc ctggtgcacc gctgtttttg tagtcaggaa
     4621 ttaatgcatt aggcgtttga ccacgctgcc agtaacggcg taacaactta taaactgtag
     4681 ccttagtaac cttatgttct tggaccacat gctcaacgag ttcgcttctg actttagggt
     4741 cgaaacgatc cttactatta ataataggaa gaatttttcg ataatcctcc tcgcgcttct
     4801 ggaagctgac agaatctaca gacggctctt ctaaatcaag atgtacataa gggtcatcag
     4861 ttctaactaa gcggccttca tctaaatact gcattagcaa caactcagcc ctagcttgtg
     4921 gaactccttt atcagcgctt atttgcatcc agacaacttg gccatcctct atagccaaaa
     4981 tgcgatacgg atcattatca aatagcacaa cctcattaat ttgccacata gcgcaactcc
     5041 tccatattca ctacttggct aatacagaga tctgcacact tatttgccct gaaagactta
     5101 taaatattga acttaataaa accatttgcg gttaaggctc gtatctcact caatgttttg
     5161 cctaactcca aatcataagc aatatcaacc tgcttacaga cattgataat gttttcatct
     5221 cctttttctt gcaggatatg ggccaatggg gatagttgtg ctaaaagctc cgcagaaact
     5281 tcttctgttt tcactgaata aagccattca atattttctt ttactacggg atttatttct
     5341 ttatcagtaa aaatgaacca aggaatttgc ttttgctgcc aatagcgacg ctctagttct
     5401 agtttttcta aggtacgctc gtcttgtaag gctgctgcag gtttgacttg aatagcaaac
     5461 tgctcaaaag gaccatcttt gcagtccact aaaaaatcag tagacataac ctgatctaca
     5521 ccacgaataa caggatgctt aataccacta tctattgcaa tctgcctggt atcactaggt
     5581 aataagggga actgctcgcg tatatctagc acgctgctct cccactcaag actgagaaaa
     5641 acagcaagct ctaagtcaga tagcaaatga tggactcgtc ccgtcttatg agaataaata
     5701 cggtgggaac gacctgaaga aggaacttct tgtactgtta gccatggaat atagtcttta
     5761 ccatgccctt ggccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
     5821 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
     5881 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
     5941 gttcagttta agactttatt gtccgcccac aagccctttt ttatctatga tagtgattac
     6001 tcaaccgtaa ccgattttgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc
     6061 gcgacatggt aagccagcag ctgcagcgga acggtgtaga agatcggtgc aatcacctct
     6121 tccacatgcg gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga
     6181 tcggcgaaga catacaattg gccgccacgc gcgcgaactt cttcaatgtt ggatttcagt
     6241 ttttccagca attcgttgtt cggtgcaacg acgataaccg gcatatcggc atcaataagc
     6301 gccagcggac cgtgtttcag ttcaccagca gcgtaggctt cagcgtgaat gtaggagatc
     6361 tctttcagct tcaatgcgcc ttccagtgcg attgggtact gatcgccacg gcccaggaac
     6421 agtgcgtgat gtttgtcaga gaaatcttct gccagagctt caatgcgttt gtcctgagat
     6481 agcatctgct caatacggct cggcagcgcc tgcagaccat gcacaatgtc atgttcaatg
     6541 gaggcatcca gacctttcag gcgagacagc ttcgccacca gcatcaacag cacagttaac
     6601 tgagtggtga atgctttagt ggacgccacg ccgatttccg tacccgcgtt ggtcatcagc
     6661 gccagatcgg attcgcgcac cagagaagaa cccggaacgt tacagattgc cagcgaacca
     6721 aggtaaccca gctctttcga cagacgcagg cccgccaggg tatccgcggt ttcgccagac
     6781 tgtgacaagg tgatcatcag gctgttacga cgcacggcag atttacgata gcggaattca
     6841 gaggcgattt cgacgtcgca cggaatacct gccagcgatt caaaccagta gcgggaaacc
     6901 ataccggagt tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc
     6961 agttcgtcgg cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg
     7021 gtaagggtgt tttttatcgc gttcggctgt tcgtagatct ctttctgcat gtagtgacgg
     7081 taaatacctt tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact
     7141 tccgcgccag ttttatcgaa gatgtttacc gaacggcgag tgatttccgc aatatcgccc
     7201 tcttcaagga agataaagcg acgggtcacc ggcaacagcg ccagctggtc agaagcgata
     7261 aagttttcgc ccatacccag gccaatcacc agcggactac cagaacgtgc cgccagcagg
     7321 gtatccgggt gacgggagtc catgatcact gtaccgtacg caccgcgcag ctgcgggata
     7381 gcacgcagaa cagcctcacg cagagtcccg ccttgtttca gctcccagtt caccagatgg
     7441 gcaatcactt cggtgtcggt ttcagaaacg aaggtatagc cacgcgcttt taactcttca
     7501 cgcagcggtt catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca
     7561 tgcggatgcg cgttagcttc tgaaggttca ccgtgggtcg cccagcgagt gtgagcaata
     7621 ccggtgccgc catccagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg
     7681 aggcgacgca ggcgggtcat atgaccttcc gcatcaacaa cggccagacc ggcagagtca
     7741 tatccgcggt attccagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc
     7801 gcgatcgcgc caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca
     7861 acctgtatgc ccgttttttc gggcgccccg agccttgtag agagtggggt tatttttata
     7921 gttactgctt gtgggaggga gattatgtta tctcctcatc ccagatgacc ggattacccg
     7981 gccagaatta ctttttcttt accggacgac gccagccttc tttctgagtc tgcggcacac
     8041 ggctgatagc taatgcattt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg
     8101 tcgcgccttt gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat
     8161 cgtcgccgat aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg
     8221 cgccgatgtt aacgttatcg ccaatttccg catcgcccag gtaagtcaga tgcccgactt
     8281 tcgagccttt acccagacgc gcttttttca tctcaacgaa gttaccaacg tgtgcacctt
     8341 ccagcaactc agcaccagga cgcaaacgag caaacgggcc aatagtacag gccgctgcca
     8401 gattcgcgtc ttccacaacg gtatacggac tgatttcgca atcatcgcca atcacgctgt
     8461 ttttaatcac gcaaccggtg ccgattttca cgcgatgacc gagagtcacg ttgccctcga
     8521 tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa
     8581 aacgcgccgg atcgcgcagc ataacgcctg ctaacagcag tttttcagcc tgttcggact
     8641 ggtaaacgcg ctccagacgg gagagttgca ggcggttatt cacaccttct acttcgctta
     8701 aacgttgcgg atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt
     8761 cggtgatgta gtattcgccc tgagcattat tgttggtcag cttcgccagc cagcgtttca
     8821 tatctgcgcc gttagcgatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt
     8881 cggtggcatc tttgtgctca acaatgccgg taactttgcc attttcacgg gtgatacgtc
     8941 cataaccggt cggatcatcc agtttcaccg tcagcagacc aatgccaccc tgcggtttag
     9001 catcacgcag gcgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta
     9061 aaatgtcttc atcatcggca aagaaaggtg cggcctgctg cattgcatga cccgtaccca
     9121 gctgctctgc ctgaagcacc cagttaaggt tgtcgtcttt cagcgcctgt ttaagcagat
     9181 cgccgccgtg accgtacacc aagtgaacgt gcgctgcgcc taattcattc gcagcatcaa
     9241 tgacatgctg aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat
     9301 acatgcgcgt gcctttgcct gcggcaagga tcactacgct catagcatta ttcaacatac
     9361 gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt
     9421 ttttcatcgt gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga
     9481 aagtgccatt ttcgaccatc gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc
     9541 atttaagcgt caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca
     9601 ggggtgtttt atatcagcag gatctatgtg aacgctcttc aggacggatc acgcgtaaaa
     9661 aaaagccagc ctgtttccag actggctttt gtgcttttca agccggtgtt acatcgcttt
     9721 tttggtcaac tcgataacgc gcagctgcgc gatcgctttg gccagttccg cagacgcctg
     9781 agcgtaatct acgtcgccgt gagagctgct aatgtgctct tcagccttac gtttcgcttc
     9841 catggctcgc gcttcgtcga gatcctggcc gcgaattgcg gtgtcggcca gaacggtcac
     9901 gttgccaggc tgcacttcaa gaatgccgcc agacagatag ataaactctt cgtgaccgtg
     9961 ctgtttcacg atgcgaatca taccaggctt aatggcggtg agcagcggtg cgtggccagg
    10021 gtagatcccc agttcacctt cgctacccgt tacctggatt ttctcgacca gaccagagaa
    10081 catttgttgc tctgcgctga cgacgtccag gtggtaagtc attgccatat caccctccga
    10141 ttaaggcgtt aaagtttttt ggctttttcc acagcttctt cgatggaacc gaccatgtag
    10201 aacgcctgct ccggcaggtg atcgtattcg ccttccatga tgcctttaaa gccacggatg
    10261 gtgtctttca gggagacgta tttacccgga gaaccggtga atacttctgc cacgaagaac
    10321 ggctgggaca ggaagcgctg gatcttacga gcacgcgcta ccaccagttt gtcttcttca
    10381 gacagttcat ccatacccaa gatggcgatg atgtctttca gttcctgata acgttgcagg
    10441 atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag cgggtccagc
    10501 tgacggctgg tggagtccag cgggtcaacg gccgggtaga tacccagaga cgcgatctga
    10561 cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga cgggtcagtc
    10621 aagtcatccg caggtacgta taccgcctgt acggaggtga tagaaccagt tttggtggag
    10681 gtgatacgtt cctgcagaac gcccatctct tccgccaggg tcggctgata acctaccgct
    10741 gaaggcatac ggcccagcag tgcggatact tccgtaccgg ccagggtgta acgatagatg
    10801 ttgtcaacga acagcagaac gtcacgacct tcgtcacgga atttctcagc catggtcaga
    10861 ccggtcagag caacgcgcag acggtttccc ggcggctcgt tcatctggcc atacaccagg
    10921 gatactttgt cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt accctcacga
    10981 gtacgttcac ctacgcccgc aaacacagag taaccggagt gctcgatcgc gatgttacga
    11041 atgagctcca tcatgtttac ggttttacct acacccgcac caccgaacag accaacttta
    11101 ccgcccttag cgaacggaca catcaggtcg ataactttga taccggtttc cagcagttcc
    11161 tgagagtttg acagctcttc gtaggaaggt gctgcgcggt gaatcgccca acgctcttct
    11221 tcaccgatct cgcctttcat gtcgaccggt tcacccagta cgttcatgat acggcccaga
    11281 gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt cttttacatc cagaccgcga
    11341 cgcagaccgt cggaggaacc cattgcgatg gtacgcacga taccgccgcc gagctgctgc
    11401 tgaacttcca gcaccagacg ctcattacca ttttgcacct caagagcatc gtacacgcgc
    11461 ggtacggcat cctgagggaa ttcgacgtca actacggcgc cgattacctg gacaatcttt
    11521 ccagtagcca tcttaaatcc tctacgaaat aacctgttta aaccgcggcg gcccccgaga
    11581 cgatctcggt gagttcctga gtaatgctgg cctgacgagc tttgttgtat accaactgca
    11641 gctctttaat caggctgccg ccattgtcgg tcgcggcttt catcgccacc atacgggcgg
    11701 cctgctcgct ggccaggttt tcaaccacgc cctgataaac ctgagattcg acataacgac
    11761 gcagcagggt atccagcaac gccttcggat cgggttcgta caggtaatcc caggatttat
    11821 gtttcagatc atcatcatct gatgccggta acggcagcag ctggctgatg gtcggaacct
    11881 gagacatggt gttaataaat ttgttgctga caatgtaaag cttgtccaga cggccttcgt
    11941 cgtaagcctg caacatcact tttaccggac cgatcagttc ggacagggaa gggttatccc
    12001 ccatgccggt gacctgggca acaacattgc cgcccacgga gttgaagaac gacacgcctt
    12061 tcgagccgat cattgcgagg tcgcattgaa cgcttttgtc ggtccaggtc ttcatttccg
    12121 ccagtagttt tttgaacagg ttaatgttca aaccaccgca caaaccacgg tcggtcgaca
    12181 ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag gtaagggtgc ttatattcca
    12241 gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt ttctgcataa ggacggctgg
    12301 ccgccatgcg atcctgcgat ttacgcattt tggaagcggc gaccatctcc atcgctttag
    12361 tgatcttttg cgtgttctgg acgcttgcga tcttactacg tatctctttt gcgccggcca
    12421 tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac gttaccagga
    12481 ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta
    12541 gccaccggtc tggttgatct cttgcatcaa cggagcgtga tcacggtcga cgtaagccag
    12601 cagagcggct tcgaagctgc caattttcga cagttcaaca tccgccaggt aaccacgttc
    12661 tgctgcgaac agaaccagag actgctgcgc aacggacatc ggcgcatact gtttctgttt
    12721 cagcagttcg gtcactttct gaccgtggtc aagctgctta cgtgttgcat cgtcaaggtc
    12781 ggatgcaaac tgagagaacg ctgccagttc acgatactgt gccagagcgg tacggatacc
    12841 accggacagt tttttcatga tcttggtctg tgctgcacca ccaacacggg atacggaaat
    12901 acccgggtta accgcaggac gaataccggc gttgaacagg ttggtttcca ggaagatctg
    12961 accatcggta atggagatta cgttggtcgg aacgaacgca gaaacgtcac ccgcctgagt
    13021 ttcgataatc ggcagtgcgg tcagagaacc ggttttccct ttcacttcac ctttggtgaa
    13081 ggcttcaacg tattcggcgt taacacgtgc agcacgctcc agcagacgag agtggaggta
    13141 gaaaacgtcg cccgggaatg cttcacgtcc tggcggacga cggagcagca gggagatctg
    13201 acggtaagca acagcctgtt tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg
    13261 gtcacggaag tattcgccca ttgcgcaacc ggcatacggt gccaggtatt gcagtgcagc
    13321 ggattcagac gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt gctcttccag
    13381 tttacgtacc acgttagaaa tggtggacgc tttctggccg atagcgacat agatacattt
    13441 gataccggaa tcgcgctggt tgatgatggc atcgatagcc agtgcggttt tacctgtctg
    13501 acggtcaccg atgatcaatt cacgctgacc acgaccgatt gggatcatgg agtcaacggc
    13561 tttataaccg gtctgtaccg gctgatctac ggactgacgt tcgataacgc ccggagcgat
    13621 tgcttctaca gcagagaagc cgtcgtgatc cagcggacct ttaccgtcga ttggtgcacc
    13681 cagagtgtta accacacggc ccagcaggcc acggccaacc ggaacttcca ggatacggcc
    13741 agtacactta actttcatgc cttcggcaag gtcagcgtac ggacccataa caaccgcacc
    13801 tacagagtcg cgctcaaggt tcagtgcgat agcgtaacgg ttacccggca gggagatcat
    13861 ttcaccctgc atacaatcgg ccaggccgtg aatgcggata acaccgtcac ttacagaaac
    13921 aatagtacct tcgttgtgag cttcactcac aacattgaac tgagcaatgc gctgcttgat
    13981 cagttcgctg atttcggtgg aattcagttg catgctccag tccccttaag actgcaagac
    14041 gtctgcaagg cgctcaagac gaccgcgtac gctgccatca atgaccatat cacccgctcg
    14101 gatgataacg cctgccatta cagacttatc gattttgcaa ttcagcttaa ctttgcgtga
    14161 cagacgtttt tccatcgcag cagaaatttt cgcgagctgt tgttcactca gtgcggcagc
    14221 ggaaatgacg tctacctcag cggtagcctc actcacggca cgaaggtgaa taaactgctc
    14281 cagaacatcc gggagcgcgt taagacgacc attttcagcc attacccgaa tcaggttctg
    14341 accgttttcg tccagttgct caccacaaac tgcgataaac gactcggcga gcgtttctgg
    14401 cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt ttggttacct cggcggcaaa
    14461 cgccagcatg tcctgccagc gttctacact ttggtgttcg acggcaaagt caaaagctgc
    14521 tttggcgtag gggcgagcta ccgtaataaa ttcagacatc agcccctccc tccttacagt
    14581 tcagcgacaa gtttatccac gatgtcgctg ttagcagctt catccacgga acgttcgatg
    14641 atcttctcgg cgccagcaac agccaggata gcaacttgct tacgcagctc ttcacgggca
    14701 cgtttacgct cggcttcaat ttctgcctgc gcctgggcca cgattttagt acgttcctgt
    14761 tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc gtttgttcgc ctgctcgatg
    14821 attacctggg cttccgcttt cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg
    14881 tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt
    14941 ttttcgatgg ctgccattaa tggcggccat acgtacttca tgcagaacag aacgaacagg
    15001 acaaacgcga tggcctggcc gaggattgtt gcgttaagat tcacagcaca atgcctctat
    15061 ttagttaacg ttctgatatt gctctttaaa taaaagcaac gcttactacg cgacagcgaa
    15121 catcacgtac agacccagac ctacagcgat catcgggata gcatccacca gacccataac
    15181 gataaagaac tgagtacgca gcagaggaat cagatcaggt tgacgcgctg cgccttccag
    15241 gaatttaccc ccgaggatgc cgataccgat cgcagcaccg attgccgcca gacccatcat
    15301 cacagcggca gccatgtaca gcagatccat attcaggttt tccatgacag tctccagttt
    15361 gtttcagtta aaacgtagta gtgttggtaa attaatgttc ttcagacgcc atcgacagat
    15421 agacgatcgt cagaaccatg aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa
    15481 tggcccacgg cacattcagg atccactgtg accaccacgg caacagacca gcaatcagaa
    15541 tgaaaatcag ctcaccggca tacatgttac cgaacagtcg caaaccgagt gaaactggtt
    15601 tggacagcag gcttacccct tcaaggatta agttgacagg aatgaacgcc cagtgattga
    15661 acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg atgctgtaga
    15721 acagaatcag gataaatacg cccagtgcca tagacagcgt tacgttcacg tccgcagacg
    15781 gaaccacacg cagtgcaggc agacccagta catgttcagc aatgtacggc agcaggtcga
    15841 taggcagtaa atccatcagg ttcatcagga atacccagac gaagatcgtc agggccagcg
    15901 gagcaatcag cttgcttttg ccatggtaca tgtctttcac gctaccatta acaaagccga
    15961 tcaccagctc aatcgcggtc tgaaacttac ctggcacacc gctggtcgcc tttttggcta
    16021 cgctacggaa taaaaccagg aacaacagac ccagcaccac cgagaagaac atggagtcaa
    16081 tattgattgt ccagaaggtg gctggggggt tttgtggatc caccagcgag aatgtacgca
    16141 ggtccagctg aaggttattc aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg
    16201 aagccatgat gccttttacc ctttgttgtt aattacagcc ggtgccagta tctgaaccac
    16261 cagcaccaaa acccacgtaa cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc
    16321 caccaccagt aacaccaaca tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca
    16381 ggccacccgg cctttcgctg gtgtatacgc ctggtgacgc caggcaaata tcataaacaa
    16441 aacgttaggc agaaagactg ccaggccccc gcttattgca gagacgcccc agaaggggtc
    16501 tttgaggctg aacagcaatc cacttgctat caccaccagt aactgaacga gcagaagctt
    16561 ccgagcaacg tttcgactca cgagcgacac agacatcacg tttttcactc ctgctccctt
    16621 cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag catcaaaaag
    16681 cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat aagtagccaa aaggtgaata
    16741 aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt tccgctaacc ctgttcgatc
    16801 atgaaaaact gtaaataacg cgtaaacact agtgataaag cgtgcttcag atcacatatt
    16861 gcgcatgttc gcgcacagta tatttattta cttggcaaat gatgcctttg caagtttatg
    16921 atatttcagt ctaaaaacag atactgtttt aataaatgac atttacacaa caaaaaccac
    16981 ccattgacat ttttaataat gttttaacag ccaatgatgg ttcttagcgc cgatttttag
    17041 cagactgata ttttcactaa tgacttattt tctgcttacc aaaaaaagcc acgttatctt
    17101 gttgatacaa aagagtgaac gtggcgttaa atgtaaccag ttatatcagt agaaaacttg
    17161 gttgttgtta acagtctaac cggtcaattt tttatgattt ttttgataaa aattaaattt
    17221 tatttgcttt aatcaccacc agatgacgtt cgccatccag agctggaacc tgaagtttaa
    17281 ccactgattc gacctgatat tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc
    17341 ctttcagcgc gtagaaacgg ccttgctcac caggaagatg gtggcaccag ctcaccatat
    17401 cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct gaaggaaact
    17461 cttctaccct gctctgtact ggctcaatat tctccagttt aagctcatgt tgcacctgac
    17521 gaaggaaacg cacgcgttta ccaaggctat ccaacagagt gaaatgggct tcaggacgca
    17581 cgatagagag tggaatgcct ggcagtcctg gtccggtgcc gacatcgata aaccgttcac
    17641 cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc atctcattag
    17701 gatcgcggac cgaagtcagg ttgtacgctt tgttccattt atgcagcata ttcacgtagg
    17761 caataagctg gtttttctgg tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg
    17821 agagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcctggtaa gcgggtgctt
    17881 accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt ttcagccaca
    17941 ccagcagaat ggagatagcc gcaggcgtga cgccagaaat acgcgaagcc tggccgatag
    18001 aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg gatacctggc
    18061 ggtaatccag tgtcgcgggt agcagggtgt tctcgttacg cagctgcttt tcgatctcat
    18121 cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt tccgccgcct
    18181 gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca taagtcattt
    18241 ccggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgca gtcaggtgag
    18301 cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc agacgctgac
    18361 gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg tcatccacca
    18421 ggcccagttc acgaccgatt tcagtcaaac gcagatccgc attatcttcg cgcagcatca
    18481 gacgatattc tgcgcgcgaa gtaaacatac gatacggttc tttggttcct aaagtgcaca
    18541 ggtcatcaac cagtacgccg agatacgcct gagaacgcgc cggagcccaa ccttctttgt
    18601 cagcagacag acgggcagcg ttaagaccga ccagcaaacc ttgcgcagcg gcttcttcgt
    18661 aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac ttgctctcca
    18721 gcgtcggttt caggtcgcgt ggatcgaaga agtcatactc aatggcataa cccggacgca
    18781 cgatcttcgc gttttccatc ccctgcatgg agcggacgat ttgcatctgc acatcgaacg
    18841 gcaggctggt ggagataccg ttcggataaa tttcgttaga ggtcagccct tccggttcaa
    18901 ggaagatctg atgctgattt ctgtcggcga agcgcatgac tttgtcttcg atcgatgggc
    18961 agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat tgggctacga tcgaggttac
    19021 tgcggatcac atcatgggtt ttctcgttgg tatgagtgat ataacacggc acctgctggg
    19081 gatgctggga cgcattgccc ataaacgaga ataccggcat tgggttatcg ccatgctgtt
    19141 gcgccagcac actaaagtcg atagttcgag catcaatacg cggtggtgtc ccggttttca
    19201 gacgaccaac gcgcagcggc agttcacgca aacggcgaga aagcggaatg gacggcggat
    19261 caccagcacg accaccgctg taattatcca gaccgatatg aattttaccg tcgaggaacg
    19321 tcccaacggt gagcacgacg gctttggcac ggaacttcag tcccatttgg gtaacagcac
    19381 cgaccacgcg atcgttttcg acaataagat cttcaaccgc ctgctggaag atcatcaggt
    19441 tcggttggtt ctccagcgcc gtacgtaccg cctgacggta gagcacacga tccgcctgag
    19501 ctcgggtagc gcgaaccgcc ggtcctttgc ttgcgtttag tatcctaaac tggatacccg
    19561 cttgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt accagatgtc
    19621 ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg tcgatattgt
    19681 gtgtcaaaag cagagtctgt tgacccatac gcgccgcggc catcgcggcc tcggtgcctg
    19741 catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg gtgattgcct
    19801 cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact caactttgtc
    19861 ggcttgagaa agacctggga tcctgggtat taaaaagaag atctatttat ttagagatct
    19921 gttctattgt gatctcttat taggatcgca ctaccctgtg gataacaagg atccggcttt
    19981 taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat
    20041 aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacc gttgttcctt
    20101 ggataactac cggttgatcc aagcttcctg acagagttat ccacagtaga tcgcacgatc
    20161 tgtatactta tttgagtaaa ttaatccacg atcccagcca ttcttctgcc ggatcttccg
    20221 gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat
    20281 ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa ggtgtcatat tcacgactac
    20341 caataccgat tgcgccaaag cggaccgcag aaagatcggg cttctgttcc tgcaatgctt
    20401 catagaaagg agaaaggttg tccggaatat ctccggcacc gtgggtggag ctgataatca
    20461 gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa
    20521 aacccgcctc ttccagcttt tcagccaggt gttctgctac atattcggca ccgccgaggg
    20581 tgctgccgct gataagagtg atatctgcca taaaccgcca cctttattaa gagtggcgta
    20641 ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta aaaaagccga
    20701 ttatggcttg atggtacgca tgatcgggtt ttgcaggacg atcaatgtct cggtggactg
    20761 aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcgt cgatcgaacg
    20821 gcacattact tttataaaga tgctgtagtg gcctgttgtg taataggctt cagtgacttc
    20881 agcaaggctt tccagctttg ccagcgcgga agggtagtct ttggcgctct ttaatataat
    20941 gccgataaag cagcctacgt cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc
    21001 ggtaatgatc cccgcctgct tcattttctc tactcgaacg tgaatcgtcc ccggactgac
    21061 gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag
    21121 gatgccacgg tccagattgt cgatcagata attttccata ggattttctt atgcagattg
    21181 atgattcatt ctattttagc cttctttttt aatgaatcaa aagtgtgtta ggctttttat
    21241 tgaatgatta ttgcatgttt gtcggttttt gttgcttaat cataagcaac aggacgcagg
    21301 agtataaaaa atgaaaaccg cttacattgc caaacaacgc caaattagct tcgtgaaatc
    21361 tcacttttct cgccaactgg aagaacgtct ggggctgatc gaagtccagg cgccgattct
    21421 tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt
    21481 aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg cgaagtggaa
    21541 acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa
    21601 agcccttcgc cccgatgaag accgtctttc tccgttgcac tcggtctatg ttgaccagtg
    21661 ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc tcgactctga aaagcacggt
    21721 agaggcgatc tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag agtttggcct
    21781 ggcaccgttc ctgccggatc agatccactt cgtacacagc caggagttac tgtctcgtta
    21841 tccggatctc gatgccaaag ggcgtgagcg ggcgatagcg aaagatcttg gcgcggtatt
    21901 ccttgtcggg attggcggca agctgagcga tggtcatcgc cacgacgtgc gcgcaccgga
    21961 ttatgatgac tggagcaccc cgtcagagct gggtcatgcg ggtctgaacg gcgatattct
    22021 ggtgtggaac cctgtactgg aagatgcgtt tgagctttct tccatgggga tccgcgtgga
    22081 tgccgacacg ttgaagcatc agttggcgct gaccggtgat gaagatcgtc tgcagctgga
    22141 atggcatcag gcgctgctgc gcggtgaaat gccgcagacc atcggcggcg gtatcggcca
    22201 gtctcgtttg accatgctgc tgctgcaact gccgcatatc ggccaggttc agtgtggtgt
    22261 atggccagct gctgttcgcg agaacgtccc ttctctgctg taataattta tcgccgccag
    22321 cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc gaaaatgcgc
    22381 atgatgccgg gtttgccgtg tgccgacatc gccacggcat gaaagcgatg ctgatgtacc
    22441 cgctgcagct ctttcacttt actcgtcacg tcgtcaggca accgctgagc gataaaatca
    22501 gaaatcacca ccgcatcggc atcaaaccat tccctgcttt gcaagcgttc cataatggcg
    22561 cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa acggattgct
    22621 tgttcgatgc cttgggggcc tgaaagctca taacggacga tctcggtgga aaatagcata
    22681 atatagcagc gccggttttc agcgagagca atgcgcatca aggccaggca gaacgctttc
    22741 gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacacagac aataaacggc
    22801 ccgcgcggct gttcatcgta atctttatgt accaccggac gttcgatcac tttttcacgc
    22861 cacgactcac cgtgcaggcg ataggtgagc aactgttttt ccaccagccg acggtaaaac
    22921 tcatactcca gttccgttat ccctagtgtc gccagttctg gcggcagaag acgtaaaata
    22981 tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg
    23041 gtgcggaagg tttccatctg cgcatcgttg cgcggtattg atttggcttc ccgagaacgc
    23101 cccagctgct ctgccaggcg tttcagttcc ggctgttcgt taagaaattc accgtatttc
    23161 acaatcaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca cagacgacca
    23221 gctgcagtat tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt catgcgttct
    23281 tgaacttcac tcaacagttg ttcgcgttct tcttctaata gctgttgatt taacgtcgtt
    23341 gcttgcacga tcagacttaa acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtt
    23401 attgtgctgt tagcatcaac caactgccgg gcttgttctg cccatgggga attcagacga
    23461 tgcagtaagt ccaggatctg tggtagctgc acaataaact gcggcgtgga gaggagctgg
    23521 ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc atctttcagc
    23581 cgactgcgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc cttcaggcgt
    23641 gggaattttt caaagaagac cgccagctgc ggtgaggcca gcagtgcgat gatcatctct
    23701 tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag cgttagcatt
    23761 gttgtgcctg gtgaatctgt gcaccgacat cctgtaggct ggcttcaatg cgacctaacc
    23821 agtcgccagg gataaacaga catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc
    23881 agtcgctttc aagctcttcc agttgttgtt ttatttcacc aggcagacct tcagccgatg
    23941 aaccgggaag tgccagcgta ctgccttgta aacttacatc gcgaacgaca agatgttggg
    24001 cgctatcaac ttccagattc agtttctggg caaagccgat accgttcagt ttaccgcgaa
    24061 tttcaccacc tttgctcagc cactgttcca gcgcgctacg ctcaaaggag atatgaacca
    24121 cttccatatc atgcagtttt aacggttttt gcagcagtag agtcagagtg gaagcagtaa
    24181 cgttaacagg aagttgatac tgctgacgac ggctgaaaat gccgcccaga cgaattaccg
    24241 ttaaggctgt tttatcgctt tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc
    24301 caaggcgagt caacatccct tgctgttgcc aggcatgacc ggtcatcaat acatcaattt
    24361 gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc agcaaaatga
    24421 gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc gctggcctgc aataatcgga
    24481 tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta tccagttgca
    24541 ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca ccaatctctt
    24601 tctgccagcg ttcatattct tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt
    24661 tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcctgc actttatcta
    24721 accacagacg aatcagcatg cggtcatata acgcttccag actgctgtct gcttccggca
    24781 gctcgttgga ggccgccacc agcagacgca tcgggatttt ttctacgtgt gcaccgttgc
    24841 ggaactggcg ctcgttaatg gcggtgagca aggtattaag aattgccggg cccgctttcc
    24901 agatctcatc cagaaagaca atttctgctt ccggcaggta accgctggtt aaacgttcat
    24961 agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacttct tccggcgtgg
    25021 agaagcgggt catcagatat tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc
    25081 gggcgatcaa acttttggca atacctggcg ggccaaggag gaacacactt tcaccactta
    25141 atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc ttttccaacg
    25201 aactgctcag gcgggaaatt ctttccgcta ataaatgagg gtgagccata atgaagtggc
    25261 gtcctttcgt caaaagttct gcgtaaattg cgagtataga cgtttcttgc tggtggctaa
    25321 aatagtctca aag
//
LOCUS       DAJVXP010000053        24999 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVXP010000053 DAJVXP010000000
VERSION     DAJVXP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.053"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            196..384
                     /locus_tag="OQS10_002049"
     CDS             196..384
                     /locus_tag="OQS10_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS2293078.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="OQS10_002050"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="OQS10_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS2293079.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="OQS10_002051"
     CDS             1145..1309
                     /locus_tag="OQS10_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS2293080.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1502..3763
                     /gene="bcsA"
                     /locus_tag="OQS10_002052"
     CDS             1502..3763
                     /gene="bcsA"
                     /locus_tag="OQS10_002052"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS2293081.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="OQS10_002053"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="OQS10_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS2293082.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="OQS10_002054"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="OQS10_002054"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS2293083.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="OQS10_002055"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="OQS10_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="OQS10_002056"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="OQS10_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS2293084.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="OQS10_002057"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="OQS10_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS2293085.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="OQS10_002058"
     CDS             14459..15955
                     /locus_tag="OQS10_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS2293086.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="OQS10_002059"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="OQS10_002059"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS2293087.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="OQS10_002060"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="OQS10_002060"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS2293088.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="OQS10_002061"
     CDS             18049..20109
                     /locus_tag="OQS10_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS2293089.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="OQS10_002062"
     CDS             complement(20291..21613)
                     /locus_tag="OQS10_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS2293090.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="OQS10_002063"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="OQS10_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS2293091.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="OQS10_002064"
     CDS             complement(23086..23985)
                     /locus_tag="OQS10_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2293092.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>24999
                     /locus_tag="OQS10_002065"
     CDS             24505..>24999
                     /locus_tag="OQS10_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS2293093.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRA"
BASE COUNT         5864 a         6338 c         6966 g         5831 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tgaggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgtgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcacc atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgctg gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggtt caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc gacgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcg
//
LOCUS       DAJVXP010000054        24837 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVXP010000054 DAJVXP010000000
VERSION     DAJVXP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.054"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(29..829)
                     /locus_tag="OQS10_002066"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="OQS10_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="OQS10_002067"
     CDS             1396..1761
                     /locus_tag="OQS10_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2293094.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="OQS10_002068"
     CDS             1801..2457
                     /locus_tag="OQS10_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS2293095.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="OQS10_002069"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="OQS10_002069"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS2293096.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="OQS10_002070"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="OQS10_002070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS2293097.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OQS10_002071"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OQS10_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="OQS10_002072"
     CDS             5476..6393
                     /locus_tag="OQS10_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS2293098.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="OQS10_002073"
     CDS             6472..6654
                     /locus_tag="OQS10_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS2293099.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="OQS10_002074"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="OQS10_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCS2293100.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="OQS10_002075"
     CDS             complement(8516..9331)
                     /locus_tag="OQS10_002075"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCS2293101.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OQS10_002076"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OQS10_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS2293102.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="OQS10_002077"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="OQS10_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS2293103.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OQS10_002078"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OQS10_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS2293104.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OQS10_002079"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OQS10_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS2293105.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OQS10_002080"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OQS10_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS2293106.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OQS10_002081"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OQS10_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS2293107.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OQS10_002082"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OQS10_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS2293108.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OQS10_002083"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OQS10_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS2293109.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OQS10_002084"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OQS10_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS2293110.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OQS10_002085"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OQS10_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS2293111.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OQS10_002086"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OQS10_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS2293112.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OQS10_002087"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OQS10_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS2293113.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OQS10_002088"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OQS10_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS2293114.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OQS10_002089"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OQS10_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS2293115.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OQS10_002090"
     CDS             complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OQS10_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS2293116.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OQS10_002091"
     CDS             complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OQS10_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS2293117.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21825..22139
                     /gene="fliE"
                     /locus_tag="OQS10_002092"
     CDS             21825..22139
                     /gene="fliE"
                     /locus_tag="OQS10_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS2293118.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22473..22805
                     /gene="emrE"
                     /locus_tag="OQS10_002093"
     CDS             22473..22805
                     /gene="emrE"
                     /locus_tag="OQS10_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS2293119.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22974..23525
                     /gene="ybcL"
                     /locus_tag="OQS10_002094"
     CDS             22974..23525
                     /gene="ybcL"
                     /locus_tag="OQS10_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS2293120.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23535..24332
                     /locus_tag="OQS10_002095"
     CDS             23535..24332
                     /locus_tag="OQS10_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2293121.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6112 a         6136 c         6351 g         6238 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg tgcggtacgc tggtttactt
     7501 cgcaaccgga acacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataatttttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gagattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    19201 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac
    19261 atctcgtcga taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg
    19321 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    19381 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc
    19441 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    19501 agacgttcat cgaacaacgc cagaatatcg gcggcctgcg cgcgcttcag atgcaccaga
    19561 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcact ctgcggctcc
    19621 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    19681 aggctggcgg cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    19741 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatag
    19801 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcact
    19861 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    19921 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19981 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    20041 gacttctgcc cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag
    20101 gcggacttcc accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt
    20161 catcgcctcg gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc
    20221 caccagcagt accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg
    20281 ttgccagaac ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac
    20341 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgct tcgcgggtca gatcttcaat
    20401 ttgcttcatc tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt
    20461 caccactacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    20521 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    20581 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    20641 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    20701 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc
    20761 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt
    20821 gacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    20881 ctgaatacgg ccttcgacat cgctggcata tttcaactga gcgtcattaa gatcgcgccc
    20941 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    21001 cggcagacca gcaacggcgc tggaaaccag gtgcacaatg gcgctaattt gcccttcatc
    21061 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    21121 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    21181 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    21241 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    21301 gggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    21361 aatagcgccg ctggcttcgc tgaagcagta aggaatattc atttgcgtca gttggctgac
    21421 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    21481 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    21541 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    21601 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    21661 actcactccc gggtcagcaa actcgaaaaa ttgacgctgc cattatttgt gctttcgttt
    21721 ttttctatcc gccaataaac ccgttttttt tgttgctatt tagcgccttt gtcttattga
    21781 cttactggta agctttgcta ccagaaatta tccgggagat gaaaatgtca gcgatacagg
    21841 ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt gcgcaggaat
    21901 cactgccaca accgaccatt agttttgccg ggcagctgca cgccgcgctc gatcgcataa
    21961 gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt gaacccggcg
    22021 tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg caaatgggga
    22081 ttcaggtgcg taataagctg gtggcggctt atcaggaagt gatgtccatg caggtgtagg
    22141 ttttgtaacc tgttgttaat tacatccgat ctcaccgcgt ggggcatgga tggggcaaac
    22201 tcactcaatt tctggtttaa aatggatacc tgatcctgaa agttttctgc catccacgat
    22261 ccgtaaacgc gataacatgt tttgtcttcg ctttgctcat aggaagagtg aactcttttg
    22321 ttagcctggg gttccttctg atcattattg ttcctgcctt tatctcttgc cacgccagag
    22381 ccccctggat acacatacac caggatgaaa acggtgagtt gtgttcaaac tgtagtacaa
    22441 ttctctccag tatgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggcg
    22501 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagagggg tttacacggt
    22561 tatggccctc tgttggcaca attatttgct attgtgcatc attttggtta ttagctcaga
    22621 cactggctta tatacctaca gggattgctt atgctatctg gtcaggagtc ggtatcgttc
    22681 tgattagttt gctttcctgg ggattttttg gccaacgact ggacctgcca gccatcatag
    22741 gcatgatgtt gatttgtgcc ggtgtgttag tcattaactt gttgtcacga agcgcaccgc
    22801 attaaaaaat aatcatcctt gagataacag gaatctgatt tgtgcatatt atggctaatg
    22861 ttcggggcaa ctttataatt atgattgcat ttgcgcgatc attgcgcctt tttgcataat
    22921 gagatcaatg cacatcaaat aaaataacga taggtcacaa caaagaggtt tttatgaaaa
    22981 aacttatcgt ttcaagtgta ttggcattca taacattttc tgcgcaggcc gcagcatttc
    23041 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtattttctg
    23101 gagttgggtg tgaggggggt aatacatcgc cctcattaac ctggtctggt gctccagaag
    23161 gcaccaaaag ctttgccgta actgtgtatg atccagatgc gcctacaggc agcggatggt
    23221 ggcactggac tgttgctaat attccagcaa cggtaacata tttgcccgct gatgcaggaa
    23281 gacgtgatgg tacaaaactt ccgactggtg ctgtccaagg ccgaaatgat tttggctatg
    23341 cgggatttgg tggagcatgt cctcccaaag gagataaacc ccatcattac cagtttaaaa
    23401 tatgggctct aaaaactgat aagattcctg tggattctaa ttccagcgga gcgttagttg
    23461 gttatatgct taatgccaat aaaattgcaa ccgctgagat aatacccgtt tatgaggtaa
    23521 agtaggataa gagtatgctg gcaaaagata ggactaacct aaagattgaa gaaatacgga
    23581 tgcataaaca tcatgagatt cacagggtta agcctcttat gccagcttca tgtcgtatcc
    23641 gtcagggaaa aaaagttatc aattgggaga cgcatagtct aactgttgat aataatcaga
    23701 taatattatt tccctgtgga tatgaatttt atattgcgaa ttacccagaa gcaggtcttt
    23761 atcttgcaga aatgctttat tatcccattg atctaattga aaagttccaa aatctttatg
    23821 cgataaccga tcaaattcgt aacacgacag gtttctgctt acctcagaat tccgagttaa
    23881 tatattgttg ggagcaacta aaaacatcta tttcccgagg cttctcaacg caaattcagg
    23941 aacacttagc aatgggcgtt ctactttcat taggagcaca tcatgttaat tgtttacttt
    24001 tatcagatag taaacaatca ttaataagtc gttgttataa cctaatgcta tccgaacctg
    24061 gaacaaaatg gacagcaaac aaggtagcga gatatctcta catttctgtt tccacattgc
    24121 atcgccgtct ggcaagcgag ggagtaagtt ttcaaagtat attggacgac gtgaggttaa
    24181 ataatgcgtt gtctgctata caaacgacag taaaacccat cagcgagatt gccagggaaa
    24241 atggttacaa gtgtccttct cgttttactg aaaggttcca taatcgtttt aagataacac
    24301 caagagagct aagaaaagcg tccagagagt aaaagtattt tatgaaggag caacagcatc
    24361 gatttttatt ttggaaaata aatacggcat actcaaccta ttggagcctg aacaatccgg
    24421 tgacttctgc gctaatcggg gacgtttata agcacatata attgatacta tcttcctcca
    24481 tcacaatttc agaaatgcac gtgtgatttt tttgcatccg gcgtttggac tatgtggagt
    24541 tcaagagtgg gaaagttaca agaggttgag gggataatta aagggatgct tatgacatta
    24601 gatatccgga taggtacgga tatcgttgtt attaaatcta gttcgttatt attaaatcta
    24661 gtttatacgt taagatgcag ctacagcaga aattgccact gttaattttt tcatcgtgag
    24721 cccttttatt tgaactatta tttaaaaatg atgtcactgc actacaaata ttcatccaat
    24781 caatgagatt atttcaagat gtaagtttta gtttctcatt taaattgtga agtagat
//
LOCUS       DAJVXP010000055        23952 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVXP010000055 DAJVXP010000000
VERSION     DAJVXP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.055"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(39..401)
                     /locus_tag="OQS10_002096"
     CDS             complement(39..401)
                     /locus_tag="OQS10_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS2293122.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="OQS10_002097"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="OQS10_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS2293123.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="OQS10_002098"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="OQS10_002098"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS2293124.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="OQS10_002099"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="OQS10_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS2293125.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /locus_tag="OQS10_002100"
     CDS             complement(4828..5037)
                     /locus_tag="OQS10_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293126.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="OQS10_002101"
     CDS             5322..5684
                     /locus_tag="OQS10_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS2293127.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6227..6905
                     /locus_tag="OQS10_002102"
                     /pseudo
     CDS             6227..6905
                     /locus_tag="OQS10_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6949..11799
                     /locus_tag="OQS10_002103"
     CDS             6949..11799
                     /locus_tag="OQS10_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCS2293128.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12167..13822
                     /gene="lldP"
                     /locus_tag="OQS10_002104"
     CDS             12167..13822
                     /gene="lldP"
                     /locus_tag="OQS10_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS2293129.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13822..14598
                     /gene="lldR"
                     /locus_tag="OQS10_002105"
     CDS             13822..14598
                     /gene="lldR"
                     /locus_tag="OQS10_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS2293130.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14595..15785
                     /gene="lldD"
                     /locus_tag="OQS10_002106"
     CDS             14595..15785
                     /gene="lldD"
                     /locus_tag="OQS10_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS2293131.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15981..16454
                     /gene="trmL"
                     /locus_tag="OQS10_002107"
     CDS             15981..16454
                     /gene="trmL"
                     /locus_tag="OQS10_002107"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS2293132.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OQS10_002108"
     CDS             complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OQS10_002108"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS2293133.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OQS10_002109"
     CDS             complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OQS10_002109"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS2293134.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OQS10_002110"
     CDS             complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OQS10_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS2293135.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OQS10_002111"
     CDS             complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OQS10_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS2293136.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELLIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19350..19781)
                     /locus_tag="OQS10_002112"
     CDS             complement(19350..19781)
                     /locus_tag="OQS10_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS2293137.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20026..21570
                     /gene="gpmM"
                     /locus_tag="OQS10_002113"
                     /gene_synonym="pgmI"
     CDS             20026..21570
                     /gene="gpmM"
                     /locus_tag="OQS10_002113"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS2293138.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21580..22863
                     /gene="envC"
                     /locus_tag="OQS10_002114"
     CDS             21580..22863
                     /gene="envC"
                     /locus_tag="OQS10_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS2293139.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22867..23826
                     /locus_tag="OQS10_002115"
     CDS             22867..23826
                     /locus_tag="OQS10_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS2293140.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6047 a         6108 c         6211 g         5586 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 agtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgaga
     5821 ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata tatagaagat
     5881 atcatttatt aattaacaag cagttacgtc tctttttttc ggatttaatt gtgaaatttt
     5941 aatcatcata ttcatacaat aaattatctg taaatttttt cccagacatg gtttctttca
     6001 tatttgacat ttattaataa tcatcattag aatccacgaa aataatatat tcatacaaac
     6061 acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa
     6121 tcgacacaca tcactgtcat taatgtaaag caacatagat caccaataat acaatggaca
     6181 actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa
     6241 gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat
     6301 tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacatt aaaattgatc
     6361 tacttgaaaa acaacaagca cagctaaaga gtcaactgga aactattcaa aaacaacaaa
     6421 ctggcataat aagcagtaca aagactttaa ctcacgtaat taaatccgtt aaagatcaac
     6481 aaaacacttt tatttttacc gagtttaacc cagcaaaaac gaagtatttc atcctgaaca
     6541 atggttcggt ggctttagcg ggtcgagtgt tatccattga cgccacagag aatggcagtg
     6601 ttattcatat ttcactggtc aacttattaa gtacacctat ctcaaatatt ggttttaatg
     6661 cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg caacaattac
     6721 ttttcaacac atcaatgaaa tccacattga aattattacc aggtcaatgg caagacatta
     6781 atttgaccct gaagggtgta tcgcccaata acctgggata tctgaagtta gccatcaaca
     6841 tggaaaatat tcagttcgac aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa
     6901 aataaatgca gttagtacgc ttataacaag gaatagcgag catcattaat gaacaaaata
     6961 tttaaagtta tctggaaccc tgcgacaggg aattatactg ttaccagcga aacggcaaaa
     7021 agccgtggca agaaatctgg gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga
     7081 atgttgtcgt cgtttggggc attggcgaat gccgggaatg acaacggcca gggtgttgat
     7141 tacggtagtg gatcagctgg cgacagctgg gttgctatag gcaaaggggc gaaagcaaat
     7201 acttttatga acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc
     7261 caatatagct ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt
     7321 ggggttagtg caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca
     7381 ttgggccaat actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt
     7441 gctatggggg actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact
     7501 aaagctactg agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat
     7561 tcaatggcgc tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt
     7621 agcagtgtag ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca
     7681 gcgaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     7741 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     7801 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     7861 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     7921 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     7981 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     8041 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     8101 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     8161 ctttatgcca ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt
     8221 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
     8281 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
     8341 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
     8401 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
     8461 gacgatgtcg aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat
     8521 acagcaagta ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac
     8581 cttcaggctg atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc
     8641 caggatacca ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact
     8701 gatgctgtta acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc
     8761 aatatcgcca ataacacttc caatattgcc actaacacca ccaacatctc taatctgact
     8821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     8881 acggcagctc atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg
     8941 acgactggca gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg
     9001 gcgacgaata ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg
     9061 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca
     9121 gctcatggca acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca
     9181 accagttccg atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc
     9241 tacttcggcg gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa
     9301 atcggtgaaa caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt
     9361 agcacgtctc tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa
     9421 cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac
     9481 tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat
     9541 gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc
     9601 attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt
     9661 gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac
     9721 ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc
     9781 agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg
     9841 ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt
     9901 gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg
     9961 ctgtgggatg agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa
    10021 gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg
    10081 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat
    10141 cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac
    10201 gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct
    10261 acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac
    10321 agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg
    10381 attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac
    10441 accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa ataccgccaa
    10501 atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg ccagttgcag
    10561 aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa ctcaacggca
    10621 gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat
    10681 ggtaatgcag gtattggtat cggtttgaac actctggttc tagctgatgc gatcaacggc
    10741 attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt
    10801 tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag
    10861 aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc
    10921 gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt cactgatgag
    10981 cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac taatctggat
    11041 actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg tagcaccaag
    11101 tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga tagcgttgct
    11161 attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac cggttccgtt
    11221 gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg tatcaccaac
    11281 gtagctgccg gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa
    11341 gcaggcggcg tgcgctacga caccaaagct gatggttcta tcgactatag caatatcacc
    11401 ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
    11461 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
    11521 gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
    11581 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc gggtgccagc
    11641 atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg
    11701 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
    11761 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
    11821 ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgcccc
    11881 gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct
    11941 ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
    12001 ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca
    12061 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
    12121 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
    12181 acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat
    12241 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
    12301 gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
    12361 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
    12421 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
    12481 gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt
    12541 cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
    12601 gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac
    12661 cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg
    12721 catcgacagc tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat
    12781 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc
    12841 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
    12901 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
    12961 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcaaagat
    13021 tgatatgacg ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
    13081 gttcctgttc ctgacagcta ccgtaacact gtggagcatc ccgccgttta aagccctgtt
    13141 cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
    13201 actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
    13261 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
    13321 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
    13381 ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc
    13441 cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt
    13501 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
    13561 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
    13621 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
    13681 tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt
    13741 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
    13801 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
    13861 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct
    13921 gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactgcgcga ggcgctggca
    13981 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
    14041 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
    14101 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
    14161 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
    14221 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
    14281 attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc
    14341 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
    14401 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
    14461 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
    14521 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
    14581 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
    14641 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
    14701 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
    14761 ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
    14821 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
    14881 cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag
    14941 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
    15001 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
    15061 tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa
    15121 atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg
    15181 gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc aaaccgaccg
    15241 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag
    15301 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
    15361 cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt tctaaccacg
    15421 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
    15481 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
    15541 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc
    15601 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga
    15661 aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg
    15721 tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg
    15781 catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg accgttcggc
    15841 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
    15901 cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct gctatcctgc
    15961 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
    16021 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
    16081 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
    16141 ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa aatccccagc
    16201 gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
    16261 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
    16321 tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaatctgt
    16381 ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat ccgggagcgt
    16441 tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac
    16501 aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc
    16561 catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc
    16621 ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
    16681 gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg
    16741 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
    16801 aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc
    16861 gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga
    16921 aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc
    16981 gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt
    17041 atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
    17101 ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat
    17161 tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt
    17221 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    17281 ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    17341 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc
    17401 cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg
    17461 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    17521 ctacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    17581 cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    17641 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc agccccgcca
    17701 tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atttcagcca
    17761 gcccacgggt gatcagtgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
    17821 ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg atgaaatccg
    17881 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga tcatcggcaa
    17941 aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc agttctttcg
    18001 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    18061 cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg
    18121 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    18181 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    18241 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    18301 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    18361 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    18421 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    18481 catgaacagc gcatcgaagt taatcggcgc aaggttcagt tgcgggaatg taccgcggga
    18541 taccacgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    18601 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    18661 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    18721 cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc
    18781 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt
    18841 ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag
    18901 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
    18961 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca
    19021 atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc
    19081 tcttcacgct tggcggcatt gccatcgatc agcaactcct gaaaactcac gcccttgctg
    19141 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    19201 ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag ggcggctgca
    19261 aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac
    19321 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg caagttttcg
    19381 ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc agctttcgtc
    19441 agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg
    19501 tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg caacagattg
    19561 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    19621 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    19681 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    19741 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    19801 cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag
    19861 cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc
    19921 ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt
    19981 agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa
    20041 aaacctatgg tactggtgat tctggatgga tatggctatc gtgaagaaca gcaggataac
    20101 gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg tccgcatacc
    20161 ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa
    20221 gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac
    20281 gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa
    20341 gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg cggcgtacac
    20401 agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa
    20461 atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg
    20521 ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt
    20581 ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac
    20641 ctgctgactc tggcgcaggg tgagtttcag gccgatactg ccgttgctgg tttgcaggca
    20701 gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt
    20761 cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac
    20821 cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag
    20881 aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg
    20941 gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac
    21001 aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac
    21061 ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg
    21121 gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg
    21181 gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta
    21241 ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg
    21301 gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac
    21361 ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac
    21421 ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc aggcggcaaa
    21481 ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg
    21541 actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat
    21601 accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc
    21661 gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa
    21721 ctcaaatcta ttcaggccga tatcgccaca aaagagcgcg cggtacgcca aaagcaacaa
    21781 caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc
    21841 cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg
    21901 aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgca
    21961 caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat tctcagcggt
    22021 gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga
    22081 caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa
    22141 ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg
    22201 aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga atcttccatc
    22261 cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct gcgtaacagt
    22321 attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg tgaggctcag
    22381 gcagttcgcg acaggcagaa agaagcgacg cgcaaaggca ccacctacaa accgaccgaa
    22441 agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc
    22501 tggccagttc gtggccctac gctgcatcgc tatggcgagc agctacaggg tgaattacgc
    22561 tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat tgccgacggt
    22621 cgggtgattc tggctgactg gctgcaaggc tacggtctgg tggtggtggt tgagcacggt
    22681 aaaggcgata tgagtcttta cggctataat caaagcgcgc tggtgagcgt tggttcgcag
    22741 gttcgtgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg cagaccttca
    22801 ctctatttcg aaattcgccg ccagggtcag gccgtcaatc cacagccgtg gttgggaaga
    22861 taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc
    22921 tcccctgtaa ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgaac
    22981 aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt acccgattca
    23041 ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt gttgattcat
    23101 ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctgcg cccggagatg
    23161 agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg
    23221 gggatcaata accacatggg cagcaagatg acctctaacc tgtttggtat gcagaaagtg
    23281 atgcaggcgc tggagcgtta taatctttac ttcctcgaca gcgtaaccat tggtaatacc
    23341 caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa ggtgttcctc
    23401 gacgattcac agaacgaagc ggacatccgt gtgcaattca atcgcgcaat tgacctagcg
    23461 cgtcgcaacg gatcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgttg
    23521 caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg
    23581 aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga cacaccgcgt
    23641 aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct
    23701 aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc
    23761 cagcatcagt ggcaaggctg gggcaaacag cctgaagcgg cgaagcttaa cgctagcgta
    23821 aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata
    23881 agcggaaaga ctacagtaac gcctgataac ccacctttac gctgcgcaca ttggtaatca
    23941 ggcgcttata ca
//
LOCUS       DAJVXP010000056        23399 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVXP010000056 DAJVXP010000000
VERSION     DAJVXP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.056"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..157)
                     /gene="cas6e"
                     /locus_tag="OQS10_002116"
     CDS             complement(<1..157)
                     /gene="cas6e"
                     /locus_tag="OQS10_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS2293141.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="OQS10_002117"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="OQS10_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS2293142.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="OQS10_002118"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="OQS10_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCS2293143.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="OQS10_002119"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="OQS10_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCS2293144.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4057)
                     /gene="casA"
                     /locus_tag="OQS10_002120"
                     /pseudo
     CDS             complement(2496..4057)
                     /gene="casA"
                     /locus_tag="OQS10_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4155..6854)
                     /locus_tag="OQS10_002121"
     CDS             complement(4155..6854)
                     /locus_tag="OQS10_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCS2293145.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7049..7201)
                     /locus_tag="OQS10_002122"
     CDS             complement(7049..7201)
                     /locus_tag="OQS10_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS2293146.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="OQS10_002123"
     CDS             complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="OQS10_002123"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS2293147.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="OQS10_002124"
     CDS             complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="OQS10_002124"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS2293148.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="OQS10_002125"
     CDS             complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="OQS10_002125"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS2293149.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12105..12467
                     /gene="queD"
                     /locus_tag="OQS10_002126"
     CDS             12105..12467
                     /gene="queD"
                     /locus_tag="OQS10_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS2293150.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12545..13816
                     /locus_tag="OQS10_002127"
     CDS             12545..13816
                     /locus_tag="OQS10_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2293151.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13807..14067
                     /locus_tag="OQS10_002128"
     CDS             13807..14067
                     /locus_tag="OQS10_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS2293152.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14084..14659
                     /locus_tag="OQS10_002129"
     CDS             14084..14659
                     /locus_tag="OQS10_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS2293153.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14807..15667)
                     /locus_tag="OQS10_002130"
     CDS             complement(14807..15667)
                     /locus_tag="OQS10_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS2293154.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15664..16443)
                     /locus_tag="OQS10_002131"
     CDS             complement(15664..16443)
                     /locus_tag="OQS10_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS2293155.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16421..17830)
                     /locus_tag="OQS10_002132"
     CDS             complement(16421..17830)
                     /locus_tag="OQS10_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2293156.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(17852..19306)
                     /locus_tag="OQS10_002133"
     CDS             complement(17852..19306)
                     /locus_tag="OQS10_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS2293157.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(19376..20192)
                     /locus_tag="OQS10_002134"
                     /pseudo
     CDS             complement(19376..20192)
                     /locus_tag="OQS10_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            20510..21787
                     /locus_tag="OQS10_002135"
     CDS             20510..21787
                     /locus_tag="OQS10_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2293158.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            21814..23299
                     /locus_tag="OQS10_002136"
                     /pseudo
     CDS             21814..23299
                     /locus_tag="OQS10_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5608 a         6209 c         5777 g         5805 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt ttattgagca gcagcgggaa atgatgttcg taccaacagc
     2941 gcgctttcat gttgtcgaaa tcatagccaa atccccacag gcccactttt gcctgcttga
     3001 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgact
     3061 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttga
     3121 ccgagtaaaa ctcgccgccc tctttaagta gtacacggta aggcgttaag ggatgctgcc
     3181 acatggcata gttagcgccg taatttttgg tcgtcatcag gctcagaagc gcgtcgctct
     3241 gctcaccgca aatatcgcag ttgccgactg tggtggtatt aaaatcaata cgaatacggc
     3301 gaggcattcc ccagtacgcc tggagtttat tgacctgctc atcggttacc accgcaccgg
     3361 ccagttcgct gatgcgcgtc gggccaagcc aggggaaaac cagatcgtca aattttttgg
     3421 gtagcggtaa atcggcttca tcctgcggca tgacattgat ccacagtttg cgccacaagg
     3481 gagtttgttg attaccctga tattcctgta attcaatcag agtggtcatc ggcccaccgc
     3541 cgcgtaaacc ggtgcggtag cctttgccac ctgacggcgc attcaactgt agggagaaca
     3601 gagctaacgc agagcaatgt gggcacaggt attccgttac accacgcttg ataaagtggt
     3661 ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa
     3721 ctttatctcc ggtgagcgcc tcgaaatcct gtataaatga aggggaatct ggcccaaact
     3781 ggaaagcatg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
     3841 cccagatatc atcccaacga cgatgatctt ttggcgcgaa gctggtttgt agtagcccca
     3901 gcaaaaactg ccataccgca ccctggagat ctgcccgcgg cgcagaaata tcgacaacat
     3961 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg
     4021 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
     4081 catcccaggt cggttgtcca tctttccatc cttttcggtg tatcaactat tcagagagtc
     4141 ctgtcgcaga catctcaacc aaccttgcca atcaatcctt ccgtagccga atacccacaa
     4201 ctttcgtcat cgttgactaa aataacgttt gccatctccg gatcttgtcg ctgttcaatg
     4261 caccacagcc tgaacgcctc tccctccagt aaagaaaact catcccgatg ttttttccac
     4321 cagcttcggc gcactctgac aacgctcatt tcccatgcgt gagcaccggt ggcataaggc
     4381 ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagactcctc agccagacgt
     4441 gtcgacagtt tttctgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
     4501 agcacaaact ttttagccat tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg
     4561 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agacggattc aataagaagc
     4621 cgggctgcgt gtggcatctg aatagcacct tgctcacgca gcacacgctg cgtcagccag
     4681 attcgcccat gatcgggata gacaaatgca ctgttacgca tggcactgcc aaaccattcg
     4741 tcgccaggag agtcgtccca gacgggagcc agaatcagca attcaggggg ggaacgctcg
     4801 tcctttccgt cacgctttaa ctgaccatta atatcgcgga tatgccgctg taatcgtcct
     4861 gctcgctgaa tcagcaaatc aacaggggcc aggtcggaga tcatttcgtc caggtcacaa
     4921 tcgacactct gctctaatac ctgagtacaa ataaggactt ttccggcacg ctgtgagcta
     4981 tcttctttac caaagcgtgc cagtgtctcc gtttcaattc gctggcgatc gctaaaagca
     5041 aagcggctat gaaaaagtga aaggctggaa gcaggaatga cgccgcgggc aagcagctga
     5101 cgatgaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca tttcccctga
     5161 cttaccgccg attcgatacg cgcaatacac tcttgttcac tatgaagcca acccacgctg
     5221 acgctacgct caacgtcttt gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg
     5281 acatgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg caggccctct
     5341 gtaccccgcg caaaggcagc gacgagtttg tcgcgctgct gttgagataa cgtggcagaa
     5401 agcaaaataa cgctgtttcc gccacgcgcc tgccgctcga tcaacccttc aagaatgcac
     5461 gacatgtaag cgtcacaggc gtggatctca tcagccagca ggattttgtt actcagcccc
     5521 agaagccgca gattattatg tttgaacggc atcaccgcca tcatcgcctg atccagcgtg
     5581 ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca ggccgcacat
     5641 ccctgactga acgtttgttc atccggttct tctgacccga ctaaatcacc agaccagagt
     5701 gattcattaa agcggtccat taatgtgcgg gcactatgtg ccagcaccag gctggggcgg
     5761 gattctggcg aatagaaagc aagccaggtt ttgaccagcc gatcgtacat ggcattggcc
     5821 gttgccattg ttggcagacc aaaaaacagc ccctgagcct tccccgcagc tatcaacctg
     5881 tgcgccagga taagcgccgc ttctgtttta cctgcgccag tcacatcttc cagaataaat
     5941 aactgcggtc cttgctggct gatatccaga tccagcacct tttgctgtaa cggtgtcgga
     6001 ttctcaataa aaggaaacag cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga
     6061 ggaaagacgg ttaaggcgtt ttgagcctga actaaagttt tctgccagta acctttaata
     6121 tccattgggt gtgcgacgcg tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc
     6181 acgacggttg cagagatata ccaggaaagg tgttttataa gttcaatgcc ctcatcatca
     6241 tcccagaatg cggggatctc tatgagcgga aacagtggtt tgatttcaag gagaaaatct
     6301 cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg atcagggggc
     6361 caaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct aagtttgcgc
     6421 ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg attccacaac
     6481 cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc ttcctgaatc
     6541 ttgagttcag ggggcaggta aagttgttga aaagagcggg caaattttcc aatatcgtgc
     6601 cagcacagca accaggcgaa aaattgagcc gcctgttcct tgtcagaaat ccctaattga
     6661 cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc catcgcggct
     6721 acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt
     6781 cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa
     6841 ggatatttac gcatatatta aaaattatct gtgatatata tcattcaata aaatatttac
     6901 attatgaata atcaaataat tttcatccgt aatatatatg aataataaac ataggcataa
     6961 tattcacata aataatataa attaaaaaag ggtgcaggag accaaaccat cacaattcag
     7021 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
     7081 taaactccat accacgttct ctgatactca actcacacaa cgagtcgcct accatcagcg
     7141 tgaatcccag tactgtaaaa cacagcacta tgattgccac aagggcatat tttgtcagca
     7201 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgctggc
     7261 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
     7321 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
     7381 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag
     7441 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
     7501 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
     7561 caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca
     7621 ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
     7681 gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag
     7741 caaaccaggt ttgcgcgtta agttctttca gagcccggtt catcggttcg actttgttga
     7801 tgtcattgta cttttcaatg ccttcaacgc cctgctccca cagttttccg tagcgtgctt
     7861 cctgccaggc tgcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg
     7921 ttaactcgtc aataaagcgg taggtttccg ggaacaagta acccgtatcg gtgaggatca
     7981 ccggaatatc cgggcgaatt tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     8041 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggct acgcggcctt
     8101 cagcgtccag tttttccagt tcggcgttag tttccgccag cgccagaata cgatctacct
     8161 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     8221 tgcctgatgc gacgctcacg cgttcttatc aggcctacaa gaccgggctg atggttaatc
     8281 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
     8341 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
     8401 cgccaggatt tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
     8461 gcggttgccg ccaagatgca ggttgtagcg acccggcgct ttacccacca ggcccacttc
     8521 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
     8581 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
     8641 acgctctgct tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt
     8701 ttcacgctgc ggcgtgacgg cattcattaa cccgctctct ttggcgatct tctcgatctt
     8761 cgctttctcg ctttccggta caccggtgat gatcagattc tggttcgccg taatgcggaa
     8821 atcgcctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata
     8881 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt
     8941 aacccagcca atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt
     9001 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
     9061 gtatttggtt ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac
     9121 gacggcttcg gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt
     9181 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt
     9241 gaagcctacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag
     9301 atcgatatcg ttctgcggcg ggatcactac cgtggttttg aacttacgcg gcaggtaggt
     9361 ctgaccaagg atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc
     9421 cgcatacgcg cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc
     9481 ttccgcgtgc agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
     9541 gttagctgtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
     9601 gttctttttc agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc
     9661 atagatggtg ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac
     9721 cccacccggc agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc
     9781 acgttcggca cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
     9841 gaagttgtcg cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc
     9901 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
     9961 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
    10021 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
    10081 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
    10141 atgtgggcac cgtcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct
    10201 tgtacataaa ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg cgtcagcacg
    10261 ccctctttga cgtagcgctg ccactccacc tggtagagga aatcttccgt aaagtgcggg
    10321 ttgccaaaga acagccagtt tttacccggc gcttcgtcgg cggcgcgttg ctgcataaag
    10381 gcacggaacg gcgcgatgcc ggtaccgggg ccaatcataa tcactggcgt ttgcgggtta
    10441 gcgggcaggc ggaaattgtc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc
    10501 acgcgatccg cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
    10561 cgcaccacac caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg
    10621 gaatacaggc gcggggtcag cgggcgcagc aggttaatca gcgcttcggc atccagttgc
    10681 gccggagaga aacgcaccat gtcgacaatc ggcgtggtcg cggcgtaatg ctgtaacttc
    10741 gctttatcgc ccaccagcgg cagcagcgtt tcgctgcggg taagcgtggc atagttctca
    10801 acaatgttgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc
    10861 aacgttttgc cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt
    10921 tctttcacca gtgccgggtc gttctgatac cagacaccca gcgcgtcacc cggctggtaa
    10981 cgcaggcctg aatcgcctaa gtcaatttcg atatgacgaa cgtctttttc agagttacgc
    11041 ccggtaattt tctggttaac cgaaaggctc gccaccagcg gcgcgtcttt gctgtacggg
    11101 ctggtgtgga tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc
    11161 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat
    11221 tcaacatcgg catcgacacg gtcgagcagg cgttcaccac ccagttccgc cagcttgctg
    11281 tcgaaatctt tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg
    11341 gcaaacgcgg tgttttccag ctttggcgct tttttggaga acaggaactt atgcagcgcg
    11401 acggcttctt ccggcggttc cccttcccct tgcgttgacg tcactacgat gagcagtttt
    11461 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
    11521 gctgctaata aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgag
    11581 gcggagataa tagttatacc cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca
    11641 gcaggctgct gattgagtac gccccagaaa tagccagaaa cccaggcaag ctgggtaggc
    11701 gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac
    11761 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
    11821 gcgccctgtc agcgcccata aaacagaaga gaagataagg ttaacggggt gaacggtgtg
    11881 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
    11941 ctttaacaac aaaatagatt aaccaaccca atgaaaaaca aatgaattta gccaatcatt
    12001 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt
    12061 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
    12121 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
    12181 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
    12241 cgcatacggg ctggattatc gatttcgctg aactgaaagc ggcgtttaag ccaacttatg
    12301 agcgcctcga tcaccattat ctcaatgata ttccaggtct ggaaaaccca accagcgaag
    12361 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
    12421 tggtaaaaga aacctgcacc gcagggtgta tctatcgcgg cgaatgataa gagtgtgtcg
    12481 gcggtcaatt tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca
    12541 tgacatggaa gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg
    12601 cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc
    12661 cggcagcaaa aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc
    12721 gcaatttcat ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt
    12781 aacgcccgat ggcgtaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
    12841 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
    12901 catccccgga gcgacggtgg atgcactgta tgaagaaaac ggcagagtct gtggcgttat
    12961 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
    13021 cctggctgaa cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
    13081 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
    13141 cgaaggcgca gcgttgctgt tcagcggcgg aatctgtgat gacttacccg gcggcgcatt
    13201 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac
    13261 gcaaagtcgt gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg
    13321 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggagc cagaaggtgg
    13381 cttgcacagt atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt
    13441 gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc
    13501 acaggcggcg gcacaaacgc taataagcgc ctgccagcac cgcgagccgc aaaatctgtt
    13561 tgcgctttat catcacaacg tagagcgcag cctgctatgg gatgttctac aacgttatca
    13621 gcatgttccg gcgcttttgc aacgccctgg ctggtatcgg gcgtggcctg cgttaatgca
    13681 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
    13741 attctggcgt catttacgtc gtcatggcct gtggcatctg gcgggcgatg ttatcaggag
    13801 tctgcgatgt ctgtagccca taatctctgg cgcgttgctg atgcgccgca cattgttccg
    13861 gctgactccg ttgagcgtca gatggtacaa cggttgatta acgcctgtcc ggcaggtctt
    13921 ttttcgctca caccggaagg tgacttacgc gttgattatc acggctgcct ggagtgtggc
    13981 acctgccgtt tgctgtgcga cgaatccaca ctacaacagt ggcgctatcc gccttccgga
    14041 ttcggcatca cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct
    14101 actccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggctat
    14161 cgattctgaa tgccagttta tttccgtgtt gtacggcaat atctgcacca tcagtaatat
    14221 cgttaaaaag attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg
    14281 cgcgtcaaat aaagaagtcg ttattcagtt tttgaaactg gtgaccgagg cggacggcat
    14341 tatcagtacc aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca
    14401 tcgcctgttt attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc
    14461 gaatccggat tgtattgaga tcctgccagg cggtatgccc aaagtgctgg gctgggtgac
    14521 agagaaaatc cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg
    14581 taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt
    14641 agcgaaaaaa ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg
    14701 ccgcacgcca catccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct
    14761 acaagtcccc aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg
    14821 cgtgaatgtt tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat
    14881 ctgcctgtga aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat
    14941 tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag
    15001 gtgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca
    15061 ctcgcgcccg actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct
    15121 ggttatctgc ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt
    15181 cgcgagtaac atttttcaga tcctctgtgc taaccagcca gtccggaagc gcacccggga
    15241 caatgttcag ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac
    15301 gcgccaggga aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc
    15361 cccagtggga tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc
    15421 ttgcaccatg taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg
    15481 gaaagagaac aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct
    15541 gttccgccac tacaggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat
    15601 cctgcgccgc cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga
    15661 tattcatggc tgcatccttt ggcgtaaata gtcctgccat agcttttgag ctttttctgc
    15721 taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag
    15781 acactgcatc gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc
    15841 agccattcgc tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc
    15901 aatcaccgca ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag
    15961 ggtgataaac agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag
    16021 catttccgcc agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt
    16081 aattatcaga tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc
    16141 cggcgcaaag cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc
    16201 catgagatag cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga
    16261 catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc
    16321 accgagtaaa cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg
    16381 ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt
    16441 cattcgcacc tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc
    16501 atagccaggt cacaacaaaa tcaaccaaca acactgtcgc caggagcaat aaggtgactt
    16561 gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc
    16621 gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg
    16681 caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa
    16741 gcagcagcgt taatgaactg ccggaaggca aacaggccat cactaccagc gttgccgcca
    16801 gcagcaaaaa acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc
    16861 ccagcagcgc gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt
    16921 ccagaccaat agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta
    16981 cgaggcagac aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca
    17041 aggttttgat gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac
    17101 caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc
    17161 gtggtgattc tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg
    17221 atgccagtag ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac
    17281 ttgccagcac atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat
    17341 ggcggcgcgg ggaaaattca gccagcaagg tgtgacctac tgaataatcg cctcccagac
    17401 caatgccaat caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgta
    17461 aaaacgaagc aagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat
    17521 ggtcggagat ccacccaaga accaggctgc caaggaacaa accaaggagc gccgagccgc
    17581 cgatcattcc cgccataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt
    17641 aaccaatgac gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa
    17701 tgcggcagtg aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct
    17761 ttaccccttc attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac
    17821 gccccgtcat cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg
    17881 tattcataat gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc
    17941 tgccgtgttc cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca
    18001 tcgaaccgcc gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact
    18061 tgtcgatttc ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt
    18121 tggtgccgtt ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt
    18181 gcgggaactc agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc
    18241 agcagccgga cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt
    18301 cggcagccac tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc
    18361 tgtccacgcg ctggcattgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg
    18421 ccttcgcaat gcgagggtta ccttcagcca taaagatcag cacgcatttt ccgtctgcaa
    18481 aatgggtgaa gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat
    18541 acccttccac catgacttca cgcaggatgt tgaagtcggt tttcatgtct tccaggatat
    18601 agccgtagaa gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga
    18661 tatagcaaag tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc
    18721 ggcgtggcac gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa
    18781 cgaccatatc ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta
    18841 ccaagccgcc catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt
    18901 tttcacgcaa cgcattttcc agcacctcca gcggaacgcc acattgcgcc gtcgcctgca
    18961 tattctcaat atcaatatta atgatttgat tcatggcgga gccgtcaagc accaccgagt
    19021 tttccacaac ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt
    19081 taattttgtg cgcattcata aaattcagca cacgggatac ttgctctgtg gaaccgagtt
    19141 ttacgaccgc tgccggaatc ggcaaagtat aaatgccatg aatatccgga aatttacgaa
    19201 aacggtcaat actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa
    19261 caatttcctt tagttggtcg acaatcgctg cgcgagataa agacatagta attccttcct
    19321 gataataaaa atggaataat cattaataag agaaaacaga aaccaccgtt atggattagc
    19381 gcactaaata accaccattg accactaata aatgcccatt gacataattt gaagccggac
    19441 ttgcgaggaa tacagctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa
    19501 tatgatcaag aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg
    19561 ttgcataata gccaggagca ataccattta cctgaatatt atattgacct agttcatcac
    19621 aataagcttt ggtgaatccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt
    19681 gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga
    19741 tcataatttt tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca
    19801 tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat
    19861 tgtttaccag aatatcaact gtaccgaaac gctcacagca agcagcgata atcttctgcg
    19921 gcgcgccttc tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa
    19981 tcatttcctt tgtttcgccg ttatctttca atcatttcct ttgtttcgcc gttatctttg
    20041 acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc aaatgcctgg
    20101 cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc
    20161 attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa
    20221 aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc tctggtaact
    20281 ggagataatt tttagcataa cggcataact gctaatgtga tcgtaatcac agtgtgatat
    20341 tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca acacctctgc
    20401 tttccatgtt atttcttatt gcaattacta gagacctgag aaaagtaatc cacctgaaag
    20461 ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata tgcaacacaa
    20521 ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg ttagttttga
    20581 cctggcttat ttacgttata tttttcaaat tcccatggcg aaatttatgg gattcagcaa
    20641 taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc tttatgcccc
    20701 cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg cgatgatcat
    20761 taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa tgctctgtat
    20821 tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc
    20881 ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg ggctggatgg aagggctgcg
    20941 cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc gctttgcacc
    21001 ggatgacagc gccagcctga aaacagtcat tatcatctac agtgtggttt acatcttgtt
    21061 ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg ccaataacga
    21121 agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg
    21181 gtattgcagc atggtgattt ttggtgtctt cacaatctac gccattctga gttactccac
    21241 caactatctg accgaaatgt acggcatgtc gctggtggca gcgagctaca tggggattgt
    21301 gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga ggcattatca ccacctacag
    21361 taaagtgaaa tcccctaccc gcgtgattca aatcctttcc atcatcggtt tgctggcgtt
    21421 gacagccctg ctcgtcacga actctaaccc ccaatcggtc gcgatgggga ttggcctgat
    21481 tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct gccccggcga
    21541 agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg tgattggatt
    21601 cctgccggat gtcttcgttt acccgattat cggccactgg caagacaccc tgcccgctgc
    21661 agaagcctac cgcaatatgt ggctgatggg catggcggcg ctcggcatgg tgattgtctt
    21721 tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa tagctagcag
    21781 caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat catagggatt
    21841 gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa tgtggtttgc
    21901 gaaggtaaag gcttattaca gccgatgcac acgcctgatg ccgatactgc agaacatcct
    21961 gacgacgatt tatgggcatc attatgtttt gcaggtcacg atttgatgag ccagtttgcc
    22021 gggaataaag aagatattgt tggcatcggc ctgggctcca tccgttgctg ccgtgcgtta
    22081 ttgaaagccg acggcacgcc agctgcgccg ttgattagct ggcaggatgc acgcgttaca
    22141 cgcccttacg aacatacgaa tcctgacgtg gcgtatgtaa cctctttttc gggttatctg
    22201 acgcatcgct taaccggcga atttaaagat aatatcgcca actattttgg tcagtggccg
    22261 gtggattata agacctgggc atggagcgaa gatgccgcag tcatggagaa gtttaatatc
    22321 ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg ttcttgggca tatcacacct
    22381 caagccgcgc tggcgacgca tttcccggca ggactgccgg ttgtttgtac caccagtgat
    22441 aaaccggtag aagctctggg agccggatta ctggatgatg aaactgcggt aatttctctt
    22501 ggcacttaca tcgcattgat gatgaacggc aaagccttgc cgaaagatcc ggtggcgtac
    22561 tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa
    22621 ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat tcaggatgcc
    22681 agggcgcagg atctttcacc ggaagattta ctcaacaaga aagcctctag tgtgccacct
    22741 ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga accgtacaaa
    22801 cgcgggatta tgatcggctt tgattccagc atggattacg catggatata tcgttcgata
    22861 ttggaaagcg tggcgctgac gctgaagaac aattacgaca atatgtgtaa tgaaatgaat
    22921 cactttgcga aacatgtaat cattactggc ggcggttcga acaggatctg tttatgcaga
    22981 tttttgccga cgtgttcaac cttccggcac gccgtaacgc cattaacggt tgtgcaagcc
    23041 tgggggcagc gattaataca ggggtaggtc tggggctata cccggattac gcaacggctg
    23101 tcgataaaat ggttcgcgtg aaagatatct ttataccgat tgagagcaat gccaaacgct
    23161 acgacgcgat gaataaaggc attttcaaag acctaaccaa acatactgat gtgatcctga
    23221 aaaaatcgta tgaagtgatg catggggaat tggggaattg gggaatgcgg attcgatcca
    23281 gagctggtcg aatgcgtgaa taaggtaggc cgggcggaaa actgcccggc ctgaacatac
    23341 ctgaatggtt atccccgctg gcgcggggaa cataagttcg acaacttaaa aaatgataa
//
LOCUS       DAJVXP010000057        23377 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVXP010000057 DAJVXP010000000
VERSION     DAJVXP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.057"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="OQS10_002137"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="OQS10_002137"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS2293159.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="OQS10_002138"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="OQS10_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS2293160.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OQS10_002139"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OQS10_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS2293161.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OQS10_002140"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OQS10_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS2293162.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OQS10_002141"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OQS10_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS2293163.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="OQS10_002142"
     CDS             5280..5738
                     /locus_tag="OQS10_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS2293164.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="OQS10_002143"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="OQS10_002143"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS2293165.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="OQS10_002144"
     tRNA            6790..6874
                     /locus_tag="OQS10_002144"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OQS10_002145"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OQS10_002145"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OQS10_002146"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OQS10_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS2293166.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OQS10_002147"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OQS10_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS2293167.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OQS10_002148"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OQS10_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS2293168.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OQS10_002149"
     CDS             complement(10156..13899)
                     /locus_tag="OQS10_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS2293169.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OQS10_002150"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OQS10_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS2293170.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OQS10_002151"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OQS10_002151"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS2293171.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OQS10_002152"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OQS10_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS2293172.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OQS10_002153"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OQS10_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS2293173.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OQS10_002154"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OQS10_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS2293174.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OQS10_002155"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OQS10_002155"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS2293175.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OQS10_002156"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OQS10_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS2293176.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OQS10_002157"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OQS10_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS2293177.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..>23377
                     /locus_tag="OQS10_002158"
     CDS             23294..>23377
                     /locus_tag="OQS10_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS2293178.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5860 a         6116 c         5626 g         5775 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cgaatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtc
//
LOCUS       DAJVXP010000058        23257 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVXP010000058 DAJVXP010000000
VERSION     DAJVXP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.058"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..455
                     /locus_tag="OQS10_002159"
     CDS             <1..455
                     /locus_tag="OQS10_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS2293179.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OQS10_002160"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OQS10_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS2293180.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OQS10_002161"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OQS10_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS2293181.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OQS10_002162"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OQS10_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS2293182.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OQS10_002163"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OQS10_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS2293183.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OQS10_002164"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OQS10_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS2293184.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OQS10_002165"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OQS10_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS2293185.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OQS10_002166"
     CDS             complement(6424..6918)
                     /locus_tag="OQS10_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS2293186.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OQS10_002167"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OQS10_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS2293187.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OQS10_002168"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OQS10_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS2293188.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OQS10_002169"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OQS10_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS2293189.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OQS10_002170"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OQS10_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS2293190.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OQS10_002171"
     CDS             complement(12082..12369)
                     /locus_tag="OQS10_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS2293191.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OQS10_002172"
     CDS             complement(12488..13375)
                     /locus_tag="OQS10_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS2293192.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OQS10_002173"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OQS10_002173"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS2293193.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OQS10_002174"
     CDS             complement(14612..15106)
                     /locus_tag="OQS10_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS2293194.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OQS10_002175"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OQS10_002175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS2293195.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OQS10_002176"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OQS10_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS2293196.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OQS10_002177"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OQS10_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS2293197.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OQS10_002178"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OQS10_002178"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS2293198.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OQS10_002179"
     CDS             19086..19745
                     /locus_tag="OQS10_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2293199.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OQS10_002180"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OQS10_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS2293200.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OQS10_002181"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OQS10_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS2293201.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OQS10_002182"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OQS10_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS2293202.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5787 a         6227 c         5794 g         5449 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg actttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg ctgaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacag
//
LOCUS       DAJVXP010000059        23236 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVXP010000059 DAJVXP010000000
VERSION     DAJVXP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.059"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="OQS10_002183"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="OQS10_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCS2293203.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="OQS10_002184"
     CDS             507..1136
                     /locus_tag="OQS10_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCS2293204.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="OQS10_002185"
     CDS             1163..2824
                     /locus_tag="OQS10_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS2293205.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="OQS10_002186"
     CDS             3063..3122
                     /locus_tag="OQS10_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS2293206.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="OQS10_002187"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="OQS10_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS2293207.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OQS10_002188"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OQS10_002188"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS2293208.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OQS10_002189"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OQS10_002189"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS2293209.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OQS10_002190"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OQS10_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS2293210.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="OQS10_002191"
     CDS             9505..10125
                     /locus_tag="OQS10_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS2293211.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="OQS10_002192"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="OQS10_002192"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS2293212.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OQS10_002193"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OQS10_002193"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS2293213.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OQS10_002194"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OQS10_002194"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS2293214.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="OQS10_002195"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="OQS10_002195"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS2293215.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OQS10_002196"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OQS10_002196"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="OQS10_002197"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="OQS10_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS2293216.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="OQS10_002198"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="OQS10_002198"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS2293217.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="OQS10_002199"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="OQS10_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS2293218.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OQS10_002200"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OQS10_002200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS2293219.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="OQS10_002201"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="OQS10_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS2293220.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="OQS10_002202"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="OQS10_002202"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS2293221.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="OQS10_002203"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="OQS10_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS2293222.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6332 c         5907 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc gtgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac cttttc
//
LOCUS       DAJVXP010000060        23004 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVXP010000060 DAJVXP010000000
VERSION     DAJVXP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.060"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            78..1511
                     /gene="cscA"
                     /locus_tag="OQS10_002204"
     CDS             78..1511
                     /gene="cscA"
                     /locus_tag="OQS10_002204"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS2293223.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1519..2577)
                     /locus_tag="OQS10_002205"
     CDS             complement(1519..2577)
                     /locus_tag="OQS10_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2293224.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2745..2963
                     /locus_tag="OQS10_002206"
                     /pseudo
     CDS             2745..2963
                     /locus_tag="OQS10_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2981..4309
                     /gene="dsdA"
                     /locus_tag="OQS10_002207"
     CDS             2981..4309
                     /gene="dsdA"
                     /locus_tag="OQS10_002207"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS2293225.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4417..5955)
                     /gene="emrY"
                     /locus_tag="OQS10_002208"
     CDS             complement(4417..5955)
                     /gene="emrY"
                     /locus_tag="OQS10_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS2293226.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5955..7118)
                     /gene="emrK"
                     /locus_tag="OQS10_002209"
     CDS             complement(5955..7118)
                     /gene="emrK"
                     /locus_tag="OQS10_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS2293227.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7534..8148
                     /gene="evgA"
                     /locus_tag="OQS10_002210"
     CDS             7534..8148
                     /gene="evgA"
                     /locus_tag="OQS10_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS2293228.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8153..11746
                     /gene="evgS"
                     /locus_tag="OQS10_002211"
     CDS             8153..11746
                     /gene="evgS"
                     /locus_tag="OQS10_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS2293229.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11802..12947)
                     /gene="yfdE"
                     /locus_tag="OQS10_002212"
     CDS             complement(11802..12947)
                     /gene="yfdE"
                     /locus_tag="OQS10_002212"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS2293230.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13021..13965)
                     /gene="yfdV"
                     /locus_tag="OQS10_002213"
     CDS             complement(13021..13965)
                     /gene="yfdV"
                     /locus_tag="OQS10_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS2293231.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14035..15729)
                     /gene="oxc"
                     /locus_tag="OQS10_002214"
     CDS             complement(14035..15729)
                     /gene="oxc"
                     /locus_tag="OQS10_002214"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS2293232.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15783..17033)
                     /gene="frc"
                     /locus_tag="OQS10_002215"
     CDS             complement(15783..17033)
                     /gene="frc"
                     /locus_tag="OQS10_002215"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS2293233.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17546..18169)
                     /locus_tag="OQS10_002216"
     CDS             complement(17546..18169)
                     /locus_tag="OQS10_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS2293234.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18466..18741
                     /gene="ypdI"
                     /locus_tag="OQS10_002217"
     CDS             18466..18741
                     /gene="ypdI"
                     /locus_tag="OQS10_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCS2293235.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18818..19060)
                     /locus_tag="OQS10_002218"
     CDS             complement(18818..19060)
                     /locus_tag="OQS10_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS2293236.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19413..20333
                     /gene="lpxP"
                     /locus_tag="OQS10_002219"
     CDS             19413..20333
                     /gene="lpxP"
                     /locus_tag="OQS10_002219"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS2293237.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20689..20760
                     /gene="ypdK"
                     /locus_tag="OQS10_002220"
     CDS             20689..20760
                     /gene="ypdK"
                     /locus_tag="OQS10_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS2293238.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20825..22063)
                     /gene="alaC"
                     /locus_tag="OQS10_002221"
     CDS             complement(20825..22063)
                     /gene="alaC"
                     /locus_tag="OQS10_002221"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS2293239.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22440..>23004
                     /gene="ypdA"
                     /locus_tag="OQS10_002222"
     CDS             22440..>23004
                     /gene="ypdA"
                     /locus_tag="OQS10_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS2293240.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6440 a         5389 c         5024 g         6151 t
ORIGIN      
        1 tatgaaagta agggactata tttttataaa agttaacgtt aacaattcac caaatttgct
       61 taaccaggat gattaaaatg acgcaatctc gattgcatgc ggcgcaaaac gcactagcaa
      121 aacttcatga gcaccggggt aacactttct atccccattt tcacctcgcg ccacctgccg
      181 gatggatgaa cgatccaaac ggcctgatct ggtttaacga tcgttatcac gcgttttatc
      241 aacatcaccc gatgagcgaa cactggggac caatgcactg gggacatgcc accagcgacg
      301 atatgatcca ctggcagcat gagcctattg cgctagcgcc aggagacgat aatgacaaag
      361 acgggtgttt ttcaggtagt gctgtcgatg acaatggtgt cctctcactt atctacaccg
      421 gacacgtctg gctcgattgt gcaggtaatg acgatgcaat tcgcgaagta caatgtctgg
      481 ctaccagtcg ggatggtatt catttcgaga aacagggtgt gatcctcact ccaccagaag
      541 gaatcatgca cttccgcgat cctaaagtgt ggcgtgaagc cgacacatgg tggatggtag
      601 tcggggcgaa agatccaggc aacacggggc agatcctgct ttatcgcggc agttcattgc
      661 gtgaatggac cttcgatcgc gtactggccc acgctgatgc gggtgaaagc tatatgtggg
      721 aatgtccgga ctttttcagc cttggcgatc agcattatct gatgttttcc ccgcagggaa
      781 tgaatgccga gggatacagt taccgaaatc gctttcaaag tggcgtaata cccggaatgt
      841 ggtcgccagg acgacttttt gcacaatccg ggcattttac tgaacttgat aacgggcatg
      901 acttttatgc accacaaagc tttttagcga aggatggtcg gcgtattgtt atcggctgga
      961 tggatatgtg ggaatcgcca atgccctcaa aacgtgaagg atgggcaggc tgcatgacgc
     1021 tggcgcgcga gctatcagag agcaatggca aacttctaca acgcccggtt cacgaagctg
     1081 agtcgttacg ccagcagcat caatctgtct ctccccgcac aatcagcaat aaatatgttt
     1141 tgcaggaaaa cgcgcaagca gttgagattc agttgcagtg ggcgctgaag aacagtgatg
     1201 ccgaacatta cggattacag ctcggcactg gaatgcggct gtatattgat aaccaatctg
     1261 agcgacttgt tttgtggcgg tattacccac acgagaattt agacggctac cgtagtattc
     1321 ccctcccgca gcgtgacacg ctcgccctaa ggatatttat cgatacatca tccgtggaag
     1381 tatttattaa cgacggggaa gcggtgatga gtagtcgaat ctatccgcag ccagaagaac
     1441 gggaactgtc gctttatgcc tcccacggag tggctgtgct gcaacatgga gcactctggc
     1501 tactgggtta acataatatc aggtggaaca acggatcaac agcggacaag ggatccgcgt
     1561 cactcttccc ccttcacgac cttcaataat atgcaatgca gcttcccgcc cgataatgtc
     1621 atgtggaagc tgaattgtgg tcagcggcgg taaaaacaga tgcccgacgc caaccagatt
     1681 atcaaagccc attacggcga catcctgcgg gatacgtacc cccttcgcca ggagaacctg
     1741 ataagcgaca aaggctgcac gatcgttacc acatatcaga acatcaaaat ctggtttgcc
     1801 cgatttgaag tgggcattga gtaaacttgc gagatcggtg tagtgatcat cacctgttgc
     1861 catgtgaaat tgtttcacct cagccagatc tcgtccagca tcacgccagg cctgctcaaa
     1921 tccctgccga cgataccctg ttgccaacgc actttccggt agccagaagc ataacggttg
     1981 acgatagccc gccgcgagca aatgctgtgt tgattcatat tgtgcagtgt aatcatcagg
     2041 gatataactg ggtaacgctg ggtcatccgc cacacagttc gccaatacaa tattttcacc
     2101 atacagagac tcaggcagcg tgatatgtcg cagccccatt gtagtataga taatgccatc
     2161 cggacggtgg gcaagcagct gacgtgccgc gcgggcagcg tcatcttcag aaaaaatatt
     2221 gattaaaaaa ctattccagc cgaactcgct ggcggtttgc tcaatggcaa gcagaatatc
     2281 aacagagaaa ggagtggtag ccgtgtcctg cgccagcacg gcgagagtcg acggcttacg
     2341 tccttgagcg cgcatcttac gggcggaaag atcaggaaca taattcaggg tctggattgc
     2401 ctgcaatacg cggtcacgcg ttgcaggacg cacagattct gcattatgca tcacccggga
     2461 gactgtcatc atcgacactc ccgccaggcg tgcgacatcc tttaatgaag ccatacccaa
     2521 gccgtttgcc gtaaaacggg cactgtagca gaaacagacg tcactggcga gatccaacgc
     2581 cctatcacct gacacagcaa tacaataaaa aataacaata attcccggac aattgtcccc
     2641 aattccgcct ctgttctcgc caacgagtaa ccatataggc aagtaagtaa aacatgccaa
     2701 ctggcatcgc caacctggag aagaaaatat aaatatggat cattgtgctg ccgctgtatc
     2761 ccgacatcag cccggaaatt attgcgattg ctatcggttc aggtgcaatt ggctgcacga
     2821 tcgttacgga ctcgcttttc tggctagtga agcaatattg cggcgctacg ctcaatgaaa
     2881 catttaaata ctatacgaca gcgacattta tcgcttcagt cgtcgctctg gcgggcacat
     2941 tcctgctgtc atttatcatc taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa
     3001 ctcgctcatc gcccagtatc cgttggtaaa ggatctggtt gctcttaaag aaaccacctg
     3061 gtttaatcct ggcacgacct cattggctga aggtttacct tatgttggcc tgaccgaaca
     3121 ggatgttcag gacgcccatg cgcgcttatc ccgttttgca ccctatctgg caaaagcatt
     3181 tgctgaaact gctgccactg gggggattat tgaatcagaa ctggttgcca ttccagctat
     3241 gcaaaaacgg ctggaaaaag aatatcagca accgatcagc gggcaactgt tactgaaaaa
     3301 agatagccat ttgcccattt ccggctccat aaaagcacgc ggcgggattt atgaagtcct
     3361 ggcacacgca gaaaaactgg ctctggaagc ggggttgctg acgcttgatg atgactacag
     3421 caaactgctt tctccggagt ttaaacagtt ctttagccaa tacagcattg ctgtgggctc
     3481 aaccggaaat ctggggttat caatcggcat tatgagcgcc cgcattggct ttaaggtgac
     3541 agttcatatg tctgctgatg cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt
     3601 tacggtcgtg gaatatgagc aagattatgg tgttgccgtc gaggaaggac gtaaagcagc
     3661 gcagtctgac ccgaactgtt tctttattga tgacgaaaat tcccgcacgt tgttccttgg
     3721 gtattccgtc gctggccagc gtcttaaagc gcaatttgcc cagcaaggcc gtatcgtcga
     3781 tgctgataac cctctgtttg tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt
     3841 cgcattcggg cttaaactgg cgtttggcga tcatgttcac tgcttttttg ccgaaccaac
     3901 gcactcccct tgtatgttgt taggcgtcca tacaggatta cacgatcaga tttctgttca
     3961 ggatattggt atcgacaacc ttaccgcagc ggatggcctt gcagttggtc gcgcatcagg
     4021 ctttgtcggg cgggcaatgg agcgtctgct ggatggcttc tataccctta gcgatcaaac
     4081 catgtatgac atgcttggct ggctggcgca ggaagaaggt attcgtcttg aaccttcggc
     4141 actggcgggt atggccggac ctcagcgcgt atgtgcatca gtaagttacc aacagatgca
     4201 cggtttcagc gcagaacaac tgcgtaatac cactcatctg gtgtgggcga cgggaggtgg
     4261 aatggtgccg gaagaagaga tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa
     4321 cgcagcatcg caatcctttc cctgggtgag cgatgctgcc gatggcgcag acttaagatc
     4381 cccggtctta cccgctataa ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa
     4441 cggcggtttc gcaaaccaaa ccaaaaccgt aagtaagata aaagcaatag ccgccatacg
     4501 gaaaatctca tttgcagaaa tagaaagtga ctgctgggtt atttcattat taatttcatt
     4561 aagaactcct gaaagcgaac cataatattt atccataatt tgtgacgatg aattaaacac
     4621 ggggttaaat tgatcgatgg ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc
     4681 ccacagcgtc attgtcaacg acgtaccaac tgatcctgac aaggtacgaa aaaaattact
     4741 catactcgag gcattggcaa atttattatc tggcaagcct gaaaacgaaa tcgttgttaa
     4801 gggtaaaaag aaacaggcaa cggcgaatcc ctgaaaaaac tgcggcaaaa tgatgcctgt
     4861 aaaatcaatc gttggcataa atgtcacaga acgccagtaa tagcaaaccg catacatcaa
     4921 aaaactaaat gtcactaaca accgcatgtc tattttgttg ccataacgtc ctatcaaagg
     4981 tgaaattaat agtggcatga tgccgatggg cgcataagca agtccggccc atatcgcatt
     5041 ataccccatc gtttcctgga gtaactgcgg cataaggacg atcgctccag agtaaaataa
     5101 atacgcgcat gtgatactca caataccaat ggtgaagtta cgggacttaa acaaactgag
     5161 atcaagaatc gggttctctg aggtcgactc ccaaatgact aaagagatca gagaaataac
     5221 tgatactact gttaatatta ttattgtact cgagttgaac caatccagat cgcgcccttt
     5281 atcaagcata atttgcaagc caccaacacc gagcactaac agggtcagtc ctggtagatt
     5341 cattttgacc ggtgaagtct cagtttctct tcctttaagt aaggttaagc ataatgtcag
     5401 gacgataatc cccataggga cattgattaa aaatatccaa ccccagctaa agttatcaca
     5461 aatataaccg cccaatatcg gcccacatat cggagcgata atcacggtca ttgaccataa
     5521 tgccagagca aatgttcttt tttctggcgg ataattcctt aataataaac tctgtgacag
     5581 tggaattaac ggccccgcca ttaacccctg aacgactcta aaaaatatca gcacatcaag
     5641 attggtcgat aggctacaca ttaatgaaga cagcgaaaaa aaggttactg aaagtaaaaa
     5701 taatcttaat tcgcctattc tttgtgccaa cctgccagta acagggatcg caatggcatt
     5761 tgctacacca aacgaggtga taacccaggt gccttcgtct gttgatgctc ccagaaagcc
     5821 agatattgtc ggtattgcga cgttagaaat agtggaatcc aacatttgca taaatgtcgc
     5881 taatgacaat gcaatagtga cgcaccataa cgtcccaccg gttaatggtg ccggagttga
     5941 tttagtgatt gccattaaag ttgtccatta tgcgaaataa tgttgctaat ttctttttct
     6001 atcggactgg tatcgataac taaagcctta ctggtataag ccggcatgga ggtcacggtt
     6061 gaagccagct caggcatctc agcaatgtct tcgtccttcg tatcaatagt tgcagtcatc
     6121 gataaaccaa tgcgcaaggg gtgttccatg agttcttttg gatcaagaga aacttcaacc
     6181 ggtacacgct gaacgatttt gatccagttc cctgtcgcat tttgtgcagg taataaggag
     6241 aacgcattgc cggttcccat attgatccct gtcacccgac catgaaacac aacattttca
     6301 ccataaagat cgctgataat attgactgat tgaccaatcc gtacatccgt gagttgtgtt
     6361 tctttaaagt tggcattaac ccacatttga cgtgccggta cgacagccat taacgattgt
     6421 ccgggactca ctgtttcgcc gacctgaaca cttcgctggg caatatagcc agtaaccgga
     6481 ctccttatat ccgtacgttt aagcgccaac caggcttctt tggttgcatc cgccgcttca
     6541 actacttgtg gctgacggtt taatggtgtg ttcattacta aagctttatt cgctttataa
     6601 gcctggatag cggcattcag tgccgctttg ctacttatta acgtatcttt ggtatgctcc
     6661 agcgtttctt ttgaaataac cccctgcttc gctaaaggca cacgacggtt ataatcttct
     6721 aaggattgtt gatactgaat acgtgctgaa gcgacttcgg cactgtattg tttatcctgt
     6781 aagtacagtt tattcgtttg ccgaacaata tttgccagat tatttttagc tttattgagt
     6841 gcgatagtgg catcagtttt atccagtgaa actaaaatgt caccctgtcg aacgtagttc
     6901 gtatctttat gattaactac agtgacacta cctgagactt gtgcagaaat tggatctgca
     6961 ttccccgtga cataggcgtc atctgtacta atcatgtctt ctaattccat tgaccaatag
     7021 gcataggcac ctgaaaacgc aataaataaa actatcacca ataaagaaaa gtattttttt
     7081 ctgttagact gttttttatt tgaattaatc tgttccacta ttatctctca tttcgcatag
     7141 atgaaattta tgaattgaat gaaaagagta ttcattcttc atatcaaaca cagtgcatgt
     7201 gttgcacatt atcccaggaa cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac
     7261 agaggactaa accgtggctt ttgcaataca attcttacgc ctgtaggatt agtgagaaga
     7321 cttatagtgc taacttgaaa ctataaatca tcggtataat ccctgatttt attgttgaca
     7381 tttcatttat gccgactatt tatatggtat acttgtcgaa ttatcttaaa ggaagctcag
     7441 attttcttat ttttattgag aaaatgagat gacgccttat gtctgtatta ctacagggag
     7501 aagggagatg cttcattgca aagggaataa tctatgaacg caataattat tgatgaccat
     7561 cctcttgcta tcgcagcaat tcgtaattta ttgatcaaaa acgatattga aatcttagcg
     7621 gagttaactg aaggcggaag cgccgttcag cgggtggaaa cacttaagcc tgatatcgtc
     7681 atcattgatg tcgatatccc cggagttaac ggtatcctgg tgttagaaac gctgaggaag
     7741 cgccaatata gcggaattat tattatcgtc tccgctaaaa atgaccattt ttacgggaaa
     7801 cattgtgctg atgctggcgc taatggtttc gtgagtaaaa aagaaggcat gaacaatatc
     7861 attgcggcta ttgaagctgc aaaaaatggc tactgctatt tccccttctc tctcaaccgg
     7921 tttgttggaa gtttaacgtc cgaccagcaa aaactcgact ccttatcgaa acaagaaatt
     7981 agtgtcatgc ggtatattct tgatggcaag gataataatg acattgcgga aaaaatgttc
     8041 atcagcaaca aaactgtcag cacttataaa agtcgcctga tggaaaaatt agaatgtaaa
     8101 tcactgatgg atctttacac attcgcacaa cgtaacaaaa tcggctaacc acatgaagtt
     8161 tttaccctat atttttcttc tctgttgtgg tctttggtcg accataagtt tcgcagacga
     8221 agattacatc gaatatcgtg gcatcagtag taacaaccgt gtcatacttg atccagtacg
     8281 tctgagcaac aaggaattac gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca
     8341 taagtcccaa acggctacgt tgttgcatac cgattcgcag caacgagttc gtggtattaa
     8401 tgctgattat ttaaatcttt taaaaagaac gttaaatatc aaattaacac tccgggaata
     8461 cgccgatcat caaaaagcaa tggacgcgct ggaagaaggt gaagtcgata tagtgttatc
     8521 acatttagta gcttcgccgc ctcttaatga tgacattgct gcaaccaaac cactgataat
     8581 tacctttccg gcgctggtaa ccacccttca cgattcaatg cgaccgctta cctcatcaaa
     8641 accagtaaat attgctcgag tagcaaatta tccccctgac gaggtaattc atcaatcatt
     8701 tccaaaagca acaattatct cttttacaaa tttatatcag gcattagcat ccgtctcagc
     8761 cggacagaat gattacttta ttggtagtaa catcattacc agcagtatga tttcccgcta
     8821 tttcactcac tccttaaatg tagtgaaata ttataactca ccgcgtcaat ataatttttt
     8881 cttgaccaga aaagaatctg tcgttcttaa tgaagtactc aatcgatttg ttgatgcttt
     8941 gacaaatgaa gttcgctatg aagtatcaca aaattggctt gatacaggaa acctggcctt
     9001 tctgaacaaa ccattagaac tcactgaaca tgaaaaacaa tggattaagc agcatcccga
     9061 tttaaaggtg ctggaaaatc cttactcccc tccctattct atgacggatg aaaatggctc
     9121 ggttcggggc gttatggggg atattcttaa tattattact ttgcaaacag gtttaaattt
     9181 ttctccgatc accgtttcac acaatatcca tgctggaaca cagctgaatc ccggcggatg
     9241 ggatatatta cccggtgcta tttatagtga agatagagaa aataatgttt tatttgctga
     9301 agccttcata acaacgcctt acgtttttgt catgcaaaaa gcgcctgaca gtgaacaaac
     9361 attaaaaaaa ggaatgaaag ttgccattcc atattattat gagcttcatt cgcaattaaa
     9421 agagatgtat ccggaggtag agtggataaa agtcgataac gccagcgctg catttcacaa
     9481 ggtcaaggaa ggcgaacttg atgctctggt cgcgacacag ttaaattcac gttacatgat
     9541 cgaccattac tatcctaatg aactttatca ttttcttatt cccggcgttc cgaatgcatc
     9601 gctttcgttc gcttttcctc gcggagaacc ggaacttaag gatattatta ataaagcact
     9661 gaatgcaatc cccccaagcg aagttctgcg cctgaccgaa aaatggatta aaatgcccaa
     9721 tgtgaccatt gacacatggg acctctatag cgagcaattt tatattgtta cgacattatc
     9781 cgttttatta gttggcagta gccttttatg gggattttac ctgttacgct cagttcgtcg
     9841 tcgtaaagtc attcagggtg atttagaaaa ccaaatatca ttccgaaaag cactctcgga
     9901 ttccttaccg aatccaactt atgttgtaaa ctggcaaggt aatgtcatta gtcataatag
     9961 tgcttttgaa cattatttca ctgcggatta ctacaaaaat gcaatgttac cattagaaaa
    10021 cagtgactcc ccctttaaag atgttttttc taatgcgcat gaagtcacag cagaaacgaa
    10081 agaaaatcga acaatataca cacaggtttt tgaaattgat aatggcatcg agaaaagatg
    10141 cattaatcac tggcatacat tatgcaatct gccagccagc gatcatgctg tttatatttg
    10201 tggttggcaa gatattacag agacgcgtga tttaattcat gcactcgaag tagagagaaa
    10261 taaagcgatc aatgcaactg tcgcaaaaag tcagtttctg gcaacaatga gtcacgaaat
    10321 aagaacacca ataagctcca ttatgggctt cctggaactt ctgtcgggtt ctggtcttag
    10381 caaggagcaa cgggtggagg cgatttcact tgcctactcc accggacaat cactcctcgg
    10441 cttaattggt gaaatccttg atgtcgacaa aattgaatcg ggtaactatc aacttcaacc
    10501 acaatgggtc gatatcccta ctttagtcca gaacacttgt cactctttcg gtgcgattgc
    10561 tgcaagcaaa tcgatcgcat taatttgcag cagtacattt cctgatcatt acctggttaa
    10621 gatcgaccct caggcgttta agcaggtctt atcaaattta ctgagcaatg ctctcaaatt
    10681 taccaccgag ggggcagtaa aaattacgac ctccctggtt cacattgatg acaaccacgc
    10741 tgttatcaaa atgacgatta tggattctgg aagtggatta tcgcaggaag aacaacaaca
    10801 actctttaaa cgctacagcc aaacaagtgc aggtcgccag caaacaggat ctggcttagg
    10861 cttaatgatc tgcaaagaat taattaaaaa tatgcagggc gatttgtcat tagaaagtca
    10921 tccaggcata ggaacaacat ttacgatcac aatcccggta gaaattagcc agcaagtggc
    10981 gactgtcgag gcaaaagcag aacaccccat cacactacct gaaaagttga gcatattaat
    11041 cgcggatgat catccgacca acaggctatt actcaaacgc cagctaaatc tattaggata
    11101 tgatgttgat gaagccactg atggtgtgca agcgctacac aaagtcagta tgcaacatta
    11161 tgatctgctt attactgacg ttaatatgcc gaatatggat ggttttgagt tgactcgcaa
    11221 actccgtgag caaaattctt ccttacccat ctgggggctt acagccaacg cacaggctaa
    11281 cgaacgtgaa aaagggttaa gttgcggcat gaacttatgt ttgttcaaac cgttgaccct
    11341 ggatgtactg aaaacacatt taagtcagtt acaccaggtt gcgcatattg cacctcagta
    11401 tcgccacctt gatatcgaag ccctgaaaaa taatacggcg aacgatctac aactgatgca
    11461 ggagattctc atgactttcc agcatgaaac gcataaagat ctacccgctg cgtttcaagc
    11521 actagaagct ggcgataaca gaactttcca tcagtgtatt catcgcatcc acggtgcggc
    11581 taacatcctg aatttgcaaa agttgattaa tattagccat cagttagaaa taacacctgt
    11641 ttcagatgac agtaagcctg aaattcttca gttgctgaac tctgtaaaag aacacattgc
    11701 agagctggac caggagattg ctgttttctg tcagaaaaat gactaaatag cggctcccac
    11761 aatgttctaa tgtgggagct atttaccagc acatcttaca tttatgatga gaactcctgg
    11821 cgaatttgtt ccccatgctg gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg
    11881 cagccgctga tttttatcgg attacccggc atcattattc ctccggcttc aatcaacata
    11941 tttctcgcct gagtttgtgg caaattaatg gcctcagcca cacttaataa cggcgcgacg
    12001 ggcacaccaa cttcatgtat tttggctaac caaacttcag ctgcctgcgt ttttaacgtt
    12061 cgctcaatat attgtttaag aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat
    12121 cggggatcat taaccagttc cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg
    12181 tcattaccac aacaaatcgt aatcggctta tcctgagtat cgaaaacatc aaaaggtgcc
    12241 atgtagggat ggcgatttcc caggcgttgt ggtgacttcc ctgtcgcaat atatgccatc
    12301 agaccatgct ccagaaaact cagcgtggca tcaaacatcg ctatatcgac atgcgcccct
    12361 ctctggctct tttcgcggcc ataaagtgca ctcactattc cgctgaataa atagacaccg
    12421 ccgcatagat ccgcaagaga ggtaccaaca cgcactggcg gagcatcagg gtaccctgtt
    12481 tccatcatta tcccactcat tgcctgaatg atggtatcgt aggcaggagc atcttttagc
    12541 ggaccggtat gtccgaaacc tgacgatgaa gcatatatga ggcgcgggtt gatttcttgt
    12601 aacctttccc atgaaaaccc cagtttttcc attgtacctg ggcgaaaatt ctcagctaat
    12661 acatcagctt gtttgagcat atttataaat atacttttat cgtgatcatt ctttaaatca
    12721 agaaccacac tctctttgcc atgattaata aaactgtaat agagtgactg tccatccaca
    12781 tagggaccaa atgtgcgggt atcatcacca tgacctggtg gctcaacttt aattaccctt
    12841 gcgcccatat tacaaagaag ttgagttccg aaaggtccat taaggacatg tgtcatatcg
    12901 ataactaata agccttcaaa cggccctttg ctttcattat ttgtcatttg cttatgcctt
    12961 gaaaaaagcc gaaggtgaaa agacacttcg gcatttatgt aaaatcaata acatgattaa
    13021 ttatgaaacc agtcgactga cataaatcca caagggagcc gtgacgacaa agcccagtac
    13081 gctcacagcc aatgacgctg taccagtacg ggtgtagaca ttaaaccggc tggcaataat
    13141 gattccggag aatgccggcg gtaatgcgcc tgccagtacc atcatctgca aatgttcgct
    13201 gttcaaatga catgccatac caacgagaag cagcgccagt ggcatcagaa tcaacttcag
    13261 gaaggtgtta taagcaattt cggcactgaa ctcgaattta tgtgcagcca gagtcaaccc
    13321 cgcagcgaat accgctaccc ctgagttagc cttcgcaatc agattaaagg tcgggtccca
    13381 tgctgccgga atttttaccc caaccagcac caggatcgtt gccagaacag gtgcccatac
    13441 tactggctcc tttgccgcag aaattaatgc gctcagatta ctattcttct taccatccgc
    13501 tcctgaggaa ggattcagca aatacagacc aataggaata gtaattgcgt taacaataat
    13561 agaaataatt gctaccacta aacctgttga tacggaatca ccataaatag gatcgagaac
    13621 tgcaaacccc aggaatccaa tggtaggtga acccgcaatt aatgcacata cagcggcttc
    13681 tgcatgggta cgtttaaaaa atttgtagca accaaaccaa gagaaaaaga aacatccgac
    13741 aataaccaca agtgatacaa gggtcagacg agtgtccgca aaaatcattt ccctgtttgc
    13801 ccgggtaata gaaacaaata gagccgcagg aagagcgtag tttaatacca gtttattaaa
    13861 tgcgcgggct tgatcttctg aaaaagtttc tcgtctgccg ctaaaataac ccaacagcat
    13921 gattacgata ataggcaata aatcgccaat aaaaaatgtt agcataatag tatttccgat
    13981 aagggtcccc tcaccatcgc ataatgagtt ggcaagagga gacgatgtca gtatttaatt
    14041 accagcgact tgttttgggt taagtttggt aatatggcca ctttcagttc ctgcagcagg
    14101 gtcgatgacc acattaataa tggtcggttt gcgcgactga ataccggtgg ttaaagcatg
    14161 acgaagttca tctgtcgtgg tgacgttata gccaacgcca cgaaacgcat ccattaattt
    14221 gtcatacctt gcatggtgca acagatccgt tggtgatggt gcaccagcgc cactgagatc
    14281 aacaccgtct cctctgtaga tgccgccatt attaaaaata acgatcgtca ccggcaggtt
    14341 atatcgacaa atcgtttcaa tttccatccc actgaaacca aaagcactat caccttcaat
    14401 ggcgacaacc ggagaaccag aagtcacgct agcaccgatg gcatagccca taccgatgcc
    14461 catgacaccc caggtaccac aatccagacg acgacgtggt ttatacatat caataatatt
    14521 tcgtgcatta tccagggtat ttgcaccttc attaactaaa taaatatcct ggttctcgcg
    14581 caatacgtcg cgcacagcac ttaatgcatt aaagtaattt aatggttgag tatctgtact
    14641 taatttttca tgcatttttt gtgcattttg ctgcttgtgg atatttaaaa tatcgcgcca
    14701 taccagtgga gtcgtaaatg tgttttgttt cagttctgcc agcatacctt gcatactgga
    14761 tgcaatatca ccaacgactg gcacagcaat ggggcggttg ctgtcaattt cctgcggttc
    14821 aatatccagt tgaataaact gtgtatctgc cgcccatcct tttttaccgt gtgccaataa
    14881 ccaattcagt cgtgcaccaa caagcatgac aacgtcagca tttgccaggg caaacgaacg
    14941 cgcagctgcc gcagaaagtg gatgcgtatc ttcaaggatc cctttcgcca tagacattgg
    15001 caggaatgga atctgagcac tttcaataaa ttcacgaagc tgttcatcag cttgtgaata
    15061 cgccgcgcct ttgccaagga taattaatgg ccgttcagct tttgctaaaa gcgaaattgc
    15121 gctagtgact gacttcgggc atggcaataa tgctggcgac ggattttcaa ctttaacaat
    15181 cgtggttaac gcttcgtctt tttccatcgt cgcggccagg acatttgctg gcaaatcaag
    15241 ataaactccg ccagggcgac ccgatacaga gacccggata gcgcgtgcca atgcaatgcc
    15301 aagatcctgc ggctgattaa cgcgaaatgc tgctttggca tacggttttg ccgcattcat
    15361 ttggtccagc tcttcataat caccttgctg taggtcgacg atcgcgcggt cgctggagcc
    15421 gctaatcatg atcatcggaa aaccatttac cgttgcgttg gccaatgcgg tcaaaccatt
    15481 gaggaatcct ggcgcagaaa ctgtcaggca gatccccggt ttttgggtaa gaaaaccgct
    15541 tgccgcagcg gcatagcctg ccgactgctc atgacgaaaa ccaatataac gaatgccttc
    15601 cgcctgggca tggcgtgcca tatccgtcac aggaatacct acaacaccat aaatagtgtc
    15661 aatattattc tgttttaatg cttcaacgat gatatgcata ccatctgtca tttgaagttg
    15721 atctgacatg attaataacc tcgaaatttt tataaattgg gagccagaag cccccgttaa
    15781 tatcagatgg cgtggttttg cttcattgca gcaatttcat cgtcgctata acccagctcc
    15841 tgcaatacag cagcggtatg ttcacctaat agcggcgcag ctttaatatc cggcgtaaag
    15901 gcagagaatt tcattggaca accaacggtc agatattttc cacgcaacgg ttgttccact
    15961 tcaacaacac tgccactttg gcgcaaagag ggatcaagtg aaatttcttt catacttaaa
    16021 accggtgcac aaggaatatc aaactgagtc aaataggcca ccgcttcatg tttatcaata
    16081 gtgacagtgt atttttcgat ttcagcaaaa atatcgaaaa tatgtggctg tcgggcatgg
    16141 gctgtactgt atgccgaatc ggtaatccat tctggtttgc cgatggcttt acaggtgttt
    16201 tcccagtttt gctcctgaat agtgaaataa atataggcgt taggatcggt ttcccagcct
    16261 ttacatttca ggatccagcc aggctgaccg ccaccacctg cattaccacc gcggggaact
    16321 gcatcaccaa atgtaccatt cggatactgc gggtattctt ccagataacc caatttatcg
    16381 agacgctgct ggtcacgtaa tttcacgcgg caaaggttca atacggcatc ctgcattgac
    16441 atggtgactc gttgcccacg ccccgttttt tcgcgatgca gcaaagcagc aagtaaaccg
    16501 atcagcaaat gcattccggt gttgctgtca cccaacgctg cagcgcttac cagcggcgga
    16561 ccatcccaaa aacccgtagt ggatgccgcg ccacccgctg cctgagcaac gttttcatag
    16621 gcttttacat tcacataagg cgaacactca tcaaaccctt tgatcgaacc aaaaatcaga
    16681 cgtggattga tttcttgaat atgctcccag gtgaagccca tgtgatcaat ggcccctgga
    16741 tgaaagttct cgactaagat atcagcttcg cggatcagct tttccattac ctctttgcct
    16801 tccgctgttt tggtatttaa ctcaatagaa cgtttgttac tgttaagcat ggtgaagtaa
    16861 agcgcatcga tatcaggaat atctcgcagt tggtgacgcg ttacgtcacc aacgccggga
    16921 cgttcaattt taataacgtc agcgccaaac caggccagca tttgagtaca agatgggcca
    16981 gattgcacac cggtgaaatc gagaacttta attccttgaa gtggagttga catagcaata
    17041 cctattagtt aattaaatat gtgttaattt agcgtttatt taaggcataa cgccctctat
    17101 aaataatctg caaaatatct tcgtagcggc actcgatatt tttaaattcc tgtatcaagt
    17161 actgaaaatt atttcatcgc cacttccaat tcctttctgc atggtaagta gtgaggacga
    17221 aaagaagaat atgataaata tttattcgtg gtaatcagag agagtcgcga catttgtgat
    17281 gttattctta caactgtaag aagcgccggt gatttacaag taagagtatg attacgcttt
    17341 ttaaagcgta ccataaagca taacataata aatgatttgt gatgacactg gattattgcg
    17401 gtttactttg aggaaaataa aatgcgtgtt tatagaaccg cgagcgccaa ataattttat
    17461 gaccacaacc tgtcgtattg tggtcataaa aaattgtcgg ctggagggag ttaaatcctt
    17521 tacctcaata tactgcctgt cagcattagt tcacaaacag tgcttcgcta tcgacgatga
    17581 ttccatcttc cgcaccttta agcgccaggt ttgcctcgta gtattgcttt ttatcaagca
    17641 gtttctgcgc atcagcaagt gcattacgag tctgtttaag cggcatcaga tattggtttt
    17701 ccattacgcc aacaccggcc aggcgaagct cttccattgc gcccttttta tcccctttcg
    17761 ccattttttc attggcaatt tttatcgcgg cttctttttc gggtgttgct acataactct
    17821 cagaaattcc gacagatgca ttaatgacaa tatattggtc atcattgaga ttggtctttt
    17881 tacctggctt agcgaacttt gcccattcgg tactgtcatc tgacagcaaa gcggaagctt
    17941 cattggtcag ttcttttgcc ttttccggat cgccatgaaa cagagccagg cgcgctacct
    18001 gcacgtcacg catcgcatat aaaccttgtt cagagatacg ctctgctgct gccgttttct
    18061 gggtttgctg agtttgctgt tgtgccgcta ccggtgcatt atcagcagcc catacagttg
    18121 aagatgccag aattgctgtg accatcgtag ccataattaa acgtttcata cttacctcat
    18181 tctttaacag ttttagaaaa cgccgccatc atattgggtg agcatgaaat gagaaatcag
    18241 agagcaacgg tcattttgaa agggttatct tacaattgta gttattatcg caaagcggca
    18301 tttagtctta cagttgtagg aatatgcttc ctgtcgcaga gaactattct tatataccac
    18361 aaagagatgt catttattat attgatgtaa atattgatta attatatgta aatacagcca
    18421 taacatcgta tggttctcag tgaaccattt tcaggataac tttctatgaa agtaaactta
    18481 atacttttca gcttattttt attggtctct attatggcat gcaatgtttt tgcattttcc
    18541 atttctggtg gtgtaagtga gaggagctat aaagagactg aaaaaacatc agcgatgaca
    18601 accacacact ctacaaaact tcagccatca caggcgattt tgtttaagat gagagaagat
    18661 gcgccaccat taaacctcac agaagaaatc acgcctactt atccgacaaa ggcgaattat
    18721 cttattcatc ctgtgcgata gcgtctgtca catggcaggt gaacaatcat gtctgatgct
    18781 gatgtgaaca cctgcccaaa tgagccatcc tgacccacta tcgcctgcca taccccacca
    18841 taaagcgaat catcaagcca caagccgggg caagaaacat aaatatcccc gtcaccagtt
    18901 cattaccgcc agttaaccac atggtgaagt aataaatgag tgcagcgagg atcaccatgc
    18961 cgaagaaaga agagaccgcc gataccacgt tcagtacctg accgatgtag ccgcttacgc
    19021 agacaataca cagggcgaga aacatccata aataaatcat agctcaacct cctgccgcgc
    19081 taaaaaacct gtaacttcag tcactttaat agtaagacaa tccttaacct ctggcaattt
    19141 ttgctaagca tcaatgaaaa cagcacgtta aatgcaacgc tattgcgtgc ggaaaatttt
    19201 ttactttgca cgattaataa tcagtagctg aaagcagtca gccagtgata aagtagagcc
    19261 accgcattca tgctcctcgt ggttatgtcc tgactagtct ttacactctt tacaggaacc
    19321 attgtcgtac atgatggccc aaccaattga gtgtaccgct gtcgacatgg aatcttctgc
    19381 gatacaacaa ttcgtatcta cagaaggtaa ctatgtttcc acaatgcaaa ttttcccgcg
    19441 agtttctaca tcctcgctac tggctcacat ggtttgggct tggtgtactc tggctttggg
    19501 tacagcttcc ttatcctgtt ctctgctttc tcggcacgcg tattggcgca atggcgcgac
    19561 cattcctgaa acgtcgtgaa tctatcgccc gtaaaaacct ggaactttgt ttcccgcagc
    19621 attctgcgga agaacgcgag aagatgattg ccgaaaactt tcgttcactc ggcatggcgc
    19681 tggtagaaac cggcatggca tggttctggc ccgacagtcg cgtacgtaaa tggtttgatg
    19741 ttgaagggtt ggataaccct aaacgcgcac aaatgcaaaa tcgcggcgta atggttgtcg
    19801 gcgtccattt tatgtcgctg gaactgggcg gccgcgtgat gggactgtgc caaccaatga
    19861 tggctaccta tcgtccacat aataatcagc tgatggaatg ggtgcagacc cgtgggcgca
    19921 tgcgctctaa caaagcgatg atcggcagaa ataatctgcg cggcattgtc ggtgcactga
    19981 agaaaggtga agcggtatgg tttgctcccg atcaggatta tggtcgtaaa ggcagctcct
    20041 tcgcgccgtt ctttgcggtg gaaaatgtcg ccacaaccaa tggcacctat gttctctccc
    20101 gtctctctgg cgcagccatg ttgaccgtaa cgatggtaag aaaagcggat tacagcggat
    20161 atcgtttgtt tatcacccca gagatggaag gctacccgac agatgaaaat caagccgctg
    20221 cctatatgaa caagattatc gagaaagaga tcatgcgcgc accggagcag tacctctgga
    20281 tccaccgtcg ctttaaaacg cgcccggtgg gagaatcgtc gttgtacatt taacaaactg
    20341 ctcttactct ttccctcctc aaatcggcca taataacctc atggttttta tggctttttt
    20401 cttaaggttt tttccttcgc tgaatatcta attattaccg tcagaaatta aactgtcgcc
    20461 gatatgagac acttgtaagt tactaaaagt aaataagaaa atgcctcttg tctcgtttca
    20521 gttttaggcg tacattaacg ccgtcttgag cagtttagct cagaattctg gggtgatgcc
    20581 cacggtaaaa tacagggctt ttaaggtcgc tttgcccggt gtcaactcac tgtatcaggt
    20641 gtaatgaagt cattcaggcg taacagtaat tacgcggaga gatgtaaagt gaaatatttc
    20701 tttatgggca tttcttttat ggtcatcgtt tgggccggta cttttgccct gatgatctaa
    20761 aagcaaactg tgcaatgaaa aacaggagcc atcaggctcc tgtttgcatt tctgacgacg
    20821 gtgtttattc cgcgttttcg tgaatatgtt tgctgctggc gggtagtaaa ccgtcggcac
    20881 ggaacatcgc tttaatccct ctgatggcct gacgaatacg gtcgcggttt tcaatcagtg
    20941 caaagcgaac atgcgtatca ccatagtcgc caaagccaat ccccggcgag acacaaacct
    21001 tcgcttcgtt aaggagcttc ttggcaaatt ccagcgatcc catggccgca tatggttccg
    21061 ggattttcgc ccagacatac atcgaagcct tcggcatttc gaccatccag cccgcttcat
    21121 gcagcccttt aaccaataca tcacggcggc gtttgtactg ttcggtaatg tcgcgcacgc
    21181 actgttgatc gccctcgagt gccgcaatcg ccgccacctg caacggcgta aaagtgccgt
    21241 aatcgtgata gcttttgatt cgcgccaggg cactgaccag cgttttgttg ccgaccataa
    21301 agccgatacg ccagcccgcc atgttgtagc ttttcgacag cgtaaagaac tcgaccgcca
    21361 catcgcgtgc acccggcacc tgcataattg acggcgcttt ccagccatcg tagacgatat
    21421 cggcataggc caggtcatgg accaccagca catcgtagcg tttcgccagc gccacaacct
    21481 tttcgaagaa ctccagttcc acgcattgcg cggttgggtt cgacgggaag ccgaggatca
    21541 tcatcttcgg tttcggataa ctttcacgaa tggcgcgctc cagttcgttg aagaaatcga
    21601 caccttccac cagcggcact gagcgtacct gcgccccggc aatcaccgcg ccataaatat
    21661 ggatcgggta acttggattc ggcaccagca ccgtgtcacc atgatccagc gtcgccagca
    21721 tcagatgcgc caggccctct ttcgaaccaa tagtgacgat ggcttctgat tccgggtcga
    21781 tttcaacgtc gtagcgatcc tgataccagc gggaaatggc gcgacgtaac cgcggaatgc
    21841 cgcgtgaagt ggagtaacca tgcgtgtccg ggcgctgggc cacagtacat aatttttcga
    21901 cgatatgcgg cggagtcgca ccgtccgggt tacccatgct gaaatcgata atatcttcgc
    21961 cgcgccgacg cgcagccatt ttcagttcag cggtgatgtt aaaaacgtag ggcgggagac
    22021 gatcaatgcg cgtaaagcga cgttcagggc gagtgtcagc cataatttcc tcagattaac
    22081 gttagcgccc ggaccgtccg agcgacgctg ccacaatggt ggctgttttg aaaatagcct
    22141 gattaatttc tgtctgtcca gcgttttaga acatttttat ccatggcttt aaatgaggaa
    22201 ctccacttca aaaacaggaa atttttctac gctaacaaga aaattaccac caattttatt
    22261 aacattataa tttcaataaa tttaccagcc agaagtgcat tatcaagaga tacattgcta
    22321 atacaccatt tatctttcaa tcccctttta aatttatggt ttttcctcaa aaacggcctg
    22381 ctggtcatca gccttcaggt tacctatcat agaggtttaa tccttattca gagtcacccg
    22441 tgcacgaaat attcaacatg ctgctggcgg tcttcgatcg ggcagcgtta atgcttatct
    22501 gcctgttctt tctcatccgt atccgcctgt ttcgcgaact gttgcacaag tcggcgcact
    22561 ccccaaaaga attgctcgcc gttaccgcca ttttctcgct gttcgccctg ttcagcacct
    22621 ggtccggcgt tcccgtagaa ggctcgctgg tgaacgtacg tattatcgcg gtgatgtccg
    22681 acgggattct gtttggcccg tgggtaggca tcattaccgg cgtgattgcg ggtattcacc
    22741 ggtatttaat tgatatcggc ggcgtgacgg cgatcccctg ctttatcacc agcattctgg
    22801 cgggttgtat atcaggctgg ataaacctga aaatccccaa agcacagcgc tggcgcgtca
    22861 gtattctcgg cggcatgttg tgtgagacgt tgaccatgat tctggtaatt gtctgggcac
    22921 caactaccgc gttggggatc gatatcgtct ctaaaatcgg cattccaatg atcctcggta
    22981 gcgtctgtat cggctttatt gtgc
//
LOCUS       DAJVXP010000061        22592 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVXP010000061 DAJVXP010000000
VERSION     DAJVXP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.061"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="OQS10_002223"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="OQS10_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS2293241.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="OQS10_002224"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="OQS10_002224"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS2293242.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="OQS10_002225"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="OQS10_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS2293243.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="OQS10_002226"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="OQS10_002226"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS2293244.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="OQS10_002227"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="OQS10_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS2293245.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="OQS10_002228"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="OQS10_002228"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS2293246.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OQS10_002229"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OQS10_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS2293247.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="OQS10_002230"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="OQS10_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS2293248.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="OQS10_002231"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="OQS10_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS2293249.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="OQS10_002232"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="OQS10_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS2293250.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="OQS10_002233"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="OQS10_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS2293251.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="OQS10_002234"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="OQS10_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS2293252.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="OQS10_002235"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="OQS10_002235"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS2293253.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="OQS10_002236"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="OQS10_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS2293254.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="OQS10_002237"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="OQS10_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS2293255.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="OQS10_002238"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="OQS10_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS2293256.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="OQS10_002239"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="OQS10_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS2293257.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="OQS10_002240"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="OQS10_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS2293258.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="OQS10_002241"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="OQS10_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS2293259.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="OQS10_002242"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="OQS10_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS2293260.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="OQS10_002243"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="OQS10_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS2293261.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="OQS10_002244"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="OQS10_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS2293262.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OQS10_002245"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OQS10_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS2293263.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22592
                     /locus_tag="OQS10_002246"
     CDS             22225..>22592
                     /locus_tag="OQS10_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2293264.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5058 a         5668 c         6427 g         5439 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat gg
//
LOCUS       DAJVXP010000062        22131 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVXP010000062 DAJVXP010000000
VERSION     DAJVXP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.062"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..524)
                     /locus_tag="OQS10_002247"
     CDS             complement(<1..524)
                     /locus_tag="OQS10_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2293265.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="OQS10_002248"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="OQS10_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS2293266.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1941..2603
                     /locus_tag="OQS10_002249"
     CDS             1941..2603
                     /locus_tag="OQS10_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2293267.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="OQS10_002250"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="OQS10_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS2293268.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /locus_tag="OQS10_002251"
     CDS             3137..3505
                     /locus_tag="OQS10_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS2293269.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="OQS10_002252"
     CDS             3543..3848
                     /locus_tag="OQS10_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS2293270.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="OQS10_002253"
     CDS             3851..4255
                     /locus_tag="OQS10_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS2293271.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="OQS10_002254"
     CDS             4245..4544
                     /locus_tag="OQS10_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS2293272.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4730..5122
                     /locus_tag="OQS10_002255"
     CDS             4730..5122
                     /locus_tag="OQS10_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS2293273.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5192..6205
                     /gene="yqjG"
                     /locus_tag="OQS10_002256"
     CDS             5192..6205
                     /gene="yqjG"
                     /locus_tag="OQS10_002256"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS2293274.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6470..6835
                     /locus_tag="OQS10_002257"
     CDS             6470..6835
                     /locus_tag="OQS10_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS2293275.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="OQS10_002258"
     CDS             7077..7433
                     /locus_tag="OQS10_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS2293276.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="OQS10_002259"
                     /pseudo
     CDS             complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="OQS10_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8485..9186
                     /locus_tag="OQS10_002260"
     CDS             8485..9186
                     /locus_tag="OQS10_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS2293277.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /locus_tag="OQS10_002261"
     CDS             9209..9373
                     /locus_tag="OQS10_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293278.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /locus_tag="OQS10_002262"
     CDS             complement(9507..10817)
                     /locus_tag="OQS10_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS2293279.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQECVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="OQS10_002263"
     CDS             complement(10845..12176)
                     /locus_tag="OQS10_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS2293280.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="OQS10_002264"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="OQS10_002264"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS2293281.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="OQS10_002265"
     CDS             complement(13887..14276)
                     /locus_tag="OQS10_002265"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS2293282.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="OQS10_002266"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="OQS10_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS2293283.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="OQS10_002267"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="OQS10_002267"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS2293284.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="OQS10_002268"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="OQS10_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS2293285.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="OQS10_002269"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="OQS10_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS2293286.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="OQS10_002270"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="OQS10_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS2293287.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21420..21764
                     /gene="tdcR"
                     /locus_tag="OQS10_002271"
     CDS             21420..21764
                     /gene="tdcR"
                     /locus_tag="OQS10_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS2293288.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5801 a         5649 c         5167 g         5514 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaagc gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacactcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt g
//
LOCUS       DAJVXP010000063        21998 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVXP010000063 DAJVXP010000000
VERSION     DAJVXP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.063"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OQS10_002272"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OQS10_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS2293289.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OQS10_002273"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OQS10_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS2293290.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OQS10_002274"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OQS10_002274"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS2293291.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="OQS10_002275"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="OQS10_002275"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS2293292.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OQS10_002276"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OQS10_002276"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS2293293.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OQS10_002277"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OQS10_002277"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS2293294.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OQS10_002278"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OQS10_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS2293295.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8253..8468
                     /locus_tag="OQS10_002279"
     CDS             8253..8468
                     /locus_tag="OQS10_002279"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293296.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="OQS10_002280"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="OQS10_002280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS2293297.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="OQS10_002281"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="OQS10_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS2293298.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="OQS10_002282"
     CDS             11833..12330
                     /locus_tag="OQS10_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS2293299.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="OQS10_002283"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="OQS10_002283"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS2293300.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="OQS10_002284"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="OQS10_002284"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS2293301.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="OQS10_002285"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="OQS10_002285"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS2293302.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="OQS10_002286"
     CDS             15015..15578
                     /locus_tag="OQS10_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS2293303.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="OQS10_002287"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="OQS10_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS2293304.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="OQS10_002288"
     CDS             complement(16169..17149)
                     /locus_tag="OQS10_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS2293305.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="OQS10_002289"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="OQS10_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS2293306.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="OQS10_002290"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="OQS10_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS2293307.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /locus_tag="OQS10_002291"
     CDS             18452..18742
                     /locus_tag="OQS10_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS2293308.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            18750..19343
                     /locus_tag="OQS10_002292"
     CDS             18750..19343
                     /locus_tag="OQS10_002292"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS2293309.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="OQS10_002293"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="OQS10_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS2293310.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21769
                     /locus_tag="OQS10_002294"
     CDS             20783..21769
                     /locus_tag="OQS10_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS2293311.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21814..>21998
                     /locus_tag="OQS10_002295"
     CDS             21814..>21998
                     /locus_tag="OQS10_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCS2293312.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5444 a         5715 c         5596 g         5243 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aagcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgtggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga gaaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga
    21721 tccaaaacaa gctgctttgt tagagaagtt cgacaacgct gctcaataat attccgggcc
    21781 agtctctggc cctgattttc caagatgctt acaatgatat ttaaccgcct gaaacttgcg
    21841 gtagaccgcg tcattgccgc gttttctgtc gccgtcatgc ttgctctggt tgtatgcgta
    21901 gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac cgatgagctg
    21961 gcacgatttt tgatgatctg ggtagggtta ttaggtgc
//
LOCUS       DAJVXP010000064        21552 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVXP010000064 DAJVXP010000000
VERSION     DAJVXP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.064"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..1363
                     /gene="espL1"
                     /locus_tag="OQS10_002296"
                     /pseudo
     CDS             1..1363
                     /gene="espL1"
                     /locus_tag="OQS10_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1535..1642
                     /locus_tag="OQS10_002297"
     CDS             1535..1642
                     /locus_tag="OQS10_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293313.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1695..2453)
                     /locus_tag="OQS10_002298"
     CDS             complement(1695..2453)
                     /locus_tag="OQS10_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS2293314.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2740..3669
                     /gene="pfkB"
                     /locus_tag="OQS10_002299"
     CDS             2740..3669
                     /gene="pfkB"
                     /locus_tag="OQS10_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS2293315.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3770..4060
                     /gene="ghoS"
                     /locus_tag="OQS10_002300"
     CDS             3770..4060
                     /gene="ghoS"
                     /locus_tag="OQS10_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS2293316.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4166..5026
                     /locus_tag="OQS10_002301"
     CDS             4166..5026
                     /locus_tag="OQS10_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS2293317.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5067..5603)
                     /locus_tag="OQS10_002302"
     CDS             complement(5067..5603)
                     /locus_tag="OQS10_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS2293318.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5750..6418
                     /gene="hxpB"
                     /locus_tag="OQS10_002303"
     CDS             5750..6418
                     /gene="hxpB"
                     /locus_tag="OQS10_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS2293319.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6581..7171
                     /locus_tag="OQS10_002304"
     CDS             6581..7171
                     /locus_tag="OQS10_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS2293320.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7304..8695
                     /gene="tcyP"
                     /locus_tag="OQS10_002305"
     CDS             7304..8695
                     /gene="tcyP"
                     /locus_tag="OQS10_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCS2293321.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8699..9515)
                     /locus_tag="OQS10_002306"
                     /pseudo
     CDS             complement(8699..9515)
                     /locus_tag="OQS10_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9792..10055)
                     /gene="cedA"
                     /locus_tag="OQS10_002307"
     CDS             complement(9792..10055)
                     /gene="cedA"
                     /locus_tag="OQS10_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS2293322.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10238..12499
                     /gene="katE"
                     /locus_tag="OQS10_002308"
     CDS             10238..12499
                     /gene="katE"
                     /locus_tag="OQS10_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS2293323.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12757..13515)
                     /gene="chbG"
                     /locus_tag="OQS10_002309"
     CDS             complement(12757..13515)
                     /gene="chbG"
                     /locus_tag="OQS10_002309"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS2293324.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13528..14880)
                     /gene="celF"
                     /locus_tag="OQS10_002310"
                     /gene_synonym="chbF"
     CDS             complement(13528..14880)
                     /gene="celF"
                     /locus_tag="OQS10_002310"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS2293325.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14985..15827)
                     /gene="chbR"
                     /locus_tag="OQS10_002311"
     CDS             complement(14985..15827)
                     /gene="chbR"
                     /locus_tag="OQS10_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCS2293326.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15835..16185)
                     /gene="chbA"
                     /locus_tag="OQS10_002312"
     CDS             complement(15835..16185)
                     /gene="chbA"
                     /locus_tag="OQS10_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS2293327.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16236..17594)
                     /gene="chbC"
                     /locus_tag="OQS10_002313"
     CDS             complement(16236..17594)
                     /gene="chbC"
                     /locus_tag="OQS10_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS2293328.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTATPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17679..17999)
                     /gene="chbB"
                     /locus_tag="OQS10_002314"
     CDS             complement(17679..17999)
                     /gene="chbB"
                     /locus_tag="OQS10_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS2293329.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18298..18636)
                     /gene="osmE"
                     /locus_tag="OQS10_002315"
     CDS             complement(18298..18636)
                     /gene="osmE"
                     /locus_tag="OQS10_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS2293330.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18838..19665
                     /gene="nadE"
                     /locus_tag="OQS10_002316"
     CDS             18838..19665
                     /gene="nadE"
                     /locus_tag="OQS10_002316"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS2293331.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19895..20782
                     /gene="cho"
                     /locus_tag="OQS10_002317"
     CDS             19895..20782
                     /gene="cho"
                     /locus_tag="OQS10_002317"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS2293332.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20742..21317)
                     /gene="ves"
                     /locus_tag="OQS10_002318"
     CDS             complement(20742..21317)
                     /gene="ves"
                     /locus_tag="OQS10_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS2293333.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5737 a         5313 c         4939 g         5563 t
ORIGIN      
        1 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
       61 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
      121 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
      181 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
      241 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
      301 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
      361 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
      421 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
      481 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
      541 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
      601 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
      661 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
      721 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
      781 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
      841 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
      901 aacaaaaaaa atggtgatga gtattttagc catgaaaata catgatggtg agccaggatt
      961 atacgccgca atggaaaata atcacccttt gtgtgtcaca cggttcctct ctaaaattaa
     1021 tggcatcgcc tttaaataca agttgagcaa agctaacatc atggatttat taaaaggagc
     1081 tacagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg aagacgtcgt
     1141 gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta gtcaacatca
     1201 gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc atatagccat
     1261 tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg tcatcagcca
     1321 aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta tttacgatat
     1381 atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca tgtgcacaac
     1441 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tatccctttt gttacactcc
     1501 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
     1561 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
     1621 ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg cataacacgg
     1681 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
     1741 tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
     1801 aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt atcttccgcg
     1861 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
     1921 ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
     1981 acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca
     2041 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
     2101 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
     2161 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
     2221 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
     2281 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
     2341 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
     2401 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
     2461 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
     2521 attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaataactta gcaatgtatt
     2581 cttatttcat tttttgaata agcatgtggc gaaaacagat ttttatttat atatatttat
     2641 ctgcaaaatt ttaaataaag ctccaataaa tcatattgtt aatttcttca ctttccgctg
     2701 attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat ctatacgttg
     2761 acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc cgaaggaaaa
     2821 ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt cgcccgcgcc
     2881 attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc gaccggcgaa
     2941 cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga agccaaagac
     3001 tggacccggc agaatttaca tgtacatgtg gaagcaagcg gtgagcagta tcgttttgtt
     3061 atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca agttctggaa
     3121 attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt gaagctggaa
     3181 aaattaaccc aactgatttc cgcttcgcaa aaacaaggga tccgctgcat cgtcgacagt
     3241 tctggcgaag cgttaagtgc agcactggca attggtaaca tcgagttggt taagcctaac
     3301 caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccggacga tgtccgcaaa
     3361 gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc ccttggtcca
     3421 caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc accggtgaaa
     3481 agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact gaaactggca
     3541 gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag tgcagccaca
     3601 ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta cgcttacctt
     3661 tcccgctaac aaaaacattc ccccagcatt gggggaatca ccaccaacct gtcggcaacg
     3721 cgtttctccg actttgctca aaagtcatgt gataacaaag gggtgaacta tggccagtgg
     3781 cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga ctgaaattaa
     3841 cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg atgatgaggg
     3901 aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa gtgaagaaga
     3961 aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc ctgaaatcac
     4021 catcacgact tgggaggaat ggaatagcaa cagaaaataa atggtttttg ggcaataatc
     4081 agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa ccttatgatc
     4141 tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt tgagcgagca
     4201 gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag tccatgccgc
     4261 atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa gggaactgct
     4321 tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta aaaccgtcac
     4381 cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg tgatggatta
     4441 tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc aaattgcgcg
     4501 tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact cgctctccac
     4561 aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg aacaacggat
     4621 tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata tcgacgccat
     4681 cgtcgagcat attcagcagc gtctggcctc gcatcaactg cagccttctc tgttgcacgg
     4741 cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt tcgaccctgc
     4801 ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata ctgaacagcc
     4861 gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt tccttgaacg
     4921 tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg gcggtcagca
     4981 tttggttatt gctcagcagt cattggatag attattagca gcatgatatg ggttgaggat
     5041 aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa gaacacataa
     5101 cctgcagcaa tacaaataac cggcaggata tatagtgaga aaaactgcag gaaaatagta
     5161 tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc ttcccctttg
     5221 gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc ctggcgactc
     5281 atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa aatcacccag
     5341 aacataacgt tcacgccacc attaaaatta ggggtcgggg agttatacca gaacagattc
     5401 aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc gagcatgacc
     5461 gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa agaaatcaat
     5521 gtggagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa aacagcaatg
     5581 cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt ttcacttagt
     5641 ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg cgcttaatca
     5701 taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa tgtcaacccc
     5761 gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact cagaaccttt
     5821 acgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct cccgtcgtaa
     5881 cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt ggtacgcccg
     5941 gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg cccgtgccat
     6001 ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagctg ttgcgttatg
     6061 caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata tgctggaaaa
     6121 agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg ctgaaaaact
     6181 gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac tgggcgttga
     6241 cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt ctaaagcagc
     6301 ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac gttttgtatt
     6361 agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc tcggttaatg
     6421 accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt caactgacgg
     6481 attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca ctgtataaaa
     6541 atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg aaggtcacct
     6601 tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc ccgtgctggc
     6661 acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt taccggacat
     6721 cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac cgatcgcccg
     6781 cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc tgctggcaac
     6841 cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac aaggaatggt
     6901 gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg ttcccctgct
     6961 ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg gcaaccaact
     7021 ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc attcattacc
     7081 cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga tccagtttca
     7141 tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt ctggaataag
     7201 caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc caacactatg
     7261 actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact ttccattaat
     7321 tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc gccataaaca
     7381 gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt ttggccttgc
     7441 cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac agtggtttaa
     7501 catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt tagtcttcgc
     7561 ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca aaatcagttt
     7621 tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg gtgtgctggt
     7681 caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag aaactgcacg
     7741 tctgaacgcc attgaaagta actatgttgg taaagtctct gatctgagcg ttccgcagct
     7801 ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca atccgacgtc
     7861 aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga aactgctgaa
     7921 ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac aaagctgggt
     7981 gatgaaactg gttcgccttg tcatgcagtt gaccccttac ggcgttctgg ctctaatgac
     8041 caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt tcgttgtcgc
     8101 gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg gcattaatgg
     8161 cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg cctttaccag
     8221 ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc gtctgggcgt
     8281 tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc agaacggctg
     8341 cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg gcattaaccc
     8401 gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt ccgcaggcgt
     8461 tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg cgatgggcct
     8521 gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca tgggccgtac
     8581 ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt ggctgaagca
     8641 aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc gttaattctt
     8701 atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa tgttccatcg
     8761 cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg tccaacttat
     8821 caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc tcatacccga
     8881 gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca ccacttccca
     8941 ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat attatcgaag
     9001 gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc ggcaggatgt
     9061 atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct attttttata
     9121 ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat cccaacaaaa
     9181 ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat tatcactcta
     9241 tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggagctcc tggctttgag
     9301 gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga ctctgctatt
     9361 tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat ggtagactca
     9421 agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc ataattactc
     9481 tgtattaagt ttgtagaaaa catctcccgc cccatattgt taacaaaatt attatctcat
     9541 ttaaatctaa gtcacttaca atataagttt aagagcgacg ccacaggatg aactatcaaa
     9601 aatagctcat catgattagc aaaacttaac cattttaaaa taaataaaca attaaagaaa
     9661 aaagatcact tatttatagc aatagctcgt caaaggcagc tttttgttac aggtggtttg
     9721 aatgaatgta gcaacgaaat gcagaatttc aggtcatgta actcccggca aaaccgggaa
     9781 gtatgtaatc cttactcagt cacttcccct tcctggcgga tctgatttgc ccaacgttgg
     9841 gcagattcag gcacagtaaa cgccggtgag cgcagaaatg actctcccat cagtacaaac
     9901 gcaacatatt tgccacgcag catccagaca tcacgaaacg aatccatctt tatcgcatgt
     9961 tctggcggcg cgggttccgt gcgtgggaca tagctaataa tctggcggtt ttgctggcgg
    10021 agcggtttct tcattactgg cttcactaaa cgcatattaa aaattagaaa aactgtagtt
    10081 tagccgattt agcccctgta cgtcccgctt tgcgtgtatt tcataacacc gtttccagaa
    10141 tagtctccga agcgggatct ggctggtggt ctatagttag agagtttttt gaccaaaaca
    10201 gcggcccttt cagtaataaa ttaaggagac gagttcaatg tcgcaacata acgaaaagaa
    10261 cctacatcag caccagtcac cactacacga ttccagcgaa gcgaaaccgg ggatggactc
    10321 actggcacct gaggacggat ctcatcgtcc agcggctgaa ccaacaccgc caggtgcaca
    10381 acctaccgcc cctgggagcc tgaaagcccc tgatacacgt aacgaaaaac ttaattctct
    10441 ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg gcgtgcgcat
    10501 cgccgatgat caaaactcac tgcgtgccgg taaccgtggt ccaacgctgc tggaagattt
    10561 tattctgcgc gagaaaatca cccactttga ccatgagcgc atcccggaac gtattgttca
    10621 tgcacgcgga tcagcagctc acggttattt ccagccatat aaaagcttaa gcgatatcac
    10681 caaagcggat ttcctctcag atccgaacaa aatcacccca gtatttgtta ggttctctac
    10741 cgttcagggt ggcgctggct ctgcagatac cgtgcgtgat atccgtggtt ttgccaccaa
    10801 gttctatact gaagagggta tttttgacct cgttggcaat aacacgccaa tcttctttat
    10861 ccaggatgcg cataaattcc ccgattttgt tcatgcggta aaaccagaac cgcactgggc
    10921 aattccacaa gggcaaagtg cccacgatac tttctgggat tatgtttctc tgcaacctga
    10981 aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca gttaccgcac
    11041 catggaaggc ttcggtattc acaccttccg cctgattaat gccgaaggga aggcaacgtt
    11101 tgtacgtttc cactggaaac cactggcagg taaagcctca ctcgtttggg atgaagcaca
    11161 aaaactcacc ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag ccattgaagc
    11221 aggcgatttt ccggaatacg aactgggctt ccagttgatt cctgaagaag atgaattcaa
    11281 gttcgacttc gatcttctcg atccaaccaa actgatcccg gaagaactgg tgcccgttca
    11341 gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac ttctttgccg aaaacgaaca
    11401 agcagcattc catccaggtc atattgttcc tggtctggac ttcaccaacg atccgctgtt
    11461 gcagggacgt ttgttctcct ataccgatac acaaatcagt cgtcttggtg ggccgaattt
    11521 ccatgagatt ccgattaacc gtccgacctg cccttaccac aacttccagc gtgatggcat
    11581 gcatcgtatg gggatcgaca ctaacccggc gaattacgaa ccgaactcga tcaacgataa
    11641 ctggccgcgc gaaacaccgc cgggaccgaa acgcggcggt tttgaatcat atcaggaacg
    11701 cgtggaagga aataaagttc gtgagcgcag tccatcgttt ggcgaatatt attcccatcc
    11761 gcgtctgttc tggctaagtc agacgccatt cgagcagcgc catattgtcg atggtttcag
    11821 ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg accagctggc
    11881 gcatattgat ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg aactgactga
    11941 cgaccagctg aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg atccatcctt
    12001 aagtttgtac gccattcctg acggtgatgt gaaaggtcgc gtggtagcga ttttacttaa
    12061 tgatgaagtg agatcggcag accttctggc cattctcaag gccctgaagg ccaaaggcgt
    12121 tcatgccaaa ctgctctact cccgaatggg tgaagtgact gcggatgacg gtacggtgtt
    12181 gcctatagcc gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg tcattgtccc
    12241 ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc aactactacc tgatggaagc
    12301 ctacaaacac cttaaaccga ttgcgctggc gggagacgcg cgcaagttta aagcaacaat
    12361 caaggtcgct gaccagggtg aagaagggat tgtggaagct gacagcgctg acggtagttt
    12421 tatggatgaa ctgctaacgc tgatggcagc acaccgcgtg tggtcacgca ttcctaagat
    12481 tgacaaaatt cctgcctgat gggaggcggc gcaattgcgc cgcctcaaat gatttacata
    12541 gtgcgctttg tttatgccgg atgcggcgtg aacgccttat ccggcctaca aaactgtgca
    12601 aattcaatat attgcaggaa acacgtaggc ctgataagcg aagcgcatca ggcagttttg
    12661 cgtttgtcag cagtctcaag cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga
    12721 tgcaaggttc acgccgcatc tggcaaacat cctcacttac acatcccgat aactccccaa
    12781 ccgataacca cgctgagcga tagcaccttt caacgacgct gatgtcaaca catccagctc
    12841 cgttaagcgt gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg
    12901 acacatcacc tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa
    12961 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag
    13021 attagccggt aaatcaccag agttagatag cggctgacgg tcgatgcgca acgcaatccc
    13081 ctcttcagcc gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg
    13141 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
    13201 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
    13261 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
    13321 agtaaggaca aagtacatcc ctatggccag actcggttca tcacgactca actgcaccgc
    13381 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
    13441 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
    13501 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
    13561 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
    13621 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
    13681 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
    13741 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
    13801 cttacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
    13861 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
    13921 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
    13981 cgggttttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc
    14041 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata
    14101 aagcaaataa gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag
    14161 atcgaaaata tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa
    14221 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
    14281 gccgaacaaa tcgatagata aatcatcgct gtctttcagc atcagaacat cgtgaataaa
    14341 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
    14401 ataaacggct tcagtaacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
    14461 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
    14521 aaacagaccg cccgcgccgt tggtttcctg gccaagataa ccatgactta atggaatacg
    14581 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
    14641 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
    14701 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
    14761 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
    14821 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
    14881 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
    14941 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
    15001 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
    15061 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
    15121 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
    15181 ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc
    15241 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
    15301 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
    15361 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
    15421 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
    15481 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
    15541 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
    15601 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
    15661 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
    15721 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
    15781 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
    15841 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
    15901 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
    15961 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
    16021 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
    16081 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
    16141 ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc atcatacatc gttcctctct
    16201 attcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc gttagcgata
    16261 tcttcttcgc gctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag
    16321 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg caaccagcaa tgcggcgacg
    16381 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
    16441 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
    16501 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
    16561 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
    16621 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
    16681 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
    16741 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
    16801 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
    16861 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
    16921 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
    16981 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
    17041 cgcgatactg aagcaggtac gctatcgggc aatttaatga cccaattgcg gcggacaata
    17101 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgctcca
    17161 cctaaccagt ttgcgccaac cacataggcc tcaccgacac tatatggggt ggcggtcata
    17221 aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc
    17281 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
    17341 ttgccgccaa tacctttcag gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata
    17401 cctaaggaat aaaaaaacgc cccctcccca aagcttagaa aaacgttgtt aattaataca
    17461 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
    17521 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
    17581 ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg cggtattgat
    17641 ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta
    17701 atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag
    17761 tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca
    17821 atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc
    17881 ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat
    17941 accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata
    18001 aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc
    18061 catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg
    18121 gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg
    18181 ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca
    18241 aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta
    18301 cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac
    18361 atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag
    18421 gatgtaggtc tggcaagtac cacgagcatg gatcatgctc acttcagacg aaggtttacc
    18481 cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg
    18541 tacaaactgg tctttagtac gatcataagc cgtacaaccc gccagcatgg ttaataccgc
    18601 cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg
    18661 tgttagataa gcctggaata cattgggcgc tttttcaagc ccgcgaacga aacggctccg
    18721 ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt
    18781 tcagtggcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg
    18841 acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag
    18901 gaaattcgtc gtagtatcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa
    18961 tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag
    19021 atggcgatta atgagctgcg cctggaaacc ggcaacgaat cactgcaatt tattgccgta
    19081 cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat tgcctttatt
    19141 caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggccag cgaacaggca
    19201 ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag
    19261 cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat
    19321 catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg tacagacatt
    19381 aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt tactggcggc attaggttgc
    19441 ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg cccttctctg
    19501 ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa
    19561 aacgtacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa
    19621 cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt tgcacataat
    19681 ggtttgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattcgac
    19741 ctacaaaact atgcaaattc aatatattgc aggagtcgaa taggcctgac aggcgtagca
    19801 cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctc cccgcctgtt
    19861 acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg
    19921 gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt cattccttaa
    19981 tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct
    20041 ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga
    20101 tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgtaccg caggcgaaat
    20161 cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg
    20221 gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt
    20281 gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag
    20341 gcgcgcagct ttgcaagcat tgcagaccat cgctgatgaa caaaaacttt gttatggcct
    20401 gctgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg
    20461 cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc
    20521 tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca
    20581 gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa
    20641 ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta
    20701 taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc
    20761 gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc
    20821 cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag caatttatcg
    20881 cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc aaaggtagta
    20941 aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgtgt catgatgttg
    21001 aaatccatcg acatctgccc ttccgtcagt ttcgctttta ccacctggtc cgctgcaaag
    21061 gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc
    21121 tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga aaactcccca
    21181 ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa
    21241 gtgcaaattt cgcgcgtttc accggcagca tttcgccaca gattcaccga cattttacgc
    21301 atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa
    21361 ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac
    21421 aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag
    21481 tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag ttgcaggcat
    21541 tttacctttt gc
//
LOCUS       DAJVXP010000065        21164 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVXP010000065 DAJVXP010000000
VERSION     DAJVXP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21164
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.065"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQS10_002319"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OQS10_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS2293334.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="OQS10_002320"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="OQS10_002320"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS2293335.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQS10_002321"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OQS10_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS2293336.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="OQS10_002322"
     CDS             complement(3717..4511)
                     /locus_tag="OQS10_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS2293337.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="OQS10_002323"
     CDS             4649..4990
                     /locus_tag="OQS10_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS2293338.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQS10_002324"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OQS10_002324"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS2293339.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="OQS10_002325"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="OQS10_002325"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS2293340.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="OQS10_002326"
     CDS             8906..10198
                     /locus_tag="OQS10_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2293341.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="OQS10_002327"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="OQS10_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS2293342.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="OQS10_002328"
     CDS             complement(10731..11189)
                     /locus_tag="OQS10_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS2293343.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="OQS10_002329"
     CDS             complement(11186..12103)
                     /locus_tag="OQS10_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS2293344.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="OQS10_002330"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="OQS10_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS2293345.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="OQS10_002331"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="OQS10_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS2293346.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQS10_002332"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OQS10_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS2293347.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQS10_002333"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OQS10_002333"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS2293348.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQS10_002334"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OQS10_002334"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS2293349.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="OQS10_002335"
     CDS             16063..16749
                     /locus_tag="OQS10_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2293350.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="OQS10_002336"
     CDS             16746..19160
                     /locus_tag="OQS10_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2293351.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQS10_002337"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OQS10_002337"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS2293352.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..>21164)
                     /gene="allS"
                     /locus_tag="OQS10_002338"
     CDS             complement(20557..>21164)
                     /gene="allS"
                     /locus_tag="OQS10_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS2293353.1"
                     /translation="VAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTG
                     TEALANTFSLDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKR
                     VAWRLPGQKEIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPS
                     PLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
BASE COUNT         4904 a         5570 c         5699 g         4991 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acgg
//
LOCUS       DAJVXP010000066        21135 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVXP010000066 DAJVXP010000000
VERSION     DAJVXP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.066"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OQS10_002339"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OQS10_002339"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS2293354.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OQS10_002340"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OQS10_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS2293355.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OQS10_002341"
     CDS             complement(2058..2672)
                     /locus_tag="OQS10_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS2293356.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OQS10_002342"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OQS10_002342"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS2293357.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGQQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OQS10_002343"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OQS10_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS2293358.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OQS10_002344"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OQS10_002344"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS2293359.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7745..8446)
                     /gene="fre"
                     /locus_tag="OQS10_002345"
     CDS             complement(7745..8446)
                     /gene="fre"
                     /locus_tag="OQS10_002345"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS2293360.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8493..9986)
                     /gene="ubiD"
                     /locus_tag="OQS10_002346"
     CDS             complement(8493..9986)
                     /gene="ubiD"
                     /locus_tag="OQS10_002346"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS2293361.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10153..10641
                     /gene="rfaH"
                     /locus_tag="OQS10_002347"
     CDS             10153..10641
                     /gene="rfaH"
                     /locus_tag="OQS10_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS2293362.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10638..11420)
                     /gene="tatD"
                     /locus_tag="OQS10_002348"
     CDS             complement(10638..11420)
                     /gene="tatD"
                     /locus_tag="OQS10_002348"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS2293363.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11462..12238)
                     /gene="tatC"
                     /locus_tag="OQS10_002349"
     CDS             complement(11462..12238)
                     /gene="tatC"
                     /locus_tag="OQS10_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS2293364.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12241..12756)
                     /gene="tatB"
                     /locus_tag="OQS10_002350"
     CDS             complement(12241..12756)
                     /gene="tatB"
                     /locus_tag="OQS10_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS2293365.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12760..13029)
                     /gene="tatA"
                     /locus_tag="OQS10_002351"
     CDS             complement(12760..13029)
                     /gene="tatA"
                     /locus_tag="OQS10_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS2293366.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13108..14748)
                     /gene="ubiB"
                     /locus_tag="OQS10_002352"
     CDS             complement(13108..14748)
                     /gene="ubiB"
                     /locus_tag="OQS10_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS2293367.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14745..15350)
                     /gene="ubiJ"
                     /locus_tag="OQS10_002353"
     CDS             complement(14745..15350)
                     /gene="ubiJ"
                     /locus_tag="OQS10_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS2293368.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15364..16119)
                     /gene="ubiE"
                     /locus_tag="OQS10_002354"
     CDS             complement(15364..16119)
                     /gene="ubiE"
                     /locus_tag="OQS10_002354"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS2293369.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16214..17641)
                     /gene="rmuC"
                     /locus_tag="OQS10_002355"
     CDS             complement(16214..17641)
                     /gene="rmuC"
                     /locus_tag="OQS10_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS2293370.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17782..18543)
                     /gene="udp"
                     /locus_tag="OQS10_002356"
     CDS             complement(17782..18543)
                     /gene="udp"
                     /locus_tag="OQS10_002356"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS2293371.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18805..19617
                     /locus_tag="OQS10_002357"
     CDS             18805..19617
                     /locus_tag="OQS10_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS2293372.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20011..21072
                     /gene="hcp"
                     /locus_tag="OQS10_002358"
                     /pseudo
     CDS             20011..21072
                     /gene="hcp"
                     /locus_tag="OQS10_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5195 a         5668 c         5301 g         4971 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aattagcttg atcataaaac gatcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg cgagttcatc agaaacttcc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcataatttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgaag caaaggcaga aaagtcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacag ccggagatca gcaggaagat agcaatgata gtgttaatag tcgggctgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc agaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttacggt tcggccacca
     1861 cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgatgata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagaggtaat ttccccgacg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcctgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctttttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat acgaatgccg ccgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcaatgaag tagatacccg gttcgatggt taacaccata cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgctggcc cagcggatga ccgataccat
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc agtttggcga tgcgctgatg gaactggatg tggtaatcca
     3121 catagctaac gcctgctttc atggtggcga tcagcgccag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttgcat atttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc gaaacgccag ccgaagctgg aaccgctaaa
     4861 actgagcaag attgaagcca ccatgagctt caccatcgct aaaggaatgg tcgcgcaaac
     4921 ggcggggaaa cattatccgg cccccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcct taaatctgga aaacaaaagt tttgtcccgc tggcgcatac
     5041 caacgaagct cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacccc gaaacaggct gctgtgctgg gcgcaggcat
     5161 catgggtggc ggtatcgctt accagtccgc gtggaaaggc gtgccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgagcgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccggat ttgaccgcgt ggatattgtg gtagaagcgg ttgttgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc aggataccgt
     5461 gctggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcgggatgc acttctttaa cccggtccac cgaatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gccgatgggc ccggcatatc tgctggacgt
     5821 tgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tgcgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctggcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcaggt ctggttggcg gcgtggagca tatgggccat gtgccgatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaagtgat
     7021 tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaag
     7081 cggtacggta acggcgggta catcttctgc actttctgat ggtgccgcgg ccatgctggt
     7141 gatgagtgaa agccgcgccc gtgaattagg tcttaagccg cgcgcccgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc ccggttcctg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggtt gttccggtgc
     7441 gcgtatcagc accacgttgc tgaatctgat ggaacacaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggttttta
     7741 tatctcagat aaatgcaaac gcatcgccaa acagacgatc ttcccgcgca ttacgctcac
     7801 tgcaaaacag gtcacgagca attttcgcca tctcaaaacg tccggcaata tagatatcat
     7861 gctctgccag cgtaccatga tcctgcaaga ccgccgttaa cacggtgcca gtacgcccac
     7921 gccagcccgc ttccggttgt tcaaccaccg gcaccacttg cagaccagga tgcttcagcg
     7981 aaagcgcctc aagctcgcag agatcataca gatgttgctc ttcacgccca ccccagtaaa
     8041 tggtgatatc gcgattggga ttacgcgcca acgccgtcaa taaaatcgag cgcgcatagg
     8101 agaacccggt gccgcccgca atcaaaatca tcggacgctc ttcatcatcg cgcagccacg
     8161 cttctccgtg gggaatatcg accacgattt gatgatcttt gaggatgcgg tccatgactg
     8221 ctttcgcgta aaggttgatt tcagaagcgc caatatgcag ctcgataaac cctttttcat
     8281 ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc atccatcact accatcaaat
     8341 actgaccagc acgaaaagaa aaggccgcgt ctggtacgat tcggacacgg tatacagtat
     8401 ccgtgatagc ttctaccgag gtcactttac agcttaaggt tgtcatgcgc tttctctgtc
     8461 ggatcaaata aatagggcaa aactaacgcg catcaggcgc ttttaccgtt gttaaaaata
     8521 gccagttcat cccagatggc gtcaatatgc gcgacgacat caggatcttt tttgatggga
     8581 cgtccccatt cacgctgggt ttcccccggc catttattcg tggcatccag ccccattttt
     8641 gaacccagcc cggagacagg cgaggcaaaa tccagataat caataggcgt attttctacc
     8701 agaacagtat cccgcgccgg gtccatacgg gtggtaatcg cccaaatcac atcgttccag
     8761 tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt tagtgtacat aaactggcgt
     8821 aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc cggcgtactg ttttttgatt
     8881 gtcactaccg ccagacgata agaacagcct tccggtggca ggtaaaaatc gacaatttcc
     8941 gggaactgtt tttgcagaat cggcacaaac acttcgttca gtgcgacacc cagcaccgcg
     9001 ggttcatccg gcggacgacc agtataggtg gagtggtaaa tcgcatcttc acgctgggta
     9061 atatgcgtca cggtaaatac cgggaaacta tcgacttcgt tatagtaacc ggtgtggtcg
     9121 ccatacggcc cttccggcgc agtttcgcct tgttcgatat acccttccag cacaatctcc
     9181 gcactggcgg gcacttcaag gtcattggag atacacttca ccacttcggt cttggtaccg
     9241 cgcagcaatc cggcaaacgc atactctgaa agtgtatccg gaacgggggt gactgcaccg
     9301 agaatcgtgg cgggatcggc acccagcgcc acagaaaccg ggaaacgttc gcccggatgc
     9361 gccgcacacc actcctgata atccagcgcg ccgccgcgat gcgacagcca gcgcataatc
     9421 agtttgtttt taccaatcag ctgctggcga taaatgccca gattctgccg ctctttatgc
     9481 gggccgcgcg ttacggtcag cccccaggta atcagcggcg cggcatcttc cggccagcag
     9541 gtcataatgg gaatgcgatt gagatcgacg tcatcgccag agacgatttt ttgttggcag
     9601 ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt gcttaaactg cggcagttta
     9661 tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt tcagaaacgc caataattta
     9721 ccaacttcac gcagcgccga aacatcttcc tgccccatgc ccatcgccac gcgctttggc
     9781 gtaccgaaca ggttgcacag caccggcatt gagtagcctt tagggttttc gaacaacagc
     9841 gcaggcccac cggcacgcaa agtgcggtca gcaatttcag tgatttccag atgcggatcc
     9901 accgggagcg tgatacgttt tagctcaccc tgctgttcaa gcagcgtcaa gaagtcgcgt
     9961 aaatcgttat atttcatggc gtccattgta gcctcttaat ctacgcccat tatacggcgt
    10021 tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa ctctgacggt ataacgcaaa
    10081 ccggggaata taattaactt agcgtaaagc ttttgctatc cttgcgcccc gattaaacgg
    10141 ataagagtca ttatgcaatc ttggtattta ctgtactgca agcgcgggca acttcaacgt
    10201 gcccaggaac accttgagag acaggctgtg aattgcctgg caccgatgat caccctggaa
    10261 aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat tgtttcccaa ctacctgttt
    10321 gtcgaatttg atccagaagt gattcatacc acgactatca acgcgacccg cggtgtcagc
    10381 cacttcgtgc gctttggcgc gtcgctagcg atagtcccat cggcggtgat tcatcagcta
    10441 tcggtatata aaccgaaaga cattgtcgat ccggcaaccc cttatccggg agataaggtg
    10501 attattaccg aaggcgcgtt cgaaggcttt caggccattt tcaccgaacc cgatggtgag
    10561 gctcgctcca tgctattgct taatcttatt aataaagaga ttaagcacag tgtgaagaac
    10621 accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg ttttgacatt agcatccgtg
    10681 gtggcagcca gccatgcggc atcttctcca cgccagtgcg caatacgttg caaaatatgg
    10741 ggcagatggg ctggctcgtt gcgccgggat gatggctttg gcgtgagatc gcgagggagc
    10801 agatacggcg catcagtttc gatcagtaat ttttccgccg gaatcaacgg caaaagttcc
    10861 cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg taatgccgat ataaattcca
    10921 tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa agcaatgaag aaccgcacca
    10981 ggcagtttat ccagccacgg ctccagcaat gtcataaacc gctcgtgggc atcgcgacag
    11041 tgcataaata ccggcatgtt taaatctgcg gcaatgcgta gctgggcaac aaaagcgcgt
    11101 tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga gaccacattc accaatcgcc
    11161 accacttctg gctgcgcggc cagctcaata atcgcttctt cagtcgcagc ttgccactgg
    11221 ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg acgaatactg acgcgccagc
    11281 ttttgcgcct gctggctttc acgcaggtta gtgccggtga tgagtagccc attaactccc
    11341 gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg caaattgcga actggtcaaa
    11401 ttaacgccga tatcaaacat cctgtactcc atatgacaac cgccctgacg ggcggttgaa
    11461 tttattcttc agttttttcg ctttctgctt cagcgtcgtt ttcctcttcc cgatttcgcc
    11521 ctttaccaac gtaaaagcgt gagaagaaga caccgatttc aaacagacag tacatcggga
    11581 tcgccaatag cgtttgcgag aagacatccg gcggcgtcag caacatcccg acaacgaatg
    11641 caccaaccag cacatacggg cgttttttgc gtaagtcttc tggcgaggta atccccatcc
    11701 agcacagcag cacaattgcc accggcactt caaaggagac accaaacgcc ataaacagcg
    11761 ccataacgaa gcttaaatag ctggcgatgt cggtggatac ctgcacccct tccggcgcgg
    11821 tattggcaag gaagccaaat gccagcggaa agaccacaaa gtaggcgaat gccatgccga
    11881 tataaaacag cagagagctg gaaaccagca gcggcaccac caggcgacgt tcatgcttat
    11941 acagcgctgg ggcgataaat gcccacacct gatagagaat caccggcgct gacagaatca
    12001 gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg cgaggccacg tcggtggcga
    12061 tcatcgttga accttgcggc aactgcttga tcaatggcgc ggataccagg tgatagatgt
    12121 cattggcgaa atagaccaga cacaggaata tcacgatcac cgcgataatg cagttcagca
    12181 gacgcttacg cagctcaatt agatgcgtga taagcggttg agtatcttct acagacatgt
    12241 ttacggttta tcactcgacg aaggggaagg tgcagcggtt ttcggttcag cgtccgcagc
    12301 aggttttacc accggctctg gcgtggtttc tggcttctgt tccggcgaac tggcctgcgt
    12361 ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca ttatctttca ccaccgggtt
    12421 atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg tttgcaacgt aggaacgctt
    12481 catcgactcc gcggcctggc gtagttcatc catcgacgct ttcagttcgg gcgtcaggtt
    12541 agtgaggctc gccttttcaa cctttttcag actgtcctga aactcctgga gttttaactc
    12601 ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc aacgcgcgaa tccagcccgc
    12661 taccgttttt accgccacag gcagtcgttg cggccccaga acgacgaggc cgatgatgaa
    12721 caccaatagc agttcgctaa aaccgatatc aaacacggat tacacctgct ctttatcgtg
    12781 gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta tccgcctgct tatcggcgat
    12841 agttttcgca gtaaaatcag catcctgact ggttttatcc tgctttggtt catcatcgct
    12901 cattgctttt ttaaagcctt tgatcgacgc accaagatcg gaaccgatgg agccgagctt
    12961 tttggtgcca aaaagcagta caacgatgac ggcaataatc aataactgcc aaatactgat
    13021 accacccata catgttcctc tgtggtagat gatgattaaa caaggccgca ttatacgtta
    13081 cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg cgccaaccga caaaccaggc
    13141 gatcagacca cctgccatta accagccggg catcagcccc cattcaggtc ggctgaccaa
    13201 caagaatgtg ccacttaata ctaacgtagc gccaattccg agaaaataac gcgattgtcc
    13261 ctgacgtaca tgatttgact gaagctcgcg ggcaatctta tcaacactgt gctgtaaata
    13321 cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt tctggcattt tttcgaccca
    13381 gaacggcgct ttttctttaa atgctctcac cagggcagga ataccgacct gatctttaat
    13441 ccacgactcc aggaaaggct tcgctgtttt ccataaatcg agctgcggat aaagctggcg
    13501 tcctacccct tcgacataga gcagggtttt ctggagtaac accagttgcg gctgcacttc
    13561 catattgaag cgacgcgccg tattaaacag atttaacagt acatgtccaa acgaaatttc
    13621 ggccagcggt ttctcaaaga taggttcaca gaccgtacga atggcaaatt cgaactcttc
    13681 aacgttggta tctggtggca cccagccaga atcgacgtgt agctctgcca ctttgcgata
    13741 gtcgcgatta aagaaggcga taaagttttc tgccagatag cgtttatctt ctttgtttag
    13801 cgagccaaca atcccgcaat caatgccgat atatttcggg ttttccgggt gttcatagct
    13861 tacgaaaatg ttgccagggt gcatatcggc atggaaaaag ctgtcgcgaa agacctgagt
    13921 gaagaacacc tgcacgccac gttccgccag caatttcatg ttggtgccat ttttctccag
    13981 cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc atcaccatca tcccttcact
    14041 acaatagtca gggtaaactt ccgggatgta gagcatcggg ctgtcttcaa aatttcgccg
    14101 aagctgaatg gcgttggcag attcccgcag caaattcagt tcatcaatca aggtcttttc
    14161 gtactcgcgc accacttcgg ttgggcgcag acggcgacca tccggcagca aacgcggcac
    14221 ccagcgagcc agacggtaga taagtttcag atccgcttta ataaccggca aaatatccgg
    14281 gcggatgact ttaatcacca cctctttacc attcgatttc aatcgcgcgg tatgaacctg
    14341 ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg tcaaaccacg cttctaccgg
    14401 caagccgccc attgcagctt caatctgctg cttcgccagc ttgccatcaa acggagcaac
    14461 tttgtcctgc aataacgcca gctgatcggc aatatgcggt ggaaaaagat cgcggcgggt
    14521 tgataacatt tgcccgaact tgatccaaac cggccccagt tcttgcaggg ccagtcgtag
    14581 tcgctcacct aaaagtttgt ctttatgccg atttggcatc cagaataatg agtatcgcca
    14641 tagccgtagc ggcagggtga tacgcatttt ggggatcagt tcatcaagtc cgtagcttaa
    14701 aaaagtgcga atgatgaaat ataggcgccg tacttcacct ggcgtcattt agcctccagt
    14761 ttttccagcc gtttggtcag ggcatcaaca gcacgctcga cggcagccgt ctcttccgca
    14821 aaccaggcca cttcaagcgg accgggtgcc atacgccact cttcagtaat ggcttccgcc
    14881 acataacgtt gctggcgctt aatgccgtga tgcaggaact ttgcgcctcc gcgcatggct
    14941 ttgctgattc cttcagcggc gatatcaccg gtataagggg ccagcagttc cgcagggtcg
    15001 aactctgcca gatctgccag cgcaacgaag ttttgcacca cctgaatatc gccctgcact
    15061 tccagctcac cactgcgaat cagtgcggta agctgctggc gatcgcgaag tttcggcaac
    15121 acactggcgt aggcgataac ggtgcagtca gcatcgcctg cccattcgcc cagtacatca
    15181 acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa agccttttac ctccacgcgc
    15241 aatactttac ccagcagacg cgagcgggct gttttcagcg cgggtgagcg atacaggaag
    15301 gtgttgagca gactttcaat tcctgccgtc actaaaggtt taaaaggcat ttccggtctc
    15361 ctgtcagaac ttataaccac gatgcagcgc cacaacccct gccgtcagat tgtagtagtc
    15421 gacactttcg aatccggcat cctgcatcat ggctttcagg gtatcctgat cgggatgcat
    15481 acggatggat tctgccagat aacggtagct gtcggcgtcg ttcgcgacca gtgagccaat
    15541 acgcggcagc acatggaagg agtatgcatc ataggctttg ctcagcggct cgataattgg
    15601 cttcgagaac tcaagcacca gcaggcggcc gccgggtttc agcacgcgat acattgaacg
    15661 cagtgcttta tctttgtcgg tgacgttacg cagaccaaac gaaatggtga tgcaatcaaa
    15721 ggtgttatcc gggaacggca gcgcctcagc gttcgcctga acatactcaa cgttgccaat
    15781 cacaccgata ttacgcagct tctcgcgtcc cattttgagc atggattcat tgatatcagc
    15841 aaggaccact ttgccagttt ctccgaccag gcgggagaat ttcgctgtca ggtcgccggt
    15901 gccaccagcc agatccagca cggtctgccc acggcgtacg ccgctgcaat caatcgtgaa
    15961 tcgcttccac aaacgatgaa taccaaatga catcaaatca ttcatgacat cgtattttga
    16021 tgccacggaa tggaaaacgt gggcgaccat atccgctttt tgttccttcg cgacggtctg
    16081 aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc tcaatgcctg ctcatcaaaa
    16141 aattgttcca gaagtgtaac agattgggcg tcgatgccct agatttctac ccggcttaac
    16201 tacccctaat gggctagcgc gactgctgat tatattcatc atcgcgttga taagcttcat
    16261 cattcggctg ctccggaacc gaccgaagtc gatactcttc attctggctc accgcctgtt
    16321 cagccaaatc cggattaatc tcgcgtttaa tttctactcc taaaccgcga aacgcttctg
    16381 cctgcgccag cacatttccg cgacctgaag agagtttttt cattgcctgg cggtagttat
    16441 cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc atcgatgaac aaacgcatct
    16501 tgtcgtacag cttgctggca cgatcggcga tttgctgggc gttgcggctt tgatgctcat
    16561 aacgccacag gttggcaata gtacgcaacg ccaccagcag cgtagtcggg ctaaccagca
    16621 tgatattgtt tttcaacgct tcggtgatca gctccggctg gcggtcaagc gccagtaaaa
    16681 aagcgggttc aacgggaata aacatcagca cgtaatccag agttcgcagc cccggcagct
    16741 gttgataatc tttgcgtccc agcaaacgga tatggttacg caccgacgcg atatgttcct
    16801 gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa atagcgttca taggcgacca
    16861 gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg cagccgcacg atgacatccg
    16921 gctgcatccg cgagcgggcg tcattttcga tgctgacctg ggtttcatat tcataccctt
    16981 cacgcagacc ggaagcctcc agcacccgcg tcaatactac ctcgccccag ttgccctggg
    17041 ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc ttcctgggcc atttgcgcgt
    17101 tgagttgctg gagattgcga atttcgtggg tcagggtatg gcgttcttgt gcttctttac
    17161 cgaagctgtc ctgaacctga cggcggaaac cgtccagttg ttcacgtagc ggcgacaaca
    17221 ggctgttcag actctgacgg ttttgctcat caacccggcg attgctgtgc tcaaaaatac
    17281 ggttggcgag gttttcaaac tgctcactga ggcgctgctc gctgttaatc atctggcgaa
    17341 ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt ggttacttca cgcagatcgg
    17401 cctccagaga ggtgttaata ctttgcaggc tgcgcacttc gttattgagt aactcgcact
    17461 ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc cgcgcttaac tccgcgacca
    17521 tctcttcacg ttcagctaat tgctcggctt tttgctgcgc atgttgataa ctggcaaaca
    17581 gccagccaat tgccacaccc accaacgcaa taactgcgta aaccatgatt gagaaatcca
    17641 caacgcctcc tgacgcaaca acttacccgc acaaaataga attgtgctgt acaaacgtcc
    17701 agttggaaag gcatttcctg cgaggcgcta cgcaaaaata caaaaggccg aacgcgtcgg
    17761 ccttcagaca ggagaagaga attacagcag acgacgcgcc gcttccacca cgattttcac
    17821 cgcttggctt tcagtttgtt tcatcgtctc agcattcggg atctcttgct gggtgcggtt
    17881 aacgataaca cccgctacca taccggcacg caggccctga cttgcacaca tggtcagcag
    17941 ggttgcagat tccatttcat agttcattat gcccatcgcc tgccactctt ccatagaacc
    18001 tttaaagtga cgaactacgc gaccagagta agtatcgtaa cgttcctgac ctgggtagaa
    18061 ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg ccaatggatt tcgcagcttc
    18121 aaccagcgca gtcgtacatt cgaaatcagc gacagccggg aattccagcg gtgcgaagtg
    18181 caggctcgcg ccatccagac ggacagacgc cgtggtaacc aggacatcac ccacattaat
    18241 atgcggctga atagcgcccg ttgtaccgat acgcaggaag gtgcgaatgc ccagctgtgc
    18301 cagctcttca acagcaatag aggtagacgg gccgccgata ccggtagagc agacgataac
    18361 aggtttacca tccagctctg cacgccaggt agtgaattcg cggtgagatg ccagcttaac
    18421 cggcttatcc atcagcgcgg cgatcttttc cacacgatcc gggtcgccag ggacgatggc
    18481 aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga tgaaaaacat cagacttgga
    18541 catatacaac tcctctgtga atcggtttag tcagaagaag caaaaagaca ctttaccgaa
    18601 gggtttaaca ttttttcgtg atactcatca ccatgacgca aatgcgttgc ataaatttac
    18661 ccaccaaaag tgattcaaat cacataaatc actcttcatt ctgcaggact gcataaaaga
    18721 tgagccgatt caggcaatgt gatttgttgc atgctgacta tagttaacat tgcgcaacat
    18781 ttttataagg gtacggagaa taccatggca acaacacaac aatctggatt tgcacctgct
    18841 gcatcgcctc tcgcttcgac catcgttcag accctggacg acgcgattgt ggcgggcttc
    18901 acctctatcc cttcacaagg ggataacatg cctgcttacc atgccagacc aaagcaaagc
    18961 gatggcccac tgccagtggt cattgtagtg caggaaattt ttggcgtgca tgaacatatc
    19021 cgcgacattt gtcgccgtct ggcgctggag gggtatctgg ctatcgcacc tgaactttac
    19081 ttccgcgaag gcgatccgaa tgattttgcc gatatcccca cgttgcttag cggtctggta
    19141 gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc atgccgccag ttgggcttcc
    19201 cgcaacggcg gcgatgttca tcgtttaatg atcaccggat tctgctgggg tggacgtatc
    19261 acctggctgt atgccgcgca taatccacag ctaaaagctg cagtggcgtg gtacggcaaa
    19321 ctgacgggcg acaagtcgct gaattcaccg aaacaacctg ttgatatcgc aaccgatctt
    19381 aacgcgccga ttctcggctt atatggcggt caggataaca gcattccgca ggagagcgtt
    19441 gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag cagagattat cgtgtacccg
    19501 gatgccgggc atgcattcaa cgctgattat cgcccgagct atcatgccga atctgcgaaa
    19561 gatggctggc agcgtatgct ggaatggttt aagcagtatg gagagaagcg gagttagttt
    19621 cctcttagtc tatatttgag atggtgagta aacgagtgcg ttttgctgcc atctctgtcc
    19681 cccaaaaaat aatcagtatg aattgattgg agattacgac tccgctattt gaccttaaga
    19741 aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg aagacacctt agagatttac
    19801 catcaactcc cattcacaaa caaacattaa ttattatcaa cacattcaca ttaattataa
    19861 atcatctcag accttcctga taaaaagata ttaaagtgaa acattttgtt tttcacattt
    19921 tagttcatca cttttaggcg taaataaagg taaaaaaaac aacaatacta tttctctttc
    19981 acaaaagaat taacaaaagg tttttcaagg atgagtgata ttatttacct gaccataacc
    20041 ggagaacaac aaggatgtat ttcctctcgt tgtggaacga gcgcatcaat aggtaatcgc
    20101 tggcagattg gccatgatga tgaaatcttc gcgttctcgc tctctaatag tattacgaat
    20161 acaggcaaag ggtcataatt gcatggcctt agtttctgta aattgattga caaaagctcc
    20221 ccgttactta ttaacgctat taacaataac gagcagttgt tcatggagtt tgacttctat
    20281 cggatcaata ggtttggtcg atgggagaaa tattacaaca ttcaactccg cggcgcgctc
    20341 ttatcagcaa tcaatcacct gtttactgag aacaatctgg atacagaagc catcactgtc
    20401 agttattaat atatactctc caggcatctg attgctaata ctgaatttag ttattgggca
    20461 tttcccgata actacaaccg tctgtttata ccacggccaa aaactaaagc tggtaatggt
    20521 ttcaaaacac ttaatagtaa agcggttggc agactgcttg ctgctgtcgg catatacaat
    20581 gggaatatcg aaggattcag ggaaacagca gacaagttgg ggggagatgc acctgctggt
    20641 tatgatcaat ttatggataa caaagggtta ctcatcgctg gtgcttcaat agttgctgga
    20701 gcagttctgg ggagaatgag actccctgat ttggaatcac ttgagcattt cggagccaga
    20761 ggagcagtat caggaagacc atttaatccc gagcttgccg gtggtccaat agaaaacctg
    20821 acgacagatg gcgtaacaat caacagggaa ggtatcgcta ttgttgaaaa acatatagcc
    20881 agatttggtc atgatcctgt aaatgaagtg atgatcaata gactgaaaga cattgagaaa
    20941 ggaaacatcc ccccagaaca agtagatctt aacttctata ctcatgagtg tcgggaatat
    21001 cagcgttact gtaatcttgg ttgggaaaca ggacaacctg atggtgacgc aggctatgca
    21061 ctctggaatc atactggatt tgcccctata tttccagaca tctgttatca cttaacccat
    21121 tacaagcccg ctgcc
//
LOCUS       DAJVXP010000067        21003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVXP010000067 DAJVXP010000000
VERSION     DAJVXP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.067"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..170)
                     /locus_tag="OQS10_002359"
     CDS             complement(<1..170)
                     /locus_tag="OQS10_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293373.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="OQS10_002360"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="OQS10_002360"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS2293374.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="OQS10_002361"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="OQS10_002361"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS2293375.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="OQS10_002362"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="OQS10_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS2293376.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OQS10_002363"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OQS10_002363"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS2293377.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OQS10_002364"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OQS10_002364"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS2293378.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="OQS10_002365"
     CDS             4288..5151
                     /locus_tag="OQS10_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS2293379.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="OQS10_002366"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="OQS10_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS2293380.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="OQS10_002367"
     CDS             6489..7106
                     /locus_tag="OQS10_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS2293381.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OQS10_002368"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OQS10_002368"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS2293382.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="OQS10_002369"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="OQS10_002369"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS2293383.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="OQS10_002370"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="OQS10_002370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS2293384.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="OQS10_002371"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="OQS10_002371"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS2293385.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="OQS10_002372"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="OQS10_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS2293386.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="OQS10_002373"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="OQS10_002373"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS2293387.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OQS10_002374"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OQS10_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS2293388.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="OQS10_002375"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="OQS10_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS2293389.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="OQS10_002376"
     CDS             18072..19781
                     /locus_tag="OQS10_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS2293390.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OQS10_002377"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OQS10_002377"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS2293391.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5714 c         5385 g         4821 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccacg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAJVXP010000068        20764 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVXP010000068 DAJVXP010000000
VERSION     DAJVXP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.068"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(166..576)
                     /locus_tag="OQS10_002378"
     CDS             complement(166..576)
                     /locus_tag="OQS10_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCS2293392.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="OQS10_002379"
     CDS             complement(634..867)
                     /locus_tag="OQS10_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCS2293393.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="OQS10_002380"
     CDS             1256..1801
                     /locus_tag="OQS10_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCS2293394.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="OQS10_002381"
     CDS             1776..3701
                     /locus_tag="OQS10_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS2293395.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="OQS10_002382"
     CDS             3698..3904
                     /locus_tag="OQS10_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCS2293396.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="OQS10_002383"
     CDS             3901..5502
                     /locus_tag="OQS10_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS2293397.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="OQS10_002384"
     CDS             5483..6802
                     /locus_tag="OQS10_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS2293398.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="OQS10_002385"
     CDS             6812..7144
                     /locus_tag="OQS10_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS2293399.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="OQS10_002386"
     CDS             7200..8225
                     /locus_tag="OQS10_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS2293400.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="OQS10_002387"
     CDS             8267..8665
                     /locus_tag="OQS10_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS2293401.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="OQS10_002388"
     CDS             8677..9030
                     /locus_tag="OQS10_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCS2293402.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="OQS10_002389"
     CDS             9042..9620
                     /locus_tag="OQS10_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2293403.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="OQS10_002390"
     CDS             9617..10012
                     /locus_tag="OQS10_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2293404.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="OQS10_002391"
     CDS             10020..10760
                     /locus_tag="OQS10_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2293405.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="OQS10_002392"
     CDS             10776..11198
                     /locus_tag="OQS10_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS2293406.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="OQS10_002393"
     CDS             11180..11614
                     /locus_tag="OQS10_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS2293407.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="OQS10_002394"
     CDS             11607..14168
                     /locus_tag="OQS10_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS2293408.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="OQS10_002395"
     CDS             14165..14494
                     /locus_tag="OQS10_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2293409.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="OQS10_002396"
     CDS             14494..15192
                     /locus_tag="OQS10_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS2293410.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="OQS10_002397"
     CDS             15198..15941
                     /locus_tag="OQS10_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2293411.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="OQS10_002398"
     CDS             15878..16510
                     /locus_tag="OQS10_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS2293412.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20050
                     /locus_tag="OQS10_002399"
     CDS             16571..20050
                     /locus_tag="OQS10_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS2293413.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            20118..20717
                     /locus_tag="OQS10_002400"
     CDS             20118..20717
                     /locus_tag="OQS10_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS2293414.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT         4774 a         5153 c         6411 g         4426 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacatcgac cgtctgcgcc tgaccttcgg
    16921 tgtgcaggca ctggtggaaa ccacctcaaa gggtgaccgg aatccgtcgg aagtccgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccact tcgcagtatc tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagga tgacaccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcgtacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgct
    17221 ggtcggcgtg caggtggatt cagagcagtt cggtaaccag caggtgagtc gcaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgc ttaagccagc atacagcaac aacatggcct ggtgtctgtg
    17401 ggacatgctc actcatccgc gctacggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 caaatgggcg ctgtatgtca tcggccagta ctgcgaccag tcagtgacgg acggatttgg
    17521 cggcacggag ccgcgcatca cctgtaactc ttacctgacc acacagcgta aggcgtggga
    17581 tgttctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg gacagatgct
    17641 gacgttcgtg caggaccgac cgtcggataa ggtgtggacc tataaccgca gtaatgtggt
    17701 gatgccggac gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa
    17761 tgccgttgag gtgaactgga ttgacccaga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg cccgttacgg tcgtaatgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgagc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggaaacgcag accgtggact tcagcgtggg cgcagaaggg cttcgccatg taccgggtga
    18001 tgtcattgaa atctgcgatg atgattatgc cggtatcagc accggtggtc gcgtactggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg
    18121 taccacgctg ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg tgaaagtgag ccgggttcct gacggcgttg cagaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgcg ccagcgtctg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc
    18361 catcgtggat aacggggcac actttgacgg tgaccggcgc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcatc tgaccgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcga tgggacacgc cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac
    18541 cgtggcagcg gacgacggca gtgagcggct ggtcagcaca gccaggacga cggaaaccac
    18601 ataccgcttc aggcagctgg cgctggggaa ttacagtctg acagtccggg cggtaaatgc
    18661 ccgggggcag cagggcgatc cggcgtcggt atcgttccgg attgcggcac cggcagcgcc
    18721 tgtcactatt gaactgatac cggggtattt tcagataacg gcggtcccga aactggctgt
    18781 atatgacccg acggtgcagt ttgagttctg gttttcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accagcgccc gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcagg ccgggccatg attattattt ttacgttcgc agtgtgaaca ccgttggcaa
    18961 atcggcattt gtggaggcca tcggtcgggc gagcgatgat gcagaaggtt atctgaattt
    19021 ttataaaggg ttgatcaata aaacgcatct cggcaaggag ttgtggacgc agattgataa
    19081 cggtcagctt gcgccggacc tgactgaaat caggacgtcc attacgaatg tcagcaatga
    19141 aatcacgcaa accgtcaata aaaaactgga aaatcagagt gctgcaatcc agcagataca
    19201 gaaagttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcaaca
    19261 gatgaaggac ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc
    19321 aggaatgcag agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa
    19381 tggcaacaca aagccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaatgc ctgacggctc cgaccattac cagcggcggt aatcctccgg cattttccct
    19501 gacacctggc ggacggctga cggcgaaaaa tgccgatatc agcggtaacg tgaacgcgaa
    19561 ctccgggacg ctcaacaacg tcacgattaa taagaactgt cgggttctgg gaaaattgtc
    19621 cgcgaaccag attgaaggcg atctcgttaa aacagtgggc aaacctttcc cacgggactc
    19681 ccgggcaccg gagaggtggc catcagggac cattaccgtc agggtttatg acgatcagcc
    19741 gtttgatcgg caaattgtta ttcccgcggt ggcgtttcgc ggtgctaaac atgagcggaa
    19801 gaataacaat atttattcgt catgccgcct gatagtgaag aaaaacggtg ctgaaattta
    19861 taaccgaacg accctggata atacgctgat atatacgggt gttattgata tgcctgccgg
    19921 tcacggtcac atgacgctgg agttttctgt atcggcatgg ctggtaaatg gctggtatcc
    19981 cacagcaagt atcagcgatt tgctggttgt ggtgatgaag aaagccactg caggcatcac
    20041 gattagctga attttataac ccagatacgg gcaccagaaa tggtgccttt tttattgcag
    20101 aaaagcgaga ggtaattatg cgtaaagttt gtgcagccat tttgtccgca gccatctgtc
    20161 tggccgtatc cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg agcgccgggt
    20221 atcttcagac ccatactgat atgccaggca gcgatgacct gaagggcatt aacgtgaaat
    20281 accgttatga gtttacggac acgctggggc tgattacgtc attcagttat gccaacgctg
    20341 aagatgagca aaaaacgcat tacagcgata cccgctggca tgaggattcc gtgcgtaacc
    20401 gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg
    20461 cgatggcggg cgtggcttac agccgtgtgt cgaccttctc cggggattat ctccgcgtaa
    20521 ctgacaacaa ggggaaaacg cacgatgtgc tgaccggaag tgatggcggt cgccacagca
    20581 acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccattg
    20641 accttgctta tgaaggttcc ggcagtggtg actggcgcac tgacggtttc atcgtgggtg
    20701 tcggttataa gttctgatta gccaggtaac acagtgttat gactgcccgc tggttcaggc
    20761 gggc
//
LOCUS       DAJVXP010000069        20711 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVXP010000069 DAJVXP010000000
VERSION     DAJVXP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.069"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OQS10_002401"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OQS10_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS2293415.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OQS10_002402"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OQS10_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS2293416.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OQS10_002403"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OQS10_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS2293417.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OQS10_002404"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OQS10_002404"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS2293418.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OQS10_002405"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OQS10_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS2293419.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OQS10_002406"
     CDS             complement(8214..9107)
                     /locus_tag="OQS10_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS2293420.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OQS10_002407"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OQS10_002407"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS2293421.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OQS10_002408"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OQS10_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS2293422.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OQS10_002409"
     CDS             complement(10238..11470)
                     /locus_tag="OQS10_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS2293423.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OQS10_002410"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OQS10_002410"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS2293424.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OQS10_002411"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OQS10_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS2293425.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OQS10_002412"
     CDS             complement(14275..16539)
                     /locus_tag="OQS10_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCS2293426.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OQS10_002413"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OQS10_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS2293427.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OQS10_002414"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OQS10_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS2293428.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OQS10_002415"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OQS10_002415"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS2293429.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OQS10_002416"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OQS10_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS2293430.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAJVXP010000070        20693 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVXP010000070 DAJVXP010000000
VERSION     DAJVXP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.070"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            75..1463
                     /locus_tag="OQS10_002417"
     CDS             75..1463
                     /locus_tag="OQS10_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS2293431.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="OQS10_002418"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="OQS10_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS2293432.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="OQS10_002419"
     CDS             complement(2010..2114)
                     /locus_tag="OQS10_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293433.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="OQS10_002420"
     CDS             2284..2730
                     /locus_tag="OQS10_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS2293434.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OQS10_002421"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OQS10_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS2293435.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="OQS10_002422"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="OQS10_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS2293436.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="OQS10_002423"
     CDS             4841..5188
                     /locus_tag="OQS10_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS2293437.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OQS10_002424"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OQS10_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS2293438.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="OQS10_002425"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="OQS10_002425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCS2293439.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="OQS10_002426"
     CDS             6705..6803
                     /locus_tag="OQS10_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS2293440.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="OQS10_002427"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="OQS10_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS2293441.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="OQS10_002428"
     CDS             complement(6939..7187)
                     /locus_tag="OQS10_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS2293442.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="OQS10_002429"
     CDS             complement(7335..7517)
                     /locus_tag="OQS10_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCS2293443.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OQS10_002430"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OQS10_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCS2293444.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OQS10_002431"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OQS10_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCS2293445.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OQS10_002432"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OQS10_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS2293446.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="OQS10_002433"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="OQS10_002433"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS2293447.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="OQS10_002434"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="OQS10_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS2293448.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="OQS10_002435"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="OQS10_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS2293449.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="OQS10_002436"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="OQS10_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS2293450.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OQS10_002437"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OQS10_002437"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS2293451.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OQS10_002438"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OQS10_002438"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS2293452.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OQS10_002439"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OQS10_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS2293453.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OQS10_002440"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OQS10_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS2293454.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..>20693)
                     /locus_tag="OQS10_002441"
     CDS             complement(19482..>20693)
                     /locus_tag="OQS10_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS2293455.1"
                     /translation="ASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLAQ
                     SAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDAA
                     FERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSWI
                     FTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCARQ
                     LAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFVS
                     AGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFDE
                     VQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIAR
                     AVRFLAIPSSR"
BASE COUNT         5065 a         5314 c         5039 g         5275 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc ataaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgc cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cccccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aggaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattcg
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggc
//
LOCUS       DAJVXP010000071        20232 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVXP010000071 DAJVXP010000000
VERSION     DAJVXP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.071"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            691..1461
                     /locus_tag="OQS10_002442"
     CDS             691..1461
                     /locus_tag="OQS10_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS2293456.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1452..1925
                     /locus_tag="OQS10_002443"
     CDS             1452..1925
                     /locus_tag="OQS10_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS2293457.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2033..2572
                     /gene="dnaT"
                     /locus_tag="OQS10_002444"
     CDS             2033..2572
                     /gene="dnaT"
                     /locus_tag="OQS10_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS2293458.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2575..3312
                     /gene="dnaC"
                     /locus_tag="OQS10_002445"
     CDS             2575..3312
                     /gene="dnaC"
                     /locus_tag="OQS10_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS2293459.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3361..3855
                     /locus_tag="OQS10_002446"
     CDS             3361..3855
                     /locus_tag="OQS10_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS2293460.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4109..6400
                     /gene="opgB"
                     /locus_tag="OQS10_002447"
     CDS             4109..6400
                     /gene="opgB"
                     /locus_tag="OQS10_002447"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS2293461.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6539..7561)
                     /gene="lgoD"
                     /locus_tag="OQS10_002448"
     CDS             complement(6539..7561)
                     /gene="lgoD"
                     /locus_tag="OQS10_002448"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS2293462.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7700..8614
                     /locus_tag="OQS10_002449"
     CDS             7700..8614
                     /locus_tag="OQS10_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS2293463.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8829..10180
                     /locus_tag="OQS10_002450"
                     /pseudo
     CDS             8829..10180
                     /locus_tag="OQS10_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10229..11884)
                     /gene="tsr"
                     /locus_tag="OQS10_002451"
                     /pseudo
     CDS             complement(10229..11884)
                     /gene="tsr"
                     /locus_tag="OQS10_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12003..12449)
                     /gene="hpaR"
                     /locus_tag="OQS10_002452"
     CDS             complement(12003..12449)
                     /gene="hpaR"
                     /locus_tag="OQS10_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS2293464.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12721..14010
                     /locus_tag="OQS10_002453"
     CDS             12721..14010
                     /locus_tag="OQS10_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS2293465.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14007..15473
                     /gene="hpaE"
                     /locus_tag="OQS10_002454"
     CDS             14007..15473
                     /gene="hpaE"
                     /locus_tag="OQS10_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS2293466.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15475..16326
                     /gene="hpaD"
                     /locus_tag="OQS10_002455"
     CDS             15475..16326
                     /gene="hpaD"
                     /locus_tag="OQS10_002455"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS2293467.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16336..16716
                     /locus_tag="OQS10_002456"
     CDS             16336..16716
                     /locus_tag="OQS10_002456"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS2293468.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16827..17630
                     /gene="hpaH"
                     /locus_tag="OQS10_002457"
     CDS             16827..17630
                     /gene="hpaH"
                     /locus_tag="OQS10_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS2293469.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17641..18429
                     /gene="hpaI"
                     /locus_tag="OQS10_002458"
     CDS             17641..18429
                     /gene="hpaI"
                     /locus_tag="OQS10_002458"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS2293470.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18604..19980
                     /gene="hpaX"
                     /locus_tag="OQS10_002459"
     CDS             18604..19980
                     /gene="hpaX"
                     /locus_tag="OQS10_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS2293471.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19990..>20232
                     /locus_tag="OQS10_002460"
     CDS             19990..>20232
                     /locus_tag="OQS10_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS2293472.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5430 c         5168 g         4764 t
ORIGIN      
        1 ctcttttaac cctgcttgca taacaggtat tttactgata acaattccat ttttgcagca
       61 tcctggcaac atatttcctc cacatccact gtctaaaaat catgatggtt tattcaggat
      121 attcattcct taaatgtttc cagcaaatta atagattaag cgaataaatt atccttagca
      181 ttcactttca tatcagcata ctcctgctgc acaacaactc cactgcatgt gtcagaaggt
      241 tagcatcaaa tgataacctg gtgaaatagc ctggagacat ttaaaatatc tacattaagt
      301 tgttaggaat acttaatgaa aaacagaaat cacattcatt taatttataa ttaaatgata
      361 tcccttcccg cagaatgaaa aatcacacaa ccaatattta ttcaatgaaa tctgatggtt
      421 ttaacttgtt aacattaaaa aacgtgcccg gttatctatt atcctgattc gtaaaggcca
      481 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      541 ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct
      601 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      661 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaaca
      721 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      781 gatgagcttt cctcacgact gggtcgggcg ttggggatgg acagcgtcga aagttctatc
      841 tcttcgaacg ccatagtgct gacaaccatt aaagatgggc aatgcctgac atcgacacgt
      901 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      961 ctggcggagc accatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag
     1021 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
     1081 tgcaaactca ataaaggtgg ctgggatggt gccgtcatca ccttctttgc cagtacggcc
     1141 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccgcagat caacttttgc
     1201 ctcaccgctt tcgccgccac caccatttcc ggattgcttt tgcaacttcc cactttcagc
     1261 aatacaccca ctatcgcgat ggccgccagc gttctgctgc tggtgccggg atttccgttg
     1321 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
     1381 atcgccagtc tgctgacact ggctacctgc gtcggcgtgg tgatggcact gacgatttgg
     1441 gggctacgcg gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg
     1501 ccgccattcc agcggtcggc tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct
     1561 ggtgtgcgct gcttggcgcg attggtcatg gttcacgaat gatcttaatg accagcgggc
     1621 tgaatattga gtggtcaacc tttatggcct ccatgctggt cggcaccatt ggtattcaat
     1681 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1741 tcccgggcat atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt
     1801 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1861 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1921 tataaaattg cctcgcttaa agcacacgga tgagagacag cctcctctcc tccgtgtgtt
     1981 actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc
     2041 cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt
     2101 tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta
     2161 tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc
     2221 gggaagcgat gtcgctctgg acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc
     2281 tgtacccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg attttatccg
     2341 cctggcggcg ctatggggtg tggcactaag agagccggtg accaccgaag aactggcctc
     2401 attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa
     2461 actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt
     2521 gaatacggtc agcgaacctg acagtcaaat tccaccagga ttcagagggt aacgatgaaa
     2581 aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca
     2641 ttcaaaacgg gtgaagaact actggcctgg cagaaagaac aaggagcgat ccgctccgcc
     2701 gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt
     2761 cggccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg
     2821 aatgcgttaa gcaaagcgcg ccagtacgtc gaagagtttg acggcaacat cgccagcttc
     2881 atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag
     2941 ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg
     3001 aaagatacct tcaggaatag cggtaccagt gaagaacaac tgcttaacga tctgagcaac
     3061 gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg
     3121 atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc
     3181 aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc
     3241 ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg ggtaacaggt
     3301 aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg
     3361 atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc
     3421 accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc
     3481 aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac taacttgctt
     3541 agcagcggga accaggcctt aagcgcagat aacatgaaca acgccgcagg cattctgcaa
     3601 tactgcgcga agcaaaagct ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg
     3661 ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggatggc
     3721 attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa catcggaacg
     3781 actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg
     3841 aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc
     3901 tctcctgccc ttttccctgc tcctggacgg ttttccccct gttacacggg aagcgaccgc
     3961 gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga
     4021 attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta
     4081 aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg ccctttttct
     4141 cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac
     4201 attaacggtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt
     4261 tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc
     4321 tggcgtcagc aaatacatcc tgccgggtat cggcattgtg ctggggctga cagcggtgtt
     4381 cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag
     4441 cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat
     4501 aacggaactg gtgaaatccc agtcacgcga tggcgacccg gactttgcgg cttattataa
     4561 agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct acggcgaaag
     4621 tctcgagcgg acctattttg ataacgaagc tttcccggat ctcacgcctg aactgggcgc
     4681 gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccaggaa cggattacac
     4741 gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa
     4801 cgcctccgcc tctgtctcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa
     4861 aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga
     4921 tgtgttcctg aagtcgcatg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt
     4981 ggtggccgac ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga
     5041 agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct
     5101 gacagtcgat acccatcacc cggatggttt tatctcgcgt acctgtaacc gcaaaaaata
     5161 tgattttgac ggtaagccga accagtcatt cagcgcggta agttgcagcc aggagaatat
     5221 cgcggcgttt atcaacaaaa tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt
     5281 ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg
     5341 caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc tggcagtgaa
     5401 gcgtaacacg atggataacg gcgcgacggt gctggacatt ctcggtggcg ataactatct
     5461 cggacttggt cgtagcagtt tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa
     5521 agagaaaaca ttggcgtgga agccggatat catccgcctg tggaaattcc ctaaagagat
     5581 gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg
     5641 tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata
     5701 ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt
     5761 tgaccgttgc tataagatgg cacaactctg ggctccggaa ctggcactct ccaccgactg
     5821 gtgtgtctcg caagggcagc ttggcggtca gcaaattgtt cagcatgttg acaaaacaac
     5881 atggcagggc aaaacggcat ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa
     5941 tgtcgatacg ctgaaaattg ttgataacga tattcgctac aaagccgaca gtttcatctt
     6001 caacgtcgcc ggtgcgccag aagaggtgaa acagtttagt gggatttccc gtccagagtc
     6061 gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc
     6121 gctgccaaag aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg
     6181 tcctattccg gtacgcgtag gcaatgaaga acaaaccctt gtgctgggca atgaagtgac
     6241 caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc
     6301 ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat
     6361 cggcatggtg gaaattaaag tggtggaacg tgaaggataa ttcctgactg atgtgtaggt
     6421 cgcataaggt gcgacctaca caccttctgc ggcctacaaa acatcattga gaaacagaaa
     6481 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc
     6541 agaaagtaat tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt
     6601 ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcagcagtg atttttcctt
     6661 ccgccatcag tcgaccgact ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca
     6721 tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct ttaaacaggc
     6781 cgacaaatac caccgtgccg ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt
     6841 gattacctgt cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgtagct
     6901 gcgcgtcaaa atcttcggct gacgggtcca gtaaaggtaa ttccagacgc gttgccacat
     6961 gttcacggcg cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga
     7021 ttgccgccgc gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag
     7081 caatggctgc gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgctgccg
     7141 cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac
     7201 taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc
     7261 cgcttttaca cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac
     7321 catttttaag atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc
     7381 ctaaaacacg tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac
     7441 cgcaaatccc gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct
     7501 cgcgttgttt ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca
     7561 tgattcggct ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg
     7621 aacggaaaat ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg
     7681 gactcacaag gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca
     7741 ggtcattgat gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct
     7801 ggcggaaatg tacaacatca gccgcaccac agtgcgtcat attctcagcc acttacgcga
     7861 atgcggcgtc ttgacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga
     7921 tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc
     7981 ttttttcacg atgataaacc agcgccagtt acgcccaggg gaaacgtttt ctgaactgca
     8041 actggctcgg gccgcgggtg tcagcccggt tgtagtaaga gaatatcttt taaaattcgg
     8101 ccgttataat cttattcaca gcgaaaagcg cggccagtgg agcatgaagc aattcgatca
     8161 atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca
     8221 ctttctcaac ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg
     8281 tcatcggcta ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag
     8341 agattttcac tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga
     8401 gatcatctca gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg
     8461 caatatcatt gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga
     8521 ccttgatgcc acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg
     8581 atccattaat gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt
     8641 agcgggtgca cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca
     8701 caatcagtcg actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc
     8761 cgttaatgtc aaccttgcgt aattcacaat tacgtaaaca attaaaatta taaatacagg
     8821 agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt
     8881 cgtcggcaga tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac
     8941 gcattcaaac caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc
     9001 gcagttcgct gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg
     9061 aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg
     9121 gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt
     9181 cactgttcca ggcaatgtct ggcatggtgc acaactttac gcagttcgtg ttggtgcgta
     9241 tcggtatggg gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact
     9301 ggttcaacat caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg
     9361 gcgttgccgt aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgcggga
     9421 tgtttattac cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc
     9481 gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg
     9541 tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcctgttc cgtaaccgta
     9601 caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg
     9661 cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga
     9721 tggcggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg
     9781 actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg
     9841 ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca agcgacaaca tccatgacag
     9901 cggttctgct gattggcatg gcactgttct gtattcaaca tcctgctggg gcttgatcca
     9961 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
    10021 catctgcgcc tcttttgcgc cgatcattac tggttttatt gttgatacca cccactcatt
    10081 ccgtctggca ctaatcatct gcggttgcgt caccgcagcg ggggcactgg cgtacatctt
    10141 cctggttcgt cagccgatca acgacccacg taaagattaa tctccttatg cccgataaca
    10201 ttttgcttat cgggcatttt catggcgatt aaaatgtttc ccagttctcc tcgctatctg
    10261 ccacggccat tttacgcggc gcagctggcg tcacggtttt taccacagcc gatgtttcac
    10321 gctgctgttg ctgaatccgg aacactgcca ctgcttcggt caggcgactg gcctgctctt
    10381 ccagcgcggc ggcggcagcg gcagactctt ccaccagcgc ggcgttctgt tgagttaccc
    10441 ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag
    10501 cagaagcaat ttcgcccata atgtccgtca cgcgggtcac ggcgctgaca atctccgcca
    10561 ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt
    10621 caatcaggct tttaatttca cgagccgcct gggcgctgcg ctgggccaga ttacgcactt
    10681 ctcccgcgac caccgcaaaa ccgcgccctt gctcacccgc acgcgcagcc tcaaccgccg
    10741 cgttcaaagc cagaatattg gtctggaagg caatgccgtc aattacgctg ataatatcgg
    10801 cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta tctaccactt
    10861 tgccgccgcg ttgcgccgtt tcagaagcac ttaacgccag atggctggcc tggcgcgcat
    10921 tttcggcgtt ctgcttcacc gttgcggtca gttactccat gctggctgcc gtctcttcca
    10981 gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg gcgatttcgc
    11041 tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct
    11101 ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct
    11161 caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca
    11221 ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc gccagtacga
    11281 cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga
    11341 cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac
    11401 cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct gcacctaaca
    11461 gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgccg
    11521 ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt
    11581 ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac
    11641 caatattgtt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct
    11701 gcaacaacgc gacccagctg ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa
    11761 cagtgaaatt ttctttgtca ttctttaagg cattaaagaa cagaccgcct gatgtcagtt
    11821 gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta
    11881 acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag caaaaacttt
    11941 atgaaaaggt gtaacccacc atccatggaa aaaccaggcg ctggttatca gatactaaaa
    12001 agttattcat tatcgcccgg gatggcttct tgtcgggaat tgcccagagc aataaattcc
    12061 tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc aatctgccga
    12121 taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc tttggttagc
    12181 gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa accgtcgcgt
    12241 tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga
    12301 tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca ctgttgctca
    12361 gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc tcgcgcctgg
    12421 agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc aaaatcatta
    12481 atatagaaac agttaatcgt cacgttaccg ggactgcgct gcccctgcaa ttcctgattt
    12541 tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt atctttaaat
    12601 gtaaatagtt tgttaattag atcacattta catcacttac ttttgccaaa ctacgcaatc
    12661 gggaataaaa gcaatcatta acatattaat gattaagatg atcgaatccg aggagtggta
    12721 atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga tgcatggcag
    12781 gaagcgttcc agcaatcccc ctacaaagcc ccgcctaaaa ctgcggtctg gtttattaaa
    12841 ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg tgaaaaggta
    12901 ctgagcggtg cgactgttgc gctgattgtg ggaaaaacgg cgacgaaagt acgtgaagaa
    12961 gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct gccggaagag
    13021 agcttttacc gcccggcaat caaagcaaaa tgtcgtgatg gattctgccc cattggcgaa
    13081 accgtggctc tcagcaatgt cgataatctg accatctata ccgagatcaa cgggcgtcct
    13141 gccgatcact ggaataccgc cgatttacaa cgtaacgccg cgcagttgct gagcgccctg
    13201 agcgaatttg ccacgctgaa tccaggcgac gccattctgc tcggcacgcc acaggcgcgc
    13261 gtggaaatac agccaggcga tcgcgttcgt gttctcgcag aaggtttccc gccgctggaa
    13321 aatccggtag tggacgaacg tgaagtgacc acgcacaaga gcttcccaac gcagccacac
    13381 ccgcacggca cgctgtttgc cctcggcctg aactacgccg accacgccag cgaactggaa
    13441 tttaagccac cggaagaacc gctggtgttc ctgaaagcgc caaataccct cactggcgat
    13501 aaccagacct ccgtgcgtcc aaacaatatt gaatacatgc actatgaagc ggagctggtg
    13561 gtagttattg gcaaacaggc gcgtaacgtc agcgaagccg atgccatgga ttatgtcgcg
    13621 ggctacaccg tgtgtaacga ctacgccatt cgtgactatc tggaaaacta ctaccgccct
    13681 aacctgcggg taaaaagccg cgacggactg acgccgatgc tttcaaccat cgtgccgaaa
    13741 gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg cgagttacgc
    13801 cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc ctacttaagt
    13861 gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacacccaa aggcttatct
    13921 gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg cctggtgaac
    13981 cgaattgtga gtgaggatac agcgaaatga aaaaagtaaa tcattggatc aacggcaaaa
    14041 atgttgcagg taacgactac ttccagacca ccaatccggc aacgggtgaa gtgctggcgg
    14101 atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg aaagaggcgt
    14161 tcccgaaatg ggccaatctg ccgatgaaag agcgtgcgcg cctgatgcgc cgtctgggcg
    14221 atctgatcga ccagaacgtg ccagagatcg ccgcgatgga aaccgcggac accggcctgc
    14281 cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc ccacaacttt gaatttttcg
    14341 cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag atgctcaact
    14401 acacgctggt gcagccggtg ggcgtttgtg cgctggtatc gccgtggaac gtaccgttta
    14461 tgaccgccac atggaaggtc gcgccgtgtc tggcgctggg caataccgcg gtactgaaaa
    14521 tgtcggaact ctccccgctg accgctgaca gcctgggtga gctggcgctg gaagccgata
    14581 ttccggcagg cgtgctgaac gtggtacagg gctacggcgc aaccgcaggg gatgcgctgg
    14641 ttcgtcatca tgacgtacgt gccgtgtcgt tcaccggcgg tacggccacc gggcgcaaca
    14701 tcatgaaaaa cgccgggctg aaaaaatact ccatggaact gggcggtaaa tcgccggtgc
    14761 tgatttttga agatgccgat attgaacgcg cgctggacgc cgccctgttc accatcttct
    14821 cgatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcaa agcatctacc
    14881 cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc gatccgaacg
    14941 atccgaatac ccaggttggc gcgcttatta gccagcagca ctgggaaaaa gtctccggct
    15001 atatccgtct cggcattgaa gaaggcgcaa ccctgctggc gggcggcccg gataaaccgt
    15061 ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaaccgtg ctggcagacg
    15121 ttgataaccg tatgcgagtc gcccaggaag agattttcgg gccggtcgcc tgcctgctgc
    15181 cgtttaaaga cgaagccgaa ggcttacgtc tggcaaacga cgtggagtac ggcctcgcgt
    15241 cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc attgaagctg
    15301 gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg ccagccattt ggcggcgtaa
    15361 aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc gcggaaatga
    15421 agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc tgatatgggt
    15481 aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatcactc tgaactgcca
    15541 gggaaaaacc acggttgccg ccagggcgcg atcgacgggc ataaagagat cagcaagcgt
    15601 tgccgggaaa tgggcgtcga taccattatc gttttcgata cccactggct ggtcaacagt
    15661 gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa cgagctgccg
    15721 cattttatcc gcgatatgac ctacaactac gagggcaacc cggagttggg gcagcttatt
    15781 gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc acaacattcc cagcctgaaa
    15841 ctggaatacg gcacgctggt gccgatgcgc tacatgaatg aagacaagca cttcaaagtg
    15901 gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa gctgggcgaa
    15961 gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc cagcggttcg
    16021 ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag ctacacccgc
    16081 gagttcgacc gccagatgga cgagcgcgtg gtgaagttgt ggcgcgaagg ccagttcaaa
    16141 gagttctgca atatgctgcc ggagtacgcc gactactgct acggcgaagg caatatgcac
    16201 gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa ggtggagttt
    16261 atcaccgagc tattcccaag ctctggcacc ggtcaggtta acgctgtttt cccgcttccc
    16321 gcgtaaggac gttttatgcc gcactttatc gttaaatgca gtgacaacat ccgcgaagaa
    16381 gccgacctgc cggggttgtt cgccaaagtg aatccgacgc tggcagccac gggtattttt
    16441 ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata cctggcagat ggccgacggg
    16501 cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg gcgcaggtcg cagcctggaa
    16561 agccgccagc aggcgggtga aatgctgttt gaactgatta aaacgcactt cgccgccctg
    16621 atggagagcc gcctgctggc gttgtcgttt gagattgaag agctgcatcc gacgctgaat
    16681 tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg cagattgccc ggtggcgctg
    16741 cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag gccggataag gcgcagccgc
    16801 atccggcaat gccacaggat atcgctatgt tcgataaaca cacccacacc ctgatcgccc
    16861 agcgtctgga tcaggcagaa aaacagcgcg aacagatccg cgcgatctcg ctggattacc
    16921 cggagatcac catcgaagac gcttacgcgg tgcagcgtga atgggttcgc ctgaaaatcg
    16981 ccgaaggtcg cacgctgaaa ggccacaaaa tcggcctgac ctcgaaagcg atgcaggcaa
    17041 gctcgcagat cagcgaaccg gattacggcg cgctgctgga tgacatgttc ttccacgatg
    17101 gcagcgatat cccgaccgat cgctttatcg tgccgcgcat tgaagtggag ctggctttcg
    17161 tgctggcaaa accgctgcgt gggccaaact gcacgctgtt cgacgtctac aacgccacgg
    17221 actacgtgat cccggcgctg gagctgatcg acgctcgctg ccacaacatc gatccggaaa
    17281 cccagcgccc gcgcaaagtg ttcgacacca tttctgataa cgccgccaac gccggggtga
    17341 tcctcggcgg tcgtccgatc aaaccagacg aactggatct gcgttggatc tccgccctga
    17401 tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc tggcgtgctg aatcatccgg
    17461 caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta tgacgtacaa ctggaagccg
    17521 ggcaaatcat tctcggtggt tcgttcaccc gcccggttcc ggcgcgtaag ggcgacacct
    17581 tccacgtcga ttacggcaac atgggttcca ttagctgccg ctttgtttaa ggagagaacg
    17641 atggaaaaca gctttaaagc ggcgctgaaa gcaggccgtc cgcagatcgg attatggctg
    17701 gggctgagta gcagctacag cgcagagtta ctggccggag caggattcga ctggttgttg
    17761 atcgacggtg agcacacgcc gaataacgtg caaaccgtgc tcacccagct acaggcgatt
    17821 gcgccctacc ccagccagcc ggtagtacgt ccgtcgtgga acgatccggt gcaaatcaaa
    17881 caactgctgg acgtcggcac acaaaccttg ctggtgccga tggtacaaaa cgctgacgaa
    17941 gcccgtgaag cggtacgcgc cacccgttat ccccccgccg gtattcgcgg tgtgggcagt
    18001 gcgctggccc gcgcctcgcg ctggaatcgt attcctgatt acctgcaaaa agccaacgat
    18061 caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa tgaagaactt accgcagatt
    18121 ctggacgtgg aaggcgtcga cggcgtgttt atcggcccgg cggatctgag cgccgatatg
    18181 ggttatgccg gtaatccgca gcacccggaa gtacaggccg ccattgagca ggcgatcgtg
    18241 cagatccgcg aagcgggcaa agcgccgggg atcctgatcg ccaatgagca actggcaaaa
    18301 cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg ttgacaccac cctgctcgcc
    18361 cgcgccgccg aagcgctggc agcacggttt ggtgcgcagg ccaccgccgt gaagcccggc
    18421 gtgtattaat gccggatgcg ctgcgcttat ccggcctaca ctcgcaccaa accgtaggcc
    18481 ggataaggcg tttacgccgc atccggcaaa aagccgtacc aaatcgcgga ccggataagg
    18541 cgttacgccg catccgccat aaaccttgtc gtaccctaca aaaatcccat tagaggaaga
    18601 aaaatgagcg acacctcacc tgccataccg gagagtatcg atccggcgaa tcagcataaa
    18661 gcgctgactg ccggacaaca ggcggttatt aagaagctat ttcgccgcct gagcgtcttt
    18721 ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca acatcggctt tgccggactc
    18781 acgatgggac gcgacctcgg tctgagcgcc accatgtttg gcctcgctac caccctgttc
    18841 tatgccgctt atgtcatctt cggcattccc agcaacatta tgctgagtat tgtcggtgcg
    18901 cggcgctgga tcgccaccat catggtgctc tggggcatcg cctctactgc caccatgttt
    18961 gccactggcc ccaccagctt gtacgtactg cgtatactgg ttggcattac cgaagccggc
    19021 tttctgcctg gtattctgct gtatttaacc ttctggtttc cggcctattt ccgcgcccgt
    19081 gccaacgcct tgtttatggt agcaatgccg gtaacgacag cgttgggctc gatcgtttcc
    19141 ggctacattt tgtcgctgga tggcgtcatg gcattaaaag gctggcagtg gctgtttttg
    19201 ctggaaggct tcccgtcggt attactcggc gtcatggtgt ggttctggct tgatgactca
    19261 ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat gcctgcaaga gatgatggat
    19321 aacgatcgtc tgacgctggt tcagccagag ggagccatca gccaccacgc catgcaacaa
    19381 cgcagtatgt ggcgggagat cttcactccg gtggtgatga tgtataccct ggcgtatttc
    19441 tgcctgacca acacacttag tgcgatcagc atctggacac cgcagatcct gcaaagtttt
    19501 aatcagggca gcagtaatat caccatcggc atgctggccg ccgtaccgca gatttgtacc
    19561 attctcggga tggtctactg gagccgtcac tcagatcgcc gccaggaacg aaggcatcac
    19621 accgcccttc cttatttgtt cgctgccgca ggttggttac tggcttcggc aactgatcac
    19681 aacatgatcc agatgctggg gatcattatg gcttcgaccg gatcattcag cgcaatggcg
    19741 attttctgga caacaccgga tcagtccatc agcctgcggg cacgagcgat cggtattgcg
    19801 gtgatcaacg ccactggcaa cattggttca gcgttaagtc cgtttatgat cggctggttg
    19861 aaagatttga ccggcagctt taacagtgga ttgtggtttg ttgccgcgct gctggtgatt
    19921 ggtgcgggga ttatctgggc gattccaatg cagtcctccc gtccgcgagc gaccctgtaa
    19981 ggaacgacga tgtgtgaccg tcagattgcc aatattgata tcagcaaaga gtacgatgaa
    20041 agcctgggca cggacgatgt gcattatcag tcgttcgccc gcatggcggc attttttggc
    20101 cgccatatgc tgccacatcg ccacgaacag tactttcaga tgcatttcct caatagcgga
    20161 cagattgagc tacagcttga cgatcatcgc tactcggtgg aagcgcccct gtttgtcctg
    20221 acgccgccgt ca
//
LOCUS       DAJVXP010000072        20147 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVXP010000072 DAJVXP010000000
VERSION     DAJVXP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.072"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="OQS10_002461"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="OQS10_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCS2293473.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="OQS10_002462"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="OQS10_002462"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS2293474.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="OQS10_002463"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="OQS10_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS2293475.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /locus_tag="OQS10_002464"
     CDS             3608..5233
                     /locus_tag="OQS10_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS2293476.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="OQS10_002465"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="OQS10_002465"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS2293477.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="OQS10_002466"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="OQS10_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="OQS10_002467"
     CDS             complement(7691..8902)
                     /locus_tag="OQS10_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS2293478.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="OQS10_002468"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="OQS10_002468"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS2293479.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="OQS10_002469"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="OQS10_002469"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS2293480.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="OQS10_002470"
     CDS             11880..13658
                     /locus_tag="OQS10_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCS2293481.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="OQS10_002471"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="OQS10_002471"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS2293482.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="OQS10_002472"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="OQS10_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS2293483.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="OQS10_002473"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="OQS10_002473"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS2293484.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /locus_tag="OQS10_002474"
     CDS             17775..18530
                     /locus_tag="OQS10_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS2293485.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="OQS10_002475"
     tRNA            18609..18678
                     /locus_tag="OQS10_002475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18871..>20147
                     /locus_tag="OQS10_002476"
     CDS             18871..>20147
                     /locus_tag="OQS10_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293486.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4980 a         4765 c         5180 g         5222 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgagca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcatag ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAJVXP010000073        20077 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVXP010000073 DAJVXP010000000
VERSION     DAJVXP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.073"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            59..568
                     /gene="radC"
                     /locus_tag="OQS10_002477"
                     /pseudo
     CDS             59..568
                     /gene="radC"
                     /locus_tag="OQS10_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="OQS10_002478"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="OQS10_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS2293487.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="OQS10_002479"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="OQS10_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS2293488.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="OQS10_002480"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="OQS10_002480"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS2293489.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="OQS10_002481"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="OQS10_002481"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS2293490.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="OQS10_002482"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="OQS10_002482"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS2293491.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="OQS10_002483"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="OQS10_002483"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS2293492.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="OQS10_002484"
     CDS             5387..6511
                     /locus_tag="OQS10_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS2293493.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="OQS10_002485"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="OQS10_002485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS2293494.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..8333
                     /gene="waaO"
                     /locus_tag="OQS10_002486"
                     /gene_synonym="rfaI"
     CDS             7317..8333
                     /gene="waaO"
                     /locus_tag="OQS10_002486"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS2293495.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8350..9345
                     /locus_tag="OQS10_002487"
     CDS             8350..9345
                     /locus_tag="OQS10_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS2293496.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9355..10047
                     /gene="rfaY"
                     /locus_tag="OQS10_002488"
     CDS             9355..10047
                     /gene="rfaY"
                     /locus_tag="OQS10_002488"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS2293497.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10073..11098
                     /locus_tag="OQS10_002489"
     CDS             10073..11098
                     /locus_tag="OQS10_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS2293498.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11183..12166
                     /locus_tag="OQS10_002490"
     CDS             11183..12166
                     /locus_tag="OQS10_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS2293499.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12212..13465
                     /locus_tag="OQS10_002491"
     CDS             12212..13465
                     /locus_tag="OQS10_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS2293500.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="OQS10_002492"
     CDS             complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="OQS10_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS2293501.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="OQS10_002493"
     CDS             complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="OQS10_002493"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS2293502.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="OQS10_002494"
     CDS             complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="OQS10_002494"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS2293503.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16712..17908
                     /gene="kbl"
                     /locus_tag="OQS10_002495"
     CDS             16712..17908
                     /gene="kbl"
                     /locus_tag="OQS10_002495"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS2293504.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17918..18943
                     /gene="tdh"
                     /locus_tag="OQS10_002496"
     CDS             17918..18943
                     /gene="tdh"
                     /locus_tag="OQS10_002496"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS2293505.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19182..>20077
                     /gene="waaH"
                     /locus_tag="OQS10_002497"
     CDS             19182..>20077
                     /gene="waaH"
                     /locus_tag="OQS10_002497"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCS2293506.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5422 a         4377 c         4738 g         5540 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactatgct cgttattgcg ccaagcataa ttttaaacaa aatgaaccaa
     8281 ttaacgggat aatgaattat atttattatt tttatttaaa gataataaaa tgatattgag
     8341 aaaagttaaa tgaatgaatt tataaaagaa cggttttcgt atttagcaga taataaaaaa
     8401 gaaaacgccc cagaactaaa tgtttcctac ggtatcgata agaatttttt gtatggtgct
     8461 ggcgtttcaa tttcttccgt tttgattaat aattcagata ttaattttgt ctttcatgtt
     8521 ttcactgatt atgtggatga tgattattta aagtcattta atgaaacagc aaaacaattt
     8581 aatacctcaa ttattgtata tttaattgac ccaaaatact ttgctgatct gccgacgtca
     8641 cagttttggt cgtacgcgac atacttcagg gtattgtcct ttgaatatct gagtgaaagt
     8701 atttccacac tgctgtatct ggatgccgat gtcgtttgta aaggaagtct gaagcctctc
     8761 acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatgt tagtactcag
     8821 gcggcatgtg caaaacgcct caacattccc gagatgaatg gacgttattt caatgcaggc
     8881 gttatctatg tcaatattaa aaaatggcat gaagcaaatt tgacaccgta tttactcacg
     8941 cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat
     9001 atcgcattta atatgaataa tatctacctc gcgaaggatt ttgatactat ttataccctg
     9061 aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca
     9121 gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg ttatccgtct
     9181 gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag
     9241 gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca tggtgagtat
     9301 attaaaggca taacttcatt aattaagtac aagcttaaga aataaattac tcttatgatt
     9361 acaagtatac gctatcgcgg cttctcattt tattacaaag ataatgataa taaatataaa
     9421 gaaatctttg atgaaatact ggcctataat tttaaaacag taaaagtatt acgtaatatt
     9481 aatgatacca aagtatcatt gattgatacg aaatattgtc gctatgtttt taaagtcttt
     9541 gccccgaaaa cgaaaaggaa tgaacgtttt ttaaaatctt ttgtaaaagg tgactactat
     9601 cagaatttga tcgttgaaac cgatcgtgtc agaagtgcag gtcttacttt tcctaatgat
     9661 ttttattttc tggctgagcg taaaattttc aactatgcca gtgtttttat catgcttatc
     9721 gagtatgtcg agggcgtaga gctcaatgat atgccgatca ttccagaaga tgttaaagca
     9781 gaaattaagg cttcaatgga aaaattgcat gccttaaata tgctatcggg cgacccacac
     9841 cggggtaact ttatcgttag taaagatggt gtcaggataa ttgacctatc cggtaaaagc
     9901 tgtacggcag agcgtaaagc tcgtgatcgt ctggccatgg aacgtcacct gggtattgcc
     9961 aacgaaataa aagattatgg ctactattca gtaatctaca gaaccaaact acgcaaattt
    10021 attaaaaaat taaaaggcaa agcgtaaacc acacagtcaa aacggaacca acatggattt
    10081 attagctgag agtattactg aagtcgctgt ctctggggaa attgctaaca ccgatcgtgt
    10141 gttaaatatc gcttacggta ttgaccgtaa ctttttattt ggtgcggcag tatctatgca
    10201 atcagttgtt atgcataatc cggaccttgc ggttaagttt catctcttta ccgactacat
    10261 tgatgaagat tatctacaac gtgttaatgc ttttaccagc aaaaatgcta acgttgaagt
    10321 aagaatttat aaagtctcca gtgcctttat tgatatcttc cccagcctga aacagtggtc
    10381 ttatgcaaca ttcttccgtt tagttgcgtt ccagtatctg agtgaaacta ttgaaaatct
    10441 gttatatatc gatgctgatg tcatctgtaa aggctcatta gctggattgc ttgatattaa
    10501 ttttgatggg gataagtttg cagctgttat taaagatgtg ccttttatgc aggaaaaacc
    10561 agcgaagcgt ctggctatag agggacttcc agggaattat ttcaacgccg gtgtagtata
    10621 tctgcagctt gaagcatggg cgaaaaatga ttttatgaat aaagccattg ctatgctggc
    10681 aagtgacccg cagcacacga agtataaatg ccttgatcag gatattttaa atattctgtt
    10741 ctttggtcat tgtattttta ttagcggcga ttatgattgc ttttatggca tcgattatga
    10801 gttaaaaaat aaaagcgatg aagattataa aaagaccatt accgatgata ctaagctgat
    10861 tcattatgtt ggcgtaacga agccctggaa cgactggacg aattatccct gccagaagta
    10921 ttttaatgag gcttatcagg cttcttgctg gaatgatgtg gcgtttattc cagccacgaa
    10981 tgaaaagcag tatcaagtga aatatcaaca tgcaaagaaa aatggtgata cgtttaacgc
    11041 ttttatttac tttattaaat ttaaattaaa taagtataaa agaaaactat ttggataata
    11101 ataaagatca tggatgtctg agttacaata ataattaagg atgagtacta tcatatttta
    11161 ttagttcagg caagagaaca cgatgagcaa tgattaccct ttagtatcca taataatacc
    11221 gacgtataat tcatctgatt acattactga aactctaacg aaattagaaa aacaaactta
    11281 cccaaatttt gaaattgtta ttgttaatga tggttctaaa gataacacat caaacgtttt
    11341 gagagagtat gggttaaccc actctcgatt aattattatc aataaagaaa atggcggtgt
    11401 ttcgtctgcc aggaatacag gtatccgcaa ggcgcaagga cagtttatat gttttatgga
    11461 tgatgatgat gagatagatc ctaactatct gctgaagatg tataccagac aacatgagac
    11521 gggaggggat gcgatttatt gtgggcttta tggccattat ataaaagatg gtgttactta
    11581 ctcacctata aatacagagt ttaatgaagg atctttactt ttcgactttt tttataaaaa
    11641 ggttagattc catatagggt gcttgtttat aagaaaacaa cttctggaag agaataatct
    11701 tttttttgat gaagatttac gactaggaga agatctggat tttatctatc gactgctaat
    11761 tacatgcgat atgtatgcgg ttccatatta tatgtataag cataactata gagaaaattc
    11821 cttaatgaac tcatgtagaa ccatcactca ttatcgacat gagtcatttg cgcacgaaag
    11881 aatctactct tctgtgatgc agttatacaa aggtaacagg aaagaagaaa ttcatacatt
    11941 attgagtcaa aatagagctt atcataaaac tcgttattta tggaatgttc tacttaatgg
    12001 tgattttgaa ctattaaatc aattagttga aagcaatgaa aaagaattga atgattgtaa
    12061 tctttctggc aagagagata aaagacgagc aaaaatatta gcatcaaaaa attatattct
    12121 ctggaggatg gtaagactgg taaataggaa aaagaataaa cgttagatct tgcattaaat
    12181 aatccgtcct cgtagcatag gttgaaacta tatgtcattt tgttggaatg aaattaattc
    12241 tggtataaag tctttaattc tcatattatg tattttttct ttgatgactt tgtctttatg
    12301 ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt atatctgcat tgtattttct
    12361 aataacacca aaaaaaacaa taactaacaa tcctactttg ttaattttca tctcattatg
    12421 tcttttggga atcataaata tcatctggta ttcacattat aaagtttcag gctctgttta
    12481 taccaatgca tatcgtggcc caatggaaac tgggaaaatt gccttgtgta gtgcttttat
    12541 tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg ggaaactaat
    12601 tctgttcgca tccctggcaa cgcagttact tttttttgcg catgccatgt ggcaacattt
    12661 ctatttaaac gttgaccgtg ttgcattatc agcttcccac gctacaacag caggctacat
    12721 catccttttc ccttctttac tggcatcaat tctcatttta aaatccgact ttagacataa
    12781 aacaacatta tatacaatta acttcatgct tagcttatgt gctgtcatag taactgaaac
    12841 gcgtgcagcc atattagtgt ttccattctt tgcgttacta ttaatcgtaa tggatagtta
    12901 tattaataag cgaattaatt ataagttata ttgttttatt gcgattgcat tattagcagg
    12961 tgtattttct tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt
    13021 taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt
    13081 tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca
    13141 tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt
    13201 gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat
    13261 tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt
    13321 aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc
    13381 ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc
    13441 agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat
    13501 tttatttcaa aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa
    13561 tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa
    13621 ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga
    13681 tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa
    13741 atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa
    13801 cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg
    13861 gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt
    13921 ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat
    13981 ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt
    14041 tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt
    14101 ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc
    14161 agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca
    14221 gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt
    14281 tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag
    14341 gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca
    14401 cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat
    14461 ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct
    14521 cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc
    14581 tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc
    14641 ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca
    14701 gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga
    14761 caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca
    14821 ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt
    14881 catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg
    14941 ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc
    15001 aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt
    15061 caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac
    15121 gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt
    15181 ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg
    15241 tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga
    15301 cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga
    15361 tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg
    15421 ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg
    15481 cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga
    15541 tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg
    15601 ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca
    15661 gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt
    15721 cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg
    15781 cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca
    15841 catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca
    15901 gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc
    15961 tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg
    16021 agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt
    16081 agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt
    16141 aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca
    16201 gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg
    16261 aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa
    16321 tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca
    16381 caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat
    16441 tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa
    16501 ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc
    16561 actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc
    16621 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
    16681 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
    16741 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
    16801 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
    16861 taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga
    16921 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
    16981 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
    17041 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
    17101 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
    17161 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
    17221 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat
    17281 tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga
    17341 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
    17401 tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc
    17461 tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc
    17521 gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga
    17581 gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg
    17641 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt
    17701 catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
    17761 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
    17821 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
    17881 tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa
    17941 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
    18001 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
    18061 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga
    18121 agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga
    18181 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
    18241 caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc
    18301 ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga
    18361 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct
    18421 ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc
    18481 acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg
    18541 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
    18601 catggccgaa ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac
    18661 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
    18721 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
    18781 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
    18841 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
    18901 gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt
    18961 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
    19021 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
    19081 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
    19141 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
    19201 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
    19261 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
    19321 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
    19381 atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca
    19441 aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga
    19501 tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc
    19561 gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat
    19621 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
    19681 ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat
    19741 tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat
    19801 ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata
    19861 gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag
    19921 agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa
    19981 taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc
    20041 gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAJVXP010000074        19926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVXP010000074 DAJVXP010000000
VERSION     DAJVXP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.074"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1306)
                     /locus_tag="OQS10_002498"
     CDS             complement(<1..1306)
                     /locus_tag="OQS10_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HCS2293507.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLA"
     gene            complement(1303..1616)
                     /locus_tag="OQS10_002499"
                     /pseudo
     CDS             complement(1303..1616)
                     /locus_tag="OQS10_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(1816..3774)
                     /gene="acs"
                     /locus_tag="OQS10_002500"
     CDS             complement(1816..3774)
                     /gene="acs"
                     /locus_tag="OQS10_002500"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS2293508.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4168..5604
                     /gene="nrfA"
                     /locus_tag="OQS10_002501"
     CDS             4168..5604
                     /gene="nrfA"
                     /locus_tag="OQS10_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS2293509.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5649..6215
                     /gene="nrfB"
                     /locus_tag="OQS10_002502"
     CDS             5649..6215
                     /gene="nrfB"
                     /locus_tag="OQS10_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS2293510.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6212..6883
                     /gene="nrfC"
                     /locus_tag="OQS10_002503"
     CDS             6212..6883
                     /gene="nrfC"
                     /locus_tag="OQS10_002503"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS2293511.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            6880..7836
                     /gene="nrfD"
                     /locus_tag="OQS10_002504"
     CDS             6880..7836
                     /gene="nrfD"
                     /locus_tag="OQS10_002504"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS2293512.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            7856..9574
                     /locus_tag="OQS10_002505"
     CDS             7856..9574
                     /locus_tag="OQS10_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS2293513.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9567..9950
                     /gene="nrfF"
                     /locus_tag="OQS10_002506"
     CDS             9567..9950
                     /gene="nrfF"
                     /locus_tag="OQS10_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS2293514.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            9947..10543
                     /gene="nrfG"
                     /locus_tag="OQS10_002507"
     CDS             9947..10543
                     /gene="nrfG"
                     /locus_tag="OQS10_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS2293515.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            10885..12198
                     /gene="gltP"
                     /locus_tag="OQS10_002508"
     CDS             10885..12198
                     /gene="gltP"
                     /locus_tag="OQS10_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS2293516.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12277..12966)
                     /locus_tag="OQS10_002509"
     CDS             complement(12277..12966)
                     /locus_tag="OQS10_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS2293517.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13060..15207)
                     /gene="fdhF"
                     /locus_tag="OQS10_002510"
     CDS             complement(13060..15207)
                     /gene="fdhF"
                     /locus_tag="OQS10_002510"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(14788..14790),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS2293518.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15405..16871)
                     /gene="mdtP"
                     /locus_tag="OQS10_002511"
     CDS             complement(15405..16871)
                     /gene="mdtP"
                     /locus_tag="OQS10_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS2293519.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(16868..18919)
                     /gene="mdtO"
                     /locus_tag="OQS10_002512"
     CDS             complement(16868..18919)
                     /gene="mdtO"
                     /locus_tag="OQS10_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS2293520.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(18919..>19926)
                     /gene="mdtN"
                     /locus_tag="OQS10_002513"
     CDS             complement(18919..>19926)
                     /gene="mdtN"
                     /locus_tag="OQS10_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS2293521.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4435 a         5320 c         5466 g         4705 t
ORIGIN      
        1 acgccagccc caccataaag gcgatgttct gattctcaaa cagcacgccg aggataatcg
       61 caatcacgcc gaggatcagt acggtgattt ttgatacccg cagctcttca cgttcggtcg
      121 cgcctttttt gaagacgtta gcgtacaagt catgcgaaac cgcggatgcg cccgccagcg
      181 tcagacccgc aaccaccgcg aggatagtgg cgaaagcaac cgctgaaata aaaccgagga
      241 acaggttgcc gcccactgca ttcgccaggt gaacggccgc catgttgtta ccaccaatca
      301 gatggcccgc cgcgtcttta tattccggat tcgcaccaac cagcatgatc gcgccgaagc
      361 cgataataaa ggtcagaata tagaagtagc ccataaaccc ggtggcgtag aacacgctct
      421 tacgtgcttc gcgggcatcg ctgactgtaa agaagcgcat cagaatgtgc ggcaagcccg
      481 ccgtaccaaa catcagtccc agacccagag agagtgcgga gatcggatct ttcaccagcc
      541 cgcccggctt catgatgtcg acacctttcg ggtgtaccgc catcgcttca ctgaacagat
      601 tgttgaagct aaagccgacg tgtttcatca ccataaaggc cataaagctg gcaccgaaca
      661 gcaacagcac ggctttgata atctgtaccc aagtggtcgc cagcatgccg ccgaacagga
      721 cgtacatcat catcagcacg ccgaccagca ccaccgcaat gtgatagtta aggccaaaca
      781 gcagctcgat cagtttacct gcgcccacca tctgggcgat aaggtaaagc gccaccacca
      841 ccagagaacc acaggccgaa agaatacgaa tcggcccttg tttcagacgg taagaggcca
      901 catcggcaaa ggtgtagcgc cccaggttac gcagacgttc ggcaatcagg aacaaaatga
      961 tcggccagcc caccaggaag cccagcgagt aaattaagcc gtcatagccg gaggtaaaca
     1021 ccagcgcgga gatccccaag aatgaggcgg cggacatata gtccccggca atcgccagcc
     1081 cgttctggaa gccagtgata ttgccgcctg cggtgtagta gtcgctacga gaacgtacga
     1141 gttttgatgc ccagtaggta atgccgagcg taaacacgac gaaaatcagg aacataataa
     1201 tcgcctgcca gttcgttggc tggcgctcta cggccccgct aatagcatcc gcggcgttag
     1261 ctgcgaaagg gagtgtggcg gcaagcgccg tcagaactct tttcatgatg cttgtacctc
     1321 atgcaggact tcattattaa gacggtcgaa ttcgccgttc gcccgccaga tgtagatacc
     1381 ggtgagaaca aaggagatca caatcactcc aacaccaatt ggaataccgc gtgtgacgct
     1441 ggtgttcgga ttcagcggcg tgccagccag ccgggcgcga aggcgatcag taaaataaag
     1501 ccgatataaa ctgccagcat aataatcgac aggatggtgg caaaccgttg ccgtttttcg
     1561 actaactccc tgaaatgcgc attgtcttct atccgctgat aaatagtgcc attcatcaca
     1621 gattctccag aggtaatgta gggattgttt taattcccgc tcccttatgg gagaaggtta
     1681 acgctcgggt aacccttgcc gaatgtaggc cggataaggc gtttacgccg catccggcaa
     1741 tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgactcgca
     1801 tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc
     1861 gactacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc
     1921 aattttgcgc agaatacggc gcataatttt gccggagcgg gttttaggca gggagtcggt
     1981 ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac
     2041 ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat
     2101 cgcctgacct ttaatattgt gcggaatacc tactacggcg gcttcggcaa tcttcggatg
     2161 cgccaccagc gccgactcaa tctctgccgt ccccagacgg tgaccggaga cgttcagcac
     2221 gtcgtccaca cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc
     2281 gctgaaatac atatttttga aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc
     2341 aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accaggctgc cttcggtagc
     2401 gccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg
     2461 tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc
     2521 agtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca
     2581 gtaccactcc cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga
     2641 cgaacggtcg gtaccctcga tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt
     2701 gggtgcggta tagagaatat tgacctgatg cttgtccacc acctgcgcca tacgggcagg
     2761 cgtcggccag ttgggtacgc cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc
     2821 gtacagcaag taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc
     2881 acccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc
     2941 ggtagtatgc agcacacctt ttggcttacc ggtagaaccg gaggtgtaga gaataaacag
     3001 cggatcttcg gcgttcatct tttccgcctg gtgctgatcg ctcgcttgct caaccaggtc
     3061 gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac
     3121 caccacatgc tctacgctgg tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt
     3181 cagcggaata ctgcgcccgg cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt
     3241 ggaatcaata atgcgcccgg caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg
     3301 caccgcgcca atgcgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat
     3361 ataaatcgcc accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg
     3421 gcagacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc
     3481 ttcccagatg atggcggtac gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc
     3541 cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga
     3601 ggtgtttttc accttctggt aaggtttgat ccagtcaaga atttttccct gttcgcccca
     3661 gaaggtatca ggtacgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat
     3721 caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt
     3781 gttctccttg tagggtgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca
     3841 gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca
     3901 gggtaatttt tgaaggtcag caacaaaagt tgattaattc tttgaggaac atgcagttat
     3961 gcattgctgt tgaaaaagag gaagatacta actaactcta aagtggtatt ttacatgcac
     4021 ttacaattga ttaaagacaa cattcacagt gtggttattt gttacacata ggggcgagca
     4081 atgtcatgac agtgtaggtg cggttactgt cgtgaaaaac aataaaaacc gccattgtaa
     4141 caatggcgca attcggatga agcccctatg acaaggataa aaataaacgc acgccgtatc
     4201 ttcagcttat tgattccttt tttctttttc acttctgttc acgctgaaca aacggcagct
     4261 cccgcaaaac ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca gcatcccgat
     4321 caatatctct cctggaaagc cacctcggaa cagtcagagc gtgttgacgc cctggcggaa
     4381 gatccacggc tggtgatcct gtgggcgggg tatcccttct cgcgcgatta caacaagccg
     4441 cgtggacatg cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa
     4501 aacgctgaag atggtccgct accgatggcg tgctggagtt gtaaaagccc ggatgtggcg
     4561 cgtctgatcc agaaagacgg cgaagatggc tacttccacg gtaagtgggc gcgcggcggc
     4621 ccggaaatcg tcaacaactt aggttgtgcc gactgccata acaccgcctc accagagttc
     4681 gccaaaggca aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc
     4741 attggtaaac cttttgagaa agccggacgt ttcgaccagc aatcgatggt ttgcggtcag
     4801 tgccatgtgg agtattactt cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac
     4861 ggcatgaaag tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc tgactggact
     4921 aactccctgt cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga aacctggaca
     4981 gcgggcattc acggtaaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag
     5041 aacgccgaag gcaaactcta taccgaccat aaaattggta atccgtttga taacttcgcc
     5101 cagacttgtg cgaactgcca tacccaggac aaagctgcct tgcaaaaagt ggtcgcggaa
     5161 cgtaagcagt cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac
     5221 ttcgaagcga aagcagcgct ggatgcaggc gcgacggaag ctgaaatgaa gccaattcag
     5281 gacgatatcc gtcatgccca gtggcgctgg gatctggcga tcgcttccca cggcattcat
     5341 atgcacgcac cggaagaagg tttacggatg ctcggtacgg cgatggataa agcggcggat
     5401 gcacgcacca aactggcgcg cctgctggcg accaaaggca tcacccatga aatccagatc
     5461 ccggatatct caaccaaaga gaaagcccag caggccattg gcctgaacat ggaacaaatc
     5521 aaggccgaga agcaggactt catcaaaacg gtgatcccgc agtgggaaga acaggcacgt
     5581 aaaaacggtc tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg
     5641 gagtgaatat gagcgtatta cgttcgttat taactgccgg ggtgctggcg tcgggcctgt
     5701 tgtggagcct gaacggaatt accgctaccc ctgcggcgca ggcatctgat gatcgctacg
     5761 aagttaccca gcagcgtaac ccggatgccg cctgtctgga ctgtcataaa ccagataccg
     5821 aaggtatgca tggcaaacat gcttccgtta tcaacccgaa taacaaactg ccggtcacct
     5881 gcaccaactg tcacggccag ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc
     5941 gctttaacga gccgatgtac aaggttggag agcagaacag cgtctgtatg tcctgtcatc
     6001 tgccggaaca gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt
     6061 gcgccagctg ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag
     6121 gacggattaa gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc
     6181 cggcgtctgt tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac
     6241 cggcgtcggc gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt
     6301 gaatatcaat ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac
     6361 cgcctgtatg gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac
     6421 gattattcgt agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa
     6481 gtcttgccag cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt
     6541 tcgtgatgcc gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta
     6601 ctgcatcgcc gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga
     6661 caaatgcgat ttctgccgta aaaccaattt gcaggccggt aagctgcccg cgtgtgttga
     6721 ggcttgcccg acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc
     6781 gcaactgctg cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa
     6841 gctgtaccgc gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt
     6901 cattttgaat cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt
     6961 tctgccggtc tggtgacgct ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt
     7021 gcagacagta cgttgctgcg caccacgctg attgtcgggc cgggcgcggt gatcctcggt
     7081 ctgttgatcc tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac
     7141 tacagtttta cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta catggtggtg
     7201 ctggtgctgt ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg
     7261 ttgccgaagc tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga
     7321 ctggaaacgc tgatgctggt gttggcggtg ctgctggggg cttataccgg ctttctgctg
     7381 tcggcgctga aatcgtatcc gttcctcaat aacccgatcc tgccggtgct gttcctcttc
     7441 tccggcatct cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt
     7501 aacccgcatt ccacggaagc gcactttgtt caccggatgg aaatccccgt ggtatggggt
     7561 gaaatcttcc tgctggtggc gttttttgtc ggtctggcgc tgggcgatga cggtaaagtt
     7621 cgtgcgctgg tggcggcatt aggtggcggt ttctggacgt ggtggttctg gcttggtgtc
     7681 gccgggctgg ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc
     7741 attcctgccg tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt
     7801 ttcattctct acgccggaca gttgacggtg gcgtaagcca gaaaagaggt ggtttctgga
     7861 cgtattcctt cctgaagtcg gttttctggc gttgttgtta agtctcgggg tcaacgtgtt
     7921 gaccccgttg acggcctttg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac
     7981 ttgcatcggc attctggcgc agttcgcgct cctgctgctc gcctttggcg tactgacgta
     8041 ttgttttctc atcagcgatt tctcggtcat ttatgtcgcg caacatagcc atagcctgct
     8101 gtcgtgggaa ttcaagctgg cagcggtgtg gggcggccat gaaggttcgc tgctgctttg
     8161 ggtgctgctg ctttccgcct ggagcgcgct gtttgcctgg cattatcggc agcaaaccga
     8221 tccgctattt ccgctgacgt tagccgtttt atctctcatg ctcgccgcac tgctactgtt
     8281 tgtggtgctg tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga
     8341 tctcaatccg atgctgcaac atcccggtct tatctttcat ccaccgctgc tttaccttgg
     8401 ctatggcggt ttgatggtag cggcgagcgt ggcgctggcg agtctactgc gcggcgagtt
     8461 tgatgcggcc agcgcccgaa tttgctggcg ctgggcgtta cctggctgga gtgcattaac
     8521 ggcggggatc atcctcggtt cctggtgggc ctactgcgaa ctcggctggg gcggctggtg
     8581 gttctgggat ccggtggaaa atgcctcttt attaccctgg ctttctgcca ctgcgctgct
     8641 gcacagttta tccctgacac gccagcgggg gattttttgc cactggtcgc tgttgctggc
     8701 gatagttact ctgatgctgt cgctgctggg caccttaatt gtccgttctg gcattctggt
     8761 ttcggttcat gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc
     8821 actgattagc cttgcgtctt tggctctgta tggctggcga gcgcgggacg gtggcccggc
     8881 ggtgcgtttt tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg
     8941 cgcagtgcta ctgatcgtgc tggtgggaac gctttatccg atgatttacg gtctgctggg
     9001 ctggggacgc ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac cgtttggtct
     9061 gttgatgctg gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg cgcagcttcc
     9121 ggcgctggtc gctcatgcgg gcgtgctgtt atttgccgca gggatcgtgg tttccagcgt
     9181 cagccgtcag gagatcagcc tgaatttaca gccgggtcag caggtgacgc tggcaggata
     9241 caccttccgt tttgagcgcc tcgatctgca agccaaaggc aattacacca gcgaaaaagc
     9301 gatagtggcg ctgtttgacc atcagcaacg cattggtgaa ttaacgccgg agcggcgttt
     9361 ttatgaagca cgccgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga
     9421 ctggtatgcg gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc gtttgtatgt
     9481 acaaagcggt gtgcgctgga tctggggtgg aggattgttg atgattgcgg gcgcattgtt
     9541 aagcggatgg cgggggaaga agcgcgatga ataaagggct tctcacgcta ctgctgttat
     9601 ttacctgttt tgcgcgcgct caggtcgtag acacctggca attcgccaat ccgcaacaac
     9661 agcaacaggc gttaaatatt gccagccagt tacgttgtcc gcagtgccag aatcaaaact
     9721 tactcgaatc caatgcgccg gtggcggtca gtatgcgcca tcaggtttat agcatggtgt
     9781 cggaggggaa gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg
     9841 ttcgctataa cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt
     9901 tgttattgct gatggcactg atcctctggc gagtgagggc taagcgatga aacagcctca
     9961 aataccggtg aaaatgctga caacactcac gatattgatg gtattcctct gtgtcggcag
    10021 ttatctgtta agcccaaaat ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc
    10081 gctacatcag tttgccagcc agcaaacccc ggaagcgcag cttcaggcat tgcaggataa
    10141 aatccgcgct aatccacaaa acagcgaaca atgggcgtta ctgggcgagt actatctgtg
    10201 gcaaaacgat tacagcaatt cgctgctggc gtatcgtcag gcgttgcaac tacgtggtga
    10261 gaacgctgaa ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat
    10321 gaccgcccag acttgcgcaa tgatcgacaa agccctcgcg ctggactcta acgaaatcac
    10381 cgctctgatg ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga
    10441 actatggcaa aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga
    10501 gtcgattaat atggcaaaac tgttgcagcg gagatcggat taatcatctg gtgatgaaga
    10561 ttatgtcatt gagctgcata aaaaataatc gaatgaacat atgccaaaaa taatcactaa
    10621 tcagtattat tgcagattaa acaaataaaa atctttccat aacaaatggt tattcattaa
    10681 tcctgccata aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg
    10741 ttgcgcaaca taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa
    10801 cgcaacacaa ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg
    10861 cgctccattg aggaagttat tctgatgaaa aatataaaat tcagcctggc ctggcagatt
    10921 ctgtttgcta tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc
    10981 cgcgactggc tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc
    11041 aaaatgattg ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt
    11101 gatgccaaac agctcgggcg tattggcgcg aaaaccatta tttacttcga ggtgatcacc
    11161 accgtcgcca tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg
    11221 gatatgtcgc agctggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta
    11281 caaagcagtt cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg
    11341 gcgtcgatgg cgaaaggcga aatgctgccg atcatctttt tctcggtgct gtttggtctg
    11401 gggctttctt ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc
    11461 tctgaaacca tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt
    11521 gcgctgattg cggtgacggt ggctaacttt ggtttctctt ctctgtggcc actggcgaaa
    11581 ctggtgctgc tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg
    11641 gcgcgcctgt gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt
    11701 ctggcgtact ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa
    11761 gcctacggag caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac
    11821 cttgatggtt cgacgctgta tcaaagtatt gccgctattt tcatcgctca gctgtatggc
    11881 attgacctgt ccatctggca ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa
    11941 gggattgctg gcgtgccggg cgtgtcgttt gtggtgctgc tggcaacgct gggtagcgta
    12001 ggtattccgc tggaaggtct ggcgtttatt gctggtgttg accgtatcct cgacatggcg
    12061 cgtactgcgc tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa
    12121 cacaaatttg accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat
    12181 aaaactgcgg atcaataatt gaagattgcc ggggatatcc acccggcaat gtgtgaatgc
    12241 ctgatgcgac gcttgccgcg tcttatcagg cctaccttaa ccgttggtta atttttcaaa
    12301 ctcttcacac ccggtatcaa acccttccat acagctcagg ttcaaccagt gcagcgcctt
    12361 ttgcttgttc ttctcgataa aaccttcttc accgtttaag aacatcattc ccgcccagta
    12421 ctcggaataa ccggttcggg aaattgcaga gctgcgtttg aaataccagg ttgccttgtc
    12481 atcgtcggcc ttaatgccca cgccgttggc gtaaatcaaa ccaagcagca tttgggcatc
    12541 gacggcagag tcgttctcca gatcttccga ggcgttttct aacagcgaaa tagcctttgg
    12601 ataatccggt ttacccgctt gagtatttac cagaatatgc gccagcgtta cttcaccttc
    12661 tttactgccc gcttgcgccg ctttctctgc aagaactttt gcttgtggat agtccagact
    12721 gaccggattg gtgattttga tctgcgccag cagcgcgcag gcatcagcat caccgctgtc
    12781 ggcggctttc tgtgcccaat attcggcttt gctcaaatcg ccggagctaa accagctgtc
    12841 ggcaagaaaa tattgtgcgc gtcggtcccc ggcctctgct gcctttaaat actggctgcc
    12901 aggctcggag tcgttggcgt gggcaaagaa tgtcaaaaac aacatcaatg cgataatttt
    12961 tttcattttg ataatacgat tgggtaggga gtaaccagta taaagagttc gaatggataa
    13021 aaaaacagcc tccgaaagga ggctgtagaa aggactgtat tacgccagtg ccgcttcgcg
    13081 caggcgagtt ttcaacttgt tgtactcgtc aatcacgtac tgctcggcgg cgcgctgatc
    13141 ggcgatcggc tcgacgcgca cggcgcagta tttgtattcc ggcgttttcg taatcgggct
    13201 taagttttcg gtaaccagct cgttacaggc accaatccac cactggtagg tcatgtaaat
    13261 cgcccctttg ttcggacgat cgctgacctg cgcacgggtg ataattttgc ctttacgcga
    13321 gtgcacccaa accaatgcct catcttcaat acccagacgt ttggcgtctt cggtattgat
    13381 ttgtgcgtag ccaggttcat cagccagcgc cgccagtgcc gcacagttac cggtcatcga
    13441 acggcaagag tagtgaccaa cttcacgcac cgttgacagt accatcgggt actcgtcggt
    13501 gagtttgtcg attggcgcta cccagtcgca ggtgaagaac tgcgccagac cgttcggggt
    13561 atcaaacttc tctttaaaca gataagaagt cccctgatcg gcatctgaag tatcgcggca
    13621 aggccactga atgaagccca gttcgcccat tttctcgtaa gtcgcaccgt agaaatccgg
    13681 gcacagatga cgcaactcat cccagatctc ctgggtgttg ttgtagtgca tcggataacc
    13741 catacgggtg gcgatttcac tgatgatttg ccagtccgtt ttcagatccc atttcggttc
    13801 aactgctttg aagaaacgct ggaagccacg gtcagccgca gtaaacacgc cttcatgctc
    13861 gccccacgac gttgacggca aaatgacatc cgccgccgac gcggttttgg tcataaagat
    13921 gtcctgaacg ataaccagtt ccagatcttc aaaggcttta cgtactgccg acagctccgc
    13981 gtcagtttgc agcggatctt cgcccataat gtacgcggca cgcacttcgc catgcgctgc
    14041 gcggtgcggc agctcgctga tgcgataacc ggtatgagca ggcaggcttt ccacgcccca
    14101 ggctttggcg aatttctcgc ggttagccgg atctttcacg tactggtatc ccggatacgt
    14161 atccggcagc gcgcccatat cgcaggcacc ctgcacgttg ttctgaccac gaaccgggtt
    14221 tacacccgca tgcggcttac cgaggttacc agtcagcatc gcgaggctgg tcagagaacg
    14281 cacggtttcc acgccctggt agaactgggt tacacccatg ccccacagga tggcggcgct
    14341 tttcgcctgg gcatacatcc gtgccgcctg acgaatctca ctggcgctga cgccggtgat
    14401 atcttcaacc gactccggcg tgtagccttc aacgatttta cgatactctt caaagccttc
    14461 tgtacgggaa gcgacgaacg ctttgtcgta cagattttct tcaataatga catggcccat
    14521 cgcattcaac agcgcgatgt tcgagccgtt tttcagtgca atgtgcatgt cagcaatgcg
    14581 cgcggtttca attttgcgcg gatcgcagac gataattttc gccccgttac gtttagcgtt
    14641 aattacgtga ttcgccacga ttgggtggga atcagccggg ttgtacccga aaacgaacac
    14701 taaatcggta ttatcaattt cgttaatagc attgctcatt gcgccattac cgaccgattg
    14761 gtgcagacct gcaaccgatg ggccgtgtca gacacgagcg cagcagtcaa cgttattggt
    14821 accaataacg gcgcgcgcaa atttttgcat tacatagttg gtttcgttac ccgtaccacg
    14881 cgaggagccg gtcgtctgga tggcatccgg accgtacttc tctttgatgg cgctcaggcg
    14941 ctcggtaacg taattcagtg cctcatccca ggaaacaggt tcgagtttgc cgccacgctg
    15001 gcgacggatc atgggggttt tcaggcgcgg ggtcaggatc tgggtatcgt taatgaaatc
    15061 ccagccataa taacccttca gacacagggt accctggttg gttttcccct gcgctgcctc
    15121 cgcccggacg attttgccgt tatcgacgac caggttgatt ttgcaacctg atgcgcaata
    15181 ggggcaaacc gtgacgactt ttttcatcgg tctcgctcca gttaatcaaa tcacgcatac
    15241 gcgctctcga ctacagtatg catcttttat gccacatttt atttggggtc attccctgat
    15301 attacgggca ctatttattc aaaactctga cgaaaaacag gctgtcgtca gttttgacgt
    15361 gacgaaacga aataccgcgt gacagccatc acgcggcaga cattttattt tttctcgacg
    15421 acgggagccg cctgataccc gccgcccagc gatttcatca actgaatgct ttggatcacc
    15481 cggcggctgt ccagcatcag taatgacatc tcttcggcaa gtactggcaa ccgggcttca
    15541 gtggcctgta agcggctggt taagccgcgc tgataggcgg cctcggcagc gcgctgggtg
    15601 aagcgcgttg cttccacgcg ttcagcctgc atttctcgct cgtcgttgag cgtttgcaga
    15661 cgcgtgccgt tgacggcaac gtcacgcacc gcgttcagta ctgactggtt gtaacgttca
    15721 atcatcatgt tgctggcggc gcgcgtgcct tcgagattgg cattcaaccg tccaccgtca
    15781 aacagcggca atttcagacc cgggatgaag ttgaactggc gactggtttt tttgaataag
    15841 gtatccaggt ggatggcatc aaggccgaaa aacgctttga tatcaaagct cgggtagaac
    15901 agcgcccgcg cggaatccac ctgatctaat gacgcctgaa cataccagcg catggcttgc
    15961 agatccgggc gtctggcgag caactcataa gagagtgtcg ccggaatgcc ggtctggact
    16021 cgtggtaatg ccaccggttt gatctccggc atatcgctgg cccccgcgcc aatcaatgca
    16081 cgcagagatt ctcgcgtttc ggtgatttgc cctttgacgg cagcaatttg tttatcgacc
    16141 gccagaatct gtgcccgcgc gccgtggaaa ggcacttgtg cttccagacc gtgcgccact
    16201 ttactctggt gcgctttcac cgcgtaatca atcacatcgc gagtttgttc taacagatcg
    16261 agcatctgat agctggcctg catactgtaa taaagctgcg ctacgcccgt ggtcagcgat
    16321 agctctactg ctgcggtttc tgccagcgcg gcattatgcg cgccaatggc ggcggcaacc
    16381 gctgagcgat gcacacccca caaatcaaga tccagtccgg caaacaaacc tactgtggct
    16441 tccgtatagt acggcccgtc catacccagt gcgggcgcat ccatcgcata agggcttaaa
    16501 aagccgttcg ccgagacgcg ttggcggttg agcatcccta acgctgcgac ctgtaattgt
    16561 gaaccggcat ctaacaaatc ggcctgggac tgcgcttttt cttcccgcag tttcgcttcg
    16621 gcgagggtgt gtgaaccact tagcgtccgt tggatcagcg catccagctg cgggtcattg
    16681 agttgtttcc accactgcgc ctgcggccag ccggagctgg caagatgaat atcatcggcc
    16741 agtttgattt gttccggttt gagctgttga tgaggcgcag aatccttgcg taccaaggca
    16801 cagccggaaa tcagcgtcgt gctgccgaga atgctgcaca acagcagacg tgaaagttga
    16861 cgattgatca ttgcgtggct ccttgtgcct gttccgtggc gggcgttaat gccggggctg
    16921 tccagtctgg caagcgggcg aaaagctggc agacgtgttg ctcctgtttt aataaggtgg
    16981 gcaggatggc ctgcgaggcg ggtgtctctt caagcgttat ttgaggagaa cggctgagtg
    17041 cggttgccag accggcagcg tatttttcca gggcgtcggc aaactgacct gctctgtccg
    17101 gctgtagtgc gttatccagc gcctgcgccg aattccaggt ggcatcccag gcgtgaagga
    17161 tatcgcggcc ctgacgaata accgtttgcg aacgttcaat cagtaaggcg cgttcttcgc
    17221 tgtccagttg acgctccagc gccacgcgtt ggcacatctc ttcacaggca ttaaacgccg
    17281 catgcagacc gatacgaatt tgcagataag tgcgcagagc cgtgacttcc tgctggcgtg
    17341 ggatccgcat tactttactt aacataccca gcgcgccagc cagtttttgc ggcagcgtgc
    17401 gcgcttcact ttcaggccag acaaaggtgt aaatcaccgc ggacaccacg gtaccaatga
    17461 tgatacccag ggcgcgatcg cgaatttcca ccaggtcgta cactgggcca aaaacgtttt
    17521 cgagcgtggc gagcgcgaag gtgaccacca tctgtgtgcc gatataagaa gagcgttcag
    17581 agctggtggc aatccatgcg cccaacagga aaatcggtgc cagcacaaac agcaattcga
    17641 caatattgtc cagccagggc atgaccagta gcgtgaataa cagcgccaga atcgcgccgc
    17701 aaaaggcccc gccaaaacgc agcaccatct tctggtacga cgaaccgaca tttggattag
    17761 agacgatcac gcaggtcagc atacaggtgt gaatgccttc ccagtccacg ccgctgtaaa
    17821 aggtgtaaca gatcaaacag gcgagcaatg tttttaccgc gtagcgtata tagtctggat
    17881 tggtaaaagc atcggcgacc attgatggcg gtttggcggc gggcgttggc ggcgtattcg
    17941 ggttcatctg gcccagttgt aacaacgtct ggcagatatt ctccaggtta cattcccgtg
    18001 ccgccaccgc ttcactttca ctgatccgcc agtcgctttg ccagcactga ccttcagcaa
    18061 cggcatgctg cagcttgttg atttctgagg ctaatttttg ccgaaattca ataattgcct
    18121 gagaatcagc aaaagaggtg ggatcgtagc gattcagcgt cgagtaaatg taggttaccg
    18181 ttgccacgca gctttgccac catgcgcttt gagttcgcca gttggcatcg tccgcgagac
    18241 aaaagacatt cagtttttgc agcgccagcg cctctctttc aatccgcgtt tcgggtagcg
    18301 gtgcgaggct gtctgtcagg tgactaatgg catcatcaag ccgatcatta agcgcctgat
    18361 gcatttgcga aatggcacga ctgggaaacc acagcacgcc gattaacgtc atcagcaagg
    18421 ttggatagag gccaacaacg atacaccaca gcgttaagcg cacgaccact tccggatagt
    18481 cgagcatggc ggggaaggtt tgcccgtaaa tggcgacaat ggcgacagcg aaaaagacca
    18541 gtcccagccg atgggtgcgc atcaaaaaca tgcggcccat caggatcggc ccggcgatga
    18601 tcaatcggat caacggttcg ccgtatgacc atttgtagat caaaaacagg ctgccgatct
    18661 ccagcaccgt ggcaaccaca aacaagatcg ccacaaattt ggtgtaaaac gcgttcgact
    18721 gaataccgta aaacagcact gccagcgata acgccacaaa agggatctca aaagtcatcg
    18781 agatcagaat caccagcagg cagcctaccc agagttgcac gatctgcggc acgcggccgg
    18841 gtcgccgctc gcttaactct tcatgaaaga acgccagcag cctgaccatc ggtaatggca
    18901 gggagttgag cgcgctcatt attgcggctc aagattagcg actgccgaag cgccgatgcg
    18961 gaacatttcc gggtcaggtt tatcgaccat gattttgacc ggaaaacgct gggcaacgcg
    19021 gacccagtta atagagcgag aaactttcgg caggccgccc agcaccaggc cgccgtcatc
    19081 cggtagcacg ccgtagccaa tcgaatccac tttaccctcg aaggttttgc cgctgtcact
    19141 catcaggcga atcgttgcgg gtgtacctga gcgaatattt ttcagatcgg tttcgcggaa
    19201 gttggcgatc acgtaccagt gacgagtgtc gattagggta aaaatagggc gcatggcaga
    19261 agcaaattgc ccgacggagg ttttgaggga aatgacccgg ccatcaaacg gcgcgcgaac
    19321 ggtcgccatt tccagatgca gtttggtcag ggcaatatcc gcttcgaccg ccgcacgttg
    19381 cgccaccagc gcatcgacgc cgctgacggc gctggcggct gactgcgcct gtaacaatac
    19441 ggcattaaga tcggcttctg cggcgcgctg cgcagttctt gcacggtcaa cgtcttccgc
    19501 tgagacaaaa ccctctttca gtaatggctc ggtacggcgt aatgtatctg tggcctgttt
    19561 cgcggcggca cgggcttttt ctaccgtggc attaatcgag tcggcaccaa actgttgcgc
    19621 atcaacgcta cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcagc
    19681 tttcgccaga ttggcttcgt acgggcgcgg gtcaatgcgg aacagcaaat cgccctgttt
    19741 gactgcctgg ttgtcggtga ccgccagttc tacaatgcgg ccgctgactt ccggcaccac
    19801 atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca
    19861 gataacgaaa acaagggcaa ccagcgccaa tgcaaccact aacagagcag ggaatttact
    19921 gcgagg
//
LOCUS       DAJVXP010000075        19378 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVXP010000075 DAJVXP010000000
VERSION     DAJVXP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.075"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            198..274
                     /locus_tag="OQS10_002514"
     tRNA            198..274
                     /locus_tag="OQS10_002514"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:232..234,aa:Arg,seq:acg)
     gene            555..1121
                     /gene="yqaB"
                     /locus_tag="OQS10_002515"
     CDS             555..1121
                     /gene="yqaB"
                     /locus_tag="OQS10_002515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS2293522.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            1118..1546
                     /locus_tag="OQS10_002516"
     CDS             1118..1546
                     /locus_tag="OQS10_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS2293523.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1619..3175
                     /gene="gshA"
                     /locus_tag="OQS10_002517"
     CDS             1619..3175
                     /gene="gshA"
                     /locus_tag="OQS10_002517"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS2293524.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3325..3840
                     /gene="luxS"
                     /locus_tag="OQS10_002518"
     CDS             3325..3840
                     /gene="luxS"
                     /locus_tag="OQS10_002518"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS2293525.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3904..5442)
                     /gene="emrB"
                     /locus_tag="OQS10_002519"
     CDS             complement(3904..5442)
                     /gene="emrB"
                     /locus_tag="OQS10_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS2293526.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5459..6631)
                     /gene="emrA"
                     /locus_tag="OQS10_002520"
     CDS             complement(5459..6631)
                     /gene="emrA"
                     /locus_tag="OQS10_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS2293527.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6758..7288)
                     /gene="emrR"
                     /locus_tag="OQS10_002521"
     CDS             complement(6758..7288)
                     /gene="emrR"
                     /locus_tag="OQS10_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS2293528.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7379..7714)
                     /gene="ygaH"
                     /locus_tag="OQS10_002522"
     CDS             complement(7379..7714)
                     /gene="ygaH"
                     /locus_tag="OQS10_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS2293529.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7704..8441)
                     /gene="ygaZ"
                     /locus_tag="OQS10_002523"
     CDS             complement(7704..8441)
                     /gene="ygaZ"
                     /locus_tag="OQS10_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS2293530.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8565..9749)
                     /locus_tag="OQS10_002524"
     CDS             complement(8565..9749)
                     /locus_tag="OQS10_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2293531.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(10040..11032)
                     /gene="proX"
                     /locus_tag="OQS10_002525"
     CDS             complement(10040..11032)
                     /gene="proX"
                     /locus_tag="OQS10_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS2293532.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(11090..12154)
                     /gene="proW"
                     /locus_tag="OQS10_002526"
     CDS             complement(11090..12154)
                     /gene="proW"
                     /locus_tag="OQS10_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS2293533.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(12147..13349)
                     /gene="proV"
                     /locus_tag="OQS10_002527"
     CDS             complement(12147..13349)
                     /gene="proV"
                     /locus_tag="OQS10_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS2293534.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13705..14664)
                     /gene="nrdF"
                     /locus_tag="OQS10_002528"
     CDS             complement(13705..14664)
                     /gene="nrdF"
                     /locus_tag="OQS10_002528"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS2293535.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14674..16818)
                     /gene="nrdE"
                     /locus_tag="OQS10_002529"
     CDS             complement(14674..16818)
                     /gene="nrdE"
                     /locus_tag="OQS10_002529"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS2293536.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16791..17201)
                     /gene="nrdI"
                     /locus_tag="OQS10_002530"
                     /pseudo
     CDS             complement(16791..17201)
                     /gene="nrdI"
                     /locus_tag="OQS10_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(17198..17443)
                     /gene="nrdH"
                     /locus_tag="OQS10_002531"
     CDS             complement(17198..17443)
                     /gene="nrdH"
                     /locus_tag="OQS10_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS2293537.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17691..18020)
                     /locus_tag="OQS10_002532"
     CDS             complement(17691..18020)
                     /locus_tag="OQS10_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS2293538.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            18172..18516
                     /locus_tag="OQS10_002533"
     CDS             18172..18516
                     /locus_tag="OQS10_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS2293539.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18553..19002)
                     /gene="alaE"
                     /locus_tag="OQS10_002534"
     CDS             complement(18553..19002)
                     /gene="alaE"
                     /locus_tag="OQS10_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS2293540.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4869 a         4884 c         5006 g         4619 t
ORIGIN      
        1 attctccgaa atcttcagca atgaaggtat cgaagaggta gcggaattaa ccgcaggcac
       61 cagggatgat aacgttgctt tagcaacggc ccgaagggcg agccgcaagg cgagtaatcc
      121 tcccggatgc accatctctt aattgatatg gctttagtag cggtatcaat atcagcagta
      181 gaataagttt tcccgatgca tccgtagctc agctggatag agtactcggc tacgaaccga
      241 gcggtcggag gttcgaatcc tcccggatgc accatattct ccgtaacctt cagcaatgaa
      301 ggtatcgaag agatagcggt attaaccgca ggcaccagga aggataacgt tgcgccagca
      361 acggcccgaa gggcgaacgt cagtgagtca tcctcccgga tgcaccatct tctccaaaaa
      421 atttccttca aaaatatctc atttctaaat aactccttta tccatttcct gaattttctt
      481 ctccttcgcc atcagcgaca aaatcaagca attacgctaa agtagcgtca tatttttcga
      541 cttgcgagaa gaccatgtac gagcgttatg caggtttaat ttttgatatg gatggcacaa
      601 tcctggatac ggagcctacg caccgtaaag cgtggcgcga agtattaggg cactacggtc
      661 ttcagtacga tattcaggcg atgattgcgc ttaatggatc gcccacctgg cgtattgctc
      721 aggcaattat tgagctgaat caggccgatc tcgacccgca tgcgttagcg cgtgaaaaaa
      781 cagaagcagt aagaagtatg ctgctggata gcgtcgaacc gcttcctctt gttgatgtgg
      841 tgaaaagttg gcatggtcgt cgcccaatgg ctgtaggaac ggggagtgaa agcgccatcg
      901 ctgaggcatt gctggcgcac ctgggattac gccattattt tgacgccgtc gtcgctgccg
      961 atcacgtcaa acaccataaa cccgcgccag acacattttt gttgtgcgcg cagcgtatgg
     1021 gcgtgcaacc gacgcagtgt gtggtctttg aagatgccga tttcggtatt caggcggccc
     1081 gtgccgcagg tatggacgct gtggacgttc gcttgctgtg agtgaagcgt tatcgctttt
     1141 ctcgttgttt gccagtagtt ttctcagcgc tacactatta cccggcaact ccgaagtcgt
     1201 gctggtggca atgttgcttt ccgggatcag tcatccctgg gttttagttt taacagcaac
     1261 aatgggtaat agccttggag ggttaactaa cgttatcctt gggcgtttct ttccattgcg
     1321 taaaacatcg cgctggcaag agaaagctat cggctggctg aaacgctatg gtgcagtcac
     1381 gctattatta agctggatgc ccgtggttgg cgatttactg tgtctgttag cgggatggat
     1441 gcgcatctcg tggggaccgg taatcttttt tttgtgcctt ggtaaagcgt tacgctatgt
     1501 tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg cactaattgt aggcctgcac
     1561 atatggtcac cattacagtt atgctaatta aaacgatttt gacaggcggg aggtcaattt
     1621 gatcccggac gtatcacagg cgctggcctg gctggaaaaa catcctcagg cgttaaaggg
     1681 gatacagcgt gggctggagc gcgaaacttt gcgtgttaat gctgatggca cactggcaac
     1741 aacaggtcat cctgaagcat taggttccgc actgacgcac aaatggatta ctaccgattt
     1801 tgcggaagca ttgctggaat tcattacacc agtggatggt gatattgaac atatgctgac
     1861 ctttatgcgc gatctgcatc gttatacggc gcgcaatatg ggcgatgagc ggatgtggcc
     1921 gttaagtatg ccatgctaca tcgcagaagg tcaggacatc gaactggcac agtacggcac
     1981 ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg ctgaaaaatc gctacggcgc
     2041 gctgatgcaa accatttccg gcgtgcacta caatttctct ttgccaatgg cattctggca
     2101 agcgaagtgc ggtgatatct cgggcgctga tgccaaagag aaaatttctg cgggctattt
     2161 ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt ccttatctgt ttggtgcatc
     2221 tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg tcgctgccgt ttgagaaaac
     2281 cgagtgcggt atgtattacc tgccgtatgc gacctctctt cgtttgagcg atctcggcta
     2341 taccaataaa tcgcaaagca atcttggtat taccttcaac gatctttacg agtacgtagc
     2401 gggccttaaa caggcaatca aaacgccatc ggaagagtac gcgaagattg gtattgagaa
     2461 agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag attgaaaacg aactgtatgc
     2521 gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca ccttctgatg cgctgttacg
     2581 tggcggcatt gaatatattg aagtgcgttc gctggacatc aacccgttct cgccgattgg
     2641 tgtagatgaa cagcaggtgc gattcctcga cctgtttatg gtctggtgtg cgctggctga
     2701 tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc gttaactgga accgggtgat
     2761 ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc tgcgaaaccg cgcagttccc
     2821 gttactgcag gtgggtaaag atctgttccg cgatctgaaa cgcgtcgcgc aaacgctgga
     2881 tagcattaac ggcggcgacg cgtatcagaa agtgtgtgat gaactggttg cctgcttcga
     2941 taatcccgat ctgactttct ctgcccgtat cttaaggtct atgattgata ctggtattgg
     3001 cggaacaggc aaagcgtttg cagaagccta ccgtaatctg ctgcgtgaag agccgctgga
     3061 aattctgcgc gaagaggatt ttgtagccga gcgcgaggca tctgaacgcc gtcagcagga
     3121 aatggaaacc gctgataccg aaccgtttgc ggtgtggctg gaaaaacaca cctgacagaa
     3181 aagaaaaagg ccactcgtga gtggccaaaa tttcatctct gaattcaggg atgatgataa
     3241 caaatgcgcg tctttcatat actcagactc gcctgggaag aaagagttca gaaaattttt
     3301 aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata gcttcacagt cgatcatacc
     3361 cggatggaag cgcctgcagt tcgggtggcg aaaacaatga acaccccgca tggcgacgca
     3421 atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag aagtgatgcc agaaagaggg
     3481 atccataccc tggagcacct gtttgctggt tttatgcgta accatcttaa cggtaatggt
     3541 gtagagatta tcgatatctc gccaatgggc tgccgcaccg gtttttatat gagtctgatt
     3601 ggtacgccag atgagcagcg tgttgctgat gcctggaaag cggcaatgga agacgtgctg
     3661 aaagtgcagg atcagaatca gatcccggaa ctgaacgtct accagtgtgg cacttaccag
     3721 atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca ttctggaacg tgacgtacgc
     3781 atcaacagca acgaagaact ggcactgccg aaagagaagt tgcaggaact gcacatctag
     3841 tcagtaaact atcttcacaa ttaattgaaa aaagccagtt caaatgaact ggcttagttg
     3901 tacttagtgc gcaccgccgc caccgccacc tgcgccaaat ggcggtttag caaaccacac
     3961 cagccccagc agtacgagga atatcccggc tgacatccag aagatctcat tggcggaaat
     4021 aatcagcccc tgattggtga tctgctgggc aatccagcct gatgcctgct gttgcgtcat
     4081 cccaagccct tccagttgac tgtacatcgc ctgggcattc gggttgaacg ggtttaccga
     4141 ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc cacatggtcg tggttatcga
     4201 cgtgccgata gaccccgcca gcgttcgcgt aaagttagag aggctcgatg ccgccgccag
     4261 tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc ggcataaaga agcaggccac
     4321 cgcaaacccc tggataaact gcggccaggc cgacgcgcca aaatccatac ctggttcaaa
     4381 ggtataggca cgccagtaga agcagacggc atacataata aagctgaagg ttaccagccg
     4441 ccgcatatcc agtttatgcg cgaagcggcc gataatcggc gacaggatca ccggaataat
     4501 ccctaccggc gcagaggcca aacctgccca ggtcgccgtg taaccgtaga cctcctgcaa
     4561 caactgcggc agcagaacaa tagcgccgaa gtagagcata tacgcgaggc tgatacacaa
     4621 gcagccgatg gtgaagttgc gcgacttaaa caacgacaga tcgactatcg ggttatcgtc
     4681 ggtcagctcc cagacaatca ggaagcagat agccaccacc gccaccacgg taaggatgat
     4741 aatttcctgt gatgaaaacc agtccagctc tttaccgcgg tcgagcataa tctgcaggct
     4801 gccgatacca ataaccagca gtgccagccc cacggcatca atccgccgcc gtttggtgcg
     4861 ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc accgccacgc caatcggcac
     4921 gttgatgaag aatatccagc cccagtggta attatcgctg atataaccgc cgaggatcgg
     4981 gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt
     5041 ggctggcggg tagttattca gcaatagact ttgcgaaagc gggatcaacg gcccggcgac
     5101 aatcccctga atcacgcgga agaagatcag catattcagg ctgctggaga caccacacgc
     5161 ccacgacgca atagcaaagg cgatggtgga ccaaaggaac agtttcactt ccccgacgcg
     5221 ctttgccagc cagccggtaa gcgggatcga gatggcattc gccaccccga aagaagtgat
     5281 tacccacgtt ccctggctga gcgatgagcc cagattcccg gcgatagtgg ggatcgccac
     5341 gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat
     5401 aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca cctctggatt
     5461 agccagcgtt agcttttacg atatcgtcga tcagtttatt gacaggtgcc aggctgattt
     5521 cacgcgcggt acttaccgcg accggtgttg aacgtacttt atttgccagt acctgaccgt
     5581 cacggttagt ggtattgacg ctcaccagtg tggacaaacc aatgcgcagc gggtattgct
     5641 ccagctgttt ctggtccagt tcgatacgca caggtagacg ctgaacgact ttgatccagt
     5701 taccggtcgc attttgcgcc ggaagcagtg agaatgcgct acctgtgccc atatccagac
     5761 caaccacttt accggtgtat ttcacatcat cgccgtaaat atccgtggtg atagtgaccg
     5821 gctgaccgat acgcatattg gcaatctgcg tctctttaaa gttggcatcc acccacatat
     5881 tggtggctgg aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc ccaggctgta
     5941 ccgcacggcg ggagacataa ccggtcatcg gactgacaat acgggtacgc tccagcgcca
     6001 gccaggcgtt acgtacttcg gtggcagcct gttgcacggc aggctgatct tccagtttag
     6061 tccccagaat catcgcctga ttggcattgt attgttgaat agcgacgtcc agttgcgcct
     6121 gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat
     6181 tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt tgcgagggcg attttctgca
     6241 cctcaatatt cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc
     6301 tggaagccag tgcagttttg gctttttcaa acgcctggcg agcatctgtc gggtcgagag
     6361 tgaccagcac gtcgccttct tttacaaaat cggtgttatc ggcccagact ttcgtcacgc
     6421 tgccagacac ctgagacata atttgaatct gattccctgc cacgtatgcg tcatcggttt
     6481 cttcgaaatg acgcagtacc aaaaaccaat aaatccctat cgctacggca ataattataa
     6541 agagcaaggt gagaaggagg agcagacgct tacgtttgcc gctcttcttt accggttgct
     6601 gcggggtttg agtctccgca tttgcgctca tattgttctc cacgatcttc ttaactcatc
     6661 ggctgagccg acctgcttgc caaaaaggcc agcatgttgc gatgctggcc ggtcattttt
     6721 tcttttataa atctggattt ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca
     6781 caccgtcttg ttccatctgg tcgagacggg agagcaattt gcgggtgatt tgctcgagct
     6841 gatctttttc tgttgtgctg agcgcggacc agagttgatg caggcagtta tgctgcggtg
     6901 gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc aggcagcggc
     6961 gatcgttatc gctttcacga cgttcgatcc aaccgcgttt ttccagttca tcggcaatac
     7021 gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa ttcagaaggc tgaatactgt
     7081 ggttttcctg agactccagc gtaatcaacg ccataaacaa cgtctcgtta atcccctgag
     7141 ccttcagcat tttattgcgg ttctccagca gcttgctttg catgtgcatg caaagacgag
     7201 tcagaaggat ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt
     7261 gttcaatggg cgtaaacgaa ctatccattt gggtatgacc tcattaattt cagccgatat
     7321 agtaacgaca gtgacaaata aagtaaatgt attgttttag aaaaatgatt cttgtgggtt
     7381 atataatcgc catcactttc caggcgagcc cataggccag cgcactaagc agtgttggga
     7441 tgataatgct gcgtgtttta tagaaactgg cacccagtac cgcgaagccg accagcgtgg
     7501 gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc agcagagcgc
     7561 atatcgaggc gatgccaatg gtgtcgagca aaatacctac cgcgccacgt ttggttgggc
     7621 gggcattacc cacacgcagg cgcagcggca aatagcggaa gcaataattc gccgcgccaa
     7681 ccagtaaccc aggcagcaga acctcatagc tcatcgggcg ctccttgcca gaatgcttgg
     7741 attaacgcag tgaggcagcc acagacaatg cctgccagaa tggcgacggg aatagaaaat
     7801 agcgttacgc ctgcaagggc accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc
     7861 tggaaagagg cgagcaggaa actcataaag agtgccggaa gcataaaacc taatgctgct
     7921 tcaacggcgg gataaccttg cagcaagccg ctgccagaga atgcccctat taccgtacca
     7981 aatacccacg atgaccatga actgaaggca atgccgatca tccagttctc gctccagcgg
     8041 cgattattgc gtaccagttt tgctgttgcg gcggcaaaaa cctcatccgt caggccaaac
     8101 gcccacaggg cggttttcga tttttgcaga cgctgaataa tacggctacg cagtgacggg
     8161 ccatacaaca catggcgaac atccattgcc atgacggtca gtgcagcaac ccacaaacta
     8221 ctcccggctg ccagcatcgc ggtaatgacg aactggctcg cgcctgcata aatgatgcag
     8281 gagaaaaaaa cgctttcgag aggagagaat cccagacggg tcgcattcag accgaacgca
     8341 aaggccaccg gaatataact aataacaatc ggtaaactgt ctttgcatcc ttccatgaag
     8401 gtcgccgaac caggagcagg ctgtggagta gggctttcca tagagtgtac gcttaacgat
     8461 tgttaatatt ataaatagac tgaatgaata tcttaacctt atcagactga tgggcttctt
     8521 aacaccctta taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt tgcggcttcc
     8581 tgacgatgaa aacctcgcca ccagaccagt aagttaacta gggcaatcgt cgcgccagcc
     8641 agacaaacgc cagcccaacc gccatgttgc caggctgagg ctgaaattag cgaaccggcg
     8701 gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg
     8761 tgtattcgat aaattaccgt ctggttagtg atatgcacgc cctgtacggt gagatccagc
     8821 accaggatgc cgataatcag cgccagtacg gaagtgtgac caaaccagat cgccagccat
     8881 gaaagtaata gcagcagcag accgaaagtt gtggtgtggt gcgatttacc cttatcggca
     8941 aaaccgcccg ccggacgagc gcccaacgct ccggcagctc ccgcaagtcc aaacagacca
     9001 atgacaccat cgctgtagtt aaaaggtgga gcggcaagca aaaaggccat tgaggtccag
     9061 agaatgctga agttggcaaa ggtcaggcag cccagcaatg cgcgggtgcg gaggatctta
     9121 tcgctgataa acatgctgaa aacggaacct aacaactgtg ggtagttgag gtgggtttct
     9181 gatttcattt gtggcagacc acgccataat gctagcgcca tcagtgccat taacaccgag
     9241 gcaacccaaa agacggtgcg ccagccgccg aggtttgcca gtaacccggc aacggttcgt
     9301 gccagcaaga tccccaacag cagaccgctc ataatggtgc caaccacttt gccgcgttta
     9361 tccggtgaag ccagcgtcgc tgccagcgga accagaattt gtgccacgac tgagaataaa
     9421 ccggttaatg ccgtaccgag gatcatcatc gccagcgact gactgctggc agtgattaac
     9481 atgccgcctg ccgccagtaa ggtcatcgag acaatcaggc ggcggcgttc aaacatatca
     9541 ccgaggggaa caagaaacag cagacctgct gcatagccca actgcgcggc ggtaacaata
     9601 aagcctgccg aactggcgga aagggaaaag ttacgcgcga tggtgtcgag caatggctgg
     9661 gcgtaatagt tactggctac cgcctgaccg gtggcgatag acatcagcac gatcagcgcc
     9721 gggctaagct catgattagg tttagtcatt atttttgttc atcaggtgat gattaagctg
     9781 gaaatcaata atagcggatg ttgatgaatg ggggcgaaag atagcttgtt gaaatgtgcg
     9841 atgtggtttg taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaacg
     9901 gcggatgcgg cgaaaacgcc ttatccgcca tacggaatga gtgtattttg taggcatgat
     9961 aagacgcgcc agcgtcgcat caggcattgt gcacagaatg gcggatgagg caaaaacgac
    10021 ttatccgccc gaataaaaat tacttctgcg ctgccagcgc ctcattcacc cagccatcga
    10081 actgctgctg gtgggctttg atccaaccat caacatggcc ctgaatatcg ccttctgagg
    10141 ctttgccgtc atgcataatg gcgttctggg cgttaatatc tgccactggc aactgcataa
    10201 tggcaaaaag tttcgctgct gccgggtttt tctcggccca ggctttgttg gcaacgatat
    10261 gcatggtgct gaccgggaag ccataattcg caccattcgg cagtttggta tcggcgtttt
    10321 tatcgcccgg cagtgcggag aacggcacct gcaaccagac cacatctttc cctggcttca
    10381 gctcattact cacccagtac ggcgtccagg tgtaataaaa caccggtttg ccctctttgt
    10441 agcgactgat ggtgtcggcc atcatcgctg cgtagttccc ctgattatgc gtcacggtgt
    10501 tggtcagttc atacgcggca agctggtggt tgatcgcacc ttcgcagccc cagcctgggt
    10561 tacagccggt taaatccgct ttaccgtcgc cgttggtatc gaacagtttg gcgatcttcg
    10621 gatctttcag ttgtgcgatg ttggtgattt tgtactggtc ggcggttttc ttatcgatca
    10681 ggtaaccctg tgccgcgccg ttaacgaata tcccttcaca ataaaatttc ttatcgccac
    10741 cggcagcttc gtacatgttg tcatgcagcg gcgtccagtt cacggcggtg aaggttgcat
    10801 cgccggaagc aagtgaggtg tagccaacgt tgtaatccac ttcgctgggt ttattgacgg
    10861 tataacctaa tttctccagt gcacgactga ccagcagcgt ctggaaggtt tcttcagtga
    10921 tggtgctctg aactggatta acagtaatgc ctttgcccgg cagatcggct gcaaaagttt
    10981 gtgtagagat aagcgtggca aacgctgtcg caaaaagtac gctatgtcgc attgttattc
    11041 ctttttttgg cagggtgata atgcccggcg ttaccgggca agtgcagagt tacttaatga
    11101 atgggcgggt cagcagaccg acagggccag tggtgtacca gcgacggttg ccgcgactgc
    11161 gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat aatggcgagg atcacaatcc
    11221 cgacgccgcc aacggtggca aggcccatat ccagacgacc gataccgcga agtaccatct
    11281 gacccagccc gccgacggca atcatcgagg cgatgaccac catagaaaga gccagcatca
    11341 gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag cggtaactga actttgaaca
    11401 gcatctggcg cgggctggca ccgaatgagc gcgaggcttc aatcagatcc gccggaacct
    11461 ggttaatccc cagaatcgtc agacggataa tcggcggcag agcaaagatg atcgtcacca
    11521 ctacgcccgg cacgttaccg ataccaaaca gcatgacgat tggtaccaga taaacaaacg
    11581 ctggcgtggt ctgcatggca tcaagcagtg gacgaataat tttcgccgct cgcggacttc
    11641 tcgccagcca tatccccaac ggcaaaccga tgacgataca gaacagcagg gcggttaaca
    11701 ccagcgccag agtcaccatt gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg
    11761 aaaccagcgt cgccacgccc attccgaccc cggaaatctg ccaggcgatg agagcgaaaa
    11821 cgataatcgc cactggcgct ggcataccca gcagcaattg ctggaaactg ttgaggatat
    11881 aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa atgggtaaca acccagtcga
    11941 tcccttcagt gacccaactg tcgagcggga tcagcgtttt atggaacgga tcgagaatat
    12001 taaaatgctc gacgtttggc gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag
    12061 tcggtgcagt cgtcggtgta ccccaggcgt ctgcggattg cgcggcactg tccgccgctg
    12121 gcgtggtatc ccacggatta ttttgatcag ccattattta ccccctcacg atctaaagcg
    12181 cgcagcagca ttcctttcga aatgatgccg acatactgtt ggtcctcgtc gaccacgggc
    12241 accgcacagg gtgcctgtcc gacatgagag agcaactcgc taagaggcgt ttgtgcatcg
    12301 actgctaacg gcgcatcaat cagcgccgca tcaagacctt gctgctgcgt taacgcggtt
    12361 ttaagcgaat cgatggagac tgcgccgaca aacttattac cgcgttcgat aacgtagcca
    12421 tattcgcgat cttcatcctg caataatttc agtgccgaac gtgggccgaa gccaggggtt
    12481 ttacgaatta agccattcgg tgtccggcgg gcaatatctt tcgcactgaa tacctgacta
    12541 atatcaacgc cacggaagaa ggtacggaca taatcattcg ccggattatt gagaatttca
    12601 tccggtgtgc cgacctgtac cacttcacca ttttgcataa tggcaattcg gtcgccaata
    12661 cgcatggctt catcgagatc gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt
    12721 aattttacca gctcatcctg catctcggtg cgaattaatg gatcgagcgc cgagaaggct
    12781 tcgtccatta ataaaatatc cggattaatc gctaacgcgc gggctaatcc cacacgttga
    12841 cgcatcccgc cagagagttc atccgggtag ctgtgggcat aattttccag cccgacctga
    12901 cgcagtgcat caagggcttt ttcccggcgt tcttcggcat taattccggc caattccata
    12961 ccgaacgcag tattgtccag cacggtcata tgcggcatta aggcaaagga ctggaagacc
    13021 atcgcaatct tttttctgcg cacctcacgg agtttggcat cagatatttt ggcaatatcc
    13081 acaccatcaa tcagcacttg cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc
    13141 attgtggatt tacccgagcc ggataatccc atgatgacaa atatctcgcc ttcttcaatg
    13201 gccagactgg cgtcttttac gccaagcgat agcccagttt tttccagaat ttgttctttt
    13261 gaaagtcctt gttcgatata tttgaatgct cgctgtggat gctcaccaaa tattttataa
    13321 aggtttttaa tttctaattt aattgccatg caatagaaag attcctgtta tttgtttatg
    13381 tcgatatatt acccaatgga aatagttact tttttctacc ctaacatact gagaatctga
    13441 ggcaacccct gatggcaaat gtggcatgaa tattgatgcg agcaaatcgc cggaatttcc
    13501 cgtgatataa gggctgagag caaatcgaga aaagttgata ttttttgtca aagatagcga
    13561 aaaaacgcct gaatattgtg gtttcaggcg aataatgcaa cagagtatca gtgtagatca
    13621 ccacaaaata tttgcgtgat aaaaagctat ttggcgggaa ttatttccct gtcgcgggta
    13681 gtgatatttt tgaaaataac accctcagaa attccagtct tcatcttctg tttcaaccgc
    13741 tttccccatc acataagagg agcctgaacc ggaaaaaaaa tcgtgatttt catcggcatt
    13801 cggcgaaagc gcggcgagga ttgccggatt cacttccgcc atttctgcgg gaaataaagg
    13861 ttcgtagccc agattcatca aagccttatt ggcgttgtaa cagagaaacg ctttcacatc
    13921 gtcagcccac ggggtttcgg cgtacagctc atcggtgtat tgcagctcgt tgtcgtagag
    13981 ttccagcaac aaatcgaagg cgaaactctt caactcttca cgttgtccca gagatatctt
    14041 ttccatgttt ttctgatatt tatagcctat gtagtaaccg tggactgctt catcacggat
    14101 aatcagacgg atcaggtctg cggtattggt cagctttccg cggctggaaa aatacatcgg
    14161 cagccagaaa ccggaataga acaaaaaaga ttcaagaaac acactggcga ttttcttttt
    14221 cagcggatca tcaccgcgat aatgttgctg aataatctga gcttttcgct gcaacggtgc
    14281 gttttcttca ctccaggcgt aggcggcatc gacatctttg gtctggcaca gcgtcgagaa
    14341 aatcgaactg taagagcggg catgaaccgc ttccataaag ctgatattcg ataataccgc
    14401 ttcttcatga ggcgtgagtg catcgggcat cagcgaaggc gcgccgataa cattttgcag
    14461 cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc agttgttgtt ctacgacagt
    14521 taatgtctgc caggcaggaa tatcgttcga cagcggcacc ttttctggta gccagaaatt
    14581 gctggtcagg cgattccaca cctccagatc tttatcgtca gatatcttgt tccagttgat
    14641 ggcgctgata cgtgagagtt tcatagatat tccttaaagt gcacaggaga cgcagccttc
    14701 aatttcagtg ccttccagcg ccatctgacg caggcgaatg taatagagcg ttttgatacc
    14761 tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga gtggtggcgg tatcggggaa
    14821 aaacagcgtc agcgacaaac cctgatcgac atggcgagtc gcttccgcgt aggtgtcgat
    14881 gatcttttct gcgccgattt cgtaagcatc ctgatacagc gccagatttt cgttagtcat
    14941 aaacggggca gggtagtaaa cgcgtcctgt tttgccctct ttgcgtatct ctactttcgc
    15001 cacaatcgga tgaatactcg acgtagcatg gttgatataa gagatagaac cggttggcgg
    15061 caccgcctga agattctggt tgtaaatgcc gtagcgcatc acgtcgtcgc gcagttgcgc
    15121 ccacatctca cgggtaggta acgtaatacc gctgcgggca aacagttcgc caactttcgc
    15181 cgttttcggc tgccagttac cttgcagata ttggctaaaa tattcaccac tggcataacg
    15241 tgactgtttg aacccggcga aggtttcacc gcgttcgcgc gccagcaaca tcgaggtacg
    15301 cagtgcgtgc caggtgatgg catagaaata gagattggtg aaatccagtg cttccggcga
    15361 accataagcg atgccttctc gcgccagata gccgtgtaaa ttcatctgcc ccagtccgat
    15421 ggcgtgtgag gcggcatttc cggcttcgat ggacggcacg ctgcggatat gactcatatc
    15481 tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg cgggcaaaat cgggggaatc
    15541 catggtgtgc gcaatattca acgaacctaa attgcaggag atatcatggc ctgtgcgggc
    15601 atagtcgaga ttctcgtcat actctgaggc acagttgacc tgcaaaatct ctgagcagag
    15661 attactcata tttatgcgcc cggcgacagg gttagcacgg tttaccgtgt cttcatacat
    15721 gatgtaggga tagccggact caaactggat ttctgccagc cgctggaaga aatcacgggc
    15781 gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt tcgtcatagt gttcgctgat
    15841 ggcgatatcg gcaaacggct tgccataaac tcgctcaacg tcatagggcg aaaacagcgc
    15901 catctgcgca ttctcttttg ccagatggaa agtgatatcc gggatcacca cgcccagcga
    15961 cagtgtttta atgcggattt tttcgtcggc attttcccgt tttgtatcga gaaaacgcag
    16021 aatatcggga tgatgagcat gtaaatagac tgcacctgcc ccctgacgcg cgccgagttg
    16081 gttggcatag gaaaatgcgt cttccagcat tttcatcacc ggaattacgc cagaagattg
    16141 attttcaata cgtttaattg gcgcgcccgc ttctcgcaga ttcgacagca aaaatgccac
    16201 gccgccgccg cgtttcgaca gttgcagtgc ggaatttacc gcccggccaa tcgactccat
    16261 attgtcttca atacgcagca gaaaacagga aaccagttcg ccgcgctgct gcttaccgca
    16321 gttgaggaat gtcggcgtgg ctggctgaaa gcgtcctgac agcatttcat cggtcagttg
    16381 caacgccagc gtcgcatcgc cttgtgccag cgtcagcgcc accatcgtga ctcgatcggc
    16441 aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac gtatagctgg tgtaaaactt
    16501 ccatgccccg aggaatgtct ggaaacgaaa accgctggtg tgtgcgtggg caaacagcgt
    16561 aatgacaaag tcgcgagagt agcgattaag aacgctttca tcatagtaac cttcgttgac
    16621 cagccagttc aggcgctgct gctggctact gaaggtcaca ctgttcggac gcacatgcgt
    16681 cgccataaag gcgtcaacgg cctggcggtc tttatcgaac tgaatgcgac ctgcgctatc
    16741 gtagaggtta agcatcgcat tcagcgcgtg gtaatccatc gtttcctgcg tcaggcattc
    16801 tgcggttgtc gttgccaaaa ttcggttact cctttacgaa cgttttcgat atcgctttgc
    16861 gtacccatga gttcaaaacg gtacagccac ggcacgccgc atttccgggc aatcacatct
    16921 ccggcgcggc catacgcctc accaaagttg cgattaccag aagcaataac gccgcgaagc
    16981 aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct atcgtggcac cgcgccagcc
    17041 gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag gctcgtctac ctgaatccgt
    17101 tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta aacgttcgat aaaacgctgc
    17161 gtgttttcgg agctgctgga gaagtagacg agctggctca tgcactggcc gcgtgtggcg
    17221 ctggatgcag acggttaatc atgtccggac ggaaaccaga ccagctaaga tcgccagcaa
    17281 tcactaccgg caactgacga aagccctgag cacgcaacgc ttccgctgct tcaggaacgc
    17341 gatcgacatt aatcatttca aaatcaaagc cccggttttc catcgcccgt ttggtggcgt
    17401 ggcactgaac gcaatcgtta cgagtgtaaa tagttatgcg catgattcgt atttccgttt
    17461 aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct ctctgttgat acagagatac
    17521 tagatgtagt tgaaaaaaga ttcaaccaca caatatatag caaatagtga tcgaaattac
    17581 cctagatatg agcgtgacgg gggaggggga tttttgtgat tcaccaggca aaaagaaacc
    17641 ccgaagacag gcttcggggt taaagacgcg tatttattat catttttgca ctacgatttg
    17701 cgcatgctta acagtgcgcc gataaaaata cctaccgcag ctgctgtacc cacgctacac
    17761 cagggtcttt cacgaacaaa agaatccgcg cagccaacgg catcgcgtgc ggcttgctgg
    17821 acgcgagtac gaccatgcat tcgcgccctg gtttctttca gcaatgcctg agctttactg
    17881 cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg acttcaatac agattcgagg
    17941 ctgtcagcta attgattgac atcattctgg atatcctgca cgccatcatc aacgtcgttg
    18001 cggttcggtc ggttaaacat atgatccccc attagaattc gatgtaattt tcagtgtaga
    18061 ccataatttt cttaactgag gttcatcgcg gctttatgga cttttctgaa agcgttgtga
    18121 atgcgcaatc aggtaaggta ggcccgcggt tgataataac gaggatcaaa tatgtattta
    18181 cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga tgtggtaacc
    18241 cagaaagctt atggttaccg ccgtggggaa aattatgtct atgttaatcg cgaagcgcga
    18301 atgggacgta ccgcactggt aattcatcca acattaaaag aacgtagttc gacactggct
    18361 gaacccgctt ccgatattaa aacttgcgat cattatcaac aatttccgct ctatttagca
    18421 ggcgagcgac acgagcatta cggtatcccg catggcttca gttcgcgtgt tgcgcttgaa
    18481 cgttatttga atggtttatt tggcgaagcc agttaaagac gcgactggcg atgccagtcg
    18541 cgaaaagaag agtcaggctt ttacctgctg gtaatggctg actttaaaca gtcggcggca
    18601 ataatcgagg aagtagccat aaaccgcccc catcaacatc gaaacaacga tgtttgaact
    18661 gaccgccgcc ataatctgat gccagtctgc gcccaccact aacaagatcg ccacatacac
    18721 cggtgactgg aacgtcacat aagccaggat atcagccaga ttttttatcc agcccgacgg
    18781 gctaactttg cgtgccgcgc gcataaatag atcgcggtac ataccgtatg gccatgcaat
    18841 taagatgttc accggaatcg ctaccaatct ggaataaaaa gactgttcga agctcattcc
    18901 ggagaggaaa acttcaatac acatgttcac gacagaacag taaacaacca tcgcgaacgt
    18961 atctgcaact gcatgacgca agcgtgactg cggtgagaac atgctaatgc tccttaataa
    19021 aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc tcattgccat ctgcattttc
    19081 gcatagcgta tatcgctggc tatgactatt caattagtga aaaattttta tttagtcagt
    19141 ttttgtagat aaaatattcc gtaacggctc gttttttgta cattgtggat ttttacatgc
    19201 tgatatgatt tattttcaat taaatcaatt agatatgacc ttgcctttaa tttcactaat
    19261 gaaagtttgg gggtgaggta acgctataag cgtagagaat tcggtgtatg tcgtgaccgt
    19321 gtcatttaca gcatttttga tgccagaaaa tgtacattaa cagcagaaag caccagtg
//
LOCUS       DAJVXP010000076        18840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVXP010000076 DAJVXP010000000
VERSION     DAJVXP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.076"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="OQS10_002535"
     CDS             complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="OQS10_002535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS2293541.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="OQS10_002536"
     CDS             complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="OQS10_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS2293542.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2794..5148)
                     /gene="lon"
                     /locus_tag="OQS10_002537"
     CDS             complement(2794..5148)
                     /gene="lon"
                     /locus_tag="OQS10_002537"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS2293543.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="OQS10_002538"
     CDS             complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="OQS10_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS2293544.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="OQS10_002539"
     CDS             complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="OQS10_002539"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS2293545.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7605..8903)
                     /gene="tig"
                     /locus_tag="OQS10_002540"
     CDS             complement(7605..8903)
                     /gene="tig"
                     /locus_tag="OQS10_002540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS2293546.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="OQS10_002541"
     CDS             complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="OQS10_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS2293547.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9869..10447
                     /locus_tag="OQS10_002542"
     CDS             9869..10447
                     /locus_tag="OQS10_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2293548.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10491..11966
                     /gene="ampG"
                     /locus_tag="OQS10_002543"
     CDS             10491..11966
                     /gene="ampG"
                     /locus_tag="OQS10_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS2293549.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12427..13374
                     /gene="cyoA"
                     /locus_tag="OQS10_002544"
     CDS             12427..13374
                     /gene="cyoA"
                     /locus_tag="OQS10_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS2293550.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13396..15387
                     /gene="cyoB"
                     /locus_tag="OQS10_002545"
     CDS             13396..15387
                     /gene="cyoB"
                     /locus_tag="OQS10_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS2293551.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15377..15991
                     /locus_tag="OQS10_002546"
     CDS             15377..15991
                     /locus_tag="OQS10_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS2293552.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15991..16320
                     /locus_tag="OQS10_002547"
     CDS             15991..16320
                     /locus_tag="OQS10_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS2293553.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16332..17222
                     /gene="cyoE"
                     /locus_tag="OQS10_002548"
     CDS             16332..17222
                     /gene="cyoE"
                     /locus_tag="OQS10_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS2293554.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17407..17718
                     /locus_tag="OQS10_002549"
     CDS             17407..17718
                     /locus_tag="OQS10_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS2293555.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17702..18244
                     /locus_tag="OQS10_002550"
     CDS             17702..18244
                     /locus_tag="OQS10_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2293556.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18300..>18840)
                     /locus_tag="OQS10_002551"
     CDS             complement(18300..>18840)
                     /locus_tag="OQS10_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS2293557.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4952 c         4579 g         5093 t
ORIGIN      
        1 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
       61 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
      121 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
      181 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
      241 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
      301 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
      361 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
      421 cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
      481 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
      541 gccaaatttc agaccggcag cctgcatagc ttccgcccct ttgccggctt tcaaatcaac
      601 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
      661 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat
      721 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc
      781 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
      841 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
      901 ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg
      961 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
     1021 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
     1081 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
     1141 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
     1201 gataatatca gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
     1261 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
     1321 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
     1381 aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg
     1441 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
     1501 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
     1561 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc
     1621 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
     1681 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
     1741 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
     1801 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
     1861 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
     1921 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
     1981 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
     2041 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
     2101 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
     2161 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
     2221 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
     2281 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
     2341 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
     2401 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
     2461 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
     2521 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
     2581 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
     2641 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
     2701 atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
     2761 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
     2821 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
     2881 gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
     2941 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
     3001 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
     3061 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
     3121 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
     3181 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
     3241 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
     3301 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
     3361 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
     3421 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
     3481 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
     3541 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
     3601 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
     3661 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
     3721 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
     3781 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
     3841 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
     3901 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
     3961 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
     4021 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
     4081 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
     4141 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
     4201 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
     4261 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
     4321 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
     4381 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
     4441 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
     4501 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
     4561 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
     4621 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
     4681 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
     4741 gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag
     4801 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
     4861 ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat
     4921 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
     4981 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
     5041 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
     5101 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
     5161 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
     5221 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
     5281 aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt
     5341 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
     5401 caattaccga ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca
     5461 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
     5521 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
     5581 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
     5641 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
     5701 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
     5761 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
     5821 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
     5881 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
     5941 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac
     6001 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
     6061 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
     6121 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
     6181 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
     6241 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
     6301 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
     6361 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
     6421 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
     6481 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
     6541 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
     6601 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
     6661 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca
     6721 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
     6781 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
     6841 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
     6901 ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
     6961 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc
     7021 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
     7081 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
     7141 gatatctttt tctgggtttt ccgcttctag gaacagcatc tgcgccacaa tcaggttcgc
     7201 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
     7261 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
     7321 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
     7381 gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt
     7441 atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga
     7501 aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc
     7561 ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc
     7621 atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca
     7681 acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg
     7741 tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct
     7801 ttcacgcgct cttcgtcagc tttcagttcg ttggtgcgga taacttcgcc cagcagcagg
     7861 ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct
     7921 tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg
     7981 tcgatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga
     8041 gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca
     8101 gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc
     8161 agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta
     8221 cctttcagat tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg
     8281 ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc
     8341 gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg
     8401 aagtcgatgg ttacgcggtc ttctgcttca acggcgccgt ctttttcttt ccaggtcgcc
     8461 tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc
     8521 ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac
     8581 tctacagagt aggtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg
     8641 ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca
     8701 cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact
     8761 ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta
     8821 acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga
     8881 gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc
     8941 ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac
     9001 attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca
     9061 tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg
     9121 ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc
     9181 gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat
     9241 gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg
     9301 caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga
     9361 gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt
     9421 aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg
     9481 acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc
     9541 ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca
     9601 tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg
     9661 tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag
     9721 gcctcgcatc agcgtttctt ttaccagggg tgattatttc gccaacatga tgaatttaca
     9781 tgccttaccc acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac
     9841 acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc
     9901 tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat
     9961 tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga
    10021 tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac
    10081 cgcctcccga gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg
    10141 tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta
    10201 tgctgacgtg tcccagggca acgtgcgcta caacatcgcg accaaagcgg atatcgccat
    10261 catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt
    10321 tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac
    10381 cgataccatc gctgatatgt ctcaggacac cagcatccac gaattcatca agcagaacgc
    10441 gcgttaattt ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc
    10501 aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt
    10561 ccgggctacc gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata
    10621 ttgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc
    10681 tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc
    10741 tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg
    10801 gcacccaact ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc
    10861 aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg
    10921 gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc
    10981 tgtggctggc agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt
    11041 tgatcccttg tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc
    11101 caaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg
    11161 cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc
    11221 tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta
    11281 acaaaacgct tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc
    11341 agcgcctgtc gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg
    11401 ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct
    11461 ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcactg ttaatgacgc
    11521 tgtgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag
    11581 gacgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat
    11641 tctatctatt ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc
    11701 agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccgacag
    11761 gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac
    11821 tgctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg
    11881 aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg
    11941 ctctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg
    12001 ccgtaattat tacggctaaa taaacatcag tatcgcttaa taatatttgt tcttttgtgc
    12061 ggcttagcgt ttggaaatta ttgacgcaat ttatgaattc tattttttct tacacgattc
    12121 agctaatgag tctttatttt ccccccaccc aattgtcacc ataatgataa ttatttgtta
    12181 aacaattgtt ttattcaaga ttggttatac caattgcccg tccaaactcc ttctgcactc
    12241 ccctttcgtt ataacgccct tttgcaacag cttcttaaaa tcaacttgat atgttttgca
    12301 acatatgtaa cctgacagcc aaatccaagt aacaggaatt taatcatgtt tacagtaatg
    12361 taacctcccc gtaaaatgcc cacacacttt aaacgccacc agatcccgtg gaattgaggt
    12421 cgttaaatga gactcaggaa atacaataaa agtttgggat ggttgtcatt atttgcaggc
    12481 actgtattgc tcagtggctg taattctgcg ctgttagatc ccaaaggaca gattggtctg
    12541 gagcaacgtt cactgatact gacggcattt ggcctgatgt tgattgtcgt tattcccgca
    12601 atcttgatgg ctgttggttt cgcctggaag tatcgtgcga gcaataaaga tgctaagtac
    12661 agcccgaact ggtcacactc caataaagtg gaagctgtgg tctggacggt acctatctta
    12721 atcatcatct tccttgcggt actgacctgg aaaaccactc acgctcttga gcctagcaag
    12781 ccgctggcac acgacgagaa gcccattacc atcgaagtgg tttccatgga ctggaaatgg
    12841 ttcttcatct acccggaaca gggcattgca accgtgaatg aaatcgcttt cccggcgaac
    12901 actccggtgt acttcaaagt gacctccaac tccgtgatga actccttctt cattccgcgt
    12961 ctgggtagcc agatttatgc catggccggt atgcagactc gcctgcatct gatcgccaac
    13021 gaacccggta cttatgacgg tatctccgcc agctacagcg ggccgggctt ctcaggcatg
    13081 aagttcaaag ctattgcaac accggatcgc gccgaattcg accaatgggt cgcaaaagcg
    13141 aaacagtcgc cgaacaccat gtctgacatg gctgcgttcg aaaaactggc cgcgcctagc
    13201 gaatacaacc aggtggaata tttctccaac gtgaaaccag acttgtttgc cgatgtgatt
    13261 aacaagttta tggctcacgg taagagcatg gacatgaccc agccagaagg tgagcacagt
    13321 gcacacgaag gtatggaagg catggacatg agccacgcgg aatccgccca ttaaaggggt
    13381 tgaggaagaa taaagatgtt cggaaaatta tcacttgatg cagtcccgtt ccatgaacct
    13441 atcgtcatgg ttacgatcgc tggcattatt ttgggaggtc tggcgctcgt tggcctgatc
    13501 acttacttcg gtaagtggac ctacctgtgg aaagagtggc tgacctccgt cgaccataaa
    13561 cgcctcggta tcatgtatat catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac
    13621 gccattatga tgcgtagcca gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca
    13681 cctcaccact acgatcagat cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg
    13741 atgcctttcg ttatcggtct gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac
    13801 gttgcgttcc cgttcctcaa caacttaagc ttctggttta ctgttgttgg tgtgattctg
    13861 gttaacgttt ctctcggcgt gggcgaattt gcgcagaccg gctggctggc ctatccaccg
    13921 ctatcgggaa tagagtacag tccgggagtc ggtgtcgatt actggatatg gagtctccag
    13981 ctatccggta taggtacgac gcttaccggt atcaacttct tcgttaccat tctgaagatg
    14041 cgcgcaccgg gcatgaccat gttcaagatg ccagtattta cctgggcatc actgtgcgcg
    14101 aacgtactga ttattgcttc cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg
    14161 gatcgctatc tgggcaccca tttctttacc aacgatatgg gtggcaacat gatgatgtac
    14221 atcaacctga tttgggcctg gggccacccg gaagtttaca tcctgatcct gcctgttttc
    14281 ggtgtgttct ccgaaattgc ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg
    14341 ctggtatggg caaccgtctg tatcaccgtg ctgtcgttca tcgtttggct gcaccacttc
    14401 tttacgatgg gtgcgggcgc gaacgtaaac gccttctttg gtatcaccac aatgattatc
    14461 gccatcccga ccggggtgaa gatcttcaac tggctgttca ccatgtatca gggccgcatc
    14521 gtgttccatt ctgcgatgct gtggaccatc ggttttatcg tcactttctc ggtgggcggg
    14581 atgactggcg tgctgctggc agtaccgggc gcggacttcg ttctgcataa cagcctgttc
    14641 ctgattgcgc acttccataa cgttatcatc ggcggcgtgg tcttcggctg cttcgcaggg
    14701 atgacctact ggtggcctaa agcgttcggt ttcaaactga atgaaacctg gggtaaacgc
    14761 gcgttctggt tctggatcat cggcttcttc gttgccttta tgccgctgta tgcgctgggc
    14821 ttcatgggca tgacccgtcg tttgagccag cagattgacc cgcagttcca caccatgctg
    14881 atgattgcag ctagcggtgc agtactgatt gcgctgggta ttctctgcct cgttattcag
    14941 atgtacgttt ctattcgcga ccgcgaccag aaccgtgacc tgactggcga cccgtggggt
    15001 ggccgtacgc tggagtgggc aacctcttcc ccgcctccgt tctataactt tgccgttgtg
    15061 ccgcacgttc acgagcgtga tgcattctgg gaaatgaaag agaaaggcga agcgtataaa
    15121 aagcctgacc actatgaaga aattcatatg ccgaaaaaca gcggtgcagg tatcgtcatt
    15181 gcagctttct ccaccatctt cggtttcgcc atgatctggc atatctggtg gctggcgatt
    15241 gttggcttcg caggcatgat catcacctgg atcgtgaaaa gcttcgacga ggacgtggat
    15301 tactacgtgc cggtggcaga aatcgaaaaa ctggaaaacc agcatttcga tgagattact
    15361 aaggcagggc tgaaaaatgg caactgatac tttgacgcac gcgactgccc acgcgcacga
    15421 acacgggcac cacgatgcag gcggaaccaa aatcttcgga ttttggatct acctgatgag
    15481 cgactgcatt ctgttctcaa tcttgtttgc tacctatgcc gttctggtga acggcaccgc
    15541 aggcggcccg acaggtaagg acattttcga actgccgttc gttctggttg aaactttctt
    15601 gctgttgttc agctccatca cctacggcat ggcggctatc gccatgtaca aaaacaacaa
    15661 aagccaggtt atctcctggc tggcgttgac ctggttgttt ggtgccggat ttatcgggat
    15721 ggaaatctac gaattccatc acctgattgt taacggcatg ggtccggatc gcagcggctt
    15781 cctgtcagcg ttctttgcgc tggtcggcac gcacggtctg cacgtcactt ccggtcttat
    15841 ctggatggcg gtgctgatgg tacaaatcgc tcgtcgcggc ctgaccagca ctaaccgtac
    15901 ccgcatcatg tgcctgagcc tgttctggca cttcctggat gtggtttgga tctgtgtgtt
    15961 cactgttgtt tatctgatgg gggcgatgta atgagtcatt ctaccgatca cagcggcgcg
    16021 tcccatggca gcgtaaaaac ctacatgaca ggctttatcc tgtcgatcat tctgacggtg
    16081 attccgttct ggatggtgat gacaggagct gcctctccgg ccgtaattct gggaacaatc
    16141 ctggcaatgg cagtggtaca ggttctggtg catctggtgt gcttcctgca catgaatacc
    16201 aaatcagatg aaggctggaa catgacggcg tttgtcttca ccgtgctaat catcgctatc
    16261 ctggttgtag gctccatctg gattatgtgg aacctcaact acaacatgat gatgcactaa
    16321 gagcggcggt tatgatgttt aagcaatacc tgcaagtaac gaaaccaggc atcatctttg
    16381 gcaacctgat ctcggtgatt gggggattcc tgctggcctc aaagggcagc attgattatc
    16441 ccctgtttat ctacacgctg gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta
    16501 acaactacat cgacagggat atcgacagaa agatggaaag gacgaagaat cgggtgctgg
    16561 ttaaaggcct gatctctcct gctgtctcgc tggtgtacgc caccttgcta ggctttgctg
    16621 gctttatgct gctgtggttt ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct
    16681 ttgtggttta tgtcggcgtt tatagcctgt acatgaaacg ccactcggtc tacggcacat
    16741 tgattggttc gctctccggc gctgcgccgc cggtgattgg ctactgtgca gtaaccggcg
    16801 agttcgatag cggcgcagcg atcctgctgg ctatcttcag cctgtggcag atgcctcact
    16861 cctatgccat cgccattttc cgctttaagg attatcaggc ggcaaacatt ccggtattgc
    16921 cagtggtaaa aggcatttcg gtggcgaaga atcacatcac gctgtatatc atcgcctttg
    16981 ccgttgccac gctgatgctc tctcttggcg gttacgctgg gtataaatat ctggtggtcg
    17041 ccgcggcggt gagcgtctgg tggttaggta tggccctgcg cggttataaa gttgctgatg
    17101 acaggatctg ggcgcgcaag ctgttcggct tctctatcat cgccatcaca gccctctcgg
    17161 tgatgatgtc cgttgatttt atggtgccgg actcgcatac gctgctggct gctgtttggt
    17221 aacacaacct ctctattaaa aaggtgctac ggcacctttt ttattgtctg agatcagaga
    17281 tacggctaat aaccgtatgt cttgacagag tgagcagatg aacataaatt aatcatacgg
    17341 ttgatagccg tattatgtat gagatcgact ccctacgatt tcattcagtt aatcaaagag
    17401 gtcataatgc ctggaaaaac agccactcta cccgatgtcg ataaaacgct gaaaaatgcg
    17461 cgggtcgaac tcaaaactag tcccgacgcc aaaaacaaac ttcgtgaagc tgcgcaagcc
    17521 gttggggtag atttaagcgc ttttattttg agcgctgcta tggaacgcgc cgaaagtgtg
    17581 cttgataacc aacgtcgccg tgagctttcg aatcaaagct gggaactgat gaaccaactc
    17641 atcgctgaac ctgctcaacc gacgctcgcc ctcaaggcgt taatgaaaag gaaaaacagc
    17701 gatggtcgac aagcatgaag agattactct gcccatagtc ctctcctgta attatcagtc
    17761 tgatattact tatcctgggc aaaaacagtt tgattgcggt aaccctgtta tcgataaatt
    17821 tgtacgcgca tcgctaaaga aaagtgtgcg taatagcgac tgtgcggcta aagcacttat
    17881 tgacagacaa agtggtgaac tgatcggcat ctgtactttt acggcatatt cgctggaaaa
    17941 acaacgcgtt tctggcgtcc ttcagggttc acaaccttca gaaattggtg ttgtcagatt
    18001 agtcatgttg ggggtagcac ggaagtatca aaagcggggc tttggtcagg acctactatg
    18061 tgattttttt gaacatgtaa aaataattca ccaggcatta ccaattaaag gggtttatct
    18121 tgatgctgac cctgccgcca ttaattttta tgctcgtctc ggctttgttc agctttcagc
    18181 gacaccaaat gcttttggtg ctgtacctat gtttttggcg attcagcata ttctcgcggc
    18241 ttagattatt atcgccaccc gccagggcaa gttttgcctt agccgggggc tgagcagcgt
    18301 taccactttt gttcaattgc ccccgctttc agcaactcca catccttgcc gttaatggtt
    18361 gccgttgcgg caacgattgt ggtccttaca gttgcacaag attttcgctc aaattccggt
    18421 ttataaagaa taagagacga attactggta aatgaaccaa ataggtaatc agtaaagcta
    18481 atattcagcc ggtttaaacc gaaaggcgcg aagccaaccg tttggttaat ctccttaccg
    18541 gatgggcgac gaatgctgaa tgtcacgcga agattggcca cttcgcctaa tttattggtg
    18601 gtggcgaaga ggctatccat gcaggaaaaa cctttatcta aaaaattatc tcgcatccca
    18661 gaacgggtaa ccgtaatcac gggtaacgta tcgtcaaccg tttttctgtc aatcatgtct
    18721 tttcgacaag tagccagaaa gtcttccggg ccaccataaa tgtggacacc tttatcaaga
    18781 tattccttcg ctttcttaca atctacctca acatactgac cattggcgta cataatgccc
//
LOCUS       DAJVXP010000077        18821 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVXP010000077 DAJVXP010000000
VERSION     DAJVXP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.077"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..499)
                     /locus_tag="OQS10_002552"
     CDS             complement(<1..499)
                     /locus_tag="OQS10_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2293558.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMI"
     gene            695..1603
                     /locus_tag="OQS10_002553"
     CDS             695..1603
                     /locus_tag="OQS10_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS2293559.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1600..2862
                     /locus_tag="OQS10_002554"
     CDS             1600..2862
                     /locus_tag="OQS10_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS2293560.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            2859..3497
                     /locus_tag="OQS10_002555"
     CDS             2859..3497
                     /locus_tag="OQS10_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS2293561.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3502..4278
                     /locus_tag="OQS10_002556"
     CDS             3502..4278
                     /locus_tag="OQS10_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS2293562.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4367..5731
                     /locus_tag="OQS10_002557"
     CDS             4367..5731
                     /locus_tag="OQS10_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS2293563.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(5806..6795)
                     /gene="rpoS"
                     /locus_tag="OQS10_002558"
                     /pseudo
     CDS             complement(5806..6795)
                     /gene="rpoS"
                     /locus_tag="OQS10_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
     gene            complement(6858..7997)
                     /gene="nlpD"
                     /locus_tag="OQS10_002559"
     CDS             complement(6858..7997)
                     /gene="nlpD"
                     /locus_tag="OQS10_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS2293564.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8137..8763)
                     /gene="pcm"
                     /locus_tag="OQS10_002560"
     CDS             complement(8137..8763)
                     /gene="pcm"
                     /locus_tag="OQS10_002560"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS2293565.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8757..9518)
                     /gene="surE"
                     /locus_tag="OQS10_002561"
     CDS             complement(8757..9518)
                     /gene="surE"
                     /locus_tag="OQS10_002561"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS2293566.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(9499..10548)
                     /gene="truD"
                     /locus_tag="OQS10_002562"
     CDS             complement(9499..10548)
                     /gene="truD"
                     /locus_tag="OQS10_002562"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS2293567.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(10545..11024)
                     /gene="ispF"
                     /locus_tag="OQS10_002563"
     CDS             complement(10545..11024)
                     /gene="ispF"
                     /locus_tag="OQS10_002563"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS2293568.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11024..11734)
                     /gene="ispD"
                     /locus_tag="OQS10_002564"
     CDS             complement(11024..11734)
                     /gene="ispD"
                     /locus_tag="OQS10_002564"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS2293569.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11753..12064)
                     /gene="ftsB"
                     /locus_tag="OQS10_002565"
     CDS             complement(11753..12064)
                     /gene="ftsB"
                     /locus_tag="OQS10_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS2293570.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12258..12581)
                     /locus_tag="OQS10_002566"
     CDS             complement(12258..12581)
                     /locus_tag="OQS10_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS2293571.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(12631..13236)
                     /gene="cysC"
                     /locus_tag="OQS10_002567"
     CDS             complement(12631..13236)
                     /gene="cysC"
                     /locus_tag="OQS10_002567"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS2293572.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13236..14663)
                     /gene="cysN"
                     /locus_tag="OQS10_002568"
     CDS             complement(13236..14663)
                     /gene="cysN"
                     /locus_tag="OQS10_002568"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS2293573.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(14665..15573)
                     /gene="cysD"
                     /locus_tag="OQS10_002569"
     CDS             complement(14665..15573)
                     /gene="cysD"
                     /locus_tag="OQS10_002569"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS2293574.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15825..16862
                     /gene="iap"
                     /locus_tag="OQS10_002570"
     CDS             15825..16862
                     /gene="iap"
                     /locus_tag="OQS10_002570"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS2293575.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17130..17423)
                     /gene="cas2"
                     /locus_tag="OQS10_002571"
     CDS             complement(17130..17423)
                     /gene="cas2"
                     /locus_tag="OQS10_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS2293576.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(17420..18343)
                     /gene="cas1e"
                     /locus_tag="OQS10_002572"
                     /pseudo
     CDS             complement(17420..18343)
                     /gene="cas1e"
                     /locus_tag="OQS10_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18340..>18821)
                     /gene="cas6e"
                     /locus_tag="OQS10_002573"
     CDS             complement(18340..>18821)
                     /gene="cas6e"
                     /locus_tag="OQS10_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS2293577.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4430 a         5087 c         4721 g         4583 t
ORIGIN      
        1 caatcatcag gctgcgcagc atggtcgcag cggcttcccc gacacaggaa cggttttccc
       61 gacacactgc accgccagtg tgaataattg tgcaattact gttgtcgagc aagtagttcg
      121 caataacgaa atcgtttgtg accacagtca gtgactccat gtgaatcaga tgctgtgcta
      181 tcgctaacgt ggtcgttccc gcatccagat agatacaact tcccggctga acaagacttg
      241 ccgccagctt gccaatagcc gctttttgcg tcattgccag cgcagttttt acctgatgag
      301 aaggttcatg cgccacgcgt cccggagact ggacgcctcc ggacaccagc acaacggctc
      361 cctgctgctc cagtttttgt aaatcccgac gaatggtcat atgtgacaca ttcattctgt
      421 ccgttagttc agcaatactg acaatgcctt tttcagctac catctcaagg atgatttggc
      481 gacgctctac gggtatcaac ttttgctcct tcctttgtcc tgctgacatt ctacgctatt
      541 tgcctgcgaa acgtgcgcgg cgcaactaac gcttagttca cataaaataa cacacaatgt
      601 taatttatgt gaatcagctc accatatcgt tatcttccag cgcttatatt cacaatatca
      661 aacaaaatat cacttaaatt aacaatgaga gcagatgaaa acgggatctg agtttcatgt
      721 cggtatcgtt ggcttagggt caatgggaat gggagcagca ctgtcatgtg tccgcgcagg
      781 tctttctacc tggggcgcag acctgaacag caatgcctgc gctacgttga aagaggcagg
      841 tgcttgcggg gtttctgata acgccgcgac gtttgccgaa aaactggacg ctctgctggt
      901 gctggtggcc aatgcggccc aggttaaaca ggtgctgttt ggtgaaacag gcgttgcaca
      961 acatctgaaa cccggtacgg cagtaatggt ttcttccact atcgctagtg ctgatgcgca
     1021 agaaattgct accgctctgg ctggattcga tctggaaatg ctggatgcgc cagtttctgg
     1081 tggtgcagta aaagccgcta acggtgaaat gactgtcatg gcctccggta gcgatattgc
     1141 ctttgaacga ctggcacccg tgctggaagc cgttgccgga aaagtttatc gcataggtgc
     1201 agaaccggga ctaggttcga ccgtaaaaat tattcaccag ttgttagcgg gcgtacatat
     1261 tgctgccgga gccgaagcga tggcacttgc agcccgtgcg gggatcccgc tggatgtgat
     1321 gtatgacgtc gtgaccaatg ccgccggaaa ttcctggatg ttcgaaaacc ggatgcgtca
     1381 tgtggtggat ggcgattaca ccccgcattc agccgtcgat atttttgtta aggatcttgg
     1441 tctggttgcc gatacagcca aagccctgca cttcccgctg ccattggcct caacagcatt
     1501 gaatatgttc accagcgcca gtaacgcggg ttacgggaaa gaagacgata gcgcagttat
     1561 caagattttc tctggcatca ctctaccggg agcgaaatca tgatcaagat tggcgttatc
     1621 gccgatgatt ttaccggcgc gacggatatc gccagttttc tggtggaaaa cggtctacca
     1681 acggtacaaa ttaacggtgt tccaacaggt aaaatgccgg aagcaatcga cgcactggtg
     1741 atcagcctga aaacgcgctc ctgtccggtg gttgaagcca cacagcaatc gctggcggct
     1801 ctgagctggt tgcaacagca aggttgcaaa cagatctatt tcaaatactg ctctactttc
     1861 gacagtacgg cgaaaggtaa tattggcccg gttaccgatg ccttaatgga tgctctcgac
     1921 acgccgttta cggtcttctc tccggccctg tcggtcaacg gacgtacggt ttatcagggg
     1981 tatttgttcg taatgaatca actgctggcc gaatccggga tgcgccatca cccggtaaat
     2041 cccatgaccg acagctatct tccccgtctg gttgaagcgc aatccacagg gcgctgcggc
     2101 gtcgtttcgg cacatgtttt cgaacaaggc gtggatgccg ttcgtcaaga gctggctcgc
     2161 ttacagcaag agggctaccg ctacgcggtg cttgatgcgc tgaccgaaca ccatctggaa
     2221 attcagggag aagccttgcg cgatgcccca ctggtaacgg gcggttctgg tctggcgatt
     2281 ggcctggccc ggcagtgggc gcaagaaaac ggtaaccagg ctcgcgaagc agggcgtccg
     2341 ctcgctgggc gcggcgtagt gctctccggt tcatgctctc aaatgaccaa ccgccaggtg
     2401 gcacattacc gtcaaattgc accagcccgt gaagttgatg tggcacgctg cctctcaacc
     2461 gaaactctgg ccgcttatgc acacgaactg gcagagtggg ttctgggcca ggaaagtgta
     2521 cttgctccac tggtttttgc caccgccagc actgacgcat tggcagcaat tcaacagcaa
     2581 tacggtgcac aaaaagccag tcaggcagta gaaactctgt tttctcaact agcggcgcgg
     2641 ttagcagcgg aaggcgtgac acgctttatt gtcgcaggcg gtgagacctc cggcgtagtc
     2701 acacagagcc tgggcataaa agggtttcat attggcccaa ccatttcccc cggcgtgccg
     2761 tgggtaaacg cactggataa gcctgtctca ctcgccctta aatctggcaa cttcggtgat
     2821 gaagcctttt tttcacgagc ccaaagagag tttttatcat gagcgatttc gcaaaagtag
     2881 agcagtcttt gcgagaggag atgacgcgga ttgccagttc attctttcag cgcggctatg
     2941 caaccggttc ggctggcaat ctgtcgctgc ttttacctga cgggaattta ctggcgacac
     3001 cgacaggttc atgcctgggc aatctcgatc cgcagcggct ttccaaagtc gccgcggatg
     3061 gcgaatggtt aagtggtgac aaaccctcga aagaggtgct ctttcatctg gcgctgtatc
     3121 gcaacaatcc gcgctgtaaa gcggtggtgc atttgcacag cacatggtcg acggcgcttt
     3181 cctgcctgca agggctggac agcagcaacg ttattcgtcc gttcacacca tacgtggtga
     3241 tgcggatggg aaatgtcccg ctggtgcctt attaccgacc gggcgataaa cgcatcgcac
     3301 aggatctggc ggaactggca gcagacaatc aggctttttt actggcaaat catggcccag
     3361 tggtttgcgg tgaaagcctg caagaagccg ccaacaatat ggaagagctg gaggaaacgg
     3421 caaagctgat ttttattctc ggtgaccgcc cgatccgtta tctgaccgca ggtgaaattg
     3481 cggaattaag gagttaatgc aatgcctcgt tttgcagcta atttatccat gatgttcacc
     3541 gaagtgcctt ttattgaacg cttcgccgca gcgcgaaaag ccgggttcga tgctgtggaa
     3601 tttctgtttc cctatgacta ctccaccctg caaatccaaa agcaactgga gcaaaaccat
     3661 ctgacactgg cgctgttcaa taccgctccc ggagatatta atgcggggga atggggatta
     3721 tccgcccttc ccggacgtga gcacgaagca cacgcagaca tcgacctggc actggaatat
     3781 gcattggcgc tcaattgtga acaagtccat gtgatggcag gcgtcgtgcc cgctggcgaa
     3841 gacgctgaac ggtaccgggc agtatttatc gataatctcc gctacgctgc cgaccgcttt
     3901 gcgctacacg gcaagcgaat attagttgaa gcactcagcc ccgacgttaa gcctcattat
     3961 ctcttctcca gccagtatca ggcactggct attgttgaag aggttgcgcg agataacgtg
     4021 tttattcaac tagatacttt tcatgcgcaa aaagtagatg gcaacctgac gcatttaatc
     4081 cgcgactacg ctgggaaata tgcgcacgta caaattgccg gactacctga tcgacatgaa
     4141 ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga ggtgggatat
     4201 cagggctgga tcggttatga atataaacct cgtggcctca ccgaagaagg gcttggctgg
     4261 tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa cgtacccata catcccccca
     4321 tctacggatg gggtaggtta actataactt tcagacaggg tttcccatgt ccacaattac
     4381 attgttatgc attgcgttag ctggcgtaat catgctgttg ctgctggtca tcaaggcaaa
     4441 ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg cggcgggtat
     4501 accggcgggc gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc ttggttccgt
     4561 cactattatt attggtctgg gcgctatgct ggggcgtatg atcgaacact ctggtggtgc
     4621 agagtcactg gctaattatt tcagtcgcaa gttaggtgac aaacgaacta tcgctgcgct
     4681 gactctggca gcgttcttcc tcggtattcc cgtcttcttt gatgtcggct ttattattct
     4741 tgctccaatc atttacggtt ttgccaaggt tgccaaaata tcgccactca aatttggcct
     4801 gcctgtcgct gggatcatgc taactgtaca tgtggcggta ccgccgcatc caggccctgt
     4861 cgccgcagcg gggttactcc acgcagacat cggctggcta accatcatcg gtattgcgat
     4921 ttctattccc gtagggattg ttggctactt tgcagcgaaa ataatcaata agcaccaata
     4981 tgcgatgtcg gtggaagtac tggaacagat gcaactggct ccggccagtg aggaaggcgc
     5041 gacaaaatta agcgataaaa taaatccacc gggcgtcgcg ctggtcacct cgctaattgt
     5101 tattcctatc gcgattatca tggcgggtac ggtttccgca acactgatgc cgccttcgca
     5161 tcccctgctt ggtacgctac agctgatcgg ctcaccaatg gtagctctaa tgattgcgct
     5221 ggtgctggca ttctggttat tggctttacg tcgcggctgg agcttacaac acaccagcga
     5281 cattatgggc tcagcccttc ccactgcggc ggtagtgatt ttggttactg gtgctggagg
     5341 ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca acatgctaca
     5401 aatgattgac ctgcctctgt taccagccgc atttattatt tcactggcgc tgtgtgcatc
     5461 gcaggggtca gcaaccgtag caatcctgac aaccggcggg ttactctcag aagcggtgat
     5521 gggattgaat ccgattcagt gcgtattggt gacgctggca gcctgctttg gtgggcttgg
     5581 ggcgtcacat attaatgact cagggttctg gattgtgacc aaatatctgg ggttgtcggt
     5641 agcagacggt ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta ccggattctt
     5701 aattacctgg tgcgtatggg cggtaatttg accttaataa aaaggtccga tgagcatcgg
     5761 accttttatt gtgcacagaa aaggccagcc tcgcttgaga ctggcctttc tgacaggcgc
     5821 ttcgatattc agcccctgcg tttgcaggat ttcacgcaaa cggcgcaggc cttcaacctg
     5881 aatctggcga acacgttcac gggtgaggcc aatttcacga cctacatctt ccagtgttgc
     5941 cgcttcgtac cccagcaaac cgaatcgacg tgccagtact tcacgctgtt tggcgttcag
     6001 ctcgaacagc catttgacga tgctctgctt catatcgtca tcttgcgtgg tatcttccgg
     6061 accgttctct ttttcatcgg ccaggatgtc cagcaacgct ttttcggaat caccacccag
     6121 cggggtgtct accgaggtaa tgcgctcgtt aagacgaagc atacggctga cgtcatcaac
     6181 tggcttatcc agttgctctg cgatctcttc cgcacttggt tcatggtcca gcttatggga
     6241 caactcacgt gcggttcgca ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg
     6301 aatagtacgg gtttggttca taatcgcccg ttcaatcgtc tggcgaatcc accaagttgc
     6361 gtatgttgag aagcggaaac cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc
     6421 caggttgccc tcttcgataa ggtccagcaa cgccagacca cgattgccat aacggcgggc
     6481 aatttttacc accagacgca agttactctc gatcatccgg cggcgagagg cgacatctcc
     6541 acgcagtgcg cgacgcgcaa aataaacttc ttcttcggcc gttaacagtg gtgaataacc
     6601 aatctcacca aggtaaagct gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa
     6661 cagttcctct tcggccaaat cgttatcact gggttcctct tctactaagg ccttttcgtc
     6721 aaaaacctca actccgttct catcaaattc cgcatcttca tttaaatcat gaactttcag
     6781 cgtattctga ctcataaggt ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa
     6841 agcctggttc cgccgattta tcgctgcggc aaataacgca gcgggtttac ggatttcccc
     6901 ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc cggtgctacc catggttgct
     6961 attttttgcc ccgccttcac ttcttgttgt tcccggacca gcattgtgtc gttatgggcg
     7021 taggcactca ggtaatcatc attatgtttg atgataatca gattaccgta gccgcgcagc
     7081 gcgttaccgg cataaacaac gcggccatct gcggtcgcga taattgcctg tcctttgctg
     7141 cctgcgatat caatcccctt gttgcccccc tcagaagcgc caaaggtttc gatcactttg
     7201 ccctcagtcg gccagcgcca ggtggagata ggcgtactgg ttgatgtact gctgacagtc
     7261 ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg tgactgtggt cgcagttggc
     7321 ttgttgttcg gcaacatttt gttagcactc tgttcacccg aagactcaga atacgtaatt
     7381 gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag gcttgatcac aactccttgc
     7441 tctgctgcgt cggcctgggt aatggcattt ccgccagtga ttggcgtacc ggaagcatta
     7501 cccacctgca aggtctgacc aacgttcagc gcgtatggtg cctgaatatt gttgcgctga
     7561 gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat agaaaagtgt gtcgcctttt
     7621 ttcacggtat aggtactgcc gctataactg cctttcggaa tgttcccata ctgacggtta
     7681 tagacgatgc gtccgttttc catctgtacc ggctgctgag ctactggctg cactggctgg
     7741 atttgcggtt gttgagtagc ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc
     7801 tgggctgtag acgtcgtccc cattttcggc ggcggcgtaa tcaacatacc agaattagta
     7861 tttgcaggcg cattgccatt aacggagctg accggtgccg gtggatttga agtgtcagaa
     7921 cagcctgcca gccatagcga aaccagtgac aaagcagcaa tgcggcgaac ggtgaatttt
     7981 gggcttcccg cgctcattta tcccccagga aaaattggtt aataaccagt gacataatta
     8041 ccgtgcaagg caccctactg aacactggaa aagatgttca cgatacgctg acctgcggca
     8101 aaataaccag gaaaaatcca ggtatttcct cacgttttaa gccagctcac ccttcactaa
     8161 agggacaaag cgcacggcct ccacggtatc gataataaat tcgcctcccc gacgacgcac
     8221 ccgtttcaaa tactggtgct cctcccctac gggtaagacg agaatcccgc cttcgtccag
     8281 ctgcgtcatt agcgcagttg gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa
     8341 cggcgcacgt gcctgccaac cttgccatcc atcgccatga cgggttgaaa cattatgtaa
     8401 atcaagattt ttcaggcggc gacgtgcctg ccactgcaag cctttaatcc gttcaaccga
     8461 gcaaacatgc tggacaagat gcgctaggat tgccgtttga tatcccgaac cagtgccaat
     8521 ttccagcacc cgcgactgcg gcgtcagctc gagtaattcg gtcattcgcg ccaccatata
     8581 cggctgcgaa attgtctgcc cctgacctat cggcaaggcg atattgtccc aggctttttg
     8641 ttcaaacgct tcatcaacga atttttcacg cggcacggcg gcaagtgcat tcagcacctg
     8701 ctcatcctga ataccttgcg cacgtaattg ctccagaagt gcttgtacgc gtctgcttac
     8761 cattgcgtgc caactcccac gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc
     8821 gcagttaaat ccacatgcag cggcgtgatg gagacatagc cctcatctac cgcagcaaaa
     8881 tcggtccccg gaccggcatc acatttaccg cccggcgggc caatccagta cagcgtattg
     8941 ccgcgcggat cttgctgcgg gatcacctga tctgccggat gtcgtgtacc gcagcgcgtc
     9001 acgcgaatac ctttgatttg atccaagggt aaatccggaa cgttaatatt aagaatacgt
     9061 ccggtgcgca gcggctcttt acacagtgcg cgcaaaattg aacaggttac cgccgcggca
     9121 gtgtcgtaat gtttatgccc gtcaagcgag acggccagcg ccggaaaacc taaatgacgg
     9181 ccttccatcg cggcggctac cgtaccggaa taaataacgt catcccccag attcggcccg
     9241 gcgttaatgc cggacacaac aatgtccggg cgcggacgca tcagagcatt cacgccaaga
     9301 tagacgcaat cggtcggggt tcccatttgc acagcaatat caccattttc aaaggtaaac
     9361 gtgcgcaggg aggattccag tgtcagagaa tttgaagcgc cgctgcggtt acgatcgggg
     9421 gcgaccacct gaacgtcagc aaactcacgc aaggctttcg ccagcgtttg tataccgggt
     9481 gcatgtaccc cgtcatcatt actcagcaat atgcgcataa tcacctgttg tgttgataag
     9541 ttccctgaca acgctggttg caaaactacc cgccggaagc cagaaacgga tctctacggt
     9601 gacgtcatcc caccaattcc agcttaattg ttgcggatac agcaacatcg ctctgcgcgc
     9661 ggcttcaact ttttcgcgca ccagtaaagc ttgtaattca gtttctgcgg cgacagctgc
     9721 cttttcgaat gccagcgctt cacgctgagt tccccattcg ccactacccg gcaatgcggc
     9781 ggttatcatc agctctttat cgttgacgcg acgctgtaat tccgccagtt cttcggtggt
     9841 tgcgacaaac cagctaccac gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt
     9901 aacgtctgct tttttgaggc gctcagcaac aatctgatta aacaacgcac tgcgggctgc
     9961 cgacaaccaa aaactccgtt tattgcgatc gcgcaccgga gtattggttt gcgcccagcg
    10021 ctgcgccccc tgcaagttgc taccgccaat cccaaaacgt tgggcaccga agtagttcgg
    10081 tacacctttt acgcaaatat cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc
    10141 gcgcagaacc agggtaaagg cgttaccttt cagcgcgcct aaacgcagct tgcgcttgtg
    10201 ccgcgcatac tccagcacct ggcagccttc cagttgaaag gcgctcagat cgggcatttc
    10261 cttgcctggc acgcgagcgc ataaccactg ttccgtaaca gcatgtttgt ctttttgccc
    10321 agcgaagctg acttcacggg catgaatttt caggaatttc gccagtgcat ccgccacaaa
    10381 acgggtattg cagccgtttt tgaggattct aaccagaata tgctcacctt caccatcagg
    10441 ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga ttggctttca gcagcccggt
    10501 gccttgcggt ttaccgtgga ggtaagtgag attatcaaac tcaatcattt tgttgcctta
    10561 atgagtagcg ccaccgcttc acaggcaatc ccttccccac gtccggtaaa tccaagtttt
    10621 tccgtagtag tggctttcac gttaacatca tccatatggc agccgagatc ttcggcaata
    10681 aacacgcgca tttgtggaat gtgcggcaac atcttcggtg cctgagcgat aatagtgaca
    10741 tcgacgttgc ccagggtata acccttcgcc tgaatacgac gccaggcttc gcgtagcagc
    10801 tcgcggctgt cggcaccttt aaatgccgga tcggtatccg ggaacagctt gccgatatcc
    10861 cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat ggagcgccac gtcgccatca
    10921 gaatgcgcca gcaatccttt ttcgtaagga atgcgtacgc caccaatgat aattgggcct
    10981 tcaccgccaa aggcatgtac gtcaaaaccg tgtccaattc gcattatgta ttctcctgat
    11041 ggatggttcg ggtgaggtaa aactcggcca gtgccaaatc ttccgggcgc gtgactttaa
    11101 tgttatccgc acggccttcg accaactgag gatggaatcc gcaatattcc agcgccgagg
    11161 cttcgtcggt aatagtcgcg ccttcattta gagcgcgcgt cagacagtca tgtaacagct
    11221 cacgagggaa aaattgcggc gtcagcgcgt gccataagcc gttgcgatca acggtatgag
    11281 caatggcatt tttgcccggt tcggcacgtt tcatcgtatc gcgcactggt gcggctagga
    11341 tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca
    11401 gacaaggacg agcggcgtca tgcaccaata cccactgcgc gtcgccagcg gctttcaaac
    11461 ctgccagcac ggaatcggca cgctcatcac cgccatctac aacggtgatt tgcggatgat
    11521 tcgccagagg aagttgtgca aaacggctat cgccaggact tatggcaatg acgacacgtt
    11581 tcacccgggg atgcgccagc agcgcatgca ccgagtgttc aagaatggtt tgattaccga
    11641 ttgagagata ttgcttagga cattccgttt gcattcgacg gccaaatccg gccgccggaa
    11701 ccacggcgca aacatccaaa tgagtggttg ccatgttaat tcccgggctg atttatcgat
    11761 tgttttgccc cgcagactgt gcgcgcttcg acgcgtcagg caccagacga taaaaagttt
    11821 cgcccggcct ggtcatgctg agttcattac gcgcacgctc ttcgagcgcc tcctggccgc
    11881 cattgagatc gtcaatttcg gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg
    11941 tagcttgctg tgccgccaca tcatcattga cgcgggtata gtcatgtata ccgttcttac
    12001 cgaaccacag cgaatactgt agccagacca gaatagccag caacagcagc gttagtttac
    12061 ccatcctgcc ccctgaaaaa cggcatcatc atcccatgca tccgaagacg actctacatc
    12121 ctctgttggg gataccgcga caacgcgggc aaatgtacca catttgtcca ttgttacgta
    12181 tacccagggc gtgcagaaca taatctcatt attagttacg gtttgaatta tgaacagagg
    12241 agacaagaaa gtacaaatta gcccagtagc cacataaaca gtgcgccaaa cataatgcct
    12301 actgtcacca gggtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagc
    12361 gcaatcccca ccactaccgc gacgggcatc agcgccagaa agaaaggcca ggtgtagata
    12421 aagaagaaca gcgtgttaga gccataaatc aacatcggca tcgccagcgc aaataaccag
    12481 gagataaaac cgaccacggc accaggcagt gaccatgtgg tttcttcatc ctcagtaagg
    12541 ctgtcgttat ttgttagtgt aatgttatgg ctattacgca tatttgatcc tgttactttg
    12601 acgaaccggg catggaaacc cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa
    12661 cagatctaat aattgctgta ccaaatttgt tactaattgt tcaccattga gatgaatttc
    12721 tgccgattca ggcgcttcgt aaacggaatc tattcccgta aagttgcgca gttcaccggc
    12781 acgcgctttc ttatataagc cttttggatc gcgggcttcg caaatcgcca gcggcgtatc
    12841 gacaaacact tcgataaagc gcccttctcc tacgcgttca cgaaccatct ggcgttcggc
    12901 gcggtgtggc gagataaatg cggtcagcac caccagtccg gcttcaacca tcaaattcgc
    12961 cacttcaccg acgcgacgga tattctcttt acgatcggca tcgctaaaac cgagatcgct
    13021 gcataatccg tggcgaacat tatcgccatc cagcagatac gtactgacgc cgagtttatg
    13081 taacgcctcc tccagcgccc cggcgaccgt tgatttaccg gacccggaga ggccggtaaa
    13141 ccacagcact acaccacgat gaccgtggtg tagctcgcgt tgttgcacag tgaccggatg
    13201 gctatgccag acgacgtttt cgtcatgcag cgccattatt tatcccccag caaatcgcgc
    13261 gcgccccagt gcggaaagtg gcgacgaacc agagcattca attccagttc gaatgcactg
    13321 aattcagatg gcgcagcagt tgcctggcta actggctcgt gcaccatacc ggcacccacg
    13381 gtcacattgc tcaggcgatc gataaaaatc agcccacccg tcaccggatt ttgttgataa
    13441 cgatctaaca ccagcggctc gtcaaaagtg agatccacga ggccgatccc attcagtggc
    13501 aggttttcaa cttcacgctg ggtaaggtta ttaatatcaa cctgatagcg aatgccatca
    13561 acacgcgcgc gcgtcttctt accggcaatt ttgatgtcgt aactctgccc tggagaaagc
    13621 ggctgttccg ccatccatac cacatccacc gacgcgctct gcaccgccgg taacgcttcg
    13681 tctgccgcca gcagcagatc gccacggctg atgtcgatct catccgtcag caccagggtg
    13741 atcgcttctc cggcaaaggc ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg
    13801 tttgattcca caccagaggg cagcactttt acacgttgcc cgacttccac gcgaccggat
    13861 gccagcgttc cggcgtagcc acgaaaatcg agattcgggc gattaacgta ctgcaccggg
    13921 aagcgcattg gctgagcatc caccactcgc tggatctcca cggtttccag cacttcgagc
    13981 agtgtcggac cgctgtacca cggcatactt tcactttgcg atgccacgtt gtcgccttcc
    14041 agtgcagaga gcggcacaaa gcggatatcc agattacccg gcagctgccc ggcaaaggtc
    14101 agataatctt cacgaatacg ggtgaacgtc tcttcactgt aatccaccag atccattttg
    14161 ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg agataaaact gtgacgacgg
    14221 gtttgatcga gcacgccttt acgggcatcg atcagtaaga tcgccagttc acatgtcgat
    14281 gcgccagtcg ccatattgcg agtgtactgc tcgtgccctg gggtgtcggc gataataaat
    14341 ttacgcttct cggtagagaa atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc
    14401 tcagcttgca ggccgtccac cagcagagcc agatccagct tttcgccctg ggtaccgtga
    14461 cgcttactgt cgttatgcag cgatgagagc tgatcttcat agatttggcg agtatcgtgc
    14521 agcaggcgac caatcagagt gcttttgccg tcatcgacgc tgccacaggt cagaaaacgc
    14581 agcaggcttt tatgttgttg cgcaatcatc caggcttcga cgccgccttc attggcgatt
    14641 tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga cgttttttca gctccataga
    14701 tcccgcctgg tcgcggtcaa tcacgcggcc ctgacgttca ctggtggtgg aaaccagcat
    14761 ctcttcgatg atttccggca gtgtttgtgc atttgactcc accgcaccgg tcagcggcca
    14821 gcagcccagc gtacggaaac gcaccatccg ttttttaatc acttcgcccg gttgcaggtc
    14881 gatacggttg tcatcaatca tcatcaacat accgtcgcgt tccagaaccg gacgttccgc
    14941 agcgagatac agcggcacaa tgtcgatatt ttccagccag atgtattgcc agatatcctg
    15001 ctcggtccag ttagagagtg ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt
    15061 gtagttgtgc cacagctccg ggcgctgatt tttcggatcc cagcgatgga agcggtcacg
    15121 gaaagagtag attcgctctt tagcgcggga tttctcttcg tcacggcgcg caccaccgaa
    15181 ggcggcatca aaaccgtatt tgttcagcgc ctgtttcagg ccttcagttt tcataatatc
    15241 ggtatgtttc gcgctgccgt gcacgaatgg attaatcccc atcgccacgc cttccgggtt
    15301 tttatgcacc agcagctcgc agccgtaggc tttcgccgta cgatcgcgga actcatacat
    15361 ctcgcggaat ttccagccgg tatcgacatg cagcaacggg aaaggcagcg tacctggata
    15421 aaacgccttg cgcgccaaat gcagcatgac gctggaatct ttaccgatag agtagagcat
    15481 caccggattt gagaattctg ccgccacctc gcgaataatg tggatgcttt ccgcctccag
    15541 ttgccgcagg tgagtaagtc gtatttgatc cataaccgtt cctttgcaat accgctattt
    15601 tcttgccatc agatgtttcg actataggga gcgtaagaga acgaatgaaa ttaccaatta
    15661 gaatgagtag ttccttaacg gaataacgat ttggcaaagc taatatcaaa aagtgcttaa
    15721 ggcaccggat ttctggcgtt taggaagatt tgaaattgtt ttagcgcagc ggaagtttca
    15781 tactatggcg gtaaaaaaat ttgcatggta tttaaggact cactatgttt tccgcattgc
    15841 gccaccgtac cgctgccctg gcgctcggcg tatgctttat tctccccgta cacgcctcgt
    15901 cacctaaacc tggcgatttt gctaatactc aggcacgaca tattgctact ttctttccgg
    15961 gacgcatgac cggaactcct gcagaaatgt tatctgccga ttatattcgc caacagtttc
    16021 agcaaatggg ttatcgcagt gatattcgga catttaatag tcggtatatt tatactgccc
    16081 gcgataatcg taagagctgg cataacgtga cgggaagtac ggtgattgcc gctcatgaag
    16141 gcaaagcgcc gcagcagatc atcattatgg cgcatctgga tacttacgcc ccgctgagcg
    16201 atgctgacgc cgatgccaat ctcggcgggc tgacgttaca aggaatggat gataacgccg
    16261 caggtttagg tgtcatgctg gaactggcag aacgcctgaa aaatacgcct accgagtatg
    16321 gtattcgatt tgtggcgacc agcggcgaag aggaaggaaa attaggcgct gagaatttac
    16381 tcaagcggat gagtgacacc gaaaagaaaa atacgctgct ggtgattaat ctcgataact
    16441 taattgttgg cgataaattg tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga
    16501 aattaacgcg cgacagggcg ctggcaattg cgcgcagtca cggaatagcc gcaacgacca
    16561 atccgggttt gaataaaaat tatccgaaag gcactgggtg ttgtaatgac gcagaaatat
    16621 tcgacaaagc gggcattgct gtactttcgg tggaagcgac taactggaat cttgggaata
    16681 aggatggtta tcagcaacgc gcaaaaacag ccgcattccc tgcgggaaat agctggcatg
    16741 acgtaagact ggataatcaa cagcatattg ataaggcact tcctggaaga atagaacgtc
    16801 gctgccgtga cgttatgcgg ataatgctac ctctggtgaa ggagttggcg aaggcgtctt
    16861 gatgggtttg aaaatgggag ctgggtgttc taccgcaggg gcggggaatt ctaagtgata
    16921 tccatcatcg catccagtgc gcccggttta tccccgctgg cgcggggaac tcttactgct
    16981 tggtatgcgg aatcacaccc tgaacggttt atccccgctg gcgcggggaa cacactaagc
    17041 atacataact gtttttaaac aaatttattc cgcatcaaca atctaccaac taaattaaaa
    17101 catttcctta tttttaaaga acggataact tattgattat caacaggaag aaaagaaacc
    17161 aaacgtaacc catccaaatc caccggaata cgtctgtttt ctccccaggt ctgaaattca
    17221 aaacccgact cgatattggt cgcccaggcc atcaccacat ttccgcaacc agccagttgg
    17281 gtaatttgct gccagatcat ctcccgaata cgttttgatg tatcaccaac atacacaccg
    17341 gcacgcactt ccagtagcca gattgcgagc cgtccacgta agcgcggagg gacattttct
    17401 gtaacaacca cgaccatgct catccgccgc gcccccggtg accactatca cccagcgttt
    17461 caggttcagg gatggcaggc ggtaacatat ccggcgcggg ttgtggtggt tcaatttcac
    17521 ctgcagcaag gacttcctca attaacggta ttaatttgcc cgttaactta gtgctacgga
    17581 aaatatcgcg acaggctaat ctgacttctt tatcaggttc tgcgggttgc ctcgctgcta
    17641 tttcaaatgc ctttggcaca accgaatcaa atttaatgat atcggctatg tcataaacaa
    17701 atgaaagcgg tttgccacta tggataaacc caatagcggg cgcatatccc gcggctaata
    17761 ctgccgcttc agatataccg tacagacatg atgtagcagc actgatgcag cgattcacaa
    17821 catcgccttt ttcccagtct ttaggatcgt atttgcgacc attccatttc acaccatatt
    17881 gtttcgccag taatgcatac gtctggcgaa cgcgagatcc ctcaattcca cgtaactgct
    17941 ccactgaacg gcgagctggc ggtggttcac gaaaacgtaa ttcatacatt ttgcgcacca
    18001 ccttcaaccg tagatcttcc gttaaagcca gctttgccta gtagagtaat ttatctgccc
    18061 gcgcccctcc gggttgtccg gaggagtaaa cgcgaacgcc cgcttcaccg acccagacca
    18121 gcagtgttcc caccgtggcg gccagatgga ctgccgcgtg ggaaactctt gttcccggtt
    18181 cgagcataat gcaggcgacc gatcccactg gaatatgcgt gcggatcccg gttttgtcga
    18241 tcagcacgaa agcgccgtcc agtacgtcga tttgaccgta ctggaggaag atcatggaag
    18301 tgcgatcttt taatgggatc gggctcagcg gtacaaacgt cacgcctctg ctccgggttt
    18361 gatcagcatc agaccacaac cgaacgcccg gcttttgcca tacccctgac tgagacgttg
    18421 cagaaataac ccaggagcgg tgaccgtaag catccccgta taatccacgc tactgaactg
    18481 gatcagttgc cgggagtttt cccgccgcag ttgctgttgt cggtaggcat caaccgaagt
    18541 atccagcagt gtaaaaccgc ttctctcccc ctgcgctgcc agccagtcca gcgcagcctg
    18601 ttgttgatgc aaccagacat cacttccttc cacctgcccc ctcacctgcc gtttcgcctc
    18661 catcagcaag tcgtggcgtt tgcccgcttt acaaattgtt ggattcgccc gcaggttgaa
    18721 acaaagggat tgtccggtac gcaactctgg cgcaaatggt cggcattcga tggtgaaaat
    18781 atcactttcc gccgggcgtt cctgcgacag gacaaaaaag c
//
LOCUS       DAJVXP010000078        18515 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVXP010000078 DAJVXP010000000
VERSION     DAJVXP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.078"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="OQS10_002574"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="OQS10_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS2293578.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="OQS10_002575"
     CDS             737..1042
                     /locus_tag="OQS10_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293579.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="OQS10_002576"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="OQS10_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS2293580.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="OQS10_002577"
     CDS             1790..2536
                     /locus_tag="OQS10_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS2293581.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="OQS10_002578"
     CDS             2536..4632
                     /locus_tag="OQS10_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS2293582.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="OQS10_002579"
     CDS             4678..5817
                     /locus_tag="OQS10_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS2293583.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="OQS10_002580"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="OQS10_002580"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS2293584.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="OQS10_002581"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="OQS10_002581"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS2293585.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /gene="appA"
                     /locus_tag="OQS10_002582"
     CDS             complement(8566..9864)
                     /gene="appA"
                     /locus_tag="OQS10_002582"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS2293586.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="OQS10_002583"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="OQS10_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS2293587.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="OQS10_002584"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="OQS10_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS2293588.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="OQS10_002585"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="OQS10_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS2293589.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="OQS10_002586"
     CDS             complement(12875..13732)
                     /locus_tag="OQS10_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS2293590.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="OQS10_002587"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="OQS10_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS2293591.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /locus_tag="OQS10_002588"
     CDS             complement(14124..14711)
                     /locus_tag="OQS10_002588"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS2293592.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="OQS10_002589"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="OQS10_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="OQS10_002590"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="OQS10_002590"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS2293593.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="OQS10_002591"
     CDS             complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="OQS10_002591"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS2293594.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4913 c         4783 g         4275 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 tatcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg tgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc
    17701 aagcgatcaa aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg
    17761 cagccaggta ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc
    17821 gtcggattgg gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata
    17881 atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg
    17941 atacagaaca tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg
    18001 ccattgtatt gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc
    18061 atcaaagtat cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg
    18121 tgagcggagc ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc
    18181 accggaatgc ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct
    18241 aatcccagcg acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc
    18301 tgacgccgca tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct
    18361 tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc
    18421 aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg
    18481 tcgccagaaa gtattgttaa taaagcgtag tgaaa
//
LOCUS       DAJVXP010000079        17900 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVXP010000079 DAJVXP010000000
VERSION     DAJVXP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.079"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1711
                     /locus_tag="OQS10_002592"
     CDS             <1..1711
                     /locus_tag="OQS10_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS2293595.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1712..3361
                     /locus_tag="OQS10_002593"
     CDS             1712..3361
                     /locus_tag="OQS10_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS2293596.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3366..4142
                     /locus_tag="OQS10_002594"
     CDS             3366..4142
                     /locus_tag="OQS10_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS2293597.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4216..5400)
                     /gene="atoB"
                     /locus_tag="OQS10_002595"
     CDS             complement(4216..5400)
                     /gene="atoB"
                     /locus_tag="OQS10_002595"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCS2293598.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5431..6753)
                     /locus_tag="OQS10_002596"
     CDS             complement(5431..6753)
                     /locus_tag="OQS10_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCS2293599.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6750..7400)
                     /gene="atoA"
                     /locus_tag="OQS10_002597"
     CDS             complement(6750..7400)
                     /gene="atoA"
                     /locus_tag="OQS10_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCS2293600.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7400..8062)
                     /gene="atoD"
                     /locus_tag="OQS10_002598"
     CDS             complement(7400..8062)
                     /gene="atoD"
                     /locus_tag="OQS10_002598"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCS2293601.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGRAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8258..9643)
                     /gene="atoC"
                     /locus_tag="OQS10_002599"
     CDS             complement(8258..9643)
                     /gene="atoC"
                     /locus_tag="OQS10_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCS2293602.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9640..11466)
                     /gene="atoS"
                     /locus_tag="OQS10_002600"
     CDS             complement(9640..11466)
                     /gene="atoS"
                     /locus_tag="OQS10_002600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCS2293603.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11633..14482
                     /gene="rcsC"
                     /locus_tag="OQS10_002601"
     CDS             11633..14482
                     /gene="rcsC"
                     /locus_tag="OQS10_002601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS2293604.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14682..15332)
                     /gene="rcsB"
                     /locus_tag="OQS10_002602"
     CDS             complement(14682..15332)
                     /gene="rcsB"
                     /locus_tag="OQS10_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS2293605.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15349..>17900)
                     /gene="rcsD"
                     /locus_tag="OQS10_002603"
     CDS             complement(15349..>17900)
                     /gene="rcsD"
                     /locus_tag="OQS10_002603"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS2293606.1"
                     /translation="VNAWLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPS
                     SGEGLQETRLKQDVYYLEKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPW
                     SMYYLNGQDNSLVLISTLPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSN
                     MRRLAWQNGHYFTLRTTFNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGD
                     NDNEKEGTDSVSIHFNSTKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNI
                     GLLALALFGYTTFRHFSSRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQES
                     NRTVISNKIADHLLPHLNLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIF
                     IIRDQDREVLVNKKLKQAQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPE
                     SKQLANQADVLVRLVDEIQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGL
                     QLLINNHLKAHDMRRGDRDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFR
                     ILDTGEGVSIHEMDNLHFPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDG
                     LGTRYSVHIKMLAAGPEVEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITP
                     DERLISQDYDIFLTDNPSNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQ
                     LIEVQLAQEEVTESPLGGDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQ
                     TAHRLKGVFAMLNLVPGKQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4323 a         4436 c         4727 g         4414 t
ORIGIN      
        1 tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc tggcgcagaa
       61 cattcagcaa tctggcttgt taagcgcaga actgcaacaa aatgggcaag tgcaggacag
      121 cattagcaca aaactgtctt ttgtggataa cagctggccc gttgaacagc agaaaaatgt
      181 catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggctagca atatccggct
      241 acaaagtagc gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt tagtcgatgg
      301 accatggggt ggcgtgatca acaccggtag ccgtctgatc ccgctcagtc tcgcctggcg
      361 ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga ttcaggataa
      421 ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt ggggtgaaga
      481 tggcaatggt gacgccttcc ttacggcatg ggcatggtac gccgactggc aggccagcca
      541 ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca gttatgccga
      601 gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg cacaggagat
      661 gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc ggcgcggaac
      721 taaagatgaa gatttctctg aggaagacat ccgcgatatc aatgacagcc tgatcctcga
      781 tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga cgttgctgaa
      841 aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt gggataaagc
      901 ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg gtggtgacgc
      961 tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta ttgagcgcgc
     1021 gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg ttttgcctgc
     1081 gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaagact ggcgttgggt
     1141 tggtcagggt gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc aaaatgtgca
     1201 ggtccgctgg cgtgaaccgg caaaaacggc tcaacaaagt aacattccgg tgaccgttga
     1261 acgccagttg tatcggctta tccccggtga agaagagatg agctttactc tgcagccggt
     1321 gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaattacgc ttaccagcga
     1381 gcaggatgca gttctgcgct atggtcaggt ggaagtaccg ctcccgccgg gagccgacgt
     1441 tgagcgcaca acatggggca tttcagtcaa taaacccaac gccgcgaaac agcaggggca
     1501 attgctggaa aaagcgcgta atgaaatggg cgaactggcc tatatggtgc cggtgaaaga
     1561 actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag ggcaattcgt
     1621 tctgcctcct gctcgttatg tgcgttccta tgcacctgcg cagcaaagtg ttgcggcagg
     1681 gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg tctggctatt
     1741 ggcgttggtt actctgccaa cgcttgcgga ggagacgccg ttacaactgg tgctgcgcgg
     1801 tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccaaag tctcagcatt
     1861 acctgactcg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt acgcctggct
     1921 ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc cggaagaggt
     1981 ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac gttcctgtgg
     2041 gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc aatactggca
     2101 acagcgtcag gcacctgcgt ggttagcgtc tcttacaacg ctgaagccag aaacctcggt
     2161 aacggttaaa tcgctgctcg attcattagc gacgttacct gcgcaaaaca aagctcagga
     2221 agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca tgctcggtag
     2281 tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa tccgccaggg
     2341 tggttttgct ggctggttaa ccgatggtac accgctgtgg gttactggca gtggcaccag
     2401 caaaacggta ctaacccgtt atgcgacggt gcttaatcgg gttttgccag tgccgacgca
     2461 ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc tgaagaaaat
     2521 cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc gctatcgtgt
     2581 gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga ccttactgag
     2641 cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg ttgccagagt
     2701 tctggatcgc gaggcgaaaa gtacgccacc tgaggcagcg aaagccatga ccgtggcgat
     2761 acgtactttt ttacagcaaa acgccaatcg ggaaggtgat tgcctgacca tccccgacag
     2821 cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa cgatgaccgc
     2881 ttggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca gtcgcgctac
     2941 cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcaggggg agcgttacga
     3001 ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag ctccacgctc
     3061 aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc cgcagtggcg
     3121 gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg tctgtcgttt
     3181 agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc gtaacttctt
     3241 tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct ttgatggtta
     3301 tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat taatggacta
     3361 atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct gtcgctcaca
     3421 gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat ccggcagagg
     3481 gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc aacatggcag
     3541 atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct gcggcgggta
     3601 aagttcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga attgaatccg
     3661 atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg caggtcatca
     3721 gcccggatgg gcaaagccgg caaaaaatgc agttttactc aacgtcgggt ataggaacga
     3781 ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat ctcgatctcc
     3841 atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg aaaaacagtg
     3901 gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc atgccagcac
     3961 cagttcacgg gcgctatcag gtgtatatca actactatgg tgggcgcagc gaaacggaat
     4021 tgacgaccgc ccaactgacg ctgatcaccg atgaaggatc ggtcaatgag aaacaggaaa
     4081 catttattgt accgatgcgt aatgctggcg agctgacgct ggtgaaaagt tttgactggt
     4141 gattaatggg gttaccttcg ccgtcgatgt tcaagaaaac gcccgataac tttcgctatc
     4201 gggtgttttt attgattaat tcaaccgttc aatcaccatc gcaattccct ggccgccacc
     4261 aatgcacagt gttgccagcc ccagcgtttt atcgcgtgcc tgcatggcat gtaatagtgt
     4321 gaccagaata cgagcaccac tggcaccgat aggatgcccg agcgcgatgg ccccgccgtt
     4381 gacattcact ttctcagaat caaagcccag ggttttccca acggcgagga actgtgcagc
     4441 aaatgcttca ttagcttcga tgagatcaat gtctgccagt tgcagccccg ccagttgtaa
     4501 cgctttttgc gtggcaggta ctggccccat acccatcaat gcgggaggca cgccaccgct
     4561 ggcataactt ttaatgcgag ccaggggggt aaggcctgct gccagcgccg cagattcttc
     4621 cataatcacc agagcggcag caccgtcgtt aataccagac gcgttcccag cggtgactgt
     4681 tcctgcttta tcgaaggccg ggcgcaatgc acctaacgct tcagccgttg aattcgcttt
     4741 cgggaattcg tcttgactga agacgaaggt tttcttccga gtgacaacat ttaccgggac
     4801 gatttcggct gtaaaagcac cggactcaat tgcggctgcc gctttacgct gtgaatgtag
     4861 cgccagttca tcctgcattt cacgggtaat tccgtactct ttagccacgt tttcggcggt
     4921 aatccccata tgataaccat gggtggcgca catcaggcca tcgcgcagga ttacgtcata
     4981 aacctgtccg tctccaagac gataaccaga gcgtgctttt gcatcgagta agtagggcgc
     5041 taaactcata ttttccatac cccccgccac aatgctcttc gcctgacctg cctgaattgc
     5101 ctgggcggca agcgccacac ttttaagacc cgaaccacac actttattga ccgtgaatcc
     5161 gcacaccgtt tctgccagcc cgctttttaa cagtgcctga cgtgccggat tttgccccaa
     5221 cccggcttgc aacacgttac ccataatcac ttcatcaatg tgtagtgaat cgatttttgc
     5281 acgttcaatg gcggctttaa ttactgtcgc ccccaggtcg atggcgctgg tggaagcgag
     5341 tgaaccgtta aaactaccga tagcagtacg taccgcactg acgatgacac aatttttcat
     5401 tttatattcc ttctgtctgt agggttgccg tcagaacagc gttaaaccaa tgacgaaaat
     5461 gacgccggag aagagcaggg cagtgatgca atagcccatg atgtcgcgga caccgagtcc
     5521 ggcgattgcc agtgctggca gcgcccagaa tggttgttcc atgttcatcc attgctcgcc
     5581 gtaggcgatc gccattaccg atttaccgag atcagcgccc agcgcctggg ctgcgggtat
     5641 cacgaaaggt ccctgaataa cccagtgacc gccgccagac ggaacggcga agttaatcag
     5701 tgcagaacta aaaaaggtca ttaccgggaa ggtgtctttg ttcgcaacat tgatgaagaa
     5761 ttcggtaatg agtccaccca gaccagaatg ctccatcatc agttggatcc cagcgtagaa
     5821 ggggaattgc accagaatac cggcagtact gcgtgctgcc gcgctgatag cacgcatata
     5881 agccattggc gttttatgta gcaacagacc cgcaatcata aacatcaggt tgacggtatt
     5941 gatggtgatg ttgaagccat gttcgctgaa gtacatcgcc aggtaagcga taccgagtgc
     6001 gccgatgatc aacgtcagaa tgcggctttc ttccagtcgc tcggatggtg gggcatcttt
     6061 cggtagctgc ttttgaaaat cagcctcttc catgagtagt tccggatcga tactcaccac
     6121 gtcagacggt tttggcatca tcatgcgggt gataaatggc atcaccacaa tcaacgtcac
     6181 agtgatgaaa atgttaaaac cactgaacag agtatcgccc accgggatca gcccggcgat
     6241 atgctcaacc gggttgcccg gtgttgcagc caacagaggc attgatccag agaagccgcc
     6301 accccaggtg agaaaaccaa tgtaggcgca ggcaatgagc aacggataat cagaaccggg
     6361 gactcgccgg gcgacttcac gggcaaacat tgcgccgaca accaaaccaa atccccagtt
     6421 gatgacacaa gcgactgaac cgaagaaagt gaccagcatg acgccctgta cgggcgtctt
     6481 tgcggcggag gcggcagtac gcagcaaact tttcaccgga gcagagctgg caagggcatg
     6541 accggtaacg atgataagcg ccatctgcat accaaacgcc agcaagttcc agaaaccgtc
     6601 accccacatt ttcaccatgc tgatcggcgt ttgtggtgtt aaccaaagcg cgatcacgaa
     6661 tgttagcaat gtcagcaaca tggcaaagat cagtggatcg ggaagccatc ggctgacaaa
     6721 acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt attcagatcg
     6781 gcggcgactt caaaccgagc ttctgttttg gcacgcacgg tggctaaatc acacccgtcg
     6841 gcaatttcgg tgagccacat tttgccgtca ataaaacgaa agacagccag ttcagtaacc
     6901 agcatatgca ccgcatgttg cgcagtgagt ggcatggtgc agctgcgcaa aatttttgct
     6961 gaaccatctt tggcgcaatg ttccatggcg atgatcactt tgcgcgaccc ggtcaccaga
     7021 tccatcgcgc cacccatacc gggcaccatt ttcccaggca ctacccagtt cgcgaggttt
     7081 gcttcttcgt ctacttgcaa accgccgagc acgcaggcat caatatgacc gccacggatt
     7141 agcgcaaatg acatggcgct atcaaacatg gctgcaccgg gtaaaacacc gcacggttgc
     7201 ccgccagcgt tcaccagatc tggatgcgct gtcgtgaccg ggcctaaacc gaggaagccg
     7261 ttttccgatt gcagagtgat atgaataccc tccggtaaat aattggcgac cattgtgggt
     7321 aaaccgatcc ctaagttaac gatgtcacca tcacgaagct cttgcgccac acggcgcgca
     7381 atacgttgtt tcgcatccat tatttgctct cctgtgaaac gatgatgtgg tcgataacgg
     7441 caccaggggt gacaatatgg tcaggttgca gctcgccggt ttcgaccagt tcatctggct
     7501 ctaccagcgt gatatcagcc gcaagggcta tcagggggtt aaagttgcgg gcgctaagtt
     7561 gataggtcag gttgccaagt gtgtcgcaac gatgagcgcg aattagcgcc aggtcggcgc
     7621 gcagtgggcg ttcgagcagc caggttttac cgtcgagtgt cagtgtctgt ttgccttcct
     7681 ctacgacggt gccgacaccc gttggggtga gaaaaccacc aagtccagct ctaccacagc
     7741 gaatttgctc gattagcgta ccttgcggca ccagaacgac gtccatctca ccagatatca
     7801 tgcgccgacc tgtttccggg ttggtgccga tatgtgaagc aatcactttg cggactcgac
     7861 cattgacgat gagcggaccg atgccggtat caacaaacgc ggtatcattg gctatcaatg
     7921 tcaggtcgcg aacaccagat tccagtaatg cttcaaccag gcgggatgga gtgccaatcc
     7981 ccataaatcc gcccaccatg atggtcatgc cgtcacgaaa gaagccggtg gcgtcttgta
     8041 atgtcatcaa ttttgttttc attttttatc cctcttgcat acaccgttgt gggtacagtc
     8101 aggcaatagc aaggggagtg ccagaatgag attattggat ttagttattt aactaattga
     8161 atatgaaggg ataaattgga tggggattta taaggagaat gagcggctat gcagaaaatt
     8221 gcgcactgtg caaatttctg catagcaagt tttggtgtta tacatccgcc ggatcgatac
     8281 cgtattcctg gagtttatac atcaatgcac ggcgactgat gcccagcatt aaagcagtgc
     8341 gggttcggtt tccttcttgt tgttccagca cttccataat gatgcgtttt tcgacgcgtt
     8401 taatttcctc ttttaaatta cgctcaccga cagaggctgt ttttacctcg ccagcattac
     8461 agactggctg acgaatctgt ggcggaagat cctcagaaaa aatgatcggg cctgaattca
     8521 tcacgacggc gcgttcaata acgttagaaa gctctcgaat atttcccggc catgaccagg
     8581 cggtaagcag tgacattgcc atcggatcga tgtcgataat atcgcgctga ttctcactac
     8641 tgaatttttg caaaaagtga ttagctaaca gggaaatatc ttcccggcga tcgcgcagag
     8701 gcggcagtat taaatgaata acgttaaggc gataaaagag atcttcacgg aaggtgcctt
     8761 cttttaccat tgcctgcaag tcacggttgg tggcagcaat gatgcggata tcaactttta
     8821 tggtctgatg gccgccaatc cgttcaaatt cccgttcctg tagaatgcgt agtaatttgg
     8881 cttgtagtac cagcggcatt tcgccaattt cgtcgaggag cagagtacct tcgttggctc
     8941 gttcaaataa tccctgacgc aaggtttgtg caccagtaaa tgcacctttt tcatgaccaa
     9001 acagttcact ttcgagcaac gattccggca gcgccgcgca gttgacttta atgaacggcc
     9061 cctttgcccg ccgcgaattg tagtgaatcg ctctggcaat caactctttc ccggtgccgc
     9121 tttcaccgct aatcaagacg ctggcctgag aaagggcaat tttggcggtg tctttgcaga
     9181 tgtccatcat cgccgggctg ttggtgagaa tgtgccccca ttgccagctg gcgctcagtg
     9241 cctggtgcag atgacggatc tcttttttca ttgactggag ttgtaaagcg cgctgaacga
     9301 ttaaattcaa ctcatcgaga tcaaacggtt taataacata gtcgaaggct ccgcagcgta
     9361 gcgcttcgac ggcggtttcc acttccgcat aggccgtcat cagaataacg ggtgtccggg
     9421 tctcatggct gcgcatctcc tttagtgcct tgatgccgtc catctctggc atgcggatat
     9481 ccatcaacac cacatcaggg tgaatatcgg caaacaggtg taatgctgtg cgtccgttgt
     9541 tcgcacaatg tgtttcgaat ccttgtagtg caaaagcggt gctcagcata cggcgaacat
     9601 tatcttcatc atccacaata aggatgcgat taatagcagt catacagtct gatttccctg
     9661 cgggttgatc ggtaaaataa gcgtgaaggt tgcgccgtag cccggcaaac tggcgacgcg
     9721 aatatcaccc tgatgggcat taatgatgcg ttgacttaac gccagaccaa gcccggttcc
     9781 tgaggctttg gtggtgaaaa agggatcgaa gatctttttt tgcagcgaga gatcaatgcc
     9841 acagccgttg tcctctatcg aaatggcctg ttgtgagtcg ctgtattgcc aggtttgaat
     9901 gcgaattttc cctcgtgcgc tgatagcctg gacggcattg atcaggatat tcagtagtac
     9961 ctgtttgagc agttcacgat cggcgttaat cgggcttaat tcattatcca gttcgcttat
    10021 gaagtcgacc cgcgcttgta cgccggcggt ttgtaccagt accagagttt cttcaaccaa
    10081 tgcattgagg ctgacttgtt gccattgact gtggcgtgga cgtgaaaatt cgagcaattg
    10141 ctgaataact ttgttaattg aatcgatttc tttgagtact acggacagat attcctgatg
    10201 tattgggtca ctggtttgtt ggcgcaagat ctgtacataa ccacgaatag ccgttaacgg
    10261 attacgtact tcatgcgcga cgccagccat cagctcaccc agtgtggcga ggcgttctgc
    10321 ttgcgccatg cggcgctggg tttctttgcg ggcagttaaa tcagagaaaa tcaccaaagc
    10381 acctatcatt tcaccgtgcg tgttatgaat acgactggta gtgacactga gttcaatggt
    10441 gcggtcacga cctggaaaac tgatctccag cgccacatgt tcggtgccat gttccagcgt
    10501 atccagtact ggactgtaga actgagtatt gtcgaacaac atggagtaag gctgccctac
    10561 cagttcatgg cgttgatagc cagtgataac ttctgctgct gggttcatgg tggttacatc
    10621 accctggcgg tcaatggcaa tgacgccatc ggcagcgttt tcaataatca gatcgttaag
    10681 tgtccgcgtt tcacgcagtg cctgggcgag gttattaaca ctctgactga tttgccccat
    10741 ttcaccgggc aattgtggta atcgagtggg aatattttgt gccagagtcg agaggccatc
    10801 ggtgatgata tcaatattgg cgctcaggcg acgggagaaa aggacaatca atagcaggct
    10861 tatcagcaaa tcggcggtga gcacaatgat aatcctcaca tccattttcc aggcctggcg
    10921 gcgaatatct tcggttaatt cattggccca gatatagccg aggatttcac cattacgctc
    10981 aatggggagc attgaattca aaatatcgcc gcgcacctgc ctgcctgaat aaaccaaagg
    11041 ggtatttgta cgcatgactt cgcgaccagg gtgatctgcg gcaatggtaa cgccgacatt
    11101 attctgatat agcgctgaag gcgcgtaggt gattatcgca tccagcattt tgttgtaata
    11161 accagcacct atgccaggga aggcgtgagt gatattttcg gtaatggggg caagttctgc
    11221 atttaatgcg cggatacgct cctcacgtgg taagtcgatg tagagatcat agcgatcgcc
    11281 tagtgcctga ttaagcaggt tgaccacggc agaaagtttt ttctcttttt cagataagac
    11341 tgctgaacgt ccttccgttt ctacgatata accaatagta agcgttggga caatgaccat
    11401 caggattgcc atcaggatca tttgattgcg taagcggcgt ggataaatcc acttcatata
    11461 atgcatgctg aaatccacta gtcttgtccg gtatatgacg attatcagag gttaaggtga
    11521 tgatttctcg gcggtgtatc atattccaga gaagagagaa cattgcggta acacgctttt
    11581 accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata
    11641 ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt
    11701 agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca
    11761 gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat
    11821 tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc gcttatcggc
    11881 agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga
    11941 accgctgttt gccgactccg attgttccgc aatgagtaac acctggcgag gttctctgga
    12001 gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctaaa
    12061 ccgggtattt ttaatcggca gcgataacct ctgcatggcc aatttcggtc tgcgtgatat
    12121 gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa cgcatcaata aatatcgaaa
    12181 tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg
    12241 cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc aggcgctttt
    12301 gggtgtcgag cagaccatcc ggatggagaa ctttttctta ccgggtacgt tgccgatggg
    12361 ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagtaa
    12421 aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt
    12481 ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tgtattcggt
    12541 gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa
    12601 tgtgcttgcc ggagctgcat tgtttactct cgcacggatg tacgagcgac gtattttcat
    12661 tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc gcaagattgt
    12721 cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag
    12781 taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacgactgac
    12841 gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca acaataccaa
    12901 tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct
    12961 ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc
    13021 ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac
    13081 gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg
    13141 cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag
    13201 cgatattctc gatttctcga agattgaatc ggaacagttg aagatcgaac cgcgtgagtt
    13261 ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa
    13321 gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc
    13381 gatgcgttta cagcaggtca tctccaacct gttgagtaac gccataaaat tcaccgatac
    13441 cggctgtata gttttgcatg tccgcgctga tggcgattat ctctctatcc gtgttcgcga
    13501 taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt
    13561 cggaacgggc gtacagcgaa acttccaggg gaccggtctg ggtctggcga tttgtgaaaa
    13621 actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa tgggcagcca
    13681 gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg
    13741 gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga
    13801 aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc
    13861 cgaagatgtg ttaattactg acgaggtagt gagtaaaaaa tggcagggta gagcggtagt
    13921 gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca
    13981 cagtgtggct gccccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat
    14041 ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataatga
    14101 cgatatgatg attctggtcg tggatgatca tccgattaac cgacgtctgc tggcagatca
    14161 gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggtgtcgatg cgcttaatgt
    14221 acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa atatggatgg
    14281 ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac
    14341 tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagctgcct
    14401 gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt
    14461 caggaaatcg cgggaatcgt aggacggata aggtgtttat gccgcatctg gcattcagtg
    14521 caaatgccag atgcgacgct gacgcgtctt atctggccta ctttgatgta atctgttgaa
    14581 ataatgggaa tcgtaggacg cataaggcgt ttacgccgca ttcggcaatc ggtgcaaatg
    14641 ccagataaga cactaacgcg tcttatctgg cctacaggtg attagtcttt atctgccgga
    14701 cttaacgtca ctgaagagag ataattcagc agggcgatat cgttctcgac acccagcttc
    14761 atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag ctttttagcg
    14821 atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa cttcactctc ttttggcgag
    14881 agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga aacgctttcc
    14941 ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt tcggcagatc ggtcggtgca
    15001 ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag aatcgccggg
    15061 ttgttgttca tagtcagaac aatgatcgac aggcttggga aatggcgctt gatgtacttg
    15121 attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt aatcaacaca
    15181 tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa ttcgccgaca
    15241 acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac tatcggatgg
    15301 tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct acagcaagct
    15361 cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat ccttctcacg
    15421 aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta gcatggcaaa
    15481 tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg ttgctgcttc
    15541 agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat aatagccgct
    15601 ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct cttcctgcgc
    15661 cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca tattgaagtt
    15721 gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc ttaaaagcaa
    15781 gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat cttgactaat
    15841 taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc gagtgacaat
    15901 attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca gtaaacgctc
    15961 ttcttcctct tcaacttccg ggccagcggc gagcattttg atatgcacag agtagcgtgt
    16021 accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgtg ccagttgatc
    16081 gctcagccag aatgccagcg ggtccgcctt tccataacga tcgttttggg tctggttgat
    16141 aaacgggaag tgcaaattat ccatttcatg aatacttacg ccttctccgg tgtccagaat
    16201 gcggaatgtc aggcggtctt cagaggactc atcctgatca acctcaaggg tgatttttcc
    16261 caattgcgtt gaggtcacgg catattgcat cagtagcagc aaaatacgtc gtaaggcatc
    16321 gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca gttgcagacc
    16381 tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat cctgcacgga
    16441 gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta actgtatttc
    16501 atcgaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt ccggggcgtt
    16561 gagtttagcc gcgctctccg ccagggactg tgcaggttct ttcagcgcat cgccaatgtt
    16621 tttcataaag gtcatccggc cctgctggtt tttctcatac agacgctgcg cctgcttgag
    16681 tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg
    16741 cgcgacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg tcgcctgaat
    16801 aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat gcggcagcaa
    16861 atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag
    16921 gccgagcggc agcagtgaga ctatcttttc attgattgcc cgtaaaatgc gcaattcgtt
    16981 attgaccgcc gtgttgggta gactttctgt actgcgactg gagaaatggc ggaatgtggt
    17041 atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt
    17101 ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt
    17161 agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc
    17221 cgttccttct ttctcattat cattgtctcc cgttgccgtc gtgtctggct caaggcggaa
    17281 actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacgac
    17341 cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat gaccgttctg
    17401 ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt tggcctgttg
    17461 caacatttct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt taaatccgga
    17521 ggtgagatct ttgaggggca gggttgagat cagtaccaga ctgttatcct gaccattcag
    17581 gtaatacatc gaccacggta cattttctgc accccacaat gtatccagat aagtggacat
    17641 ccgctgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt
    17701 gcggcgtggt ttctccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc
    17761 gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg
    17821 ccaggtatcg acgcgctttt ggattgcgtg ggtaatgtcg acaatctggt aacttttatc
    17881 tttcagccag gcgttaacgg
//
LOCUS       DAJVXP010000080        17840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVXP010000080 DAJVXP010000000
VERSION     DAJVXP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.080"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..716
                     /locus_tag="OQS10_002604"
     CDS             <1..716
                     /locus_tag="OQS10_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2293607.1"
                     /translation="RHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRISQNPV
                     TPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSD
                     IYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGIRKNSP
                     DGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHT
                     SEKTTKMYLDEREKTYLLL"
     gene            complement(1109..1450)
                     /locus_tag="OQS10_002605"
     CDS             complement(1109..1450)
                     /locus_tag="OQS10_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS2293608.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1463..2335)
                     /gene="copD"
                     /locus_tag="OQS10_002606"
     CDS             complement(1463..2335)
                     /gene="copD"
                     /locus_tag="OQS10_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCS2293609.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2339..2713)
                     /gene="yobA"
                     /locus_tag="OQS10_002607"
     CDS             complement(2339..2713)
                     /gene="yobA"
                     /locus_tag="OQS10_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS2293610.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2852..3082
                     /gene="holE"
                     /locus_tag="OQS10_002608"
     CDS             2852..3082
                     /gene="holE"
                     /locus_tag="OQS10_002608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS2293611.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3184..3840
                     /locus_tag="OQS10_002609"
     CDS             3184..3840
                     /locus_tag="OQS10_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS2293612.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            3864..4526
                     /gene="exoX"
                     /locus_tag="OQS10_002610"
     CDS             3864..4526
                     /gene="exoX"
                     /locus_tag="OQS10_002610"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS2293613.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4523..6583)
                     /gene="ptrB"
                     /locus_tag="OQS10_002611"
     CDS             complement(4523..6583)
                     /gene="ptrB"
                     /locus_tag="OQS10_002611"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS2293614.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6792..7451)
                     /locus_tag="OQS10_002612"
     CDS             complement(6792..7451)
                     /locus_tag="OQS10_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS2293615.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(7778..8134)
                     /locus_tag="OQS10_002613"
     CDS             complement(7778..8134)
                     /locus_tag="OQS10_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS2293616.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8201..8491)
                     /gene="yebG"
                     /locus_tag="OQS10_002614"
     CDS             complement(8201..8491)
                     /gene="yebG"
                     /locus_tag="OQS10_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCS2293617.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8625..9803
                     /gene="purT"
                     /locus_tag="OQS10_002615"
     CDS             8625..9803
                     /gene="purT"
                     /locus_tag="OQS10_002615"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS2293618.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(9859..10500)
                     /gene="kdgA"
                     /locus_tag="OQS10_002616"
                     /gene_synonym="eda"
     CDS             complement(9859..10500)
                     /gene="kdgA"
                     /locus_tag="OQS10_002616"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS2293619.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10537..12348)
                     /gene="edd"
                     /locus_tag="OQS10_002617"
     CDS             complement(10537..12348)
                     /gene="edd"
                     /locus_tag="OQS10_002617"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS2293620.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12583..14058)
                     /gene="zwf"
                     /locus_tag="OQS10_002618"
     CDS             complement(12583..14058)
                     /gene="zwf"
                     /locus_tag="OQS10_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS2293621.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14396..15265
                     /gene="hexR"
                     /locus_tag="OQS10_002619"
                     /gene_synonym="yebK"
     CDS             14396..15265
                     /gene="hexR"
                     /locus_tag="OQS10_002619"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS2293622.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15393..16835
                     /gene="pyk"
                     /locus_tag="OQS10_002620"
     CDS             15393..16835
                     /gene="pyk"
                     /locus_tag="OQS10_002620"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS2293623.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16895..17737)
                     /locus_tag="OQS10_002621"
     CDS             complement(16895..17737)
                     /locus_tag="OQS10_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCS2293624.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4514 a         4511 c         4323 g         4492 t
ORIGIN      
        1 ctcgccatat tgccgagttt cttgatttgt ggattgaagg agggaaaaac acaatggcag
       61 gatcaatgcg ttctgtgttg tctgatatgt ttcgcgaggc cattgttgaa ggacgtatat
      121 ctcaaaatcc agtcacgcca acaagagcac cgaaaatagt agttacaaga gaacgactga
      181 aactaaagat atacaactgc atcagggagg cagcagatca acttccggca tggttcccat
      241 tagctatgga tttagccctt gtaacaggac aacgtcgcga agacataacg aatatgcggt
      301 tcagtgatat ttatgatgat cgtctccacg tcaggcaaat taagacagga atgatgattg
      361 ctatccccct gtcactcagc cttcctgtcg ctggtctacg gcttggtaca gtagttgaac
      421 gatgccgcct ggtaagccgg ggagattatc taatcagtgc cgggattaga aaaaacagcc
      481 ctgacggcag cattcacccg gatggcctga caaaaaaatt tgtcgcagcc agaaaattca
      541 caggtatcca gttcagtgaa aacccaccaa cttttcacga gatcagaagc ctggctggac
      601 gattgtacaa agaaacatgt ggagaagaat ttgctcagcg tctacttggc cacacatcgg
      661 agaagacaac aaaaatgtat cttgatgaga gagaaaaaac gtacttactg ctctgatttt
      721 aacgtaaatg gattgttaaa tgtattttgg ttgtgatata accaaaaaga ccggaataca
      781 gaaattcgag taaatttcgg ggaatttcgg gggagacatt tgcaactaat tgatttcaaa
      841 tgcaattaaa aaaagaccga atacgattcc tgtattcggt ccagggaaat ggctcttggg
      901 agagagccgt gcgctaaaag ttggcattaa tgcaggctta gttgccttgc cctttaagaa
      961 tagatgacga cgccaggttt tccagtttgc gtgcaaaatg gtcaataaaa agcgcggtgg
     1021 tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa gaactgaggc ggttttttta
     1081 ttggaaatca aaaggctatt ttaggtaatt aacagagttt ttcagctcgt tctataaacg
     1141 gtgccagact cattttttcg ccgggattgt taggatcatc aatctgaatc accgaaatgg
     1201 gttgggcatt ggtcttccca ctggcaactt ccttttgtgc gatatcgttt aaaggatact
     1261 gcacgagggt actcggatta ataacataca aagcattacc cggtcggcaa gtcagcatca
     1321 cctcttcgcg attaaacgcc catttgtctt tacccacttc aaaacgactg acggtaatca
     1381 cctgcggtgc agccagcgcc gctgcagaac tggtgagtaa cagaaacgcc agaatacttt
     1441 ttttcatcat tttcgtaatc catcaaaaag gttcgagagt cgcaagcagg ctgatgatca
     1501 ccagcactac cgcacctatc gcccattcga gtttagtcat ccacacaaac cagggtgccg
     1561 cacgatcttc ctcctgcctc atgcgcggta caagaacata acgattagcc agcgcaatga
     1621 ccaccataat catcaccagg atggctttga gcaataagag ttggccccag taagtcgtaa
     1681 gcgtgggggg aaatccggta atcaacaatg cattaagcac gccgcttgcc agtacgccga
     1741 tcaccgcaaa atgcccgcac catgaaaaac acatcagcgc ctgaatagcc tggtgtcgcc
     1801 aacggccttt gatgagttgc atacaccaga gcaccggcag taatccccca aaccacgccg
     1861 ccgcacaaat caggtgaatc gcgtgattag tctgatggat tttcgccgtt accccttcat
     1921 tcagtgtcgc atgccccatt cccgccagca gaataaattg cgcggtagtg agcataaaca
     1981 gcaaacgtgg catattccgc ggttgcataa gggcgacgat caacgttacg agggcgagaa
     2041 caatttgcca taaccagacg ccaccaaact gcgtttgcaa aacagcctgc caaatgtttg
     2101 gcgaaaatac atctgtccat cccgttccca tcaacccgcc ctgaacggcg agcattgcag
     2161 tggcactgat caaactccag acggcagcgt gctgttgtaa acgcagaaaa cgcttcgcca
     2221 gcaagcgacg aatcgtcaag ggggccagcc aggcaccgta cattgcgaag ccaaaaacca
     2281 gcatcagcga ggtgaaatgg ataaatcgca gcgcgatcca ggtaaacgcc agcatgattt
     2341 atttcacgct aaaggtgtag tgtcctttcg ttttatgccc gtccaccgaa acaacatgcc
     2401 agtcaacggt gtaggttccg ggtttcagcg aatcggccag tggaacaatc agttgctttt
     2461 ggtcctgctc atttcgcttc gccggtaatg ttttaatatt ttcgtttttt ggccccgtga
     2521 tttttgcacc actgaatcct gtttccacac cttccgagaa gtttaaagtg attgcctgcg
     2581 gtgcagctgt cacttgcgcg tttgccgcag gatactgatg cgttaaatgc gcatgtgccc
     2641 agacgaaagg tgtcaccaac gaagtggtca ggattgccag cgcgtagcga agggagcgtg
     2701 cagttgaagc catattatct attccttttt gtaataactt ttttacagag cataaccttg
     2761 tctaatgtct gagtcgagga tcatcaattc cggcttgcca tcctggctca ctcttagtaa
     2821 cttttgcccg cgaatgatga ggagattaag aatgctgaag aatctggcta aactggatca
     2881 aacagaaatg gataaagtga atgtcgattt ggcggcggcc ggggtggcat ttaaagaacg
     2941 ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa cagcctgaac atttgcgcag
     3001 ctggtttcgc gagcggctta ttgcccaccg tttggcttcg gtcaatctgt cacgtttacc
     3061 ttacgagccc aaacttaaat aaaacttata cagggttaca ctttcttaca taacgcctgc
     3121 taaattatga gtattttcta aaccgcaccc ataatttgca gtcattttga aaaggaagtc
     3181 attatgtcgt tctggaaagt tgcagcagcg caatatgagc ctcgtaaaac ctcactcacc
     3241 gaacaagtag ctcatcatct ggagtttgtg agggccgccg ccaggcaaca atgtcagctg
     3301 ttagtttttc catcgctttc tttactgggg tgtgattatt cgcgacgtgc ccttcctgcc
     3361 ccacccgatc tctcactgtt agacccgctt tgttatgccg caacgacctg gcgaatgacc
     3421 atcattgccg gccttcccgt tgaatataac gatcgcttta ttcgtggaat tgcggtgttc
     3481 gcaccgtggc gaaaaacgcc tgggatctac catcagagtc atggtgcatg tctgggccgt
     3541 cgttccagaa ccatcacggt ggtcgatgaa caaccggaag gtatggatat ggacccgacc
     3601 tgttcactgt ttacgacagg gcaatgtctg ggagaacccg acctgctggc gtcggcccgt
     3661 cgcttacaat ttttttcaca tcagtactcg attgctgtgc tgatggcaaa tgcccgtggt
     3721 aacagtgcac tatgggatga atatggtcgt ctcatcgttc gcgccgatcg tggttcatta
     3781 ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg atatcattcc attacgctag
     3841 gctttttcgg cctggagtat gccatgttgc gcattatcga tacagaaacc tgcggtttgc
     3901 agggagggat cgttgagatt gcctctgttg atgtcattga cggaaaaatc gtcaacccca
     3961 tgagccacct ggtgcgcccc gatcgtccta ttagtccaca agcgatggcg attcatcgca
     4021 tcaccgaagc catggtcgcc gataaaccgt ggattgaaga tgtgatccca cactattacg
     4081 gcagtgaatg gtatgtcgcg cacaacgcca gctttgaccg ccgcgtactg cctgagatgc
     4141 ccggtgagtg gatttgcacc atgaaactgg cccgtcgctt gtggccagga atcaagtaca
     4201 gcaatatggc gttatataaa acacggaagc tcaatgtaca gacgccgccg ggcctgcatc
     4261 atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt gcttatcgat attatgaaca
     4321 cctccggctg gacggcagaa cagatggccg atatcaccgg acgtccgtcg ttgatgacga
     4381 ccttcacctt tggcaaatac cgtggcaaag cggtttccga cgttgccgaa cgcgatccgg
     4441 gctatctgcg ctggttattt aataacctgg acagcatgag cccggagctg cgtttaacac
     4501 tgaaacatta tctggaaaat acttaatccg caggcgtagc gggtaatgtt ccctgcgcca
     4561 gcgcgaccag aaaagcatat tccatcgcta cgccttcgta cgatttaaag cggccagatt
     4621 taccgccatg gcctgagtcc atgtcggtac agagcaataa aagatggtta tcggttttca
     4681 gctcgcgcaa tttagcgacc cattttgccg gttcccaata ttgcacctga gaatcgtgta
     4741 aaccggtcgt taccagtaaa tgcggataag cctgtgcggt gacgttgtca tacgggctgt
     4801 agcttttcat gtactcgtaa tattgcggat cctgcgggtt accccactct tcaaactcac
     4861 cagtggtaag aggaattgat tcatcaagca tcgttgttac aacatcaaca aacggtacct
     4921 gggcgataac gccgtggaat aattccgggc gttgattaat tgcaacgccc atcaacatgc
     4981 cccccgcact cccgcccatc gcataacaaa gcgaaggaga gccatagccc agttttaaca
     5041 atgcatcgca ggcatcaaga taatcattaa acgtattttt cttcttcaga aattttccgt
     5101 cttcgtacca ttgttgcccc agctcaccac cgccgcgaac atggacaatg gcgtagacaa
     5161 agccacgatc taacaaactc aagcggctaa aactgaaatc ggcatcaata cttgcgccgt
     5221 aagaaccata gccatacacc agcaacgggt tgtgtccttt gcgaaaatgt ttgcgatggt
     5281 agaccaacga aaccggaact tcgacgccat cacgggcgac tatccacagg tgttcactgc
     5341 ggtaattcgc cgcatcaaaa ccaggaactt ccgtttgttt taatacacga cgctcatcgg
     5401 tatccatatc cagttcaaac aaagtgtctg gtgtggtcat ggaagaataa ccataacgca
     5461 atcgcgcggt ttcaggttct ggattgtagg caatccaggt cacataggct ggatcatcaa
     5521 aggcaatacc aatgacttcc cgggtcttgc ggttaatttg gcgcaaactg gttaacccgc
     5581 gctgacgctc ttcaaccacc agccagtcgg taaacagcgt aaacccttcc agcatgatgt
     5641 tttcgcatgg cggaattaac tcttcccact gttgctcatc acgcatacgg gtacggtata
     5701 agccaaagtt tttgccgtgg cggttggaac gcagataaaa acgatgctgg tagtgatcaa
     5761 ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa cggctcggca tcggccattt
     5821 ccgcgtccag caggcgaact tcactggtgg tggcgctggc caaatgaatg actacatagt
     5881 gcttcgaggt cgttttatgc aggctgacgt aataggtatc gtctttttct tcgtagatca
     5941 gtttatcttg cgatgctggc gtaccgatgg cgtgacgcca gacctgataa ggcagcagcg
     6001 tcaccggatg cttgcgaaca tagtagaaag tccaggagtc atttgcccag acaaagctgg
     6061 gttcaacgtt atccagcagt tccgggtacc agttaccagt ttccagatta cgaaaacgaa
     6121 tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc cataatggtg ttatcgggcg
     6181 taatcgccat tccgcccatc gaataaaact cactatgagc tgcgcgctta ttggcatcga
     6241 gcaatatttc ccactcatcc cactcttcac tgaatgccga ttgacgctgg tagatagcat
     6301 attcacagcc tggttcataa atatgccgat agcggtagcc atttttgatg tagggcgcag
     6361 aaacttctcg ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc tgcaaggctt
     6421 gttgtgaggc catcacccga tgaccgtaac tattttcttg ttgcaggtag tccaggactt
     6481 ctggctgaga acgcgtatca tcccgcagcc agtagtaatt atcgatgcgc gtatcgccat
     6541 gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag catgttattg ttctttctgg
     6601 ttgaaacatc ttataagggt ggcaaaactc accggggatg caagcgaaac aggggagtca
     6661 ttgcttagat gatgacaggt aatggcacgg atatcgaatg ttatgcagac agagaaactc
     6721 agcctgttca aatggctgtg cggttctgga tagcccgaaa tagtcaactt caggctatcc
     6781 agagagcgga attattccgc caaagtgcgt ttttgctgtt cgagatcgcg ttcaatgccg
     6841 tcacgaacat cctgggggat tttcagcgcg tcacccagtg cattcaggta actgcgttcc
     6901 ataaaatggt caatatcaat agccgcgcaa ctcaggaaat agatttccag cgcctcttct
     6961 tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat tgcctgctca
     7021 atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctggtc gattgccgca
     7081 cgttctttgg catcaatatg accatcactt ttagcggcaa agaccagcgc aaggatcaaa
     7141 cgttctgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc gtcctgatgc
     7201 gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac cgctccgccg
     7261 ccaaccagta acgcgttggt gccatatttc gtaagaagtt tacgtgctga tttatttgcg
     7321 accagcagcc cagccagtcc gcctaatgcg cctggcacta acagtttgcc caatccctga
     7381 tccgcagacg aggaggtaga agaactgctt tgcccaagca gggattgcag ttgatttaac
     7441 cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata gcggatgagg
     7501 atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgg taggccgtta
     7561 ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat cgggtgataa gggtatttta
     7621 ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc gatgcgacgc
     7681 tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg ggcggacaag gcactcgcgc
     7741 cgcatccacc aacggtcaat cgaattatca gaagccatta acgccgctga tattccgcca
     7801 ttcccgcttt gcagtccacg ctgacctggt aatgaatatc ggcactttta ccacgcacgg
     7861 ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg acccaagcca
     7921 cgggatcggc ctgaccgaca attttttgat catctgccca acgcgccacg cgattttgtt
     7981 gataatcacg ttttacgctc gcggcaattc cggcagcatc cagaccttca cactttggga
     8041 aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag gcagaaacca
     8101 acaacagccc taaaaacgcc cctctttttt tcatgttttt ctccatagca caatgattca
     8161 ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg tgtgaggcat
     8221 cttcctcttc ggattcagca ccgacgacct gtggggacgg taatttgccg gttttcagaa
     8281 tggtgctcag cacatctttt tgttctgcca gccatagcga aagggcttca cgttgctcgt
     8341 cttccatttg cactggagaa tttgtcagcc aggtgtcaag cagatccgcc gtatcaagca
     8401 ttttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct tcgccctcac
     8461 gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa ctgtgatttt
     8521 atacagtata tttcttttcg gttgagaaat caacatcagc aataaagaca cacgcaaacg
     8581 ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg ttattaggca
     8641 ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa ctgggtaaag
     8701 aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat cgctatgccg
     8761 acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt gatggtgatg
     8821 cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag atcgaagcta
     8881 ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc ccctgcgctc
     8941 gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca gaagagctgc
     9001 agctgcccac ttccacttat cgttttgccg atagcgaaag ccttttccgc gaggcggttg
     9061 ctgacattgg ctatccctgc attgtaaaac cggtgatgag ctcttccggc aaggggcaga
     9121 cgtttattcg ttctgcagag caacttgctc aggcatggaa gtacgctcag caaggcggtc
     9181 gcgccggagc aggccgcgta attgttgaag gcgtcgttaa gtttgacttc gaaattacct
     9241 tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accggtaggt catcgccagg
     9301 aagatggcga ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt gcccttgaac
     9361 gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggt ctgtttggtg
     9421 tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct cgtccacatg
     9481 ataccgggat ggtgacgtta atttctcaag atctttcaga gtttgccctg catgtacgtg
     9541 ccttcctcgg acttccggtt ggcgggatcc gtcagtatgg tcctgcagct tctgccgtta
     9601 ttctgccaca actgaccagt cagaatgtca cgtttgataa tgtgcagaat gccgtaggcg
     9661 cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt cgtctgggtg
     9721 ttgcgctggc gaccgctgag agtgttgttg acgccattga acgcgcgaag cacgccgccg
     9781 gacaggtaaa agtacagggt taaacccggc aaaaaaacgc tacaaaaatg cccgatcgta
     9841 gatcgggcgt tttgactttt acagcttagc gccttctaca gcttcacgcg ccagcttagt
     9901 aatgcggtcg taatcgcccg cttccagcgc atctgccgga accagccagg aaccaccgat
     9961 gcacagcacg cttttcagcg ccaggtagtc acggtagtta gccggagaaa taccacccgt
    10021 cgggcagaaa cggacctggg agaacggacc cgcgatcgcc tgcagggctt tcacgccgcc
    10081 gttagcttca gccgggaaga atttgaactc tttcaaaccg tagtccatac ccagcatcag
    10141 ttcggaaaca gtgctgatcc ccggaattag aggaatagtc ccttcggtag cagctttcag
    10201 cagcggctcg gtcagacccg ggctaattgc gaactgtgca cccgcttcag tgacttctgc
    10261 cagctgctgt ggattcagca ccgtaccggc acccacaatc gcttcaggca cttctttggc
    10321 gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga gtcacttcca gaacgcgcac
    10381 cccaccagca accaacgctt ttgccatcgg cactgcgtgt tccagttttt ttaccacgat
    10441 aaccggtaca accgggccgg tggtcaggat tgattctgca cttgttttcc agtttttcat
    10501 cagagttttc tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac aggttgcgcc
    10561 ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc ctgtccccac
    10621 gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg cttcgtctac
    10681 cagcagcgtc agttcgcctg tctgtccatt cacacgaatg atgtccccgt cgcgcacttt
    10741 tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg atagctgacg gcactttacc
    10801 tgaagcgccg gagagtcgtc catcggtaac taacgcaatt ttgaaacacc ggtccaataa
    10861 tacaccaagt ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt ttggcccctg
    10921 atgacggaca acaacgacac aatcgcggtc cagcaaaccc gcttcaaagg ccggcataac
    10981 gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc acctggttct caaccggcac
    11041 ggcagaggtt ttcataaccg cacggcccag gttaccgctt aacacttttg tcccaccatg
    11101 atgagagaaa ggttgttcga aggaagcgat cacattgctg tcgagtgatt tttccgcccc
    11161 ttcccgccag tcctgttcac cattattcag ccatggttca agggtataac gagacagacc
    11221 aaagcctgcc accgtattga catcttcatg cagcaggcct gctttgagca gttcacgcac
    11281 cagaaccggt acgccacctg ccgcctggaa gtggttaata tcggccggac cgttcgggta
    11341 gagacgtgcc atcagcggta caacatcaga aaggtcagag aagtcatccc agttaatctg
    11401 aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg tggttagtgg aaccaccggt
    11461 cgccagcagt gcaacgatac cgttcaccac cactttctca tcgatcatct taccgatcgg
    11521 catccattca ttaccattac cggtcatgcg tgtaacctga cgcgcagcgg cagcggtcaa
    11581 agcatcgcgc agcggagaat ccggatgaac aaaagaagag cctggcaact gcatccccat
    11641 aaattccacc accatctggt tggtgttggc agtaccgtag aaagtacatg ttcccggcgc
    11701 atggtaagac gcggcttctg actccagtaa ggccatgcgg tccactttac cttcggcata
    11761 aagctggcga atacgcactt tttctttatt tggcaaaccg cttgccatcg gtccagacgg
    11821 cacaaacacc gcaggcaaat gaccaagcga cagggctgcc atcgtcagac ccgggacaat
    11881 cttgtcgcac acaccgagga acagagcacc atcaaacatg ttatgggaca gccccaccgc
    11941 cgcagacatc gctatcactt cgcggcttag cagcgacaat tccattccat cctgcccctg
    12001 ggtgacacca tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg cattcgcttc
    12061 atgcagggct ttacgaatga tttctggata gtgttcataa ggctggtgcg cggagagcat
    12121 gtcgttatag gaggtgatga tggcgatatt gttacgcaac atgcttttca aagaggcttt
    12181 gtcttctggc tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca actgcgaacg
    12241 atgaacggtc gacgttttcg cttgttctat ccgggcgaga taagcagagc gagtctcgcg
    12301 cgaacgttca atgattcgat ttgttacgca taacaattgt ggattcataa aggctcctga
    12361 aattgagttg tcagagcagg atgattcaca acgcgtttca ttcagaggat ttatgagtga
    12421 aacgcctgta accggagctc atagggcaaa aacgtttcag tcagtgtaat aaaaaaagcc
    12481 tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt gttctacaat ctgtgcaaga
    12541 tcatgttacc ggtaaaataa ccataaagga taagcgcaga tattactcaa actcattcca
    12601 ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg ggtccccagg ttccggcctg
    12661 atacggtttc ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt ctacccattt
    12721 ccaggcttct tccacttcat cacgacgtac aaacagtgcc tgaataccac gcatggtttc
    12781 cagcagcaga cgttcatagg catccgccag atgcgtctga ttaaaggttt ctgaatagct
    12841 cagatccagc ttggtgattt gcaggttatg tttgtggtca aggccaggaa ctttattcag
    12901 tacctggata tccacgcctt catcaggttg cagacggata gtcagtttat tctgcggcag
    12961 atcctgccac gactctttaa acagattcag ttcaggtgtt ttgaaataga ccacgacttc
    13021 agaacatttg gtcggcagac gtttaccagt acgcaggtag aatggcacac cggcccagcg
    13081 ccagttatca atgtcgacgc ggatcgccac gaaggtttct gtattgctgc tcttgttcgc
    13141 gccctcttct tccagatatc ccggcacttt tttgccctgg gcgaagcccg cagtatattg
    13201 cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg cggcgcaggg acttcagtac
    13261 tttcactttt tcatcgcgga tgctgtctgc gctcaggtca gatggcggag acatcgcaat
    13321 catgcagaga atttgtagca ggtggttctg aatcatgtca cgcatctgac cggctttatc
    13381 aaaatagccc cagcgccctt cgatccccac ttcttctgcc acggtaatct caacatgatc
    13441 aatggtgcga ttgtcccagt tattcacaaa cagggagtta gcaaaacgca gcgccaacag
    13501 gttcagcacc gtttctttac caagatagtg gtcgatacgg taaacctggc actcctcgaa
    13561 gtattcgcca acctgatcat tgatttcctg cgaggtcgcc agcgacgtcc ccagcggttt
    13621 ctccatgact acgcgtgccg gtttagcatt cagttttgcc tcgccaagcc ctttgcaaat
    13681 tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaatg gtgatacgat ttttttgatc
    13741 cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg acatcgagat tacaaaaatc
    13801 cagacgtgca ctcagggtgt cccataaacc ttcatcaatg gtttctttca tgaaagtttc
    13861 gagcgcctcg cggacaactt tggtatatgc cgctttatcc cagtcagcac gccctacgcc
    13921 gataatccgg gtgtccgggt tgagctgacc ggctttttcc agttgataca gggaaggcag
    13981 caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac aggcctgggc
    14041 tgtttgcgtt accgccatgt cattctcctt aagttaacta acccggtact taagccaggg
    14101 tatacttgta attttcttac ggtgcactgt actgctttta cgagcttgcg aaaactgtaa
    14161 acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa gtgcggcaaa actgataaaa
    14221 aaatcatcgt tttccattcg gtaaaaatct gacactgatc atgttatgaa aaaaaataac
    14281 aactttttta tctgcttttg tcattaacac ggcacacagg cgtaatatct gacaaaactg
    14341 catttcgatt tctttcagtg cggaaatcgt cattacccgt gagtctcttt acatcatgaa
    14401 tatgctggaa aaaatccagt ctcagctgga acatttgagc aaatcagagc gcaaagttgc
    14461 cgaggtcatt ctggcttcgc ccgataacgc gatccattcg agtattgctg ctatggcact
    14521 ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg acacgcgcgg
    14581 ttttcctgat tttaaacttc atctggcaca gagtctggcg aatggcactc cctatgttaa
    14641 tcgcaatgtc aatgaagatg acagcgttga atcatacaca gggaaaatat ttgagtccgc
    14701 aatggcaacg cttgatcatg tccgtcattc actggataaa tctgccatca accgcgccgt
    14761 cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga ttaggctctt cagccgccgt
    14821 tgcccacgat gcgatgaata agttctttcg ttttaatgtt ccggtggtgt actccgatga
    14881 tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg tggtgctgat
    14941 ttctcacact ggaagaacaa aaaatctggt cgagctggcg cagctggctc gcgaaaacga
    15001 cgcaatggtg attgccctca cctctgcggg taccccgctc gcccgggaag caacgctggc
    15061 aattaccctc gacgtaccgg aagatactga catttatatg cccatggttt ctcgacttgc
    15121 acagctgacc gtgatagatg tgctggcgac aggatttact ttgcgacgcg gtgcaaaatt
    15181 cagagataac ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg ataagcagtt
    15241 acttaattta agtgacgatc gctaaaaacg actgtcactg tcctaatctt atacgacatc
    15301 cgaatgagat taatttatcg ccatcgcggc gttatttcat tcggatttca tgttcaagca
    15361 acacctggtt gtttcagtca acggagtatt acatgtccag aaggcttcgc agaacaaaaa
    15421 tcgttaccac gttaggccca gcaacagatc gcgataataa tcttgaaaaa gttatcgcgg
    15481 cgggtgccaa cgttgtacgt atgaactttt ctcacggctc gcctgaagat cacaaaatgc
    15541 gcgcggataa agttcgtgag attgccgcaa aactggggcg tcatgtggct attctgggtg
    15601 acctccaggg gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt ttcctcaata
    15661 ttggggataa attcctgctc gacgccaacc tgggtaaagg tgaaggcgac aaagaaaaag
    15721 tcggtatcga ctacaaaggc ctgcctgctg acgtcgtgcc tggtgacatc ctgctgctgg
    15781 acgatggtcg cgtccagtta aaagtactgg aagttcaggg catgaaagtg ttcaccgaag
    15841 tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa caaacttggc ggcggtttgt
    15901 cggctgaagc gctgaccgaa aaagacaaag cagacattaa gactgcggcg ttgattggcg
    15961 tagattacct ggctgtctcc ttcccacgct gtggcgaaga tctgaactat gcccgtcgcc
    16021 tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt gcggaagccg
    16081 tttgcagcca ggatgcaatg gatgacatca tcctcgcctc tgacgtggta atggttgcac
    16141 gtggcgacct cggtgtggaa attggcgacc cggaactggt cggcattcag aaagcgttga
    16201 tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc gacccagatg atggagtcaa
    16261 tgattactaa cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac gccgttctgg
    16321 atggtactga cgctgtgatg ctgtctgcag aaactgccgc agggcagtat ccgtcagaaa
    16381 ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa aatcccgagc atcaacgttt
    16441 ctaaacaccg tctggacgtt cagttcgaca atgtggaaga agctattgcc atgtcagcaa
    16501 tgtacgcagc taaccacctg aaaggcgtta cggcgattat taccatgacc gaatcgggtc
    16561 gtaccgcgct gatgacctcc cgtatcagct ctggtctacc aattttcgcc atgtcgcgcc
    16621 atgaacgtac gctgaacctg actgctctct atcgtggcgt tacgccggtg cactttgata
    16681 gcgctaatga cggcgtagca gctgccagcg aagcggttaa tctgctgcgc gataaaggtt
    16741 acttgatgtc tggtgacctg gtgattgtca cccagggcga cgtgatgagt accgtgggtt
    16801 ctactaatac cacgcgtatt ttaacggtag agtaatctca cctgccccgg ccgctgatgg
    16861 ccggggtatt tagcaacaca taaattagga ctacttattc aatccaatca taatcacagc
    16921 cccctgtaaa atgaatacag ggatagcctg aatggagatc taaatagctt gcaagacgtc
    16981 gcatacccgc ttcctctgca acactatgtc caataactgc catcggtaag ccactatctt
    17041 tggagtattg tgtctgcacc catgttttaa ttgaatcgtt aatcgtaata taataatcag
    17101 cctgatattc catatattgt attggatcac aaaaacagcc tgccccaatg ccaacagttc
    17161 tgactactct ggctttatct ccataaaatt gaatgccagg aagattatat attttcaaat
    17221 ttttggcaaa acgtttggta atgttaatgg cactgtcagg gtctatagca tatacacagt
    17281 acatctcatc agaagcaaca atatttgtaa catccagttc taactgctga gccaaagcct
    17341 tagacattcc gaacgtaggc tctcgatcca tgacatcatg gcaacgaatg attaccaaat
    17401 tatttttatg caaaaactcc tttttttgct caattatttt taaataactg agttcaccgc
    17461 gagtataatc cattattttt ctggcccatg aaaactcaag atcgtcaccc tccagatcag
    17521 cataagaata aaatgtaggc tcatgtgtaa taatgatatt gacaccttca aaaacagcct
    17581 ttcttaaaac atccattgag gcaatccagc aggtcccaat tttattaata agtgtcgcct
    17641 cagcaccaaa aataatttta tcaacagttg gttgcgaaac agcaaccatt gaatttaaaa
    17701 aacaattaag atccgcggct gttttaatat gatgcattca acatccttca caggtttcat
    17761 cagcaataaa ataatttcaa catttctggg aaacgttttg cccatgcaga ctcagagtgg
    17821 gtatcacctc cccataaatg
//
LOCUS       DAJVXP010000081        17738 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVXP010000081 DAJVXP010000000
VERSION     DAJVXP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.081"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..183)
                     /locus_tag="OQS10_002622"
     CDS             complement(<1..183)
                     /locus_tag="OQS10_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2293625.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLT"
     gene            complement(393..911)
                     /locus_tag="OQS10_002623"
     CDS             complement(393..911)
                     /locus_tag="OQS10_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS2293626.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1608..2108
                     /gene="tssB"
                     /locus_tag="OQS10_002624"
     CDS             1608..2108
                     /gene="tssB"
                     /locus_tag="OQS10_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCS2293627.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2143..2367
                     /locus_tag="OQS10_002625"
     CDS             2143..2367
                     /locus_tag="OQS10_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293628.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2418..3893
                     /gene="tssC"
                     /locus_tag="OQS10_002626"
                     /pseudo
     CDS             2418..3893
                     /gene="tssC"
                     /locus_tag="OQS10_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            3900..4313
                     /gene="tssE"
                     /locus_tag="OQS10_002627"
     CDS             3900..4313
                     /gene="tssE"
                     /locus_tag="OQS10_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS2293629.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4317..6167
                     /gene="tssF"
                     /locus_tag="OQS10_002628"
     CDS             4317..6167
                     /gene="tssF"
                     /locus_tag="OQS10_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS2293630.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6131..7213
                     /gene="tssG"
                     /locus_tag="OQS10_002629"
     CDS             6131..7213
                     /gene="tssG"
                     /locus_tag="OQS10_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCS2293631.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7238..8518
                     /gene="tagH"
                     /locus_tag="OQS10_002630"
     CDS             7238..8518
                     /gene="tagH"
                     /locus_tag="OQS10_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCS2293632.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8515..9039
                     /gene="tssJ"
                     /locus_tag="OQS10_002631"
     CDS             8515..9039
                     /gene="tssJ"
                     /locus_tag="OQS10_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCS2293633.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9042..10371
                     /gene="tssK"
                     /locus_tag="OQS10_002632"
                     /pseudo
     CDS             9042..10371
                     /gene="tssK"
                     /locus_tag="OQS10_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10376..11137
                     /gene="icmH"
                     /locus_tag="OQS10_002633"
     CDS             10376..11137
                     /gene="icmH"
                     /locus_tag="OQS10_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCS2293634.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11146..13935
                     /gene="tssH"
                     /locus_tag="OQS10_002634"
                     /pseudo
     CDS             11146..13935
                     /gene="tssH"
                     /locus_tag="OQS10_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            13932..14675
                     /gene="tagO"
                     /locus_tag="OQS10_002635"
     CDS             13932..14675
                     /gene="tagO"
                     /locus_tag="OQS10_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCS2293635.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14680..16092
                     /gene="tssA"
                     /locus_tag="OQS10_002636"
     CDS             14680..16092
                     /gene="tssA"
                     /locus_tag="OQS10_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCS2293636.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16111..>17738
                     /gene="tssM"
                     /locus_tag="OQS10_002637"
     CDS             16111..>17738
                     /gene="tssM"
                     /locus_tag="OQS10_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCS2293637.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4135 a         4854 c         4854 g         3895 t
ORIGIN      
        1 cgtcaggtag gccattttgt ccagtacctg cggaaattca agggagagaa actgctggct
       61 gaccagagag agctcgaggg aaaagagcga agagagtgac tggttcagat gaaaggagac
      121 caccgcaaaa gcatccggtg gcaggccgtc cacttccagc gtgaaacgta atccggttga
      181 catagcaacc tccggggtta aggaaaaaca gacagttcgc tgttaaagcg gctgtgacag
      241 tacagtgtta cgcttgcgtt caggcacgct accgccgaag tttccggcat ccgccatctc
      301 ccgtgaacgt aaccccttac tgacaacctt tctttctacg gcgtaataca aaaacagcat
      361 ccggcctgat agccggatgc tgttggctta acttatgctt ccagtggtgc gcgccagtcg
      421 tcggcaccgg aggtgccaga tttgacgtgt tcccattcaa ttttgcggta ggccagtgaa
      481 acctcgagca actgggtgaa ctcgcgtttc gccgggtcct ggcaatgcgg catatgcagt
      541 ttcatatcga caatcgtcga atcagtcagg cgggtagtga aataatgctc ctgtttgccc
      601 tccacagagg tacgccacca gtgaagttca gtggttggca gcatttcgcc ggaggccagt
      661 gcgttgtaca tcagcggaac cgctttgttc agcgccacgg taaaaatgaa aggcttgtga
      721 gcacgctgac ccgcaggctg accggactga ggagtggtcg ggacagtaac gttatgcagg
      781 aattcctgca ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc gacagaatca
      841 gcggtaaatg cacctgcagt aatatttccc tgggtctgac cagtgatgga gatataacac
      901 ggtgttggca tttgtaaact ccttgttaac gtggttaatt actacttaca atgacctgcc
      961 gttaatccca gacaggcaga tgcagctcac ttcacggaac cgcatcattt ttcagtattt
     1021 tttacataca aaccgagcta gaatttacgg ttgtcgtaaa aactattttt aaaatcatta
     1081 aatctcttat tcagcaataa cttaataata tatatctgga taaccatacc caactgttca
     1141 aagtccgtat tgcctccata aaatggccaa agctgaatca tgaataaata ttgtatttat
     1201 cctttctgaa ttatcatttt gataattttt gagatattcc ttaaaaatac ccacttaatc
     1261 aatcacaaaa caaatatata aacaagcaat cattagatta gcttattttt aatgaaatta
     1321 ttatatactt caataagtta cacaaaaact ctatatttaa attattgcca taatacacct
     1381 gaagcgaatt gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg
     1441 atcgataaaa agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag
     1501 tggtgatttt attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa
     1561 tatgccgtgt tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat
     1621 ttgaaggcag cgtcgcgccc cgcgagcgta ttaatatatc gtatgtccca aaaacagatg
     1681 gccagacggc tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata
     1741 cgcaggaaac atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg
     1801 gtgcagtgat ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg
     1861 gaagcacaac agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt
     1921 caccagatag cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag
     1981 cactgactgc actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg
     2041 cattgctgga aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca
     2101 ataaataacc accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat
     2161 atctccggga cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt
     2221 ttcagcccgt ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat
     2281 cgccgtatca gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg
     2341 tcgcactggc agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa
     2401 acagaaggat tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc
     2461 agcctgagca ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg
     2521 gttcggaagg ttatgacgtt gccctccagg gtgtgacagc ctttattgcc agcatcctgc
     2581 aatccactgc atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata
     2641 ttgatgaacg catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg
     2701 tcgagtcgtt ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca
     2761 tcaaggtcaa cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc
     2821 cagaaattat tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg
     2881 gcggtaaacc catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca
     2941 tgaaactgct gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct
     3001 ccgtttcccc ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc
     3061 tttatgccat ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag
     3121 attcgcgcta tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa
     3181 cagataaccc tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt
     3241 atctgtgggg taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat
     3301 accgctgatg tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccga
     3361 tgcatctgtt cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca
     3421 ccgatcgccg cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag
     3481 actctgataa tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg
     3541 gaaaagatgc agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca
     3601 atcggttagc gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa
     3661 ggagcgactt agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa
     3721 acccgcctgc tgacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg
     3781 atgtagaagg cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat
     3841 ttatgggggc aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca
     3901 tgataccgga tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg
     3961 ccagaaaccg gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac
     4021 gaaacattct taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg
     4081 aaccggaagg ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag
     4141 aacgctatga accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca
     4201 gcccgctgga tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg
     4261 tgcttgaatt tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg
     4321 aatttgaaga acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc
     4381 tggcaacgga aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata
     4441 ttgaacgcct gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg
     4501 atgaattccc agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc
     4561 cggttccggc aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg
     4621 tgttaatccc ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag
     4681 tgctgccctc tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg
     4741 atatctggct gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg
     4801 gtgttatcaa catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga
     4861 acaaacttcg cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat
     4921 ggttctgcga atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc
     4981 ctgagtttat gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga
     5041 aaaacgtcca cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc
     5101 tcttttttga tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta
     5161 ccctgcaact gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc
     5221 tgcgcctgta ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc
     5281 tggacaaccg gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg
     5341 atgtattttc cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc
     5401 tggggcgtcc tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc
     5461 atcagataga atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat
     5521 ccctgttcca tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc
     5581 cgtcagacga atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc
     5641 gtgagcttcc gtcacaaatt cgttccggcg atatcaccgg cacaaccgga aaaaatgcgg
     5701 ctgttgcttc atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg
     5761 gcagcctgca ctggccccta ctctccgcca tgaacctgaa ttatctgtca ttactggata
     5821 cggacgcgct gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga
     5881 cggcacggct gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg
     5941 atcgcctgtt tacgggtatt cccgtccggg ggctggcttc cacgctgtat ctgcacccgg
     6001 agccgtttgt ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt
     6061 cgctgtacgc cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg
     6121 agacatggaa atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc
     6181 cccggcagat aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta
     6241 caattttttt gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc
     6301 agaattatcc ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag
     6361 ccttgccttc ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag
     6421 gatgaccacc acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct
     6481 cgctcacctg gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat
     6541 gttcagccac cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat
     6601 cagttttcgt aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct
     6661 gggccaccgc cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc
     6721 cgggatcctg gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg
     6781 ctttgatttg tcagaagtga cactgcaaaa ctggcaacgt cgcaaggtgg atattgaacc
     6841 ggatcagcaa aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag
     6901 atcggtactg ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca
     6961 gctcagtatt accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa
     7021 cttcctgcca ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt
     7081 acatctgggg ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc
     7141 gctgctgggc tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag
     7201 ggtacggtcg taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac
     7261 gctttcattg caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct
     7321 gttcacacag cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg
     7381 tcagcagagc attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct
     7441 gcgcccgcag acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct
     7501 aatacagttg cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct
     7561 gaaccgggga gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac
     7621 caatcgcgat atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg
     7681 aatgactcac cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact
     7741 ccaggctctt cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc
     7801 tgtccggcca tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt
     7861 tatggatctg ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc
     7921 agaaatggca caacgccacg ttgcggtcac ccccttactg cgcggtcttg gcggctcgct
     7981 taccatgagc aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca
     8041 ggccgcaata aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca
     8101 tttgcgcccg ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga
     8161 cgtaatgttt gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga
     8221 aagcctgcgc aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct
     8281 tcgtgtcctg ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg
     8341 ccgcagccat gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca
     8401 ttattacgaa gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt
     8461 ctacgcccag gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac
     8521 aggaaggcgt ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg
     8581 aaaaaaatca gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa
     8641 ccttcggaaa taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt
     8701 gaatcagccg ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca
     8761 gccgactatg accagatagc cagcacgccg ctaccggatg tgctgggtaa aaactatatc
     8821 gaccaccagg atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg
     8881 gatgagaaaa cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa
     8941 tggaaacaaa tcgagacggt ggaaggaatc ggccaccatt accgcctgct ggtgcatgta
     9001 cgtcaaagca gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca
     9061 aggtgatgtg gcaggaaggg atgctgatgc gtccacacca tttccagcag cagcagcgtt
     9121 acaacgacta cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta
     9181 ccgaactcac cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt
     9241 caggcacact gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc
     9301 cgctgcaccc gcaaaatttt ccggacgaaa gaagccgcaa tatctacctc gctctgccgg
     9361 tcgccagtga tgtgagaaat gaaatcagcg acggacggcg aatcgggcgt taccggctga
     9421 attatgccga tgtccgggat ttgcattcag aagaaggtga cgcgcgaaca ttgacgctgg
     9481 gtcaactgac gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc
     9541 cgctttgccg tatcagcgat cgccgccgat ggctccctga cgttggatga cgattttatc
     9601 ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag
     9661 ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc
     9721 ggcatcgcgg atgtggcgga atttatgatg ttgcagttac ttaaccgtaa ccagacccgg
     9781 tttacccatc acgcccgtcg atcccagctc cacccggaag atttctacct tgatcttgcc
     9841 gggttgctgg gtgaactgat gacctttacc gagccgtcgc gcctgccctg cccgcttgat
     9901 gtgtatgatc atcatgacct gaccaaaata tttaaaacgc tgttaccgga agtcaaacgg
     9961 gcgctgcata ccgtactgtc gccaagagcc gtcaatctgc cgctgcatct gcgtgacggt
    10021 atctggcagg ccgatatcca tgacacagaa ctgctgcaat ctgccacctt tgtgctggcc
    10081 gtggcggcaa acatgccggt cgatcagatc cagcgccagt ttatccagca gtcgaaaatt
    10141 tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tccccggtat tccattgcgt
    10201 gcccttatgg tggctccccg ccagcttcct taccattccg ggttcagcta tttcgaactc
    10261 gacaagagtg gccaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgtt
    10321 tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga
    10381 tgaggggtca ctgtctctgc caccatttac cggacacgac gaaaaaagcc agcgcaacta
    10441 ccatctcgcg cttcgtggca acagcctgaa tccgatgatc gacgcggcca cgccgctact
    10501 cggtatggta atgcgccttt caaccatgaa cagccagacg atgccggagc atctttttgc
    10561 tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggagcagg gctacgaacc
    10621 gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg
    10681 taacggctgg tcaaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa
    10741 cgaagcctgg ggtggcgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa
    10801 acgttaccag gatctgctgg aatttctctg gatctgtttt tctctgggat ttcgcgggcg
    10861 ctacaaagtg gcagcacagg atcaggggga atttgagcag atctaccgcc gtctgtacca
    10921 cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac
    10981 ccagggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt tttgtggtgg
    11041 cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca
    11101 ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat ccagattgat
    11161 cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc
    11221 tctgaatgta tgagtcagca ggcagcggaa attaccgtca gccatgtgct cattcagatg
    11281 ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat tggcatggaa
    11341 gagttgcgcc aggcgctgac ggtagagaac tacacaaccg cccgttctgc ggacagctac
    11401 ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctagctgct ggcgtcggct
    11461 gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccctgct gcattcgccg
    11521 ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg
    11581 caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac
    11641 ggtaaaggcg caggcacaat gacggacgcc tctgacaccc tgcttgcccg ctatgccaaa
    11701 aacatgaccg cagacgcccg taacggcagg cttgacccgg tactgtgccg cgaccacgaa
    11761 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccagt ggtggtgggc
    11821 gaagcgggcg tgggcaaaag cgcactgatt gaagggctgg cgctgcgcat cgtggcaggc
    11881 caggtgccgg acaagctgaa aaacaccgat atcatgaccc ttgacttggg cgcattgcag
    11941 gccggggcgt cggtgaaggg tgaattcgaa aaacgtttca aagggctgat ggcggaggtc
    12001 atttcctccc cggtgccggt cattctgttt atcgacgaag cacataccct gattggcgcg
    12061 ggcaaccagc agggcgggct ggatatctcc aacctgctca aaccggcgct ggcgcgcggc
    12121 gagctgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgaaaaagat
    12181 gccgccctgt cgcgccgctt ccagttggtg aaggtcagcg aacccaacgc tgccgaagcc
    12241 accattattc tgcgcggtct gtcggcggtc tatgaacagt ctcacggcgt gctgattgat
    12301 gatgacgcct tgcaggccgc tgcgacatta agcgagcgtt atctctccgg gcgtcagtta
    12361 ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtcg
    12421 tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga ggcggaaatt
    12481 cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag
    12541 gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc ggcctggcac
    12601 cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact gggcgtggca
    12661 gaggacgatg cggcgccgtt gccggacgca gataccgtgg aggatacgca gccagagtca
    12721 gagtcagagt cagagtcaga gtcagagtca gagcaggata ataccggtgc cgaaccggct
    12781 gatgaggccg acagagaaca accggaagag accgctgaaa cagtttcccc ggtacagcgg
    12841 ctggcccagc tcactgccga actggacgcc ctgcataacg accggttgct ggtctccccg
    12901 cacgtcgata aaaaacagat tgcggcggtg attgccgaat ggaccggcgt gccgctcaac
    12961 cgcctgtcgc agaatgaaat gtcggtcatc accgacctga cgaaatggct gggtgacacc
    13021 atcaaaggcc aggacctggc gattgccagc ctgcataagc atctactgac cgcacgcgcc
    13081 gacctgcgtc gtccgggacg cccgctcggt gcgttcctgc tggctggccc cagcggtgtg
    13141 ggtaaaaccg aaaccgtcct gcaactggca gagctgctct acggcggtcg ccagtacctg
    13201 accaccatca atatgtccga attccaggag aaacacaccg tctcgcggct gattggttcg
    13261 cctccgggct acgttggcta cggtgaaggc ggcgtactga ccgaagcgat tcgccagaaa
    13321 ccctactcgg tagtactgct cgatgaagtg gaaaaagcgc acccggatgt gctcaacctg
    13381 ttctaccagg cgttcgataa gggcgaaatg gcagacggtg aaggccgcct gattgactgc
    13441 aaaaatatcg tcttcttcct gacgtccaac ctcggctacc aggtaatagt cgagcatgcc
    13501 gatgacccgg aaaccatgca ggaagtacag tatccggtgc tggccgactt cttcaaacct
    13561 gccctgctgg cgcgtatgga agtggtgccg tatctgccgc tgtcgaaaga gacgctcgcc
    13621 accattatcg ccggaaaact ggcccgtctg gataacgtgc tgcgcagtcg ctttggtgta
    13681 gaagtggtca ttgaaccgga agtgacggac gaaatcatga gccgcgtcac ccgcgcggaa
    13741 aacggcgcga ggatgctgga atcggtcatc gatggcgaca tgctaccgcc gctctcgctg
    13801 ctgctgttgc agaaaatggc ggctaacacg gcgattgccc ggattcggtt gtcggcagtg
    13861 gacggcgcat ttacggcaga cgtggaagat gctcagaacg acgagtccgt cacaaaggat
    13921 gaaacggttt tatgagcagg ttgcgcgttt tccggcagac aggatggttt atcgcgggcc
    13981 tgatgacggg cttacccgcc actgccgcgc ccgcagaagc atcgtcaatg gccggtgtgg
    14041 cggtcgccgt cgccaccacg accccgccgg atgcgacagc cacgttacag gccatgcagt
    14101 cctgtcggcg ggagtccgcg gcgctggagc ggcttgactg ctatgaccgc ctgctggcac
    14161 cgctgtcccc gtcagggttt gacggggcgc tggtcaaagc cggttttgtg ggcgaggcct
    14221 ggacccgcgc cacagagcag gaaaaacgcc gcgagggcaa caccacagaa ctgctggtga
    14281 cgcaggtgcc gggggagcgc ccgacggtgg tgataaccac cccggctatc ggtcatgttc
    14341 cgccacgccc ggtgctgatg ttcagttgcg tggacaatat cacccggatg caggtggcgc
    14401 tgatgcaccc tctggatgtc catgatatcg ccgtcactct gaatgccgac aaccgggcat
    14461 tgcgcagcca ctggtttgtg cgcgagaacg gcaccctgct ggagtccagc cgggggttat
    14521 ccgggattga tgaaattaag caactgttcg gcgcaaaaac gctgacggtg gataccggcg
    14581 cagacaatgc cgccggaaag ctgaccttta acattgacgg gctggcaagg gcgattgctc
    14641 ccctgcgcga tgcctgccac tgggcgggag aataaaaata tggcaatgga cttacgcgat
    14701 ccgaatgtct ggatatcgca cctgctggaa aacctgccgg aagagaaact ggcatcggcg
    14761 ctgaaagatg acaacccgaa ctgggagtat atcgacggcg aaatcgtcaa gctgggatcg
    14821 ctggcccaca gtcaactgga tatcccggaa ctccagcgcc gggggctggt cattctggct
    14881 tcggagagta aggattttcg tctgctggcg catctgctaa gaaccctgca acacgccggt
    14941 gatcctcatc tcgcattgcg cctgctggcg ctgtatgtgg agcattactg ggcggtggct
    15001 gcgccgcaga atatggcgca taaaaaacgc tttgccagcc aggtcattaa acgctttgaa
    15061 acaggtattg aagttttttc acagaacgcc gccacagcgc agcgcgatgc gctgtcaggg
    15121 gaactggcaa aactggcgca atgctggcag tcacataacg ccccggaact ggcacaggct
    15181 actgatgatc tttttgccct gtaccagcgt gcgtttaatc gtgcggcacc ggctcctgtc
    15241 cccactccgg cggcctccgg tagttcacca cagaccaccg ccacgtctga aagcggcgtg
    15301 acgcaaccca gtgctccggc tccccaaatc gtcatcgaca gtcacgacga caaagcctgg
    15361 cgcgacacgc tgttaaaagt ggcggccatt ttatgtgaac gccagccgga ttcaccgcag
    15421 ggctaccgtc tgcgccgcca tgcgctttgg cagaacatca ccagtacgcc ccaggcggaa
    15481 agcgatagcc gtactccact ggctgcggtc tccgccgata tggtggcaga ttaccatgct
    15541 cagctcggta gtgccgatat ggcgctgtgg cagcaggtgg agaaaagcgt actgctggct
    15601 ccttactggc tggatggtca ctgcctttct gcacagacgg cgctgcgtct gggttacaaa
    15661 caggtggcag acgccatcca cgatgaggtc atccgcttcc ttgagcgtct gccccagctt
    15721 accgggctgc tgtttaatga ccacacaccg tttatcagtg agcagacgaa acaatggctg
    15781 gcagcttcgc ccgacgccaa agttgcaccg gttgcgcaaa tcggtgagga atcgaaggca
    15841 gccagagcct gttttgctga gcagggtctg gaggcggcgc tgcgatatct ggacatgcta
    15901 cccgaaggcg atccccgcga tcagtttcac cgccagtacc ttgccgcaca gttgacggaa
    15961 gaggcggggc tggtacagct tgcgcagcag cagtaccgga tgttgttcag gatgggattg
    16021 cagatgatgg tggcagactg ggagccgtcc ctgcttgagc agctagaaca aaaattcacg
    16081 gcagaacaat aaaagagaac aggagttttt gtgttcagat taccaacacc ccgactactc
    16141 agcggactca aatcagccct gcgaccggcg atgccccggt ttaaagtctc tgctttctgg
    16201 ctgctgatac tggcgtggat ttttctgctg gtgtggatct ggtggaaagg cccgatgtgg
    16261 acgctgtatg aagaacagtg gctcaaacca ctggcgaacc gctggctggc aacggcggcg
    16321 tgggggatta ttgccctggt gtggcttacc gtccgggtga tgaagcgcct gcaacagctt
    16381 gaaaaaatgc aaaagcaaca gcgcgaggaa gccgttgatc cgctcagcgt ggaactgaac
    16441 gcccagcagc gttatcttga ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc
    16501 cgtttcctgt ggcagttgcc gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg
    16561 acactgctgc gcgaggggtt tccgtccgac attatttatg ccccggaggg cgcacgcggc
    16621 gcagaacaac gcctgtacct cacgccccat gtcggtaaac aggcggtgat ctttgatatc
    16681 gacggcacac tctgcgctcc cgctgatgcg gatatcctgc atcgccgtct gtgggaacat
    16741 gccctcggct ggctgaaaga aaagcgcgcg cgccagccgc tgaacgggat tattctgaca
    16801 ctcgatttac ccgatctgct gaccgcagac aaacgccgcc gcgagcatct gttacagacg
    16861 ctgcgcagcc gcttacagga tatacgccag catcttcact gccagttacc ggtttacgtg
    16921 gtactgaccc ggcttgattt attacagggt tttgccgccc tgttccagtc actcaacaga
    16981 caggatcgcg atgcgattct gggcgtcacg ttcacccgcc gtgcccatga aaatgatgac
    17041 tggcgaacag agttgaatgc tttctggcag acatgggtgg atcgaatgaa tctggcgttg
    17101 ccggatctga tggtcgctca gactcacacc cgcgcgtctt tattcagttt ttcccgccag
    17161 atgcagggaa gccgtgaacc gctggtgtca ctgcttgagg gtctgcttga tggcgaaaat
    17221 atgaacgtga tgctgcgtgg tgtctatctc acctcttcgc ttcagcgtgg acagatggat
    17281 gatatattca cccagtctgc cgcccgccag taccggctgg gcaataaccc actggcgtcc
    17341 tggcccctgg tggacaccgc gccttatttc acccgcagcc tgttcccgca ggcattactc
    17401 gcagagccta atctggcaac agagagccgc gcctggctga tacgttcccg tcgccgcctg
    17461 acggttttct ctgccacagg cggcgtggca gcactgctgc tcatcaccgg ctggcatcac
    17521 tattacaacg gtaactatca gtccggcatc accgtgctta agcaggccaa agcctttatg
    17581 gacgtgccgc ctccgcaggg ggaagatgac tttggcaacc tgcaactgcc gctcctgaac
    17641 ccggtacgcg atgccacact ggcctatggc gactggggcg accgcagccg tctggccgat
    17701 atgggactgt accagggacg acgtatcggg ccttatgt
//
LOCUS       DAJVXP010000082        17647 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVXP010000082 DAJVXP010000000
VERSION     DAJVXP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.082"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..893)
                     /gene="ydiK"
                     /locus_tag="OQS10_002638"
     CDS             complement(<1..893)
                     /gene="ydiK"
                     /locus_tag="OQS10_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS2293638.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRP"
     gene            1282..4338
                     /locus_tag="OQS10_002639"
     CDS             1282..4338
                     /locus_tag="OQS10_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS2293639.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4335..4745
                     /gene="menI"
                     /locus_tag="OQS10_002640"
     CDS             4335..4745
                     /gene="menI"
                     /locus_tag="OQS10_002640"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS2293640.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            4845..5033
                     /locus_tag="OQS10_002641"
     CDS             4845..5033
                     /locus_tag="OQS10_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS2293641.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5581..5949
                     /gene="sufA"
                     /locus_tag="OQS10_002642"
     CDS             5581..5949
                     /gene="sufA"
                     /locus_tag="OQS10_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS2293642.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            5958..7445
                     /gene="sufB"
                     /locus_tag="OQS10_002643"
     CDS             5958..7445
                     /gene="sufB"
                     /locus_tag="OQS10_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS2293643.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7455..8201
                     /gene="sufC"
                     /locus_tag="OQS10_002644"
     CDS             7455..8201
                     /gene="sufC"
                     /locus_tag="OQS10_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS2293644.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8176..9447
                     /gene="sufD"
                     /locus_tag="OQS10_002645"
     CDS             8176..9447
                     /gene="sufD"
                     /locus_tag="OQS10_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS2293645.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9444..10664
                     /gene="sufS"
                     /locus_tag="OQS10_002646"
     CDS             9444..10664
                     /gene="sufS"
                     /locus_tag="OQS10_002646"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS2293646.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10677..11093
                     /gene="sufE"
                     /locus_tag="OQS10_002647"
     CDS             10677..11093
                     /gene="sufE"
                     /locus_tag="OQS10_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS2293647.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11242..12246
                     /gene="ldtE"
                     /locus_tag="OQS10_002648"
     CDS             11242..12246
                     /gene="ldtE"
                     /locus_tag="OQS10_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS2293648.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12309..12545)
                     /gene="lpp"
                     /locus_tag="OQS10_002649"
     CDS             complement(12309..12545)
                     /gene="lpp"
                     /locus_tag="OQS10_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS2293649.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(12855..14267)
                     /gene="pykF"
                     /locus_tag="OQS10_002650"
     CDS             complement(12855..14267)
                     /gene="pykF"
                     /locus_tag="OQS10_002650"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS2293650.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            14824..15033
                     /gene="fumD"
                     /locus_tag="OQS10_002651"
     CDS             14824..15033
                     /gene="fumD"
                     /locus_tag="OQS10_002651"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS2293651.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            15489..16115
                     /locus_tag="OQS10_002652"
     CDS             15489..16115
                     /locus_tag="OQS10_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS2293652.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            16136..>17647
                     /locus_tag="OQS10_002653"
     CDS             16136..>17647
                     /locus_tag="OQS10_002653"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS2293653.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFI"
BASE COUNT         4488 a         4177 c         4645 g         4337 t
ORIGIN      
        1 attgggcgga tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt
       61 ccccaggtgg tatcgccggt ccagtagagc cagataatcg ccggaatcag taccggcaac
      121 gggccaagct ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt
      181 acgccggata cggcgaggcc gatactgcca agcactgcct gtactaacgc cgtcaccacc
      241 acacccagcg ccaccgcgcg gatagcctgt gccgccagca gcacggcggc atctccgcga
      301 acgcctgcca gacgggttgc aaaatggcga atgccttgtg ccacctgttc accgcgccag
      361 tacagcaggg cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc
      421 gccgcctgcc caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg
      481 atcgccgtac cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc
      541 accggaatgg tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct
      601 ttaattagcg ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata
      661 aacaccatca ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg
      721 atcttttgca aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca
      781 aagccgagaa taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac
      841 agcaccgaaa gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact
      901 tttcaccttt tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca
      961 taatgatgag caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc
     1021 gtgttagaaa aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta
     1081 tttcaagttg catgcgctgc ggctacgttc gttcacccca gtcacttact attgtaagct
     1141 cctggagttc actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat
     1201 cacctttcag tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga
     1261 ccgcaggaaa gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa
     1321 ctggtgctta attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca
     1381 agttatgccg atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat
     1441 gcggtggtat ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag
     1501 gaacgctatt catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag
     1561 gcgctcaacc aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt
     1621 aaccctgaag agggctgggt gcgcgttgag gccggggtga taaaagatca actcaatcag
     1681 tacctgaaac cgttcggcta cttttttgca ccagaacttt cgaccagcaa ccgggcaacg
     1741 ctcggcggga tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg
     1801 tcagatcacg tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa
     1861 cctttacccg tcgaactggc ggaaacgctg ggtaaaacca ataccacaat cgggcgaatt
     1921 tataacacgg tttatcaacg ttgccgtctg caacgccagt taatcatcga caacttcccc
     1981 aaacttaacc gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc
     2041 gagttcgacc tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa
     2101 gcgcggctgg atattacgcc cttgcctaaa gtgcgccgtc tggtgaacgt caaatatgac
     2161 tcttttgact ccgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta
     2221 gagacggtgg actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc
     2281 agcgagttga ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggaattt
     2341 gctggtgatg atgaggcgct gattgacgag cgagttaatg cactctgtgt gcggcttgat
     2401 gagctgattg ccagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg
     2461 ggggttgaac gtatctatgc aatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa
     2521 ggtgccgcta agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc
     2581 gattatattg ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc
     2641 ggtcacgtcg acgcaggtgt tttgcacgtc cgtccggcac tggatatgtg cgatccgcaa
     2701 caagagattt tgatgaagca aatctctgat gacgtggtgg cgctgactgc gaaatacggt
     2761 ggtttgttgt ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc
     2821 ggtgaggaac tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga
     2881 ctcaacccag ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac
     2941 gcggtgaagc gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc
     3001 ggtgcgatgg agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg
     3061 tgtccgtcga tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg
     3121 gtgcgtgaat ggctgcgttt gttggcggat cgcggcgttg atccactcaa actggaacaa
     3181 gaactgcctg aatccggcgt cagtttacgg acgttaattg cccgcacgcg caatagctgg
     3241 catgcgaata aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt
     3301 ctggcctgta aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc
     3361 tctcgtttcc tgcagctcta tcacacccgt tatttacgcc cgctgcgcga ccacctcgtc
     3421 gctacggtcg agagctacgc accgctgatg gcacgcgcgc cgaaaacctt taacttcttc
     3481 attaaccagc cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgcca
     3541 ctgctgtcgg tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg
     3601 ctggaacagc ttgaagctct caatgcagag cagaaagcgc gcacggtgtt ggtggtgcag
     3661 gaccccttta ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggttgaa
     3721 aaattaggtt tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatc
     3781 aaaggctttc ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt
     3841 atggctaagc ttgggatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc
     3901 gatgaatata aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt acttgcgaat
     3961 gaatggctgg caagcgcact tgagtcacat ccagtggcta cagtcagcgg tgaatcatgg
     4021 tatttctttg gtcactgtac cgaggttacc gctttgccgg gcgcgccagc acaatgggcc
     4081 gcgatatttg cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg
     4141 gcagggactt acggacatga agcgaaaaac catgaaaatt cgctcgggat ctatgagtta
     4201 tcctggcatc aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc
     4261 tgccgtagcc aggtaaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta
     4321 ctggagatta ttaaatgata tggaaacgga aaatcaccct ggaagcactg aatgctatgg
     4381 gtgaaggaaa catggtgggg ttcctggata ttcgctttga acatattggt gatgacaccc
     4441 ttgaagcgac aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag
     4501 gagcatccgt ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag
     4561 gtgagcaaaa agtggttggt ctggaaatca atgctaatca cgtccgctcg gcacgagaag
     4621 ggcgggtgcg cggcatatgt aaaccgttgc atctcggttc gcgtcaccag gtctggcaga
     4681 ttgaaatctt cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt
     4741 tgtgacgttg tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta
     4801 aacggtggta tactgtgcac gtcttgtcta aaagcgagga tgctatgtct actcaacttg
     4861 atcccaccca gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc
     4921 aatatctcaa acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt
     4981 ctactgaatt aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac
     5041 ttaatgccgc aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt
     5101 cttaatactg cttatctacc gcctgatatt gttcaaaaat aatactaatc gcagtttgtg
     5161 ttaaaacggc gggttagctt tatgagatta ttcttatcgc cccttcaaga gctaagccac
     5221 tgagagtgcc ggagataagc gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga
     5281 cttaagggtt ttcttatttc tgaacgctgc acttttttac ctatctctta aatagtcatt
     5341 ttcaatacaa ctttttcttt ttcatcacag ccttaatgct ccttagataa taaccattat
     5401 ctaacaatga gatacctaat tcttagcgtg cctgttaacc cacaaatcag ggtctatgct
     5461 tattaaaagc aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc tgggttgaac
     5521 tgataatcat tatcactagc atgctgttat acgctgaaag cgatgaagtg aggtaaatcg
     5581 atggacatgc attcaggaac ctttaaccca caagatttcg cctggcaagg cttaacgctg
     5641 acacccgcag cggcgataca catccgtgag ctggtggcaa agcagccggg tatggtcggc
     5701 gtgcgcttag gcgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt
     5761 agcgagccgg acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg
     5821 ctgcaagcga tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga aggacttaat
     5881 cagatattca aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt
     5941 ggggtatagg cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga
     6001 ccggcggccc gctgaattat aaagaaggat tcttcaccca gttagccacc gatgagctgg
     6061 caaaggggat aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt
     6121 ggatgctgga gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact
     6181 ggttgaaagc gcactacgac aagctgaatt atcaggatta cagttactac tcagcaccat
     6241 cgtgcggtaa ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg
     6301 cgaacgcctt tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc
     6361 gggaaggcaa agaggtggcg gtggatgcca ttttcgactc tgtttcggtt gccactactt
     6421 atcgcgaaaa actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg
     6481 atcatccgga actggtgcgt aaatatctcg gcactgtggt gccgggaaat gacaacttct
     6541 ttgccgcgct taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg
     6601 tgcgctgccc gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt
     6661 ttgagcgcac cattctggtg gccgacgaag acagctacgt cagctacatt gaaggctgtt
     6721 ccgctccggt gcgtgacagc tatcagttac acgcggcggt ggtggaagtc atcatccata
     6781 aaaacgccga ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg
     6841 gcggtattct caatttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt
     6901 catggacgca atcagaaacc gggtcagcga ttacgtggaa atatcccagc tgcattttgc
     6961 gcggcgataa ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag
     7021 cggataccgg caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga
     7081 aagggatctc tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga
     7141 cggcaaccaa tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg
     7201 gggcgcatac cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg
     7261 caacgacatc acgtattggt gaagatcaac tgttttactg cctgcaacgc gggattagcg
     7321 aagaagacgc catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc
     7381 cgctggaatt tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg
     7441 gataaggaat aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta
     7501 tcctgcgtgg attaagcctc gacgttcgtc ccggcgaagt tcacgccatt atggggccaa
     7561 acggttcggg caaaagtacc ttatcggcaa cgcttgccgg gcgagaagat tatgaagtga
     7621 cgggcggcac ggttgagttc aaaggcaaag atttgcttgc gctgtcgccg gaagatcgtg
     7681 caggcgaagg catctttatg gccttccagt atccggtgga gattcccggc gtcagtaacc
     7741 agtttttcct gcaaacggca cttaatgcgg tgcgcagcta tcgcggccag gaaacgctcg
     7801 accgctttga ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag
     7861 atttattaac ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata
     7921 ttttgcaaat ggcggtgctg gaaccggagt tatgcattct tgatgaatcg gactccgggc
     7981 tggatattga cgcattaaaa gtggttgccg atggcgtgaa ctcgctgcgt gacggcaagc
     8041 gctcattcat cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg
     8101 tccatgtgct atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac
     8161 tggaggagca gggttatggc tggcttaccg aacagcagta atgcgctgca acagtggcat
     8221 cacttgtttg aagctgaagg gacaaaacgc tcaccgcaag cacagcagca tttacaacaa
     8281 ttgctgcgta ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa
     8341 gggctgacca atagtcagtt tgtcagcatt gcgggagata tatccccaca gcagcgtgat
     8401 gccttagcgt taacgttaga cgccgtgcgg ctggtgtttg tcgatggacg ttacgtgccc
     8461 gcgctgagcg atgcaattga aggcagcgga tatgaagtga gcattaacga cgaccgtcag
     8521 gggttacccg acgctattca ggcggaagtg tttctgcatt tgacggaaag cctggcacaa
     8581 agcgtgacgc atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg
     8641 catatcaccc agggcgtggc aggtgaagag gtgaacactg cccattaccg gcatcatctg
     8701 gatctggcgg aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct
     8761 cgtcatttta ccggggcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat
     8821 atcaagctgg cgtttgaaaa cccgctcagt caccactttg ctcataacga tttgttgctg
     8881 gctgaggatg ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac
     8941 aacaccagta cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg
     9001 ccggtgaaaa acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt
     9061 aacagccgac agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt
     9121 ttgatcaacg tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg
     9181 ctgatgggca aaatggcgga agtggataca aaaccgcagc tggaaatcta tgcagatgat
     9241 gtgaaatgca gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg
     9301 cgctcgcgcg ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc
     9361 gaactgacgg aagcactgcg tgatgagggg cttaaacagc aggtgctggc ccgaatcggt
     9421 caacggctgc caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc
     9481 cggtgctttc tcgtgaggtg aacggtttgc cgctggctta tctcgatagc gccgccagtg
     9541 cgcagaaacc aagccaggtg attgacgccg aggctgagtt ttatcgtcac ggctacgcgg
     9601 cggtgcatcg cggtattcat accttaagcg cccaggcgac cgagaaaatg gagaacgtgc
     9661 gcaagcgggc atcgctgttt attaatgccc gttcggcgga agagctggtg ttcgtccgcg
     9721 gcacgacgga agggatcaat ctggtcgcca atagttgggg caacagcaac gtgcgggcgg
     9781 gcgataacat catcatcagt cagatggagc accacgctaa cattgttccc tggcagatgc
     9841 tttgcgcacg cgttggcgca gagctgcgtg tgatccctct caaccccgac ggtacgctgc
     9901 aactggagac gctgcctacg ctgtttgatg agaaaactcg cctgctggca attactcatg
     9961 tctccaacgt gctgggaaca gaaaatccgc tggcggaaat aatcacgctt gcgcaccagc
    10021 atggcgcaaa agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggatgttc
    10081 aggcgctgga ttgcgacttt tacgtgttct ccgggcataa actgtatggc cccaccggaa
    10141 ttggcattct ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg
    10201 gttctatgat cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccatggc
    10261 ggtttgaagc cggtacaccc aataccgggg gcattattgg tcttggcgcg gcgctggagt
    10321 atgtttcggc gctggggctt aataacatag ccgagtatga acagaatctg atgcattatg
    10381 cgctatcaca gctggaatct gtaccggatc tcactctcta tggcccacaa aacaggcttg
    10441 gcgttattgc ttttaatctc ggtaaacacc acgcctatga tgttggcagt tttctcgata
    10501 attacggcat tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca
    10561 acgtccctgc gatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggatc
    10621 gtctggtgac cggcctgcaa cgtattcacc gtttgctggg ataacaggga ggcactatgg
    10681 ctttattgcc ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aactgggaag
    10741 agaaatatct ctacattatt gagctgggcc agcgtctgac tgaattacgc gacgaagacc
    10801 gaagcccaca aaatagcatt cagggctgcc agagtcaggt gtggattgtc atgcgccaga
    10861 atgcgcacgg aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta
    10921 ttgcggtagt ctttattctc tacgatcaga tgacgccgca ggatattgtc agtttcgatg
    10981 tgcgtccgtg gtttgaaaaa atggcactca cccaacatct caccccatct cgttcacaag
    11041 gtctggaagc gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag
    11101 ggacagcttt catccggttg gctcacattg ccggatggtc aatgtttaca ctgacctgca
    11161 caggagtgtt ttggtttcag gtgaacataa gcgccatccg acgtttcagc gtgagtctcc
    11221 ggcaaataca ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc
    11281 gcttttagcg ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc
    11341 cgactggttg ggcaaaatca aacgtatacg gtgcaagaag gggataaaaa ccttcaggct
    11401 attgcccgac gttttgatac tgcggcaatg ttgatccttg aagccaataa cactatcgtc
    11461 ccggtgccaa aacctggtac gacgataact attccttcac aactgttatt acctgatgca
    11521 ccgcgtcagg ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga
    11581 gaaaatattg tgcaggttta tccaataggt attggattgc aggggctgga aacgccggtg
    11641 atggaaacgc gtgttgggca gaaaatccct aacccaacct ggacgcctac ggcaggcatt
    11701 cgtcagcgtt cgctggagcg tggcattaaa ttaccgccag tcgttcctgc cggaccaaat
    11761 aacccgctag gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat
    11821 ggcaccagtg cgccggacag cgttggtttg cgcgtcagtt cagggtgtat tcgcatgaat
    11881 gcaccggata ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc
    11941 aacgaaccgg tgaaatattc cgtggaacct aacgggatgc gttatgttga agtacatcga
    12001 ccactatcag cagaagaaca gcaaaacgtt cagacaatgc catacacact gccagcaggc
    12061 tttacgcaat ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat
    12121 cgtcgggcag ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg
    12181 ccttcagtag agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg
    12241 cagtagtggt aaatagcaga caaaaaaatg gcgcacaatg tgcgccattt ttcacttcac
    12301 aggtactatt acttgcggta tttagtagcc atgttgtcca gacgctggtt agcacgagct
    12361 gcgtcatctt tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt gctcagctgg
    12421 tcaactttag cgttcagagt ctgaacgtca gaagacagct gatcgatttt agcgttgctg
    12481 gagcaacctg ccagcagagt agaacccagg attaccgcgc ccagtaccag tttagtagct
    12541 ttcattatta ataccctcta gattgagtta atctccatgt agcgttacaa gtattacaca
    12601 aagtttttta tgttgagaat atttttttga tgggaaggca cttatttttg atcgttcgct
    12661 caaagaagca tcgaacgctg ggttttagat aaaaaaactg agagaaaaca ggatgcttcc
    12721 atcggattca tcttagataa agtgagatta tatagtgaaa tccgatttga ttttttattg
    12781 cttctggtta tcgattaaat aaaaaagcgc ccatcagggc gcttcgatat acaaattaat
    12841 tcacaaaagc aatattacag gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc
    12901 agtgcaccag aaaccataac tacgacgtca cctttgtgtg ccagaccgct ctgcagagcc
    12961 agttctttac ccagacggta gaaatcatca gtagaagtga tctctttaac cagctgcggc
    13021 acaacgcctt tgctcagtac caactgatga gccgtttttt cgttggtggt cagtgccagt
    13081 atggtggcat ccgggaagta tttacgtact gcgcgagcag atttaccgcc ctgggtagca
    13141 accacgatca gcggagcatc cagtttttca gcagtttcaa cggcaccacg gcataccgct
    13201 tcggtaatgc gcagtttacg gttgtcattg ttgaactcga gacggctgtt catcacgcgg
    13261 tcggtacgtt cgcagatggt cgccatgata gaaaccgctt ccagcgggta tttacctttt
    13321 gcggattcac cagacagcat cactgcgtca gtaccgtcga ggatggcgtt tgcaacgtca
    13381 ccggcttctg cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca
    13441 gtgataacga ctttacgtgc acggatacat ttttcgatca tcatcttctg ggcgaagata
    13501 acttcttcta ccggaatttc tacacccagg tcgccacgcg caaccatgat gccgtcagag
    13561 gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt ggagatgatg
    13621 tggatgtttt cgccgccgtg cgctttcagg tgctcacgga tttcgataac gtcagaacgc
    13681 ttacgaataa aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa gatcaggtcc
    13741 tgtttgtctt tttcagccag tgctggcagg gcaatggaaa cgccaggcag gttcacacct
    13801 ttgttttcgc ccaggtcgcc gttgttcagc actttacaga taactttgtt accttcaatg
    13861 gcagtaactt ccataccgat cagaccatcg tcaaccagta cggtgttgcc aacagacagg
    13921 tcagtagtga aaccttcata cgttaccgca accatttcgc tgttgccgat aacagattta
    13981 tcagtggtga aagtaaaggt ctgacccgct ttcagagaaa cgtcgttacc gccttccagt
    14041 ttcatggtgc ggatttccgg acctttggta tcaagcagga tagcggcggt tttaccagtt
    14101 ttgctcatca cgttgcgcag attctgaatg cgctgaccgt gttctgcata gtcaccatga
    14161 gagaagttca gacgcataac gttcatgcca gcgtccagca ttttagctaa catctcttca
    14221 gattcggttt tcggtccgat ggtgcaaaca attttggtct ttttcatgac agtcttagtc
    14281 tttaagttga gaaggatggg agaaacttgc tttctgggcg cactgccgga aagcgaatcc
    14341 ggtattacga aagtggtggt gccttgctct aaggataggt gacatcgaaa aagcgtgcag
    14401 aggaatgtgt gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc gttgagttct
    14461 acagtccaaa gtgctgaaac cattcaagag tcaattggcg cgcattatac gctgaaatta
    14521 atccaaaagg aaaacaaaaa cagcgtttca aaaaatatac aacttgttag acaataaatt
    14581 gttatcaaag atttaacgtt ccagggaaaa ggttacgcaa aaacttcgct gtttgcgcaa
    14641 cgtctggtgc cgaagttgtc agtgtgagga taagtgaaag ttcatccggt gtataaatat
    14701 atcatcgctc aattgctaca gttaaaataa tttatcgcgt tatttgtctg attttgtttg
    14761 atttttgttt aattggttag attgatatat gctgatagtt ggcatgataa ataggtataa
    14821 agaatgggaa atcggacaaa agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt
    14881 aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa agcatattgt tatcgcaggc
    14941 gtattacgta ctgcattagc caataaacgc attcagcgca gtgagctgga aaagcaggcg
    15001 atggaaaccg tcattaacgc actggtaaaa tgaccattca ggatgaggcg gtaagcttca
    15061 tcctgatatt cttgcttcag ttgcggtcca gttaatcatt ctaaataaca actgctgaaa
    15121 ttatacgaat tgcggtatgt attttaatct ataacgataa tattattttt attttctgca
    15181 atgaacgtca caaataattg tcgcttaagt taaatttatt tcaattgata ataaatatca
    15241 gttcacttat aatttcttgc tttgtaattt taattgataa cgatcaatgt taagggctca
    15301 gaataatcac attatttttt ctcctaacca taaaggattg gtttatcggc gttaaatctt
    15361 ttctcataca ctatcgggtt agggaaaata tctggtgttg gggacagagt cagcagcgta
    15421 ttagttgaat gcttaatatc aggctgctga attgtcagca ctgtgtttgc gaagaggatg
    15481 cgaaataaat gaacccggtt gatcgtccac tattagatat tggcttaacg cgattagaat
    15541 tcttgcgtat atcaggaaaa ggccttgctg gtctaacaat tgcccctgcg ttactgtcac
    15601 ttttgggttg caaacaggaa gatattgata gcggcacagt agggttgata aatacaccca
    15661 aaggggtgct ggtgacccag cgagcgcgat gcacgggttg ccaccgctgt gaaatctctt
    15721 gtaccaactt caacgatggc tcagtaggga cattcttctc ccgtatcaaa atccatcgca
    15781 attatttctt tggcgacaac ggggttggct ctggcggcgg cctgtatggc gatctcaact
    15841 ataccgcgga cacctgccgt caatgcaaag agccgcaatg catgaacgtc tgcccgattg
    15901 gtgcgattac ctggcagcag aaagaaggct gtattaccgt cgatcataaa cgctgtattg
    15961 gctgtagcgc ctgtactacg gcatgcccgt ggatgatggc caccgtaaat accgaaagta
    16021 aaaaatcctc gaaatgtgtg ttatgcggtg aatgcgcaaa cgcctgccca acaggggcgt
    16081 taaaaattat cgagtggaaa gatattactg tgtgaatctt gcaaaggaaa aaatcatggc
    16141 taacggttgg acaggtaata tattaagagt caatctcacg acaggaaata ttaccctcga
    16201 agattccagt aagtttaaaa gttttgtcgg tggcatgggc ttcggctaca aaattatgta
    16261 tgacgaagta ccgccaggca cgaaaccttt cgatgaagcg aataaattag tctttgctac
    16321 cggcccatta actggatctg gtgccccctg tagttctcgc gtaaatatca cctcactttc
    16381 tacttttacc aaaggaaatt tagtcgtcga tgcccatatg ggtggctttt ttgcagcgca
    16441 aatgaaattc gctggatacg acgtcattat tatcgaaggg aaggcgaaat caccggtatg
    16501 gctgaagatt aaagatgaca aagttagcct ggaaaaagcc gatttcttat ggggaaaagg
    16561 gacgcgcgca acgacggaag aaatttgtcg attgaccagt ccggaaacct gtgtggcggc
    16621 tattggtcag gctggggaaa accttgttcc tctctctggc atgttgaata gccgtaacca
    16681 cagcggcggt gcgggaactg gcgcaataat gggttcgaaa aacctgaaag cgattgcggt
    16741 tgaagggacg aaaggggtca acattgccga tcgtcaggag atgaagcgtc tcaacgatta
    16801 catgatgact gaacttattg gtgcgaataa caaccatgtc gtgccaagta cgccgcaatc
    16861 gtgggcagag tattcagatc ccaagtcacg ttggacagca cgtaaagggc tgttttgggg
    16921 cgcggctgaa ggtggtccga ttgaaacggg ggaaattccg ccaggcaatc agaacacggt
    16981 cggctttcgt acctataaat ccgtttttga cttaggtccg gcggcagaga aatacacagt
    17041 aaaaatgagt ggctgccact cttgcccgat ccgttgtatg acccaaatga atattcctcg
    17101 ggtgaaagag tttggcgtgc ccagcacagg tggtaacact tgtgtagcca actttgtcca
    17161 taccaccatc ttcccgaacg gtccgaaaga ttttgaagat aaagacgatg gtcgtgtgat
    17221 tggtaacctg gtgggtctga atctgttcga tgactacggc ctatggtgta actacgggca
    17281 gttgcatcgc gactttacct attgttacag caaaggtgtg ttcaagcgtg ttctgcctgc
    17341 tgaagagtat gcggaaattc gctgggatca actggaagcg ggtgacgtta acttcattaa
    17401 aattaaagat ttttactacc gtctggcgca tcgtgtgggt gagctgagtc acctggctga
    17461 tggttcatat gccatcgcag aacgctggaa tttgggtgaa gagtactggg gctacgcgaa
    17521 aaataaactc tggtcgccgt ttggctatcc ggttcaccat gccaatgaag cgtcagcgca
    17581 ggtcggttcc attgttaact gtatgttcaa ccgtgactgc atgacgcata cccatatcaa
    17641 ctttatt
//
LOCUS       DAJVXP010000083        17492 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVXP010000083 DAJVXP010000000
VERSION     DAJVXP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.083"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..844)
                     /gene="adrA"
                     /locus_tag="OQS10_002654"
                     /gene_synonym="dgcC"
     CDS             complement(<1..844)
                     /gene="adrA"
                     /locus_tag="OQS10_002654"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS2293654.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(946..1266)
                     /gene="psiF"
                     /locus_tag="OQS10_002655"
     CDS             complement(946..1266)
                     /gene="psiF"
                     /locus_tag="OQS10_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS2293655.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1385..2800)
                     /gene="phoA"
                     /locus_tag="OQS10_002656"
     CDS             complement(1385..2800)
                     /gene="phoA"
                     /locus_tag="OQS10_002656"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS2293656.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2901..3161)
                     /gene="iraP"
                     /locus_tag="OQS10_002657"
     CDS             complement(2901..3161)
                     /gene="iraP"
                     /locus_tag="OQS10_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS2293657.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3343..3546)
                     /locus_tag="OQS10_002658"
     CDS             complement(3343..3546)
                     /locus_tag="OQS10_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293658.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3624..4718
                     /gene="ddlA"
                     /locus_tag="OQS10_002659"
     CDS             3624..4718
                     /gene="ddlA"
                     /locus_tag="OQS10_002659"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS2293659.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4742..4954)
                     /locus_tag="OQS10_002660"
     CDS             complement(4742..4954)
                     /locus_tag="OQS10_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS2293660.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5214..5522
                     /locus_tag="OQS10_002661"
     CDS             5214..5522
                     /locus_tag="OQS10_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS2293661.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5581..6675)
                     /gene="yaiW"
                     /locus_tag="OQS10_002662"
     CDS             complement(5581..6675)
                     /gene="yaiW"
                     /locus_tag="OQS10_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS2293662.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6688..7908)
                     /gene="sbmA"
                     /locus_tag="OQS10_002663"
     CDS             complement(6688..7908)
                     /gene="sbmA"
                     /locus_tag="OQS10_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS2293663.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8260..9417
                     /gene="ampH"
                     /locus_tag="OQS10_002664"
     CDS             8260..9417
                     /gene="ampH"
                     /locus_tag="OQS10_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS2293664.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9418..10041)
                     /gene="iprA"
                     /locus_tag="OQS10_002665"
     CDS             complement(9418..10041)
                     /gene="iprA"
                     /locus_tag="OQS10_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS2293665.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10129..13017)
                     /locus_tag="OQS10_002666"
     CDS             complement(10129..13017)
                     /locus_tag="OQS10_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2293666.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13543..14517
                     /gene="hemB"
                     /locus_tag="OQS10_002667"
     CDS             13543..14517
                     /gene="hemB"
                     /locus_tag="OQS10_002667"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS2293667.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14623..15474)
                     /gene="tauD"
                     /locus_tag="OQS10_002668"
     CDS             complement(14623..15474)
                     /gene="tauD"
                     /locus_tag="OQS10_002668"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS2293668.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15471..16298)
                     /gene="tauC"
                     /locus_tag="OQS10_002669"
     CDS             complement(15471..16298)
                     /gene="tauC"
                     /locus_tag="OQS10_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS2293669.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16295..17062)
                     /gene="tauB"
                     /locus_tag="OQS10_002670"
     CDS             complement(16295..17062)
                     /gene="tauB"
                     /locus_tag="OQS10_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS2293670.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17075..>17492)
                     /gene="tauA"
                     /locus_tag="OQS10_002671"
     CDS             complement(17075..>17492)
                     /gene="tauA"
                     /locus_tag="OQS10_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="with TauB and TauC is responsible for taurine
                     uptake; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2293671.1"
                     /translation="LEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAK
                     SAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTG
                     PVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4371 a         4543 c         4218 g         4360 t
ORIGIN      
        1 taccgcgcag ggtaatttgt aactgtcggg taagcgccac aatcgcttca tcgcccacat
       61 catgacccca ggtatcgttg atgctcttga aatggtcgat atcgataatc agtaacgttg
      121 catcgcgatt atgccgccga cagttatcaa attcattgcg taacatagtt tcccaatgac
      181 gtcggttata cacgcccgtc atgccgtcgc gagtactcat gacctgcaac ctgcgtttat
      241 gttccgccag tttggttgcc gtctggtagc tgacccagcc aaacagcaga ggataaacga
      301 caataatggg aagggagagc caccattcca gcggcgcact attgaacgac acggtaatgc
      361 ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa caccagaccc gcgacaaaca
      421 gacgggggcc gcctgccccc atcaaattca gacacataat catcaacata gcggtggaag
      481 gcagcacgtt tacgcccatt acgcctaccc acattcccgc taatactgca tcggttttta
      541 agttgtaaat ttcccggcta agcggatcga cggccctgct cgctatctgc caggctaaat
      601 gcggccagac gaacgcccag ccgaccaaca ccagccacca ccagcccggc ggcgggtgcg
      661 aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag gccaaccgca cggggtagtc
      721 tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc gttttgagga gttaaaggag
      781 gttcctcccc atgcgccgcc gcttttttga aaaagttttc atcattcatt atttttggga
      841 acattcagaa acaattttcc caaattatag agacggggct aaaggcagaa gtatgaaatt
      901 tcgggtgagc cactttcgta gctcacccgt tagcactatc aggtatcagg cggctttctt
      961 gaggcaggca ctcataaact tattacgatc atcacctttc agagattgtt gcgttgcttg
     1021 attattacat tcgcgcatct tttgctgctg tggcgtcaaa cttttttcac caggcgcaga
     1081 cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt tcaacgcctg
     1141 cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgtggggtta aggttctctc
     1201 ggcagcgccg acggtggtta aaaaaaccag accaaaaagc aaggtaacca gtaatgttat
     1261 tttcatagca ccatccctct tcatgtttta accatgagcg tttgcgcccg tgatctgcca
     1321 ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac tgccgggcgc
     1381 ggttttattt cagccccaga gcggctttca tggtgtagaa gagatcggtc tggtcggtca
     1441 gtccaacaac attggcggca tgcggaccat acgccgcaat acgcaactga ctgccggtat
     1501 gttcttgtga atcctcttcg gaattcccgt aactcatcac catcactgcg ccatctttag
     1561 tatttagcgc ctgagtgagg cccggcgctt tggtatccgg cgcaacaacc tggctggcgt
     1621 gggcgtgatc agcggtgact atgaccagcg tgttaccctc ctttttagcg aattccagcg
     1681 cccgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa ggattcgcag
     1741 cgtgatcctg tttatcgatt gacgcacctt caacttgcag gaaaaatcct ttctcatttt
     1801 tactcaacaa ttcaatggtt ttgtcggtca tctgtgccag ggttggtaca ctgtcattac
     1861 gttgcggatt aggcgtacag gtgactgcgg gcttatcgat attgccgtgg tacgttgctt
     1921 tcggccctag ccagcgcact ggcatattgc cgtcagcaaa caggccaagc aggggttttt
     1981 gctgattcgc ttccgtcacc gaattcagtg aggcagcatc gttcaccaac tgataaccac
     2041 gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc accagcggtt gccgtttcag
     2101 caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg agcgttaagc agctgttcgg
     2161 taatcgatcc ttttccgcct ttttccagag cgttacccgg acatttttca ctggtcgcgc
     2221 tcggaccgta gcatttgcgc gaagtcacat gtgccaccag cgcagcgggc gtggcatcct
     2281 gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc ggcttttgcc atttccagaa
     2341 tcgttgggtg atctttttcg tgaatatcga cgcccagcgc gccgttatag gttttgacac
     2401 cggttgacca ggcggttgct gatgcagccg agtcggtgac gtagtccggt ttgccagttt
     2461 ttttattcag cgcatagtga gtgtattgcc cggtaagcgg taaggcatcg atacctttaa
     2521 aaaagccgcc cgcaccttcg gcataattac gtgcggcagt aatttccgaa tcccccatcc
     2581 catcgccaat cagcaaaata atattttttg caggtttatc gctaagagaa tcacgcagag
     2641 cggcggtctg atcactcgtt aagcggcgag caccgccggg tgcagtaata tcgccctgag
     2701 cagcccggtt ttccagaaca ggcatttctg gtgtccgggc ttttgtcaca ggggtaaaca
     2761 gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac tttattttct ccatgtacaa
     2821 atacattaaa aaataaaaac aaagcgacta taagtctcgg ccgtgacaac tttatgacag
     2881 ctgttgaaaa gattaacttt ttactgacga ggatgcttca ataacttctt tacgtaatcg
     2941 cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg gctttacctc gtacagcgcc
     3001 ccctctacct gatcaatcaa ccgctgttgg tcattttgcg ccatattgcg aagcattgca
     3061 gtgacgataa tctccaaagc ttctacctgc gcacacagtt ctttcgactc ttcttctttt
     3121 tgggcaagct taaataacaa ctcagcaatg agatttttca tgtctgtatt tccttatcca
     3181 aagtatggag aagttaacat tatgtttcac aacttcatag tgtctggaag tattattttt
     3241 atgacaaagg aaatactttg cgcaaatatt ttttgtttga ttaccagcga aacgtttctc
     3301 ttatttaaat aattacccac acaaaatata ggcttttaat tatcaacctt tattataaat
     3361 aaaagttatg aaatatattc tcttcgttaa cttataatta agagaaaaaa actcaccgca
     3421 ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga tctcgcgcac
     3481 atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
     3541 caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
     3601 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
     3661 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
     3721 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
     3781 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
     3841 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
     3901 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
     3961 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
     4021 tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc
     4081 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
     4141 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
     4201 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
     4261 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
     4321 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
     4381 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     4441 ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     4501 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     4561 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     4621 aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg gcgctgaagc
     4681 gtcacgctgc ggataacgca ttgaaagcca caatgtaata ttttaacgcc cgataatatt
     4741 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaacccc gccagccaga
     4801 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     4861 ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     4921 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     4981 tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta attgttaata
     5041 aatttaactt aaggttgcca ttttgtaact cacaacgaag attcacgctt cgggaatatt
     5101 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     5161 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     5221 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     5281 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     5341 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     5401 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     5461 taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     5521 aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat tcctgtttga
     5581 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     5641 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     5701 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc
     5761 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     5821 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     5881 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg cgttctgaaa
     5941 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     6001 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     6061 actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     6121 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc
     6181 aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     6241 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     6301 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     6361 ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc
     6421 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     6481 ttcaaaggtg atggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     6541 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     6601 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     6661 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     6721 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     6781 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     6841 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     6901 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     6961 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     7021 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     7081 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcca
     7141 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     7201 gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca ggaaggcgat
     7261 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     7321 catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt
     7381 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acatagtgac tgacaaagaa
     7441 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     7501 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     7561 atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa accaggtgac
     7621 gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     7681 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     7741 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     7801 cgccacccag tctcccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     7861 ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     7921 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccacta caaagcgtca
     7981 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     8041 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     8101 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     8161 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     8221 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     8281 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     8341 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     8401 gcaacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc
     8461 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     8521 ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag
     8581 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg
     8641 acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     8701 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac
     8761 ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     8821 ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     8881 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     8941 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     9001 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     9061 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     9121 tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     9181 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     9241 acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     9301 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     9361 aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     9421 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     9481 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     9541 atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga
     9601 taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca cgtcgcgtaa
     9661 ttccagaatt ctattttgcc aagctaacca gtaaaaagcg tctcgccaga gttgattttg
     9721 ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     9781 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     9841 ggcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa ctccctctaa
     9901 aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct tttcattatt
     9961 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
    10021 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
    10081 gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt accaggtata
    10141 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
    10201 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
    10261 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
    10321 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt cgctgtaggt
    10381 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
    10441 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaacgct
    10501 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaagcc
    10561 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
    10621 agacaggtcg ttgttgaagt gagagtagct caagctacca tcaacaaaga cgttgttcgc
    10681 gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatctacct gaccagaacg
    10741 gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact tagcgttgtt
    10801 accgtcaact ttggtatcaa caccgaccat gatgccgtta acatcctgat catagttgat
    10861 ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc cgccgttatc
    10921 agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt ccaggttcca
    10981 gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg tcagctccat
    11041 ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg cacccaggtc
    11101 agctttgtta gcagcagaga aggtcgcgtt ggttttggta tcatcgatat agatgagctg
    11161 cttacctgcg taatcagcaa tcgcaccagc gccagtggag ttgtcgatac gaactttgta
    11221 gttaccggtt gcagctacaa cgttgtctgc agagctgttg tcaacttcgt cgccattgtt
    11281 aatgtcgttg ttaccattaa cggtcaagtg accatcagag ttcatcgcaa taacaccata
    11341 accgtagtta gctttgctgg tgtcgttggt acgatcgttg acgaggtctg cattcaggat
    11401 gtaatcatta ctatgaatgt caaacacacc tgcgtaaaca ttaccgttgt tatctttaac
    11461 gttgccactg gtcagttcca gagtatcagt tgcaaatacg ctggagatgt cattcatgtt
    11521 ggtgtttaca tccagcacac caccgttagt aacggtaatg gtattggcat acagcggtgc
    11581 atatgcccaa gctgaagcag tatcttcacc gaagttaacg gttgagaagg tatcaatggt
    11641 caggtgatcg gtgttaactt caccgttaac ttccattgaa gaaccaccgt tcagagtgat
    11701 ggtgtcagaa gtcagagaag aatctgcact cacgttaacc tgagaaccgc tgttgatcgc
    11761 cagggtatcg atcagggaag ttttcgtggt ttcccaatca gaaccgttgt tcagaacaac
    11821 atcgaatacg ccgctctgga atgcgttagc gttatcaaca cgattccagc catcttcaga
    11881 gtaagaagcg tacgggttca ggctgtttgg tgcaacgtcg tagaaggctt cgtcagcaac
    11941 aacaacgtca taatcaatat tcgctgaacc aacccatttg gtgttgttca gagtcaggtt
    12001 caggtgatcg acgttctgat cgtcagttgc ccaaccgcca tgagtgtaag cagtggtaga
    12061 gttgtcaatc aggtgacccg tcgcgtcaaa gttagcgttc caggtactgt caaatacaac
    12121 gtcaccaagc aactgagagt tgttgaacgt aacgttgttg accatacgat aatcagaagt
    12181 gtcatcatcg atgaaggcta acgcaacatc atcagcagga gatgcgccat tgccgtagtc
    12241 acttggttca acagaattgc cgaaatgacc acgatctgat tgttcgtctt cagtccatga
    12301 accagaagtt acagtagagt cattaacaac gatgttgttg tcccactgat gaccttcggt
    12361 attgtaggtc gcattaccga ggtcgtgttg aaccaaagta atacctgcaa catgggagtt
    12421 gttgctgatg ttgatgttac tttcaacatc cagagtcaca gcggtaccaa gaccagcggt
    12481 taccaaacga tcataatctg tcttagatgt tttaccgtct gcatccagat aagtatcggt
    12541 gaagtagaag gcgtcaaaat catcgtcaat agtagaatta gcgatgttca gagtgaagat
    12601 gtcgccatca acccagtcgt ggccagtata atcgctaccg ttggtccaga cgtagtcgcc
    12661 ataaccaacc tggtaagatg taaccatacc atgaacaatt gagttggaga tgttcagtgt
    12721 attaccggta tctgtgccat aatcgtggtt cagataataa gtagaaatta caccatttac
    12781 agtgtcgccg ctgaaagtgg tatcaaagta accatcagct gcgtggtcat atacataacc
    12841 accgtaagtc agataatcgt gatcaatttt agtgctgtca ccccaattgt agatggtggc
    12901 tgcgtcatcg tgatacccga aggtgttata agtagctgca ttagcctgag aggtgatagc
    12961 cagagtgcaa gccaatgcta attgtgatac tacaagtttc tttttccagg agtgcatttg
    13021 tcatccctcc tcagggacgt aataagttga tccatcaatt atcaatgcat agaaaacata
    13081 ttatgtttcg cgctaacgat tatgctgcgt tacagacgaa tggttcaatt attctttccc
    13141 ctaagtccga atgtggacat atataattta tggagaaaaa cacgcatcaa attgataaaa
    13201 ggacgatgtt tcaacatgtt gcagataatg ttatttttga tacatacgcg ttataaatat
    13261 caaaaacaca aaccagataa atatcttatc aatatacaac aaagtttgaa ttattcaatt
    13321 tatagcagca caaccaaacg ccaaaaaaaa taataaccaa gagctataaa taaagattaa
    13381 aaatattttc agtgaatatg aaattattgc tatttctatt acaaagatgt cctggtctct
    13441 ctttcattct cttcttgtca acatcacgac aactttcgta aaacactcct gccccgcttc
    13501 aggtatactg tgcaccttaa ttccacaaac atcaggcaga ccatgacaga cttaatccaa
    13561 cgccctcgtc gcctacgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt
    13621 agccttaacg acctggtgtt gccgatcttt gttgaagaag aaattgacga ctacaaagcc
    13681 gttgaagcca tgccaggcgt gatgcgcatt ccagagaaac atctggcacg cgaaattgaa
    13741 cgcatcgcca acgccggtat ccgttccgta atgacttttg gcatctctca ccataccgat
    13801 gaaaccggca gcgacgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc
    13861 aagcagactg tgccagaaat gatcgtcatg tcagacactt gtttctgtga atacacttct
    13921 cacggtcact gcggtgtgct gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat
    13981 ttaggcaagc aagccgtggt tgcagctgct gcaggcgcag acttcatcgc cccttccgcc
    14041 gcgatggacg gccaggtaca ggcgattcgt caggcgctgg acgctgcagg ctttaaagat
    14101 acggcgatta tgtcgtattc gaccaagttc gcctcttcct tttatggccc gttccgtgaa
    14161 gctgccggaa gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt
    14221 cgcgaggcga ttcgtgaatc actgctggat gaagcccagg gcgcagactg cctgatggtt
    14281 aaacctgctg gagcgtacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg
    14341 attggcgcgt atcaggtgag cggtgagtac gcgatgatta agttcgccgc gctggcgggt
    14401 gctatagatg aagagaaagt cgtgctcgaa agcttaggtt caattaagcg tgcgggtgcg
    14461 gatctgattt tcagctactt tgcgctggat ttggctgaga agaagattct gcgttaattt
    14521 ttttcctctc agaaaggggg agttaacctg ttgaaagtac ggtgaacgcc ccctcaccct
    14581 aaccctctcc ccaagggggc gagggaaccg tccgcactcg tatcaccccg cccgataaaa
    14641 cggtttatcc ccaaggatcg tcgcccgatg cataatccgc cgctgtggca ggtaatcggc
    14701 attggcatag tgctgagtca cgcggttatc ccaaatcgca atatcattcg gttgccagcg
    14761 ccaacgcacc tgaaactccg gtttagtgat atgggcgaat aaaaagccta acaaggcttc
    14821 gctctctttc tcgctgacat cgacaattcg cgtagtaaag ccttcattca caaacagcgc
    14881 ctgtttaccg ctcaccggat gcgttcgcac caccggatgt agcaacggag gatttttcgc
    14941 gaccgcctcg cgccagcgtt gatgttcctc ctcggttttg cggtatttgt attccgggaa
    15001 cgatttacgg aaatcatgct ccgcacgcag cccgctcagc agctggcgga agggaacaga
    15061 gagcgcctca taggccgcaa taccgctggt ccagagcgta tcaccgccgg tcgaaggtaa
    15121 ctctttagct gccagaatcg cccctgcggg tggcgtttca ataaatgtca catcggtatg
    15181 ccagttgtcg ttatccggcg gattatcgtt atgggtatcc agcacgatga tctcgtcaac
    15241 cccttcggca tgagggtaaa cagggtgaat atgcagttcg ccaaaacgct gggccagcgc
    15301 gcgttgctgc tgcggcgtaa tagcttgatc gcgcagaaat accacctgat agcgcagcac
    15361 cgcatggtaa agctgttcaa actgattatc gcttaacggg cgcgtcaggt cggcacccga
    15421 aatttgtgcg ccgatatacg gccccagcgg ggtaatactc agacgttcac tcattgtact
    15481 tctccatgcc agggcgtcag acggcgctgt aacgcgcgca gacccagttc taaaagaaag
    15541 gcgataatcg caatcaccgc gatccccgcc agtaccacgt cagtcgcgag aaattcaccc
    15601 gctgactgaa ccataaatcc taaaccgcgt gtcgcggcaa tcagctccgc cgctaccagc
    15661 gtagaccagc ccacacccag accaatgcgt aatccggtga ggatttccgg cagcgcgccg
    15721 ggcaaaatga caaaccacag cacctgcgca cggctggcac ccagcgactg ggcggcacga
    15781 atgcgaacct gctgcgcgct tttcaccccc gccagcaccg acatcgccac cggtgcaaaa
    15841 atcgctaaat agatcaataa gatcttcgag gtttcaccaa taccaaacca gatcaccatc
    15901 agcggcaaat aagccagcgg cggcaccgga cgataaagtt cgattaccgg atccagaatg
    15961 ccgcgcaccg tcgggcttag ccccatggcg atcccgaccg gaataccgat caccaccgcc
    16021 gcgagtagcg ccagcacaat gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg
    16081 tccataaatc cctgcggtcc ggcaatggtg agcagttttg ccagtacttg ttgcggcggc
    16141 ggcaaaaaca gcgggctaat cagttgcagc gccgccaccg tccaccatac agtgagtaaa
    16201 accgctaacg tgccaatgct taaggtcacc tgacgcgaga gcggccagcg ccatttcagc
    16261 cgccgcgaat gcagtttttc attaatgagc acactcatga gaacgcctcc cgttgctcaa
    16321 atacgcggct taaaacatat tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc
    16381 ggctcgactc tcccgcaaca aagcggcgag caaagttgag cggcagccgc tccagcacac
    16441 ggccagggcc ggatgaaagc agaaccagtt cagtcgccat aaacaccgct tcttctatat
    16501 cgtgggtaat caacagcacc tgcttgcccg tctcctgcca gagtttcagc agcagagttt
    16561 gcatctggtc gcgggtgaag gcgtccagcg caccaaacgg ttcgtcgagt aataacagct
    16621 ggggattcgc cgccagcgcg cgggcaatcc ccacccgctg acgttgacca ccggaaagct
    16681 gccagatata gcgtttttct gcgccttcca gccccacttt tttcagcatc tggtgcgcga
    16741 tttccagtcg ctgcattttc tctatacctg ccaattgcag gccgaacgcc acgttgtcct
    16801 gtacattgcg ccacggtagt agcccttcat tctgaaaaac caccccgcgc tctgctcccg
    16861 gtccctcaat acgcttaccc gccagttgaa tgctgccatg ctgataaggc acaaaaccgg
    16921 caatcagatt cagcagggtg gttttaccgc agccggacgg ccccagcacc accagtagct
    16981 cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg tttgccgcca taatcggcat
    17041 agagatgaga gatttgcagc atctgcgcct ccttttattg cacgaagcgc gaggtaacgt
    17101 actggctgta atcattcgct acagccggga ccttgccctg ctctttcaaa aactgcgcgg
    17161 tgtcgatgat tgctttgttc accggtccgg tcagttctgc cgtttgttgc tgcggcgtca
    17221 gataggtatt ccccttcacc agccccggaa cgtcaccttc cggcacgccg cttaaacgcg
    17281 ccagtttgct gatgttttcc ggctgtttca gccacgcgtc tgggttagca atgtacggtt
    17341 gctgagcatc gatggcgctt ttagcgaacg ctttcacgac ctcaggatgt ttctcggcaa
    17401 aatctttgcg caccacccag acgtccagcg ttggcgcacc ccactgcccg acctgttcag
    17461 aatcggtcaa caccttgccg tctttttcca gg
//
LOCUS       DAJVXP010000084        17471 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVXP010000084 DAJVXP010000000
VERSION     DAJVXP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.084"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..932)
                     /gene="cysB"
                     /locus_tag="OQS10_002672"
     CDS             complement(<1..932)
                     /gene="cysB"
                     /locus_tag="OQS10_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS2293672.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNE"
     gene            complement(1142..3739)
                     /gene="topA"
                     /locus_tag="OQS10_002673"
     CDS             complement(1142..3739)
                     /gene="topA"
                     /locus_tag="OQS10_002673"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS2293673.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4119..4370
                     /locus_tag="OQS10_002674"
     CDS             4119..4370
                     /locus_tag="OQS10_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS2293674.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4406..5455)
                     /gene="sohB"
                     /locus_tag="OQS10_002675"
     CDS             complement(4406..5455)
                     /gene="sohB"
                     /locus_tag="OQS10_002675"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS2293675.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5675..6433
                     /locus_tag="OQS10_002676"
     CDS             5675..6433
                     /locus_tag="OQS10_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS2293676.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6430..7020
                     /gene="cobO"
                     /locus_tag="OQS10_002677"
     CDS             6430..7020
                     /gene="cobO"
                     /locus_tag="OQS10_002677"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS2293677.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7060..7932)
                     /gene="rluB"
                     /locus_tag="OQS10_002678"
     CDS             complement(7060..7932)
                     /gene="rluB"
                     /locus_tag="OQS10_002678"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS2293678.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8145..9259)
                     /locus_tag="OQS10_002679"
                     /pseudo
     CDS             complement(8145..9259)
                     /locus_tag="OQS10_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9287..9907)
                     /locus_tag="OQS10_002680"
     CDS             complement(9287..9907)
                     /locus_tag="OQS10_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS2293679.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9904..10785)
                     /gene="yciV"
                     /locus_tag="OQS10_002681"
     CDS             complement(9904..10785)
                     /gene="yciV"
                     /locus_tag="OQS10_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS2293680.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10923..10967
                     /gene="trpL"
                     /locus_tag="OQS10_002682"
     CDS             10923..10967
                     /gene="trpL"
                     /locus_tag="OQS10_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS2293681.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10944..11037
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11059..12621
                     /gene="trpE"
                     /locus_tag="OQS10_002683"
     CDS             11059..12621
                     /gene="trpE"
                     /locus_tag="OQS10_002683"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS2293682.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12621..14216
                     /gene="trpD"
                     /locus_tag="OQS10_002684"
     CDS             12621..14216
                     /gene="trpD"
                     /locus_tag="OQS10_002684"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS2293683.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14217..15578
                     /gene="trpCF"
                     /locus_tag="OQS10_002685"
     CDS             14217..15578
                     /gene="trpCF"
                     /locus_tag="OQS10_002685"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS2293684.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15590..16783
                     /gene="trpB"
                     /locus_tag="OQS10_002686"
     CDS             15590..16783
                     /gene="trpB"
                     /locus_tag="OQS10_002686"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS2293685.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16783..>17471
                     /gene="trpA"
                     /locus_tag="OQS10_002687"
     CDS             16783..>17471
                     /gene="trpA"
                     /locus_tag="OQS10_002687"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS2293686.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAG"
BASE COUNT         4158 a         4655 c         4397 g         4261 t
ORIGIN      
        1 tcttcattag agcgcaatgc gacagccgca tcaacgacat cacgcgttaa atgcggtgca
       61 aaacgctgaa tgaaatcata catataactg cgcaagaaag tactacggcg aaaaccaatt
      121 ttggttgtac tgtggctgaa gatatcgtga gcatcaacac gcacaaggtc gggatcggcg
      181 accggatcca ccgccatgct ggcaatgacc cctaccccca gccctaaccg gacgtaagtt
      241 ttaatgacgt cagcatccgt tgccgtgaag acgatacgcg gcgttaaccc tgcgcggtta
      301 aaggcagtat ccagttctga acgtccggta aagccgaagg tatatgtcac caacggatat
      361 tgcgccagtt cttcaatggt aatggctttt ttgcctgcca gcgggtgatc cggagtgact
      421 acaatagccc gattccagtg gtagcacggt aacatcacta aatcttcata cagatgcagc
      481 gcttctgtgg cgatagtgaa atcagcattg cctttagaga cggcatcagc aatttgtgtc
      541 ggcgagccct ggtgcatatg caaagaaacg cggggataac gctcaataaa gcctttgata
      601 acgtttggta acgcgtagcg tgcctgggta tgcgtggtgg cgatatacag tgagccttta
      661 tccggccagg tgtgctctcc ggcaaccgat tttatggcat cgactttcga caggacttcg
      721 cgagcgatac gaattatttc ttgccctgct ggcgttacct gcgtcaggtg cttgccgctt
      781 cgggaaaaaa tttgaatgcc tagctcgtct tccagcattc tgacttgttt actgatcccg
      841 ggttgtgatg tgtaaagtcc ttccgctgtt gatgagacat tcaggttatg attgaccacc
      901 tcaacaatat agcgaagttg ttgtaatttc atgttaaacc atccacttag acgccatcag
      961 aacatcgttt tttcgtcaca cctggcgggt gactaatacg atttaataat aaaaaaggtg
     1021 ctaactataa ccactatatc atttatagcc ttagtgtata ggtgtgaaga aaaaataata
     1081 tgcaataaaa aagggccgct ttcgcgaccc tttgtttata aaaacctgac agaattaaag
     1141 gttatttttt tccttcaacc catttgccat caacataaaa tgctgaccag ccagtcgcct
     1201 ttccgtcttt ttccgaagag acatattgct gtttggtttt acggctaaag cgaaccatgg
     1261 tcttattacc ttccggatcc tgctgtggcg catcggccag ataacgcagt ttttccggca
     1321 gacggtcgcg gaagcgataa agctcttcca ccagtggcgc acgcgtttca cgcgatttcg
     1381 ggaaagtgtt ggcagccagg aaaacacccg cagcgccgtc acgcagcacg aaataagcat
     1441 ctgatttttc gcacggcagc tcaggtaatg gcaccggatc ttctttcggt ggtgccactt
     1501 cgccgttacg taaaatctta cgtgtgtttt tacactcttc gttggtgcag gccatgtatt
     1561 taccgaaacg ccccattttc aggtgcattt cagagccaca tttttcacac tcaacgatcg
     1621 ggccgtcata acctttaatg cggaattcgc cctcttcgat ctcgtaaccg tcgcaggttg
     1681 ggttattacc acagacatgc aacttacgtt tcggatcgat gagatagctg tccatcgccg
     1741 tgccgcattt cgggcaacga cgttttgcgc gcagcgcgtt ggtttcagcg tcttcgcctt
     1801 ccagtacgtt cagcacttcg ttttccggca ccaggttaat ggtggttttg caacgctctt
     1861 tcggcggcag cgcataacca gaacagccaa ggaatacccc ggtgctcgct gtgcgaatcc
     1921 ccattttgcg accacaagtc gggcagtcaa tgctggtcag aaccatctgg ttcgggcgca
     1981 taccaccctc ttccggatct ttttcagctt tatctaactg ctgggtgaaa tccgagaaga
     2041 agtgatccag tacagctttc cactctgctt cgtgatttgc caccaggtcg aggctgtttt
     2101 ccatctgtgc ggtgaagtcg tagttcatta actcgcggaa attttcttca aggcgatcgg
     2161 tgacgatttc gccaattttt tccgcataga aacgacgatt ttctactcgc acgtagccac
     2221 gatcctgaat ggtcgaaatg atcgacgcat aggtagacgg acgaccgata ccgcgttttt
     2281 ccagctcttt aaccagcgat gcttcactga aacgggctgg cggcttggta aagtgctggg
     2341 ctggtgtaag ttcaacgagc gtcagagcat cgcctttatt aactgctggt aagatgcgat
     2401 cttcatcgcc tttacgcaac gcaggcatca cttttgtcca gccatcaaaa cgcaaaatac
     2461 gaccgcgtgc tttcaggcgg aaatcgcccg caccaatggt cagcgtcgtg gagtcatatt
     2521 tcgctggggt catctggcag gctacgaact gacgccagat taactggtac agtttctgcg
     2581 catctgcttc catatccttc agcgattccg ccatcacatt gacgtcagaa ggacgaatcg
     2641 cttcgtgcgc ttcctgtgag ttttctttgc tggcgtactg attcggactt tccggcagat
     2701 atttcttacc aaaattatcg ctgatataac cacgaaccat atttaccgcg tcctgactca
     2761 ggttagtgga gtcggtacgc atgtaagtga tatagcctgc ttcatacaaa cgctgcgcca
     2821 tcatcatggt ttttttcacg ccaaatccaa gacgggtgct ggcagcttgt tgcagcgtag
     2881 aggtaataaa aggagcgcca ggtttactgg ttgtcggttt gtcttcacgt tccagcacgc
     2941 tgtagcgcgc tttttccagc agacttaccg cagcctgagt ttgttctttg ttgaccggac
     3001 ggaacggttt gtcgttctga tgagtcacct gtaacgccaa cgcttcacca gatggcgtgg
     3061 tcgtgctggc atcgacttcc cagaactctt ccggcacgaa cgctttaatt tcacgctcac
     3121 gctcgaccac caggcgaacc gccaccgact gcacacgacc ggcagacaga ccacgagcga
     3181 tctttttcca tagcagcggc gaaaccatat accccaccac gcggtccata aagcgacgcg
     3241 cctgctgggc attaacacga tcaatattca gctcacccgg tttgttaaat gcctggcgga
     3301 tcgcgttttt agtaatttcg ttaaacacca ctcggctata gcgcgcatca tcacccccaa
     3361 tcacttcccg caggtgccat gcaatggctt ccccttcgcg gtcaaggtcg gttgcgagat
     3421 agatgtggtc ggctttttca gccagttgtt tcagttcaga gacgaccttc tctttaccag
     3481 gcaacacttc atagtgcgcc tcccaattgt gccacgggtc aacccccata cggttgacga
     3541 gagcgccacg ttcatccttt ttaggctttt tagccgtctt ggtggaggta gagtcggcac
     3601 tctttttggc agctgagcca ctggtcggca aatcgcggat gtgaccgacg ctggatttca
     3661 ccacgtagtc actacccaga tacttgttga tcgttttggc ttttgccggg gactcaacga
     3721 tgacaagagc tttacccata ttcaccttac ctaatttgat tcttccagga atgcgtcgca
     3781 cgttgataaa ccttccactg gcaacgagtt accgatatgg actcgttctc tgcggatatc
     3841 aaccccttgt cgtgactctg ttcacgaaaa ttcaggtgac tgagttaaca ggtcttggcc
     3901 tacaggcgct atagcacgac ggaattttgg tcgaatgtca agcaaatcta ttgccagatt
     3961 gacgaaagcg tcacactgta cctgataaaa tccgatacgc aactttatta gcatgcaaaa
     4021 tccaaagttg ccagtaaggc gctcttgcca ccagcgtggt atatttgtct gcaagccatg
     4081 cctggcgtag actaatgtcc ttgtttaagg agtaaataat gaacaaagag acacaaccca
     4141 tcgaccgcga aacgctgttg aaagaggcca acaaaatcat tcgtgagcat gaggatacgc
     4201 tcgcagggat tgaagccacc ggcgtcacgc agcgtaatgg cgtgctggta tttatcggcg
     4261 actacttttt agatgaacaa ggattgccaa cagccaaaag caccgcggtg tttaatatgt
     4321 tcaaacacct ggcccatgtg ctttcagaaa aatatcatct ggttgattaa agcaaaacgc
     4381 gaggtcttag cctcgcgttt gtcttttaca tcaatggctt ttgaccccgc tgccaccagc
     4441 gtagcaacaa tcgatcggcg ctctctgccg cgctgccggt gaatcggtca atgagtcgtt
     4501 tacgctgcat atagcgtaca ttaaccactt cacggccttc catcaggcta agaataactt
     4561 catcactggt gttgatttca tcaaccaggc ctttctctac cgcctgttgt ccgtaccagt
     4621 gttcacccgt tgccacctgt tcaatatcca gagacggacg catacgcttc acaaaatctt
     4681 taaataactg atgcgtttcg ttcagctctt cgcggaattt ctcccgccct tcttcggtat
     4741 tttcacccag caaggtcagc gtgcgcttat actgcccggc ggtgtgcagt tcgatatcaa
     4801 tatctttgct tttcaggaag cggttaaagt tgggcatttg cgccaccacc ccaatggaac
     4861 ccacaatagc aaacggtgcg gaaacaattt tgtccgccac acaggccatc atgtaaccgc
     4921 cgctggcagc gactttgtct accgtaacag ttaaaggaat gtttttatca cgcagacgct
     4981 gcagctgcga agccgccaac ccgtaaccat gcaccatgcc accagggctt tccagacgga
     5041 gcacaacctg atcctgcggt ttgaatgctg cgagtaccgc cgttatctct tcacgtagcg
     5101 agttcacttc atgggcgtcc atgctgcctt taaaatccag cacccagacg cgaggtttac
     5161 tgtcagttgc cacctcgccc agtttggctt tcgcttttgc tgctttcgct tcttgcttgt
     5221 gctttttctt ctgcgctttg tgccactgtt tttgctgatg tgagtccatc agcgccgcgg
     5281 ccagttcttc tttcatctcc ttatactgtt cgctgagatt gttgacccgt aactcgccac
     5341 gctggcgttt attacgttga gcaacattga caataatggc ggcaatcgcc gcaatcgcta
     5401 gcacaacggt aacgattttc gccaaaaaca aaccatattc agacaacaat tccacgagtc
     5461 cccaccttgg ttaaacaacg ctttggctca cagtttacaa caggcatgtt aagccgtctc
     5521 gcagagatgt tattgcagcg aaagtatgac aaagtatcca ttttgcggtg ttatcgcccc
     5581 aggctgattg aaatgccctt ctgtttcagg cataaagccc caaagtcata aagtacactg
     5641 gcagcgcgac agacaacgtc gttgaggagt agccatgcat taccagccaa aacaagattt
     5701 actcaatgat cgcattatcc tggtgacggg agccagcgat gggattggtc gtgaagccgc
     5761 gatgacgtat gcacgctatg gtgcgacagt gattctgttg ggccgtaatg aagaaaaatt
     5821 acgtcaggta gccagccaca taaacgaaga aactgggcgt cagccacagt ggtttattct
     5881 cgatttgctg acctgcacgt ccgaaaattg ccaacaactg gcacagcgca ttgtcgttaa
     5941 ttatccgcgt ctggatggtg ttttgcataa tgccggattg ctcggcgatg tttgcccaat
     6001 gagcgaacaa aatccgcagg tctggcagga cgtcatgcag gtcaacgtta atgccacctt
     6061 tatgctcacc caggcactgc ttcctttatt actcaaatcg gacgccggtt cactggtctt
     6121 tacttcatca agcgttgggc gtcagggacg agccaactgg ggtgcatatg cagcgtcgaa
     6181 atttgccacc gaagggatga tgcaggtact ggccgatgaa tatcagcagc gcctgcgcgt
     6241 caactgcatt aacccaggcg gtacgcgcac cgcaatgcgc gcaagcgcct tcccgaccga
     6301 agatccacag aaacttaaaa cacccgctga tatcatgccg ctctacctct ggctgatggg
     6361 cgatgacagc cgccgtaaaa ccggcatgac ctttgacgcc caaccgggcc gtaaaccagg
     6421 aatttcccaa tgagtgatga acgctaccaa cagcgtcagc agcgagtgaa agaaaaagta
     6481 gatgctcgtg tggcccaggc ccaggatgaa cgcggtatta tcatcgtctt taccggcaat
     6541 ggaaaaggca aaaccaccgc ggcatttggt acggcaacac gcgcagttgg tcacggaaaa
     6601 aaagtgggcg tcgtgcagtt tattaaaggc acctggccta atggcgaacg caatctgctg
     6661 gagccacatg gcgttgagtt tcaggtgatg gcaacgggct ttacctggga tacacaaaac
     6721 cgcgagtctg ataccgccgc ctgccgcgaa gtctggcaac atgcaaagcg gatgcttgct
     6781 gattcctcac tggatatggt tttgcttgat gaactgacgt atatggtggc gtatgactat
     6841 ttgccactgg aagaagtggt gcaggcgtta aatgaacgtc cacatcaaca gacggtgatt
     6901 atcacgggtc gaggctgtca tcgggatatt cttgaactgg cggatacggt aagtgagctg
     6961 cgccccatca aacatgcgtt tgatgccggt gttaaggcgc aaatagggat tgattattaa
     7021 tctgtaaaaa tgccggatat gacgtccggc atactttgat taaccgttat tacgcccgcc
     7081 agaacgacga ccgccgctca cctgactgtg acgtttcacc gcacgacgaa tctgattagc
     7141 cttcatgcga cgacggtctt tttctacagc gactttagag ctggtttctg gcggaagctc
     7201 caccagttcg cgcagatagt tagtctgggc gaggtccagc tccgtccagc caccgcgtgg
     7261 cagacctttt ggcagtggga tatcaccgta acgaacacgg atcaggcggc tcacctgcac
     7321 accaaccgct tcccacagac gacgaacttc acggttacgg ccttcggtca gagtcacgtt
     7381 gtaccactgg ttgatccctt cgccgccgct gaacttgatg gttttaaaag ctgccggacc
     7441 atcttccagt tgcacgccac gactcaaatc acgcagtttc gcgtcgtcaa cctgaccaaa
     7501 tacacgcacg gcgtattcac gttcaacttc acggcttgga tgcattaaac ggtttgccag
     7561 ttcaccatcg gtggtgaaca gcagcagacc acaggtgtta acgtccagac gacctacggc
     7621 aatccagcgt gcgccacgca gctttggcag acggtcaaac accgtcggac gcccttccgg
     7681 atcgttacgg gtacacaact caccttccgg tttgtaatag gccagaacgc gacaaatttg
     7741 ttcagcggac tcacgtaccg aaatcagatg accatcgata cgaattttca gcccaggggt
     7801 aacttcaacg cgatcgccga gtttagcaat tttgccatca acactcacac gaccggcttc
     7861 aataatagat tcgatttcac ggcgggagcc gtggccagcg cgggccagca ctttctgtaa
     7921 tttttcgctc atagagcttc cttttggtgt cgccttccca ggcgtctgat atgcttttgt
     7981 gcaacagtga gtcagaactt acatctgact cactgatagt aaagtctgtt tgaactcttc
     8041 aggccttaag taaacgactc gacgttttaa ctggcctggc tttgaggcgc aacatactac
     8101 actatcttta tggttaaagc ttgtttcgat taacatcagc ttaattacca gccaccaccg
     8161 cctccgccac ccgcgccgcc accagcacca ccaccggaat aacttcgccc caacaaactg
     8221 gttttggccc ccagactcag gtgcatctgt aatgaaaaca aaacgccact acgggcaatt
     8281 tccggggcgc taacagccgc tgtgagtgat ggggtgatac gtgctaccca ttctcttcca
     8341 aggtttgctg agactgccca acctagcaac gcgtaatcca gtttccgtgt ttcccctttc
     8401 cgccgacgcc cactgtgtgt tgctgcctct ttacgcgcaa gatagttaac gatagcttca
     8461 ccgtgggcat aacgttgctg cccagtttgt gtaaactttg gcagaaaaat ataccccatg
     8521 ccagtgagat aaccggtcag gaaaacagcg ctaaaataac ccgctggcat ataccagtaa
     8581 aagaatacat ctccgacatt gatgtagaga gaatagaagg ccacgcctga acaaatcagt
     8641 ggtaaacaat tcttccgtcc tcaaggatgc ggccatttcg atatgtgtcg ccctcttctc
     8701 cagtaaagag atcgatttcg ctaaatggat tacccgcgat atcagggaac ttgagtgaaa
     8761 atcgggtctt atagatttca aacggccagt ggttaccagt cacgttccag attaacagat
     8821 ctgaatctcc ctcacgcagg aaagcatttt taacctgata atgaatttga taatgatagt
     8881 cgccttgagg caagaaacgt tgtttatcgc ccacgacaat actcattaac ccgatatgcc
     8941 agtcatgatg ccagggttca ggaataccat cacgggtgac gccaacaata tgatagttca
     9001 gcagtgcagc gtccccacca ggctgcatcc agagtcgtgg taaatcacga taaaatccgt
     9061 gtttaatctg ctgatttcgc gcctgaaaat gaatattttc tttgatgtcg agcaccccat
     9121 ccggttgcag agtggcttca acatcaaaga gcgaaatccc ttcttcataa gaagggatct
     9181 ccgtgttttc tgcataactt agagatgata caaatagccc tgctatcaac agcaaaatgc
     9241 agcgaaactt ccctgccata gtcttccatc cgtggaacgc aatttgttat aagaaaggct
     9301 tcacatcacc tacgccttca cgcaccacga ccggcgtatc atcggtaaga tcaataaccg
     9361 ttgtcggttt ctgcccgaga tagccgccgt gaataatcaa atctacctgt ttttccagac
     9421 ggtctttaat ttcttcggga tcggattcgg taaattcgct gcctggcagc attagcgaag
     9481 tggaaagcat cggttcgccc agtgcttcaa gcaacgcctg cgcaatcggg ttagaaggca
     9541 cacgcatacc gatggttttg cgtttttcct gcaacagacg gcgtggcact tccttcgtcc
     9601 ccttcaggat gaaggtatag ttgcccggcg tgttgttttt cattaaacga aacgcaacgt
     9661 tatcaacaaa tgaataggtc gagagttcag aaagatcgcg acacatcagg gtaaagttgt
     9721 gaccgtccgg cagctggcga atacgacaaa tacgctccat cgcgttttta tcttcaattt
     9781 tacagccgag cgcatagccg gaatcagttg gataaacaat caccccgcct ttacgcacga
     9841 tctccaccgc ctggttgatc agacgttgct gtgggttatc aggatgaata taaaaaaact
     9901 ggctcataat tccctctctg tggtgttctg cggctgttcc catagctgcc agacgccttc
     9961 aacgcctgcg ggcagccaga gtttacgacc tagttcaatc cacgggcatg gctgatgaaa
    10021 atcagatcct tgtgatgccc ataaatgatg ctgacgcgca agggccgcca gctgggtacg
    10081 ttcattgggc gattgctggc actgcgcgac ttccatcgcg tcaccgtggt gttcggcaaa
    10141 atgcgcaacc aatcttttca gccatttagc actaagattg taccgcccag gatgagctag
    10201 caccgcctta ccgccagaat gatgaatgac atcaatagct tgttctattg tacaccactg
    10261 tggcggaacg tatccggttt tcccgcgcgc cagatatttt ttaaagacat ccgccattga
    10321 actggctttg ccgcacccca caaggaaacg cgcaaaatga ccgcgcgtca ccgcaccgcc
    10381 ctgcgccagt cgtcgcgcgc cttccagtgc ccctggtatt tgcgcttttt caagtctttc
    10441 ggcaatcagt tgcgcccgct gattgcgccg ttctgtctgc tgcgcgagga actcacacat
    10501 tagcggatga gtaatatcaa tattcagccc gacaatatga atttcatgat tttcccagac
    10561 cgtggaaatt tccacgccgg gaataagatt cagcgccagt cccgaacgtg aaatttcctc
    10621 tcttgctggc acgattgcag ctgtgttgtc atggtcggtg atcgccaggg taccgacgcg
    10681 catctcgact gcacggtgca ccaatacttc tggcgtcagg cagccatcgg aagctgtggt
    10741 atggctgtgc aggtcgtaaa tcactgcgta attcgtgtcg ctcaaggcgc actcccgttc
    10801 tggataatgt tttttgcgcc gacatcataa cagttctggc aaatattctg aaatcagctg
    10861 ttgacaatta atcatcgaac tagttaacta gtgcgcaagt tcacgtaaaa agggtatcga
    10921 caatgaaagc aattttcgta ctgaaagctt ggtggcgcac ttcctgaaac gggcagtgta
    10981 ttcaccatgc gtaaagcaat cagataccca gcccgcctaa tgagcgggct tttttttgaa
    11041 caaaattaga gaataacaat gcaaacacaa aaaccgactc tcgaactgct aacctgcgaa
    11101 ggcgcttatc gcgacaaccc gactgcgctt tttcaccagt tgtgtgggaa tcgtccggca
    11161 acgctgctgc tggaatccgc agatatcgac agcaaagatg atttaaaaag cctgctactg
    11221 gtagacagtg cgctgcgcat tacagcatta ggtgacactg tcacaattca ggcactttcc
    11281 ggcaacggcg aagccctgct ggcactactg gataacgccc tgcctgcggg tgtggaaaat
    11341 gaacaatcac caaactgccg tgtgctgcgc ttcccccctg tcagtccact gctggatgaa
    11401 gacgcccgct tatgctccct ttcggttttt gacgctttcc gtttattgca gaatctgttg
    11461 aatgtaccga aggaagaacg agaagcaatg ttcttcggcg gcctgttctc ttatgacctt
    11521 gtggcgggat ttgaagattt accgcaactg tcagcggaaa ataactgccc tgatttctgt
    11581 ttttatctcg ctgaaacgct gatggtgatt gaccatcaga aaaaaaacac ccgcattcag
    11641 gccagcctgt ttgctccgaa tgaagaagaa aaacaacgtc tcactgctcg cctgaacgat
    11701 cttcgccagc agctgaccga agccgcgccg ccgctgccgg tggtttccgt gccgcatatg
    11761 cgttgtgaat gtaatcagag cgatgaagag ttcggtggcg tagtgcgttt gttgcaaaaa
    11821 gcgattcgcg ctggagaaat tttccaggtg gtgccatctc gccgtttctc tctgccctgc
    11881 ccgtcaccgc tggcggccta ttacgtgctg aaaaagagta atcccagccc gtacatgttt
    11941 tttatgcagg ataatgattt caccctgttt ggcgcgtcgc cggaaagttc gctcaagtat
    12001 gacgccacca gccgccagat tgagatctac ccgattgccg gaacacgtcc acgcggtcgt
    12061 cgtgccgatg gctcgctgga cagagacctc gacagccgca tcgaactgga aatgcgtacc
    12121 gatcataaag agctttctga acatctgatg ctggtggatc tcgcccgtaa tgatctggca
    12181 cgcatttgca cccccggcag cagctacgtc gccgatctta ccaaagttga ccgttactct
    12241 tacgtgatgc acctggtctc ccgcgtggtc ggtgagctgc gccacgatct cgacgccctg
    12301 cacgcttacc gcgcctgtat gaatatgggg acgttaagcg gtgcgccgaa agtacgcgcc
    12361 atgcagttaa ttgccgaggc ggaaggtcgt cgccgcggca gctacggcgg cgcggtaggt
    12421 tatttcaccg cgcatggcga tctcgacacc tgcattgtga tccgctcggc gctggtggaa
    12481 aacggtatcg ccaccgtgca agccggtgct ggcgtagtcc ttgattctat tccgcagtcg
    12541 gaagccgacg aaacccgtaa taaagcccgc gctgtactgc gcgctattgc caccgcgcat
    12601 catgcacagg agactttctg atggctgaca ttctgctgct cgataatatc gactctttta
    12661 cgtacaacct ggcagatcag ttgcgcagca atgggcataa tgtggtgatt taccgcaacc
    12721 atattccggc gcagacctta attgaacgcc tggcgacgat gagcaatccg atactgatgc
    12781 tttctcctgg ccccggtgtg ccgagcgaag ccggttgtat gccggaactc ctcacccgct
    12841 tgcgtggcaa gctgcccatt attggcattt gcctcggaca tcaggcaatt gtcgaagctt
    12901 acgggggcta tgtcggtcag gcgggcgaaa ttcttcacgg taaagcgtcg agcattgaac
    12961 atgacggtca ggcgatgttt gccggattaa caaacccgct gccggtggcg cgttatcact
    13021 cgctggttgg cagtaacatt ccggccggtt taaccatcaa cgcccatttt aatggcatgg
    13081 tgatggcagt acgtcacgat gcggatcgcg tttgtggatt ccagttccat ccggaatcca
    13141 ttctcaccac ccagggcgct cgcctgctgg aacaaacgct ggcctgggcg cagcagaaac
    13201 tagagccagc caacacgctg caaccgattc tggaaaaact gtatcaggcg cagacgctta
    13261 gccaacaaga aagccaccag ctgttttcag cggtggtgcg tggcgagctg aagccggaac
    13321 aactggcggc ggcgctggtg agcatgaaaa ttcgcggtga gcacccgaac gagatcgccg
    13381 gggcagcaac cgcgctactg gaaaacgcag cgccgttccc gcgcccagat tatctgtttg
    13441 ctgatatcgt cggtactggc ggtgacggca gcaacagtat caatatttct accgccagtg
    13501 cgtttgtcgc cgctgcctgt gggctgaaag tggcgaaaca cggcaaccgt agcgtctcca
    13561 gtaaatctgg ttcgtccgat ctgctggcgg cgttcggtat taatcttgat atgaacgccg
    13621 ataaatcgcg ccaggcgctg gatgagttag gtgtttgttt cctctttgcg ccgaagtatc
    13681 acaccggatt ccgccacgcg atgccggttc gccagcaact gaaaacccgc accctgttca
    13741 atgtgctggg gccattgatt aacccggcgc atccgccgct ggcgttaatt ggtgtttata
    13801 gtccggaact ggtgctgccg attgccgaaa ccttgcgcgt gctggggtat caacgcgcgg
    13861 cggtggtgca cagcggcggg atggatgaag tttcattaca cgcgccgaca atcgttgccg
    13921 agctgcatga cggcgaaatt aagagctatc aattgaccgc tgaagatttt ggcctgactc
    13981 cctaccacca ggagcaactg gcaggcggaa caccggaaga aaaccgtgac attttaacac
    14041 gcttgttaca aggtaaaggc gacgccgccc atgaagcagc cgtcgctgcg aacgtcgcca
    14101 tgttaatgcg cctgcatggc catgaagatc tgcaagccaa tgcgcaaacc gttcttgagg
    14161 tactgcgcag tggttccgct tacgacagag ttaccgcgct ggcggcacga gggtaaatga
    14221 tgcaaaccgt tttagcgaaa atcgtcgcag acaaggcgat ttgggtagaa acccgcaaac
    14281 agcagcaacc gctggccagt tttcagaatg aggttcagcc gagcacgcga catttttatg
    14341 atgcgctaca gggtgcacgc acggcgttta ttctggagtg caagaaagcg tcgccgtcaa
    14401 aaggcgtgat ccgtgatgat ttcgatccgg cacgcattgc ctccatttat aaacattacg
    14461 cttcggcaat ttcggtgctg actgatgaga aatattttca ggggagcttt gatttcctcc
    14521 ccatcgtcag ccaaatcgcc ccgcagccga ttttatgtaa agacttcatt atcgaccctt
    14581 accagatcta tctggcgcgc tattaccagg ccgatgcctg cttattaatg ctttcagtac
    14641 tggatgacga ccaatatcgc cagcttgcag ccgtcgctca cagtctggag atgggggtgc
    14701 tgaccgaagt cagtaatgaa gaggaactgg agcgcgccat tgcattgggg gcaaaggtcg
    14761 ttggcatcaa caaccgcgat ctgcgtgatt tgtcgattga tctcaaccgt acccgcgagc
    14821 ttgcgccgaa actggggcac aacgtgacgg taatcagcga atccggcatc aatacttacg
    14881 ctcaggtgcg cgagttaagc cacttcgcta acggttttct gattggttcg gcgttgatgg
    14941 cccatgacga tttgcacgcc gccgtgcgcc gggtgttgct gggtgagaat aaagtatgtg
    15001 gtctgacgcg tgggcaagat gctaaagcag cttatgacgc gggcgcgatt tacggtgggt
    15061 tgattttggt tgcgacatca ccgcgttgcg tcaacgttga acaggcgcag gaagtgatgg
    15121 ctgcggcacc gttgcagtat gttggcgtgt tccgcaatca cgatattgcc gatgtggtgg
    15181 acaaagctaa ggtgttatcg ctggcggcag tgcaactgca tggtaatgaa gatcagctgt
    15241 atatcgatac gctgcgtgaa gctctgccag cacacgttgc catctggaaa gcattaagcg
    15301 tcggtgaaac cctgcccgcc cgcgagcttc agcacgttga taaatatgtt ttagacaacg
    15361 gccagggtgg aagcgggcaa cgtttcgact ggtcactatt aaatggtcaa tcgcttggca
    15421 acgttctgct ggcggggggc ttaggcgcag ataactgcgt ggaagcggca caaaccggct
    15481 gcgccggact tgattttaat tctgctgtag agtcgcaacc gggcatcaaa gacgcacgtc
    15541 ttttggcctc ggttttccag acgctgcgcg catattaagg aaaggaacaa tgacaacatt
    15601 acttaacccc tattttggtg agtttggcgg catgtacgtg ccacaaatcc tgatgcctgc
    15661 tctgcgccag ctggaagaag cttttgtcag cgcgcaaaaa gatcctgaat ttcaggccca
    15721 gttcaacgac ctactgaaaa actatgccgg gcgtccaacc gcactgacca aatgccagaa
    15781 cattacggct ggcacgaaca ccacgctgta tctgaagcgc gaagatttgc tgcacggcgg
    15841 cgcgcataaa actaaccagg tgctcggtca ggctttactg gcgaagcgga tgggtaaaac
    15901 cgaaatcatc gccgaaaccg gtgccggtca gcatggcgtg gcgtcggccc ttgccagcgc
    15961 cctgctcggc ctgaaatgcc gtatttatat gggtgccaaa gacgttgaac gccagtcgcc
    16021 taacgttttt cgtatgcgct taatgggtgc ggaagtgatc ccggtgcata gcggttccgc
    16081 gacgctgaaa gatgcctgta acgaggcgct gcgcgactgg tccggtagtt acgaaaccgc
    16141 gcactatatg ctgggcaccg cagctggccc gcatccttat ccgaccattg tgcgtgagtt
    16201 tcagcggatg attggcgaag aaaccaaagc gcagattctg gaaagagaag gtcgcctgcc
    16261 ggatgccgtt atcgcctgtg ttggcggcgg ttcgaatgcc atcggcatgt ttgctgattt
    16321 catcaatgaa accaacgtcg gcctgattgg tgtggagcca ggtggtcacg gtatcgaaac
    16381 tggcgagcac ggcgcaccgc taaaacatgg tcgcgtgggt atctatttcg gtatgaaagc
    16441 gccgatgatg caaaccgaag acgggcagat tgaagaatct tactccatct ccgccggatt
    16501 ggatttcccg tctgtcggcc cacaacacgc gtatcttaac agcactggac gcgctgatta
    16561 cgtgtctatt accgatgatg aagcccttga agccttcaag acgctgtgcc tacacgaagg
    16621 gatcatcccg gcgctggaat cctcccacgc cctggcccat gcgctgaaaa tgatgcgcga
    16681 aaacccggaa aaagagcagc tactggtggt taacctttcc ggtcgcggcg ataaagacat
    16741 cttcaccgtt cacgatattt tgaaagcacg aggggaaatc tgatggaacg ctacgaatct
    16801 ctgtttgccc agttgaagga gcgcaaagaa ggcgcattcg ttcctttcgt cacgctcggg
    16861 gatccgggca ttgagcagtc attgaaaatt atcgatacgc taattgaagc cggtgctgac
    16921 gcgctggagt taggcatccc cttctccgac ccactggcag atggcccgac gattcaaaac
    16981 gccactctgc gcgcctttgc ggcaggtgtg actccggcac aatgttttga aatgctggca
    17041 ctgattcgcc agaaacaccc gaccattccc attggcctgt tgatgtatgc caatctggtg
    17101 tttaacaaag gcattgatga gttttatgcc cagtgcgaga aagtcggcgt cgattcggtg
    17161 ctggttgccg atgtgccaat tgaagagtcc gcgcccttcc gccaggccgc gttgcgccat
    17221 aatgtcgcac ctatcttcat ctgcccgcca aatgccgatg acgacctgct gcgccagata
    17281 gcctcttacg gtcgtggtta cacctatttg ctgtcacgag cgggcgtgac cggcgcagaa
    17341 aaccgcgccg cgttacccct caatcatctg gttgcgaagc tgaaagagta caacgctgca
    17401 cctccattgc aggggtttgg tatttccacc ccggatcagg taaaagcagc gattgatgca
    17461 ggagctgcgg g
//
LOCUS       DAJVXP010000085        17448 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVXP010000085 DAJVXP010000000
VERSION     DAJVXP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.085"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..502)
                     /locus_tag="OQS10_002688"
     CDS             complement(<1..502)
                     /locus_tag="OQS10_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS2293687.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OQS10_002689"
     tRNA            complement(688..772)
                     /locus_tag="OQS10_002689"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OQS10_002690"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OQS10_002690"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS2293688.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OQS10_002691"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OQS10_002691"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS2293689.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OQS10_002692"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OQS10_002692"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCS2293690.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OQS10_002693"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OQS10_002693"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCS2293691.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OQS10_002694"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OQS10_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCS2293692.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OQS10_002695"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OQS10_002695"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCS2293693.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OQS10_002696"
     CDS             7117..8619
                     /locus_tag="OQS10_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS2293694.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OQS10_002697"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OQS10_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS2293695.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OQS10_002698"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OQS10_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS2293696.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OQS10_002699"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OQS10_002699"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS2293697.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OQS10_002700"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OQS10_002700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS2293698.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OQS10_002701"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OQS10_002701"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS2293699.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OQS10_002702"
     CDS             complement(16186..17382)
                     /locus_tag="OQS10_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS2293700.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAJVXP010000086        17268 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVXP010000086 DAJVXP010000000
VERSION     DAJVXP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.086"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..302
                     /gene="tnpA"
                     /locus_tag="OQS10_002703"
                     /pseudo
     CDS             1..302
                     /gene="tnpA"
                     /locus_tag="OQS10_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(433..1756)
                     /locus_tag="OQS10_002704"
                     /pseudo
     CDS             complement(433..1756)
                     /locus_tag="OQS10_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1756..3372)
                     /locus_tag="OQS10_002705"
     CDS             complement(1756..3372)
                     /locus_tag="OQS10_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS2293701.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3668..4384
                     /locus_tag="OQS10_002706"
     CDS             3668..4384
                     /locus_tag="OQS10_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS2293702.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4381..6042)
                     /locus_tag="OQS10_002707"
     CDS             complement(4381..6042)
                     /locus_tag="OQS10_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS2293703.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6238..6666)
                     /gene="ibpB"
                     /locus_tag="OQS10_002708"
     CDS             complement(6238..6666)
                     /gene="ibpB"
                     /locus_tag="OQS10_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS2293704.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6778..7191)
                     /gene="ibpA"
                     /locus_tag="OQS10_002709"
     CDS             complement(6778..7191)
                     /gene="ibpA"
                     /locus_tag="OQS10_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS2293705.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7185..7257)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7497..7829
                     /locus_tag="OQS10_002710"
     CDS             7497..7829
                     /locus_tag="OQS10_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS2293706.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7831..9045)
                     /locus_tag="OQS10_002711"
     CDS             complement(7831..9045)
                     /locus_tag="OQS10_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS2293707.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9146..10210
                     /gene="cbrA"
                     /locus_tag="OQS10_002712"
     CDS             9146..10210
                     /gene="cbrA"
                     /locus_tag="OQS10_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS2293708.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10207..11544)
                     /locus_tag="OQS10_002713"
     CDS             complement(10207..11544)
                     /locus_tag="OQS10_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2293709.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11619..12767)
                     /gene="dgoD"
                     /locus_tag="OQS10_002714"
                     /pseudo
     CDS             complement(11619..12767)
                     /gene="dgoD"
                     /locus_tag="OQS10_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12764..13381)
                     /gene="dgoA"
                     /locus_tag="OQS10_002715"
     CDS             complement(12764..13381)
                     /gene="dgoA"
                     /locus_tag="OQS10_002715"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS2293710.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13365..14243)
                     /gene="dgoK"
                     /locus_tag="OQS10_002716"
     CDS             complement(13365..14243)
                     /gene="dgoK"
                     /locus_tag="OQS10_002716"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS2293711.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14240..14929)
                     /gene="dgoR"
                     /locus_tag="OQS10_002717"
     CDS             complement(14240..14929)
                     /gene="dgoR"
                     /locus_tag="OQS10_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS2293712.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14898..15530
                     /locus_tag="OQS10_002718"
                     /pseudo
     CDS             14898..15530
                     /locus_tag="OQS10_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15576..16388)
                     /gene="yidA"
                     /locus_tag="OQS10_002719"
     CDS             complement(15576..16388)
                     /gene="yidA"
                     /locus_tag="OQS10_002719"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS2293713.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16503..16901)
                     /locus_tag="OQS10_002720"
     CDS             complement(16503..16901)
                     /locus_tag="OQS10_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS2293714.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17141..>17268)
                     /locus_tag="OQS10_002721"
     CDS             complement(17141..>17268)
                     /locus_tag="OQS10_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HCS2293715.1"
                     /translation="RVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAANIDI"
BASE COUNT         4331 a         4614 c         4327 g         3996 t
ORIGIN      
        1 aactacgcac ttacttttca aatgtatgtg ctgattttga agctgaactg gtttaaatgg
       61 atggcgaacc agatcacgtc catttgttaa ttaactatcc tcccaaactg gcgatatcca
      121 gtctggtaaa cagcctcaaa ggcgtatcgg gtaggttact gcgacgagat cgaccagata
      181 ttgcagtcag gtattactac aaaggcgttt tgtggagtcc tggctatttt gccaatagct
      241 gcggaggtgc gccaatatcc gtcatccgcc aatacattga acaacagcaa acacctggtt
      301 aggtggaaaa ccgcgcctta tatccccgcc ctgaaggacg gggttttacg gcgcaccgga
      361 taaatttggt tatgcgccgt cagccgtgct aaagaatacg gaccagcacc caacggatgc
      421 cagtccacac aattaacgta actctggcca gtacgctttg ttggcctcca gcaactcatc
      481 cagaatagct ttggcgaccg aggcgctcgg taccgttttc gataacgtaa tcgcctgcca
      541 caggtgctga tatgaacgct gttcccaggc gtccacgacc agtttttcca ccgccacttg
      601 ctgactcatt aaccctttct gaaactgcgg gatatcgccg accaccagtg gttctggtcc
      661 gttgtgcccg accagacacg ggatctcgac catcgcttca tcatcaaagt tatggattgc
      721 cccgttgtta ggcacaatca gcaacatccg ctcctgagtg ttgaaggcaa ttgccgccgc
      781 cagatcgacg atgtatgacg catgttcgtc aatctccagc ttggccgccg ctgaatttcc
      841 tgccgctgta atggcgcggc aagcatcgaa aacctgtttc tcgcgatgtt ccattcacct
      901 cattcgcgcg ggtatgttca gggttggaat gctgaacaac ataatccggg aagagataat
      961 atttcagata ggtgttcggc aatgtatccg ggtccagtgc ctggacatcc cgcgcttttg
     1021 cataggtgtc gttccagctc gcttcagtat gtgtatcttg ctgcggtgga acataaccgt
     1081 gcttcgagac atattgccgc agctggggca tcaggtcgtt gccctgcaaa tcttcaattg
     1141 atgtccacca gccaaagtgg ttcaggccgt agtagcgcac gcgcatctgt ttgcgatctt
     1201 gcagcccaac aatttgcgcc atccggcttt caataccgat tggcatgtca cagatgttga
     1261 ggattttcgc attcgggcgc agacgacgcg tggcttctgc gacaatggct gccgggttgg
     1321 agtagttgag catccaggca tttggtgaat atttttccat ataatccacc agttccagga
     1381 cgccgccaat ggaacgcatg ccgtacgcta ttccgccggg tccgcaagtt tcctgaccaa
     1441 caacaccgtg gcgcagtggg attttttcat ccagttcgcg catcgggtac ttgccgacgc
     1501 ggatgtgcgc catgacaaaa tcaacgtcgc tgaacgccac ttcaggatcg gtggtgtaac
     1561 taaacgcaat gtccggcgct ttctctttaa ggatgacttt acaggcttcg gcaatcactt
     1621 cctgccgcgc gccatcgtta tcataaaatt tcagtgcacg aagcgggaaa cgctcctgat
     1681 tcgccaggag catcaacacg atgcctggcg taaaggtgct tccaccgcct gcgacaacca
     1741 ctgagaattt ggtcattata ctgcctccgt aatggcaaca ttttctgctg attgatgaga
     1801 attaattaag ctatcgagct gttcacgcag ctgggataca tgcagaccaa tgatcacctg
     1861 aatggcatcg ccactacgga agacgccgtg cgctcccagc tttttaaaga cttcgtcatc
     1921 cagcgtttgt gacatgtcat gcagtgcaat gcgtagacgc gtcgcacaat tgttaatgct
     1981 ggagatattg ccgaccccgc ccagggcttg caggataccg gcagcctgat ccagctcttt
     2041 ttttggctct gccgcggtgg tttggcctcg cgaggctttg tattcggctt ttgagtagag
     2101 tttcacttcc gcatcttcac gtcccggcgt gcacatgttg aactgcagaa tcagggtgcg
     2161 gaaaaccacg aagtacagca gggtaaagca caacccaatg gcgatttggg tcagcatcat
     2221 atccgcgtgg ttgctgaaca tcgggatcca gttttgcggt aaaacctggt caatcagacc
     2281 tccgcccatg ttgcccacca caccaaagag atacatcacg gtcgacattg aggccgcaag
     2341 cacagcgtgt accgcaaaca gcaacggtga aatgaacagg aaggtaaatt ccagcggttc
     2401 ggtaattccc accagcatgg cggttaaggt tgcgggaatc agcaagcccg ccacttttac
     2461 ccgattttcc ggtgcggcag tgaagtacat cgctaaagaa atgcccacgg caccaaagat
     2521 ttttgagtta ccgtgcaggg caaaaccgcc ttccgggaac aacgatttca gcggctcggc
     2581 gctcaaactg aactcttgca gatgctgcgc ccagtacatc tgaatgccgc cttcaacagc
     2641 tgccggaccc aagataaacg gtccgtagat gaagtggtgt aaaccggttg ggatcagaat
     2701 acgttcgagg aaggtgtaaa cccagacccc aagcgcaccc gccgaacgca ggaacgcttg
     2761 cagagattca atccccgttt gtacttttgg ccagccgagc aatgtcagcc aggcacaggg
     2821 gatcatcacc aggaaggcga taatcaccac ataagacgtc ccctggaaaa tgccgagaaa
     2881 aaccggcagt tttttatcga acagacggtt atgcagcgcc gtcacaatgc cggaaatgat
     2941 aattgcgccg ataatgctgg tatcgagggt tttaatcccg gccatcattg tcagaccgct
     3001 acctgccacc gcgtcctgag tgaaatcgac gccgaagtag cttccccagg tcattcccat
     3061 cgcgttgatg aaatagttcc aggtcaggaa actcaccatc accgccagac aagcacgtcc
     3121 ctgcgcttgc ttagcaaggc caatgggtaa accgacagca aaaatcagcg gcatattacg
     3181 gaataccgtc caaccgccct cttcaataat gtgtacgatt tgcgcgaata aactgttcgg
     3241 atcggtcagt gattccccga caaacatcgg gttttgcagc aagatggcaa gacccaccac
     3301 aatcccggcg aagggaaaca gcagcactgg cgtaaacatc gcgccgccaa agcgttgaat
     3361 ttgactgagc atttccacat cctcttttct caattctgta agtaggaagc ctgattggtt
     3421 atgcggcagg cctggagcaa ggataggaag atgcgatcgg cagaacatga cgacccaatg
     3481 ggattcgtga tcgctttcat gcttttcgtt gggcggtaga gatctacgtt ttcgcgaaaa
     3541 agtagacatc tgtgagcaca ggtacacagc atcgaacaca tctttaaaaa aaagatgttt
     3601 tttcaatcga ttaagcagaa cttgtgtgcg cattacccgg gcttgcaggc aaaaaagaga
     3661 tctagagatg atctacaaaa gcattgcgga gcggttaaga attcgactta actccgcaga
     3721 tttcacgcta aacagccttc ttccgggtga aaaaaagctg gcggaagagt ttgcggtatc
     3781 gcggatgacc atccgtaaag ccattgacct gctggtagcg tgggggctgg tggtccgccg
     3841 ccacggtagt ggcacttacc tggtgcgcaa agatgtactg catcaaaccg ccagtctgac
     3901 cgggctggtg gaggtgttaa aacggcaggg aaaaactgtc accagccagg tgctgatttt
     3961 tgaaatcatg cctgcgcctc cggccattgc cagccagtta cggattcaaa tcaacgagca
     4021 gatctacttc tcccgtcgcg ttcgttttgt ggaagggaaa ccgctgatgc tggaagacag
     4081 ctatatgccg gtaaaactgt tccgtaatct ttcgctgcaa catctggaag ggtcgaagtt
     4141 tgaatatatt gaacaagagt gcgggatttt gattggcggt aattatgaaa gcctgacgcc
     4201 ggtgctcgcc gatagactgc tggcgcggca aatgaaggta gcggaacaca cgccactgct
     4261 gcggatcacc tcgttgtcat atagcgagag cggggagttt ttgaattatt cagtgatgtt
     4321 cagaaatgcc agcgaatacc aggtggagta ccatttacgg cgactccacc cggaaaagag
     4381 ttaaccgata ctccagaaga gcaccgccag taattggggg gtgataatgc gcaggaacat
     4441 cactaacgga tagacagtgg cgtaagagag cgccgccgca ccgctagttg gatgaagatt
     4501 attagcaaac gccagcgccg gaggatcggt catggaacct gccagcatcc cgcacatggt
     4561 caggtaattc attttggcta acatccgcgc cagaatgcca acagtaatca gcggaacggc
     4621 ggtgatcagg gcaccataac caatccagct tagcccttcg ccattgacca gggtattcac
     4681 aaaatcccca ccagatttca gaccaacgac cgagaggaac agcacgatcc ccagctcccg
     4741 cagcgcgagg ttggcacttg gcggcataaa ccagtacagc ttgccgatac tgccgatacg
     4801 cccgaggatc aacgccataa tcagcagacc gcccgccagc cccagtttca acgcggccgg
     4861 gaatcccggc acaaagacgg gaatagaacc taacaatacg ccaagcccga tgccaataaa
     4921 cactggcagc atctgaacct gttgcagttt ttgctgcgca ttccccagca cattggcaac
     4981 ggcatcaatt gcggacggac gccccaccag gttgaggata tcgccgaact gcaggctgat
     5041 atcgccactg gcgaccagtt cgaccccggc acggttcagg cgcgagataa caacgtcata
     5101 gcgttcttta aagtgcaggt cgcgaatacg ttttccgagc acgttttcat tggtgaccac
     5161 cacacgctcg acgcgcaaat cagtgtcttt cgtggacagc gaagtatcga cctcctgacc
     5221 aatcaccagt tgcgcattat gtaaatccgc tggctgaccc accagatgca gcaaatcgcc
     5281 cagttggata atggtatctg gcgaaggaac ttttagggtt tcttcgcgtt tcagacgcga
     5341 gcagataatt ttgtcgccgt tgagaatcgg tacatcttta atggctaaat catgcaggtt
     5401 agggttctca acgcgaatat tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga
     5461 ctcgtgctgc tgagcttctg tctcgacatt gacgcggaaa ataacccgca acatccacat
     5521 ggtgaacaaa atcccgcaaa tgccgaatgg atacgccatc gcgtaactca tccccatctg
     5581 atcgaccatt tccattggtg tacccaggtc gcgcaaaatc tgctgccctg cccccagcgc
     5641 tggcgtattg gtaaccgcac cggagaaaat ccccagcact accggcagtg gaatatcaaa
     5701 cagtttatgc aggatggcgg taaccagacc accgatgatg acgatcagaa cagcaaacag
     5761 gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag cccggcccta cctgaatccc
     5821 gatggtataa acgaacagga tcaggccaaa ttcctgaata acatgcagca tatcgctact
     5881 taatgtcatc cccgcctgag aaacaaaatg gccgacgatg atcccaccaa acagcacgcc
     5941 gccaatacct aatcctatgc cgcgaaattt gacgttgccg ataaacaaac cgacgactgc
     6001 caccaaagcc agaatactga ccgttaatgc tatatcactc atcgtccatt tctcttgaga
     6061 taacatttta attgctacgg attatggctg aatcggtgaa gcagatttgc cttatagcgc
     6121 acattattac gggaaagaac gtgccgaaat atcttaaaca gtcgaaattt aacgcattgt
     6181 tgaagaagaa agacaaaaaa agccccacca gaatggcggg gcaaagagaa tagctagtta
     6241 gctatttaac gcgggacgtt cgctgatagc gatacgctgc gctgcgatgg gttcaggctc
     6301 attacgaatt aaatcaatat gcagtaaacc gtttacgaaa gttgcgccag agacttccat
     6361 attttcagcc agcgtaaagc tcaggctaaa tggctgattc ataagccctt gatgcagcca
     6421 ttttttctct tcttttggct gctccggcgt gccttttacg ctcaggcgcg taccttccag
     6481 ttgaatctct aaatcttcct gacggaaacc tgccagcgca agggtaatgc ggtagtggtt
     6541 atcgtcgctt ttctcaatgt tgtacggcgg gaagctctga ctttcaccgg cgttttgcag
     6601 tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc agtggggata aatcgaagtt
     6661 acgcatagtc atttctcctt ctaagaagcg agtaagtacc tgcaaatccg aagattcgca
     6721 tatgctccct gacggcgagc atggagatgt caggccgcgc caggcggcct taaggaatta
     6781 gttgatttcg atacggcgcg gttttttcgc ttccggaatc acgcgttcga gatcgatata
     6841 cagcaaacca tttaccaggt tagcgccacg aacatgaatg ttctcagcta actggaattt
     6901 gcgttcaaag ttgcgttcag cgatgccctg gtacagatag gtgcgctctt tttgttcgtc
     6961 ggcgtgagca cctttcacca ccagcagatt atcctgggcg gtaatttcca gttcgctctc
     7021 agcaaaacca gccacagcga tagcaatgcg gtaatggttt tcgtctacca gttcaacgtt
     7081 atacggaggg tagccgccat tactctggct ctggttgttt tctaagtggt taaacaaacg
     7141 gtcaaatcca atagcagaac ggtaaagcgg ggataaatca aagttacgca taatcaatag
     7201 ctcctgaaat cagcgagaat gtaagacctt ccacaatgga caggtcaggt agccagaaca
     7261 cccatcaggc gcgtcctcat cgactacgat gtaaaaatgg gtctggaaat gaacttttca
     7321 agccttatca gcggactttt ttttgcagtt tatggtctat tgcatagact gagggggcag
     7381 cacgaaacgt taaagtgtga tagccgccac agagcgacgt aatgatgatg ttattttcag
     7441 caagggatcg ctataactca tcaggccaac acccacgatc aacagatgaa tttattatga
     7501 taagaaatgt gttgttagcg ttcatgatat gcagcggaat ggcattactc ggaggatgct
     7561 ccagcgtgat gtcccatacc ggagggaaag aaggaacgta tcccggcacc cgcgccagcg
     7621 ccaccatgat tggcgatgat gaaaccaact ggggcactaa gtcgctggcg attctcgata
     7681 tgccgtttac cgctgtgatg gacacgcttt tattaccgtg ggatgtgttc cgcaaagaca
     7741 gctcggtgcg ctcacgcgtt gaaaaaagcg aagccaacgc gcaagccacc aatgcggtta
     7801 ttccaccggc cagaatgcct gataactgat ttagcgatca gtttcggtga tccacagctg
     7861 gccaccttcc atccacgcca gccattgacc atccggcgag aagaccacgg cgtccgcaga
     7921 aggtggattg gcgtggtttt cggttaaata ctcaacctcg ccgctttgcg catgggcaca
     7981 agcaattcga ttatccagca caaagcccaa ccattctcct gacggatgcc agttaaatgc
     8041 agactgaata tccgttttgt tatgggttaa ctggcgcggc tcgccgccct gtggcgagat
     8101 aagccacagt tgcacaatgc cgttatcatc acgcattaaa aacgcgattt gcgtaccctg
     8161 cggattacag cgcacccagt ggcgcgggac gttgactaac cccggataag cccgatggtg
     8221 ggtaaaggtt aaacgtcgct gcacgacgcc acgcggtggc gcgggcaggg tggtttccgt
     8281 tccacttaac ggcgcatcac ctgccgcttt ccagccagct tcatcttgcg gcaactcaac
     8341 gataaacagc tccggcactt tctcaccctt tggcgaaagt gtgtcgccaa taaacgccag
     8401 cgcgtgattt cctacccatc cttcttcata agcacgattg atttcatcac tgccaggctg
     8461 cggcgtgggc gtggttttac tcaccagcac acaccagtgg ctgccgctgt attcacgcgg
     8521 atgctgcttt tgtacgttga ccggaccaaa cggcgcagca acaccgacgt ttcgcaaatc
     8581 cagcgccgga tcgagttcat gcattacatg gtcgttatag gtaaagctca ccctttcacc
     8641 gttcgggcta aagacatgca catggctgcc gccgcgcagc gctcctggcg tatacggcgc
     8701 ggtaatatcc attgcatcga gattgctcac cttgccgcct tcagcaatga ctccgcgccg
     8761 atgatggaag tcgtaatgcc atgtttcatc aggattttcc gggccgtgaa taaagacata
     8821 tttctctgac ttcggatgaa cggtcaccac gccgacgtgt gcgccctgtg acgcacagta
     8881 gataacctcg acctcaccgg tatggatatt cacacgctca atggtctcgc cggtaaacga
     8941 cgccccagaa ggacgcacgt caaataccag ccactggctg tcgggcgtcc aggtattggt
     9001 attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc ggtcctgata
     9061 caaaacgggt ccagccatca tacttcacaa ggacttcact ttcagacaat agtcttttgc
     9121 catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta ttggcctcgg
     9181 cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga tcgcgctgga
     9241 taaaaagcac cagcatggta ctgaaggttt cagcaaacct tgtggcggtc tgctggcacc
     9301 ggacgcgcag cgatctttta ttcgcgatgg gctgacgctt cctgtcgatg tgatcgctaa
     9361 tccgcagatt ttcagcgtca aaaccgtcga cgtcgccgca tcgctcacgc gtaactacca
     9421 gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat cgctgatccc
     9481 cgccagcgtt gaggtttatc acgacagcct gtgtcgaaaa atctggcgtg aggatgataa
     9541 atggcatgtc atttttcgtg cagacggctg ggagcagcat attaccgccc gctatctggt
     9601 cggtgccgat ggtgccaact cgatggtgcg gcgacatctc tacccggatc atcaaatccg
     9661 taaatatgtc gcaatccagc agtggttcgc agagaaacat ccggtgccgt tctactcctg
     9721 catctttgat aatgcgataa ctgactgtta ttcatggagt atcagcaaag acggttattt
     9781 tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga ctctgaaaga
     9841 gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagcgaaa aatgcacggt
     9901 gctgtttccc tcacgctggc aggattttgt ctgcggtaag gacaacgcct ttctgattgg
     9961 tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg cgctggatag
    10021 cgcagacatt ctgcgttcgg tgttactgaa gcagccagag aagctcaata ccgcttactg
    10081 gcgcgccacc cgcaaactgc gtttaaaact cttcggcaag atagtaaaaa gccgatgcct
    10141 gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcacata ttccacagtt
    10201 gaaggattag ccaacgcgct tcacatcgcc caccagcagg atataagaga gcgcgccaat
    10261 caacgcgacg gcggagatat aaaccagtgc aggtgcgaaa ccgtaaccct gcgccaggta
    10321 ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa acacgccgcc
    10381 ggttaaacca atcaggcgca tcggtgccag agaagaaacc agcgaccagg taattgaagc
    10441 aaaaccgtta ccgaagaatg ccagcgccat caggcacata atcatcatcg gatcgttagt
    10501 gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg gcgttttacg
    10561 cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gctacccagc cagagagcag
    10621 gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt tcagcgccgt
    10681 gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc acagtgtaga
    10741 agccaccgca aattgcccaa gatagacgcc gatcagttta cgatggaaca ccagtttcca
    10801 gtctttggct gttaacggct gacgtgcctc tttcttcact ggcgcatcac catccaccag
    10861 accgccgcca tcacgaatgt aatccagttc agctttgctg atgcctttgg tcaggcgcgg
    10921 cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac cagtcacaat
    10981 gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag tcaaaaacgc
    11041 cagaccgaca aactgaccag acgtataaaa accaacggcg gaggcgcgtt catgttccgg
    11101 gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga aaataccggt
    11161 tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccctgga ataaagtcgc
    11221 cactgaccag ccaaatatcg caataaaata agtcacgcga gaacctacgc gatctaaaaa
    11281 ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa atacatagcc
    11341 catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca cggccaggtt
    11401 agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg tcagataacg
    11461 ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc tccagatttt
    11521 gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat ttttgtattg
    11581 ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc tacgctgtta
    11641 tcttcatgac gccagagtgg attacgccag tccggggcat ttttactgaa ctcaataact
    11701 ttagcttcgt cgatttccac gcctaagccc ggtttcgtta gcggtttaaa gaagccgcca
    11761 accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc tttgttgtaa
    11821 tgaattccca tactttgttc ctgaagtacc gcgttatagg aaacaaagtc gatatgcaga
    11881 caagccgcca gtgcaatcgg tccgagcgga cagtgcggcg caagggtcac gtcataggct
    11941 tctgccattc cggcaatttt gtagcattcg gtaataccgc ctgcatggga gagatccggt
    12001 tgtagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg tgagaacatg
    12061 cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta gtattcggct
    12121 tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag ttctttaatc
    12181 agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa ctcaatctga
    12241 ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac cgcgcgggag
    12301 ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg
    12361 cgcagggttt taataccgtc gatcacatcc gccggacgat cgccgccgac ccaactgtag
    12421 gctttaattt tgtcgcgaac caggccgccc atcagttgcc agaccgacgc attcagcact
    12481 ttacctttga tatcccataa cgcctggtcg atcccggcaa tggcgctcat caggattgga
    12541 ccgccacgat aaaatccggc gcgatacatc acttgccata agtcattgat gcgctatgga
    12601 tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt gcgggcgcgg
    12661 ccttcaatca ctggctcgcc ccagccgacg acgccttcat cggtttcaat tttcaggaac
    12721 atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc actgcctctc
    12781 gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac tgcccggcgc
    12841 gatagagatc gctgcctaag cccgccccag cacaacctgc gtctatccac tgcgccaggt
    12901 tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cgatggcaat accgctttta
    12961 acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc
    13021 ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcag acggtcatgc
    13081 cgtagcccac cgcacggcgg atcacttcac tatggatatt gggcgtaacg atgagctgac
    13141 agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct gcgccaatca
    13201 acgccttgtc gccatacgca tcaacgatgg cgggaatgct ttgctcccat tgtggggaat
    13261 tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca tgcgccagcg
    13321 cctcgtcggg cttaatgccg cgcaaaatgg cgatcagcgg aagtttagtt tgccactgca
    13381 tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc cgtcacgtcg
    13441 caacccatcg cctgaaaggc ttgctggtag cgcgcggtca acgatgttcc ggcgacaagg
    13501 gtgatggcgt gttgatgggc cacatagtcg cgcatactgg cgacctctgc gccaatcaac
    13561 aaaccagaga gaaattcact gacctgttca cggggaagtg ttcccagcac atgcgaggca
    13621 cgaacttcaa aaagctgcgg caatatggcg ggcgtattaa gaccacgctc aaggccagcc
    13681 gtgaaggcat cggcagagtt ttcctgcggc ggcaaacctg caccaatcaa tgagtgattt
    13741 aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat ttgctggcta
    13801 tcggcctgca cccatttgca atgcgtaccg ggcatgacat aaagagagga aggagccaga
    13861 gctcgcgcgc cgatcaattg agtttcttcg ccgcgcatca cattgtggtt atcatcatga
    13921 gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca
    13981 atagacgaaa aatgggcagg aacagataaa tacggagcaa ctttccagcc gacattgctg
    14041 ccgaccattc ctgccattac cactggcgta ttctcttcac gccagtcggt cgtgacttct
    14101 gctaacaccg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg
    14161 ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga tccccagtca
    14221 attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcgatca
    14281 tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca tcgaacagcg
    14341 ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa
    14401 aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc gggttatgca
    14461 ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct
    14521 gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg
    14581 cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact tcgctcatgg
    14641 cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga
    14701 ggtaattcca ctggttacgc ggtgccacaa acgcgccgcg ataacgtttc atttcaatca
    14761 gccgtttcgc catcaacgaa cggaacacct cacggatgat gttgcgcgag gttgcaaact
    14821 cctcacagag ttccgcctca gccggaagcg gcgagcctgg tacgtatttg ccgtgaacga
    14881 tctgtttacc cagcgtaatg acgatgcgat cggttttatt gagagtcatg gagagtcctt
    14941 gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataatcagga aagtatcaat
    15001 cgcagtcaga caccatcacg gggctatcgc aggcaaagga tagtaatgga acaaaaggct
    15061 atgtttttgt aggggaatcg ctggattacc ttatcacaga tggtgccgat gacatcgtta
    15121 agatgctcaa ggatccagct cttaaccggc acaatattca ggttgctgat gacgcaagat
    15181 ttgttttaaa tgcggggaaa aagaaattta ccggcacaat atcactctat tacaactgga
    15241 gcacagaaga agaaaaagca cgagtgacac aatatcgttt tgcttgtggt gttcagcact
    15301 gtacccggac agtagaaaac ctggaaggta ctatccatga gaaaaataaa aacatggatt
    15361 actcaaacgt aatggctttc taccatccat ttaaagtgcg attttatgaa taccattcac
    15421 ccagaggcat tccggatggt gtttccgcag cattactgcc agtgactgtt acgctggaca
    15481 tcattactgc accgctgcaa tttctggttg tatatgcagt aaaccaataa tcagtaagcg
    15541 ggcaaacgcg tttatgctgt ttgcccgccc acagattaat tcagcacata cttctcaata
    15601 gcaaacgcca cgccatcttc aaggttagat ttggtgacaa agttcgccac ttctttcacc
    15661 gaaggaatag cgttatccat cgccacaccg acgcctgcat attcaatcat tgcgatgtcg
    15721 ttttcctgat cgccaatcgc catgatttct tccggtttaa tacctaacac gtcggccagt
    15781 gatttcacac ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca
    15841 cttttcagca cggtatattt ctctttcact tcctgcggaa tacgcgcgat agcctggtcg
    15901 aggatggcgg gttcatcaat catcatcact ttcaggaact gggtattggg gtccattttc
    15961 tccgcttcgc agaacaccag cggaatagtg gcaacgaaag attcatgcac tgtgtagtag
    16021 ctgatatcgc ggttggcggt gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg
    16081 acttcgcgag agagtttttc caggaaacga tagtcgtcat agctgagagc agtttgcgcc
    16141 acggtgctac catcagctgc cttctgtact agcgcgccgt tataagtaat gcagtagtcg
    16201 cccggctgtt ccatatgcag ctctttcagg tagttgtgca cacctgcata cgggcgaccc
    16261 gtcgttagca cgacattcac gccacgggcg cgagctgcgg caatcgcatt tttaacggcg
    16321 ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca tatcgatagc aatgagtttg
    16381 atagccatga gttccccagg tagattggtt cctgacccat gctaacgcga ttccgctcaa
    16441 aaatcagtac aacacccgag ggaaaagggg gatgcaacgc gggttgcatg ctccccattt
    16501 gcttagcgga agagtttccc tttcagcagt tccatacctg cggaaagcag atcgttattg
    16561 gcttgtggtg acacttcacc ttgcggtgag agcgcatcaa taatcttcgg caattgctct
    16621 gccagtaaac tggaagctgt actggtatcc acgccaagtt tttgcccgag atcggacacc
    16681 gcatttgtgc cgagcgccga ttccagttgc tcgccactaa ccgattgatt gccctgttga
    16741 ttactcagcc aggttgagag aatggcccct aagccgccac tttgcagttt ttccagcagc
    16801 acctgaatgc cgccctgctc ctcaacccaa cttaaaatag cctgatattt ccccgcatcg
    16861 cctttcagaa aggcaccgac aacttcatca aaaagcccca tgataatcac ctgtaaagcg
    16921 ttacgtgttg acccaaaaag tatagatttg cgggatgata attgcggatt gcagaaataa
    16981 aaagggcgga gatgatctcc gcccttttct tatagcttct tgccggatgc ggcgtgaacg
    17041 ccttatccgg cctacaaaat catgaaaatt caatacattg caagattttc gtaggcctga
    17101 taagcgtagc gcatcaggca cgctcgcatg gttagcgcca ttaaatatcg atattcgccg
    17161 ctttcagggc gttctcttca ataaacgcac ggcgcggttc aacggcgtcg cccatcagcg
    17221 tggtgaacaa ctggtcggca gcaatcgcat ctttaacggt aacgcgca
//
LOCUS       DAJVXP010000087        17055 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVXP010000087 DAJVXP010000000
VERSION     DAJVXP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.087"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            93..1133
                     /locus_tag="OQS10_002722"
     CDS             93..1133
                     /locus_tag="OQS10_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS2293716.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OQS10_002723"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OQS10_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS2293717.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OQS10_002724"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OQS10_002724"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS2293718.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="OQS10_002725"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="OQS10_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS2293719.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OQS10_002726"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OQS10_002726"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS2293720.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="OQS10_002727"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="OQS10_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS2293721.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9087)
                     /locus_tag="OQS10_002728"
                     /pseudo
     CDS             complement(8440..9087)
                     /locus_tag="OQS10_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OQS10_002729"
     CDS             complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OQS10_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS2293722.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OQS10_002730"
     CDS             complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OQS10_002730"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS2293723.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OQS10_002731"
     CDS             complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OQS10_002731"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS2293724.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12900..14081)
                     /locus_tag="OQS10_002732"
     CDS             complement(12900..14081)
                     /locus_tag="OQS10_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS2293725.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14336..15565
                     /locus_tag="OQS10_002733"
     CDS             14336..15565
                     /locus_tag="OQS10_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS2293726.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16025..16657
                     /locus_tag="OQS10_002734"
     CDS             16025..16657
                     /locus_tag="OQS10_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2293727.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16991..>17055
                     /locus_tag="OQS10_002735"
     CDS             16991..>17055
                     /locus_tag="OQS10_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2293728.1"
                     /translation="MYWIINDNIEFWPEHRKLISV"
BASE COUNT         4518 a         4454 c         3923 g         4160 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgccta ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacatggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggtacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tattccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
     8881 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
     8941 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
     9001 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
     9061 tcctcccagc aaaccaattg ttttcattcc tctctctcct gatgtgtgtt accgcaacaa
     9121 ggctgaataa ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa
     9181 aaaagggccg gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact
     9241 tctcgcctgc catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa
     9301 tgagccagaa agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac
     9361 cggcagcacc aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca
     9421 cccagttcgt ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt
     9481 cagagcacag gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca
     9541 tcgtcatgcc aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca
     9601 tcaggcaata gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag
     9661 ccggtttacg ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg aacataaagg
     9721 tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt
     9781 tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct
     9841 gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc
     9901 aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt
     9961 ccgacgtatc gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg
    10021 ccagccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta
    10081 aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata
    10141 aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc
    10201 gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga
    10261 cagcaatgcc cagcaccacc cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg
    10321 accctataga accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca
    10381 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10441 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10501 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10561 aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg
    10621 tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg
    10681 aacaactcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10741 tgtcgttatg tgatggatat tccaattttc aaattaagtt gaattattga gattattatt
    10801 aaccacctaa ttttacagca gataaaattc ataaagttca ttaattgata attgatatgg
    10861 attatttcat aaccatgata tgaattatga tgacctacag gtataaaaaa ccctgccatg
    10921 aggcagggtc ataaaagtaa gaggaatgaa ttaacgagcc agccaaccgc catctacggc
    10981 aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta tcggccccat
    11041 caggtcactc ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt ccgcgctacg
    11101 ttgttcatct gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc
    11161 attaacatta atgttgtgtt tagcccattc gttcgccatc aatcgcgtca cgcccatcac
    11221 gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11281 cgtgatattg ataatcttgc cgccattgcg ttgcgcgata aagtgtttcg ccgctgcctg
    11341 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11401 gaactcgaga gcatcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11461 accaaactca gctaccgcgc gatccagcag tgctggaatg ccatcaatct ttcgcagatc
    11521 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg
    11581 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11641 ctggcccagt ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc
    11701 acttaaaatc ataacaatac cttattcgtt tatgccgaca actagcgcaa atctttaacg
    11761 gccactatgc cgacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
    11821 cctggttttc accaaccatg ccccagataa aggtataagc tttggttccg acgccggaat
    11881 ggatcgacca gctgggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
    11941 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
    12001 aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag ttacctggag
    12061 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
    12121 tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
    12181 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
    12241 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
    12301 acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc gtaccggcac
    12361 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
    12421 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
    12481 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
    12541 aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
    12601 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
    12661 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
    12721 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
    12781 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
    12841 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
    12901 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
    12961 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
    13021 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
    13081 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
    13141 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
    13201 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
    13261 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
    13321 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
    13381 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
    13441 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
    13501 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
    13561 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
    13621 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
    13681 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
    13741 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
    13801 gctttaagtc cggaaccgca aacgtcatta atcgtgattg cagaaacgct attaggaaga
    13861 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
    13921 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
    13981 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
    14041 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
    14101 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
    14161 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
    14221 gcaaccacta acccaccaat agatgggtag ataaggcgtt aatctcccat acttaacctg
    14281 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
    14341 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
    14401 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
    14461 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
    14521 cctctcatca aaaacatcag catgtgaagg gataacgggc gcggtaacac actactatgg
    14581 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
    14641 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
    14701 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
    14761 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    14821 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
    14881 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    14941 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    15001 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    15061 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    15121 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
    15181 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    15241 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
    15301 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    15361 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
    15421 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
    15481 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
    15541 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
    15601 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
    15661 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
    15721 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
    15781 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
    15841 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atgtcttaat
    15901 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaaga
    15961 tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct
    16021 taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg
    16081 ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt gtttttgtat
    16141 gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct taacaaaaaa
    16201 ccacaatgcg agattaagcg gatatatttt tgtggatttt tcagtaagtt ttttacgtct
    16261 attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga tagttttagt
    16321 cagcgaccgc aatatgcaat cgttagctaa ctactggcga aaacacaaca ccgcaatatc
    16381 tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca gacaactatt
    16441 tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg aattcaccat
    16501 aatggggtat atggtttctg gttataatcc atatcagatt gctgaagtgc tggatatgga
    16561 tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa
    16621 cgaattattt attcgttcac attcggtcca acattgatac tcaacgacca gccagaatca
    16681 tactctgttc atacgggcat ctattgaaat gcccgtattt tataaataag cagtaacccc
    16741 aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat
    16801 atgaatttac aaacaatagt ggcataaatg ttaaccatgt taatttacgt aaagttttac
    16861 gttgcaacat taaagcctca tttcaatcat catgataaat ataaaattaa tatatattta
    16921 tgccgtaaat accaatatac ttagcaaact atgtgatctc catttcgatt gatttagtgt
    16981 ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg agcaccgaaa
    17041 attaatatcg gtaca
//
LOCUS       DAJVXP010000088        16734 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVXP010000088 DAJVXP010000000
VERSION     DAJVXP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.088"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OQS10_002736"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OQS10_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS2293729.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OQS10_002737"
     CDS             2219..2767
                     /locus_tag="OQS10_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS2293730.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OQS10_002738"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OQS10_002738"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS2293731.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OQS10_002739"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OQS10_002739"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS2293732.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OQS10_002740"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OQS10_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS2293733.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQS10_002741"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQS10_002741"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS2293734.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OQS10_002742"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OQS10_002742"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS2293735.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OQS10_002743"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OQS10_002743"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS2293736.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQS10_002744"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQS10_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS2293737.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OQS10_002745"
     CDS             complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OQS10_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS2293738.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OQS10_002746"
                     /pseudo
     CDS             complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OQS10_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OQS10_002747"
                     /pseudo
     CDS             complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OQS10_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            complement(16250..>16734)
                     /gene="ssuE"
                     /locus_tag="OQS10_002748"
     CDS             complement(16250..>16734)
                     /gene="ssuE"
                     /locus_tag="OQS10_002748"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS2293739.1"
                     /translation="VEVYHWNLQNFAPEDLLYARFDSPALKTFTEQLQQADGLIVATP
                     VYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVLSAMKAQEL
                     LHGVFADDSQVIDYHHKPQFTPNLQTRLDTALETFWQALHRRDVQVPDLLSLRGNVHA
                     "
BASE COUNT         4170 a         4359 c         4274 g         3931 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccagatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctcca cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagtga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcga aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggaggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 caatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    13201 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    13501 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    13681 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    13741 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    13861 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    13981 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    14041 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    14101 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    14161 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    14221 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    14281 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    14341 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    14401 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    14461 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    14521 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    14581 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    14641 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    14701 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    14761 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    14821 gctccccgca catgaatatg tttaccgttg aaatcgacgc tttcccccag caataagcgc
    14881 cccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca
    14941 cgccgtcgcc tgccaactct tgtggatcgc tgcctgtgac cagattaaac aacgcacgac
    15001 catttgagag acggtcaagc gtggcggcct ggcgagcggc aacggtaggt gaggttacgc
    15061 tgggacgcag ggcgacaaga aacttcagcc gctgcgtcac cgggatcatc gacgccgcca
    15121 ccagccacgc atcttcacac gatcgccccg ttgggatcag cacaccggta tagcccagac
    15181 gatccgccgc ttgcgcaatt tgttgcagat agccgtgatc aaccgggcgt gaaccttctt
    15241 ccgttccaag ataatgcccg tcaccatggg tcggtaaaaa ccagaacata ttcagactca
    15301 taattgtttt ccttccaggt gagtgggctg ccagatgcgc tggcgaatat caactttttt
    15361 cgacaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc
    15421 agcgctcacc ggtttgatgg tggtgggagg acgatgatcc agatacgagg caatcaccgg
    15481 tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgctcgcgct ggctgcgggt
    15541 taacgcatcg gcctcactaa aggttgccag tacgccctga ataaaagcgc cgtttttttc
    15601 tgcatagggg cgagctgcca gataaaacga tccagtttga ttgagatcgg tgccgtcttt
    15661 cagcacccgc acgccgccct gtaataacgc agcggagtag tagggatccc agatagccca
    15721 ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatca gctggcgtca gataagtggg
    15781 ctggatgtca gtaaacttaa gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga
    15841 actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg ttttgatcgg
    15901 gctgttttct gccaccagaa tcacttcggc tttgggcttc ggtggctcga cgcccacata
    15961 tatcaaatca gccccggcag cctaggcaaa gattggcgga atatccccgg tactgccgag
    16021 atcaatactg ccaacgttta acgcttccaa catttgcgga cccgcgggga actccaccca
    16081 ggagattttt gattgcggat agcgtttttc cagtaactgg tggctttttg ccaataccat
    16141 accaatactg cctttctgat agcctatacg taacgcttca ggcgaggatt ctgcagcaac
    16201 cgcaaaagta gagacgctaa gcaatcccgc cagcgccagt ttaatgatgt tacgcatgga
    16261 cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc
    16321 agaaagtttc tagcgcggta tcaagacggg tttgcagatt tggcgtgaac tggggtttgt
    16381 gatggtaatc aataacttgt gagtcatcgg caaacacgcc gtgcaggagc tcctgggctt
    16441 tcatcgcact taaaactggc ttaagggcat aatcgaccgc cagcagatga gccaccgtac
    16501 cgcccgtcgc cagcggtagc accactttgc cttgcaaagc gcgctctggc agcaggtcga
    16561 gcagggtttt cagcgcgccg gaataggcgg ctttatacac aggtgtagcg actatcagtc
    16621 catcagcctg ttgcagctgt tcggtgaagg tcttgagtgc cggactatcg aaacgagcgt
    16681 aaagcagatc ttccggggcg aagttttgca gattccagtg ataaacctct acat
//
LOCUS       DAJVXP010000089        16555 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVXP010000089 DAJVXP010000000
VERSION     DAJVXP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.089"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OQS10_002749"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OQS10_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS2293740.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OQS10_002750"
     CDS             433..621
                     /locus_tag="OQS10_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS2293741.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OQS10_002751"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OQS10_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCS2293742.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OQS10_002752"
     CDS             816..989
                     /locus_tag="OQS10_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS2293743.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OQS10_002753"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OQS10_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQS10_002754"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OQS10_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS2293744.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OQS10_002755"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OQS10_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS2293745.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQS10_002756"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OQS10_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS2293746.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OQS10_002757"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OQS10_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS2293747.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OQS10_002758"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OQS10_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQS10_002759"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OQS10_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS2293748.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQS10_002760"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OQS10_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS2293749.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="OQS10_002761"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="OQS10_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="OQS10_002762"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="OQS10_002762"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS2293750.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="OQS10_002763"
     CDS             complement(15250..15477)
                     /locus_tag="OQS10_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS2293751.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="OQS10_002764"
     CDS             complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="OQS10_002764"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS2293752.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16155..>16555
                     /locus_tag="OQS10_002765"
     CDS             16155..>16555
                     /locus_tag="OQS10_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2293753.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         4159 a         4071 c         4226 g         4099 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttatcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcact
    16081 agtattcttg ccttaacact catatcattg ttctcctgtt caatgaacca cttagataag
    16141 catagtccga agatatgaag ctgctagtga catacatcag gtggagtact aaagagcaag
    16201 actcaggaga ctccttaaga cggcaaacaa acctcattga tgctttctac tctaagcaca
    16261 aaaacgatta ttaccttctt cctgctcaca gatatgttga taagggtaaa tctggttttc
    16321 accagcaaca taagaatcaa ggttctgact tcagacgtat gtttgaaaat gttatgtctg
    16381 gggctattcc agaaggttcc ttgattgttg tagagaactt tgaccgcttc agtagggctg
    16441 atatcgacac agctatagat gatgtcaggc agatactgag gaagggggtc tctatactga
    16501 ctctgggtga tggtgaactc tacgacaagt cagccttaac agaccctgtg aagtt
//
LOCUS       DAJVXP010000090        16493 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVXP010000090 DAJVXP010000000
VERSION     DAJVXP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.090"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..486)
                     /gene="araH"
                     /locus_tag="OQS10_002766"
     CDS             complement(<1..486)
                     /gene="araH"
                     /locus_tag="OQS10_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS2293754.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SF"
     gene            complement(501..2015)
                     /gene="araG"
                     /locus_tag="OQS10_002767"
     CDS             complement(501..2015)
                     /gene="araG"
                     /locus_tag="OQS10_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS2293755.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2085..3074)
                     /gene="araF"
                     /locus_tag="OQS10_002768"
     CDS             complement(2085..3074)
                     /gene="araF"
                     /locus_tag="OQS10_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCS2293756.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3871..4374
                     /locus_tag="OQS10_002769"
     CDS             3871..4374
                     /locus_tag="OQS10_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS2293757.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4453..4704)
                     /locus_tag="OQS10_002770"
     CDS             complement(4453..4704)
                     /locus_tag="OQS10_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS2293758.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4819..4905)
                     /gene="azuC"
                     /locus_tag="OQS10_002771"
     CDS             complement(4819..4905)
                     /gene="azuC"
                     /locus_tag="OQS10_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS2293759.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5168..5491
                     /locus_tag="OQS10_002772"
     CDS             5168..5491
                     /locus_tag="OQS10_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS2293760.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5662..6159
                     /gene="ftnA"
                     /locus_tag="OQS10_002773"
     CDS             5662..6159
                     /gene="ftnA"
                     /locus_tag="OQS10_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS2293761.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6197..6436)
                     /locus_tag="OQS10_002774"
     CDS             complement(6197..6436)
                     /locus_tag="OQS10_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS2293762.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6627..7838
                     /gene="tyrP"
                     /locus_tag="OQS10_002775"
     CDS             6627..7838
                     /gene="tyrP"
                     /locus_tag="OQS10_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS2293763.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(7900..8565)
                     /gene="yecA"
                     /locus_tag="OQS10_002776"
     CDS             complement(7900..8565)
                     /gene="yecA"
                     /locus_tag="OQS10_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS2293764.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8761..8847)
                     /locus_tag="OQS10_002777"
     tRNA            complement(8761..8847)
                     /locus_tag="OQS10_002777"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8811..8813),aa:Leu,seq:taa)
     gene            complement(8860..8933)
                     /locus_tag="OQS10_002778"
     tRNA            complement(8860..8933)
                     /locus_tag="OQS10_002778"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8899..8901),aa:Cys,seq:gca)
     gene            complement(8988..9063)
                     /locus_tag="OQS10_002779"
     tRNA            complement(8988..9063)
                     /locus_tag="OQS10_002779"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9028..9030),aa:Gly,seq:gcc)
     gene            complement(9215..9763)
                     /gene="pgsA"
                     /locus_tag="OQS10_002780"
     CDS             complement(9215..9763)
                     /gene="pgsA"
                     /locus_tag="OQS10_002780"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS2293765.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9820..11652)
                     /gene="uvrC"
                     /locus_tag="OQS10_002781"
     CDS             complement(9820..11652)
                     /gene="uvrC"
                     /locus_tag="OQS10_002781"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS2293766.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11649..12305)
                     /gene="uvrY"
                     /locus_tag="OQS10_002782"
     CDS             complement(11649..12305)
                     /gene="uvrY"
                     /locus_tag="OQS10_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS2293767.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12601..12777
                     /locus_tag="OQS10_002783"
     CDS             12601..12777
                     /locus_tag="OQS10_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293768.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12764..12988
                     /locus_tag="OQS10_002784"
     CDS             12764..12988
                     /locus_tag="OQS10_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS2293769.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13055..13777)
                     /gene="sdiA"
                     /locus_tag="OQS10_002785"
     CDS             complement(13055..13777)
                     /gene="sdiA"
                     /locus_tag="OQS10_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCS2293770.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(14007..14759)
                     /gene="yecC"
                     /locus_tag="OQS10_002786"
     CDS             complement(14007..14759)
                     /gene="yecC"
                     /locus_tag="OQS10_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS2293771.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(14756..15424)
                     /gene="tcyL"
                     /locus_tag="OQS10_002787"
     CDS             complement(14756..15424)
                     /gene="tcyL"
                     /locus_tag="OQS10_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS2293772.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15439..16425)
                     /gene="dcyD"
                     /locus_tag="OQS10_002788"
     CDS             complement(15439..16425)
                     /gene="dcyD"
                     /locus_tag="OQS10_002788"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS2293773.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         4153 a         4254 c         3817 g         4269 t
ORIGIN      
        1 gaagctttca tcttcgatac cgaccgcttt accgtctgaa atgatgtacg ccagacctcg
       61 aacaatctgc atcgttgcca gtgttgtgat cagagcattt attttcagtt tggcgataac
      121 aaagccattg accaggccac agagaatgcc gagcagcaac cccgctgcca cgccaatcca
      181 caggctttca gtcaggttga taaccaccgc cgtggtgaca cccgcacagg caattacgga
      241 ggcgacagaa aggtcaaagt caccggaagc aaggcagaac aacatgccac acgccaccat
      301 ccccgacatg gaaattgcca ggcccaaccc tttcatatta atgaaggtgg caaaatttgg
      361 gacaaaaatg gcacaggcga taaagagcac cgcaaacacc accagcatgc cgtactgatc
      421 ccagatacgc ccgaagctga atgacgactt aggtgcgcca gaacccgatg tagaaacaga
      481 agacatcata ctctccttac tcaggcaact gcctggctga ctttaggcat cgcaaggctc
      541 agtgcctgac gctcatctgc ctgctcgtgt aacaattcac cggcgatttc accttcccgc
      601 atcaccacaa tccggtcggc aacgccgagg acttcaggta agtcgctgga ggcaaacagc
      661 accgccacac cctgcgccgc cagcgcataa attacgttat atatttcgtg cttagcacca
      721 acatcaatgc tgcgcgtagg ttcatccagc aaaatgacct tcatctcttc cgataaccag
      781 cggcccagaa tggctttttg ctgatttccg cctgagagat tcatgatcag ttgctcagcg
      841 cccggcgttt tgatgttgag cgaacgaatg tgctgatcgg cattgttttc ttcccaaccg
      901 ttgttgatta cacaaccgcc gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg
      961 cgaacggagt gcacgggaat aatgccttcc gctttgcgat cttccgggca gagcatcatg
     1021 cctgcggcaa tggcgtggct cggtttacga atatcgatcg gctgttggtc gatataaacc
     1081 tgaccggcgg tgatttgcgt cccgccaaac aagcctttca ttaattcgct acgccccgcc
     1141 cctaccagac caaacagccc aacaatttca ccactgcgaa ccgccagact tattggcgta
     1201 cgcacgcctg gtgctttcac agcatcaaga cgcaggcgct cctcgccata actacgaggt
     1261 tgccagccgt agatatcgcc aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg
     1321 tcaacctgct gcatatcggt aaaggttttg acataacgtc catctttaaa gacagtgatg
     1381 gcatcgctga gggcaaatat ttcttccata cggtgagaaa cgtataagat cacccgcccc
     1441 tcttttcgca gttcacgaat aacgcggaaa agattgtcga tttcgcgggc agaaagggag
     1501 ctggttggct catcaaaggc gataatttta gcgttacgcg ccagcgcctt ggcgatttca
     1561 accatctgcc actgaccaat ggagagatat ttcagcggcg tgtccgggtc aatatccata
     1621 ccaagatgtt taagttgtaa acccgcctca taattcagca atgagcgatt cacaatgccg
     1681 cctttatgcg gcagctggcc gagatagatg ttttccgcga cggtcatttc cggcacgaga
     1741 tgcagttcct ggtaaataat cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag
     1801 gacatttcct gcccattaat cactacagaa cccgtggttg gcgcatagtt gccgctgagg
     1861 atttttaaga gagttgattt tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg
     1921 gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt tttaccgatg
     1981 ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat cactggtcgt
     2041 tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt accgcctaaa
     2101 ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagcaccac gtcggtaact
     2161 tcggtaaatt ttggcggttc aacgtctttt gctacccagt tgtaaagcat ttcgctggat
     2221 ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt tgcctgtgct
     2281 ttagacagtt cgctcaacgc atccacaccg ttaatgccaa tgccgatgat atcggcagct
     2341 ttaaagccct gaccttccgt cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg
     2401 acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc agcgtcaaat
     2461 gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc cgggaatccg
     2521 gccgctttca gcgcatccat agaacccgtt gtacggcgac gtgccgtatc cagttcgttg
     2581 gcggtaatcg ccatcaccgc gctttctttg acatcccagc cacgtttctg catctcttta
     2641 tacagttcct gaccctgacg ttcgccaatt ttagtcgccg ccatcatcac cagcggaacg
     2701 gtatccattg gcttaccttt ggcgttaaca aactggtcat ccacggcaat gactttcata
     2761 tcgtagccac gcgctttcgc gacgatggca gagccgagtt tggggtccgg agtacaaata
     2821 acgaaacctt ttgcgccact ggcagccagg ctgtcgatcg cgttcaatgt tttttcgcca
     2881 tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt atcggcaaac
     2941 ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc gagcttcagg
     3001 ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc cagggcttta
     3061 gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat
     3121 gactttcgtt aaattaatcg gaaaacaaag cattaccttt tatctaaaag ataagtgacc
     3181 gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat cacatcgcag
     3241 aattacagtg agaacgtgca taaatttagc gggaaaagac ataagggaaa gccaatttgt
     3301 cagacaaatt gtcgaatgca cagcagatta atccataaga ttagcctgga aatccttgtt
     3361 gtctttggta cccatgcggg atgtcttctt tttaaccagt caataggccg cattacctgg
     3421 cgttgagttt ttgaaatggt gtaataaccg caactcaaag atgtggaaaa tgcacgtcat
     3481 tcatttcgcc attaattatc actgtgctca ttaattaaca gaacacgtat aatgagagcc
     3541 atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa ttcaaattca
     3601 ttgattaata tcaacaagat acaaaaagca ctatcattaa aattcattgc agttacattg
     3661 atttcatcaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataccc
     3721 gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg
     3781 accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc
     3841 tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc
     3901 aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg
     3961 tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat
     4021 gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt
     4081 aaagccattg atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg
     4141 atggaagaat acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa
     4201 ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat
     4261 ggtctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc
     4321 cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatca
     4381 tcggcgctaa tgcattgcgc cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa
     4441 gcagcggttt ttttaatggg attcaccctg tggggtaaac ttgagttcaa taagcgcgat
     4501 ggctttttgg attgcccgca tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc
     4561 gatattcttc agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag
     4621 aacatccaga atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg
     4681 atcggcattc agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag
     4741 tagcggcttc acatcttcag cgatagtcac atccaccgtc atcaggacac aaaaaaacct
     4801 gccggagcag gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt
     4861 gcaatagtta ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc
     4921 atttgttata caggtgttat tgtctttgcg cttataatat gacaaccatc acaaaaatca
     4981 atctttatgt gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa
     5041 aaacagcccc cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct
     5101 tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt actcttctgg
     5161 ctggaccatg agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt
     5221 tactcgccaa gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttggttta
     5281 tgaccaggct tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatcgccga
     5341 tcgcgcatgc cagcaggaag gctataccca cgcggtcccc tttggtcagc cagtaggcaa
     5401 ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg
     5461 ccatcattcc gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct
     5521 gattaaagaa atagcggtaa tgcgttttat tcccattcgt atttttaata attgaagttt
     5581 atattttacc ttttgcaaat aataaaataa caaattatag tgacgccaca gcaacaaata
     5641 taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag
     5701 atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat
     5761 cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat
     5821 atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat taataccgtt
     5881 gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac
     5941 gaacaattaa tcacccagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac
     6001 tacccaacat ttaatttcct gcagtggtat gtttctgagc agcatgaaga agagaaactg
     6061 ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt
     6121 atcgacaaag aactctctac cctcgacaca caaaactaat gctctccggc ggcaggttta
     6181 ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa aaaccgtctt
     6241 tcgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc gctgtcatcc
     6301 cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt
     6361 ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac
     6421 cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg
     6481 aagactttgc gccagcgcag gactgaatgc tttttattgt acatttatat ttacactata
     6541 tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag atatcactca
     6601 taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt gtttttatcg
     6661 tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg
     6721 gttttagcgt tacgttaatc ttgttgattg ggctttgggc gttgatgtgc tacacggcgc
     6781 tattactgct ggaggtgtac cagcatgttc cggcagatac cggtctgggc acgctggcaa
     6841 aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt
     6901 atgctctgac tgcggcatac atcagcggtg tcggtgaatt gttggcctcc agcatcagcg
     6961 actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact tttgttgccg
     7021 gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt taaccgtttt ctgttcagcg
     7081 ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt cacaaagtga
     7141 atcttttaac cctgccgttg caacaggggc tggctctgtc agcaatcccg gtgattttta
     7201 cctcatttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat ggcaacgttc
     7261 gtaagctacg ctgggtgttt ataaccggta gtgcgatccc cctggtggca tatattttct
     7321 ggcaggtggc aacgcttggc agcattgatt caacaacctt tatgggattg ctggctaatc
     7381 atgctggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg
     7441 agctggcagt gcatttattt gctgatttag ccctcgccac gtcatttctc ggcgttgcgt
     7501 taggcttatt tgattatctg gctgatttat ttcagcgttc aaataccgtt ggtggacggt
     7561 tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt tgcactgttt tatccacgag
     7621 gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca ttgattatcc
     7681 cttcgctgtt gacctggcaa agcagaaagc acaatcctca ggcgggttac cgggtcaaag
     7741 gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat
     7801 ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc aaattcctta
     7861 ttgggtgcca gaattaacgc tgacacccaa tttggcctct taatgcaggc agcactgctt
     7921 aaatttctta ccactaccgc acgggcaagg atcgttacgc cccggtttct cttctgcttt
     7981 gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtacgcat gtagatcaag
     8041 cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca tcttttctac
     8101 ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg gttttaacga
     8161 atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa agcaccactc
     8221 ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg gctcaaactg
     8281 ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag ccaggttcat
     8341 aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc caccccacac
     8401 ggcaaccagc cactgttccg gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa
     8461 accgtccagc tccgccacat caaggatggc gtggttagtg ttgtatttgg tcagaatatc
     8521 gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg
     8581 gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata ctttttgagt
     8641 ggctttgccg gtgattaaaa attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa
     8701 ccacccgaag gtggtttcac gacactgctt attgctttga ttttattctt atctttccca
     8761 tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt ttaaatccct
     8821 tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg
     8881 aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc gccaaattct
     8941 tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg agcgggaaac
     9001 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
     9061 cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca atcagtgccg
     9121 ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat ttttcaccac
     9181 ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa tctgcacgcg
     9241 cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca aagaaaagtg
     9301 caataccggc gtactcaacc caaatgttcg gacgccacag cagccatgcc aacgccacca
     9361 tctgggcagt ggttttcact ttcccaatcc aggagacagc cacgctactg cgtttaccca
     9421 actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc atcgttgctg
     9481 ccggtaaggt cacccaccag ctgtggtaat gctcggttac cagcaccatg gcgatagcca
     9541 cgagaacttt atctgctaca gggtcaagga aagcaccaaa ccgggtactc tggttccagc
     9601 ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg
     9661 cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat gggataagga
     9721 tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg ggtaactatc
     9781 tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca acgaccagaa
     9841 gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct cgacgctggc
     9901 gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac gttttggccc
     9961 gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccgtt ttttacggtg
    10021 cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc tggataacat gcagcgcggg
    10081 agaatctggc ggcaaactga atccctcacc ttccggctca aagaacagcg tttccagccc
    10141 agccttacga tctgctcctt tggcaacgcc aagtagcagc ggatgatttt tatcccatga
    10201 gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat
    10261 aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc gacgcagcac
    10321 ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat agcgccgata
    10381 ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg tttgttcgcc
    10441 catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt tcaacacgct
    10501 ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc tggttaaggc
    10561 cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt
    10621 ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca gcgttttatc
    10681 gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa
    10741 atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc caggcacttt
    10801 cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata cgtggacaca
    10861 agccatgccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg tattggaaac
    10921 gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac gtgcagcttc
    10981 ttcaaactcc agattctggc tggcagtttc catacgacta atgagttgcg taagcacctg
    11041 atcatctttg ccagacaaaa acaggcgcac atactcaacc tgctgagcgt attcttcttc
    11101 actcaccagt ccttcaacgc acggtcccag acagcgccct atctgatatt gcagacacgg
    11161 acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa tcttttgcag
    11221 tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat attcaccttt
    11281 ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg gtatcgccac tcaggaagat
    11341 aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat
    11401 gtagttgtgt tccagcaaca gcgcttcggt ttctgtatgc gtaaccgtta catcaatttg
    11461 ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctgc ggaaatagct
    11521 ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac caccagcatc
    11581 gtacatgcga taaacgcctg gctggcaggt tacggttttt aaaaacgctt ttgcgtcaaa
    11641 ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg aattgccagg
    11701 tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata gcggtagctg
    11761 ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt ctggcccttg
    11821 gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg gctttctgtt
    11881 ttttctggtt cgatctggct taacgccatt tgttgagcga tatcagaagc aatgtaacgc
    11941 tgccctgaat agacagaacg aatcgcactc acgacttcct gcggagccgc gcctttgctg
    12001 aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc tgtatggacg
    12061 gtaagcatga tgattttgac atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga
    12121 ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt tgtccggcac
    12181 cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc ctttatatct
    12241 tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag tagaacgttg
    12301 atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca ttcgtatatt
    12361 agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt
    12421 ttttgtaaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc ttccgggatc
    12481 gtttcacagt aagcccggcg atgaaatgtg acaaaaattg actttattca gcaaaaatga
    12541 aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca aaaaacattc
    12601 atgataaaaa tatttattgg tcattatatt aacgcattta atagcattgt tgacgtcacg
    12661 ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc cgccttaaca
    12721 tttttcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta cgcctgattt
    12781 ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga aagcgatgct
    12841 gacgctgatg ctacaggcga tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga
    12901 aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca aaacggttaa
    12961 gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgagagcgtg cctttcagcc
    13021 gatttttgca tctggcacgc agaaagaaaa gcgatcaaat taagccagta gcggccgcgt
    13081 aacaggccac ctgggtctta tttggtgcat tgattttttt ctgcatgttt ttctgatgga
    13141 aattgaccgt attctcagag attgacaaaa tcatcgctat ctctgctgat gtcttccctt
    13201 ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa attcatctct ggcgtcatca
    13261 ctatttcatc atttaaacgc atcagagcca tcagactttc gcgcaccagt aactgcattt
    13321 ttaattgcag ttcatcacta agaatgggta tttcgcgcgt gctgcaacgg gaaaaggaca
    13381 aaaagcccag cgcccgattt ggcagcatta aatactgagt gacaccgcgg cgtaaaccat
    13441 gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa taagtcatca ttccacatta
    13501 aatggccctg actaaagttt tcagggttca gcacaggatc aattgcgaga aaattttttg
    13561 cctgataata actaacccac gactcagggt aattggtgta gaaagccact ttaggtcgcg
    13621 tgaatggtac cgggtggcga acacataacg aatagtaatc gtactccagc tgctgagcct
    13681 gaagctctat ttcatggtag acctcttctg cggcctccat cctctgaaaa cgcaacagca
    13741 tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt aaaccgcaac gcccctgaga
    13801 gtgagtatca tttataatga taattcaaac tatcacataa ataagattta tatataattt
    13861 atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg ttattaatga
    13921 gttatgctga tttgttaagc agttttatca cgcttgaaat ggcgtcccgc cccgacaggt
    13981 gaatcgtcgg ggctgatttt ttcttattat tgcagtagaa acttctcgag gaactggcgg
    14041 gtgcgaggct gctggggatc agcaaataac gcttttgctg ccccctgctc gactatccgc
    14101 ccctggtcca taaaaatcgc ccggtccgca acatcccggg caaagctcat ttcgtgcgtc
    14161 acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga ttgtgttcag gacttcgccc
    14221 accagctctg gatccagcgc tgacgttggc tcgtcaaaca aaatcacctc aggacgcatt
    14281 gccagcgcac gcgcaatcgc tacacgctgc tgttgaccgc cagacaaacg acgtggatag
    14341 ctggtttctt taccggccag cccaactttc gccagcagct cgcgagcgcg cgccgtggcc
    14401 tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc cagcaccgta
    14461 cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg caactggcgg
    14521 atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc gccaacggtg
    14581 atcgttcccg cttccggttg ttccagcaga tttatgctgc gtagcaacgt ggttttgccg
    14641 gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag gtcgataccg
    14701 tgcagcaccg tctgaccgtg aaattttttc accaggttct taacttcaat ggcactcatt
    14761 ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac gtcgataata
    14821 ctgtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac acctccagcg
    14881 tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt gtcgcggcca
    14941 gcgacgtatc ctttaccagg ctgataaagc tgttcgacag cggcggcaac gccacccgcg
    15001 ccgcctgtgg caaaatcgcg cgacgcatag tctgccatgg cgtcatacca atactggcag
    15061 ctgcttccca ctgaccttta tcaatagaag aaatggcggc gcgcagtgtt tcggcggcat
    15121 aggcggcagt gttcagcgac agaccaatca ttgctgacgg aatgggatct aattcgatac
    15181 caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggt gtaccacgaa
    15241 aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac aggcgcatca
    15301 gcgcgagaat aaacccgagc agtaagccaa aaaacatgcc gccgatactg agttgcagcg
    15361 tatacccagc ccctttcagc aggaacggca aggaatcaat aaccagttgt atactttctt
    15421 gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag gcgcaccgcc
    15481 ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga tacagccaat
    15541 cagccccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt ccagccgcgc
    15601 cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata
    15661 gtcatcccag agtataattt ccgctgatgc ggtcagctcc agttctttcg caatcgcctg
    15721 ttgtaggtta accactttcg gcaactgatc ggcaacggaa cgcgacacgg tcacgccaat
    15781 cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt gagttccggc
    15841 actgcccgat gcgactacca ccgacgaaat attaaccgcc ccttcacact gttgcgcgat
    15901 ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc caaccggaat
    15961 gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt
    16021 gggatcggtc agtgcttcgc acatttcaat ctgggtattg aacagatcca gcaacaagcg
    16081 attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca gcagcgccac
    16141 acagtgcaga ccgagtttcg ccgcaactgc ggcagtctgc cgcacatggt tagactggat
    16201 caccccggca gtaatcagcg tatcggcacc ttcgcgcaga gcatctgccg cgagaaattc
    16261 tagcttacgt aatttattgc cgcccattgc catgggggtg acgtcatccc gtttgatgaa
    16321 aatttcccgt cctagataat cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc
    16381 gataaactcc aaccgtggaa aacgggttaa attatgcagt ggcataacag cctccgatgt
    16441 atgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa agg
//
LOCUS       DAJVXP010000091        16392 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVXP010000091 DAJVXP010000000
VERSION     DAJVXP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.091"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OQS10_002789"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OQS10_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS2293774.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OQS10_002790"
     CDS             complement(2362..2802)
                     /locus_tag="OQS10_002790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2293775.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQS10_002791"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OQS10_002791"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS2293776.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OQS10_002792"
     CDS             3520..4218
                     /locus_tag="OQS10_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2293777.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OQS10_002793"
     CDS             4447..5655
                     /locus_tag="OQS10_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2293778.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OQS10_002794"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OQS10_002794"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS2293779.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OQS10_002795"
     tRNA            7762..7838
                     /locus_tag="OQS10_002795"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="OQS10_002796"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="OQS10_002796"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="OQS10_002797"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="OQS10_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS2293780.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="OQS10_002798"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="OQS10_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS2293781.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="OQS10_002799"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="OQS10_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS2293782.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="OQS10_002800"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="OQS10_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS2293783.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="OQS10_002801"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="OQS10_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS2293784.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="OQS10_002802"
     CDS             complement(14622..15893)
                     /locus_tag="OQS10_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS2293785.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3898 a         4088 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    16201 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    16261 cccccgcacg ttgcaggtat gtacctgaca acataacggg ggctaatctt gaacctagac
    16321 cactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
    16381 gagctgggca tg
//
LOCUS       DAJVXP010000092        16341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVXP010000092 DAJVXP010000000
VERSION     DAJVXP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.092"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..578
                     /locus_tag="OQS10_002803"
     CDS             <1..578
                     /locus_tag="OQS10_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS2293786.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="OQS10_002804"
     CDS             581..1744
                     /locus_tag="OQS10_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS2293787.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="OQS10_002805"
     CDS             1828..3453
                     /locus_tag="OQS10_002805"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS2293788.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OQS10_002806"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OQS10_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS2293789.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OQS10_002807"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OQS10_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS2293790.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="OQS10_002808"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="OQS10_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS2293791.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OQS10_002809"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OQS10_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS2293792.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OQS10_002810"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OQS10_002810"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS2293793.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="OQS10_002811"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="OQS10_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="OQS10_002812"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="OQS10_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS2293794.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="OQS10_002813"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="OQS10_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS2293795.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="OQS10_002814"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="OQS10_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS2293796.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="OQS10_002815"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="OQS10_002815"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS2293797.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="OQS10_002816"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="OQS10_002816"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS2293798.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="OQS10_002817"
     CDS             12037..12588
                     /locus_tag="OQS10_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS2293799.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="OQS10_002818"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="OQS10_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="OQS10_002819"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="OQS10_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS2293800.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="OQS10_002820"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="OQS10_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS2293801.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="OQS10_002821"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="OQS10_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS2293802.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="OQS10_002822"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="OQS10_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS2293803.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="OQS10_002823"
     CDS             complement(14856..15827)
                     /locus_tag="OQS10_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCS2293804.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..16296)
                     /locus_tag="OQS10_002824"
                     /pseudo
     CDS             complement(15880..16296)
                     /locus_tag="OQS10_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3839 a         3987 c         4342 g         4173 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatgacggt aatgactcca acttattgat
    16321 agtgttttat gttcagataa t
//
LOCUS       DAJVXP010000093        16182 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVXP010000093 DAJVXP010000000
VERSION     DAJVXP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.093"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(137..1165)
                     /gene="malI"
                     /locus_tag="OQS10_002825"
     CDS             complement(137..1165)
                     /gene="malI"
                     /locus_tag="OQS10_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS2293805.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1340..2932
                     /gene="malX"
                     /locus_tag="OQS10_002826"
     CDS             1340..2932
                     /gene="malX"
                     /locus_tag="OQS10_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS2293806.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2942..4114
                     /gene="malY"
                     /locus_tag="OQS10_002827"
     CDS             2942..4114
                     /gene="malY"
                     /locus_tag="OQS10_002827"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS2293807.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4218..5219
                     /gene="add"
                     /locus_tag="OQS10_002828"
     CDS             4218..5219
                     /gene="add"
                     /locus_tag="OQS10_002828"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS2293808.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5255..6295)
                     /locus_tag="OQS10_002829"
     CDS             complement(5255..6295)
                     /locus_tag="OQS10_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2293809.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6539..6664
                     /gene="blr"
                     /locus_tag="OQS10_002830"
     CDS             6539..6664
                     /gene="blr"
                     /locus_tag="OQS10_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS2293810.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6929..7144
                     /gene="ydgT"
                     /locus_tag="OQS10_002831"
     CDS             6929..7144
                     /gene="ydgT"
                     /locus_tag="OQS10_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS2293811.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7230..7670
                     /locus_tag="OQS10_002832"
     CDS             7230..7670
                     /locus_tag="OQS10_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS2293812.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7747..8328
                     /gene="rsxA"
                     /locus_tag="OQS10_002833"
     CDS             7747..8328
                     /gene="rsxA"
                     /locus_tag="OQS10_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS2293813.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8328..8906
                     /gene="rsxB"
                     /locus_tag="OQS10_002834"
     CDS             8328..8906
                     /gene="rsxB"
                     /locus_tag="OQS10_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS2293814.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8899..10833
                     /gene="rsxC"
                     /locus_tag="OQS10_002835"
     CDS             8899..10833
                     /gene="rsxC"
                     /locus_tag="OQS10_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS2293815.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10834..11892
                     /gene="rsxD"
                     /locus_tag="OQS10_002836"
     CDS             10834..11892
                     /gene="rsxD"
                     /locus_tag="OQS10_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS2293816.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11896..12516
                     /gene="rsxG"
                     /locus_tag="OQS10_002837"
     CDS             11896..12516
                     /gene="rsxG"
                     /locus_tag="OQS10_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS2293817.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12520..13215
                     /gene="rsxE"
                     /locus_tag="OQS10_002838"
     CDS             12520..13215
                     /gene="rsxE"
                     /locus_tag="OQS10_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS2293818.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13215..13850
                     /gene="nth"
                     /locus_tag="OQS10_002839"
     CDS             13215..13850
                     /gene="nth"
                     /locus_tag="OQS10_002839"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS2293819.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14461..15963
                     /gene="dtpA"
                     /locus_tag="OQS10_002840"
     CDS             14461..15963
                     /gene="dtpA"
                     /locus_tag="OQS10_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS2293820.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16068..>16182
                     /locus_tag="OQS10_002841"
     CDS             16068..>16182
                     /locus_tag="OQS10_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS2293821.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3762 a         4116 c         4125 g         4179 t
ORIGIN      
        1 gtctcaagtt gtcagaatta aacacataaa cctcctgtga acttcagtaa aaataaaagg
       61 caccgcctgt gtgccacatt ttttatcatt aaccttgagg aaaggacact accaggtata
      121 gcagataaag agaggattat ttcgctgcaa tgagccgggc gggaataata agattgcgtg
      181 aatgcgtctc ttcatgggtg attttttgca tcatgcgatc cgcaagtgtg gtaccaagtt
      241 cccgcgcagg cgtgctggcc caggtaacgg gtatatcatc aagtgtggtt ggtgtcgcat
      301 cggtaaatgc cgccagcgaa acttgttgct caaagtaacg atcgactccg ctttccccgc
      361 tttgccgccc tgctttcagc aaaccaaacc atgcccccat cgcaatagtt tcgttatagc
      421 aaaccacagc actgatggtc gggttatgac gtaaaagcgc cgtgatagct tccgcggctt
      481 gcttctggct ggatgtgcac tccagcaccc aatcgctgtg aaacggcagg ccaaatttta
      541 gtagagttgc acaatagcct cccacccgtt ctgcccgggt taatgaggaa ctttgccctc
      601 ccagccaggc gatccgctga tgcccattgc gaatgagatg ctccgtcaac aactgtgcag
      661 cctgcatgtt gtccgggcga accgtatcaa catcatcaag ataactggca cgggaagcga
      721 aaatcaccgg gatagctttt tcttctgcca tccgtcgcag gtcatcgctg cttcctgcag
      781 ccccggcaat taccacacca tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca
      841 gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat ccgtccctgc gcttccagag
      901 cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc agataaatca cggacgatca
      961 aaccaatgac gccgctttgc ccgccgcgca gcgccgacgc ctggcgattg cgcacaaatc
     1021 ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc
     1081 cactaagcac cagcgaaacg gtacttaccg acacgcccgc agccagcgca acatcatgaa
     1141 tggttatttt tttggcggta gccatgcaga tgacctactc cctgattatg tgacagataa
     1201 aacgttttac cttttattta tcttataccc gctattatcg ttgcgtaatg tgatttatgc
     1261 ttcactaaaa tttgataaaa cgttttatct tctcgcgcaa tttactgaat ccagattgtt
     1321 ctctacgagg agtcgtttta tgacggcgaa aacagcaccg aaagtcacgc tgtgggagtt
     1381 cttccagcag ttaggcaaaa ccttcatgtt acccgtggca ttattgtcgt tctgcggcat
     1441 tatgctcggc attggtagtt ctcttagtag ccacgatgtc atcaccctga tcccggtcct
     1501 gggcaacccc gtgttgcagg ctatctttac ctggatgagt aagattggct cgtttgcttt
     1561 tagtttcctg ccagtcatgt tctgtatcgc catcccgctg ggtctggcac gcgaaaacaa
     1621 aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt
     1681 ctggttgacc aataaaggca ttctgccaac cacggacgcc gcggttctga aagccaataa
     1741 catccagagc attcttggga tccagtcgat cgacaccggg atcctcggtg cggtgatcgc
     1801 cggtattatc gtctggatgc tgcatgagcg attccataat atccgcctgc cggatgcgct
     1861 tgcattcttc ggcggtacgc gcttcgtacc aatcatctcc tcgctggtga tgggccttgt
     1921 cggcctggtg attccattag tctggccgat tttcgcgatg ggtattagcg gcttgggcca
     1981 tatgataaac agcgcgggtg atttcggacc gatgctgttt ggtaccggtg aacgtctgct
     2041 gttgccgttt ggtctgcatc acattctggt ggcattaatt cgctttaccg acgcaggcgg
     2101 cacgcaggaa gtctgcggtc aaaccgtcag cggcgcattg accatcttcc aggcgcaatt
     2161 gagttgcccg accactcacg gtttttctga aagcgccacg cgtttccttt cgcaaggcaa
     2221 aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta gcgatgtatc actgcgcgcg
     2281 cccggaaaat cgccataaaa ttaaagggct gctgatttct ggcctgatcg cctgtgtcgt
     2341 tggcggcacg accgaaccgc tggaattcct gttcctgttc gtggcgccag ttctgtatgt
     2401 catccacgcg ctgttaaccg gcctcggctt cactgtcatg tctgtgctcg gcgtcaccat
     2461 cggtaatacc gacggcaata tcatcgactt cgtggtgttc ggtattttgc atggtctgtc
     2521 aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg tttgtcgttt actacgtcat
     2581 cttccgtttc gctatcaccc gcttcaatct gaaaaccccg gggcgcgata gcgaagtggc
     2641 cagttcaatc gaaaaagccg ttgccggtgc gccgggtaaa tcaggttaca acgttccggc
     2701 aatcctcgaa gccttaggcg gtgccgacaa tattgttagc ctcgataact gcattacccg
     2761 tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg caggcactga aggacaatcg
     2821 ggcaattggc gtagtacaac ttaatcaaca taacctgcag gttgttatcg ggccacaagt
     2881 tcagtcagta aaagatgaaa tggccggtct gatgcatact gtccaggcat aaggataaga
     2941 tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca tggtgtacac agtgggatta
     3001 tgtcgctgac cgtttcggca ctgctgacct gttaccgttc acgatttcag acatggattt
     3061 tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc ctgatgcacg gcgtatttgg
     3121 ctacagccgc tggaaaaacg atgagtttct cgcggctatt gcccactggt tttccaccca
     3181 gcattacacc gcaattgata cgcagacggt ggtgtatggc ccttctgtca tctatatggt
     3241 ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc gtggtgatcc acacgcccgc
     3301 ctatgacgca ttttacaagg ccattgaagg taaccagcgc acagtaatgc ccgttgcttt
     3361 agagaagcag gctgacggct ggttttgcga tatgggcaag ctggaagccg ttttggcgaa
     3421 accagaatgt aaaattatgc tcctgtgtag cccacagaat cctaccggga aagtgtggac
     3481 gtgcgatgag ctggagatca tggctgacct gtgcgagcgt catgatgtgc gggttatttc
     3541 cgatgaaatc catatggata tggtctgggg cgagcagccg catattccct ggagtaatgt
     3601 ggcgcgggga gactgggcgt tgctgacgtc gggatcgaaa agtttcaata ttcccgcgct
     3661 gaccggtgct tacgggatta tagaaaatag cagcagccgt gatgcctatt tatcggcact
     3721 gaaaggacgt gatgggcttt cttccccttc ggtactggcg ttaactgccc atatcgccgc
     3781 ctatcagcaa ggtgcaccgt ggctggatgc cttacgcgtc tatctgaaag ataacctgac
     3841 gtatatctca gataaaatga acgccgcgtt tcctgaactc aactggcaga tcccgcaatc
     3901 cacttatctg gcctggcttg atctacgtcc gttgaatatt gacgacaacg cgttgcaaaa
     3961 agcgcttatc gagcaagaaa aagtcgcgat catgccgggg tatacctatg gtgaagaagg
     4021 tcgtggtttt gtccgtctca atgccggctg cccacgttcg aaactggaaa aaggtgtggc
     4081 tggattaata aacgccatcc gcgctgttcg ctaaccccaa ttgcgcaacg taaaaaatcg
     4141 ttgcgcaatc gtggattttt accctgcttt gtttttataa tggtgcgcac ttttatatcc
     4201 agaaaaagag tgcgaccatg attgatacca ccctgccatt aactgatatc catcgccacc
     4261 ttgatggcaa cattcgtccc cagaccattc ttgaacttgg ccgccagtat aatatctcgc
     4321 ttcctgcaca atccctggaa acactgattc cccacgttca ggtcattgcc aacgaacccg
     4381 atctggtgag ctttctgacc aaacttgact ggggcgttaa agttctcgcc tctcttgatg
     4441 cctgccgccg cgtggcattt gaaaacattg aagatgcagc ccgtaacggc ctgcactatg
     4501 tcgagctgcg tttttcacca ggctacatgg caatggcaca tcagctgcct gtagcgggtg
     4561 ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga
     4621 agcttatcgg cattatgagc cgaaccttcg gcgaagccgc ctgtcagcaa gagctggagg
     4681 cctttttagc ccaccgtgac cagattaccg cacttgattt agctggtgat gaacttggat
     4741 tcccgggaag tctgttcctt tctcacttca accgcgcgcg tgatgcgggc tggcatatta
     4801 ccgtccatgc aggcgaagct gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg
     4861 gtgcggagcg tattggacat ggcgtaaaag ccattgaaga tcgggcgctg atggattttc
     4921 tcgccgagca acaaattggt attgaatcct gtctgacctc caatattcag accagcaccg
     4981 tagcagagct ggctgcacat ccgctgaaaa cgttccttga gcatggcatt cgtgccagca
     5041 ttaacactga cgatcccggc gtacagggag tggatatcat tcacgaatat accgttgccg
     5101 cgccagctgc tgggttatcc cgtgagcaaa tccgccaggc acagattaat ggtctggaaa
     5161 tggctttcct cagcgcagag gaaaaacgcg cactgcgaga aaaagtcgcc gcgaagtaac
     5221 aaaaatggat ggtgcaattt gcaccatcca tttttcacgc aaggcacaac gtcgcgcgat
     5281 gtttggcgga ttcgatgccc agctcaatca actccattac ctggattgcc tggcttgccg
     5341 gaaccggatt ttcaccatcg ccatttaacg catcacgaat cgccgcatag taagccggat
     5401 aattcccagg caccgtcaac agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa
     5461 gtacgccatc acgcatatcg tagccccagt cttcctgcgg cagacgctcg ccatttttca
     5521 gacgttcttc ctgtggatcg aggccatatt tcacacaact gcctcgggat ccatgcacga
     5581 tataccgtgc tgactcagcg gctgccagca tggtaccgtg taaaatgact cgccgctgtg
     5641 gataggacaa gatggcgtgg aaataatcgg tcgactgcgc tccgggccgt aactgtgcca
     5701 aatctaccgt catgctgacc ggtaaaccaa atagcgtaat ggcctgatca agaagatgcg
     5761 gtgctaaatc gtaccagata ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca
     5821 cctgcggacg gaagcggtca aaatgagact caaagtaagc aacttcaccc agcacgcctt
     5881 ccgcgagcaa accttttagc gtcaagaaat cgctatccca acgacggtta tggaatacag
     5941 acagcacacg ccccaggctt tttgccagcg catccagctc tcgcgcttgt gacagtgtca
     6001 cggtaaaggg tttatcaacg accacatgtt tacccgcctc aagcgccgct ttggctaacg
     6061 ggaaatgggt atcgttgggt gtaggaatga caatcaggtc tatgttggga tcgttaaaca
     6121 gatgcttcgg ctcagagaca accgtaaccg ttggccagtc ggcttttact tttgtttcat
     6181 cactgctgga gattaccgcc agttccagcc cgggcgtgcc cgcaatcagg ggcgcatgga
     6241 aggttttgct cgcataacca tacccaatca acccaacacg gatgttgtcg ctcatgtcat
     6301 tgcctctcat tgaagtatga tggctatttg acactatcct ttacccacgc tcaacagttt
     6361 aataacctgc cagcaataag ggatgttgtt taacttaagt caaaaaaaat agcgaatttt
     6421 ccaacgacaa aagctaaata tcgcaaaaac ctcagtaaaa atcttgctgg agctattatt
     6481 gctaagtaac atttaccccc tgaagttaat ggatcaatca agagagatgt gggctgtaat
     6541 gaatcgtctt attgaattaa caggttggat cgttcttgtc gtttcagtca ttcttcttgg
     6601 cgtggcgagt cacattgaca actatcagcc acctgaacag agtgcttcgg tacaacacaa
     6661 gtaagctctg cacttgtgga gcgacatgct gcccgtccgg gtgcatgttt tcacttgtcg
     6721 gatattaaac caggaattta ttatcttgtt cgatgttgtt ggtgattgtc agggatagta
     6781 aagcgcgtta ctcttttcag agcagttttt catccctgaa tatttgccat cagcgcattc
     6841 ttattattgt aataacacaa gcgttcactt ttggtgaaat tttatcatca ctctacccct
     6901 ttggtattaa ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg
     6961 caaaatcagt tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac
     7021 ggacgatcag gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt
     7081 ttctggcggg cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca
     7141 ataaagaaag tagcgatcgg ttataaaagc cttataataa cgcctctaaa attagatttg
     7201 cctgtttgcg tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag
     7261 gctggttgct tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt
     7321 tgttgctgta taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag
     7381 cactgacgac acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt
     7441 acacactgtg gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata
     7501 ttatctggct gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg
     7561 aagatggcat agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc
     7621 cctatttcaa acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca
     7681 gttaacctgt tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc
     7741 cgaaaaatga ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta
     7801 ctggtcaagt ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg
     7861 atgggcatgg ggctggcaac aacgtttgtg atgacgctgg cgtctatttg cgcctggctt
     7921 atcgatacgt ggattttgat cccacttaat ctgatttacc tgcgcaccct ggcatttatt
     7981 ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg
     8041 ctttaccgtc tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc
     8101 gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt
     8161 tccgccgctg tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt
     8221 gctgtggctg atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt
     8281 cttatgtctc tggcctttat gggctttaat ggtttggtga agttgtaatg aatgctatct
     8341 ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg
     8401 cctcccgccg ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac
     8461 cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca
     8521 gctgtaacgg tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa
     8581 ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc
     8641 ctgcgcggat ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc
     8701 aggcttgtcc ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg
     8761 atctctgtac gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc
     8821 aaccggtcgc agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta
     8881 tcattcccgt ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg
     8941 gatttcaacg gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacacccctg
     9001 cgccaggtac cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa
     9061 ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt
     9121 cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc
     9181 cactctacgg ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt
     9241 gaagactgct ggatcccgcg cgacggctgg gccgattatc gcactcgcag tcgcgaagag
     9301 ttaatcgagc gcatacatca gtttggtgtt gccgggctgg gcggtgcagg attcccgaca
     9361 ggcgttaaat tgcagggtgg cggagataag attgaaacgt tgattatcaa cgcggctgag
     9421 tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta
     9481 gagggtattc gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa
     9541 gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt
     9601 tctctgcggg tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt
     9661 ctgaccggga agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa
     9721 aacgtcggca ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag
     9781 cgtgttgtaa ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg
     9841 gggacgccag tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg
     9901 gtgattatgg gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta
     9961 aagattacca actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa
    10021 caaagctgca tccggtgtag cgcctgtgct gacgcctgcc ctgcggatct tttgccgcaa
    10081 cagttgtact ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgct
    10141 gattgcattg aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cctggtgcaa
    10201 tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca
    10261 gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc
    10321 cttgaacgac ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct
    10381 gccgctctgg cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa
    10441 gcgggcgaac gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa
    10501 gccagagcga aacaggcaga actgcagcaa actaacgacg cagcaaccgt tgctgatcca
    10561 cgtaaaactg ccgttgaagc agctatcgcc cgcgccaaag cgcgcaagct ggaacagcaa
    10621 caggctaatg cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagccgct
    10681 attgcccgtg ccaaagcgcg caagctggaa cagcaacaga ctaacgcaga accagaagaa
    10741 caggttgatc cgcgcaaagc ggcagttgcc gcggctattg cccgcgctca ggccaaaaaa
    10801 gccgcccagc agaaggttgt aaacgaggac taaatggtat tcagaatagc tagctcccct
    10861 tatacccata accagcgcca gacatcgcgc attatgctgt tggtgttgct cgcagccgtg
    10921 ccaggaatcg cagcgcaact gtggtttttt ggttggggta ctctcgttca gatcctgttg
    10981 gcatcggtta gtactctgtt agccgaagct ctcgtactca aactacgcaa gcagtcggta
    11041 gccgcaacgt tgaaagataa ctcagcattg ctgacaggct tattgctggc ggtaagtatt
    11101 ccccccctcg cgccatggtg gatggtcgtg ctgggtacgg tgtttgcggt gatcatcgct
    11161 aaacagttgt atggcggtct ggggcaaaac ccgtttaatc cggcaatgat tggttatgtg
    11221 gtcttactga tctccttccc ggtgcagatg accagctggt taccgccaca tgaaattgcg
    11281 gtcaacatcc ctggttttat cgacgccata caggttattt tcagcgggca taccgccagt
    11341 ggtggtgata tgaacacact acgcttaggt attgatggca ttagtcaggc gacaccgctg
    11401 gatacattta aaacctctgt ccgtgccggt cattcggttg aacagattat gcaatatccg
    11461 atctacagcg gtattctggc gggcgctggt tggcaatggg taaatctcgc ctggctggct
    11521 ggcggcctgt ggttgctatg gcagaaagcg attcgctggc atattcccct cagcttctta
    11581 gtaacgctgg cgttatgcgc aacgttgggc tggttgttct caccagaaac actggcagca
    11641 ccgcaaattc atctgctgtc tggtgcgacc atgctcggcg cattctttat tttgactgac
    11701 ccggttaccg cttctacgac caatcgtggt cgtcttattt tcggcgcgct tgcgggctta
    11761 ttagtctggt tgatccgcag tttcggcggc tatcctgacg gcgtggcttt tgccgtcctg
    11821 ctggcgaaca tcacggttcc tctgatcgat tactacacgc gtccgcgcgt ctacggccat
    11881 cgcaaagggt aaaccatgct gaaaactatc cgaaaacacg gcattacgtt agcgctattt
    11941 gcagcgggtt caacagggtt aactgcggcc atcaaccaga tgaccaaaac gacgattgct
    12001 gaacaggcca gtctgcaaca aaaggcatta tttgatcagg tgctgccagc cgaacgctat
    12061 aacaatacgc tggcacagag ttgctatctg gtaactgcgc cagagttagg taaaggtgag
    12121 catcgggttt acatcgccaa acaggatgac aaaccggtag ccgccgttct ggaagcaacc
    12181 gcgccagatg gctattccgg tgcgattcag ctgctggtgg gagccgattt taacggcacg
    12241 gtacttggca cgcgcgtgac agagcaccac gaaacgccag ggcttggcga taaaatcgaa
    12301 ctgcgccttt ctgactggat cacccatttt gcgggtaaaa aaatcagtgg tgcggatgat
    12361 gcgcactggg cggtgaagaa agatggtggt gatttcgacc agttcaccgg cgcgacgatt
    12421 actccccgcg cggtggttaa tgcggtaaaa cgcgccggat tgtacgctca gacgttaccg
    12481 gcacaacttt ctcaacttcc tgcctgtgga gaataaagcg tgagcgaaat taaagacgtt
    12541 attgttcagg ggttgtggaa aaacaactct gcgctggtcc agttgctcgg cctttgtcct
    12601 ctgttggcgg tcacgtccac tgccactaac gctctgggtt taggacttgc gactacgctg
    12661 gtactgacgc tgaccaacct gaccatttcg acgctgcgtc actggacgcc agccgagatc
    12721 cgcattccca tttacgtgat gatcatcgcc tcggtggtca gcgctgtaca gatgctgatc
    12781 aacgcctacg cctttggcct gtatcaatca ttagggattt ttattccgct gattgtcact
    12841 aactgtatcg ttgtgggccg cgctgaagcc ttcgccgcca aaaaaggtcc ggcgctttcg
    12901 gcactggacg gcttttcaat tggtatgggc gcaacctgcg ccatgttcgt gctgggttca
    12961 ctacgcgaaa ttatcggcaa tggcacattg tttgacggtg cagatgcgct gttaggtagc
    13021 tgggcaaaag tattacgcgt ggagattttc cacaccgact cccctttcct gctggcgatg
    13081 ctgccaccag gtgcatttat tggcctggga ctgatgctgg caggaaaata cctgattgat
    13141 gaaagaatga aaaagcgccg tactgaagca gctgccgaac gtgcattgcc aaacggtgaa
    13201 acagggaatg tctgatgaac aaagcaaaac gcctggagat cctcactcgc ctgcgtgaga
    13261 acaatcctca tcccaccacc gagcttaatt tcagttcgcc ttttgaattg ctgattgccg
    13321 tactgctttc cgctcaggcg accgatgtca gtgttaataa ggcgacggcg aaactttacc
    13381 cggtggcgaa tacgcctgca gcgatgcttg aactgggcgt tgaaggggtt aaaacctata
    13441 tcaaaacgat tgggctttat aacagcaaag cagaaaatat catcaaaacc tgccgtatct
    13501 tactggagca gcataatggc gaggttccgg aagatcgtgc tgcgcttgaa gccctgcccg
    13561 gcgtaggtcg taaaacagcc aacgtcgtat taaacactgc attcggctgg ccgactattg
    13621 ctgtcgacac gcacattttc cgcgtttgta atcgtactca atttgcgccg gggaaaaacg
    13681 tcgaacaggt agaagaaaag ctactgaaag tggttccggc agagtttaaa gtcgactgcc
    13741 accattggtt gatcctgcac gggcgttata cctgcattgc ccgcaagccc cgctgtggct
    13801 cttgtattat tgaagatctt tgtgaataca aagagaaagt tgacatctga agaaaagggg
    13861 taacaccgat taccccattg ataacctttc tttatcctct tttaaaacat ctttttaacg
    13921 tgaatgatgc cattgcttag cgttatcatc aggtaatccg tttgcggata accaaaaatg
    13981 caggttaatt gtttttttaa tagcgaaatt ttctattcat tcgtgatcaa gatcacgatg
    14041 ctgtcgcaat gtaaaaacat tgcaagaatc aggttaaaat aatttaaatt tacacacgca
    14101 acaaatattg acctacaaaa cattacactg gctatttttc agaaactgga ctatctcact
    14161 agtaaaaacg ctaaatagca gaacatatcg ccgttaagcc atcacccccc tctcagtgca
    14221 gtgaaaaaat ctgccattac gttttttgaa aaatttaacg ctggataaca tttcccggaa
    14281 tggttgaatt ccccgcctca gttatatgta acagattatt acaaaggact tgtctgaaag
    14341 tgcaagatag tgaacattac ctgccgtttc ccctcccact ataacaattg cgcgtatgtt
    14401 ttttagacat aacgcgagaa agcacccccg ttaatatggg atgtaaaaaa agaggtaaaa
    14461 gtgtccactg caaaccaaaa accaactgaa agcgtcagtt tgaacgcttt caaacaaccg
    14521 aaggcgttct atctcatctt ctcgattgag ttatgggaac gttttggtta ttacggccta
    14581 caaggaatta tggctgttta cctggttaaa caactgggta tgtctgaagc ggattcaatc
    14641 acccttttct cttcctttag tgccctggtt tatggtctgg tcgctatcgg cggctggtta
    14701 ggtgacaagg tactgggtac taaacgcgta attatgctcg gtgctattgt gctggcgatt
    14761 ggttatgctc tggttgcctg gtctggtcac gacgccggta tcgtttatat gggtatggcg
    14821 gctattgcgg tcggtaacgg cctgtttaaa gctaacccat cttctctgct ttctacatgc
    14881 tatgagaaaa acgacccgcg tctggacggt gcattcacca tgtactacat gtccgtcaac
    14941 atcggctctt tcttctctat gattgctacg ccgtggctgg ccgcgaaata cggctggagt
    15001 gttgcgtttg cgttgagcgt tgtaggcctg ctgatcacta tcgttaactt cgccttctgc
    15061 caacgctggg ttaaacagta cggttcaaaa ccagacttcg agcctatcaa ctaccgtaac
    15121 ctgctgctga ccattattgg tgttgtggca ctgatcgcta tcgccacctg gctgctgcac
    15181 aatcaggaag ttgcgcgtat ggcgctgggc gttgttgcct tcggtatcgt ggttatcttc
    15241 ggtaaagaag ccttcgcgat gaaaggtgct gcgcgtcgta aaatgatcgt tgccttcatc
    15301 ctgatgctcg aagccattat cttcttcgtg ctgtacagcc agatgccaac gtcactgaac
    15361 ttctttgcga ttcgtaacgt tgagcactcc attctgggtc tggccgtaga acctgagcag
    15421 tatcaggcac tgaacccgtt ctggatcatc atcggtagtc cgattctggc cgctatctat
    15481 aacaagatgg gcgataccct gccgatgcca accaagtttg caatcggcat ggtgatgtgt
    15541 tctggtgcgt tcctgattct gccgctgggt gcgaaattcg cgtctgacgc tggtatcgtg
    15601 tctgtaagct ggctggtcgc aagctatggc ctgcagagca tcggggaact gatgatctct
    15661 ggtctgggtc tggcaatggt tgcacaactc gttccgcagc gtctgatggg cttcattatg
    15721 ggtagctggt tcctgaccac tgccggtgca aacctgattg gtggttatgt tgcgggtatg
    15781 atggctgtgc cggataacgt taccgacccg ctgatgtcac tggaagttta tggtcgcgta
    15841 ttcttgcaga ttggtgtcgc tactgccatt attgcagtac tgatgctgct gaccgcgcct
    15901 aaactgcacc gtatgacgca ggatgacgct gcagacaaag cggcgaaagc tgctgtagcg
    15961 taaaattcag ggaaactctt ttacaagccg ctaacttttc gttagcggct tttttttgtt
    16021 cagcaacgca ctaccatact ttaaaccaca gacaaaagga gttaccgatg aaattgttct
    16081 acaaaccggg tgcctgctct ctcgcttccc atatcaccct gcgtgagagc ggaaaggatt
    16141 ttaccctcgt cagtgtggat ttaatgaaaa aacgtctcga aa
//
LOCUS       DAJVXP010000094        16075 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVXP010000094 DAJVXP010000000
VERSION     DAJVXP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.094"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..566)
                     /locus_tag="OQS10_002842"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="OQS10_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OQS10_002843"
                     /pseudo
     CDS             complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OQS10_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OQS10_002844"
     CDS             complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OQS10_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS2293822.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3071..3289
                     /locus_tag="OQS10_002845"
     CDS             3071..3289
                     /locus_tag="OQS10_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS2293823.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3297..4238
                     /gene="ribF"
                     /locus_tag="OQS10_002846"
     CDS             3297..4238
                     /gene="ribF"
                     /locus_tag="OQS10_002846"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS2293824.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4281..7097
                     /gene="ileS"
                     /locus_tag="OQS10_002847"
     CDS             4281..7097
                     /gene="ileS"
                     /locus_tag="OQS10_002847"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS2293825.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7097..7591
                     /gene="lspA"
                     /locus_tag="OQS10_002848"
     CDS             7097..7591
                     /gene="lspA"
                     /locus_tag="OQS10_002848"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS2293826.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7716..8165
                     /gene="fkpB"
                     /locus_tag="OQS10_002849"
     CDS             7716..8165
                     /gene="fkpB"
                     /locus_tag="OQS10_002849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS2293827.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8167..9117
                     /gene="ispH"
                     /locus_tag="OQS10_002850"
                     /gene_synonym="lytB"
     CDS             8167..9117
                     /gene="ispH"
                     /locus_tag="OQS10_002850"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS2293828.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9183..10097
                     /gene="rihC"
                     /locus_tag="OQS10_002851"
     CDS             9183..10097
                     /gene="rihC"
                     /locus_tag="OQS10_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS2293829.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10264..11085
                     /gene="dapB"
                     /locus_tag="OQS10_002852"
     CDS             10264..11085
                     /gene="dapB"
                     /locus_tag="OQS10_002852"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS2293830.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11541..12689
                     /gene="carA"
                     /locus_tag="OQS10_002853"
     CDS             11541..12689
                     /gene="carA"
                     /locus_tag="OQS10_002853"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS2293831.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12707..15928
                     /gene="carB"
                     /locus_tag="OQS10_002854"
     CDS             12707..15928
                     /gene="carB"
                     /locus_tag="OQS10_002854"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS2293832.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3922 a         3783 c         4254 g         4116 t
ORIGIN      
        1 tcatcccccc agtagaaagg aatggagata aacagattaa aacgctcctc ttctttatta
       61 ttttcatcat aagattggct ggcagagaaa gtataactaa tgtgttgcca gttattggag
      121 taactgaatt ggtaatcttt agcatttcca cttcgccccc agtaattccg ccaaagcgca
      181 cttaatgata catttcccag attattggat aaaggttgca taatattggc agaaaggcta
      241 ttttttctgc caatatcata aaaatcatca taatcactct gatgatagtg tttatcattt
      301 tcgtaaagat ggtcgctgaa tgtcctgtaa tcctgcgaag cataacgcca ggcggccacg
      361 ctaaatcgag tggctgtttg tactagatat ttattatagg cgacctgata gctcgtacct
      421 tcgtgtctgg tatcattatt taacttacta ctcgatcggg cagcgtaaaa agagatggca
      481 cccagcggtg tattccaacc atttcccaga gttatagcat tatagttatc ggataaaatc
      541 gtacccgatt atgtgatcag gttgttatta tttagattaa tgtttaaatt ttttaatttc
      601 ttgatgtgaa gttgatgaaa gtttgaaaat ttaatattct taattgttcc cttctttata
      661 agaagagcaa ggtgccatca ttagagtgac agagtaggat gtttcagagc atgcttcaga
      721 ttgtttttta tttctctgca gtgtattaat aatcatcaca gataatttca ttctaaaatt
      781 acctctttct ttgagtgttt taatcctgat gaactatatt ttttataagg agcatgcact
      841 cgttgatgct ctgaaggtat tttctcattt ttgatgatgt tttgtcgtaa tcgtgcataa
      901 acttattaag ttctttatac acatcattat ttagctttac attatcgcga actaaagttt
      961 gtaccaatgg tatcaaaatt tcgaaacggg acggtttgtc atcaatactt gccagggttt
     1021 tgaatactcc tgtgtatctg taacctaagt gcttattgac aagttgagtg gtccatcggc
     1081 caggcataat atcttcacag cgcaaatttt ttatcatggt attagtgaac gatgttccaa
     1141 atatgttgct ttttttaatg aagaggttgt ttatttctcc atttgaaaag atggtgtttg
     1201 ataagtcgct ttcaaaacat gatatattgg ttaatacaca acaaaaataa tacccatatg
     1261 aaaggttgct cttttctatt gttatatttg atagcaacat tttatgtata ttaatattaa
     1321 gagcagtcaa atactggatc ttttgatcgt taagttcata catctccagt gatttattat
     1381 agggtaataa aagtataaat tcctttatat tatctccgca gtaagaaaag acgacattaa
     1441 atttattagt ggccatgttt tcagttgagc agtaagctga tttttctatt aaccgactgg
     1501 gcacgctttt aaataaagat aattttccac atagttttga atatgccgaa ttgctagttt
     1561 taaagttctg ccacattatt ccctggttat catctatcga tgaggagggg attttattgc
     1621 attgctcatt gtatagctca gggaaaatat tttttttaat aagctttttg atttcctggt
     1681 taagatgact ttttttcttg tctattgcac tgtattttgc gttttgtgac gtttcctgat
     1741 gatcataaaa tgactggaga tttctgcagg cctcataaaa tatttcagga gtatatagct
     1801 gtgggtatat ttcttggttg aagtttgcct ttaaaaaatt aacgaatttt tcatggcatt
     1861 cttggtatat cttaatctct tcagaaagac attcgcttgt tctgtcatca aaaacatcca
     1921 atctaagtaa tgtgatacct ttgtgtttaa aaaataaaat tgaactgtat gttctatgag
     1981 tttacatcta taaaaatatg catctctcag atgagtatat tgaaaaaaat gatgaaggac
     2041 tggagatggt ttaggcaagt tatggcttct aaaaaagtca tcaaacaaaa ttaagaactc
     2101 tgttttatac tcgcaaaata cttttactaa ctcattttgt cttgttttat ttattattac
     2161 atcatctact atatttgatt tataaagaca atgtggcgaa gttgagatac catttgtcat
     2221 tatcagtcct tgaagttgat gaatttttat taatttaatt tatgagtgaa tgtgactact
     2281 atcaaaaata gctcattaat tttatgaagt agaatataaa taggtattta acctgtgttg
     2341 attgttgtta attatttaca agatctaata aggagatatt tatgaatggt taaagtgtat
     2401 aggaatttaa ttcaaaaata gagtggtctt aagtttaata cctcaatgtg tatcacaata
     2461 tccacattct ttgtagggga gtctggagat tgagtagata ttcttgttca gaatgtatca
     2521 gccgatggtt ctacgattct taagccacga agagttcaga aagtgtaacg gcatgtctgc
     2581 aaaagccagc atcttttgac tatctggcaa ccggtagtgt gttctctcac gcatcataaa
     2641 agcagcaggc ataaaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
     2701 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
     2761 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
     2821 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
     2881 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
     2941 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
     3001 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
     3061 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
     3121 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt
     3181 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
     3241 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
     3301 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     3361 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     3421 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     3481 tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc
     3541 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt
     3601 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg
     3661 ccgtagggga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     3721 aagctagcat ggaatacggc ttcgatatca ctagtacgca aactttttgc gaaggtggcg
     3781 tgcgtattag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
     3841 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     3901 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     3961 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     4021 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     4081 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     4141 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     4201 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     4261 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     4321 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     4381 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     4441 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     4501 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     4561 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     4621 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     4681 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     4741 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
     4801 gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg
     4861 cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt
     4921 ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
     4981 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     5041 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     5101 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     5161 gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg
     5221 acgttccggc aatcctcggc gatcacgtta ccctagatgc cggtaccggt gccgttcaca
     5281 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     5341 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     5401 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     5461 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     5521 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     5581 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     5641 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     5701 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg
     5761 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga
     5821 aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat
     5881 ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc
     5941 acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc
     6001 taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct
     6061 ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc
     6121 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     6181 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
     6241 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag
     6301 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     6361 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     6421 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     6481 gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc
     6541 acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     6601 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     6661 gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga
     6721 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     6781 gctcgctgga agcggcagta accttgtatg cagaaccgga actgtcggcg aaactgaccg
     6841 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     6901 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     6961 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     7021 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     7081 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     7141 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     7201 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     7261 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     7321 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     7381 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     7441 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     7501 caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg tgctggaagg
     7561 ttttttgcct tctagagcga aaaaacaata ataaaccctg ccggatgcga tgctgacgca
     7621 tcttatccgg cctacagatt gctgcgaaat cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaaaa tcctcaaata taagagcaaa cctgcatgtc tgaatctgta cagagcaata
     7741 gcgccgtcct ggtgcacttc acgctaaaac tcgacgatgg caccaccgcc gagtctaccc
     7801 gcaacaacgg taaaccggcg ctgttccgcc tgggtgatgc ttctctttct gaagggctgg
     7861 agcaacacct gttggggctg aaagtgggcg ataaaaccac cttctcgttg gagccagatg
     7921 cggcgtttgg cgtgccgtca ccggacctga ttcagtactt ttcccgccgt gaatttatgg
     7981 atgcaggcga gccagaaatt ggcgcaatca tgctttttac cgcaatggat ggcagtgaga
     8041 tgcctggcgt gatccgcgaa attaacggcg actccattac cgttgatttc aaccatccgc
     8101 tggccgggca gaccgttcat tttgatattg aagtgctgga aatcgatccg gcattggagg
     8161 cgtaacatgc agatcctgtt ggccaacccg cgtggttttt gtgccggggt agaccgcgct
     8221 atcagcattg ttgaaaacgc gctggccatt tacggcgcac cgatatatgt ccgtcacgaa
     8281 gtggtacata accgctatgt ggtcgatagc ttgcgtgagc gtggggctat ctttattgag
     8341 cagattagcg aagtaccgga cggcgcgatc ctgattttct ccgcacacgg tgtttctcag
     8401 gcggtacgta acgaagcaaa aagccgcgat ttgacggtgt ttgatgccac ctgtccgctg
     8461 gtgaccaaag tgcatatgga agtcgcccgc gccagccgtc gtggcgaaga gtcgattctc
     8521 atcggccacg ccgggcatcc ggaagtggaa ggtacaatgg gccagtacag taacccggaa
     8581 gggggaatgt atctggtcga atcgccggac gatgtgtgga aactgacggt caaaaacgaa
     8641 gagaagctct cctttatgac ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc
     8701 gacgcgctgc gtaaacgctt cccgaaaatt gtcggtccgc gcaaagatga catctgctac
     8761 gccacgacta accgtcagga agcggtacgc gccctggcag aacaggcgga agttgtgttg
     8821 gtggtcggtt cgaaaaactc ctccaactcc aaccgtctgg cggagctggc ccagcgtatg
     8881 ggcaaacgcg cgtttttgat tgacgatgcg aaagatatcc aggaagagtg ggtgaaagag
     8941 gttaaatgcg tcggcgtgac tgcgggcgca tcggctccgg atattctggt gcaaaatgtg
     9001 gtggcacgtt tgcagcagct gggcggtggt gaagccattc cgctggaagg ccgtgaagaa
     9061 aacattgttt tcgaagtgcc gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc
     9121 attagcagcc taagttatgc gaaaatgccg gtcttgttac cggcattttt tatggagaaa
     9181 acatgcgttt acctatcttc ctcgatactg accccggcat tgacgatgcc gtcgccattg
     9241 ccgccgcgat ttttgccccc gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg
     9301 tctcggttga gaaaaccacc cgcaacgccc tgcaactgct gcatttctgg aatgcggaga
     9361 ttccgctcgc ccagggagcc gctgtgccgc tggtacgcgc accgcgtgat gcagcatctg
     9421 tgcacggcga atcgggaatg gctggctacg actttgttga gcacaaccga aagccaatcg
     9481 ggataccagc gtttctggcg attcgtgatg ccctgatgcg tgcaccagag cctgttaccc
     9541 tggtggccat tggcccgtta accaatattg cgctgttact ttcacaatgc ccggaatgta
     9601 aaccgtatat tcgtcgtctg gtgatcatgg gcggttcttc tggtcgcggc aactgtacac
     9661 caaatgccga gtttaatatc gctgccgatc cagaagcggc tgcctgtgtc ttccgtagcg
     9721 gaatcgaaat cgtcatgtgc ggtctggatg tcaccaatca ggcaatatta acccctgact
     9781 atctcgctac tttgccggag ttgaatcgca ccggaaaaat gcttcatgca ctgtttagcc
     9841 actaccgtag cggcagtatg caaagcggtc tgcgaatgca cgatctctgc gccatcgcct
     9901 ggctggtgcg cccggactta ttcaccctca agccgtgttt tgtcgcggta gaaactcaag
     9961 gcgaatttac ctcaggcacg acggtggttg atatcgacgg ttgcctgggc aaaccagcca
    10021 atgtaaaggt ggcattggat ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc
    10081 tggctctggc gtcgtaacct gtcacatgtt atcggcatgc agtcattcat cgactcatgc
    10141 ctttcactga tatccctccc tgtttatcat taatttctaa ttatcagcgt ttttggctgg
    10201 cggcgtagcg atgcgctggt tactctgaaa acggtctatg caaattaaca aaagagaata
    10261 gctatgcatg atgcaaacat ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc
    10321 cagttgattc aggcggcgct ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc
    10381 gaaggatctt ctttactggg cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc
    10441 gttaccgtgc aaagcagcct cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt
    10501 acccgtccgg aaggtacgct gaaccatctc gctttttgtc gccagcatgg caaagggatg
    10561 gtgatcggca ctacggggtt tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc
    10621 gatattgcga ttgtctttgc cgccaatttt agcgttggcg ttaacgtcat gcttaagctg
    10681 ctggagaaag cagccaaagt gatgggtgac tacaccgata tcgaaattat tgaagcacat
    10741 catagacata aagttgatgc gccgtcaggc accgcactgg caatgggaga ggcgatcgcc
    10801 cacgcccttg ataaagatct gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt
    10861 gaacgtgtgc ctggcaccat tggttttgcc accgtgcgtg caggtgacat cgttggtgaa
    10921 cataccgcga tgtttgccga tattggcgag cgtctggaga tcacccataa ggcgtccagc
    10981 cgtatgacat ttgctaacgg cgcggtaaga tcggctttgt ggttgagtgg taaggaaaat
    11041 ggtctttttg atatgcgaga tgtacttaat ctcaataatt tgtaaccaca aaatatttgt
    11101 tatggtgcaa aaataacaca tttaatttat tgattataaa gggctttaat ttttggccct
    11161 tttattttta gtgttatgtt tttaaatatt ctataagtgc caaaaattac atgttttgtc
    11221 ttctattttt gttgttttaa tgtaaatttt gaccatttgg tccacttttt cctgctcgtt
    11281 tttatttcat gcaatcttct tgctgcgcaa gcgttttcca gaacaggtta tatgatcttt
    11341 ttgtcgctta atgcctgtaa aatatgcatg agccacaaaa taatataaaa aataccgcca
    11401 ttaagttgac ttttagcgcc catatctcca gaatgccgcc gtttgccaga aattcgtcgg
    11461 taagcagatt tgcattgatt tacgtcatca ttgtgaatta atatgcaaat aaagtgagtg
    11521 aatattctct ggagggtgtt ttgattaagt cagcgctatt ggttctggaa gacggaaccc
    11581 agtttcacgg tcgggccata ggggcaacag gttcggcggt tggggaagtc gttttcaata
    11641 cttcaatgac cggttatcaa gaaatcctca ctgatccttc ctattctcgt caaatcgtta
    11701 ctcttactta tccccatatt ggcaatgtcg gcactaatga cgccgatgaa gaatcttctc
    11761 aggtacatgc acaaggtctg gtgattcgcg atctgccgct gattgccagc aacttccgta
    11821 ataccgaaga cctctcttct tacctgaagc gccataacat cgtggcgatt gccgatatcg
    11881 atacccgtaa gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg
    11941 caggcgataa cccggatgcg gcgttggcgt tagaaaaagc ccgcgcgttc ccaggtctga
    12001 atggcatgga tctggcaaaa gaagtgacca ccgcagaagc ctatagctgg acacaaggga
    12061 gctggacgtt gaccggcggc ctgccagaag cgaaaaaaga agacgagctg ccgttccacg
    12121 tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg gatagaggct
    12181 gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag
    12241 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg
    12301 ccatccagaa attcctcgaa accgatattc cggtattcgg catctgcctc ggtcatcagc
    12361 tgctggcgct ggcgagcggt gcgaagactg tcaaaatgaa atttggtcac cacggcggca
    12421 accatccggt taaagatgtg gagaaaaacg tggtgatgat caccgcccag aaccacggtt
    12481 ttgcggtgga tgaagcaaca ttacctgcaa acctgcgtgt cacgcataaa tccctgttcg
    12541 acggtacgtt acagggcatt catcgcactg ataaaccggc gttcagcttc cagggtcacc
    12601 cggaagccag ccctgggcca cacgacgcag cgccgttgtt cgaccacttt atcgagttaa
    12661 ttgagcagta ccgtaaaacc gctaagtaat caggagtaaa agagccatgc caaaacgtac
    12721 agatataaaa agtatcctga ttctgggtgc gggcccgatt gttatcggtc aggcgtgtga
    12781 gtttgactac tctggcgcgc aagcatgtaa agccctgcgc gaagagggtt accgcgtcat
    12841 tctggtgaac tccaacccgg cgaccatcat gaccgacccg gaaatggccg atgcgaccta
    12901 catcgagccg attcactggg aagtagtacg caagattatt gaaaaagagc gcccggacgc
    12961 ggtgctgcca acgatgggcg gtcagacggc gctgaactgc gcgctggagc tggagcgtca
    13021 gggcgtgttg gaagagttcg gcgtgactat gattggtgcg accgccgatg cgattgataa
    13081 agcagaagac cgccgtcgtt tcgacgtagc gatgaagaaa attggtctgg aaaccgcgcg
    13141 ttccggtatc gcacacacga tggaagaagc gctggcggtt gccgctgacg tgggcttccc
    13201 gtgcattatt cgcccatcct ttaccatggg cggtagcggc ggcggtatcg cttataaccg
    13261 cgaagagttt gaagaaattt gcgcccgcgg tctggatctc tccccaacca aagagctgct
    13321 gattgatgag tcgctgatcg gctggaaaga gtacgagatg gaagtggtgc gtgataaaaa
    13381 cgacaactgc atcatcgtct gctctatcga aaactttgat gcgatgggca tccataccgg
    13441 tgactccatc actgtcgcgc cagcccaaac gctgaccgac aaagaatatc aaatcatgcg
    13501 taacgcctcg atggcggtgc tgcgtgaaat cggcgttgaa accggtggtt ccaatgtcca
    13561 gtttgcggtg aacccgaaaa acggtcgcct gattgttatc gaaatgaacc cacgcgtgtc
    13621 ccgttcttcg gcgctggcgt cgaaagcgac cggtttcccg attgctaaag tggcggcgaa
    13681 actggcggtg ggttacaccc tcgacgaact gatgaacgac atcactggcg gacgtactcc
    13741 ggcctccttc gagccgtcca tcgactatgt ggttactaaa attcctcgct tcaacttcga
    13801 aaaattcgct ggtgctaacg accgtctgac cactcagatg aaatcggttg gcgaagtgat
    13861 ggcgattggt cgcacgcagc aggaatccct gcaaaaagcg ctgcgcggcc tggaagtcgg
    13921 tgcgactgga ttcgacccga aagtgagcct cgatgacccg gaagcgttaa ccaaaatccg
    13981 tcgcgaactg aaagatgctg gcgcagagcg tatctggtac atcgccgatg ccttccgtgc
    14041 gggcctgtct gtggacggcg tgttcaacct gaccaatatt gaccgctggt tcctggtaca
    14101 gattgaagaa ctggtgcgtc tggaagagaa agtagcggaa gtgggcatca ctggcctgaa
    14161 cgctgaattc ctgcgccagc tgaaacgcaa aggctttgcc gatgcgcgtc tggcaaaact
    14221 ggcgggcgtg cgcgaagcgg aaatccgcaa gctgcgcgac cagtatgacc tgcacccggt
    14281 ttataagcgc gtggatacct gtgcggcaga gttcgccacc gacaccgctt acatgtactc
    14341 cacttatgaa gaagagtgcg aagcgaatcc gtctaccgac cgtgaaaaaa tcatggttct
    14401 cggcggcggc ccgaaccgta tcggtcaggg tatcgaattc gactactgct gtgtacacgc
    14461 ctcgctggcg ctgcgcgaag acggttacga aaccattatg gttaactgta acccggaaac
    14521 cgtctccacc gactacgaca cttccgatcg cctctacttc gagccggtaa ctctggaaga
    14581 tgtgctggaa atcgtgcgta ttgagaagcc gaaaggcgtt atcgttcagt acggcggtca
    14641 gaccccgctg aaactggcgc gcgcgctgga agctgctggc gtaccggtta tcggcaccag
    14701 cccggatgct atcgaccgtg cagaagaccg tgaacgcttc cagcatgcgg ttgagcgtct
    14761 gaaactgaaa caaccggcga acgccaccgt taccgctatt gaaatggcgg tagagaaggc
    14821 gaaagagatt ggctacccac tggtggtacg tccgtcttac gttctcggcg gtcgggcgat
    14881 ggaaatcgtc tatgacgaag ctgacctgcg tcgctacttc cagacggcgg tcagcgtgtc
    14941 taacgatgcg ccagtgttgc tggatcactt ccttgatgac gcggtagaag ttgacgtgga
    15001 tgccatctgc gacggcgaaa tggtgctgat tggcggcatc atggagcaca tcgagcaggc
    15061 gggcgtgcac tccggtgact ccgcatgttc tctgccagcc tacaccttaa gtcaggaaat
    15121 tcaggatgtg atgcgccagc aggtgcagaa actggccttc gaattgcagg tgcgcggtct
    15181 gatgaacgtg cagtttgcgg tgaaaaacaa cgaagtctac ctgattgaag ttaacccgcg
    15241 tgcggcgcgt accgttccgt tcgtctccaa agccaccggc gtaccgctgg caaaagtggc
    15301 ggcgcgtgtg atggctggca aatcgctggc tgagcagggc gtaaccaaag aagttatccc
    15361 gccgtactac tcggtgaaag aagtggtgct gccgttcaat aaattcccgg gtgttgaccc
    15421 gctgttaggg ccagaaatgc gctccaccgg ggaagtgatg ggcgtgggcc gcaccttcgc
    15481 tgaagcgttt gccaaagcgc agctgggcag caactccacc atgaagaaac acggtcgtgc
    15541 gttgctttcc gtgcgcgaag gcgataaaga acgcgtggtg gacctggcgg caaaactgct
    15601 gaaacagggc ttcgagctgg atgcgaccca cggcacggcg attgtgctgg gcgaagcggg
    15661 cattaatccg cgtctggtga acaaggtgca tgaaggccgt ccgcacattc aggaccgcat
    15721 caagaatggc gaatatacct acatcatcaa caccacttca ggccgtcgcg cgattgaaga
    15781 ctcccgcgtg atccgtcgca gtgcgctgca atataaagtg cattatgaca ccaccctgaa
    15841 cggtggtttc gctaccgcga tggcgctgaa tgccgacgcg accgaaaaag taatttcggt
    15901 gcaggaaatg cacgcgcaga tcaaataata gcgtgtcatg gcggatattt ttcatccgct
    15961 aatttgatcg aataagtaat acggttctct gatgaggacc gttttttttg cccattaatt
    16021 aaatcttttg gggaatcgat atttttgatg acataagcag gatttagctc acact
//
LOCUS       DAJVXP010000095        15963 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVXP010000095 DAJVXP010000000
VERSION     DAJVXP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.095"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..455)
                     /gene="spy"
                     /locus_tag="OQS10_002855"
     CDS             complement(<1..455)
                     /gene="spy"
                     /locus_tag="OQS10_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS2293833.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAA"
     gene            complement(785..1753)
                     /gene="astE"
                     /locus_tag="OQS10_002856"
     CDS             complement(785..1753)
                     /gene="astE"
                     /locus_tag="OQS10_002856"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS2293834.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1746..3089)
                     /gene="astB"
                     /locus_tag="OQS10_002857"
     CDS             complement(1746..3089)
                     /gene="astB"
                     /locus_tag="OQS10_002857"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS2293835.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3086..4564)
                     /gene="astD"
                     /locus_tag="OQS10_002858"
                     /pseudo
     CDS             complement(3086..4564)
                     /gene="astD"
                     /locus_tag="OQS10_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4561..5595)
                     /gene="astA"
                     /locus_tag="OQS10_002859"
     CDS             complement(4561..5595)
                     /gene="astA"
                     /locus_tag="OQS10_002859"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS2293836.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5592..6812)
                     /gene="astC"
                     /locus_tag="OQS10_002860"
     CDS             complement(5592..6812)
                     /gene="astC"
                     /locus_tag="OQS10_002860"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCS2293837.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7258..8064
                     /gene="xthA"
                     /locus_tag="OQS10_002861"
     CDS             7258..8064
                     /gene="xthA"
                     /locus_tag="OQS10_002861"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS2293838.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8231..8941
                     /locus_tag="OQS10_002862"
     CDS             8231..8941
                     /locus_tag="OQS10_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS2293839.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8946..9623
                     /gene="ydjY"
                     /locus_tag="OQS10_002863"
     CDS             8946..9623
                     /gene="ydjY"
                     /locus_tag="OQS10_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS2293840.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9638..10345
                     /locus_tag="OQS10_002864"
     CDS             9638..10345
                     /locus_tag="OQS10_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS2293841.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10345..10893
                     /locus_tag="OQS10_002865"
     CDS             10345..10893
                     /locus_tag="OQS10_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS2293842.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10903..12069
                     /locus_tag="OQS10_002866"
     CDS             10903..12069
                     /locus_tag="OQS10_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2293843.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12042..13578
                     /locus_tag="OQS10_002867"
                     /pseudo
     CDS             12042..13578
                     /locus_tag="OQS10_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13578..14231
                     /locus_tag="OQS10_002868"
     CDS             13578..14231
                     /locus_tag="OQS10_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS2293844.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14298..15605
                     /gene="ynjE"
                     /locus_tag="OQS10_002869"
     CDS             14298..15605
                     /gene="ynjE"
                     /locus_tag="OQS10_002869"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS2293845.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15614..>15963)
                     /locus_tag="OQS10_002870"
     CDS             complement(15614..>15963)
                     /locus_tag="OQS10_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS2293846.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3701 a         4346 c         4162 g         3754 t
ORIGIN      
        1 gccgctggac gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc
       61 ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg
      121 cgctgttctt ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg
      181 gcaatgatgt catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc
      241 tggcctttca tgatttcgcg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg
      301 aacatcatgt cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca
      361 gccggtgctg cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg
      421 gtagaggcaa caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat
      481 ttattacggc tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc
      541 gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt
      601 atggagaaaa aaagaaaatt tgatggagag tgatgagaga atattacaac acaatgattt
      661 tgcagagatt atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga
      721 ttaatctgta ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta
      781 agtattagct tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat
      841 tagggaataa cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg
      901 ccagcaatgt tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt
      961 cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc
     1021 tcacgatacc gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa
     1081 actggcgaag atcgttttgc ccaaagggca acgctttgcc aagttccagc gtacaggcca
     1141 gcgcgccaaa atgtctggcg ctgaaatggg taaacgtacc accaggctcc tgatggaaca
     1201 ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt
     1261 cgcgttgcgg taatacaccg aactgaggat gcaaggagcc acgaattgcg gtatgtagat
     1321 caaggtgcca acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca
     1381 gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca
     1441 ttcggttcat atcgctatgg caataacgtt tcccttgctt cagcgcagga ggatccccga
     1501 ggatcaccag caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg
     1561 cgtccaacat ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg
     1621 cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc
     1681 gaacgccgtt gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa
     1741 gaaaattatc cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt
     1801 aattggctca atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga
     1861 tcggcagcag taaggcgatc gcggtagtaa cgatccaccc agtcattgag cgcattaaac
     1921 agcgtatcgt tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatact
     1981 acccgcaacc gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taaatcaaag
     2041 acttttagtt cgctaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact
     2101 ccggcgtgtt cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc
     2161 aacagttggc tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc
     2221 ggaacttcta tcgccataaa accgttcacc cgcgcccgca ggtttgccag taactgtgac
     2281 tggcgagcga acgcctgttg gtggcaaaac agcacctggc ggttacttac ggcaatcacg
     2341 tcattatgaa aaacaccctg gtcgataacg tccgggtttt gctgggcgaa aatcacctgt
     2401 tggggattca cctgattaag ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc
     2461 ggataacggg aaggccgggt atcattgcct tcttctcgcc cgtagacaaa aagttgcata
     2521 cccggctcac cgtaatgacc gccgagacga ttgtggtttg ccgccccctc atcaccgagc
     2581 aacgctacct gcggcaacgc cgaatggacg ctaaatttct cttcgtcgtt aaaaatagct
     2641 tttaacagcg attcagtgac gggcgcttcc agcgaacggt gaaatttatt gttcaggttg
     2701 gcaacggtga gatgcacttt accatccagc gtatcggcag atggcgcgat cgttgccgca
     2761 ttggctaccc acattggcga agcggagctg acgctggaaa gccagtgcgg tgcctggcgt
     2821 gcaacttttt ccagtacctg ctcatcgcta ccactgaatc ccaactgacg cagcaccgga
     2881 ataaacggac gctcgtgcgg cgggatcacg gcctggggga atcccgcatc ggcaagggtt
     2941 ttcattttca gtaagccctg tttcgccgcc agtcgcgggt tagacacctg aaaacggtga
     3001 cgggtagagg cttcattacc aaacgacagg ccagcgtaat gatgcgtcag ccctaccagc
     3061 ccgtcgaaat taacttccca ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg
     3121 ggttaagcgt tgcgggcaaa gttaacgagt ccgactccag gctcgccata ggccatgcgc
     3181 agtaatctgc ggcataccag gcgctggggc gatggttacc ggatgcacca atgccgccga
     3241 atggcgcggt actggcagca ccagtaagcg gtttgttcca gttaacaatc cccacccgcg
     3301 cctccagcaa cagttgatcg aatttttccc gctcggggga aaccagaccg caagagagtc
     3361 cgaagcgagt gttattcgcc attagaatcg cttcctcgaa agaatcataa cgccagacgc
     3421 gcaataacgg tccgaacacc tcttcatctg gtacgccagc aacgcctgtc atttcaatga
     3481 tccccggcgt cagcaacgat gtctctgatt gtaataagcg cggcgcaagc agggttcgtc
     3541 cgcccatcgc ttccagttgc tgctaggcag taaccacctg ctgtgcggcc tgttcagaaa
     3601 tcagcccgcc aataaacggc tgcggttcgt catcccagtt gcccggcgtt aatcgctggc
     3661 tgacggcaac cagacgagca agaaacgcat cgccctgtgc tccgcttttg agcaataaac
     3721 ggcgggcgca ggtgcagcgt tgcccggctg tgacaaacgc cgactgaatg gtcagatgga
     3781 cagccgagtc gatatccgcc acctcatcga taattagcgg attattaccg cccatctcaa
     3841 gggcgagaat tttctccggc tgaccggaga gctggcgatg cagctggtag ccggtattgg
     3901 cgctaccggt aaacagcaaa ccgtcgagat cctccagcgc actcagcgcc tgacccgttt
     3961 cacgcccgcc ctgcaccagg ttcagcacgc ccggcggcaa gccagcctgc tgccataaac
     4021 gcattaccgc ttcgccactc cacggtgtca gttcgctggg tttaaagata atggtgttac
     4081 ctgccagcaa tgccggaacg atatgtccgt tcggcaaatg accagggaaa ttatacggcc
     4141 caaacaccgc cagcacgccg tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt
     4201 cactacgctg ctcgccggta cgaacgtgat acgccttaat tgatatcgcg attttattga
     4261 tcatcgccgt cacttcggtt gccgcttccc agcgcggctt acccgtttct ctggcaataa
     4321 tcgcggttaa ttcggcttta ttgctttcca gcaatccggc aaagcgttcg acaacgacct
     4381 gacgttcagc caatgagagc cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag
     4441 cctgttcgac ctgagcggca tcggcatcat tgccttgcca taacacctcg cccgataccg
     4501 gattacgctt cacacgcgat gcgccctggc ccgttatcca gtcaccgtta atccataaag
     4561 tcatgctgtt ttctcctctg cgcacaggcg caccagacga acgcgatccc ctgcgtggca
     4621 tttgagggca tccagttgtg cggcggttaa aatcaaacgc tcggttgccg gatcggtacg
     4681 cgccagcacc acgcggaaat ggtgataatt ttcattggcg accaggcagg ccggaaaatc
     4741 gccctgcgca ggctgccctt ctgccacttc caccagccga cttttacgga tggcgcgcac
     4801 gcggtcgatg tcacactcaa gcgttggccc gccgtcgaag atgtcgatat agttgcggta
     4861 acgaaaacct tctttctcca gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc
     4921 gatgacgtcc tgggcttcct gggataaaaa gtgggtatag atcggatgtt tcggcatcag
     4981 ttctgcaata aatgcctttt gcccggtgcc gcagagaaaa tcgacgcggc taaaatccat
     5041 cgaaaagaag cgtttaccga ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac
     5101 cccgcgcatt tcagcaacca ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat
     5161 aaagcgcgat ttcgacagca aatagccgtt gccctctttg cgccagtccg ggtcgagaaa
     5221 cagcgtgcac agctcgctgc tgccggtgtg atcgttacta agaaacagcg tcggcaatgc
     5281 gttatagaca ttcagctctt ttgaggcgtg aaccaacgtg ccgacccgat agttgtacca
     5341 gggatcgttc agcccaaccg ccacctcaat ggcgcaaatc cccgccacgg tgcctgtctc
     5401 gctatcttcc agcacgaaca catagccctg ctcgcttttg ggcagttcgc cttgccaggt
     5461 tttgattgcc ctttcgatac gcgcggaaag cgtggcttca ttggcgggaa gcgacgtcag
     5521 gccgccgccc gttttgctgg caagctgcat cagcgccgag acatctgagc gctcaacggg
     5581 acggatgacc atcatgatga acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga
     5641 tccagtccgg tcgtcacctc ttcttcgctg acattgagcg caggcgcaaa acgcaccacg
     5701 ttgccacccg caatcagtac cataacgcct gctttcgccg cctcctgaga gatctgtttc
     5761 gcttgtccgg cgtaatcggc attcagtaca cagccaatca acaaacctaa gccgcgaact
     5821 tcactgaaca aaccgtagcg gtgattaatg gtattaagac gctcaacaaa ccagtcgtga
     5881 cgctgtttaa cgccattaag catctctggt gtgttgatga gctccagcac tttgcccgcc
     5941 accgccgagg ccagcggatt gccgccatag gtggtgccat gagtgccaac ggtcatcacg
     6001 ctggcgcact cttcggttgt taacaacgca ccgaccggga aaccgccgcc cagcgctttg
     6061 gcggtagtta acagatcagg tgtcacgccg tagtgcatat aggcatacag ttccccggtg
     6121 cgcccgacgc cggtttgtac ttcatcaaaa atcaacagcg cattgtggcg gtcacacaat
     6181 tcacgcagac cttgtaaaaa cgcgttgctg gctggcacca caccgccttc cccctggatg
     6241 ggttcgacaa tcaccgcaca ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg
     6301 ttatatgcag catgacggat atctggtggc agtggcgcaa aatcctgtga ataggctggc
     6361 tgcccacccg cactgacagt aaacagcgtg cgaccatgaa acgcattttt aaacgccacg
     6421 ataccgctct tatggctgcc gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc
     6481 gcttcgttgg cttccgcacc ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg
     6541 atcaattttt tcgccagtcg cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag
     6601 aacttactcg cctgttcgtt cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg
     6661 ttcaccgcaa tgccacccgc gaagtcgata tactctttcc cctgctgatc ccacaagcgc
     6721 gaaccttcgc cacgtaccgg tataaagggt gccggagcgt aaacaggtat catccattca
     6781 tcaaagtttt cacgcgtaat tggctgagac atagcgacct ctacagtaaa taattcgcta
     6841 tctatatgtt aacaataagt aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca
     6901 gccagtgata aaagtgcata aacggcggag gctaactgga aatcaaggag ttataaccaa
     6961 accatatgca tttaaagtgc atataaagtg aatacgtttg cgatgtgggt gaataaaaag
     7021 aataaaaaac gcaatgttat gcagaagtaa aatataattc tggaattgtg atcattgacg
     7081 aaatttactg gaaattactg cgccattctg acgcagcgcg caccaaaagc gggcattttt
     7141 tgcgccatcg ttgacatcat taacaaccat cgatcaaatc acttaacaac aggcggtaag
     7201 caacgcgaaa ttctgctacc atccacgcac tctttatctg aataaatggc agcgactatg
     7261 aaatttgtct cttttaatat caacggcctg cgcgccagac ctcaccagct tgaagccatc
     7321 gtcgaaaagc accaaccgga tgtgattggc ctgcaggaga caaaagttca tgacgatatg
     7381 tttccgctcg aagaggtggc gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc
     7441 cattatggcg tggcgctgct gaccaaagag acgccgattg ccgtgcgtcg cggctttccc
     7501 ggtgacgacg aagaggcgca gcggcggatt attatggcgg aaatcccctc actgctgggt
     7561 aatgtcaccg tgatcaacgg ttacttcccg cagggtgaaa gccgcgacca tccgataaaa
     7621 ttcccggcaa aagcgcagtt ttatcagaat ctgcaaaact acctggaaac cgaactcaaa
     7681 cgtgataatc cggtactgat tatgggcgat atgaatatca gccctacaga tctggatatc
     7741 ggcattggcg aagaaaaccg taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg
     7801 gaagagcgcg aatggatgga caggctgatg agctgggggt tggtcgatac cttccgccat
     7861 gctaatccgc aaacagcaga tcgtttctca tggtttgatt accgctcaaa aggttttgac
     7921 gataaccgtg gtctgcgcat cgacctgctg ctcgccagcc aaccgctggc agaatgttgc
     7981 gtagaaaccg gcatcgacta tgaaattcgc agcatggaaa aaccgtccga tcacgccccc
     8041 gtctgggcga ccttccgccg ctaatttagc agctctcctg gctcaaactg ggtcaggaga
     8101 attaaccttg agaaaaatca acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt
     8161 tataccgtca ctgcgttttt agttgtctga ccacttctct attatcaagt ttgatatagg
     8221 aaactccacg atgaatgctg agcgtaaatt tcttttcgcc tgtcttattt ttgcgctggt
     8281 catttacgct atccacgctt tcggtttatt cgatctgctc accgatttac cccacttaca
     8341 gacactcatt cgccagagcg gattgttcgg ttatagcctc tatattctgt tattcatcat
     8401 tgccaccctc tttgtgttac caggaagcat actggtgatc gctggaggaa tagtttttgg
     8461 cccgctctta gggacactac tctcattaat tgccgccacg ctggcctcct cgtgctcatt
     8521 cctgctggcg cggtggctgg gacgtgattt actgctgaaa tacgtgggcc atagccatac
     8581 cttccaggcc attgaaaaag gcattgcgcg taacggtatt gattttctta ttctgacccg
     8641 cttaatcccg ttgtttcctt acaatattca aaattacgct tacggattaa ccaccatcgc
     8701 cttctggcct tataccctta tttcggcact cacgaccctg cccggtattg ttatttatac
     8761 cgtgatggca agcgatctcg ccaatgaagg cattacgctg cgctttattt tacaactctg
     8821 tctggcgggc ctggcgctgt ttattctcgt ccagctcgca aaactctacg cccgacacaa
     8881 acatgtggat ctgtctgctt cgcgccgcag cccgcttact cacccaaaaa atgaaggata
     8941 gaacgatgtc gcaacattat tcagtgtcat ggaaaaaagg actggctgca ctctgcttac
     9001 tggctgttgc cgggcttagc ggctgcgatc aaaaagagaa tgcggcggca aaagtggaat
     9061 acgacggact ttcgaacagc caaccgctgc gcgtcgatgc caataaccat acggtaacca
     9121 tgttggtgca aattaatggt cgtttcctca ccgacgacac tcgtcacggt attgtgttta
     9181 aagatggctc caacggacat aaatcgctgt ttatggctta tgcgaccccg aaagcatttt
     9241 atgaagccct gaaagaggca ggtggtacgc cgggcgaaaa catgacgatg gataataaag
     9301 aaacgactca tgtcacaggc agcaaactgg atatttcggt caactggcaa ggggcggcaa
     9361 aagcgtattc cttcgatgaa gtgattgttg acagtaatgg caagaaactg gacatgcgct
     9421 ttggcggtaa tttaacggca gcagaagaga agaaaacggg ttgcctggtg tgtctggata
     9481 gctgcccggt cggcatcgtc agcaatgcaa catacactta tggtgcggtg gaaaaacgcg
     9541 gcgaagtgaa attcaaaggc aatgcctcag ttctcccggc ggataacacg ctggcaacgg
     9601 ttacctttaa aatcaccgaa taaagccagg ataaaggatg atgaagatgc aatcgcgtaa
     9661 aatctggtac taccgtataa ccctcatcat cctgttgttc gctatgctgc tcgcatgggc
     9721 gctgcttccc ggcgtccatg agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga
     9781 ccaacagggt atagaacgct ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt
     9841 cttgttgatg attttacagg ccattgctgc accgctacct gcgtttttga tcacctttgc
     9901 caatgcgtcg ctgtttggcg cgttctgggg cggcttgctg tcgtggacca gttcgatggc
     9961 cggcgcggcg ctgtgctttt ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt
    10021 aaccggcaaa accgtgcttg acagtatgga cggctttttc actcgctacg gcaaacacac
    10081 catcctggtc tgtcggttat tgccttttgt ccctttcgat ccaatcagct atgctgccga
    10141 tctgacttca atacgttttc gctcgttttt tatcgccacc gggcttggtc aattaccgac
    10201 gactattgtt tattcctggg cgggcagcat gttaacaggc ggtactttct ggtttgtcac
    10261 cggactgttt attctgtttg ccctgaccgt ggtgattttt atggcgaaga aaatatggct
    10321 tgagcgccag aagaggaatg actgatgggt ttaccgccgc ttagcaaaat tcctttcatt
    10381 ttacgtccgc aggcgtggct gcatcgtcgc cattacggcg aggtgctaag cccgattcgc
    10441 tggtgggggc ggatcccgtt tatcttttat ttggtgtcaa tgtttgttgg gtggctggag
    10501 cgcaaacgct caccgctcga tccggtagtg cgatcgcttg tcagcgcgcg cattgcgcaa
    10561 atgtgcctgt gtgagttttg tgtggatatc accagtatga aagtcgccga gcgtaccggc
    10621 agcagcgata aactgctggc agtggctgac tggcggcaaa acccgctctt tagcgatgaa
    10681 gaacggctgg cgctggagta cgccgaagcc gcaagcgtca caccgccaac agtcgatgat
    10741 gccctgcgta cccgactggc tgcgcatttt gacgctcagg cgctcaccga actgacggca
    10801 ttgatcggcc tgcaaaacct gtcggctcgt tttaattctg ccatggacat tcccgctcag
    10861 gggctgtgcc gtattccgga aaaacgttct taaggagaga agatgcgcca ttgtgggtgg
    10921 ttgctgggat tgttatcgct gttttctttg gcaacacatg ccagtgactg gcaggaaatt
    10981 aaaaatgagg ccaaagggca aaccgtctgg tttaacgcct ggggcggcga taccgcaatt
    11041 aaccgctatc tcgactgggt tagcggcgag atgaaaaccc attacgctat aaacctgaag
    11101 attgttcgtc tcgcggatgc cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc
    11161 ggtcgtaaaa cgggcggctc ggtggatctg ctctgggtga acggcgaaaa cttccgcacc
    11221 ttaaaagagg ccaatttatt acaaacgggc tgggcggaga ctctgcccaa ctggcgctat
    11281 gtcgacacac agctgccggt gcgggaagat ttttcagtgc cgacacaagg tgcggaatcg
    11341 ccctggggcg gcgcacaact gacgtttatc gcccgccgcg atgttacgcc acagccacca
    11401 caaacgccgc aagccttact ggagtttgct aaagccaatc ccggcacggt tacttatccg
    11461 cgcccaccgg actttaccgg cacggcgttt cttgaacagt tgctgattat gctgacgcca
    11521 gatcccgccg cattaaaaga agcgccggac gatgcgactt tcgcccgtgt cactgcgccc
    11581 ttgtggcaat atcttgatgc actgcatccg tatttgtggc gcgaaggaaa ggatttcccg
    11641 ccttcgcccg cgcggatgga tgctctgctg aaagccggca cattgcgcct gtcgctgacc
    11701 tttaaccccg cgcatgcgca gcaaaaaatc gccagcggcg atttgccggc aagcagttac
    11761 agttttggct ttcgcgaggg gatgattggc aacgtgcatt tcgtcaccat tccagccaac
    11821 gcgaatgcca gtgctgcggc gaaggtagtt gccaatttcc tgctctcacc cgatgcgcaa
    11881 ctgcgtaaag cagatcccgt tgtctggggc gatccttctg ttctcgatcc gcaaaaactg
    11941 cctgacgggc agcgcgaaat attgcaatca agaatgccgc aggatctgcc gccggtactg
    12001 gctgaaccgc acgcaggatg ggtgaatgca ctggaacaag aatggttacg ccgttacggt
    12061 acgcattaat ctttttgctg tgggcgatgg tggcggtgat ttatgcaccg ctgatcccgg
    12121 cagctctcac gctgatatcg cctgcgttgt cgttgacaca ctggcaggcg ttatttgccg
    12181 atccacagtt accgcatgca ttactggcaa cgctggtatc gacaaccatc gcggcggtcg
    12241 gggcattgtt gattgccctg ctggtgattg tggcgctata gcctgggccg aaatggcagc
    12301 gtatgtgcgc ccgtctgccg tggctgctcg ccattcccca tgtggctttt gccaccagcg
    12361 ccctgctgct ctttgctgac ggagggctgc tatatgacta tttcccgtat ttcactccgc
    12421 caatggaccg atttggcatc ggtctgggcc tcacccttgc agtgaaagaa agcgcatttc
    12481 tgctgtggat cttagcggca gtattgagcg aaaaacggct gttgcagcag gtcattgtgc
    12541 tggattcgct gggctacagc cgctggcaat gcctgaactg gctgctgttg ccctccgtcg
    12601 cgccttcgct ggcaatggcg atgctggcga ttgttgcctg gtcgctttcg gtcgtggatg
    12661 tggcgattat tcttgggcca ggtaatccac cgacgctggc agtaattagc tggcagtggt
    12721 taactcaggg cgacgcggat caacaaacga aaggcgcact cgccagtctg ctgctgatgc
    12781 tgttactcgc cgcctacgtt ttgctgagct atctgctatg gcgcagctgg cggcgcacta
    12841 ttccccgcgt agatggcatt cgcaatgcct gccacacctt tattaccagg cactacgctg
    12901 gcgagttttt tacccttaac cggtgtgctg tgtgtggttc tgctggcgat cctcgcggat
    12961 cagtcgacga tcaatagtga agcactcatc aacagtctga caatggggct ggtagcaaca
    13021 ttcatcgctt tgctcctgtt actgctgtgg ctggaatggg ggccacagcg tcgccagttg
    13081 tggctatggt tgcccattct attgcccgat ctgccactgg tggcgggcca gtacacgctg
    13141 gcgctatggc tgaacctgga tggaagctgg acggcggtgg tctgggggca tctgctgtgg
    13201 gtaatgccgt ggatgctgtt tatcctgcaa cccgcctggc agcgcattga ttcacggtta
    13261 attttgattg cgcaaacact gggctggtcg cgggccaaaa tcttctttta cgtgaaatgc
    13321 ccactcatgt tgcgccctgt gctgattgcc ttcgcggtgg gattttcagt cagtattgcg
    13381 cagtatatgc cgacgctgtg gctgggcgcg gggcgttttc cgacgctcac cactgaggcg
    13441 gtggcattaa gcagcggcgg cagcaacggt attctcgccg cccaggcttt atggcaactg
    13501 ttgttaccgc ttattatttt tgccctgacc gcgttagtcg caaaatgggt aggttatgtc
    13561 agacaaggac tccgctaatg ctctgcgtga aaaatgtttc gctacgttta ccagaaagcc
    13621 gcttgctgac aaacgttaac tttacggttg ataaaggtga catcgtcacg ttaatggggc
    13681 cgtctggctg tggaaaatcc actctgtttt catggatgat tggtgcactg gctggacagt
    13741 tttcttgtac aggtgagcta tggctcaatg agcaacggat tgacatgcta cccaccgcac
    13801 agcgtcagat tggcattctt ttccaggatg cactgttatt tgaccagttc agcgtcgggc
    13861 aaaatttact gctggcgcta ctgtcgacgc tcaaggggac tgccaggcgc aatgccgtga
    13921 aagacgcgct tgatcgagcg ggactggctg aaacttatca ccaggaccct gcgacattat
    13981 caggcggtca gcgagcgcgg gtggccctgc tgcgcgccct tctcgctcag ccaaaagcgt
    14041 tactcctgga tgagccattc agccgtcttg atgtggctct gcgcgataat tttcgccagt
    14101 gggtgttcag cgaagttcgc gaactggcga tccccgtcgt tcaggtaacg cacgatctcc
    14161 aggatgttcc tgctgatagt tctgttctgg atatggcgca gtggtcagaa aattacaaca
    14221 aactgcgata acgcaaagtt tttctcaatg cgtcagttca gaatggcgca ctcaaaacta
    14281 caatgtcggg attttcgatg aaacgtgttt ctcaaatgac cgcgctggca atggctttag
    14341 ggctggcttg cgcttcttcg tgggccgctg aactggcgaa gcctcttaca cttgaccagc
    14401 ttcaacaaca aaatggcaaa gcgatagata ctcgccccag cgcgttttat aacggctggc
    14461 cacaaacctt aaatggccct tctggtcatg aacctgccgc cttaaacctc tctgccagct
    14521 ggctcgacaa aatgagcacc gaacagctca acgcgtggat caagcaacat aacctgaaag
    14581 ccgatgcccc ggtggcgctg tacggtaatg acaaagatgt cgacgccgtc aaaacgcgac
    14641 tgcaaaaagc aggttttacg catatctcca ttctgagtga cgcgctaagc gaaccttccc
    14701 gtctacaaaa actgccgcac tttgagcagc tggtttatcc gcaatggctg cacgacctgc
    14761 aacaaggtaa agaggttacg gcgaaacctg ccggtgactg gaaagtcatt gaagcggcct
    14821 ggggcgctcc taagctttac cttatcagcc atattcccgg tgctgactac atcgatacca
    14881 acgaagtgga aagcgaaccg ctgtggaaca aagtttctga tgaacaacta aaagcgatgc
    14941 tggcaaaaca cggcattcgc catgacacca cggtcattct gtacgggcgt gacgtatacg
    15001 ctggagcgcg tgtggcgcaa attatgctct acgcaggcgt gaaagatgtg cgtctgctgg
    15061 atggcggctg gcaaacctgg tccgacgcgg gactgcctgt tgagcgagga acgccaccga
    15121 aagtgaaagc ggaaccggat ttcggcgtga agctcccggc acaaccgcag ttgatgctcg
    15181 atatggaaca ggcccgtgga ctgctgcatc gccaggatgc atcgctggtg agcattcgtt
    15241 cgtggccaga atttatcggt acgaccagcg gttacagcta tattaaacca aaaggtgaaa
    15301 tagccggagc acgttgggga cacgctggta gcgactcgac gcatatggaa gattttcata
    15361 acccggatgg caccatgcgc agcgccgatg atattaccgc tatgtggaaa gcgtggaata
    15421 tcaaaccaga gcagcaagtt tcattctact gcggcaccgg ctggcgcgcg tccgaaacct
    15481 ttatgtacgc acgcgccatg ggttggaaga atgtttccgt gtatgacggc ggctggtacg
    15541 aatggagcag cgatccaaaa aatccggtag caaccggtga acgcggcccg gacagtagca
    15601 aataacattg caattactga cgctggagcg acttcagcgt cagataccca ctccacaccc
    15661 gcgtaaaggt cgtcatccag cacaacgcac cgaagatcca cgcaaaccac gcaaaccatg
    15721 cagggaataa acatcccagc acaaacagta agatcgtttc tgtgccttcg gttaatcccc
    15781 ccagatagta aaacgactta tgtgcataac cggggttatc aatctgatgt tttgctgcca
    15841 gtgcagcaaa ggcgagaaaa ctgctgcccg tgccgataaa cgcaaacaac aaccagccgc
    15901 ctgccagcgc attttgctcc ggtgcagcaa gaataaagcc aaacggcacc agcgcgtaaa
    15961 aga
//
LOCUS       DAJVXP010000096        15745 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVXP010000096 DAJVXP010000000
VERSION     DAJVXP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.096"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..1453
                     /locus_tag="OQS10_002871"
                     /pseudo
     CDS             1..1453
                     /locus_tag="OQS10_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OQS10_002872"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OQS10_002872"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS2293847.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OQS10_002873"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OQS10_002873"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS2293848.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OQS10_002874"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OQS10_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS2293849.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="OQS10_002875"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="OQS10_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS2293850.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="OQS10_002876"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="OQS10_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS2293851.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OQS10_002877"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OQS10_002877"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS2293852.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OQS10_002878"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OQS10_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS2293853.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OQS10_002879"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OQS10_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS2293854.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="OQS10_002880"
     CDS             8572..9174
                     /locus_tag="OQS10_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS2293855.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OQS10_002881"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OQS10_002881"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS2293856.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="OQS10_002882"
     CDS             complement(9732..10472)
                     /locus_tag="OQS10_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2293857.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OQS10_002883"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OQS10_002883"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS2293858.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OQS10_002884"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OQS10_002884"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS2293859.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="OQS10_002885"
     CDS             13153..13719
                     /locus_tag="OQS10_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS2293860.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="OQS10_002886"
     CDS             14074..14322
                     /locus_tag="OQS10_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS2293861.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15745
                     /locus_tag="OQS10_002887"
     CDS             14923..>15745
                     /locus_tag="OQS10_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2293862.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT         3996 a         3995 c         3612 g         4142 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc
    15721 tcttcgccgg actaccacgg cccgc
//
LOCUS       DAJVXP010000097        15582 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVXP010000097 DAJVXP010000000
VERSION     DAJVXP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.097"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(4..333)
                     /locus_tag="OQS10_002888"
     CDS             complement(4..333)
                     /locus_tag="OQS10_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS2293863.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(338..523)
                     /locus_tag="OQS10_002889"
     CDS             complement(338..523)
                     /locus_tag="OQS10_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS2293864.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(520..3159)
                     /locus_tag="OQS10_002890"
     CDS             complement(520..3159)
                     /locus_tag="OQS10_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS2293865.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3367..4356
                     /gene="ldhA"
                     /locus_tag="OQS10_002891"
     CDS             3367..4356
                     /gene="ldhA"
                     /locus_tag="OQS10_002891"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS2293866.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4467..4889
                     /gene="hslJ"
                     /locus_tag="OQS10_002892"
     CDS             4467..4889
                     /gene="hslJ"
                     /locus_tag="OQS10_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS2293867.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4886..5152)
                     /locus_tag="OQS10_002893"
     CDS             complement(4886..5152)
                     /locus_tag="OQS10_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS2293868.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5426..8950
                     /gene="nifJ"
                     /locus_tag="OQS10_002894"
     CDS             5426..8950
                     /gene="nifJ"
                     /locus_tag="OQS10_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS2293869.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9316..10449
                     /gene="ompN"
                     /locus_tag="OQS10_002895"
     CDS             9316..10449
                     /gene="ompN"
                     /locus_tag="OQS10_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCS2293870.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10590..11024
                     /gene="uspF"
                     /locus_tag="OQS10_002896"
     CDS             10590..11024
                     /gene="uspF"
                     /locus_tag="OQS10_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS2293871.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11610..12524
                     /gene="sitA"
                     /locus_tag="OQS10_002897"
     CDS             11610..12524
                     /gene="sitA"
                     /locus_tag="OQS10_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS2293872.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12524..13351
                     /gene="sitB"
                     /locus_tag="OQS10_002898"
     CDS             12524..13351
                     /gene="sitB"
                     /locus_tag="OQS10_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS2293873.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13348..14205
                     /gene="sitC"
                     /locus_tag="OQS10_002899"
     CDS             13348..14205
                     /gene="sitC"
                     /locus_tag="OQS10_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS2293874.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14202..15059
                     /gene="sitD"
                     /locus_tag="OQS10_002900"
     CDS             14202..15059
                     /gene="sitD"
                     /locus_tag="OQS10_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS2293875.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15260..>15582)
                     /locus_tag="OQS10_002901"
     CDS             complement(15260..>15582)
                     /locus_tag="OQS10_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS2293876.1"
                     /translation="WRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDV
                     MTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNSVRN
                     FIVE"
BASE COUNT         3897 a         3953 c         3752 g         3980 t
ORIGIN      
        1 ctattaaaat tttcgcaccc atttaccgtt tatcccttgt acatattccc ccggtttggc
       61 ccgagctacg agcttttgcc cggcgagttt cgcgatatca tctaccgaca cattattttg
      121 ctttgccagt tgttggtaac tcgctttacg ttcggcatta atgtcggtta ctaatttctc
      181 tgtttcagca tccgttttca gcgcaaccag ataaccataa aatgtttcac ctacccgccc
      241 ctgagttctg gcttcatcca gagtcaatgc cattacattg ctgcttacca gcccaacaag
      301 aaacgcacaa agaagtaatg ttttcttcat catcgcctca gaaaagatcg ctacgagttt
      361 caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca attttaacgt
      421 tcatattgat agtgatcggt tccttgggcg cagcgacttc aatacgcggc gtacagccaa
      481 ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt cctcacactc
      541 ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc atgcctggag
      601 attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat gatggtaatt
      661 aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt tggcctgcat
      721 ggttaataaa ccgagattat ctaagttaat ttttgtcgat gaccggctaa tttccatata
      781 gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg catctgcggt
      841 gtctttatcc agccgcaagg tcattggccc gctatttgcc agccaaccat ctttcactat
      901 ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg acatggcgaa
      961 ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca gccgcaacag
     1021 cgccgggtca tgttgcggca tacgtaattg tttcatcagt acgttaccgc ctaacacatc
     1081 gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac gcccttgcaa
     1141 atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc gcaacgtaac
     1201 ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca ggacaaaatc
     1261 gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca cgccgtgtcc
     1321 tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat acagttcccc
     1381 atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag tcagtgattg
     1441 ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat ttacccgaac
     1501 cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg aaatacgtcg gatcgcgccc
     1561 atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga acagcgtttg
     1621 cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac gtacccagac
     1681 gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt attgtaactg
     1741 gtcgaaaccg gtggagaggt cggtcagcgt aatcgtgctg tcatgccatt caccttttcc
     1801 tgcgacatcc caggtggcat ttatcggtgt aaaactgcct tttccccagt agcgccactg
     1861 ccagcggccc gcgtcaggga gaaaatcatt cgccagccca tccatatgca gcacgaaatc
     1921 gcccagttca tattcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa caccacgttg
     1981 ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg aatcgatgac
     2041 tcttcccttc gaacgtaata acgcgcctgg ctcaaaggcc agcgttgggt cagtcagact
     2101 tccacttagc tgcgcgggta agcgggcata taaaatgagg tccgcctgtt tcgcttcacc
     2161 ggtcagctgc agaggcagct ggctgttatc catgcttaat tttcctgggc caaaatttag
     2221 caccgcgttg cctttacccg cttgcccctg ggtcagcaca ctcagtcgtc cgctgatcag
     2281 agcgttctca agccctgcct gccagttgtc gactttgaca cccagtcggc cactcaaagg
     2341 aaaacctgca tacggccaac tccagcgacc atcgcttacg gtcaattgtt gacgagtaat
     2401 ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacaa tcaattgccc
     2461 gctattttcc tgccagtcca gctctgcatc caccagtgac ggttcctgcg gcaagtttaa
     2521 cgtcgcagta gcatgcccac ttacaggaag tccatccggc acgagcggca tagtaaattc
     2581 ccccaccagt ttaaccggcg gctgattttc aaacgcgatg acatccagtt cgctgactgt
     2641 aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat aacgcagttg
     2701 ctgaatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc agggagaaaa
     2761 aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact gcgcgagggt
     2821 ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag cagaatcaag
     2881 ttctaccatg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg cgttggtgat
     2941 atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta aacctttacg
     3001 cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc agatgcccgc
     3061 cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa gaataatcag
     3121 taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat atcctgattt
     3181 agcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc agttgaaggt
     3241 tgcgcctaca ctaagcattg ttgttgatga atttttcaat atcgccatag ctttcaatta
     3301 tatttgaaat tttgtaaaat atttttagta gcttaaatgt gattcaacat cactggagaa
     3361 agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta cctgcaacag
     3421 gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac ggaaaaaacc
     3481 gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga cggcagccgc
     3541 ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg ctgtgccggt
     3601 ttcaataacg tcgaccttga cgcggcaaaa gaactggggc tgaaagtagt ccgtgttcca
     3661 gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac gctgaaccgc
     3721 cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga aggtctgacc
     3781 ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat cggtgtggcg
     3841 atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt
     3901 gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt ctctgaatca
     3961 gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt gaacgaagcc
     4021 gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt
     4081 gattctcagg cagcaattga agcgctgaaa aatcagaaaa ttgggtcgtt gggtatggac
     4141 gtctatgaga acgaacgcga tctattcttt gaagataaat ccaacgacgt gatccaggat
     4201 gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca ccaggcattc
     4261 ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt aagcaatctg
     4321 gaaaaaggcg aaacctgccc gaacgaactg gtttaatctt gccgctcccc tgcattccag
     4381 gggagcttat tcagataatc cccaaagacc tttcatcttc tattcttaaa atagccctga
     4441 gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt gcgctaagcc
     4501 tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa cagctacagc
     4561 atcatcgctt tgtgctggaa agcgtaaacg gtaagcccgt gaccagcgat aaaaatccgc
     4621 cagaaatcag ctttggtgaa aaaatgatga tttccggcag catgtgtaac cgctttagcg
     4681 gtgaaggcaa actgtctaat ggtgaactga cagccaaagg gctggcaatg acccgtatga
     4741 tgtgcgctaa cccgcagctt aatgaactcg ataacaccat tagcgaaatg ctgaaagaag
     4801 gtgcacaagt ggatctgacc gcgaaccagt taacgctggc gaccgcgaaa cagacattaa
     4861 cttataagct ggcggattta atgaattaat agctgccaca gctcccggcg gcaagtgact
     4921 gttcgctaca gcgtttgccg ttgggtaatg cacacatccc aatcgccgta ccatccagtt
     4981 gacgggcaac agaaagcgaa ccgccgatca ttgcacaatt tgcttctcca ctactggaca
     5041 tcgacgcttt taaacctggc gctacgtgcg ccgcagtcgc ctgctgaaca ggttcactac
     5101 tacacgccga caacaataaa gcggcacacc ctacccaaaa cgctgctcgc atctcttctt
     5161 cctctgatct tcaagccaaa cgacaccgcc ataaataata ggcagcacag agggcggcgt
     5221 cgagagctgt cctgcgcgtt gccccgcatt tttacttttt tatggctatt tttttgccct
     5281 ctgtttgatc aaaacattca ttacgctgat gtgggggaca caaaagcgaa aatgcagaag
     5341 aaagccattt gctaaaattg aaggattact actgggcgcg cagcaatttc gtgcgcccct
     5401 catttgcgca atgtaagggt gtcatatgat tactattgat ggtaatggcg cggttgcttc
     5461 ggtggcgttt cgcaccagtg aagttatcgc catctaccct attaccccca gttccacgat
     5521 ggcagaacag gctgatgcct gggccggaaa cggcttgaag aacgtttggg gagacacacc
     5581 atgcgtggtt gaaatgcagt cggaagcggg tgctatcgct accgtgcatg gcgctttgca
     5641 gacgggtgcc ctttcaacat cgtttacgtc atcgcagggt ttgctgctga tgatcccgac
     5701 actgtacaaa ctggcaggcg aactcacacc gtttgtcctg catgtagcgg cacgtaccgt
     5761 tgccacacat gcactctcta tttttggcga tcattccgac gtcatggcgg tgcgccagac
     5821 gggttgcgcg atgttgtgtg cggcaaacgt ccaggaagcg caagactttg ctctcatttc
     5881 gcacatcgcg acgctgaaaa gccgcgtgcc atttattcat ttctttgatg gtttccgcac
     5941 gtcccacgaa atcaacaaaa ttgtcccgct ggccgatgac acgattcttg atctcatgcc
     6001 gcaggccgaa attgatgctc atcgcgcccg ggcgctcaac ccggaacatc cggtgatccg
     6061 tggtacgtcc gccaatcctg acacttattt ccagtctcgc gaagccacca acccctggta
     6121 caacgcagtc tatgaccatg ttgaacaggc gatgaatgat ttctctgccg cgacaggtcg
     6181 tcagtatcag ccgtttgaat attacgggca tccgcaagct gaacgggtga ttatcctgat
     6241 gggctcagcc attggcacct gtgaagaagt ggttgatgaa ttgctaaccc gtggcgaaaa
     6301 agtcggcgtg ctgaaagttc gcctgtaccg ccccttctca gccaaacact tgctacaaac
     6361 tctaccggga tccgtacgca gcgtggcggt actggacaga accaaagaac ccggtgccca
     6421 ggcagaaccg ctctatctgg atgtgatgac cgcactggca gaagccttta ataatggcaa
     6481 gcgcgaaact ctgccccgtg tcattggtgg gcgctatggt ctttcatcca aagaatttgg
     6541 cccggactgt gtactggcgg tatttgccga gctcaacgcg gctaaaccga aagcgcgctt
     6601 tacggttggt atttacgatg atgtaaccaa tctgtccctg ccgttgccgg aaaacaccca
     6661 gccaaactcg gcgaaacttg aagccttgtt ttatggcctt ggtagtgatg gcagcgtttc
     6721 cgcgaccaaa aacaatatca agattatcgg taattccacg ccgtggtacg cacagggcta
     6781 ttttgtttac gactccaaaa aagcgggcgg cctgacggtt tctcaccttc gcgtgagcga
     6841 acagccgatt cgttccgctt atctcatttc ccaggctgat tttgttggct gccaccagtt
     6901 gcagtttatc gataaatatc agatggctga gcgtttaaaa cctggcggca ttttcctgct
     6961 caacacgccg tacagcgcag atgaagtgtg gtcgcgcttg ccgcaagaag ttcaggccgt
     7021 gttaaaccag aaaaaagcgc gcttctatgt gattaacgcg gcgaaaatcg cccgcaaatg
     7081 tggcctggcg gcccgtatta ataccgtcat gcagatggct tttttccatc tgacgcaaat
     7141 tctgcctggc gatagcgccc tcgcagaatt gcagggtgcg attgccaaaa gttacagtag
     7201 caaaggccag gatctggtgg aacgcaactg gcaggctctg gcgctggcgc gtgaatccgt
     7261 agaagaagtt ccgttgcaac cggtaaatcc gcacagcgcc aatcgaccgc cagtggtttc
     7321 cgatgccgcc cctgatttcg tgaaaaccgt aaccgcagcg atgctcgcag ggcttggtga
     7381 tgccctcccc gtttcggcgc tgccgccaga cggcacctgg ccgatgggca ccactcgctg
     7441 ggaaaaacgc aatatcgccg aagagatccc catctggaaa gaggaactct gtacccaatg
     7501 taaccactgc gtcgcagctt gcccacactc agctattcgc gcaaaagtgg taccacccga
     7561 agcgatggaa aacgcccctg ccagcctgca ttcgctggat gtgaaatcgc gtgatatgcg
     7621 cgggcagaaa tatgtcttgc aggtggctcc ggaagattgc accggttgta acctgtgcgt
     7681 cgaagtttgc ccggcgaaag accgtcagaa tccagagatt aaagccatca atatgatgtc
     7741 gcgcctggaa catgtcgaag aagagaaaat caattacgat ttcttcctca acctgccaga
     7801 aatcgaccgt agcaaactgg aacgtattga tattcgtaca tcgcagctga ttacaccgct
     7861 gtttgaatat tccggtgctt gctccggttg tggcgagacg ccgtatatta aattgctgac
     7921 tcagctctat ggcgaccgga tgttgatcgc taacgccacc ggctgttctt caatttatgg
     7981 cggtaacctg ccctctacac cgtataccac cgatgccaac ggtcgtgggc cggcatgggc
     8041 gaactctcta tttgaagata atgccgaatt tggccttggt ttccgcctga cggtcgatca
     8101 acaccgtgtc cgcgtgctgc gtctgctgga tcaatttgcc gataaaatcc cggcggaatt
     8161 actgacggcg ttgaaatcag acgctacgcc agaggttcgt cgagaacagg tcgcagcttt
     8221 acgccagcaa ctcaacgatg ttgccgaagc acatgaactg ctacgtgatg cagatgcact
     8281 ggtggaaaaa tcaatctggc tgattggtgg tgatggctgg gcttacgata tcggctttgg
     8341 cggtctggat catgtattga gtttgacgga aaacgtcaac attctggtgc tggatacgca
     8401 atgctattcc aacaccggtg gtcaggcgtc gaaagcgaca ccgctgggtg cagtaactaa
     8461 atttggcgag cacggcaaac gtaaagcgcg taaagatctt ggcgtcagta tgatgatgta
     8521 cggtcatgtt tatgtggcgc agatttctct cggcgcgcag ctgaaccaga cggtgaaagc
     8581 gattcaggaa gcggaagcgt atccgggacc atcgctgatc attgcttata gcccgtgtga
     8641 agagcatggt tacgatctgg cactcagcca tgaccagatg cgccaactca cagctaccgg
     8701 cttctggccg ctatatcgct tcgatcctcg tcgtgccgat gaaggcaaac tgccgctggc
     8761 gctggattca cgcccgccgt cagaagcact ggaagaaacg ttacttcacg agcaacgttt
     8821 ccgtcgtctg aattcgcagc agccagaagt ggcagaacag ttatggaaag atgctgcagc
     8881 tgatttgcaa aaacgctatg acttcctggc acaaatggcc ggaaaagcgg aaaaaagcaa
     8941 caccgattaa atgctctgga taaggattac ccaattctaa aaaaaagccc ggacgactgt
     9001 tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc aacagaaaat
     9061 aaaatctgtg acaataaagg catataaccc gcgcagaata acgtatagaa aattaaaagt
     9121 ataattttta ttttgtatgt attatcttcg atacattcac cagacttatt atcatttcag
     9181 aagaattatt tgattaaggt tttacttaag gcgtaacaaa tgatatttat cggctaactg
     9241 aacttctcct ttaggatgtt ttcactcccc ggaatgggga taaaaggcaa ataaaataac
     9301 taaaggattt attcaatgaa aagcaaagta ctggcacttt taattcctgc cctgctcgcc
     9361 gcaggtgctg cacatgcagc cgaagtttat aataaagacg gcaacaaatt agatctgtat
     9421 ggcaaagttg atggcctgca ttatttttct gataattcag cgaaagatgg cgatcagagc
     9481 tatgcgcgtc tgggttttaa aggcgaaacc caaattaacg atcaactcac tggctacggt
     9541 caatgggaat acaatattca ggcaaacaac actgaatctt caaaaaacca gtcatggacc
     9601 cgtctggcat ttgccgggct gaaatttgca gattacggtt ctttcgatta cggacgtaat
     9661 tatggcgtaa tgtacgacat cgaaggctgg accgatatgc tgcctgaatt tggcggtgac
     9721 tcttatacca atgcagacaa ctttatgact ggtcgtgcca acggcgtcgc gacttatcgt
     9781 aatactgatt tcttcggtct ggtaaatggt ctgaacttcg cggtgcagta tcaaggtaac
     9841 aacgaaggtg ccagtaatgg tcaggaaggc accaacaacg gacgtgatgt tcgccatgaa
     9901 aatggtgacg gctggggtct ttccacaaca tatgatttag gcatgggctt tagcgctggt
     9961 gcggcataca cctcatctga ccgcaccaat gaccaggtta accatactgc ggcgggtggt
    10021 gataaagcag acgcgtggac tgctgggcta aaatacgatg ctaacaatat ttacctggca
    10081 accatgtatt cagaaacgcg taatatgacc ccgtttggcg acagcgatta tgctgttgca
    10141 aacaaaaccc agaactttga agtcactgca cagtaccagt ttgattttgg tctgcgtccg
    10201 gcagtctctt tcctgatgtc taaaggccgt gacctgcacg ctgccgatgg tgcagacaac
    10261 ccggcaggtg ttgatgataa agatcttgtt aaatacgccg atgttggcgc gacttactat
    10321 ttcaataaaa acatgtccac ttatgttgac tataaaatca acctgttgga tgaagatgac
    10381 agtttctacg ctgccaacgg catctctacc gatgatattg tcgctttagg tctggtttat
    10441 cagttctaaa tcctcctgcc cgctgttatg gcgggctttt tctgcttatt cttcctcctt
    10501 tgatctaaat taaaaatgtg aactccgtca ttacacaaaa agtgtcatct ggcgttacac
    10561 tttatgcgga tactaaaaca ggaggtttta tgaacagaac gattcttgtc cctatcgata
    10621 tttccgattc agaattaact caacgcgtga ttagccacgt tgaggcagag gcaaagattg
    10681 acgatgcaga ggttcatttc ctgacggtaa taccttcact gccttactat gcctctctgg
    10741 gtttagcgta ttccgcagaa ttaccggcaa tggatgacct gaaagcggaa gccaaatcgc
    10801 aactggaaga gatcattaaa aaatttaaac tgccaaccga cagagtgcat gtccatgttg
    10861 aggaaggctc gcccaaagac cgcattctgg aattggcgaa gaagatcccc gctcatatga
    10921 tcatcattgc ttcccatcga ccggatatca ccacttatct gctcggttcc aacgccgcag
    10981 ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg ctgacactaa cgcccgcaca
    11041 ttgctgcggg ctttttgatt catttcgcaa atgtgctgac attttcccct ctaatccgta
    11101 ccatacacgc cacagtttta tatcagattt cttatgctgg gcgttccggc atgacgcata
    11161 ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat caagaaatta
    11221 gtaagaaaga acaatacaac ctgaacaagt aagggcaaaa atcacaacta tctgacccat
    11281 aagtatttta tttatttttc aatatgttaa acattatcag cgacaacaat aagctacgat
    11341 aacccactct ttttttgccc cttttttgcc ccttctacag cgttttgccc catttttgcc
    11401 atcaacaatt ttcctcataa agtcatttca aagcctaata gctgctctac ttttttcctc
    11461 aatatcatgt cgccagcagg aaacaaccta atccctgttt taggcctaaa agctgttaat
    11521 gataatcatt ttcattttgt atgggttatg ctataaaaca tagccgttgc tatgtttgtt
    11581 ctaattttgc tcactatagg tactaaatta tgcactcaat aaaaaaagta accatgctct
    11641 tggggtgcct cgccctcacc tgctcgatcg catttcaggc aagtgcaact gaaaaattca
    11701 aggtcattac aacatttacc atcatcgcgg atatggccaa aaacgtggct ggagatgctg
    11761 cagaagtctc atccataacc aagcctggtg cagaaattca tgagtatcag cctacccctg
    11821 gcgatattaa acgtgcgcag ggggcacaac tgattctcgc caatggtatg aatctggaat
    11881 tgtggttcca acgcttttac cagcatctca atggggttcc agaagtaatt gtctcttcgg
    11941 gtgtgacgcc agtagggatc accgaaggac cctatgaggg caaacctaat ccccatgcat
    12001 ggatgtcgcc agataatgct ctgatttacg tcgataatat tcgtgatgcg ttgataaaat
    12061 acgatccggc aaatgcacaa acctaccaac gtaatgccga tacttataaa gccaagatta
    12121 cccaaaccct tgcccccctg cgtaagcaga ttacggaact ccctgagaat cagcgatgga
    12181 tggtcaccag tgaaggggct ttttcttatc tcgcacgcga tttggggcta aaagagcttt
    12241 atctgtggcc gattaatgcc gatcaacaag gaacaccgca gcaggtacgt aaggttgttg
    12301 atatagttaa gaaaaatcat atcccggcag tctttagcga gagtacgatt tccgataaac
    12361 cagcgcgtca ggttgcgcgt gaaaccggcg cacactacgg cggtgtgctc tatgtcgatt
    12421 ccctgagcac agaaaacggc ccggtaccta cgtatatcga ccttcttaag gttactacca
    12481 gtacgctggt acagggaatt aaagccggga aaagggagaa ataatgatgc aatctgcagg
    12541 cattgtcgtt aatgatgtta ctgtcacctg gcgtaacggg cacacagcac tgcgcgatgc
    12601 atccttcaca gtaccaagtg gatctattgc cgctctggtt ggggtaaacg gttccggaaa
    12661 atcgacgttg tttaaagcga ttatgggatt tgtgcgtctg accagcggaa agatatctgt
    12721 tctgggtatt cccacgcgac aggcgctaca gaaaaacctg gttgcctacg tccctcagtc
    12781 agaagaggtt gactggtcat ttcctgtact ggtggaagat gtggtgatga tgggccgcta
    12841 tgggcatatg ggtatgttgc gtatagccaa aaagagggat cgtcagattg tcaccgatgc
    12901 gctggaacgt gtcgatatgg tggattttcg ccatcgtcaa atcggcgagc tttccggtgg
    12961 acaaaagaaa cgcgtctttc tggcgagagc gattgcacag cagggtgatg tgatcctgct
    13021 ggatgaacct tttaccggcg ttgatgtgaa aaccgaagcg aaaatcatca gcctgttgag
    13081 ggagttacgc gcggaaggca aaacgatgct tgtctcaacg cacaatctcg gctcagtcac
    13141 gacattttgc gattatacgg tcatggtcaa aggcaccgtc ctggcaagcg ggccgacaga
    13201 caccactttt acagccgaga acctggagct ggcttttagc ggcgtactgc gccatgtcac
    13261 acttaacgac tcggaagaaa gcattattac tgacgatgaa cgcccttttg tggcacatcg
    13321 accgtcagcg gtgcagaggg aagcaagatg aacgtgctac tggaaccctt cagctatgaa
    13381 tatatgctca atgcgatgtg ggtctcggca atggtcggcg gtctctgcgc tttcctgtca
    13441 tgctatctga tgctaaaagg ctggtcgctg attggcgatg cgctgtcgca ctctattgtc
    13501 ccgggtgttg cgggggcata tatgctgggg cttccgtttt ctctgggagc cttcttttcc
    13561 ggcggactgg cagcgggcag catgctgttt cttaaccaac gcacccgctt aaaagaagat
    13621 gccatcatcg gcctgatctt ctcgtccttt tttggcctgg ggctgtttat ggtgtcattg
    13681 aacccgacat ccgtgaacat tcagaccatc gtcctgggca atattctggc aatcgatccg
    13741 gcggatatcc tgcaactcac tatcattggc attctctcga tcatcgtgct ttttttcaaa
    13801 tggaaagatc ttatggtgac ctttttcgac gagaatcacg cccgtgctat tggattacat
    13861 ccgggcagat taaaaatcct cttcttcacc ctgctgtcgg tttcgaccgt tgcggcgctg
    13921 caaacggttg gtgcctttct ggtgatctgt ctggtggtga ctcccggtgc aacagcatgg
    13981 ttgttaaccg atcgttttcc ccgcctgctc ataattgctg taactattgg cagtgtaacc
    14041 agtttcctcg gcgcgtgggt cagttacttt ctggacggcg cgacaggtgg aatcatcgtg
    14101 gtggcgcaga cactgctgtt tcttttagca tttgtatttg cgcccaccca tggacttttg
    14161 gcaaatcgtc ggcgtgcgca taaagcgctg gaggatcgct catgatggca ttattactgg
    14221 agccactgca gttcaccttt atgagtcacg cactgctgat ttcactggta gtttccatac
    14281 cctgtgcgct gctgtcggtc tttctggtcc tgaaaggttg ggcactgatg ggtgacgcga
    14341 tgagtcatgc ggtttttcct ggcatcgtgc ttacctggat tctggggtta cctctggcta
    14401 caggggcatt cgtcgccgga gtattctgcg cggtggcgac gggatacctg aaagacaaca
    14461 gccgaatcaa gcaagataca gttatgggga ttgttttttc tggcatgttt gccgcaggtc
    14521 tgatcttgta tattgcaatc aaaccagacg tacatcttga ccatattctt ttcggcgaca
    14581 tgctcgggat aaccatcggc gatataatcc agacggtgat tattgccggg ctggttacac
    14641 ttgttattag cgcaaaatgg cgggattttt tgctgttcag ctttgattat caacaggcgc
    14701 aggtaagcgg tttgcatacg agatggctgc attatgggct gctgtgcatg gtttccctga
    14761 ccattgtggc gacgctgaaa gcagtaggta tcattctctc tatttctctg ctcattgcgc
    14821 ctggcgccat tgcagtactc ctgacacaac gctttcatat tgcgctccta ctggcgactg
    14881 gcatatcggt aatagtgtca atgactggtg tctggctgtc cttttttatc gacagcgcac
    14941 cagctccgac gattgtcgtc ttattcgcgg tcgtgttcat catgacgttt accgttacca
    15001 gcatcaacgc acgcacaaag ggaaacgccc atacacagga tctgttatca cccaattagc
    15061 acaacatccg aggacagcat cgtccgccag cctacagaag tccggaaagc catggaaggt
    15121 cgttgggagg aggtacgaat tctatcatgc aaaaaatacg taaaatcgat aacgcctaga
    15181 aatcattcaa tactcgcact atcgaaagtt taccagccaa ccgcggcacg ttcctgcata
    15241 cgacgtgccg cagcttctgt tattccacga taaaattcct aaccgaattc aaatccttca
    15301 gactattcag ctcttccttc agctcccgct gacggcgata aatctcgtca ttgcgctcaa
    15361 cctgtgcctg agccattgct gccgccagtt cttccagttc cggcatcgac tgtttcacct
    15421 gctgattatc ggcatcaccc cacgtcatca cgtcccgtac aatgtcggat ttcgcagcca
    15481 ttaccaccgg ataaaggcgg cccagtgagt tggggccagc attccagata cgaccgttcc
    15541 attcaaacgt gaacggcttc gcctcctgtt ctgtgcgcca tg
//
LOCUS       DAJVXP010000098        15532 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVXP010000098 DAJVXP010000000
VERSION     DAJVXP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.098"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..2015
                     /locus_tag="OQS10_002902"
     CDS             <1..2015
                     /locus_tag="OQS10_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2293877.1"
                     /translation="SYYVSQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKT
                     NNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSK
                     QNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEAD
                     GSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSDFVQAGYQYGFNNLLTLY
                     GGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQT
                     STRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSAN
                     MSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHE
                     EKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYG
                     VNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLA
                     NRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEA
                     ELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVV
                     GQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            2031..3065
                     /locus_tag="OQS10_002903"
     CDS             2031..3065
                     /locus_tag="OQS10_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCS2293878.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3147..3485)
                     /gene="rcnB"
                     /locus_tag="OQS10_002904"
     CDS             complement(3147..3485)
                     /gene="rcnB"
                     /locus_tag="OQS10_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS2293879.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3704..4528)
                     /gene="rcnA"
                     /locus_tag="OQS10_002905"
     CDS             complement(3704..4528)
                     /gene="rcnA"
                     /locus_tag="OQS10_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS2293880.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4649..4921
                     /gene="rcnR"
                     /locus_tag="OQS10_002906"
                     /pseudo
     CDS             4649..4921
                     /gene="rcnR"
                     /locus_tag="OQS10_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5013..5109
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5144..5932
                     /gene="thiM"
                     /locus_tag="OQS10_002907"
     CDS             5144..5932
                     /gene="thiM"
                     /locus_tag="OQS10_002907"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS2293881.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5929..6729
                     /gene="thiD"
                     /locus_tag="OQS10_002908"
     CDS             5929..6729
                     /gene="thiD"
                     /locus_tag="OQS10_002908"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS2293882.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6794..7612
                     /locus_tag="OQS10_002909"
     CDS             6794..7612
                     /locus_tag="OQS10_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS2293883.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7664..8410
                     /locus_tag="OQS10_002910"
     CDS             7664..8410
                     /locus_tag="OQS10_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS2293884.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8384..9349)
                     /locus_tag="OQS10_002911"
     CDS             complement(8384..9349)
                     /locus_tag="OQS10_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS2293885.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9346..10350)
                     /locus_tag="OQS10_002912"
     CDS             complement(9346..10350)
                     /locus_tag="OQS10_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS2293886.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10347..11624)
                     /locus_tag="OQS10_002913"
     CDS             complement(10347..11624)
                     /locus_tag="OQS10_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2293887.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11881..12933
                     /gene="fbaB"
                     /locus_tag="OQS10_002914"
     CDS             11881..12933
                     /gene="fbaB"
                     /locus_tag="OQS10_002914"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS2293888.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13252..14106
                     /gene="gatY"
                     /locus_tag="OQS10_002915"
     CDS             13252..14106
                     /gene="gatY"
                     /locus_tag="OQS10_002915"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS2293889.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14135..15392
                     /gene="gatZ"
                     /locus_tag="OQS10_002916"
                     /pseudo
     CDS             14135..15392
                     /gene="gatZ"
                     /locus_tag="OQS10_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15402..>15532
                     /locus_tag="OQS10_002917"
     CDS             15402..>15532
                     /locus_tag="OQS10_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS2293890.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4076 a         3720 c         3972 g         3764 t
ORIGIN      
        1 cctcttatta tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta
       61 catatgtacg ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca
      121 gtttcagtaa aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac
      181 gtggatttgc ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata
      241 tttttgattc tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta
      301 actcgaaaca aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa
      361 ctattgaaca gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga
      421 ttacagattt gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg
      481 acggttcggt aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg
      541 gcgtgtcgaa atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata
      601 gtgattttgt ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg
      661 gctcgatggt tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca
      721 ttggtgccat ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt
      781 ttgacgggca aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt
      841 ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt
      901 gggcaaacaa taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt
      961 attaccagaa tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc
     1021 cagaaggttg ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg
     1081 gcagtagtaa ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc
     1141 tcgcggcaag ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta
     1201 tatcgattcc ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt
     1261 ctaactcaac gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa
     1321 cagtagggag tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa
     1381 atgaaacgac agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca
     1441 gttatagtca gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg
     1501 cctggtcggg tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg
     1561 cgccaggaat taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg
     1621 gagtggtggt atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt
     1681 cgcaaagcga tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg
     1741 gcgcggttgt actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt
     1801 taagagcgga tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata
     1861 atattggcgt tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat
     1921 cggttaatgt agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag
     1981 agattgatga aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc
     2041 gcataatgct atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata
     2101 gtgagttatc tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac
     2161 aaatagcgac atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca
     2221 cccctatgag ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt
     2281 tacattttga atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca
     2341 ccagtggtta ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg
     2401 cggggggcgg cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat
     2461 cgacacgcgg gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg
     2521 atgcatgtgt aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta
     2581 atctgacatt gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta
     2641 ctttagacag tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata
     2701 gtaaacaagg ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat
     2761 cacggaaaat gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc
     2821 tgaaaggtgc agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca
     2881 catttttaaa tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa
     2941 agtcaaaact tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg
     3001 atacaaacaa agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg
     3061 actaataaca agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt
     3121 tccccctcat gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct
     3181 ttaatgattt taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag
     3241 tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca
     3301 tcggtgcggt acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg
     3361 aaactactta actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt
     3421 gcggcccagg cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg
     3481 gtcataaact ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca
     3541 atataactga caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga
     3601 aaggatgccc taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga
     3661 atcatcctgg gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca
     3721 tgaagccatg tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg
     3781 gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga
     3841 ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga
     3901 ccagtgttgc gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg
     3961 ctgccgggca ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga
     4021 cctctctacc atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct
     4081 gatactcgcc atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt
     4141 gatgatgatg ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc
     4201 aggtacgcca gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga
     4261 gccacggttc tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa
     4321 ttaaccagac cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt
     4381 taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt
     4441 ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt
     4501 gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct
     4561 gattctactc cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt
     4621 ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact
     4681 gaaagcgcgt gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga
     4741 gccgcatgaa tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg
     4801 tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga
     4861 gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata
     4921 atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca
     4981 ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt
     5041 gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg
     5101 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct
     5161 gggttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
     5221 ctgcatgacc aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc
     5281 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc
     5341 cttgttgatt aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt
     5401 tgagcaagca aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct
     5461 cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg
     5521 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac
     5581 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
     5641 aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat
     5701 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
     5761 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
     5821 ctggatgaaa caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt
     5881 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
     5941 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt
     6001 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
     6061 aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc
     6121 gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc
     6181 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc
     6241 gacaccgtta tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg
     6301 ctacgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc
     6361 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
     6421 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
     6481 agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
     6541 accaaaaaca cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg
     6601 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta
     6661 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
     6721 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
     6781 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg
     6841 cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta
     6901 ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc
     6961 tttcgctatt cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca
     7021 ggagcggatc gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt
     7081 tgcctttatt aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
     7141 gcaactaagc cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt
     7201 agcagcaccg gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt
     7261 ccctgcggta ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag
     7321 ggtaagtcag tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc
     7381 aggagccgtt ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt
     7441 ggctcactac tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
     7501 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
     7561 cacggtggag gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag
     7621 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
     7681 ccagctgatt gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat
     7741 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
     7801 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
     7861 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
     7921 gatcaacaac atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt
     7981 gttgcgcggg aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga
     8041 agaagtcgcg cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat
     8101 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
     8161 tcatgatgtt gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta
     8221 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
     8281 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
     8341 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
     8401 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
     8461 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
     8521 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
     8581 atgccggaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
     8641 gaacgatcaa cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg
     8701 cggataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag
     8761 gtcgacaggc catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa
     8821 aatcgataaa cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc
     8881 agggcgaggc cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc
     8941 gcgccagcca ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt
     9001 cgccatccgg ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca
     9061 ggctgattaa gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac
     9121 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
     9181 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
     9241 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
     9301 gacgcgtggt taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg
     9361 ttgttgacga tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
     9421 atccagttcc gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat
     9481 tgccgtcgcc atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc
     9541 gcagcgattc gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc
     9601 aatagtgctg gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
     9661 gtcggcattg cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa
     9721 cgtagtgatg cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc
     9781 tgaccagctt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
     9841 aaccgccaga tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga
     9901 atcaacatca cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
     9961 ggtcacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
    10021 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc
    10081 gagaatatta cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc
    10141 cgccagacac agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc
    10201 cgcgttattc tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac
    10261 gcggctgcgt ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata
    10321 aagagcaccg agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg
    10381 cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac
    10441 agcacggcga taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa
    10501 gtcagtccgt ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga
    10561 cgatagccga gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg
    10621 gtacgcatat gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa
    10681 tcgtaactta cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca
    10741 tccgtactac cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat
    10801 aatacctttt tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt
    10861 tcagagaact ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga
    10921 taaccgttgg caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag
    10981 aaaaagacga ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact
    11041 ttaatatcca ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca
    11101 ccgagcagag cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg
    11161 gcatacccca gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata
    11221 gtgcccatca cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg
    11281 atgctgttag tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga
    11341 aagaacccgg caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac
    11401 atcaataccg ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga
    11461 attggcgaca ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca
    11521 ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac
    11581 cattcaacaa acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat
    11641 gacaggtaat gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt
    11701 gcggtgaaaa cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct
    11761 gaaaaaacat ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc
    11821 aaaaggcggg ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta
    11881 atgacagata ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt
    11941 atgacaattc cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg
    12001 attgacaata atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg
    12061 cgtctggctg gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct
    12121 gccggagctt catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg
    12181 gcgatcgaag cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg
    12241 cggcgctatg cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt
    12301 tacccgaata cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc
    12361 gcggtggcgg ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa
    12421 gaaatttctg cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc
    12481 tatttgcgaa actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg
    12541 accggtcagg caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg
    12601 gcggaaaata acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat
    12661 agcaaattga ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat
    12721 atgggccggg ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt
    12781 gatgcagtgc gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga
    12841 cgtaaagcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac
    12901 gtttatctcg atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc
    12961 gtaggacgga taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag
    13021 cgtcttatca tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc
    13081 cggcataaaa acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct
    13141 ttcactcctc gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa
    13201 caaatcattt cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg
    13261 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
    13321 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
    13381 ccggtcatca tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg
    13441 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
    13501 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
    13561 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    13621 tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat
    13681 gatgtgcaag tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt
    13741 gccgaggcaa ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat
    13801 gccagcgcac cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta
    13861 ccgctggtgc tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa
    13921 ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta
    13981 aaaaattacc tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct
    14041 aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg
    14101 gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa
    14161 agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc
    14221 ggcgctggca tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca
    14281 ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt
    14341 tacgattgcc gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct
    14401 ggggccaaac tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct
    14461 ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg
    14521 cgcgggggat cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg
    14581 ctgcggaaag tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg
    14641 aagttccggt tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg
    14701 aagatgccgc caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag
    14761 aggcgttaac acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca
    14821 atattatcca ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc
    14881 gaatggttta tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag
    14941 tccgcgatca ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg
    15001 cgatatttgc actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt
    15061 gcctggcggt aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc
    15121 gtacgggttt taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt
    15181 attactggcc gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag
    15241 gcgtggacat cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc
    15301 agtccgggga attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt
    15361 tgcgcgccta tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc
    15421 gtagcggaat ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc
    15481 tcgccaaagg tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAJVXP010000099        15405 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVXP010000099 DAJVXP010000000
VERSION     DAJVXP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.099"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            8..451
                     /locus_tag="OQS10_002918"
     CDS             8..451
                     /locus_tag="OQS10_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2293891.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            667..756
                     /locus_tag="OQS10_002919"
                     /pseudo
     CDS             667..756
                     /locus_tag="OQS10_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(757..1815)
                     /gene="modC"
                     /locus_tag="OQS10_002920"
     CDS             complement(757..1815)
                     /gene="modC"
                     /locus_tag="OQS10_002920"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS2293892.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1818..2507)
                     /gene="modB"
                     /locus_tag="OQS10_002921"
     CDS             complement(1818..2507)
                     /gene="modB"
                     /locus_tag="OQS10_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS2293893.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2507..3280)
                     /gene="modA"
                     /locus_tag="OQS10_002922"
     CDS             complement(2507..3280)
                     /gene="modA"
                     /locus_tag="OQS10_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2293894.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3446..3595)
                     /gene="acrZ"
                     /locus_tag="OQS10_002923"
     CDS             complement(3446..3595)
                     /gene="acrZ"
                     /locus_tag="OQS10_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS2293895.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3724..4512
                     /gene="modE"
                     /locus_tag="OQS10_002924"
     CDS             3724..4512
                     /gene="modE"
                     /locus_tag="OQS10_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS2293896.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4580..6052
                     /gene="modF"
                     /locus_tag="OQS10_002925"
     CDS             4580..6052
                     /gene="modF"
                     /locus_tag="OQS10_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS2293897.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6313..7329
                     /gene="galE"
                     /locus_tag="OQS10_002926"
     CDS             6313..7329
                     /gene="galE"
                     /locus_tag="OQS10_002926"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS2293898.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7339..8385
                     /gene="galT"
                     /locus_tag="OQS10_002927"
     CDS             7339..8385
                     /gene="galT"
                     /locus_tag="OQS10_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS2293899.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8389..9537
                     /gene="galK"
                     /locus_tag="OQS10_002928"
     CDS             8389..9537
                     /gene="galK"
                     /locus_tag="OQS10_002928"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS2293900.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9531..10571
                     /gene="galM"
                     /locus_tag="OQS10_002929"
     CDS             9531..10571
                     /gene="galM"
                     /locus_tag="OQS10_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS2293901.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10774..11526
                     /gene="gpmA"
                     /locus_tag="OQS10_002930"
     CDS             10774..11526
                     /gene="gpmA"
                     /locus_tag="OQS10_002930"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS2293902.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11685..12737)
                     /gene="aroG"
                     /locus_tag="OQS10_002931"
     CDS             complement(11685..12737)
                     /gene="aroG"
                     /locus_tag="OQS10_002931"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS2293903.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13053..13433
                     /locus_tag="OQS10_002932"
     CDS             13053..13433
                     /locus_tag="OQS10_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS2293904.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13547..14488
                     /gene="zitB"
                     /locus_tag="OQS10_002933"
     CDS             13547..14488
                     /gene="zitB"
                     /locus_tag="OQS10_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS2293905.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14485..15204)
                     /gene="pnuC"
                     /locus_tag="OQS10_002934"
     CDS             complement(14485..15204)
                     /gene="pnuC"
                     /locus_tag="OQS10_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS2293906.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15242..>15405)
                     /gene="nadA"
                     /locus_tag="OQS10_002935"
     CDS             complement(15242..>15405)
                     /gene="nadA"
                     /locus_tag="OQS10_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS2293907.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3849 g         3596 t
ORIGIN      
        1 cctttgcatg gatcttgcac tggtaacagg acagcgcgaa ggagatctgg cggctatgag
       61 atgggaggat ataagagatg gcaggctata cgtggaacaa caaaaaacag gggcaaaaat
      121 tcgcatttct ctccccacca cgatttccag attgaattta acacttgccg atgtactgga
      181 taacttgaaa aaaataaacg gtaaaaacga aaagttactc ggagggaaga cagccaggac
      241 aatcgcagca cagttcagga ttgcccgaga cacatccgga ttgaaatggg aaggtgaccc
      301 accgccattt catgaaatcc gaagtttatc cggtcgcctg cactcagcag aaaaaggcgg
      361 tgatttcact caggcgctcc ttggacatcg ttcatccagc atgacggata aataccgtga
      421 cggacgaggg agggaatgga aagacattta aaattaattt cgaatgattt cgaaacacca
      481 cttgtaatta attgattata aatgattaat tcctaatggg catttgcttc atgaatgctc
      541 ctttgcattt agcaactgac tgtaacagct aaaattagtc gcttttggcg gtgaatgctg
      601 aattaaaacg cagaattttg tccgatagtg gtatcggtgt accattcgcg cagacgaaaa
      661 ttcttaaatg acgcggtaaa agcgcgcgcc aacattgtga ttggtgataa caccaccgac
      721 agcattgccc agttcattta tagccacctg atttaatcag gcggttatcg acacactttt
      781 aatttgcgcg tacagccaca ggccaggttt gatcgccagt tcatccctgg cccacgggct
      841 gatacgcgcc cacagcgttt taccgccgac ttccagctcc acttccacct ggccgttgtc
      901 gtcataacta ttaacaactt ttgcccgcaa tacgttacga atgctggttt gctgcggcgg
      961 ttgtaaaacc agagaaacat cggaagcctg aatgcggata cgtagcgcag cttgcagcgg
     1021 ttcgtccagc ttattgaccc acaaatgctg atcgcccagc gccagcgcgg tcatcgcgta
     1081 atgcggatga tgctcaagta ccgtcacttt cagaatgcta ctttgttgct ctttcggcag
     1141 ccacggattc atcacgctac tgccccacac ttcctccagc gcgccaaagg ctttcacctg
     1201 accgttttcc agtaccatca ctctgtctgc cagatggagg atctcatcca gcgaatggct
     1261 gacatacaac atcggaatgt tgatttcccg tgtcagccgt tgcagataag gcaacagttc
     1321 gcgtttacgc ggaatatcca gtgacgccag cggttcatcc agcaacagca attccggtgc
     1381 tgtcagcaaa gcccgaccaa tcgccacgcg ctgtttttcg cctccggaca ggctgcctgg
     1441 taaacggtca agcaacggtt caatgcctaa aagcgccacc agcttatcga actgatcgac
     1501 catacttttc gacatgccgt agcgcagatt gccacgcact ttgtaatgcg ggaacagccg
     1561 cgcatcctga aaaacatagc caacgcgacg cttttccggc gtcaggcaga tacctttttc
     1621 ggcatcattt agtacccgcc cattgaggac aatccgccct ttttgcgggc gcgtcagtcc
     1681 actgatggcg ttaatcagcg aagtttttcc ggcaccggag acgccaaaga tagcagtgat
     1741 gccattggcg ggcagtgttt cattaatggt caggcaatgg ttgcccaacg tctgggaaaa
     1801 attcagctcc agcatgatta gcgccccgcc cgttcacggc tgattctggc cagccattct
     1861 gaaatcaaca gggagatcat cgccagcgca atagaaataa tgcacagtct cgccgcgcca
     1921 ctttccccgc cgggggtctg gatcagggta tacatggcag agggaatggt tcgcgtttcg
     1981 ccaggaatgt tcgacacaaa ggtgatggtt gcaccaaact caccgagaga acgagcaaaa
     2041 gccagtaccg taccaacaat aattcccggt aaggtcagcg gtaacgtgat agtaaagaaa
     2101 acgcgccagc gcccggcacc tagtgttctt gcggcctgtt cgagtttgac atcaacccct
     2161 tccagcgcca gacgaattgc ccgcaccatc agcggaaacg acatgacggc ggcagcgaga
     2221 accgcgccgc gccagctaaa ggcgaaggta ataccaaacc agtcatacag acgttcaccg
     2281 ataaatccgc gccgtcccat cgaaactaat aataagtaac cgacgaccac gggcggtaac
     2341 accagcggta gatgcagtac gctgtcgagc agagctttgc ccggaaacgt gcaacgcacc
     2401 agtaaccagg caaaaaagat cccaaacggc aggctaaaca gcacagccag ggaagaaact
     2461 ttcaggctta ataaaactgc ctgccattct ggatcggtca gtatcattac ttggttgtaa
     2521 atccgtaacg tttaaagatt tccgctgcct gcggtccctt cagataatca taaaatgctt
     2581 tcactgtcgc attgttatgc ccttccacaa cagcaaccgg atattccact tttttatgtg
     2641 aatcttccgg gaaggtggca accactttta cccctttgct gacaactgcg tcagaaccgt
     2701 aaacaatgcc caacggcgct tcattgcgtt cgaccaacgc cagcgcgcca cgaacatctt
     2761 ccgccggagc cagtttcggg gagagcgtat cccatgcgcc cagtttttgc agtgcttctt
     2821 ttgcataaat gccagcaggg acatgttccg gatcgccaac cgcgaggcga ccgccattca
     2881 gcagcgaagt ccagttagtt gtgctgtcga tgatgaaatc tttctgctcg ctggctttcg
     2941 gtgctacaac gaccaggcta ttgccgagca gtgtctggcg cgaagccgta tcgatcgctt
     3001 ttttatcaac cgcataatcc atccatttct gatcggcaga gataaacaga tccgcaggcg
     3061 ctcccgcttc aatctgacgg gccagagtag atgacgaagc gaaagaagaa accacatcca
     3121 cgcctttttc ttttttatac tgcgtagcaa tgtcctgcat tgcgttagtc agtgatgcgg
     3181 cggcgaacac cgtgattttc ccttcatctg ccagtgtatt gccagcaaca gcgaaagaga
     3241 gtgccgcccc ggcaaacaag ttcaaccatt tacgagccat tggtaacccc ttaatgtaac
     3301 gttatatagg tgacaatgta acggctagct cagaattttc ccagcagtta ttgctaacct
     3361 ttgtgaggta gataagaaaa aatatcggct ggaaaagcag gaagttgaga aaaagaaatg
     3421 cccgactaag cgggcattca gggaatcaat gattttgtcc gggctggtct tttttaccaa
     3481 caccagaaaa gatgttgaat acttcaccaa gaccgtaaat cagacccagg atgatggcca
     3541 tcacgacagg taccatgatt acggcgaata ccagactttt taataactct aacatggtca
     3601 actccagata tagtcatgag actattctaa ccgctaagca cagaaaagca ctcccctttt
     3661 gtgcggtcag ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg ccaggacatt
     3721 gttatgcagg ccgaaatcct tctcaccctt aagctccaac aaaaattatt cgccgacccg
     3781 cgccggattt cgctactaaa acacattgcg ctttccggtt ccattagcca gggagcgaaa
     3841 gatgccggta tcagctataa aagcgcctgg gatgccatta acgagatgaa tcagttaagc
     3901 gagcatattc tggttgagcg cgcaacaggc ggtaaaggtg gcggcggcgc agtactgacc
     3961 cgctatggtc agcgactgat tcagctctat gacttactgg cgcaaatcca gcaaaaagcc
     4021 tttgatgtgt taagtgacga tgacgccctg ccgctgaaca gcctgctggc cgcgatctca
     4081 cgtttttcac tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac cgcccgcgat
     4141 catgatgaca ttcaacagca tgttgatgtc ttactggctg acggaaaaac acgcctgaaa
     4201 gtcgcaatta ccgcacaaag cggcgcacgt ctggggctgg atgaaggcaa agaagtgttg
     4261 atattgctaa aagcgccgtg ggtaggcatt actcaggacg aagcggtcgc acaaaacgcc
     4321 gacaaccaat taccgggtat tattagtcat attgagcgcg gcgcagagca gtgcgaagta
     4381 ttaatggcgc tacccgacgg gcaaacactg tgcgccacag tgccggtaaa tgaagcgact
     4441 tctcttcagc aagggcagaa tgtcacggcc tactttaatg ccgacagcgt gattatcgcc
     4501 acgctgtgct aagcgtgttg acaatttgtt atgaaacacg tatccctgtc agtaatcgct
     4561 gcacaaaatg ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttag
     4621 cgacacaaaa acgctgcaat tgcctcagct aacgttaaac gcgggtgata gttgggcgtt
     4681 tgtcggttcg aatggaagcg ggaaatcggc cctggcccgc gcgctggcgg gggaacttcc
     4741 gcttttgaaa ggtgaacggc aaagccagtt ttcccacatc actcgtctct ccttcgagca
     4801 attgcaaaaa ctcgtcagcg acgaatggca gcggaataac accgatatgc tcggccctgg
     4861 cgaagatgac accggacgca ctacggctga aatcattcag gatgaagtaa aggatgcacc
     4921 gcgttgcatg caactggcgc agcagttcgg tattaccgcc ctcctcgacc gacgctttaa
     4981 atacctttcc actggcgaga cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc
     5041 tgacttgttg attcttgatg agtcgttcga tggcctggat gttgcctcac gtcagcagct
     5101 ggctgagcga ctcgcctcgt tacatcagtc cggtattact ctggtactgg tgctcaatcg
     5161 cttcgatgag atcccggagt ttgtccagtt tgctggcgtg ctggcggatt gcacgttagc
     5221 ggaaactggc gctaaagagg aactgctcca acaagcactc gtcgcgcaac tggcgcatag
     5281 tgaacagctt gaaggtgtgc aactgccgga gccggatgaa ccttcagcac gtcacgcctt
     5341 acccgccaac gaaccgcgca ttgtgctgaa caatggcgtg gtttcttata acgatcgccc
     5401 cattcttaat aaccttagct ggcaggtgaa tccaggtgaa cactggcaaa ttgtcgggcc
     5461 aaatggtgca ggaaaatcga cgttattaag cctggttact ggcgatcatc cgcaaggtta
     5521 cagcaacgat ttgacgcttt tcggacgacg tcgcggcagc ggcgaaacca tctgggatat
     5581 caaaaagcat atcggttacg tcagcagtag tttgcatctg gattaccggg tcagcactac
     5641 cgtgcgtaat gtgattcttt ctggctattt tgattcgatt ggcatttatc aggccgtttc
     5701 ggatcgccag caaaaactgg tgcagcagtg gctggatatt ctcggcattg ataaacgtac
     5761 ggctgacgct ccgttccata gtctttcctg gggacagcag cgtctggcgc tgatcgtccg
     5821 cgcactggtg aaacatccga cgttgcttat tctcgatgaa ccactacagg ggcttgatcc
     5881 gctcaatcgc cagcttatcc gccgttttgt tgatgtgctg attagcgaag gtgaaacgca
     5941 attgttattt gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc atcgccttga
     6001 gttcgtgccg gacggtggac tctatcgcta tgtgctgaca aaaatatatt gagtcggtag
     6061 tgctgacctt gccggaggcg gcctaagcgc cctctccggc caacggttcg acgcatgcag
     6121 gcatgaaacc gcttcttttt tcagataaaa agcgcaatca ttcataaacc ctctgtttta
     6181 taatcactta atcgcgcata aaaaacggct aaattcttgt gtaaacgatt ccactaattt
     6241 attccatgtc acacttttcg catctttgtt atgctatggt tatttcatac cataagccta
     6301 atggagcgaa ttatgagagt tctggttacc ggtggtagcg gttacattgg aagtcatacc
     6361 tgcgtgcaat tactgcaaaa cggtcatgat gtcatcattc ttgataacct ctgtaacagt
     6421 aagcgcagcg tactgcctgt tatcgagcgc ttaggcggca aacatccaac gtttgttgaa
     6481 ggcgatattc gtaacgaagc gttgatgacc gagatactgc acgatcacgc tatcgacacc
     6541 gtgatccact tcgccgggct gaaagccgtc ggcgaatcgg tacaaaaacc gctggaatat
     6601 tacgacaaca atgtcaacgg cactctgcgc ctgattagcg ccatgcgcgc cgctaacgtc
     6661 aaaaacttta tttttagctc ctccgccacc gtttatggcg atcagcccaa aattccatac
     6721 gttgaaagct tcccgaccgg cacaccgcaa agcccttatg gcaaaagcaa attgatggtg
     6781 gaacagatcc tcaccgacct gcaaaaagcc cagccggact ggagcattgc cctgctgcgc
     6841 tacttcaacc cggttggcgc gcatccgtcg ggcgatatgg gcgaagatcc gcaaggcatt
     6901 ccgaataacc tgatgccata catcgcccag gttgctgtag gccgtcgcga ctcgctggcg
     6961 atttttggta acgattatcc gaccgaagat ggtactggcg tacgcgatta catccacgta
     7021 atggatctgg cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa gccaggcgta
     7081 cacatctaca acctcggtgc tggcgtaggc agcagcgtgc tggacgtggt taatgccttc
     7141 agcaaagcct gcggcaaacc ggttaactat cattttgcac cgcgtcgcga aggcgacctt
     7201 ccggcctact gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg cgtaacgcgc
     7261 acactcgatg aaatggcgca ggacacctgg cactggcagt cacgccatcc acagggatat
     7321 cccgattaag gaacgaccat gacgcaattt aatcccgttg atcatccaca tcgccgctac
     7381 aacccgctca ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg cccctggcag
     7441 ggggcgcagg aaacgccagc caaacaggtg ttacctgcgc acgatccaga ttgcttcctc
     7501 tgcgcaggta atgtgcgggt gacaggcgat aaaaaccccg attacaccgg gacttacgtt
     7561 ttcactaatg actttgcggc cttgatgtct gacacgccag atgcgccaga aagcaacgat
     7621 cctttgatgc gttgccagag cgcgcgcggt accagccggg tgatctgctt ttcaccggat
     7681 cacagtaaaa cgttgccaga actgagcgtt gcggcattga cggaaatcgt caaaacctgg
     7741 caggagcaaa ccgcagagct gggaaaaacg tacccgtggg tgcaggtctt tgaaaacaaa
     7801 ggtgcggcga tgggctgctc taacccgcat ccgcacggac agatttgggc aaatagcttc
     7861 ctgcctaacg aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc cgaacagaaa
     7921 tcaccaatgc tggtggatta tgttcagcgc gagctggcag acggtagccg taccgttgtc
     7981 gaaaccgaac actggttagc cgttgtgcct tactgggctg cctggccgtt cgaaacgcta
     8041 ctgctgccca aagcccacgt tttgcggatc accgatttga ccgacgccca gcgcagcgat
     8101 ttggcgctgg cgttgaaaaa gctgaccagt cgttatgaca acctcttcca gtgctccttc
     8161 ccctactcta tgggctggca cggcgcacca tttaatggcg aagagaatca acactggcag
     8221 ctgcacgcgc acttttatcc gcctctgctg cgctccgcca ccgtacgtaa atttatggtt
     8281 ggttatgaaa tgctggcaga gacccagcga gacctgaccg cagaacaggc agcagagcgt
     8341 ttgcgcgcag tcagcgatat ccattttcgc gaatccggag tgtaagaaat gagtctgaaa
     8401 gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca caccattcag
     8461 gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg tttcgttctg
     8521 ccctgcgcga ttgattatca aaccgtgatc agctgtgcac cacgcgatga ccgtaaagtt
     8581 cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcaa tgcgcccatt
     8641 gtcgcgcatg aaaactatca atgggcgaac tacgttcgtg gcgtggtgaa acatctgcaa
     8701 ctgcgtaaca acagcttcgg cggtgtggac atggtgatca gcggcaatgt gccgcagggt
     8761 gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt gcagcagctt
     8821 tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc agaaaaccag
     8881 tttgttggct gtaactgcgg gatcatggat cagctaattt ccgcactcgg caagaaagat
     8941 catgccttgc tgattgactg ccgctcactg gggactaaag cagtttccat gccgaaaggt
     9001 gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc tggttggcag cgaatacaac
     9061 acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc tctgcgcgat
     9121 gtcaccattg aagagttcaa cgctgttgca catgagctgg acccaatcgt ggcgaaacgc
     9181 gtgcgtcata tcctgactga aaacgcccgc accgttgaag ctgccagcgc gctggagcaa
     9241 ggcgatctga aacgtatggg cgagttgatg gcggagtctc atgcctctat gcgcgatgat
     9301 ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc agtgattggc
     9361 gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt cgcgctgatc
     9421 ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca
     9481 ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg
     9541 aaactcccgc actggcaccc gatggtcagc cgtaccgact gttaactttg cgtaacaacg
     9601 cagggatggt agtcacgctg atggactggg gtgcgacttt actttcagct cgtattccgc
     9661 tttccgatgg cagcgtccgc gaggcgctgc tcggctgtgc cagcccggaa tgctatcagg
     9721 atcaggccgc gtttctgggg gcctctattg gtcgttatgc caaccgcatc gccaatagcc
     9781 gttatacctt tgacggtgaa acggtgacgc tttcgccaag tcagggcgtt aaccagctgc
     9841 acggcggccc ggaagggttc gacaaacgtc gctggcagat tgtgaaccag aacgatcgtc
     9901 aggtgctgtt tgccctgagt tcagatgatg gcgatcaggg cttcccgggt aatctcggcg
     9961 cgacggtgca atatcgtctg accgacgata accgtatctc cattacttat cgcgccacag
    10021 ttgataaatc ttgcccggtg aatatgacta atcacgtcta tttcaatctt gacggcgagc
    10081 agtctgacgt gcgcaatcac aagttgcaga ttctggcggg cgaatatctg ccggttgatg
    10141 aaggcggcat tccgcacgac ggcctgaaat ctgtcgccgg aacctctttt gatttccgca
    10201 gcgccaaaat catcgccagt gagtttcttg ccgacgacga tcagcgcaaa gtgaaaggtt
    10261 acgatcacgc attcttgtta caggccaaag gcgatggcaa gaaagtggcg gcgcatgtct
    10321 ggtcagcaga tgaaaaattg cagctgaagg tctacaccac cgctcccgct cttcaattct
    10381 actccggcaa cttcctcggc ggcacaccgt cgcggggaac cgaaccttac gccgactggc
    10441 aaggactggc gttggaaagc gagtttctgc cggacagccc gaaccaccct gaatggccgc
    10501 aaccggactg cttcctgcgt cctggcgaag agtattccag cctgacggaa tatcagttta
    10561 ttgctgagta atgttaagcc ctcgtcatga gggctttatc ttcatattgt tcaaatcacc
    10621 agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg cgccactatt
    10681 ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtca cggcaatata atgagaatta
    10741 ttatcataaa aagataattt gaggagtaag tatatggctg taactaagct ggttctggtt
    10801 cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta cgacgtggat
    10861 ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa agaggaaggt
    10921 tacagctttg actttgctta cacttctgtg ctgaaacgcg ctatccatac cctgtggaat
    10981 gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa actgaacgaa
    11041 cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ccgccgaaaa gtatggcgac
    11101 gagcaggtga aacaatggcg tcgtggtttt gccgtgactc cgccggaact gactaaagat
    11161 gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa agagctgccg
    11221 ctgactgaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa tgaaactatt
    11281 ctaccgcgta tgaagagcgg tgagcgcgtg atcatcgctg cacacggtaa ctctttacgt
    11341 gcgctggtga aatatcttga taacatgagt gaagaagaga ttcttgagct gaatatcccg
    11401 accggcgtgc cgctggtgta tgagttcgac gagaatttca aaccgctgaa acgctattat
    11461 ctgggtaatg ctgacgagat cgcagcgaaa gcagcggcgg ttgctaacca gggtaaagcg
    11521 aagtaaacgt cattcgttta aaaatgagaa agccgactgc aagtgagtcg gcttttttgt
    11581 tgctaacaat ggagcacatt gcctgatgcg acgctgcgcg tcttatcagg cctgtggtga
    11641 ttcatcggat acgccactct gacggcgcat ccgacaatta aaccttaccc gcgacgcgct
    11701 tttactgcat tcgccagttg acgtaacaga gcatcggtat cttcccagcc aatgcaggca
    11761 tcggtgatgc tcttaccgta ggccagcggc tccccgctat cgaggctctg attgccttcc
    11821 accagatggc tttccaccat cacgccaata atggcctttt cgccaccggc aatctgctgg
    11881 caaacgtcag cacaaacatc catctgcttt ttgaattgtt tggacgagtt agcatggctg
    11941 aaatcgatca tcacctgtgc tggcaggcct gctttgttca gcccttcttt cacttcagca
    12001 acgtgcttcg cgctgtagtt aggctcttta ccgccgcgca gaatgatatg gcaatcaccg
    12061 ttaccgctgg tattcacaat cgccgaatgc ccccatttag ttacggacag gaagcagtgc
    12121 ggcgcaccgg cggcattaat ggcatcgata gccactttaa tcgtaccgtc tgtgccattt
    12181 ttaaaaccta ccggacaaga aagaccagac gccagttcgc ggtgcacctg cgattccgtg
    12241 gtacgtgcac caattgcgcc ccagctcatc aggtcagcga gatactgagg agtgatcata
    12301 tcgagaaact cacccgccgc tggcagaccg ctgtcgttaa tatcaagcag caatttacgg
    12361 gctatacgca gaccgtcgtt gatctggaag ctgttatcca tatgcggatc gttaatcaac
    12421 cctttccagc ccaccgtggt acgcggcttt tcaaaataga cgcgcattac gatttccagc
    12481 tcatctttca gctcttcacg cagcgccagc aagcgagtgg catactcttt agccgcgaca
    12541 ggatcatgaa ttgagcacgg gccaatcacc accaacaggc gatcatcatt acctttcagg
    12601 atcttatgga tcgcttttcg ggcatgggca accgtattcg cggcattttc agttacgggg
    12661 aatttttcca gcaatgcgac aggaggaagt aactctttga tttctttgat gcgtaaatcg
    12721 tcgttctgat aattcatgtc tgttccagtg ttgccatact tatcttagtg aatgcaatac
    12781 ttccaatcta tatcttccgt cagaatgtgt aaacggggtt ttacactatg aacggattaa
    12841 tcctggaatt agcaagaaaa acgccagatt gtcgcgaaaa acgagatctc tcctacaatt
    12901 tctaactgta actcctttgt ttgttaatta tttcaagatt ctctgctgcg tttcataaac
    12961 tggctgaaat cttaaaccaa tgccttatat tcacctgcaa atgcactgtt ggaagaggtt
    13021 atccgacata acgaccataa caggagcatc ctatgaaaat gacaaaactg gccacattat
    13081 ttctgactgc cactctaagc cttgccagcg gtgccgcact ggctgccgat agcggagcgc
    13141 aaactaataa cggccaggca aacgccgcag ctgatgcggg ccaggtagcc cctgacgccc
    13201 gtgaaaatgt cgcgccaaat aacgtcgaca ataacggggt aaataccggt tctggcggca
    13261 caatgctgca ttcggatggt tcttcaatga acaatgacgg aatgaccaaa gatgaagagc
    13321 acaagaacac catgtgcaaa gatggtcgat gtccggatat caataaaaag gtacaaatcg
    13381 gtgatggcat caataatgat gtcgatacca aaaccgacgg taccacgcag taaccctgca
    13441 aaatagccaa taatgggaga gcggagtctc tcccttttaa tttttcactt ctaaaagcag
    13501 tatgatgtca taaagttaag tgagaataat taagggatga ctcgtcatgg cgcactcaca
    13561 ctcacacacg tcctcacacc tgccagaaga taataatgct cgtcgcttgt tgtatgcttt
    13621 cggcgtgact gccgggttta tgctggtaga agtcgttggt ggttttcttt ctggttctct
    13681 ggcattgctg gccgatgcgg gtcatatgtt gaccgatact gccgccctgc ttttcgccct
    13741 actcgccgtg caattttcac gtcgtcctcc caccattcgc cacactttcg gttggctaag
    13801 actgaccacg ctcgcggcgt ttgtgaatgc catcgccctc gtggtaatta ccattttgat
    13861 tgtctgggag gcgatagaac gcttccgcac gccgcgtccg gtcgagggcg gcatgatgat
    13921 ggcaattgcc gtggctgggt tgctggcaaa tatactttct ttctggttac ttcatcacgg
    13981 cagcgaagag aaaaacctca acgtacgagc agcggcattg catgtgctcg gggacctgtt
    14041 gggttcggtt ggggctatta ttgccgcgtt gattattatc tggaccggct ggacacctgc
    14101 tgaccccatt ctctcgatag tggtgtcgct tctggttctg cgtagcgcgt ggcgactgtt
    14161 gaaagatagc gtgaatgaat tacttgaagg tgcaccggta tcgctggata tcgctgaact
    14221 gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat gtgcaccatg tacatgtatg
    14281 gatggtaggc gagaagccgg tgataacgct gcatgtgcag gtgatcccgc cacacgatca
    14341 cgatgccttg ttggatcaga tccaacacta cctgatggat cactatcaga ttgagcacgc
    14401 caccattcag atggaatatc aaccttgtca tgggccagac tgccacctga atgaaggggt
    14461 gtctggccat tcacatcatc accattaatg ggacagcgcg cgtgagcctc tttcacgtgc
    14521 gctgttgatc cacatccgac tgccgttgag cgcaataaag gtcaggatga tgtactccag
    14581 agacatggcg taaacgccct gaagtgcaaa aataacgacg ctaatcacgt taataatcac
    14641 ccacaacagc cagttttcca catacttacg cgtcatcaga atcattgcca cgatagataa
    14701 caccatcatg catgaatccc agaacgggaa agcgtccggt tgcagttcag gcatcacaac
    14761 ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg gtcaaaaatg caaacaccgg
    14821 attgataaat accgtcatca gaccaatcga aacaacgcaa accgccaacc agctgagcgc
    14881 cttcggcaat ggtaaccagc gaattttcaa ctccgcctcg ttctgactgg tttgtcgcga
    14941 ccacgcatac caaccgtaaa tattcgcggc aaagaaaaac acctgtaata gcaggctggc
    15001 atacagctga atctgaaaga aaataatgcc aaacaaggtg acgttgatca ggccaaagaa
    15061 gtagttgctg atcttctcca gactggcaag gccaatacac agcaacccgg cgatcgtgcc
    15121 taccgcttcg atccatgaga gatcataacc gcctgcccct attggtatat gtaccaggat
    15181 attctgcaca ctaaaaaaat ccatctttcc cccaaagcgt aacgttacgc cttattattc
    15241 gttatccacg tagtgtagcc gcaaaatcca gcatacgatt gagcggcacc agcgccctct
    15301 ctcgcagcct ttcatcaaga acctcgtgat tgcttccttc ctgttctaat gcctctgcga
    15361 tggactgaag gccattcatg gccatccacg gacaatgcgc gcagc
//
LOCUS       DAJVXP010000100        15235 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVXP010000100 DAJVXP010000000
VERSION     DAJVXP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.100"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..749
                     /locus_tag="OQS10_002936"
                     /pseudo
     CDS             1..749
                     /locus_tag="OQS10_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(790..1722)
                     /locus_tag="OQS10_002937"
     CDS             complement(790..1722)
                     /locus_tag="OQS10_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS2293908.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OQS10_002938"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OQS10_002938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS2293909.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OQS10_002939"
     CDS             complement(5254..5886)
                     /locus_tag="OQS10_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS2293910.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OQS10_002940"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OQS10_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS2293911.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OQS10_002941"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OQS10_002941"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS2293912.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OQS10_002942"
     CDS             complement(8729..8839)
                     /locus_tag="OQS10_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS2293913.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQS10_002943"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OQS10_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS2293914.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQS10_002944"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OQS10_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS2293915.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQS10_002945"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OQS10_002945"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS2293916.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OQS10_002946"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OQS10_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS2293917.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3801 c         3914 g         3555 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaataaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttattt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgct
//
LOCUS       DAJVXP010000101        15084 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVXP010000101 DAJVXP010000000
VERSION     DAJVXP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.101"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..781
                     /locus_tag="OQS10_002947"
     CDS             <1..781
                     /locus_tag="OQS10_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293918.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OQS10_002948"
     CDS             complement(984..1337)
                     /locus_tag="OQS10_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS2293919.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OQS10_002949"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OQS10_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS2293920.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OQS10_002950"
     CDS             complement(3165..3458)
                     /locus_tag="OQS10_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS2293921.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OQS10_002951"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OQS10_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS2293922.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OQS10_002952"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OQS10_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS2293923.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OQS10_002953"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OQS10_002953"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS2293924.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OQS10_002954"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OQS10_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS2293925.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OQS10_002955"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OQS10_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS2293926.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OQS10_002956"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OQS10_002956"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS2293927.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OQS10_002957"
     CDS             10838..11413
                     /locus_tag="OQS10_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2293928.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OQS10_002958"
     tRNA            11520..11595
                     /locus_tag="OQS10_002958"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OQS10_002959"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OQS10_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="OQS10_002960"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="OQS10_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS2293929.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAJVXP010000102        14965 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVXP010000102 DAJVXP010000000
VERSION     DAJVXP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.102"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OQS10_002961"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OQS10_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS2293930.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OQS10_002962"
     CDS             complement(1608..1931)
                     /locus_tag="OQS10_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS2293931.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQS10_002963"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OQS10_002963"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS2293932.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQS10_002964"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OQS10_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS2293933.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQS10_002965"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OQS10_002965"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS2293934.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQS10_002966"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OQS10_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS2293935.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OQS10_002967"
     CDS             7531..8568
                     /locus_tag="OQS10_002967"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS2293936.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQS10_002968"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OQS10_002968"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS2293937.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OQS10_002969"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OQS10_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS2293938.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQS10_002970"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OQS10_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS2293939.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OQS10_002971"
     CDS             10739..11620
                     /locus_tag="OQS10_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2293940.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQS10_002972"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OQS10_002972"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS2293941.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OQS10_002973"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OQS10_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS2293942.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OQS10_002974"
     CDS             complement(14017..>14965)
                     /locus_tag="OQS10_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCS2293943.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3784 c         3922 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJVXP010000103        14615 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVXP010000103 DAJVXP010000000
VERSION     DAJVXP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.103"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..696)
                     /locus_tag="OQS10_002975"
     CDS             complement(<1..696)
                     /locus_tag="OQS10_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2293944.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="OQS10_002976"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="OQS10_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS2293945.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="OQS10_002977"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="OQS10_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS2293946.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="OQS10_002978"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="OQS10_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS2293947.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="OQS10_002979"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="OQS10_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS2293948.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="OQS10_002980"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="OQS10_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS2293949.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="OQS10_002981"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="OQS10_002981"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS2293950.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="OQS10_002982"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="OQS10_002982"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS2293951.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="OQS10_002983"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="OQS10_002983"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS2293952.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="OQS10_002984"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="OQS10_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS2293953.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="OQS10_002985"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="OQS10_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS2293954.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="OQS10_002986"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="OQS10_002986"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS2293955.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="OQS10_002987"
     CDS             10119..10499
                     /locus_tag="OQS10_002987"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS2293956.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="OQS10_002988"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="OQS10_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS2293957.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OQS10_002989"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OQS10_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS2293958.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="OQS10_002990"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="OQS10_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="OQS10_002991"
     CDS             complement(12845..14032)
                     /locus_tag="OQS10_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS2293959.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..14328)
                     /locus_tag="OQS10_002992"
     CDS             complement(14032..14328)
                     /locus_tag="OQS10_002992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293960.1"
                     /translation="MKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFFVDAGNKK
                     LVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPDGVAPPF"
     gene            14473..>14615
                     /locus_tag="OQS10_002993"
     CDS             14473..>14615
                     /locus_tag="OQS10_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2293961.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKT"
BASE COUNT         3690 a         3670 c         3766 g         3489 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttacg ttgtcagcgc cagtgatata agacggtaat
    14401 tcgccatttg gattgtccgc tccacccaac atgttgtttc cttaaggttc tcacaccaga
    14461 aaggacatca acatgctgag cagagaggac ttttacatga taaagcaaat gcgccagcag
    14521 ggcgcgtaca tcgtcgatat tgcgactcag attggttgct ctgaacggac ggtcagacgc
    14581 tacctcaaat accctgaacc gccagccaga aagac
//
LOCUS       DAJVXP010000104        14492 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVXP010000104 DAJVXP010000000
VERSION     DAJVXP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.104"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OQS10_002994"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OQS10_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OQS10_002995"
     CDS             1909..3261
                     /locus_tag="OQS10_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCS2293962.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OQS10_002996"
     CDS             3285..3767
                     /locus_tag="OQS10_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCS2293963.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..4750
                     /locus_tag="OQS10_002997"
                     /pseudo
     CDS             3811..4750
                     /locus_tag="OQS10_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4760..5239
                     /locus_tag="OQS10_002998"
     CDS             4760..5239
                     /locus_tag="OQS10_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293964.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5376..6161)
                     /locus_tag="OQS10_002999"
     CDS             complement(5376..6161)
                     /locus_tag="OQS10_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2293965.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6491..6567)
                     /locus_tag="OQS10_003000"
     tRNA            complement(6491..6567)
                     /locus_tag="OQS10_003000"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6531..6533),aa:Asp,seq:gtc)
     gene            complement(6700..7431)
                     /gene="dnaQ"
                     /locus_tag="OQS10_003001"
     CDS             complement(6700..7431)
                     /gene="dnaQ"
                     /locus_tag="OQS10_003001"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS2293966.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7496..7963
                     /gene="rnhA"
                     /locus_tag="OQS10_003002"
     CDS             7496..7963
                     /gene="rnhA"
                     /locus_tag="OQS10_003002"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS2293967.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7960..8682)
                     /locus_tag="OQS10_003003"
     CDS             complement(7960..8682)
                     /locus_tag="OQS10_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS2293968.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8716..9471
                     /gene="gloB"
                     /locus_tag="OQS10_003004"
     CDS             8716..9471
                     /gene="gloB"
                     /locus_tag="OQS10_003004"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS2293969.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9543..10901
                     /gene="mltD"
                     /locus_tag="OQS10_003005"
     CDS             9543..10901
                     /gene="mltD"
                     /locus_tag="OQS10_003005"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS2293970.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10950..11546)
                     /locus_tag="OQS10_003006"
                     /pseudo
     CDS             complement(10950..11546)
                     /locus_tag="OQS10_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11577..12377)
                     /locus_tag="OQS10_003007"
     CDS             complement(11577..12377)
                     /locus_tag="OQS10_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS2293971.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12618..13532
                     /locus_tag="OQS10_003008"
     CDS             12618..13532
                     /locus_tag="OQS10_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2293972.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13529..14332)
                     /gene="dkgB"
                     /locus_tag="OQS10_003009"
     CDS             complement(13529..14332)
                     /gene="dkgB"
                     /locus_tag="OQS10_003009"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS2293973.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         4005 a         3410 c         3443 g         3634 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaatactca gcccaataac atcatttgat tacattcatt ttatcactac
     4081 acatccttca ggtataaaag atacactcgc ctggttagtt aacgcgagta aattaatgac
     4141 tgagtttgat gacaatggga aaattatatt taacattaat gccttaaagt atacaaaagc
     4201 aagttacttt gaaatacttg gagagaaata tataaaaatc acaacatcca gcccctggct
     4261 tttagaaaag ctcggtaaat atattttctc ttcacgagcg cctcaagttc tggaactcgc
     4321 tattggctgg cgaggagctt tatctgaatc aataaaagga gttaagttct gtatctggtt
     4381 ttcagtagcc tggagaacca tagaatttat tatgagttca gagcgagatc tggttaattt
     4441 tcttggcgat ttttcaatgg atgtggccaa agcagttata gccggtggtg ttgcaaccgc
     4501 tattggaagc ctggcttctt ttgcctgtgt tagctttggc tttccagtaa ttcttgtcgg
     4561 aggagcaatt ttactgacag ggatagtgtg tacagttgtt ttaaatgaaa tcgatgctca
     4621 atgccattta tcagaaaaat taaaatatgc aattagagat ggactaaaac ggcaacagga
     4681 acttgataaa tggaaaaggg aaaacatgac tccatttatg tatgttctta acactccacc
     4741 cgtgatatga ggattaacca tgcgtttaca tgttaaatta aaagagtttc tttcgatgtt
     4801 ttttatggcc atattatttt tccctgcatt caatgcttct ttgtttttta ccggagttaa
     4861 acctctatat tcaataataa aatgctccac ggaaatattt tatgattgga gaatgttgat
     4921 tctgtgcttt ggtttcatgt cgttttcctt tttaaatatt catgtaattt tacttacgat
     4981 aataaaatta ttcttaatca aaaaaacaaa ggtagtcaat ttcgccacag acataacaat
     5041 acagctaact cttatatttt tactaatagc tatcgtaata gcacctttaa tagctccttt
     5101 tgtcacagga tatgtcaatg ctaattatca cccctgcggt aataatacgg gaattttccc
     5161 aggagcaatc tatattaaaa atggcatgaa atgtaataat gaatatattt ctcgcaagga
     5221 agattcagca gttaaataat taaacattct aaaacaaaca gtttttaact ccggattcca
     5281 tgtcctttct tgcactgtct ttgtcaagaa aaatttctta tgcaaagaat tttgtattat
     5341 ttccgccaga aattcctggc ggcagatata atttattatt gatagcggtt aaccaaatct
     5401 tttaccatcg ctttataggt cttttgctta agttgcatga tttgctgctc tgtcatatca
     5461 gtcgtactgg cttcattctt gtcgggcaat ggtggaagca ctttatattc ataattcacc
     5521 ggcgaccagg cagcccactc acctgtagct acatccacca gcgtgaatcg taccagatag
     5581 cgcagtgaca tcgtgtcagt caatttttga tttttataat ctgaccaaac tattgatttc
     5641 aatccagagt catattttcc tgtctgcaac gtatcctgga aaacaataat cgctttctga
     5701 tgcccttttg cagccacaaa acgcagtgcc tgcatccagt tcatattctc agcactggca
     5761 acatcctcgt tctcattttt atctttgttt ttgttacaag tcagaggctt ttgcctgtcc
     5821 gggataccag agaatgtgga aacagtataa tattttcgca tctcctcctg cataatggtt
     5881 tccggtgcgc ggttgccgga ttgaacaagt attacaggag aattaagggg aacgcggaac
     5941 tcatctccat caagcgcagc ttgaatatcc tcttcggata cggccagagt ggtttcatta
     6001 ccgaataaat ctttgtctgt cagttgctgg ggagtgtttt tattaatatt actttgtgtg
     6061 acttgagctt ttttcttctt ttcatcaata gaagattcag agacacatcc tgcaagcagc
     6121 gagaataata cacatataca acaaaccctt cttaaattca taatcggaca ctccctcgcc
     6181 tgtcataatc atggtaatgt ttagctaacg atttttaatt atatactcca cttgaatcag
     6241 aacaaacaaa aataacagta tatataaatt cattaaatca atagaaaaat gaaaatcata
     6301 acattcaaat atatttaaat tgaatgattt gatagtatga ttaaatatta atgatatggt
     6361 ctaccatatg tgattaacac atttacagca taacaaaacc atagaccgaa gaaatagcgg
     6421 gatttaaatg caaaaaaacc gcaaaattaa aaatcttgcg gttctctgaa ctcattttca
     6481 tgagtgaatt tggcggaacg gacgggactc gaacccgcgc ccccctgcgt gacaggcagg
     6541 tattctaacc gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt
     6601 acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa
     6661 atcgcccaag ccgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcagct
     6721 tccgcccttc ttctccacca gatcaagacg ggcttcatga gctgcaagct cttcatctgt
     6781 cgcaaaaaca acgcgtaact tacttgcctg acgtacaatg cgctgaattg ttgcttcacc
     6841 ttgttgctgc tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat
     6901 cgccagataa acttccgcaa ggatctgtgc atcgagtaat gccccgtgta gcgttcgttt
     6961 actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgct taccaggaaa
     7021 cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat
     7081 atcgcgctta agcaacgaaa actcgtagtc cataaagccg atatcgaacg ctgcgttatg
     7141 gatcaccaac tccgcgccgc ggatatagtc catgaactca tcggctactt cggcaaacgt
     7201 gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac
     7261 cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac
     7321 ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat
     7381 accggtggtt tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat
     7441 ttatgtcaga cttatcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct
     7501 taaacaggta gaaattttca ccgatggttc gtgtctgggc aatccaggac ctgggggtta
     7561 cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgcaggct acacccgcac
     7621 caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca
     7681 ttgcgaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat
     7741 ccataactgg aaaaaacgtg gctggaaaac cgcagacaaa aaaccagtaa aaaatgtcga
     7801 tctctggcaa cgtcttgatg ctgccctagg gcaacatcaa atcaaatggg aatgggttaa
     7861 aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat
     7921 gaatcccaca ctggaagata cgggctacca agtcgaagtt taagcttgtg gtttacgaca
     7981 ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg tttttactct gtttcatcgg
     8041 gtttagcgtt aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc
     8101 aggaatatgc gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg
     8161 gctggcgtgc agcacttcaa aattcaacaa agagagccag tccagttgcc gcatcagagt
     8221 aaacatccgg ctgttatagg gcgaggtttt tcgcaatacc ggcacaagtt tgcgtaatcc
     8281 cattaaacta atgggattga agccactaat caccagccag ccatcatcaa tcaatacccg
     8341 atcggtttca cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag
     8401 acaaacatca acggatttat cggcaaaagg aagatgaagt ggatccgcct gcacctggac
     8461 gggcattcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat
     8521 ttctgcgctt aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg
     8581 gttgagctgg cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagcg
     8641 atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac
     8701 actaagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg
     8761 gttttgaatg atgaagcagg tcgctgcctg attgtcgatc ccggagacgc agagccagta
     8821 ttaaacgcca ttgccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcat
     8881 cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat
     8941 ggtccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc
     9001 ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc
     9061 tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt
     9121 aggttgtttg aagggacagc atcacaaatg tatcaatcac ttaataagtt aagtgcgtta
     9181 cctgacgata cattggtatg ttgtgctcat gaatatacgt tatcaaatat gaagtttgct
     9241 ttgagtattc ttccgcacga tttgtccata aatgattatt atcgtaaagt taaggagtta
     9301 cgggcaaaaa atcaaataac actacccgta attctgaaaa atgagcggca aattaatgtt
     9361 tttttaagaa cggaagatat tgatttaatt aatgtaatta atgaagaaac attattgcaa
     9421 caacctgaag agcgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt
     9481 cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac
     9541 acatgaaggc aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg
     9601 gcaacgttca acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt
     9661 ttacaagtca ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt
     9721 gggctttcat tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac
     9781 agaaacagaa atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc
     9841 cgtatatgta ctggatagca gggcaagtta aaaaacgtaa catgcctatg gaactggtac
     9901 tactacccat agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag
     9961 gcatctggca gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt
    10021 atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc
    10081 tgaacaaaat gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag
    10141 gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt
    10201 cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata
    10261 ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc
    10321 tggcgcgtgt gcacctgagt agcccggttg aaatggcgaa ggttgcagat atggcgggga
    10381 tttccgtcag caagctgaag acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa
    10441 gtgggccgca gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctcg
    10501 cttcaggcga aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca
    10561 gccgtgttta caccgtacgc tctggcgaca cgctttcaag catcgcttca cgtctcggcg
    10621 taagcaccaa agatttgcag cagtggaaca aactgcgcgg atctaagctg aagccaggcc
    10681 aaagtttgac gattggtgca ggcagtagcg cacagcggtt ggcaaacaac agcgatagca
    10741 ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga
    10801 acatcaaaga tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc
    10861 tgacgttgtt tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaaa
    10921 ggcaccgatt cccccggtgc cttttttatt tatgccgctt tatgtgcttc taccatgatg
    10981 atatcactgg tgaaagagcc atcagtctgc aaggaaaaat acgttctcac ctctgtcgat
    11041 gcgctctgtt ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg cattctcacg
    11101 acccatgaag aaaattccag cggtaactta tctgtaatta aattatcaac tatcagattg
    11161 gcttcattga ttaacgtcaa ccacgcaccg ctggcgtagt ttcgtacgtg agaggtatcg
    11221 cgtaatgctt ctaccgcctg taaccagatg tcgcgcactg ggtgacccgg agacattaca
    11281 tccatcacaa tcagcctacc gccaggtttc aatatcctat tcacttctcg cagtgctgca
    11341 ccaacatcat gccagtgatg ggcagaataa cggctgataa caatatcaaa tgcgttatcg
    11401 gcaaatggca gacttgcggc atatccctgg cgggtggtga tatttttcag ttgccgggct
    11461 tcggcagctt gtgccacgac atccagcatg tgggcagata agtcatacgc caccaccgcg
    11521 ctcacgtttt gcgcagcgcc aaacgttggt ctgccctgcg gcagacctga cataccttat
    11581 ttatcaggct tgccgggact gaattcaacg agtagcggat tgtgatcgga agcgcgcgta
    11641 accagtacag aagcttcact gacgttcaga ccacggtaga aaacaaaatc gagcgggcga
    11701 ccaaacgccc gacggcgctg atcatcggta aaacgcacct ggcgcagcga catttcccgc
    11761 gcaaagcgat ataacgcgtt catccttcta cggctccagg cattgaaatc tcccgccata
    11821 atgaccgggc cgctgtggtg agctatctga tcaccaatag gaagtaactg cttactatag
    11881 acatccacgc ccagactgaa gttgacggcg tgtatattaa ccaccatcaa caggcgggtg
    11941 tcaggtaatg gatagaccgt caccagtgcc gacttcgcca gacgcaaaat gggttctcgt
    12001 tcacgtaacg ggcagcaata cactggatgt gccgccgaaa gggtcattac gccagaagga
    12061 tgttgtggca gcacgaaagc gggtacctga tcggcggcaa gatagttagc ggtcgcaaac
    12121 tgtaccaact ctggcgttgt ttgcgcttcc tgcaataaca ccagatgtgc gtctttgccg
    12181 taattcttta ataccgacaa ccattcagcg cgttgctgtt tgtatatgtt ccacaccagg
    12241 atccgaatac gctcttcggt acttaacggt tctccgggtg gtaatgcctg gccgatgctc
    12301 gcaaaagacc ccggcggtaa gatcctctcc gcaggttgtc cggcaacata gcgcatggca
    12361 taggtgtttt ttcgcacttt tgactttgaa acctctatta cgtcaaccct tccgcactcg
    12421 ggagggttct actgttatag ggattttagc tcactctttc aacgagcaat tgtatattgt
    12481 tatgtaagca agtgcttaca ccggtcagaa atttgctcat tattgagaac attaaatcgg
    12541 atgacatatc acgcatcctg atggtttaat gacaaataaa ttcaaaatat ctcatctatt
    12601 ttgcaacagg agcaaaaatg aaagccacgt cggaagaact cgccattttt gtttcggtcg
    12661 tcgaaagcgg cagctttagc cgggcagcgg aacaattagg gcaagcaaac tcagcggtaa
    12721 gccgggcggt gaaaaagctg gaaatgaaac ttggcgttag cctgcttaat cggaccacgc
    12781 ggcaacttag cctgacggaa gaaggcgagc gttattttcg tcgcgtacag tcaattttgc
    12841 aggagatggc agcggcagaa tcagaaatta tggagacgcg taatacaccg cgtggactgt
    12901 tacggatcga tgccgcaact ccagtggtgc tgcactttct gatgccgtta attaagcctt
    12961 tccgtgaacg ctatccggaa gtcactttat cgctagtctc ctccgaaacg attattaatt
    13021 tgatcgaaag aaaagtggat gtcgcgatac gcgctggtac gttaacggac tccagcttac
    13081 gtgccaggcc gttatttaac agttatcgaa aaattatcgc ctcccccgat tatatttccc
    13141 gctacgggaa gccagaaacg atcgacgatt taaagcaaca tgtttgcctg ggattcactg
    13201 aacccgcttc cctcaatacc tggccgatag cctgtagtga tggacaatta catgaggtga
    13261 agtacggttt gtcatccaat agtggggaaa cactgaaaca gctttgcctg agtggcaacg
    13321 ggattgcgtg tttgtccgac tacatgatcg acaaagaaat cgctcgcgga gaattggtgg
    13381 agttaatggc agataaagtg ttgccagtgg aaatgccatt cagtgccgtc tattacagcg
    13441 accgtgcggt aagtacgcgc atccgggctt ttatcgattt ccttagcgag catgtaaaaa
    13501 cagctcccgg aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc
    13561 taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct
    13621 gtaaattttg tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta
    13681 ctgagtaacc ttcccccata gcccacgcca gaatcacttg tgccggagtc gcattgtgtt
    13741 tagctgcaat acgagcaata acctcatctt tcagtgcctt accataagcc agcgtcatat
    13801 aggaagtgat atgaataccg tgctgtttag cccaggcaac cactttacgg ttttgcagat
    13861 aaggagagag ttcaatctgg ttggtagcga tgttttcagc gccaacagca gcaatcgctt
    13921 tttccatcaa cgggatcgtg aagttggaaa taccgatctc acgtgtcaga ccttgttttt
    13981 tggcctccag cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc
    14041 agtggattag cgtcagatca acataatcgg tacgcaattt ttgcaggctc tcttttagac
    14101 tcgggatcaa tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt
    14161 catgacgtgg cacgccactt tctgcaatcg cctgacctac tgcggcttcg ttatcataga
    14221 tttgtgcagt atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa
    14281 caacgtcgtc tttgagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc
    14341 tcttttaatg ctattttcgt taactgaggt gattatgggc ttcagactgt tgcggaaaaa
    14401 gagcataaaa tgcagaggat ttttgcgatt ctggcaataa tagatataca gacgaaaaaa
    14461 aacctcgagc gttaactcga ggcttcttaa ta
//
LOCUS       DAJVXP010000105        14404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVXP010000105 DAJVXP010000000
VERSION     DAJVXP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.105"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQS10_003010"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQS10_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS2293974.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQS10_003011"
     CDS             1828..3147
                     /locus_tag="OQS10_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS2293975.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OQS10_003012"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OQS10_003012"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS2293976.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OQS10_003013"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OQS10_003013"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS2293977.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OQS10_003014"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OQS10_003014"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS2293978.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OQS10_003015"
     CDS             8179..9729
                     /locus_tag="OQS10_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2293979.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OQS10_003016"
     CDS             9731..10753
                     /locus_tag="OQS10_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2293980.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OQS10_003017"
     CDS             10750..11646
                     /locus_tag="OQS10_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS2293981.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OQS10_003018"
     CDS             11643..12629
                     /locus_tag="OQS10_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2293982.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OQS10_003019"
     CDS             12658..13548
                     /locus_tag="OQS10_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2293983.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQS10_003020"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OQS10_003020"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS2293984.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVLGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3558 c         3629 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccagcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJVXP010000106        14306 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVXP010000106 DAJVXP010000000
VERSION     DAJVXP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.106"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OQS10_003021"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OQS10_003021"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS2293985.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OQS10_003022"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OQS10_003022"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS2293986.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQS10_003023"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OQS10_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCS2293987.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OQS10_003024"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OQS10_003024"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS2293988.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OQS10_003025"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OQS10_003025"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS2293989.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQS10_003026"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OQS10_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS2293990.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQS10_003027"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OQS10_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS2293991.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQS10_003028"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OQS10_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS2293992.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OQS10_003029"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OQS10_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS2293993.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OQS10_003030"
     CDS             complement(13987..>14306)
                     /locus_tag="OQS10_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS2293994.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJVXP010000107        14218 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVXP010000107 DAJVXP010000000
VERSION     DAJVXP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.107"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            17..153
                     /locus_tag="OQS10_003031"
     ncRNA           17..153
                     /ncRNA_class="other"
                     /locus_tag="OQS10_003031"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(244..930)
                     /locus_tag="OQS10_003032"
     CDS             complement(244..930)
                     /locus_tag="OQS10_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS2293995.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1511..2299)
                     /gene="phoH"
                     /locus_tag="OQS10_003033"
     CDS             complement(1511..2299)
                     /gene="phoH"
                     /locus_tag="OQS10_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS2293996.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2920..4191)
                     /gene="efeB"
                     /locus_tag="OQS10_003034"
     CDS             complement(2920..4191)
                     /gene="efeB"
                     /locus_tag="OQS10_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS2293997.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4197..5324)
                     /gene="efeO"
                     /locus_tag="OQS10_003035"
     CDS             complement(4197..5324)
                     /gene="efeO"
                     /locus_tag="OQS10_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS2293998.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5382..6212)
                     /locus_tag="OQS10_003036"
     CDS             complement(5382..6212)
                     /locus_tag="OQS10_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS2293999.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(6878..8386)
                     /gene="putP"
                     /locus_tag="OQS10_003037"
     CDS             complement(6878..8386)
                     /gene="putP"
                     /locus_tag="OQS10_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS2294000.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            8808..12770
                     /gene="putA"
                     /locus_tag="OQS10_003038"
     CDS             8808..12770
                     /gene="putA"
                     /locus_tag="OQS10_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS2294001.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12810..13448)
                     /gene="rutR"
                     /locus_tag="OQS10_003039"
     CDS             complement(12810..13448)
                     /gene="rutR"
                     /locus_tag="OQS10_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS2294002.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            13736..>14218
                     /locus_tag="OQS10_003040"
     CDS             13736..>14218
                     /locus_tag="OQS10_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS2294003.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         3513 a         3884 c         3524 g         3297 t
ORIGIN      
        1 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
       61 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
      121 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
      181 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
      241 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
      301 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
      361 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg cccattcatg
      421 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
      481 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
      541 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
      601 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
      661 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
      721 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
      781 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
      841 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
      901 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
      961 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     1021 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     1081 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     1141 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     1201 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     1261 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     1321 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     1381 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     1441 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     1501 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     1561 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     1621 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     1681 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     1741 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     1801 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     1861 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     1921 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     1981 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaagttt
     2041 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     2101 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     2161 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     2221 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     2281 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     2341 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     2401 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     2461 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     2521 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     2581 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     2641 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     2701 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     2761 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     2821 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     2881 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     2941 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     3001 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     3061 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     3121 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     3181 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     3241 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     3301 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     3361 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     3421 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     3481 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     3541 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     3601 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     3661 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     3721 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     3781 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     3841 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     3901 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     3961 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     4021 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     4081 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     4141 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     4201 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     4261 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     4321 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     4381 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     4441 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
     4501 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
     4561 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
     4621 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
     4681 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
     4741 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
     4801 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
     4861 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
     4921 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
     4981 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
     5041 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
     5101 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
     5161 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
     5221 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
     5281 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
     5341 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
     5401 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
     5461 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
     5521 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
     5581 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
     5641 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
     5701 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
     5761 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
     5821 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
     5881 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
     5941 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
     6001 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
     6061 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
     6121 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
     6181 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
     6241 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
     6301 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
     6361 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
     6421 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
     6481 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
     6541 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
     6601 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
     6661 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
     6721 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgact ggccgccccc
     6781 tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac attaagggag cgcaacccgc
     6841 tgctgaagcc aaacaaaacc gccgcaggct aagtccctta gttttcctgc aaccgtgacg
     6901 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
     6961 gcgctttacc cagcaaacta aacactacaa tcccgatact gccgaagatg aagcccggaa
     7021 taatttcgta cagtcccagc cagccgaact gcttccagac gataactgtc agcgcaccga
     7081 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca
     7141 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
     7201 ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc caccaccagc accatcacac
     7261 gtcctaccca caccagctct ttctggctgg cctgtttacg cagaaacgct ttgtataaat
     7321 cttcggtaat cgcactggag cacaccagca gctggcaact taaggttgac attaccgccg
     7381 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
     7441 cgataaacac gcgttcagcg ttctggttta ccgcacctgc taccgccgga tgctcgttaa
     7501 agtaagcgat accaaagaag ccaacagcca ccgcccctgc caggcaaagg atcatccagg
     7561 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atctgccgcc ataaaacgcg
     7621 ccaggatgtg cggctgcccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
     7681 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
     7741 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
     7801 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
     7861 aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc cgtttcgtaa ctcatgccaa
     7921 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
     7981 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
     8041 cggtgaaata atccggcaat gttaaggcgt tattattgta ttcggtatgc acacgcaacc
     8101 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
     8161 tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc cattaacagc cagccgctca
     8221 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
     8281 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
     8341 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
     8401 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
     8461 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
     8521 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
     8581 ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt aactgccgca gagaaaaagt
     8641 ctgagttatt ttttaatcac tgtcatatcg atttctttta ttaacatttc attcattttt
     8701 aagcttgcta cgcaagtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
     8761 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
     8821 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
     8881 tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
     8941 aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgcggcc aatgagagcg
     9001 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
     9061 tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc ccggaaaccg
     9121 aagcggtttc tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc
     9181 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggtcgtg
     9241 caggaatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
     9301 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc gacgcgttaa
     9361 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
     9421 ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc acccataacg
     9481 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc
     9541 gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcacg ggtgaaacca
     9601 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
     9661 atatgctggg cgaagctgca ctgactgccg cagacgcaca ggcgtatatg gtttcctatc
     9721 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
     9781 gcatttcaat caaactgtcg gcgctgcatc cgcgctacag ccgcgcccag tatgaccggg
     9841 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtatgata
     9901 ttggtattaa catcgatgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
     9961 aaaatctctg tttcgagccg gaactggcag gctggaacgg catcggtttt gttattcagg
    10021 cttatcaaaa acgctgcccg ttggtgatcg attacctggt tgatctcgcc acccgcagcc
    10081 gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg
    10141 cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt
    10201 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    10261 cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc
    10321 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtac gagcaggtca
    10381 ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg tatttatgcc ccggttggca
    10441 cacatgaaac gctgctggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct
    10501 cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg
    10561 tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa
    10621 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    10681 ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct caatagtgca ctgcaaaaat
    10741 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    10801 accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgcca cgtgaagtag
    10861 aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccacg cctccggctg
    10921 aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga
    10981 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg
    11041 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    11101 cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt
    11161 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag
    11221 aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtacccc
    11281 ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg
    11341 atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    11401 gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    11461 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtcgtggatg
    11521 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcactgcgc gtgctgtgcc
    11581 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggtgcaatg gccgaatgcc
    11641 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    11701 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    11761 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    11821 cgctgatcga actggatgac tttgccgaat tgcacaaaga ggtctttggt ccggtgctgc
    11881 atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg
    11941 gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg
    12001 gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttggtg
    12061 tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    12121 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc
    12181 aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg
    12241 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    12301 agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct
    12361 ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga
    12421 ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc
    12481 atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag
    12541 cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc
    12601 ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt
    12661 ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg
    12721 tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa acaggtagcc
    12781 ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc
    12841 cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc
    12901 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa
    12961 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    13021 gcagatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc
    13081 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact
    13141 tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac
    13201 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    13261 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc
    13321 tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    13381 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    13441 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    13501 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    13561 gtttagtcca ctttttatca gatattttag ataacgtaat attaacatat taataacatt
    13621 aactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    13681 gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    13741 aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca
    13801 gtacatgccg acctttgaac tgaataaagc cattgtgcag aaagcggagc attaccattt
    13861 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    13921 tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct cgcgcattca
    13981 gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    14041 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    14101 gcccgtgtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    14161 ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgat
//
LOCUS       DAJVXP010000108        14158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVXP010000108 DAJVXP010000000
VERSION     DAJVXP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.108"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..228)
                     /locus_tag="OQS10_003041"
     CDS             complement(<1..228)
                     /locus_tag="OQS10_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCS2294004.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OQS10_003042"
     CDS             385..561
                     /locus_tag="OQS10_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCS2294005.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OQS10_003043"
     CDS             1187..1486
                     /locus_tag="OQS10_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294006.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQS10_003044"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OQS10_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS2294007.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OQS10_003045"
     CDS             complement(2472..2939)
                     /locus_tag="OQS10_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS2294008.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OQS10_003046"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OQS10_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS2294009.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OQS10_003047"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OQS10_003047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS2294010.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OQS10_003048"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OQS10_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS2294011.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQS10_003049"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OQS10_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS2294012.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OQS10_003050"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OQS10_003050"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS2294013.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OQS10_003051"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OQS10_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS2294014.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OQS10_003052"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OQS10_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS2294015.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQS10_003053"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OQS10_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS2294016.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OQS10_003054"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OQS10_003054"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS2294017.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="OQS10_003055"
     CDS             complement(13837..14082)
                     /locus_tag="OQS10_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS2294018.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3424 c         3689 g         3599 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaaca cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgt
//
LOCUS       DAJVXP010000109        14108 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVXP010000109 DAJVXP010000000
VERSION     DAJVXP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.109"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(14..1372)
                     /gene="hprS"
                     /locus_tag="OQS10_003056"
     CDS             complement(14..1372)
                     /gene="hprS"
                     /locus_tag="OQS10_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS2294019.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1372..2043)
                     /gene="hprR"
                     /locus_tag="OQS10_003057"
     CDS             complement(1372..2043)
                     /gene="hprR"
                     /locus_tag="OQS10_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS2294020.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2176..2589
                     /gene="hiuH"
                     /locus_tag="OQS10_003058"
     CDS             2176..2589
                     /gene="hiuH"
                     /locus_tag="OQS10_003058"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS2294021.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2698..3702
                     /gene="msrP"
                     /locus_tag="OQS10_003059"
     CDS             2698..3702
                     /gene="msrP"
                     /locus_tag="OQS10_003059"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS2294022.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3703..4338
                     /gene="msrQ"
                     /locus_tag="OQS10_003060"
     CDS             3703..4338
                     /gene="msrQ"
                     /locus_tag="OQS10_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS2294023.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4595..5245
                     /gene="zinT"
                     /locus_tag="OQS10_003061"
     CDS             4595..5245
                     /gene="zinT"
                     /locus_tag="OQS10_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS2294024.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5588..6118
                     /locus_tag="OQS10_003062"
                     /pseudo
     CDS             5588..6118
                     /locus_tag="OQS10_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6688..6777)
                     /locus_tag="OQS10_003063"
     tRNA            complement(6688..6777)
                     /locus_tag="OQS10_003063"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6741..6743),aa:Ser,seq:cga)
     gene            6871..7668
                     /gene="mtfA"
                     /locus_tag="OQS10_003064"
     CDS             6871..7668
                     /gene="mtfA"
                     /locus_tag="OQS10_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS2294025.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7769..7844
                     /locus_tag="OQS10_003065"
     tRNA            7769..7844
                     /locus_tag="OQS10_003065"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Asn,seq:gtt)
     gene            8158..14108
                     /gene="yeeJ"
                     /locus_tag="OQS10_003066"
                     /pseudo
     CDS             8158..14108
                     /gene="yeeJ"
                     /locus_tag="OQS10_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3354 c         3171 g         3593 t
ORIGIN      
        1 ctgaagccct atgttaattt ctttgcggta acataatccg gaacacatta tgcttattga
       61 gatagtgata cgtagcactt cccccatgta attcggcaat cgctttgact aaagaaagac
      121 caagtccctg acctacggaa tgacgcgaat tatctccccg ccaaaatcta cggaagagtt
      181 tttcaggctc atgaattttc gttccaggac tggcgacatc aatattaaga tagccgttgg
      241 tatcaagaaa actggttata tgaatacgcg atttttctgg tgaatatcta atggcattaa
      301 caataagatt cgataacatt cgttgcagta aaattttatc cgcaaagatt tgctgattgc
      361 actcgacctt aaagcaaatc tctttctcgt ctgaaaggta ttcaagataa tccaacaaat
      421 tttcgacttc cttattgaga gaaagcgagt ccagtttcac caaaacattg tttttatctg
      481 cccgggcaag aaacagtatg ttctctgtta accgcgaaat attttccagc tcttcaatgt
      541 taccggcaat tgttttttga tattcagcga tacttctggt ttgactgagc gtaacctgat
      601 tctgacccag taatgcatta atgggcgttc taagttcatg agcgagatcg tcagcaaatt
      661 gacttagacg ctcaaaatct ttgactaaag catgatgcat tttattcaac gcctgcccaa
      721 gaggttttag ttctctcggt aatgcgctaa cctcaacagg ctcccggcta tcgttataat
      781 tcagcgcttc cgttacacca ctcaactttt tgatctctcg taatcccgtt ctgattaaca
      841 gcggacttaa tactgagcaa agtacaatgg cgacaatgca aattataatg ctattaattt
      901 tatactgttc aagcatgtta tgtctggctg aagccaattt agccacagta accgttaatg
      961 atggcgaaac ttcatcaata ttaattcgta aagcatcaat ctcagtatca ttaataatgc
     1021 ttctgtaaat gccagctgcg ctgattgtct cactagcagg tatgttattt aacatgtcat
     1081 cactgacatt tgtccggtta acaattttat tgatgccatc accatgaatg atcaagatat
     1141 cctgactaac atccatcatc cggttaaagt atacaggtaa cgtatctgga tttaccccat
     1201 caattaacaa ctgcttgatc tgtgctgtcc ggttaatgag tgttgtatca tcgcgccatt
     1261 tcaactcact tgccaggcca ttatagagag tccagacaat tccagcgcca gcaacagaca
     1321 gcagcaatat aaaaagcaag gttaaacgga cggttataga taatcttttc attatttttt
     1381 taccgctacg aatgaatagc ccatcccccg gattgtggcg attagctttt caggaaaagg
     1441 atcatcaact tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg tatcactatc
     1501 aaagttgatt ccccaaattt cactcgcaat aaccgttctg ggtataattt cgccagctct
     1561 ggaggccagt agccaaagta actgaaactc cttgcgcgtc agtgtaatac tgatattgtt
     1621 cctgctcaca ctttgactta cagagtccat tcttaagcca ctgatttcta atgttgaatt
     1681 caaagcgtga tgttgcctta attgtgcccg aacccttgcc agcaactcag aaaatgaaaa
     1741 aggttttacc agataatcat ttgctccact gtccagccct ctgactctgt catcgacaga
     1801 atcccttgca gtaaggcaaa taacaggggt ttgctttgct gttcttaacg tttgtaagat
     1861 ctgccagcca tccatacccg gaagcataat atccagaatg atcaatgcat aatcatcctt
     1921 cagcgcaaga taaagcccat ctctgccatc agaaacggca tcaatgacat aacccgcttc
     1981 ggaaagcccc tgcgttaccc actcctgggt cctttgatta tcttcaataa gtaaaatctt
     2041 catgaaaata atatgccata aatcatatgt tatagaaaca gcctagttca ttacaaaatt
     2101 gtaatgctgc tgtaaggtta ccctggccgc tttttcgcta tcctcaaaac tcattcacat
     2161 gacaaggata taaacatgtt aaagcgttat ttagtactct ccgtagtaac ggcagcattt
     2221 tcattacctt ctttggttta tgccgcacaa caaaacattc ttagcgtgca cattttgaac
     2281 cagcaaaccg gaaaacccgc tgccgacgtg acagtcactc ttgaaaagaa agcggacaac
     2341 ggctggttac aacttaatac cgccaaaaca gataaggatg gacgaattaa ggcactgtgg
     2401 cccgagcaaa ctgcaactac gggcgattac cgtgtcgtat ttaaaaccgg ggactatttc
     2461 aagaaacaaa atcttgaaag tttcttccct gagatccccg ttgaatttca tattaataaa
     2521 gtgaacgagc attatcatgt gcctttatta cttagccaat atgggtattc aacctatcgt
     2581 ggcagttaat gcataaatat gtaaaagcag atctctgcta ctgttaggga ggacactccc
     2641 tgacagatta acagtaaacg gctcttgctg gctaacgaca aaaaagtgtg atggcttatg
     2701 aaaaagaatc aatttttaaa agaatcagat gttacggccg agtcggtatt ctttatgaag
     2761 cgtcgacagg tgttaaaagc actgggcatc agcgcagctg cactttcttt gcctcacgct
     2821 gcgcatgccg atctgcttag ctggtttaaa gggaacgatc gcccgcccgc ccccgccgga
     2881 aaaccgctgg agttcagcaa gcctaccgcc tggcaaaata atctgccact gacgccagta
     2941 gataaagtct ccggttataa caacttctat gaattcgggc tggataaagc cgatcccgcc
     3001 gctaatgctg gtagcctgaa aaccgatcca tggacactga aaatcagcgg cgaagtggca
     3061 aaaccattga ccctcgatca cgatgattta acccgtcgct tcccgctgga agagcgtatt
     3121 tatcgtatgc gctgcgtgga ggcatggtcg atggtggtgc cgtggattgg ttttccgctg
     3181 cacaaattgc tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc tttcgaaaca
     3241 atttatgcac cggaacaaat gccaggccag caggaccgct ttatcggcgg cgggctgaaa
     3301 tatccttatg tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac actgatgacc
     3361 gtgggtgttt atggcaaggc gttaccgcca caaaatggcg cgccggtacg actgattgtg
     3421 ccgtggaaat atggctttaa agggattaaa tcgatagtca gtattaagct gacccgcgag
     3481 cgtccgccaa ccacctggaa tctggcagcg cctgacgaat acggttttta cgccaacgtt
     3541 aatccgcatg ttgatcaccc gcgctggtca caggctaccg aacgatttat tggttcaggc
     3601 ggcatcctcg atgttcagcg ccagccaacg ctactgttta atggttacgc cgaccaggtg
     3661 gcatcgctgt atcgtggcct ggatttgcgg gagaatttct gaatgcgtct gacagcaaaa
     3721 caggtgacat ggctgaaagt tagcctgcat cttgccggat tgttgccgtt tctctggctg
     3781 gtctgggcga tcaatcacgg tggactgggt gccgatccgg tgaaagatat tcagcatttt
     3841 accggtcgca ctgcactgaa atttttgctg gcgaccttgt taatcacacc tctggcacgc
     3901 tacgcaaaac agccgttatt gatacgcact cgccgcctgt taggcttatg gtgctttgcc
     3961 tgggcgacac tgcatttaac cagttacgca ttgctggagc tgggcgtgaa taatctggcg
     4021 ttattaggca aggagttaat tacccggcct tatttaacgt taggaattat cagctgggtt
     4081 attttgcttg ctttagcgtt tacttcaacc caggcgatgc agcgaaaact cggcaagcac
     4141 tggcaacagt tgcataattt cgtctatctt gtcgcgatcc tggccccaat acattatctg
     4201 tggtcagtga agattatttc accgcagccg ctcatctacg ctgggctggc tgtactgctt
     4261 ttagccttac ggtataagaa gttgcgttcc ctttttaagc ggttacgcaa gcaggttcac
     4321 aacaaattat ctgtataact tcatattgcc gacaaagtac gcgttatccc tgccggatgt
     4381 ggcgcgagcg ccctgtccgg cctgcgcaga tcacatcagg ttatgtttcg tttgtcatca
     4441 acctcaaatt atcaacataa ttcattttat ttcaataaga tagataagta cacctgagaa
     4501 aactatgctc tcatctccta agagttgttg cattttgcca tatgttacaa tataacatta
     4561 cacatcatat acaatgactc tggaggaaac tgttttggca attcgtcttc acaaactggc
     4621 tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcactcaca
     4681 cggcaaaccc ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgatgccaa
     4741 tgtacaaaac cgaacgctca gtgactggga tggagtctgg caatccgttt atcctttact
     4801 gcaaagtggc aaacttgacc ccgtctttca gaagaaagcg gatgcagata aaattaaaac
     4861 atttgctgaa attaaagatt attatcacaa aggttatgca acagatatcg agatgattgg
     4921 cattgaggac ggcattgttg aattccatag aaataatgaa acaacatcct gtaaatatga
     4981 ttacgatgga tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt
     5041 cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat
     5101 tgcaccacga aaatcatctc attttcacat ttttatgggt aatgattcac agcaattttt
     5161 attaaatgaa atggaaaact ggccaacgta ttatccatat cagttgagta gcgaagaagt
     5221 ggtcgaggaa atgatgtctc attgagtatt ctcatgataa cgcctcgatg ccgctttagt
     5281 aagtcatcat aactgccact ggtccatcca caaacgccac tgaacgcaag ctagctacag
     5341 acacgctcat cactatgacg tgtctgtata ttaataagct aacccgcatt gagttaacca
     5401 ataacggatt ccatacacaa tacggccaac agagaaagat acctgtgctc acgccattgc
     5461 ttatattggc tggttacaat gtgcactatc aattttttaa ataacaaatt cttaaaacca
     5521 catctgtata aggaattttt aaggttcgtg ggatagcttg actgtaaaaa ttacaggagc
     5581 tacaaaaatg aaccgattct caaaaactca aatttattta cattggataa cgctgctttt
     5641 cgttgcaata acctatgccg cgatggaact ccgtggctgg tttcctaaag gtagtagtac
     5701 ttatctgctg atgcgagaaa cacattacaa tgcgggtata ttcgtttggg tgttaatgtt
     5761 ttcacgcctg attataaaac accgttatag tgatccttct attgtaccac cgccacctgc
     5821 ctggcaaatg aaagcggctt cgctaatgca catcatgctt tatataacct tccttgcact
     5881 tcctctgctg gggattgctt tgatggctta cagtggaaaa tcgtggagtt tccttggttt
     5941 caatgtgtct ccctttgtta ccccaaacag cgaaattaaa gcactgataa aaaatattca
     6001 cgaaacctag gcaaatatag gttacttttt aatcgcagct cacgctggcg cagcactctt
     6061 tcatcactac attcagaaag ataatactct gttacgaatg atgcatcgcc gcaaataagt
     6121 cgatcccctg acaaggaaat gtgaaccatg tacatcaacg cgtcataaat gtttacgcaa
     6181 gtgcacaatg catcctttct actatcaaca aggaggagca gaaactgtgt aaatcgtaca
     6241 atggtaagac ttatcaaaaa tacaggttaa ttttggtatt taaacctttt ttacttgtta
     6301 gtgtgataca aaggctttca aaaaagctgg gaaattcaac aactcttgga aattatggac
     6361 tttgtcccgc ggctttaatc tgacggccgc gttccttttt tgactcaatt attgacccct
     6421 tctctacgca acttcagttc ccaccaccaa ctttgcggca gtttcatagg atgaatgtct
     6481 aaaagaataa tggtgagcga taagaaaacg actgaataac tgcacatttt cgctcgaaag
     6541 cttcctgtca gatccatagc gaatcaagtg ctgaatgtca cagtatcgaa cagaaaacag
     6601 tgacgatcta acccttcaag aatattctac aattgttctg tttaggaaaa gcaaggcggg
     6661 aagtcaggag ataagtcatt gataaagtgg cggagagagg gggatttgaa cccccggtag
     6721 agttgcccct actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt
     6781 cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtt cgtgcaattt
     6841 tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa
     6901 tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct tttaacgggt
     6961 ctgacggaac aggaacaaag caaattagtc actcttgccg aacgtttttt acagcaaaag
     7021 cggcttgttc ctttacaggg ctttgaactg gattcattaa gaagctgccg gatagcactt
     7081 ctattttgcc tacccgttct ggagttagga ctggaatggc tggatggttt tcatgaagtc
     7141 ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg
     7201 cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac
     7261 tggttggata tacaagattc ttttgatgca tctggtttta acctgattat tcatgaagtc
     7321 gctcataagc tggacacccg taacggcgat cgcgccagcg gagttccctt tattccgttg
     7381 cgtgaggttg ctggctggga acacgatctt catgctgcaa tgaacaacat tcaggaagaa
     7441 atcgaattgg ttggcgagaa tgcggcgagc attgatgctt atgcagccag cgatcctgct
     7501 gaatgttttg ccgtactttc tgaatatttc tttagcgccc cagaactttt tgctcctcgt
     7561 ttcccttcat tgtggcaacg tttctgccaa ttttatcaac aagatccttt gcagagactg
     7621 catcacgcta atgatacaga ctcgttttcg gcgacgaatg ttcattaatt aacaactttg
     7681 cagattaatt aaccaattga aatgacttat gaaatttagt gttgacagac aaggtaccgc
     7741 taagtaatat gtgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     7801 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattct aaaaattcgc
     7861 ttttttagcg caatgtcact gaccttagtt gaacattgtt ttttaacgga tagcgggttt
     7921 ttaacacctt aagcgccctc gacctttatg gttgagggcg ttttgctatg aacgccatca
     7981 ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct tgcaactcac
     8041 acccaaaact gcctaacgaa aagttattaa ttttcaatcg tattgctatc agtatttaca
     8101 ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa ggtaagcatg
     8161 gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatattatg cgggatggga
     8221 attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc gttccctatg
     8281 gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc tgcacaaatt
     8341 gccattgcaa atgccaatac ggtgccctac acccttggag cgctggaatc ggcccaaagc
     8401 gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca gtttcgtacg
     8461 tttgctcgag gttttgataa tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt
     8521 agtgaaaaaa aattaacccc gccgccgggt aatagcagtg acaacctcga gcaacagata
     8581 gccagtactt cacagcaaat cgggtctctg ctcgccgaag atatgaacag cgagcaagcg
     8641 gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac agactggtta
     8701 agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag cctgaagaac
     8761 tcccagttcg attttctcca tccgtggtat gaaacgcctg ataatctctt tttcagtcag
     8821 catactctcc atcgtactga cgagcgtacg cagattaaca acggcttagg ttggcgtcat
     8881 ttcactccca catggatgtc gggcatcaac ttctttttcg accacgatct tagccgttac
     8941 cactcccgcg tcggcattgg cgcggagtac tggcgcgact atctaaaatt aagcagtaac
     9001 ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga ttataaagca
     9061 cgcccggcca atggctggga tgtacgcgca gaaagctggc tacccgcctg gccgcacctt
     9121 ggcggtaaac tggtctatga acagtattat ggcgattaag tggccctgtt cgataaagac
     9181 gatcggcaaa gtaatcctca tgccataacc gctggactta actatacccc cttcccgctg
     9241 atgaccttca gcgcggagca acgccagggt aaacagggcg aaaatgacac ccgttttgcc
     9301 gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc gaatgaagtc
     9361 gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa caacaatatc
     9421 gttctggaat atcgcaaaaa agaactggtt cgcctaaccc tgacagaccc cgtgacaggg
     9481 aagtcaggag aagtgaaatc actggtttcg tcgctacaaa ccaaatatgc cctgaaaggc
     9541 tataacgtcg aagccaccgc actggaagct gccggtggca aagtggtcac aacgggtaaa
     9601 gatattctag ttaccctgcc ggcttaccgg ttcaccagta cgccagaaac cgataacacc
     9661 tggccgattg aagtcaccgc cgaagatgtc aaaggcaatt tgtcgaatcg tgaacagagc
     9721 atggtggtcg ttcaggcacc tacgctaagc cagaaagatt cctcggtatc gttaagtacc
     9781 caaacattga acgcggattc ccattcaacc gccacactga cttttattgc gcatgatgca
     9841 gcaggtaatc ctgttgtcgg gctggtgctc tcgacgcgtc acgaaggtgt tcaggacatc
     9901 accctttctg actggaaaga taatggtgac ggaagctata cccagatcct gaccacagga
     9961 gcgatgtctg gcacgctgac gctgatgcca cagctgaatg gtgtggatgc ggctaaagcc
    10021 cccgccgtgg tgaatatcat ttctgtttcg tcatcccgaa ctcactcgtc aattaagatt
    10081 gataaggacc gttatctctc cggcaatcct atcgaggtga cggtagaact gagagatgaa
    10141 aatgacaaac ctgttaagga acaaaaacag caactgaata acgcagtcag catcgacaac
    10201 gtgaaaccag gagtcactac agactggaaa gaaaccgcag atggcgtcta taaggcgacc
    10261 tataccgcct ataccaaagg cagtggactt actgcgaagc tattggaatg aagatttgca
    10321 taccgctgga tttatcatcg acgccaaccc gcagtcagcg aaaattgcga cattatctgc
    10381 cagcaataat ggtgtactcg ccaatgagaa tgcagcaaac accgtctcgg tcaatgtcgc
    10441 tgatgaagga agcaacccaa tcaatgatca taccgtcacg tttgcggtat taagcggatc
    10501 ggcaacttcc ttcaacaatc aaaacaccgc aaaaacggat gttaatggtc tggcgacttt
    10561 tgatctgaaa agtagtaagc aggaagacaa cacggttgaa gtcacccttg aaaatggcgt
    10621 gaaacaaacg ttaatcgtca gttttgtcgg cgactcgagt accgcgcagg ttgatctgca
    10681 gaagtcgaaa aatgaagtgg ttgctgacgg caatgacagc gccacaatga ccgcgaccgt
    10741 ccgggatgca aaaggcaacc tgctcaatga cgtcatggtc actttcaatg ttaattcagc
    10801 agaggcgaaa ctgagccaaa ccgaagtgaa tagccacgac gggatcgcca cagctacgct
    10861 gaccagtttg aaaaatggtg attatagggt tacggcctct gtgagctctg gttcccaggc
    10921 taatcaacag gtgaatttta tcggtgatca aagtactgct gccctgaccc tcagtgtgcc
    10981 ttcaggtgat atcaccgtca ccaacacagc tccgcaacat atgactgcaa ccttgcagga
    11041 taaaaatggc aacccactaa aagataaaga aatcaccttc tctgtgccaa acgacgtcgc
    11101 aagtaagttc tcgattagca acggaggaaa aggcatgacg gatagtaacg gggttgcaat
    11161 cgcctccctg accggcacgt tagcgggcac gcatatgatc atggctcgtc tggctaacag
    11221 caatgtcagc gatgcacagc caatgacgtt tgtggcggat aaagacagag cggttgtcgt
    11281 tttgcaaaca tcgaaagcgg aaatcattgg gaatggcgtg gatgagacaa ctctgacagc
    11341 aacagtgaaa gatccgtcga atcatccggt ggcggggata acggtaaact tcaccatgcc
    11401 acaggacgtt gcggcaaact ttacccttga aaataacggt attgccatca ctcaggccaa
    11461 tggggaagcg catgtcacgc tgaaaggtaa aaaagcgggc acgcatacgg ttaccgcaac
    11521 gctgggtaat aacaatacca gtgattcgca gccggtaaca tttgtggcgg acaaagcctc
    11581 ggctcaggtt gtcctgcaga tatcaaaaga tgagatcaca ggtaatggcg tcgatagcgc
    11641 aacgctaact gcaacggtta aagatcagtt cgacaatgag gtgaataatc ttccggtaac
    11701 attcagctca gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc
    11761 tggcatcgcg caggccactc tcgcaggcgt tgcctttggt gagcagacgg tcactgcatc
    11821 actggctaat aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc
    11881 ggcaaaaatt atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa
    11941 cagctccggc agcgccatca ccgccacagt cgttgataat aatggctttc cggtgaaagg
    12001 tgtgactgtg aacttcacca gcagaacaaa ctctgccgaa atgacgaatg gcggccaagc
    12061 cgtaacgaac gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga
    12121 atcaggagcg agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag
    12181 cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat ctcacctggc tacaggcact
    12241 ttttgataca gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta
    12301 cggcaacggt gtcccccagc atcaggtgac cctcagcgtt tcaccaagtg aaggcgtgac
    12361 ccccagtaat aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac
    12421 cgctacaaaa gccggggttt atcaagtgac ggcaaccctc gaaaatggcg attcgatgca
    12481 acaaacagtg acctatgtgc cgaacgtcac gaatgccgaa atcacgctgg caacctcgaa
    12541 ggatccggtg attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac
    12601 agagggcaat gcgatagcca acactgaagt aacatttact ctgccggaag atgttaaggc
    12661 gaacttcacg ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt
    12721 cacgctgaaa ggtacaaaag caggcgctca tactgttaca gcatcgataa ctggcggtaa
    12781 gagtgagcag ttggtggtga actttactgc ggatacgctc actgcgcagg ttaatcttaa
    12841 cgttaccgag gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt
    12901 gactgatgga aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga
    12961 tgtgagcgca agctttactc tcggacaagg cggttccgcc attactgaca tcaacggcaa
    13021 ggctgaagtt acactgagcg gtacaaaatc cggcacctac cccgtgacag ttagcgtgaa
    13081 caattatggt gtcagtgata cgaaacaggt gactttgatt gccgatgctg gtaccgcaac
    13141 actagcctcc ttaacctctg tatactcatt cgtcgtcagc acgaccgaag gcgcgaccat
    13201 gactgcaagc gtcactgacg ctaacggcaa cccggtagaa ggcataaaag ttaatttccg
    13261 cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg gatgatcggg gtttcactga
    13321 aattcttgtg acaagcaccg aagtcggact gaaaacagtt tcagcctctc tggcagataa
    13381 acctactgaa gtcatatcgc gattactgaa tgcaaaagca gatattaatt ctgcaacgat
    13441 taccagtctg gagatacctg aaggtcaggt catggtcgca caagacgtag cagttaaagc
    13501 tcacgtcaac gaccagtttg gcaacccgat tcttaatgaa tctgtaacat tcagtgcaga
    13561 gccaccagag cacatgacca tcagccaaaa tattgtctct actgatacgc atggtatagc
    13621 cgaggtctcc atgacgcccg aaagaaacgg ttcgtatatg gtgaaagcat ccctggcgaa
    13681 tggagcctca cttgagaaac aactggaggc tattgatgaa aaactgacac tctcggcgtc
    13741 cagcccgctt atcggtgtca attccccaac aggtgcaact ctgacggcaa cgctaacttc
    13801 tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc cagaaggtgc
    13861 gacgttaagt ggcggaaaag tgagaactaa ctcttctggt caggctccag tcgttctgac
    13921 cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggcg taacaataca
    13981 gacacagaca accgtgaaag tcactggcaa ctcaagcacc gcccatgtta ctagctttat
    14041 cgctgatcca tcgactatcg ccgccaccaa cagtgattta agtaccttaa aggcaacggt
    14101 tgaggatg
//
LOCUS       DAJVXP010000110        13964 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVXP010000110 DAJVXP010000000
VERSION     DAJVXP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.110"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OQS10_003067"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OQS10_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS2294026.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OQS10_003068"
     CDS             2273..2662
                     /locus_tag="OQS10_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS2294027.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OQS10_003069"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OQS10_003069"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS2294028.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQS10_003070"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OQS10_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS2294029.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OQS10_003071"
     CDS             complement(4321..4521)
                     /locus_tag="OQS10_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS2294030.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OQS10_003072"
     CDS             4687..5232
                     /locus_tag="OQS10_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS2294031.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OQS10_003073"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OQS10_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OQS10_003074"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OQS10_003074"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS2294032.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OQS10_003075"
     CDS             complement(6529..7353)
                     /locus_tag="OQS10_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS2294033.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQS10_003076"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OQS10_003076"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS2294034.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQS10_003077"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OQS10_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS2294035.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQS10_003078"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OQS10_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS2294036.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQS10_003079"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OQS10_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS2294037.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQS10_003080"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OQS10_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS2294038.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQS10_003081"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OQS10_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS2294039.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OQS10_003082"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OQS10_003082"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS2294040.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3390 a         3535 c         3712 g         3327 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DAJVXP010000111        13903 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVXP010000111 DAJVXP010000000
VERSION     DAJVXP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.111"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="OQS10_003083"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="OQS10_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS2294041.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="OQS10_003084"
     CDS             complement(1550..1723)
                     /locus_tag="OQS10_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS2294042.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="OQS10_003085"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="OQS10_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS2294043.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="OQS10_003086"
     CDS             3361..3690
                     /locus_tag="OQS10_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS2294044.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OQS10_003087"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OQS10_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS2294045.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OQS10_003088"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OQS10_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS2294046.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OQS10_003089"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OQS10_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS2294047.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OQS10_003090"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OQS10_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS2294048.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OQS10_003091"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OQS10_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS2294049.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="OQS10_003092"
     CDS             9613..9909
                     /locus_tag="OQS10_003092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294050.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OQS10_003093"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OQS10_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS2294051.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="OQS10_003094"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="OQS10_003094"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS2294052.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3438 c         3230 g         3577 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgc tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJVXP010000112        13838 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVXP010000112 DAJVXP010000000
VERSION     DAJVXP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.112"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(30..788)
                     /gene="dsbG"
                     /locus_tag="OQS10_003095"
     CDS             complement(30..788)
                     /gene="dsbG"
                     /locus_tag="OQS10_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS2294053.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1160..1723
                     /gene="ahpC"
                     /locus_tag="OQS10_003096"
     CDS             1160..1723
                     /gene="ahpC"
                     /locus_tag="OQS10_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS2294054.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1894..3459
                     /gene="ahpF"
                     /locus_tag="OQS10_003097"
     CDS             1894..3459
                     /gene="ahpF"
                     /locus_tag="OQS10_003097"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS2294055.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3580..4008)
                     /gene="uspG"
                     /locus_tag="OQS10_003098"
     CDS             complement(3580..4008)
                     /gene="uspG"
                     /locus_tag="OQS10_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS2294056.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4229..5467
                     /locus_tag="OQS10_003099"
     CDS             4229..5467
                     /locus_tag="OQS10_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS2294057.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5698..6108)
                     /gene="rnk"
                     /locus_tag="OQS10_003100"
     CDS             complement(5698..6108)
                     /gene="rnk"
                     /locus_tag="OQS10_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS2294058.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6338..7144)
                     /gene="rna"
                     /locus_tag="OQS10_003101"
     CDS             complement(6338..7144)
                     /gene="rna"
                     /locus_tag="OQS10_003101"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS2294059.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7258..8721)
                     /gene="citT"
                     /locus_tag="OQS10_003102"
     CDS             complement(7258..8721)
                     /gene="citT"
                     /locus_tag="OQS10_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS2294060.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8772..9650)
                     /gene="citG"
                     /locus_tag="OQS10_003103"
     CDS             complement(8772..9650)
                     /gene="citG"
                     /locus_tag="OQS10_003103"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS2294061.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9625..10176)
                     /gene="citX"
                     /locus_tag="OQS10_003104"
     CDS             complement(9625..10176)
                     /gene="citX"
                     /locus_tag="OQS10_003104"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS2294062.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10180..11712)
                     /gene="citF"
                     /locus_tag="OQS10_003105"
     CDS             complement(10180..11712)
                     /gene="citF"
                     /locus_tag="OQS10_003105"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS2294063.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11723..12631)
                     /gene="citE"
                     /locus_tag="OQS10_003106"
     CDS             complement(11723..12631)
                     /gene="citE"
                     /locus_tag="OQS10_003106"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS2294064.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12628..12924)
                     /gene="citD"
                     /locus_tag="OQS10_003107"
     CDS             complement(12628..12924)
                     /gene="citD"
                     /locus_tag="OQS10_003107"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS2294065.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12939..>13838)
                     /gene="citC"
                     /locus_tag="OQS10_003108"
     CDS             complement(12939..>13838)
                     /gene="citC"
                     /locus_tag="OQS10_003108"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS2294066.1"
                     /translation="IAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYT
                     KTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNAN
                     PFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSE
                     YIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQY
                     NQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVT
                     LHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3402 a         3504 c         3678 g         3254 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac ccccataatg
       61 atattaagcg ttttctgatc gggcaacccc acggcctgtt gcagcgtatt ttccttactc
      121 atgtaataga tagccggcgt gacatttgcc cccagatcgt ccatcagttt ctcattgtca
      181 cttaacactt tcatttgctc tgtacttacg tttgcaggca cgtttagctt aagcttgcca
      241 ccagaggctt catattgttg ccaggttttt gcgggatctt tggaggcaag aattgccgct
      301 gctgtcgccg ggctttctgg cttgataacc ccaaccaaca atgttcttaa ttgcactttg
      361 ccagaatcta cccacgggcg cgcctgctgc cagaactgtt tacaatatgg gcagaacgga
      421 tcggcgaaga cgtagacaat gaccggcgca tcttttttac cgtcgaggat ccagtgggat
      481 tgttccatcc gttgccacat ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt
      541 gtgttactca ggttttcacc tttctcgttg tacatgtaac cagagatagc gtgcttacca
      601 tcgggagtca ggtagatggt gacgcccata tcctgatact ttccgagata acctttcatt
      661 cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat cgcttttact
      721 ggggaaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa aagtatcttt
      781 tttaacatta atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc
      841 actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg gtgttgtaat
      901 ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc gctgaagata
      961 ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac tttttatcgc
     1021 cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga
     1081 tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac atctcatcga
     1141 aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt ttaaaaacca
     1201 ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag gccgctggag
     1261 cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt
     1321 tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag tatctaccga
     1381 tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta aaatcaaata
     1441 tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga
     1501 tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca tccaggcaat
     1561 cgaagttacc gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta aaatcaaagc
     1621 agctcagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga aagaaggtga
     1681 agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct tcgtctttcg
     1741 cgctaccagc tccggcgcga aatacttcgg aaattcacct aattcttcgg gtgctgcggc
     1801 acccgatttt cttccccgca ccatgatgca agctgcatcc aggtagccgt agaggccgct
     1861 tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat gaaaactcaa
     1921 ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac gctggatgac
     1981 agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact gtcagacaaa
     2041 gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct gatcaccaac
     2101 ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga gttcacctcg
     2161 ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca gtctctgctg
     2221 gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc gctctcttgc
     2281 cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc
     2341 aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg
     2401 ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat gacgttgact
     2461 gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga gctgaacaag
     2521 cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc ggcagcaatt
     2581 tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc
     2641 ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg gcagaagctg
     2701 gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga cagccagagc
     2761 gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga aacagcttct
     2821 ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg gcgcaacatg
     2881 aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc gcactgcgac
     2941 ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa
     3001 gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa
     3061 atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt cgacattatt
     3121 ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg tctggaatat
     3181 cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt cgtgcagatt
     3241 ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg catgggtgag
     3301 attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc gggtgactgt
     3361 acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa agcctctttg
     3421 agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag attcacctgc
     3481 aattgcttag ccgccggggt caaacctggc ggctttttta tggcattaaa aagccctgcc
     3541 gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaatga ccagtacagg
     3601 cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg tcgaaatcga
     3661 tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca actcattgac
     3721 ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat cgatggtgaa
     3781 gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct cttcaaaacg
     3841 acgcacatca gcggcaaaac ggtgcaggct caggctggct gacccgggta gtacgtgaag
     3901 tagatgaata actccgtcat cctgggcgag gaattcagcg tggcgaacag ctttgtcgct
     3961 caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa ccctttctcc
     4021 ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga tatttgtctg
     4081 gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac accaggaatt
     4141 ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt aaagactgac
     4201 tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg gcccacatca
     4261 cgttcaggta gaaaatgttc ccgatccggg cattgaacag gctgatgata ttattctgcg
     4321 tattacggca acggcgatct gtggctctga cctccatctt tatcgaggca aaatacccca
     4381 ggttaaacat ggcgatattt tgggtcatga atttatgggg gaagtcgttg aaaccggaaa
     4441 ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca ttgcttgtgg
     4501 cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca atgcgggtaa
     4561 aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg gttatagtca
     4621 cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta aagggaatgt
     4681 ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc tttctgatat
     4741 tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt caagcgttgc
     4801 agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt tgctcggtgc
     4861 ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg ctgaccgcta
     4921 cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa ttattgaaca
     4981 aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg aagcgaaagg
     5041 cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg gtaaagcgtt
     5101 gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg gcgtctacgc
     5161 tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga cgtttaaaat
     5221 gggacagacc cacgttcacg catggctggg tgaattatta ccgttaattg agaaaggatt
     5281 actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg cccggggata
     5341 tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac ccggtgcaca
     5401 aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc cggggggaac
     5461 aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata atcgccgcca
     5521 taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc acaaacgaaa
     5581 cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct gattttgcgt
     5641 aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca gatgggatta
     5701 aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt caaggtgggt
     5761 tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc ccagcagtgc
     5821 ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt ttgccggata
     5881 caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa cccggctgtt
     5941 cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc gatccaactc
     6001 tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca gcagaatatc
     6061 gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt ttactccatg
     6121 tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc accgtcaggc
     6181 ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa gtgatttgcg
     6241 tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc gtcacggttt
     6301 atggaagacg aattagcgtg ttttgtaaat cagatgatta ataacccact ttatcaatca
     6361 caaagttttt gccacagttg cctgggtgtg gttgtggcaa gaatgagttt gcagaaagcg
     6421 gagcgttgat ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac gcagggttac
     6481 cctggcacgt tagcttaact gctttcacgt tctcttttcc ccagcttttg gcaaaggcgg
     6541 cgtcaaagtc acggcggctc actgttttac cgtaattatc cgcaaggaat tttccggctt
     6601 cgctctcttt aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg tccggatcaa
     6661 aaccaaagca ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa cgtccgccag
     6721 ctcctggcat gacttcactt agtttagcgg ccgtttccag tgataacccg gtttccggcg
     6781 atgaacacat ccggctggcg tgcgcttctg gtagattcgg gattgggcga gtagcgcaac
     6841 cgaagcgcat ccagcggcgt tcatcaacac cacgggcagc aaccgattta ggcaatcctg
     6901 gccacagacc atgtacggtc agaaaatcag ctttgttggt cgtttcggtt tgtaggcgac
     6961 attcatctcg ttcgttacga tttcgatcgt gttgactctg gcaaaatccg gtttgccagg
     7021 agagggccag gacatagcga tcaaaatcgc catactgttt tgcctgcaac gctacggcgt
     7081 tggcagaaga gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc cagaatgctt
     7141 tcataatggg tgtggaactc atacatacac tgaatactat ctattaaatc ataaaaaagc
     7201 ccgccaggtg ggcttacctg gcgggcgtga tgatttattc agcgtttggc gaacgtatta
     7261 gttccacatg gcgagaatcg gccagccaac caacagcaac atagagatgt aaatcacccc
     7321 gaagattgcg ccaagacgcc agtaatcttt tgatttcaca tagccacagc cgtaaataat
     7381 caccccagga ccggttgcat acggcgtcag acagcccatg ataccgatag acagcaccag
     7441 caggatacac agttgttcca ttggtacgcc cggaatacct ttaccgacgg ccagaataac
     7501 cgacagcatg gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg caaagtagaa
     7561 caccagaacc agtacaatca ccgttgcgtt tggtgagaat ccttccaggt gcgtactcat
     7621 ggtaccggcg aaccagtcaa taaagccaga acgagtcagg ccgttagcca tcacaaccag
     7681 agttgccagg ttgaccagcg tgttccatgc gctgttatag cgggtaatat ctttccaagg
     7741 cacaacgtgc agggccagca ttagcgaaac tgccagcaga ccaactgcag tagcattaat
     7801 gacttcactg ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa tcagtgtcca
     7861 ctctctgcgt gtcagcgcac ccatggtttt cagttcatca cccgcccagg ttgccacttc
     7921 ttcactgtgt gtgatttccg gtttgtacag cacgtaggaa agccacggcg caatgataag
     7981 caagataacc ccaaccggca ggaagcagag gaaccactgc aaccagctaa tctggatacc
     8041 ggcaattttg ctgacgaact ccagacccag cacgtttggt gccgcaccgg tgacaaacat
     8101 ggacgaactc agactggtac taatgaccat catccacatc aaatagccgc caatacgacg
     8161 cgcggacgga tcgttgggga atgatttaaa cagcggcggc aggtttttaa tgaccggaaa
     8221 aaccgtaccc ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca gaatgtcgat
     8281 aatgacaatc gcataaccca acgtcagcgt gcgtttgccc atgaatttca ccaggaaaag
     8341 ggcaatgcga cgacctaacc cggaaacttc ataccctaat gcaaaaataa atgcgccaaa
     8401 taccagccat accgtggtgc tggaaaaacc agccagaccc catttcagcg cctgtttttg
     8461 cgcattaaac gctgggtcag ctaattcttt ggcatcaaag agcaggtaat tactgccaat
     8521 aacgcaaata gtaaccgcaa taaaactgat cgctgttgcc ggaattggct cgaggatcat
     8581 gccgacaatc attgccacaa acacagcgaa gtaatgccat gcctgtggtg gcataccgtc
     8641 ggggacaggg ataagaaaca tgacacccat caccaccagt ggggccaata gtttccatat
     8701 attatctttt gctaaagaca tacgggttct ccgaaaatta atatttccaa atttatcaag
     8761 tgcttaaata attaaatctg tgctaaaaac caggtaagga tcagcaggtc ggcactgccg
     8821 cctggactga gatttcgttc gatacactcc ctgtcgaact gccggagata atcgagatcg
     8881 gcgggggttc gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg tagccagcgc
     8941 aggcccccct cgccaccgcg cgatgcaacg ttggtatcgc cgttgatcgc catcagtagg
     9001 agcaaggtat cgagcaatgc cagttcagga tctaacccct gatccagcag agtgaggtaa
     9061 tgcggcaagg cgtgattgat caccagtgga taacccgctt cggcttcacc gcgtgcgccg
     9121 gtaaggccaa gctgctggta caaccgttga cctgccgtca gttgtgaatt attggtacgc
     9181 agttctcgat cggtcaggcc acggcagaaa cttgccgccg tagaacaaac ggttgttggc
     9241 gttaccggtt ggttgagttg aagcaaacgg ccaattgccg cacatagtag ccctaaagaa
     9301 aaaatgctgc ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc accttcgcaa
     9361 gccataccaa ttgggcgtaa tccgtggagt accgcttctg gtgctatttc cgcactacag
     9421 gcaccaaatt caatgaaacg gggtagccag ccctgaatcg ccagcgcgct gcggtggaaa
     9481 tcttccagcg ccatatcttt atgtgcaccg cagttaatcc gatccacgag gcctggtttc
     9541 ggtgacagat tgacttcagt cagcatggcg cgccagccca gcagggcgta ctcatcgatc
     9601 aatgacgtcg caagctttgt ggttttagtt gacgttgcag gcatcgacat cgttcagcag
     9661 tgcctccatg cggttgagta aatcggtcag ttgatgggtt tttccacgcg cgcagacggc
     9721 tgcgctttgc tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc ggcgggagag
     9781 aatttcgcct tcgggcgtca ggacatcgat atcccataac cgcccgagag gatgactatg
     9841 ttcaagctca atggtggcga gcttgaggtc gcgagccggg gcggcaatgc tcaacatgcc
     9901 ctccggcccg ctggcggaaa ccagtgcagc ctgctcctga atttgccagc cctgttttgc
     9961 ggctaaggca cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga cctcgctgtc
    10021 tttaatcggc ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat ggcgcttgag
    10081 ccagacgtgt tgccgtgctt gcctttcatc ccggctgacg agcagctcgg gaattgacac
    10141 cgcatggtgg ctggcgagtt caggaagcag gtgcatggct tattccttca cctgatgcac
    10201 aacatcgatc accgagccat cgcggtaacg cacaacggca acgacgcggt cggtgaattc
    10261 aatcggctgt ggttcaccgg tcagcagacg cgcgcgttcg cgcagccact caatggaaac
    10321 cactttaatg cccgcttcct gcagacgttc tgccagttcc ggacgtgccg ggttaactgc
    10381 gataccgtgg tctgtgacca gaatatcgac actggagcct ggggtgatgc aggtcagcac
    10441 gttatccacc agagtcggaa tacgaccgcg taccagcggc gcgacgatga tggaaagcgc
    10501 agaggcaatc gcggtatcgc agtgaccacc ggaagcacca cgcagtacgc cgtcagagcc
    10561 ggtcagcacg ttaacgttga actgggtgtc aatttccagc gcgctcagta ccaccacgtc
    10621 gagacgatca accgatgcgc ctttcgaacc ccagttagcg tactggttgg cgctgatttc
    10681 gatgtgattg gggttacggg ccagcgattg cgcagcatgg ctgtcaaagc tctgcacatc
    10741 cagcagtttg cggatcagac ctttttcgtg cagatcaacc atcgtcgcgg taataccgcc
    10801 gagggcgaag tcggcgcgaa tatcgcggct acgcattttg tcttccagga aacgggttac
    10861 cgccagcgat gcgccgccgg tgccggtttg catggagaaa ccttctttga agtagccaga
    10921 gttgacaatc acatccgcag cgctacgggc aataagcagt tcgcgcgggt tagtggtcat
    10981 acgggtcgcg ccagcgccga tttttgcagc atcgccaacg cggtcaactt tgacgatcaa
    11041 atcaacctga tcttgctcaa tgcttgccgg attatgcgga taaggcagca gttcttcggt
    11101 aagcatcaca acctgttttg cgttgtcggc atcaaccatt gcatagccga gggagccgca
    11161 gcaggcttta ccggtgtaac cgttggcatt accgaattca tcacaggacg ggacgccgag
    11221 gaaagccacg tcgatattca gttcgccgct ctgtaccaga tgcacacgtc cgccgtgaga
    11281 gtggatttgc accggttccg caagcagacc acgggaaatt tcttctgcca gtggaccgcg
    11341 caggccggag gtataaatgc gggtaaccac gccctggcga atgtgttcta ccagtggcgc
    11401 atggcaatca ctcagggagc tggacgccag ggtcaggttt ttaaagccca tcttagcgat
    11461 gacgtccatc accatattga cggtcaggtc accgccacgg aaagcgtgat ggaaggaaac
    11521 cgtcatgccg tcctgtaaac cagagcgacg aatcgcttct tcctggttgg cgcacagttt
    11581 gcgatcgcgc gctttttcag cctggtaggt ttgctttggt gagttctgga aagcggcaag
    11641 atcgcattca gcgcgacgat tccaggccgc tacccgttct tgtcgttgag attgttcaat
    11701 tttctgcgtc attttgattg ccttattctt cgcggatgcc ggaaagttct gcacgggaga
    11761 gcaccagacg ggcacgatcg ataaccggac cgtccaccat cttgccgttc agggaaacca
    11821 cgccgaggcc ttcgcgagcg gcggcttcag cggcttctac gacgcggcgg gcgtgatcca
    11881 cttctttctg ggtcggtgcg tagaggttgt gcaacagatc aatctgacgc gggttgatca
    11941 gcgatttgcc gtcaaagccc agctgtttga tgtgggcggc ttcttgcagg aatccggctt
    12001 cgttgttagc gtcggaatag acggtatcga acgcctgaat acccgcagag cgcgcggcct
    12061 gcaaaatgga acagcgtgcg aacagcagtt cagttccttc cggggagcgt tctgtacgca
    12121 ggttgcgcac atagtcttct gcaccgaggg cgataccgat caaacgctcg gaagcgtgag
    12181 cgatttccac tgcgcgggta atccccagcg gagattcaat cgccgccagc aggccggtgc
    12241 tgccgggttc acgaccacag gctttttcga tacgcaggat ctctttttcg atatccagaa
    12301 catcctgagc ggtatcggtt ttcggcagac gcacaacgtc cgcaccaccg cgaacgacgg
    12361 cttccaggtc gttgacaccc cattcggaat ccagcgcgtt gacacgcaca atggtttcaa
    12421 tatcgcgata cagcggatgt tgcagcgcgt ggtaaaccat gcggcgggcg gtgtcttttt
    12481 cacgcaatgc tacggagtct tcgaggtcaa acatcagggc atcagccggg tagatgaagg
    12541 agttgctgac catcgcggca ttggcaccag gcacaaacaa catgctgcgg cgggtgcgag
    12601 ttttacgttg ttgcagcgaa gcggaaatca ttggcaatcc tcccatggca gagccgggat
    12661 accgctggcg cgtgccagca gggcttccag tcgtgcacgt aaaatgcagt ccagtgcgcc
    12721 tttgtcatcg acattcagct gtacgccgcg cacgttgtag cgggcgagaa cgtccagaat
    12781 ggtggtgcga attgcatcgc caaactgttt ctcaacgctg ctattgattt gcaggtcgat
    12841 atcctgcgta tcgagtgggg cgatgcgtat catcacatcc ccagactcaa gggtgcctgc
    12901 aacggcgggc tggtttattt tcatttttca cctgtttctc atgcgggggt cttttgacga
    12961 gctgccgcgt cctggcggga gtgctcaagc aggttctgca aataatgcag cgtgactgca
    13021 gggaccagcg gcgcgatagc cgtgagatcg tttttcgcca gcagttgacg tacccgggaa
    13081 gcggatatcg gcatctcctg gtaacgcagc cgctcaattt caaccagttc gatgggcggt
    13141 gcggagatag tcggcgtttc cagccagtag cgcatatcct ggttgtactg ggcggtaacg
    13201 cgacaaaagg gttcagtacc gacaaagcgg tgagttacac ccagcgcggg agcgaggtac
    13261 tgacggaaaa tcttcagatc aatttcggtg taacaatggt taatgacgct ctgttcttta
    13321 atgaagtagc aagggaacgt agcgcgggag atgatgtatt cggagccgcg atgcacagtc
    13381 aggcgtggaa tatcggcggt gccttttaac accagatcca accggtcttc ataggggaag
    13441 cgtgaagaat cttctttgac taaaaacaga tgcaaccagt cgcactgtgc cgcagcttgt
    13501 tgaatcagat aacggtgacc attcgtgaag ggattggcgt tcatcacaat gcagccaatc
    13561 ttgttccctg gatgacgaaa ttttttcagc gattcggcat agcgtttcag ccgcgtggcg
    13621 ctgttttcca tcagcaccat cacgccgggt acgctggtca gcgtggaaaa accgcactgg
    13681 cggaacagcg cctcgtattc ggttttggta taaataaaca gatgcgtgct gtgccgctca
    13741 taggcgaggt ttatcaattc agtggctaat gtcagcgcca gtccttcacc gcgaacggat
    13801 tcactgatag caacgcattt aataatattt ccggcaat
//
LOCUS       DAJVXP010000113        13558 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVXP010000113 DAJVXP010000000
VERSION     DAJVXP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.113"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="OQS10_003109"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="OQS10_003109"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS2294067.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OQS10_003110"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OQS10_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS2294068.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OQS10_003111"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OQS10_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS2294069.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OQS10_003112"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OQS10_003112"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS2294070.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OQS10_003113"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OQS10_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS2294071.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OQS10_003114"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OQS10_003114"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS2294072.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OQS10_003115"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OQS10_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS2294073.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OQS10_003116"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OQS10_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS2294074.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="OQS10_003117"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="OQS10_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS2294075.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="OQS10_003118"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="OQS10_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS2294076.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="OQS10_003119"
     CDS             complement(11402..11899)
                     /locus_tag="OQS10_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS2294077.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="OQS10_003120"
     CDS             complement(11896..12258)
                     /locus_tag="OQS10_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS2294078.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="OQS10_003121"
     CDS             complement(12248..12595)
                     /locus_tag="OQS10_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS2294079.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="OQS10_003122"
     CDS             12704..13153
                     /locus_tag="OQS10_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294080.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>13558)
                     /locus_tag="OQS10_003123"
     CDS             complement(13402..>13558)
                     /locus_tag="OQS10_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294081.1"
                     /translation="RCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPT
                     EMIQATA"
BASE COUNT         3223 a         3755 c         3521 g         3059 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggg ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggac ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctg
//
LOCUS       DAJVXP010000114        13261 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVXP010000114 DAJVXP010000000
VERSION     DAJVXP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.114"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OQS10_003124"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OQS10_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS2294082.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OQS10_003125"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OQS10_003125"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS2294083.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="OQS10_003126"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="OQS10_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS2294084.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OQS10_003127"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OQS10_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS2294085.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="OQS10_003128"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="OQS10_003128"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS2294086.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="OQS10_003129"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="OQS10_003129"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS2294087.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="OQS10_003130"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="OQS10_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS2294088.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="OQS10_003131"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="OQS10_003131"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS2294089.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="OQS10_003132"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="OQS10_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS2294090.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="OQS10_003133"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="OQS10_003133"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS2294091.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /locus_tag="OQS10_003134"
     CDS             11095..11487
                     /locus_tag="OQS10_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS2294092.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12299
                     /locus_tag="OQS10_003135"
                     /pseudo
     CDS             11491..12299
                     /locus_tag="OQS10_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12335..13189
                     /gene="kdsA"
                     /locus_tag="OQS10_003136"
     CDS             12335..13189
                     /gene="kdsA"
                     /locus_tag="OQS10_003136"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS2294093.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3345 a         3286 c         3394 g         3236 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggctggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacaggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc
    11821 gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat
    11881 gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata
    11941 tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc
    12001 ttgatgaagc tgataacatt gaggtaatcc gcaaattgtt ggatacactc aaagcctcgt
    12061 tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca
    12121 acccggaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg
    12181 aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca
    12241 gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat
    12301 taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg
    12361 cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggtggtatga acgtgctgga
    12421 atctcgcgat ctggcgatgc gcatttgcga gcactacgta accgtgaccc agaaactggg
    12481 tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta
    12541 tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg
    12601 cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt
    12661 ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc
    12721 gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg
    12781 taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg
    12841 tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa
    12901 agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc
    12961 gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat
    13021 ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg
    13081 tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc
    13141 gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct
    13201 tgaaaaataa agccttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa
    13261 a
//
LOCUS       DAJVXP010000115        13260 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVXP010000115 DAJVXP010000000
VERSION     DAJVXP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.115"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OQS10_003137"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OQS10_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS2294094.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OQS10_003138"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OQS10_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS2294095.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OQS10_003139"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OQS10_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS2294096.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OQS10_003140"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OQS10_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS2294097.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OQS10_003141"
     CDS             complement(4036..5568)
                     /locus_tag="OQS10_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS2294098.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OQS10_003142"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OQS10_003142"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS2294099.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OQS10_003143"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OQS10_003143"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS2294100.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OQS10_003144"
     CDS             complement(8663..10399)
                     /locus_tag="OQS10_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS2294101.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OQS10_003145"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OQS10_003145"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS2294102.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OQS10_003146"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OQS10_003146"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS2294103.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OQS10_003147"
     CDS             complement(12121..12855)
                     /locus_tag="OQS10_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS2294104.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="OQS10_003148"
     CDS             13056..>13260
                     /locus_tag="OQS10_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS2294105.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3366 a         3370 c         3488 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAJVXP010000116        12999 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVXP010000116 DAJVXP010000000
VERSION     DAJVXP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.116"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..192
                     /locus_tag="OQS10_003149"
     CDS             <1..192
                     /locus_tag="OQS10_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294106.1"
                     /translation="RFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLR
                     QAGCPTVYPLALASTSVKN"
     gene            99..1394
                     /locus_tag="OQS10_003150"
     CDS             99..1394
                     /locus_tag="OQS10_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS2294107.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1440..2033)
                     /gene="yhgN"
                     /locus_tag="OQS10_003151"
     CDS             complement(1440..2033)
                     /gene="yhgN"
                     /locus_tag="OQS10_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS2294108.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2225..3328
                     /gene="asd"
                     /locus_tag="OQS10_003152"
     CDS             2225..3328
                     /gene="asd"
                     /locus_tag="OQS10_003152"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS2294109.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3601..5787
                     /gene="glgB"
                     /locus_tag="OQS10_003153"
     CDS             3601..5787
                     /gene="glgB"
                     /locus_tag="OQS10_003153"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS2294110.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5784..7757
                     /gene="glgX"
                     /locus_tag="OQS10_003154"
     CDS             5784..7757
                     /gene="glgX"
                     /locus_tag="OQS10_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS2294111.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7775..9070
                     /gene="glgC"
                     /locus_tag="OQS10_003155"
     CDS             7775..9070
                     /gene="glgC"
                     /locus_tag="OQS10_003155"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS2294112.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9070..10503
                     /gene="glgA"
                     /locus_tag="OQS10_003156"
     CDS             9070..10503
                     /gene="glgA"
                     /locus_tag="OQS10_003156"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS2294113.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10522..12969
                     /gene="glgP"
                     /locus_tag="OQS10_003157"
     CDS             10522..12969
                     /gene="glgP"
                     /locus_tag="OQS10_003157"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS2294114.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3229 c         3577 g         3153 t
ORIGIN      
        1 cgtttccacc agtgtcaaaa tctcgacggg gcgtttgtta ttacccctcc tttgatgcct
       61 ggtccggcgt tgctggttga cgatatcgtg gattctgcat ggacgctgac agttctgaca
      121 gcactgttac gccaggcagg ttgcccgacg gtttatcctc ttgcccttgc ttctacctcg
      181 gtaaaaaatt gatgaatctt tcagccaatg cacaagcaac tctcctgcta accagcgatt
      241 tttctcgcgc ggcggcgagt gagtataaac ctcttagtaa tagtgaatgg gggaagtttg
      301 cattatggct gaagcaccaa cgtatcagtc ccgccgagct tctggtgccg caaccgcaag
      361 agaaacttac aggctggagc gatccgcgta tttctcagga gcgtattctt ggcttgctgg
      421 cgcgtggtca tagtctggcg ttggcggtag ataagtggca acgcgccggt ttatggatct
      481 taacccgcgg agatgctgat tatcccgttc gcttgaaaaa ccgattgcga acggatgcac
      541 ctcccgtttt atttggctgc gggaataaag cattactgca agcggaaggt atggcgattg
      601 ttggctcgcg agatgctccg actgacgatt tgcgctatac ccaacaactg gccgcgaaac
      661 tggcccaaca ggggatttgc gttatctctg gtggtgcgcg aggtattgat gaatgtgcaa
      721 tggcgtcggc actggaggcc gggggaactg ccgttggcgt attagctgat agcttgttaa
      781 aaacgagtac gttagtgaaa tggcgtgaag ggcttatagc aggcaacctg gtgttgattt
      841 cgccgtttta cccagaggta cgtttcaccg tcggcaatgc gatggcgcga aataaatata
      901 tttattgcct tgctgaaagc gcaatggttg tacgtgcggg aatgaccggt ggaacgataa
      961 ccggggcgat ggaggcatta aaacatcagt ggctgcctgt gcaggttaaa ccaaatcagg
     1021 atatgcaatc agccaattca cgattagtag aaaatggggc gtcatggagt gctgaacagg
     1081 ctgagaatgt gacgatcaga ctgccagacg ttcctgggct gatgtatgac agagcactcc
     1141 gtaacgctca accagaactg ttttcgctgc atgaagatga cgcaaattac gcagtaatgc
     1201 ccgcgtatac gcctgtcgat ttttatcaac tctttgtggc ggaactggcg atccttgcaa
     1261 aggaatcgat aagtattgaa aggctggcgt cttgtactgg tttaaccatc gaacaaatta
     1321 gtgtgtggct gaaccgcgca gaagaagagg gaagggttat ccgattgggc gaaggtcatt
     1381 atcagttcag gtaataaaac aggtgccgcg tctggttgtg tgcagcacct gtttgaacct
     1441 taccccttca tccacattcg aatgccgtcg aggaacatct gggttgccat catcaccaga
     1501 atcaatccca tcaggcgttc aagtgcgttc acccctttct cgcccagcag acgtaaaaat
     1561 agcgaagact gtagcaggat gacaaaggtg ccgccccagg ccagcagcag agcaatcacc
     1621 agatgtccca tctgattcgg gtactgatga gacaacaaca tcagcgtggc gagaatagtc
     1681 ggcccggcga ctaacggaat tgccaacggc acgataaatg gctcttcacc tgccggaagc
     1741 ccgctgctat ttcctgaagc gctggggaaa atcatcttaa tggcgattag aaacagaatg
     1801 atgccgccag aaatggagac ggtttctgct cgcaggctga gaaatgccag aattttctca
     1861 cccgcaaaca ggaacaccag catcaccagg agagcaataa gcaactctcg caccatgatt
     1921 gcccgccgtc ttttcggttc agtatgtttc agtacggaca tgaaaatagg caggtttccg
     1981 agcggatcca taatcaggat caataaaact gctgcagaaa tgattgcatt cataactcaa
     2041 attccctgat aattgccgcg gactttctgc gtgctaacaa agcaggataa gtcgcattac
     2101 tgatggcttc gctatcattg attaatttca cttgcgactt tggctgcttt ttgtatggtg
     2161 aaagatgtgc caagaggaga ccggcacatt tatacagcac acatctttgc aggaaaaaac
     2221 gcttatgaaa aatgttggtt ttatcggctg gcgcggtatg gtcggctccg ttctcatgca
     2281 acgcatggtt gaagagcgcg acttcgacgc cattcgccct gtcttctttt ctacttctca
     2341 gcttggccag gctgcgccgt cttttggcgg aaccactggc acacttcagg atgcctttga
     2401 tctggaggcg ctaaaggccc tcgatatcat tgtgacctgt cagggcggcg attataccaa
     2461 cgaaatctat ccaaagcttc gtgaaagcgg atggcaaggt tactggattg acgcagcatc
     2521 gtctctgcgc atgaaagatg acgccatcat cattcttgac cccgtcaatc aggacgtcat
     2581 taccgacgga ttaaataatg gcatcaggac ttttgttggc ggtaactgta ccgtaagcct
     2641 gatgttgatg tcgttgggtg gtttattcgc caatgatctt gttgattggg tgtccgttgc
     2701 aacctaccag gccgcttccg gcggtggtgc gcgacatatg cgtgagttat taacccagat
     2761 gggccatctg tatggccatg tggcagatga actcgcgacc ccgtcctctg ctattctcga
     2821 tatcgaacgc aaagtcacaa ccttaacccg tagcggtgag ctgccggtgg ataactttgg
     2881 cgtgccgctg gcgggtagcc tgattccgtg gatcgacaaa cagctcgata acggtcagag
     2941 ccgcgaagag tggaaagggc aggcggaaac caacaagatc ctcaacacat cttccgtaat
     3001 tccggtagat ggtttatgtg tgcgtgtcgg ggcattgcgc tgccacagcc aggcattcac
     3061 tattaaattg aaaaaagatg tgtctattcc gaccgtcgaa gaactgctgg ctgcgcacaa
     3121 tccgtgggcg aaagtcgttc cgaacgatcg ggaaatcact atgcgtgagc taaccccagc
     3181 tgccgttacc ggcacgctga ccacgccggt aggccgcctg cgtaagctga atatgggacc
     3241 agagttcctg tcagccttta ccgtgggcga ccagctgctg tggggggccg cggagccgct
     3301 gcgtcggatg cttcgtcaac tggcgtaatc tttattcatt aaatctgggg cgcgatgccg
     3361 cccctgttag tgcgtaatac aggagtaagc gcagatgttt catgatttac cgggagttaa
     3421 atagagcatt ggctattctt taagggtggc tgaatacatg agtattcaca gccttacctg
     3481 aagtgaggac gacgcagaga ggatgcacag agtgctgcgc cgttcaggtc aaaaaaatgt
     3541 cacaaccaga agtcaaaaat ccaattggat ggggtgacac aataaaacag gaagacaagc
     3601 atgtccgatc gtatcgatag agacgtgatt aacgcgctaa ttgcaggcca ttttgcggat
     3661 cctttttccg tactgggaat gcataaaacc accgcgggac tggaagtccg tgccctttta
     3721 cccgacgcta ccgatgtgtg ggtgattgaa ccgaaaaccg ggcgcaaact cgcaaaactg
     3781 gagtgtctcg actcacgggg attctttagc ggcgtcattc cgcgacgtaa gaattttttc
     3841 cgctatcagt tggctgttgt ctggcatggt cagcaaaacc tgattgatga tccttaccgt
     3901 tttggtccgc taatccagga aatggatgcc tggctattat ctgaaggtac tcacctgcgc
     3961 ccgtatgaaa ccttaggcgc gcatgcagat actatggatg gcgtcacagg tacgcgtttt
     4021 tctgtctggg ctccaaacgc ccgtcgggtc tcggtggttg ggcaattcaa ctactgggac
     4081 ggtcgccgtc acccgatgcg cctgcgtaaa gagagcggca tttgggaact gtttatccct
     4141 ggggcgcata acggtcagct ctataaatac gagatgattg atgccaatgg caacttgcgt
     4201 ctgaagtccg acccttatgc cttcgaagcg caaatgcgcc cggaaaccgc gtctcttatt
     4261 tgcgggctgc cggaaaaggt tgtacagact gaagagcgca aaaaagcgaa tcagtttgat
     4321 gcgccaatct ctatttatga agttcacctg ggctcctggc gtcgccacac cgataacaat
     4381 ttctggttga gctaccgcga gctggccgat caactggtgc cttatgctaa atggatgggc
     4441 tttacccacc tcgaactact gcccattaac gagcatccct tcgatggcag ttggggttat
     4501 cagccaaccg gcctgtatgc accaacccgc cgttttggta ctcgcgacga cttccgttat
     4561 ttcattgatg ccgcacacgc agcgggtctg aacgtgattc tcgactgggt gccaggccac
     4621 ttcccgaccg atgactttgc gcttgccgaa tttgatggca cgaacttgta tgaacacagc
     4681 gatccgcgcg aaggctatca tcaggactgg aatacgctga tctacaacta tggtcgccgt
     4741 gaagtcagta acttcctcgt cggtaacgcg ctttactgga ttgaacgttt tggtattgat
     4801 gcgctgcgcg tcgatgcggt ggcgtcaatg atttatcgcg actacagccg taaagagggg
     4861 gagtggatcc cgaacgaatt tggcgggcgc gagaatcttg aagcgattga attcttgcgt
     4921 aataccaacc gtattcttgg tgagcaggtt tccggtgcgg tgacaatggc tgaggagtct
     4981 accgatttcc ctggcgtttc tcgtccgcag gatatgggcg gtctgggctt ctggtacaag
     5041 tggaacctcg gctggatgca tgacaccctg gactacatga agctcgaccc ggtttatcgt
     5101 cagtatcatc acgataaact caccttcggg attctctaca actacactga aaacttcgtc
     5161 ctgccgttgt cgcatgatga agtggtccac ggtaaaaaat cgattctcga ccgcatgccg
     5221 ggcgacgcat ggcagaaatt cgctaacctg cgcgcctact atggctggat gtgggcattc
     5281 ccgggcaaga aactgctgtt catggggaac gaatttgccc aaggccgtga gtggaaccat
     5341 gacgccagcc tcgactggca tctgttggaa ggcggcgata actggcacca cggtgtccag
     5401 cgtctggtgc gcgatctgaa cctcacctac cgccaccata aagcaatgca tgaactggat
     5461 tttgacccgt acggctttga atggctagtg gtggatgaca aagaacgctc ggtgctgatc
     5521 tttgtgcgtc gcgataaaga gggtaacgaa attatcgttg ccagtaactt tacgccggtg
     5581 ccgcgtcatg attatcgctt cggcattaat cagccaggca aatggcgtga aatcctcaat
     5641 accgattcca tgcactatca cggcagtaat gcaggcaatg gcggcacggt acacagcgat
     5701 gagattgcca gccacggtcg tcagcattca ctaagcctga cactaccacc gctggccact
     5761 atctggctgg ttcgggaggc agaatgacac gactcgccat tggcaaaccc gctcccctcg
     5821 gcgcgcatta cgacggtcag ggcgtcaact tcacactttt ctccgctcat gccgagcggg
     5881 tagagctgtg tgtctttgac gccaatggcc aggaacatcg ctatgacttg ccagggcaca
     5941 gtggcgacat ttggcacggt tatctgccgg atgcgcgccc gggtttgcgt tatggttatc
     6001 gcgttcatgg cccctggcaa cccgccgagg ggcatcgctt taacccggcg aagttgttga
     6061 ttgatccttg cgcgcggcaa attgaagggg aattcaaaga taacccgctg ctgcacgccg
     6121 gtcataatga acctgactat cgcgacaacg ccgccattgc gccgaaatgc gtagtggtgg
     6181 ttgatcacta tgactgggaa gatgatgccc cgccgcgcac gccgtggggc agcaccatca
     6241 tttatgaagc ccatgttaaa ggattaacgt acctgcaccc ggagatcccg gtcgagatcc
     6301 gtggcactta taaagccctc gggcatccgg tgatgatcaa ctatttgaaa cagttgggca
     6361 ttaccgcgct ggaactgctg ccggtggcgc agtttgccag tgaaccacgt ctgcaacgca
     6421 tggggctaag taactactgg ggttacaacc cggtggcgat gtttgcgctg catccggcgt
     6481 atgcctgctc gccagaaacg gcgctgcatg agtttcgcga tgcaatcaaa gcactgcata
     6541 aagcgggtat cgaagtcatt cttgatatcg tgctcaacca tagtgcggaa ctggacctcg
     6601 acggcccgtt attctcgctg cgtgggatcg acaaccgtag ctattattgg ataagagaag
     6661 acggcgatta tcacaactgg accggttgtg gcaacacgct caatttgagt catccggcgg
     6721 tggtggatta tgcctgcgcc tgcctgcgtt attgggtaga aacctgtcac gtcgatggtt
     6781 tccgctttga tctggcggca gtcatgggcc gtacgccaga gttccgtcag gatgcgccgt
     6841 tgtttaccgc tatccagaac tgcccggtgc tctcgcaggt gaagttaatt gctgaaccgt
     6901 gggatatcgc ccttggtggt tatcaggtgg gaaatttccc gccgctgttt gccgagtgga
     6961 acgatcattt ccgcgatgct gcccgtcgtt tctggctaca ttatgatttg cctctggggg
     7021 cgtttgccgg gcgttttgct gcctccagcg atgtttttaa acgtaatggt cgtctgccga
     7081 gtgccgcgat taatctcgtc actgcgcatg acggttttac gcttcgcgac tgcgtttgct
     7141 tcaaccataa acacaatgaa gcaaacggag aagaaaatcg cgacgggacc aacaacaatt
     7201 acagtaacaa tcatggtaaa gaagggttag gcggttctct tgacctggtt gaacggcggc
     7261 gcgacagtat tcacgccctg ttaacaacgt tgttgctctc ccagggtacg ccgatgttac
     7321 tggccggtga cgaacacggt cacagccagc atggcaataa caatgcctac tgtcaggata
     7381 accaattaac ctggttggac tggtcgcagg caagcagtgg tttaaccgca tttaccgccg
     7441 cgttaatcca tctgcgcaag cgcattcccg ctttggtgga gaatcgctgg tgggaagaag
     7501 gcgacggcaa tgtccgttgg ctaaatcgat atgctcaacc tttaagcacg gatgagtggc
     7561 aaaacgggcc gaaacagctg caaattctgc tctcggatcg ctttttgatc gcaattaacg
     7621 ccacgcttga ggtaacagag attgttttac ctgctgggga gtggcacgcc attcccccat
     7681 tcgctggaga ggataaccca gtgattacgg ctgtctggca gggacctgca cacggattgt
     7741 gtgtgttcca gagatgataa aaaaggagtt agtcatggtt agtttagaga agaacgatca
     7801 cttaatgttg gcgcgccagc tgccattgaa atctgttgcc ctgatactgg cgggaggacg
     7861 tggtacccgc ctgaaggatt taaccaataa gcgagcaaaa ccggccgtac acttcggcgg
     7921 taagttccgc attattgact ttgcgctgtc taactgcatc aactctggga tccgtcgtat
     7981 gggcgtgatc acccagtacc agtcccacac tctggtgcag cacattcagc gcggctggtc
     8041 attcttcaat gaagaaatga acgagtttgt cgatctgctg ccagcacagc agagaatgaa
     8101 aggggaaaac tggtatcgcg gcaccgcaga tgcggtcacc caaaacctcg acattatccg
     8161 ccgttataaa gcggaatacg tggtgatcct ggcgggcgac catatctaca agcaagacta
     8221 ctcgcgtatg cttatcgatc acgtcgaaaa aggcgcacgt tgcaccgttg cttgtatgcc
     8281 agtaccgatt gaagaagcct ccgcatttgg cgttatggcg gttgatgaga acgataaaat
     8341 tatcgaattc gttgaaaaac ctgctaaccc accgtcaatg ccgaacgatc cgagcaaatc
     8401 tctggcgagt atgggtatct acgtctttga cgccgactat ctgtatgaac tgctggaaga
     8461 agacgatcgt gatgagaact ccagccacga ctttggcaaa gatttgattc ccaagatcac
     8521 cgaagccggt ctggcctatg cgcacccgtt cccgctctct tgcgtacaat ccgacccgga
     8581 tgccgagccg tactggcgcg atgtgggtac gctggaagct tactggaaag cgaacctcga
     8641 tctggcctct gtggtgccgg aactggatat gtacgatcgc aattggccaa ttcgcaccta
     8701 caatgaatca ttaccgccag cgaaattcgt gcaggatcgc tccggtagcc acgggatgac
     8761 ccttaactca ctggtatccg gcggctgtgt gatctccggt tcggtggtgg tgcagtccgt
     8821 tctgttctcg cgcgttcgcg tgaattcatt ctgcaacatt gattccgccg tattgttacc
     8881 ggaagtatgg gtaggtcgct cgtgccgtct gcgccgctgc gtcatcgatc gtgcttgtgt
     8941 tattccggaa ggcatggtga ttggtgaaaa cgcagaggaa gatgcacgtc gtttctatcg
     9001 ttcagaagaa ggcatcgtgc tggtaacgcg cgaaatgcta cggaagttag ggcataaaca
     9061 ggagcgataa tgcaggtttt acatgtatgt tcagagatgt tcccgctgct taaaaccggc
     9121 ggtctggctg atgttattgg ggcattaccc gcagcacaaa tcgcagacgg cgttgacgct
     9181 cgcgtactgt tgcctgcatt tcccgatatt cgccgtggcg tgaccgatgc gcaggtagta
     9241 tcccgtcgtg ataccttcgc cggacatatc acgctgttgt tcggtcatta caacggggtt
     9301 ggcatatacc tgattgacgc gccgcatctc tatgatcgtc cgggaagccc gtatcacgat
     9361 accaacttat ttgcctatac cgacaacgta ttgcgttttg cgcttctggg gtgggttggg
     9421 gcagaaatgg ccagcgggct tgacccattc tggcgtcctg atgtggtgca tgcgcacgac
     9481 tggcatgcag gccttgcgcc tgcgtatctg gcggcgcgcg ggcgtcctgc gaagtcggtg
     9541 tttactgtgc acaacctggc ctatcaaggc atgttttatg cacatcacat gaatgacatc
     9601 caattgccat ggtcattctt taatattcat gggctggaat tcaacggaca aatctctttc
     9661 ctgaaggccg gtctgtacta tgccgatcac attacggcgg tcagtccaac ctacgctcgc
     9721 gagatcaccg aaccgcagtt tgcctacggt atggaaggtc tgttgcaaca gcgtcatcgt
     9781 gaagggcgtc tttccggcgt actgaacggc gtggacgaga aaatctggag tccagagacg
     9841 gacttactgt tggcctcgcg ctacacccgc gatacgttgg aagataaagc ggaaaataag
     9901 cgccagttac aaatcgcaat ggggcttaag gttgacgata aagtgccgct ttttgcagtg
     9961 gtgagccgtc tgaccagcca gaaaggtctc gacctggtgc tggaagcctt accgggtctt
    10021 ctggagcagg gcgggcagct ggcgctactc ggcgcgggcg atccggtgct gcaggaaggt
    10081 ttccttgcgg cggcagcgga ataccccggt caggtgggcg ttcagattgg ctatcacgaa
    10141 gcattttcgc atcgcattat gggcggcgcg gacgtcattc tggtgcccag ccgttttgaa
    10201 ccgtgcggct taacgcaact ttatggattg aagtacggta cgctgccgtt agtgcggcgc
    10261 accggtgggc ttgctgatac ggtttctgac tgttctcttg agaaccttgc agatggcgtc
    10321 gccagtgggt ttgtctttga agataataat gcctggtcgc tgttacgggc tattcggcgt
    10381 gcttttgtac tgtggtcccg tccttcactg tggcggtttg tgcaacgtca ggctatggca
    10441 atggatttta gctggcaggt cgcggcgaag tcgtaccgtg agctttacta tcgcttgaaa
    10501 tagttttcag gaaacgccta tatgaatgct ccgtttacat attcatcgcc cacgcttagc
    10561 gtagaagctc ttaagcactc tatcgcttac aagctgatgt ttacgattgg aaaggacccg
    10621 gtcgtcgcca ataaacatga atggctgaac gcaacgttat ttgctgtgcg cgatcgtctc
    10681 gtggagcgct ggttacgttc aaaccgtgcc cagttgtcgc aagaaacacg tcaggtttac
    10741 tacctgtcga tggagttttt gattggccgt acgctctcca acgccatgtt gtcgctagga
    10801 atttacgaag atgtacaggg cgcactggaa gcgatggggt taaatctcga agagctgatt
    10861 gatgaagaaa atgacccagg cctcggtaac ggtggcctgg gacgtctggc ggcttgcttc
    10921 cttgattctc tggcgacgtt agggttgccg gggcgcggtt acggcatccg ctatgactac
    10981 ggtatgttca agcagaacat cgttaacggt agccagaaag agtcgccaga ctactggctg
    11041 gaatacggta acccgtggga attcaaacgc cacaacacgc gctataaagt ccgttttggc
    11101 ggtcgcattc agcaggaagg taaaaaaacg cgctggattg aaaccgaaga gattctggga
    11161 gtcgcttacg atcagataat ccctggttac gacaccgacg cgaccaacac gctgcgtttg
    11221 tggagtgcgc aagccagtag cgaaattaac ctcggtaaat tcaaccaggg tgactacttc
    11281 gcggcagtgg aagataaaaa ccactccgag aacgtatctc gcgtactgta tccggatgac
    11341 tccacctact ccgggcgtga gctgcgcctg cgtcaggaat acttcctggt ttcctcgacc
    11401 attcaggaca ttttaagccg ccattatcag ttgcataaaa cctacgataa cctggcggat
    11461 aaaatcgcga ttcatctcaa tgatacccat ccggtactgt cgattcctga gatgatgcgt
    11521 ctgctgatcg atgagcacca atttagctgg gacgacgcgt ttgaggtgtg ttgtcaggtc
    11581 ttctcctaca ctaaccacac gctgatgagc gaggcgctgg aaacctggcc ggttgatatg
    11641 ctgggtaaaa ttctgccgcg tcacctgcag atcatctttg aaatcaacga ctatttcctg
    11701 aaaaccttgc aggaacagta tccgaacgat accgatctgc tgggacgggc gtcgatcatt
    11761 gatgaatcca acggtcgtcg tgtgcgtatg gcctggctgg cggttgttgt gagccacaaa
    11821 gttaacggtg tatcggaact gcactctaat ctgatggtgc aatcgttgtt tgccgacttt
    11881 gcgaaaatct tcccgggtcg tttcaccaac gtcaccaacg gtgtgacgcc gcgtcgctgg
    11941 ctggcggtag cgaacccatc gctttcagcc gtgctggacg aacacctggg ccgtaactgg
    12001 cgcaccgacc ttagcctgct taatgagctg caacaacact gtgatttccc aatggttaat
    12061 cacgctgtgc atcaggcgaa gctggagaac aaaaagcgtc tggcagagta tatcgcccag
    12121 cagctgaatg tggtggtgaa tccaaaggcg ttgttcgatg tacaaatcaa acgtattcac
    12181 gaatacaaac gtcaattgat gaatgtgttg catgtgatta cccgctataa ccgcatcaag
    12241 gccgacccgg atgcgaagtg ggtaccgcgc gtgaatattt ttggcggtaa ggcggcttcg
    12301 gcctattaca tggcgaagca cattattcat ttgatcaatg acgtagcgaa agtgatcaac
    12361 aacgatccgc agattggcga taagctgaaa gtcgtgttca tcccgaacta cagcgttagc
    12421 ctggcgcagt tgatcattcc ggcggcagat ctgtctgaac agatttcgct ggcagggacg
    12481 gaagcttccg gcaccagtaa catgaagttt gcgcttaacg gtgcgctgac tatcggtacg
    12541 ttggacggtg cgaatgtcga gatgcgggat catgtcggtg ctgacaatat ctttattttt
    12601 ggtaacacag cggaagaagt ggaagaactg cgtcgtcagg gctacaaacc gcgtgaatac
    12661 tacgagaaag atgaggagct gcatcaggtg ctgacgcaaa tcggcagcgg tgtattcagt
    12721 ccggaagatc cgggtcgcta tcgcgatctg gttgattcgc tgatcaactt cggcgatcac
    12781 tatcaggttc tggcggatta tcgcagctat gtcgattgtc aggataaagt cgacgaactc
    12841 tacgagcttc aggaagagtg gaccgcaaaa gcgatgctga acattgccaa tatgggctac
    12901 ttctcttctg accgtactat caaagagtac gccgatcata tctggcatat cgatccggtg
    12961 agattgtaag ttcaccaata aatagaacgg ggccaaagg
//
LOCUS       DAJVXP010000117        12449 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVXP010000117 DAJVXP010000000
VERSION     DAJVXP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.117"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="OQS10_003158"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="OQS10_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS2294115.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="OQS10_003159"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="OQS10_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS2294116.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="OQS10_003160"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="OQS10_003160"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS2294117.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="OQS10_003161"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="OQS10_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="OQS10_003162"
     CDS             3566..3850
                     /locus_tag="OQS10_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS2294118.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OQS10_003163"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OQS10_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS2294119.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OQS10_003164"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OQS10_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS2294120.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OQS10_003165"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OQS10_003165"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS2294121.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="OQS10_003166"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="OQS10_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS2294122.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10040..11140
                     /gene="ompC"
                     /locus_tag="OQS10_003167"
     CDS             10040..11140
                     /gene="ompC"
                     /locus_tag="OQS10_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS2294123.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11304..11786
                     /locus_tag="OQS10_003168"
                     /pseudo
     CDS             11304..11786
                     /locus_tag="OQS10_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11901..>12449
                     /locus_tag="OQS10_003169"
     CDS             11901..>12449
                     /locus_tag="OQS10_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS2294124.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHN"
BASE COUNT         3231 a         3104 c         2967 g         3147 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatatcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca atattaaaaa
     9841 ataaatttca aataccagta attcacctta tatctcattt cgacttcgat cacatatcga
     9901 taaataatat tatttcatct atttttctca ttgtttttaa tcaccattct attttaaaaa
     9961 cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata
    10021 attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt
    10081 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct
    10141 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
    10201 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
    10261 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa
    10321 caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg
    10381 tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg
    10441 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac
    10501 ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca
    10561 aggcaaaaat gaacgtactg acaacagtca tctttatggt gctgactaca cgcgtgccaa
    10621 cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
    10681 atataccaaa tccgatcgga caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc
    10741 ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta
    10801 ctttgcagct aattacgctg aaacattaaa catgaccacc tatggggatg gttatatctc
    10861 taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc
    10921 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacggcg accaggacat
    10981 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
    11041 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcggac
    11101 cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc
    11161 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
    11221 atttttgata tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat
    11281 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
    11341 ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt
    11401 tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa
    11461 ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
    11521 atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa
    11581 acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca
    11641 gcgttaataa aattaacacg ataacctttc caaaagaatc aaaactggaa aacatttata
    11701 ttgatcataa caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg
    11761 aagcgcaaaa taacaaactg acaaaaatta atatttctga tagttataaa ctgaaatttc
    11821 ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa tctctgattg
    11881 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
    11941 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
    12001 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
    12061 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
    12121 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
    12181 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
    12241 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
    12301 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
    12361 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
    12421 cagttactta ttttagtgcg gcacataat
//
LOCUS       DAJVXP010000118        12409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVXP010000118 DAJVXP010000000
VERSION     DAJVXP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.118"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQS10_003170"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQS10_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS2294125.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQS10_003171"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQS10_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS2294126.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OQS10_003172"
     CDS             complement(2659..5772)
                     /locus_tag="OQS10_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS2294127.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OQS10_003173"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OQS10_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS2294128.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OQS10_003174"
     CDS             7014..7292
                     /locus_tag="OQS10_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294129.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OQS10_003175"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OQS10_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS2294130.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OQS10_003176"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OQS10_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS2294131.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OQS10_003177"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OQS10_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS2294132.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OQS10_003178"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OQS10_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS2294133.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OQS10_003179"
     CDS             10284..10931
                     /locus_tag="OQS10_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCS2294134.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="OQS10_003180"
     CDS             complement(10973..11503)
                     /locus_tag="OQS10_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2294135.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OQS10_003181"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OQS10_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS2294136.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2753 c         2808 g         3418 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAJVXP010000119        12037 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVXP010000119 DAJVXP010000000
VERSION     DAJVXP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.119"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            9..323
                     /locus_tag="OQS10_003182"
     CDS             9..323
                     /locus_tag="OQS10_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2294137.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="OQS10_003183"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="OQS10_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="OQS10_003184"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="OQS10_003184"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS2294138.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="OQS10_003185"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="OQS10_003185"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS2294139.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OQS10_003186"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OQS10_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS2294140.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OQS10_003187"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OQS10_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS2294141.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OQS10_003188"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OQS10_003188"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS2294142.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="OQS10_003189"
     CDS             8424..8927
                     /locus_tag="OQS10_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS2294143.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="OQS10_003190"
     CDS             9004..9687
                     /locus_tag="OQS10_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS2294144.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="OQS10_003191"
     CDS             9766..10752
                     /locus_tag="OQS10_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS2294145.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="OQS10_003192"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="OQS10_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS2294146.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAJVXP010000120        11960 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVXP010000120 DAJVXP010000000
VERSION     DAJVXP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.120"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1760
                     /locus_tag="OQS10_003193"
     CDS             <1..1760
                     /locus_tag="OQS10_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCS2294147.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="OQS10_003194"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="OQS10_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS2294148.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="OQS10_003195"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="OQS10_003195"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS2294149.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="OQS10_003196"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="OQS10_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS2294150.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="OQS10_003197"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="OQS10_003197"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS2294151.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="OQS10_003198"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="OQS10_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS2294152.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="OQS10_003199"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="OQS10_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS2294153.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="OQS10_003200"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="OQS10_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS2294154.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="OQS10_003201"
     CDS             complement(10054..10212)
                     /locus_tag="OQS10_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS2294155.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="OQS10_003202"
     CDS             10395..10694
                     /locus_tag="OQS10_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2294156.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="OQS10_003203"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="OQS10_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS2294157.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="OQS10_003204"
     CDS             complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="OQS10_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS2294158.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2985 a         2947 c         3078 g         2950 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat
    11281 caggaaatcg tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg
    11341 gccctgaccg gtccaggttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg
    11401 acgcggctgg cgttttttac cagcgcgtgg tgcagcagca gagctattac ccaataactc
    11461 ttccgggtta attccgtcag ccttcatcag ctccagccag gtgctaattt tttcctgacg
    11521 ctcagccagt tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct
    11581 gaatttttcg agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag
    11641 ggtgcgaatg ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg
    11701 ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa
    11761 atttaatcta tttacagtct tgcgcgagat tatttcagct gttattttct gaaactctca
    11821 gaataatttc aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca
    11881 gaacggcgct ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg
    11941 caatataaca gaataaaaga
//
LOCUS       DAJVXP010000121        11799 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVXP010000121 DAJVXP010000000
VERSION     DAJVXP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.121"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..477
                     /locus_tag="OQS10_003205"
     CDS             <1..477
                     /locus_tag="OQS10_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2294159.1"
                     /translation="RNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKS
                     EITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRNPY
                     HTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            529..1464
                     /gene="ttcA"
                     /locus_tag="OQS10_003206"
     CDS             529..1464
                     /gene="ttcA"
                     /locus_tag="OQS10_003206"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS2294160.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1593..2966)
                     /gene="dbpA"
                     /locus_tag="OQS10_003207"
     CDS             complement(1593..2966)
                     /gene="dbpA"
                     /locus_tag="OQS10_003207"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS2294161.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3444..4427)
                     /gene="zntB"
                     /locus_tag="OQS10_003208"
     CDS             complement(3444..4427)
                     /gene="zntB"
                     /locus_tag="OQS10_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS2294162.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4682..5914
                     /gene="dgcM"
                     /locus_tag="OQS10_003209"
     CDS             4682..5914
                     /gene="dgcM"
                     /locus_tag="OQS10_003209"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS2294163.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5935..6498)
                     /gene="smrA"
                     /locus_tag="OQS10_003210"
     CDS             complement(5935..6498)
                     /gene="smrA"
                     /locus_tag="OQS10_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS2294164.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6828..7736)
                     /locus_tag="OQS10_003211"
     CDS             complement(6828..7736)
                     /locus_tag="OQS10_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2294165.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7912..9222
                     /gene="abgA"
                     /locus_tag="OQS10_003212"
     CDS             7912..9222
                     /gene="abgA"
                     /locus_tag="OQS10_003212"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCS2294166.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9222..10667
                     /gene="abgB"
                     /locus_tag="OQS10_003213"
     CDS             9222..10667
                     /gene="abgB"
                     /locus_tag="OQS10_003213"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS2294167.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10704..>11799
                     /locus_tag="OQS10_003214"
     CDS             10704..>11799
                     /locus_tag="OQS10_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS2294168.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
BASE COUNT         3001 a         2998 c         2926 g         2874 t
ORIGIN      
        1 agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg gatccggacg
       61 gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact gaccgggcat
      121 catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga aaagcaaaaa
      181 ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta ttactcggta
      241 agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg ccgccgtaat
      301 ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc gaacccggca
      361 tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat ttacggtatg
      421 tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt atcgtagttg
      481 caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat gcaagaaaat
      541 caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt
      601 aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt
      661 tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa
      721 agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc
      781 ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgtcgaa
      841 gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg
      901 tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg ggcgacgaag
      961 atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa tatgttctac
     1021 ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt
     1081 attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg
     1141 ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt
     1201 gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc
     1261 gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga tttcaaaggc
     1321 atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc
     1381 ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacgg
     1441 ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg tatcgggcta
     1501 ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc gaataagatg
     1561 tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac ccggctcgtt
     1621 tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac agcctgacgg
     1681 accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc gccatcaagc
     1741 ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc ttttttccca
     1801 ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact attagctggc
     1861 ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc ccgctgtgct
     1921 tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc tgtacggccg
     1981 atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac cagctcaagc
     2041 gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg ggcgctaccg
     2101 ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc gtgtaatgac
     2161 aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc ttttttggta
     2221 ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg caacagagga
     2281 attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa agcatctgtt
     2341 gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac ggcaatggct
     2401 tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa acggatgaca
     2461 tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc catcaccaaa
     2521 gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa acgacccggc
     2581 gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc gaacggttga
     2641 ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg acgcaattca
     2701 cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg ggtttgaaat
     2761 agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt gccgctgccg
     2821 gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc ggcggcctgc
     2881 accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc gggaggcaaa
     2941 acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg caaagttagc
     3001 aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt tcttcatcgg
     3061 gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga acaggatcgc
     3121 caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata tttccctccg
     3181 gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa aaaagccgac
     3241 tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa aaaggaagta
     3301 agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa gggagatatt
     3361 gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt tttgtataca
     3421 gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca taaagcaaca
     3481 ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccacccgccg
     3541 ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact gggtaaaaag
     3601 accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg catcacctga
     3661 gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc gtcaaggccg
     3721 cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc
     3781 aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata gcgacgcatc
     3841 acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcaagg
     3901 agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc gctggaatga
     3961 tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg acccgtgccc
     4021 tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca
     4081 attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg ttcatcggtg
     4141 ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct gactcggggc
     4201 cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc
     4261 agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca
     4321 tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca
     4381 aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt gcctccatca
     4441 tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca
     4501 ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt
     4561 ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca
     4621 tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata acgggatctc
     4681 aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct ggatcgtttc
     4741 gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga tgcaagacct
     4801 cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag aactgccgaa
     4861 atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg ttcagcgtaa
     4921 agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag aagcagaacc
     4981 ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag ccagtcgctc
     5041 ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc
     5101 accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta acctcgccgc
     5161 gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct gggaaataaa
     5221 tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac ccggtggtca
     5281 taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg tgcagaccta
     5341 cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac atgatattac
     5401 tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg cgatgaccgg
     5461 attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc agcatctcgc
     5521 catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga
     5581 tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca ccctcgaaag
     5641 ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct
     5701 accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg taagcgtggc
     5761 aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg cgcatcacga
     5821 aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt atcgggcgaa
     5881 aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc gagatcaacg
     5941 actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt tacgtagtgc
     6001 gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg cctgaacatc
     6061 atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat gcgatttatc
     6121 atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca tggcttgttg
     6181 aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct
     6241 cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt gcaacccttc
     6301 ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg tggtgaggaa
     6361 attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg ttggatgcca
     6421 gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa gaaacagcga
     6481 tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat gataaccaga
     6541 ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa atattgccga
     6601 tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa tgtaacttat
     6661 attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac cgttataaca
     6721 cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact gctgtgtgta
     6781 ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta actttgcaga
     6841 tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt ctggcgacta
     6901 tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac cggcaacatc
     6961 accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat gctgagaaaa
     7021 tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac accgacctgt
     7081 ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact gccgtgtggc
     7141 gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc aatggcgggg
     7201 tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa agtaaattcg
     7261 tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc ctgacgcaat
     7321 tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg cggatgctgc
     7381 tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc ccccatgccg
     7441 atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg ggctgcgcgc
     7501 agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc agttaacgtc
     7561 acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag ctcctgaata
     7621 gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg aatgctgccc
     7681 tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa agccatgacg
     7741 ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa tgcagatgat
     7801 tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg ataagtaaaa
     7861 actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt tatggagtct
     7921 ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg tgattttcat
     7981 cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc ggaagaattg
     8041 cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag tagccggatg
     8101 ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca acagggtgcg
     8161 ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc caccctggat
     8221 accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct ggatctcagt
     8281 gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg taacgccgga
     8341 atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct ggcgcatacc
     8401 cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca gcctgcagag
     8461 gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga tgttgattat
     8521 tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg cggcagtgat
     8581 aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc tcacgcaggc
     8641 gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac tcttgcactg
     8701 catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt tatgcaggca
     8761 ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac acgcggggcc
     8821 agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca gggcgcagca
     8881 accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc cagttctcct
     8941 tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg ggtcaatcag
     9001 gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat gatggcccgc
     9061 gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct ggcggcaggt
     9121 catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt cgaaacgctg
     9181 gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa atctatcgtt
     9241 ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc gatcaaatct
     9301 gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg gcttcggctc
     9361 tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat gcctttattg
     9421 cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac gccctggcag
     9481 gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt gaaaatggtc
     9541 acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata gccgtcaaga
     9601 aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt cctggcgaag
     9661 aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat gtggatgcgg
     9721 cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg ctggcaaaca
     9781 ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc cctcatttgg
     9841 gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc ctcaacgaac
     9901 atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg atctcgccca
     9961 acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa atgaccgacg
    10021 tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg atgaccgaaa
    10081 ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat cgcaccttag
    10141 aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac tccgaagaac
    10201 tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa aacagtctga
    10261 ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt catcgtgaaa
    10321 cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt gcggcatcga
    10381 ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc ccctgttttg
    10441 ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca tctattgctc
    10501 ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat ctcttcattg
    10561 attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac acgcaaccgt
    10621 atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa caacaacgca
    10681 aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc tcccaatccg
    10741 ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct tttttgctct
    10801 ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc tttggcgtca
    10861 gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc agtgtggaag
    10921 gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct ccacttggtg
    10981 cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta ctgccagcgc
    11041 taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg gtgctgttta
    11101 ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta ccgatgggtg
    11161 cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct gccattgcag
    11221 gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg ttgctgtcgg
    11281 ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt gtaattgata
    11341 actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc ctgataaccg
    11401 acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag aaactgcaga
    11461 cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg ctacttttta
    11521 ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat ccgattaatc
    11581 acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt ctttttttct
    11641 ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag gcggatttac
    11701 cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg gtttttcccc
    11761 tcgcccagtt tgtcgccatg tttaactgga gcaacatgg
//
LOCUS       DAJVXP010000122        11540 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVXP010000122 DAJVXP010000000
VERSION     DAJVXP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.122"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            459..1385
                     /locus_tag="OQS10_003215"
     CDS             459..1385
                     /locus_tag="OQS10_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294169.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1335..2528)
                     /locus_tag="OQS10_003216"
     CDS             complement(1335..2528)
                     /locus_tag="OQS10_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2294170.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2664..4388
                     /gene="iucA"
                     /locus_tag="OQS10_003217"
     CDS             2664..4388
                     /gene="iucA"
                     /locus_tag="OQS10_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS2294171.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4389..5336
                     /gene="iucB"
                     /locus_tag="OQS10_003218"
     CDS             4389..5336
                     /gene="iucB"
                     /locus_tag="OQS10_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS2294172.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5336..7078
                     /gene="iucC"
                     /locus_tag="OQS10_003219"
     CDS             5336..7078
                     /gene="iucC"
                     /locus_tag="OQS10_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS2294173.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7075..8412
                     /gene="iucD"
                     /locus_tag="OQS10_003220"
     CDS             7075..8412
                     /gene="iucD"
                     /locus_tag="OQS10_003220"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS2294174.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8418..10613
                     /gene="iutA"
                     /locus_tag="OQS10_003221"
     CDS             8418..10613
                     /gene="iutA"
                     /locus_tag="OQS10_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS2294175.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11164..11307)
                     /locus_tag="OQS10_003222"
     CDS             complement(11164..11307)
                     /locus_tag="OQS10_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294176.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2927 a         2894 c         2949 g         2770 t
ORIGIN      
        1 tcaacgaagg ggccgacaaa cacgttgtcc ctgagttcac attcataaat gttgaccggt
       61 ttgacaatac ggacattgat cccataaatc acatcccgga cagcacattc cagaaatttc
      121 atactcacac gtcctttaaa tacagatgac atttgccgga attataccgc ccagattcac
      181 ttgccatgtt tcagaatagc ttataagccg cacttttacc gatactcagc aacaaatcag
      241 gtctgtatcc acttcagccg gaaaccggat ctgcgcggcg gggaacgggc aagaaatatg
      301 ataattaagc aacacacata aaatcatatt gcataatcgc atctgaatcg aacacttatt
      361 cgcatcacta tcgatcatca cttcgcatct cataccctta cccacaagtt gaaataatag
      421 tattatattg aaaagacaga gattatttca gaggtgttat ggaaaactgg gttattcagg
      481 agctaaaatc actggatata ggggactcaa gacttgataa aagagtaaaa catatcctca
      541 atagcctcag tcgttcacca gaagaaagta ttcctgtttc ctgccgtacc tggagtgaaa
      601 caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa attatggctc
      661 ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta gttttacagg
      721 atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt cacaaaaaac
      781 ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag tcaggtgttt
      841 gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc agtaaaaata
      901 cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa aaagaaacct
      961 ggcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc cctgataccc
     1021 atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa cgcgcagagc
     1081 agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac agagcaacgc
     1141 tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg gagattacac
     1201 cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc agaacactgc
     1261 ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac tccagtaagg
     1321 tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga cacaatggta
     1381 aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc tgttttcgat
     1441 accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt agcgagccag
     1501 cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc gggtgttgcc
     1561 agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag ctgttcggag
     1621 agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag ggccaccgga
     1681 gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac tgcggacaga
     1741 cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtgacgaa ctggcgagta
     1801 agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa
     1861 agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg tgtgggctgt
     1921 ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggtgc tgccatcagt
     1981 agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag cggcccgaat
     2041 agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg attcccttct
     2101 ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac ggtcagcccg
     2161 taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat ggcgctggta
     2221 gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc tcccagagcc
     2281 cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca cggggacgat
     2341 ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag actcagattt
     2401 gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat accgagaagc
     2461 ccggcactga acgcaagtgg ccagcaggac agtgtttttt ggggcgtatc ttcgatccct
     2521 ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa aattaattta
     2581 tctgacaata acatttattg ttgataatga gaatcattat cgacataatt gttattattt
     2641 tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc acagatgtgg
     2701 ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa ctgacagaat
     2761 acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat ttcagagtgg
     2821 cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg gtcacggcat
     2881 caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat aagctacgcc
     2941 atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc
     3001 atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg cgtgaaaaag
     3061 cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc caccctgcgc
     3121 ctaagtctca tgaaccgttt aaccgacggg aggctgaacg ctacctgcct gatatggcac
     3181 cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc
     3241 atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct cagttactca
     3301 acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg gaatatcttt
     3361 tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac ttaggtgagg
     3421 ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc accagccgcg
     3481 atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact ctgtccgtga
     3541 aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc tggcagatgc
     3601 tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc gggctgctcg
     3661 atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg
     3721 atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca cctgatggcg
     3781 gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc
     3841 aacaggccgc ccatgcatgg gtcgatgcat actgtcagca agtgctaaag ccgctgttta
     3901 cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga
     3961 tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc gcttttatgc
     4021 ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat attttcaccc
     4081 atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact tttgctgtga
     4141 ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct cgtgtacgag
     4201 catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg
     4261 aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat cataacgaga
     4321 acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg caggctcagg
     4381 aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc tgtgaaaaac
     4441 tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg cactgtcccg
     4501 gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc gccgcaccac
     4561 aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag gcgctgacgc
     4621 tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag ttaccgttat
     4681 ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga
     4741 tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt tacgatccac
     4801 gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca gaacgtttca
     4861 cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc tcactggagg
     4921 tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg ctgattgggt
     4981 gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa gaccgcattg
     5041 gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt ggtgaacagc
     5101 aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc
     5161 tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac cagcgcctgt
     5221 tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc ccacataagc
     5281 gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa
     5341 tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga
     5401 gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca ttaacctgcc
     5461 gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt ggattgatgc
     5521 ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga tgcagctaaa
     5581 gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt tgtatgccac
     5641 gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat
     5701 taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat tcgtttttaa
     5761 taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag agtataccaa
     5821 caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc gctgcgataa
     5881 cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt ttacacgctt
     5941 cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctaccgctgc cggtacatcc
     6001 gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgtcg aaggcaggat
     6061 ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac
     6121 caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct acaacacctc
     6181 atgctaccgg ggaatccctg gcagatatat cgctgccgga ccactggctt cacgctggct
     6241 gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga tccttggtga
     6301 accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg ctccctatcg
     6361 ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc tgaaaccgga
     6421 tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc agccgctggc
     6481 aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc aattgttccg
     6541 ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca
     6601 cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga
     6661 cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact ctttgcctca
     6721 ggaggtgcgt gatgccacat cccgtctgag tgcggactac ttaatccatg atttgcagac
     6781 gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc ttggcgtacc
     6841 tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga acaaacatcc
     6901 acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca ttcgcgtggt
     6961 gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca tgctgccgaa
     7021 ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg aatcatgaaa
     7081 aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat tgctgcattg
     7141 tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca tttttcctgg
     7201 catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa agatctggtc
     7261 agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa gcacaaaaag
     7321 ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt ttctgactat
     7381 ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt tgaaaatatt
     7441 gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga atattttgcc
     7501 cgcaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt gaagcatgtg
     7561 acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa
     7621 cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa tgcattacgc
     7681 ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa ttttaacgca
     7741 ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc aggcttctcc
     7801 ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac atcggatggc
     7861 atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt tgaagttctg
     7921 agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaaccct ggaaagtagt
     7981 ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga gagcctggaa
     8041 agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat acttccctca
     8101 ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga tgacttcact
     8161 ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag catgcaaact
     8221 catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg tattcttaat
     8281 cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat tcagtggcgc
     8341 agcggcagcc ggaaaaaacc gcagccggag gctgcttctt taactcacta tacagcaaat
     8401 attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc
     8461 ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc atagtgtctg
     8521 ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg
     8581 ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg gctcagttaa
     8641 tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac atgcgtggcc
     8701 gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc gacagccgac
     8761 aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc ggcgcgacgg
     8821 ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa aaaggtcagc
     8881 cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc agtaaagatc
     8941 acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc ggacgtcttt
     9001 ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc accctgcttg
     9061 ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga accggtacgc
     9121 tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa agtcagggag
     9181 acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg agcagcacac
     9241 catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg catttgatca
     9301 gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag gtttactacc
     9361 gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa caggcgacag
     9421 ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact ctgaacagcc
     9481 aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag cgctttacct
     9541 ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg aacaaccata
     9601 agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg gccttcctgc
     9661 aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc tatcagtata
     9721 ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt gctgccggga
     9781 aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac tttctgttca
     9841 atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat ttttcccagg
     9901 gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt gcagcagtga
     9961 acggccatct ttccctgaca aagagcgtga acgtcagcga cagtaagctg gaaggcgtga
    10021 aagtcgattc ttatgaactg ggctggcgct ttaccggtga caacctgcgg actcaaatcg
    10081 cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg accatcagtg
    10141 tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac ctgatcccgg
    10201 atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg
    10261 gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca gcttacatta
    10321 actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc gacgtaagcg
    10381 atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt agttggcagc
    10441 ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac tataccactg
    10501 tctggggaca gcgtgcacct ctgtactact gcccgggtta cggccctgct tcactgtacg
    10561 actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc tgacggggat
    10621 gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg ttgtttacag
    10681 ccagtataat atacagcctt aacaagatat tccgtctccg gaatggactg ttcaccactg
    10741 aataacatta tttctgtttt atggatcttt gactgaaatt tatcgcatat cctcatttca
    10801 ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc tggcagatcc
    10861 tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt gctgcaatga
    10921 aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta aaaagaggta
    10981 cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc tgtcataaag
    11041 tccggtaatg gagattgtta atacgatggt gcacgatgca ttgtcggtgc ccggcccgga
    11101 ggatgccccg taactacata cggatcaggg ttggcaatat cgaatggcag gctataggca
    11161 aagttaaagc cccatggagt agcacaaaat atgccacaca aaggaaacgg tctgaataac
    11221 gcagtgatgg agaacttctt cggcacactg caaaatcgca tcgtggtcga tcgctaattc
    11281 gccgtgaatc actgaacgat gttccatgcg aacagatatt cgatcatatc gaaatattaa
    11341 acattacccc tccccgcatt gggaaaaaaa aaaacaaaaa acgatcaatt agtattgatt
    11401 cacataatct ctcatcatta atgagaacat tgtcacatat ttacaatata gaactgttac
    11461 ttttagtaag ctgttagtaa ccaactgtta ctaataattt taattcttaa tactttgttc
    11521 attcaataaa tgagttgaga
//
LOCUS       DAJVXP010000123        11427 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVXP010000123 DAJVXP010000000
VERSION     DAJVXP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.123"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(43..384)
                     /gene="bamE"
                     /locus_tag="OQS10_003223"
     CDS             complement(43..384)
                     /gene="bamE"
                     /locus_tag="OQS10_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS2294177.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(533..2194)
                     /gene="recN"
                     /locus_tag="OQS10_003224"
     CDS             complement(533..2194)
                     /gene="recN"
                     /locus_tag="OQS10_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS2294178.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2280..3158)
                     /gene="nadK"
                     /locus_tag="OQS10_003225"
     CDS             complement(2280..3158)
                     /gene="nadK"
                     /locus_tag="OQS10_003225"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS2294179.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3281..3874
                     /gene="grpE"
                     /locus_tag="OQS10_003226"
     CDS             3281..3874
                     /gene="grpE"
                     /locus_tag="OQS10_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS2294180.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3929..5215)
                     /locus_tag="OQS10_003227"
     CDS             complement(3929..5215)
                     /locus_tag="OQS10_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS2294181.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5236..6027)
                     /locus_tag="OQS10_003228"
     CDS             complement(5236..6027)
                     /locus_tag="OQS10_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS2294182.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6194..7555
                     /gene="ffh"
                     /locus_tag="OQS10_003229"
     CDS             6194..7555
                     /gene="ffh"
                     /locus_tag="OQS10_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS2294183.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7804..8052
                     /gene="rpsP"
                     /locus_tag="OQS10_003230"
     CDS             7804..8052
                     /gene="rpsP"
                     /locus_tag="OQS10_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS2294184.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8071..8619
                     /gene="rimM"
                     /locus_tag="OQS10_003231"
     CDS             8071..8619
                     /gene="rimM"
                     /locus_tag="OQS10_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS2294185.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8650..9417
                     /gene="trmD"
                     /locus_tag="OQS10_003232"
     CDS             8650..9417
                     /gene="trmD"
                     /locus_tag="OQS10_003232"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS2294186.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9459..9806
                     /gene="rplS"
                     /locus_tag="OQS10_003233"
     CDS             9459..9806
                     /gene="rplS"
                     /locus_tag="OQS10_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS2294187.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9883..10365)
                     /locus_tag="OQS10_003234"
     CDS             complement(9883..10365)
                     /locus_tag="OQS10_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS2294188.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10381..>11427)
                     /gene="dgcN"
                     /locus_tag="OQS10_003235"
     CDS             complement(10381..>11427)
                     /gene="dgcN"
                     /locus_tag="OQS10_003235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCS2294189.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2747 c         3061 g         2817 t
ORIGIN      
        1 cattgagcac cttttttaac gtctttgaga gcaactttat tattagttac cactcagcgc
       61 aggtttgtta tcaatattgg tcaacacacc gctactgtta aaggtcagcg tcagcgtttg
      121 ctgcgttaca ccttcatgac ctggttgctg gcggaagaca tagaaccagg tattcgtacc
      181 aaatggatcg gacatcagcg gtgtacccaa tgcgtacgca acttgttgtt gcgtcatgcc
      241 aacacgtatt ttggatacgt cgttagcggt cagatagttc ccctggttga tgtcaggacg
      301 gtaaaccact cgctccagag tggaacagcc tgcggtcaac atcaatagta ctgctgctgc
      361 agcagtcagc gttttacagc gcatagtgat ttgattcctt ttcgggcccg agcagtacgt
      421 ggctcatctg taatatgccg atgataatag acctttcatc actttaaaac cttttgcttt
      481 ccggtcttac ggcgttttgc tgtttactct aaccgtgaag caggaaaaaa gtttacgctg
      541 caagcagttc tttcgcattc gctagtgtat tacgtgtgac ttcactgcca ccaagcaggc
      601 gcgccagctc ttgtaaccgc gcttttttat tcagggattg catatgcgtt tctgtcatcg
      661 caccatcggt ttctttgctg acaaaatagt gttgatgacc acatcccgcg acttgtggca
      721 ggtgggtaac acacatcacc tgagttgatt cgccgagttg acgcagcagt ttgccgacaa
      781 ctgccgctgt tggaccgcta atccctacat ccacttcatc aaaaatcagt gccggggttt
      841 ccattttacg cgccgtgatg acctggattg ccagtgcgat gcgggacaat tcaccaccgg
      901 atgcgacttt ggcaataggc tgcattggct gacctgggtt ggtggttacc cgaaactcaa
      961 tacgatcggc accgtcagcg cccaggtgat gctcgtcaaa tttaacatcg atcgtaaact
     1021 gcccatgcgg cattgagagc gcatgcatac tgtcggtgat cagctgtgca agttcttctg
     1081 catattgctg gcgttgttgg tgtaatgcgc gcgcgatttc cagtgcctgc tgatgatgtt
     1141 tcgttaccgc cagcgcaagc gtttcttgtg agtcggcctg atcgtccagt tgctgctgtt
     1201 cttccagtag cgactggtaa tactgtggca atgcctcagg gctgacgtga tgtttacgtg
     1261 ccagcgaaat ctgttttgag atgcgctgtt caagttcaaa tagtcggttg ggatcgagat
     1321 ccagacgatc gcagtagtgg cgcagttcat cgctggcttc agcaatctgg atggtagctt
     1381 cttccagcat atcaagtacg ccggacagtt tgctgtccat gccaatcaat tcgctcacca
     1441 gttgtttagc cgtgtaaagc tgactttgca ggtttgcgtc ttcaccgtcg gccattaatg
     1501 ccaatgcatt ctggctggtg gtcagcaatt gaccgctgtt cgccagacgt ttgtactctt
     1561 cgtcgatttg ttcaaactct ccgggctgcg gattaaattc gttaagttct tttaattggt
     1621 attgcagcag ttccgcacgg gcggcgcgtt cctgacttaa ctgttgatga tgcgcgaggt
     1681 cacggcagct ttgatgccac aactgataac gtgcggtcat ttcctgcagt agagaggttt
     1741 cattggcata gccatcaagc aggaattttt ggtgctcagg tttggtgagt aattgatgag
     1801 cgtgctgacc atggatctga atcagcaact gacccagttc gcgcagttgt gacagaggaa
     1861 cagctgtacc gttgatgaaa ccacgggagc gaccatcgct gctgatcacg cgacgaagca
     1921 aacattcatg cccgtcttca agctggtttt cttccagcca gcgcagagcc gctggcgtat
     1981 ctttcagaga aaaacgggcg cacaggtcag cgcgagcagc gccggtacgc accatgtcgg
     2041 cttcagcgcg accaccgaga caaagaccga gggcatctat tgcaatagat ttacccgcgc
     2101 cggtctcgcc agttattacg gtcatgccgc tatgaaaatc aatctcaagc tcacgaacga
     2161 tagcaaagtt gctgatggtc agttgtgcca acatagtcgt tttcctgtat gaaaaaccat
     2221 tactgttatt gtgtacagta taaactggtt ttatatacag taaagaggct ggcgtaaaat
     2281 tagaataatt tttttgacca gccgagcttg gtgcttaatg tgttgaaata actgtaatct
     2341 ttcggatgaa tcagattcag atggtaatca cagcgacgaa tcaggacatc ttcaccttcc
     2401 tgaatcggca gtgctatctg gctgtcgcaa ctgatttcca ggtcgttacg gcgatgcgaa
     2461 aaacgcagac ggatcgtgct gctgctgttt atgaccagtg gtcgtgctga caacgtatgc
     2521 gggaacatgg gcaccagggt aatcgcatcc agagaggggg tcagaatagg accgcctgca
     2581 gagagggaat aggcggtgga gcctgttggc gtcgaaataa ttagtccatc agatcgctga
     2641 gaaaacgcaa agatctcgtc gatatacact tcgaactcaa tcatatgcgc cactttgcct
     2701 ggatgaagca ccacttcatt tatcgcggtg ctgatgcgtt tctggcaatc ttgctgacag
     2761 acttgcgctt ccagcaaaaa acgtttctcg ctgatgtagt ggccttccag cacatcggct
     2821 aactgttgct gggcgttatc ggggtcaagg tcagtcagga aacccaggtt gccacggttg
     2881 attccaataa ctttaatatc gtaacgggcg agtgtgcgtg ccgcgcccag catattaccg
     2941 tcgccaccaa cgactaccgc gagatcagct agttgcccaa tctccgcgag cgtgccagtt
     3001 ttcacattct tcagttgcag ttcgtgagcg atttgttgct caacgatgac ctcgtaacct
     3061 tttgtgcaca gccagcggta gagcatttca tgtgttgtca gtgcagtggg gtgccgtggg
     3121 tgtcccacaa tgccaataca cttgaaatga ttattcattt ttccgaggtc cttgttgcga
     3181 agattgatga caatgtgagt gcttcccttg aaaccctgaa actgatcccc ataataagcg
     3241 aagttagcga gatgaatgcg aaaaaaacgc ggagaaattc atgagtagta aagaacagaa
     3301 aacgcctgag gggcaagccc cggaagaaat tatcatggat cagcacgaag agattgaggc
     3361 agttgagcca gaagcttctg ctgagcaggt ggatccgcgc gatgaaaaaa ttgcgaatct
     3421 cgaagctcag ctggctgaag cccagacccg tgaacgtgac ggcattttgc gtgtaaaagc
     3481 cgaaatggaa aacctgcgtc gacgtactga actggatatt gaaaaagccc acaaattcgc
     3541 gctggagaaa ttcatcaacg aattgctgcc ggtgattgat agcctggatc gtgcgctgga
     3601 agtggctgat aaagctaacc cggatatgtc tgcgatggtt gaaggcattg agctgacgct
     3661 gaagtcgatg ctggatgttg tgcgtaagtt tggtgttgaa gtgatcgccg aaactaacgt
     3721 gccgctggac ccgaatgtgc atcaggctat cgcaatggtg gaatctgatg acgttgcgcc
     3781 aggtaacgta ctgggcatta tgcagaaggg ttatacgctg aatggtcgta cgattcgtgc
     3841 ggcgatggta actgtagcga aagcaaaagc gtaatttctg ttttcgtaat aattcacggc
     3901 cctgcatgcg aatgcagggc cgttttcgtt actccgccac actctcgcgc agcggtttca
     3961 caggaaaaac ttttacctgc ttaatcatat tgtcctgtac gtcgagaata tctatatcgt
     4021 actcgccaat acgcacgcgg gtgcctgcga cagggatctc ttccagtgcc tcaagaatga
     4081 cgccattaac cgtgcgggca tcatcttccg gtagatgcca gttaaaggct ttgttgattt
     4141 cccgcacgtt ggcggtgcca tcgataatca ccgaaccgtc gttttgcggc gtgacctctt
     4201 cggcaagtgt tggcgacatc gacgtagtga aatcgccgac aatctcttcc agaatatctt
     4261 caaccgtcac cagcccctga atgtctccat actcgttgac gaccaggccg actttctttt
     4321 tgttgcgctg aaactttacc aactgcgtgc tgagcggcgt accttccggc acaaaataga
     4381 tctcgtccgc ggcgcgcagc atggtttctt tggtgaactc ttttttctcc gacatcaacc
     4441 gccaggcttc acgtactcgc agcatactga tggcgtcgtc cagcgaatca cggtagagca
     4501 cgatgcgccc gtgaggtgag tgggagagtt ggcgcagaat cgatttccag tcatcgttga
     4561 tatcaatacc gataatttca ctgcgcggca ccatgatgtc atcaacggtc attttttcca
     4621 gatcgagcac cgacagcagc atatcctgat tgcgacggga aatttgtgag cgtgattcgt
     4681 gcacgatagt gcgcaactct tctttgctca aagagccgct aaccacgata tcggttttga
     4741 tacccatcat gcgcatcagc atacgggtga tagcattcag caaccagacc agcggcatca
     4801 tcaaaatttg cagcggagcc agcagaaaac tactcggata agcgactttt tccgggtaca
     4861 gcgcggcaat ggttttcggc aatacctcag caaataccag tacgacaaaa gtcagcacac
     4921 cagtcgcaat tgccacgccc gcatcgccgt acaaacgcat cccaacaata gtgccgagcg
     4981 cggaggcaag aatattgacc aggttattgc cgattaacac caggcttatc aggcggtctg
     5041 gcttacgcag caatttttcg acgcgtttgg ccgagcgatt accctgtttc gccatatgtc
     5101 gcagacgata gcggttgagg gtcatcattc cggtttcgga cccggaaaaa taggctgaaa
     5161 tgaccaccat gatgatcaga ataatgatca acgtagtagt agaaatgtgt tccaggggaa
     5221 actccttttc tgggtttagc tgattaactg ctggacaatt cggctgccga agtaggccag
     5281 tgtcagaatt accgcgcccg caacgttaaa ccagacgacg cggcgtccac gccatccttc
     5341 atggtaatgt ccccacagca gcacaatata gacaaaccac gccacgatag agagcacagc
     5401 cttgtcgata ttttccatgc taaataggtt gtgcatgtag aacaggccag tgcaaagcgt
     5461 gagcgttagc agcaccacgc caatctgcgt gatgtggaac attttacgct cgatactcat
     5521 caatggcggc atttcctggt taaatgccag cttcttgttc ttcagttggt aatcaatcca
     5581 cgccagttgc agcgcgtaca gggcggcgat aattagcgtg gcataggaaa agagcgataa
     5641 gccaatgtgc accagcatcc caggcgttgc ttccagatgg gtgatgtatt cattgggcat
     5701 gaaggtcgcc agcgccaggt tgataagcgc aaaggcatag acaatgggta gcagcaacca
     5761 gccacgattg cgagaagcca caatggtcat taccgtacag atcatcaaac tgaccaatga
     5821 accaacgttc agcaggctga ggttttgtcc gctatcaccg tcgggcagga tgcgggcttc
     5881 cagagcgatt gcgtggcaga ccagcgcaat gaccgcagaa ataatagcca tgcgccgcca
     5941 gccgccgttt ttttgcagca gaccgggaac aatcagcgca agactgacgg agtaggcgac
     6001 aagcgcgagc agagcaaaaa cgggcatagt gatgtcgaca gtttgagcaa taagaaagag
     6061 aagcagtata gcgtcaggtg aacgctgctc caaccgttgc ataacaacaa agacgccttc
     6121 atgttatact gcggcaaaat actgatgatg tgtcgcgatt gcggccaacc gtttccaccc
     6181 caggcgagag acaatgtttg ataatttaac cgatcgtttg tcgcgcacgc tgcgcaatat
     6241 cagtggccgt ggacgcctca ctgaagacaa cgtaaaagat acgctgcgcg aagtgcgcat
     6301 ggcgctgctg gaggcggatg tcgctctgcc ggtagtgcgt gagtttatca atcgcgtaaa
     6361 agagaaagcg gttggtcatg aagttaataa gagcctgacg ccggggcagg agttcgtcaa
     6421 aatcgtccgt aacgaactgg ttgcggcgat gggcgaagag aaccagaccc tgaacctggc
     6481 ggcgcaaccg cctgcggtcg tgctgatggc gggcctgcaa ggtgccggta aaacaaccag
     6541 cgttggtaaa ctcggtaagt tcctgcgcga gaagcacaag aagaaagtgc tggtggtttc
     6601 tgccgacgtt tatcgcccgg cggcaatcaa acagcttgag acgctggcag agcaggtggg
     6661 cgttgatttc ttcccttctg atgttggtca gaagccggta gatatcgtta acgcggcgct
     6721 gaaagaagcc aaactgaaat tctacgacgt gctgctggtg gataccgctg gtcgtctgca
     6781 cgttgacgaa gcgatgatgg acgagatcaa acaagtccat gcgtcgatta acccggttga
     6841 aaccctgttt gtggttgacg ccatgaccgg tcaggatgcg gccaatacgg caaaagcatt
     6901 caatgaagcg ttaccgctta ccggcgtagt gttgaccaaa gtggacggcg atgcccgcgg
     6961 cggtgcggcg ctctctattc gtcacatcac tggcaaaccg atcaagtttc tcggtgttgg
     7021 cgagaaaact gaggcgctgg agccgttcca tccggaccgc atcgcgtcgc gtattctcgg
     7081 catgggcgac gtactgtcgc tgatcgaaga tatcgaaagc aaagttgacc gcgcgcaggc
     7141 agagaaatta gccagcaagc tgaaaaaagg cgacggcttc gatctcaacg attttcttga
     7201 gcagctgcgc cagatgaaaa atatgggcgg catggctagc ctgatgggca agctgccggg
     7261 catggggcag atcccggata atgttaagtc gcagatggac gataaagtgc tggtgcgtat
     7321 ggaagccatc atcaactcga tgacgatgaa agagcgcgct aagccagaaa tcatcaaagg
     7381 ttcgcgtaag cgtcgtatcg ctgccggttg cggtatgcag gtgcaggacg ttaaccgcct
     7441 tctgaagcag ttcgacgaca tgcagcgcat gatgaagaaa atgaagaagg gcggaatggc
     7501 gaagatgatg agaagcatga agggtatgat gcccccaggc ttccctggtc gctaatcggc
     7561 ctggcttgtt tgccattttg gcaccgggat gtgctcgtca tcctcacgta ctgcgtgtac
     7621 gctccggtga ctgcgcgcac tccggcacca aactggctgc accgccgacg gccttttaca
     7681 gcagagttag caactgttga ttgcaattcc cgcaaaaatg agtaaaattt tcgggctttt
     7741 aatatgacac cggactccgt tcctcgatgg ggtccggttg ttttattcac acaagaggat
     7801 gttatggtaa ctattcgttt agcacgtcac ggcgctaaaa agcgtccgtt ctaccaggtt
     7861 gttgtcgctg acagccgtaa tgcacgcaac ggtcgcttca tcgagcgcgt tggtttcttc
     7921 aacccaatcg ctagcgaaaa agaagaaggc actcgcctgg atctggatcg catcgctcac
     7981 tgggttggcc agggcgcaac tatttctgat cgcgttgctg cgctgatcaa agaagtaaac
     8041 aaagcagctt aatctgtcac ggtggtcatg atgagcaaac aactcaccgc gcaagcacct
     8101 gttgatccca tcgttttggg aaaaatgggt tcgtcttacg gtattcgtgg gtggctcaga
     8161 gtgttttctt ccaccgaaga cgccgaaagc atttttgact atcagccctg gtttatccag
     8221 aaggcgggtc agtggcagca agtccagctg gaaagctgga agcaccacaa tcaggacatg
     8281 atcatcaagc tgaaaggcgt tgacgatcgt gatgcggcga acctgctgac gaattgtgaa
     8341 attgtcgtgg attcatcgca gctgcctcag cttgaagagg gggactacta ctggaaagac
     8401 ctgatgggct gccaggtagt aaccactgaa ggctacgatc tcggtaaagt cgtcgatatg
     8461 atggaaaccg gatctaatga cgttctcgtc attaaggcaa acctgaaaga tgcgtttggt
     8521 atcaaggaac gtctcgtacc gttcctcgat gggcaggtta tcaagaaagt cgatctcact
     8581 actcgttcaa tcgaagtaga ttgggatcct ggtttttaaa ccaccggata aacggtaaaa
     8641 gacggcgcta tgtggattgg cataattagc ctgtttcctg aaatgttccg cgcaattacc
     8701 gattacgggg taactggccg ggcagttaaa aatggcctgc tgagcatcca gagttggagt
     8761 cctcgcgact tcacgcatga ccggcaccgt accgtggacg atcgtcctta cggtggcgga
     8821 ccggggatgt taatgatggt gcaacccttg cgggacgcca ttcatgcagc aaaagccgcg
     8881 gcgggtgaag gcgcaaaggt gatttatctg tcaccacagg gacgcaagct tgatcaagcg
     8941 ggcgtcagcg aactggcaac gaatcaaaaa ttgattctgg tgtgcggtcg ctacgaaggt
     9001 atagatgagc gcgtgatcca aaccgaaatt gacgaagaat ggtcaatcgg cgattacgtt
     9061 ctcagtggtg gtgagttacc agcaatgacg ctgattgact ccgtttcccg gtttattccg
     9121 ggagtactgg gacatgaagc ctcggcaacg gaagattcct ttgctgaagg attgctggat
     9181 tgcccgcact atacgcggcc tgaggtgtta gaagggatgg aagttccgcc agtgttactg
     9241 tcgggcaacc atgccgagat acgtcgctgg cgtttgaaac agtcgctggg ccgtacctgg
     9301 cttagaagac ctgaacttct ggaaaacctg gctctgactg aagagcaagc aaggttgctg
     9361 gcggagttca aaacggaaca cgcacaacag caacataaac atgatgggat ggcgtaagcc
     9421 cccgagatat cagtttaccc aggataagag attaaattat gagcaatatt attaagcaac
     9481 ttgaacaaga gcagatgaag caggacgtac cttccttccg tccgggtgat accgtggaag
     9541 tgaaagtatg ggttgttgaa ggttccaaaa aacgtctgca ggcattcgag ggcgtggtta
     9601 tcgctattcg taaccgcggt ctgcactctg cattcactgt tcgtaaaatt tccaacggcg
     9661 aaggcgttga gcgtgtcttc cagactcact ctccggtagt tgacagcatt tctgtcaaac
     9721 gtcgtggtgc tgttcgtaaa gctaaactgt actacctgcg tgagcgtact ggtaaggctg
     9781 ctcgtatcaa agagcgtctt aactaagatt tcgcttaagc gacatcctgt taagaagggc
     9841 tggccaattg gctggccctt tttttgtctg tttgctcgtg ttttaagggg ttgtaatcac
     9901 cactgcgacg cggcggttct cggcgcggcc ctgggcggtc ttgttactgg ctatgggata
     9961 tttttttcct aaaccctgtg tggtgagatt gctgcgtgga atttgtccac caatagccca
    10021 tgcatcggcc acgacattcg cccgtttcaa tgataagcct tcgttgtaac tgtcttcacc
    10081 atagttatcg gtgtgtccat ccatacgggc atgtgttagc cctgtcgagg ccaatttggc
    10141 tgccatggtt tggatctgtt gctggctttc cgggagcaat ttgtagtcat tttttgcgaa
    10201 cagaatggca tctgataagc ccagcgacca gtcgccggcg gattcagtaa atccataaga
    10261 ttgcatagcg gcgacttgct caggagtaaa ctttccctga ggggactggc agccagtcag
    10321 tattagtgag gtgaaaatca ggggtgctac taggtgcttt atcatatcga tatatccttg
    10381 ttatctcacc agcttttcgg cacgctggtg tttggcctga tacatattgt gatcggcaag
    10441 ctcttgtaat ttttccgcag aggcgtgctc aatggtcatc gcgtaaccaa tgcttaatgt
    10501 catggtggtc tgatggccat tatgaagatc aaacgggaga ttaaagattt gtgtcagtgc
    10561 tgagcatatc tgctgcactt cagattctga ctgtacatca tagagcacca tagcgaattc
    10621 atcgccgccc aggcggtatg ctttatgtcg cagcccgcca acttcagcta accgttttgc
    10681 gatttcaatc aagactctat cgcccgtcgc atgaccccag gtatcattga tgtatttgaa
    10741 attatcgcca tcaagaaata gtaacgccga cgtttttcgg gcatcggaat tgttcatcaa
    10801 cgtgttgatg ccgctacgaa acgctgcgcg gttagccagc ccggttaatg ggtcatgtag
    10861 cgcggtacgt agaagctgcg catttttagc ctgtaaacga agctgccact cttccatttc
    10921 atccagcaga ctgttgaagt cgagagcgaa gcggtgaaac tcagcgatac gttcttccga
    10981 aactcgtcgg gaaaaattgc ggttggaacg cacatcatgt acgacatcgg tgatattttt
    11041 cagtgcttcc accaggccat tgtgcaaatg gcgggtgagg gtaattgcga tgcctgatgc
    11101 cagcagaata caaccggtca gtacggcgag cgaaaaccag ataaaatggc tgattgaact
    11161 gtcgcgagcg gttaagcgta cttcgccaat ggtttcacca ttgtgacgaa tcggctgaat
    11221 gatgggggcg gggaagagcc agtggcttat gaaattgctg aaagtatcgc ctggatcctt
    11281 acgggtgtaa tgccaggacg ccagaatatt ttgctgctta tcacgtactt ctgcagttga
    11341 aaattgccct tgctggccca gcgctgccag tgtttcagtt gctgcagggc catcggcaaa
    11401 aacgacagct gcttccagac tgtaagt
//
LOCUS       DAJVXP010000124        11311 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVXP010000124 DAJVXP010000000
VERSION     DAJVXP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.124"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..210
                     /locus_tag="OQS10_003236"
     CDS             <1..210
                     /locus_tag="OQS10_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2294190.1"
                     /translation="KIVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMF
                     AQTTKGVLETGIYRGVVSIYLSQDI"
     gene            302..2986
                     /locus_tag="OQS10_003237"
     CDS             302..2986
                     /locus_tag="OQS10_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS2294191.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2983..4071
                     /locus_tag="OQS10_003238"
     CDS             2983..4071
                     /locus_tag="OQS10_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS2294192.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="OQS10_003239"
                     /pseudo
     CDS             complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="OQS10_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5934..7346
                     /gene="uxaC"
                     /locus_tag="OQS10_003240"
     CDS             5934..7346
                     /gene="uxaC"
                     /locus_tag="OQS10_003240"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS2294193.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7361..8848
                     /gene="uxaA"
                     /locus_tag="OQS10_003241"
     CDS             7361..8848
                     /gene="uxaA"
                     /locus_tag="OQS10_003241"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS2294194.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8931..9482
                     /locus_tag="OQS10_003242"
     CDS             8931..9482
                     /locus_tag="OQS10_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS2294195.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="OQS10_003243"
     CDS             complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="OQS10_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS2294196.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2971 a         2773 c         2934 g         2633 t
ORIGIN      
        1 aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc
       61 acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa
      121 aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta ccgtggtgta
      181 gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca cgtaacaaag
      241 ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt tcaggattgc
      301 catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg cagaggcgac
      361 attaaccgaa attcccgcag ggtttgaggt tattgctcag ggacagcagg agtatatcga
      421 ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc ttgataccgt
      481 aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag acgatcagaa
      541 aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcaccacg gtgaattggc
      601 ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata cgctggaaat
      661 aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat ggaattcagc
      721 tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg cttttataca
      781 tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta ttcagggaaa
      841 cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt tcaacggtta
      901 tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt atcgttatga
      961 ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac tatttaatgc
     1021 acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg ggatgcgtat
     1081 cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt
     1141 tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt
     1201 ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag gcagctacag
     1261 cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac
     1321 caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac
     1381 atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca
     1441 tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt
     1501 aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca gcgccagcgt
     1561 gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg
     1621 atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag
     1681 cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg tttcacagaa
     1741 ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca ctgatgatag
     1801 tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga caacagcgca
     1861 aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc gtgactggat
     1921 tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa
     1981 cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag
     2041 ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg atcaaacgtc
     2101 atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac
     2161 cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca
     2221 atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct
     2281 ttcggccttt acgggcactt atagctctac gctggctgtc agttgctatg gcgttaattt
     2341 gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc
     2401 tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac
     2461 tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt
     2521 tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg
     2581 tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc
     2641 cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc
     2701 gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggaggat ttacacttga
     2761 aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc
     2821 actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt
     2881 ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa
     2941 attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt
     3001 gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc
     3061 agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa
     3121 gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa
     3181 acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt
     3241 gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa
     3301 gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga
     3361 tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc
     3421 taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt
     3481 aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc
     3541 gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag
     3601 ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg
     3661 ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc
     3721 aaagatgaag ggcgaccagc tgcagggcgc agagacggtg acttctctat ctatcgtcaa
     3781 ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg
     3841 gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac
     3901 ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg
     3961 acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac
     4021 atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaattg accccgcaga
     4081 ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc ttcgtcatta caacttacgt
     4141 taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     4201 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     4261 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     4321 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     4381 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     4441 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     4501 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     4561 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     4621 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     4681 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca
     4741 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4801 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4861 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4921 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4981 ttgccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     5041 gcctactgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     5101 cgggatcatc gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca
     5161 gctacctgcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     5221 accgattttc gtacccagca catccagtac ataacctgct accggttgca tgaccgtata
     5281 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     5341 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     5401 gagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     5461 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     5521 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     5581 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     5641 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     5701 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5761 aggccagtac tggcgcggcc tgcagcgaga tttatcactt tgagagtaat ttttttaact
     5821 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     5881 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     5941 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     6001 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     6061 aagactatcg ttttaaaaac ctgtatgaca tctggctgaa aggcgatcac tacaaatggc
     6121 gcgctatgcg taccaacggt gtggccgagc gtctgtgtac cggtgatgca tctgaccgtg
     6181 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     6241 ggacgcacct tgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa
     6301 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     6361 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     6421 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     6481 gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga
     6541 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     6601 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     6661 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     6721 ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     6781 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     6841 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     6901 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     6961 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     7021 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     7081 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     7141 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     7201 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     7261 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     7321 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     7381 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     7441 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     7501 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     7561 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     7621 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga
     7681 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct
     7741 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     7801 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc
     7861 acaactgggc gacgatcaca tcaacatccg caccatgctg caaaacatgg tgcgccaccc
     7921 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     7981 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     8041 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     8101 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     8161 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     8221 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     8281 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     8341 ctttaaacag tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa
     8401 caaagcgggc ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg
     8461 ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc ctgggctgaa
     8521 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     8581 ccatatggtg ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt
     8641 gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     8701 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     8761 cgttgagttt gccaacggta agcaaacttg caacgagcgt aacgacttcc gcgaactggc
     8821 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     8881 ttcataatgt gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt
     8941 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
     9001 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
     9061 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
     9121 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
     9181 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
     9241 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
     9301 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacaatga
     9361 ttgagggtag ttttctgctt atgaatggct tgaatatcat tcgtttctgg cggatgcaga
     9421 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccgtagac gaaaggggtt
     9481 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
     9541 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
     9601 gccgatgata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
     9661 attacaggcc agcgggatca gcagcagaga accccccgcc acgccggatg cgccacaggc
     9721 acacagagaa gccaccacgc tcaacagcag cgccgtgggc agatcgaccg ggatacccag
     9781 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat
     9841 ggtggcaccc agcggaatag aaacggaata ggtatcgcga tccagattca gcttttcaca
     9901 cagtgccata ttcaccggaa tgttcgccgc agagctgcgg gtgaagaagg catacacgcc
     9961 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
    10021 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
    10081 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagacc
    10141 aaaaatacca atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga
    10201 catatcgttg accaggtttt tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc
    10261 aattgcccac accaggatcc cgatgtagtt acctttcagc agcgcgtcga tggggttgga
    10321 aaccatgctc attaccagcc cgcgcatcac ttctacaatg cctgacggtg gcgaaatatc
    10381 acccgcgcta ctggataagt gcagggtaga agggaaggcg aagctgaaaa cgactgcggc
    10441 cagagcagca gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt
    10501 ctgcccatgc tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc
    10561 aacggctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    10621 ttttgatatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    10681 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    10741 ctttcattgt gttatcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    10801 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    10861 gattttaaag agaagtcata ttttgaaact taatggttac atttgtttga cataaacaga
    10921 gggaaggact ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg
    10981 agaacggttg cgctccttta atgtgctatg ccatataaag tcactggcgc agggagcgcg
    11041 cagggggcgg ccaatcgccg ccgccccctg cacccccagg ctctggcgaa caaaatcgcc
    11101 gctgcgcgat tccctcagct tatcccttgc ggctaccggg tcgggcgcaa gggaacgtcc
    11161 ctgtaagatg cgccctcgcc gcgcatccat gcgcgtcgcc ccggcctcca gggaacgctt
    11221 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta caagccgatt
    11281 acataattaa tagctcacct ttgtctggaa t
//
LOCUS       DAJVXP010000125        11292 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVXP010000125 DAJVXP010000000
VERSION     DAJVXP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.125"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..611)
                     /locus_tag="OQS10_003244"
     CDS             complement(<1..611)
                     /locus_tag="OQS10_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS2294197.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="OQS10_003245"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="OQS10_003245"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS2294198.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="OQS10_003246"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="OQS10_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS2294199.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="OQS10_003247"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="OQS10_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS2294200.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="OQS10_003248"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="OQS10_003248"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS2294201.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /locus_tag="OQS10_003249"
     CDS             6897..7127
                     /locus_tag="OQS10_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS2294202.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="OQS10_003250"
     CDS             7139..7411
                     /locus_tag="OQS10_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2294203.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="OQS10_003251"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="OQS10_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS2294204.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="OQS10_003252"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="OQS10_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCS2294205.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="OQS10_003253"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="OQS10_003253"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS2294206.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..>11292)
                     /gene="dtpC"
                     /locus_tag="OQS10_003254"
     CDS             complement(10008..>11292)
                     /gene="dtpC"
                     /locus_tag="OQS10_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCS2294207.1"
                     /translation="VTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         2914 a         2744 c         2773 g         2861 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cg
//
LOCUS       DAJVXP010000126        11197 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVXP010000126 DAJVXP010000000
VERSION     DAJVXP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.126"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1225
                     /gene="narU"
                     /locus_tag="OQS10_003255"
     CDS             <1..1225
                     /gene="narU"
                     /locus_tag="OQS10_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS2294208.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1307..5047
                     /gene="narZ"
                     /locus_tag="OQS10_003256"
     CDS             1307..5047
                     /gene="narZ"
                     /locus_tag="OQS10_003256"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCS2294209.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5044..6588
                     /gene="narH"
                     /locus_tag="OQS10_003257"
     CDS             5044..6588
                     /gene="narH"
                     /locus_tag="OQS10_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS2294210.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6588..7283
                     /gene="narW"
                     /locus_tag="OQS10_003258"
     CDS             6588..7283
                     /gene="narW"
                     /locus_tag="OQS10_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS2294211.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7280..7960
                     /gene="narI"
                     /locus_tag="OQS10_003259"
     CDS             7280..7960
                     /gene="narI"
                     /locus_tag="OQS10_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS2294212.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8039..8932
                     /locus_tag="OQS10_003260"
     CDS             8039..8932
                     /locus_tag="OQS10_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCS2294213.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="OQS10_003261"
     CDS             complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="OQS10_003261"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCS2294214.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10046..10615
                     /locus_tag="OQS10_003262"
     CDS             10046..10615
                     /locus_tag="OQS10_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCS2294215.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="OQS10_003263"
     CDS             complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="OQS10_003263"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCS2294216.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10946..>11197)
                     /locus_tag="OQS10_003264"
     CDS             complement(10946..>11197)
                     /locus_tag="OQS10_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294217.1"
                     /translation="ATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHI
                     AINILTNDKVFKAGLRRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2599 a         2923 c         2990 g         2685 t
ORIGIN      
        1 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
       61 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      121 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      181 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      241 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      301 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      361 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      421 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      481 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      541 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      601 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      661 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      721 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      781 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      841 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      901 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
      961 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1021 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1081 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1141 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1201 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1261 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1321 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1381 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1441 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1501 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1561 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1621 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1681 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1741 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1801 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1861 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1921 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     1981 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2041 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2101 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2161 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2221 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2281 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2341 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2401 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2461 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2521 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2581 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     2641 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2701 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2761 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2821 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2881 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     2941 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3001 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3061 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3121 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3181 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3241 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3301 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3361 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3421 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3481 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3541 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3601 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3661 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3721 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3781 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3841 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3901 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     3961 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4021 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4081 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4141 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4201 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4261 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4321 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4381 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4441 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4501 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4561 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4621 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4681 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4741 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4801 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4861 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4921 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     4981 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5041 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5101 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5161 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5221 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5281 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5341 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5401 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5461 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5521 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5581 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5641 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5701 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5761 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5821 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5881 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     5941 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6001 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6061 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6121 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6181 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6241 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6301 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6361 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6421 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6481 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6541 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6601 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6661 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6721 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6781 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6841 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6901 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     6961 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7021 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7081 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7141 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7201 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7261 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7321 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7381 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7441 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7501 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7561 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7621 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7681 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7741 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7801 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7861 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7921 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     7981 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8041 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8101 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8161 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8221 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8281 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8341 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8401 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8461 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8521 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8581 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8641 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8701 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8761 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8821 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8881 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     8941 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9001 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9061 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9121 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9181 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9241 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9301 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9361 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9421 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9481 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9541 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9601 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9661 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9721 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9781 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9841 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9901 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
     9961 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10021 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10081 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10141 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10201 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10261 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10321 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10381 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10441 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10501 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10561 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10621 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10681 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10741 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10801 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10861 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10921 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    10981 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11041 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11101 ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc caatgcagct
    11161 tattctccac gtgccagtgg tttcggattg ctgtggc
//
LOCUS       DAJVXP010000127        11058 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVXP010000127 DAJVXP010000000
VERSION     DAJVXP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.127"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            329..1000
                     /locus_tag="OQS10_003265"
     CDS             329..1000
                     /locus_tag="OQS10_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCS2294218.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1010..2206
                     /locus_tag="OQS10_003266"
                     /pseudo
     CDS             1010..2206
                     /locus_tag="OQS10_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2206..2766
                     /locus_tag="OQS10_003267"
     CDS             2206..2766
                     /locus_tag="OQS10_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS2294219.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2768..3541
                     /locus_tag="OQS10_003268"
     CDS             2768..3541
                     /locus_tag="OQS10_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCS2294220.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3729..4004
                     /gene="frmR"
                     /locus_tag="OQS10_003269"
     CDS             3729..4004
                     /gene="frmR"
                     /locus_tag="OQS10_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCS2294221.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4039..5148
                     /gene="frmA"
                     /locus_tag="OQS10_003270"
     CDS             4039..5148
                     /gene="frmA"
                     /locus_tag="OQS10_003270"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCS2294222.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5241..6074
                     /gene="fghA"
                     /locus_tag="OQS10_003271"
     CDS             5241..6074
                     /gene="fghA"
                     /locus_tag="OQS10_003271"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS2294223.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6300..6839)
                     /locus_tag="OQS10_003272"
     CDS             complement(6300..6839)
                     /locus_tag="OQS10_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCS2294224.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6941..8152)
                     /gene="mhpT"
                     /locus_tag="OQS10_003273"
     CDS             complement(6941..8152)
                     /gene="mhpT"
                     /locus_tag="OQS10_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCS2294225.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8439..9452)
                     /gene="dmpG"
                     /locus_tag="OQS10_003274"
     CDS             complement(8439..9452)
                     /gene="dmpG"
                     /locus_tag="OQS10_003274"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCS2294226.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9449..10399)
                     /gene="mhpF"
                     /locus_tag="OQS10_003275"
     CDS             complement(9449..10399)
                     /gene="mhpF"
                     /locus_tag="OQS10_003275"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCS2294227.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10396..>11058)
                     /gene="mhpD"
                     /locus_tag="OQS10_003276"
     CDS             complement(10396..>11058)
                     /gene="mhpD"
                     /locus_tag="OQS10_003276"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCS2294228.1"
                     /translation="VVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEI
                     IPFSRVLQPRIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQF
                     VDTVADNASCGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAV
                     WLARKMASLGEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGS
                     LS"
BASE COUNT         2703 a         2959 c         2719 g         2677 t
ORIGIN      
        1 tcaatcactc gcatattaca ttcacataat gtatataaac aatacttgtt cacagaacat
       61 attttttaaa tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa
      121 aataactata cataccaaac atacaaacac ttaacatatg aattgcataa aaacattacc
      181 cagtcgaacc tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt
      241 ataaattaac atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag
      301 tataataaat aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcctttc
      361 ttcggcaaat aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt
      421 aggctgtggc gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagcagtggt
      481 gatgactacc ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa
      541 cgccctaaag atattagggt gcaaccaaac tattcatctt aattttgctg acacccgcgc
      601 tcatttacag ctcaatgata tgatttccgc cctggaagac atcattaaaa atcaaattcc
      661 ttctgatgtt gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca
      721 tctcgctgtt tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta
      781 cgaaacccca agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga
      841 atatttcaca gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta
      901 tatgcgtcat gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga
      961 tctgggtgaa ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg
     1021 gatatttatc tgtatgtcca tagcaatgtt gctatggttt ttaagtacgc taagacgtaa
     1081 acccagtcaa aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg
     1141 tctggcgcag tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt
     1201 taacgacggc tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg
     1261 cgaccgcttt gttgccgtca cgtaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg
     1321 cctcaattat gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc
     1381 cgatcaagac ggaatgggct atatgctggc agaaattaag cgcggtgctg atgccgtagg
     1441 cggcattccc tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt
     1501 aaagttgccg atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt
     1561 tattatcagc ggtgcctgcg gaatgttccg tactgatgta ttgcgtaagt tcggtttctc
     1621 ggatcgtact aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg
     1681 tattcggcag gcgaatcgct gcatcgtata cccacaggaa tgcaacagtc cgcgtgagga
     1741 gtggcgtcgc tggcggcgtt ggattgtggg ctacgcggtc tgtatgcgcc tgcataaaag
     1801 gcttttattt agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgattta
     1861 tggcgttggg atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg
     1921 tgtggtgttg gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt
     1981 tagcgcctgg tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta
     2041 tgtattatta gcttatgcca tctggattat ctatggactt attgcctttt ttactggacg
     2101 cgaacctcag cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta
     2161 ttcccaacct tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa
     2221 attattcaga ttgcagacca ggtaattatt gatgaaactg caggtgaagt tgttatcggc
     2281 gcgaataccc gtatttgtca tggtgccgtt attcagggtc cggtagtgat tggcgcaaac
     2341 tgcctgatag gtaattatgc gtttattcgt cctggcacaa taatcagcaa tggtgtaaaa
     2401 attggttttg ccaccgaaat taaaaatgcg gttattgaag cggaagcaac gattggtccg
     2461 caatgttata ttgccgactc ggtagttgca aaccaggcat atttgggcgc acaagtacgt
     2521 accagtaatc atcgtctgga tgaacagcct gtgtctgttc gaactccaga gggaattatc
     2581 gctaccggat gcgataaatt aggttgttat atcgggcagc gttcacgcct tggcgtacaa
     2641 gttattattt tgcctgggcg aattatttcc ccgaacacgc aacttggccc gcgcgtgatt
     2701 gtagaacgta atttacctac cggaacttat tcactccgac aagaacttat ccgtacagga
     2761 gattaaaatg ataaaacgga cgttattagc ggcggccatt tttagcgcat tgcccgctta
     2821 tgccgggtta acttccatta ccgcgggcta cgattttacc gattattctg gcgatcatgg
     2881 caaccgtaat ttagcgtatg ctgaactggt ggcgaaagtt gaaaacgcaa cgctgctttt
     2941 taatctttcc caggggcgtc gtgattatga aactgaacat ttcaatgcca ctcgcggtca
     3001 gggtgcggtc tggtataaat ggaataactg gctgacaacc cgaacgggta ttgcctttgc
     3061 ggataatacg ccggtctttg cccgccagga ttttcgtcag gatattaacc tggccctgtt
     3121 gccaaaaacg cttttcacga ccggttatcg ctacactaaa tattacgatg atgtcgaagt
     3181 cgatgcctgg caaggcggcg tatcactcta tactggcccg gtgatcacca gctaccgcta
     3241 tacccattat gactccagcg atgcaggtgg tagttatagc aatatgattt ccgtgcgtct
     3301 gaatgacccg cgcggcactg gttatacgca actatggcta agccgcggaa caggcgctta
     3361 cacctatgac tggacgccag aaacacgcta cggcagcatg aagagcgtca gtttgcaacg
     3421 tattcaaccg ctgactgagc aacttaatct cgggctgacg gcaggtaaag tgtggtacga
     3481 caccccaacc gatgattata acggtctgca acttgcagcc catctgacct ggaaattctg
     3541 attgcttctg ccgcccgcta tccggggcgg ccttccctgc cgattagccc cactcccttt
     3601 cctcgttgtt ttccgaccac attcaccgga taaattttat tctccagcgt tatatactat
     3661 agggaggtat gcattgacat atagcatacc tccctatagt atattgcgtg cagatgatga
     3721 ggtgcgaaat gcccagtact ccggaagaga agaaaaaggt ccttactcga gttcgtcgta
     3781 ttcgggggca gattgatgct ctggaacggt cgctggaggg tgatgccgaa tgccgtgcca
     3841 tactccaaca gatcgctgcc gttcggggcg cggctaacgg gctgatggca gaagtgcttg
     3901 aaagccatat ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa gtcagccaat
     3961 ccgttgacga cactattgaa ctggttcgag cctatcttaa atagctgaat ctattaccat
     4021 attgaggaag agcgagagat gaaatcacgt gctgccgttg catttgctcc cggtaaaccg
     4081 ctggaaatcg ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc
     4141 acccataccg gcgtttgcca taccgacgca tttaccctct ccggggatga cccggaaggt
     4201 gtattcccgg tggttctcgg tcacgaaggg gccggcgttg tggttgaagt cggtgaaggc
     4261 gtaaccagcg tcaaacctgg cgaccatgtg atcccgcttt acaccgcgga gtgcggcgag
     4321 tgtgagttct gtcgttctgg caaaactaac ctctgtgttg cggttcgcga aacccagggt
     4381 aaaggcttga tgccagacgg caccacccgt ttttcttaca acgggcagcc gctttatcac
     4441 tacatggggt gctctacatt cagtgaatac accgtggtcg cggaagtgtc tctggccaaa
     4501 attaatccag aagcaaacca tgaacacgtc tgcctgctgg gctgtggcgt gaccaccggt
     4561 attggcgcgg tgcacaacac agctaaagtc cagccaggtg attctgttgc cgtgtttggt
     4621 cttggcgcga ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc gggacggatt
     4681 atcgctatcg ataccaaccc gaagaaattc gatctggcgc gtcgcttcgg tgctaccgac
     4741 tgcattaacc cgaatgacta cgacaaaccg attaaagatg tcctgctgga tatcaacaaa
     4801 tggggtatcg accatacctt tgaatgcatc ggtaacgtca acgtgatgcg tgcggcgctg
     4861 gaaagtgcgc accgcggctg gggtcagtcg gtgatcatcg gggtggcagg ttctggtcag
     4921 gaaatctcca cccgtccatt ccagttggtc actggtcgcg tatggaaagg ttccgcgttt
     4981 ggcggcgtga aaggtcgttc ccagttaccg ggtatggttg aagatgcgat gaaaggtgat
     5041 atcgatctgg aaccgtttgt cacgcatacc atgagccttg atgaaattaa tgacgccttc
     5101 gacctgatgc atgaaggcaa atccattcga accgtaattc gttactgatt tcccgcaggt
     5161 ataccccgtc cacttcagac ggggttctta atactctccc cgggcagccg tccgggggat
     5221 taaccgtgag ataatgactg atggaactca ttgaaaaaca tgccagcttt ggcggctggc
     5281 aaaatgtgta tcggcattat tcccaatcac tgaaatgtga aatgaatgtc ggcgtctatc
     5341 tcccaccaaa agccgcgaat gaaaaattgc cggtgctgta ctggctttca ggcctgacct
     5401 gcaacgagca gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gagcacaaca
     5461 ttattgttgt tgcgccggac accagtccgc gaggcagtca tgtcgcagat gccgaccgtt
     5521 acgatctcgg gcaaggtgcc gggttttacc tgaacgcaac gcaagcgccg tggaatgaac
     5581 attacaaaat gtatgactat atccgcaacg agctgccgga tttagtgatg caacattttc
     5641 cggcaacgac cagaaagtct atctctggtc attctatggg cgggctgggc gcgctggtgc
     5701 tggcgttacg taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc
     5761 catcgcaagt accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagatg
     5821 cctggttgga ttacgatccg gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca
     5881 tggttgatca ggggttgagt gatgattttt acgcggaaca gctgcggact ccaaatcttg
     5941 aaaagatctg ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttacgacc
     6001 acagctatta ttttgtctcc agttttattg gcgagcatat tgcctaccac gccaataaac
     6061 tgaatatgcg ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc ttatccggcc
     6121 tacacttcgc ccgtaaaccg taggcctgat aagacgcgca aagcgtcgca tcaggcatgg
     6181 cgcacgactg ccggatgcgg cgtgaacgcc ttatccggtc tacacttcgc ccataaaccg
     6241 taggcctgat aagacgcgca tagcgtcgca tcaggcatct gcgcacgact tatctatgtt
     6301 taccacatca aatcatcagg cactttgaag tcggcatacg gatcgtcttc atcctgctct
     6361 tcggcactga gcgcgctgtg caacacaata ctgctggcat cacgctgagc aattttatcg
     6421 gcaacactgg cgggaataat ggtgtattca ccttcgctat tgttatcgac caacaagcgg
     6481 gcaatcgcca gacgaccatt aatcaactgc gcctgtgtga gcttatcgac aaaaatcttc
     6541 ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc cattggcaat ggtgattcgg
     6601 ttcatttcaa tgagctgctt cacctgggct ttatattctt tcgccaacgc cgcttgtttt
     6661 tgctgttcgc taagctgttt atcacgctca agctgtgcct ttttattttc ttctaccgca
     6721 gcccgagctt cacgcgcctg aacgcgcgat tttttcgccg ttctctcgac cttcgccgct
     6781 tttttgctgg tgactaatcc tgctttgagc aactgctctt gtaaggtaag ttttgccatc
     6841 tttgtttcca aacccgctga ataattttta gcgagtatac ctgtaaacgc cgtgggtgta
     6901 ttgggggcaa gtccctttcg cccctgctta ataaggcgcg tcaggcatcg gcacacggct
     6961 gcattcttga tctccgactc atcaaaataa acaccgccaa cccagcaaca agaatacccg
     7021 gtgcagaagc ggccattacg ccgaccgtgc cagtgcctaa tgccagcatt ttcccggcca
     7081 gtaacggacc gctcatagcc cccagacgcc ctaccgccac ggctgttccc acacctgttg
     7141 cgcggatctg cgaactgtaa aacaacagtg ccagggcata caaaacgctt tgcccacctg
     7201 tcgcaaacaa ccccgcgaca aatcccgcca gcaacatacc gttaaatgac gacaccgttc
     7261 caagcgccag cagcgaagct aacatgccgc tataaatcag tagcgacatg gttactggac
     7321 gcagcttatc catcaatgcg cccaacatta acgtcccgct tgccgccccc atttgcaggg
     7381 caaacatcac ccctgccgcc tgcgatggct ggaatccttg ctccaccaaa agtagcggta
     7441 gccagttgat caacatataa accaccagca gagtgaagaa ataacacaac cacagcagca
     7501 gcgtcgcggt tgccgtttct ggcgcaaata aggcacgcag tggtggcgca gcctgttttt
     7561 cgccagcgaa aaccgctgac tccggcagcc agcgcattaa tagcggcacc agaatcaacg
     7621 gcaccacacc acctacccaa aacaccgttt gccatgctaa gtttgccccc gcgaaaccca
     7681 gtgtcgccgc cagcgccgcg ccaatgggaa caccgcaata catcaggctc actgccgtcc
     7741 cacgaaaacg tggacccgcg gcttcagatg tcagggcgat aagattcggc aacgccgccc
     7801 ccagcccgac accggtcatc agccgcgcaa agaccagtga ggggaaatcc caggcaatcg
     7861 ccgttgccag tgagaacaaa ccaaacagcg caactgagcc aatcaaaata cgcttacgac
     7921 cataacggtc tgccagcatc ccgccaacca acgcgccggg tagcaaaccg agtattccgg
     7981 cgctaaatat ccagcccatt tgcattttat cgagtgcgaa agcctgggcg atgccacccg
     8041 ccgcaatgcc agccgcctga agatccagcc cttccatcag agcgaccaaa aaacaaagcc
     8101 cgatggtcag catcaggcgg gatgaagatg atgaaggggt acgagtcgac ataagatgaa
     8161 cctcattgcg agtgcaggtg aatcgcgact gatacattgc cagccgcgta atcagaacta
     8221 tttaatgact gcctacgggc gaaatgtagc gaaatgtagg ccggataagg cgttcacgcc
     8281 gcatccggca gtcgtgcgca gatgcctgat gcgacgctaa cgcgtcttat cagacctaca
     8341 aattctcacg ccatccgtag gccggacaag gcgttcacgc cgcatccggc tgtcgtgctg
     8401 ggatgggcat acaaaatgca cgttatcggc agtcatcatt atttgttgtt gcgcagatcc
     8461 agcgccacgt cgacgatcat atcctcctgg ccgccaacca tccggcgttt gcccagctca
     8521 acgagaatat ccaccgcact taagccataa cgcgccgccg ccgtttcaca gtgacgcagg
     8581 aagctcgagt aaacaccagc gtatcccagc gccagcgttt cgcgatcgac tcgtaccgga
     8641 cgatcctgca acggacgcac caggtcgtcg gcggcatcca ttaacgcata gagatcggtc
     8701 ccatgctgcc agcccagttt atccgcggcg gcaataaaca cttccagcgg tgcgttacct
     8761 gcgcccgcgc ccattcccgc gaggctggcg tcgattcggt cgcagccctc ttccaccgcc
     8821 gcgatagagt tcgccacgcc aagactcagg ttatggtgag cgtgcatgcc ggtttgcgtt
     8881 tctggtttca acactgcttt cagggcgcgg aaacggtcac ggatatcgct catgttcatc
     8941 gcaccgccag aatccaccac ataaatacag gtcgcaccgt agccttccat cagctttgcc
     9001 tgcttggcga gattctccgg cgtggtcata tggctcatca tcagaaaacc aacggtgtcc
     9061 attccgagct cgcgggcata ctgaatatgc tgggcggaaa catcagcttc ggtacagtgc
     9121 gttgccacac gaaccacccg cgcgccagcc tgccaggcat ttttcagatc gtgaatagtg
     9181 ccgattcctg gcagcaacaa cgtcgcgatt ttggcgtgcc tcaccacatc cgccgccgct
     9241 tcaatccatt caaggtcgct atgtgcgccg aaaccatagt taaagctgga accttgcaaa
     9301 ccgtcgccgt gggccacttc aatcgaatcc acgcgggcat cgtccagtgc tttggcaatc
     9361 tggcgaacgt tttccagcga atactgatga cgaatggcgt gcataccgtc acgcaatgtg
     9421 acgtccgaga tataaagttt tttaccgttc atgccgcttc tcctgccttg cgcgccagtg
     9481 actgggccat tttttccgct gtcgccagcg cactggaagt cataatatcg aggttgcccg
     9541 cataggcagg cagataatgc gctgcgcctt cgacttccag ccagaccgct gttttcagtc
     9601 cggagaattg ccccacgccc ggtaaattga ccggtttatc ctgcgggata acttcaaact
     9661 gcacgcgctg tttcaggcga taacccggta cgtaagcctg caccgcctca gccatttcat
     9721 tgattgaggc ttcgatatca tcttgtgaag cttcgtcgct caatacatac accgtgtcac
     9781 gcatcatcaa cggtggctct gctgggttaa gcacaataat cgccttccct tttgccgcgc
     9841 cgcccaccac ttcaatggct cgggaagtgg tttccgtaaa ttcatcgata ttggcacgcg
     9901 tgccaggtcc ggcagattta ctggcgatag aagcgataat ttcggcgtaa tgaacacgcg
     9961 ccacgcgtga aactgccgcc accattggaa tggtggcctg gccgccgcag gtgaccatgt
    10021 tgacgttcag ttgatcgacg ttcgcctcga ggttaaccac cggcacgcag taagggccga
    10081 tggcagcagg cgtcaggtca attaagcgaa tatccggttt cgcttcgcgt aaagcggcat
    10141 cgtttttcac atgcgcgccc gcgctggtcg catcaaatac aatgtcgata tcagcaaatt
    10201 caggcatgtt catcagcccg atcacccctt catgggtggt ggcgacgccc atacgtctgg
    10261 cacgcgccag accgtcggac tgaggatcaa tgccgaccat caccgccatc tccagatgct
    10321 gaccgtgacg caaaatttta atcatcagat cggtgccaat gttgccagaa ccgataatgg
    10381 cgactttacg cttactcatg acagacttcc ttttggggct gcgcttgaaa atgtcgcagc
    10441 aactgaacct atgccttcaa tatgggcttc aaaacgatcg cccgcattca ccgccaccat
    10501 cgggcctaat gccccggtaa gaatgatatc tccggcgcgc agcggttcac ccagactggc
    10561 cattttgcgt gccagccaga cggcggcatt aagcggatgt cccaggcatt cgctgccgcg
    10621 cccgctggaa acctcttcgt tattacgcgt catcttcatg gcgcagtttt tcaggtctaa
    10681 ccccgccgga cgttgcgccg gaccgccgat gacatacacc ccgcaggaag cgttatctgc
    10741 cacggtatcg acaaactgaa tcgaccagtc gcgaatgcgg ctccccacca cttccagcgc
    10801 cggaagtacc cattcaatgg cgttatacag ttcgtcgaag gtgatatcag ttgcgggtaa
    10861 atcgcggttc aacaccagtg cgatctccgc ttcaatgcgg ggttgcagaa cacgggaaaa
    10921 aggaatgatt tcgttatcgc cataacacat gtcggcaaat aacgtcccaa aatccggttg
    10981 atcaacgccc agttgttgtt gcactttcgg atgcgtcagg cccactttac gccctaccac
    11041 gcgacgcccc tgcacaac
//
LOCUS       DAJVXP010000128        11000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVXP010000128 DAJVXP010000000
VERSION     DAJVXP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.128"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..277)
                     /locus_tag="OQS10_003277"
     CDS             complement(<1..277)
                     /locus_tag="OQS10_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2294229.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="OQS10_003278"
     CDS             complement(444..1184)
                     /locus_tag="OQS10_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS2294230.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OQS10_003279"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OQS10_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS2294231.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OQS10_003280"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OQS10_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS2294232.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OQS10_003281"
     CDS             complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OQS10_003281"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS2294233.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OQS10_003282"
     CDS             complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OQS10_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS2294234.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OQS10_003283"
     CDS             complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OQS10_003283"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS2294235.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7167..7598
                     /locus_tag="OQS10_003284"
     CDS             7167..7598
                     /locus_tag="OQS10_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2294236.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7621..8199
                     /locus_tag="OQS10_003285"
     CDS             7621..8199
                     /locus_tag="OQS10_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS2294237.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8200..8907
                     /locus_tag="OQS10_003286"
     CDS             8200..8907
                     /locus_tag="OQS10_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS2294238.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8895..9572
                     /locus_tag="OQS10_003287"
     CDS             8895..9572
                     /locus_tag="OQS10_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS2294239.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9566..10243
                     /locus_tag="OQS10_003288"
     CDS             9566..10243
                     /locus_tag="OQS10_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS2294240.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10215..10928)
                     /locus_tag="OQS10_003289"
     CDS             complement(10215..10928)
                     /locus_tag="OQS10_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS2294241.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10925..>11000)
                     /locus_tag="OQS10_003290"
     CDS             complement(10925..>11000)
                     /locus_tag="OQS10_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2294242.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2918 c         2740 g         2715 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagtgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3061 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3121 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3181 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3241 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3301 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3361 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat
     3421 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3481 tatcgatgtt catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3541 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg
     3601 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3661 aaacaccgtg tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca
     3721 gttcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3781 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3841 tgccgatttt ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3901 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt
     3961 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     4021 cagtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4081 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4141 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4201 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4261 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4321 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4381 catcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4441 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4501 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4561 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4621 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4681 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4741 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4801 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4861 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4921 gccgatgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4981 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     5041 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5101 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5161 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5221 gctgccaaca gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa
     5281 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5341 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5401 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5461 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5521 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt
     5581 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5641 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5701 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5761 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5821 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5881 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5941 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     6001 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6061 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6121 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6181 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6241 catggcggag tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa
     6301 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6361 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6421 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6481 ggagccatat acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag
     6541 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6601 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6661 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6721 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6781 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6841 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6901 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6961 aatgattgtc cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc
     7021 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7081 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7141 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg
     7201 cacgctttta tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7261 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7321 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag
     7381 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7441 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7501 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7561 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7621 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7681 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7741 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7801 accaatttac tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg
     7861 atggtcatcg gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca
     7921 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7981 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     8041 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg
     8101 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8161 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8221 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg
     8281 atctatagcg cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcaggca
     8341 aaatatgtcg cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8401 tggctaacag aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt
     8461 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8521 cccgtgcaat gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8581 ctgtttgccg gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8641 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8701 atgcccgcgt taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8761 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8821 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa
     8881 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8941 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     9001 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9061 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9121 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9181 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9241 aaacagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9301 gccatcgtca cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg
     9361 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc
     9421 aggccagcga aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga
     9481 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9541 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc
     9601 tagcgcagca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9661 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9721 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9781 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9841 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9901 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9961 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
    10021 actctctccg ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10081 gagcgtgttt gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat
    10141 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10201 tgtaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10261 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10321 taaaaggctt ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc
    10381 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10441 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10501 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10561 caatcccctt ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct
    10621 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10681 ccatccatcg ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc
    10741 cagaaggtgg ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10801 cgccgctgcg gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg
    10861 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10921 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10981 ccattccgct aaaacgcgcc
//
LOCUS       DAJVXP010000129        10875 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVXP010000129 DAJVXP010000000
VERSION     DAJVXP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.129"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..201)
                     /locus_tag="OQS10_003291"
     CDS             complement(<1..201)
                     /locus_tag="OQS10_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCS2294243.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFD"
     gene            complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OQS10_003292"
     CDS             complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OQS10_003292"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS2294244.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OQS10_003293"
     CDS             complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OQS10_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS2294245.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OQS10_003294"
     CDS             complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OQS10_003294"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS2294246.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OQS10_003295"
     CDS             complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OQS10_003295"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS2294247.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OQS10_003296"
     CDS             complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OQS10_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS2294248.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OQS10_003297"
     CDS             complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OQS10_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS2294249.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OQS10_003298"
     CDS             complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OQS10_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS2294250.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7370..9328)
                     /gene="cheA"
                     /locus_tag="OQS10_003299"
     CDS             complement(7370..9328)
                     /gene="cheA"
                     /locus_tag="OQS10_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS2294251.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     ILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWKVF
                     NVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATIL
                     GDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9333..10259)
                     /gene="motB"
                     /locus_tag="OQS10_003300"
     CDS             complement(9333..10259)
                     /gene="motB"
                     /locus_tag="OQS10_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS2294252.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10256..>10875)
                     /gene="motA"
                     /locus_tag="OQS10_003301"
     CDS             complement(10256..>10875)
                     /gene="motA"
                     /locus_tag="OQS10_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS2294253.1"
                     /translation="QMGMFSLERDIENPRESEIFASYPRILADSVMLDFIVDYLRLII
                     SGHMNTFEIEALMDEEIETHESEAEVPANSLALVGDSLPAFGIVAAVMGGVHALGSAD
                     RPAAELGALIAHAMVGTFLGILLAYGFISPLASVLRQKSAETSKMMQCVKVTLLSNLN
                     GYAPPIAVEFGRKTLYSSERPSFIELEEHVRAVKNPQQQTTTEEA"
BASE COUNT         2440 a         3083 c         2666 g         2686 t
ORIGIN      
        1 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
       61 aaaccagata acacacacgc ccactaacaa aatcagcaac gaggtcagtt cacgggaacg
      121 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      181 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagct taaatgatgc
      241 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
      301 tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga
      361 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
      421 caacaaatcg tccacctgat cctgactggc taccacgccg actttgctgg tatcgacctg
      481 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
      541 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
      601 aatgaccagc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
      661 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag
      721 ttcacgggca tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
      781 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
      841 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
      901 ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
      961 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     1021 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     1081 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     1141 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     1201 ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc aggccatcca
     1261 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     1321 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     1381 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     1441 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     1501 catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     1561 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     1621 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     1681 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     1741 ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg cgccactacg
     1801 cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca agacacgttc
     1861 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     1921 gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg aaagcggcaa
     1981 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     2041 tttttcagaa ctcaacaacg gacccgcctt cagcgttgtc ggtgctgaca atggcttatg
     2101 tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt cgctatacgc
     2161 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     2221 cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca tcacaacggg
     2281 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     2341 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
     2401 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     2461 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     2521 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     2581 ttttcagagt aacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
     2641 aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     2701 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc aaaatcaaca
     2761 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     2821 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     2881 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
     2941 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     3001 gaagccgccg cgctccatac gcgatactcg ccagaacggc gatgtgcgtg atccgcgagc
     3061 agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
     3121 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
     3181 tccatcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
     3241 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
     3301 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
     3361 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
     3421 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
     3481 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
     3541 gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac ctgctcaatc
     3601 ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt cacgcgggtc
     3661 accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
     3721 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta
     3781 cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc ctgctctccg
     3841 gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
     3901 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
     3961 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc
     4021 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
     4081 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
     4141 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
     4201 tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa aatggcggtg
     4261 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
     4321 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
     4381 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagtgt cgaaatctga
     4441 tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc cagctgccag
     4501 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
     4561 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
     4621 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
     4681 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac
     4741 gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt gttcagcgcg
     4801 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
     4861 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
     4921 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
     4981 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
     5041 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
     5101 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
     5161 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agcggcggcg
     5221 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
     5281 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc aatctcgccc
     5341 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccggcgctt
     5401 tcgaccagca ccgaaccggt atcaacgcgt gcgacggagt cttcaatgag ggctttgatc
     5461 tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca
     5521 aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata
     5581 ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta
     5641 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
     5701 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
     5761 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
     5821 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
     5881 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
     5941 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgctagg
     6001 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt aagcaacata
     6061 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
     6121 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
     6181 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
     6241 gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat cagttcagtt
     6301 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
     6361 ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc
     6421 ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc
     6481 atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
     6541 gtcagctcgc cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg
     6601 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
     6661 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
     6721 aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg
     6781 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
     6841 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
     6901 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
     6961 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
     7021 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
     7081 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
     7141 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
     7201 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
     7261 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
     7321 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
     7381 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
     7441 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
     7501 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
     7561 agcggcgacc gccactttgt aagatcacaa caattccctg ggtggcttcg gttttcgcgc
     7621 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
     7681 gtacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc agtgattcca
     7741 taacagcatt cagcggcaga atgaaaactt catccccaac gcgtacggac atgccgtcga
     7801 ggatggtcag cggcagtaaa atgcggatcg tagtgccagt cccctgcttc gactggattt
     7861 cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc
     7921 cggagacgtc ggtgacctgc tctgccatgg agaagcctgg tgcaaatatc agcatcgcga
     7981 cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa
     8041 ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct
     8101 gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt
     8161 ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa
     8221 tgcgttctat caggcttttg tcgagttcag tagaactgcc caccagcgtc agttctacct
     8281 gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta aaaacatatt
     8341 ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta
     8401 actgccccat gctggttatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct
     8461 gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct
     8521 tttctaccgc tacacggatg ctggtggatt cattgctgcg cgtcgttttt tcccgctcca
     8581 cgcggccggt tggcgcttgt tcggctgcca gtttaagcac tggtggggtg gatatttttg
     8641 gcgagacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg
     8701 ctgtgatgtc atcttcggcg atgtcgcccg gtaatattgc cgagagcgaa tccgcccctt
     8761 tcaccacgtc agttaacgtt gtcagatgtc ccagttcttc ttccagcagg tcgacttccc
     8821 cggccttcag gcgcgaaagg ataattcgtc gcggcgactg actgcgactc tgctcatctt
     8881 gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg
     8941 cttctaatgc cagttgacgc aaggcctggc agatataatc gaagctggcg gcatccggct
     9001 cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca
     9061 gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt
     9121 ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga
     9181 tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca
     9241 aaaccagcag atgctgctcc atgtcagcca acagttcgtc cgcttcatca aaaaatgtct
     9301 gataaaaatc gcttatatcc atgctcacgc tgtcacctcg gttcggctga tggcattgtg
     9361 ggaacactga cctgtggtgc aacctcaggt ttttccaggg cgcttactgg ctcattctgg
     9421 ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac cagcaggctg
     9481 atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg
     9541 ccgacgacac gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat
     9601 gcattggccc gatcggcaga aagctcccag ttgctatatc ctttctcacc gctggcgtag
     9661 gggaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagtacaggc
     9721 gcaatgacac gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac
     9781 atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt
     9841 ttgagatggg gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca
     9901 ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcagatcttc gatgttcggc
     9961 tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt
    10021 tcactattag aaatgcgatc gccgcccgta accgcagtcg ccagtggagt ccggaagtac
    10081 tccgcaatct gaatcagctc ttttgggctg gagatggaga tcagccacat caccagaaaa
    10141 aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc
    10201 ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg
    10261 cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt
    10321 caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag
    10381 gcggtgcgta accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc
    10441 tggtttcggc gcttttctga cgtaaaacac tcgctaatgg ggaaataaat ccgtaagcca
    10501 ataaaatgcc gaggaaagtc cccaccatcg catgtgcgat aagcgcaccc agctcggcgg
    10561 caggacgatc ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa
    10621 acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt
    10681 cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc atatgaccgc
    10741 tgataatcag acgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc
    10801 gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa
    10861 acatccccat ctgcc
//
LOCUS       DAJVXP010000130        10866 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVXP010000130 DAJVXP010000000
VERSION     DAJVXP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.130"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..147)
                     /locus_tag="OQS10_003302"
     CDS             complement(<1..147)
                     /locus_tag="OQS10_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS2294254.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="OQS10_003303"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="OQS10_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="OQS10_003304"
                     /pseudo
     CDS             476..1021
                     /locus_tag="OQS10_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OQS10_003305"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OQS10_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS2294255.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="OQS10_003306"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="OQS10_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS2294256.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="OQS10_003307"
     CDS             3028..3201
                     /locus_tag="OQS10_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294257.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OQS10_003308"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OQS10_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS2294258.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="OQS10_003309"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="OQS10_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS2294259.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="OQS10_003310"
     CDS             complement(5499..6422)
                     /locus_tag="OQS10_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2294260.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="OQS10_003311"
     CDS             6639..7982
                     /locus_tag="OQS10_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS2294261.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="OQS10_003312"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="OQS10_003312"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS2294262.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="OQS10_003313"
     CDS             10002..10226
                     /locus_tag="OQS10_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS2294263.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="OQS10_003314"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="OQS10_003314"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS2294264.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
BASE COUNT         2816 a         2511 c         2785 g         2754 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgt
//
LOCUS       DAJVXP010000131        10823 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVXP010000131 DAJVXP010000000
VERSION     DAJVXP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.131"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            528..2456
                     /gene="thrS"
                     /locus_tag="OQS10_003315"
     CDS             528..2456
                     /gene="thrS"
                     /locus_tag="OQS10_003315"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS2294265.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2460..3002
                     /gene="infC"
                     /locus_tag="OQS10_003316"
     CDS             2460..3002
                     /gene="infC"
                     /locus_tag="OQS10_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS2294266.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3099..3296
                     /gene="rpmI"
                     /locus_tag="OQS10_003317"
     CDS             3099..3296
                     /gene="rpmI"
                     /locus_tag="OQS10_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS2294267.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3349..3705
                     /gene="rplT"
                     /locus_tag="OQS10_003318"
     CDS             3349..3705
                     /gene="rplT"
                     /locus_tag="OQS10_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS2294268.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3822..3946
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3828..3872
                     /gene="pheM"
                     /locus_tag="OQS10_003319"
     CDS             3828..3872
                     /gene="pheM"
                     /locus_tag="OQS10_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS2294269.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4155..5138
                     /gene="pheS"
                     /locus_tag="OQS10_003320"
     CDS             4155..5138
                     /gene="pheS"
                     /locus_tag="OQS10_003320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS2294270.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5153..7540
                     /gene="pheT"
                     /locus_tag="OQS10_003321"
     CDS             5153..7540
                     /gene="pheT"
                     /locus_tag="OQS10_003321"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS2294271.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7545..7844
                     /gene="ihfA"
                     /locus_tag="OQS10_003322"
     CDS             7545..7844
                     /gene="ihfA"
                     /locus_tag="OQS10_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS2294272.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7945..8925
                     /gene="btuC"
                     /locus_tag="OQS10_003323"
     CDS             7945..8925
                     /gene="btuC"
                     /locus_tag="OQS10_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS2294273.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8988..9539
                     /gene="btuE"
                     /locus_tag="OQS10_003324"
     CDS             8988..9539
                     /gene="btuE"
                     /locus_tag="OQS10_003324"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS2294274.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            9539..10288
                     /gene="btuD"
                     /locus_tag="OQS10_003325"
     CDS             9539..10288
                     /gene="btuD"
                     /locus_tag="OQS10_003325"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS2294275.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10366..>10823
                     /locus_tag="OQS10_003326"
     CDS             10366..>10823
                     /locus_tag="OQS10_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS2294276.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2714 a         2561 c         2927 g         2621 t
ORIGIN      
        1 cataatctat tcgccttata tatgtctgga ggttttgagg atgatgatta attattatcg
       61 ccagaacgaa atgtgcacga aaaattgcta taagaattgt gtcactttta tcattgtgat
      121 atgaccactt cgtcattaat ctcatgagcg tttaaaatac taccgccaaa tcacgcaacg
      181 aactttgctg gattcagttt atgctgctgt aaatccgctc gcagtaaacc tttcagacgc
      241 accgtgatgt tatcagtggt tctttaagcg ttttgctggt gtactcacta caaacgaatt
      301 gcgaatcgaa tcaatgtgaa acggaaaggt acaatctccc tttttttagt gttaacatcg
      361 ttcaaccggg tggaaggtga gaggttaatg tctttcattt gtgtacctga ttgcgaacca
      421 atttagcatt tgttggctag atggtttcgc aatgaactgt taataaacaa atttttcttt
      481 gtatgtgatc tttcgtgtgg gtcaccactg caaattaagg atataaaatg cctgttataa
      541 ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc
      601 tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg
      661 ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc gccaaagacg
      721 aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat gcgattaaac
      781 aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg
      841 acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc
      901 atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgaagcgc
      961 gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg
     1021 cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc
     1081 cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt
     1141 actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag
     1201 acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc
     1261 gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat
     1321 tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt cgttctaaac
     1381 tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg
     1441 aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct tctgagaacc
     1501 gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc
     1561 tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaacg
     1621 agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag gatgacgcgc
     1681 atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct
     1741 atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg
     1801 aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc
     1861 tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga
     1921 aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc
     1981 tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac aacgagcgta
     2041 aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc atcggtatcc
     2101 tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag gttgttatca
     2161 tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg
     2221 cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc
     2281 acactttgcg tcgcgtccca tatatgttgg tctgtggtga taaagaggtg gaatcaggca
     2341 aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga
     2401 tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta
     2461 ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
     2521 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
     2581 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
     2641 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
     2701 ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg
     2761 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
     2821 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
     2881 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
     2941 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt
     3001 aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt
     3061 gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac
     3121 gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta
     3181 acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag
     3241 ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg
     3301 ttaacgtttt taacttttta attagaatat agatacagga gagcacatat ggctcgcgta
     3361 aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac
     3421 tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag
     3481 tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcgcgt
     3541 atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa
     3601 aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta
     3661 gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag ttgaaagagg
     3721 gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc
     3781 cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta
     3841 ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga
     3901 aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa
     3961 aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac ggccagcgcg
     4021 gagaaaccgg agcgtattta agtacgtgag aatttcgagc actgcccggg accaaaatgg
     4081 caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc accactgaca
     4141 caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag gcggccatta
     4201 gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg ggtaaaaaag
     4261 ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag cgtccggcag
     4321 ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat gcgcgtaaag
     4381 cggaactgga aagcgcagcg ctgaatgcgc gtctggcggc ggaaacgatt gatgtctccc
     4441 tgctaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc atcgaccgta
     4501 tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa atcgaagacg
     4561 attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc gctgaccacg
     4621 acactttctg gtttgacact acccgcctgc tgcgtaccca gacctctggc gtacagatcc
     4681 gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgc gtttatcgta
     4741 acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg attgttgata
     4801 ccaacatcag ctttaccaac ctgaaaggca ctctgcacga tttcctgcgt aacttctttg
     4861 aggaagattt gcagattcgc ttccgtcctt cctacttccc gttcaccgag ccttctgcag
     4921 aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggttgc gggatggtgc
     4981 atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt ttcgccttcg
     5041 ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg cgttcattct
     5101 tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga ttatgaaatt
     5161 cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgatg cgctggcgaa
     5221 tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg gtagcttcca
     5281 cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg acaaactgcg
     5341 tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgtg gtgcgccaaa
     5401 ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc cgggtgattt
     5461 caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt gctccttctc
     5521 tgaactggga atttctgacg atcacagcgg cattatcgaa ctgcctgcag atgcgccgat
     5581 tggcactgac atccgcgaat acctgaaact cgatgacaac accatcgaaa tcagcgtaac
     5641 gccaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg ccgtgctgaa
     5701 tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg acgacacgtt
     5761 gccgattaca gtcgaagcgc ctgaagcctg cccgcgctat cttggccgtg tggtaaaagg
     5821 cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc gttgcgggat
     5881 ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac tgggccagcc
     5941 gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga tggcaaaaga
     6001 gggcgaaacg ctggtactgc ttgatggcac tgaagcgaag ctgaatgctg acactctggt
     6061 catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg aacactctgg
     6121 cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc cgctgtctat
     6181 caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt atgagcgtgg
     6241 cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg acccgtctgc tgatcgacat
     6301 ctgcggtggt gaggctggtc cggtaattga tatcaccaac gaagcaacgc tgccgaagcg
     6361 tgcaaccatc actttacgtc gtagcaaact ggatcgcctg atcggccatc atattgctga
     6421 tgaacaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag gcaaagacga
     6481 gtggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag atctggttga
     6541 agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac aggcaagcct
     6601 gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa cgctgctcaa
     6661 cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag tgcagcagat
     6721 gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg aaatgtcagc
     6781 aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc agaaccgtca
     6841 gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata ctcaggcacc
     6901 gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc gttacgaaga
     6961 gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg atcttgaatc
     7021 cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag cgaatccggc
     7081 actgcatccg gggcaatccg cagcgattta tctgaaaggg gaacgtattg gttttgttgg
     7141 agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc tggtgttcga
     7201 actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga tttctcgctt
     7261 cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg cagcggatat
     7321 tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact tatttgacgt
     7381 gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc tgatcctgca
     7441 agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca aatgtgtaga
     7501 ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg cttacaaaag
     7561 ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat gccaaagaac
     7621 tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa caggtgaaac
     7681 tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga cgtaacccga
     7741 aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga cccgggcaga
     7801 agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat ctaactaaaa
     7861 aggccgctct gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa atcaacgcca
     7921 tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca gcgacaaaat
     7981 attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt aagcctttgc
     8041 gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga actgttcgtc
     8101 tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc gctggctata
     8161 tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg actacttggc
     8221 gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca agggcaactc
     8281 cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac tttaatactc
     8341 ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg cgttgcatta
     8401 gggattatct gtagcgcact aatgacgtgg gctatctact tttccacctc tgttgattta
     8461 cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg gcaaagctgg
     8521 ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag gccgatgaat
     8581 atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg gttctggcgc
     8641 aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct ggcgggtgct
     8701 atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt aaccgatcat
     8761 cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cagtgctgct ggccgatatt
     8821 gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac cgcaacgttg
     8881 ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca aaaagacggt
     8941 ctatgattaa aagctaagat ttttactgac cacacccagg agaaacgatg caagattcca
     9001 ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag aagtacgccg
     9061 gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg caatatgagc
     9121 agttggagaa tattcagaaa gcctgggccg atcgaggttt tgtggtgctg ggattcccgt
     9181 gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact tactgtacca
     9241 ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaacggc gaaggacgcc
     9301 atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg gaagagagcg
     9361 gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct gtaccctgat gatattttat
     9421 ggaattttga gaaattttta gttggcaggg acggattggt catccagcgt ttttccccgg
     9481 atatgacgcc ggaaaacccc attgtgatgg aaagcattaa actggcgttg gcaaaataat
     9541 gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctagggc cactttctgg
     9601 cgaggttcgg gctggggaga tcctgcacct ggtggggccg aatggcgcgg gtaagagtac
     9661 cttactggca cgaatggccg gaatgaccag cggtaaggga agtattcagt tcgcggggca
     9721 accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc tttcacaaca
     9781 gcagacgccg ccgtttgcaa tgccggtctg gcactatctg acactgcatc agcacgataa
     9841 aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg acaaactcgg
     9901 acgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg ctgcggtggt
     9961 gctgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg atgagccgat
    10021 gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg ctctgtgtca
    10081 gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacattgc gtcatgcgca
    10141 tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag aagaggtgct
    10201 cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg atatcgaagg
    10261 tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc gggtaattac
    10321 gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg tttctgcctt
    10381 attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc gccgccaaat
    10441 gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct acaaagctgg
    10501 catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg ttcgggattt
    10561 gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc cccgagaaac caaagaacaa
    10621 gcctctatcg gcacgcaaat tgataaagac gagttgctgc ctggtgacct ggtctttttc
    10681 aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa caaccaattt
    10741 atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt ctattggcag
    10801 aaaaatttct ggcaggcgag acg
//
LOCUS       DAJVXP010000132        10814 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVXP010000132 DAJVXP010000000
VERSION     DAJVXP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.132"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..309
                     /locus_tag="OQS10_003327"
     CDS             <1..309
                     /locus_tag="OQS10_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS2294277.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OQS10_003328"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OQS10_003328"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS2294278.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQS10_003329"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OQS10_003329"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS2294279.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OQS10_003330"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OQS10_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS2294280.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OQS10_003331"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OQS10_003331"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS2294281.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OQS10_003332"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OQS10_003332"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS2294282.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OQS10_003333"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OQS10_003333"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS2294283.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OQS10_003334"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OQS10_003334"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS2294284.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OQS10_003335"
     CDS             complement(8117..9763)
                     /locus_tag="OQS10_003335"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS2294285.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OQS10_003336"
     CDS             complement(9766..9816)
                     /locus_tag="OQS10_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294286.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OQS10_003337"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OQS10_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS2294287.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..10814
                     /locus_tag="OQS10_003338"
                     /pseudo
     CDS             10354..10814
                     /locus_tag="OQS10_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2445 a         2984 c         2828 g         2557 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gccccctgat cactgttgag gtacatatca gtaaaggtct
    10441 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    10501 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10561 tgctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10621 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    10681 actggctctt acaggcgctc tgcgtggcgt tcccggtgca atctccagtg caactgaagc
    10741 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10801 taacggtgaa ggat
//
LOCUS       DAJVXP010000133        10775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVXP010000133 DAJVXP010000000
VERSION     DAJVXP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.133"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..344
                     /locus_tag="OQS10_003339"
     CDS             <1..344
                     /locus_tag="OQS10_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2294288.1"
                     /translation="FRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKL
                     HWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNY
                     LASVLAGSGLS"
     gene            514..792
                     /locus_tag="OQS10_003340"
     CDS             514..792
                     /locus_tag="OQS10_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCS2294289.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            792..1169
                     /locus_tag="OQS10_003341"
     CDS             792..1169
                     /locus_tag="OQS10_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294290.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1638..2774
                     /locus_tag="OQS10_003342"
     CDS             1638..2774
                     /locus_tag="OQS10_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2294291.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(2816..3586)
                     /gene="yafV"
                     /locus_tag="OQS10_003343"
     CDS             complement(2816..3586)
                     /gene="yafV"
                     /locus_tag="OQS10_003343"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS2294292.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            3767..4213
                     /gene="ivy"
                     /locus_tag="OQS10_003344"
     CDS             3767..4213
                     /gene="ivy"
                     /locus_tag="OQS10_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS2294293.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(4256..6700)
                     /gene="fadE"
                     /locus_tag="OQS10_003345"
     CDS             complement(4256..6700)
                     /gene="fadE"
                     /locus_tag="OQS10_003345"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS2294294.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            6940..7518
                     /gene="lpcA"
                     /locus_tag="OQS10_003346"
     CDS             6940..7518
                     /gene="lpcA"
                     /locus_tag="OQS10_003346"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS2294295.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            7724..8491
                     /locus_tag="OQS10_003347"
     CDS             7724..8491
                     /locus_tag="OQS10_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS2294296.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8462..9202)
                     /locus_tag="OQS10_003348"
     CDS             complement(8462..9202)
                     /locus_tag="OQS10_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS2294297.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(9358..9636)
                     /gene="yafQ"
                     /locus_tag="OQS10_003349"
     CDS             complement(9358..9636)
                     /gene="yafQ"
                     /locus_tag="OQS10_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCS2294298.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(9639..9899)
                     /gene="dinJ"
                     /locus_tag="OQS10_003350"
     CDS             complement(9639..9899)
                     /gene="dinJ"
                     /locus_tag="OQS10_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCS2294299.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            10085..>10775
                     /locus_tag="OQS10_003351"
     CDS             10085..>10775
                     /locus_tag="OQS10_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS2294300.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETI"
BASE COUNT         2868 a         2583 c         2675 g         2649 t
ORIGIN      
        1 cctttcggtc aataatagca gaacaaaaga aagagccaga aatgacggtc agatattata
       61 tcagttctgc tgatttaacc gcagaaaagt tcgccacagc aatccgaaac cactggcacg
      121 tggagaataa gctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa
      181 gaagaggaaa cgccgcagaa ttattttcag ggatacggca catcgctatt aatattttaa
      241 cgaatgataa ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gccatggata
      301 gaaactatct cgcgtcagtc cttgcgggga gcgggctttc gtaatctttc cctagggagt
      361 cagccgaatg ttgggaagga tttgacggat gaagagaaga actgcttggc cggcggcagt
      421 acgggaagcc cgggaggatg gggaccagaa gatgagaata gcgcaaggga tcgtatgcag
      481 catgaacggt ataaagatga attgcgacag aaaatgggaa aacccgacgt tacagattca
      541 aatttgaaaa atattataga cgatctttat cggccaaatg caaaagtagg aagtggaagc
      601 acagcagatg ctgttcgtta tgaattggcc acaggtgaaa aagttggtgg tagaggacat
      661 gtattgaaag cacaggatta tacaagggct ttacaggatt ggttggataa gaatccaaca
      721 gcatctccag gggatcgagc agcagcagaa aacgttcttc aggatttaca gaatgctttg
      781 ggaggcaagt aatgaaccaa tatattgcta gttatataga aaaaatggca tcattttcca
      841 ctgttttgtc tgagacagtc tcattagcta atgaatactg gcatccagaa acgccaccgg
      901 tggttatgtt gttctctcag gttggaaaaa cgattgtaaa aaatttttat gaaatggggg
      961 atattgaaag agaaaaaata tttaaacatg tcgaagatgg tatggtctcg cttgatgatg
     1021 aattggccac agttgtagct acagggctta tagaggcaat agtcactgcg accgatagca
     1081 attctttact ttggcaagat ttaagtaaat ttctgggtga caaatcaaag tctcatgcat
     1141 tagtgtggaa aaattttggt caggagtgat attattcaag aagtattaag ctcgctattg
     1201 agatcttaat gcttcttgag attaaattaa agttaggacg aagtaattgc aggtggaaat
     1261 aaatccatag tgacgtgtgg caatggctat tagatgttat ggtgtgatgg gctttggtga
     1321 tttaagagct aagttagatt gccttatgaa aaagtatggt tatatattta atgatgtgta
     1381 tcatatattt aaataaaaaa cggttctctc ttgtcaagat agtaatagga tagactattg
     1441 tgttgataaa tataattaag agtctacttt gttgatttta gaagactttg gagcagggaa
     1501 agatcatcgg aagtacactt tttgtactaa ataattcgcg ctaaatgttc aataattgag
     1561 atattcctta ttacctaaaa ctatttttca tagcttatat atgatcaaat actccttgca
     1621 taaataagga gaacaaaatg gaacttaaaa aactgatgga acatatttct attatccccg
     1681 attacagaca agcctggaaa gtagaacata aattgtcaga tattctactg ttgactattt
     1741 gtgccgtcat ttctggtgca gagggttggg aagatataga ggattttggt gaaacacatc
     1801 tcgatttttt gaagcaatat ggtgattttg aaaatggtat tcctgttcac gatactattg
     1861 ccagagttgt atcctgtatc agtcctgcaa aatttcacga gtgctttatt aactggatgc
     1921 gtgactgcca ttcttcagat gataaagacg tcattgcaat tgatggaaaa acgctccggc
     1981 actcttatga caagagtcgc cgcaagggag cgattcatgt cattagtgcg ttctcaacaa
     2041 tgcacagtct ggttctcggg cagattaaga cggatgagaa atccaatgag attacagcca
     2101 ttcctgaact tcttaacatg ctggatatta aaggaaaaat catcacaact gatgcgatgg
     2161 gatgccagaa agatattgca gagaagatac aaaaacaggg cggtgattat ttattcgcgg
     2221 taaaaggaaa ccaggggcgg cttaataaag cctttgagga aaaatttccg ctgaaagaat
     2281 taaataatcc agagcatgac agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa
     2341 tccgtcttca tattgtttgc gatgtccctg atgaacttat tgatttcacg tttgaatgga
     2401 aagggctgaa gaaattatgc gtggcagtct cctttcggtc cataatagca gaacaaaaga
     2461 aagagccaga aatgacggtc agatattata tcagttctgc tgatttaacc gcagagaagt
     2521 tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg cgtctggacg
     2581 tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa tgcagcagaa ttattttcag
     2641 ggatacggca catcgctatt aatattttga cgaatgataa ggtattcaag gcagggttaa
     2701 gacgtaagat gcgaaaagca gctatggaca gaaactatct ggcgtcagtc cttgcggaga
     2761 gcgggctttc gtagtcttac cccgactctc ccccagcctt aaacacaacc cccactcacc
     2821 gcaacctaaa ctcatccgca tcctgccatg ccggaaactt ttctctatat tcccgcaatg
     2881 ccatcatcga cagctccgca tcaatgcgcg ttgcctgatg cgcgtcggca gtagcgataa
     2941 tctcgccttg tggattaatc acccggctgt caccgcgata atggcagccg ttgccatcgc
     3001 tgccgacgcg attgcatccc gccacatacg cctgattctc aatcgcgcgg gccgtcagca
     3061 atgcctgcca gtgcagagag cgcggagcag gccagttggc aacgtagatg gcgaggtcat
     3121 aatcgttgag attgcgcgac cacaccggaa aacgtaagtc gtagcacacc aacggcaaaa
     3181 tacgccagcc gcgccattcc acaatcactc gcgcattgcc cgctttataa tgtagatgct
     3241 catctgccat gcggaacaga tgacgcttat cataaaaatg taccgtgccg cccggctcaa
     3301 ccagcaaaaa gcggttaacc gaaccagact ccgtttgtaa tgcaacactg cctgcaatca
     3361 gcgcattgca ctgctgcgcc ttagctgtca tccagtccac tacgtcatct tgtgctagcg
     3421 acgaagctgc cgcttccatg gcaaagccgc tggtaaacat cttcggtaga acgatcacat
     3481 cgcgcccggt aataccttcc agttgacgat caaaatgacg caggttggca gggccatcca
     3541 tccacaccag tggttgctgc aaaagcgtaa tcttcaaacc aggcacggtg tacaactcct
     3601 ttatgcgaag ggttttataa ctttaacacc ttatcaggca gttgccttag cgcagaataa
     3661 attgataaca aatgctgata ttggaaatat ctgatttgca aattatcgtg ttatcgccag
     3721 gctttagaag gttaataaca tgggcaggat aagctcggga ggaatgatgt ttaagacaat
     3781 aacgacagtc gccgctctgg tcatcgccac cagtgcaatg gcgcaggatg atttaaccat
     3841 tagcagcctt gcaaagggcg aaaccaccaa agctgcattt aatcagatgg tacaagggca
     3901 taagctgcct gcctgggtga tgaaaggcgg tacttatact cccgcacaaa ccgtaacgtt
     3961 gggagatgat acgtatcagg tgatgagcgc gtgcaaaccg catgactgtg gctcgcaacg
     4021 tatcgctgtg atgtggtccg agaaatctaa tcagatgacg gggctgttct cgactattga
     4081 tgagaaaacg tcgcaagaga aactcacctg gctgaatgtg aacgatgcgc tttcgattga
     4141 tggtaaaacg gtgctgttcg cggcgttgac cggcagcctg gaaaaccatc cggatggctt
     4201 taattttaaa taattagcgg ataaagaaac ggagcctttc ggctccgtta ttcaattacg
     4261 cggcttcaac tttccgcact ttctccggca actttaccgg cttcgtcgcc agctcttccg
     4321 gatcaaagtc atcaacgtta atactgcgca gacggctttc ttcagctttc accagaatag
     4381 cggcttcatc tttatcaatc agccccttcg ccagcgcgtt gtgcgccagt tcatccagac
     4441 gggtaaacgg caggttttta cccagctctt tacagatccg ctgatgaatt gggtcggcgg
     4501 caatcacatc caccagcgcc tcttccagca agccaaccgg attatgctcg ctcggcgtca
     4561 ggtactgacc gcgaccaatg cgggaacggg tggcgttcgg cacttgtaaa atcttcgcca
     4621 ctttatgatc cagcttgtca gaaggtgcca gataatgacg tccggtcggg aagatcacca
     4681 cattcagcag cccggcaacc acgcggttcg ggaagttttg cagtaaatca tccatcgcct
     4741 gttcagcctg atacagcgca tcttgtacgc cccagtgcac cagcggcagg tcggcttcat
     4801 tacggccttc gtcgtcataa cgcttcagca cggcagaggc gaggtagagc tggcttaaaa
     4861 tatcccccag acgggccgag atgcgctcgc gacgtttcag gctgccgtcc agcactgcca
     4921 tcgagacatc agaaagcagg gcgaggttgg cgctcaggcg gttcaggtgc tgatagtagc
     4981 gtttagtggc atcgccggtt ggcgtgctgc tggttaaacc gcgcgtcagg cccagccaga
     5041 agctgcgaac tttgttgcta ccgacgtgac cgatatgttt gaacaacagt ttatcgaacg
     5101 cgttgacgtc attgttcttc gccgcttcca tctcttccag cacgtacgga tggcaacgaa
     5161 tcgctccttg tccgaagatc atcatgctgc gggtcagaat gttagcccct tcaacggtga
     5221 tggcaatcgg tgcgccctgg taagcacgcg ccaggaagtt gctttgcccg agcataatgc
     5281 ctttgccacc agtgatatcc atcgcatcaa taatcgactg ctgcccgcgg tgggtacagt
     5341 gatacttaac gatagccgac agcacggcag gtttttcgcc gagcataatg ccgtaggtaa
     5401 tcagcgatgc cgcagcatcc atcacgtagg cattaccggc aatacgcgcc agcggctctt
     5461 caatcccttc catcttaccg atagagattt tgaactgacg gcgaatgtga gcatacgcgc
     5521 ccgttgccag cgctaccgat ttcacgccgc cggttgagtt ggaaggcagg gtgatgccgc
     5581 ggcctaccga gaggcactcc accagcatcc gccagccttg cccggccatt ttcggcccac
     5641 cgatgatgta atcgatcggt acgaagacat ctttaccgcg cgtcggtccg ttctggaacg
     5701 gtacgttcag cgggaagtgg cgacgaccaa tttccacgcc cggcgtggtg gtcgggatca
     5761 gcgcacaggt aatacctaaa tcttctgcgc cacccagcag cttttctggg tcggagagtt
     5821 taaacgccag cccaagcaca gtcgcaatcg gtgccagcgt aatatagcgt ttgttccagg
     5881 tcagacgcat ccccagcacc tgctggccct gccattcgcc catgcagaca atcccggtgt
     5941 ccggaatcgc gcccgcatcg gaacccgctt ccgggcttgt cagtgcaaag caagggatct
     6001 cctgaccacg cgccagacgc ggcagatagt gatctttctg ctcgtcagtg ccgtaatgtt
     6061 gcagcagctc gcccgggcct aatgagttcg gcacgccgac ggtaatcgcc aggatcccgc
     6121 tcacgccgga gagtttttgc agcacgcgag actgggcata agctgagaac tccagtccgc
     6181 cgtactcttt tttgataatc atcgcgaaga agcgatgctc tttaaggtac gcccacaact
     6241 ccggcggcag atccgccagc tcatgagtaa tctggaaatc attcgccatc cggcaggctt
     6301 cttctaccgg gccgtcgaga aacgcttgct cttcggcggt caggcgcggc tgcggatagt
     6361 tatgcagctt tttccagtcc ggcttgccct ggaacaagtc gccctcccac caggtggtgc
     6421 ccgcatcaat cgcttctttc tcagtgcgcg acatcggcgg catcacctta cggaaaccgc
     6481 gaaataccgg cgcggaaatc atcgacttac gcataggcgc aaagttaaat ggcacgagga
     6541 taatggccag aggcaccagt acccacgctg accacagacc agcaacgccg agggcggctg
     6601 tccaggcgag caaaatcaga ctgctgataa ataagctcac gcggtgatag aacaacgcgc
     6661 cgagcaggac aaccgtagcg agaatactca aaatcatcat aacgaaaagc cccttacttg
     6721 taggaggtct gaccacttgt gatgatatgg ttgtagtgga tgtaaaaaca tttagcaatg
     6781 tgtttacaat ataattacaa caaagctcac attgttgctg tttttatccg cacttcaggt
     6841 caaaaagtcc tggtcatagc acctgcccgt acttctcgct tttggcggta tccggtacac
     6901 tgcattttgt ctattacatt tatgctgaag gatatcctca tgtaccagga tcttattcgt
     6961 aacgaactga acgaagcggc ggaaacgctg gctaactttt taaaagatga cgccaatatt
     7021 cacgccattc agcgcgcggc ggtcctgtta gcagacagct ttaaagccgg tggcaaagtg
     7081 ctttcctgcg gcaacggcgg ttcccattgc gacgctatgc actttgccga agagttgacc
     7141 ggtcgctacc gtgaaaaccg tccgggctac ccggcgattg ctatttctga cgttagtcat
     7201 atttcctgcg tcggtaatga tttcggtttc aatgatattt tctcccgcta cgttgaagcg
     7261 gtaggtcgcg aaggcgatgt actgctgggg atctccacct ccggtaactc tgcaaacgtg
     7321 atcaaagcga tcgcagcggc gcgtgagaag gggatgaaag tgatcaccct gaccggtaaa
     7381 gacggcggta aaatggctgg cacggcggat atcgaaattc gcgttccgca ctttggttat
     7441 gccgaccgca ttcaggagat tcacattaaa gtgatccata tcctgatcca gttaattgaa
     7501 aaagagatgg ttaagtaagt ctggcgtagg ccggataagg cgtttacgcc gcatccggca
     7561 cttgtgcgct gatgcctgat gcgacgctga tgcgtcttat catgcctaca aatctgtacg
     7621 cgaaccgtag gccgaataag acgtttacgc cgcatccggc ctgaaaattc ttaaatcaat
     7681 cttcgccggg ggccatgcgc tcccgctgtt gtggaggtta cccatgtgcg aattgctcgg
     7741 gatgagcgcc aacgtcccta ccgatatctg ctttagtttc accggtctgg tgcagcgtgg
     7801 tggtggaacc gggccacata aagatggctg gggcattacc ttttacgaag gtaaaggctg
     7861 tcgcacattt aaagatccac aacccagctt taattccccc atcgccaaac ttgtccagga
     7921 ctacccgata aaatcctgtt cggtggtggc tcatattcgc caggccaatc ggggcgaggt
     7981 ggcgctggaa aatactcacc cgtttacccg tgagttatgg gggcgtaact ggacttatgc
     8041 ccataacgga caactgacgg gctacaaatc actggaaacc ggcaacttcc gcccggtcgg
     8101 tgaaaccgat agcgaaaaag ccttctgctg gctcctgcat aaattaacgc agcgttaccc
     8161 gcgcacaccg ggcaacatgg cggcagtgtt taaatatatc gcctcactgg cggatgaact
     8221 gcggcagaag ggcgttttca acatgctgct ttctgacggg cgctatgtga tggcgtattg
     8281 ctcgactaat ttacactgga tcacccgccg cgcgccgttt ggcgtggcaa cgctgctgga
     8341 tcaggatgtg gaaatcgact tcagctcgca gaccacaccg aatgatgtgg tcacggtgat
     8401 tgcaacacag ccgctgacgg gcaatgaaac ctggcaaaag attatgccag gcgaatggcg
     8461 cttattttgc ctcggggagc gtgtagtttg atgccagttg tggctgcaca acttcgtggc
     8521 ttaacggctt gctgaccacg taacgaccat tgaccacaga aacggtcggt ggcttacggg
     8581 tttgctcaaa gtagtcgtaa cccggcttca gttgctccca gaagtcctta aagttggaat
     8641 atttatggcg cttcatattg gcgtcggtca tgcggaacgg gtaaatactc acttgcacgc
     8701 tcggctgacc aaacaccagc gcaccagtaa cgaactggaa gatctcatca ataccctgat
     8761 tggtcattgc gtagcagccg atggaaacac aatcgccgtg gatcatcagg tatttccctt
     8821 cataaccatg cgcacggtca taggcattgg ggaaaccaat attaatcgct ttgtagtaac
     8881 ggctgtctgg ttttaactga ttacgctgga cgctataaaa cccttccggg cttttgaaat
     8941 cgccctgacg ctgttttggc cctaagccgc cggaatattt acagatttta taactgtcga
     9001 gcagctgata ttgctcgccc attttgacgt agagatcgag cgtacgttct tccttgaaga
     9061 tctggatgta gacaggggat cccatcaact gctgcttata ctctttgctc actggcgtgg
     9121 tcgagctact gctgcccagc aaaccggcaa acgaaacgca cgggatcaac aacatcgcaa
     9181 gaattaatgc gattttacgc atactgctta ttccttgata aaacggttac acacgccagg
     9241 acggcaaaat agatcccaaa tcggaatagt ctggatttgg aaggctcaca ttatcaccaa
     9301 aagagtttta cgcaagcctg tcggcgcagg gtttacaaat ttcatcggca gcgtgcttta
     9361 cccaaagagc gccgcgtgag ttccagttct ctcaaatcgt aaaagtttat cggtaagttt
     9421 gtaaatcagg atccagtccg gttcgacatg agcatcgcga taacctttcc atgaaccttg
     9481 cagcgggtgg tctttataaa cagctggaag cggtaaagca ttattgataa gaagcgtcat
     9541 aagatatttc aatttattca tatccttatg acgcttttgt gcaagtttta catcctttga
     9601 aaattgtccc gagtattcaa tatccctttg aatcatattt aaattcctaa tttatcaaat
     9661 aaatcatcag cgtctttggc cttatgaaca tcaacgccag cttcgctgtt tttgattgat
     9721 tgaatggtta attgattagg ctcgcgtaaa tcaaacggca atgccttttc acgcgcgacc
     9781 tttgtgaggg ttatgcgaac caggtcagag atggtcagcc ccatcccggc cagtacgtcc
     9841 gccgcctgat tcttcagatc ttcatcgatt cgggcgcgaa caaacgcgtt agcagccatt
     9901 gggggatctc ctgtgctgta tatttattca gcttgaattg tagctcaaat gagcaacagc
     9961 gacaatccat gagtaattta ataaacggat gtttttacat aataaactga aatatattta
    10021 ttaattttat aataatggtt tattaagttt gttcaggtac gggccaggat acactgcaac
    10081 atcaatgcat gaccagccat tattgagaat gtctttgccg tcgattccct cttttgtgtt
    10141 gtcgggatta ctgttgcttt gcttgccgtt ttcttcattt gcctgcgcca ccacatcaca
    10201 tatctctttc agctacgccg cccgccagcg gatgcaaaac cgtgcgcgtt tattaaaaca
    10261 gtaccaaacc catctgaaaa aacaggccag ctacattgtg gaaggcaatg cccaaagcag
    10321 aagggcgctg cgccagcaca accgggagca gataaaacag catccagaat ggtttcctgc
    10381 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc actttttaag
    10441 cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgtctggagc agcagcttgg
    10501 caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt
    10561 tttttatgcc tacaacaaga tcctcgaggc gaagctcccg cgtacggcca atgagatgta
    10621 tcactaccgt cgggcaacca ttgtggcgaa taacgacctg cgccggggag atttgctgtt
    10681 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
    10741 gtttattgag tcaccgcgca ccggggaaac cattc
//
LOCUS       DAJVXP010000134        10716 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVXP010000134 DAJVXP010000000
VERSION     DAJVXP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.134"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..1093
                     /gene="dacC"
                     /locus_tag="OQS10_003352"
                     /pseudo
     CDS             1..1093
                     /gene="dacC"
                     /locus_tag="OQS10_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="OQS10_003353"
     CDS             complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="OQS10_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS2294301.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="OQS10_003354"
     CDS             complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="OQS10_003354"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS2294302.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2837..4064
                     /gene="mdfA"
                     /locus_tag="OQS10_003355"
                     /pseudo
     CDS             2837..4064
                     /gene="mdfA"
                     /locus_tag="OQS10_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4105..4389)
                     /locus_tag="OQS10_003356"
     CDS             complement(4105..4389)
                     /locus_tag="OQS10_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294303.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="OQS10_003357"
     CDS             complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="OQS10_003357"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS2294304.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5290..6498)
                     /locus_tag="OQS10_003358"
     CDS             complement(5290..6498)
                     /locus_tag="OQS10_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2294305.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6582..7118
                     /gene="rcdA"
                     /locus_tag="OQS10_003359"
     CDS             6582..7118
                     /gene="rcdA"
                     /locus_tag="OQS10_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS2294306.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7293..8978)
                     /locus_tag="OQS10_003360"
     CDS             complement(7293..8978)
                     /locus_tag="OQS10_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS2294307.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9248..9625
                     /locus_tag="OQS10_003361"
     CDS             9248..9625
                     /locus_tag="OQS10_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS2294308.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="OQS10_003362"
     CDS             complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="OQS10_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS2294309.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10072..10359
                     /locus_tag="OQS10_003363"
     CDS             10072..10359
                     /locus_tag="OQS10_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS2294310.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10343..>10716
                     /gene="nfsA"
                     /locus_tag="OQS10_003364"
     CDS             10343..>10716
                     /gene="nfsA"
                     /locus_tag="OQS10_003364"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS2294311.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2649 c         2714 g         2588 t
ORIGIN      
        1 gatgcgcgtg catggatttt aatggattac gccagcggta aagtgctggc agaaggcaac
       61 gcggatgaga aactggatcc cgcgagcctg actaaaatca tgaccagcta tgtggttggg
      121 caggcgctta aggccgataa gattaaactc accgatatgg tgacggtcgg taaagatgcc
      181 tgggcgacgg gaaatccggc actgcgtggt tcatcggtaa tgttcctcaa accgggcgat
      241 caggtttcgg tggcagactt gaacaaaggt gtgattatcc agtccggtaa tgacgcctgt
      301 attgcgctgg ccgattacgt tgccgggagc caggagtcat ttattggttt gatgaatggt
      361 tatgccaaaa aaactgggtc tgaccaacac taccttccag acggtgcacg gtctggatgc
      421 gccggggcag ttcagtaccg cgcgcgatat ggcattgctg ggtaaagcat tgatccacga
      481 tgtgccggaa gagtacgcca ttcataaaga gaaagagttc accttcaaca aaattcgtca
      541 gcctaaccgt aaccgtctgc tgtggagcag caatctgaat gttgatggca tgaagacagg
      601 aaccacggca ggtgcgggat ataatctggt tgcttcggct acccagggtg atatgcgttt
      661 aatctccgta gtgctggggg cgaaaactga ccgtatccgt tttaatgagt ctgagaaatt
      721 attgacctgg ggtttccgct tctttgaaac tgtgacgcca attaaacctg atgccacctt
      781 tgtgactcag cgcgtctggt ttggtgataa gagcgaagtg aatctcgggg caggcgaagc
      841 gggctccgtg accataccgc gtgggcagct gaaaaacctg aaggcgagct acacgttaac
      901 ggaaccgcag cttaccgcac cgctgaaaaa aggtcaggtt gtcgggacca ttgatttcca
      961 gcttaacggt aaatccattg agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg
     1021 cggattcttt ggtcggatgt gggatttcgt gatgatgaaa ttccatcagt ggttcggcag
     1081 ctggttctct taatcttctg ataaccggat ggcgcgaaac gtcatccggt tatacgtcat
     1141 taatacatca acttaatacg gtgcgtctgc gcgtacttca catactcatc ttccgggcaa
     1201 caatcactca ccacaatatc aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg
     1261 ccaaatttac tgtggtcgac aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt
     1321 tttaccggca actcttcaag attaaaacag gtagcgcctt tactgacatg cacgcccgcc
     1381 gcggaataaa aagcgatatc cgggcaaaaa ttattcaacg tttgctgaaa atcgatgggg
     1441 ttgaaaatgg cgttgctggc gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt
     1501 ttctctttca gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa agggatttca
     1561 ttatcaatcg cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga
     1621 tcgggttcta ccagtgtcgc agccagtttt gccgcccggc gtttttcttc caccaggcgg
     1681 gatttttgat cgcttaacag gtaatggctg gcactgcgcg gttccagaac aatatagccg
     1741 ccgagcaaaa cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt catctccgaa
     1801 accccaagca gggcggcggc gtctttaaga tgtaacttat cgctgcgttt taattcttgc
     1861 agcagctgcc cgatacgctc ttcgcgacgt gtttccataa tccctctgaa tagttattga
     1921 agcgagccgc tcaatactac actttttagc agagatcagt cacgcaccca gcctttgcgg
     1981 atcggtaatg caaaacagac gcgataccac gattgcagac gttgatagag tttatgcccc
     2041 atcgcttgcc agataatctg ggcactaagg cagccaatca tacctgccag caatccaccg
     2101 agcatatcca gcggccagtg gacgccaaga taaacgcgcg accaggcaat gacgacggcc
     2161 agaaccatta aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag
     2221 gtgaaaatca ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg atgcaggaag
     2281 ttatagccga tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag
     2341 gacacaaaca ggctgaccgc cagcgcgata gcgattttta tcaccagttg ccgttgtgct
     2401 gtaagccccc acaaccaaag taccgcggcc agcaacggca ccacggtaat caaatcttta
     2461 gcaataaaaa tcgccaacga gatcatccac ggagccgagt ctggcgtcgc gttaataaga
     2521 gagaatagag agagattcaa attttccagc ataacttccc gacgcaaagt gattaaaagg
     2581 gagccaatac aggcaagtcg ttgagaataa agtgcaggtt aaactgggta aagcggcatc
     2641 gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt gtaacgggca taggttaaat
     2701 aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt
     2761 tcagctacaa aattcgcttt ctcgttagct gcgcttttat taaactctgc gcgattatta
     2821 ttggcgaaga aattgcatgc aaaataaatt agctttcggt gccaggcttg gacgtcaggc
     2881 gttacttttc cctctctgtc tggtgcttta cgaattttca acctatatcg gcaacgatat
     2941 gatccaaccc ggtatgttgg ccgtggtgga acaatatcag gcgggcattg attgggttcc
     3001 tacttcgatg accgcgtatc tggcgggcgg gatgttttta caatggctcc tggggccgct
     3061 gtcggatcgt attggtcgcc gtccggtgat gctggcgggt gtggtgtggt ttatcgtcac
     3121 ctgtctggca atattactgg cgcaaaacat tgaacaattc accctgttgc gcttcttgca
     3181 gggcataagc ctctgtttca ttggcgctgt gggatacgcc gcaattcagg aatccttcga
     3241 agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac gtggcgctga ttgctccgct
     3301 acttggtccg ctggtgggcg cggcgtggat ccatgtgctg ccctgggagg ggatgtttgt
     3361 tttgtttgcc gcattggcag cgatctcctt tttcggtctg caacgagcta tgcctgaaac
     3421 cgccacgcgt ataggcgaga aactgtcact gaaagaactc ggtcgtgact ataagctggt
     3481 gctgaagaac ggccgctttg tggcgggggc gctggcgctg ggattcgtta gcctgccatt
     3541 gctggcgtgg atcgcccagt cgccgattat catcattacc ggggagcagt tgagcagcta
     3601 tgaatatggc ttgctgcaag tgcctatttt cggggcgtta attgcgggta acttgctgtt
     3661 agcgcgtctg acctcgcgcc gcaccgtacg tgataattat gggcggctgg ccgattatga
     3721 ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga
     3781 ctgccgggtt aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat
     3841 taaccctgtt tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc
     3901 aaatgctgat ctttaccgtt ggtattgaaa tcagcaaaca tgcctggctg aacgggggca
     3961 acggacagtt taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta
     4021 tctttttaaa agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgactgc
     4081 tttgtgcgat caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac
     4141 catcccttca cctacgctgg caagatggcg agcataagga tgcgggcggt aagccggagc
     4201 tggcgcagga gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac
     4261 gctgactgaa tcaggaacta caaccacttt gtatccactc ggcacatcga cggtgacact
     4321 ttgcgccatc gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt
     4381 cataatcatt tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca
     4441 ttcaggtatt gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt
     4501 taagcacttt atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt
     4561 ttgccgctgc gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac
     4621 gcagcatctc aatatcgtta ccgccatcgc caaagaccac cacttcgctg tcgtctattc
     4681 cccataattt ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat
     4741 cgatgctgcc gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg
     4801 ctttttgtac ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat
     4861 cttctaagtt gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tctgccaccg
     4921 ttttcatggc atcgtcatat tttttgagcg tataggcgct attttttccg caggcaataa
     4981 tttccacttc cgggcgcgtc agcaaatgtt ccacgacagt agtaaaggca tccttcgaca
     5041 gctcgccatt aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa
     5101 aggcaatttc attagcaatt tcagggaaga aagagagcaa ctgataatat tgattcccgc
     5161 tggcgaccac aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct
     5221 cacggttata ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt
     5281 taatgctcat caactattct ccatcgccgt ccgcgttttg gtatcgggtt tggcgacggc
     5341 tttcgcgaca atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa
     5401 tccataatgt tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc
     5461 ggtcgttgct accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga
     5521 aatggtcagc gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc
     5581 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact
     5641 ggcccgaacc acggcaacgc gactgtaacg gtcgatgaac caaccgccag tgaagcgtcc
     5701 aacggtcatc cctagggtaa aaccggcata aatcagcgag ccggaagtag ggctaaaacc
     5761 gtgaccgtca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
     5821 cgccagcact acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgccttt
     5881 ttcgccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
     5941 ggcgatataa ataggcgcga tacctaccag cgccgccaat aaaatgtgca ccgttgccgg
     6001 aacgccaaag gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ctaggctata
     6061 aaaaccgtgc atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc
     6121 aacgtttatc gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc
     6181 aaagagcagg ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgatcaatgc
     6241 gcaggacatc gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga
     6301 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
     6361 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
     6421 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
     6481 tgaagaattt acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag
     6541 gttgtgtaca tttgtacaca attgcagaca gaggaaatga catgcgtcgc gctaacgatc
     6601 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
     6661 atgctgttac gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct
     6721 actatttttc aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatta
     6781 tgtcccggca atatcaggca ttttttagcg atgttagtga tgctcaaggc gcatgccagg
     6841 ctatcaccga tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt
     6901 accagctcta cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga
     6961 tgcagcgcag tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
     7021 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg
     7081 aggagatttt gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa
     7141 aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
     7201 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
     7261 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
     7321 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc
     7381 agcgcaggga tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg
     7441 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
     7501 aacaagaaac agataaccac gggcacaaga ctgacaatta atccggcaat caacatctga
     7561 ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
     7621 ataaacacca tcaagccgaa ctctttcacc atgcttaatg caccttgcgg aatgtaaccg
     7681 aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca
     7741 gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc
     7801 ccaataacaa agaaggcgca aaatgccagc aggtcagtga cctggctgtg aatcgagata
     7861 aagccgatgc gatcggcgat ggtttttacg cgacgggcat caccgctgac ttgtaaaacg
     7921 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
     7981 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt acctacagcg
     8041 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
     8101 aaaacttctt taccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct
     8161 accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc
     8221 ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc
     8281 agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc
     8341 ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg
     8401 gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa
     8461 ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt
     8521 gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc
     8581 ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac
     8641 agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt
     8701 aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg
     8761 acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt
     8821 tgttggccta ataacagcga tacgactaaa acgccaatgg aattacccag ttggatcgaa
     8881 ccaagtcgta actttccgag acatagccca agcgtgagga ccacaaataa taacagaatg
     8941 taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact
     9001 agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc
     9061 agattcattc agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca
     9121 cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga atgcgaagcg
     9181 gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga
     9241 ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat
     9301 tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc
     9361 tgaaatcggc ttgctgtttt tcattcttcc tggagcagtt gccagtttct tttcacagcg
     9421 tagagaagtc ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt caatgctcat
     9481 tatgcggctg tttttttcac cgacgcgctc attctggcaa gagctggcat ggttactaag
     9541 cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa
     9601 accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc
     9661 gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc
     9721 gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt
     9781 agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct
     9841 caatttctca gccagatctt ttgcacgcac acagtaaggg caacccgaac gaccaaaaat
     9901 aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt
     9961 attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca
    10021 caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg
    10081 atcggtaaat tgcctaaagg cgtgttgata ctggaattta tcggaatgat gctactggcg
    10141 gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg agccattttc tcggccagaa
    10201 gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt
    10261 attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca
    10321 cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gccatcgctc
    10381 cattcgccat ttcactgatg aacccatttc cgaagcgcag tgtgaggcga ttattaacag
    10441 cgcccgtgcg acgtccagtt ccagtttttt gcagtgtagt agcattattc gcattaccga
    10501 caaagcatta cgtgaagaac tggtgacgct gaccggcggg caaaaacacg tagcgcaagc
    10561 ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc
    10621 tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca
    10681 gaatgcatta accgcagcgg aatcgctggg attggg
//
LOCUS       DAJVXP010000135        10687 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVXP010000135 DAJVXP010000000
VERSION     DAJVXP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.135"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..785)
                     /locus_tag="OQS10_003365"
     CDS             complement(<1..785)
                     /locus_tag="OQS10_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294312.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            958..1836
                     /gene="yihU"
                     /locus_tag="OQS10_003366"
     CDS             958..1836
                     /gene="yihU"
                     /locus_tag="OQS10_003366"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS2294313.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1861..2748
                     /locus_tag="OQS10_003367"
     CDS             1861..2748
                     /locus_tag="OQS10_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS2294314.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2781..3791
                     /locus_tag="OQS10_003368"
     CDS             2781..3791
                     /locus_tag="OQS10_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS2294315.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3861..5294
                     /locus_tag="OQS10_003369"
     CDS             3861..5294
                     /locus_tag="OQS10_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2294316.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5393..6742
                     /locus_tag="OQS10_003370"
     CDS             5393..6742
                     /locus_tag="OQS10_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS2294317.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6732..7619
                     /locus_tag="OQS10_003371"
     CDS             6732..7619
                     /locus_tag="OQS10_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS2294318.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7760..8359
                     /gene="yihX"
                     /locus_tag="OQS10_003372"
     CDS             7760..8359
                     /gene="yihX"
                     /locus_tag="OQS10_003372"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS2294319.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8353..9225
                     /locus_tag="OQS10_003373"
     CDS             8353..9225
                     /locus_tag="OQS10_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS2294320.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9222..9659
                     /gene="dtd"
                     /locus_tag="OQS10_003374"
     CDS             9222..9659
                     /gene="dtd"
                     /locus_tag="OQS10_003374"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS2294321.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9704..10645
                     /gene="fabY"
                     /locus_tag="OQS10_003375"
                     /gene_synonym="yiiD"
     CDS             9704..10645
                     /gene="fabY"
                     /locus_tag="OQS10_003375"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS2294322.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2532 c         2854 g         2839 t
ORIGIN      
        1 tcttttgttg gcattttttc tgccaacgct accgcgagtg cgccgtgaaa aacatcgcct
       61 gctccagtgg tatcaaccac attgacagaa aatgcctcct gttgacacaa atgaccatct
      121 tcaatccaca acgatccttc agaacccagc gtaacataca ctttacctgc tgtttgtgtc
      181 gttgcctgga acaaaccttc ttcaggtgac tgcaggccag tcatgcgttt cagccctggt
      241 gttgagaaca cggcgtgatc ggcaagcgcg acaagtggag aaatatcctg cggtgtcata
      301 tcggcgtcta aaagcgttgt cacacccgca aggcgcgcta gcgagaatgc tttttcagta
      361 ccgctatgcc agcgaacatc ggccagaatc agatcataac gagagaaatc gatcgcttct
      421 aaccattcgg catcagtacc gagatcagga cttggataat taacgataat ccgttcgcca
      481 tgctgatcaa cgaggatcgc tgattgcgac gaacgggcgt taggatatcg gcggcaaaat
      541 gcggtattga cgccccaacc ctcaagttct gcaagtaagg tatttccaca actgtcatcg
      601 ccgacgcgac cgatgaaatc aacctccacg ccaagctttg cgattgcaac agcggctgtt
      661 gctgccgggc cgccaccaat ctcaagataa tgatttgcct gatatttccc gccgccctcc
      721 ggcagagtgg gcaaagaata aattctgtcc tgaactgtga tacccacgca tgcaattctg
      781 gtcatatttg tcttgtcctg tttaattttg tcgtctttgg tcaatttaaa tgacgcaaat
      841 gatcaaatta gtgatgcgtg tcgtgttttt gaacataaat tacaaatatg ctcaaaaaca
      901 gacgaaaacg aattttcaat tgtcattatt ttgtcaatgt cagacaggag agtattcatg
      961 gcaaccattg catttctcgg gctggggcag atgggtagcc caatggctaa caatctattg
     1021 caaaaagggc attcattgca ggtatttgat gtcaatgcac aggccgtcga tgcgctagtt
     1081 acgcaggggg ccactgcggc acaaacccca gcggaagcag cagcaggcgc tgaatttatc
     1141 atcactatgc ttcccaatgg tgacattgtt cgacaggttc tgttggggga aaaaggcgtc
     1201 tgtgaggctg tttcatctga cgcgttattg attgatatgt cgactattca tcccttacag
     1261 accgatgcat taatacggga gttacaagaa aaaggcatca gtatgatgga cgctcccgtg
     1321 ggcagaacgt ccgtgaatgc gattgatggc acattgctga ttttagcggg tggcactgat
     1381 gagcagattg cacgtgcacg cccgattctc atgtgcatgg gtaatgagct ggtcgaggct
     1441 ggcggcccag gaatggggat ccgcgtgaag ctaattaaca actatatgag tattgcgctt
     1501 aatgcgctgt cctccgaggc cgctgtgtta tgtgagtcac tgggattaaa tcttgatgtc
     1561 gcaattaagg tgatgagtgg tacggcagca gggaaaggac attttacgac cacctggcct
     1621 ggcaaagtgc tgaaaggcga tctttcaccc gccttcatgg tggatctggc gttgaaagat
     1681 ctgcgtattg cagttgacgt cgctcgggag acgggggcac cgctcaatat ggggattgca
     1741 gcagaaagtt attacgctgc agcaagtcaa gacggcaaag ggcggcagga ctggagtgcc
     1801 ctgctaaatc aggttcgcca gcaggctggt ctttaatttt atctattaac aggagtttct
     1861 atgtcatccc acttcacccc atttacgctg aaagatatct cccgcccagg tggtggattt
     1921 gccatgttag ctgtcgatca gcgtgaggcc atgcgcttga tgtttgcggc agcagggcag
     1981 ccaaaaccca tcgctgacag tgtactcacc gattttaaag ttgccgcgac ccgaatcctt
     2041 tctccttatg cttctgccgt tctggcggat aaacaatttt gtcttgagca aatagtcgag
     2101 cagggcgctg ttgctaattc ttgcggcctg attgtcgctg ccgatctttt tatcccaggt
     2161 aatggcatcc cggttgatag cgttgaaata gatatgtccg tcgacccgca taaagcccgt
     2221 gaaatggggg ctaaggcaat gaaattactt gtactctggc gtgaagatga acccgcagag
     2281 gaacgtctgg caatggttga taaatttgtt cgtcgctgcc gtagtgccgg gctggtgagc
     2341 attattgaac ctgtggtgcg tccacctcgt cgcggttggg actttgatcg tgaaagcacc
     2401 attgttgccg ccgccgcaga gcttggtgga acagaggctg atctttacaa agccgagatg
     2461 ccattagggg gcaaaggcga tgaaaaaacc ttacttgccg cctgtcagca acttaacgat
     2521 cagatgaaga tgccgtgggt aattctttct tctggcgttg atgccgatat ttttggccgt
     2581 gcagtaagta ttgccatgaa aggtggtgcg tcgggcttcc tggctggacg agcggtttgg
     2641 gcgtcggttg ttggtgcaca agatccacaa actatgctgc gcgatgtctc tgttcctcgt
     2701 ttacaacggt tggcagaaat tgtcgatgaa ggtatcgcgc aacgctaatc ccctatggat
     2761 atcgattctg gagaataaca atggataaaa ttacccaggt tttattttca gatattggga
     2821 aagtcaccac tcaatatgtt gaagtaccac accaggaact taaaccgcac gaagtgcgga
     2881 ttgcgcctgt gttctatggg atatgcggtt cggatctgca tgttctgaaa ggcggtcatc
     2941 cgtttgccaa accacctgtc gtccccggtc atgaaattgc agcgcgcgtt acggaagttg
     3001 gcagcgacgt taaaaatgta cagccgggcg atcatgttgt ggtcgatccc atcatggctt
     3061 gcatggaatg ccgagcctgc aaagcaggac gttttaatct ttgtgaacca ccgcaggttg
     3121 ctggttttcg cgcaccgggc tttgctcgct cacaacacat tgttcctgcg cgtaattgcc
     3181 atgtcgcacc agcctcttta ccgctaaaaa tgttggcctt tgccgaaccg gcggcttgtg
     3241 cccgtcactg cgttaaccga atgccgaaag cttctctgga aagcgtactg gtaattggtg
     3301 ccggaacgat aggcttatcc atcgtgcagg cactgcgcat tatgggggca ggtaagatta
     3361 ccgtgattga acctgacgct gccaaacgcg cgctggcgtt aaagctgggc gcagcagaag
     3421 tttgggcacc aggtgagctg gccgcagatg tgcgatttac gggggcgatt gatgtcgttg
     3481 cagcgcaggc cacgcttaac gatgcatgta cccgtgtata tgccggaggc accgtcgtgt
     3541 gcatgggcgt accaagtggg ccgcgtgaaa taccattacc gatgatgcaa cgtttcgagc
     3601 gtgacttgct caactctggc atgtacatcc ctgaagattt cgatgctgtt atcgaatggc
     3661 tggcggatgg gcggtttgat accagtgaac tggttaccga tttatttgcc attgaggatg
     3721 cagcggcggc atttgaacgc gcgcagcaaa atgactccat aaaggtcatg ctgcaatttg
     3781 cgccggaatg attttctaaa tctctgtctg gcgctgcgtc acgtcagcgc caccctacaa
     3841 caattaaaaa ggacactgcg atgagtcaaa tcaccagtcc tgcaacttat tcaattagtc
     3901 ggccacagga cgtaattgat attgttaata agaactctgc aatcaacacc agtatcggcg
     3961 ttatttttat tgctttgggc ggcatattga ttgatgccta ccaggcggcg atggtggggt
     4021 ttggtaataa atacattgcc gctcaattcg gcatttcgcc gggtcttgct gcaaccgtca
     4081 atgcatcagt attaatcgcc gcgttaattg gcggtttatt agcgaaccga gtaataaacc
     4141 gctttgggca aaagcgggca tttattattg gcatggggct gtgcaccatc ggtgctgcag
     4201 cggtagctat tgctcccagt atctggtggg tgctggtgtg ccgcgtcatc atgggctttg
     4261 gtttaggcat cgacttccct ttggcaacca atgccgtggc agagcttcgt ggttcaacgt
     4321 cgaagaaaac cggaacgtcg gtcaacctct ggcaaatggc ctggtatgtt tcgacaaccg
     4381 ttgtttattt ggtacttttg ccgctgcttc tgtcgggtat cgctgaagaa caattgtggc
     4441 gttacggaat attcatcgga gctatttttg cagtcatctt catgattttg cgttacttct
     4501 ttattggtga atccgcaatg tgggccgcac gcgtcgggcg ttaccaggaa gcgtgcgaca
     4561 ttctgggaaa acgttatggt gttcaggctc gcgttgcggc atcgggtaca acagaagcga
     4621 aattctcgga aaaagcagag aataaataca gtggtggata tggcatctta tttaatgatc
     4681 gttaccgcaa acgcaccatt cttggctgtg tcgtggcaac catgcaggcg tggcaatata
     4741 acgccgtagg tgtttatctt cctcttacgt tggcgggaat aataagtggc gggcttactg
     4801 gcgcgttgac cggttctgcc gtcgtgaatg ccctttgtgg ggtgacaggc gggatgatcg
     4861 gctcgtttat tctccaacga ctgggtactc gccgacagtc gatgtatgga tttgctgttg
     4921 tgaccttagc attgctgtcg ttaggcgcac tggcaacgac taatccctgg ctgtctttag
     4981 ggttattggg atcaattatt ttcttccatt cagcgggtcc tggtgggctg ggcatgacca
     5041 ttgccacact ctcttatcct cccgctattc gccctactgg ggtcggattt gcccgcgcta
     5101 ttatgcgcac aggggcaatt gcaggactca ttttctggcc gatgctgtgg ggtgcgttga
     5161 aaactgaagc gttttactgg ttggcaatcg tgccattcct ggggttcctg acctgcgtat
     5221 tgattaattg ggaaccactg ggtgcaaatg ttgatgctga ggatgcagag gttctggctg
     5281 aattgaagaa ataaattaat gcgcatcggc ttaaaccgat gcgcattaat tcaaactgcc
     5341 cacgtttagt ggttcgtatc ccagatttct cgcctaccag gagaggaaaa ttatgtctga
     5401 aaagttaccc gcaccgcgtg aagggctttc cggtaaagcc atgagacgtg tcgttatggg
     5461 cagtttcgca ggcgcgttaa tggaatggta tgacttcttt atttttggca cggcagcagg
     5521 actggtattt gcaccgctgt tttatccaga cagcgatcct tttataggtc ttatagccgc
     5581 ctttgcgacc tttggtgtcg gttttctgac tcgtccttta ggtggcatcg tctttggtca
     5641 ttttggcgat aaaatcgggc gaaaaataac acttatctgg acgctggcta ttgtcggttg
     5701 ctccacattt ttgattggtt ttattccgac gtatcaggaa attggcattt gggcaccgct
     5761 tgtattaatg gtgctgcgtt tgattcaggg ctttggtctt ggtggcgagt acggcggagc
     5821 ggcattgatg accatagaat cagcgcccga atcccgacgc ggatttttag gttcactccc
     5881 gcaaacggct gcgtctgtgg gtattatgct ggcaaccggt atttttgctc tctgtaacca
     5941 ttttcttact tcggagcagt ttctctcctg gggatggcgc attccgtttt ggctttctgc
     6001 ggtgatgttg atcgttgggc tgtttatccg cctgcatacc gaagaaacac tggattttca
     6061 aaagcaaaaa acgacgaata ataaagaaaa gtccgttcct ccgttgattg aattattcaa
     6121 aaaacatcca cgaaatattt tattggcact gggtgcgagg ctggcggaaa gtgtctcctc
     6181 taatattatt aacgcctttg gtattgtcta tatttccagc caattagcat tgtcgcgaga
     6241 tattcccctg acgggtatgt tgattgcctc ggcgatcggt attttcagtt gtccattggt
     6301 tggatggtta tcagatcgta taggtcaaaa aagtctgtat ttgtcgggag caggattttg
     6361 tgtcctgttc gcgtttcctt tcttcttatt actggatagc aaaagtacgc tcatcatctg
     6421 gtgcagtatg atcctcggct acaacttagg tccgacgatg atgtttgccg tgcagccaac
     6481 acttttcacc cgcatgttcg gtaccaaagt acgatatacc ggcctttctt ttgcttatca
     6541 attttccgcg attcttggcg gactaagccc ccttatcgcc tcaagtttgc tggcgctagg
     6601 gggaggaaaa ccgtggtatg tcgctctgtt tttatttgct gtctcagttc tttcctttgt
     6661 ttgcgtctgg ctgattgagc cgaccgatga acaagaaacc gcttcttacc gctacatcag
     6721 ggaacaatct catgaaaact gaacgaaaaa acgagcatta cctggcgcta caacaggcgt
     6781 ttgacgcgcc atggcctggg cctgtaggag agttagtgac gctggaaaaa ggcaatattc
     6841 acctgcagat ttatccgcat gatggtgcca ggataacctc gttaaaggca tttggttctg
     6901 aagtattacg ccaatggcag ccgcagcgca gagcttttca gtatggctgt ttcccaatgg
     6961 tgccgtgggc agggcgactg gggaatgcga cgctgaatgc tggtgggcaa tgttattctt
     7021 tacccgccaa taaaccacca catgcgttgc atggtatggc ttgttactca acgtgggaaa
     7081 tcatcgacaa aacagcagat tccctgactt tacgtatgcc cctggcttca ccgtggccct
     7141 ggcaagggga agtgattcag acttttttgc tggagaacga tgcgctggtt ttgcaactgg
     7201 aagtccattc atatgccgat acattcccgg cctccgcagg ctggcatccg tggtttgcga
     7261 aaaagctgac tccgcaaaat acagaatcgt tgcaggtgct ttttgatgcg gactggcaag
     7321 aagaagcggg tagtgatgaa ctgccaactg gaaatcgtat ctttcctcaa gccggtccct
     7381 gggatgactg ttttggtttt tataacgggg tgaaggttaa gttactgtgg cctggtaaac
     7441 tcgcgatgac catgatctct tctgcaaaca gtctggtggt atttgataaa cagcccgatg
     7501 ccacctgtat taacccgctg actcaggcac ctaacgcaat aaatctaacg ccggaattgg
     7561 tgacaccaga taggccgctg gtcatcgaaa ctcgttggca atttacacct gaatcttaac
     7621 cattcatcta ttgcgaaaaa gccagtgaaa ttactctgct ttttcgcaat cagcatcaca
     7681 agattaccct tattgccaca tagccaaacc catcctttcc cgctacagtt aatttcttgt
     7741 ggcgcgaaag gaggcaaaaa tgctctatat ctttgattta ggtaatgtga ttgtcgatat
     7801 cgactttaac cgtgtgctgg gagcctggag cgatttaacg cgtattccgc tggcatcgct
     7861 taagaagagt tttcatatgg gggaggcgtt tcatcagcat gagcgtgggg aaattagcga
     7921 cgaagcgttc gcagaggcgc tgtgtcatga gatggctcta ccgctaagct acgagcagtt
     7981 ctctcacggc tggcaggcgg tgtttgttgc gctgcgcccg gaagtgatcg ccatcatgca
     8041 taaactgcgt gagcaggggc atcgcgtggt ggtgctttcc aataccaacc gcctgcatac
     8101 caccttctgg ccggaagaat acccggaaat tcgtgatgct gctgaccata tctatctgtc
     8161 gcaagatctg gggatgcgca aacctgaagc acgaatttac cagcatgttt tgcaggcgga
     8221 aggtttttca cccagcgata cggtcttttt cgacgataac gccgataata tagaaggagc
     8281 caatcagctg ggcattacca gcattctggt gaaagataaa accaccatcc cggactattt
     8341 cgcgaaggtg ttatgctaaa aaccattcag gacaaagcca ggcaccgtac ccgtccacta
     8401 tgggcctggc taaaactact ctggcaacgt attgatgagg acaacatgac aaccctggca
     8461 ggtaaccttg cctatgtgtc tttgctctca ttagtgccgc tggttgccgt tgtttttgcg
     8521 cttttcgccg cttttcccat gttttccgac gtcagcattc agttacgtca ctttattttt
     8581 gccaactttc tgcctgctac tggcgatgtt attcagcggt atatcgaaca atttgttgcc
     8641 aattccaaca agatgaccgc cgttggggcg tgcgggctga tcgtcaccgc gttattgttg
     8701 atgtactcca tcgatagcgc gttgaatacc atctggcgca gtaaacgagc gcgaccaaaa
     8761 atttactcgt ttgccgtgta ctggatgatc ttaacgctgg ggccgctgct ggcaggggcc
     8821 agtctggcga tcagttccta tttgttctcc ctgcgctggg cgagcgatct caatactgtc
     8881 atcgataacg tgctgcgtat ttttccgctg ctgttgtcgt ggatctcctt ctggttgctg
     8941 tacagcattg ttcctaccat ccgcgtacct aaccgcgacg cgattgtcgg cgcgtttgtc
     9001 gctgcactcc tgttcgaagc aggaaagaaa ggtttcgcgc tttatatcac catgttcccg
     9061 tcatatcagc tcatttacgg cgtgctggcg gtgatcccca ttctcttcgt ttgggtctac
     9121 tggacatggt gtatcgtctt gcttggcgcg gaaattactg tcactctcgg ggaataccgc
     9181 aaactaaaac aagcagctga acaagaagaa gacgacgaac catgattgca ttaattcaac
     9241 gcgtaacccg tgccagcgtc accgtggagg gagaagtgac gggcgaaatt ggcgcgggac
     9301 ttttggtgtt attgggtgtc gaaaaggatg acgacgaaca gaaagcaaac cgtctgtgcg
     9361 agcgtgtgct cggctaccgc atctttagcg atgccgaagg caagatgaat ctcaacgtgc
     9421 aacaggcggg cggcagtgta ctggtggttt cccagtttac cctcgccgca gataccgaac
     9481 gggggatgcg cccaagtttc tccaaaggcg catcaccgga tcgcgcagag gcgttatatg
     9541 actatttcgt cgaacgctgc cgccagcaag agatgaacac gcaaacagga cgcttcgctg
     9601 ccgatatgca ggtatcgctg gtcaatgatg gccccgtgac attctggttg caggtatgag
     9661 cctgcttcca gggttgtcac gggaaacaag agagagtatc gctatgtatc accttcgggt
     9721 tccacaaaca gaagaagagt tagagcgtta ctatcagttt cgctgggaaa tgttgcgtaa
     9781 gcccctgcat caaccaaaag gttcggaacg cgacgcgtgg gatgcgatgg cgcatcacca
     9841 gatggtcgtc gacgagcagg gtaatctggt ggcggtaggc cgattgtata ttaatgccga
     9901 caatgaagcg tccattcgct ttatggccgt tcacccggac gtgcaggaca aagggttagg
     9961 aacgctgatg gcgatgaccc tggagtcggt ggcgcgtcag gaaggcgtta agcgcgtgac
    10021 ctgtagcgcc cgtgaagacg cggtggagtt tttcgccaag ctgggattca ttaatcaggg
    10081 ggagatcacc accccaacta ccacgccgat tcgccatttt ttgatgatta aacccgttgc
    10141 cactctggat gacattctgc atcgcggtga ctggtgcgcg cagctgcaac aggcgtggta
    10201 cgaacatatc ccgttaagcg aaaaaatggg cgtgcgcatt cagcaatata ccgggcaaaa
    10261 atttatcacc accatgccag aaaccggtaa tcagaatccg caccatacgt tgtttgccgg
    10321 gagtttattc tcgctggcga cgctcaccgg ttggggactt atctggctga tgctgcgtga
    10381 acgccacctc ggcggaacga ttattctggc ggatgcgcat atccgctaca gcaagccgat
    10441 tagcggtaaa cctcatgcgg tagccgacct tggtgcctta agcggcgatc tcgaccgtct
    10501 ggcgcgcgga cgaaaagcac gggtgcagat gcaagttgaa atctttggcg acgagacgcc
    10561 gggtgcagtg tttgaaggca cgtatatcgt tctgcccgcg aagccatttg gcccgtatga
    10621 agagggcggg aacgaagaag agtagcttat ggagaggggc ggtgctgcct ctctcattca
    10681 ttccggt
//
LOCUS       DAJVXP010000136        10678 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVXP010000136 DAJVXP010000000
VERSION     DAJVXP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.136"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1187)
                     /gene="aroA"
                     /locus_tag="OQS10_003376"
     CDS             complement(<1..1187)
                     /gene="aroA"
                     /locus_tag="OQS10_003376"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS2294323.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVA"
     gene            complement(1258..2346)
                     /gene="serC"
                     /locus_tag="OQS10_003377"
     CDS             complement(1258..2346)
                     /gene="serC"
                     /locus_tag="OQS10_003377"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS2294324.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2545..3237)
                     /locus_tag="OQS10_003378"
     CDS             complement(2545..3237)
                     /locus_tag="OQS10_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCS2294325.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3367..5127
                     /locus_tag="OQS10_003379"
     CDS             3367..5127
                     /locus_tag="OQS10_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS2294326.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5533..6390
                     /gene="focA"
                     /locus_tag="OQS10_003380"
     CDS             5533..6390
                     /gene="focA"
                     /locus_tag="OQS10_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS2294327.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6445..8727
                     /gene="pflB"
                     /locus_tag="OQS10_003381"
     CDS             6445..8727
                     /gene="pflB"
                     /locus_tag="OQS10_003381"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS2294328.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            8919..9659
                     /gene="pflA"
                     /locus_tag="OQS10_003382"
     CDS             8919..9659
                     /gene="pflA"
                     /locus_tag="OQS10_003382"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS2294329.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9752..9928)
                     /locus_tag="OQS10_003383"
     CDS             complement(9752..9928)
                     /locus_tag="OQS10_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCS2294330.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10028..>10678
                     /locus_tag="OQS10_003384"
     CDS             10028..>10678
                     /locus_tag="OQS10_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2294331.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGN"
BASE COUNT         2751 a         2678 c         2531 g         2718 t
ORIGIN      
        1 aacgccacca gcgagaagca catcgccatc cggtgatcat tgtatgtcgc gatctcggca
       61 aagttcagtt tttccggtgg agtgatacga atgtaatcgt gcccctcttc tacttccgca
      121 ccgactttac gcagttctgt tgccatcgca aacaggcgat cggtctcttt aacacgccag
      181 ttatagatat tgcgcagcgt ggtggtgcct tttgcaaata acgccgccgt ggcaatggtc
      241 atcgccgcat cgggaatatg gttcatatcc atatcaatag cgttcagttc accacgcgtg
      301 caggaaatat aatcatcgcc ccagcaaatg gtcgcgccca ttttttccag cacatcagca
      361 aagcgaatat caccctgcat actgttacgt ccaataccgg tcacttttac agtgccgcct
      421 ttgattgctg ctgctgccag aaagtaagaa gccgaagatg catcgccttc gaccaaataa
      481 gtacccggag actgataaga ctgcccgcct tttacgacaa attgttgata gtgctgattt
      541 tcaatttcaa caccaaacgt cttcatcaga ttgagtgtga tgtcgatata aggtttagaa
      601 accagatcgc ctttaatacg aatcaccgta tcttccggcg caagaggcgc agtcattaac
      661 agtgcggtga ggaattggct ggaaacggag ccatcaacgt caacgttgcc gccagtaaag
      721 ccgccctgta aacgcaacgg cggataattt tcttgttcca ggtaagtgat cttcgccccg
      781 cccaggcgca gcgcatccac cagatgacca atcgggcgtt ctttcatacg cggctcaccg
      841 gtcagcacaa tatcattgct acccagacaa agagctgccg ccagcggacg cattgccgtt
      901 ccggcgttac cgaggaacaa ctccagggca ccttctgcgt gtaatggacc gccgttaccg
      961 ataatttcgc aacgcgtacg atcggctgaa agcgtatagc ttacccctaa cgctgttaat
     1021 gcattcagca tatggcgcac gtcatcgcta tccagcagat tggttaatac tgttttgccg
     1081 tgtgctaatg ccgccagcaa taaagcgcgg ttagaaacgc tcttggaacc gggcagatta
     1141 atagtgccat cgacacgagc gatgggttgt aacgtcaggg attccatgaa ctcaactctc
     1201 tacaacagaa ataaaaaccc cacaggctgg ctgtggggat taagcaaaat ttcggcatta
     1261 accgtgacgg cgttcgaact caaccatgaa gtctgtcagc gctttaacgc cttccagcgg
     1321 catggcgtta taaatagaag cgcgcattcc gccgaccaca cggtgacctt tcagtgcatg
     1381 aaggccagca gcaaaagact cttcaaggaa caatttgtca agcgcactgt ccgccaactg
     1441 gaacggcacg ttcatccgcg aacggttagc tttcgccacg tcattgcggt agaaatcgct
     1501 gttatcaatc accccatata gcagttctgc tttttgctga ttgattttat ccatttcagc
     1561 tacaccgccg ttcgctttca gccatttaaa gaccagacca gatagatacc aggcaaatgt
     1621 cggcggcgtg ttaaacatgg agccgttatc gttgaggatg gaataatcca gaatcgacgg
     1681 acacgcgata ttcgctttgc ccagcaaatc ttcacgaacg atgacgattg tcaggccagc
     1741 cgggccgata tttttctgcg cgccagcgta aatcacgcca taacggctga cgtcaatcgg
     1801 acgggaaaga atggttgaag agaagtcggc ggcgaccacc acatctttgc cgaagtctgg
     1861 cgtttcgtcg atggcgatac cgtcgatggt ttcattcggg cagtaatgca tataagcagc
     1921 attatcagag agttgccatt cacgcattgg cttaaccgcg cgcagaccat caacagtcac
     1981 tttggcgtca aagacattag gcgtgcagta ttttttcgct tctttaatgg cacttgccgc
     2041 ccagtaaccg gcatcaacat aatctgcggt ggttttatca ccgagaatat tcagcggtac
     2101 cgcagcaaac tgaccgcgac caccgccatg gcagaataat accttgtagt tggaggggac
     2161 attaagaaga tcgcgaaaat ccttctcggc ttcctctgca acctgaatga actctttgcc
     2221 acggtgactc acttccatca ccgacgtacc aagaccgttc cagtcgcgca gttcctgttg
     2281 agcctgttta agcacctctg ccggtagcat tgccggacca gaactaaaat tgaagatttg
     2341 agccattgcc cctcaccacg ttgcgttgct attaacccgc ggtcaatcga ttgcgaccac
     2401 aggtaataca ttattcctgt ggctatcggt tttatcattc agtgacacac atcgcaatga
     2461 gtaaaaccgt tgagggcaaa aatgtggctt gcatcacaca aaagaatctc tcggattatt
     2521 agcaaaaagc cgatgttttt actgtcaggt cacccgtttt gcccgacttg ccttcgtcca
     2581 ttctggattt gcacagcgcg gacataattg tttttccccc gctttcatat gaatgatttc
     2641 actacaacga atacaggcat agtccgccga ctccggcacc actttgtaac gctgcgtaca
     2701 atcactgggt aaaataagta aacccggttt caccttgtca tcttcaaaga aatagacact
     2761 gatttgcccg ttggtttcga gaatcgccag acgtacctgc cccagctgct ccacgccacg
     2821 caatcgtagc tccataaaga actcaaattc cgtcatgttg gagttattga gtttcgacca
     2881 ggccagctcg ccatcttcaa taataacaac cggcttacct tccagtagat cttccagttt
     2941 ttcactgtgc gccatcaacc acattaccag gcggtataac aacgccagag tgataaagac
     3001 gataagtacc ggcaccatcg gcacatcatc ataaaacgcc acatctcccg ccgctgaacc
     3061 cagcgtgaga atgattaaca cttcaaacag cgacatctgt cgcacaccgc gtcttccggt
     3121 cattttgagg aacaaaaaaa ccagtacaaa ggtataaaga ctacgtagtg caacttcgcc
     3181 aaggaaatca aaaggcactt tatcaaatgc catacggtgg agatcgaacg ccttcatttt
     3241 tatgctctta tcgtggttta tccgctaaaa gcatagtcaa cagttcatcg gcatggcggc
     3301 ggtgatagcg cctttgagat aaaacccgta ttattgcgcg ctttccgtac gactaaagtg
     3361 attttcatga cgcaaacatt tatccccggc aaagatgccg ctctggaaga ttccatcgct
     3421 cgcttccagc aaaaactttc agacctcggc tttcagattg aagaggcctc ctggctgaat
     3481 cctgtgccta acgtctggtc tgtacatatt cgcgacaaag agtgcgcact gtgttttacc
     3541 aacggtaaag gcgcaaccaa aaaagcggcg ctggcttctg cacttggtga atatttcgag
     3601 cgtctctcaa ccaactactt ttttgctgat ttctggctgg gcgaaaccat cgccaacggt
     3661 ccgttcgtgc attatcccaa cgaaaaatgg ttcccactga ccgaaaatga cgatgtgcca
     3721 gaaggactac tcgatgaccg tctgcgcgcg ttttatgatc cggagaatga actgaccggc
     3781 agcatgctga ttgacctaca atccggtaac gaagatcgtg gtatttgcgg cctgccgttt
     3841 acgcgtcagt ccgacaatca gaccgtttat attccgatga atatcattgg taacctgtac
     3901 gtctccaacg gtatgtccgc aggtaatacc cgcaacgaag cacgcgttca gggattgtct
     3961 gaagttttcg aacgctacgt gaaaaaccgc attattgctg aaagcatcag cctgccagag
     4021 atcccggcag acgtgctggc gcgttaccca gcagtagttg aagccatcga aacgctggaa
     4081 gcggagggtt tcccaatctt cgcttatgac ggttcgcttg gcggccagta tccggtgatt
     4141 tgcgtggtac tgttcaatcc tgctaacggt acctgctttg cctctttcgg tgcgcatcct
     4201 gattttggcg tagcactgga acgtaccgtg accgagctgc tgcaaggtcg tggcctgaaa
     4261 gatttggatg tgtttactcc gccaaccttc gatgatgaag aagtcgctga acataccaac
     4321 ctcgaaacgc actttatcga ttccagcggt ttaatctcct gggacctgtt caagcaggat
     4381 gccgattatc cgtttgtgga ctggaatttc tccggcacca cggaagaaga gtttgctacg
     4441 ctgatggcta tcttcaacaa agaagataaa gaagtttata ttgccgatta cgagcatctg
     4501 ggcgtttatg cttgccgtat tatcgtgcct ggcatgtccg atatttatcc ggctgaagat
     4561 ctgtggctcg cgaataacag tatgggcagc catttacgtg aaacgattct ttcgctacca
     4621 ggcagcgagt gggaaaaaga agattacctg aacctcatcg agcaactgga tgaagaaggt
     4681 tttgatgact ttacccgcgt gcgtgagctg ttgggtctgg cgaccgggtc ggataacggt
     4741 tggtacaccc tgcgtatcgg tgaattaaaa gccatgctgg cgctggctgg tggcgatctg
     4801 gaacaggctc tggtctggac cgaatggacg atggagttta actcatcagt atttagtccg
     4861 gaacgcgcca actattatcg ctgcctgcaa acgttgttat tactggcaca ggaagaagat
     4921 cgccagccgc tgcaatatct gaatgcgttt gttcgcatgt acggcgcaga tgccgtagaa
     4981 gccgccagtg cggcaatgag cggcgaagcg gcgttttacg gcctgcaacc ggtagatagc
     5041 gatctgcacg cgtttgctgc acatcagtcg ttgttgaagg cctacgaaaa gctgcagcgc
     5101 gccaaagcag cattctgggc aaaataaaat caaatagcct acgcaatgta ggcttaatga
     5161 ttagtctgag ttatattacg gggcgttttt ttaatgcccc gctttacata tatttgcatt
     5221 aataaaataa ttgtaattat aaggttaaat atcggtaatt tgtatttaat aaatacgatc
     5281 gatattgtta ctttattcgc ctgatgctcc cttttaatta actgttttag cggaggatgc
     5341 ggaaaaaatt caactcattt gttaattttt aaaatttatt tttatttgga taatcaaatg
     5401 cttactccgt atctgcataa aaaccatgcg agttacgggc ctataagcca ggcgagatat
     5461 gatctatatc aatttctcat ctataatgct ttgttagtat ctcgtcgccg acttaataaa
     5521 gagagagtta gtgtgaaagc tgacaaccct tttgatcttt tacttcctgc tgcaatggcc
     5581 aaagtggccg aagaggcggg tgtctataaa gcaacgaaac atccgcttaa gactttctat
     5641 ctggcgatta ccgccggtgt tttcatctca atcgcattcg tcttctatat cacagcaacc
     5701 actggcacag gcacaatgcc cttcggcatg gcaaaactgg ttggcggcat ttgcttctct
     5761 ctggggctga ttctttgtgt tgtctgcgga gccgatctct ttacttccac cgtgttgatt
     5821 gttgttgcta aggcgagtgg gcgcatcacc tggggtcagt tggcgaaaaa ctggctaaat
     5881 gtctattttg gcaacctggt cggcgcactg ctgtttgtac ttttaatgtg gctttccggc
     5941 gagtatatga ccgcaaatgg tcaatgggga ctaaacgtcc tacaaaccgc cgaccacaaa
     6001 gtgcaccata cttttattga ggccgtctgt cttggtatcc tggcaaacct gatggtatgt
     6061 ctggcagtat ggatgagtta ttctggccgc agcctgatgg acaaagcgtt cattatggtg
     6121 ctgccggtcg cgatgtttgt tgccagcggt tttgagcaca gtatcgcaaa catgtttatg
     6181 atcccgatgg gtattgtaat ccgcgacttc gcatccccgg aattttggac cgcagtcggt
     6241 tctgcaccgg aaaatttttc tcacctgacc gtgatgaatt tcatcactga taacctgatt
     6301 ccggttacga tcggcaacat tatcggtggt ggtttgttgg ttgggttgac atactgggtc
     6361 atttacctgc gtgaaaacga ccaccattaa tggttgtcga agtacgcagt aaataaaaaa
     6421 tccacttaag aaggtaggtg ttacatgtcc gagcttaatg aaaagttagc cacagcctgg
     6481 gaaggtttta ccaaaggtga ctggcagaat gaagtaaacg tccgtgactt cattcagaaa
     6541 aactacactc cgtacgaggg tgacgagtcc ttcctggctg gcgctactga agcgaccacc
     6601 accctgtggg acaaagtaat ggaaggcgtt aaactggaaa accgcactca cgcgccagtt
     6661 gactttgaca ccgctgttgc ttccaccatc acctctcacg acgctggcta catcaacaag
     6721 cagcttgaga aaatcgttgg tctgcagact gaagctccgc tgaaacgtgc tcttatcccg
     6781 ttcggtggta tcaaaatgat cgaaggttcc tgcaaagcgt acaaccgcga actggatccg
     6841 atgatcaaaa aaatcttcac tgaataccgt aaaactcaca accagggcgt gttcgacgtt
     6901 tacactccgg acatcctgcg ttgccgtaaa tctggtgttc tgaccggtct gccagatgca
     6961 tatggccgtg gccgtatcat cggtgactac cgtcgcgttg cgctgtacgg tatcgactac
     7021 ctgatgaaag acaaactggc acagttcact tctctgcagg ctgatctgga aaacggcgta
     7081 aacttggaac agactatccg tctgcgcgaa gaaatcgctg aacagcaccg cgctctgggt
     7141 cagatgaaag aaatggctgc gaaatacggc tacgacatct ctggtccggc taccaacgct
     7201 caggaagcta tccagtggac ttacttcggc tacctggctg ctgttaagtc tcagaacggt
     7261 gctgcaatgt ccttcggtcg tacctccacc ttcctggatg tgtacatcga acgtgacctg
     7321 aaagctggca agatcaccga acaagaagcg caggaaatgg ttgaccacct ggtcatgaaa
     7381 ctgcgtatgg ttcgcttcct gcgtactccg gaatacgatg aactgttctc tggcgacccg
     7441 atctgggcaa ccgaatctat cggtggtatg ggcctcgacg gtcgtaccct ggttaccaaa
     7501 aacagcttcc gtttcctgaa caccctgtac accatgggtc cgtctccgga accgaacatg
     7561 accattctgt ggtctgaaaa actgccgctg aacttcaaga aattcgccgc taaagtgtcc
     7621 atcgacacct cttctctgca gtatgagaac gatgacctga tgcgtccgga cttcaacaac
     7681 gatgactacg ctattgcttg ctgcgtaagc ccgatgatcg ttggtaaaca aatgcagttc
     7741 ttcggtgcgc gtgcaaacct ggcgaaaacc atgctgtacg caatcaacgg cggcgttgac
     7801 gaaaaactga aaatgcaggt tggtccgaag tctgaaccga tcaaaggcga tgtcctgaac
     7861 tatgatgaag tgatggagcg catggatcac ttcatggact ggctggctaa acagtacatc
     7921 actgcactaa acatcatcca ctacatgcac gacaagtaca gctacgaagc ctctctgatg
     7981 gcgctgcacg accgtgacgt tatccgcacc atggcgtgtg gtatcgctgg tctgtccgtt
     8041 gctgctgact ccctgtctgc aatcaaatat gcgaaagtta aaccgattcg tgacgaagac
     8101 ggtctggcta tcgacttcga aatcgaaggc gaatacccgc agtttggtaa caacgatccg
     8161 cgtgtagatg acctggctgt tgacctggta gaacgtttca tgaagaaaat tcagaaactg
     8221 cacacctacc gtgacgctat cccgactcag tctgttctga ccatcacttc taacgttgtg
     8281 tatggtaaga aaacgggtaa caccccagac ggtcgtcgtg ctggcgcgcc gttcggaccg
     8341 ggtgctaacc cgatgcacgg tcgtgaccag aaaggtgcag tagcctctct gacttccgtt
     8401 gctaaactgc cgtttgctta cgctaaagat ggtatctcct acaccttctc tatcgttccg
     8461 aacgcactgg gtaaagacga cgaagttcgt aagaccaacc tggctggtct gatggatggt
     8521 tacttccacc acgaagcatc catcgaaggt ggtcagcacc tgaacgttaa cgtgatgaac
     8581 cgtgaaatgc tgctcgacgc gatggaaaac ccggaaaaat atccgcagct gaccatccgt
     8641 gtatctggct acgcagtacg tttcaactcg ctgactaaag aacagcagca ggacgttatt
     8701 actcgtacct tcactcaatc tatgtaatta gatttgactg aaatcgtaca gtaaaaagcg
     8761 tacaataaag gctccacgaa agtggggcct tttttagcac gagagccttt tttgtcagct
     8821 atctatactc taaggtgact gccaaaacag actcgacgta gccttcgagc tgcgcaccaa
     8881 cacggcctca gatgggccac atctggagaa acaccgcaat gtcagttatt ggtcgcattc
     8941 actcctttga atcctgtgga accgtagacg gcccgggtat tcgctttatc acctttttcc
     9001 agggctgcct gatgcgctgc ctgtattgtc ataaccgcga cacctgggat acgcatggcg
     9061 gtaaagaagt taccgttgaa gatttaatga aagaggtggt gacctatcgc cactttatga
     9121 acgcttccgg cggcggcgtt accgcatccg gcggtgaggc aatcctacaa gctgagtttg
     9181 ttcgtgactg gttccgcgcc tgcaaaaaag aaggcattca tacctgtctg gacaccaacg
     9241 gttttgttcg tcgttacgat ccggtgattg atgaactgct ggaagtaacc gacctggtaa
     9301 tgctcgatct caaacagatg aacgacgaga tccaccaaaa tctggttgga gtttccaatc
     9361 accgcacgct ggagttcgcc aaatatctgg cgaacaaaaa tgtgaaggtg tggatccgct
     9421 acgttgttgt cccaggctgg tctgacgatg acgattcagc gcatcgcctc ggtgaattta
     9481 cccgtgatat gggcaacgtt gagaaaatcg agcttctccc ctaccacgag ctgggcaaac
     9541 acaaatgggt ggcaatgggt gaagagtaca aactcgacgg tgttaaacca ccgaagaaag
     9601 agaccatgga acgcgtgaaa ggcattcttg agcagtacgg tcataaggta atgttctaat
     9661 ttccccgtaa agcggcaact ttattgagtt gccgcttttt tattccgcac ttcattctgc
     9721 tacagcgttt ttaacacatc ttatagtgca tctaccatat cccgtacctg agataataac
     9781 gactgataat attacgccga tataaatcga gttccgtcac ctgtattttc cgctccattg
     9841 cccgctgatg gatcgcatca gcaatatgtg cggtcaatga atcatgacga ggatgggccc
     9901 ataccaaata aatacgttca gactgcataa ttttacctcc acgttaagtt ctggagcgca
     9961 ctttacgcag gcggcataat aatgtaaacg tgatgattca ggatgagtta atgaaggatt
    10021 ttcactaatg cggatgaata tgtctgactt tgccactttc tttgctgtgg cccgtaatca
    10081 aagctttcgt gcagcgggcg atgagttagg cttatcctcg tccgccatta gccatagtat
    10141 taaaacactg gaacaacgtc ttaaaattcg cttattcaat cggacaaccc gaagcgtatc
    10201 gttaacggaa gcgggttcta atctctatga acgattgcgc ccagctttcg acgaaattca
    10261 gatcatgtta gatgagatga atgattttcg tctgacacca acaggaacgc tcaaaatcaa
    10321 tgccgcccgg gtagctgcac gtatattttt aatgccttta ctggttggtt tcacacgtga
    10381 atatcctgat attaaagtcg agctcacaac tgacgactct cttgtcgata tcgtccaaca
    10441 gggctttgac gctggcgttc gacttagtgg catcgtcgaa aaagatatga tcagcgtggc
    10501 gatcggccca ccagtgaaat tatgtgtagc agcaacgcca gagtactttg caagatatgg
    10561 caaaccccgc catcctcatg atctgcttaa tcatcaatgt gttgtatttc gttatcccag
    10621 cggcaaacct ttccactggc aatttgcaaa ggagcaggaa attgctgtcg cagggaat
//
LOCUS       DAJVXP010000137        10553 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVXP010000137 DAJVXP010000000
VERSION     DAJVXP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.137"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..613
                     /locus_tag="OQS10_003385"
                     /pseudo
     CDS             1..613
                     /locus_tag="OQS10_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            610..1224
                     /locus_tag="OQS10_003386"
     CDS             610..1224
                     /locus_tag="OQS10_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS2294332.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OQS10_003387"
     CDS             complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OQS10_003387"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS2294333.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2999..3457
                     /gene="gpt"
                     /locus_tag="OQS10_003388"
     CDS             2999..3457
                     /gene="gpt"
                     /locus_tag="OQS10_003388"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS2294334.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3549..4793
                     /gene="frsA"
                     /locus_tag="OQS10_003389"
     CDS             3549..4793
                     /gene="frsA"
                     /locus_tag="OQS10_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS2294335.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4851..5252
                     /gene="crl"
                     /locus_tag="OQS10_003390"
     CDS             4851..5252
                     /gene="crl"
                     /locus_tag="OQS10_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS2294336.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="OQS10_003391"
     CDS             complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="OQS10_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS2294337.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6634..7737
                     /gene="proB"
                     /locus_tag="OQS10_003392"
     CDS             6634..7737
                     /gene="proB"
                     /locus_tag="OQS10_003392"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS2294338.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7749..9002
                     /gene="proA"
                     /locus_tag="OQS10_003393"
     CDS             7749..9002
                     /gene="proA"
                     /locus_tag="OQS10_003393"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS2294339.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9117..9192
                     /locus_tag="OQS10_003394"
     tRNA            9117..9192
                     /locus_tag="OQS10_003394"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Thr,seq:cgt)
     gene            9798..9956
                     /locus_tag="OQS10_003395"
     CDS             9798..9956
                     /locus_tag="OQS10_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294340.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2653 a         2532 c         2700 g         2668 t
ORIGIN      
        1 gtctatttta cagcggcata ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga
       61 cgacgcgcta cggtatattg cggaacatga cgatgcgctg gcgtttgcgc gtattaatcg
      121 ccagctgatc tctttacgca taatgcaaca ggttaaggcc accggaagtc cggttctgga
      181 tgtggcgcat aactttgtta gcgcgtgtca aatcggcgat caacagggct ggttgcatcg
      241 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac tcggtgatta
      301 ctcctagctt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
      361 tgggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat acacagcgac
      421 gcagctctca cgtactgaac ttggcagtcg ggtaatttgt cgcgataaac aactcatctt
      481 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
      541 tggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
      601 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
      661 gcagtgaaaa aggcgctgga gcgattaatt aaagaagctg cccgacaaga cgtcgcggta
      721 acggtgttgg aaacagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
      781 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gcggcactat tcagtggatt
      841 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
      901 tttaccgctg atgagcagga acaatcggat gcaatccgtt atgagacgtt gcgttcgtcg
      961 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
     1021 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
     1081 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
     1141 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
     1201 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
     1261 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
     1321 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
     1381 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
     1441 tccaggcccg cgtggataat ctggatgttc ggcgttttgt tgaacagacg ctgataggtt
     1501 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
     1561 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
     1621 tctttaccgc tgtcgatcag agaacggatc agacagtgaa tttctacgtt attatcagtc
     1681 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtctgagttg
     1741 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
     1801 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
     1861 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt caggacgtcg
     1921 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
     1981 gtgccgccgt tgaaatcgat aaggcgcaga tccagttctt ctgcatgacc cgctaggaag
     2041 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttccccgcc ggagtgaccc
     2101 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
     2161 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
     2221 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
     2281 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
     2341 accacgtttt cgtcagccag aaccgccagc gcagacgcca taccaatgcc gttatctgca
     2401 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
     2461 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
     2521 aagacgaccg atttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
     2581 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
     2641 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
     2701 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
     2761 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
     2821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     2881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     2941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3121 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3181 cagctacgat cacgacaacc agcgcgagct taaagtgcta aaacgcgcag aaggcgatgg
     3241 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     3301 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3361 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     3421 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3481 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     3541 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3601 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3661 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3721 ggcgcggcat tgacccacgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg
     3781 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggt ggcaactgga
     3841 tttatgagtg ggccatccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
     3901 cgcaactcag tggtcgtcac tggctgcatg cggctacgtt gtacaacatt gctgcctatc
     3961 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgtgcctatg
     4021 aagaagccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4081 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4141 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4201 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcatcaa
     4261 aatggaagct cacccaggac tccagcctgt tgcatcagca cgttttaaag gctctgccta
     4321 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4381 ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4441 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4501 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4561 agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa
     4621 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4681 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4741 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4801 ctaaaatttg ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac
     4861 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     4921 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     4981 tgaaaccggc accggaagtg cgtgagttct ggggctggtg gatggagctt gaagcgcagg
     5041 aatcccgttt tacctacagt taccagtttg gtctgttcga taaagcaggc gactggaaga
     5101 gtgttccggt aaaagacact gaagtggttg aacgactgga gcacacccta cgtgagtttc
     5161 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt
     5221 ttcgtgatga accggtgaag ttaacggcgt gagtgaaatg tgccggatgt atcacatccg
     5281 gcaatatcca ttaaaactga taggtcatgc caaccgcgac aatatcatca ttattaatat
     5341 tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaacgct gacatatttt
     5401 tgttgaaata atacgtagca ccgacgtcga tataattgac cagatcttca tcaccgatac
     5461 cttcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5521 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5581 gcgtcatttt gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5641 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5701 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5761 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     5821 ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt
     5881 tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg
     5941 aatcgccacc aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcgccca
     6001 ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac
     6061 gcgttttctg ctgtgcagta tcactctccg ctttatttcc ggcaaactcc gcttcccacc
     6121 gaccataacc agtcagttga tcgttaattt gtgtttcacc tttaaaacca aaacggatat
     6181 aactctggtc gccatcttta ctggcgttat cactcatata atgcatagct ttaactttgc
     6241 catagacatc cagtttatta ccgtctttat tatatatttc tgcagcctgt acagatgcag
     6301 atgccacaat gcccatcacc actaatgcca gagtgctctt tttcattttc attcctgatt
     6361 ttaattaacg cgcgaatatt cagcgggaga gccccgttga aaacaggaaa gtttttaacc
     6421 tgagattgtt aaagatatat tacagattaa taatattctt aaaatgtggt aatttattaa
     6481 atctgtaata aaagcgtaaa caactgccgc taggcttgct gatcccgcgc aacaaaacgc
     6541 catgctttgc tcgcagatgg ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat
     6601 atcatttttc ctaaaattga atggcagaga atcatgagtg acagccagac gctggtggta
     6661 aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc tgaaccgtgc ccatattgtt
     6721 gaacttgttc gccagtgcgc gcagttacat gccgccgggc atcggattgt tattgtgacg
     6781 tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc cggaactgcc agcgactatc
     6841 gcctcgaaac aactgctggc agcggtaggg cagagtcgac tgattcaact gtgggaacag
     6901 ctgttttcga tttatggcat tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa
     6961 gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg cgttgctcga taacaatatc
     7021 gttccggtaa tcaatgagaa cgatgctgtc gctacggcag agattaaagt cggcgataac
     7081 gataaccttt ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt gctgctgacc
     7141 gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga actgattaaa
     7201 gatgtttacg gcattgatga cgcactgcgc gcgattgccg gtaacagcgt ttcaggcctc
     7261 ggaactggcg gcatgagtac caaattgcag gccgctgacg tggcttgccg tgcgggtatc
     7321 gacaccatta ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt
     7381 tccgtcggta cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa acgctggatt
     7441 ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag gggcaactgc cgccattctg
     7501 gaacgcggca gctccctgtt gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt
     7561 ggtgaagtca tccgcatttg caacctcgaa ggccgcgata tcgcccacgg cgtcagtcgt
     7621 tacaacagcg atgcattacg ccgtattgcc ggacaccact cgcaagaaat tgatgcaata
     7681 ctgggatatg aatacggccc ggttgccgtt caccgtgatg acatgattac ccgttaagga
     7741 gctggccgat gctggaacaa atgggcattg ctgcgaagca agcctcgtat aaattagcgc
     7801 aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac
     7861 aaagcgaaat catcctcaac gctaacgccc aggatgttgc tgacgcgcga gccaatggcc
     7921 ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc acggctgaaa ggcattgccg
     7981 acgatgtacg tcaggtgtgc aacctcgccg atccggtggg gcaggtaatc gatggcggcg
     8041 tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc gctgggggtt attggcgtga
     8101 tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta
     8161 atgcggtgat cctgcgcggt ggcaaagaaa cgtgtcgcac taacgctgca acggtggcgg
     8221 tgattcagga cgccctgaaa tcctgcggct taccggcggg tgccgtgcag gcgattgata
     8281 atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga taaatacatc gacatgctga
     8341 tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga acagtcgaca atcccggtga
     8401 tcacaggtgg tataggcgta tgccatattt acgttgatga aagtgtagag atcgctgaag
     8461 cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag cacatgtaat acggttgaaa
     8521 cgttgctggt gaataaaaac atcgccgata gcttcctgcc cgcattaagc aaacaaatgg
     8581 cggaaagcgg cgtgacatta cacgcagatg cagctgcact ggcgcagttg caggcaggcc
     8641 ctgcgaaggt ggttgctgtt aaagccgaag agtatgacga tgagtttctg tcattagatt
     8701 tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc ccatattcgt gaacacggca
     8761 cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa cgcccagcgt tttgttaacg
     8821 aagtggattc gtccgctgtt tacgttaacg cctctacgcg ttttaccgac ggcggccagt
     8881 ttggtctggg tgcggaagtg gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg
     8941 ggctggaagc actgaccact tacaagtgga tcggcattgg tgattacacc attcgtgcgt
     9001 aaataaaacc gggtgatgca aaagtagcca tttgattcat aaggccattg acgcatcgcc
     9061 cggttagttt taaccttgtc caccgtgatt cacgttcgtg aacatgtcct ttcagggccg
     9121 atatagctca gttggtagag cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta
     9181 ttatcggcac cattaagaaa attaataact tagcttaact tctctaagaa attgtatctt
     9241 ctgttatcac ttacaaacgc ctatacgttg ttatgggctt tggtagtgta acacgtatgg
     9301 gcgagtagtt cccaggctca acctcgtaac aagttccttt gaagctggaa gacgtgccac
     9361 cgtctctaaa ccttctacca aaactcttag caagatgttc cacacctggc gcggtgtcca
     9421 atgatgctac acgtcgtgag acgttgcaga ggataaacag gctaaatgat gggaagagtt
     9481 ttaagtgcaa tagtggatta aagcccacct cttcaaaacc taacttgatc gggaaagaat
     9541 caagctctag cacggtttcg tccaccggag tacaacgggt gcctgataag agaagaactg
     9601 ctaagacttc atagcttttg tgaaaaagcc ctttttagtg gatacgccta cggcgcttat
     9661 tttttggtat aaccatcaaa ggcgctttat ggctcctatt gtcttgaaaa tgtatcttta
     9721 cttgtattgt gttttatgcg tagtctccct gtgtagtatg taacttgtta attttcatat
     9781 gaatacagag gcacacgatg aaaaacattg cagcaatcaa acgtaataac cgcaagattc
     9841 acgctcgtaa gttcctgtct acgccagaag gaaaagcctg gctagagcgt aaacagagag
     9901 agaacgaaga aagaaaactc cttagtgagt tgaaatggct tagagaggat ttctgaaaat
     9961 ctccagaacg caacaggacg cattctaacg ctgttttcat tactcggtat agggataggt
    10021 gatgtgtgtt taatgcgtct gtgcgcttgt tttgtgcctt attttgcatt ttcataatgc
    10081 ggaagagaac acggaaaacc ttaaaattaa ttattggggt atttaaggct attttcaggt
    10141 gatttaaggg tattaaactt gatttaattg attaaaatat aaacagataa ttatgtatct
    10201 tattgattta taaggttgtt tttaagtaaa ggtaaataaa aaataaatag ccataaatag
    10261 ctataaatac atcttaatac ctatatccct tcccttatgg ctagattgcg agaggtgctt
    10321 aacgcgattt tcagcgcccc ccgatctgga aaatccagaa tccccaaata tcagcaagga
    10381 attttcttgc gtaatcaaca tcgtttttac ctattaaatc attacacaga aagcctttct
    10441 gtaatgtctc tcactaaact ttcactaaac acgagaatga tcggttttat gtcggatcag
    10501 atgctagaaa cagcaccacg tctcacaagg gctgtaagcg atgaaaccag tgt
//
LOCUS       DAJVXP010000138        10486 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVXP010000138 DAJVXP010000000
VERSION     DAJVXP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.138"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..2270)
                     /gene="gyrB"
                     /locus_tag="OQS10_003396"
     CDS             complement(<1..2270)
                     /gene="gyrB"
                     /locus_tag="OQS10_003396"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS2294341.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDP"
     gene            complement(2299..3372)
                     /gene="recF"
                     /locus_tag="OQS10_003397"
     CDS             complement(2299..3372)
                     /gene="recF"
                     /locus_tag="OQS10_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS2294342.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(3372..4472)
                     /gene="dnaN"
                     /locus_tag="OQS10_003398"
     CDS             complement(3372..4472)
                     /gene="dnaN"
                     /locus_tag="OQS10_003398"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS2294343.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(4477..5880)
                     /gene="dnaA"
                     /locus_tag="OQS10_003399"
     CDS             complement(4477..5880)
                     /gene="dnaA"
                     /locus_tag="OQS10_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS2294344.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6177..6257
                     /locus_tag="OQS10_003400"
     CDS             6177..6257
                     /locus_tag="OQS10_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294345.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            6487..6627
                     /gene="rpmH"
                     /locus_tag="OQS10_003401"
     CDS             6487..6627
                     /gene="rpmH"
                     /locus_tag="OQS10_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS2294346.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            6644..7003
                     /gene="rnpA"
                     /locus_tag="OQS10_003402"
     CDS             6644..7003
                     /gene="rnpA"
                     /locus_tag="OQS10_003402"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS2294347.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            6967..7224
                     /gene="yidD"
                     /locus_tag="OQS10_003403"
     CDS             6967..7224
                     /gene="yidD"
                     /locus_tag="OQS10_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS2294348.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            7227..8873
                     /gene="yidC"
                     /locus_tag="OQS10_003404"
     CDS             7227..8873
                     /gene="yidC"
                     /locus_tag="OQS10_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS2294349.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            8979..10343
                     /gene="mnmE"
                     /locus_tag="OQS10_003405"
     CDS             8979..10343
                     /gene="mnmE"
                     /locus_tag="OQS10_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS2294350.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         2344 a         2897 c         2755 g         2490 t
ORIGIN      
        1 gggtccatgg tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc
       61 tggatggaga ggccgcgacg ggactctttc accagccagt ccagcgcctg ctcgaagctg
      121 gctaccggct gacgacgctc gccacgttcg ataaacgcat cttcttccag caagccacgc
      181 agtttctcac ccagcgtgca gatacgacga tattcgccac cggtgataaa ctcgtgatcc
      241 agcggatagt cagtatccac accgtgggta cgcacgcgaa caatcggttc aaacaggttt
      301 tgttcggcat tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc
      361 agttcgctga ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct
      421 tccgtcagcg tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc
      481 atgcggttga tcattttctg cgtcgcgttg tactcagaca ccaatttctc taacgcttcg
      541 ccagccaatg ccggtgcgct ggcgttggtg tgcagcgttg cgccatccag cgcgatagag
      601 atctggtact gatccatcgc ttcgtcgtct ttaatgtact gttcttgctt gcctttcttc
      661 actttgtaca gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc
      721 tgacgataga aaaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg
      781 gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata
      841 ccacagccaa gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag
      901 cgcgctttct caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg
      961 ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa
     1021 agcgccggat cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc
     1081 gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca
     1141 tcgataattt tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc
     1201 agcagttcgt tcatctgctg ttcaaccgct gatttcacct cagaagaaac cagtttgtct
     1261 ttggtctggg aggagaattt cgggtccggc actttcacgg aaacgaccgc aatcaggcct
     1321 tcacgcgcat cgtcaccggt ggcgctaact ttggcttttt tgctgtagcc ttctttgtcc
     1381 atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg
     1441 tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac
     1501 tgcaacgcca cttcgacgcc aataccgtct ttttcagtgg agaagtagaa gatattcggg
     1561 tggatcggcg ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag
     1621 tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac gaatggaaac gccggagttg
     1681 aggaacgaca actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg
     1741 gtgaaggttt cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg
     1801 gtaaccgcca acggtgcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc
     1861 tcgcgctgga taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg
     1921 ccgtgcagac cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga
     1981 acggtcatga tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg
     2041 ccgcgcccgt catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct
     2101 ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg
     2161 tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca
     2221 tccagccctt tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct
     2281 cgctcattta tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga
     2341 attttcgtcc gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga
     2401 ttgcgtcgct tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc
     2461 aaaatcatct atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg
     2521 cgccagacgt aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac
     2581 cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt
     2641 tagctggcga tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc
     2701 ccagccgcgc tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc
     2761 ggccatatca gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccgc
     2821 cagcgggatc agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag
     2881 cgccgcattg cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg
     2941 ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg tatttggggc cgccgttgag
     3001 taaagtaaac ccttctggcg ttatcaactg cattggcatc aggtgcgcca gttccgcgac
     3061 cttatgcccg tctgtaccgt cgatgcggac tttgctgtcg ccctgtttgt ctttggttaa
     3121 gccaatcgct gtctcgcgct cttcgccctg taatcgcccg tggagaacaa acgcctcctg
     3181 ctcatggcga atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt
     3241 atagatggct tccagcacgc tggttttgcc actgccgttg gcacctacca gaaagttaaa
     3301 gccgggagat aaagcgagat ccgcggtttc aatgttgcgg aaatcgcgga tcaacaagcg
     3361 ggtgagggac attacagtct cattggcatg acaacatagg cagccgactg tgatgcggca
     3421 tcttcaatct gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcacatttc
     3481 agcgcgttca gaacatccag cacgtagctg acgttgaagc cgatttccat ctccgcaccg
     3541 ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcggtgatt
     3601 ttcagctggt tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatc
     3661 gccgcgcgag caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg
     3721 ttcttcggca gaacgcggcg ataatccggg aagcgaccat ccaccagttt ggaggtgaag
     3781 ataaagtcgc caacgtgggc gcgaatgttg ttgctgccaa tctgtacgcg cagcggattg
     3841 tcgccgccgt cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa
     3901 tggcttggca aagattgacc aattggcatt gaacagactg ccagacggtg gccgtcggtc
     3961 gccacggtgc gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga
     4021 acgtcctgat gcgccataga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc
     4081 ggcagggtaa attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc
     4141 agggtagaca gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc
     4201 tgtacggcaa tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc
     4261 ggaacggtcg tcgctcctgg ctcgtgtggc tgaaccagcg caacgcgtgc caccatttcc
     4321 atctcgagat cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta
     4381 ccgagaatcg gtagcgtagg acgaccacct aacggcccgc tcacctgttg tagcggtttt
     4441 aataaatgct cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa
     4501 atttgaaaaa tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca
     4561 ggcatgaagc accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact
     4621 gtggttagtc agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg
     4681 ggatcgacgc ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt
     4741 ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagtgcctc
     4801 acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg gcgttagcaa taacgcggtt
     4861 cagcgcccct tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac
     4921 ttcgcctggc aaacgaatgt cattttcgtc ggcctttttc atcaggatcg ccacgcgggt
     4981 ttccagctct ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg
     5041 atcctcaacg ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt
     5101 accttccagc agggcgttga aggtgtggaa aaactcttcc tgagatcgtt ctttattagc
     5161 aaaaaactga atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc
     5221 ttcgatcgcg ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat
     5281 ataaaccact ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag
     5341 gtgagtttta cccagacccg tgccgccata aaggaacaat gggttatagg caccgccagg
     5401 gttatccgcc acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa
     5461 gttatcaaac gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg
     5521 gacgttatcc caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac
     5581 ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt
     5641 ggtgccgact tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc
     5701 attgatatta ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta
     5761 cagggccagc gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc
     5821 tgtggctggt aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac
     5881 ggcggactcc actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg
     5941 acgtacgtcg aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg
     6001 cccgatcaag atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag
     6061 atcttctgtt tctcacagat tttcccgatt tatccacagg actttccaga cctcgctaag
     6121 tgtaaacgat cctgccgcga ggcgggcacg atttacgccg catattggaa aatttaatga
     6181 ccatagacaa aaattggctt aatcgatcta ataaagatct aggacgatcc ttgcgcttta
     6241 cccatcagcc cgtataatcc tccacccggc gcgccatgct ggttttcact ggtgtgaggt
     6301 cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac
     6361 tccggagtgt acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat
     6421 cgtttcattt ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa
     6481 atcgccatga aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc
     6541 cgtgctcgta tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc
     6601 cgcgctcgtc tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt
     6661 tcccagggag ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca
     6721 acgggctggc acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg
     6781 tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa
     6841 acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt
     6901 ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga
     6961 aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc
     7021 tatcaacgcc tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca
     7081 agctacggaa ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg
     7141 aaacgcgtat taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga
     7201 ccatttgata ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt
     7261 tgctgttcgt gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc
     7321 aggcccaaca gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg
     7381 taccggccag tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca
     7441 tcaacacccg tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga
     7501 actctaccca gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga
     7561 gcggtctgac cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata
     7621 acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga
     7681 cgtataccga cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt
     7741 acgctgtcaa cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca
     7801 cctttggtca gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca
     7861 acttcgcact gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga
     7921 aatacaagtt cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt
     7981 gggtggcaat gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca
     8041 acaacttcta taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc
     8101 cggtactggt tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg
     8161 aaatccagga caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt
     8221 ggttgtggtt catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg
     8281 gtaactgggg cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc
     8341 tgaccaaagc gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg
     8401 caatgcgtga gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt
     8461 acaaagctga gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa
     8521 tcttcctggc gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg
     8581 cactgtggat ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg
     8641 gcgtaacgat gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc
     8701 agaagatcat gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag
     8761 gtctggtgct gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt
     8821 accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg
     8881 agttttcgct aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggtggata
     8941 agcaccgcgc atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg
     9001 tagcccaggc cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcctaa
     9061 aagcccgtga agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt
     9121 atcttccgtt taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc
     9181 ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact gcagggtcat ggcggtccgg
     9241 tgatcctcga cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc
     9301 ctggtgagtt ttccgaacgc gcgtttctta acgataaact cgacttagcc caggccgagg
     9361 cgattgccga tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc
     9421 tgcaaggcgc attctccgca cgggtcaacc atctggtgga agccctcacc cacttgcgca
     9481 tttacgtcga agcggcaatt gatttccctg atgaagagat cgatttcctc tccgacggca
     9541 aaattgaagc ccagcttaat gacgtgattg ccgatctcga tgcagtgcgt gctgaagcac
     9601 gtcagggtag tttgttgcgc gaagggatga aggtggtgat tgccggacgt cctaacgccg
     9661 gtaaatcgag cctgttaaac gcgctggcgg gccgtgaagc agcaatcgta actgatatcg
     9721 ccggaaccac acgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata
     9781 tcatcgatac cgctgggcta cgtgaagcca gtgacgaagt ggaacgtatt ggtatcgagc
     9841 gcgcgtggca ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa
     9901 cagacgccgt ggatccggca gagatctggc cggaatttat cgcccgtctg ccagcgaaac
     9961 taccgatcac cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg
    10021 aagtgaacgg tcacgcgtta attcgtctct cggcaaggac aggtgaaggc gtggaggtgc
    10081 tgcgtaacca tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg
    10141 cgcgtcgtcg ccacctacag gcgctggaac aggcagcgga gcatttgcag caaggtaaag
    10201 cgcaactgtt aggtgcctgg gcgggtgaac tgctggcgga agagttgcgc ctggcacagc
    10261 agaacttaag cgaaatcacc ggggaattta cctctgatga tttgctgggg cggattttct
    10321 ccagcttctg tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg
    10381 gcttcgcttc attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt
    10441 cttacgtaat ttataatctt taaaaaaagc atttaatatt gctccc
//
LOCUS       DAJVXP010000139        10430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVXP010000139 DAJVXP010000000
VERSION     DAJVXP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.139"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OQS10_003406"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OQS10_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS2294351.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OQS10_003407"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OQS10_003407"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS2294352.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OQS10_003408"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OQS10_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCS2294353.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OQS10_003409"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OQS10_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS2294354.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OQS10_003410"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OQS10_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OQS10_003411"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OQS10_003411"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS2294355.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OQS10_003412"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OQS10_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS2294356.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OQS10_003413"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OQS10_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS2294357.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OQS10_003414"
     CDS             complement(9457..10149)
                     /locus_tag="OQS10_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS2294358.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2516 c         2755 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAJVXP010000140        10363 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVXP010000140 DAJVXP010000000
VERSION     DAJVXP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.140"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..154
                     /locus_tag="OQS10_003415"
     CDS             <1..154
                     /locus_tag="OQS10_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2294359.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OQS10_003416"
     CDS             258..1172
                     /locus_tag="OQS10_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2294360.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OQS10_003417"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OQS10_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS2294361.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OQS10_003418"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OQS10_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS2294362.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OQS10_003419"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OQS10_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS2294363.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OQS10_003420"
     CDS             5467..7149
                     /locus_tag="OQS10_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS2294364.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OQS10_003421"
     CDS             7201..8358
                     /locus_tag="OQS10_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS2294365.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OQS10_003422"
     CDS             complement(8324..8446)
                     /locus_tag="OQS10_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294366.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OQS10_003423"
     CDS             8655..10340
                     /locus_tag="OQS10_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCS2294367.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3029 a         2208 c         2213 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg tac
//
LOCUS       DAJVXP010000141        10345 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVXP010000141 DAJVXP010000000
VERSION     DAJVXP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.141"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(44..1048)
                     /gene="cra"
                     /locus_tag="OQS10_003424"
     CDS             complement(44..1048)
                     /gene="cra"
                     /locus_tag="OQS10_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS2294368.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1130..1216)
                     /locus_tag="OQS10_003425"
     CDS             complement(1130..1216)
                     /locus_tag="OQS10_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS2294369.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1228..1719)
                     /gene="ilvN"
                     /locus_tag="OQS10_003426"
     CDS             complement(1228..1719)
                     /gene="ilvN"
                     /locus_tag="OQS10_003426"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS2294370.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1722..3446)
                     /gene="ilvI"
                     /locus_tag="OQS10_003427"
     CDS             complement(1722..3446)
                     /gene="ilvI"
                     /locus_tag="OQS10_003427"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS2294371.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3565..3609
                     /locus_tag="OQS10_003428"
     CDS             3565..3609
                     /locus_tag="OQS10_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294372.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3764..4708)
                     /gene="leuO"
                     /locus_tag="OQS10_003429"
     CDS             complement(3764..4708)
                     /gene="leuO"
                     /locus_tag="OQS10_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS2294373.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5368..5454
                     /gene="leuL"
                     /locus_tag="OQS10_003430"
     CDS             5368..5454
                     /gene="leuL"
                     /locus_tag="OQS10_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS2294374.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5547..7118
                     /gene="leuA"
                     /locus_tag="OQS10_003431"
     CDS             5547..7118
                     /gene="leuA"
                     /locus_tag="OQS10_003431"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS2294375.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7118..8209
                     /gene="leuB"
                     /locus_tag="OQS10_003432"
     CDS             7118..8209
                     /gene="leuB"
                     /locus_tag="OQS10_003432"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS2294376.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8212..9612
                     /gene="leuC"
                     /locus_tag="OQS10_003433"
     CDS             8212..9612
                     /gene="leuC"
                     /locus_tag="OQS10_003433"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS2294377.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9623..10228
                     /gene="leuD"
                     /locus_tag="OQS10_003434"
     CDS             9623..10228
                     /gene="leuD"
                     /locus_tag="OQS10_003434"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS2294378.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2712 a         2719 c         2525 g         2389 t
ORIGIN      
        1 gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc ggcttagcta cggctgagca
       61 caccgcggcg atagagatta cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt
      121 cgtccaggct tgccaggaca atctccagca cacgctctgc gacatcgcgg tgacgttgag
      181 ccactgccag caccgggcac tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa
      241 ttgccaggtc agaaggcagt ttaccatcgc gacgtagcgt gacatccatc accccttgca
      301 acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg atgcgtttcc agccattttt
      361 cgaataactg ggcagccacc tcccgctcat agctgttggc atacaggaaa tgcacttcgc
      421 gcggatcatc tttccaggca gtacggaaac cttgttcacg caggaagctg acagaaagct
      481 ccggcagcgc gccaagataa agcaccgtct cggcgggaaa cttacgtaac tcttccgcca
      541 gcatttcggc atcatcctga tcggcaccaa ccacgctggt gaagtgttca cgatcgaggg
      601 cgcggtccag cgcgacaatc gggaacgggt cgttagccca gcgttgataa aaaggatgct
      661 caggaggcaa cgacgtcgaa acaataatgg catcgacctg acgctgtaaa aggtgctcaa
      721 tacagcgcat ttcgttgtct ggctgatctt ctgagcaggc aattagcagt tgataacccc
      781 gttgccgcgc ctggcgttca agatagttag cgatgcgggt atagctggtg ttctccagat
      841 cggggatcac aagaccaata gaacgtgtgc gtccagcacg aagcccagct gccacggcgt
      901 tcgggtggta attgtgctca cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca
      961 cacggtattg cttcgctttg ccgttaataa catagcttgc agtggtccgc gacactcccg
     1021 ccagccgagc gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata
     1081 gccattgtac tggtatgggt taaattcatt aacatctaag cgcataaaat taactgcggc
     1141 aactgctttt attccaggtt cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg
     1201 gctgcaaatt gcgcattcag atcattctca acgcattatt ttatcgccgc gcgaaagtcc
     1261 gaccacacca gaacgagcaa cttccacaat tttcgccacc tcgcgaatcg atgctaaaaa
     1321 tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc
     1381 gataatttgc ccacggaata tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct
     1441 ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc gcgccctgcc ccaactcact
     1501 cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg atctgctcaa gtactttttc
     1561 atcgcccacg gtctggatgg tcatacgcga taatgtcgga tcatcggttg acgcaacggt
     1621 caggctttca atgttgtagc cacgctggga aaaaaggcca atcacgcggg ataacgcgcc
     1681 tgattcattt tcgagtaaga ctgataatat ccggcgcata atcaggttct ctccgttttg
     1741 cttaaccaca tttcatccat tccgcccccg cgaatctgca tcgggtagac gtgctcgctg
     1801 ccatcgacgg taacatcaac aaacaccagg cgattattgt gcacctgttc cagcgcctcg
     1861 ctaagtttgc tttccagctc atgcggatga gaaatctgga tcccgacgtg accataggct
     1921 tccgccagac ggacgaaatc gggtagcgat tgcatataag attgtgaatg acggccggaa
     1981 tagatcatgt cctgccactg cttcaccatc cccagatagc ggttattgag attcaccacc
     2041 agtacgggca actcgtattg caacgcggta gacagttcct ggatgttcat ctgaatactg
     2101 ccgtcgccag tgacgcaaac cacggtttct tctggcaacg ccattttgac gcccagtgcc
     2161 gcaggtaaac caaaacccat cgtgccgagg ccaccggaat tgatccagcg acgcggtttg
     2221 tcgaatggat aataaagtgc agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa
     2281 gcgtctccct tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca
     2341 ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatttg ctgccaccag
     2401 tcgcggatct catccagtgg ttgatgggcg gattcttgcg acaagagttc aagcatttgt
     2461 tcgaggacct ggcgagcatc ccccacaatc gggatatccg cagtcacggt tttagaaatg
     2521 gaagtaggat caatatcgat atgcagaaca gtggcatttg ggcagtactt tgccagattg
     2581 ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc
     2641 gtcatattgg cttcgtaggt accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt
     2701 gccggaaacg ctcccagccc catcaatgag gaaacaacgg gcagattcaa cgcctccacc
     2761 gtttctttca actgctgatg gcagcccgcc gtgattgccc caccgcctac gtagacaacc
     2821 ggtttttttg ccgctaccag cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta
     2881 gtagtgggat tgtaagaacg catactgacc gactccggcc agacataggg taatttgttc
     2941 gccggattaa gaatatcttt cggtaaatca acgactactg gtccaggacg accacttgcc
     3001 gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa
     3061 ctgtgtttaa ccactggtcg cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa
     3121 cctatcaacg aggtcgctac ctgcccggaa aggacaacta atggaatgga atccatataa
     3181 gcggtggcga tgccagtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg
     3241 ccgacttccc cggtcgcgcg cgccagacca tcggccatat gcactgccgc ctgctcatga
     3301 cgaactaaaa catgatcgat accacccacg gtatgcaatg catcgtaaat atcaaggact
     3361 gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc
     3421 atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta
     3481 aaacacatta accgtccgat cttgcttaag caattggcag ccagagacat aaacgcaggg
     3541 taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacgaagga
     3601 ttaaattgat aataaatcgg ctgaatccca caacttacag caatttctct atttttttaa
     3661 ttaatcctaa aatagagagc ccacacattc agaataaaat tctacattca ataaaaaata
     3721 cagaataaac cagacattca tgtctgacct attctgcatt cagttagcgt ttgcaaattg
     3781 agactaattg ctcttccatc cactgatggc ctttatcgcg cccggcagct tcatgccagg
     3841 agagataaca ggttctgcta ttttgtttta acggcagcgg taatacctgt aattctaagg
     3901 attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca
     3961 ccacgctaag tacgctcatc attgccatgc cctgatacgc gatactggct tgcttatcta
     4021 ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt
     4081 gttcgttata aacatcatgt ttcagtaacg ggcccttaat tgttggatga tttttgctgg
     4141 ctaccagcac catttcatct ttaaataatg gcacgctggt aaattcagga cgatggaagt
     4201 cttcataact aatcacaaac tccgtttcct gataacgcag ctgatgttca gtgttctgat
     4261 ttaatgaaga cttgaacata acatgtatat ttggcgcaat ctgctcaatg tgattataaa
     4321 tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca aagatgaaat acacgttcac
     4381 tgctcgcggg ttcaaaacct gaaccaggca attcattttg tactagttgc aatgcctgac
     4441 gaactgagcc aaaaagttga aatgcgcgag cagtcggttg aataccacgg ccataacgaa
     4501 caaaaagctc gtcattaaac atcaccttca ggcgtgcaac agcgttactg accgcaggtt
     4561 gcgacatacc cagaacatga gcggcacggg taatgttttg ctcctgcatc acggcatcga
     4621 aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct
     4681 ctgaatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt aactcccttt
     4741 cccttattgg aatgcagaga aataatcctg aagaatatga tttatcatgc atataaaata
     4801 agaaaaagca aaatgagtaa aattcggaaa acataaagat acgggttctg ataaaaacag
     4861 aatccattgc cgtggaatga gtcatttacc acagcataag aatccataat gtttttgttg
     4921 cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca tctacaaaat ggattaaatg
     4981 tgcttattta ataattaatt tatatattta atgcattaat tcttaacatt aattgatcaa
     5041 taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca tataattcaa tacaaatcat
     5101 caggataggt tttgcaacgc gtgcattttg tccccttttt cctcgttgat tagatgcaaa
     5161 aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt agttaagcag aaattaatat
     5221 cgcttacttt aaccaccgca gcacaattag ctaattttac ggatgcagaa ctcacgctgg
     5281 cgggacgttt ttattgcgtc agggttgaca tccgtttttg tatccagtaa ctctaaaagc
     5341 atatcgcatt catctggagc tgatttaatg actcacatcg ttcgctttat cggtctacta
     5401 ctactaaacg catcttcttt gcgcggtaga cgagtgagcg gcatccagca ttaagccagc
     5461 acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt tttatgcccg aagcgaggcg
     5521 ctctaaaaga gacaaggacc taaaccatga gccagcaagt cattattttc gataccacat
     5581 tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt gaaagaaaaa ctgcaaattg
     5641 cgctggccct tgagcgtatg ggtgttgacg tgatggaagt cggtttcccc gtctcttcgc
     5701 cgggcgattt tgaatcggtg caaaccatcg cccgccaggt taaaaacagc cgcgtatgtg
     5761 cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc cgaatccctg aaagtcgccg
     5821 aagccttccg tattcatacc tttattgcta cctccccaat gcacatcgcc accaagctgc
     5881 gcagcaccct ggacgaggtg atcgaacgcg ctatctatat ggtgaaacgc gcccgtaatt
     5941 acaccgatga tgttgaattt tcttgcgaag atgccgggcg tacacccatt gccgatctgg
     6001 cgcgagtggt cgaagcggcg attaatgccg gtgccaccac catcaacatt ccggacaccg
     6061 tgggctacac catgccgttt gagttcgccg gaatcatcag cggcctgtat gaacgcgtac
     6121 ctaacatcga caaagccatt atttccgtac atacccacga cgatttaggc ctggcggtcg
     6181 gcaactcact ggcggcggta catgccggtg cacgccaggt ggaaggtgca atgaacggga
     6241 tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat catggcgatt aaagttcgta
     6301 aggatattct caacgtccac accgccatta atcaccagga gatatggcgc accagccagt
     6361 tagttagcca gatttgtaat atgccgatcc cggcaaacaa agccattgtt ggcagcagcg
     6421 cattcgctca ctcctccggt atccaccagg atggcgtgct gaaaaaccgc gaaaactacg
     6481 aaatcatgac accagaatct attggtctga accaaatcca gctgaatctg acctctcgtt
     6541 cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg gtataaagaa agtgaatata
     6601 atttagacaa tttgtacgat gctttcctga agctggcgga caaaaaaggt caggtgtttg
     6661 attacgatct ggaggcgctg gccttcatcg gtaagcagca agaagagccg gagcatttcc
     6721 gtctggatta cttcagcgtg cagtctggct ctaacgatat cgccaccgcc gccgtcaaac
     6781 tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg taacggtccg gtcgatgccg
     6841 tctatcaggc aattaaccgc atcactgact ataacgtcga actggtgaaa tacagcctga
     6901 ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga tattgtcgcc aactacaacg
     6961 gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt cgagtcctcc gccaaagcca
     7021 tggtgcacgt actgaacaat atctggcgtg ccgcagaagt cgaaaaagag ttgcaacgca
     7081 aagctcaaca caacgaaaac aacaaggaaa ccgtgtgatg tcgaagaatt accatattgc
     7141 cgtattgccg ggggacggta ttggtccgga agtgatgacc caggcgctga aagtgctgaa
     7201 tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc cattacgatg taggcggcgc
     7261 agccattgat aaccacgggc aaccactgcc gcctgcgacg gttgaaggtt gtgagcaagc
     7321 cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg gaacatttac caccagacca
     7381 gcaaccagaa cgcggcgcgc tgctgcctct gcgtaagcgc ttcaaattat tcagcaacct
     7441 gcgcccggca aaactgtatc aggggctgga agcattctgt ccgctgcgtg cagacattgc
     7501 cgctaacggc ttcgacatcc tgtgcgtgcg cgaactgacc ggcggcatct atttcggtca
     7561 gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg tttgataccg aggtgtatca
     7621 ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa tctgctcgta aacgtcgcca
     7681 caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc tctattttat ggcgtgaaat
     7741 cgtcaacgag atcgccacgg aatacccgga tgtcgaactg gcgcatatgt acatcgacaa
     7801 cgccaccatg cagctgatta aagatccatc acagtttgac gttctgctgt gctccaacct
     7861 gtttggcgac attctgtctg acgagtgcgc aatgatcact ggctcgatgg ggatgttgcc
     7921 ttccgccagc ctgaacgagc aaggttttgg tctgtatgaa ccggcaggcg gctcagcacc
     7981 agatatcgca ggcaaaaaca tcgccaaccc gattgcgcaa attctgtcgc tggcactgct
     8041 gctgcgctac agcctggatg ccgatgatgc ggcttgcgcc attgaacgcg ccattaaccg
     8101 cgcattagaa gaaggcattc gcaccgggga tttagcccgt ggcgctgccg ccgttagtac
     8161 cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa ggggtgtaat catggctaaa
     8221 acgttatacg aaaaattatt cgacgctcac gtagtgtacg aagccgaaaa cgaaaccccg
     8281 ctgttatata tcgaccgcca cctggtgcat gaagtgacct caccgcaggc gttcaatggt
     8341 ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa ccttcgccac tatggatcac
     8401 aacgtctcta cccagacgaa agacattaat gcctgtggtg aaatggcgcg catccagatg
     8461 caggagctga ttaaaaactg caaagaattt ggcgtcgaac tgtatgacct gaatcacccg
     8521 tatcagggga tcgtccacgt aatggggccg gaacagggcg tcaccttgcc ggggatgacc
     8581 attgtctgcg gcgactcgca taccgccacc cacggcgcgt ttggcgcact ggcctttggt
     8641 atcggcactt ccgaagttga acacgtactg gcaacgcaaa ccctgaaaca gggccgcgca
     8701 aaaaccatga aaattgaagt ccagggcaaa gccgcgccgg gcattaccgc aaaagatatc
     8761 gtgctggcaa ttatcggtaa aaccggtagc gcaggcggca ccgggcatgt ggtggagttt
     8821 tgcggcgaag caatccgtga tttaagcatg gaaggtcgta tgaccctgtg caatatggca
     8881 atcgaaatgg gcgcaaaaac cggtctggtt gcaccggacg aaaccacctt taactatgtc
     8941 aaaggccgtt tgcatgcgcc gaaaggcaaa gatttcgacg acgccgttgc ctactggaaa
     9001 accctgcaaa ccgacgaagg cgcaactttc gataccgttg tcactctgca agcagaagaa
     9061 atttcaccgc aggtcacctg gggcaccaat ccaggccagg tgatttccgt gaacgacaat
     9121 attcccgatc cggcttcgtt tgccgatccg gttaaacgcg cgtcggcaga aaaagcgctg
     9181 gcctatatgg ggctgaaacc gggcattccg ctgaccgaag tggctatcga caaagtgttt
     9241 atcggttcct gtaccaactc gcgcattgaa gatttacgcg cggcagcgga gatcgccaaa
     9301 gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc ccggctctgg cccggtaaaa
     9361 gcccaggcgg aagcggaagg tctggataaa atctttattg aagccggttt tgaatggcgc
     9421 ttgcctggct gctcaatgtg tctggcgatg aacaacgacc gtctgaatcc gggcgaacgt
     9481 tgtgcatcca ccagcaaccg taactttgaa ggccgccagg ggcgcggcgg gcgcacgcat
     9541 ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg gacatttcgc cgacattcgc
     9601 aacattaaat aaggagcaca ccatggcaga gaaatttatc aaacacacag gcctggtggt
     9661 tccgctggat gccgccaatg tcgataccga tgcaatcatc ccgaaacagt ttttgcagaa
     9721 agtgacccgt acgggttttg gcgcgcatct gtttaacgac tggcgttttc tcgatgaaaa
     9781 aggccaacag ccaaacccgg acttcgtgct gaacttcccg cagtatcagg gcgcttccat
     9841 tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt gagcacgcgc cctgggcatt
     9901 gaccgactac ggctttaaag tggtgattgc gccgagtttt gctgacatct tctacggcaa
     9961 tagctttaac aaccagctgc tgccggtgaa attaagcgat gcagaagtgg acgaactgtt
    10021 tgcgctggtg aaagccaatc cggggatcca tttcgacgtg gatctggaag cgcaagaggt
    10081 gaaagcggga gagaaaacct atcgctttac catcgatgcc ttccgccgcc actgcatgat
    10141 gaacggtctg gacagtattg ggcttacctt gcagcacgac gacgccattg ccgcttatga
    10201 agcaaaacaa cctgcgttta tgaattaatc cccttgcccg gtcaaatgac cgggctttcc
    10261 gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt taaaaataat gcgacaacag
    10321 aaataaccgc aattacccag tagac
//
LOCUS       DAJVXP010000142         9855 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVXP010000142 DAJVXP010000000
VERSION     DAJVXP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.142"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OQS10_003435"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OQS10_003435"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS2294379.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OQS10_003436"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OQS10_003436"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS2294380.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OQS10_003437"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OQS10_003437"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS2294381.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OQS10_003438"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OQS10_003438"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS2294382.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OQS10_003439"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OQS10_003439"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS2294383.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5060..6193
                     /locus_tag="OQS10_003440"
     CDS             5060..6193
                     /locus_tag="OQS10_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS2294384.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OQS10_003441"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OQS10_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS2294385.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OQS10_003442"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OQS10_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS2294386.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OQS10_003443"
     CDS             complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OQS10_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS2294387.1"
                     /translation="MVKKTIAAIFSVLVLSTVLNACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OQS10_003444"
     CDS             complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OQS10_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS2294388.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OQS10_003445"
     CDS             complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OQS10_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS2294389.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8346..9374
                     /gene="epmB"
                     /locus_tag="OQS10_003446"
     CDS             8346..9374
                     /gene="epmB"
                     /locus_tag="OQS10_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS2294390.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9764..>9855
                     /locus_tag="OQS10_003447"
     CDS             9764..>9855
                     /locus_tag="OQS10_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294391.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2520 a         2505 c         2501 g         2329 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacccggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttgcca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cgttactcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactcaagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc ggcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcacgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag
     2221 gcacgctggt gcagatccgt gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctggttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgggccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctagag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg tctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggcg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtatcgttac aatctaacgt atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttactgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 aacccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg
     6361 gcgtgacagt agtgaataat ttcgacgcta aacgctatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagtctgc
     6481 gtgatgacgg cggcctgaat gtcatcaata aaggctataa ccctgacaga gaaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgtcattgc acttgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacagtaat aacacttggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gagaattggg gccatccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt gtgactatcg
     7261 caaaaaaaca aacggcacga cataactgtc gtaccgttat tgcttattgc tgcgctttcg
     7321 ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     7381 tgttgcaggc atttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     7441 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     7501 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     7561 ttagctggca gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg
     7621 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     7681 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     7741 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     7801 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     7861 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     7921 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     7981 cagcagccat ttagcgttgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     8041 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     8101 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     8161 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     8221 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     8281 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     8341 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     8401 ttgccgatgt tgtgaccgat cctggtgaac ttctgcgtct tttgaatata gacgcggacg
     8461 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     8521 ttatcgatcg catggagaaa ggcaatccgg acgatcctat tttgcgtcag gtacttacct
     8581 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     8641 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     8701 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     8761 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     8821 agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc
     8881 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     8941 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     9001 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     9061 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     9121 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     9181 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     9241 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     9301 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     9361 tacgccagca gtaactgatt aaacagggaa gttaaacatt agcctgatgc gctacgctta
     9421 tcaggcctac attgtgcatt gaaattgaat ggatttgcaa tgaactgtag gccggataag
     9481 gcgtttacgc cgcatccggc aagagcaaca aacgcaagta cagcaacctg tcattaaact
     9541 gggaaattta atctttgccc gttgcataac ctttaattat tcatatacat tgctgttgtt
     9601 actctttata tagccaacac caataattaa cctttctttt ctgtttatat agagtgagta
     9661 cggctattta tgttatttta acaactatta ctaccaagcc catttatctg gcttataaat
     9721 taaaattaat tatttaattg agaaatggtt agggagaacc tacatggcca taagcatcaa
     9781 gggtgttaat accggtgtta ttcgcaagag taataatttc attgcactgg cgctgaaaat
     9841 taaagaacct cgcaa
//
LOCUS       DAJVXP010000143         9811 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVXP010000143 DAJVXP010000000
VERSION     DAJVXP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.143"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(39..1034)
                     /gene="gapA"
                     /locus_tag="OQS10_003448"
     CDS             complement(39..1034)
                     /gene="gapA"
                     /locus_tag="OQS10_003448"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS2294392.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1376..1789
                     /gene="msrB"
                     /locus_tag="OQS10_003449"
     CDS             1376..1789
                     /gene="msrB"
                     /locus_tag="OQS10_003449"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS2294393.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1831..2103
                     /locus_tag="OQS10_003450"
     CDS             1831..2103
                     /locus_tag="OQS10_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS2294394.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="OQS10_003451"
     CDS             complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="OQS10_003451"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS2294395.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="OQS10_003452"
     CDS             complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="OQS10_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS2294396.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="OQS10_003453"
     CDS             complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="OQS10_003453"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS2294397.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6116..6667
                     /locus_tag="OQS10_003454"
     CDS             6116..6667
                     /locus_tag="OQS10_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS2294398.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6784..7827
                     /gene="selD"
                     /locus_tag="OQS10_003455"
     CDS             6784..7827
                     /gene="selD"
                     /locus_tag="OQS10_003455"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS2294399.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7832..9793
                     /gene="topB"
                     /locus_tag="OQS10_003456"
     CDS             7832..9793
                     /gene="topB"
                     /locus_tag="OQS10_003456"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS2294400.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2432 a         2450 c         2615 g         2314 t
ORIGIN      
        1 ccgaagtcgc tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc
       61 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
      121 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
      181 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
      241 tcagcagcgg ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
      301 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
      361 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
      421 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
      481 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
      541 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
      601 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
      661 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
      721 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
      781 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
      841 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
      901 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
      961 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     1021 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     1081 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     1141 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg
     1201 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     1261 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     1321 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     1381 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     1441 gcagaatcat gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     1501 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc
     1561 cggctgtggt tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     1621 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     1681 ggggcatgtc ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc
     1741 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     1801 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     1861 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcccgatggc
     1921 gttgcgttga ccgaggaaca aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct
     1981 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     2041 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag
     2101 tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata cttgttagga
     2161 cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca
     2221 atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt
     2281 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgcg
     2341 cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcaca tttaccttat
     2401 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     2461 taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgag gttttctgcc
     2521 gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     2581 acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct
     2641 gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag
     2701 gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg
     2761 cgataaccgc ttcaccgcgc gactggcacc agtcaatcag gcggttagcg acatccaccg
     2821 tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     2881 ccagtaacag ggcgcgaggg ggcattacag tctccttaat catccggcgt cagttcgccg
     2941 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     3001 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca
     3061 tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt
     3121 gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct
     3181 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
     3241 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac tacgccaatt
     3301 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     3361 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     3421 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     3481 aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga
     3541 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     3601 ggtttaccga gattctcgag catgaacgac agcgcagagg cagtatacgc catcgtgtcg
     3661 gtgccgtgca ggatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     3721 atatgctgcc agtcttctgg cgtcatatca gaagaatcca tcagcggcgt atattcatga
     3781 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     3841 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     3901 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     3961 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     4021 gggcgagaca gcgggaagag gattcgtcga cgtaaaaaat aatgtggccg acgatctgtc
     4081 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     4141 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     4201 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     4261 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     4321 ccactgtttc acttttgcca gctctgctgc tttggcgacc gcatcatcga aatccccgag
     4381 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     4441 tttatcaatc tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     4501 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     4561 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     4621 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     4681 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     4741 gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg
     4801 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     4861 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     4921 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     4981 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     5041 atcagtctta ctccagccga actctttggt tagagctttt tcgatttcgg cactcgatgc
     5101 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     5161 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
     5221 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     5281 gcgggctgcc ggtgacatgt catcacgaat aaacggttca acggcagatt tatacgtacc
     5341 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     5401 gttggtagca aaaccgtgca aatccaccac gccttgcggt gacagccaaa ttttattggc
     5461 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     5521 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     5581 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
     5641 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     5701 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac
     5761 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     5821 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     5881 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     5941 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     6001 gtggttatcg taatgacaca accggcactt gtcagcagga attgcggact gtgctgtaac
     6061 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
     6121 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     6181 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     6241 gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact
     6301 ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc
     6361 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt
     6421 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     6481 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     6541 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     6601 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     6661 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     6721 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     6781 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     6841 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     6901 ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat
     6961 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     7021 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     7081 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
     7141 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
     7201 cactccatcg atgcgacgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
     7261 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
     7321 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag
     7381 ggactagcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
     7441 gaaggcgtaa aagcgatgac cgacgttacg ggttttggtc tgctgggcca tttgagcgaa
     7501 atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg
     7561 ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc
     7621 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg
     7681 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct
     7741 acagccgccg agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc
     7801 ggtcgtgcca tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag
     7861 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
     7921 cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca
     7981 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattatccc
     8041 ggaaaagtgg caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg
     8101 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact
     8161 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
     8221 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgctcg accgtcttcg
     8281 gtccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
     8341 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
     8401 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
     8461 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
     8521 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
     8581 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
     8641 tggtcaacca gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
     8701 gcctttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca
     8761 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
     8821 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
     8881 catcagcgtt catgcaccag atctgttgct gcagccagtg gtagatccag atatacgcaa
     8941 ccgctgttgg gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag
     9001 ttcagccatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta
     9061 cttgatgcag ttctgcccgg atgtggtgtt ccgcaagtgt gttatcgaac tggacattgc
     9121 caaaggcaaa tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt
     9181 aggcagcaaa gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg
     9241 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
     9301 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
     9361 agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
     9421 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
     9481 taccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
     9541 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
     9601 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
     9661 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
     9721 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg
     9781 ggcgatagcg taagcgagtg aatctttcgt g
//
LOCUS       DAJVXP010000144         9548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVXP010000144 DAJVXP010000000
VERSION     DAJVXP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.144"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1327
                     /locus_tag="OQS10_003457"
     CDS             <1..1327
                     /locus_tag="OQS10_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS2294401.1"
                     /translation="IIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGF
                     HYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDC
                     ARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTL
                     RVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDV
                     DHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNP
                     AEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGF
                     QNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRV
                     RVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1330..2658
                     /gene="ssnA"
                     /locus_tag="OQS10_003458"
     CDS             1330..2658
                     /gene="ssnA"
                     /locus_tag="OQS10_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCS2294402.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2709..3488
                     /locus_tag="OQS10_003459"
     CDS             2709..3488
                     /locus_tag="OQS10_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCS2294403.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3485..6424
                     /locus_tag="OQS10_003460"
     CDS             3485..6424
                     /locus_tag="OQS10_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCS2294404.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6357..6671
                     /locus_tag="OQS10_003461"
     CDS             6357..6671
                     /locus_tag="OQS10_003461"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS2294405.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6707..7057
                     /gene="ghxQ"
                     /locus_tag="OQS10_003462"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6707..7057
                     /gene="ghxQ"
                     /locus_tag="OQS10_003462"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7126..7614)
                     /locus_tag="OQS10_003463"
     CDS             complement(7126..7614)
                     /locus_tag="OQS10_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS2294406.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVPTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7614..9533)
                     /gene="ygfT"
                     /locus_tag="OQS10_003464"
     CDS             complement(7614..9533)
                     /gene="ygfT"
                     /locus_tag="OQS10_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCS2294407.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2303 a         2560 c         2489 g         2196 t
ORIGIN      
        1 cattattcct cagttccgta ttcctgcaga gttaattcag cacgatatcg attttgttgc
       61 cgctcacggc gtgaaatttg agtatggctg ctcacccgat ttaaccgttg aacagttaaa
      121 aaatcagggc ttccactatg ttctgattgc caccggcact gataaaaata gcggtgtgaa
      181 actggcgggc gacaaccaaa atgtctggaa atcactcccc ttcctgcgtg aatacaacaa
      241 gggtacagcg ctcaagctgg gcaaacatgt ggtcgttgtc ggggcgggta acaccgcaat
      301 ggactgcgct cgtgcggcgt tacgcgttcc aggcgtagaa aaagcaacga tcgtttaccg
      361 tcgttcacta caagagatgc ccgcatggcg cgaagagtat gaagaagcgt tgcacgacgg
      421 cgtagagttc cgtttcctga ataatccgga acgtttcgat gctgatggca ccttaacctt
      481 gcgcgttatg tcgcttggcg aaccggatga gaaaggtcgt cgccgtccgg ttgaaaccaa
      541 cgaaacagta acactgcatg tagacagcct gatcaccgcc attggtgaac agcaggatac
      601 tgaagccctg aatgcgatgg gcgtgccgct ggacaaaaac ggctggccag acgtcgacca
      661 taatggcgaa actcgtctga ctgacgtctt tatgatcggc gacgtacagc gcggaccatc
      721 ctccattgtc gctgctgtcg gaaccgcgcg tcgggcgacc gatgccatcc ttagtcggga
      781 aaatatccgt tcccaccaga acgataaata ctggaacaac gtcaatccag cggaaatcta
      841 tcaacgtaaa ggcgatatct ctatcacgct ggtgaacagt gacgatcgtg acgcgtttgt
      901 cgcccaggaa gccgctcgct gcctcgaatg taactacgtt tgcaacaagt gtgtggatgt
      961 ctgcccgaac cgcgccaacg tctccattgc agtccctggc ttccagaata gattccagac
     1021 gctgcacctc gacgcttact gtaacgaatg cggcaactgc gctcagttct gtccgtggaa
     1081 cggtaaacca tacgaagaca aaatcaccgt cttcagcctg gcgcaagact ttgataacag
     1141 cagcacccca ggcttccttg tggaagattg ccgggtacga gtacgtctga ataaccaaag
     1201 ctgggtgtta aacatcgaca gcgaaggtca gtttaacaac gtaccaccgg agctgaacga
     1261 tatgtgccgc atcatcagcc atgtccacca gcatcatcat tatctgctgg gccgcgtgga
     1321 ggtgtaatca tgttgattct gaagaatgtc accgcagtgc agttacaccc ggcgaaagtg
     1381 caggaaggcg ttgatatcgc tatcgaaaat gacgtgattg tcgctatcgg cgatgccctg
     1441 acgcaacgct accccgatgc cagctacaaa gagatgcatg gtcggattgt gatgccggga
     1501 attgtctgct cgcacaacca tttttactcg gagctttccc gcggaattat ggcaaacatc
     1561 gccccttgcc cggatttcat ctcaacgctg aaaaatctct ggtggcggct cgatcgcgcc
     1621 cttgatgaag agtcgctcta ttacagcgga ctgatttgtt ccctggaagc gattaagagc
     1681 ggatgtacat cggttatcga tcaccatgcc tcgccggcgt atatcggcgg gtcgctctcc
     1741 acattgcgcg acgcattttt aaaagttggc ctgcgcgcga tgacctgttt tgaaactact
     1801 gaccgtaaca acggcatcaa agagttgcag gaaggtgtag aagaaaacat ccgtttcgcc
     1861 cgtttgattg atgaggcgaa gaaagcgaca agcgagccgt atctggtgga agcacatatc
     1921 ggtgctcacg cgccgtttac cgtgccggat gccggtctgg agatgctgcg tgaagccgtg
     1981 aaagccacag gccgtggttt gcatattcac gctgcggaag acctttacga cgtttcctac
     2041 agtcaccact ggtacggcaa agacctgctg gcacgactgg cgcaattcga cctcatcgac
     2101 agcaaaacgc tggtcgctca tgggctgtac ttgtcgaaag atgacatcgc cctactcaat
     2161 cagcgcgatg cgttcctggt gcataacgcc cgttcaaaca tgaacaacca tgtcggctac
     2221 aaccatcacc ttagcgacat ccgcaacctg gcattgggaa cggacggcat tggttcggac
     2281 atgtttgaag agatgaaatt tgccttcttt aaacatcgcg atgcgggtgg tccgctgtgg
     2341 cctgacagtt ttgccaaagc cctgactaac ggtaacgaac tgatgagccg caactttggc
     2401 gcgaaatttg ggcttctgga agccggttac aaagctgatt tgaccatttg cgattacaac
     2461 tcgccgacgc cgctgctggc agacaatatc gccgggcata tcgctttcgg tatgggctca
     2521 ggcagcgttc acagcgtgat ggtcaatggt gtgatggtct atgaagaccg tcagtttaac
     2581 ttcgattgcg attccattta tgcacaagcc agaaaagccg ctgccagtat gtggcgtcgg
     2641 atggatgcgc tggcataaat gacagatgcc ctcttcccgc agggaagagg gttaaataag
     2701 gaaagaagat gattgaacaa tttttcaggc ccgactctgt cgaacaggcg ctggaactga
     2761 agcgccgcta ccaggatgaa gccgtctggt tcgccggggg cagcaaactc aatgctacac
     2821 caacccgtac cgataaaaag attgccattt ccttacagga tctggaactg gactgggttg
     2881 actgggataa cggtgcactg cggattggtg caatgtctcg cttgcagcca ctgcgtgatg
     2941 cgcgctttat tcctgcagca ctgcgtgaag ccctcggttt tgtttactca cgccatgttc
     3001 gtaatcagtc gaccattggt ggtgaaatcg ccgcccggca ggaagagtca gtgctgcttc
     3061 ccgtcctgct agcactggat gctgaactgg tttttggcaa cggcgaaacg ctgtcaatcg
     3121 aggactacct ggcctgccca tgcgatcgcc tgctaaccga aattatcatt aaagatccgt
     3181 atcgcacctg tgcgacccgc aaaattagcc gttctcaggc aggtttaacc gtcgtgacgg
     3241 cagccgttgc aatgacagac cacgacggta tgcgaattgc gctggatggc gtcgccagta
     3301 aagcactgcg tctgcacgat gtcgaaaaac aaaatctgga aggcaatgca cttgaacagg
     3361 ctgtcgccaa cgccattttc ccgcaggaag atttgcgggg cagcgtggcc tataaacgct
     3421 atatcacggg agttctggta gccgacctgt atgccgactg ccaacaggct ggggaggaag
     3481 ccgtatgatc atccacttta ctttaaatgg cgcgcctcag gagctaaccg ttaatccagg
     3541 cgaaaacgtg caaaagctgt tgtttaacat gggaatgcac tctgtacgca acagtgatga
     3601 tggcttcggg tttgccggtt ctgacgcaat aatctttaac ggtaatatcg ttaacgcgtc
     3661 cttgcttatt gccgcacagt tagagaaggc agatattcgt accgcagaat ctctgggtaa
     3721 atggaacgag ttaagtctgg ttcaacaggc aatggttgat gttggcgtgg tgcagtctgg
     3781 ttataacgat ccagctgcag ctctgattat caccgatctt ctcgatcgca tcgccgcacc
     3841 tacccgcgaa gagatcgacg acgcgctttc tggtttgttc agccgcgatg caggctggca
     3901 acaatactat caggtcattg aactggcggt tgcacgtaaa aataatccgc aggccaccat
     3961 tgatatcgct ccgactttcc gtgacgacct tgacgtcatt ggcaagcatt atcctaaaac
     4021 tgatgccgcg aaaatggtgc aggcgaaacc ctgctatgtt gaagactgcg taacggctga
     4081 cgcctgcgtc attaaaatgt tacgtagccc acacgctcac gcactgatta ctcatctgga
     4141 tgtcagcaaa gctgaagcct taccgggcgt cgttcacgtt attactcacc tgaattgccc
     4201 tgatatctac tataccccgg ggggtcagag cgcaccggaa ccgtcaccgc ttgaccgccg
     4261 tatgttcggc aagaaaatgc gtcacgtcgg cgatcgtgtt gctgcggtcg tcgcagaaag
     4321 tgaagaaatt gcgctcgaag cactgaagct catcgacgtt gaatatgaag tgcttaagcc
     4381 ggtaatgtcg atcgacgaag caatggcgga agatgcgcct gtcgtgcacg atgaaccggt
     4441 ggtgtatgtt gctggtgcgc cagatactct ggaagacgat aacagccatg cagcccagcg
     4501 cggcgagcat atgatcatca acttcccgat cggttctcgc cctcgcaaaa atatcgccgc
     4561 cagtattcat ggtcatattg gcgatatgga caaaggcttt gccgatgccg atgtgatcat
     4621 tgagcgaacc tataactcaa cgcaggcaca gcagtgcccg actgaaacac atatctgctt
     4681 tacccggatg gacggcgatc gtctggttat ccacgcctcc acccaggtac catggcactt
     4741 acgccgccag gtcgcgcgcc tcgtgggcat gaaacagcat aaagttcatg tcattaaaga
     4801 gcgagttggc ggcggttttg gttccaaaca ggacatcctg ctggaagaag tgtgcgcctg
     4861 ggcaacctgc gtgaccgggc gtccggtact attccgctac acccgtgaag aagagtttat
     4921 tgctaacacc tctcgtcacg tcgcgaaagt caccgtcaaa ctgggagcga aaaaagatgg
     4981 tcgcctgacg gcagtgaaga tggatttccg cgccaacact ggcccttacg gcaaccactc
     5041 actcaccgta ccgtgtaacg gaccggcgct gtcgctgccg ttatatccgt gcgataacgt
     5101 cgatttccag gtcaccacct actacagcaa catttgccca aatggtgctt atcagggtta
     5161 tggcgcaccg aaaggtaact tcgctatcac catggcatta gcggaactgg ctgaacagtt
     5221 acagatcgac caactggaaa ttatcgaacg taaccgggta cacgaagggc aagagctgaa
     5281 aattctcggt gcaatcggtg aaggtaaagc gccgacctcc gttccttccg ccgccagctg
     5341 tgcactggaa gagatcctgc gtcagggtcg cgagatgatc caatggtcgt caccgaaacc
     5401 gcaaaatggt gactggcaca tcggtcgcgg cgtcgccatt atcatgcaga aatcgggtat
     5461 cccggatatc gatcaggcta actgcatgat caaactggaa tcggacggca cctttatcgt
     5521 tcattctggc ggtgcggata ttggtactgg tctggatact gtagtgacga aactggcagc
     5581 agaagtgctg cactgcccac cgcaggacgt gcatgttatc tccggtgata ccgatcatgc
     5641 gttgtttgat aaaggcgcat atgcctcgtc cggtacttgc ttctcgggta acgcggcgcg
     5701 tttggcagcg gaaaatctac gggagaaaat tctgttccac ggcgcgcaaa tgttgggtga
     5761 ggcagtggca gatgttcaac tggcaacgcc gggcgtcgtg cgcggcaaga aaggcgaagt
     5821 tagtttcggg gatattgccc ataaaggcga aaccggcatc ggctttggtt cactggtggg
     5881 aactggcagt tatatcacgc ctgatttcgc cttcccgtat ggcgcaaact tcgctgaagt
     5941 tgccgtcaac acgcgtacgg gtgaaatccg cctggataaa ttctacgcct tgctggactg
     6001 cggtacaccg gtcaacccgg aattagcatt gggacaaatc tacggtgcta ccctgcgagc
     6061 tatcggccac agtatgagcg aagagatcat ttatgacgcc gaaggtcacc cgttaacgcg
     6121 tgatttacgc agttacggcg caccgaaaat tggtgacatt ccgcgtgatt tccgcgctgt
     6181 gctggtgccg agcgacgata aagtcggccc gttcggggcg aaatcgatct cggaaatcgg
     6241 tgtaaatggc gcagctccgg cgattgctac cgcaattcac gatacatgcg gcatctgggc
     6301 agaagaaagt caaagtgggc atgggaacgg atatcggtgc cggaacaact ttcaacatgc
     6361 tgcaaacgct gaacgaagcc tacaaagtgt tgcaattaca aggctatcgc ctctcggcat
     6421 atgaagcgtt ttacctggcc acgctcggcg gagcgaaatc tctgggcctt gacgatttga
     6481 ttggcaactt tttacctggc aaagaggctg atttcgtggt gatggaaccc accgccactc
     6541 cgctacagca gctgcgctat gacaactctg tttctttagt cgacaaattg ttcgtgatga
     6601 tgacgttggg cgatgaccgt tcgatctacc gcacctacgt tgatggtcgt ctggtgtacg
     6661 aacgcaacta ataataaaac tttaacatcc tcgtgaggac atcattatgt ctggagacat
     6721 cctacaaaca ccggacgcac caaagccaca gggcgcgctg gataattatt ttaaaattac
     6781 cgctcgtggc agtaccgttc gtcaggaagt actggctggc ttaacgacct ttctggccat
     6841 ggtttattcc gttatcgtcg ttccgggaat gctgggcaaa gcagtttttc ctcccgcagc
     6901 tgtgtttgtt gccacctgtc tggtcgcggg cttcggctcg ttgctgatgg ggttgtaggc
     6961 caatttgcca atggcgattg gttgtgcgat ttccttgacg gcatttaccg cattcagtct
     7021 ggtactcggg caacaaatta gcgttcctgt cgcactggtc gcattttacg cgggtggttg
     7081 ggcaatctaa tagattctcc gcgcctttca gcgcggagat ttctttcaca gaggattctc
     7141 accgctggca acaatacgct gctggcggac tcgcctgagt tctctttcag taagcaactg
     7201 cagcgcctgt gtcgggcaag attcaacaca ggctggcccc tcttcccgct gttcacagag
     7261 atcgcatttc acaatttgtt gtcgggtatc ccccggcaac gactgaatgg tgatcattcc
     7321 aaacgggcac gcactgacgc aactctgaca gccaatacaa cgggcagaat tggtcggcac
     7381 cacctgctcg cccatcgtca gcgcccccac ggggcaagcg ccaacacaag gggcgttttc
     7441 acactgatgg cacatcaccg gcgcactgat gctgtccagt cgctgtacct tcagccgggg
     7501 taaaaagaca tcggcattca actcctgttc tgaagggtga gcgaccacac aggctacttc
     7561 acaagtacgg cagccaatac agtcagccgg attaacgata attaacgatt tcatcacgat
     7621 gccttcgtat caaacagagt taacatatcg cgcgccgcct gccttcctgc ggccattgca
     7681 gtgacaacca gatccgcgcc atgaactgca tcaccaccag caaagacttt tttcagatgc
     7741 gtctgggtag gtaaataccc gacgtcaccg gtttgaatca ggccccattt atcgagttta
     7801 attccgctgc cctgcaacca cggcatggca tgcgcctgga aaccaaaggc cataatcaga
     7861 acatcggcgg gcaattcaaa ctctgaaccc gctaccggac gaggacgacg gcgaccatcc
     7921 ggccccggct cacccatggc ggtacgaatc aggcccaccg cagttaaacg cccatcttcg
     7981 tcacaagcga tatattgcgg ttgaacattg aactgaaact cgacaccttc ctcgcgcgca
     8041 ttaaccacct ctttgcgcga gcccggcata ctgacttcat cacgacgata cgcgcaggtc
     8101 acgctggcag cattgaggcg aatggaagtc cgcaaacaat ccattgtcgt atcgccaccg
     8161 cccaatacca cgactcgctt accttccacg tccgtcagcg gatactcttc agactccggc
     8221 aatcccatga gctggcgggt atgggcagtc aggaacggta gagcctgaat gacaccgggc
     8281 gcatcttcat gcggcagatc tgctcgcatc atcccgtaag tccccacgcc gatgaaaact
     8341 gcatcatatt cagaagttaa atcgctaaag gtaatatcgc ggccaatttc acagttaaga
     8401 tgaaagtcga ttcccattgc ggtgaatatc tctcgccgct ggcttaatac cgttttatcg
     8461 agtttgaaag gaggaatgcc aaaagtcagc ataccgccaa tttctggatg gcgatcaaag
     8521 acatcaacct gaactcccgc gcgcgccaga atatcagcac accctaaccc tgctggccca
     8581 gcgccaatca ccgccacttt ttcgatacgg ggaacaactt tgctgacatc gggacgccag
     8641 cccatcgcca gcgcggtatc ggtgatgtag cgttccagat taccgataga gactgcgcca
     8701 gagtgatctt tcaaagtaca tgcgccttca caaagacggt cctgtgggca taccctgccg
     8761 cagatttctg gtaaggaact ggtctggtgg caaagttctg ccgcttcaat aatctttcct
     8821 tcctgtacca gacggatgta atccggaata gcgttatgca gcgggcaatg ccagttgcag
     8881 ttagcttttt cggcacaata aacacagcgg tcactctcat aagtcgcttg cagtggatcc
     8941 agcccgcaat agatttcgcc aaagtgggtt ttccgttcac tcgctgaaat tttatctgcg
     9001 cctttacgcg agttaacggg gagcaacgct gcactgcgag atggctgagc gtctgatgac
     9061 gcttttcctg ctgccgtttt gcgctggcga gccaccttta tccactgtaa ccctttatcg
     9121 tccatcagtc gtaacgcctg tgttgggcag acttcaatac aggcttgcgt gccggaactg
     9181 cgctggttac aaaggtcgca tttctgcgca atcgtatcga ccatctcaac gacgccaaag
     9241 gggcaagcga ttgcgcatct tttacagcca atacattttt gctcgtccag ttgtacgcta
     9301 tcggactgga aagtcagagc attaaccgga caagccgtaa cacaaggggc attgttgcag
     9361 tgatggcagg ccaccggatt cgcagcctgg cctttcccta caacgtggat acgcggtcga
     9421 aagtcactgt gactcagcgg ccagttttct tgattgtgtg ccaccgcaca ggcaatttca
     9481 caagcatgac agcctataca ttccgcagct tcagcagcga taaacttatt catttgcatc
     9541 cctttcat
//
LOCUS       DAJVXP010000145         9432 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVXP010000145 DAJVXP010000000
VERSION     DAJVXP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.145"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="OQS10_003465"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="OQS10_003465"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS2294408.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="OQS10_003466"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="OQS10_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS2294409.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="OQS10_003467"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="OQS10_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OQS10_003468"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OQS10_003468"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS2294410.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="OQS10_003469"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="OQS10_003469"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS2294411.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="OQS10_003470"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="OQS10_003470"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS2294412.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGCRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="OQS10_003471"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="OQS10_003471"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS2294413.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="OQS10_003472"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="OQS10_003472"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS2294414.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="OQS10_003473"
     CDS             complement(6556..7449)
                     /locus_tag="OQS10_003473"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS2294415.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="OQS10_003474"
     CDS             complement(7446..8099)
                     /locus_tag="OQS10_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS2294416.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="OQS10_003475"
     CDS             complement(8110..9369)
                     /locus_tag="OQS10_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS2294417.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2672 c         2498 g         2160 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggctgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAJVXP010000146         9397 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVXP010000146 DAJVXP010000000
VERSION     DAJVXP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.146"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(350..1603)
                     /locus_tag="OQS10_003476"
     CDS             complement(350..1603)
                     /locus_tag="OQS10_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2294418.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OQS10_003477"
     CDS             1710..2603
                     /locus_tag="OQS10_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2294419.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OQS10_003478"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OQS10_003478"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS2294420.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OQS10_003479"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OQS10_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS2294421.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OQS10_003480"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OQS10_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS2294422.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OQS10_003481"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OQS10_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS2294423.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OQS10_003482"
     CDS             complement(6839..7693)
                     /locus_tag="OQS10_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS2294424.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OQS10_003483"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OQS10_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS2294425.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="OQS10_003484"
     CDS             complement(8323..>9397)
                     /locus_tag="OQS10_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS2294426.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2378 c         2438 g         2132 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagta gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAJVXP010000147         9213 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVXP010000147 DAJVXP010000000
VERSION     DAJVXP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.147"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            74..733
                     /locus_tag="OQS10_003485"
     CDS             74..733
                     /locus_tag="OQS10_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS2294427.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            837..1811
                     /gene="ghrB"
                     /locus_tag="OQS10_003486"
     CDS             837..1811
                     /gene="ghrB"
                     /locus_tag="OQS10_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS2294428.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1861..2571)
                     /locus_tag="OQS10_003487"
     CDS             complement(1861..2571)
                     /locus_tag="OQS10_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS2294429.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3005..3295
                     /locus_tag="OQS10_003488"
     CDS             3005..3295
                     /locus_tag="OQS10_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS2294430.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3576..3788
                     /gene="cspA"
                     /locus_tag="OQS10_003489"
     CDS             3576..3788
                     /gene="cspA"
                     /locus_tag="OQS10_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS2294431.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="OQS10_003490"
     CDS             complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="OQS10_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS2294432.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="OQS10_003491"
     CDS             complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="OQS10_003491"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS2294433.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="OQS10_003492"
     CDS             complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="OQS10_003492"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS2294434.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7539..7838)
                     /locus_tag="OQS10_003493"
     CDS             complement(7539..7838)
                     /locus_tag="OQS10_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS2294435.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8013..9008
                     /gene="wecH"
                     /locus_tag="OQS10_003494"
     CDS             8013..9008
                     /gene="wecH"
                     /locus_tag="OQS10_003494"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS2294436.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9050..>9213)
                     /locus_tag="OQS10_003495"
     CDS             complement(9050..>9213)
                     /locus_tag="OQS10_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294437.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2331 t
ORIGIN      
        1 ttcatgttca cgcggcataa tctcccgcca cggaacccgt ggcaaagaat aaaaaggtta
       61 ttaaggatta acaatgaaga aacgtgttta tcttattgcc gccgtagtga gtggcgctct
      121 ggcggtatct ggctgcacaa ctaaccctta caccggcgaa cgcgaagcag gcaaatctgc
      181 tatcggcgca ggtctgggct ctctcgtggg cgcgggtatt ggtgcgctct cttcttcgaa
      241 gaaagatcgc ggtaaaggcg cgctgattgg cgcagcagca ggcgcagctc tgggcggcgg
      301 cgtgggttat tacatggatg tgcaggaagc gaagctgcgt gacaaaatgc gcggcactgg
      361 tgttagcgta acccgcagcg gggataacat tatcctcaat atgccgaaca atgtgacctt
      421 cgacagcagc agcgcgaccc tgaaaccggc aggcgctaac accctgaccg gcgtggcaat
      481 ggtgctgaca gagtatccga aaacggcggt taacgtgatt ggttataccg acagcacggg
      541 gggtcacgac ctgaacatgc gtctctccca gcagcgtgcg gattccgttg ccagcgcgtt
      601 gatcacccag ggcgtggacg ccagccgcat ccgtactcag ggccttggcc cggctaaccc
      661 aatcgccagc aacagcaccg cagaaggtaa ggcgcaaaac cgccgtgtag aaattacctt
      721 aagcccgctg taatcccttt catgccaggt gatgcagaaa tcacctggca ttcttcagtt
      781 ttccttcatc atatttcagg ctaaggtgat cgccttatca gtgaatggag agaagcatga
      841 agccgtccgt tatcctctac aaagccttac ctgatgattt actgcaacgc ctgcaagagc
      901 atttcaccgt tcaccaggtg gcaaacctca gcccacaaac cgtcgaacaa aatgcagcaa
      961 tttttgccga agctgaaggt ttactgggtt caaacgagaa tgttgatgcc gcattgctgg
     1021 aaaaaatgcc gaaactgcat gccacatcaa cgatctccgt cggctatgac aattttgatg
     1081 tcgatgcgct taccgcccga aaaattctgc tgatgcacac gccaaccgta ttaacagaaa
     1141 ccgtcgccga tacgctgatg gcgctggtgt tgtctaccgc tcgtcgggtt gtggaggtag
     1201 cagaacgggt aaaagcaggc gaatggaccg cgagcatagg cccggactgg tacggcactg
     1261 acgttcacca taaaacactg ggcattgtcg ggatgggacg gatcggtatg gcgctggcac
     1321 aacgtgcgca ctttggcttc aacatgccca tcctctataa cgcgcgccgc caccataaag
     1381 aagcagaaga acgcttcaac gcccgctact gcgatttgga tacactgtta caagagtcag
     1441 atttcgtttg cctgatcctg ccgttaactg atgagacgca tcatctgttt ggcgcagaac
     1501 aattcgccaa aatgaaatcc tccgccattt tcattaatgc cggacgtggc ccggtggttg
     1561 acgaaaatgc actgatcgca gcattacaga aaggggaaat tcacgccgcc gggcttgatg
     1621 tcttcgaaca agagccactg tccgtagatt cgccgttgct ctcaatggcc aacgtcgtcg
     1681 cagtaccgca tattggatct gccacccatg agacgcgtta tggcatggcc gcctgtgccg
     1741 tggataattt aattgatgcg ttacaaggaa aggttgagaa gaactgtgtg aatcctcacg
     1801 tcgcggacta agccgcgact gcgtgaagta aagcccgata atcgctcggg cttttactct
     1861 ttattgggtt gcagtaactg ctgtagtcca ggcctgatta aacgcctgat gttgtgccgg
     1921 taatggcgca atcagtttgt tatattcact tgcctgctgt gaagtcggga actggatgcc
     1981 atttgcgaca aagctcacct gagtaccttg ctgagcaatg tagtctccaa ccatcaccag
     2041 ttgttgcgtg aaagtttgtg ccgccggaat caacggttgc aatgcatcag ctggcgtcgt
     2101 taccactttg gtgaatgcct gatcaaatac cggtttcagg tcatcgctct gttttagcgc
     2161 agagtgcgca gcatcggcct gcaacttcgc attttgcagt tgttgcgcca gcacacccag
     2221 cgaaccattc atttcacgca gcggaccgct ttgcgtaacg taatcctgcg gtacgcgaat
     2281 tgcgttaacg ctgtctacga ccgggcgcag accagaatcc atcgcctgat tcacctgctg
     2341 agaataacca tacagaatcg cgtaatcaga gacaaaagga ccaaactgtt ttttctgatc
     2401 ggcagtcagg gttggtagac gttcaccgct acgcatcact gtattctgca ggaagtcgat
     2461 aaacgcttta cgctgatcgc cttctttatc aaaacaccca ctcaggctaa ctaccattaa
     2521 taacgccgca agaggcgcaa accagcgaga gcaggacttt cctgtcgcca ttttcttact
     2581 cctttcaccc aaaaaaagcg cacaacgaca cacgcgtgtc tgacgtgaca aggatagtcc
     2641 aggacaggct tgcaggatac cctttctacg taatcttcct ggtaaaatca tggagaaaat
     2701 gaaacaaggt taatttgttg tttcattgtt aaaaaatgat agtgccgttg ccttttttgt
     2761 agccagagat ttacgccttc gcagaaggtt agattttcga aatctttaca aaacaggagt
     2821 ggtaagagat atatcggcat ttatcagatc gttaggtggc tataagtcac gctaaatgac
     2881 aggctgaatc gaatcatagc cagagcatgc cctgacttca ccccgctgtg tctgcttttc
     2941 ccgactattc ttaatgagct tcgatgcaat tcacgatccc gcagtgtgat ttgaggagtt
     3001 ttcaatggaa tataaagatc caatgcatga gctgttgagc agcctggaac agattgtttt
     3061 taaagatgaa acgcagaaaa ttaccctgac gcacagaaca acgtcctgta ccgaaattga
     3121 gcagttacga aaagggacag ggttaaaaat cgatgatttc gcccgggttt tgggcgtatc
     3181 agtcgccatg gtaaaggaat gggaatccag acgcgtgaag ccttcaagtg ccgaactaaa
     3241 attgatgcgt ttgattcaag ccaacccggc attaagtaag cagttgatgg aatagacttt
     3301 tatccacttt attgctgttt acggtcctga tgacaggacc gttttccaac cgattaatca
     3361 taaatatgaa aaataattgt tgcatcaccc gccaatgcgt ggcttaatgc acatcaacgg
     3421 tttgacgtac agaccattaa agcagtgtag taaggcaagt cccttcaaga gttatcgttg
     3481 atacccctcg tagtgcacat tcctttaacg cttcaaaatc tgtaaagcac gccatatcgc
     3541 cgaaaggcac acttaattat taaaggtaat acactatgtc cggtaaaatg actggtatcg
     3601 taaaatggtt caacgctgac aaaggcttcg gcttcatcac tcctgacgat ggctctaaag
     3661 atgtgttcgt acacttctct gctatccaga acgatggtta caaatctctg gacgaaggtc
     3721 agaaagtgtc cttcaccatc gaaagcggcg ctaaaggccc ggcagctggt aacgtaacca
     3781 gcctgtaatc tctgcttaaa agcacagaat ctaagatccc tgccatttgg cggggatttt
     3841 tttatttgtt tttcaggaaa taaataatcg atcgcgtaat aaaatttagt attatttttg
     3901 tgaagaataa atttgggtgc aatgagaatg cgcaacgccg taagaaaggc gggaataact
     3961 tcccgccaaa gactcttact ctttcaaatt gcaggctaaa aatgccgcca gctcataact
     4021 ctcctgttta atatgcaatt cacacagtga atctcttatc atccaggtga aaaataaaag
     4081 cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc ccagcctcct
     4141 gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag ggtagcctca
     4201 ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat acctgagaca
     4261 aacagtctca agcacccgtg gctattctag cttaataagt ttgtttcttc tccttgatat
     4321 aatcctaaaa aaacctcata aaattaatat atgagataat ctttattcaa cagaagatta
     4381 ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt
     4441 aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca
     4501 gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca
     4561 ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct
     4621 ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg
     4681 ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt
     4741 cgtcagattt cgccagaatg ttagatacac gtttgttcgc cgccgccagt gcagcagctg
     4801 catccagggt acggaaatgc gataccgctt tcatacgggc atcgaaatca gccggacgag
     4861 tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc
     4921 aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg gcgttagtca
     4981 gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca aggttgaggt
     5041 tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt
     5101 tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc atcttatcag
     5161 caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca aagcgcggct
     5221 gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga cgcgcgtagt
     5281 gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc atcaggtcac
     5341 acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa
     5401 tccagccagc cagcgcctgg atgcggtcag ttttgtcacg cagcgtaccc agctgttgct
     5461 ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat
     5521 tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa
     5581 tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc
     5641 catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt
     5701 cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca
     5761 ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac
     5821 gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga
     5881 ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg
     5941 gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg
     6001 ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca
     6061 cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccagc gaagtcgcaa
     6121 ccatattcgg cagtagtgct tcagtgcttt cgcccttcac atgggcgcga tacagcagcc
     6181 attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg
     6241 cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg
     6301 ggccgcgttt ttcgatttca cgatctggtt gcgcttccgc caggttagct actttcaacg
     6361 ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca
     6421 gctccgcagt aaagttcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca
     6481 gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat
     6541 ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg
     6601 ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcagtgacg gagatggctt
     6661 tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca
     6721 gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcgaagc
     6781 aggtgaacag gaaatccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt
     6841 tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt
     6901 cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct
     6961 cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca
     7021 cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt
     7081 cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca
     7141 gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact
     7201 gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag
     7261 aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg
     7321 ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc
     7381 gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt
     7441 gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg
     7501 atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa
     7561 aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc
     7621 atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg
     7681 ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga
     7741 tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaccaacc cactagcacc
     7801 ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag
     7861 gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc
     7921 tataagctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt
     7981 cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata
     8041 acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga
     8101 ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct
     8161 ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca
     8221 gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg
     8281 cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc
     8341 tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg
     8401 tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg
     8461 tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc
     8521 tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg
     8581 gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc
     8641 tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg
     8701 gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg
     8761 cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa
     8821 tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca
     8881 cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga
     8941 cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag
     9001 tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt
     9061 attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca cgaatattct
     9121 tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt
     9181 cactgagtaa taatgtcgca ttccacagac cta
//
LOCUS       DAJVXP010000148         9207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVXP010000148 DAJVXP010000000
VERSION     DAJVXP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.148"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..988)
                     /locus_tag="OQS10_003496"
     CDS             complement(<1..988)
                     /locus_tag="OQS10_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCS2294438.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPE"
     gene            complement(985..2781)
                     /locus_tag="OQS10_003497"
     CDS             complement(985..2781)
                     /locus_tag="OQS10_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS2294439.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3032..3388)
                     /locus_tag="OQS10_003498"
     CDS             complement(3032..3388)
                     /locus_tag="OQS10_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294440.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3446..3868)
                     /locus_tag="OQS10_003499"
     CDS             complement(3446..3868)
                     /locus_tag="OQS10_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS2294441.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(3884..4609)
                     /locus_tag="OQS10_003500"
     CDS             complement(3884..4609)
                     /locus_tag="OQS10_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCS2294442.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4631..5377)
                     /locus_tag="OQS10_003501"
     CDS             complement(4631..5377)
                     /locus_tag="OQS10_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2294443.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5384..6448)
                     /locus_tag="OQS10_003502"
     CDS             complement(5384..6448)
                     /locus_tag="OQS10_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS2294444.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6520..6945)
                     /locus_tag="OQS10_003503"
     CDS             complement(6520..6945)
                     /locus_tag="OQS10_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS2294445.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(6942..7205)
                     /locus_tag="OQS10_003504"
     CDS             complement(6942..7205)
                     /locus_tag="OQS10_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS2294446.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7317..7817
                     /locus_tag="OQS10_003505"
     CDS             7317..7817
                     /locus_tag="OQS10_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2294447.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            7837..8031
                     /locus_tag="OQS10_003506"
     CDS             7837..8031
                     /locus_tag="OQS10_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS2294448.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8031..8321
                     /locus_tag="OQS10_003507"
     CDS             8031..8321
                     /locus_tag="OQS10_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS2294449.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8601..8753
                     /locus_tag="OQS10_003508"
     CDS             8601..8753
                     /locus_tag="OQS10_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS2294450.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8753..9130
                     /locus_tag="OQS10_003509"
     CDS             8753..9130
                     /locus_tag="OQS10_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294451.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2361 a         2420 c         1957 g         2469 t
ORIGIN      
        1 attctggtat tgccttttta aaaggcagag cacttctgtc cataaatgcc gtaaggaatg
       61 caagattttt ctcgctgaac ccagcgtcaa tagccaaatg agtgagagta acttttcgtt
      121 ccctgtttat aggcccatct gtcgcaacta cttcagtgaa aactacaagc atgtccgaac
      181 ccgttcggtc ctctcccaaa tcaatcaaaa taatatctgg aagtgctttt gaggcatcaa
      241 tattcagccc caatgctttt gccaatacat catcctgagc aacaacttta ttcccggatt
      301 cagacaacca gagcacagtt ggattcttaa gaaaattagg agcaaaaact tcgatgacgg
      361 ccttagctat tgtacttgac gggcccggag ctaaagttct cttctctcca tttgggaaag
      421 tgacgactac ggcatcagca gcaatcactg cccctgattt caataaacgc aacctagcaa
      481 gagcggcttt gttcaaatga ttttcttgcc aggaacttat tgcactcagc agcgaatctc
      541 cttcaagccc ggcatcaaag agtgctgcaa aagagcgatt caaactgtaa cgaggacgag
      601 aggatgtggt gggtatacct ttacgctcaa caattgcatt gcaaggtatt aggccatttt
      661 ttatagtttc atcacgtatt ggctcacgcg tattttccgc ataccatgca tcagcaggcc
      721 ttatcttttg tttagagaaa gtcatcttta cccagctttc tctggacgca tcatcagtaa
      781 gagcagcctg cgaatctccc atacttacaa cctggctggg ccttatccat cgccccagcc
      841 cctcaaccgc accagcataa aacatgacaa aaattgtttt tgcagctatt tcccgaatta
      901 aatatccacg attctcagtc ccttcaggga aaattaattg taatctttcc cggataacat
      961 tcaatggggg aacaacaggc agattcatgc aatcatcact ccatagaaac cagcgatagt
     1021 tctctcaata atccgtctgt ggacgccagc atcaattaac gattgaatct ccatcacctg
     1081 ctccagagaa ggcaacggga ttgaattcaa ttcataggcc gaaacagcaa cacttccgct
     1141 tatacacctg aacgcccgat cgacaacatg cgaattaagt agcatatcaa taacatcagg
     1201 gcgaaccgca gagaacaatc cgtttgaata tacaatatta atatggtttt caactactac
     1261 gccaccagtt tcatctataa atccctgagg taaaattgcc gctaaaatcc ttctgtcctg
     1321 ttccttcgat gttgtccgct gaacaagcac acattcagat tttgtcacaa ggaaaccctg
     1381 ttgtggggtt atatcgatat agggaacatg attttttctg tctgcactaa aacgaaaacc
     1441 agctgaagtt attgattccg cccagactaa tgggtagcta ttttttcctt tcgtcgtacg
     1501 caactgcgac ttaaatcgat tccagactaa ctgacctgta gagactgagt atcccagatc
     1561 agctaaacgc gtagacatct gttttaatac attaagaaaa caggcatcat ctttaacgcg
     1621 tggcaaaagc cacgttgctc cacctttttc tatatcaaca ttacctattt tctcaatttt
     1681 tgctttgttc aaccccttag ggacaagtga ggatacctgg gcccgaactt tatgttctcc
     1741 cgccttgaat gctgtcaaca ttgtttcctg gagaacatcg tcaaagaccc catccctgtc
     1801 agtgacgaaa tcaagggcat acggagtagt tttttcggtc aaaagggttc gcagcgcggt
     1861 gaaatactgc ccaccaagaa aagaagttgg cgtaagaaat gcaataactc ctgttttctc
     1921 tttaaccatg cgaacagcta agtcggtgaa caatccatat agatttgcat gaccaaataa
     1981 cgaacgagaa tatttttctc tcgttttaat atcgagagtg acacgtccgt aaggcggatt
     2041 ccctaccaca agatcataac cagaaaattt atcttgctga agcgcatcag caacaataat
     2101 tgtatcttca ggcaatctac gttttacctt tacacatagt ggcattagta cagattcgag
     2161 caataccaaa ctcatccaag ccgcaaatgg atcgatctct atccccttta aacgacgact
     2221 gattctcctc aacatccatt caggggaaga ccccttgtct ttttttaaca ttcgaatggc
     2281 tactggtgcc aaaaatgcac cgccaccaca cgcaggatca ataaccgatg catgagaaaa
     2341 atcaacacca gacttttcag ccaggtccaa gagacgcgct accaaaggtg ggggggtata
     2401 gtaagccccc aattccgaac ggtaagcgga tggcagcatt accgtataaa ttgacccaat
     2461 cagatatcca gcgtcctccg ccggaaattg agcaataagc tcacctgttc gttcagcgag
     2521 ctcgcgtgca tcacatggaa tgtcttctat gtaaacagtg tgcggtactg ccctcatttt
     2581 aatgctgctt ttttgctttt ttgcaagggt atcccaatat gcagctacga tgctgaagca
     2641 gtatgagcgg gcatgcatca accttgactg cccggttttg taattgcccg cataaccttt
     2701 ggccatggct ttacaagttt gatatctttc cagattagaa aactcattat ctgtaaaaaa
     2761 atcaaaatgt tgcacattca taatgtaaaa tcctaatgta tcaaacatgc gggccataac
     2821 atctcatggt ggcgcatagc aaactggccc gaagaatatc acgcaccaag tcgatctcat
     2881 aacccgttat gacaataaat ctttgtagct gaactcactt ggtgatttgt caatcattcc
     2941 tggcctccag ttcgtcctga atctctttat cgagttcacc gtatttgtta gtcctcatca
     3001 ctcacgactt gacttccaga aataaccttg attattcggg gctatatttt gtcaccctgc
     3061 gtatccgcgc tttcgcatta cgctcaatct ggattagctt ttctatattt tttcgccttt
     3121 cccgctcctc ctggcgcaag agccttacat catctgccag tctggtttct cttttcgcca
     3181 cagagagcat ccagtcaaat ggctccacaa ctgcaccgca aattttacag cggacctgac
     3241 gctctttttc gtcaacccgg acagaagcgt gatggcaata tggtctttcc gatggctcat
     3301 aaagaaaatt aacctgatta cgagggttat cctcttttac cggaaataaa acaatattgc
     3361 ttaactcatc ttctggtttt atttccatgc tcctctcctt tgatgcgaat gccagcggta
     3421 attgaagcct gatagctaat ttcactcaca gtaccgcctc ctgaaaatca ccctgataga
     3481 aagccggtac acgctgcata acttcactct tccggcactc gcgacagatt atgttctgat
     3541 gtctgtcgta gcggcgtatt tctccgtccg gtaatgacca gataaggtcc ggatcaacca
     3601 cagatggttt cttcagcttt gccctcgaga gttttttgcg ggcgttttgc cagtccttac
     3661 gagcctgttc agacgggaat aacccgtagc cagagttgta tacatcccca ctggcaacca
     3721 gctctctggc gagaacgctt attaaatatc ttgtcgcccc ggttttagct tccagttgtc
     3781 gtaacgtctc gcgcccactc tggcgtacga gttcaacaac ctgcccctta attttttccc
     3841 gctcttcctg tgtaaatact tttgccataa gcgcccccgg caatcacttt tccgacacaa
     3901 tacgaccgga ggaatcgaca atctgtcgaa caatatcccg gtgcttgttc agctcccgca
     3961 acgcggcgca gacacgctcc cacttctgga catgactttt cgcccgacgc agttcgcggt
     4021 ttgccatatg cagcgatggt aaaaccaggt catccgcttg cgtttcggtg aacgatggca
     4081 acgactgcac aatgtccgcc acagtttctg ttttaatttc ttcctgtgtt gcagcttcct
     4141 gtaccggtaa cgcaacacct gctggctgag gaaagacttt accatccgtt tccgctatgg
     4201 atgcggcttt cggctctgcc ggtaaatcag cgcccggtat gcagtaccga aatttaccgc
     4261 cctgattcac gcgaatcaga cgccctttgc tgattgccat ggccagcgat gaattcgccc
     4321 ggcgggaggt aatcccgaac atcagtgcca gctcatccgc cgtttgtggg ccatgttgtt
     4381 caatcgcctc agtcagcatt tgcgctgtca ctttcggtac cggtgacact ggttcacttt
     4441 caccagcctg aatcagccac cacatcgaac ccttgttatc cgcttcacca cggcgcttca
     4501 gtttccacag ttcgttgacc gcatcttcac ggctgattcc aaggcgggac gccaccacat
     4561 gtgaagaggc tcttttcagt gctttcagtg cgtcaaatac agtttccatt aaaacatcct
     4621 ccaacaaaaa ttacttcaca actcccggaa agctgacatt tgaacgccag ctatcccagt
     4681 taaacgtcac ccaccgacaa ccgttcatgg tcatgcggtc cataatcctc tcaccaagaa
     4741 gcgtgctcat tgcggcatga ttcaggtttg ttaacatccc gacactgcac agtgatgctg
     4801 tccggcgatc aattatctgg tgcaatacca cctgctcgtt tttcgtctcc cgctgaatgc
     4861 ctatttcatc caggaccagc aaatcaaccc cgcaaagctc ctgtaaaaat ttttcaccgg
     4921 atttgccgtt gtcgtagctg tcatgcaaca cgctcatgac atcagactcg gtgacgataa
     4981 tcacgctgcg ccccttcgcc atcagccggt tacccatcgc cgctgcaagg tgatttttcc
     5041 cggtgccggt tttaccgctg aacacaaaat tcgtgcaccc ggtcatcagt tcgtcagcta
     5101 tggatttggc ctggctcagc gcgtattttt gcccgtcgtt ctgcacctga taattcgcaa
     5161 acgagcattt gctgtgcaga ggctggatgc ccgaacgatt caggattttt tccacccgca
     5221 actggcgatt ctggcggtta atctcctcgc tgcgttttcg tccttcagca agttgccatt
     5281 cccgccactc ctccaccgtc cggtacggtg gaaccgcccc ctgtggtgca agtctgtgaa
     5341 tacgttcaag aaccccggct gccgcaatgt ttttcatgcc acatcacccc ctgaatcccg
     5401 gcggaatttc ggtatccggt tcagaaatat gattcacaca acgctggttg ttcgtgccgc
     5461 ttaccgggag caaccagggg ttttcaaaat tccggtccgg cccaaaaaac gtcgtcgctc
     5521 gctgaacaaa ttccgttccc gctttcccgg tcgccgccag gtatctcgcg taacgcctca
     5581 caccatccag catggtctct ggtggcaccc cctcgcgcaa tctggccttc caggcactga
     5641 atgcggattt cttcgggttt gccccggcac gcaacgggta ttcccgccag acctgttcga
     5701 acacatccgg ataatccact cgtcccacag gctgcccggt gttttccggg gctacccgat
     5761 cggcttcccg ctgaatggcg gaatcggctt caggctgctg cagttggagt gattgctccg
     5821 gccttgcggt catcacctgc tgcacagcgc ccgaatcggc tttcagcgca tacgctgaat
     5881 cggcttccgg tgtcgagcct gctggctgac caggattgac ggtctgaaca tcccctgcct
     5941 ggttcgtggc gttttttacg ccatggacca tagtgttttg atcttcttta tctgtatctt
     6001 tatctgtatc tgtcgtgact cgtcgtgaca tgtgcgtgac atttcgtgac gcgccgtgac
     6061 aatcgccatt ttgttcccgc tttctttccc tctctcgctg cgccctcttg cgctctgcag
     6121 gagattttgc ggtttgcgaa atattgccgt tgtcctcttt aagcacctgg cgtttttccc
     6181 atccagtgat taaatcacca tcaagtaccc gcccctgcat cgtctgcaaa attgaatcaa
     6241 ttacctcttc tgtcacgtcg agcgcacttg ccaaatcttc tgtcgtgaca tcaatgtgac
     6301 ctcgcgtgac atttcgtgac gcgctcacca ggaggtggat atacactgcc atcactgttg
     6361 caattggctg ccctgacacc ctggcaattg ttcgccactt agggtcattt ggcatgtcat
     6421 gccataatct gagccaggcg ttagccatac tcacctcttc tgataccgaa tctttttact
     6481 cacgagttgc cgggagcgat tcgatatggc tattgtcagt caatgtactg ccacagcatt
     6541 tcctgccggg ccaccacggt tcatctgatt gaaaccggcg attgccactg cgacaaaatc
     6601 atcagcgtct ctcaccagtc gttcatgcgt ctccaccagt tcccgaaaat aagctgaact
     6661 gtggctgcgc attcgagcca ccagcggagg tggcattgcc ctttcgatcg caggtaacaa
     6721 cgcctgaatt ttttcaacag cgtcaggggt gtctttatcc agccaacgga aaatcttttg
     6781 ggtgttgaga taaagcgtgt tgtgcttgct gtcgttatgc agaaccggaa acgtcattcc
     6841 cagttcgaaa taaaccctgg ttatttcagc tgccggaact ttttcaccgt ccggatgcgc
     6901 ccaggctttc atcgccatgc ggatgtgttc atgcttgatt ttcatgaatc aacccccgcc
     6961 gatggttgtg tgttagcctg aaactcagca ggcaagccgt cagttgggtt ggggtaggtt
     7021 ttactgtcaa tctcatgtgg ggtcaccacc catcccgttg cttcacacca acgtaatatt
     7081 ttttctcctg taagttttgc acgccccgtt ataacgtggc tgaccatccc ttgggatgcg
     7141 ccaacaatct cagcaaatga actctgggtt atatgaaaat gatgcaaata ttctccaaga
     7201 ttcataatcc gccctatgtg atgtgttacc aatgcataat taatagcact gttattttta
     7261 aaagtaaata gtgatactat ttcaaagtga ttagcaatct tattagaatt ggatgtatga
     7321 aaagaaaatc attgtcagac accgacctca acgccgccag aagactgaaa gaaatctgga
     7381 tggcaaagaa atctcagttg ggattgactc aggaacgtgc ggcggaaatt atggggtttt
     7441 caacacaagg ggcggtaagt cactacctaa atggtcaaac cccactaaac cttgaggctg
     7501 ttctaaagtt tgctggctta ctgaaagttc cccctgagtc aatcaggcca gacatggtag
     7561 atttgctgca aattgcaagg atgtattgtc gagaaccaca gcaagataac atagtcgcat
     7621 taccagcaga cacagaacaa acagaggatg aactaccgtt tgccgtagag ccgatggaac
     7681 gagatttaat ccatacgttc agagcttttc cgaaagaaga tcaagaacac atgcttcagg
     7741 agatgaaaga taaaaaagaa tcaatggatc gtacggttgc gcggtggtta gctgcacaaa
     7801 aaggccgtcg cgcctaaatc agaggagata tgtaaaatga gtacagtcct ttccccaatg
     7861 gtttcagaat ttgagacagt tgagcaagaa aatagttaca acgaatggct gcgggccaaa
     7921 gtagcagcaa gcctcgcgga tccccgcccg gcgattccgc atgatgaggt aatggcggaa
     7981 atggaacaaa ttattgccca aattgctgct gcaactcaca gaggtgaata atgttacccg
     8041 ttttatggct accatctgct cgtgatgatt tgcgccagat cgtagcctac atagctaaag
     8101 aaaatcccgc ggcagcacgc agaatgaaaa ttcgaattga aacatccgtt ttgccgctca
     8161 cagagcatcc ttacctgtat cctccaagcg agcgagttct tggtcttcgt gaaattgtaa
     8221 ctcatcctaa ctacatcatc ctttaccgag taacagcatc aagcatagaa gtagtaaata
     8281 ttgtgcattc tcgaagacag tatccaggca aaaacagtta attattcccg ccgacagaac
     8341 caccttaggg tggttttttc ttgccccaac aaaaaacaat agcgatgcta tttacatcaa
     8401 aaaaaataat gatgctattg tttcaggcgt caacccaccc cgccccacag aatgcagggc
     8461 aatacttcga gttaccagac agtggtcagg ggttaagtag ccagcccgag gcgtaagaac
     8521 atgacggcag ggttcaatct ttaactatgc agcaggtttt ttgttccgcc accccggcgt
     8581 taaggggaaa tgaggtcagc atggatacta tcgatcttgg caacaacgaa tctctggtgt
     8641 gcggcgtgtt ccctaaccag gatggaacgt ttaccgccat gacgtatacc aaaagcaaaa
     8701 cgtttaaaac cgaatctggc gcgcgttgct ggttagccag aaacactgac tgatgaggct
     8761 gacgatggaa tttaaagatt taccgtctga tgtacagaaa acagcagctc atacattgca
     8821 ttctgtgctg cgagaaatcg ggaaagatat tgcaagcgag ccagcaaaag atctggcccg
     8881 gaaaatcaag accgctttcg ttgagcttta taatgttggc actgactctg aaactgtcga
     8941 gaccaagacc gtaagttcac caatattctc acttgagcca agcgtattat caggtgaaat
     9001 atgcaccgag atttccagcg aactgcttcc ggtaatacga gaagcaattc gccgtcgcgg
     9061 tttggatgga agttatgatc atgacgttct gcaagttctc aggacaatgg tgacttcact
     9121 ggggatttga tccctgcgtc ctctaagctt ttgatatagc ggtcctccaa gaagcggtaa
     9181 attttgtcga aatcatcttt tccctct
//
LOCUS       DAJVXP010000149         9188 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVXP010000149 DAJVXP010000000
VERSION     DAJVXP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.149"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="OQS10_003510"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="OQS10_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="OQS10_003511"
     CDS             complement(1751..2509)
                     /locus_tag="OQS10_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS2294452.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OQS10_003512"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OQS10_003512"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS2294453.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OQS10_003513"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OQS10_003513"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS2294454.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="OQS10_003514"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="OQS10_003514"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS2294455.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="OQS10_003515"
     CDS             complement(5474..6079)
                     /locus_tag="OQS10_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294456.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="OQS10_003516"
     CDS             complement(6082..6849)
                     /locus_tag="OQS10_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294457.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="OQS10_003517"
     CDS             complement(6852..7562)
                     /locus_tag="OQS10_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294458.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="OQS10_003518"
     CDS             complement(7578..9083)
                     /locus_tag="OQS10_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS2294459.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2261 g         2197 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactct ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAJVXP010000150         9042 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVXP010000150 DAJVXP010000000
VERSION     DAJVXP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.150"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..894)
                     /gene="glsB"
                     /locus_tag="OQS10_003519"
     CDS             complement(<1..894)
                     /gene="glsB"
                     /locus_tag="OQS10_003519"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS2294460.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQ"
     gene            complement(958..2346)
                     /gene="sad"
                     /locus_tag="OQS10_003520"
     CDS             complement(958..2346)
                     /gene="sad"
                     /locus_tag="OQS10_003520"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCS2294461.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2447..3328
                     /locus_tag="OQS10_003521"
     CDS             2447..3328
                     /locus_tag="OQS10_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2294462.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3405..4520
                     /gene="yneK"
                     /locus_tag="OQS10_003522"
     CDS             3405..4520
                     /gene="yneK"
                     /locus_tag="OQS10_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS2294463.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4670..5860
                     /gene="ydeA"
                     /locus_tag="OQS10_003523"
     CDS             4670..5860
                     /gene="ydeA"
                     /locus_tag="OQS10_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS2294464.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(5885..6550)
                     /gene="marC"
                     /locus_tag="OQS10_003524"
     CDS             complement(5885..6550)
                     /gene="marC"
                     /locus_tag="OQS10_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS2294465.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6762..7196
                     /gene="marR"
                     /locus_tag="OQS10_003525"
     CDS             6762..7196
                     /gene="marR"
                     /locus_tag="OQS10_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS2294466.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7216..7599
                     /gene="marA"
                     /locus_tag="OQS10_003526"
     CDS             7216..7599
                     /gene="marA"
                     /locus_tag="OQS10_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS2294467.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7631..7849
                     /gene="marB"
                     /locus_tag="OQS10_003527"
     CDS             7631..7849
                     /gene="marB"
                     /locus_tag="OQS10_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS2294468.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(7906..>9042)
                     /locus_tag="OQS10_003528"
     CDS             complement(7906..>9042)
                     /locus_tag="OQS10_003528"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS2294469.1"
                     /translation="PNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWR
                     NRKLIDFFIRFASTVFTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDI
                     NEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATK
                     AMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSF
                     TTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVE
                     NGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGT
                     GEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2318 a         2311 c         2056 g         2357 t
ORIGIN      
        1 ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt ccggactcca
       61 gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa cgccagattt
      121 cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac cactggtcgc
      181 catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat caatatgaat
      241 agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac agctcatttt
      301 cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat ggaaattgcc
      361 aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa attcggaacg
      421 cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca cgacttccag
      481 catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca ccagcgcacc
      541 agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca ctaaagaatt
      601 gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt cggagtaatg
      661 acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa aacgttcttg
      721 cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc ccaatcggga
      781 accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac caatgagcgg
      841 ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg ccactttcta
      901 ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata tgtcgcgtca
      961 gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga aatgggaaag
     1021 ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt cgctggcaca
     1081 ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc tggcctgtgt
     1141 ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag ccagttccag
     1201 cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca tttcttcccg
     1261 aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt agttacctgc
     1321 cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg ttttctctac
     1381 ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag tgttctcttc
     1441 gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt cggtaaatgc
     1501 agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct gtccggtatt
     1561 ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat cgttaagcac
     1621 aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg ctccagcctg
     1681 tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag caatgcgcga
     1741 gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg
     1801 cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca tcacattcgg
     1861 cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac
     1921 ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg gtcgatactc
     1981 aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg ccggaccatg
     2041 ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg cctggttgat
     2101 tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg cgcgcagagc
     2161 cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc gccagtcgcg
     2221 aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag cccacggcag
     2281 cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg ccggagtaat
     2341 ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt tactcttgct
     2401 ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg atctgaccca
     2461 actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg cagcaaaagt
     2521 gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag aactgggggt
     2581 agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc ataacttttt
     2641 acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg tcgctggcga
     2701 tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag tgcgcattcc
     2761 ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac tttccaccgg
     2821 cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg catttattga
     2881 tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg agctgatgat
     2941 cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg gcagtaacat
     3001 ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt ttcatgctga
     3061 cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt tggcctgtgt
     3121 ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta tgccggggca
     3181 tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa cctggctggt
     3241 ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac tggatgtgtc
     3301 tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg ctaatatgct
     3361 ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt acgccagtaa
     3421 gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt gcaccacagg
     3481 taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt gatgaagagt
     3541 ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac cttgataact
     3601 tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat gaaaaggttc
     3661 agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt gaatttcagg
     3721 tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga gaaattaata
     3781 ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag accggcgtcg
     3841 agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg ttcaaccctg
     3901 gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg gatcaccata
     3961 ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc tctcgcggta
     4021 attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg ttcgtcaata
     4081 aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta aaagaggatt
     4141 tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc gaagtctgca
     4201 tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac ttgtttttat
     4261 ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa agtgatttga
     4321 atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat atggcgcgca
     4381 ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac gaagacctgg
     4441 tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc aaaacgccag
     4501 acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga acagaatcag
     4561 cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac atcattaacc
     4621 gttttaagat catttcatca ctttttcgca actcacccga taatctgtta tgacaacaaa
     4681 cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg ccgccttcat
     4741 cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc aaagttttca
     4801 catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag tagcgctaat
     4861 gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac tgatctgcct
     4921 gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct ttaccgttct
     4981 ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga ttacggcgtc
     5041 tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt taattgccac
     5101 cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg ggcagtattt
     5161 cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc ttttgtgcct
     5221 gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa gcctcccgct
     5281 attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg ttgtcaccgc
     5341 ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg gattcagcgc
     5401 caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg gcagcgtgat
     5461 tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga ttgcgctgtt
     5521 gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc tcggggtgct
     5581 gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg ttaaagtgct
     5641 ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca tatttaatat
     5701 tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt caatgtcgat
     5761 gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa tcattatatt
     5821 tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc cattcgggcc
     5881 ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac tgcacaccca
     5941 tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc ttgcccacca
     6001 gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc accaggaaaa
     6061 agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc tgacgcactg
     6121 tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt ggcattgcta
     6181 gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac ttgcttttcg
     6241 cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg ataaacgcga
     6301 cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta tccatcacca
     6361 gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag gccatcaacg
     6421 actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat agcgcgacag
     6481 ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt gctttaaaca
     6541 aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa gtataagggg
     6601 taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc aaaacttgaa
     6661 ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact tgcctgggca
     6721 atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt accagcgatc
     6781 tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag aagaaagatc
     6841 gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt aaggtgctct
     6901 gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg ttgtcggtcg
     6961 acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg gttgaaaggt
     7021 tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc ggcgcggcaa
     7081 tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta acaaaaaacc
     7141 tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg ccgtaaacaa
     7201 aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca tagcattttg
     7261 gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc agagcgttcg
     7321 ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca ttcattaggc
     7381 caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga aagtaacgag
     7441 ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct gacccgaacc
     7501 ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat gcagggcgaa
     7561 tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg acaacgtcac
     7621 tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc tcttttccgc
     7681 gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca atgtcgtggt
     7741 tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta ctggcagtga
     7801 taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt tattgtcagg
     7861 ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa tattttcgcc
     7921 attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc gttccagcgt
     7981 tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt tcatttcccc
     8041 tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta agtcaacacc
     8101 atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat caatgcgata
     8161 gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc cattttcgac
     8221 aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac gtagccctgc
     8281 cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga ccaaatctcg
     8341 tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa aagacatgta
     8401 gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat cttctggtgt
     8461 gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg gataatatcc
     8521 acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg ccatcatcat
     8581 atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac agccaatgtt
     8641 aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa ctaactcgta
     8701 atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg gcgaatacaa
     8761 gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat tattgatttc
     8821 gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg ttgatgcgaa
     8881 gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg tcaccagatg
     8941 ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct tcaggcattc
     9001 atcgaacaga tcatcataaa attgtaaacc ctcttcattc gg
//
LOCUS       DAJVXP010000151         8720 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVXP010000151 DAJVXP010000000
VERSION     DAJVXP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.151"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1489
                     /locus_tag="OQS10_003529"
     CDS             <1..1489
                     /locus_tag="OQS10_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294470.1"
                     /translation="GLQQAGEQLLKTVLTRLRQHKLPASTADMAAAHLHAMALAQLRG
                     HKLPLRTDWLDAIAGSLIKEALNAPLPWSYRGVIHPDTDPILLTLIDTLAGDGFGKLA
                     PSTPQPPLPKDVTCELERTAISLPAELTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQ
                     GSTLTLAGNGEERWKLTRPLSQHAALIEAACFGATLQEAARHKLEADMLDAGGIGSIT
                     TCLSQAALAGLASFSQQLLEQLTLLIAQENQFAEMGQALEVLYALWRLDEISGMQGAQ
                     ILQTTLCAAIDRTLWLCESNGRPDEKEFHAHLHSWQALCHILRDLHSGVNLPGVFLSA
                     AVALLERRSQAIHVPALDRGAALGALMRLEHPNASAEAALTMLAQLSPAQSGEALHGL
                     LALARHQLACQPAFIAGFSSHLNQLSDDDFINALPDLRAAMAWLPPRERGTLAHQVLE
                     HYQLAQLPVSALQMPLHCPPQAIAHHQQLEQQALASLQNWGVFHV"
     gene            1482..2618
                     /locus_tag="OQS10_003530"
     CDS             1482..2618
                     /locus_tag="OQS10_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS2294471.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            2615..4615
                     /locus_tag="OQS10_003531"
     CDS             2615..4615
                     /locus_tag="OQS10_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCS2294472.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            4737..5198
                     /locus_tag="OQS10_003532"
     CDS             4737..5198
                     /locus_tag="OQS10_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS2294473.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5239..5709)
                     /locus_tag="OQS10_003533"
     CDS             complement(5239..5709)
                     /locus_tag="OQS10_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS2294474.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(5756..6475)
                     /gene="yehT"
                     /locus_tag="OQS10_003534"
     CDS             complement(5756..6475)
                     /gene="yehT"
                     /locus_tag="OQS10_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS2294475.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(6472..8157)
                     /gene="btsS"
                     /locus_tag="OQS10_003535"
     CDS             complement(6472..8157)
                     /gene="btsS"
                     /locus_tag="OQS10_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS2294476.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            8379..8444
                     /locus_tag="OQS10_003536"
                     /pseudo
     CDS             8379..8444
                     /locus_tag="OQS10_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2145 a         2296 c         2215 g         2064 t
ORIGIN      
        1 gggcttacag caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca
       61 caaattgcct gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc
      121 acagttgcgc ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc
      181 gctgattaaa gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc
      241 cgacaccgat ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa
      301 acttgcccct tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg
      361 taccgcaatc tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc
      421 gcaaagtcag gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca
      481 gggaagcaca ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct
      541 tagccagcat gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc
      601 acgccataaa ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg
      661 tcttagccag gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact
      721 cacattatta atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct
      781 atatgcctta tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac
      841 gacgttatgc gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga
      901 tgaaaaggag tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga
      961 tctacatagc ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga
     1021 gcgccgcagt caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact
     1081 gatgcgtctg gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt
     1141 atccccggca caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact
     1201 ggcctgccaa ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga
     1261 cgattttatt aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga
     1321 acgcgggaca ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc
     1381 agcgctgcaa atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga
     1441 acagcaggca ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc
     1501 ttctgaccac ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga
     1561 cgctttgtgg gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg
     1621 ggcgcgatcc tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc
     1681 tcaccactcc tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc
     1741 ggctggaaag cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca
     1801 tgctggaacg tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc
     1861 tgatgaatcc cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa
     1921 ttatggctcg actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc
     1981 gccgctcatt tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc
     2041 tgcaacactg gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc
     2101 gcattaaacg ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga
     2161 tggtggactc ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca
     2221 ttcgtaccca tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg
     2281 atccggtaga gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg
     2341 tggagtatgg tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg
     2401 atttttacga agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga
     2461 gcggcatcaa agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc
     2521 gcgatacggc ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg
     2581 agctggcatc atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt
     2641 agaactgaca ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct
     2701 taaggaactg gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc
     2761 caccttcagt gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca
     2821 gtgcagttgc ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca
     2881 acgactttgt gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg
     2941 gctggaagaa ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc
     3001 taaaggcatc accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc
     3061 gatgagcgat atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat
     3121 tgaaagcaca ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc
     3181 gcagcaagcg gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc
     3241 tgacgatccg tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag
     3301 ccaggcatta tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag
     3361 ccgcgcgcag caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca
     3421 actgcgcgcc agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg
     3481 cttacgtcag cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg
     3541 cgacagtatt ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc
     3601 cggagaagca aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga
     3661 tattgagcat tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca
     3721 cctttcgcgc agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct
     3781 gtttagtttt caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg
     3841 cagtgccgat ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct
     3901 gtcgcctgat gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt
     3961 gcgggaatat ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa
     4021 cttatttatt ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac
     4081 gctggatgcg caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc
     4141 ggcatctgcc tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga
     4201 cgaccccgtg agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga
     4261 accacgggtg atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt
     4321 ggcaccgcta ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat
     4381 gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag
     4441 cgctattagt caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct
     4501 ggcacatgtg ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa
     4561 taacggtgtt ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag
     4621 tgcttactaa gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc
     4681 tatatagccc ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga
     4741 aggctttcaa taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg
     4801 gctgcggtga caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg
     4861 ccattaccta tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc
     4921 aatttgcctc cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa
     4981 gcgccaaata caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg
     5041 cgcaggaaaa cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt
     5101 caggaatcaa cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac
     5161 tggtgatgaa agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc
     5221 atggccgcca tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag
     5281 aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat
     5341 catcgctgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat
     5401 gtcatcggtt ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat
     5461 gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag
     5521 gccattgagg aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc
     5581 tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat
     5641 acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt
     5701 acttagcatg aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca
     5761 ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat
     5821 tacgcaaaat caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga
     5881 ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta
     5941 atgtcaattc ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc
     6001 gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac
     6061 aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc
     6121 gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca
     6181 gataatcaaa ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa
     6241 aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc
     6301 gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc
     6361 cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata
     6421 cacgcaggtt ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc
     6481 gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct
     6541 tattccatag tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag
     6601 cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa
     6661 atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt
     6721 cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg
     6781 ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc
     6841 aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg
     6901 ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc
     6961 gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt
     7021 cacctgggcg tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata
     7081 ttgcccggca agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt
     7141 tattgaactg aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg
     7201 attttcgcca cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga
     7261 gcaacgataa ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt
     7321 taaggtgtaa ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt
     7381 aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata
     7441 cagcacctga gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc
     7501 cgtcgaggct gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat
     7561 cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat
     7621 cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc
     7681 caggatgatc agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg
     7741 gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag
     7801 caatccttca acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata
     7861 tcgatgtaag ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc
     7921 gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa
     7981 ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag
     8041 acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat
     8101 gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc
     8161 cgcaaaccac acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg
     8221 aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat
     8281 aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc
     8341 taaagttaaa ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg
     8401 aagtggcgtt gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta
     8461 aaaatcgacg aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt
     8521 accatgtcga cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt
     8581 gatgccccat aaacaatatc aggaattgac actggttatc catacagata aaaataacac
     8641 ctgtacataa ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga
     8701 aaaaatcact aaaatgccaa
//
LOCUS       DAJVXP010000152         8697 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVXP010000152 DAJVXP010000000
VERSION     DAJVXP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.152"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            812..1054
                     /locus_tag="OQS10_003537"
     CDS             812..1054
                     /locus_tag="OQS10_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294477.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OQS10_003538"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OQS10_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS2294478.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OQS10_003539"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OQS10_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS2294479.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OQS10_003540"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OQS10_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS2294480.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OQS10_003541"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OQS10_003541"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS2294481.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="OQS10_003542"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="OQS10_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS2294482.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8697
                     /locus_tag="OQS10_003543"
     CDS             8087..>8697
                     /locus_tag="OQS10_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS2294483.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHA"
BASE COUNT         2066 a         2208 c         2235 g         2188 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttctc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgc
//
LOCUS       DAJVXP010000153         8666 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVXP010000153 DAJVXP010000000
VERSION     DAJVXP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.153"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(79..915)
                     /gene="bglG"
                     /locus_tag="OQS10_003544"
     CDS             complement(79..915)
                     /gene="bglG"
                     /locus_tag="OQS10_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS2294484.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(1201..1926)
                     /gene="phoU"
                     /locus_tag="OQS10_003545"
     CDS             complement(1201..1926)
                     /gene="phoU"
                     /locus_tag="OQS10_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS2294485.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1941..2714)
                     /gene="pstB"
                     /locus_tag="OQS10_003546"
     CDS             complement(1941..2714)
                     /gene="pstB"
                     /locus_tag="OQS10_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS2294486.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2805..3695)
                     /gene="pstA"
                     /locus_tag="OQS10_003547"
     CDS             complement(2805..3695)
                     /gene="pstA"
                     /locus_tag="OQS10_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS2294487.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3695..4654)
                     /gene="pstC"
                     /locus_tag="OQS10_003548"
     CDS             complement(3695..4654)
                     /gene="pstC"
                     /locus_tag="OQS10_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS2294488.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4741..5781)
                     /gene="pstS"
                     /locus_tag="OQS10_003549"
     CDS             complement(4741..5781)
                     /gene="pstS"
                     /locus_tag="OQS10_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS2294489.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(6029..7102)
                     /locus_tag="OQS10_003550"
     CDS             complement(6029..7102)
                     /locus_tag="OQS10_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2294490.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(7113..>8666)
                     /locus_tag="OQS10_003551"
     CDS             complement(7113..>8666)
                     /locus_tag="OQS10_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2294491.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2242 a         2203 c         1952 g         2269 t
ORIGIN      
        1 gcctgacgtg aatcaagcaa tttttttcag gttttgcccg cttagtgcgg taacaatcct
       61 ttactcagta ataatatttc agtgttcttt gcgcacgcgc tctatattta tggctaaaaa
      121 cataatctct gcgggtgaaa ttttacgttg atactgcaaa ccaataaaaa tggcgatccg
      181 ttccgcacat tgccatgctt gcgggtaatt ttgttttact gcttgttgta atgattcatc
      241 actatcgtta attgaagcat gttcaagaat acgccaggat aaaaacttca gatgtgtaac
      301 cagtcgctga taactcaagc tttcttcctg gtaattaagg ctgaactgaa attttattaa
      361 ttgcagcatt tcgcgcatta actgcgtgac acctgcaaca tcctccatat ttccgctcat
      421 ttgggcactg accagatgca tggcaataaa gcccacttca tctttcggta actgcacgcc
      481 caaccgttta tcaataatgg ttaacgcttc ttcccctagc tggaactctt tcgggtaaag
      541 ccgctggata tcccacagca acgggttggg cagcaacacg ttttgctgaa agcgtttaat
      601 cgcaaactgg caatggtcag ttagcgagat ataaatactg tcctgtaatt ttcccaagcg
      661 ctcctgcgct aaagagataa tacgatcaca ggttgccatc acctcaagag gaatatgact
      721 taagagttcg cttaatcgcc cgttcagttc atgactgctc aaggcatact ctttttctat
      781 tccacttgag ttaattcttt cgccagcgca tttttgaaag ccaattcctc gccccatgac
      841 gactttttcc cgctgttgat catcaataac caccacaaca ttattgttga gaattttggt
      901 gatttgcatg ttcatagcaa ggaccttttt tataaacaaa aaaaaccgac ttcaccagta
      961 ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac aatccagtca tttattaatg
     1021 ttttttataa cgaacatcca ggttcggaaa ttaatttaat tgcgtgcaga gaaaatagca
     1081 atgcgctatt gataaaaata tgaccatgct cgcagttatt aactttgtgt aattttagga
     1141 atttataaag ttatatataa caaatcccaa taattaagtt attgggattt gtctggtgaa
     1201 ttatttgtcg ctatcttttc ccgccagcag tttatccagc tcatcaccgc ctacatgacg
     1261 gaaatcctgc cccttcacgt agtagaagat aaactcacaa atattctggc agcggtcgcc
     1321 aatacgttcg atagaacgcg cgcagaacag cgcagtaagt acgctcggaa tggtacgcga
     1381 atcttccatc atgtaggtca tcagttgacg aacaatacct tcgtattcct gatcgacttt
     1441 tttatcttca cgataaatac gtaccgcttc gtcaatgtcc atccgcgcga acgcgtccag
     1501 cacgtcgtgc agcatctgga tggtatgacg gcccagcgac tccagactta ccagcaacgg
     1561 ctgatgctgc tgggagaatt tctccagcgc agtacggcag attttgtccg ccacgtcgcc
     1621 aatacgctcc agctcggcaa tggttttact gatcaccata accagtcgca ggtcgctcgc
     1681 cgtcggctga cgtttggcga taatgcgcac gcaggcttca tcgatcgcca cttccatcat
     1741 gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga tcgctgtcct ggttatgcat
     1801 cgcggtgatt gcatcagaaa gctgctgctc caccatgcca cccatggtca tcacctgcgt
     1861 gcggatactt tccagttcgg cgttgaactg gccggaaata tgtttattaa gattgagact
     1921 gtccataacg cactcctgaa ttaaccgtaa cgaccggtga tgtagtcttc tgtttgtttc
     1981 ttcgctggct tggtgaacag atcgtccgtg ttgctgaact caatcaattc gcccaggtac
     2041 ataaacgccg tgtggtcgga acaacgcgca gcctgctgca tgttgtgggt aacgatcacc
     2101 acggtgtaat cctgcttcag ttcggtgatc agctcttcaa tacgcccggt agagatgggg
     2161 tcgagcgccg aacacggttc gtcgagcagc agcacttccg ggcgaatggc gataccacgc
     2221 gcaatacaca gacgctgttg ctgaccacca gagagagagt aaccgctctg gtgcaattta
     2281 tctttggttt cgttccacaa tgcggctttg gtcaatgccc actgcacgcg ctcgtccatg
     2341 tcggcacggg agagcttctc aaacagacga acaccaaaag cgatgttgtc gtagatggac
     2401 atcgggaacg gcgtcggttt ctggaacacc atgcccactt tcgcacgcag cagtgcgata
     2461 tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa tttcaccttc tgcgcgctgc
     2521 tccggataca gttcaaacat tttgttgaag gtacgcagca gcgtcgattt accgcagccg
     2581 gacggcccga taaacgccgt tacctggttt ttagcgatat ccaggttgat gtttttcagg
     2641 gcatggaatt tgccgtagta gaagttcaaa ttacgaacct gaattttact cggggcagtt
     2701 tcaaccatac tcattgcaat cttttcctca tcgtacctga tgcttttcgc taatcaggct
     2761 taccgtaggc cgggtaaggc gttcacgccg catccggcaa agtgttaacc gtgtttattc
     2821 ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagga cgcacagggt aatgatcaat
     2881 accccggccc aggccagttg ttgccattcc gcaaacgggc tcatcgcaaa cttaaagatc
     2941 gtcaccggca ggttggcgat cggctgcatc atgtccgtgc tccagaactg gttggagagc
     3001 gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg caatcgccag caggataccg
     3061 gtcataatcc cggacaccga cgctttcagc gtaatcgcgg agatcatctt ccacttcggt
     3121 gtacccagcg cataagccgc ttcacgcagg ctgtccggca ccagtttcag catgttctcg
     3181 gtggtgcgga taacgatcgg cacctgcaac agcgccagcg caattacgcc cgcccagccg
     3241 gagaagtgct ccatctgtgc caccacgatg gtgtagacaa acagaccaac cacaatcgac
     3301 ggcacggaga gcagaatgtc gttaatgaag cgaatcactt ctgccagcca ggatttacga
     3361 ccatattccg ccagataaat ccccgccata atgcccagcg gcgtaccgaa taccgtggcc
     3421 cacaaaatta acagcccgct acccgccagg gcgttcgcca gaccaccacc ttccgtattg
     3481 ggcggcggcg tcatttcagt gaacagcgcc agcgacatac cgtcgatacc gcgagtgatg
     3541 gtggacatta aaatccagat cagccagaac aggccgaagg ccatcgtcgc catcgagagc
     3601 gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca ttttgcggcg agattcagcc
     3661 agcgccgcag tggtttgcat ttcaaccata gccattagcg tgccccctca ttcttagcca
     3721 ggcgcataat cataaacttc gatgcggcga ggacgatgaa ggtaatcaca aacaggatca
     3781 ggcccagttc catcagtgcg gcaacgtgca gaccggattc cgcttccgca aattcgttcg
     3841 ccaacgcaga ggtgatacta ttgcccggca tgtacagcga ggcgctgtcg agctggtagg
     3901 tgttaccgat gataaaggtc accgccatgg tttcaccgag cgcgcggccc agccccagca
     3961 tgatgccgcc gataacacca tttttggtga acggaagaac gatacgccag ataacttccc
     4021 aggtggtgca gccaataccg taggccgact ctttcatcat caccggggtt tgttcgaaca
     4081 catcacgcat taccgccgca atgtacggaa taatcatgat ggcgaggatc acgcctgccg
     4141 cgaggatacc gataccaaat gcggggccag agaacagcgc gccaacaatc gggatattcg
     4201 acatgatatt gccgaccggc tcctgaaagt aaacggcgaa cagcggcgca aagataaaca
     4261 ggccccacat gccgtaaacg atacttggaa tggctgccag cagctcaatg gcgataccca
     4321 gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa cagggcgata ccgaaactca
     4381 ccgggacggc gatcagcagc gcgataaacg aagtcaccaa cgtaccgtag atcggcacca
     4441 gcgccccgta gatatcgttc ggtgcatccc actctttggt ccataggaaa gccagaccaa
     4501 atttctgaat gctcggccag gaggagatga tcagagagac aataatgcca cccaacatca
     4561 atagcacaat cagcgccgcc agttttacca gcacgctgaa aattatgtcg ccctttttac
     4621 ccggtgggtt aaaagcaggc ttggttgcag ccataagtta ctcttcagtt aaacgcgttt
     4681 acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc gtacccggcc tggagtttta
     4741 ttagtacagc ggcttaccgc tactgtcttt aatattggtc ttccacgcag cgcgaacctg
     4801 ttcaactaca ctatccggca ggctggcgta atccaggtcg ttcgcctgtt tagccccggt
     4861 tttgtacgcc cagtcgaaga atttcagcac ttctgtgcct tgttctggtt tcttctgatc
     4921 tttgtggatc agaatgaacg tggtagaggt aataggccat gcatcttcgc ctttctggtt
     4981 ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct tttgctgcat tagcgaagtt
     5041 ttcttcggtc ggactaaccg gtttaccatc agcggagatc agtttggtgt acgccaggtt
     5101 gttctgcttc gcgtaagcat attcaacata accaattgca cccggcagac gctgaacgaa
     5161 cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc ggccatttta cggtagagcc
     5221 agtaccaacg ttgtttttcc actcttcgtt cactttcgcc aggtagctgg tgaagacgaa
     5281 ggtagtcccg gagccatctg cgcggcgtac tacagcaatg ttttgtgaag gcagtttcag
     5341 acccggattc agtttggcga tggtttcatc atcccacttc ttgattttgc ccaggtagat
     5401 gtcgccgagg gttttaccat ccagcaccag ttcgccagac ttcagccctg gaatgttaac
     5461 cgccagcacc acgccgccaa tcacggtcgg gaactggaac agaccttcct gagccagttt
     5521 ttcgtcagac agcggcgagt cagaggcacc aaaatcaacg gtattagcga taatctgttt
     5581 tacgccaccg gaagaaccga taccctggta gttaacttta ttaccgattt ctttctggta
     5641 agtgtcagcc catttggcat acaccggcgc agggaaggtt gcacctgcac ctgtcaggct
     5701 tgcttctgca aacacagaga aagcgctcat cgataaggtc gcggcgacaa cagttgcgac
     5761 ggtggtacgc ataactttca taatgtctcc tgggaggatt cataaagcat tgtttgttgg
     5821 ctacgagaag caaaatagga caaacaggtg acagttatat gtaaggaata tgacattttt
     5881 atgacagaaa ggaattaaca aataacttac tgattttaaa tgtgaagatg aaaatcccac
     5941 atgtcatctt tttgtcatga tgcatttaaa agtgataaag aaatgggtga gatgacaacg
     6001 atgtgatgaa caatcgttgt cagacctatt agcgcacaat ctcaacagtt atcgtcgctg
     6061 ttgcagtaaa ttgccccggg gctgggcgag ctccagtaag gctcgcagga gcagctgaaa
     6121 aagttacact acctgatttt tcaccataca ctgttgattt acccatgtct gttggcaact
     6181 caccaccgtt cactttgatt tccttattat ttttgtcgta aatttttatc cccacgttag
     6241 agtttgaagt tacgaccatt gaattgtccg tattgggttc ggtagcataa aaagacgtac
     6301 tgatatcatc ggtattcagt atccccaggt tttcacactc aatagtgact gttttagtaa
     6361 ttttatgtgt tgttaccgca cttccaacaa cagatgaaaa atctgcagct ggtatttctt
     6421 taaaatcaaa ataaactggc tgtaaattat ttatttcgca actttgcgga gctgtaatat
     6481 cgccccgaat ataaacatca gccatcggct gtgagtaatc caccggggtg tcacggctaa
     6541 ttgttccata caatgtcact atctttgttt caggaataat caattttcct gcaaatgtct
     6601 tcttgatata aaaatatact atcgctttac ccccactgga tatacttaaa ggttcaatgc
     6661 ggttgcaatt gtactttgta ttaccagacg ggttgttagc atgatattca aaaggtgcat
     6721 tcacataccc aaccccaaga atatctatag caatcccaac atcaaggttc ggattaagat
     6781 cataataatt aatattagag tgaaccccag cataagttaa tgcctggttt atttttgacg
     6841 tataatagat ataggggaat tctcctacat taccagtatc acaatgacag cgtccaggat
     6901 atgaggatcc attagttaca gtggtattaa atgtcgttcc aaccttattt tcgttcgcgg
     6961 taataagata tcccgagaaa tcaagcgtac ggtgaaatac gccattatca ggggtacaga
     7021 tacctttagg agctgcaagt gctacactac ttaacgagag cataaacaca gcaaagcacc
     7081 actgtttgat atacttgttc ataaaaatcc ttttaatgac atgtcacacc ctgttcaatt
     7141 aatccgctct cactttctgg tgtcgaaagt gaataattga agatgcattt tgttgaggcg
     7201 ttttctcccc aggataacgt aacctgtcct ttttctggta gaccagaaag ataaagttcg
     7261 ccacggtcac cgacaattcc tgaatctgcc aaacttgcat cttgtatcgc agcaatggct
     7321 ccaaaaggta ccggtttacc atttgcattt aacactctga agagaacgcg ataacccaca
     7381 tgagtcacaa actccgcttt aaccactgca ccacgcgtgg gaaccatcgt aacgacggta
     7441 ttatcaagtt ctatattcga cttcgagaaa tagttatcgc tgattgctac ccgattttca
     7501 tcataagggt ttagctgtgt cttgaccgta tagccgcgcc agtccgtcgc agcaccgcgc
     7561 atattatcta tttctaaccc ggcggcccca ggtgctttaa caagagcaat agtttcacct
     7621 aactcctgac ttaacgttac cccttcgctg tgtgccagaa ttccccctct gataccataa
     7681 ttcagcctct gggaattttg gctgtagtta tagctgccgt tcaccgtgcc ataagagcca
     7741 tcataggtca gggacatatt accgccaacc tcatcatcat gactgttata ggactgcatt
     7801 aaattccatg aaaggttatt tctttccagc gccgtaccgc tgagtccgag ttgatgtgag
     7861 ctaccggaat tatcgttttg cgaaacagaa taggtggcgt aactattacc ccaccagccc
     7921 aataaaggaa cagataaaga gagcatgtag atctgctcac tggtgttatc actggcatgg
     7981 ttgttccccg tcccctgtct ggcagtattg gaataactcc aggagacgtt ccacgagaga
     8041 cgcttccagc tactgctgta gccaaattgc attaatcgtt cgacatcatc ggtatgccag
     8101 tattgttgcc ggcttaatgt caggtataaa ttgccataat ctctccccaa cgactgcgtt
     8161 agcgtcccct ccacgcaact acgtcggtta cctgtttccc aaaagtcttc agggctgttg
     8221 cgccgcgatg cccactcatt gagggtatag tagccctcgg tggagtaacg atagccaacc
     8281 aagcgcaagc tagtgtctgt gtcgtcaaat cgttttgaat agagaaagcg atatgactga
     8341 cccgtttctg tatcatcatg gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt
     8401 gcgcccaaag cgccaagatc tttaccgaca cccagagctg cagaaccata attttcagca
     8461 gcctgcatac ctccatataa agtgatcccg tgggaaaaac catgaagtat ttgcgcctga
     8521 aggacgtccg gtgtaaatcc gtcttcatcg cgcaattcac ccacgctgac atcaacatct
     8581 gtctgacctt cacgtttaag aatcgctaat gaagcataag gttgggtgaa tcgttgttcg
     8641 ctaccgtctt cttctttgat ggttac
//
LOCUS       DAJVXP010000154         8635 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVXP010000154 DAJVXP010000000
VERSION     DAJVXP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.154"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="OQS10_003552"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="OQS10_003552"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS2294492.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="OQS10_003553"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="OQS10_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS2294493.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OQS10_003554"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OQS10_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS2294494.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OQS10_003555"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OQS10_003555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS2294495.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OQS10_003556"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OQS10_003556"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS2294496.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OQS10_003557"
     CDS             complement(7826..8137)
                     /locus_tag="OQS10_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS2294497.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OQS10_003558"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OQS10_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS2294498.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaat
//
LOCUS       DAJVXP010000155         8582 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVXP010000155 DAJVXP010000000
VERSION     DAJVXP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.155"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(342..4244)
                     /gene="hrpA"
                     /locus_tag="OQS10_003559"
     CDS             complement(342..4244)
                     /gene="hrpA"
                     /locus_tag="OQS10_003559"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS2294499.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4445..5050
                     /gene="azoR"
                     /locus_tag="OQS10_003560"
     CDS             4445..5050
                     /gene="azoR"
                     /locus_tag="OQS10_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS2294500.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5101..6417)
                     /locus_tag="OQS10_003561"
     CDS             complement(5101..6417)
                     /locus_tag="OQS10_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCS2294501.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6407..8164)
                     /locus_tag="OQS10_003562"
     CDS             complement(6407..8164)
                     /locus_tag="OQS10_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS2294502.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8180..>8582)
                     /locus_tag="OQS10_003563"
     CDS             complement(8180..>8582)
                     /locus_tag="OQS10_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HCS2294503.1"
                     /translation="GALLVLFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGL
                     VGGVITTMIASMIIGPLLTPLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGK
                     LLPGHGGILDRIDSLIFTAPVFFYFIRYCCY"
BASE COUNT         2129 a         2359 c         2028 g         2066 t
ORIGIN      
        1 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
       61 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      121 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      181 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      241 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      301 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      361 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
      421 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
      481 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
      541 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
      601 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
      661 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
      721 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
      781 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
      841 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
      901 caggagatac agtcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
      961 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1021 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1081 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1141 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1201 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1261 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1321 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1381 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     1441 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     1501 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     1561 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     1621 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     1681 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     1741 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     1801 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     1861 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     1921 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     1981 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2041 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2101 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2161 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2221 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2281 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2341 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     2401 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     2461 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     2521 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     2581 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     2641 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     2701 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     2761 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     2821 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     2881 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     2941 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3001 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3061 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3121 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3181 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3241 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3301 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3361 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     3421 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     3481 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     3541 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     3601 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     3661 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     3721 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     3781 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     3841 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     3901 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     3961 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4021 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4081 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4141 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4201 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4261 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4321 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4381 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     4441 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     4501 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     4561 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     4621 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     4681 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     4741 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     4801 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     4861 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     4921 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     4981 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5041 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5101 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5161 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5221 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5281 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5341 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     5401 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     5461 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     5521 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     5581 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     5641 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     5701 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     5761 tattttgatt cgacgttgat caggtttctg ataattccaa cgacggtcga cgggcaccat
     5821 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     5881 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     5941 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6001 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6061 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6121 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6181 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6241 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6301 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6361 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     6421 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     6481 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     6541 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     6601 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     6661 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     6721 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     6781 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     6841 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     6901 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     6961 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7021 gccagccaac actgttcagg taaattttgt cgattaagcg cccgaatgcg ttactgcctt
     7081 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7141 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7201 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7261 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7321 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7381 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     7441 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     7501 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     7561 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     7621 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     7681 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     7741 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     7801 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     7861 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     7921 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     7981 cagtatctgg catcgccaat tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8041 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8101 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8161 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8221 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8281 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8341 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     8401 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     8461 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     8521 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     8581 cg
//
LOCUS       DAJVXP010000156         8424 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVXP010000156 DAJVXP010000000
VERSION     DAJVXP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.156"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..81)
                     /locus_tag="OQS10_003564"
     CDS             complement(<1..81)
                     /locus_tag="OQS10_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS2294504.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGH"
     gene            373..894
                     /gene="fecI"
                     /locus_tag="OQS10_003565"
     CDS             373..894
                     /gene="fecI"
                     /locus_tag="OQS10_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS2294505.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            891..1844
                     /gene="fecR"
                     /locus_tag="OQS10_003566"
     CDS             891..1844
                     /gene="fecR"
                     /locus_tag="OQS10_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS2294506.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1931..4255
                     /gene="fecA"
                     /locus_tag="OQS10_003567"
     CDS             1931..4255
                     /gene="fecA"
                     /locus_tag="OQS10_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS2294507.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4300..5202
                     /gene="fecB"
                     /locus_tag="OQS10_003568"
     CDS             4300..5202
                     /gene="fecB"
                     /locus_tag="OQS10_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS2294508.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5199..6197
                     /gene="fecC"
                     /locus_tag="OQS10_003569"
     CDS             5199..6197
                     /gene="fecC"
                     /locus_tag="OQS10_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS2294509.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6194..7150
                     /gene="fecD"
                     /locus_tag="OQS10_003570"
     CDS             6194..7150
                     /gene="fecD"
                     /locus_tag="OQS10_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS2294510.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7151..7918
                     /gene="fecE"
                     /locus_tag="OQS10_003571"
     CDS             7151..7918
                     /gene="fecE"
                     /locus_tag="OQS10_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS2294511.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1843 a         2411 c         2342 g         1828 t
ORIGIN      
        1 atgtccggca gtgcttttgc cgttacgcac cacgccttca gtagcggagc aggaaggaca
       61 tctgatggaa atggaagcca cgcaagcacc ttaaaatcac catcatacac taaatcagta
      121 agttgacagc attaccgaaa tccctatcct ttagggcagt gaggatgtca ataatagcag
      181 accacatcat gagtcattct gaccgacaca tgccagaagc agaggctcca tcccatttta
      241 tacctacctt ataacactta gaaaaacaac atgttaaaaa tgtctattgg aaacaatttt
      301 atttccaatt gtaatgataa ctattctcat attaatatgg ctacgtgata attaactttt
      361 gatgcactcc gcatgtctga ccgcgccact accacagctt ccttaacatt cgagtcgctt
      421 tatggcacac atcacggctg gttgaaaagc tggctgacgc gcaaactcca gtctgctttt
      481 gatgcagatg acattgccca ggacactttt ttgcgggtaa tggtcagcga aacgctctcg
      541 acgatccgcg atcctcgctc cttcctctgc actatcgcca aacgcgtgat ggtggacctg
      601 tttcgccgaa acgcgctgga aaaagcgtat ctggagatgc tggcgcttat gccggagggg
      661 ggagcgcctt cacctgagga acgcgaaagc caactcgaga ccctacaact cctcgacagc
      721 atgctggacg ggctaaacgg caaaacacgt gaagcgtttc tgctttcgca actggatggt
      781 ctgacataca gcgagattgc gcacaaactc ggtgtttcca tcagctccgt gaaaaaatac
      841 gtggcgaaag ccgtcgagca ctgcctgctg ttccgtctgg agtatgggtt atgaatcctt
      901 tgttaaccga ttcccgccgt caggcgctgc gttcagcttc ccactggtat gccgtgctaa
      961 gcggcgagcg cgtcagccca caacaggaag cgcgctggca acagtggtat gaacaggatc
     1021 aggataacca gtgggcctgg cagcaggttg aaaacctgcg caaccagctt ggcggtgtgc
     1081 ctggcgacgt tgccagccgg gcgttgcacg atacccgcct cacccgccgt cacgtgatga
     1141 aaggattact gctgttgctc ggcgctggcg gaggctggca gctctggcag tcggaaaccg
     1201 gcgaaggtct gcgggcagat taccgcaccg ccaaaggcac agtcagccgt cagcaactgg
     1261 aagatggctc cctgctcacg ctgaataccc aaagcgcggc ggatatgcgt tttgatgcgc
     1321 atcagcgcac cgtccggctc tggtacggtg aaatcgccat tactaccgcg aaagatgccc
     1381 tgcaacgccc cttccgtgtc ctgacccgtc agggccagct cactgcttta gggacagaat
     1441 ttaccgtccg ccagcaggat aatttcacgc agcttgacgt gcagcagcac gccgtggaag
     1501 tgcttctcgc cagtgccccc gcgcaaaaac gcatcgtgaa cgctggtgaa agcctgcagt
     1561 tcagcgcctc tgagtttggc gcagtgaaac cgctggatga cgagagtaca agctggacga
     1621 aggacatcct gagcttcagc gataaaccgc tgggtgaggt gatagccacg ctaacccgtt
     1681 accgcaacgg cgtgctgcgc tgcgatcccg ccgttgccgg gctgcgcctg agcgggacgt
     1741 tcccgctgaa aaataccgat gcgatcctga acgttatcgc gcaaacgctt cccgttaaaa
     1801 ttcagtctat tacgcggtac tggataaaca tttcaccact gtaaggaaaa taattcttat
     1861 ttcgattgtc ctttttaccc ttctcgttcg actcatagct gaacacaaca aaaatgatga
     1921 tggggaaggt atgacgccgt tacgcgtttt tcgtaaaaca acacctttgg ttaacgccat
     1981 tcgcctgagc ctgctgccgc tggccggtct ctcgttttcc gcttttgctg cacaggttaa
     2041 tatcgcaccg ggatcgctcg ataaagcgct caatcagtat gccgcacaca gcggatttac
     2101 cctctcggtt gacgccagcc tgacgcgcgg caagcagagc aacggcctgc acggcgatta
     2161 cgacgtcgag agcggcctgc aacaactgct ggacggcagc ggactgcagg taaaaccgct
     2221 gggaaataac agctggacgc tggagcccgc gcccgcacca aaagaagatg ccctgaccgt
     2281 ggtcggcgac tggctgggtg atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg
     2341 tgacgtgatc cgccgtgagg atttcgccaa aaccggcgca accaccatgc gtgaggtact
     2401 taaccgcatc cctggcgtca gcgcgccgga aaacaacggc accggcagcc acgacctggc
     2461 gatgaacttt ggcatccggg gcctgaaccc gcgcctcgcc agccgctcga ccgtcctgat
     2521 ggacggcatc cccgtcccct tcgcccctta cggtcagccg cagctttcac tggctcccgt
     2581 ttcgctcggc aacatgaatg ccattgacgt ggtacgcggt ggtggtgcgg tgcgttacgg
     2641 accgcagagc gtgggcggcg tggtgaactt tgttacccgt gccattccgc aggactttgg
     2701 tatcgaggcg ggcgtggaag gtcagctcag cccaacctct tcacaaaaca acccgaaaga
     2761 gacgcacaac ctgatggtgg gcggcacagc ggacaacggt tttggcaccg cgctgctcta
     2821 ctccggcacg cgcggcagtg actggcgcga gcacagcgcc acccgcatcg acgacctgat
     2881 gctgaaaagc aaatatgcgc cggatgaggt gcacaccttc aacagcctgc tgcaatatta
     2941 cgacggtgaa gccgacatgc ccggtggcct gtctcgcgcg gattacgacg ccgatcgctg
     3001 gcaatccacc cgcccgtatg accgcttctg gggtcgtcgc aagctggcga gcctgggcta
     3061 ccagttccag ccagacagcc agcataaatt caacattcag gggttctaca cccaaaccct
     3121 gcgcagcggc tacctggagc aaggcaaacg catcaccctc tcgccgcgta actactgggt
     3181 gcgcggtatt gagccacgct acagccagat ctttatgatc ggcccttccg cgcacgaagt
     3241 gggcgtgggc tatcgctatg tgaatgaatc aacgcatgaa atgcgttact acaccgccac
     3301 cagcagcggg cagttgccgt ccggctcaag cccttacgac cgcgatacgc gttccggcac
     3361 cgaggcgcac gcctggtatc tggatgacaa aatcgacatc ggcaactgga ccatcacgcc
     3421 gggtatgcgt ttcgaacata tcgagtcata ccagaacaac gccatcacag gcacgcacga
     3481 agaagtgagc tataacgcac cgcttccggc gttgaacgtg ctctatcacc tgactgacag
     3541 ctggaatctt tatgcaaaca ctgaaggctc gttcggcacc gtacagtaca gccagattgg
     3601 caaggctgtg caaagcggca atgtggaacc ggaaaaagcg cgaacctggg aactcggtac
     3661 ccgctacgac gacggcgcgc tgacggcgga aatggggctg ttcctgatta actttaacaa
     3721 tcagtacgac tccaaccaga ccaacgacac cgtcactgca cgtggcaaaa cgcgccatac
     3781 cgggctggaa acgcaggcac gttacgatct gggtacgcta acgccaacgc ttgataacgt
     3841 ttccatctac gccagctatg cgtatgtgaa cgcggaaatc cgcgagaaag gcgacaccta
     3901 cggcaatctg gtaccattct ccccgaaaca taaaggcacg ctgggcgtgg actacaagcc
     3961 aggaaactgg atgttcaatc tgaacagcga tttccagtcc agccagtttg cggataacgc
     4021 caatacggtg aaagagagcg ccgacggcag taccggccgc attcccggct tcatgctctg
     4081 gggcgcacgc gtggtgtatg actttggccc gcagatggca gatctgaacc tggcattcgg
     4141 tgtgaaaaac atcttcgacc aggactactt catccgctct tatgacgaca acaacaaagg
     4201 catctatgca ggccagccgc gcacgctgta tatgcagggg gcgttgaagt tctgatgtgt
     4261 taacgcccgg cttgccgggc ttttagctgg aatgtgatta tgttggcatt tatccgtttt
     4321 ctttttgcag gcctgctgct ggtgatcagc cacgcctttg ccgccacggt tcaggacgaa
     4381 cacggcacgt ttacactcga aaaaacgcca caacggattg tggtgctgga actctcgttc
     4441 gccgatgcgc tggccgccgt ggacgtcagc ccgatcggta ttgccgacga taacgatgca
     4501 aaacgcatcc tgcccgaagt gcgtgcgcac ctgaaaccgt ggcagtccgt cggaacgcgc
     4561 gcgcagccga gcctggaagc cattgccgct ctgaaaccag acctgatcat tgccgacagc
     4621 agtcgccatg cgggggttta catcgccttg cagcaaatcg cgtcggtact gctgcttaag
     4681 tcccgcaacg aaacctacgc tgaaaatttg caatctgcgg ctatcatcgg cgaaatggtg
     4741 ggtaaaaagc gagagatgca ggcacgtctg gaacaacata aagagaggat ggcgcagtgg
     4801 gccagccagc ttcccaaagg gacacgcgtg gcctttggca catcacgcga acagcaattc
     4861 aacctgcata ctcaggagac ctggaccggc agcgtgctgg cctctctggg gctgaacgtt
     4921 cccgctgcga tggcgggtgc gtccatgccg tccatcggcc tggagcaact gctggcggtc
     4981 aatcctgcct ggctgctggt tgcccactat cgcgaagaga gcattgttaa acgctggcaa
     5041 caagatccgc tctggcagat gttaaccgcc gcgcagaagc agcaggttgc ttcggtcgac
     5101 agtaacacct gggcgcggat gcgcggtatt tttgctgcag agcgtattgc cgctgacacg
     5161 gtaaaaatct tccaccatca gccgcttacc gttgtgaaat gaccgcgata aaacacccgg
     5221 tgctgctgtg ggggcttccc gttgcagcac ttattattat tttctggctg agtctgtttt
     5281 gctactcggc cattcctgtt tccggagcag atgcaacccg cgccctgctg cctggacaca
     5341 cgccaacgct accagaagcg ctggtgcaaa accttcgttt gccacgaagc ctggtcgccg
     5401 ttctgatcgg cgcaagcctg gcgctcgcgg gcacgctgct gcaaaccctg acccacaacc
     5461 caatggcctc tccttcactg ctcggcatta acagcggcgc ggcgctggct atggcgctta
     5521 ccagcgcgct gagtccgacg ccgattgcag gctattctct gtcgttcatc gcggcatgcg
     5581 ggggcggcgt gagctggctg ctggtcatga ccgcaggagg cggatttcgt catacccatg
     5641 acagaaacaa actgatcctc gcgggtatcg cgctgtcggc cttttgtatg ggccttaccc
     5701 gcatcaccct gctgctggcc gaagatcatg cttacggcat cttttactgg ctggctggcg
     5761 gagtgtccca cgcccgctgg caggatgtct ggcagctctt gccggtggtg gtcactgcag
     5821 tccctgtcgt gttgctgctg gcgaatcaac tgaacctgct caacctcagc gacagcaccg
     5881 cccatacgct gggagtgaac ctgacgaggc tacgtttggt catcaatatg ttagtgctgc
     5941 ttctggttgg tgcgtgcgtc agcgtggcag gtccggtggc gtttatcggt ctgctggtgc
     6001 cacatctggc gcgcttctgg gcaggcttcg atcagcgcaa cgtactgcca gtgagcatgc
     6061 tgctgggggc cacgctgatg ctgctggcag atgtactcgc acgcgcgctg gccttccccg
     6121 gagatctgcc cgcaggcgca gtgctggcgc tgattggcag cccttgcttt gtctggcttg
     6181 tgaggaggcg aggatgaaaa ttgcgctggt tattttcatc acccttgccc tggcgggctg
     6241 tgcgctgtta tcactccata tgggagtgat ccccgtgccg tggcgcgcgc tgctgaccga
     6301 ctggcaggcc ggacgcgagc attattatgt attgatggag taccgactgc cgcgcttgct
     6361 gctggcactg tttgtcggtg cagccctcgc cgtggcgggc gtgctgatac aggggattgt
     6421 gcgcaaccct ctggcatcac cggatattct cggcgttaac catgccgcca gcctggcctc
     6481 tgtgggggct ctacttctta tgccgtcact gcccgtgatg gtgctgccgc tgctggcctt
     6541 tgcgggcggc atggcggggt tgatattgct gaagatgctg gcaaagaccc accagccgat
     6601 gaagctggca ctcaccggcg tggcgctttc tgcatgctgg gccagcctga cggattatct
     6661 gatgctctcg cgcccgcagg atgtgaacaa cgccctgctg tggctgaccg gcagcttatg
     6721 gggccgtgac tggagctttg tgaagattgc catcccgctg atgattttat ttctgccgct
     6781 gagcctgagt ttttgccgcg atctcgacct ccttgcactc ggcgatgcgc gcgccaccac
     6841 gctcggtgtg tcggtgccct atacccgatt ctgggctttg ttactagctg tcgccatgac
     6901 atctaccggc gtggccgcct gcggcccgat tagctttatt ggtctcgtgg tgccgcatat
     6961 gatgcgtagc atcaccggtg gacgtcaccg cagactgctg cctgtttcgg ccctgacagg
     7021 tgcgttgctg ttggtggttg ccgatctgct ggcgagaatt attcatcccc cactggagct
     7081 cccggttggc gtgctgaccg ccattatcgg tgcgccgtgg tttgtctggt tgcttgtgag
     7141 aatgcgataa atgactttac gaactgaaaa tctgacggtc aattacggaa cagacaaggt
     7201 acttaacgac gtttcactct cactgccaac ggggaagatc accgccctga tcggtcctaa
     7261 cggttgcggg aaatcgacgc tgttaaactg tttttcgcgg cttttaatgc cgcagtctgg
     7321 caccgtattt ctcggcgata atcccataaa tatgctctca tcgcgccagt tggcccgcag
     7381 gctttcgctg ctgcctcagc accatttaac gccagagggg atcacagtcc aggagctggt
     7441 ttcgtatggt cgtaatccct ggctgtcact ctgggggcgt ctctccgctg aagacaatgc
     7501 acgagttaat gtcgccatga accagacccg gatcaatcat cttgccgttc gtcggttaac
     7561 cgagctttcc ggcggtcagc gccagcgcgc atttctggca atggtcctgg cccagaatac
     7621 gcccgttgta ttacttgatg agccaaccac ctatcttgat atcaatcacc aggtggacct
     7681 gatgcggttg atgggcgaac tccggactca ggggaaaacg gtggtcgctg tgctgcacga
     7741 ccttaatcag gctagccggt actgcgatca actggtggta atggcaaacg gacatgttat
     7801 ggcgcaaggc acaccagaag aggtgatgac cccaggattg ctgagaacag tattcagcgt
     7861 ggaagcggaa atacaccccg agccggtatc tggcaggccg atgtgcctaa tgaggtagat
     7921 tgcacaggcc gtaagaacca aaccacgact gaatgaaact ggactggcgc cagcaagcct
     7981 gttcagactg gggctgaact tttccggact ctgaaagatt accaatactc atcgtccatc
     8041 cgcttgcttt aggctgacat gttcataatc aacgcaaacc agagctgtac aggcttgggc
     8101 gcggctttca aaccagtcgt gatcacggca atcaattttg aactctgctt aacggacatt
     8161 tctgtataac ccttacggca acgaaaaacg cgaagttaaa attttagaaa cccaaaaacg
     8221 tgacatgact aagtttagat ttcagggggg ggagatcaaa aaatttcgct ctgtgccaga
     8281 gcggacattc acggagctgg ttcattacca atgaggttgg gcttttgagg ataaatcaat
     8341 gatcagacgc caacgtaaat caaaagcaac cctggaacgg aattgctaat ccagtttctg
     8401 accatcgatt tttctaaaaa gtgt
//
LOCUS       DAJVXP010000157         8332 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVXP010000157 DAJVXP010000000
VERSION     DAJVXP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.157"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(340..1176)
                     /locus_tag="OQS10_003572"
     CDS             complement(340..1176)
                     /locus_tag="OQS10_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS2294512.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1176..1979)
                     /locus_tag="OQS10_003573"
                     /note="aph(3'')-Ib"
     CDS             complement(1176..1979)
                     /locus_tag="OQS10_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS2294513.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2040..2855)
                     /gene="sul2"
                     /locus_tag="OQS10_003574"
     CDS             complement(2040..2855)
                     /gene="sul2"
                     /locus_tag="OQS10_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS2294514.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4044..4193
                     /locus_tag="OQS10_003575"
     CDS             4044..4193
                     /locus_tag="OQS10_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294515.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4949..5389)
                     /locus_tag="OQS10_003576"
     CDS             complement(4949..5389)
                     /locus_tag="OQS10_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS2294516.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5607..5996
                     /locus_tag="OQS10_003577"
     CDS             5607..5996
                     /locus_tag="OQS10_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294517.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6040..6228
                     /locus_tag="OQS10_003578"
                     /pseudo
     CDS             6040..6228
                     /locus_tag="OQS10_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6195..7394)
                     /gene="tet(A)"
                     /locus_tag="OQS10_003579"
     CDS             complement(6195..7394)
                     /gene="tet(A)"
                     /locus_tag="OQS10_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS2294518.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7500..8150
                     /gene="tetR(A)"
                     /locus_tag="OQS10_003580"
     CDS             7500..8150
                     /gene="tetR(A)"
                     /locus_tag="OQS10_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS2294519.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT         1885 a         2296 c         2306 g         1845 t
ORIGIN      
        1 gctcgcgggc gaggtccagg gcggccgtct tcacgttctg ccttgcgcag atgagataga
       61 tctagcgtgg actcaaggct ctcgcgaatg gctcgcgttg gaaactttca ttgacacttg
      121 aggggcaccg cagggaaatt ctcgtccttg cgagaaccgg ctatgtcgtg ctgcgcatcg
      181 agcctgcgcc cttggcttgt ctcgcccctc tccgcgtcgc tacggggctt ccaggggtcg
      241 tttgcgggaa ggggcggaat cctacgctaa ggctttggcc agcgatattc tccggtgaga
      301 ttgatgtgtt cccaggggat aggagaagtc gcttgatatc tagtatgacg tctgtcgcac
      361 ctgcttgatc gcggccgcga tagctagatc gcgttgctcc tcttctccat ccgcgttcca
      421 agctgcggaa aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg gatcgacgtc
      481 cagcgcacga gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga gacaaaggtc
      541 gtctctgtca gccggatcgt agaacatatt ggcggcgcca aagcccactt caccgaccag
      601 accgacggga tctatcacca gccagccgcg actggagaac atgatgtttt catgatgcag
      661 atcgccatgt agcccacgca gttccgaggc attgctcatc atttgatcgg ctataatcgc
      721 cgcgtggacg tagtcagttt gacaacctgc gttttgatca tcgcgcgccc gctgaaacaa
      781 agctgcaaag cgatcccgga tcgggagaag ggcagaaggc aggggttcct cagatgcggc
      841 atacagcttc gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc cgtgctcggc
      901 aacgatgtga gagagcattc gctccccggc atattcgagc aacatcagat tgttctcacg
      961 accgagcaac cggactgctc ccctcccatt gcgccatacc agatagtcgg ccccgcgcag
     1021 ttcatcagca atgtcttcta taggtttcaa tcccttgacg attgcaggag tcccgtctgg
     1081 caatgaaact ttccaaacga ggctggaaaa ggtgtccgca atgagaacag gttgcgaaac
     1141 gtgccaatga gcaggaaaaa caggcggcat gaacatcaac cccaagtcag agggtccaat
     1201 cgcagataga aggcaaggcg ttcgcggtcg ggggcttcga tccccaatac attgaatagg
     1261 acagcgaagg cgcgctctgc ttcatctggc gctgcccagt tctcttcggc gttagcaatc
     1321 atgagtgcca aatcggcata gcgatctgct gttccgagcc gcccaaggtc gatcagaccc
     1381 gtgcattgaa gagttttagg gtccaccatg aagttcggca tgcagggatc accatggcaa
     1441 acaaccatat cggtgcgctc ttggtcgagc cgcaccggta gctctcgttc gacacgagcc
     1501 aaaagatcga gctgcggcgt actcttgtcc tcgtccggta agaagtcggg attgacggca
     1561 ttgcgggaca ccacatcaac ggcgcgtccg aacattcgcg acagcctgcg ctcaaactga
     1621 cattgatcaa ccgataggct gtgaacagcg ccaagttgct gccccattga cggccacgct
     1681 ttgagcaaat ccgctccaga cagatcagcc gccggtactc ccggaattgc cgttatcacc
     1741 aagcatgcac cctcctgttc ctcctgccag ttgatgacct cggggcaagc cacacctcga
     1801 cctttgagcc aaatgaggcg gtcacgctct ccagcgagct caccgcggcg ggaagcaggt
     1861 gcgattttcg cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc agattctccg
     1921 cctctgacag gcaaccagtc agaatgcgat tcaccaaaaa aaatattagt tcgattcaat
     1981 ggaggttcct tcagttttct gatgaagcgc gaatatagag aaatatcccg aatgtgcagt
     2041 taacgaattc ttgcggtttc tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg
     2101 cgcggctcgt gtgtgcggat gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg
     2161 agtgtcgcgg ccccgacatc ccccggacca cggcctgtga gcgcgcgcag aaaggatttg
     2221 cgcgaaacag acagaagcac cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc
     2281 agcaccgaga gcgaggtttc gggagcagcc cccagaaaaa accccatgcc gggatcaagg
     2341 acaaggcggt tgcgtttgat accggcaccc gtcagcgccg cgatgcgcgc gtcaaagaac
     2401 gccgcaatgt gatccatgat gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct
     2461 tgcaccgaat gcataacgac gagtttggca gatgatttcg ccaattgcgg atagaacgca
     2521 gcgtctggaa aaccgcgaat atcattgaga taggccacac cacgcgacaa ggcataggct
     2581 tgcgtcgcgg gttgataact gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc
     2641 agcaccggcg cgatacgcgc gatttctgtg tcggacgaaa caggcgcggc gtcgggattg
     2701 ctggatgccg gaccgaggtc gatcacatct gccccctcgg ccatcagctt acgcgcctgc
     2761 gcaatggctg cgtctggcgc cagataccgg cctccatcgg agaaactgtc cgaggttatg
     2821 ttgacgatgc cgaaaatgat gagcgattta ttcatggggg cttctataat aataataatc
     2881 gagcatgagt ctcatacgga tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt
     2941 tgcggtgatc cgtttcatga tgatgtatct gtagcatttt ggaaataaat atatcatctc
     3001 tcaataagta gatatattta tcttttttgc ttctttggtg ctgcaagtca ccaatacgag
     3061 ggaaacctgt tacggagcgg tttaaccgta atcaacagtg actggtgtca tgttcggaag
     3121 attaattgat agtaatctac aaaggatctt cttgagatcc ttttcaatga atcgtaatct
     3181 cttgctctgt aaacgaaaaa accgcctggg gaggcggttt gttcgaaggt taagtcagtt
     3241 ggggaactgc ttaacccggt aacagggttc acgagaccgc cattaccaaa actgttcttt
     3301 cagtgtagcc gcagtcaggc ctctacttca agacctctta atctatcttc tcgtgcactc
     3361 ctgttactaa tggctgctgc cagtggcgtt ttgtcgtgtc tttccgggtt ggactcaagt
     3421 tgatagttac cggacaaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     3481 agcttggagc gaactgcctt cacggaactg agtgtcaggc gtggaatgag aaaccgcggc
     3541 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
     3601 ctaccagggg gaaacgcctg ggatctttat agtcctgtcg ggtttcgcca ccactgattt
     3661 gagcgtcaga ttttgcgatg cttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc
     3721 gtggccttat ctcttctttg ctaagtgtct tcctggtatc ttccaggaaa tttccgtccc
     3781 cgtccgtaag ccggtaccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc
     3841 gaggaagcgg aatatctcct gtatcgcata ttctgctgac gcgccggtgc tgcctttttt
     3901 ctcctgccac atgaagcact tcactgattt tcgcaatcgt ggcaacatag taagccagta
     3961 tacactccgc tagcgctaca tgactggttc agggctgcgc cccgaaaccc gctaacaacc
     4021 tctcagctac tggatatcac cccatgccat ctgaatatga tgaacaactg gattcgactg
     4081 atacgcagct tcccggcgaa tatgaccttt ttgatttaat ggcgcaggtt actgaagaga
     4141 acctgcatgc ccctgtggat tttgggtcgc ctgtcggccg ggaagcgtta tagctttttc
     4201 gcttgcccgc gtacgtggta atactccggt attacctttt atttctttat tttcatgtaa
     4261 ttacagtgga tttttaatac ccgggtatta ctcgtgaaaa cgaaatattc cttttttttc
     4321 ttttaattca tgcagttgcg atgaaaatcg cggctgcgtt aggtgtatga ccattttgag
     4381 gttgaaaaat aatcaaccat ggcgggtgat ttacgcaggt taaggggaga tcgtgacaaa
     4441 aaaagcgttc ctcactgtat cgttcatgcc tgcatgaacg ggttattctt cctgactaca
     4501 gaattacatc gctccttcag ttcatacatc acttttacat cacttttaca tcacttatac
     4561 atcactttta catcattcct tcagctcata cgtcactttc atcctctgtg ccatttgtac
     4621 atcaccccct tcctgaacgt gctgacagcg ggttttccgg ctgcgccggc aacgcttcac
     4681 ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tgggaagaac gccggaaaca
     4741 ggacttaccc cggctgaatg gtgtgaattt ccgcgctatg cacttgtgcg catactcatg
     4801 catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt
     4861 tttaccggtt tcccgtcaga aacgccctga gggcctctca ggcggtgctg ataacagatg
     4921 ttatggtaaa tcagagtcac ctgcactgtt actctgaacc ggagcgaaac agccgcgacc
     4981 agaatccccg ctttttttcc tgtgccagcg cgtcagccag acggctaatt tcggcctgta
     5041 gttgttccct ctcctcattt cgcaggcggt tttctgcttc ccgcgccatc ttgtcttcaa
     5101 gcattagggt aacagtctgc tgcagagcgt ccagtttctg ctgaatgacg tctagctgag
     5161 cattttccgc ctggctaacg acagatttgg agtgtccctc cttgtgcctt tttggtgtct
     5221 cagaagtgcg cagctcgcca taggcccgaa tcagttcact ggtctcgaac tcccggcgat
     5281 cattttttcc tgtgcgatag ctgacaaggc ctttagccat gtcacgatat aattgcgagc
     5341 gcgactttcc tgttaatttc agtgtctctt gaactgtgtg ccatgccata aaagtgacag
     5401 tgtctcctgt gtgtaccgta ttgtaacagc agaacatatt actgttttaa cacaaaaggt
     5461 ctgaggctgc ccgtgaggcg tgaaaaatac acaaaaaacc tgttgacggt gtggttttga
     5521 cgggcgtagt atttcgaata ctgtatataa acacattgtt ataaatacag tttttgctgt
     5581 ttttaaaagt caaagaggat aattttatga gtacagagtt aaatagcggt actgtcggtg
     5641 gttttggtca catgggacca aatgacccct acaatgatgg tcatagcggc tctggaagtt
     5701 cgggaggtag ttttaacggt ggtggtaatg gtggccatga taatagtagt ggttcatctt
     5761 atactgcatc ctttggtctc atcactggcc gacacccgat aactgtccca ggtacgaaga
     5821 atattaattc tgtcgatctt tctgctgtca ttaataacat tatacatcat gaaggtgcac
     5881 gcaataacat gtacctagat actgcgaaca atgtaagagt gggagtgacg ggcactggct
     5941 ggcaatgtct agcaacggca ggcatttcgg ctgagggtaa aagaactttc cgctaagcga
     6001 tagactgtat gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccagg
     6061 acggccagcc gggatcggga tactggtcgt taccagagcc accgacccga gcaaaccctt
     6121 ctctatcaga tcgttgacga gtattacccg gcattcgctg cgcttatggc agagcaggga
     6181 aaggaattgc cgggctatgt gcaacgggaa tttgaagaat ttctccaaag cccgcgacgc
     6241 agcgccggca ggcagagcaa gtagagggca gcgcctgcaa tccatgccca cccgttccac
     6301 gttgttatag aagccgcata gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg
     6361 ctggtgagcg ccgccagtga gccttgcagc tgcccctgac gttcctcatc cacctgcctg
     6421 gacaacattg cttgcagcgc cggcattccg atgccacccg aagcaagcag gaccatgatc
     6481 gggaacgcca tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca
     6541 atcattccga gcatgagtgc ccgcctttcg ccgagccggg cggctacagg gccggtgatc
     6601 attgcctggg cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc
     6661 gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg
     6721 acaagttgca tgatgaagaa gaccgccatc agggcggcga cgacggtcat gccccgggcc
     6781 caccggaacg aagcgagcgg gttgagagcc tcccggcgta acggccggcg ttcgcctttg
     6841 tgcgactccg gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa ggctgccgcg
     6901 gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc caccgagcac aggtcccgcg
     6961 accatcccga acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg ctcatcgcca
     7021 tcagtgatat cggcaatata agcgccggct accgccccag tcgccccggt gatgccggcc
     7081 acgatccgcc cgatatagag aacccaaagg aaaggcgccg tcgccatgat ggcgtagtcg
     7141 acagcagcgc cggccagcga gacgagcaag accggccgcc gcccgaaacg atccgacagc
     7201 gcgcccagca caggtgcgca ggcaaattgc atcaacgcat acagcgccag cagaatgcca
     7261 tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc
     7321 ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt
     7381 ctgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg aacgcgcgga
     7441 ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa gtgtcaagca
     7501 tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg aacgaggtcg
     7561 gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag cagccggcgc
     7621 tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa gccatgctgg
     7681 cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca tttctgatcg
     7741 ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg
     7801 ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt cgcttcctct
     7861 gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc agctacttca
     7921 ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag cgcggcggca
     7981 ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc ttcgacgaag
     8041 ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga ttggcgaaaa
     8101 ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga tcaggaccgc
     8161 tgccggagcg caacccactc actacagcag agccatgtag acaacatccc ctcccccttt
     8221 ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct gccctagcgt
     8281 ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctcct gc
//
LOCUS       DAJVXP010000158         8254 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVXP010000158 DAJVXP010000000
VERSION     DAJVXP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.158"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            406..1281
                     /locus_tag="OQS10_003581"
     CDS             406..1281
                     /locus_tag="OQS10_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS2294520.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1289..2065
                     /locus_tag="OQS10_003582"
     CDS             1289..2065
                     /locus_tag="OQS10_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS2294521.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2234..4495
                     /locus_tag="OQS10_003583"
     CDS             2234..4495
                     /locus_tag="OQS10_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS2294522.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4564..5739
                     /gene="senB"
                     /locus_tag="OQS10_003584"
     CDS             4564..5739
                     /gene="senB"
                     /locus_tag="OQS10_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS2294523.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6265..6726)
                     /locus_tag="OQS10_003585"
                     /pseudo
     CDS             complement(6265..6726)
                     /locus_tag="OQS10_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6764..>8254)
                     /locus_tag="OQS10_003586"
     CDS             complement(6764..>8254)
                     /locus_tag="OQS10_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS2294524.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALL
                     YSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         2181 a         2034 c         1975 g         2064 t
ORIGIN      
        1 tttcgcacag tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct
       61 caccccccga taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga
      121 catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg
      181 atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat
      241 agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat
      301 tctttttgtt ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat
      361 gttttgtcaa taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc
      421 cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc
      481 ataaagcagg atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg
      541 cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat
      601 cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt
      661 caaaatttgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag
      721 caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat
      781 agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac
      841 atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag
      901 cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct
      961 gtggtacaag aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc
     1021 cagcaactga cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag
     1081 cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc
     1141 atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg
     1201 cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg
     1261 ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt
     1321 aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt
     1381 tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca
     1441 atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca
     1501 acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt
     1561 cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga
     1621 aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac
     1681 agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc
     1741 ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa
     1801 aggccaccta aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg
     1861 cacagccgtc gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga
     1921 aatctcttca ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca
     1981 accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc
     2041 gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa
     2101 atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca
     2161 aaaagacagt agaacataat taataaacaa atagttatac ttatctttat taatattata
     2221 cccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag
     2281 ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc
     2341 agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga
     2401 gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac
     2461 agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt
     2521 gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca
     2581 gagtttttac ggaacctcta cctccggaac aacgacgcat ggttcaacta acaatatggc
     2641 tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag
     2701 tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga
     2761 cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa
     2821 tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac
     2881 gggaagcatt ggcgttatgg ctgctgtgag cggcagttct gtgtactcta atttctcaaa
     2941 tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa
     3001 atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc
     3061 tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa
     3121 atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg
     3181 cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa
     3241 tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgacct
     3301 ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc
     3361 ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg
     3421 ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc
     3481 agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc
     3541 acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa
     3601 cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag
     3661 caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc
     3721 catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga
     3781 taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag
     3841 caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg
     3901 cagtatgcct gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa
     3961 tatgtacatc tacgttaact cggcaagcga cgttatcgca aagggcttcg aactggagat
     4021 ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca
     4081 gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa
     4141 atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat
     4201 cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac
     4261 cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac
     4321 ttacgagtac aaccgcaacc taacactgaa actttcggta caaaacctga tgaacagaga
     4381 ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg agacccaccc
     4441 agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca
     4501 atttccgcta ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga
     4561 aaaatgaata ttttcacttt atccaaagca ccgctatacc tgttaatttc actattttta
     4621 cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa
     4681 acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt
     4741 aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg
     4801 tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca
     4861 ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg
     4921 tccctgcgta tcagtatgtt tgacacccca accggcaaac tggtaagaac cagcgacagg
     4981 ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcattttcg ccatatggtt
     5041 tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag
     5101 ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc
     5161 gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag
     5221 aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg
     5281 ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg
     5341 gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt
     5401 acaaaagatg gactctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac
     5461 acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc
     5521 aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa
     5581 tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac
     5641 attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct
     5701 aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cggggggaaa aaacatggga
     5761 taaaaactcc gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg
     5821 ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca
     5881 cagcattgta cgatcaactg atttgtgcca ataattaaat gaaatcacag ctatgcctgt
     5941 tcatggggtt ctaggccgga accccagatt tttccgattg tttgttttct aactgattat
     6001 tcagtagttt tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac
     6061 tatgataaaa gtcaaagtag gggtgagttt ggatatggaa attattgtac tataaggaga
     6121 gcatgaaaac ccctggggca gtcactgaat gagggaccag cagcggtaaa ataaccagca
     6181 taatttctgc atggtcgatt tttataccgt gacaggctgt tttttatact tggggtcagt
     6241 ttggatatcg aaaattattg tacgttaagg ttgttttttg accttgtcga tcttaactga
     6301 tctcaaacga cgaaacttac ctgaagtcgg tatccggctg tttttccaga tataatcgcc
     6361 tgtcagattg atatgttccc aacctaatgg tgataaatgc gataatagtt gagcatttat
     6421 tttcatgccc ttacgcttca gagcatcaac cgctctttcc atgtaaactg tgttccataa
     6481 ggaaatagcc gccgtcaata aagtcaatcc actggcgcga taactttgat tttccggttt
     6541 ccgatctcta atctccccca atcgatgcat gaatacagca cgggccagag cattacgggc
     6601 ttctcctttg ttcagcccgg cctggacacg tcgccgcaat gccggatccc gaaaccagtc
     6661 cagcataaac agtgttcgtt ctatacggcc aatctctctc agagctttag ccaacccatt
     6721 ttgtttgtaa gcgtgcagca agaaccgtat tgacgggaat gtgttattca gtcggcagtg
     6781 ctacgcgcca ggggagcagt tcgccgaccc ggtttatcgg ccagtcggct atgacgtcaa
     6841 ggacatagcg gaggtagctt tctggctcca ctccgttcag tttgcacgtc ccgatcaggc
     6901 tgtacagcag cgctccccgc tctcctccat gatccgaacc gaagaacagg tagtttttgc
     6961 ggcccagact gaccatccgc aacgcatttt cagcgatgtt attgtccgcc tcagcccagc
     7021 catcatctgc atagtacgtc agcgccggcc actggttcag ggcgtatgcg aacgctttcg
     7081 ccagttctga gtgtcgcgac agggttttca tcttttcacg cagccagctt tccagggatt
     7141 tcagcagcgg tttcgttttc aactgacgtt cggcaaggcg ctgttccgcc gtcattcccc
     7201 ttatctctgc ctcgatggcg tacagttcgc cgatccgttt cagcgcttcc tccgtcaggg
     7261 ctgacggggt gcgaacgtgc acatcgtgga ttttacggcg ggcgtgagcc caacaggcgg
     7321 cttccgttat ccggccatcc cggtacagct cgttgaaccc ggcgtatgca tccgcctgca
     7381 gtacaccact gaaccccgca agatgggtct gcggatggat gccttttctg tccgggctgt
     7441 aagcgaacca caccgccggc gccagcgttg acccggcgtt acggtcgtca cgaacatacg
     7501 cccacaaccg cccggtcttc gttttcttat tgcctggcaa cagcaccggg acaggcgtgt
     7561 catcaacatg gagcttaccg tcagtcagca catagccatg aagcgcctct tccatcggag
     7621 acagcagccg gcagcatgca tccacccagc ccgacagcag tgaacggctc agctccacac
     7681 cttgccggcc gtatatttct gactggcgat acagcggggt gtgctctgca tacttcgagg
     7741 tcagcacgcg ggccagcagc cccggtccgg cgataccccg ctcgatgggc cgcgaaggtg
     7801 caggtgcctg cacgatggca tcgcactgag tacaggcatg tttttcccgt accgtccgga
     7861 taacccggaa ggcgctacgc atcaactcca gctgttcggc ggtatcctcg cccagatagc
     7921 tcagtgaacc gccgcagttc gggcagcacg gcgccgcagg caacagtcgc ttttcgtcac
     7981 ggggtagtga ttcagggaac ggcttacggg tgcgggtctg acgcaacgga cgctgtactg
     8041 ccgggtcata caccctacca gtcagcgtat cgctctcttt ctgaagccgg ttcagatcgg
     8101 cttccatttg tgcgatacgg cgggagactt tttcggaacg actgccgaag ttcatccggc
     8161 ggagtttatc cagctgcgcc tgcagatggt ctatttcgcg ctcccggttg ctaagctttt
     8221 cctgcagggc gtggatcagc gcttcctgtt cggc
//
LOCUS       DAJVXP010000159         8238 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVXP010000159 DAJVXP010000000
VERSION     DAJVXP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.159"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            18..902
                     /locus_tag="OQS10_003587"
     CDS             18..902
                     /locus_tag="OQS10_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS2294525.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(953..1807)
                     /gene="yeaE"
                     /locus_tag="OQS10_003588"
     CDS             complement(953..1807)
                     /gene="yeaE"
                     /locus_tag="OQS10_003588"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS2294526.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1897..2643)
                     /gene="mipA"
                     /locus_tag="OQS10_003589"
     CDS             complement(1897..2643)
                     /gene="mipA"
                     /locus_tag="OQS10_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS2294527.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3079..5013
                     /gene="yeaG"
                     /locus_tag="OQS10_003590"
     CDS             3079..5013
                     /gene="yeaG"
                     /locus_tag="OQS10_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS2294528.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5126..6409
                     /locus_tag="OQS10_003591"
     CDS             5126..6409
                     /locus_tag="OQS10_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCS2294529.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6556..8031
                     /locus_tag="OQS10_003592"
     CDS             6556..8031
                     /locus_tag="OQS10_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS2294530.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2185 a         1973 c         1909 g         2171 t
ORIGIN      
        1 agttaaagga ctgcgtcatg attaagaaaa tttttgccct tccggtcatc gaacaaatct
       61 cccctgtcct ctcccgtcgt aaactggatg aactggacct cattgtggtc gatcatcccc
      121 aagtaaaagc ctcttttgca ttacagggcg cacaccttct ctcgtggaaa cctgcgggtg
      181 aagaagaagt tctgtggttg agcaacaaca caccgttcaa aaatggcgtc gctattcgcg
      241 gtggcgtacc ggtttgctgg ccgtggtttg gtccggcggc acaacaaggt ctgcctgcgc
      301 acggttttgc ccgcaacctg ccgtggacgc tgaaatcaca taatgaagat gctgatggcg
      361 tagcgctgac ttttgaattg acgcaaagcg aagagacgaa aaaattctgt ccgcacgact
      421 ttacgctgtt agcgcatttc cgcgtgggta aaacctgtga aatcgatctt gaatcacatg
      481 gcgaatttga aaccacctct gccctgcata cctactttaa cgtgggtgat atcgctaagg
      541 taagcgtcag tgggctgggc gatcgcttca ttgataaagt gaatgacgcg aaagaagatg
      601 tactgaccga cggtattcag accttccctg accgtaccga tcgcgtgtat ctgaatccgc
      661 aagattgcag cgtgattaat gatgaagcgc tgaatcgtat tatcgccgta ggccaccagc
      721 atcatctgaa cgttgtaggc tggaacccgg gaccggcgct ttcaattagc atgggcgata
      781 tgccggatga tggctacaaa acatttgttt gtgtagaaac ggcttacgct tcagaaacgc
      841 aaaaagtcac caaagagaaa cctgcacatc tggcgcaatc cattcgcgtt gcgaaacgtt
      901 aatttgcgtt aatgttgtgt gccgggtgcg atgcatccgg cacgtatcaa catcacacca
      961 tatccagcgc agtttttcct tttggtgccg gatacgcttt atccagcatg gttaattccg
     1021 ctgacgaaag ttcgacctca agcacagccg cattctgttg aacatgggca atcgtggccg
     1081 cttttggaat cgccatcaca ccctgatggc tgattaccca cgccaacaat acttgtgccg
     1141 agctgatatt gtgagcatgt gcaatttcgt tgactaccgc gttttttaac agtccattgc
     1201 gcaaccgccc ggcctgggct aatggactgt aagccatcac cggcatctgc tgttgctggc
     1261 accaggggag tagatcgtac tcaatccctc gtgaaccgag atggtaaagc acctgattag
     1321 tggcacactg atttcccccc ggcagctgcc agagttcctg catatcagca taatcaaggt
     1381 tagaaacgcc ccagcggcgg atttttccct gggcgatcaa tttttccatc gctgcaacag
     1441 tctcttcaaa agcgaaactg ccagacctgt gtaataagta gagatcgaga taatcagtat
     1501 tgagacggcg taaactggct tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg
     1561 gatagacttt agagacgaga aagacgttat cacgcagacc ggttaatgct tccccaacca
     1621 ccttttcggc accgccatcg gcatacattt cggcggtatc aatgagggtt aaaccgagct
     1681 caatgcccgc gcgtagtgca gcaacttctg ttttgcgctg actggcatct tcgcccatat
     1741 accatgttcc ctgccctatg gctggcagtg agacatcgcc actaaattga atcatttttt
     1801 gttgcattgt ttcctcccag gtattgcacc accgtaatgc aaaacagggc gtgacgccct
     1861 gtttttatgc acaaaatgcc ctggaaagat gcattatcag aatttgtagg tgatcccggt
     1921 agaaatcagg ccagtccagg atttatccac catcgggctg tcagtaactt catcagacag
     1981 acgggtatag cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt agctggcgct
     2041 cagctccagg taagggctcc agctgtcgtt cgggttatag ccacgcagac cgctgcgagc
     2101 ggactctttg cgcgatacgc cataatagta ttcgttctgg ttttcgctgt tccactgcac
     2161 accgataccc ggggtcactg tcaggccgcc gttggtgtaa cgatacaacc aggccatatc
     2221 ccagacaatg ccgttgctgt tatccagggt atcgccagtc agggtggtac gcaggtaacc
     2281 gtactgggta aagtgagcat aagacagacc agccatcatg gtgctcttac ggtcatccag
     2341 gtgacgcatt tggtgatcgc cactgtcttt cgctttgaag taaagcggcg accaataagc
     2401 ggtaattgaa agtttgtccg ttgcgtcatt ccacaggtag taaccaccac ctaagccacg
     2461 gaaccagaag ttatcgcctt catagttgat taccggtact gggtaaacat cggtatcgta
     2521 atctttatat gggtgttcaa cgacaccgac gcctgcgccc agggaaaatt taccttcagc
     2581 gtgcgctacg cctgcagacg ttgcgataag cactccaagt gccagaagtt tgagtttggt
     2641 cacaattaat cattccttaa acaaatgttt agcgggcgac aaagtttacc cgtcaataca
     2701 tcgaaaccca acctttttac gttttcattt tttaaagtaa ctgtttaatt ttcctgacgc
     2761 ggatgacacc gcgcttacag ccaaatgaat ttagcgttac tggcgagcct tgtctttaca
     2821 ttaattatgc aaaatttatg gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg
     2881 caggcaagtt ttccatttgc catctacgct taattttgaa ggtgtatcac cgggcacgtt
     2941 gttctcatcg tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca
     3001 gtttacctct tccgggagcc tctactattc atatgaacgg ctcttaacct gtgctaaaaa
     3061 acgaaaggac ggcataccat gaatatattc gatcactatc gccagcgata tgaagctgcc
     3121 aaggacgaag agttcacact gcaggagttt cttaccactt gtcggcaaga tcgcagtgct
     3181 tatgccaacg cggctgagcg gctattgatg gctatcggtg agcctgtcat ggtcgataca
     3241 gcccaggaac ccagactttc tcgactcttt tctaaccggg tcattgcacg ttatccggca
     3301 tttgaagagt tttacggcat ggaagacgcg attgaacaga ttgtctctta tctgaaacac
     3361 gcggctcagg ggctggaaga gaagaaacaa atcctgtatc tgctggggcc tgtgggtggg
     3421 ggtaaatcat cgcttgctga gcgactgaaa tcattaatgc agctcgtacc gatttatgta
     3481 ttgagcgcca acggtgagcg tagcccggtc aacgatcatc cgttctgtct tttcaatccg
     3541 caggaagatg cgcagattct ggaaaaagag tatggcattc ctcgccgcta tctcggcacc
     3601 atcatgtcgc cgtgggcggc aaaacgcctg catgaatttg gtggcgatat cactaagttc
     3661 cgggtagtga aggtctggcc gtcaattctg caacaaattg ctatcgccaa aacagaaccc
     3721 ggtgatgaga acaaccagga catctccgcg ctggtaggga aagtcgatat tcgtaaactc
     3781 gaacactacg cgcagaatga cccggacgcc tacggctatt ccggtgcgct gtgccgcgcc
     3841 aatcagggga tcatggaatt tgtcgagatg tttaaagcac cgattaaagt gctgcatccg
     3901 ctgttaaccg ctactcagga aggtaactac aacgggacgg aaggtatctc cgccctgccg
     3961 ttcaacggga ttattctcgc ccactcgaac gaatccgaat gggtcacttt ccgtaataac
     4021 aaaaacaacg aagccttcct cgaccgtgtt tacatcgtga aggtgccgta ttgcttacgc
     4081 atttccgaag agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc
     4141 ccatgcgccc ctggcacgct ggaaacactg tcacgttttt ccattctttc gcgcctgaaa
     4201 gagccagaaa actccagcat ttattcaaag atgcgggttt atgatggcga aagtctgaaa
     4261 gacaccgatc ccaaagccaa gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa
     4321 gggatgaaag gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc
     4381 gatcatgtag aagtggcggc aaacccggtc catctgttct acgtcctaga acagcagatc
     4441 gagcgcgagc agttcccgca agagcaggca gaacgctatc tggagttcct gaaaggttat
     4501 ctgatcccga aatatgccga gtttatcggc aaagagatcc agacggccta ccttgaatcc
     4561 tattccgaat atgggcaaaa cattttcgac cgttatgtta cctacgcgga tttctggatt
     4621 caggatcagg agtatcgcga tccggatacc gggcagctgt ttgaccgcga gtctcttaac
     4681 gccgagctgg agaaaatcga gaaaccggcg gggatcagta atccaaaaga tttccgcaac
     4741 gagattgtta acttcgtact gcgcgccaga gcgaataaca gcggacgcaa tccgaactgg
     4801 accagctatg aaaaactgcg cacggttatc gagaagaaaa tgttctccaa taccgaggag
     4861 ctgttgccgg ttatctcgtt taacgccaaa acgtcaaccg acgagcagaa gaaacacgac
     4921 gactttgtcg accgtatgat ggaaaaaggc tacacccgta aacaggtgcg tttactgtgc
     4981 gaatggtatt tgcgcgtacg taaatcgtct taacaaccct ggcccggtac acatgctacc
     5041 gggcctataa cgacagcgaa ccgtgggcca gagaaatggc aacacaggcg tagcatacag
     5101 ttggcaaatg tagtacgggg ggcatatgac cgggtttatt gaccggcgtc tgaacggcaa
     5161 aaacaaaagc atggtgaatc gccagcgttt tttacgccgt tataaagcgc aaattaaaca
     5221 gtcgatctcc gaggccatta ataagcgttc ggtgactgac gtcgacagcg gcgaatccgt
     5281 atccattccc acggaagata ttagcgaacc gatgtttcat caggggcgtg gcggcctgcg
     5341 ccaccgcgta catccgggca atgaccattt cgtccagaac gaccgaattg aacgtcccca
     5401 gggtggcggc ggaggttccg gcagtggtca gggccaggcc agccaggatg gtgaaggtca
     5461 ggatgaattt gtctttcaga tttcgaaaga tgagtatctt gatctgctct ttgaagattt
     5521 ggccttaccg aatctgaaac aaaaccaaca acgccagctg accgaatata aaacgcatcg
     5581 ggcgggttat accgctaacg gcgttccagc caatatcagc gttgtgcgtt ccttgcagaa
     5641 ctcactggcg cgacgcacag ccatgacggc gggcaagcga cgggaacttc atgcgctgga
     5701 agagaatttg gccatcatca gcaacagtga acctgcgcaa ctgctggaag aggaacgtct
     5761 gcgcaaagaa attgcagaat tacgtgccaa aattgaacgc gtccctttta ttgacacctt
     5821 cgatttacgt tacaagaact acgagaagcg gcccgatccc tccagccagg cagtgatgtt
     5881 ttgcctgatg gacgtttccg gttcaatgga tcaatccact aaagatatgg ctaagcgttt
     5941 ttatattctg ctgtatctgt tcctcagcag aacgtataag aacgtggaag tcgtatacat
     6001 ccgccatcat acccaggcga aagaagtcga tgaacatgag tttttctact cgcaggaaac
     6061 aggcggcacc attgtttcca gcgccctgaa actgatggat gaggtagtga aagagcgtta
     6121 taacccggca cagtggaata tttacgctgc acaagcatcg gacggcgata actgggccga
     6181 tgactctccg ctttgccatg aaatcctggc gaaaaaatta ttacctgttg ttcgttatta
     6241 cagctatatc gaaattaccc gtcgtgcaca tcagacattg tggcgagaat atgagcatct
     6301 gcaatctact ttcgacaact ttgcgatgca gcacatccgc gaccaggatg atatttatcc
     6361 ggtgttccgt gaactgtttc ataaacaaaa tgcaacagct aaagactaaa actatcagcc
     6421 aggtcattat cgcctggctg atttttagct tactgtaaat tatctcattt attacataca
     6481 ttagcttaca atcgctttaa atatgacagc ataaccttta cataatttag ttccagaaaa
     6541 caatcattcg gaaaaatgat tcagtcaaca cgtatttcca tggggttatt ctttaaatat
     6601 tttttatcgt taacgaaaat tgatcctggt caaaactata tatctctgcc atcaataaaa
     6661 tccagcactc acattgctct cctttttatg gtttctatgg gtacacaaaa attaaaagct
     6721 caaagctttt ttattttcag tttattgctg acgttaattt tattttgcat tactacctta
     6781 tataacgaaa acacaaatgt aaaactcatc ccacagatga attacctgat ggttgttgtg
     6841 gctttgtttt tccttaacgc cgtcattttt cttttcatgt taatgaaata tttcactaac
     6901 aaacaaattt taccaacact cattttaagc cttgcatttt taagtggcct tatctattta
     6961 gttgaaacca ttgtaattat ccataaacca attaacggca gtacactgat ccagacaaag
     7021 tcgaatgatg tttctatttt ctatattttc cgccaactca gttttatttg tttaacctcg
     7081 ctggcactct tttgttatgg aaaagacaac atccttgaca acaataagaa aaaaacggga
     7141 atcctgttgc tggcgctgat tcctttttta gtttttcccc ttctggcaca caatctgagc
     7201 agttataacg ctgactattc tttgtatgtc gtcgactact gtccggacaa ccatactgcg
     7261 acctggggaa tcaactatac aaaaatattg gtttgtctgt gggcattttt actgttcttt
     7321 attatcatgc gtacacgatt agccagcgaa ctatggccgt taatagcatt attatgtctg
     7381 gcatcgctat gctgcaactt acttctactg actctggatg agtataatta caccatctgg
     7441 tatatcagtc gcgggattga agtttccagt aaactgtttg ttgtgtcttt tctgatttat
     7501 aacatttttc agaaactgca actctccagc aaactggcag ttcatgatgt gctgaccaat
     7561 atttataatc ggcgctactt tttcaacagc gtagagtcat tattgtcgag acctgttgtt
     7621 aaggacttct gtgtcatgct ggttgatatt aatcagttca aacgcatcaa tgcccaatgg
     7681 ggacatcgtg tgggtgataa agtgctggtt tcaattgtcg atattatcca gcaaagcatc
     7741 cgccccgatg atattttagc gcgactggag ggtgaggtgt ttggcttgct atttaccgaa
     7801 ctcaatagtg cccaggcaaa aatcattgcg gaacgtatgc gtaaaaatgt cgaactcctg
     7861 accggcttta gtaacagata tgatgttcct gaacaaatga ccatcagtat tggcacggtt
     7921 ttttcaacgg gtgacacgcg taatatctcg cttgtcatga cggaagcaga taaagcctta
     7981 cgcgaagcga aaagcgaggg gggcaacaaa gtgattattc atcatattta agtgcaaaaa
     8041 tattcagagc catgcttttt gcgtggcttt tgcatacaat ttattactat tacccctaaa
     8101 tttcccctcc ccagcggcgc agagatgagt ataattagcg cccctgtgcc aggccgcaat
     8161 cgaactttat ctggttttct cgtttcacta accgaaggag tgccatttat catgaaattg
     8221 caccatagaa tgctccgg
//
LOCUS       DAJVXP010000160         8183 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVXP010000160 DAJVXP010000000
VERSION     DAJVXP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.160"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OQS10_003593"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OQS10_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS2294531.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OQS10_003594"
     CDS             complement(2975..3697)
                     /locus_tag="OQS10_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS2294532.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OQS10_003595"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OQS10_003595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS2294533.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYYTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8183
                     /locus_tag="OQS10_003596"
     CDS             6679..>8183
                     /locus_tag="OQS10_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCS2294534.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNAA"
BASE COUNT         1979 a         2031 c         2320 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta ttacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cgc
//
LOCUS       DAJVXP010000161         8104 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVXP010000161 DAJVXP010000000
VERSION     DAJVXP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.161"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(101..796)
                     /locus_tag="OQS10_003597"
     CDS             complement(101..796)
                     /locus_tag="OQS10_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS2294535.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OQS10_003598"
     CDS             complement(789..2216)
                     /locus_tag="OQS10_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS2294536.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OQS10_003599"
     CDS             complement(2227..2946)
                     /locus_tag="OQS10_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS2294537.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OQS10_003600"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OQS10_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCS2294538.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OQS10_003601"
     CDS             4554..5879
                     /locus_tag="OQS10_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCS2294539.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OQS10_003602"
     CDS             5988..6224
                     /locus_tag="OQS10_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS2294540.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OQS10_003603"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OQS10_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCS2294541.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OQS10_003604"
     CDS             complement(6989..7858)
                     /locus_tag="OQS10_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS2294542.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAJVXP010000162         8003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVXP010000162 DAJVXP010000000
VERSION     DAJVXP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.162"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..1133)
                     /locus_tag="OQS10_003605"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="OQS10_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="OQS10_003606"
     CDS             complement(1133..1315)
                     /locus_tag="OQS10_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294543.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="OQS10_003607"
     CDS             complement(1327..3060)
                     /locus_tag="OQS10_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS2294544.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="OQS10_003608"
     CDS             complement(3057..3551)
                     /locus_tag="OQS10_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS2294545.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="OQS10_003609"
     CDS             complement(3673..4023)
                     /locus_tag="OQS10_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS2294546.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="OQS10_003610"
     CDS             complement(4066..4530)
                     /locus_tag="OQS10_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS2294547.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="OQS10_003611"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="OQS10_003611"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCS2294548.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="OQS10_003612"
     CDS             complement(5024..5239)
                     /locus_tag="OQS10_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS2294549.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(5920..5994)
                     /locus_tag="OQS10_003613"
     tRNA            complement(5920..5994)
                     /locus_tag="OQS10_003613"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5959..5961),aa:Gly,seq:tcc)
     gene            complement(5998..6073)
                     /locus_tag="OQS10_003614"
     tRNA            complement(5998..6073)
                     /locus_tag="OQS10_003614"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6038..6040),aa:Thr,seq:tgt)
     gene            complement(6076..6152)
                     /locus_tag="OQS10_003615"
     tRNA            complement(6076..6152)
                     /locus_tag="OQS10_003615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6116..6118),aa:Arg,seq:tct)
     gene            complement(6154..6230)
                     /locus_tag="OQS10_003616"
     tRNA            complement(6154..6230)
                     /locus_tag="OQS10_003616"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6194..6196),aa:Ile,seq:cat)
     gene            complement(6420..6983)
                     /locus_tag="OQS10_003617"
     CDS             complement(6420..6983)
                     /locus_tag="OQS10_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS2294550.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(7087..7305)
                     /locus_tag="OQS10_003618"
     CDS             complement(7087..7305)
                     /locus_tag="OQS10_003618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294551.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(7464..7850)
                     /locus_tag="OQS10_003619"
     CDS             complement(7464..7850)
                     /locus_tag="OQS10_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2294552.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(7840..>8003)
                     /locus_tag="OQS10_003620"
     CDS             complement(7840..>8003)
                     /locus_tag="OQS10_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294553.1"
                     /translation="DNILKAPLDALTHAGLLIDDEQFDEINIVRGQLVPGGRLGVKIY
                     EITGDNDGA"
BASE COUNT         1877 a         2254 c         1904 g         1968 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tcccgccccc cgacagacct gtgctatcgg aaagaaaaaa
     5281 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
     5341 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
     5401 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
     5461 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggggcatt tcattatttt
     5521 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
     5581 catgattcca gtccatgcaa aatctgattt ccagctcaca cataccgagc gctgtacaaa
     5641 aaaccgccag ccaaggcagt taagagtgtg ttcccggggt ttgcttagaa tattttgtaa
     5701 gttgtccgaa gagatattta caacaccaga atgatgaatc atcagtcccc tgccagaggt
     5761 tcactacgta acctgaccga caatgcacca ttctggtgct gaaaaaagag cactggaact
     5821 gcaacaaaac aacatcacag aacaggagat ggtaaggagt cagacattcc cacacaatat
     5881 tgtgtcagtg caaataacaa ccttcgtctc aaatctcgct ggagcgggca gcgggaatcg
     5941 aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc ccgcatatgg
     6001 tgccgactac cggaatcgaa ctggtgacct actgattaca agtcagttgc tctgcctgct
     6061 gagctaagtc ggcgctggcc caccaccgag gactcgaacc tcgcaccgtc aacttagaag
     6121 gttgatgctc tatccggatg agctagtggt ggttggtggc ccttgctgga cttgaaccag
     6181 cgacctggcg attatgagtc gctcgctctg accaactgag ctaaagggcc ggaggcagaa
     6241 taataaccat atgtcatcac atctgcaaac tcatctgacc accagcacgt ttaacgtcct
     6301 gtgccgtttt tcaggtataa aaaaacccgc tcatcggcgg gtttaagctg tgtggcgtag
     6361 taaccactct taacaggata ttcaactttt tacgatcgta aagttttcgg aaaaaatttt
     6421 taaaaccgca tcaaccgctc caccagttgc tctttacggg caacgatcca cccgtgttgc
     6481 tccagataaa atttaaaccg ttccagagta cataccatcg catcggcggg taccttttcc
     6541 gtgaactcaa cctgaccgtg tttatcgaag tggctcagta atgcgcatcc atcattttcg
     6601 atagatgtgt tttgtgccgc tggtggctgt ttctggttga aataacagtc ttccagtttt
     6661 tcgaacactt cccacgcctg atcggtttcg agcattttgg cgtgacgggc tgcgccacgt
     6721 tctgtccaga ggatgaggga gcgaacgtta cgggcaattt tcacagaaac tcttaaagag
     6781 ttttcgttct tcaactctcg taacgcatca ccttctatca ggaaaaaatg tttaccttct
     6841 ataaaccgtt ctttgtttcg attgaaattg tttgtcaaac gctggcgctc tgttccgtac
     6901 aaatcagcca gcagctctgt tgtcatgaca ggaagatggt tatgcgtaag tgacgggagt
     6961 ttttcaatag acgtgcgatt catagacatg tcctttcgaa tattttgatt tacccctttt
     7021 gagagggtga ccgggcgctc aaaaccgtcg aaagtcggcg ggcgtatttc cattgctggt
     7081 attgtattag ccgcacaccc ggtcataaac caagaattct ggacacaaaa aaaccacatg
     7141 gctatcgggt gtggataccg ctttcgaggt gttttgagca ccgcggggaa atgtaaactg
     7201 caaacctgaa actgtcaaca ccgtcaggtt ccaatcatcg ggtggtgaga cgcacagggt
     7261 tggaactacc gggaaaccga ccggcgagct tttcagctcc cccatgcgcc ccaccataat
     7321 tcagatgtgc gcgtgcatac gacaataaaa aacacgctcg cggcgtgtgt ctgtcgcggt
     7381 ctctatccgg ggttccaatc ccgacgccag attttgctgg cgcgtgagga atatagcccc
     7441 ggacaatgtg tctggtcaag cacctacata attcgttcta cgtatctgtc catctccagc
     7501 cggatatcaa gcatcatcaa catgccatca ataacccctt ccgctttctg caggcgcttg
     7561 ccaatacagg tatccgaaca cccatgtttc cgtgccagac tcataaaagt cattccacct
     7621 acgtaataat ccaccagcaa atcgtgcaaa tcctgatttt tcttgttcaa ccgggccata
     7681 cagccacaaa ttatcattgc atcatcatca gaacactgag ggcgtgattt cactttcgac
     7741 ggaattaacc ctttaaaacc agcagcgatc gacgcccagg atacatcttc atgagtgttt
     7801 gcagcccaag ccccccaccg ttccataacc tgctgaatat cacgcgccat cgttatcacc
     7861 tgtgatttca taaatcttca cgcccagtcg cccaccagga acgagctgac cgcgcacaat
     7921 attgatttca tcaaactgct cgtcgtctat gagtagtccg gcatgcgtca gcgcatccag
     7981 tggtgctttc aggatattgt cca
//
LOCUS       DAJVXP010000163         7968 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVXP010000163 DAJVXP010000000
VERSION     DAJVXP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.163"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..239
                     /locus_tag="OQS10_003621"
     CDS             <1..239
                     /locus_tag="OQS10_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCS2294554.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="OQS10_003622"
     CDS             279..1142
                     /locus_tag="OQS10_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS2294555.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="OQS10_003623"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="OQS10_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCS2294556.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="OQS10_003624"
     CDS             2679..4037
                     /locus_tag="OQS10_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS2294557.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="OQS10_003625"
     CDS             4180..5130
                     /locus_tag="OQS10_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCS2294558.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="OQS10_003626"
     CDS             5140..6522
                     /locus_tag="OQS10_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS2294559.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="OQS10_003627"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="OQS10_003627"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCS2294560.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAJVXP010000164         7936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVXP010000164 DAJVXP010000000
VERSION     DAJVXP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.164"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQS10_003628"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OQS10_003628"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS2294561.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="OQS10_003629"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="OQS10_003629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS2294562.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="OQS10_003630"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="OQS10_003630"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS2294563.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="OQS10_003631"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="OQS10_003631"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS2294564.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="OQS10_003632"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="OQS10_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS2294565.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="OQS10_003633"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="OQS10_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS2294566.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="OQS10_003634"
     CDS             5724..5885
                     /locus_tag="OQS10_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS2294567.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="OQS10_003635"
     CDS             6007..7080
                     /locus_tag="OQS10_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS2294568.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="OQS10_003636"
     CDS             7077..7859
                     /locus_tag="OQS10_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS2294569.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1895 a         2047 c         2152 g         1842 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc atatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gttttgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc
     2581 ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggatgc cggtaagact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggctcct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 agcgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt gatcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc ggtctggatc
     7381 tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtatacac gacaaactgg
     7501 cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagc
     7861 ctacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctac
//
LOCUS       DAJVXP010000165         7892 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVXP010000165 DAJVXP010000000
VERSION     DAJVXP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.165"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..3165
                     /locus_tag="OQS10_003637"
     CDS             <1..3165
                     /locus_tag="OQS10_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS2294570.1"
                     /translation="LRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITI
                     KGKTTSQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYP
                     NTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNN
                     MAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWL
                     TTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRY
                     SFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAH
                     RAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTR
                     TLTLDREITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGL
                     KLPTLHQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPP
                     AVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETT
                     YRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHL
                     AVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVN
                     TVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSI
                     TDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIG
                     AGIENTPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTIT
                     SGGNPSAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDF
                     VKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGN
                     NPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGN
                     QGAGNITDCTVIVTKKAASGISIR"
     gene            3236..3752
                     /locus_tag="OQS10_003638"
                     /pseudo
     CDS             3236..3752
                     /locus_tag="OQS10_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            3817..5221
                     /locus_tag="OQS10_003639"
                     /pseudo
     CDS             3817..5221
                     /locus_tag="OQS10_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            5170..5322
                     /locus_tag="OQS10_003640"
                     /pseudo
     CDS             5170..5322
                     /locus_tag="OQS10_003640"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5299..5478
                     /locus_tag="OQS10_003641"
                     /pseudo
     CDS             5299..5478
                     /locus_tag="OQS10_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            6193..6900
                     /locus_tag="OQS10_003642"
                     /pseudo
     CDS             6193..6900
                     /locus_tag="OQS10_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            6900..7481
                     /locus_tag="OQS10_003643"
     CDS             6900..7481
                     /locus_tag="OQS10_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2294571.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         2050 a         1952 c         2267 g         1623 t
ORIGIN      
        1 ctgcgtttta ccttcggtgt acaggcactg gtggaaacca cctcaaaggg agacaggaat
       61 ccgtctgaag ttcgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa
      121 gacatcacca ttaagggcaa aaccacctcg cagtatctgg cctcggtggt ggtggataac
      181 ctgccgccgc gcccgtttaa tatccggatg cgcagaatga cgccggacag caccacagac
      241 cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc
      301 tacccgaaca cggcactggt cggcgtacag gtggattcgg agcagttcgg cagccagcag
      361 gtgagccgta attatcatct tcgcgggcgc attctgcagg tgccgtcgaa ctataatccg
      421 cagacgcggc aatacagcgg tatctgggac ggaacgttaa aaccggcata cagcaacaac
      481 atggcctggt gtctgtggga tatgctgacc catccgcgct atggcatggg gaaacgtctt
      541 ggtgcggcag atgtggataa atgggcgctg tatgtcatcg gccagtactg tgaccagtcg
      601 gtgccggacg gctttggcag cacggagccg cgcatcacct gtaatgcctg gctgaccaca
      661 cagcgcaagg cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg tatgccggta
      721 tggaacgggc agacgctgac gttcgtgcag gaccgacagt cggataaggt gtgggcctat
      781 aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag tttcagcgcc
      841 ctgaaagacc gccataatgc cgttgaggtg aactggattg acccggataa cggctgggag
      901 acggcgacag agcttgtgga ggatacgcag gccattgtcc gttacggtcg taacgtcacg
      961 aagatggatg cctttggctg taccagccgg gggcaggcac accgcgccgg gctgtggctg
     1021 attaaaacgg aactgctgga gacgcagacc gtggatttca gcgtgggtgc tgaagggctt
     1081 cgtcatgtac cgggcgatgt cattgaaatc tgcgatgatg actatgccgg tatccgcacc
     1141 ggcgggcgcg tgctggcggt gaacagccag acccggacgc tgacgctcga ccgtgaaatc
     1201 acgctgccat cctccggcac cacgctgata agcctggttg acgggcaggg gaatccggtc
     1261 agcgtggagg tccagtccgt caccgacggc gtgaaggtaa aagtgagccg tgttcctgac
     1321 ggtgttgctg aatacagcgt gtgggggctg aagctgccga cgctgcacca gcgattgttc
     1381 cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc cgtgcagcat
     1441 gtgccggaaa aagaagccat cgtggataac ggggcgcact ttgacggcga ccagagcggc
     1501 acggtgaatg gtgtcacgcc gccagcggtg cagcacctga ctgccgaagt caccgcagac
     1561 agcggggaat atcaggtgct ggcgcgctgg gacacgccga aggtggtgaa gggcgtgagc
     1621 ttcctgctcc gtctgaccgt aacagcggat gacggcagtg agcggctggt cagcacggcc
     1681 cggacgacgg aaaccactta ccgcttcaca caactggcgc tggggaacta caggctgaca
     1741 gtccgggcag taaatgcgtg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt
     1801 gccgcaccgg cagcgctgtc gcggattgag ctgacgccgg gctattttca gataaccgcc
     1861 acgccgcatc ttgcggttta tgatccgacg gtacagtttg agttctggtt ctcggaaaag
     1921 cggattgcgg atatcaggca ggttgaaacc acagcacgct atcttggcac ggcgctgtac
     1981 tggatagccg ccagtatcaa tatcaaaccg ggccatgatt attactttta tatccgcagt
     2041 gtgaacaccg ttggcaaatc ggcattcgtg gaggctgttg gtcagccgag tgatgatgca
     2101 tccggttatc tggatttttt caaaggcgag atagggaaaa cccatctggc tcaggagctg
     2161 tggacgcaga ttgataacgg tcagcttgcg cctgacctgg ctgaaatcag gacgtccatt
     2221 acggatgtca gcaatgaaat cacgcagacc gtcaataaga aactggaaga ccagagtgcg
     2281 gcaattcagc agatacagaa ggttcaggtt gatacaaata ataacctgaa cagcatgtgg
     2341 gctgtgaagc tgcagcagat gcaggacgga cgcctttata tcgcgggtat tggtgccggt
     2401 attgagaaca cccctgacgg catgcagagt caggtgctgc tggcggcgga caggattgcg
     2461 atggttaatc ctgcgaatgg caacacaaaa ccgatgtttg ttggtcaggg cgatcagata
     2521 ttcatgaacg acgtgttcct gaaacgcctg acggccccca ccattaccag cggtggaaat
     2581 ccatcggcat tttccctgac tccggacgga aagctgaccg ctaaaaatgc ggatatcagt
     2641 ggcagtgtga atgcgaacgc cgggacgctc aacaatgtca cggtaaatga aaactgtacg
     2701 attaagggca tgctggaggc gactcaggtc agaggtgact tcgttaaagc tgtatccaaa
     2761 tcatttccga aacaggctgg tacgtggggt aacacggaaa caccaaacgg gacggttaca
     2821 gtcaccataa gcgatgatca taactttgac cgccagatta ttattccgcc cattattttt
     2881 aacggtatag cgtatgacga tccggggagc ggaaataacc caggaggcac gcgatacacg
     2941 ggttatggtt ttgaagttcg caaaaacggc gtattaatcg catccagaga aactaaaggt
     3001 gccattcccg gtagttacag tgcagttatt gatatgccta gtggtggtgg tagcgtcact
     3061 ctggagttta agattttcca gaaaggcaat cagggggcag gcaatatcac cgactgtacg
     3121 gtgattgtga ccaaaaaagc ggcttccggc atcagtattc gttgaaatat ttataacccc
     3181 aataaatggc gtcaggaatg acgccttttt tattgcagaa aagcgagagg taattatgcg
     3241 taaagtttgt gcagccattt tgtccgcagc catttgcctg gccgtatccg gtgcgcctgc
     3301 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgccc ggaccaacgt
     3361 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggatac
     3421 gctggggctg gtgacgtcat tcagctatgc aggagacaag aatcgccagc tgacccgtta
     3481 cagcgatacc cgctggcatg aagattccgt gcgtaaccgc tggttcagcg tgatggcggg
     3541 gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggtg tggcttacag
     3601 ccgtgtgtcg actttttccg gggattatat ccgcgtaact gacaataagg ggaaaacgca
     3661 cgatgtgctg accggaagtg atgacggtcg ccacagcaac acgtctctgg cgcactgacg
     3721 gtttcatcgt gggtgtcggt tataaattct gattagccag gtaacacagt gttatgacag
     3781 cccgccggtt caggcgggct tttttgtggg gtgaatatgg cagtaaagat ttcaggtgta
     3841 ctgaaagacg gcacaggaaa accggtacag aactgcacaa tccagctgaa agcaaaacgt
     3901 aacagcacca cggtggtggt gaacacgctg gcctcagaaa atccggatga agccgggcgt
     3961 tacagcatgg acgttgagta cggtcagtac agcgttattc tgttggtgga aggattcccg
     4021 ccgtcacatg ccgggaccat caccgtgtat gaagattccc gacccggtac gctgaatgat
     4081 tttctcggtg ccatgacgga ggatgatgcc cgtccggagg cactgcgccg ttttgagctg
     4141 atggtggaag aggtggcgcg taacgcgtcc gcggtggcac agaacacggc agccgcgaag
     4201 aaatcagcca gcgatgccag cacatcagcc cgtgaggcgg caacccatgc gactgatgct
     4261 gcagattcag cacgtgcagc cagcacgtca gccggacagg ccgcttcgtc ggctcagtca
     4321 gcgtcttcca gcgcaggaac ggcatcaaca aaggccactg aagcctcaaa aagtgctgcc
     4381 gctgcagagt cctcaaaaag cgcggcggct accagtgccg gtgcggcgaa aacgttagaa
     4441 acgaatgcgg cagcgtcaca acaatcagca gccacttctg catccaccgc gaccacgaaa
     4501 gcgtcagaag ctgccacctc agcccgggat gcgtcggctt caaaagaggc ggcaaaatca
     4561 tcagaaacga acgcatcatc aagcgccagt agcgcagctt cctcggcgac ggcggctgca
     4621 aattctgcga aggcggccaa aacgtccgag acgaacgcca ggtcttctga aacggcagcg
     4681 ggacagagtg cctcagctgc ggcaggctca aaaacagcgg ctgcgtcgtc tgccagcgcc
     4741 gcgtcaacaa gtgccgggca ggcctcagcc agtgccaccg ccgccggaaa atcggcagaa
     4801 agcgccgcat catccgcttc aacagccaca acgaaggctg gcgaagccac tgagcaggcc
     4861 acggcagcag cgaggtcagc ttccgcagcg aagacatccg aaacgaacgc gaaagcgtcg
     4921 gaaacccgtg cagaatcctc aaaaacggct gccgcatcgt ccgccagttc ggcggcgtca
     4981 tcggcatcat cggcgtctgc ttcaaaagat gaggcgacca gacaggcgtc agcagcgaag
     5041 ggcagcgcca cgacggcatc cacgaaggcg acagaggctg ctggtagtgc gacggcagca
     5101 gctcagagca aaagtacggc ggaatctgca gcaacgcgcg ctgagacagc ggcaaaacgg
     5161 gcagaggata ttgcatccgc cgtggcgctt gaggatgcga gcacgacgaa aaaggggata
     5221 gtacagctca gtagtgcgac taacagcact tccgagtcac tggcggcaac accaaaagcc
     5281 gttaaggccg cgtatgacct ggctaacggg aaatacaccg cacaggatgc aacgacagca
     5341 cagaaaggga taatccagct aagcagcgcg accaacagca cgtctgaaac gctggcggca
     5401 acgccaaagg cagtaaaagc agccaatgac aatgctgaga aacgtctgca gaaagatcag
     5461 aacggtgcgg atatccctgg caaagacacc tttacgaaaa atattggtgc ctgccgtgcc
     5521 ttcggtgggt cagtaagcac aacaacagga aactggacga ctgcacagtt tatcgagtgg
     5581 ctggattctc agggagcatt taaccatcca tactggatgt gcaagggttc ctggtcttat
     5641 ggcaataata aaatcattac tgatactggc tgcggtaata ttcatctcgc cggagctgtc
     5701 attgaagtaa tggggataaa gtcagcgatg acgatccgca ttaccactcc gaccacatcc
     5761 agcggtggcg gaacaaccaa cgcccagttt acctatatta atcacggaac agattattca
     5821 cctggctggc gaagggacta taactccaga aataagccaa cggcatcaga gatcggggcg
     5881 ctaccgtcag gtggaacagc agtatcatca gttaatctgg cttcaaaagg tcgggtagcc
     5941 gcgctgacag acaatacgca gggggcagca ggtcttgagt tatacgaggt gtataacaac
     6001 ggatatccaa cagcgtatgg aaatatcatt cacctgaaag ggatgacagc cgttggcgaa
     6061 ggtgagttac tcatcggctg gagtggtaca agcggtgctc atgctccggc atttattcgt
     6121 tcacgacggg atacgaccga cgcaaactgg tcgccgtggg cgcagcttta cacctcggct
     6181 catcctcctg cagagtttta tccagtcggt gcaccaatcc cgtggccatc agataccgtt
     6241 ccgtctggtt atgccctgat gcaggggcag acttttgaca aatctgctta cccgaaactt
     6301 gcagccgctt atccgtcagg cgtgatccct gatatgcgtg gctggacgat taagggcaag
     6361 cccgccagtg gtcgtgccgt attgtctcag gaacaggatg gcattaaatc gcacacccac
     6421 agcgccagcg catccagtac ggatttgggg acgaaaacca catcgtcgtt tgattacggc
     6481 actaaatcca cgaataacac cggggcgcat acgcacagtc tgagcggctc tacaagttca
     6541 gctggggccc accaacatag tcagactggg cccagaacga atagcgggag tcaacctact
     6601 ggaatgtttc cggcagggag tactcaagtt agcgggacta atcaggttgg tatcagtggc
     6661 tcattaacca gtggcacctc ccaatgggta ggtaaatcat cttcagaagg aaaccataca
     6721 cactcactgt ccggcacagc agcatctgcg ggtgcacacg cgcatactgt cggtattggt
     6781 gctcatacgc actctgttgc gattggttca catggacaca ccatcaccgt taacgctgct
     6841 ggtaacgcgg aaaacaccgt caaaaacatc gcatttaact atattgtgag gctcgcataa
     6901 tggcattcag aatgagtgaa caagcacgga ccataaaaat ttataacctg ctggccggaa
     6961 ctaatgaatt tattggtgaa ggtgacgcat atattccgcc tcatacaggt ctgcctgcaa
     7021 acagtaccga tattgcaccg ccagatattc cggctggttt cgtggctgtt ttcaacagtg
     7081 atgagtcatc gtggcatctc gttgaagatc atcggggtaa aacggtttat gacgtggctt
     7141 ccggcgacgc gttatttatt tctgaactcg gctcattacc ggaaaatgtc acctggttat
     7201 ccccggaagg ggagtttcag aagtggaacg gcacagcctg ggtgaaggat acggaagcag
     7261 aaaaactgtt ccggatccgg gaggcggaag aaacaaaaaa cagcctgatg caggtagcca
     7321 gtgagcatat agcgccgctt caggatgctg tggatctgga gatcgcaacg gagaaagaaa
     7381 ccttgttgct ggaagcttgg aaaaagtatc gggtgttgct gaaccgtgtt gatacatcaa
     7441 ctgcaccgga tattgaatgg ccagtagcac ctatagggta aattcgtaat gattacctaa
     7501 atacgttatt cttttgttaa aaaagtgatt ttgctatagg tagggagtat gttcttggat
     7561 taagaataaa aaatcgccat gaaagagctt catggcgagt acataaacgt acaaaatgtg
     7621 atgaaagtag agtggcgtaa gccactatat taagatatga caatagcgaa gaaaagtaaa
     7681 taacatttta tggacgttta attgttaatt ataaaaacta gagtgcccga tattgtttat
     7741 cttgctttaa aataatgtac tcaaaccaaa tttttgagct aaaagctcaa ataatataaa
     7801 tgcaataata atgtttaata agacaattag cttgctttgt attttcattt cggaatactg
     7861 ttaagtaact ttgatgaccc taacaataga tc
//
LOCUS       DAJVXP010000166         7891 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVXP010000166 DAJVXP010000000
VERSION     DAJVXP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.166"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..183)
                     /locus_tag="OQS10_003644"
     CDS             complement(<1..183)
                     /locus_tag="OQS10_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS2294572.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQAT"
     gene            complement(300..1346)
                     /gene="ansB"
                     /locus_tag="OQS10_003645"
     CDS             complement(300..1346)
                     /gene="ansB"
                     /locus_tag="OQS10_003645"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS2294573.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1522..2241)
                     /locus_tag="OQS10_003646"
     CDS             complement(1522..2241)
                     /locus_tag="OQS10_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS2294574.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2425..2751)
                     /locus_tag="OQS10_003647"
     CDS             complement(2425..2751)
                     /locus_tag="OQS10_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS2294575.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(2751..3470)
                     /gene="trmB"
                     /locus_tag="OQS10_003648"
     CDS             complement(2751..3470)
                     /gene="trmB"
                     /locus_tag="OQS10_003648"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS2294576.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3631..4683
                     /gene="mutY"
                     /locus_tag="OQS10_003649"
     CDS             3631..4683
                     /gene="mutY"
                     /locus_tag="OQS10_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS2294577.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4711..4986
                     /locus_tag="OQS10_003650"
     CDS             4711..4986
                     /locus_tag="OQS10_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS2294578.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5051..6130
                     /gene="mltC"
                     /locus_tag="OQS10_003651"
     CDS             5051..6130
                     /gene="mltC"
                     /locus_tag="OQS10_003651"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS2294579.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6332..7588
                     /gene="nupG"
                     /locus_tag="OQS10_003652"
     CDS             6332..7588
                     /gene="nupG"
                     /locus_tag="OQS10_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS2294580.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7637..>7891)
                     /locus_tag="OQS10_003653"
     CDS             complement(7637..>7891)
                     /locus_tag="OQS10_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS2294581.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1859 a         2086 c         1930 g         2016 t
ORIGIN      
        1 ggtggcctga ttacccttag catcaagatt ttttaaggtg atagcatcaa gcttgagaat
       61 gccgtgatac tgctttttga attgatcttt taagatattt tcagttggcg gttcgccatc
      121 tgcccaggca ttaccagtca tcaacataag cagtgccgtg ccaacaatcc attgcttttt
      181 cacccgaaaa acctcacgcg gaataattta ttccggtgat tatcattagg gtaatgaaaa
      241 ttggcaatgg acgtaagtct gaagtgaaaa agccccggca cgataccggg gcgaggtgat
      301 tagtactgat tgaagatctg ctggatctgc tgcggatctt tggtttgcgt cagagccagc
      361 tgcagcagga cgcgcgcttt ttgcgggttc agcgtgccag aggcgacgaa gccgtatttc
      421 gcatcatcca cttcagcatc ctgagtggta gcacccgtcg gtacgcggga agaacgcact
      481 actgcagtgc cgtttttcgc ggcggttgcc agggtgtcga acacggattt atacaggtta
      541 ccattaccca caccagcgct aacgatgcca tcatagcccg catctaccag tgctttagcc
      601 ggaagatcgg atgcgttagc gtagttataa acgatgccga ctttcggcag ctcattcagc
      661 ttagagacat cgaatggcgt atcgctggtg tgcttacgtg ccggggtacg ttggtagtca
      721 atcttaccgt tgtgaatgta tcccagagga ccgtagttaa cagacttgaa ggtcgctacg
      781 tcggtggtgt tggttttggt gacatcgcga ccgtccagta cggtgtcgtt catcaccacc
      841 agcacgccac gattagcgga ggctttatca gctgcggtca ctaccgcgtt atacaggttg
      901 aatggaccgt ctgcgctcat ggacgtggac gggcgcattg cgccgaccat caccaccggt
      961 ttgtcgcatt tcaccgtcag gtcgaggaag taagcggttt cttccatcgt gtcggtaccg
     1021 tgggtaatga cgaagccgtc ggttttatcg cagtcggtgt taattttttt cgccagtgtc
     1081 agccagacat tatcgttcat gtcctgggag ccgatattca ctacctgctc gcctttaacg
     1141 ttcgcaatgt cttttagttg cggcaccgca ttaaccagat tttctacgcc aactttaccc
     1201 gctgtgtagt tagatttggt tgcggagtca ccaccaccgg caatggtccc gccggttgct
     1261 aaaatggtga tattgggtaa tgccaatgct gcaccactaa aacccataac cagtgcggca
     1321 agtgccgtct ttttgaaaaa ctccatttca ttcctccagt tacgtgaacg ctacgcatta
     1381 tcccttagct ctgtatggga aatttgacgt taaacaattt acaacgtgaa tatattttgg
     1441 agatctacaa agttagaggc aggtaacaaa acgaagaatt aaacggcata aaaaagtatt
     1501 atgccgtctt aaaatagagg attattttaa attaccgacc agggctttgc ggctatcttc
     1561 cagagtcaca acgcggctac aaacatcttt gccaaactgc tggaaatctt tttcctgctt
     1621 tttccactct gtttggattg aggattgcag cccccccagg cttcccagca cattctgtaa
     1681 tgggttaccg ccgcttttca gcaccgcttt cgcgcccatt tcattaatgc tgtcctgtaa
     1741 aattccaccc attgcctgat tcactaattg ctggccttcg gcgcgaacct gatcaatggc
     1801 tttatagtga aacgtcaggc catcgctgcg cgtttcgata atgcggttca tctgctcttt
     1861 cagctgcgca tcaagtttgg tcagacggct gcgcattttg ctgctttcgc ccatctcctg
     1921 aacgataatt ttatccagcg caatacgggc tttttcgacg cggcttttcg cgccttcatc
     1981 aatccacggc agcgtgctgc gtagttcagc ctgataatcc ttcgcctgct cgcgctgggc
     2041 ggcattcagg gaatattgct taccgttata catcacgttg ccgtctggcg tgatcaccag
     2101 attgccgttt tcgcccttca cctgcacggt ttgcgggctg acaatcacat cgtcacgcgg
     2161 cgtgacgctg cactggtagt cggcgtgagc ggtcattgcc gtcactgaaa gtgctgccgc
     2221 cagcagcatt ttgcgcatca tagtcttccc tcaagaaaaa atcaggccag catttgctgg
     2281 ccccagattg ataacaaagt acgcgttttc catgccggat gcggcgtaaa caccttatcc
     2341 cgcctactcg atcgtgcatc tttccaggcc tgataagcgt agcgcatcag gcagttttgc
     2401 atttgtcatc gcccgtatgc tttcttagtc ccaccaaacg tcgaaaagtt cgctggtgcg
     2461 tacctcatcc agtttgcgct cttccagcca cttacgcaca atcgcctgat gttcttcggt
     2521 gcatttgccg atttcctgca tgcagatcag accttcccag gccagataac cgctgccgtc
     2581 aaaggccagt ttgttcggtt cgataacctc gttaataaaa tcatcaacgg ttttatcaat
     2641 ctgttcttcc gatgtacctt ccgggaatcg ccatgccacc gaaaatccta attcctggaa
     2701 ttcgtcgatg tgcatttttt tacgcagacg acggctacgg ttctttgcca ttatttcacc
     2761 ctctcgaaca ttaagtccca tactccgtga ccaagacgat gaccacgttg ttcaaatttc
     2821 gtcaccggac gtgatgccgg acgcggcacg taatcattgc tctctgacag gtttttataa
     2881 ccgtcaatag aagacatcac ttcaagcata tgttccgcat aaggttccca gtcggtcgcc
     2941 atatggaata caccccccag ctgcagtttg ctttttacca gttcggcaaa cggcacctgt
     3001 acgatacggc gtttattatg gcgcgctttg tgccacgggt cagggaaaaa gagctgcacc
     3061 atgcgcaatg aattgtcagg aatcatttta tgcagcactt caaccgcatc gtgacacatc
     3121 acgcgcaggt tgcttaaacc ttcttcatgc gcagaagcca ggcacgcacc aacgcccggt
     3181 gaatgcactt caatgccgag gaagtcctgc tcagggcgat ctttagccat tgccaccagc
     3241 gacgccccca tgccaaaacc aatctcaagc gtcaccggcg cttcacggcc aaaaagcgcg
     3301 gggaaatcca gcatatcttc gctgaactca acgcccatca ccggccagta gttttccagc
     3361 gcatgttcct ggcctttggt cagtcgcccc tggcggcgca caaaactacg gatacggcgc
     3421 agtgggcggc cgttttcatc aaattccggt gaaatgacgt cgtttttcat aaagatttag
     3481 tcgcttgtga aagtgttctg aaaacgggca ttatccaaag ttagttgccg gatgcaagca
     3541 tgataaggcc gtggctgcgg aaagttccgg tttacaccct gccgtcgctg tgctgcaatc
     3601 ttgcccccaa caacagtgaa ttcggtgacc atgcaagcgt cgcaattttc agcccaggtt
     3661 ctggactggt acgataaata cgggcgaaaa acgctgccct ggcaaattga caagacgccc
     3721 tacaaagtat ggctctcaga agtgatgttg caacaaactc aggttgcgac cgttatcccc
     3781 tattttgaac gctttatggc gcgcttcccg acggtgaccg atctcgccaa tgcgccgctc
     3841 gacgaagttc tccacttgtg gaccgggctt ggctattacg cccgcgcgcg caatctgcat
     3901 aaagcggcac aacaagtggc gaccttacac ggcggtaaat tcccggaaac ctttgaagaa
     3961 gtcgcggcgt taccgggcgt cgggcgttcc accgcaggcg cgattctctc gctttctctg
     4021 ggtaagcact ttccgattct cgacggtaac gtcaaacgcg tgctggcgcg ctgctatgct
     4081 gtaagcggct ggcctgggaa aaaagaggtc gagaataaat tatggagttt gagcgagcag
     4141 gtgacgcccg cggtcggcgt ggaacggttt aatcaggcga tgatggattt gggcgcgatg
     4201 atttgtacgc gctcgaagcc gaaatgttcg ctctgtccgc tacaaaacgg atgtattgcc
     4261 gccgccaata atagctggtc gctttatccg ggcaaaaaaa cgaaacagac gctgccggag
     4321 cgcaccggct actttttgct gttacagcac gaagatgaag tattgctggc gcagcgtccg
     4381 ccgagcggat tgtggggcgg tttatactgt ttcccgcagt ttgccgacga agaaagtttg
     4441 cggcagtggc tggcgcaacg gcagattgct gccgataacc tgacgcaact gaccgcgttt
     4501 cggcatacct tcagccattt ccacttagat attgtgccta tgtggcttcc cgtgtcgtca
     4561 ttcaccggct gcatggatga aggcaatgcg ctctggtata acttagcgca accgccgtca
     4621 gttggcctgg cggctcccgt ggagcgtttg ttacagcagt tacgcactgg cgcgccggtt
     4681 tagcgcgtga gtcgataaag aggatgattt atgagcagaa cgattttttg tactttcctg
     4741 caacgtgaag cagaaggtca ggattttcag ctgtaccccg gcgagctggg aaaacgcatc
     4801 tataacgaga tctccaaaga agcctgggcg cagtggcagc acaagcaaac catgctgatt
     4861 aatgaaaaga aactcaacat gatgaatgcc gagcaccgca agctgcttga gcaggagatg
     4921 gtcaacttcc tgttcgaggg taaagaggtg catatcgagg gctatacgcc ggaagataaa
     4981 aaataaaaac agtgccggag cacgcctccg gcaacttgca taaaaacaaa cacaacacgc
     5041 acccggaatg atgaaaaaat atctcgcgct ggctttgatt gcgccgttgc tcatctcctg
     5101 ttcgacgacc aaaaaaggcg atacctataa cgaagcctgg gtcaaagata ccaacggttt
     5161 tgatattctg atggggcaat ttgcccacaa tattgagaac atctggggat tcaaagaggt
     5221 ggtgatcgcc ggtcctaagg actacgtgaa atacaccgat caatatcaga cccgcagcca
     5281 catcaacttc gatgacggta cgattactat cgaaactatc gccgggacag aacctgccgc
     5341 gcatttgcgc cgggcaatta tcaaaacgtt gctgatgggt gacgatccga gttcggtcga
     5401 tctctattcc gacgttgatg atattacgat ttcgaaagaa cctttccttt acggtcaggt
     5461 ggtggacaac accgggcagc cgattcgctg ggaaggtcgc gcaagcaact tcgcggatta
     5521 tctgctgaaa aaccgtctgc aaagccgcag taacgggctg cgtatcatct acagcgtcac
     5581 cattaacatg gtaccgaacc accttgataa acgtgcgcac aaatatctcg gcatggtccg
     5641 ccaggcgtca cggaaatatg gcgttgatga gtcgctgatt ctggcaatta tgcagaccga
     5701 atcttccttt aacccgtatg cggtcagccg ttccgatgcg ctgggattaa tgcaggtggt
     5761 acaacatact gccgggaaag atgtgttccg ctcgcagggg aaatccggca cgccgagccg
     5821 cagtttcttg tttgatcctg ccagcaatat tgataccggc accgcgtatc tggcgatgct
     5881 gaacaatgtt tatctcgacg gaattgataa cccaacatcg cggcgttatg ccgtcatcac
     5941 cgcctataac ggcggcgcag gcagcgtgct gcgagtcttt tcgaatgata agattcaggc
     6001 tgccaatatt attaacacca tgacgccggg cgatgtttat cagacgctga cgacccgcca
     6061 tccctctgcg gaatctcgcc gttatcttta taaagtgaat accgcgcaaa aatcctaccg
     6121 ccgccgataa ttccattaac cgcccctgac gatgctcagg ggcaaaaatg ttatccacat
     6181 cacaatttcg ttttgcaaat tgggaatgtt tgcaattatt tgccacaggt aacaaaaaac
     6241 cagtccgcga agttgataga atcccatcat ctcgcacggt caaatgtgct ttttcaaaca
     6301 ctcatccgca tcacaatgtg aggaaattaa catgaatctt aagctgcagc tgaaaatcct
     6361 ctcttttctg cagttctgtc tgtggggaag ttggctgacg accctcggct cctatatgtt
     6421 tgttaccctg aagtttgacg gtgcttctat tggcgcagtt tatagctcac tgggtatcgc
     6481 cgcggtattt atgcctgcgc tgctggggat tgtggccgac aaatggttaa gtgcgaaatg
     6541 ggtatatgcc atttgccaca ccattggcgc tatcacgctg ttcatggcgg cacaggtcac
     6601 gacgccggaa gcgatgttcc ttgtgatatt gattaactcg tttgcttata tgccaacgct
     6661 tgggttaatc aacaccatct cttactatcg cctgcaaaat gccgggatgg atatcgttac
     6721 tgacttcccg ccaatccgta tctggggcac catcggcttt atcatggcaa tgtgggtggt
     6781 gagcctgtct ggcttcgaat taagccacat gcagctgtat attggcgcag cactttccgc
     6841 cattctggtt ctgtttaccc tgactctgcc gcatattccg gttgctaaac agcaagcgaa
     6901 tcagagctgg acaaccctgc tgggcctcga tgcattcgcg ctgtttaaaa acaagcgtat
     6961 ggcaatcttc ttcatcttct caatgctgct gggcgcggaa ctgcagatta ccaacatgtt
     7021 cggtaatacc ttcctgcaca gcttcgacaa agatccgatg tttgccagca gcttcatcgt
     7081 gcagcatgcg tcaatcatca tgtcgatttc gcagatctct gaaaccctgt tcattctgac
     7141 catcccgttc ttcttaagcc gctacggtat taagaacgta atgatgatca gtattgtggc
     7201 gtggatcctg cgttttgcgc tgtttgctta cggcgacccg actccgttcg gtactgtact
     7261 gctggtactg tcgatgatcg tttacggttg cgcattcgac ttcttcaaca tctctggttc
     7321 ggtgtttgtc gaaaaagaag ttagcccggc aattcgcgcc agtgcacaag ggatgttcct
     7381 gatgatgact aacggcttcg gctgtatcct cggcggcatc gtgagcggta aagttgttga
     7441 gatgtacacc caaaacggca ttaccgactg gcagaccgta tggttgattt tcgctggtta
     7501 ttccgtggtt ctggccttcg cgttcatggc gatgttcaaa tataaacacg ttcgtgtccc
     7561 gacaggcaca cagacggtta gccactaatt acgcaaagaa aaacgggtcg ccagaaggtg
     7621 acccgttttt ttattcttac ttcaacacat aaccgtacaa tcgtttcatg ccatccgcat
     7681 cggtttcgct atagacacct tgcaactccg gcgaaaatcc cggcaacaaa ttcacgcctt
     7741 cttccagcgc aaggaaataa cgttgaaccg ccccacccca gacttccccg ggaaccacgc
     7801 aaagcacgcc aggtggataa ggcaacgccc cttctgccgc aattcgccct tcggcatcac
     7861 gaatccgcac caactccact tcaccgcgaa t
//
LOCUS       DAJVXP010000167         7814 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVXP010000167 DAJVXP010000000
VERSION     DAJVXP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.167"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..310
                     /locus_tag="OQS10_003654"
     CDS             <1..310
                     /locus_tag="OQS10_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS2294582.1"
                     /translation="KSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIASVRSNSPQ
                     EERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGTSQTVKDNT
                     "
     gene            457..1818
                     /gene="yegQ"
                     /locus_tag="OQS10_003655"
     CDS             457..1818
                     /gene="yegQ"
                     /locus_tag="OQS10_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS2294583.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2148..2465)
                     /locus_tag="OQS10_003656"
     CDS             complement(2148..2465)
                     /locus_tag="OQS10_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294584.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            2871..3770
                     /gene="yegS"
                     /locus_tag="OQS10_003657"
     CDS             2871..3770
                     /gene="yegS"
                     /locus_tag="OQS10_003657"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS2294585.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3852..4625)
                     /locus_tag="OQS10_003658"
     CDS             complement(3852..4625)
                     /locus_tag="OQS10_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2294586.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4731..5771)
                     /gene="gatD"
                     /locus_tag="OQS10_003659"
     CDS             complement(4731..5771)
                     /gene="gatD"
                     /locus_tag="OQS10_003659"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS2294587.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5819..7174)
                     /locus_tag="OQS10_003660"
     CDS             complement(5819..7174)
                     /locus_tag="OQS10_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS2294588.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7178..7462)
                     /gene="gatB"
                     /locus_tag="OQS10_003661"
     CDS             complement(7178..7462)
                     /gene="gatB"
                     /locus_tag="OQS10_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS2294589.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7493..>7814)
                     /gene="gatA"
                     /locus_tag="OQS10_003662"
     CDS             complement(7493..>7814)
                     /gene="gatA"
                     /locus_tag="OQS10_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS2294590.1"
                     /translation="AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQAD
                     DDNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVL
                     DSDE"
BASE COUNT         2113 a         2007 c         1757 g         1937 t
ORIGIN      
        1 taagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg agactatcct
       61 caccagcgag ctgtatacat caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag
      121 caacagcccg caagaagaac gctatgagaa aaaaacggca agtaacggca aattctattt
      181 caatctgaaa gccgccaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca
      241 atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga
      301 caatacgtaa ttacgatgcc gggcgtgggg tgtcggcgtc cggcgcttta ccagatcccc
      361 ccacagcgct acaatgcccg cccttaaagt gggggcactc ccctaaccgc ttcatcaggt
      421 gaagcggagc tgacctgtca tcagaacgag agaattatgt ttaaaccgga actcctttcc
      481 ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt atggcgcaga tgctgtttat
      541 gcgggccagc cgcgttactc cctgcgtgtg cgcaacaacg aattcaacca cgaaaatctt
      601 cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt tttatgtcgt ggtcaacatt
      661 gcaccgcaca acgccaagct gaaaaccttt atccgtgacc tgaaaccggt ggtggaaatg
      721 gggccggatg cgctgattat gtccgatcca gggctgatta tgctggtgcg tgagcacttc
      781 cctgaaatgc cgatccacct ctcggtgcag gctaacgccg taaactgggc gacggtgaaa
      841 ttctggcagc aaatgggcct gacccgcgtg atcctctctc gcgagctgtc gctggaagag
      901 attgaagaga tccgcaatca ggtgccggat atggagattg agatcttcgt tcacggtgcg
      961 ctgtgcatgg cctactccgg tcgctgcctg ctctctggct atatcaacaa gcgcgatccg
     1021 aaccagggta cctgcaccaa cgcctgccgc tgggagtaca acgtccagga agggaaagaa
     1081 gatgacgttg gcaacatcgt acacaagtac gagccgattc cggtgcaaaa tgttgagccg
     1141 acgctaggta tcggcgcgcc aaccgacaaa gtgtttatga tcgaagaagc ccagcgtccg
     1201 ggcgagtata tgaccgcgtt tgaagatgag cacggcactt acatcatgaa ctcgaaagat
     1261 ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg gcgtgcattc gctgaaaatc
     1321 gaaggccgta ctaaatcttt ctactattgc gcacgcaccg cacaggttta tcgtaaagct
     1381 atcgatgatg ccgctgcggg caaaccgttc gataccagcc tgctggaaac tctggaaggt
     1441 ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc atactcacga cgattatcag
     1501 aactacgaat acggttattc agtttctgac cgccagcagt ttgttggtga gtttaccggt
     1561 gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata aattctccgt tggcgacagc
     1621 cttgagctga tgacgccgca aggcaacatt aactttaccc ttgagcacat ggaaaacgcc
     1681 aaaggtgaag cgatgccggt cgcaccaggc gatggttata ctgtgtggct cccggtcccg
     1741 caggatcttg agctaaatta cgcgctgctg atgcgtaatt tctccgggga aaccacgcgt
     1801 aacccacacg gtaagtgatt aatttcgatt atttttcccg gatggaaaat tcttagaaac
     1861 cgatcacata cagctgcatt tattaaggtt atcatccgtt tcgctgaaaa acataaccca
     1921 taaaatgcta gctgtaccag gaaccacctc cttagcctgt gtaatctccc ttacacgggc
     1981 ttatttttta tgcataagcc ctatccctgg tcaccgtctt ccattgacca catcgataga
     2041 atctcccttc atagcacgat gcctttcacg taacggcatc gtgctcgcac aggttccggc
     2101 taagcacaac cagaacgcgc atgtttgatg cttaccaaaa aatattctca ctctccacat
     2161 ttgaatgtca gacgagcgac gccatgtaat cctgcacctt ctgtcttcag gtcaactatc
     2221 tgcatttttt tgccctgagt aacacagaaa tgggctgcat cattttttac tatattttct
     2281 gcaccagata ttcttcccct agctaaagaa gcttcggctt cggtgtagta ttggttatcg
     2341 agtttacgct gaatattact tttatatgca agaccaaatt taccgatact tgtttcatca
     2401 ttatgcacag cacaaccaga tataagaaaa atactaatta atgatatagc agctatcttt
     2461 ttcatctcac cttcccccat taaataccaa cgacactctc tagtgtttaa atataataat
     2521 ggcatgatta ttataattga ataggattat aataaatgtt ctgtacaaca tttcctacat
     2581 aagtaggaat tacggacatt gaggcccttc agggtaactc catgggggct ttaatatatt
     2641 atattgaaga tgccactgtt tagttgaata ttaggtatat gctctttttt gaaatttatc
     2701 ggtggcagcc gttagtattc gctgtcccca ttgcaagctc ctggtggtaa ccactgaatc
     2761 ctccatactt gaactgactt tttatcctcc gactttcatc ctgttctgac tccacctttt
     2821 gttttctgct ctacactatc tacagaccaa tcataaaggc acatacgatc atggcagaat
     2881 ttcccgccag cttactgatt cttaatggca aaagtactga caatctaccc ttgcgcgaag
     2941 caattatgct gttgcgtgag gaaggaatga cgatccatgt gcgggtcacc tgggagaaag
     3001 gcgatgccgc acgatatgta gaggaggccc ggaagttggg cgtcgcaacg gtgattgccg
     3061 gtggtggcga tggcaccatt aatgaagttt ctacggcgtt gattcagtgt gagggggatg
     3121 gcatacccgc gctgggaatt ttgccattag gaaccgccaa tgattttgcc accagtgtag
     3181 ggattcctga ggcactggat aaggcgctga aactggcaat tgccggtaac gccattgcga
     3241 tagatatggc gcaggtcaac aaacaaacct gttttattaa tatggcgaca ggcggatttg
     3301 ggacgcgtat taccacagaa acgccggaaa aattaaaagc cgcgctgggt ggcgtctctt
     3361 acatcattca tggcttaatg cgcatggata ctctgcaacc ggaccgttgt gaaatccgcg
     3421 gtgaaaactt tcactggcaa ggtgacgccc tggtcattgg tattggtaac gggcgtcagg
     3481 ccggtggcgg tcagcaattg tgtccgaacg cgttaattaa cgatggcttg ctgcaactgc
     3541 gcatttttac cggcgatgaa attcttccgg ctctcgtatc aaccttaaaa tctgacgaag
     3601 ataacccgaa tattatcgaa ggcgcttcgt cgtggtttga tatacaagcc ccacacgaaa
     3661 tcacttttaa tcttgatggc gaaccgttga gcggacaaaa ctttcatatt gaaatacttc
     3721 cggcggcgtt gcgttgtcga ttaccaccag attgtctatt attgcgttaa tcaaactatt
     3781 ctctcttgaa ccagggagaa tccgaaatgc cggatgcggt gtaaatacct tatccggcat
     3841 acagaacaat actacaccgt aattaatttt acccgtgtct tctgcaatgc cagtttatcc
     3901 acctctgata cagcatcatc ggtaataata atgtcaattt tttcgattgg cagtacctga
     3961 ttaaaaccgc gacggttaaa tttcgatgaa tcgagaacgg caatcacttt attagcggcg
     4021 gtgaccatcg caccgctaat cgaataacct tcattaaagg tcgtaatacc attaaccgca
     4081 tcaataccat cagcaccgac aaacattaaa tcggcattaa tatcctgcaa tgaacgctcg
     4141 gcaatggaac catgcatcga gcgcgtttta tggcggaccg tgccaccaca gacgaccaga
     4201 gtaatgtctt tattttcgga aagggcaaac gctgccggga gactgttggt aatcaccgtg
     4261 atatttttag cggtcattaa tccttcggcg ataagcattg tcgtacttcc gctgtcgaga
     4321 ataacggtca tcccttcatg gatcattttg actgccgcct gcgctattct gttttttggc
     4381 gcgctggcga gctgaaatcg ctctttaaac ccgacttcct gggtctcggt ttcactatta
     4441 ccggacatta ttttcgccgc accgccatga aagcgcgtaa ccacgccttt ttgttcgaga
     4501 aagcgcaaat cggcacggat tgtcgcttcc gaggcagcaa atactcccgc cagatcctga
     4561 acaagcacgg ttccctgttc attcactaat tggatgatct tattccttcg ctcgaatgaa
     4621 ttcatcgtca tttacccata ttcaattgtg gctagtgtaa acgaaggggg accattggtg
     4681 aataatctgt tttgcacaat accgggccag catcacgctg gcccgcggtt tcatggaatg
     4741 agcaacactt tgcccggcat ggcattacga gcgatgtcac gcaccgcctg ggtgaagctt
     4801 tcaaagcttc cacggtgagc gattaatggc tccaggctta acttacgttc tgtcagcaac
     4861 cggctcgccg tttcccactc ctgccccggc caagggctgg aatagttcat ccaactgccg
     4921 ataaccgtca gctctttacg caatattttg ccaaacgttg ccgatgttaa atgcagatcc
     4981 tgatgcaacg tgcccaccag cgccagttgg gcatgaggac cggcaatctc taccgccagt
     5041 tcgacagttt gcggtacgcc agccgtctcg aggataagct gattaaagcg cagttcgcgt
     5101 aaaacgctct gcatttgcgg cgcgctcatt tcgctactgt taaatgtttg catcgcaccg
     5161 aaagattttg ccaatgccag tttttctgag ctaatgtcga tcgccgtcac actctttgct
     5221 cccagcgcga cagcgcactg aatagccagc aggccaatgg ttccggcacc aataataata
     5281 acgtttttat tctcacaacc ttgcgctaaa tgaaaagcat gcaggccaac ggtaatcggc
     5341 tcaataaaag ccccatcctc aataggcata tccgtgggta gagcaaagac attttttcgc
     5401 ttaacgacaa tatattcagc aaatccacca tcacgccgcg agccaataaa atcatatttt
     5461 gcgcactggg aataaaaccc tttcaaacac tctggataag taaaacaggg taataacggc
     5521 acacaggcaa ccgcatcgcc agggtgtaaa tcatcaacac cggatcccac cgcatcaata
     5581 tagccgctaa attcatggcc taacgttatt ggataataat gtgcaccatt tttaaatatc
     5641 ctgggtaaat cggaaccaca taagcccgag ctggcaattt ttacccgcac ctcatcctga
     5701 tgtttaattt caggaatgac gctttctgca acgcgcacga taccatcagt atcattcacc
     5761 attgatttca taaaaactcc tgattgttaa gggggataac ctccccctca ggtaaaaatt
     5821 attctgcgag aacggttttc tcttgtttaa taaaaccacg cgctctacgc caggtcatga
     5881 aaataccggt aagataaatt gcgccgataa taatgaaacc gggaatattt tgcgtggaga
     5941 aaacctgaat cagtaaccag gtaatgggag aaccgccctg atccattgaa gccaccatac
     6001 ccccggcttt taacgcccca gcattagccg ccagttgggt gtgcaaacca atagtttgcg
     6061 tcgcgatcca cagggtgatg ctcataatga tgacacccga gattaaggtg cggaacagat
     6121 ttccacgatg caccgcgacc gccatcgcca cgaagaagcc gatggtggca agatcgccaa
     6181 acggcagtac ctgattaccc ggaacacaaa cagcaattaa aatggtgagc gggataaaaa
     6241 tcaggcttgc cgataccacc gccgtatgcc ccagcagtaa tgcgggatcc aggccaatca
     6301 ggaactcctg accgccgaac ttcgcctgta aacgactacg ggcctgctta gcgatgggcg
     6361 ttaaaccatc catgatgggt ttaatcaccc gtggcatcag cagcataact gccgccgttt
     6421 ttaccgccag ctgcaataca cctttgacat cgtaaccggc gaggatgccg ataatcagcc
     6481 ccatcacaaa acccacggtg acaggctcgc caaatggacc aaatttgcgc tgaatatcgt
     6541 cggcgctaaa tttaattcgg ttaacgcctg ggattttctc gatgatagca tcgaccagca
     6601 ccgcaatcgg ccccatatac gccgacgtac cgtgcggaat agcaatacct tccagctcaa
     6661 agaaatttcg ggtatcgcgg gcaaaccagt cgccgagctt ataaacaaac gccgcgtgaa
     6721 ttaccacacc tgccatccct atcatccatg aaccggttgc cagatgcagc aacgcgccgg
     6781 tgaaggtcat atgccagata ttccagatat caacatttac cacccgcgtc atacgggtca
     6841 gtaacatcgc cacgttaacc agaatcgcaa tcggaatcgc caccagcgca atttgcgacg
     6901 cccaggtcat tggtgaagag cccggccagc cgacatcgac cacatgcaga ttcaggtcga
     6961 aattttccgc catcgctttc gccgccggac caatggaatc cagcattaag ccaatcacaa
     7021 ggccaatgcc aacaaagcca atcccgatat gcagacccgc tttaaagcaa tcgcctgcct
     7081 tcatgcctaa tattttagaa aaaataatga tgacaatcgg aagcatcacc gtagggccga
     7141 ggtcgagaat ataacgcatg acttctgaaa acataggtca cccctgtaag atagtcagaa
     7201 ttttattttg taatgcttcg ataccgacac cagaaataaa aggcatgccg tgaactaacg
     7261 gaatatcgcc aaaactacga tccactttgg cagtggtgca tatcaaatgc acaccatcca
     7321 tataggtttc tatttcatta acccgacact ggattaattc aacaggaata ctatgattct
     7381 gacacaactc tttaatttct tccgccgcca tcgtagaggt cgcaaccgcg cctccgcaag
     7441 cgacaataat cttgcgtttc ataaatagtc ctttttattg ttacagaggg atttattcgt
     7501 ctgaatctaa aacatacttt gtgaagtatt cctttaactg ggtttcagga agagtgatta
     7561 gtgtctcgac gatatcgggc tgttgtaact tgccaaataa acagcgtaaa agtttcaatt
     7621 gctgctgcgg attctccaca atcaacgcaa taaccaacga tacctccacg tcgttatcat
     7681 catccgcttg ctgaaaatga actttatttg ttggccttaa cagataaatg gctgacgact
     7741 tagcatgaat cgcctcacaa tgcggtattg caatggcgtg ctgctcaagc attatcccgg
     7801 tagggaattc tgct
//
LOCUS       DAJVXP010000168         7776 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVXP010000168 DAJVXP010000000
VERSION     DAJVXP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.168"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1780)
                     /locus_tag="OQS10_003663"
     CDS             complement(<1..1780)
                     /locus_tag="OQS10_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS2294591.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OQS10_003664"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OQS10_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS2294592.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OQS10_003665"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OQS10_003665"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS2294593.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OQS10_003666"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OQS10_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS2294594.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OQS10_003667"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OQS10_003667"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS2294595.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="OQS10_003668"
     CDS             complement(5968..7005)
                     /locus_tag="OQS10_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS2294596.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="OQS10_003669"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="OQS10_003669"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS2294597.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAJVXP010000169         7720 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVXP010000169 DAJVXP010000000
VERSION     DAJVXP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.169"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..646
                     /locus_tag="OQS10_003670"
     CDS             <1..646
                     /locus_tag="OQS10_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2294598.1"
                     /translation="PHAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICL
                     SLADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASG
                     MRGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDR
                     QLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            897..2459
                     /gene="hpaB"
                     /locus_tag="OQS10_003671"
     CDS             897..2459
                     /gene="hpaB"
                     /locus_tag="OQS10_003671"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS2294599.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2477..2989
                     /locus_tag="OQS10_003672"
     CDS             2477..2989
                     /locus_tag="OQS10_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS2294600.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3382..5532
                     /gene="btsT"
                     /locus_tag="OQS10_003673"
     CDS             3382..5532
                     /gene="btsT"
                     /locus_tag="OQS10_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS2294601.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5582..5785
                     /locus_tag="OQS10_003674"
     CDS             5582..5785
                     /locus_tag="OQS10_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS2294602.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5796..6752
                     /gene="yjiA"
                     /locus_tag="OQS10_003675"
     CDS             5796..6752
                     /gene="yjiA"
                     /locus_tag="OQS10_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS2294603.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6830..>7720)
                     /locus_tag="OQS10_003676"
     CDS             complement(6830..>7720)
                     /locus_tag="OQS10_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS2294604.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         1786 a         2104 c         2020 g         1810 t
ORIGIN      
        1 acctcatgcg tttattacgg agtctgacgc cgacggtcat gtgttgacgg tacgggaaga
       61 tctgatctgg cccctgctgg aagttcttta tccaggcact cgggaaacct tcggcctgcc
      121 ggggatttgc ctgtcactgg cagataaacc cgacgaactg gcggcgctgg aacactattg
      181 gcaactgata gagcgggaat cggtggaaca actgcctgga cgggaacaca ccctgacgct
      241 actggcacag gcagtgttca ccctactgct gcgtaatgca aaactcgacg accatgccgc
      301 cagcgggatg cgcggagaat taaaactgtt ccagcgtttt aatatgctga ttgaaagcca
      361 ttttcatcag cactggacag taccggatta cgctaacgaa ctgcatatca ccgaatcacg
      421 cctcacggac atctgccgcc gctttgccaa ccgtccgcca aaacggttga tttttgacag
      481 gcagctgcga gaagccaagc ggctgctgct gttttctgat aacgccgtga acaatattgc
      541 ctggcaactc ggttttaagg atccagctta ttttgcgcgc ttttttaatc gcttagtcgg
      601 ttgctcgccc agtgcttatc gtgccaaaaa agtacctgtg acgtgaaaat cccttagcct
      661 taacgggaaa ccaggcacca cctgctattc ccctttctcc gcctggagca aggggattta
      721 gtaacgtcaa aaatcgctaa aagcgaccgc gatcacaaaa ccgtacccga cctgaaaagt
      781 accagccatt gtcccaaaag tgtcttcccg taatcgcccc tgcaagcttc aattgaataa
      841 caaaaaaaac acacaaaatt aacaatcgta ttccgattaa tactgtagag gtcgacatga
      901 aaccagaaga tttccgcgcc agtacccaac gtccgttcac cggggaagag tatctgaaaa
      961 gcctgcagga tggtcgcgag atctatatct atggcgagcg agtgaaagac gtcactactc
     1021 atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact gtacgacgcg ctgcacaaac
     1081 cggagatgca ggactctctg tgctggaaca ccgacaccgg cagcggcggc tatacccata
     1141 aattcttccg cgtggcgaaa agtgccgacg acctgcgcca gcaacgtgac gccatcgctg
     1201 agtggtcacg cctgagctat ggctggatgg gccgtacccc agactacaaa gctgctttcg
     1261 gttgcgcact gggcgcgaat ccgggctttt acggtcagtt cgagcagaac gcccgtaact
     1321 ggtacacccg tattcaggaa actggcctct actttaacca cgcgattgtt aacccaccga
     1381 tcgatcgtca tttgccgacc gataaagtga aagacgttta catcaagctg gaaaaagaga
     1441 ctgacgccgg gattatcgtt agcggtgcga aagtggttgc caccaactcg gcgctgactc
     1501 actacaacat gattggcttc ggctcggcac aagtaatggg cgaaaacccg gacttcgcac
     1561 tgatgttcgt tgcgccaatg gatgccaatg gcgtcaaatt aatctcccgc gcctcttatg
     1621 agatggtcgc gggtgctacc ggctcgccgt acgactaccc gctctccagc cgcttcgatg
     1681 agaacgatgc gattctggtg atggataacg tgctgatccc atgggaaaac gtactgatct
     1741 accgcgattt cgatcgctgc cgtcgctgga caatggaagg cggttttgcc cgtatgtatc
     1801 cgctgcaagc ctgtgtgcgc ctggcagtga aactcgactt cattacagca ctgctgaaaa
     1861 aatcactcga atgtaccggc accctggagt tccgtggtgt acaggccgat ctcggtgaag
     1921 tggtggcgtg gcgcaacacc ttctgggcat tgagtgactc gatgtgttct gaagcgacgc
     1981 cgtgggtcaa cggggcttat ttaccggatc atgccacact gcaaacctat cgcgtactgg
     2041 caccaatggc ctacgcgaag atcaaaaaca ttatcgaacg caacgttacc agtggcctga
     2101 tctatctccc ttccagtgcc cgtgacctga acaatccgca gatcgaccag tatctggcga
     2161 agtatgtgcg cggttcgaat ggtatggatc acgtccagcg catcaagatc ctcaaactga
     2221 tgtgggacgc cattggcagc gagtttggtg gtcgccacga actgtatgaa atcaactact
     2281 ccggtagcca ggatgagatt cgcctacagt gtctgcgcca ggcacaaagc tccggcaata
     2341 tggacaagat gatggcgatg gttgatcgct gcctgtcgga atacgaccag aacggctgga
     2401 ctgtgccgca cttgcacaac aacgacgata tcaacatgct ggataagctg ctgaaataac
     2461 gcagcaggag gttaagatgc aattagatga acagcgcctg cgctttcgtg acgcgatggc
     2521 cagcctgtcg gcagcggtaa atattatcac caccgagggc gacgccggac aatgcgggat
     2581 tacggcaacg gccgtctgct cggtcacgga tacaccacca tcgctgatgg tgtgcattaa
     2641 cgccaacagt gcgatgaacc cggtttttca gggcaacggt aagttgtgcg tcaacgtcct
     2701 caaccatgag caggaactga tggcacgcca cttcgcgggc atgacaggca tggcgatgga
     2761 agagcgtttt agcctctcat gctggcaaaa aggtccgctg gcgcagccgg tgctgaaagg
     2821 ttcgctggcc agtcttgaag gtgagatccg cgatgtgcag gcaattggca cacatctggt
     2881 gtatctggtg gagattaaaa acatcatcct cagtgcagaa ggtcacggac ttatctactt
     2941 taaacgccgt ttccatccgg tgatgctgga aatggaagct gcgatttaag ctccagcccc
     3001 ttcacgcatt cgtcgaaggg gcatttttat tgtgatctca atcacatatc cacccgatga
     3061 ataattcttg cccaaccgct caccgccatt cctgaccgct tacgtcccta aaataaccac
     3121 tcagttattt accttacttt acgcgcgcgt aactctggca acatcactgc aggatagcgg
     3181 tcaatttacc tcctcaaacg caacgcaaac ctagaacggc ttcggcctat gattaacaaa
     3241 ttactctatg aattttggtt tgcaggaagg cagcaagtga gtgaatcccc gggagcttac
     3301 aacagtaagt gacaggggtg aacgaacgca actgccgcac ctgtaagcca aaagacgacg
     3361 agtaaaatgc caggttttac tatggatacg aaaaaactat tcaagcacat accctgggtg
     3421 attctcggaa tcatcggtgc attctgcctc gcggtagttg cattacgtcg gggggagcac
     3481 gtcagcgccc tgtggatcgt ggtcgcctct gtgtcggtgt atctggtggc gtatcgctac
     3541 tacagtctgt acatcgccca gaaggtgatg aaactcgacc ccacgcgcgc gacgcctgcg
     3601 gttattaaca acgacggtct gaactacgtt ccgaccaacc gttacgtgtt gtttggtcac
     3661 cacttcgccg ctatcgccgg tgctggtccg ctggtcggtc cggttctcgc cgcacagatg
     3721 ggccacctgc ctggcacgct gtggctgctg gcgggggtag tactggccgg tgcggttcag
     3781 gactttatgg tgctgtttat ctcctctcgc cgtaacggcg catctctcgg tgagatgata
     3841 aaagaagaga tgggaccagt accggggact atcgcgctgt ttggctgttt cttaatcatg
     3901 atcatcatcc ttgccgtcct ggcgctgatt gtggttaaag ccctggccga aagtccgtgg
     3961 ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt ttatgggtat ctacatgcgc
     4021 tttatccgtc cgggacgtgt gggtgaagtc tctgtcattg gtatcgtgct gctggttgcc
     4081 tctatctact tcggtggcgt gattgctcac gatccgtact ggggtccggc actgaccttt
     4141 aaagacacca ccattacctt cgcgctgatt ggctatgcgt ttgtttccgc attgctgccg
     4201 gtgtggctga tcctcgcacc gcgcgactat ctggcaacct tcctgaaaat cggcgttatc
     4261 gtcggcctgg cgctgggtat cgtggtgctg aacccggaac tgaaaatgcc tgcgatgacc
     4321 cagtacattg acggtactgg cccgctgtgg aaaggtgctc tgttcccgtt cttgttcatt
     4381 accatcgcct gtggtgcggt atctggcttc cacgcactga tctcttccgg tacgacgccg
     4441 aaactgctgg ctaatgaaac cgacgcgcgt ttcattggct acggcgcaat gctgatggag
     4501 tccttcgtgg cgattatggc gctggttgct gcgtccatca tcgaaccggg tctttacttc
     4561 gcgatgaaca ccccgcctgc tggccttggc atcaccatgc ctaacctgca tgaaatgggt
     4621 ggcgagaacg cgccgatcat catggcgcag ctgaaagacg ttaccgcaca cgcggcagcg
     4681 accgtcagct cttggggctt cgtgatttcg ccagagcaga tcctgcaaac cgcgaaagac
     4741 attggtgagc cttctgtcct gaaccgtgca ggtggtgcgc caacgctggc ggtaggtatc
     4801 gctcacgtgt tccacaaagt gctgccgatg gctgacatgg gcttctggta tcacttcggt
     4861 attctgttcg aagccctgtt catcctgacc gcgctggatg cgggtacccg ttctggccgt
     4921 tttatgctgc aagacctgct gggtaacttc atcccgttcc tgaaaaaaac cgactctctg
     4981 gttgccggta tcatcggtac tgcgggctgt gtgggtctgt ggggctacct gctgtatcag
     5041 ggcgtggtcg atccgctggg cggcgttaag agcctgtggc cgctgttcgg tatctccaac
     5101 cagatgctgg cagccgtagc gctggtactg ggcaccgttg tgctgattaa gatgaagcgc
     5161 acccaataca tctgggtaac tgttgttccg gctgtatggc tgcttatctg caccacctgg
     5221 gcgctgggtc tgaaactgtt cagcaccaac ccgcagatgg aaggcttctt ctacatggca
     5281 agccagtaca aagagaagat tgctaacggt actgacctga cggcgcagca gattgccaac
     5341 atgaaccaca tcgttgtgaa caactacacc aacgcaggcc tgagtattct gttcctgatt
     5401 gtggtgtaca gcatcatctt ctacggtttc aaaacctggc ttgcggtgcg taacagcgac
     5461 aaacgtactg acaaagaaac tccgtacgtt ccaatcccgg aaggcggcgt gaagatctct
     5521 tcgcaccact aaccgtgttt agccccgctt cggcggggct ttgttcttat cagagtgaac
     5581 tatgtttggt aacttaggac aggcaaaaaa atatctcggc caggcggcga agatgttgat
     5641 tggcattcca gactacgaca actacgtcga gcatatgaag accaaccatc ccgacaagcc
     5701 gtacatgagc tatgaagaat tcttccgcga acgccagaac gcgcgctacg gcggcgatgg
     5761 taaaggcggt atgcgctgtt gttaatggag agagcatgaa cccgattgca gttaccctac
     5821 tcaccggttt tttaggcgcg ggcaaaacca ccctgctgcg ccatattctt aacgaacaac
     5881 acggctacga gattgccgtg attgaaaacg aattcggcga agtctccgtt gatgatcaac
     5941 tgattggcga ccgcgccacg cagatcaaaa cgctgaccaa cggctgcatc tgctgttcgc
     6001 gctccaacga gctggaagac gcactactcg acctgctgga taatctcgac aagggcaata
     6061 ttcagttcga ccgtctggtc attgaatgca ccggcatggc cgatcccggc ccgattattc
     6121 agaccttctt ctcccatgag attttatgcc agcgctacct gctggacggg gtgattgcgc
     6181 tggtggatgc ggtacatgcc gatgagcaga tgaaccagtt caccatcgcc cagtcccagg
     6241 ttggctacgc cgaccgtatt ctgctgacga aaaccgacgt cgcaggcgaa gcagagaaac
     6301 tgcgtgaacg cctggcgcgc atcaacgccc gcgcaccggt ctacaccgtt acccacggcg
     6361 atatcgacct gggccttctg ttcaacacca acggttttat gctggaagaa aacgtcgtca
     6421 gcaccaaacc gcgtttccac tttatcgcgg acaaacaaaa cgatatttcg tcgattgtgg
     6481 tggaactgga ttacccggta gatatcagcg aagtttcccg ggtgatggaa aacctgctgc
     6541 tggaatcggc ggataaactg ctgcgttaca aagggatgct gtggattgac ggcgaaccta
     6601 accgcctgct gttccagggc gtccagcgcc tctacagcgc cgactgggac cgcccgtggg
     6661 gcgatgaaaa accgcatagc acgatggtgt ttatcgggat tcagttgccg gaggatgaga
     6721 ttcgggcggc gtttgcggga ctcaaaaagt aaccagccct ccaacatcac aatacctgag
     6781 actttatcac cctcatattc ctccagaata tgagggcatt cgggcttaat tatctctgaa
     6841 cgtgcaacac ttcctgcgac tccttatgcg cctccacatc ctgcttcgtt aaccagagcg
     6901 acttacgacc aaaattttcg tacattttca gctgatcttc atagtgctga tcaactgttc
     6961 catcgggagc aataaaccca ctctgaccgg gtgcgaccac atcccaggca agcaccggac
     7021 gatcgcttgt cgttggtgag aaaacaatca tatcgttttc tgttccacgg ttttgatact
     7081 ccgcctgatg acgcgtttct tccgctgcgg cctgcggtac accaaagaaa ttatttgcac
     7141 ggaacgttaa ggccattgca ggtgttttcc agttactcac attattgcca tagcgtttgg
     7201 aaagagtctc ccaggtatct tccagcgcag ccaacacaac ctcctgctgt ggtttcccag
     7261 caaacagatc aaccgcctgt gggattggtg atttgtctcc ctgcaccgcc tcatacaaaa
     7321 tttttgctcc aacacttata ttcagcgaac cagttgggcc gtcctgggtt gtttcgtagc
     7381 cactggcgct gtaccactta tcaaatggca taggtacggc agccactacg gtacgcttca
     7441 acatactggt cagccaaacg ttcaggatgg cagagcctgg ctgctgccag gttttaccat
     7501 catcattaag caaattgatg ccatcccaac gtgttaatgt atccaccaac tgacggcgcg
     7561 gatcgctctg tgtcaaacca gatgtcgctg cttgcagagt aggtaaaaaa agcctcaggt
     7621 taagatcctg acgactggtt tggcgaataa catcccatgc ctgatcagca gttaagcgtg
     7681 gcttttgctc aagcagtcgg tcgatctccg taacgcgatc
//
LOCUS       DAJVXP010000170         7613 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVXP010000170 DAJVXP010000000
VERSION     DAJVXP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.170"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..96
                     /locus_tag="OQS10_003677"
     CDS             <1..96
                     /locus_tag="OQS10_003677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2294605.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OQS10_003678"
     CDS             complement(152..487)
                     /locus_tag="OQS10_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS2294606.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OQS10_003679"
     CDS             complement(491..835)
                     /locus_tag="OQS10_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS2294607.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OQS10_003680"
     CDS             complement(837..1010)
                     /locus_tag="OQS10_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294608.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OQS10_003681"
     CDS             1111..1296
                     /locus_tag="OQS10_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294609.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OQS10_003682"
     CDS             1257..1376
                     /locus_tag="OQS10_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS2294610.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OQS10_003683"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OQS10_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS2294611.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OQS10_003684"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OQS10_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS2294612.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OQS10_003685"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OQS10_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS2294613.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OQS10_003686"
     CDS             4176..4544
                     /locus_tag="OQS10_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCS2294614.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OQS10_003687"
     CDS             complement(4652..5053)
                     /locus_tag="OQS10_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS2294615.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OQS10_003688"
     CDS             5295..5588
                     /locus_tag="OQS10_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS2294616.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OQS10_003689"
     CDS             5660..6319
                     /locus_tag="OQS10_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS2294617.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OQS10_003690"
     CDS             6411..6857
                     /locus_tag="OQS10_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS2294618.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7613)
                     /gene="hlyE"
                     /locus_tag="OQS10_003691"
     CDS             complement(7064..>7613)
                     /gene="hlyE"
                     /locus_tag="OQS10_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS2294619.1"
                     /translation="DGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSY
                     FQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFT
                     TLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKM
                     INTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1955 a         1710 c         1771 g         2177 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tca
//
LOCUS       DAJVXP010000171         7491 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVXP010000171 DAJVXP010000000
VERSION     DAJVXP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.171"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OQS10_003692"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OQS10_003692"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS2294620.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OQS10_003693"
     tRNA            647..722
                     /locus_tag="OQS10_003693"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OQS10_003694"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OQS10_003694"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS2294621.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OQS10_003695"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OQS10_003695"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS2294622.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OQS10_003696"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OQS10_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS2294623.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OQS10_003697"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OQS10_003697"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS2294624.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OQS10_003698"
     CDS             complement(5917..6249)
                     /locus_tag="OQS10_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS2294625.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OQS10_003699"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OQS10_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS2294626.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1869 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAJVXP010000172         7453 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVXP010000172 DAJVXP010000000
VERSION     DAJVXP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.172"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..2301)
                     /gene="ppsA"
                     /locus_tag="OQS10_003700"
     CDS             complement(<1..2301)
                     /gene="ppsA"
                     /locus_tag="OQS10_003700"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS2294627.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLME"
     gene            2634..3467
                     /gene="ppsR"
                     /locus_tag="OQS10_003701"
     CDS             2634..3467
                     /gene="ppsR"
                     /locus_tag="OQS10_003701"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS2294628.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3624..4670
                     /gene="aroH"
                     /locus_tag="OQS10_003702"
     CDS             3624..4670
                     /gene="aroH"
                     /locus_tag="OQS10_003702"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS2294629.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            4802..4993
                     /gene="hemP"
                     /locus_tag="OQS10_003703"
     CDS             4802..4993
                     /gene="hemP"
                     /locus_tag="OQS10_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS2294630.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(4997..6433)
                     /locus_tag="OQS10_003704"
     CDS             complement(4997..6433)
                     /locus_tag="OQS10_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS2294631.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6496..7209)
                     /gene="ydiV"
                     /locus_tag="OQS10_003705"
     CDS             complement(6496..7209)
                     /gene="ydiV"
                     /locus_tag="OQS10_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS2294632.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1893 a         1983 c         1769 g         1808 t
ORIGIN      
        1 ttccatcaac catgcggcaa agtcttcgtg gtcggacgga ccctgaccgc aaatcccgac
       61 atatttgccc tgtttcttcg cggcacggat agccatcgac agcagtgctt tcaccgcatc
      121 gttgcgctca tcgaacaatt cagacaccac gccggagtca cggtccagac cgagcgccag
      181 ctgcgtcata tcgtttgagc caattgagaa gccgtcgaaa tattcgagga actgctcggc
      241 cagcaaggcg ttggacggga tttcacacat catgatgatt ttcagcccgt tctcaccacg
      301 cttcagcccc tgacgcgcca gttcctcaac caccgctttc gcctgatcta cggtacgcac
      361 gaacgggatc atgatctcaa cgttggtcag ccccatgtcg ttgcgcacac gtttcactgc
      421 ttcgcactcc agcgcgaagc agtcgcggaa gctgtcggaa acatagcgtc ccgcgccacg
      481 gaagccgagc atcgggttct cttcatctgg ctcgtaacgc tcaccaccga ccaggttggc
      541 atattcgttc gatttaaaat cagagagacg gacaatgacg cgcttcggat aaaacgcggc
      601 acccagcgtc gcgatccctt cagtcagacg accaacgtaa aattcacgcg gagaatcaaa
      661 acctttcatc atctcgcgga tttcattttg caactgcggt tcctgatcgt caaactcaag
      721 cagtgcgcgt gggtggacgc caatcatacg gttgatgata aattccagac gcgcaaggcc
      781 cacgccttcg ttcggcaggc aggcgaagtc gaaagcacgg tccgggttac cgacgttcat
      841 catcactttc aacggcagat ccggcatcat ttctacgctg gagcttttca cgctaaattc
      901 cagcaactcc gcatagacgt aaccggtatc accttcggca caagaaacag tgacgttctc
      961 accgtctttc atccgttctg ttgcatcacc acagcccact accgccggaa tgcccagttc
     1021 acgagcgatg atcgccgcgt gacaggtacg accgccacgg ttggtgacga tggcagatgc
     1081 tttcttcatg atcggttccc agtccgggtc ggtcatgtca gtgaccagca cgtcaccagg
     1141 ttcgatgcgg ttcatttcgc tgatctcatg gatgactttc accggacccg cgccgatgcg
     1201 atgaccgata gcacggcctt cggcgataat cttaccctgt gaatgcagcg tataacgctc
     1261 catgacctga ccgcgtgagc gcacggtttc cggacgcgcc tgcacaatga acagtttgcc
     1321 agtgtggcca tctttcgccc actcaatatc catcgggcga ccgtagtgtt tctcaatttg
     1381 tacggcctgt tttgccagtt cctgcacttc ttcgttggtc agcgagaaga tgtcacgctg
     1441 ttcctgcggt acgtcttcga ttttaacctg cttgccgtgc tcctgggtcg gcgcgtaaac
     1501 catgcggatt tttttcgacc ccatggtgcg acgcacgata gccgggcgat tcgccgccag
     1561 tgtcggttta tggacgtaaa actcatccgg gttaaccgca ccctgcacga ccatttcccc
     1621 aaggccccat gcggaagtga taaacaccac ctggtcaaag ccggattcgg tatcaatgga
     1681 gaacatcacg ccagaagatg cgaggtcgga gcgcaccatc cgctgaacac cggcagagag
     1741 cgccacgcca cggtgatcgt aaccctggtg cacacgataa gagatggcgc gatcgttaaa
     1801 cagagaagca aatacatgct tcactgccac gagaacggcg tcaaaaccct gaacgttgag
     1861 gaaggtttcc tgctgaccgg caaaagaagc gtccggcata tcttctgcgg tggcggagga
     1921 gcgcaccgca aaagaggcgt tttcgtcatc ggcggaaagc tgtgcatagg cttcgcggat
     1981 ggcgttttcc agctcaggct ggaagggagt gtcgataatc cactggcgga tttgcgcgcc
     2041 cgctttcgca agctgagtaa catcgtcaat atccgtttta tccagcagtt cataaatgcg
     2101 ctggtttacg ccgctttggt ccagaaactg gttaaacgcg tcggcggttg tggcgaaacc
     2161 attcggaacg gaaacaccca ttccggaaag gttagtaatc atttcaccca gggaggcatt
     2221 tttgccccca accctgtcta catcattcat gccgagttgg ttataccaaa gcaccagcgg
     2281 tgacgagcca ttgttggaca tcgaacaatc cttttgtgat aaatgaacgg tttgagaaac
     2341 acatttctgc gcatttatct ttgcatattt aaccggatga aaaaaacggt gaatcgttca
     2401 agcaaatata tttttttact ttttaagact gatcccagcg ttgcgcaaat ctgctctctt
     2461 cgacaatttc cacataaacc atcggtataa acgaaaccat aaaaatgaaa tgctgttttc
     2521 ataaaaaata aaattgaagg ttcattttat aaaccagaca taacgtccta cgcttctgtg
     2581 cgtttttaat ttatgctttc atagaattat gtctgcatca cgggaagaac aaaatggata
     2641 atgctgttga tcgccacgtt ttttatattt ctgatggtac ggcaataact gcggaggtat
     2701 taggacacgc agtaatgtca caatttcccg tcactatcag cagcattacg ctgccgtttg
     2761 tcgaaaatga gagccgtgca cgggcagtga aggatcagat tgacgcaatt tatcaccaga
     2821 caggtgtgcg cccgctggtc ttctactcca tcgtgttgcc ggagattcgc gccatcatct
     2881 tgcaaagtga aggcttttgc caggatatcg ttcaggcgct ggttgccccg ctacaacaag
     2941 agatgaaact ggatccaacg ccgattgctc atcgtaccca tggccttaac cctaataatc
     3001 tcaataaata tgatgcgcgc attgcggcga ttgattacac cctcgcccac gatgacggca
     3061 tttcgttgcg caatctggat caggctcagg tgatcctgct cggtgtttct cgctgtggta
     3121 aaacccccac cagtctttat ctggcaatgc aatttggtat ccgcgcggca aactacccct
     3181 ttattgccga cgatatggat aatctggtgc tacccgcgtc gctcaaaccg cttcagcata
     3241 aattgttcgg cctgactatc gacccggaac gtctggcggc gattcgcgag gaacgtcggg
     3301 agaacagtcg ctatgcctcg cttcgtcagt gcaggatgga agtcgcggaa gtggaagcgt
     3361 tgtaccgtaa aaatcagatc ccgtggatta acagtaccaa ttattcggta gaagagattg
     3421 ccaccaagat cctcgatatc atgggcctta gtcgccgaat gtactagaga actagtgcat
     3481 tagcttattt ttttgttatg atgctaacca cccggcgagg tgtgacacac ctcgcacttg
     3541 aaatcagcag cgattggttt atcgtgatgc gcatcacttc ccggcagtcc tgccgtagaa
     3601 gcaacaaatt tctgagactt gtaatgaaca gaactgacga actccgtact gcgcgtattg
     3661 agagcctggt aacgcccgcc gaactcgcgc tacggtatcc cgtaacgcct ggcgccgcca
     3721 cccatgtcac cgactcccgc cgcagaattg aaaaaatact caatggtgaa gataagcgac
     3781 tgttggtcat tattggcccc tgctcgatcc acgatctcac cgctgcaatg gagtacgccc
     3841 cccgtctgca gtcgctgcgc aaccagtacc agtcacggct ggaaatcgta atgcgcacct
     3901 attttgaaaa accacgaact gttgtcggct ggaaaggact aatctccgat ccagatttaa
     3961 acggcagcta tcgggtaaat cacggtctgg agctggcgcg caaattactt ttacaggtaa
     4021 atgagctggg cgtcccaacc gcgactgagt tcctcgatat ggtgaccggt cagtttattg
     4081 ctgatttaat cagttggggc gcgattggcg cacgtactac cgaaagtcag atccaccgtg
     4141 aaatggcttc ggcactctcc tgtccggtag gttttaaaaa tggtaccgat ggcaatacgc
     4201 ggattgctgt ggatgctatc cgcgcagccc gcgccagcca tatgttcctc tcgccagaca
     4261 aaaatggtca gatgaccatc tatcagacca gcggcaaccc gtatggccac attattatgc
     4321 gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc cgcagcctgc gatacgctgc
     4381 acgagtttga tttacctgaa catctggtgg tggatttcag ccacggtaac tgccagaagc
     4441 agcaccgtcg ccagttagaa gtttgtgagg atatttgtca gcaaatccgc aatggctcta
     4501 cggcgattgc tggaattatg gcggaaagtt tcctgctcga aggaacgcaa aaaatcgtcg
     4561 gcagtcagcc gctcacttac ggtcaatcca ttaccgaccc gtgtctgggc tgggaagata
     4621 ccgaacgcct ggtcgaaaaa ctcgcctctg cggtagatac ccgcttctga atgcgtgccc
     4681 attcctgacg gaatgggcat ttctgcgcaa cttgttgtct tctcaacaaa ttactgcttg
     4741 ctctggcctg ccataatatt gataataaga atcattgtta tatcaattat tattaatctt
     4801 tatgcgttat acggatagca gaaaactcac gcctgaaacg gacgccaatc acaaggccgc
     4861 ttccccgcag cccatccggc gaattaccag ccagacactg ttaggtccgg atggcaaact
     4921 gattatcgat catgacgggc aagaatatct gctccgtaaa actcaggctg gcaagctgct
     4981 gttaactaag tagcctttaa ctcgagcagc tgacttccag ccgtttaccc cagtcaggtg
     5041 gacgactgac atagtcatca tctctatcgc tgaaaggatt tcgcaacgcc ccatgcaggc
     5101 ggtgcaattc cgtcatatca cccttttctg ccgcttcaat cgcccgttgc gccaaccagt
     5161 tgcgcaatac cagagcgggg ttaacgcttt gcatcaactg ctgacgctca ctatcgctaa
     5221 cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca gccatcaaat gccgcacgat
     5281 caataaactc atcacgtagc ggtgacgccg tgctgtgctg ctcggtcaga ctcagcatgc
     5341 ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag actgaataat tcattcagta
     5401 gcgcgttatc ctctttttgc tccgtgatga agcccagttt ctgccgcatc cgttgtccat
     5461 aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc attcagggca tctacggcaa
     5521 caaatggtga caatgtctgc gccagacgct gtaaattcca caacgcgaca gcaggttgat
     5581 tatcaaaact ataacgccct tgatgatccg agtgattaca aataaaaccg ggttcgtaat
     5641 catcaagaaa accaaacggc ccgtaatcaa gcgtcagtcc cagcagcgac atgttgtcgg
     5701 tattcatcac cccatgtgca aagccgaccg tctgccattg ggcaattaac gacgcggtac
     5761 gtgcgacaac atcgctaaac cagagacggt atttgtcctc atcatcttca agatgtgacc
     5821 agtaatgacg aatagcaaag tcagctaact gacgaacctt ttccgactcg cggcggtagt
     5881 aaaaatgttc gaaatgacca aagcgcagat gacttggtgc cacacgcatc agcatcgcgc
     5941 ctggctccgc cgtttcccga tacactggcg aatcgctggt gacgatactt aacgcgcggg
     6001 tcgtcggaat gcccagataa tgcatcgcct cactggcgag actttctcgt atcgtcgaac
     6061 gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt caggccagca cctttcagat
     6121 gccagtccat tgtagtgcca tcagcaagca gttgttcgcc gagtaaaatg ccgcgcccat
     6181 cacccagttg gcccgcccag acgccgaact gatgaccact gtaaacctgc gccagtggtg
     6241 acataccagg cagtagggct tcgccgcccc agacacctgc gccattttta aacagcgacg
     6301 atggaatact cagcgtgtta gccagttcgg tattatgcca aatcagccgg gcattattta
     6361 aaggcgtagg ggaaagtgct gtataggttt ctggcaattc atcgcgccag cgggtaacaa
     6421 aagacagggt catagatcct cctgtttgat agtgtagacg gttactctcg tcttaaacac
     6481 cagcaaacag aagggttatc gctgaaccaa cgtcgttatc tgatttatgg gaacggcagg
     6541 ccacaggcat ccctggagag catggaaatc gaagggggta atctgtgcca atatttctgc
     6601 cgtatcaatg cctcccgcaa tgatgcaatt gcaacaaggg gaaatttgcg cctggatcgc
     6661 acgaataaac ggttcgaagg agcggtgcgt gatttgttgc tgaataaaac ttttatccag
     6721 catgacgcgg gtgaaaagac catcaaaaac cgctttcatt gtgctattgc ctgctcccaa
     6781 attacctaac acaaggggat acacttgcga taaggataaa agaccgcgat tatcttttcc
     6841 ttcattgaga tgcgggtaat tctcattaat caacagttca ataaacggat attttaatag
     6901 ttcgcctgcg taattatccc tctctaataa taatgtagcc acttgtggcg ttaaattaag
     6961 ccaggcaaag agtttatgtt gaataaaaaa atgttggcat gattttagta attccaactg
     7021 ctctgaaaat aattgccaat gttgctcctc ggtaagttgc gctataaccc gactcgtcgg
     7081 gatacgcacc gtgccatctt cactggagaa atgggtaatt aactcaacgc cgacaaggac
     7141 ttgctggtta tctcttatcg gcaggaaata gcaatcagaa tgataaagat tctccaaaaa
     7201 aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca gtatccggct aaaagggatg
     7261 aggccataag acataacaat tacagaagga gtaactttca tttgttccat gttaaccact
     7321 ttttcagggg tccttttttt agattatcct gattataaac gaataatcca tttacggaat
     7381 ttttgtctgc aaaatactac tgtattcagg agtaaatgga cggcgatgta tcatcgccgt
     7441 ccatttttcg aca
//
LOCUS       DAJVXP010000173         7428 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVXP010000173 DAJVXP010000000
VERSION     DAJVXP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.173"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..98)
                     /locus_tag="OQS10_003706"
     CDS             complement(<1..98)
                     /locus_tag="OQS10_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCS2294633.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
     gene            complement(123..1223)
                     /locus_tag="OQS10_003707"
     CDS             complement(123..1223)
                     /locus_tag="OQS10_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS2294634.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="OQS10_003708"
     CDS             complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="OQS10_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS2294635.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2371..2937)
                     /locus_tag="OQS10_003709"
     CDS             complement(2371..2937)
                     /locus_tag="OQS10_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS2294636.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3153..4889
                     /gene="argS"
                     /locus_tag="OQS10_003710"
     CDS             3153..4889
                     /gene="argS"
                     /locus_tag="OQS10_003710"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS2294637.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5066..5428
                     /locus_tag="OQS10_003711"
                     /pseudo
     CDS             5066..5428
                     /locus_tag="OQS10_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5432..6502)
                     /locus_tag="OQS10_003712"
                     /pseudo
     CDS             complement(5432..6502)
                     /locus_tag="OQS10_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="OQS10_003713"
     CDS             complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="OQS10_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCS2294638.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1946 c         1776 g         1786 t
ORIGIN      
        1 tcttctgccc atgcggggag cggtgctgcc gatgtcacca cctgcgctcc agcggcaata
       61 ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag
      121 gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc
      181 agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca
      241 ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca
      301 agtttttcag cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca
      361 ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt
      421 accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc
      481 gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc
      541 tgtttgccac tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg
      601 cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc
      661 tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca
      721 atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc
      781 tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac
      841 gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga
      901 gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta
      961 gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca
     1021 cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa
     1081 gatttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct
     1141 ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac
     1201 ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca ccggaatcat
     1261 tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa
     1321 ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca
     1381 gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga
     1441 atataaaaaa tattattagc acaccgctat gttcactgtc acccgctcaa cagatcacgg
     1501 ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg
     1561 gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc
     1621 tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc
     1681 gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc
     1741 ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag
     1801 tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg
     1861 gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc
     1921 agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac
     1981 atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc
     2041 attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa
     2101 cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag
     2161 caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc
     2221 acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat
     2281 tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct gtaacagcaa
     2341 atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg caacgatctc
     2401 ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg
     2461 attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga tccccggtag
     2521 gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc
     2581 cagcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca
     2641 cggtagctcc acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa
     2701 cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa
     2761 cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc
     2821 agtcagcgga gtgatattca accccagacg gagggaaagt tcatctaatg cgtgggtaaa
     2881 tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat
     2941 cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc aaccaaccgc
     3001 tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct
     3061 gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat
     3121 ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa
     3181 aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc
     3241 attcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa
     3301 aactgggtat gacaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg
     3361 gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg
     3421 cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gtttctacgc
     3481 cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcatg
     3541 tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg
     3601 gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga
     3661 ttgcatggct ggaaaagcag cagcaggaaa acgccggtga tgaaatggag ctggctgacc
     3721 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     3781 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     3841 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
     3901 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     3961 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     4021 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     4081 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     4141 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     4201 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     4261 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     4321 aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg gcagaaaaga
     4381 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     4441 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     4501 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     4561 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     4621 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     4681 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     4741 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     4801 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
     4861 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
     4921 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
     4981 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
     5041 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
     5101 ctcttgccag gcttacgcag aagatacggt tatagaaaac gaccatgaca tttcagccat
     5161 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     5221 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
     5281 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg aacaacttaa
     5341 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
     5401 cctttcaaat gaggacgttc aggatttaat gtacatcgtt ccacgtcgct tcgacaacgg
     5461 tattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc
     5521 gtatccacca ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca
     5581 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     5641 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     5701 cgatgacccc cgcggcggta aaaatcacca gcgccggaat ttgtgccacc agaccatcgc
     5761 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     5821 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     5881 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca
     5941 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt
     6001 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     6061 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca
     6121 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc
     6181 ctgccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa
     6241 gtgccagacg caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg
     6301 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     6361 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     6421 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca
     6481 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     6541 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     6601 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     6661 ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     6721 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     6781 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     6841 ttattgacaa tgacatcagc tttcggcaca tcggccatca tccgacgccg tgcagcagct
     6901 cgctgcatct gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca
     6961 tcacgaatat cctgccgcga catacgcagc tttttcaggt ggctgaagat ttggaaaaag
     7021 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagcccaacc
     7081 agatccatcg cattacccat ggcggtagtc ggagactcgg ccattaagcg catcatctgc
     7141 ggccagtgat gccagagata aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     7201 gccttaagca actccgcgcc agtctgtgcc gagaacatcc gtttaatgcc cggtagcggg
     7261 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc ccccagcatg
     7321 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg
     7381 gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtca
//
LOCUS       DAJVXP010000174         7412 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVXP010000174 DAJVXP010000000
VERSION     DAJVXP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.174"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            353..1306
                     /locus_tag="OQS10_003714"
     CDS             353..1306
                     /locus_tag="OQS10_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS2294639.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1306..1602
                     /locus_tag="OQS10_003715"
     CDS             1306..1602
                     /locus_tag="OQS10_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294640.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            1926..2216
                     /locus_tag="OQS10_003716"
     CDS             1926..2216
                     /locus_tag="OQS10_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294641.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2437..2652
                     /locus_tag="OQS10_003717"
     CDS             2437..2652
                     /locus_tag="OQS10_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294642.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            2799..3104
                     /locus_tag="OQS10_003718"
     CDS             2799..3104
                     /locus_tag="OQS10_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294643.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3580..4290
                     /locus_tag="OQS10_003719"
     CDS             3580..4290
                     /locus_tag="OQS10_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294644.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4315..4521
                     /locus_tag="OQS10_003720"
     CDS             4315..4521
                     /locus_tag="OQS10_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294645.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4620..6800
                     /locus_tag="OQS10_003721"
     CDS             4620..6800
                     /locus_tag="OQS10_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS2294646.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            6957..7412
                     /locus_tag="OQS10_003722"
                     /pseudo
     CDS             6957..7412
                     /locus_tag="OQS10_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2443 a         1301 c         1593 g         2075 t
ORIGIN      
        1 aggtgattca gatattaaga agtatccctg tgaacaataa atgaccaatc agtaattatt
       61 ctgtgaaata attgttgcaa tttataatca catctgttat agtttttata tggggtgata
      121 aaagaactca gaacattaag aggttacaac gttgggaggc gtcagggtat tgacagattt
      181 tcacggcctc ttattcaatc aaggcttttc cttttgctgt taatactcgc gtaatcgctc
      241 aggcttctga gttcctttat cagtccatct tctaattaat tcactccctc tggggttggt
      301 cactatacgt ggtcagccct ttctttgttt tatttttaaa aaggaaaata taatggaaaa
      361 attccacatg tttgatgacc gtatttatca gatgactaat ggcgttaaag ctgccgaccg
      421 tattatttac actgtcatgt tgaatgaaca aaagttttat cacgataagg ggcaatatta
      481 taccccttca tatgctcatc ttggtcagta tgctggaatc acaagaacat ccgtgattgc
      541 atgtatgaaa caacttgagg cacatggttt aatcaagaaa actaacgttg caagcaatca
      601 gtgtgttatt aaagtttatt cacttgatga agtaccggag gttttaacag agcttacagc
      661 taaggaagcc agaaaggcaa agctggagtc taaagttact cagtcagtca agaaagatga
      721 cccactgagt caaaattctt tacccactga gtcaaaattt tttactcagt cagtcaaaat
      781 ctttgacacg tataataaca gtaataacaa gaaagaactt attaataaca ataaagaaga
      841 tgactttgaa gaagaatatt ttaagaaggg agcttcgctc tgtgattcac atgaatgtga
      901 ttctcttggt attgaagagg ctattaaaga gactgttaaa acatctgact tagatgacct
      961 tgatagtgaa gctgctaaag aggtcgttaa aaaggaagat aaaatctctg atgtgattgc
     1021 tagtagtgac cataaaaaca cacagtgccc cgccactgat gatgaattac cggatgttga
     1081 ccttgatgat gaagaagacc ctgaagagga attgaaacgt gaacagcgta aagcagaaat
     1141 taaagaaaga ttcagaaatg cgaagctaaa gaacaaggtt agtgacctcg atgactttga
     1201 tgatagtgac gatgatttta cttttacacc aaaaaagaaa actcacaccg agccaaagaa
     1261 agaaaaagtt aaagtcagac gtgtttatgg tggaggaatg ttttaatgaa aacttataat
     1321 ggcgagagaa aataccgtga ctatttactc agacgttaca gcatttctcg tgaaggttac
     1381 ttgatgaaag atactcacgg ggaagtttac cggattcgac cgaagaaaga agggagggat
     1441 tattatttcg tcgatgatgt gacagattta aagattgacg ctttgaagtt tgcggtgctg
     1501 tatcattacg atttatggga ttcagtccac cagctacgat taaaagacgg tgaccccggt
     1561 aatctgaggg ccaccaatat tatcaaggga aagtgccggt aaggccacca gctatttatt
     1621 tactgaaatc tctatctcaa cacgaagaag tttaagtcga attgtaagct ttgtcttttt
     1681 tgtaatgact cgattcataa cgcaaaacac caatagcaca ataaataccg caaagcccaa
     1741 cgagctaaaa cgatgagatt caataattgc tgtgaaaaaa tcatgcatta attttccttt
     1801 tgtgaaagga aacagcacct gtatcagccc ctacaaccat agtatcatgt aaatgaggta
     1861 ctgacaatat gttaaataga tgttaagatt gtagtatgag taagagattt aaggagattt
     1921 ttacaatgac tcacacagtt acgcgccttt tgccagaggc atttgaacga ctggcagcaa
     1981 tcaaagccaa atatcccgat atgaagcaca cagcgcttgt tggggagatt ttaaaagagt
     2041 cggtaaacct tagccctttc gataacgtta agggcgtcgc agagggttct aaggccatca
     2101 gaatcacaac gggccaactg gaactactca aagcacagtc cgcagtagca ggttgctcaa
     2161 tgattcaggt gttaagcgct aaggtccatc aactggcgga tagattggag gtttaatgtg
     2221 ttataggttc caatattcca cacttggccg ccacctaatc agacagaatt tagcagatgc
     2281 tgaatttgaa gatgtgaccc tcaaagcaaa caaacgatta aaaccctctc aaatccacca
     2341 gcttaaacac gaattaaagc cactggtaat actcgactat aacgaccctg aacagcttga
     2401 gcagggaatt aagcttattt ctgaatgggg tgcttgatga aaactatcac agtacaactt
     2461 cagactaata aagcatttcg ttattttgaa aacttgcttg agttatacga aggatgggga
     2521 agtatccacg gcaaagatga tatctatctt cacttgtcag cccctaacta ttctctgaag
     2581 actccggtta agcaaagctg gttaaaagac tacggtcacc agatggggct attagtctca
     2641 gacttgagct aaaaacagca aaacatatct ctcaggcttt accagacgca ccagaaacgt
     2701 ttttaagccc ctaatcaggg taacttaggg aatcgcctta agtggtctaa aaaaggggtt
     2761 aaaacgtctt agaatgcgat acaggaagga ttttaaaaat gaattaccca atgcaaatta
     2821 tggttgacca agaaaccatg aattggttga ttgacctgaa caggtcgggc tttaatatct
     2881 atttcatgca ttgttttgaa gcgtcaacgt atattgacaa gcatataatc aacgaaatta
     2941 aacttggtga ctatgttcac aagctggaag taacagaccc atacaataag cgctttatca
     3001 ttgaagagtg caatcgactt gatttaagcc ctgagcaatt attgaagctg aatgtattct
     3061 tgacctatca gaatgaatta aacccttaca gcgaggtgat gtaatgacac tcgttattct
     3121 ctggatgact ttagggttat ttgttggttg ctgggtaggt aatacggtaa ataagaattg
     3181 atgttaccaa tctgttaaaa agctgttgac aaataacctt aagcatgaga tgatggtaat
     3241 agggaagagc aactcttcct gaattaaact attaatcatt ctacatcaat gatagttatt
     3301 ctgaaaggtg caatgctttt cgctacatga cctttcaggt tagtctgaga ggtcatctcg
     3361 aaaagtactc ttgttcattc gtttgaatcc tttttgaatc ctttagatgt tttaatttct
     3421 tcaccttatg aatggccctt tataagaaat tatctagggc tattcagctt ttaaaaagca
     3481 tacttggcaa accgcctttt aagtctcttc taagtttaga taggctgtca ggtgtgcttt
     3541 ttaaaaactg aaacacttcc gcttagaaga ggaaaagcaa tgacaaatga tatttacatt
     3601 aatggcaaag tgattgatag cttcagtgac tggtcgccac agactgaaca tccagctatc
     3661 actattcagc gtaaagagca tgacaagcgt attgagcttg agcaatcgct aaagatgtca
     3721 ccaaaaacaa ccatctttgt gtctcctgag cctcaagaga tgcctgatat ttgcaagagt
     3781 gaagcattac ttgagcttga aaagaaatat taccaactgc tgaaagaaca gcgccttaga
     3841 ttagatgacg cttacagcaa agttaccttg atgcaatcag ctattgagcc gtcagaattt
     3901 gagattcagg atgaattgtc acagaaacca tttgtctatt accaatacga agataatgat
     3961 ggttttggta cttttcaaga aaacgtcgca gccgtgatta gtagtcttcc tgctggacat
     4021 cgaattgtga agatggttaa ggcatcaaga ggtgctggtc agtttattta catgactaat
     4081 aagagcgatg aagaactcgc tgaaattgct agacagaaca tcttggcatc acgtaacaag
     4141 caacttgata atgttaagtt gtatctctca agagagcttc aggctatgaa agagcttgtg
     4201 aaagaatatg aagatgctaa gaagattgct atgcaggctg atattgagca gcttacgaag
     4261 atttctagca aatatgcaaa ggctatttaa ttaataatta aaaggtggtg gtgaatgatt
     4321 gatttaacag aacgctatga gcttattaaa gcaattagtg aacgattgaa agaacaaccc
     4381 aacaaaccaa aacgaatcaa atcattcacc tcctctaagc aaaagaatag aatcaggaaa
     4441 atagctaagt ggtgtcttga taaattacca agtgattgtc agattgttgg tgaagatggt
     4501 aattattatc tggttaggta acaagattgg ttgggtgaaa atctacggga gagattatcg
     4561 agtgatatcg gtagtctttc cctttttcgt atatgaacac ttaaagaggt gaaacatgaa
     4621 tgagcaaatt cacagttgat agttttatcg ttgaacttag tttcagtgaa aacgttatta
     4681 agggcttgca aagagttgaa aaggcagctt tgcagtcagc acaaagaatt gaacgcaatt
     4741 tgaatagagc gtttaaggtc gataccaagc aacttgacag caatctgaca agttctttag
     4801 ggcagcttga aaggaagttt aataagacct ttgacaagat tgagcagaga gcaagaagca
     4861 caaaagcatt tcagtttgcc accagattta atgacactgt taatcctcca aaacaatcaa
     4921 ggcaaccacg aattagcgga aacagagcaa tcacagcagc gcatagcgtg aatatgtcta
     4981 agcttggtga tattaatccg ctgatggcta aaatgtttaa agcacagtat tacagcttaa
     5041 gcggtaaggc tggtaatatt ggaagtgaaa agtttaacgc tgaacttgca aaattaaatc
     5101 aatctcttcg tgaaacgttg aataaactca ggtcacagac aaagaccgcc agcattaaca
     5161 atacaagcga cgcatttaat aatcttgcaa gcaatgcgat taaattatct ggtgcatttt
     5221 actctgtgat gggtgctctt aacgcttaca aggccattat gaatgcaggt cttaaacgag
     5281 attccgcaca gagagccgct aagtttgtac ttggtgataa agcatctgaa gctgaggtat
     5341 ttattcgaaa tttagcagac aagacaggtc ttaatatctc tgaagggcta tcaagctacg
     5401 ctaagtttgc agcaggtgct cagggttcta tgtcacagga acagacacag gagctatttg
     5461 gtaatgcaac ggctatgagt cgcttaatgg ggctttctaa cgatgagcta aacggcattt
     5521 tgagagcatt tgagcagatg gcgagcaagg gcaagattca ggctgaggaa ttacgtgggc
     5581 agttaggtga ccgtatggct ggtgcattta agctattcgc tgaagcattg ggtatgactg
     5641 ctacagagct tgatgcagcc atgaaaaacg ggaaggtact ttccgctgat actctcccta
     5701 aagtcgctaa acagatgggc ttgatgattg ataaagcggg tggctgggct gaagtcacta
     5761 agagcactca gacagcttta ggtaagttag caaacaattg ggatgatacg atggtgaaaa
     5821 tcttctcagg ttcacaggat gagctaaacg gcttcttatc cagcctgagt aacctgctta
     5881 gtgaaatggg gatgagttct tctatcgctg gtgatgctat cggtggactt atcgacatgc
     5941 ttaaagctgg tgtagatgat attcgcgtat tcaataacac cattgaaggc tggattttac
     6001 agactaagaa gttctattac agccttgatg acactaaacg aaaactactt gatgaagtcg
     6061 gtgatggttt tatcaacttc gtcaaaggtc ttgctatagc gttatctgca aagacgctat
     6121 tcagtgctac tacaggggtt atgaacctga caagagcaat cacaacgtta ggtacaagag
     6181 ctaatcaggt ggctggtcag gtagctactg gtaaaggtgg tggaaaactg aaaggtgtag
     6241 ctggtggtgt aggtagtgct cttgcaattg gttatgctga tgacggttat gaaacagcgt
     6301 tagcattagc ttcaatgatt ccacaaatca gaggcgtcac tcttggttta tatgcattga
     6361 agaaagcact ggattttatg aatcaggaag tagtcaaaaa cgctaatatg catccgtctg
     6421 gggtaggtgt gggtagtgac tttaatcccg tgtattctgg tgaccctaat aaaccgaata
     6481 tgattaacga aggatggagt agaattttta gttcaatggg tgaattgttt aacaatgcca
     6541 caatgaatat tgcaagattc aatcatccag agttaaacaa cattaagcaa gattcagcat
     6601 taacgagtgc agatattcag ggtctgaggg atgaaattag tgctttgagt aagcgtattc
     6661 aggagccagt gaaagtttca cttggtggtc aagttgtcgt tcgaccagat gaaccatctt
     6721 tctttacttt caatcaatca gctaatgacc agttcacgca ggatatgctc ttaagttcaa
     6781 gctatccaga ggaagaatga tttaacggtg ttctacgtga ataaagcaaa gttaataccc
     6841 acaccaaaaa ttatttttca cgtagctcac gcttaacaga gaaaatacat atatatcaaa
     6901 ggcttggtta aaaatgatta aaatacacta agcctttgaa atcaaaggag aatttaatga
     6961 tttacactaa tcagcttcgt tacagtggca aagatggcat ctatttccac ttaagagaca
     7021 atgtagatgc cttcttaacc ttatcagcaa ctgaaaacat ggaatttgat agccctatgc
     7081 aggtgactac acagaacatg caattatgaa ggaagcttat tattaactcg tcagggtcaa
     7141 ttagtagaaa acttcattga cactctggaa aactggcgtg accagaagca gattatttct
     7201 gttatctgta aagatggaat taaaattgac gattccatta ttaccaactt taaagcctct
     7261 aaagatgtta ctatctctaa cggtcttcgt atccagctaa cttttcagga aattaacttt
     7321 aaagcaatcg taggtcaaac tgatatttca gcagccactg gcaaaacggc taccacaaat
     7381 gacggtggtg tgaccagtaa gaagaatgca gg
//
LOCUS       DAJVXP010000175         7253 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVXP010000175 DAJVXP010000000
VERSION     DAJVXP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.175"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..273
                     /locus_tag="OQS10_003723"
     CDS             <1..273
                     /locus_tag="OQS10_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS2294647.1"
                     /translation="ATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQIRR
                     ISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            274..1890
                     /locus_tag="OQS10_003724"
     CDS             274..1890
                     /locus_tag="OQS10_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS2294648.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(1946..2098)
                     /locus_tag="OQS10_003725"
                     /pseudo
     CDS             complement(1946..2098)
                     /locus_tag="OQS10_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2121..2498)
                     /locus_tag="OQS10_003726"
     CDS             complement(2121..2498)
                     /locus_tag="OQS10_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294649.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(2908..3279)
                     /locus_tag="OQS10_003727"
     CDS             complement(2908..3279)
                     /locus_tag="OQS10_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294650.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(3340..3837)
                     /locus_tag="OQS10_003728"
     CDS             complement(3340..3837)
                     /locus_tag="OQS10_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294651.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(3906..4430)
                     /gene="sat2"
                     /locus_tag="OQS10_003729"
     CDS             complement(3906..4430)
                     /gene="sat2"
                     /locus_tag="OQS10_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS2294652.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(4525..4998)
                     /locus_tag="OQS10_003730"
     CDS             complement(4525..4998)
                     /locus_tag="OQS10_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS2294653.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            5330..5866
                     /locus_tag="OQS10_003731"
     CDS             5330..5866
                     /locus_tag="OQS10_003731"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS2294654.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            5981..6307
                     /locus_tag="OQS10_003732"
     CDS             5981..6307
                     /locus_tag="OQS10_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2294655.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(6495..6734)
                     /locus_tag="OQS10_003733"
     CDS             complement(6495..6734)
                     /locus_tag="OQS10_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS2294656.1"
                     /translation="MTPFRYNSDLTSGSLQTREYRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1996 a         1551 c         1672 g         2034 t
ORIGIN      
        1 gcgacatcga gctggctgtt aaagcaaact cctaacggaa cctctcttgc aaaaaatcta
       61 cagaaactgc ctttggtagc gctttgctta aagcgttact cagagagtgt ggaagattat
      121 caaattagac ggattagcca agcttttatt aagcttaaac aggaagatgt tgagcttagg
      181 cgctggcgat tattaagaag tgcaacgtta tctaaagagc ggataactga ggaagcacaa
      241 agattcttgg aaatggttta tggggaagag tgagtggtta ggctagctac atttaatgac
      301 aatgtgcagg ttgtacatat tggtcattta ttccgtaact cgggtcataa ggagtggcgt
      361 atttttgttt ggtttaatcc aatgcaagaa cggaaatgga ctcgatttac tcatttgcct
      421 ttattaagtc gagctaaggt ggttaacagt acaacaaagc aaataaataa ggcggatcgt
      481 gtgattgagt ttgaagcatc ggatcttcaa cgagccaaaa taatcgattt tcctaatctc
      541 tcgtcctttg cttccgtacg caacaaggat ggagcgcaga gttcatttat ttacgaagct
      601 gaaacaccat atagcaagac tcgttatcac atcccacagt tagagctagc tcggtcatta
      661 tttttaatta actcctattt ctgtcgaagc tgtttgagca gtaccgcttt acagcaagag
      721 ttcgacgttc agtatgaggt tgagcgagat catttagaga taaggatctt acccagttca
      781 tcgtttccta aaggggcgtt agagcagtcg gccgtagtgc agcttttggt ttggttgttt
      841 tcggatcaag atgttatgga ttcgtatgaa agtattttta ggcactatca acaaaataga
      901 gaaattaaga acggcgttga aagctggtgc tttagctttg accctccgcc catgcagggt
      961 tggaaattac atgtaaaagg acgttcttct aacgaggata aggattattt agttgaggaa
     1021 atagtaggtt tagaaatcaa cgctatgctt cctagcacaa cagctattag ccatgcctct
     1081 tttcaggaaa aggaggcagg tgatggtagt acgcagcaca tagcggtttc aacagagtca
     1141 gttgttgatg atgagcatct acagttggac gatgaggaaa cagccaatat agacacagac
     1201 acacgagtca tagaggctga gccgacatgg ataagtttta gtagacctag tcgaattgaa
     1261 aaatctcgca gggcaagaaa aagtagccaa actattttag aaaaagaaga agcaacaaca
     1321 agtgaaaata gtaatttggt tagtactgat gagccacact taggtggtgt cctagcagcg
     1381 gcagatgtgg gtgggaagca ggatgcaacc aattacaact ctatttttgc taatcgattt
     1441 gctgcttttg atgagctact ttcaattcta aaaactaaat ttgcatgtcg ggtgcttttt
     1501 gaagaaacct tggttttgcc aaaagttggg cgtagccgat tacatctgtg taaagatggc
     1561 tcaccaagag tgattaaagc cgttggggtg caacgtaatg gcagtgaatt tgtattgcta
     1621 gaggtggatg catcggatgg ggtgaaaatg ctttctacca aagtgttgag tggcgttgat
     1681 agcgaaacat ggcggaatga ttttgaaaag atacggcgtg gagtggtgaa gagctcattg
     1741 aattggccaa atagtttgtt tgatcaatta tatggacaag acgggcatag aggggtgaat
     1801 catccaaagg ggttggggga gctgcaagta tcgagagagg atatggaagg gtgggctgag
     1861 agagtggtta gagagcaatt tacgcattaa aggaatgact gaaagagcct gtaaaccctt
     1921 ttgtgtaagt gcttttgccg gtcagttaaa ggtggccatt taaacggtca ccaaattcga
     1981 tcataaaacg gttcatggcc ggcttccagt tgcggatcgg catcgtccat ttcttggtcg
     2041 ccgcctggat agccaggtac accaccttca tcgctgattc gtccgtaggg aacaccttga
     2101 gttcatatta atggaatttt ctacaaatag cctccgtggt tttgaggggg gattacagac
     2161 gatccatagt agtaatccaa tgagttcttg agcgcggcga catgtttgga cgccttggca
     2221 aaaattagag cctgcttgaa gtgcaggcga gcacgtgctt ggacgattga tccattcgcg
     2281 gtcaaaaact caatcttgga tgacagcgtg tcaggaaatc caatatcgaa gtcccgcccc
     2341 gtgtcaaagt agggcatttc atgatcaaag gacgaagctt cccaggccac ttccctaata
     2401 ttccagccgc tagaaacaag atttcgtacg acttcatcaa cttttttgct ttccaacttg
     2461 tcacaaagtg cataggcaag agctttggcg gcagacacgt gcgtttcctc ttaaacatag
     2521 gtcgaacgga tacccgaggc aaagcggagc attagtccca aagcatctcc ggtagatccg
     2581 gaggctccat actgaaggca tggcatcttg ggcattgata tttgaaactt ctctctagat
     2641 agcagggatt tcttggcctt gcctcatgct gatagcttgt aaatatcaca cgatccttag
     2701 ccccacaaag tgagcatact ccgtagcttt catttataga aatcgtcgtt agattgtcgc
     2761 agcatgtgca cgttccgact atttgctcag gaagcatcgc cataccttgg cctaggtaaa
     2821 gaagatcaga ctcgtagcca catcggtggc aactgagctt gtacgcaatg cccatctggt
     2881 tgtctcacct gaatcgtaac gtcagagcta ttcatccatc cattcggcat caatgtatgc
     2941 cactaagccg accagctgac cgtttggact gaagtatgtt gcctgcaccc cacttgacat
     3001 ctcatcaata ccaccgcact gcgctaggcg ccggtcccac ttgcccacag aagcgtcagg
     3061 atcggctgag ttccccaaga cccttgtgag ccatgcctcc tttaactcaa cctctcgaat
     3121 gattgcgtcg gccagctcct ccgcgttatc tagttcgaca tagtctggaa ttcggacgta
     3181 cgttctgaac acagctggat acttacttgg gcgattgtca tacatgacat caacaatgta
     3241 cccgtttgtg taaccgtctc ttggaggttc gtatgacact agtggttccc ctcagcttgc
     3301 gactagatgt tgaggcctaa cattttatta gagagcaggc tagttgctta gatacatgat
     3361 cttcaggccg ttatctgtca gggcaagcga aaattggcca tttatgacga ccaatgcccc
     3421 gcagaagctc ccatctttgc cgccatagac gccgcgcccc ccttttgggg tgtagaacat
     3481 ccttttgcca gatgtggaaa agaagttcgt tgtcccattg ttggcaatga cgtagtagcc
     3541 ggcgaaagtg cgagacccat ttgcgctata tataagccta cgatttccgt tgcgactatt
     3601 gtcgtaattg gatgaactat tatcgtagtt gctctcagag ttgtcgtaat ttgatggact
     3661 attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga gaaatgtcgt agttggatgg
     3721 ggagtagtca tagggaagac gagcttcatc cactaaaaca attggcaggt cagcaagtgc
     3781 ctgccccgat gccatcgcaa gtacgaggct tagaaccacc ttcaacagat cgcgcatagt
     3841 cttccccagc tctctaactc ctgaattaag ccgcgccgcg aagcggtgtc ggcttgaatg
     3901 aattgttagg cgtcatcctg tgctcccgag aaccagtacc agtacatcgc tgtttcgttc
     3961 gagacttgag gtctagtttt atacgtgaac aggtcaatgc cgccgagagt aaagccacat
     4021 tttgcgtaca aattgcaggc aggtacattg ttcgtttgtg tctctaatcg tatgccaagg
     4081 agctgtctgc ttagtgccca ctttttcgca aattcgatga gactgtgcgc gactcctttg
     4141 cctcggtgcg tgtgcgacac aacaatgtgt tcgatagagg ctagatcgtt ccatgttgag
     4201 ttgagttcaa tcttcccgac aagctcttgg tcgatgaatg cgccatagca agcagagtct
     4261 tcatcagagt catcatccga gatgtaatcc ttccggtagg ggctcacact tctggtagat
     4321 agttcaaagc cttggtcgga taggtgcaca tcgaacactt cacgaacaat gaaatggttc
     4381 tcagcatcca atgtttccgc cacctgctca gggatcaccg aaatcttcat atgacgccta
     4441 acgcctggca cagcggatcg caaacctggc gcggcttttg gtacaaaagg cgtgacaggt
     4501 ttgcgaatcc gttgctgcca cttgttaacc cttttgccag atttggtaac tataatttat
     4561 gttagaggcg aagtcttggg taaaaactgg cctaaaattg ctggggattt caggaaagta
     4621 aacatcacct tccggctcga tgtctattgt agatatatgt agtgtatcta cttgatcgat
     4681 caggcttttg tatatctccc caccacctga aacaatgaca tgatccgtta ttttctttag
     4741 gttggttaaa gcatctttaa ttgatggaaa gatcaatacg ttctcattgt cagatgtaaa
     4801 acttgaacgt gttacgaccg catactttcg gttgggtaat gctcccattg attcaaaagt
     4861 cttgcgtcca accaacagcc attggttata ggtaatagct ttaaacagga gctgttcacc
     4921 tttggcactc catggaatat cagggccatt cccgataact ccattcttcg atatagctac
     4981 cattagtgat agtttcacaa ttcttcctca gaggttaaca ttttattagt aagcatgctc
     5041 gttttgtcac ccgctgatgc ttaccgttaa ttaatataat cacttgtatt aaatagactt
     5101 tctttaaaaa tacaagacac tctgttatta caaatcgtgc atgccgtcta tcctgcttgc
     5161 acgatgcaca ttatctcacc caaagaactc ataacatttt aatttattta gcattaaatt
     5221 aaaactctac cggtaaacaa gcatctctag gcgtaaaaaa caggcacttt tttaaatccc
     5281 agctagaata ggctgtatag gcagacagtt gcaagacaag gagcgtttta tgtctaacag
     5341 tccattttta aattctatac gcacggatat gcgacaaaaa ggttatgcgc tgaaaactga
     5401 aaaaacttac ctgcactgga ttaagcgttt tattctgttt cacaaaaaac gtcatcctca
     5461 gaccatgggc agtgaagagg tcaggctgtt tttatccagc ttagcaaaca gcagacatgt
     5521 agccataaac acgcagaaaa tcgctttaaa tgccctagct tttttgtaca acaggttttt
     5581 acaacagccg ttgggcgata ttgattatat ccctgcaagc aagcctagac ggctaccctc
     5641 tgttatctct gcaaatgaag tgcaacgcat tttgcaggtt atggatactc gcaaccaagt
     5701 tatttttacg ctgctgtatg gtgcaggttt gcgcattaat gaatgcttgc gtttgcgggt
     5761 taaagatttt gattttgata atggctgcat cactgtgcat gacggtaagg gtgggaaaag
     5821 cagaaacagc ctactgccca cgcgcctaat cccagcaata aaataactca ttgagcaagc
     5881 gcggcttatt cagcaagacg acaacttaca aggcgtaggg ccatcgctgc cttttgcttt
     5941 agatcacaaa tacccttctg cttatcgaca agcggcgtgg atgtttgtct ttccctccag
     6001 cacgctctgc aaccacccgt ataacggcaa attatgccgc catcatctgc atgactccgt
     6061 tgcgcgaaag gcattgaagg cagccgtaca aaaagcaggc atcgttagca agcgtgtcac
     6121 ttgtcataca tttcgtcact cgtttgctac gcatctatta caagcggggc gtgatattcg
     6181 cactgtgcaa gaactcttag ggcataacga tgttaagacc acgcaaatct atacgcatgt
     6241 gttgggtcag cattttgccg gcaccaccag tcctgcggat ggactgatgc tacttatcaa
     6301 tcagtaaaag tagataaata tctttatgta gccctgagta gcccctgata ctttttatgg
     6361 tgaaaaagat aaacaatcat agaggggata aatatgagcc agttctacca ttgttcagat
     6421 aagtttaagc aagatactgt caatcacagt gaccattcat ggtcattaca cttcctccag
     6481 ttgccagaca atgcctaaca taaaaacgca aaagcccaaa agtcagagct taggtgttct
     6541 aggcgttttc taagcgctga agaaatacgt cttaccgtag attgcgtgcg tttctgaagc
     6601 acattttcct tatacatggc tttatcccag gcagcttcat caagctcttg gagtaacaag
     6661 ccagtaataa tgcgatactc tcgggtttgt agcgagccgc tggtgagatc ggagttatag
     6721 cggaaagggg tcatttgtaa ttatttgtgg gtggaggata gttaacttat tattgtagtt
     6781 ggaagaggct gctttccaaa cattagcacc tcgtaatgct tgttactgta tattaattta
     6841 tgctcttagc gcatgttaat gggctattac aatgcataat gtccacatca agataactgt
     6901 ggcttaacac ctgtacaagc gaaaaaaatc aatcacctgt ccggaaatac gggatcactg
     6961 taaagtgttg gtgaggcagt ctaggagggg gatttaattt gggatactga tgaaaggatt
     7021 atgatcagtt ggatatagga tcgtactaag ctgagcggca ggttagctag attagttaac
     7081 cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac
     7141 gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc
     7201 catttccacc cctcccagtt cccaactatt ttgtccgccc acagggctgc cga
//
LOCUS       DAJVXP010000176         7178 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVXP010000176 DAJVXP010000000
VERSION     DAJVXP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.176"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            45..1505
                     /locus_tag="OQS10_003734"
     CDS             45..1505
                     /locus_tag="OQS10_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS2294657.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="OQS10_003735"
     CDS             complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="OQS10_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS2294658.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1921..2607)
                     /gene="ydfH"
                     /locus_tag="OQS10_003736"
     CDS             complement(1921..2607)
                     /gene="ydfH"
                     /locus_tag="OQS10_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS2294659.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="OQS10_003737"
     CDS             complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="OQS10_003737"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS2294660.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3579..5624
                     /gene="dcp"
                     /locus_tag="OQS10_003738"
     CDS             3579..5624
                     /gene="dcp"
                     /locus_tag="OQS10_003738"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS2294661.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5669..6187)
                     /locus_tag="OQS10_003739"
     CDS             complement(5669..6187)
                     /locus_tag="OQS10_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS2294662.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            6463..6855
                     /gene="ydeI"
                     /locus_tag="OQS10_003740"
     CDS             6463..6855
                     /gene="ydeI"
                     /locus_tag="OQS10_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCS2294663.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1842 a         1662 c         1796 g         1878 t
ORIGIN      
        1 taacaaaaca tatagtttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggcttag
      361 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
      421 aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg
      481 ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc
      541 tggcccgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
      601 cagaaaacgg tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa
      661 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
      721 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgggcgatc
      781 ctgcgggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg
      841 gacgtccaga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
      901 agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg
      961 ctggatatca gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg
     1021 gcttgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     1081 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     1141 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     1201 tggcgcatga cagcaagttc gatctgctgg cactgggcgt cgcgggttgg atgcgttatg
     1261 tcggtggtgt tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta
     1321 ttcagaaggc tgtacaaagt agtgctgaag ggaaagcgcg cgtccagtca ttgctggcga
     1381 ttaaggcgat ctttggtgat gatttgccag acaatagttt gtttactgca aaagtgacgg
     1441 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     1501 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     1561 tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     1621 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     1681 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     1741 tcataggtat tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     1801 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgataca
     1861 tgcatgagcc ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa
     1921 ttactcttcg ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat
     1981 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2041 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt ggtcgaaact
     2101 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2161 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     2221 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     2281 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     2341 gataaaactg ccattgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     2401 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     2461 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     2521 caggcaatgg acaatgtcgc gacgaagaat acgataaatt tgctgattga caggctgtgt
     2581 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     2641 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     2701 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     2761 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     2821 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     2881 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     2941 taccatgcag gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3001 cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat
     3061 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt
     3121 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3181 tgcccaacgc caggccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa
     3241 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     3301 gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     3361 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     3421 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     3481 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     3541 gcctacgcta tggaacatct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     3601 ttgtgcaaag cacactaccg tatctggctc cccattttga tcaaattgcc aatcatcact
     3661 atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc
     3721 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     3781 tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     3841 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     3901 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     3961 ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc cttgccggag
     4021 ccaaacttgc gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4081 tgaccagcca gtttaaccag cgattactgg cagcaaataa atccggcggt ctggttgtga
     4141 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4201 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     4261 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     4321 cgcgagcgga aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga
     4381 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     4441 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     4501 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     4561 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     4621 aatttgatct tgatgaagcg cagctcaagc catattttga attaaacacg gtgttgaatg
     4681 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     4741 ttcctgtcta ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc
     4801 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     4861 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     4921 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     4981 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5041 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5101 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5161 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     5221 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     5281 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     5341 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     5401 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     5461 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     5521 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgc ggtaaggctc
     5581 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     5641 gcctggggat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     5701 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     5761 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     5821 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     5881 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     5941 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6001 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6061 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6121 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6181 tgttcactgt tttttgtcca cgctattata tgtaaaggtg atttgctttt tctgtatgct
     6241 cgtccaatat aataattttc aaacatataa atccgattat tctgaaaaca tgtttaagac
     6301 tcttcaatct tcttaacttt agtgagtcaa attctctgaa tatttgacag ctagtgggct
     6361 gattttaaat gccttataac aattggcagt attcttattt ggcaattata aaaatagcaa
     6421 ccagacttaa taagtcaaac caaacgcaat taaggagtta gtatgaaatt tcagtccatt
     6481 gtattagcaa gttttcttgt catgccttat gcgttggcag atgaccaggg cggtttaaaa
     6541 caggatgcag cgccaccgcc gcctcatgca atagaagatg gctatcgcgg aaccgatgat
     6601 gcaaaaaaaa tgaccgttga tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta
     6661 cgcggtaatt tgatttctca caaaggagag gaccgttacg tttttcgcga taagagcggt
     6721 gaaattaatg ttgttattcc tgcggccgtc tttgatggac gagaagttca gccggaccag
     6781 atgatcaaca ttagcggcag tctggataag aaaagcgcgc cggccgtcgt tcgggtcact
     6841 catttgcaga aataaaaata aattagcctg atggcctgaa ctactgggcc attagggtat
     6901 tgggttatgc tatttgtcat ccttttattt acgataatgc agaggtgatt tacagaatta
     6961 aacacttttt tatccgctga ttttgtttta aatcacaatg ttatctcaga gatgactcat
     7021 aacttcggat ttctatgaaa atattgacgt aattttgctt ttctctcgtt agaatagcgc
     7081 gcacaaggaa ctgtgaaaaa ggagtggcaa tgatcaagaa gacaacggaa attgatgcca
     7141 tcttgttaaa tctcaataag gctatcgatg cccactac
//
LOCUS       DAJVXP010000177         7124 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVXP010000177 DAJVXP010000000
VERSION     DAJVXP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.177"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            7..438
                     /locus_tag="OQS10_003741"
     CDS             7..438
                     /locus_tag="OQS10_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS2294664.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            521..1423
                     /locus_tag="OQS10_003742"
     CDS             521..1423
                     /locus_tag="OQS10_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS2294665.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1478..3640)
                     /gene="uvrD"
                     /locus_tag="OQS10_003743"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1478..3640)
                     /gene="uvrD"
                     /locus_tag="OQS10_003743"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS2294666.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3724..4440)
                     /gene="yigB"
                     /locus_tag="OQS10_003744"
     CDS             complement(3724..4440)
                     /gene="yigB"
                     /locus_tag="OQS10_003744"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS2294667.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4440..5336)
                     /gene="xerC"
                     /locus_tag="OQS10_003745"
     CDS             complement(4440..5336)
                     /gene="xerC"
                     /locus_tag="OQS10_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS2294668.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5333..6040)
                     /locus_tag="OQS10_003746"
     CDS             complement(5333..6040)
                     /locus_tag="OQS10_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS2294669.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6037..6861)
                     /gene="dapF"
                     /locus_tag="OQS10_003747"
     CDS             complement(6037..6861)
                     /gene="dapF"
                     /locus_tag="OQS10_003747"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS2294670.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6898..7101)
                     /locus_tag="OQS10_003748"
     CDS             complement(6898..7101)
                     /locus_tag="OQS10_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2294671.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1641 a         1992 c         1854 g         1637 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcgac atcgttcgtc tggatgcctt caaaaccagt
      121 aaagagattc agtttgcggt gcagtcaggg ccaatgttga tggaaaacag tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttgatggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagagga aaggttaagc attgtacact cgaggaggcc agacaaactg gggctggcta
      481 cacagctgaa aaatcatcag ataaatgaag ggaaacaaaa atgaaaaaaa cgctcgcaac
      541 gttattttta ttaacgtgtc tgggatcatc atccgcttat gcggataacg ctttgatttt
      601 acaaactgat tttagcctga aagatggtgc cgtctcggcg atgaaagggg tcgcattcgg
      661 tgttgaccat aatcttaaaa tatttgattt aacacacgaa attccaccgt ataacatctg
      721 ggaaggcgct taccgcctgt atcagaccgc cagttactgg ccgcaaggtt cggtatttgt
      781 cagtgtggtc gatcctggtg ttggtacaga ccgtaaatcg gtagtgctaa aaaccaaaaa
      841 cggtcaatac ttcgtttcac cagataacgg tacgctcact ttggtggcgg aatcgctggg
      901 tattgagagc gtccggcaaa tcgatgaaaa aaccaaccgc ctgaaaggtt ccgaaaagtc
      961 atataccttc cacggtcgtg acgtgtatgc ctacaccggt gcacgcctgg cttccggagc
     1021 gatcacgttc gaacaggtgg ggccagagct tcctgcaaaa gttgtcgaac tttcttatca
     1081 gaaagccaaa gcaacaaaag gtgaagtgaa gggcaatatc ccgattctcg atattcagta
     1141 cggcaacgtc tggagcaata tcagcgacga gttgctgaat caggcaggaa tcaaactcaa
     1201 cgacacgttg tgtgtaacca tttccgaagg ttcccagcaa aaatacgcag ggaaaatgcc
     1261 gtatgtcgca agcttcggcg atgtgccgga aggccagccg atggtgtatc taaacagctt
     1321 attgaatgtt tccgtggcgc tcaatatgga taatttcgcg cagaaacatc aagttgcgtc
     1381 tggtgctgac tggaatattg atgtgaagaa gtgcgctaag taaggtgctg atgcaggatg
     1441 cggtgtggcg caccgcatcc tgcaacggag ttgtaaatta caccgtctcc agccgggcgt
     1501 aagccgccac cagccatttg attccctggc cctggaatgc cacctgcaaa cggctatgtt
     1561 caccgctgcc ttccatattg acgatggtgc cttcaccaaa cttagcgtgg cgcacgcgct
     1621 ggccgagttt gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctaa
     1681 ccgggcggct taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcagctcgc
     1741 cgataaagcg cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt
     1801 aggtcagcgt cagtttctgc atcgcacggg ttacgccaac gtaggccaga cggcgttctt
     1861 cttccagacg tccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac
     1921 cgacgataaa cacctgcggg aactccagcc ctttcgccga gtgtagcgtc atcaactgca
     1981 ccgcatcctg ccaagtatcc gcctgccctt cacctgcttc cagtgccgca tgggagagga
     2041 acgcctgcag cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg
     2101 tcaccagttc ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt
     2161 acatggtacg caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat
     2221 cggcagtttc ttgcgctaag gcatcgatta attccataaa tcgctgcaag gcgctggcag
     2281 ctcgcccggc gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact
     2341 ggcgatcgcg cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg
     2401 gcgtattcac cacgcgctca aacgccgcgt cgtcgttacg gttggcaatc agacgcagat
     2461 acgagagcgc gtctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt
     2521 acggcatact ggcctgcaac agtgcttctt ccagtacacg cgactgggcg ttactgcggt
     2581 agagaatggc gcactcggca agcgccccgc cgttgtcctg ccaagttttg atgcggttaa
     2641 ccacaaaacg cgcttcgtcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt
     2701 ccgcgccatc ggtccacagt tttttaccca gacgcccgtt gttgttttca atcagggcgt
     2761 tcgcggcgct cagaatattg ctggtagagc ggtagttctg ctccagacgg atagtttcag
     2821 caccggggaa atcattgagg aaacgctgaa tattctctac ctgcgccccg cgccagccgt
     2881 agattgactg gtcgtcatcg ccaacgatca tcactttgcc ggtatcgccc gccagcaggc
     2941 ggatccacgc gtactgaatg ttgttggtat cctggaattc gtccaccagg atgttggtaa
     3001 aacgttcgcg gtagtgttgc aggatatgcg gcttgttaag ccacaactcg tgagcgcgca
     3061 gcagcagctc ggcgaagtcc accaggcccg cacgatcgca cgcttcctga tacgcctgat
     3121 acaccttctg ccaggtctgc tccaccggat taccgtagct ttgaatatga tgcggacgca
     3181 ggccttcatc tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct
     3241 cgtcgaggtt catggctttg atcagacgct taagcaggcg tagctggtct tcactgtcga
     3301 ggatctggaa atcctgcggt agattggcgt ccatatggtg cgcacgcagc aaacggtgcg
     3361 ccagcccgtg gaaggtgccg acccacatgc cgccctggct ggtgcccatc agttgcccga
     3421 tacgatgacg catctccgcc gccgctttgt tggtaaacgt cacagccata atcgagtatg
     3481 gcgagcagtt ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac
     3541 cactgcccgc gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgct
     3601 gtttgtcatt aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat
     3661 aggttgggcg gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttac
     3721 tgattatatc agcgaggtca gagatgccaa ccgggaaatt tccagatgcg gcagtaaacg
     3781 gctgtcccag gtttgcatca gatcgccatt ttccggtctg atccaacaag cctgcattcc
     3841 gctgcgaatt gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc
     3901 gatcggcacg ttgagttttt ccgcagccaa aaagtacata tcgctgaacg gttttgagcg
     3961 cccatgcggg ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg
     4021 ctgggcgtta ccgttggtga tcgccaccag cggccatttc ttcgccagct gttttaaggt
     4081 gtcgtgagtt tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt taatcattgc
     4141 tgcgtgtgcg cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc
     4201 aatcgaacga aaacgccaac gcgtcacgtc gtgataaatc tcgggttccg cttcccgtac
     4261 cgcctggcgc aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt
     4321 ttgcacaaag gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag
     4381 ggtatcatcc aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat
     4441 tatttccccc gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa
     4501 tcaagatgag tatagatttg cgtggtggag aggttggcat gacccagcag ctcctgcaca
     4561 ccacgaagat cgccgctcga ctccagcata tgcgtggcga acgagtgacg taatttatgc
     4621 ggatgaacgt gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca
     4681 ttacgcgcgg agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca
     4741 aacaggtcgc gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc
     4801 aggcggcgct ctttgctgcc tttccccatt acccacactt caccagactc caggtcgagg
     4861 tgtttgatat ccagccccac cagctcggaa agacgcagac ccgcgccgta catcacttcc
     4921 agcattgcac ggtcgcgtac agcgagggga tcgttgatat caatgtccag cagccgattc
     4981 atatcgtcga cgtcgatatt tttcggcaga tgacgcggcg ctttcggtgc cgaaacacct
     5041 ttcgccgggt tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc
     5101 gcagaaagcc gtaacgcgag gcttgctgct cccagccctt tacggcgact gcgtacagca
     5161 aaattgcgca ccatcgtcac atcacattgc tgccagcttt gcaggccgtt ttcgctggca
     5221 aaattgatga tcgcctcaag ctgacgctgg tagttaagca gggttatcgg gctaagctgg
     5281 cgctccacgc tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc
     5341 gttcaatcca acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg
     5401 ttccctgccc ttgttgatag tgactggcat cgcgactggt aaacagcacg acacccaaat
     5461 cagcatcgct tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca
     5521 ccaccagcag ctctggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct
     5581 gaatacgcag cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg
     5641 cacctaagcg ccagcgatcc ggaaacaggc gcagactcgc acctgccagg ccgagatcgc
     5701 gcgcccagcg gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc
     5761 gctgcaggta gagtaggcga taaaacaggc cttcgttggc gatagcctgt tccatcaaca
     5821 gcgccatgtt ctcttccaga acatgaatat gattacgtgc gcgggccatg tgccactcga
     5881 ccaacgaaac ggtgccgcgt accggatgcg gcacacgtat cgcttctact gcgcgcgcat
     5941 tacggataaa aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct
     6001 ccgtgagtgt ttcctgcagt tcttcccctg gttgcttcat agatgaataa atccgtcgta
     6061 gacatgtacc gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc
     6121 aagacgaccg ccggggagtt ccacgcgtac ttcttcggcc agcaaacctt gctgaatccc
     6181 tactgcaacc gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc
     6241 ataaacgcgt aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg
     6301 ctccggaaaa cgctcgtggc tttccagaac aggaccaagc gtttctaccg ccgcggtatc
     6361 gacatcatcg acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat
     6421 tgtctgctcg gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac
     6481 ggcggaaggt tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac
     6541 ggtcagaacc atccgcccgt tggcggtgct gacgcggata tcacgcttat tggtcagtcc
     6601 tttcagacgc acaaaacggg caaagcagcg cgcaccgttg ccgcactgcg ccacttcact
     6661 gccatcagca ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac
     6721 caccagcagt tggtcaaacc ctacccccag gtgccgatca gccaggcgac gaatcagctc
     6781 cggtgaaaaa aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc
     6841 atgcattttc gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta
     6901 gtaattcacc tgggatggac catcgccagt ggcgcgatcg tttttatccg gcaccgtgga
     6961 ttgcgtttgc gtctctaccg gtttggtcgg cggcggtgcg tttttatctg caggcgggaa
     7021 atagagcgga cctttcagac cgcagcccgt caggctgaag agagtaagta atacagtgag
     7081 tgccttaaac acgtttttca ttatggattg cctgtaaagt tcat
//
LOCUS       DAJVXP010000178         7062 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVXP010000178 DAJVXP010000000
VERSION     DAJVXP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.178"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..302
                     /locus_tag="OQS10_003749"
     CDS             <1..302
                     /locus_tag="OQS10_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS2294672.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="OQS10_003750"
     CDS             complement(391..903)
                     /locus_tag="OQS10_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS2294673.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="OQS10_003751"
     CDS             1098..1862
                     /locus_tag="OQS10_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294674.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="OQS10_003752"
     CDS             1813..3216
                     /locus_tag="OQS10_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS2294675.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="OQS10_003753"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="OQS10_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="OQS10_003754"
     CDS             4573..5316
                     /locus_tag="OQS10_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS2294676.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="OQS10_003755"
     CDS             5320..6540
                     /locus_tag="OQS10_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS2294677.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..7050
                     /locus_tag="OQS10_003756"
     CDS             6544..7050
                     /locus_tag="OQS10_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294678.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1873 a         1684 c         1934 g         1571 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgtaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg
     6841 accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga tccggtatac
     6901 gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat gtccacgtca
     6961 gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg tgatgctgac
     7021 ggcaatgcag aaatttcctg gaagatttaa ggaacagacg aa
//
LOCUS       DAJVXP010000179         6861 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVXP010000179 DAJVXP010000000
VERSION     DAJVXP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.179"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..351)
                     /gene="rnpB"
                     /locus_tag="OQS10_003757"
     ncRNA           complement(<1..351)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQS10_003757"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(384..1529)
                     /gene="garK"
                     /locus_tag="OQS10_003758"
     CDS             complement(384..1529)
                     /gene="garK"
                     /locus_tag="OQS10_003758"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS2294679.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1626..2516)
                     /gene="garR"
                     /locus_tag="OQS10_003759"
     CDS             complement(1626..2516)
                     /gene="garR"
                     /locus_tag="OQS10_003759"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS2294680.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2546..3316)
                     /gene="garL"
                     /locus_tag="OQS10_003760"
     CDS             complement(2546..3316)
                     /gene="garL"
                     /locus_tag="OQS10_003760"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS2294681.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3332..4666)
                     /gene="garP"
                     /locus_tag="OQS10_003761"
     CDS             complement(3332..4666)
                     /gene="garP"
                     /locus_tag="OQS10_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS2294682.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5041..6612
                     /gene="garD"
                     /locus_tag="OQS10_003762"
     CDS             5041..6612
                     /gene="garD"
                     /locus_tag="OQS10_003762"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS2294683.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6761..>6861
                     /gene="prlF"
                     /locus_tag="OQS10_003763"
     CDS             6761..>6861
                     /gene="prlF"
                     /locus_tag="OQS10_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCS2294684.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREAL"
BASE COUNT         1682 a         1883 c         1688 g         1608 t
ORIGIN      
        1 tctgtcgtgg acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc
       61 cgggttcagt acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt
      121 accgtgccac ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta
      181 cctgatcccg cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt
      241 cccccccagg cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc
      301 cgtctccccc gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag
      361 tatagagggt ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg
      421 gcgatattac gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac
      481 gtacctatgc tggtcaatac gctgaagacc gcatcgatgc catgctgatg tacaacgcca
      541 acatcatcgg tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg
      601 tttgcaacgc caatcggtac cttgccgtga atactctggc tgtcaatacg cccttcaccg
      661 gtgatcacca gcgtacaatc gtgaatatgt tcttccagat tcagcgccgt agtgacgatt
      721 tcaataccac ttttcagttc cgcaccaaga aacgccatca gcgccgcgcc cataccgccc
      781 gcagctcctg cgccggggac atctttcaca tcaacatgca gcgctttttt aatgacatcg
      841 gcatagtgag agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt
      901 gggccaaaga tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg
      961 acgcgaatga cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga
     1021 gtattaagac taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc
     1081 cccagcgcct gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata
     1141 ataatgtttg ttgcactgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa
     1201 gtggttacga gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc
     1261 atttcaataa acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg
     1321 cccagcggcc ctgtaaccca ggcgtgacgt tcagccccct gggtggctgc aatcatcgct
     1381 tccaccgttc cttcaccacc gtcggcaacc ggaacagaaa catactgtgc atcaggaaaa
     1441 atttcccgaa atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta
     1501 taagagtctg gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg
     1561 gcgtttctct tattcggcct agattccagg cccggattgc aatacaccat ccgggcacga
     1621 cgtcattaac gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagggcgcta
     1681 tgatccgccg ttcctaaacc atctgcacgc agtgcctgca tcatctccat aaccgcagct
     1741 gtgagcagca gttgtgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta
     1801 atatgcagat caatgcggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg
     1861 gcatccagca cggtactgcc cgccagccca ccgcgaattg cctgataaac caggtccggg
     1921 ttaacgcccg ctttggttgc cagcgttaac gcttctgaca tcgcagcaat attcagcgcc
     1981 acaatgacct gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc
     2041 acggaacccg ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg
     2101 cccaccatca ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca
     2161 tccagcatat caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt
     2221 gcgatagaac tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc
     2281 tcacccagcg ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg
     2341 cactgttcag cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca
     2401 gcaatagctt ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt
     2461 ttactcattg gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca
     2521 accatctctc ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg
     2581 cagagcggaa gacgccaaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat
     2641 aacgacgcgc atcggcttcg accggcgcga gaataccgct gggtttgccg tgcgcgctgg
     2701 cacggttaaa aatatgctga attgcttttt gtacatccgg atgcgaggca ttgccgagat
     2761 ggcccaatgc cgcagccaga tcgctggggc cgacgaagat accgtctaca ccttcggtag
     2821 cggcaatagc atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtaa
     2881 tgttcttgtt cgactgagcg aaataatccg ccacggtgcc aaacatattg gtgcggtgag
     2941 aaacggagac gccgcgaatg ccttccggcg ggtaacgagt tgatgccacc gcctgctctg
     3001 cttcctcttt tgtttctaca aaaggaatca ggaagttata gaaaccgata tccagaagac
     3061 gcttaataat taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt
     3121 tcagggccat taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt tcgccatcca
     3181 gcaccagcca gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg
     3241 ctgaccagca accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga
     3301 aaacatcgtt attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt
     3361 cgccaactac gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca
     3421 gtgctgcatt gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa
     3481 tggaggcaac attgccaaag acgttaaaga cgccgccaca gaggccaaca atctctttcg
     3541 gcgcggtgtc agaaatcacc ggccagccca gagcaccaaa tcctttgcca aaaaaagcca
     3601 gcgccatcag catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg
     3661 aaaccagcaa cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc
     3721 gtttgatcag ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg
     3781 ccggaatcga ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat
     3841 aaatcgggaa ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa
     3901 atacgcccaa catcatgcgg ttagagagca attgcttgat gtaatgcagt ttgggaccgc
     3961 ttgctgccgc gctgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga
     4021 taaacttcag ctcttccgca gacatacgtg ggtgatctgt cgggttatga atcaacttga
     4081 tccacagcgc cgtcagcaca aaaccaatcc cccccataac ggtgaagacg tgctcccagc
     4141 cccaagcgaa agtcagccag ccaagcagcg gcgaaaagag cgccagcgag aaatattgcg
     4201 ccgagttaaa gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa
     4261 tacgggcgtt cgccgggaat gatggcgctt ccgagaaccc cagcataaag cgcataaaga
     4321 acatggagat ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga
     4381 acagcgacca gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca
     4441 accagccgcc ggggatttgc atcagcaagt aggcccagcc aaaagcagag aagatgtaac
     4501 ccatcgaaac cgcacttaac tgcaactctt ttgccacttc ggtaccagca atagacagcg
     4561 ttgcacgatc ggcgtagtta acggcggtaa caataaaaat aatcagtaat attaaatagc
     4621 tggtatgcac gcctttcttt tttacgtcaa cggtgtccag aatcatttta tttacctcgg
     4681 gtacttatgc tgatttttat tattatgggg aaggtgttat ttatgagttt tatttatgcc
     4741 gttacgacaa tgaactcggg aattagtata agcagcggga gaataataat cattgtgcaa
     4801 atgctaattt aatcagtact atttaaatat tattttgagc atatgcacat aaggttgcgc
     4861 gctaaagcac agatttgcgc tttaccttac cgggcggcac tgcaatccct gaaatgattg
     4921 acattgatca catttctgcg tttaaactcc tgacattctt atttcaccca atgaagtcat
     4981 ttatttttaa atgagaccag atcctcattt taataacccc tggctggaga atattgcaca
     5041 atggccaaca tcgaaatcag acaagaaacg ccaactgcgt tttatataaa agttcacgac
     5101 acagataatg tggcaattat tgttaatgat aatggcctga aagcaggaac gcgttttccg
     5161 gatgggctgg aattaattga acatactccc caggggcata aagtcgcatt gctggacatt
     5221 ccagctaatg gtgaaattat tcgttatggc gaagtgattg gttacgccgt gcgcgcaatc
     5281 ccacgcggaa gctggatcga cgaatcaatg gttgtactac cggaagcgcc gccgttacac
     5341 acgctgccac tggcaaccaa agtcccggcc cccttaccgc cgctggaagg atacaccttt
     5401 gagggctatc gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg catcaccacc
     5461 agcgtccact gtgtggcagg cgtggtggac tatgtagtaa aaatcattga acgcgatctg
     5521 ctaccgaaat acccgaacgt cgatggcgtg gtggggctga atcatttgta cggctgtggc
     5581 gtggcgatta acgcaccggc ggcagttgtg cctattcgta ccatccacaa tatttcgctg
     5641 aaccctaact ttggcggcga agtaatggtg attggtctgg gttgtgaaaa gttgcagcct
     5701 gagcgcctgc tggtcggcac ggatgatgtg caagctattc cagtagaaag cgccagcatt
     5761 gtcagtttgc aggatgaaaa gcatgtcggc tttcagtcca tggtcgagga tattttgcag
     5821 gtcgccgaac gccatctaca aaaactgaat caacggcagc gagaaacctg cccggcttca
     5881 gaactggtcg ttggtgtgca gtgcggtggc agcgatgcgt tttctggtgt aacggcaaac
     5941 ccggcggttg gctatgcgtc tgatctactg gtgcgctgcg gcgcaacggt gatgttttca
     6001 gaagtaacgg aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt gaacgaagag
     6061 gtcggcaaac ggctgctgga ggagatggag tggtacgata actatctcaa tatgggaaaa
     6121 accgaccgca gcgccaaccc ttcgccgggc aacaagaaag gcggtctggc aaatgtggtg
     6181 gagaaggcac tcggctccat tgctaaatcg ggtaaaagtg caattgttga agtgctgtcg
     6241 cccggtcaac gcccgactaa acgcggatta atttacgccg cgacgccagc cagcgatttt
     6301 gtctgtggca cgcaacaggt ggcttcgggt atcaccgtac aagtgtttac gaccggccgt
     6361 ggtacgccgt acggcctgat ggcggtaccc gtaattaaaa tggcaacccg caccgagctg
     6421 gcgaaccgct ggtttgattt aatggatatt aacgcgggca ctatcgctac cggcgaagag
     6481 agcattgaag aggtgggctg gaagttgttc cactttattc tcgacgtcgc cagcgggaag
     6541 aagaaaacct tctcggatca atgggggctg cataaccagc tggcggtgtt taacccggca
     6601 ccggtgacct gatttctttt ggaacgtccc tcgcaaaaca tggccttagt gccatgtttt
     6661 tattgtttaa agcccccacg tccattaata atgcatttgc attaccttag ttcaagctta
     6721 taatttgagc agaaaacagg ctgtaaaagg acaatgaatc atgcccgcta atgctcgctc
     6781 tcacgctgta ctgaccactg aatcaaaggt cacgatacgc ggacaaacaa ctatccccgc
     6841 gccagtgcgt gaggccttaa a
//
LOCUS       DAJVXP010000180         6859 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVXP010000180 DAJVXP010000000
VERSION     DAJVXP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.180"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..964
                     /locus_tag="OQS10_003764"
                     /pseudo
     CDS             1..964
                     /locus_tag="OQS10_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    32..148
                     /locus_tag="OQS10_003764"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1010..1360
                     /gene="traA"
                     /locus_tag="OQS10_003765"
                     /pseudo
     CDS             1010..1360
                     /gene="traA"
                     /locus_tag="OQS10_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            1375..1686
                     /gene="traL"
                     /locus_tag="OQS10_003766"
     CDS             1375..1686
                     /gene="traL"
                     /locus_tag="OQS10_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCS2294685.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1708..1890
                     /locus_tag="OQS10_003767"
                     /pseudo
     CDS             1708..1890
                     /locus_tag="OQS10_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1892..3142
                     /locus_tag="OQS10_003768"
                     /pseudo
     CDS             1892..3142
                     /locus_tag="OQS10_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            3139..3525
                     /gene="trbI"
                     /locus_tag="OQS10_003769"
     CDS             3139..3525
                     /gene="trbI"
                     /locus_tag="OQS10_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCS2294686.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            3522..3833
                     /locus_tag="OQS10_003770"
                     /pseudo
     CDS             3522..3833
                     /locus_tag="OQS10_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3828..4826
                     /locus_tag="OQS10_003771"
                     /pseudo
     CDS             3828..4826
                     /locus_tag="OQS10_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            4846..5593
                     /gene="traX"
                     /locus_tag="OQS10_003772"
                     /pseudo
     CDS             4846..5593
                     /gene="traX"
                     /locus_tag="OQS10_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            5648..6208
                     /locus_tag="OQS10_003773"
     CDS             5648..6208
                     /locus_tag="OQS10_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS2294687.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            6338..6550
                     /locus_tag="OQS10_003774"
     CDS             6338..6550
                     /locus_tag="OQS10_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS2294688.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1630 a         1664 c         2084 g         1481 t
ORIGIN      
        1 gccgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg gagtttgacc
       61 gcctctggaa aaaatgatgc cactgctgga taagctgcgt gagcagtacg gggtcggacc
      121 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
      181 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      241 gcgcgtatac gatgaaaatc acaaggtata cggtgtgcgt aaagtctggc gtcagttgtt
      301 acgggaaggt atcagagtgg ccagatgcac tgtggcacgt ctcatggcgg ttatgggact
      361 tgccggtgtt ctccggggta aaaaggtccg tacgaccatc agccggaaag ccgttgccgc
      421 aggcgaccgc gtaaaccgtc agttcgtggc agaacgacct gaccagctgt gggtggctga
      481 ttttacttac gtcagcacat ggcagggctt cgtctatgtg gcgttcatca ttgatgtgtt
      541 tgccggatac atcgtggggt ggcgggtctc atcgtccatg gaaacgacat tcgtgctgga
      601 tgcactggag caggcgttat gggcccgtcg accgtccggc acggtccatc acagtgataa
      661 aggttctcag tatgtatcgc tggcctacac acagcggctt aaggaagccg gattactggc
      721 atcaacagga agtacaggtg actcgtatga caacgcgatg gcagagatca tcaagggcct
      781 ttacaaagcg gaggtaatac accgtaagag ctggaaaaac cggacagaag tggagctggc
      841 cacactcacg tgggtggact ggtataacaa tcgacgattg ctggaaaggc tgggccatac
      901 tcctccggca gaagcagaaa aagcttatta tgcttccatc ggaaacgatg atctggcagc
      961 ctgagttcac agataaaaca ctctccagga aacccggggc ggttcagcgt taagtgttca
     1021 gggtacttct gcgcccgtca aaaagaaatc gtttttttct aaattcactc gtctgaatat
     1081 gcttcgcctg gttcgtgcag tgatcccggt tgctgttctg atgatgttgt tccctgagtt
     1141 agctatggct gctggtaaag gcacggacct gatggctaaa ggtcaggaaa cggttaaggc
     1201 gaccttcggt aaggactcca gtatcgttaa atgggttgtt ctggctgaag ttctggtcgg
     1261 tgcagtcatg tacatgatga ccaaaaacgt caagttcctg gtcggctttg ccatactctc
     1321 tgtatttatt gcagtaggta tgtctgttgc cggattctga caggaaataa aacgatgttg
     1381 ggagacgaga ataaacttaa gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc
     1441 tggtttggcc tgccactgga tgaactgatc cccgcagcaa tctgtgttgg ctggggtatc
     1501 acaacatcga aatatctgtt cggtattggt gcagcggttc tggtttattt cgggattaaa
     1561 aaactgaaaa aagggcgggg cagttcctgg ttacgtgacc tgatttactg gtatatgcca
     1621 acagccctgc tgcgcggtat ttttcataat gttcccgatt cgtgtttccg gcagtggatt
     1681 aaatagaact gataccagga ttgttatatg gaacacggtg cccgtttaag taccagtcgt
     1741 gtaatggcca tcgcctttat atttatgtca gtgcttattg ttctcagcct ctctgttaac
     1801 ctcattcagg gggtgaataa ctaccgtctt cagaatgagc aacgcactgc cgtgacccca
     1861 atgggattta atgtgccctt tgccgtgtca cacctaccgt aaccagctgg cgtttatcga
     1921 tattttcttc cggggaatga ataacaccaa ctacaacatg gcggtctgtg gcacctccgg
     1981 ggccggtaaa accgggctga tacagccact tatccgcagc gtgctggact ccgggggctt
     2041 tgccgtggtg ttcgacatgg gggatggcta caagtccctg tgtgagaaca tgggtggcgt
     2101 atatctggac ggtgaaaccc tgcgttttaa cccgtttgcg aacatcaccg atatcgacca
     2161 gtcagcggag cgtgtccgtg accagttgtc agtgatggcc agccccaacg gtaacctgga
     2221 tgaagtgcat gaaggtctgc tgctgcaggc ggtcagggcc tcctggctgg ccaaagagaa
     2281 cagggcacgt attgatgacg tggtggattt cctgaaaaac gccagtgaca gcgagcagta
     2341 tgccgggtca ccgactatcc gcagccgtct ggacgaaatg attgtgctgc ttgaccagta
     2401 cactgccaac ggcacttacg gccagtattt taactctgat gagccgtccc tgcgggatga
     2461 cgcaaaaatg gtggtgctgg agctgggcgg actggaagac cgtccgtcac tgctggttgc
     2521 ggtgatgttc tccctgatta tctacatcga gaacaggatg taccgcacgc cgcgtaacct
     2581 caagaaactg aacgtcattg atgaaggctg gcgtctgctg gacttcaaaa atcacaaggt
     2641 cggtgagttt attgagaaag gttaccgtac cgcccgtcgt cataccggtg cttatatcac
     2701 catcacacag aacatcgtcg actttgactc tgacaaggcc tccagtgcgg cccgtgcggc
     2761 atggggtaac tcctcctaca aaattatcct caaacagagt gcgaaggagt tcgcaaaata
     2821 caaccagctg tatccggacc agttccagcc actgcagcgc gacatgattg gtaagtttgg
     2881 tgcggccaaa gaccagtggt tcagttcctt cctgctgcag gtggaaaacc attcctcctg
     2941 gcaccgcctg tttgtggacc cgttaagctg cgccatgtac agctctgacg gcccggattt
     3001 tgagtttgtg cagcagaaac gtaaagaggg gctgagtatt catgaggcgg tgtggcagct
     3061 ggcgtggaag aagtcagggc cggagatggc ttcgctggaa gcctggctgg aagaacatga
     3121 gaaatacagg agtgttgcat gaccacaacg caaaaaacga ctgatgttac agccccccga
     3181 cgcagccact ggtggtggac ggtgccggga tgcctggcaa tggtgttact gaacgcagcc
     3241 gtcagttacg gcattgtcag actgaacgca ccgatgactg ccgccttcaa catgaaacag
     3301 actgtggatg cattttttga cagcgccagt cagaaacaac tgtcagaagc acagtcaaaa
     3361 gccctttcag cgcgttttaa cacggcactg gaagccagcc tgcaggcgtg gcagcagaaa
     3421 caccacgcgg tcattctggt gtcgcctgcc gtggtacagg gggctccgga tatcacccgg
     3481 gaaatccagc aggatattgc ccggcgaatg agagcggaac catgagatgc cgggggctga
     3541 ttgccctgct gatatggggg cagagtgtgg ccgccgccga tcttggcacc tggggtgatc
     3601 tgtggccggt aaaggagccg gacatgctga cggtgatcat gcagcgcctg acggcactgg
     3661 agcagtccgg tgagatgggc cggaaaatgg atgcctttaa ggagcgggtg atccgcaaca
     3721 gtctgcggcc ccctgccgtg cccggtatcg gacgcacgga gaaatacagc agccggttgt
     3781 ttgacccgtc cgtcagactg gctgcggata tccgggataa cgaagggcgg gtggccggct
     3841 ttgagtcagc ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata
     3901 accgtcaggg ctggacggat gccatcaaca atgccgtaca gaaaggaact gcccacgatg
     3961 tgtttgagcc gaaaccggac cgggaggtca tgaatgcaga gcggctgttc agtacggcgc
     4021 gggagctgcg ggacgtggcg gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg
     4081 gagacagtcc tgcacggttt attgctccgg gacgtaaata tccgcaaccg tatgtggcac
     4141 tgccggcgtt tgaccgtaac ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg
     4201 atgacggaaa cgggctgcgg ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg
     4261 cgcagttcgt ggccctgcag ggcagccgta acggagaaag cctgctggct gataatatgc
     4321 aggatggcgt ccggattgcc cgtgataatc ctgacagtgg tgtggtggta agcattgccg
     4381 gtgaaggccg tccgtggaat cccggtgcca tcaccgatgg ccgcgtgtgg ggggatatcc
     4441 cggacaacag cgtccagccg ggagccggaa atggcgaacc ggtcacggca gaggtactgg
     4501 cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg
     4561 tccgtaaaat ggcagagaac aaacctgacc tgccggacgg caaaacagag ctggctgtca
     4621 gggaaattgc cgggcaggag cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc
     4681 cggagagtgt gctgcgtgaa tcacaacggg agcgggaagc ggtccgggag gttgcccggg
     4741 aaaatctgct gcaggagcgt ctgcagcaga tggagcggga tatggttcgt gacctgcaga
     4801 aagagaaaac cccgggcgga gactgataca ggaagacaaa cactgatgac aacggataac
     4861 acgaacacga cacgtaacga ttcactggct gcacggaccg atacctggtt gcagtcattt
     4921 ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt gctgatggta
     4981 ctggaccaca tcaatctgat attccagctg aagcaggaat ggatgtttct ggccgggcga
     5041 ggagcctttc cgctttttgc cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc
     5101 cggcaaccgg ccattaaccg gctgtgggga tggggaatta ttgcccagtt cgcatattat
     5161 ctggcaggct ttccctggta tgaggggaat atcctgtttg cctttgcagt ggcggcacag
     5221 gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat tcttctgatg
     5281 gccctgttgg gggcctttgt ccggcaccag ttacggcatt gccgggctgc tgatgctggc
     5341 agtcagccac cgactgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg
     5401 cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg
     5461 tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat cattaccccg
     5521 tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg tgctgggcgt
     5581 tctggcgctg tgacgggtgt ggtatctttg accgtaacag gatgattcgt cagaggcaga
     5641 acacagcatg acagagcaga agcgaccggt actgacactg aagcggaaaa cggaagggga
     5701 gacgcctgtc cgcagccgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt
     5761 gaaaaagcag aagctggccg agaaagcagc ccgggaagca gagctggcgg caaaaaaagc
     5821 gcaggccaga caggcactgt ccatttatct gaacctgccc acgctggatg aggccgtgaa
     5881 taccctgaaa ccctggtggc cgggattatt tgacggtgac accccccggc ttctggcctg
     5941 cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa
     6001 aaaactgcgc agggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa
     6061 agccggtgcc tgccggtatg acacggaagg gtatgtgacg gagcatattt ctcaggagga
     6121 agaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaaccgga taaaggcaga
     6181 acttcaggcc gtgcttgatg agaaataaaa aaaagcctcc atcagagagg aggcagggaa
     6241 ataaggctta aaaggaaata acttccgctt ccgaaaggaa gccagtccac tgaattattt
     6301 atcacaataa tactgacaac taaaggggga caagaaaatg aatggattca gaaacagttc
     6361 acggaacggc caggtctggc gttaccagcg tgccggcggg cgggccgtta ttctggaagt
     6421 cagtggacgc tggatggaag cggcagaagc atggcgacgg gctgcctgtg ttgcgcccag
     6481 gacagactgg cagcagtttg cccgaaaaag ggctgaacac tgccaccggc gctgccgggg
     6541 aagggtgtaa cgaaaaaagc agccttcatt actccaggag tcagaaggct gaaaggagtt
     6601 tatgagattt gcttttttta atatccggtc gggcagtatg accggacagt tctgagtagc
     6661 acagagtttt ttccgtatta agtcgtgatt ttatattttg tgattaattt cacaaaataa
     6721 ggtgttattc agtgtgtgct gcaatattca ggatgcctga aatacagatg ctatttatct
     6781 gatatggaga ataacatgag gaaatagtaa ggcagtatga ctttccgtga gacagatgct
     6841 ggccaacgtg ggatattta
//
LOCUS       DAJVXP010000181         6538 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVXP010000181 DAJVXP010000000
VERSION     DAJVXP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.181"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OQS10_003775"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OQS10_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS2294689.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OQS10_003776"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OQS10_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS2294690.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OQS10_003777"
     CDS             2408..3157
                     /locus_tag="OQS10_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS2294691.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OQS10_003778"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OQS10_003778"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQS10_003779"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OQS10_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS2294692.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OQS10_003780"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OQS10_003780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS2294693.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="OQS10_003781"
     CDS             6018..6491
                     /locus_tag="OQS10_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCS2294694.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1651 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgttac
//
LOCUS       DAJVXP010000182         6532 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVXP010000182 DAJVXP010000000
VERSION     DAJVXP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.182"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..906)
                     /locus_tag="OQS10_003782"
     CDS             complement(<1..906)
                     /locus_tag="OQS10_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCS2294695.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="OQS10_003783"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="OQS10_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS2294696.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="OQS10_003784"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="OQS10_003784"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS2294697.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="OQS10_003785"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="OQS10_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS2294698.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OQS10_003786"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OQS10_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS2294699.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6532
                     /locus_tag="OQS10_003787"
                     /pseudo
     CDS             6387..6532
                     /locus_tag="OQS10_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga ca
//
LOCUS       DAJVXP010000183         6383 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVXP010000183 DAJVXP010000000
VERSION     DAJVXP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.183"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            6..326
                     /locus_tag="OQS10_003788"
     CDS             6..326
                     /locus_tag="OQS10_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCS2294700.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OQS10_003789"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OQS10_003789"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS2294701.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OQS10_003790"
     CDS             2213..2929
                     /locus_tag="OQS10_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2294702.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3382..3457)
                     /locus_tag="OQS10_003791"
     tRNA            complement(3382..3457)
                     /locus_tag="OQS10_003791"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Asn,seq:gtt)
     gene            complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OQS10_003792"
     CDS             complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OQS10_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS2294703.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5206..5281
                     /locus_tag="OQS10_003793"
     tRNA            5206..5281
                     /locus_tag="OQS10_003793"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5239..5241,aa:Asn,seq:gtt)
     gene            complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="OQS10_003794"
     CDS             complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="OQS10_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS2294704.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1814 a         1542 c         1372 g         1655 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     3421 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt
     3481 agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat
     3541 aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggttcg
     3601 gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc
     3661 cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg
     3721 atgcccagca cataaccgac gacaacccgg caaccccaca tgctcaacat agaaacccac
     3781 atggcgtaac gggcatcgcg agcaccttta aatccagcgg gtagcaccca tgaggcggac
     3841 caaataggca taaataaagc atttagccaa atcagaatca caacgacatg tttaacctgt
     3901 ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg
     3961 gccgttaatc caagagtgga aagccagaac acatgccgca actgaatctc tgcttgcgct
     4021 atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagagcc gagcgcactt
     4081 ccgggtaagt tgataagagc cgcaattgaa aacgcgataa aatttccggc aataacactg
     4141 gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat
     4201 tcgacactcg cgggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt
     4261 ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac
     4321 aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccagccctgc cccgacaaat
     4381 cccagtcccg gccaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga
     4441 atattcaggc taccgttaat caatagcggt attttcgtat tccctgcacc acgaagtgcc
     4501 ccgctaccaa taagagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa
     4561 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
     4621 tcgccaaaat gatgaataag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
     4681 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca
     4741 acgacagtag taccaagatc gatagcagca aaaaaagcca taatgaccat attgaagctg
     4801 tccgccaatc ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc
     4861 agaaccccca tcaacaggac acaggcattc tccatgaaga taggaacagc aagcggggtt
     4921 atctcgcgcc agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga
     4981 acaacctggc gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac
     5041 cacatttcaa ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
     5101 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tggggaattt aaaaatcggg
     5161 tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
     5221 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     5281 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
     5341 tcttcactgt tttccatcac ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa
     5401 agttccagaa agcgccagac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag
     5461 acagtattag catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt
     5521 tcgccgctgg attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta
     5581 atgacatcag aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat
     5641 gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc
     5701 gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc
     5761 aaactatggt gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     5821 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     5881 gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     5941 tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta
     6001 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     6061 gcaatgacca gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga
     6121 acaaatattt cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac
     6181 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     6241 cggattatct ttagttgttg gaaattcacg gtaaactccg ggcagttcag atttcccgtt
     6301 attgttaaag tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc
     6361 gatataacgc ttagctcacc aat
//
LOCUS       DAJVXP010000184         6360 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVXP010000184 DAJVXP010000000
VERSION     DAJVXP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.184"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..738)
                     /locus_tag="OQS10_003795"
     CDS             complement(<1..738)
                     /locus_tag="OQS10_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS2294705.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIVEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(879..2912)
                     /gene="metG"
                     /locus_tag="OQS10_003796"
     CDS             complement(879..2912)
                     /gene="metG"
                     /locus_tag="OQS10_003796"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS2294706.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3044..4153
                     /gene="apbC"
                     /locus_tag="OQS10_003797"
     CDS             3044..4153
                     /gene="apbC"
                     /locus_tag="OQS10_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS2294707.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4398..4679
                     /locus_tag="OQS10_003798"
     CDS             4398..4679
                     /locus_tag="OQS10_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS2294708.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4922..5464
                     /locus_tag="OQS10_003799"
     CDS             4922..5464
                     /locus_tag="OQS10_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2294709.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5544..6218
                     /locus_tag="OQS10_003800"
     CDS             5544..6218
                     /locus_tag="OQS10_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2294710.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
BASE COUNT         1672 a         1456 c         1520 g         1712 t
ORIGIN      
        1 aatatcaatc tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg
       61 gacgatctga tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc
      121 cgtgagtaca gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact
      181 gatcgcctgg gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc
      241 tgttttatta tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc
      301 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
      361 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      421 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      481 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      541 ctccacatac cccttcttca ccttctccac aatcagctta agctccgctt ttgccgctgc
      601 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      661 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      721 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      781 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      841 aagaaaaggc gctgtcgatg cagcgccttg aagggggatt atttcacctg atggcctggt
      901 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      961 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
     1021 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
     1081 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
     1141 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
     1201 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
     1261 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1321 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1381 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1441 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1501 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1561 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1621 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1681 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1741 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1801 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1861 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1921 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1981 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     2041 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     2101 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     2161 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     2221 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     2281 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2341 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2401 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2461 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2521 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2581 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2641 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2701 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2761 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2821 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2881 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2941 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     3001 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     3061 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     3121 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     3181 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     3241 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3301 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3361 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3421 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3481 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3541 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3601 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3661 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3721 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3781 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3841 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3901 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3961 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     4021 cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc
     4081 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     4141 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     4201 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     4261 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4321 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4381 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4441 gattggcttc tcccgcattc tcttcagaga ctgccacatt agcgattaat ggcaggatca
     4501 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4561 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4621 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4681 ttggcaacct gatttttatt gatatcaaca ataccattta cataaatcta cattaattga
     4741 tagatgcgat aaatagttaa tactcttaaa tatttgttgt atttatattg caatggttaa
     4801 tttgtttcaa ataaataaaa caatgtgttt acattgcggt aacttttttg atgtgagaaa
     4861 tttgtttcca agagatacag actcttaaca accaaaaaaa aattttatta aggaacatat
     4921 tatgaaacgt tcaattattg ctgccgctgt cttttcttct ttttttatga gcgctggagt
     4981 atttgctgca gacgttgata ccggaacatt aactattaag gggaatattg cagaatctcc
     5041 gtgtaaattc gaagcgggtg gtgattcagt aagtattaat atgccgactg taccaaccac
     5101 tgtctttgaa ggtaaagcta aatattctac ctatgatgat gcagtcggtg taaccagcag
     5161 catgttaaaa attagctgcc cgaaagaagt tgctggtgta aaactctcgt tgattaccaa
     5221 cgataaaata accggtaacg ataaggcgat agccagtagc aacgataccg tgggttacta
     5281 tctctattta ggtgataaca gcgatgtcct ggatgtttct gcacctttta acattgagag
     5341 ttataaaaca gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac
     5401 agataactca gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga
     5461 ttaataaata aaattgtaag tttctccagg taataaacat ggccgctatc ccatggcggc
     5521 cttttaattt aagaggcatt aaaatgaaag gattattatc tttactcatt ttttctatgg
     5581 tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac ccggcagaaa
     5641 ataaagaagt gatggtgcag ttgatgaacc agggaaaccg ttcttcgctg ctgcaggcgt
     5701 ggattgatga tggcgatacg tcattaccac cagaaaaaat tcaggttcct ttcatgttaa
     5761 cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc
     5821 cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg gatattccac
     5881 caaatagtcc agagcaagaa ggtaagaatg cactgaagtt tgcgatgcaa aacagaatta
     5941 agttgtttta ccgaccagcg ggtattgctc cggtaaataa agcgacattt aaaaaattgc
     6001 tggtaaatcg cagtggcaat ggtttggtga taaaaaatga ctcagctaat tgggtgacga
     6061 tttcggatgt caaagctaat aatgtcaaag tcaattatga aactattatg attgccccct
     6121 tagaaagtca gagtgttaat gtcaaaagta ataatgcaaa taactggcat ctgaccatta
     6181 tcgatgacca tggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga
     6241 gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag
     6301 ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa
//
LOCUS       DAJVXP010000185         6305 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVXP010000185 DAJVXP010000000
VERSION     DAJVXP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.185"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="OQS10_003801"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="OQS10_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS2294711.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="OQS10_003802"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="OQS10_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="OQS10_003803"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="OQS10_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="OQS10_003804"
     CDS             complement(5959..>6305)
                     /locus_tag="OQS10_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294712.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAJVXP010000186         6264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVXP010000186 DAJVXP010000000
VERSION     DAJVXP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.186"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..705
                     /locus_tag="OQS10_003805"
                     /pseudo
     CDS             1..705
                     /locus_tag="OQS10_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1458
                     /locus_tag="OQS10_003806"
     CDS             712..1458
                     /locus_tag="OQS10_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2294713.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1473..1895
                     /locus_tag="OQS10_003807"
     CDS             1473..1895
                     /locus_tag="OQS10_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS2294714.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1953..2309
                     /locus_tag="OQS10_003808"
     CDS             1953..2309
                     /locus_tag="OQS10_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294715.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2478..3143
                     /locus_tag="OQS10_003809"
     CDS             2478..3143
                     /locus_tag="OQS10_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS2294716.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3371..3526
                     /gene="hokD"
                     /locus_tag="OQS10_003810"
     CDS             3371..3526
                     /gene="hokD"
                     /locus_tag="OQS10_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS2294717.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3830..4102
                     /locus_tag="OQS10_003811"
     CDS             3830..4102
                     /locus_tag="OQS10_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294718.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            4176..5162
                     /locus_tag="OQS10_003812"
     CDS             4176..5162
                     /locus_tag="OQS10_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS2294719.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            5163..5543
                     /locus_tag="OQS10_003813"
     CDS             5163..5543
                     /locus_tag="OQS10_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS2294720.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            5540..>6264
                     /locus_tag="OQS10_003814"
     CDS             5540..>6264
                     /locus_tag="OQS10_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2294721.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT         1690 a         1409 c         1752 g         1413 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagagga agaggaagag gaagaggaag aggaagatct
      361 aaatactgat ctccccctcg ccaaaaacga gcgtctaaaa aattcgagcc ggaggctatt
      421 gagctgcccg attggttgcc ggaaacactc tggcatgagt gggtccggtt cagacaggca
      481 ttgcgaaaac cgattcgaac ggagcagggc gctaacgggg cgatacggga actggaaaaa
      541 ttccgtcagc agggttttac acctgagcag gtgattcgac acagcatcgc caatgaatac
      601 cagggcctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca ggttaacacc
      661 gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacggac catgaaaaat
      721 attgcgacag gcggcgttct ggaacgcatc cgccgactga ccccgccaca tgtaaccgcc
      781 ccattcagaa cggtagcgga gtggcgcgag tggcaacttg ctgaaggcca gaaacgtagc
      841 gaggagatca accgcctgaa tcgccagttg cgggtggaaa aaattctgaa tcgctcaggc
      901 atccagccgt tgcaccgtaa atgctcgttt gcgaattacc aggtgcagaa cgacggccag
      961 cgatacgcgt taagccaggc gaaatccatc gccgatgaac tgatgaccgg gtgtacaaat
     1021 tttgcgttca gcggaaaacc tggtaccggg aagaatcact tagcggcagc tatcgggaat
     1081 cgcctgctga aagacggtca gacagtgatt gtggttaccg tggctgatgt tatgagcgcc
     1141 ctgcacgcca gctatgacga cgggcagtca ggcgaaaaat ttttgcggga gctgtgcgaa
     1201 gtggatctgc tggttcttga tgaaattggc attcagcgcg agacgaaaaa cgagcaggtg
     1261 gtgctgcacc agattgttga tcgccggaca gcgtcgatgc gcagcgtggg gatgctgaca
     1321 aacctgaact atgaggtgat gaaaacattg ctcggcgagc gggtgatgga tcgcatggtc
     1381 atgaacggcg ggcgctgggt gaattttaac tgggagagct ggcgtccgaa tgttagccat
     1441 tcgagggttg ttaagtagtt tcaggaggat ttatggcgaa accttttact cccgaacagc
     1501 gggaagaact gaagacgcga attgtggaac tcgtgcatca ggacggtcgg gtcacgattc
     1561 ggcagttgtc agatgaaaca ggtatcagtc gtgcgtctgt cggtcgctta tgcatagaac
     1621 tggtcgcaag tggtgatgta tataattctg gctacggctt attcccgtct gaacaggctc
     1681 gcaaggactg gcaaagcgcc cgcaaaaaac tctcgagagt aaaggtgagg aaaccggttg
     1741 ttgttgatcc ggaccttatc tggtcattac ctgacggaga aatacgccgc tacgacaggc
     1801 gcctgaatat aatctgtcgc gagtgccgga agagcgaagc tatgcagcgt gtactggctt
     1861 tctatcaggg taattttcag gaggcgatac tgtgaatgaa attagctatc aggcttcaat
     1921 taccgctggc attcgcatca aaggagagga gcatggaaat aaaaccagaa gatgagttaa
     1981 gcaatatcgt tttatttccg gtaaaagagg atgaccctcg taatcaggtt aattttcttt
     2041 atgagccatc ggaaagacca tattgccatc acgcctctgt acgggttgac gaaaaagagc
     2101 gtcaggtccg ctgtaaaatc tgcggtgcgg ttgtggagcc gtttgactgg atgctctctg
     2161 tggcaaaaag agaaaccaga ctggcagatg atgtaaggct attgcgccag gaggaacagg
     2221 aaaggcggag aaatatagaa aagctgatac agattgagcg taacgcgaaa gcgcggatac
     2281 gcagggcgac aaaatccaga actgaataat taaatttagc actgttaaaa atttaatcct
     2341 taaccggagg gatttctgca ccctcaaatc atcaggagac cacccgaaag ggcggggagc
     2401 agtcacacat ctgtttccga tagccccgtt ctaatgctac actctttgat atttttatga
     2461 ccccaataaa catatttatg acagttgctg atttcaaacg gcccaaattg gagctcccaa
     2521 acggggcaaa caaactacta ctgcactctt gctgtgctcc atgttccggt gaggtgatgg
     2581 aggcgcttca ggcctcggga atcgactaca ccatcttttt ctacaacccg aacattcatc
     2641 ctcagaaaga gtatttaatt cgtaaggatg agaatattcg ctttgctgaa caacacggcg
     2701 tgccgtttat tgatgctgat tacgacacag acaactggtt tgaacgagcc aaaggaatgg
     2761 aatgggagcc cgaacgaggg atccgttgca ccatgtgttt tgacatgcgt tttgagcgga
     2821 cagcgctgta cgccgctgaa aatggtttca gtgtgatcag cagttcactg ggcatttcac
     2881 gctggaaaaa tatgcagcag gttaacgact gtgggcggcg agccgtcgcg cattatccgg
     2941 gcatggtgta ctgggattat aactggcgca agcagggcgg ctcgtcccgt atgattgaaa
     3001 tcagcaagcg cgaaaaattc tatcagcagg aatattgtgg ctgtgtgtat tctctgcgcg
     3061 ataccaatct acaccgcaaa tctcagggac gccctcttat caaaattggt caactccact
     3121 acggtaaaga agagaaggag tgattttatg gggcaccttt ctgattgatt tcatattggc
     3181 gaggtaagta gaatgactgc gggtgcttga ggctatctgc ttcaggcatg aacaccaaaa
     3241 ggcagataga gaaaagcccc agttaacatt acgcgtccgg caagacgctt aacattaatc
     3301 tgaggccaat ttcatgcttt gcacatgtag gttagcctct tacatgccga aaggcaagga
     3361 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
     3421 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtacgaa ttcgaaccgg
     3481 ccagacggag gtcgctgtct tcacagctta cgaacctgag gagtaagaga ccaggcgggg
     3541 gagaaatccc tcgccacctc tgatgggtca ggcatcctca atgcacccac acttaacccg
     3601 cttcggcggg tttttgtttt tattttcaac gcgtttgaag ttttagatgg tgccggaata
     3661 gaatcaaaaa tacttaagta gcgcgcaggg agaagaggga tggaccccga acaggggagt
     3721 gctatttatc tggaaggatt ctgttgatga aaatcgaaga attacgtgaa atttttagtg
     3781 aagatggcct ctatactgtg cgcgttgaga atggcgctat tgtcagccac tgccttccag
     3841 actacacaat tctgataatt cagccgtctt tgccagcagg cacgggcggc gctctcacgc
     3901 atttaaatct gattggtacc agcatccccc atgcactgaa gaacaggccg aatggctcat
     3961 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
     4021 ctggataatc tccgtcaggc tcccttactc cgaacggcca acgcgtccgt cccgcacatt
     4081 ccagcaacgg atctggaggt aatgtgcggg tattacttcg acctgttctg gtaccggaac
     4141 tcggtctggt tatcgttaag ccaggccgtg aatcaatgtc agcattccat aacggcagaa
     4201 tactggtgga gccggaacca aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
     4261 ctcgccagcc gctggtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     4321 ttcgtgctgc tggtggtgct ggtgcattgt ctgactggct gttgcgccat attaaatcct
     4381 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     4441 gcgcaatggt gttgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     4501 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     4561 taagcggtac gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca
     4621 atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg
     4681 aaaaaattcg ctctgtgtac agtgaaagcg acatcatacc gggagagcag acagccacca
     4741 gcatactgaa gcagcgcaca aaaaatattg cgttactgcc tcacgtccac cagcaacaga
     4801 acccaccaca ggaaaagacg gtggtcagca ttgccgttga tccggagtca ccggctcagt
     4861 atctccagcg ccagaaatca caacgggaag agatgcctgt atacacgcgc tgggtaaaaa
     4921 cgcagaaatg catgacgtgt ggcaatcagg cagatgatcc gcatcacatc attggtcatg
     4981 gactgggagg gatgggaaca aaggctgatg atttgtttgt tattccgtta tgccgtaaat
     5041 gccgtaacga actacatgcc ggagtaaaag attttgaaga gaaacacggc agtcagctgt
     5101 tgttgctgat tcgtttttta atgcacgcga gaaattcggg tgttctgaag tggaaagcat
     5161 aaatgaccga gcgcatagaa tttgttttac cttacccgcc gacggtgaac acttactggc
     5221 gtcgtcgtgg cagcacatat tttgtatcaa aagccgggga gcgttatcgc cgggcagtgg
     5281 cgcttattgt tcgccagcag cggctgaaat taagcctgtc cggaaggttg gcaataaaaa
     5341 ttattgcaga accaccggat aagcgccgcc gtgacctgga caatattctg aaagcgccgc
     5401 tggatgcgct gacgcatgcg gggttgctaa tggacgatga gcagtttgat gaaatcaata
     5461 tcgttcgtgc tcagccagta tctggtggac gtctgggggt gaagatttat cccataatgc
     5521 ttgaagggca ggtcaaaaaa tgaaactgga agatttaccg aaatactact ccccaaaatc
     5581 ccccggcctg actgatgcat cggcctcaac gtcgaaagat gcgctgagta tcactgatgt
     5641 gatggccgcg cagggcatga cacagaatcg ggctgagatg ggggtttctg cgttccttgg
     5701 gaaaatgggc attagtatga atgacagaga gcgggcaaca gaattgctga cagaatatgc
     5761 actcagtcgg tgtgatcgcg tggcggcgtt aagaaaactc ccggcagaaa taaaaccggc
     5821 agtgatgcgt attatggctt cgtatgcgtt tgaagattat gcccgtagcg cggcgagcaa
     5881 aaaacagtgc ccctgctgtc acggaaaaaa atttattgaa agcgaggttt ttacaaacaa
     5941 gatccagtat ccggatggta agccgccggt atgggcaaag tgtacgaaag gtgtgtatcc
     6001 gtcttactgg gaagaatgga aaaaagtcag ggaggtggta aaagttgcct gtccggagtg
     6061 tggcggaaag ggtgaggttt ccaccgcctg taaggattgc cgtgggcgtg gtgtcgccat
     6121 tcatcgtgaa gagtcggtaa aacgtggtat gcctgttatc agagactgcc agcgttgtgg
     6181 tggtcgtggc tatgaaagac taccatcaac ggaggcattt aatgctatat gcgaggtgac
     6241 aaaccagata acacgcgcgt catg
//
LOCUS       DAJVXP010000187         6200 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVXP010000187 DAJVXP010000000
VERSION     DAJVXP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.187"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..231
                     /gene="mcbR"
                     /locus_tag="OQS10_003815"
     CDS             <1..231
                     /gene="mcbR"
                     /locus_tag="OQS10_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS2294722.1"
                     /translation="SNMPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLL
                     AALKAKDKEGCRHCLAEIMQQNIAILYQQYNR"
     gene            complement(267..2369)
                     /locus_tag="OQS10_003816"
     CDS             complement(267..2369)
                     /locus_tag="OQS10_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS2294723.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2611..3672
                     /locus_tag="OQS10_003817"
     CDS             2611..3672
                     /locus_tag="OQS10_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS2294724.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3787..5286)
                     /gene="ansP"
                     /locus_tag="OQS10_003818"
     CDS             complement(3787..5286)
                     /gene="ansP"
                     /locus_tag="OQS10_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS2294725.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5553..6170
                     /locus_tag="OQS10_003819"
     CDS             5553..6170
                     /locus_tag="OQS10_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS2294726.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1661 a         1564 c         1485 g         1490 t
ORIGIN      
        1 tcaaatatgc ccatcctgtg tgagatgatt gagcaactgt gggtcaggat ggggcctggt
       61 ttacattatc tctatgaagc gattaatcca gcggaattac gggagcatat agaaaactat
      121 catctgttac tcgctgcgtt aaaagcaaaa gacaaagagg gatgcagaca ttgtcttgct
      181 gaaattatgc aacaaaatat tgctatttta taccagcaat acaatcgtta aagtaaatgc
      241 ccggcaaata ccgggcatca aggtaattac tcaaatctcc acgcaatatt catgccgaca
      301 ccataatttc gtccgggcga aggttcgtaa tatcgcccgt ttgactcatt gacaatgaca
      361 gaaccaacgt attctttatc gaataaatta tcgacacgac caaataaatc cacagttaaa
      421 ttgtggtaat tgtatttata cccggtgaat aagccgacga gagtataaga cggcgctttt
      481 gccgtatttt catcatctgc cataatatcg cccatataac gcgcttccgt gcctgcatac
      541 caaccatctt ccggtacata acctatcgac gcaaagccca tattacgggc gatccctggc
      601 atccgattac cgttacaatc ctgttcattg caaacattgc tgcgataggt cgcatcaagc
      661 caggtccagg acgcgtttac gcgaaaatct cctgcgaaac gttgatccca tgccagttca
      721 gcgccttgac gacgggtctt tccggcattt ttgtaagtcg tacgcccacc gctactgcta
      781 tcgacaacaa tttcatcatc agtgtcggtc tgaaacaatg cgagactaag cagcccatca
      841 ccaatacgcg ttttactgcc gatctcaatt gtatcgttgg tggatggttt taaaccaaag
      901 ttcataccgc tttgcccatc agcacgataa gacagctcat taatcgtcgg cgtttcaaaa
      961 cctcgcccgg ctgccagata gatattccag gcatcggtca ttgcatattt taacgaaccg
     1021 gcaggtagcc atttatgata actggcatca ccgctgtcat cgccgttacc cggagtaacg
     1081 taatggtcgt tggaatcaaa ccacacggag ctgtagcgca cgccagcatc cagcgacagt
     1141 ttttcgctca gctgccactg cgtctgtaaa tagggatcga tgttccacat cagattgcgt
     1201 tcgtcgcgac gcaactcacc tttttgcccg tactccggca tgccgctatt caggcggaag
     1261 ttattgtagc ccttgcggtt ttcactcatg ttttcgtagt tcaggccggt agtgaacgtg
     1321 accggaacgc ccagttcacc acggtgtgtc cagcggctgt ctatcccctg gtaatggcgt
     1381 tgcagggtaa tcacgccgcc cgcatgtgac gggttaagtt gtggtgccat gggtattgac
     1441 tggtactggg tcgtttctcg ctctccggca tacatcatca cactcatatc atcttgcgcg
     1501 ctcaggctac gctcatagcg caacccagcc tgagtttgct tgatggtttt tcgcgtgtcg
     1561 tactgttctg cacgaggcgc ttgttgtgga ttagccttcc attctgcttt ggttagccca
     1621 cccgggtcat ctgctttgat atccacacta ttgaaaatca gacttaattt gctggcttca
     1681 tcaatgcgta cgcccagttt ggcattggct aaatttttct gtgcgccact atggtcacga
     1741 tagccgtggg tcgtaaaacg cgtggttgag acggtgtaat cgacatcgcc aggctgtgtg
     1801 ccgtctcccg ttgcgcccgt tgctttcagc ccatagcgcc agctgccaaa actgccgtag
     1861 taactactgg cttcaatggt tggtggctgt tgtccggtct gggtggtgac attcattacc
     1921 ccaccagacg cgttgccata cagggcagag aaggggccac gcagcacttc cacattttgc
     1981 acactgctta aatcgatgtt ggatgtttgc ccttgcccgt cgggcatggt ggcgggaata
     2041 ccgtccacat acaggcgaat accgcgaata ccgtaagtgg agcgggagcc aaatccgcga
     2101 atcgacagct gtaaatcttg cgcatagttc tgccggtttt gtacctgcaa accaggcacg
     2161 tcggtcagtg attcggacaa gttaatgcgc ggtgttgcca ggcgcatctc ctcgccatcc
     2221 accacgctta ctgctgctgg ggtatccagt tctgaaacca cctgcggtgc ggcactgaca
     2281 atcatggtct gttcatcagc ggcaaaaaca acgggggaaa gggcaagtag tgcgggcaaa
     2341 acggtctgtc ggacggaaaa aatcttcatg aaaaaagcca ggttaagaat gggaaaacgc
     2401 cgttatggta ataaaattgt aaattcatgg aaaacgaaac ggcacgatac gttaagtaat
     2461 tgagaaaatt gtagtcgtaa cggcaagaaa tgctccacat ttgagaaaat aatgattacc
     2521 attcccattt ataacaagag cgtaacgatg attacgctta gcgaagcatt gtgaagcagc
     2581 aaaaatatcg gttcatcaaa gggagtcgtc atgcatttac gtcatctgtt ttcatcgcgc
     2641 ctgcgtggtt cattactgtt aggttcattg cttgttgctt catcattcag tacgcaggcc
     2701 gcagaagaaa tgctgcgtaa agcggtaggt aaaggtgcct acgaaatggc ttatagccag
     2761 caagaaaacg cgctgtggct cgccacttcg caaagccgca aactggataa aggcggcgtg
     2821 gtttatcgtc ttgatccggt cactctggaa gtgacgcagg cgatccataa cgatctcaag
     2881 ccgtttggtg tcaccatcaa taacacgact cagacactgt ggtttggtaa cactgtaaac
     2941 agcgcggtca cggcgataga tgccaaaacg ggcgaggtga aaggccgtct ggtgctggat
     3001 gatcgtaagc gtacggaaga ggtgcgcccg ctgcaaccgc gtgagctggt agctgacgat
     3061 gccacgaaca ccgtttacat cagtggtatt ggtaaagaga gcgtgatttg ggtcgttgat
     3121 ggcgagaata tcaaactgaa aaccgccatc cagaacaccg gtaaaatgag taccggtctg
     3181 gcgcttgata gcaaaggcaa acgtctttac accactaacg ctgacggcga attgattacc
     3241 atcgacaccg ccgacaataa aatcctcagc cgtaaaaagc tgctggatga cggcaaagag
     3301 cacttcttta tcaacattag ccttgatacc gctaggcagc gtgcatttat caccgattct
     3361 aaagccgcag aagtgttagt ggtcgatacc cgtaatggca atattctggc gaaggttgca
     3421 gcaccggaat cactggctgt gctgtttaac ccagcgcgta atgaagccta cgtaacgcat
     3481 cgtcaggcag gtaaagtcag tgtgattgac gcgaaaagct ataaagtagt gaaaacgttc
     3541 gatacgccga ctcatccaaa cagcctggcg ctgtctgccg atggcaaaac gctgtatgtc
     3601 agtgtgaaac aaaaatccac taaacagcag gaagctaccc agccagacga tgtgattcgt
     3661 attgcgctgt aatagatatg gcgagggtga aaacggagag acttgcctga cttttatcac
     3721 gccgcatgcg gcacaccttc gtcagtaatc taaatttgcc cggcacgtag ccgggcaatt
     3781 ttgcaattag ctcgccgttt caggcttaaa cacaatttcc tgtttttctt catcttcctc
     3841 gacgactggc gcagtgctgt gaatttcagc aacgcgtttg cgcacaccaa accagcctat
     3901 aaccagcaga ataccgataa tcggcagcgc cgcgatagta taagtcccgt tcgggtaatc
     3961 gaacgccatc agcacaagga cactcagtaa aaacagtaat gtcagccagg aagtgaaggg
     4021 cgcgccaggc agtttaaaac tgacatccgc tgctttgcct tctttaatcg ctttacgcag
     4081 gcgcatctgg cacacgatga taaacgccca tgaagcgatg attcccagcg acgcgaagtt
     4141 caacacaatc tcaaatacgc gcgacggcac cagatagttg aggaatacgc cgacgacata
     4201 cacaaccagt gtcgccagaa tcccggcata cggcacatgc tgacgactca ttttcgccat
     4261 aaaactcggt gcggaaccgc ccatcgccat cgagcgcaga atacgcccgg tgcagtacag
     4321 acctgagttc aggctggaga gggcagcggt cagcaccaca atgttcataa tgctgccgat
     4381 atatggcaca cccagtttag agaaaaacgt cacgaacgga ctttgccccg cctgatacgc
     4441 gctccacggc aataacataa ccagcaacac cacggagccg acgtaaaaca ggccaatacg
     4501 ccaaatcaca ctgttaatgg ctttaggcac catggtctgc ggatctttac attctccggc
     4561 agctgtaccc accatttcaa tggaggcaaa agcaaacact acgccctgaa tcaacaccag
     4621 cgcaggcagc aaaccgtggg ggaagaagcc gccattatcg gtgattaaat gaaagccagt
     4681 ggtgttgcca tccagcggct gaccactacc gaggaacact gtacccacga ccaaaaaggt
     4741 cacaatggcg agcactttaa taagcgcaaa ccagaactcc atctccgcaa accatttcac
     4801 accgatcata ttcatggtgc caacgatggt aagtgcagcg agcgcaaaga cccactgcgg
     4861 cacgccgcca aacgcacccc agtaatgcat atacagagcg acggcggtaa tatcaacaat
     4921 ccccgtcatc gcccagttga tgaagtacat ccagccagca acataagcgg ctttctcacc
     4981 caaaaactca cgggcataag agacaaaact gccactggaa gggcggtgta gcaccagctc
     5041 acccaatgca cgcagaataa aaaacgaaaa caagccacaa attaaataaa ccagtgccag
     5101 tgctggaccc gccatttgca gtcgggctcc tgcacctaaa aacaagccgg tgccaatcgc
     5161 gccgccaatg gcgatcatct gtacctggcg attgcccatc gctttgtgat acccctcttc
     5221 gtgggcatta agccagcggc gtttcgcggc gtgttgatct gaagtgtcgg tgtcgtgttt
     5281 actcattgct ctccctgatt gctttaatga aaaagtcata taagttgcca tgaacaatgt
     5341 ttattcatcc tggcgatgat agttatcacc attattcttt atgggtataa aactatcggc
     5401 gcagcatcct acccgttatc gatatacgat gcaaaacatc cccttacaat cctgaagggg
     5461 attcatacaa ctgacgaaaa aatgacaaat acttttgctg gttaacctgt gtactgtcct
     5521 acacttaatc tttaaaagat tgtgaggggc atatgattaa ggtgtatggc gtacccggat
     5581 ggggctctac aatcagtgag ctgatgctaa cactggctga tattccttat cagttcgttg
     5641 atgtcagtgg ttttgaccac gagggaactt cacgcgagtt attgaaaacc ctgaacccgt
     5701 tgtgtcagat tccgacactg gcgctggaaa atgacgagat tatgacggag acagcggcga
     5761 ttgcattgat ggttctcgat cgtcgcccgg accttgcgcc accggttggg cgcgccgaac
     5821 gtcagcagtt tcaacggcta ttggtctggc tggttgctaa tgtctatcca acattcactt
     5881 tcgccgatta ccctgagcgt tgggctcctg acgcacctga acagttaaag aagaatgtta
     5941 ttgaatatcg taaatcgctt tatatctggc tgaattcaca attgaccgct gaaccttatg
     6001 cgtttggtga gcaactaacg ctggtggact gttatctttg cactatgcgc acatgggggt
     6061 ctgggcatga atggtttcag gataatgcca cgaatatcag tgcgattgct gatgctgtat
     6121 gtcagttacc gaaattacaa gaagtattaa aaaggaatga aattatttga gtgggatacc
     6181 agaggattat gttatttatt
//
LOCUS       DAJVXP010000188         6194 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVXP010000188 DAJVXP010000000
VERSION     DAJVXP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.188"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            165..319
                     /locus_tag="OQS10_003820"
                     /pseudo
     CDS             165..319
                     /locus_tag="OQS10_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            365..781
                     /gene="hicB"
                     /locus_tag="OQS10_003821"
     CDS             365..781
                     /gene="hicB"
                     /locus_tag="OQS10_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCS2294727.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            860..2266
                     /locus_tag="OQS10_003822"
     CDS             860..2266
                     /locus_tag="OQS10_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCS2294728.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2511..3656
                     /locus_tag="OQS10_003823"
     CDS             2511..3656
                     /locus_tag="OQS10_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2294729.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3674..4687
                     /locus_tag="OQS10_003824"
     CDS             3674..4687
                     /locus_tag="OQS10_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2294730.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4688..5588
                     /locus_tag="OQS10_003825"
                     /pseudo
     CDS             4688..5588
                     /locus_tag="OQS10_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5578..>6194
                     /locus_tag="OQS10_003826"
     CDS             5578..>6194
                     /locus_tag="OQS10_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2294731.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEI"
BASE COUNT         1428 a         1507 c         1664 g         1595 t
ORIGIN      
        1 tattcagttt tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg attttgcatt
       61 gcattctgcc gttgcggcga tttagtgcta ttttcgagca aattacacat aggggtaaac
      121 actaatgttt actttattga tatactcagc ggcagggagg cgatgtgaaa caaagcgagt
      181 tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa tggccgcaac catttgaaac
      241 tcaggtttca tgggaggcgc agtgtcatgc cgcgtcactc ctgcgtaaag caatcctgaa
      301 acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag agacagtcga
      361 aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata tggtttcttt
      421 tgtggatatc cctgaagcgt tgacgcaggg cgaaactgtc gctgaagcga tggaagcggc
      481 aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc ttatcccgtt
      541 accttcgcca ttaaatagtc atgatcactt tattgaagta cctttgagcg tcgcctctaa
      601 agtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt tagccaggcg
      661 aattggcaaa cctaaacagg agattactcg cctatttaac ttgcaccatg cgacaaaaat
      721 cgacgccgtc cagcttgcgg caaaggcgct tggcaaagag ttatcgctgg tgatggttta
      781 attacagtta acgaaaagtt gtcattttta acaactgata tagactgccg aatcatctgc
      841 acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat tacgcgagca
      901 gattgcgtcg ggtatctggc aacccggcga tcgtttgcct tcgttgcgtg accaggtggc
      961 gctttcaggc atgagcttta tgactgtcag ccatgcctat cagttgctcg aaagtcaggg
     1021 atatattatc gcacgaccgc agtcgggtta ttacgttgcg ccacaggcaa taaaaatgcc
     1081 gaaagcgcca gtcattccag tcactcgaga tgaagcagtc gatatcaaca cttatatttt
     1141 tgatatgttg caggccagtc gcgaaccgtc ggtcgttccg tttgcctcgg cctttcccga
     1201 cccgcgactt ttccccctcc aacaactaaa ccgctcgctg gcgcaggtaa gcaaaaccgc
     1261 cacagcgatg agcgtgattg aaaacttacc gccaggaaac gcagaactgc gtcaggctat
     1321 tgctcgtcgc tatgccttac agggcatcac catttctcct gatgaaattg tcattactgc
     1381 cggggcgtta gaggcattaa acctcagttt gcaagcggta actgaaccgg gcgattgggt
     1441 gatagtagag aatccttgtt tctacggtgc gttgcaggcg ctggagcggc tacggctgaa
     1501 ggcgttatcg gtggcgacgg atgttaaaga agggatcgat cttcaggcgc tggaactggc
     1561 gttgcaggat tatccggtga aagcgtgctg gctgatgact aatagccaga acccactcgg
     1621 atttacctta acgccgcaaa aaaaagcaca actggtggcg ttactcaatc agtacaacgt
     1681 aacgctgatt gaagatgacg tttacagcga actttatttt ggacgggaaa aaccgctgcc
     1741 tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt cgaaatgtct
     1801 ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcacgta aaattcaacg
     1861 cttgcagttg atgagtacgc tttccaccag ctcaccgatg caacttgcgc tggtggatta
     1921 cctttccacg cgccgatacg acgcccatct tcgtcgcctg cgtcgccagc ttgcggaacg
     1981 taaacaacgt gcctggcagg cactgctgcg ttatctgcct gcggaagtta aaattcatca
     2041 tagtgacagc ggttattttc tctggctgga gctccccgag ccgttagatg ccggcgaatt
     2101 aagcctggcg gcactgacgc atcatatcag tattgcgccg ggtaaaatgt tttctaccgg
     2161 tgaaaactgg tcacgttttt ttcgttttaa taccgcgtgg cagtggggag aacgtgaaga
     2221 acaggcggta aaacaattag gcaaacttat tcaagaacgg ctgtaatagc gtttaattta
     2281 attcctctta gattgggtaa tatgaatttc gaatagcagt catatttcct aaccccttgt
     2341 ctatactcca gaagataacc ttacagacgg cataatgcgc ggtagctcac aacctgatta
     2401 aattttctca gggacgaagg tgtgcctgca agccgccgtc tatggttaaa caaggagata
     2461 tttttacggc acggcggctg aacaattaat tacgacagga gtaagacctt atgagcaaga
     2521 catttgcccg cagcagcctg tgtgcgctca ccatgacaat aatgaccgct cacgccgccg
     2581 aaccacctac caatttagat aaaccggaag ggcgactgga tattatcgcc tggccgggat
     2641 acatcgaacg cggacaaact gataaacaat acgactgggt aacgcaattt gaaaaagaga
     2701 caggctgcgc ggtgaatgtg aaaaccgccg cgacttccga tgaaatggtc agtctgatga
     2761 ccaaaggggg ttacgatctg gttacggcat ccggcgatgc ctcgctgcgt ttgattatgg
     2821 gcaaacgcgt gcagccgatt aataccgcct tgattcccaa ctggaaaacg ctcgatccgc
     2881 gcgtggttaa aggcgactgg tttaatgttg gcggcaaagt ttacggcaca ccttaccaat
     2941 gggggccgaa cctgctgatg tacaacacta aaaccttccc gacgccgccg gatagctggc
     3001 aagtggtttt tgttgagcaa aatctgccgg acggcaagag caataaaggc cgcgttcagg
     3061 cttatgatgg ccctatctac attgcggacg ctgcgttgtt cgttaaagcc actcagccgc
     3121 agttgggcat cagcgatccg tatcaactaa ccgaagaaca gtaccaggcg gtgctgaaag
     3181 tgctgcgcgc tcaacacagt ttgatccatc gctactggca tgacactacc gtgcaaatga
     3241 gcgatttcaa aaacgagggt gtggttgctt ccagtgcctg gccctatcag gccaacgccc
     3301 tgaaagccga aggccagcct gttgctaccg ttttcccgaa ggagggtgtt accggttggg
     3361 ctgataccac catgctgcat agcgaagcga aacatccggt ttgcgcctac aaatggatga
     3421 actggtcatt aacgccaaaa gtgcagggcg atgtggcggc ctggtttggc tcgttaccgg
     3481 tagtgccgga agggtgtaaa gccagtccgt tattaggcga aaaaggttgt gaaaccaacg
     3541 gttttaacta tttcgacaaa atcgccttct ggaaaacgcc tatagcagaa gggggcaagt
     3601 ttgttcccta cagtcgctgg acgcaggatt acattgccat tatgggcggt cgctaacttc
     3661 gctggggtgc tttatgacgt acgcagtgga gtttgacaac gtctcgcggt tgtacggtga
     3721 cgtgcgcgca gtagatggcg tcagtattgc gataaaagat ggtgagttct tctctatgct
     3781 ggggccgtcc ggctccggca aaaccacctg cctgcgcctg attgctggct tcgaacagct
     3841 ttccggcggg gctatctcta tctttggtaa acccgccagc aatctgccac cgtgggagcg
     3901 ggacgtgaat actgtctttc aggactacgc gctatttccg catatgtcga ttcttgacaa
     3961 tgtcgcctat gggctgatgg tcaaaggcgt gaataaaaag cagcggcacg caatggcgca
     4021 agaggcgctg gagaaagtgg cgttggggtt tgtacagcaa cgtaaaccgt cacaactttc
     4081 tggtggtcag cgccagcggg ttgctatcgc cagagcattg gtgaatgaac cgcgcgtatt
     4141 gctgttggat gaaccgctcg gcgcactgga tctcaaattg cgtgagcaga tgcagctgga
     4201 actgaaaaaa ctgcaacagt ctctcggtat cacttttatc ttcgttaccc acgatcaggg
     4261 cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat aatggacgca ttgagcaggt
     4321 cgattccccg cgcgatctct atatgcgccc gcgcacgccg tttgttgccg ggttcgttgg
     4381 tacatcgaat gtttttgatg gactgatggc agagaaactt tgtggcatga cgggaagctt
     4441 cgccctgcga ccggaacata tccgcctcaa cacccctggt gaactgcagg ccaatggcac
     4501 gatccaggcg gtgcaatatc agggcgcggc aactcgtttt gaactgaaat tgaacggcgg
     4561 tgaaaaactg cttgtgagtc aggccaatat gacaggcgaa gaactgcctg ccacgctcac
     4621 gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg atggtgccgc tggttgagga
     4681 gaggtgaatg gcgatgaatg tattgcaatc accttcacgt ccaggtctgg ggctgttttt
     4741 actgctgctt ggcccgctaa tgtggtttgg cattgtctat ttcggctcgc tgctgacact
     4801 gttatggcag ggattttata cttttgacga tttcaccatg tcggtaacgc cggaactgac
     4861 gctggcgaat atccgtgcgc tgtttaatcc ggcgaattac gacatcattc tccgcacgct
     4921 gactatggct gtggcagtca ctatcgccag cgccattctg gcttttccaa tggcgtggta
     4981 tatggcgcgc tataccagcg ggaaaatgaa agcgttttta tattgcggta atgttgccga
     5041 tgtgggcgag ctacattgtt aaagcctatg cctggacgtt attgctggca aaagatggcg
     5101 tggctcagtg gtttttacaa catcttgggc tggaaccact gctgactgcg ttccttacat
     5161 tacctgcggt gggcggaaat acgctgtcaa cttccgggct ggggcgcttt ctggtgtttc
     5221 tctatatctg gttgccgttc atgatcctgc ccgttcaggc ggcgcttgag cgtttgccgc
     5281 cgtcattgtt gcaggcgtcg gctgatctcg gcgcacgtcc acgacaaacc tttcgctatg
     5341 tggtgctgcc gctggcaatc ccgggtattg ccgctggctc tatctttacc ttctcactca
     5401 ctctgggcga ttttatcgtc ccgcagctgg ttggtcctcc aggatatttt atcggcaata
     5461 tggtttattc ccagcagggg gcgattggca atatgccgat ggcggcggca ttcaccttgg
     5521 tgccgattat tctcatcgca ctgtacctgg cgttcgtgaa acgtctggga gcgttcgatg
     5581 cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt tgttttccta
     5641 cattttccca tcctgataat cgccgcctat gcgtttaaca ctgaagatgc ggcgtttagt
     5701 tttccaccgc agggcctgac gctgcgctgg tttagcgtgg cagcacagcg tagtgatatt
     5761 cttgatgccg tgacactgtc acttaaagtg gcggcgctgg cgacattaat tgcgctggtg
     5821 ttagggacgc tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa aaacgccatt
     5881 tcgctgttac tgctgctgcc cattgcgctg ccgggcagcg tcactggtct ggcgttatta
     5941 accgccttta aaaccatcaa tctggagccg ggatttttca ccatcgtggt cggtcatgcg
     6001 actttttgtg tagttgtggt gtttaacaat gtcatcgccc gttttcgccg cacctcctgg
     6061 agtctggttg aggcgtcaat ggatcttggg gccaatggct ggcaaacctt ccgctacgta
     6121 gtgttgccga atctcagttc ggcgttactg gcaggaggaa tgctggcgtt tgccttgtcg
     6181 ttcgatgaaa tcat
//
LOCUS       DAJVXP010000189         6034 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVXP010000189 DAJVXP010000000
VERSION     DAJVXP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.189"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            178..411
                     /locus_tag="OQS10_003827"
     CDS             178..411
                     /locus_tag="OQS10_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS2294732.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(444..1082)
                     /gene="ompW"
                     /locus_tag="OQS10_003828"
     CDS             complement(444..1082)
                     /gene="ompW"
                     /locus_tag="OQS10_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS2294733.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1322..1411
                     /locus_tag="OQS10_003829"
                     /pseudo
     CDS             1322..1411
                     /locus_tag="OQS10_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1439..2182
                     /locus_tag="OQS10_003830"
     CDS             1439..2182
                     /locus_tag="OQS10_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS2294734.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2212..2751
                     /locus_tag="OQS10_003831"
     CDS             2212..2751
                     /locus_tag="OQS10_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS2294735.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            2856..3254
                     /gene="yciA"
                     /locus_tag="OQS10_003832"
     CDS             2856..3254
                     /gene="yciA"
                     /locus_tag="OQS10_003832"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS2294736.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3294..4013)
                     /gene="tonB"
                     /locus_tag="OQS10_003833"
     CDS             complement(3294..4013)
                     /gene="tonB"
                     /locus_tag="OQS10_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS2294737.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4104..5624
                     /locus_tag="OQS10_003834"
     CDS             4104..5624
                     /locus_tag="OQS10_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2294738.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(5657..>6034)
                     /locus_tag="OQS10_003835"
     CDS             complement(5657..>6034)
                     /locus_tag="OQS10_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294739.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1546 a         1409 c         1446 g         1633 t
ORIGIN      
        1 ttaatccgta taaggaaaac tgatatggca actttgacaa aaaaagaacg ggcatggttg
       61 aacgaattac aggaagttct tgatcgctgt cctgtggtgt catcctgccg cgattgcgtt
      121 cttagtccgt ggctattgca ccaccatcac gcgaaaggga cagctaagcg cggcgggatg
      181 gttaagccag caacattaac cgttgcattt aaaaaagccc gggattctgt ggattacaac
      241 tggcgtgcta atggcacccc accctctttc catgagcaga gatctttatc agagcgattg
      301 ttcagagagc agggggttga taccaaaatt ttgctaggcc attcgaatca aaaaatgacc
      361 gatatttaca acgatgtacg cggtaaggag tggaaaaaac tggtcatttg ataaccagtt
      421 ttgcagaggt tttgcagaga aaattaaaag cggtacccgg cagagaacat aaacacccac
      481 ggatccagtc gtaccgagtc ttttacggta gtaacaccag atttgtactt agccgtggta
      541 tcgatatcca tgtaccacac tgacatgtta accaaccagt cacggttaat cagataatca
      601 acccccacct gcccggcagc tccccagtaa tctttcagac tgagatcgga aagccccgcc
      661 tctttgccat gatcgttaaa tccattatca aagaaggtgg tgtagttaat acctgcccca
      721 acataaggac ggaatttgct gccggcatca ccaaaatacc actgcgccat cagtgttggc
      781 ggcagatgat gaacggttgc aatatcgccg gtcgcccggg tgccgatttt atggcggaac
      841 ggcgtcgctg ccagtaattc cacaccaatg ttgtcggtcg ccatataagt aaacgtcagg
      901 cccagttgcg tgttattggt cacgctgaat ccacccagac ttcctaacgt accaccagca
      961 ccttctgttg gacgtacggt tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc
     1021 gcaaaggcac tgccagagag aagagttgtt actgccaaag ccgccactgt taactttttc
     1081 atatccgctc cgtcgttatg gttataaaaa cgtgacgaat atacctacat tggagtaaca
     1141 aatgatccta catagatcac attacatatg ataattcacc ctttattgat ctggattaat
     1201 ttagaagttg cattgagaat cctggttaac gtgatttaaa tcaattttta gatattttcg
     1261 ctacgcaaat attaactcgt tcgccacttg ccgcggtttg cccacgcctg aaggccagca
     1321 attacctccg ctttacaaag atatgctttt ccatacaaga cctgatgctg ccacccgagg
     1381 cttaaccagg gtacaattgc ccgctaatta acacctgcat aaaactcaag gagagtgcat
     1441 gtctatcacg gcgcagtccg tataccgtga caccggaaat ttcttccgta atcaatttat
     1501 gaccattctg ttggtatcgt tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt
     1561 ctcacccagt gatgcacagc ttgcgcagct caatgacggc gtgcccgtta gcggcagtag
     1621 tgggttgttc gacctggttc agaatatgtc accggaacaa caacaaattt tgctgcaggc
     1681 ttcagcggcg tccacttttt caggattaat cggtaacgcc attctcgccg gaggcgtaat
     1741 attaattatc cagctggtgt ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc
     1801 cagtgcgccg atattgccca agttatttat tctgattttt ctgactaccc ttttagtaca
     1861 gattggcatc atgctggtgg tcgttccggg aattatcatg gccattttac tggccctggc
     1921 accggtgatg ctggtacagg acaaaatggg catttttgcc tcgatgcgta gcagtatgcg
     1981 gctgacttgg gcgaatatgc gtctggtggc acccgcagta ctgagctggt tgctggcaaa
     2041 aacactgttg ctgctttttg cctcttcttt tgccgcatta accccggaaa ttggtgccgt
     2101 actggcgaac accttgagca acctgatttc agccatattg ctcatctatc tgttccgcct
     2161 gtatatgttg attcgccaat aaccttaagc atttgattac ggaatcgtaa aatgaagcag
     2221 tttcttgatt ttttaccgct ggttgtcttt ttcgcgtttt acaagattta tgacatctat
     2281 gcggctactg cggcgctgat cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc
     2341 tttcgtaagg ttgagaagat ggccctgatc acttttgttc tggtggtcgt cttcggtggc
     2401 ttgacgctgt tcttccacaa tgatgagttt attaaatgga aggttacagt catttatgct
     2461 ctgtttgcgg gagccctgtt agtcagccaa tgggtgatga aaaagccgct aattcagcgg
     2521 atgctgggta aagaactcac gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg
     2581 gctgttttct ttatcctttg cggtctggca aacatctaca tcgcattctg gctgccgcaa
     2641 aatatttggg tcaactttaa agtctttggc ctgaccgccc ttaccttaat cttcacattg
     2701 ttaagcggta tctatatcta ccgccacatg ccgcaggaag ataaatccta accagactgc
     2761 cagtccttcg gggctggcgc tgtatctcct ctgttctcct caggaaaatc atagtagcat
     2821 cgcgcctgtg attttccttt taagtcggtt ttaccatgtc tacaacacat aacgtccctc
     2881 agggcgatct tgttttacgt actttagcca tgcccgccga taccaatgcc aatggtgaca
     2941 tctttggtgg ttggttaatg tcacaaatgg atattggcgg cgctattctg gcaaaagaaa
     3001 ttgcccacgg tcgcgtagtg actgtgcggg ttgaaggaat gactttctta cggccggttg
     3061 cggtcggcga tgtggtgtgc tgctatgcac gctgtgtcca gaaagggacg acatcggtca
     3121 gcattaatat tgaagtgtgg gtgaaaaaag tagcgtctga accaatcggg caacgctata
     3181 aagcgacaga agcattattt aagtatgtcg cggttgatcc tgaaggaaaa cctcgcgcct
     3241 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tacttactga
     3301 atttcggtgg tgccgttaat tttaaacagg atattcacca caatcccact gcctggctta
     3361 cccggctcat aacgccatct gcgcatcgca tttttcacct cacgctcaaa catgttcgca
     3421 ggcttggctg agaggatttg tacgttatcc acgcgaccat ccggcgtgac gtcaaattta
     3481 actttaacct gcccttcaat gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta
     3541 cggcttaatg cgcgtggtcc tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt
     3601 gccgtacttg atgtggggcg cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac
     3661 tctacgggtt tgacatcgcg ttttggctgc tcctgtacct ttttcaccgg cttcggtttt
     3721 ggcttaggtt tcggcttcgg cttttcaatg accaccggtg cttcttttgg cggttcgggg
     3781 atcggctcag gttccggttc tggctctacc accggctccg gtggcggctg aacggcttgt
     3841 ggcggttcga gatcagcagg cgcaaccatc gtgacagaaa ttggctgcgc aggcgcaggt
     3901 agttcaataa cctgatgtac cgaggtatag agcagacccg ccacaacagc accatgaatg
     3961 cagaccgaaa gtaacgtcgg ccaggggaag cggcgaggta aatcaagggt cattgtattg
     4021 tagctattcg gcattgtagc attagccata acgtttataa gttcatcttt aaatgataat
     4081 caattgcatg ggagtttatc accgtgagaa ccgtaataac atacttgaga ttctcatcag
     4141 ccatacaagg cgctgaaggt gcagattcaa ccagacgaca aaacgacctg ttcaagcagt
     4201 ggctgaagaa gaatggcgat gctcaaatag tagcgtcttt cagtgatgaa gggttgagtg
     4261 gttacaaagg taaacacctt actggtcagt ttggtgacat gttagcccgt attgaggctg
     4321 gggagtttcc agaaggtaca attctgctag ttgagagtat cgaccgtata ggccgattag
     4381 agcatcttga aacagaagcc ttgatgaacc gcatcttagg caacgggata gagattcaca
     4441 cattgcaaga tggccttatc tacacaaagg atgctttagc cgatgacctt ggcataagca
     4501 tcatccagcg tgtcaaagcc tacatagcgc atcagaaatc taagcagaag tctttccgtg
     4561 tttctcagaa atgggggcaa cgtgcaaaac ttgcccttgc tggtgaacaa cgtcttacaa
     4621 agatggttcc tggatggatt gaccctgaga cttttaaact caatgaacac gctgagactg
     4681 tgagattgat attcaaactg ctgctagatg gtgaaagtct gcataacatt gcacgtcacc
     4741 ttcaaagcaa cggtataaag tcgtttagtc gccgtaaaga tgctaatggg ttctctgttc
     4801 actctgtacg caccattcta aggtcagaga caacaatagg cacgttacca gcatcacaac
     4861 gtaatgaccg ccccgctata ccgaactact acgaaggtgt tgtagatata ccaacgttca
     4921 ataaagctca agagattctc gacaagaatc gtaaaggccg tacacctgca agtgacaacc
     4981 cactaacgat taacatcttc aaaggtctgt ttaggtgtca gtgtggggct agtgtccatc
     5041 ctaccggaac aaagaataag tatgctgggg tctacaggtg caataaccac ttagacggtc
     5101 gctgtgatgt tccaccgttg aagcgtaaac cgtttgaccg atggatgatt gataattttc
     5161 tggggatgat tgacgtgggg aatgatggag aatcagagag aaagattgca gcgttacagc
     5221 atgaggttga aattgtcaca gccagaatca agaaagctac cgccctactt cttgagatgg
     5281 atgatattga tgaactaaaa attcagctta aggaactgaa ccagaagcgc acagaactac
     5341 agaccacgat tgataacatg aggcgtaagg cttcactcac tgataaggaa ctaccccagc
     5401 ttaaagatat tgaccttatg actaaagcgg gtcgtgtcga gtgtcagttg attttgtcca
     5461 agcatctaaa agggcttaca ttgggtaagg attcggtaac tgtaacgcta cagaacgaca
     5521 ctgaaatcac catacctaca aacccgttac cgctaaatga tgggtcacca atctttgaaa
     5581 ttgctgataa agagctactg gatattgacg cttatcaact gtaattttca ggggctatga
     5641 agcccctttt tatgtctcaa taagtgccct tcttgttagg gttcacagag tactttcgca
     5701 gagcattacc cggcttaagc acctttgaag cttgctcatt agcggcatct gagaaggtgc
     5761 tctcaccatt tttaacgcta acgctcttag agcacgttac acgggctttt agcgcttcat
     5821 ctgataactc acttgccgaa tagccaaaaa gctctttaca ggtcagcata gggcttccaa
     5881 ggctcgttgt agtgtttccg gtgttctttt tactggtagc accgccatca ttcgtggtag
     5941 cggtctttcc agtagcagcc gaaacatcag tctgacctac gattgaacga aactctacac
     6001 cttggaaggt catctgaata cgaagaccgt tagc
//
LOCUS       DAJVXP010000190         5973 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVXP010000190 DAJVXP010000000
VERSION     DAJVXP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.190"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..202)
                     /locus_tag="OQS10_003836"
     CDS             complement(<1..202)
                     /locus_tag="OQS10_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS2294740.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
     gene            718..1287
                     /locus_tag="OQS10_003837"
     CDS             718..1287
                     /locus_tag="OQS10_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2294741.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2015..2188
                     /locus_tag="OQS10_003838"
     CDS             2015..2188
                     /locus_tag="OQS10_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS2294742.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OQS10_003839"
     CDS             complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OQS10_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS2294743.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OQS10_003840"
     CDS             complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OQS10_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS2294744.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4300..4890)
                     /locus_tag="OQS10_003841"
     CDS             complement(4300..4890)
                     /locus_tag="OQS10_003841"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS2294745.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OQS10_003842"
     CDS             complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OQS10_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS2294746.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5708..>5973)
                     /locus_tag="OQS10_003843"
     CDS             complement(5708..>5973)
                     /locus_tag="OQS10_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS2294747.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1605 a         1537 c         1447 g         1384 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
      301 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
      361 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc ccctcttcaa
      421 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
      481 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      541 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      601 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      661 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      721 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      781 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      841 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      901 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      961 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     1021 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     1081 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     1141 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     1201 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     1261 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
     1321 atgggggctt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
     1381 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     1441 cctgaaaagg ggcttcacag attctctata tatttcaata agttaggata aaaagggact
     1501 ataccgatac tgcatagaat ctgtgtagga ttgcatagtc ataggggttg gaacaagccc
     1561 aactacacct gataaagcct gttattaaca ggtagttgaa tgtacgtgtt tagctatgta
     1621 aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat tccgtagcga
     1681 ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt cacaaccact
     1741 tatttgattt ataacaactt tcacaagcac gtaattttgt cgcaaaatga cacattttta
     1801 tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga gatgatatct
     1861 attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtctacccg cgtcagttca
     1921 ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt taattctatg
     1981 caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag gcggttccgg
     2041 caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg gacagcacag
     2101 cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa
     2161 gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg catttttgtc
     2221 tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg gttgatactg atgacaaatg
     2281 taagcttgcc tgatgcgcga tgcttatcag gcctaccaga agattgcaat atattgaatt
     2341 tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa caatgcgtac
     2401 gttgtcaaca atctgcaccg ccgggtaacc ccggcggttt tctgtttatg gctcctgatg
     2461 aacaacttct ggcggtggaa cgtcaaccaa ccttctactt aacaacgcat tgagtaaaat
     2521 cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa
     2581 atcacccgcg cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa
     2641 atcgacacgg ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac
     2701 aattgatgca ccaccaatca ccgcggccgg aatggtatgg atcagcgcgc caaatttcgg
     2761 cgaaaagccc aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac
     2821 ttttgtcacg gccatcacac cgatattttc agcataggtg gtgacgccgc taccgccgac
     2881 agagccggaa agcatcgttg ccagaccatc accgacgaat gctcgcccca tatacgggtc
     2941 catattgcgt ccggtcatcc cggcgaccgc cttgagatga cctaagtttt ccgccaccag
     3001 aatcaccgcc acgggcgcaa tcagcatcat tgcctgacta ttaaaagcag gagtggaaaa
     3061 atgtggcaaa ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag
     3121 ccctaaaacg ttggtcatca cgccatacag cagacaggcg acaattaaac ctacgagaat
     3181 caataaccgc tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt
     3241 cattaccgcc atccagctat caaaggctga agccgataca cttttcactg cgataggcgc
     3301 taagttcagg ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc
     3361 aatccagcgt gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca
     3421 ggcgataatc ccgcccagtg cgatgctgat attcgggtta atgccctgac cgttaaagcc
     3481 cgtcgcggca atcaccacgc cgacaaaagc cgcgctggag ccgagataac tggggacgcg
     3541 cccgccggta ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt
     3601 gggatccagc cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg
     3661 ttgaacgccc attactgccg tctgagcaaa cggcaatcgt tcatccggcg cgaccacgcc
     3721 gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg
     3781 tctccttaag gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac
     3841 accagcccgt agggtgtggt gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg
     3901 tgggtgccga cgctcaccac ctggctgatg cggcagtcaa acgaaaccag agcctcttcc
     3961 agttgcgggc atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg
     4021 ggcgttttgc cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca
     4081 cacagcgttc gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc
     4141 agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc
     4201 ccggatggac cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg
     4261 cgaaaggttt gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc
     4321 ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg ctatcgccat agccgatatt
     4381 aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtgttgacg
     4441 gtcaaaaccc gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata
     4501 ggccgcctgc atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt
     4561 aaaccaactg cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact
     4621 gtcccaggca acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga
     4681 aagcgccggg tgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg
     4741 agaacagtta gctgatgtcg gcccccattt tatcggggca taaagctccc gtaacgtctc
     4801 atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct
     4861 aagcgcacct gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcacggtg
     4921 atgtaacagg ctggcaagcc cgttgagtaa cagagcatta aacgtttcgg ggtcggtcac
     4981 gttgcaggcg tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg
     5041 aagctcactg gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac
     5101 cgggcagcgg atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg
     5161 acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag
     5221 gcgaggtgcg cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac
     5281 ccatgcctgc gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt
     5341 atgggcgttt attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata
     5401 atccagcgcc agctgcattc ccaccagcgc accgagcgca tggccgacca ccgcgtaatg
     5461 ctcaatccct gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta
     5521 atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg
     5581 atactcctgc tccagcaccg ccagttgcgg taaccagtaa ctgccgctac cgccaagacc
     5641 cgaaatcaac accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat
     5701 cgcggcctca cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta
     5761 ccagtccgca ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga
     5821 tttcgttaat cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat
     5881 ccgccatcgt gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg
     5941 tttgcgcctt tgggtcatcg gcaaacagca cgt
//
LOCUS       DAJVXP010000191         5961 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVXP010000191 DAJVXP010000000
VERSION     DAJVXP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.191"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            292..368
                     /locus_tag="OQS10_003844"
     tRNA            292..368
                     /locus_tag="OQS10_003844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="OQS10_003845"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="OQS10_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS2294748.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="OQS10_003846"
     CDS             complement(1357..2247)
                     /locus_tag="OQS10_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS2294749.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OQS10_003847"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OQS10_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCS2294750.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="OQS10_003848"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="OQS10_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAJVXP010000192         5836 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVXP010000192 DAJVXP010000000
VERSION     DAJVXP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.192"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..242)
                     /locus_tag="OQS10_003849"
     CDS             complement(<1..242)
                     /locus_tag="OQS10_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2294751.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OQS10_003850"
     CDS             complement(604..3387)
                     /locus_tag="OQS10_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS2294752.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OQS10_003851"
     CDS             complement(3444..5816)
                     /locus_tag="OQS10_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS2294753.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1488 a         1320 c         1311 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAJVXP010000193         5832 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVXP010000193 DAJVXP010000000
VERSION     DAJVXP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.193"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            122..952
                     /locus_tag="OQS10_003852"
     CDS             122..952
                     /locus_tag="OQS10_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCS2294754.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OQS10_003853"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OQS10_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS2294755.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="OQS10_003854"
     CDS             complement(2081..3541)
                     /locus_tag="OQS10_003854"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS2294756.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OQS10_003855"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OQS10_003855"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS2294757.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>5832
                     /locus_tag="OQS10_003856"
     CDS             5146..>5832
                     /locus_tag="OQS10_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCS2294758.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSI"
BASE COUNT         1396 a         1512 c         1465 g         1459 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctccttc
     5821 ggcgtttcga tt
//
LOCUS       DAJVXP010000194         5616 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVXP010000194 DAJVXP010000000
VERSION     DAJVXP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.194"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..513)
                     /locus_tag="OQS10_003857"
     CDS             complement(<1..513)
                     /locus_tag="OQS10_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS2294759.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OQS10_003858"
     CDS             complement(759..1211)
                     /locus_tag="OQS10_003858"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2294760.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQS10_003859"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OQS10_003859"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS2294761.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQS10_003860"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OQS10_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS2294762.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OQS10_003861"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OQS10_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OQS10_003862"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OQS10_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS2294763.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5616)
                     /locus_tag="OQS10_003863"
     CDS             complement(5494..>5616)
                     /locus_tag="OQS10_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294764.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1458 g         1351 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta cacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgat
//
LOCUS       DAJVXP010000195         5614 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVXP010000195 DAJVXP010000000
VERSION     DAJVXP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.195"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="OQS10_003864"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="OQS10_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS2294765.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="OQS10_003865"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="OQS10_003865"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS2294766.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="OQS10_003866"
     CDS             2475..4124
                     /locus_tag="OQS10_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS2294767.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="OQS10_003867"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="OQS10_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAJVXP010000196         5599 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVXP010000196 DAJVXP010000000
VERSION     DAJVXP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.196"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..150
                     /locus_tag="OQS10_003868"
     CDS             <1..150
                     /locus_tag="OQS10_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS2294768.1"
                     /translation="CYPISKVAKVCGYASVSYFTYVFRRYFGVSPSQYSQRSSESKIL
                     THQGI"
     gene            complement(192..644)
                     /locus_tag="OQS10_003869"
                     /pseudo
     CDS             complement(192..644)
                     /locus_tag="OQS10_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            708..854
                     /locus_tag="OQS10_003870"
                     /pseudo
     CDS             708..854
                     /locus_tag="OQS10_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(854..2011)
                     /gene="intS"
                     /locus_tag="OQS10_003871"
     CDS             complement(854..2011)
                     /gene="intS"
                     /locus_tag="OQS10_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS2294769.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2173..2247)
                     /locus_tag="OQS10_003872"
     tRNA            complement(2173..2247)
                     /locus_tag="OQS10_003872"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2212..2214),aa:Arg,seq:cct)
     gene            complement(2323..3255)
                     /locus_tag="OQS10_003873"
     CDS             complement(2323..3255)
                     /locus_tag="OQS10_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS2294770.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3549..4304
                     /gene="mlaA"
                     /locus_tag="OQS10_003874"
     CDS             3549..4304
                     /gene="mlaA"
                     /locus_tag="OQS10_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS2294771.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4486..4821)
                     /locus_tag="OQS10_003875"
     CDS             complement(4486..4821)
                     /locus_tag="OQS10_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294772.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(4779..5171)
                     /locus_tag="OQS10_003876"
     CDS             complement(4779..5171)
                     /locus_tag="OQS10_003876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294773.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5178..5554)
                     /locus_tag="OQS10_003877"
                     /pseudo
     CDS             complement(5178..5554)
                     /locus_tag="OQS10_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1367 a         1289 c         1243 g         1700 t
ORIGIN      
        1 tgttatccta ttagtaaagt agctaaggtc tgtggttatg ccagtgtctc atactttact
       61 tatgtattta gacgttattt tggtgtttct ccaagtcaat actctcagag gagttcagaa
      121 agtaaaattc ttactcacca gggaatctga tcattgttct tgccccctta tttccagaca
      181 gggggtgtat cttaagttaa cgttacccgc tgacgtcgat attctcgcgg agagcgataa
      241 cccagcgcac tgtgcggatg gttttcattg taatgttcga tcgccactgc aagattatgc
      301 aatgccgttc ttacattcgg tttcggcatg aacgcgatgt agtcttcctt catcgttttc
      361 acgaaccttt ctgccattcc attactctga ggactgctga ttgccgttgt gcagggctcc
      421 agattcaact ctctggcgaa ctgccgtgtt tcatgcgctc tatacgctga accgttatct
      481 gtcagccact ggatggactg ttccggtact ttatcgccaa agcgtttttc tatcgctcct
      541 aacatgacat cctgcactgt cgctttatcg taacctcccg tgcttgcggc ccagtctatg
      601 gcttcacgat cgcaacagtc cagggcgaac gtaacacgca gttttgaatc gccacgggtt
      661 taacagacac ctcagagtca tttaagatgg cttaaagaga ggtgcccatg agcggtaagc
      721 gttatcccga agagtttaaa actgaagcag tcaaacaggt tgttgatcgc ggttattctg
      781 ttgccagcgt tgcaacacgt ctcgatatca ctacccacag cctttatgcc tggataaaga
      841 agtacggtcc ggattactcc actttttcat caagccaatc cgcccaccac tgcatcattt
      901 ctctgcgctt atcgagatac tgagcatggt tgtaaatacc gcgcacagat ccgccgttgg
      961 catgtgccag ctgcacttca atagcgtcag caggccattc gtgctcgttc ataatcgtgc
     1021 tgaattcatg cctgaatccg tgaccgcttt ccagaccttc atagccgatt tgtttgatca
     1081 caagcaatac cgcgttctcg cagattggct tcttcttatc gttgcgcccg gcaaaaacaa
     1141 actctgagac tggtttggtg attgagctta gcgtagtgag aagttcaacc acctggtctg
     1201 acatcgggac cacatgaatt ttgcgtccct tcatcacact ggcgtcgatg gtgataatcc
     1261 tgttttcaaa atcgacgttc ttccatagca tggaacgaag ctcttttgtt cttagggctg
     1321 tgtagcgtaa aactttggtc gcaatgagcg atacgatact tcctgaaaat gttgccagtg
     1381 ctttgttgaa tgcaggcatc tggtctgctg gaaggaacgg gaagttcttc ttgcggtatc
     1441 ctttcatggc gtctgcaagg tcaggtgccg ggttatattt agcccttccg gtgacaatag
     1501 cgtaacgaaa aacctcgccg catcttctgc gggctttgtt ggctcgctcc attgcaccgc
     1561 gatcttcaaa tctgcggatt acttccagca gttgcatcgg ctcaatatcc tgaatctcaa
     1621 gaccgccgat gatgggtaaa atgtcgtcat caaacatttt ggcaagttca gttgcatagc
     1681 ctactgacca gacttgcttc ttgtgctcgt accattcctt gtaaatcgca ctaaatgaat
     1741 tgttgttaga cgaagccttt ttcgccttta ccggaacgat gccaaccgag atgtctttcc
     1801 tcgcagtcca tgccttatcc cttgcttcct gcaaagtcat aagcggatat tttccgacgg
     1861 tcaggatttt ctccttaccg tcaatcttgt agcgaagctg ccataccttt ttcccggata
     1921 caggaacata aaggtacagg ccattaccat cgagaaggcg gtatggtttt tctttcggct
     1981 ttgctgcttc aatctgctta acggtgagca tgggtaaaaa tccggtgggt aaaattattt
     2041 tatccacttt ttacccgtca tggagtgcgg ctgtcaacga tctgacgcga accatgacga
     2101 accgtgaata tacggaaggc ttgatattca ggggattttg cggactggta cggatgggag
     2161 cgaactgata aatggtgtcc cctgcaggaa tcgaacctgc aattagccct taggaggggc
     2221 tcgttatatc catttaacta agaggacaat gcggcatgag tatacccgct aatggagtgc
     2281 ggggtaagta cgctgccgct cgattgctta aaccctcgcc atttatgccg ggtttttatc
     2341 atttttctta atgttttccg cacgttctgc tttttggcgt gcttctgctt tacgcttgtt
     2401 gctcatgtcg ttacgaatct gtgcatgact cattaacgcg aagataaagg tgccgccgca
     2461 gatgttcccc gctaaagtag gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt
     2521 accgttaaac accagataga ggatttcaac agaaccgacc acgatatggg tggtgtcacc
     2581 tagggcaata agccaggtca tcaatataat caccacaatc tttgccgcac ctgctgcagg
     2641 aaacatccat accatagtgg cgatcagcca gccggaaatg atcgcgttgg caaacatctc
     2701 gctgggggtg ttcttcatca catccatgcc gattttgaca aatgcatcgc gagtttcttc
     2761 attgaagata ggcatatatt caaatgccca cgcagcaata ccagtcccga gaatattacc
     2821 cagcagcacg actccccata accgcataag taagccaacg ttgctcattg tcggtttttg
     2881 catgacgggt agtaccgcag tcacggtgtt ttcggtaaat aattgctggc gggccattat
     2941 gacgataata aaaccaaagg tataaccgag attctccagt aagaagctgc ccggcacacc
     3001 ttccagttcg acatgaaata tcccttttgc cagtaacgaa gcgcccatcg acagacccgc
     3061 cgcaatggct gaccacagta gcgccattgc gtcgcgttcc agctcttttt caccatcctg
     3121 gcggatatgc tcatgaattg ccatcgcccg ggaggggagt cggtcttcat ctatttctat
     3181 ttttttgccg cgctcttttt cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat
     3241 tttgtcgttg tccatggtct cttctcttga attagcacgt atagctaaag cgtagcggct
     3301 tttttgctcg caactggcgg gagttactct gaaaatgtag aaaaggctgc gtttgccttt
     3361 ttctgtttct atagaatcaa gtagcctaca gggcggcgat taccaggcta tgatcaaatc
     3421 agcaaatcag ggcgtctgga catcagttga cgtgctgtta caatcgccca cacctaaaca
     3481 ggcggatacg gtatcgttcc gtcatggatg gcaaactgca taagccataa aaaaacaggg
     3541 agacatttat gaagcttcgc ctgtcggcgc ttgctctggg aactacgctt ctggtggggt
     3601 gtgcgagttc cggtacagat cagcaagggc gttctgaccc gttagaaggg ttcaaccgca
     3661 ccatgtacaa cttcaacttc aacgtattag acccgtatat tgttcgaccg gtcgctgtcg
     3721 cctggcgtga ttatgttccg caaccggcgc gtaatggttt gagcaacttt actggcaacc
     3781 ttgaagaacc tgcggtgatg gttaactact tcttgcaggg cgacccttat caggggatgg
     3841 tccactttac ccgctttttc ctgaacacca ttttggggat gggcggcttt attgatgttg
     3901 cagggatggc gaacccgaaa ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg
     3961 gtcattatgg cgtgggttat gggccttacg ttcagttacc gttctacggt agcttcacgc
     4021 tgcgtgatga cggtggtgat atggcggatg gtctttaccc ggttctttcc tggctgacct
     4081 ggccgatgtc tgtgggtaaa tggacgcttg aagggatcga aacccgcgct cagctgctgg
     4141 attccgatgg tctgctgcgt cagtcgtccg atccttatat tatggtgcgc gaagcgtact
     4201 tccagcgtca tgatttcatc gctaatggcg gcgaactcaa accgcaggaa aatccgaacg
     4261 cacaagcgat tcaggatgat ttaaaagata ttgattctga ataagaaaca aataaaaaag
     4321 gtgagtcgca aaactcacct tttttgttgt tatccctcaa ttaccacaag taaaaggaga
     4381 taaaccgaca atgacccgga cgtaatgatg taaaaatact gatgttcgtc actgactatt
     4441 tccgatattc aggcgttgaa aaatcaaaaa actggaaaaa taattttaaa tatcatcaaa
     4501 attaacacct ggcgcagata gttgattaat ttcattttga tatttcgcct ggcgattttg
     4561 aagtcttttt tcaacgttat cttcgagatc cttaaagtct ttgtatttat tttcaggatg
     4621 gtcaacgtca actttgttag cgaataaatt agaaattatt tcttttacat tgtcgcgtct
     4681 aaattttgca tcgtccactt gaaaattata tcttattgct cgacctaaac gtttaaaaac
     4741 ttcgttaggt tctctcagtc tttccccgtt gagctcgttt acaacctgat gatcggaacc
     4801 tgttgcaaat gattctgcca tgatagcttt actgctttca aaccacgctg aagcattgct
     4861 gaagcattcc tggctttttc actacaatat tgggtgagac tatcaccatt aatacgaatt
     4921 cctctttgaa tgtggtagtc gtcctgatcg tagtaaccga gagataagta atcatttccg
     4981 tttttatttt taattacaat agcgagagag ttgccttttt tatctttacc gtaccaggta
     5041 aataagtccg ttggttcgct attcccgctc tcatcaaatt ctgttaccgt atgtctgagt
     5101 ttgtgtgata tcttatcgaa gataaaacca ggatctccat catatatttc ttttaatgcg
     5161 ctgaagatca tttccttgtt attgatatca tgagtcttct caactatgtt gtcattaatg
     5221 atagtacgga cgagttcttt gtctttatag ttcgcaggta attctattgg ttcttggttg
     5281 tcgataccaa tagtaatatg gtctgtatca ttggagccaa tatcaatata agtaactgag
     5341 gaattattaa tattaaatct taaggatcgt cgtttactcg tatggcgttt tttataagct
     5401 caagtagatg tttaatacag gtgcttattc cttccttacc attaaaaagg tttttgagct
     5461 cttgtactaa tccattaact tcatcattgt tattttcatt aatggattct gggtaagttg
     5521 cgctggtatt gttgattgaa atagatggta gcatgatttt attccatttt gattgatttg
     5581 ttacaaatat atattacct
//
LOCUS       DAJVXP010000197         5586 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVXP010000197 DAJVXP010000000
VERSION     DAJVXP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.197"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            547..894
                     /locus_tag="OQS10_003878"
     CDS             547..894
                     /locus_tag="OQS10_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS2294774.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            947..1342
                     /locus_tag="OQS10_003879"
     CDS             947..1342
                     /locus_tag="OQS10_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS2294775.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1383..2126
                     /gene="cmoA"
                     /locus_tag="OQS10_003880"
     CDS             1383..2126
                     /gene="cmoA"
                     /locus_tag="OQS10_003880"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS2294776.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2123..3094
                     /gene="cmoB"
                     /locus_tag="OQS10_003881"
     CDS             2123..3094
                     /gene="cmoB"
                     /locus_tag="OQS10_003881"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS2294777.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3259..>5586)
                     /gene="torZ"
                     /locus_tag="OQS10_003882"
     CDS             complement(3259..>5586)
                     /gene="torZ"
                     /locus_tag="OQS10_003882"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS2294778.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1291 a         1425 c         1341 g         1529 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggacttcct tatgagactg gttaatagag cgctatgaag
      121 attcaggagg gtaactataa ccatcacttt ctaactttca cgtaggaaat cgttaagttt
      181 cttgatagtt acaccatgtg accaactatc tatatatcgt tatatagccc tgtatatagc
      241 actatatgta gtgtctggag agggacgact aacacgatac agtttaccct gaaaagagac
      301 ttcacagatt ctttatatat ttcaataagt tatgctaaaa agagactata ccgatactgc
      361 atagaatttg tgtaggattg catagatact tggaaaaaca ccgttaaatt gcatagtcat
      421 tcacctgata ttgtcctgtt tttacaggta gttgattcgg atgttctagg aatcatcctc
      481 atagttgatt cggttagttg ggcttgtacc aacccctaat caacctcata gtgtatttag
      541 accttgatga ttttagacag ccgccctgtt catgcagcac gtccacacag tgaagctatt
      601 cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat
      661 ccgctgattc gttttatcgg tagtgatgat atgacgcaaa accgggaatt atttcaggtc
      721 tggttacaga aattagcgca gtggcatcag accactacgc cttatctttt tttacatacg
      781 ccagacattg cccaggcccc ggaactggta cataccctgt gggaagactt acgtaaaacg
      841 cttccagaga tcggagcagt tccggctatt ccacagcaat cttctctttt ctgaatttgc
      901 cacctatcat agacaggtgc catcggccat tttaaaggga gtttgtatgg taagcgcgct
      961 atatgccgtt ttaagtgcgt tgttattaat gaagttctct tttgatgtcg ttcgcctgcg
     1021 aatgcagtac cgcgttgcct atggtgacgg tggttttagc gaactgcaaa gcgctattcg
     1081 cattcatggt aacgcggtgg aatatattcc catcgcgatt gtgctgatgc tgtttatgga
     1141 aatgaatggc gcagaaacct ggatggtgca tatttgcggc atcgttttgc ttgctggtcg
     1201 tctgatgcat tattacggtt ttcatcaccg tctgtttcgc tggcgacgtt ccggcatgag
     1261 cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat ctttggtata tgccctggga
     1321 gttggttttc tccctgcgtt agcgcacaat acgccacttt ctttttcccg gatttttacg
     1381 ttatgtctca ccgcgacacg ctattttctg cccctatcgc cagactgggc gactggacct
     1441 ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt cccggctatt
     1501 ccaatattat ttccatgatt ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg
     1561 tttacgatct gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc
     1621 atgataattg caaaattatt gccatcgaca actccccggc gatgattgaa cgctgcagtc
     1681 gtcatattga cgcctataaa gcccctacgc cagtagacgt cattgaaggt gatattcgcg
     1741 atatcgccat tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa ttcctggaac
     1801 cttccgagcg ccaggcatta ctggataaaa tttatcaagg actgaacccg ggcggcgcgc
     1861 tggtgctttc ggaaaaattc agtttcgaag atgccaaagt tggtgaactg ttgttcaaca
     1921 tgcaccacga ctttaaacgt gccaacggtt acagcgaact ggagatcagc cagaagcgca
     1981 gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca cgcctgcata
     2041 aagccggttt tgagcatagc gagctgtggt tccagtgctt taactttggt tcactggtgg
     2101 cattaaaagc agaggacgct gcatgatcga ctttggtaac ttttattctc tgattgccaa
     2161 aaatcatctt tcacactggc tcgaaacgct gcccgcgcag attgctaact ggcagcgcga
     2221 gcagcagcac gggctgttta agcagtggtc caacgcggtg gaatttctgc ctgaaattaa
     2281 accgtatcgt ctggatttat tgcatagcgt aaccgccgaa agcgaagagc cactgagcac
     2341 cgggcaaatt aaacgcattg aaacgcttat gcggaacctg atgccatggc gcaaagggcc
     2401 gttctcactg tatggcgtca acatcgatac cgaatggcgt tccgactgga aatgggatcg
     2461 tgttctgccc catctttctg atttaaccgg acgcaccatt ctggatgtcg gctgcggcag
     2521 cggttatcac atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc
     2581 tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta acgatcagcg
     2641 cgcacatttg ttaccgttag gtattgaaca gcttccggca ctgaaagcct ttgataccgt
     2701 cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg gagcatctct ggcagttgaa
     2761 agatcaactg gtgaatgagg gtgaactggt gctggaaacg ctggttattg atggcgatga
     2821 gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc
     2881 ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt tttgtagata tccgcattgt
     2941 ggatgtgtgc gttaccacta ctgaagagca gcgacgcact gaatggatgg ttaccgaatc
     3001 tctgtccgat ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt atcctgcgcc
     3061 taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg
     3121 cgtgaatgcc ttatccggcc tacaaaccct tgctaattca atctattgca gggactatgt
     3181 aggcctgata agcatagcgc atcaggcagt tttacgtttg cataacctca gcgcccgttt
     3241 ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg ttaacttcgg
     3301 tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac aggcatttgc
     3361 cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc
     3421 gttttccaga tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc
     3481 agtcaccacc gcgcctgtca aaatctgacc acgtttgttc cagacacgca ccagatcgcc
     3541 attcgcaata ctaaatcgag cagcatcttc ggtgtgaata gtgatcggtt cacgatcggc
     3601 aaccgcatat tttttacgca gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg
     3661 atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc
     3721 aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg ttttggagta
     3781 aatctcaatt ttgccacttg gcgtacccag cgcatttttc accggatcgg cgcggaaatc
     3841 accataacga acgtactgtt cgttcttctc gctgcggcgc atttcgatca gtttattttg
     3901 ctgccagaag gcattaaaca ttggcatagt gacgcgttgc gcacgggccc ctttctgagc
     3961 ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat agatctcttt
     4021 tccgccaggt ttgagtaatt ccgcaagatc ggcaaacaca tcaaaatcgt ttcgcgcttc
     4081 aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt aatcaccggt
     4141 catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat ctgcgtgttt
     4201 agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct gccatgcttt
     4261 aatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc accagatcat
     4321 tttgatattc ggatacgttt gctctttacc gttatgctga tatttgccgc caggattttc
     4381 cagcgcatcg acgatacggg caacaggaat cgccctcatt ccgccatcat cagcggcctc
     4441 gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa cacgtgtcgg
     4501 gttgccacca ttggagtaat gataagagaa accaaaaccg ccgcccggtg taccaatctg
     4561 ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat attgctggcg
     4621 ctgaattccc cagcctgcca tcagcattgt gcgattcgcc gccatcagtt cagcgagttt
     4681 gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc tcttcggcgt
     4741 attatcgctc ttacctctca gatactcttc aaactgcgga tacccggtag tgtatttttc
     4801 aaggaacact ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc ctaacatcag
     4861 tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcgccaa agaagtcgat
     4921 ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt tcagctgatg
     4981 gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg tattcagcgg
     5041 gttcatcccc cacagcacca caacctggct gttttccaga atcagcggcc aggaggtctg
     5101 ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg ccgccccggt
     5161 tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac gttgcagtaa
     5221 ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg caaaaatagc
     5281 cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt ttaatgcttg
     5341 ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt gcagtggatt
     5401 atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt gtacctgatc
     5461 cgccgccgta gactgtaagg aattcggtat ggttttcgcc agcgcgcctg ttgaagaaac
     5521 aatcttgccg tccttcactt ctacgttcat cgctccccaa cgttccgcgg tgaggatttt
     5581 accgtc
//
LOCUS       DAJVXP010000198         5578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVXP010000198 DAJVXP010000000
VERSION     DAJVXP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.198"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..728
                     /locus_tag="OQS10_003883"
     CDS             <1..728
                     /locus_tag="OQS10_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2294779.1"
                     /translation="GTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEV
                     LNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGL
                     VRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTK
                     KDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWN
                     CAASKSPPKTPDEKQDTPDMREY"
     gene            796..4338
                     /locus_tag="OQS10_003884"
                     /pseudo
     CDS             796..4338
                     /locus_tag="OQS10_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5319..5578
                     /locus_tag="OQS10_003885"
                     /pseudo
     CDS             5319..5578
                     /locus_tag="OQS10_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1372 a         1360 c         1749 g         1097 t
ORIGIN      
        1 cggggacgaa gacgcagatg accattcgtt cgaaaaccta taagggcagc gggtttaatg
       61 aactgaagtt tgacgatgcg acagggaaag aacaggtcta catccacgcg cagaagaaca
      121 tgaacaccga ggtgctgaat aaccgcacca cggatgtgat aaacaaccat gctgaaacca
      181 ttggcaacaa tcagatgatt gcggttacca acaatcagat agagacggtg ggcgttaacc
      241 agatagagac ggtgggcagt aaccagataa ttaaagtggg ttctgttcag gtggaaacga
      301 ttggacttgt tcgtgcgctg acggtgggcg tggcgtatca gacgacggta ggtggcatta
      361 tgaacacctc ggtggcactg atgcagtcct cgcagatcgg tttgcataaa tcgttgaggg
      421 tcgggctggg ttatgacgtc aaagtcggaa ataacgttac cttcaccgtt ggtaaaacga
      481 aaaaggatga taccgggcag accgcgattt actccgccgg tgagcatctg gagctctgct
      541 gtggtaaggc aaggctggtg ctgacgaagg acggacaaat ttttctcaac ggcacaaaaa
      601 ttcatttgca gggtaaggag caggttaatg gtgactcact gttgattaac tggaactgtg
      661 cagcctcgaa atccccaccg aagacccctg atgaaaagca ggatacgccg gatatgagag
      721 agtactgatc ccgtacctgt tcggtattca gtaacaaacc agcattaaaa ataaacagaa
      781 gaaggaattt gaatcatgag cggaaaacca gcggcgcgtc agggagatat gactcagtat
      841 ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg gcgcgcccac cggcgtggcg
      901 tgctcggtgt gtccgggcgg gatgacttcg ggcaacccgg taaatccgct gctgggggcg
      961 aaggtgctgc ccggcgagac ggaccttgcg ctgcccggcc cgctgccgtt cattctctcc
     1021 cgcacctaca gcagctaccg gacgaagacg cctgcaccgg tgggcgtttt cggccccggc
     1081 tggaaagcgc cttctgatat ccgcttacag ctacgtgatg acggactgat actcaacgac
     1141 aacggcgggc ggagcattca ctttgagccg ctgctgccgg gggaggcggt gtacagccgc
     1201 agtgagtcaa tgtggctggt gcgcggtggt aaggcagcac agccggacgg ccatacgctg
     1261 gcgcggctgt ggggggcgct gcgccggata tccggttaag cccgcatctt tacctggcga
     1321 ccaacagcgc acaggggccg tggtggatac tggggggggc tgagcgggtg ccgggtgctg
     1381 aggacgtact gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggacgctg
     1441 acgtaccggc gtgaggccgc cggtgacctg gccggggaaa tcaccgacgt gacggacggt
     1501 gccgggcggg agttccgtct ggtgctgacc acgcaggcgc agcgtgcgga agaggcccgc
     1561 acctcttcgc tatcttcttc tgacagttcc cgccctctct cagcctcagc gttccccgac
     1621 acactgcccg gtaccgaata cggccccgac aggggtatcc gcctttcggc ggtgtggttg
     1681 atgcacgacc cggcataccc ggagagcctg cccgctgcgc cactggtgcg gtacacgtat
     1741 acggaagccg gtgaactgct ggcggtatat gaccgcagca atacgcaggt gcgcgctttc
     1801 acgtatgacg cgcagcgccc gggccggatg gtggcgcacc gttacgcggg aaggccggag
     1861 atgcgctacc gctacgacga tacggggcgg gtggtggagc aactgaaccc ggcagggtta
     1921 agctaccgct atctttatga gcaggaccgc atcaccgtca ccgacagcct gaaccggcgt
     1981 gaggtgctgc atacagaagg cggggccggg ctgaaacggg tggtgaaaaa agaactggcg
     2041 gacggcagcg tcacgcgcag cgggtatgac gcggcaggaa ggctcacggc gcagacggac
     2101 gcggcgggac ggcggacaga gtacggtctg aatgtggtgt ccggcgatat cacggacatc
     2161 accacaccgg acgggcggga gacgaaattt tactataacg acgggaacca gctgacggcg
     2221 gtggtgtacc cggacgggct ggagagccgc cgggaatatg atgaaccggg caggctggta
     2281 tcggagacat cgcgcagcgg ggagacagta cgctaccgct acgatgacgc gcacagtgag
     2341 ttaccggcga cgacaacgga tgcgacgggc agcacccggc agatgacgtg gagccgctac
     2401 gggcagttgc tggcgttcac tgactgctcg ggctaccaga cccgctatga atacgaccgc
     2461 ttcggccaga tgacggcggt tcaccgcgag gaaggtatca gcctttaccg ccactatgac
     2521 aaccgtggcc ggttaacctc ggtgaaagac gcacagggcc gtgaaacgca gtatgagtac
     2581 aacgccgcag gcgacctgac tgccgttatc accccggacg gcaaccggag cgagacacag
     2641 tacgatgcgt ggggaaaggc ggtcagcacc acgcagggcg ggctgacgcg cagtatggag
     2701 tacgatgctg ccggacgtgt catcagtctg actaacgaga acggcagtca cagcgacttc
     2761 agctacgatg cgctggaccg gctggtacag cagggcggct ttgacgggcg gacgcaacgt
     2821 tatcattatg acctgaccgg aaaactcacg cagagtgagg atgaggggct tgtcaccctc
     2881 tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcga cccggcagag
     2941 cagtggcagt atgacggcca cggctggctg agagaaatca gccacctgag cgaaggccac
     3001 cgtgtcgctg tccactacgg ctacgacgat aagggccgcc tgacgggcga acggcagacg
     3061 gtggagaacc cggagacggg ggaactgctg tggcagcatg agacgaaaca cgcctacaac
     3121 gaacaggggc tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga gtggctgacc
     3181 tacggcagcg gttacctggc gggcatgaag ctgggcggga cgccgctggt cgagtatacg
     3241 cgggacaggc tgcaccgtga gacggtgcgc accctcgcac cggacctgga catccgcatc
     3301 ccgtatgcca ccgacccggc gggaaaccgc ctgccggacc cggagctaca cccggacagc
     3361 acgctcacgg cgtggccgga taaccgcatc gcagaggatg cgcactatgt ttaccactat
     3421 gatgaatacg gcagactgac ggagaagacg gaccgcatcc cgacgggtgt gatacggacg
     3481 gacgacgagc ggacccacca ctacgacagc cagcaccgcc tggtgttcca cacgcggata
     3541 cagcatggcg agccactggt cgagagccgc tacctctacg acccgctggg acggcgaatg
     3601 gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa
     3661 ccggaggaga catggtacgg ctgggacggc gacaggctga cgacggtaca gaccgacacc
     3721 acacgtatcc agacggtata cgagccggga agcttcacgc cgctcatccg ggtcgagaca
     3781 gagaacggcg agcgggagaa agcgcagcgc cgcagcctgg cggagaagct gcagcaggaa
     3841 gggagcgagg acgggcacgg cgtggtattt ccggctgaac tggtgcggct gctggacaga
     3901 ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgggtgtg gcttgcgcag
     3961 tgcgggctga cggtggagca actggccaga caggtggagc cggaatacac accagtgcga
     4021 aacgttcatc tttaccactg cgaccatcgg ggccagccgc tggcgctcat cagcgaagac
     4081 ggcgatacgg tgtggagcgg ggcgtatgat gagtggggca accagctaaa tgaggagaac
     4141 ccgcatcatg tgtatcagcc ataccgtctg ccggggcagc agtatgatga ggagtcgggg
     4201 ctgtactaca atcgtcaccg gtactatgat ccgttgcaga gacggtacat cacccaggac
     4261 cccattgggc tgaaaggtgg gtggaatctg tatcagtatc cgctgaatcc ggtgagcagg
     4321 atagatccgt tggggttatt ctcatgtgag agttgtagtc attttataga tgatataaat
     4381 aataaactgg ataaccttcc gtttaacccg acaaaaatgt ttacagctag tttaaatact
     4441 gctaattcag caaggctgta tgcaagtggc atgacaaaat tcggtataag tgttgctgcc
     4501 gatggaacga tcattgctgc cccagtcggt gcagcttcta tggctttagg ggcgtggaat
     4561 ataaaatctg cgacagctgc atataacaga gcaaatttac aattatcaga atcacttcag
     4621 gaaaatggta gagataggtg ttggagaaat ttattgggta ttttacctta tggtgagcag
     4681 tttgatgacc ctggtgaacc atcaatatta gacgtttatg gtgaaaagat tgagaatatc
     4741 aaagattctc caatgaagtt ttttgaagag cttgggacgc tggggttgta atgtgtattt
     4801 ttaatgatat ttttatgttt gtgtttattg ttagtagtat acttgtaacc gtgcattttg
     4861 tgtattcttt attttttaaa agtggaaagg taaaagataa ggtaaaaaaa tggattaaag
     4921 taatgtgtga tttattttgg gggataggat agtggtgtga tatggataaa tataaattac
     4981 agtggctggc atatttcgac ctgacgccgg agtagataaa aaaacagaca ggattggagt
     5041 atagcaagcg tctgacaatc tctcgggaca gtagtatgat gaagaggcgg ggttgtacta
     5101 taaccgtaac cggtactacg atccgctaaa gggcaagtat atgactcagg gaaagatcat
     5161 cagaagttca ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc
     5221 tcattacctg aagctgtttt tttattgctt atacataatc aaatactcct tacataatta
     5281 aggagaacaa aatggaaatt aaaaaattga tggaacattt tttgaagcaa tatggtgatt
     5341 ttgaaaatgg cattcctgtt cacgatacca ttgccagagt tgtatcctgt atcagtcctg
     5401 caaaatttca tgagtgcttt attaactgga tgcgtgactg ccattcttca gatgataaag
     5461 acgtcattgc aattgatgga aaaacgctcc ggcactctta tgataagagt cgtcgcaggg
     5521 gagcgattca tgtcattagt gcgttctcaa caatgtacag tctggtcctc gggcagat
//
LOCUS       DAJVXP010000199         5427 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVXP010000199 DAJVXP010000000
VERSION     DAJVXP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.200"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..557)
                     /gene="bamD"
                     /locus_tag="OQS10_003886"
     CDS             complement(<1..557)
                     /gene="bamD"
                     /locus_tag="OQS10_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS2294780.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYT"
     gene            692..1672
                     /gene="rluD"
                     /locus_tag="OQS10_003887"
     CDS             692..1672
                     /gene="rluD"
                     /locus_tag="OQS10_003887"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS2294781.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1669..2400
                     /gene="pgeF"
                     /locus_tag="OQS10_003888"
     CDS             1669..2400
                     /gene="pgeF"
                     /locus_tag="OQS10_003888"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS2294782.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2530..5103
                     /gene="clpB"
                     /locus_tag="OQS10_003889"
     CDS             2530..5103
                     /gene="clpB"
                     /locus_tag="OQS10_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS2294783.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1366 a         1295 c         1475 g         1291 t
ORIGIN      
        1 gtatagtact cggcaacgga gtattcatat ttcgccagac gatctttcag gaataccaga
       61 cgtttggtgg catcggtagt gtactgactg ttcggatagc cgcgcaccag tttggaaaag
      121 tcactaaacg cagctcgtgc atgttgagga tcgcgatcgc tacggtcaac gccaaagaac
      181 ccttgcagcg cactgtcatc cagcgccata ttggtcaggc cacgcatgta catgacataa
      241 tcgatattcg gatgggtcgg gttaaggcga ataaaacgat cgatggcagc ctgtgctaac
      301 ggcaaatcgg cgtttttata gtaggcgtag atgagatcca gctgcacctg ctgcgaatac
      361 ggaccaaacg gatagcgatt atctaacgct tccagttgcg ttattgcctg tctccagtta
      421 ccgtcctgca gcttttgttg tgcagtcgcg taaatttcat ttggcggatt atcaggtact
      481 tcttcctttg accccgagca acccgccaaa aacaggctta gtgtggcggc tgccaccaga
      541 tatttcatgc gcgtcatgac gttttgactt tcctcaaaat gtaatacggg agattctctg
      601 ttcctgctcc cggttaagac cagctacaat agcacactat attaaacggc aaagccgtaa
      661 aaccccaacg ataaacgaag aagcagtata tatggcacaa cgagtacagc tcactgcaac
      721 ggtgtccgaa aaccaactcg gtcaacgctt agatcaggct ttggccgaaa tgttcccgga
      781 ttattcacgt tcgcggataa aagaatggat cctcgaccag cgcgtgctgg ttaacggcaa
      841 agtttgtgat aagccgaaag aaaaagtatt gggtggcgag caggttgcca tcaacgctga
      901 gattgaagaa gaagcgcgtt ttgaaccgca ggatatcccg ctggatatcg tctatgaaga
      961 tgaagatatc attgtcatta ataaaccgcg cgacctagta gtacatcctg gcgcgggtaa
     1021 cccggatggc acggtactga atgcgttgct tcattattat ccgcccattg ccgatgtacc
     1081 gcgtgcgggc atcgtccatc gtctggataa agacaccacc ggcctgatgg ttgtggcaaa
     1141 aaccgttccg gctcagacgc gtttagtcga atctttgcaa cggcgtgaaa ttactcgtga
     1201 gtatgaagcg gtggcgattg gtcatatgac cgctggcggt acagtagatg aacccatcag
     1261 ccgccacccg accaaacgta ctcacatggc ggtgcatccg atgggcaaac cggcggtgac
     1321 tcactatcgc atcatggaac acttccgtgt gcacacgcgt ctgcggttgc gtctggaaac
     1381 tggacgtacg caccagatcc gcgtgcatat ggcccatatc actcatccgc tggtgggcga
     1441 tccggtttat ggtggccgtc cgcgtccgcc aaaaggtgct tcggaagcat ttatctccac
     1501 gctgcgtaag tttgaccgcc aggcgcttca tgcaaccatg ctgcgtcttt atcacccgat
     1561 ctctggcatc gaaatggaat ggcatgcgcc tattccacaa gatatggtgg agctgattga
     1621 ggtgatgcgc gccgatttcg aagagcataa ggatgaagtg gactggttat gagtaagctg
     1681 attgtcccgc agtggccact gccaaaaggt gttgcggcct gtagctccac tcgtatcggc
     1741 ggcgtgagct tgcccccgta tgactcactc aacctcggtg cccattgtgg cgataacccg
     1801 gatcacgttg aggagaatcg taagcgactt tttgctgcgg gcaatttgcc ttctaaaccg
     1861 gtctggcttg agcaggtaca cggcaaagat gtgcttaagc ttactggcga accttatgcc
     1921 tcaaaacgag cagatgcctc ttatagcaat acgcctggca cggtttgcgc agtgatgact
     1981 gccgactgcc tccctgtgct gttttgcaat cgagcgggaa cggaagtcgc cgccgttcat
     2041 gctggctggc gtggactgtg cgcaggcgtg ctggaagaga cggtttcctg ttttgctgat
     2101 aaacctgaaa acattctcgc ctggttaggg ccggcaattg gtccacgcgc gttcgaagtg
     2161 ggggcggagg ttcgcgaggc gtttatggca gtagacgcta aagcaagtgc agctttcatt
     2221 cagcatggtg ataagtatct ggcggatatt tatcagcttg cccggcagcg tctggcgaac
     2281 gtgggtgttg agcaaatttt cggcggcgac cgttgtacat atacggaaaa tgagactttc
     2341 ttctcttatc gtcgcgacaa gaccaccggt cgtatggcaa gtttcatttg gctgatataa
     2401 cctaaagaat caagacgatc cggtacgcgt gattttcttt tcacattaat ctggtcaata
     2461 accttgaata attgagggat gacctcattt aatctccagt agcaactttg atccgttatg
     2521 ggaggagtta tgcgtctgga tcgtcttact aataaattcc agcttgctct tgccgatgcc
     2581 caatcacttg cactcgggca cgacaaccaa ttcatcgaac cacttcattt aatgagcgcc
     2641 ctgctgaatc aggaaggggg ttcggttagt cctttattaa catccgctgg cataaatgct
     2701 ggccagttgc gcacagatat caatcaggca ttaaatcgtt taccgcaggt tgaaggtact
     2761 ggtggtgatg tccagccatc acaggatctg gtgcgcgttc ttaatctttg cgacaagctg
     2821 gcgcaaaaac gaggtgataa ctttatctcg tcagaactgt tcgttctggc ggcacttgag
     2881 tctcgcggca cgctggccga catcctgaaa gcagcagggg cgaccaccgc caacattact
     2941 caagcgattg aacaaatgcg tggaggtgaa agcgtgaacg atcaaggtgc tgaagaccaa
     3001 cgtcaggctt tgaaaaaata taccatcgac cttaccgaac gagccgaaca gggcaaactc
     3061 gatccggtga ttggtcgtga tgaagaaatt cgccgtacca ttcaggtgct gcaacgtcgt
     3121 actaaaaata acccggtact gattggtgaa cccggcgtcg gtaaaactgc catcgttgaa
     3181 ggtctggcgc agcgtattat caacggcgaa gtgccggaag gattgaaagg ccgccgcgta
     3241 ctggcgctgg atatgggcgc gctggtggct ggggcgaaat atcgcggtga gtttgaagaa
     3301 cgtttaaaag gcgtgcttaa cgatcttgcc aaacaggaag gcaacgtcat cctgtttatc
     3361 gacgaattac ataccatggt cggcgcgggt aaagccgatg gcgcaatgga cgccggaaac
     3421 atgctgaaac cggcgctggc gcgtggtgaa ttgcactgcg taggtgccac gacgcttgac
     3481 gaatatcgcc agtacattga aaaagatgct gcgctggaac gtcgtttcca gaaagtgttt
     3541 gttgccgagc cttctgttga agataccatt gcgattctgc gtggcctgaa agaacgttac
     3601 gaattgcacc accatgtgca aattactgac ccggcaattg ttgcagcggc gacgttgtct
     3661 catcgctaca ttgctgaccg tcagctgccg gataaagcca tcgacctgat cgatgaagca
     3721 gcatccagca ttcgtatgca gattgactca aaaccagaag aactcgaccg actcgatcgt
     3781 cgtatcatcc agctcaaact ggaacaacag gcgttaatga aagagtctga tgaagccagt
     3841 aaaaaacgct tggatatgct caacgaagaa ctgagcgaca aagaacgtca gtactccgag
     3901 ttagaagaag agtggaaagc agagaaggca tcgctttctg gtacgcagac cattaaagcg
     3961 gaactggaac aggcgaaaat cgctattgaa caggctcgcc gtgtggggga cctggcgcgg
     4021 atgtctgaac tgcaatacgg caaaatcccg gaactggaaa agcaactgga agccgcaacg
     4081 cagctcgaag gcaaaactat gcgtctgttg cgtaataaag tgaccgacgc cgaaattgct
     4141 gaagtgctgg cgcgttggac ggggattccg gtttctcgca tgatggaaag cgagcgcgaa
     4201 aaactgctgc gtatggagca agaactgcac catcgcgtca ttggtcagaa cgaagcggtt
     4261 gatgcggtat ctaacgctat tcgccgtagc cgtgcggggt tggcggatcc aaatcgcccg
     4321 attggttcat tcctgttcct cggcccaacg ggtgtgggga aaactgagct ttgtaaggcg
     4381 ctggcgaact ttatgtttga tagcgacgag gcgatggtcc gtatcgatat gtccgagttt
     4441 atggagaaac actcggtgtc tcgtctggtt ggtgcgcctc cgggatatgt cggttatgaa
     4501 gaaggtggct acctgaccga agctgtgcgt cgtcgtccgt attccgtcat cctgctggat
     4561 gaagtggaaa aagcgcatcc ggatgtcttc aacattctgt tgcaggtact ggatgatggt
     4621 cgtctgactg acgggcaggg gagaacggtc gacttccgta atacggttgt cattatgacc
     4681 tctaacctcg gttccgattt gattcaggaa cgcttcggtg aactggatta tgcgcacatg
     4741 aaagagctgg tgctcggtgt ggtaagccat aacttccgtc cggaattcat taaccgtatt
     4801 gatgaagtgg tggtcttcca tccgctgggt gaacagcaca ttgcctcgat tgcgcagatt
     4861 cagttgaaac gtctgtacaa acgtctggaa gaacgtggtt atgaaatcca catttctgac
     4921 gaggcgctga aactgctgag cgagaacggt tacgatccag tctatggtgc acgtcctttg
     4981 aaacgtgcta tccagcagca gatcgaaaac ccgctggcac agcaaatact gtctggtgaa
     5041 ttggttccgg gtaaagtgat tcgcctggaa gttaatgaag accggattgt cgctgtccag
     5101 taatgataaa acgagccctt cggggctcgt ttttgtctat aagttagacg gaaaagacta
     5161 tatttaagat gttttgcctg aaaaatgagc gaacgataaa gtttttatat ttttcgcttg
     5221 tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc
     5281 cgccgggtcg gcggggttct cctgagaatt ccggcagaga aagcaaaaat aaatgcttga
     5341 ctctgtagcg ggaaagcgta ttatgcacac cccgcgccgc tgagaaaaag cgaagcggca
     5401 ctgctcttta acaatttatc agacaat
//
LOCUS       DAJVXP010000200         5347 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVXP010000200 DAJVXP010000000
VERSION     DAJVXP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.201"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..147
                     /locus_tag="OQS10_003890"
     CDS             <1..147
                     /locus_tag="OQS10_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2294784.1"
                     /translation="VCGYTVNADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMI
                     QATA"
     gene            complement(187..858)
                     /locus_tag="OQS10_003891"
     CDS             complement(187..858)
                     /locus_tag="OQS10_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS2294785.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(965..1198)
                     /gene="yedF"
                     /locus_tag="OQS10_003892"
     CDS             complement(965..1198)
                     /gene="yedF"
                     /locus_tag="OQS10_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS2294786.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1195..2400)
                     /gene="yedE"
                     /locus_tag="OQS10_003893"
     CDS             complement(1195..2400)
                     /gene="yedE"
                     /locus_tag="OQS10_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS2294787.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2587..3000
                     /locus_tag="OQS10_003894"
     CDS             2587..3000
                     /locus_tag="OQS10_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS2294788.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3034..4521)
                     /gene="amyA"
                     /locus_tag="OQS10_003895"
     CDS             complement(3034..4521)
                     /gene="amyA"
                     /locus_tag="OQS10_003895"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS2294789.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4599..4964)
                     /gene="fliT"
                     /locus_tag="OQS10_003896"
     CDS             complement(4599..4964)
                     /gene="fliT"
                     /locus_tag="OQS10_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS2294790.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(4964..>5347)
                     /gene="fliS"
                     /locus_tag="OQS10_003897"
     CDS             complement(4964..>5347)
                     /gene="fliS"
                     /locus_tag="OQS10_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS2294791.1"
                     /translation="YAQIGVESAVMSASQQQLVTMLFDGVLSALVRARLFMQDNNQQG
                     KGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLRNDVSAVEEV
                     EALMRNIADAWKESLLSPSLIQDPV"
BASE COUNT         1332 a         1427 c         1337 g         1251 t
ORIGIN      
        1 gtatgtggat atacagtgaa cgccgatgta aatggcgctc gtaacatttt agcggcgggg
       61 cacgccgttc ttgcctgtgg agagatggtg cagtcaggcc gcccgttgaa gcaggaaccc
      121 accgaaatga ttcaggcgac agcctgaacg tagcagggat cctcgtcctt cagggcgtgg
      181 aggatgtcaa caaacaggtt gaattaactc cgcgccctgg tttttgacat tacctaccgc
      241 gcgcgatacc gggtgccagg tgaactggtt tgctggcaca cagccgttgg tagcaatttc
      301 tgatgcttct ttaccgccaa tctcttgccg catccattcc tgtgcagctt ctggcgacag
      361 aaccagcggg cggcggtcat gaatgtctac cagaccctgg tctgctgcag cagtgacaat
      421 taaaaatcct tcggcttcgt caccacgctc aaatggtgtg ctacctatcg cggccataaa
      481 aataggttgt ccatcagcac gatagataaa aaaaggctgt tttttgtcgc cttctttttt
      541 ccactcaaac cagccatcgg caaaacagat tgcccgacca tgttgccaga gcggtttaaa
      601 catgcgactg gttgcggcag tttctacgcg ggcgttaatc agcggcggtt tatcccacca
      661 tccgggagca tatccccaga aaaccggatc cagatgaagg tgttcatcac gttcactgag
      721 cagaagaact ttggttcccg gcgcgacgtt gtatctgcca atgggttcgg gatcgtaggg
      781 aatatcgcgt tcaatatctt ccgcgagaag cgcaaggtaa tcttcacgcg tttgggattg
      841 ggcaaagcgt ccacacatag aaacctcctg agatcatact gaaaagaata ggagaggggg
      901 cgggtaaaat ctatgtgtca gaggcaacaa tgttcccgct gtgggccaca gcgggaatga
      961 gggattactt ctgaattaaa taacgaatgg tcggcccgtc ttgctgaata tccagcaccg
     1021 tatagccgtg attacgcgca tccagtggaa tattattgat cgactgtgga cagtcgctca
     1081 ccacttccag gatttcccct ttttttaact gcggcatcgc ctcaagggtt gcgactgccg
     1141 gataagggca gggttcaccc accatatcaa ggcggtaatc aggaacgata tttttcatgc
     1201 gatctcctta gcagtctgcg gtgccgcacg gcggaagaag cgtttttccc agccgataat
     1261 caacatcagc gcagtaaaca acagcaaata tgtcaccagc aggccaccca tcgggccaaa
     1321 ggttttcagc aggttgattt tgtcccagtc ggtggccagc gccggagcga aatcatccca
     1381 gtaatacgcc agaatcgtcg agccgatcac attgcccaga ccgacccacc agtagtgcac
     1441 ctggccttct accgcgcggt acatccagcc ggtttcgcag ccgcctgcca gcacgatgcc
     1501 aaaaccaaac agtaaaccac caattaccgc gtttggtccc gcccacatga ttttgggttc
     1561 aacgcctaac tgtacgtaac tgaagatccc gatggcactc accgccatac cgataatgat
     1621 tgctttcgcc atatgggtac gtccggtgat ccacatatcg cggaacgccg aagtaaagca
     1681 gatttgcgca cgttcaatca gtaaaccaaa gccgacgcca aacagcattg ccagccccag
     1741 tttgggttgg ttcatcgccg tgagcagcgc ccagcccagc atgccgaaaa agaccagcat
     1801 cccgagacga aaacgacgcc gcgcctgatc cggtttttgc gtcagcggtg aggcggcaga
     1861 aactttctgc attttcacgg gaatacggaa gatgggcagc agggtaaagc gcgcgccaaa
     1921 ccatgaacca atggcagtgg cgatggcaaa gaaccaggca tgcagcgaga actgaggaat
     1981 gccggtaaag aacgccgcca ggttacagcc cattgccaga cgcgcgccaa aaccggcgat
     2041 aatgccgcca ataatggcct gcataatgcg gatacggctg cgcggcatgc gcagtttgac
     2101 attgttggcc cacagcgctg cggcaaagca gccgccaaac atgccgagga tcatcatccc
     2161 gtcgatgcgg gttaatggcg atccttccag atggataatt ttaaaataac cccactcttc
     2221 agcatggacg ccaaacagct gcaggagctg accgccccaa cgggtaaatt cacccgtgac
     2281 agcccaaaag gtgccagtaa tgccaaaata gtaagtggag agaatacccg ccgcgatgac
     2341 cgcagggatg ggtgcccaga atttaatcaa ccaggcgtgt ttgaattgct gccatgacat
     2401 gaatgtagcc tctgaactca gaaggtttga aacaagaatc gcgaatcata catcatgcaa
     2461 atttattttc ggggtaataa ttcacaaaaa taatagttag atcaaatttc tctctgaacc
     2521 tggaaaggct ggcgacatca gggctttaat gccacaatcc tcttttcttc tcatgcagga
     2581 cgaatgatga aaaaactcgc aattgcaggt gcgctcatgt tgctggcggg ctgtgccgaa
     2641 gtcgaaaact ataacaatgt cgtgaaaacc cccgcgccgg actggctggc gggctactgg
     2701 cagaccaaag ggccgcaacg tgcgctggtc agtccggaag agataggcag cctgattgtc
     2761 actaaagagg gcgataccct ggattgccgt cagtggcagc gggtgattgc cgtgccgggc
     2821 aagttgacat tgatgtctga tgatctcact aacgttaccg taaaacgtga gctgtacgag
     2881 gttgagcgtg acggtaacac cattgagtac gatggtatga cgatggagcg tgtggatcgc
     2941 ccgacggcag agtgtgccgc cgcgctggat aaagcgccgt taccgacgcc actgccgtaa
     3001 tgaaatctcc ccggcttgcc gccggggatg aatttaaatc acctcttcga taacccacac
     3061 gctgacgctg ccgccgttgc aaaagaaggt cgcttcgccg ttttcgtcgg tcactacgct
     3121 ctcttgccga ttccccagaa aatcacgcca ggttttattg ccgtaattct ctcccagatg
     3181 aatggttttt tcgccatcat ccccgttcga catgaccacc acgcagccgg gatattcgtc
     3241 agtgccactg cggctaaagg caatgcagtt cggatggtcg aaaaataacg tctgtacacc
     3301 gtgggcgaaa cgctgacggg cgagaattaa ctcatcaagc tgttcgatta ttggcatatc
     3361 tatcggatag gtttgcccgt caccaccgac atcttcgtaa tgcgcaccgt agaggtccgg
     3421 atagaacacc gaaggaacgc cattttcccg caacaaaatt agagcatacg ccagcggttt
     3481 aaaccacggt tcgaccggcg cttcgagggc ttgcaacggt tgggtgtcgt gattggctac
     3541 gagggttacg gcgtggaaag gatcggcttc cactaatgta cccgtgaaaa tctgcgtcat
     3601 gtcgtagtcg cgtcccatgc gcgatgcttc atggaatttc atctgcagcg gcgcatcgaa
     3661 cagcatggtt ttgccttcca cctgatcaat atacgtttgc agtttatcga cttcatgcga
     3721 ccagtactct gccacaataa acagcggctt tggcgcaact tcctgtacgt gttcgatcca
     3781 ctctttataa aaccacgccg gaatatgttt aaccgcatca agacgaaaac cgtcgcattg
     3841 tgtttgttcc atcacccagc gcgcccagta tttaatctct tccgtcacgg catgattgcg
     3901 gaaatcgata ttctcaccca tcagataatc gaaattaccg agttcatcat caacctgatc
     3961 gttccagcct tcgccggtgt agtcgttaac aattttaaat atgccatctt catcaggatt
     4021 ttcgatatgg tcgataccgc taaaacattt gaagtcccag ataaactgcg agtattgccc
     4081 ggcacgggcg gggaaggtgt aacgcgtcca gccttcgcac tcaatgattt cttcatcaat
     4141 ttgcgtacgg tcatcagcat ttacacgctg cacgcgaata gcttcttttt catccgcccc
     4201 cattttgtgg ttgactacca cgtccagtag caccgcaatg tcattacgtt tcagagcgtc
     4261 aatggcggcc agcagttgtg ctttatcgcc atatttagta gggatgctgc ctttctgatc
     4321 aaactcacct aaatcaaata aatcatagga gtcgtagccg accgaatacc cgcccgatgc
     4381 gcctttatag gcgggcggca accagaccat attgatacca atatcattaa aaccgtcggc
     4441 gcgctcggcc agttcaggcc agagcttacc gccttccggg taataccagt gaaaacattg
     4501 taacagcgtg ggattacgca ttcttcgact ccattcaagg ggaacattag aagcgtagcc
     4561 gtaatcggat tattcgcgag ccatcggctc gttaagattc aaaagaggtt atcctgcgga
     4621 gccagcacaa agccgccctg atctccatag gcacttaaca ctgatttttg caccgatgac
     4681 tgaccgacca gtttcgccag ttcatccata cgaatctgta ataactgctt taccttgctt
     4741 tcgttgtcga gaatcaggcg cagcatcggg cggagctgct cctgcatcgt ggtggacggg
     4801 gcaacctctt cagtcagatg tgcaatctcc tgcaccgcat tcacatacgc catttcgctg
     4861 gcgatgagtt cgtcccattg ttcttccgtt gccaggcgta acatgagctg gcttttatcg
     4921 acgagttgtt gccaggcgaa atacaaatgc ggtgcatggt tcatcagact gggtcctgaa
     4981 tcaaagaagg ggagagtaaa gactctttcc aggcatcggc aatattgcgc attaatgctt
     5041 ccacttcttc gactgcggag acatcgttgc gtaaattggc ttgcagcaag cgcctgacca
     5101 tatagctata aagagcaatc aagttttggg ttagttcgtc tttgctctct tcatcaagac
     5161 tcacccgcag cccgttctca atgatgttga tcgcttttga caaagagacg cctttgcctt
     5221 gctgattgtt gtcctgcata aacaggcgcg ctctaaccag tgcgctcagc actccatcaa
     5281 atagcatggt gaccagctgt tgctggctgg cgctcattac ggcgctttcg acgccaattt
     5341 gtgcata
//
LOCUS       DAJVXP010000201         5114 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVXP010000201 DAJVXP010000000
VERSION     DAJVXP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.202"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..379
                     /locus_tag="OQS10_003898"
     CDS             <1..379
                     /locus_tag="OQS10_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294792.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OQS10_003899"
     CDS             complement(575..2125)
                     /locus_tag="OQS10_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS2294793.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OQS10_003900"
     CDS             2297..2743
                     /locus_tag="OQS10_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCS2294794.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OQS10_003901"
     CDS             complement(2937..3872)
                     /locus_tag="OQS10_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS2294795.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OQS10_003902"
     CDS             4384..>5114
                     /locus_tag="OQS10_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294796.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENTDEC"
BASE COUNT         1418 a         1244 c         1126 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 acagatgaat gcat
//
LOCUS       DAJVXP010000202         5076 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVXP010000202 DAJVXP010000000
VERSION     DAJVXP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.203"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="OQS10_003903"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="OQS10_003903"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCS2294797.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="OQS10_003904"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="OQS10_003904"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCS2294798.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="OQS10_003905"
     CDS             complement(1322..2986)
                     /locus_tag="OQS10_003905"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCS2294799.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /gene="mhpR"
                     /locus_tag="OQS10_003906"
                     /pseudo
     CDS             3028..3861
                     /gene="mhpR"
                     /locus_tag="OQS10_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="OQS10_003907"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="OQS10_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCS2294800.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
BASE COUNT         1168 a         1462 c         1379 g         1067 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacact
//
LOCUS       DAJVXP010000203         5045 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVXP010000203 DAJVXP010000000
VERSION     DAJVXP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.204"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..311)
                     /locus_tag="OQS10_003908"
     CDS             complement(<1..311)
                     /locus_tag="OQS10_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS2294801.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     T"
     gene            complement(461..826)
                     /locus_tag="OQS10_003909"
     CDS             complement(461..826)
                     /locus_tag="OQS10_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS2294802.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            1035..2105
                     /gene="aroF"
                     /locus_tag="OQS10_003910"
     CDS             1035..2105
                     /gene="aroF"
                     /locus_tag="OQS10_003910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS2294803.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            2116..3237
                     /gene="tyrA"
                     /locus_tag="OQS10_003911"
     CDS             2116..3237
                     /gene="tyrA"
                     /locus_tag="OQS10_003911"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS2294804.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3280..4440)
                     /gene="pheA"
                     /locus_tag="OQS10_003912"
     CDS             complement(3280..4440)
                     /gene="pheA"
                     /locus_tag="OQS10_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS2294805.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(4463..4588)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(4540..4587)
                     /gene="pheL"
                     /locus_tag="OQS10_003913"
     CDS             complement(4540..4587)
                     /gene="pheL"
                     /locus_tag="OQS10_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCS2294806.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            complement(4691..5032)
                     /gene="raiA"
                     /locus_tag="OQS10_003914"
     CDS             complement(4691..5032)
                     /gene="raiA"
                     /locus_tag="OQS10_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS2294807.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
BASE COUNT         1243 a         1147 c         1290 g         1365 t
ORIGIN      
        1 aatgtcggtg actcgttgcc aaaataaaaa ccattgcagc ctgaaatcat cgcctcttgt
       61 tgagtgtgaa tgatgacagg cagataaggc aaagatgtcg cggcattctc ttgtagggcg
      121 gtactaaaac gtgaagatga aaagatacat aactttggtg gccctgatag cgcaggccag
      181 cgggtgtaac tgacaatacc ggaaaccatc atacgcacgt ttttcgcaac gtcagaaggc
      241 tcctgagcga gtattggtgc tcccgtcagc aggagaatca gaagtaagaa cagtcggtga
      301 gaaaaacgca ttcagggatc cccaccatct tcgctcgaaa tgcatcgata ttttttcaga
      361 atgctcatta aaatatcatt ttttcatgcg atcgtcatca atgtgagaaa taaaacacaa
      421 tgagtaaaat cttatgttta atttatttgt ggggttttaa ttaatttaat cgtattgtgc
      481 tggcattttc atcagcccct ttttgccata cttcatgcag ttggctggca ctttcaacat
      541 tgttacagct ggcaggaaag cttttaccgg aaagtccccg ggcataagta aaatcggtct
      601 ggcaagtttt cttttgtcct tcggcatatc ctttaaggta tagaccgcga tccgcctgtg
      661 aatcgctaaa tgcatcgtca tcttttatgg cgctgcccga tatcgcatct tccataccga
      721 catcatacca gtcggtgctg gtcaaggttg gcgcgtgagt ataaggatca atctgacatc
      781 cgctaaccag taaagccaat aagggggcga taaacttttt catcatttct ttctcctttt
      841 tcaaagcata gcggattgtt tttaaaggag gtgtaaattt atttgtacga aataagcatc
      901 aaaagcgcga gcaaattgtt cgtgtaaaga aaaatgtacg aaatatggat tgaaaacttt
      961 actttatgtg ttatcgttac gtcatcctcg ctgaggatca actatcgcaa acgagcataa
     1021 acaggatcgc catcatgcaa aaagacgcgc tgaataacgt acatattacc gacgaacagg
     1081 ttttaatgac tccggaacaa ctgaaggccg cttttccatt gagcctgcaa caagaagccc
     1141 agattgctga ctcgcgtaaa accatttcag atattatcgc cgggcgcgat cctcgtctgc
     1201 tggtagtttg tggtccttgt tccattcatg atccggaaac tgctctggaa tatgctcgtc
     1261 gatttaaagc ccttgccgca gaggtcagcg atagcctcta tctggtaatg cgcgtctatt
     1321 ttgaaaaacc ccgtaccact gtcggctgga aagggttaat taacgatccc catatggatg
     1381 gctcttttga tgtagaagcc gggctgcaga tcgcgcgtaa attgctgctt gagctggtga
     1441 atatgggact gccactggcg acggaagcgt tagatccgaa tagcccgcaa tacctgggcg
     1501 atctgtttag ctggtcagca attggtgctc gtacaacgga atcgcaaact caccgtgaaa
     1561 tggcctccgg gctttccatg ccggttggtt ttaaaaacgg caccgacggc agtctggcaa
     1621 cagcaattaa cgctatgcgc gccgccgccc agccgcaccg ttttgttggc attaaccagg
     1681 cagggcaggt tgcgttgcta caaactcagg ggaatccgga cgggcatgtc atcctgcgcg
     1741 gtggtaaagc gccgaactat agccctgcgg atgttgcgca atgtgaaaaa gagatggaac
     1801 aggcgggact gcgcccgtct ctgatggtag attgcagcca cggtaattcc aataaagatt
     1861 atcgccgtca gcctgcggtg gcagaatccg tggttgctca aatcaaagat ggcaatcgct
     1921 caattattgg tctgatgatc gaaagtaata tccacgaggg caatcagtct tctgagcaac
     1981 cgcgcagtga aatgaaatac ggtgtatccg taaccgatgc ctgcattagc tgggaaatga
     2041 ccgatgcctt gctgcgtgaa attcatcagg atctgaacgg gcagctgacg gctcgcgtgg
     2101 cttaagaggt ttattatggt tgctgaattg accgcattac gcgatcaaat tgatgaagtc
     2161 gataaagcgc tgctgaattt attagcgaag cgtctggaac tggttgctga agtgggcgag
     2221 gtgaaaagcc gctttggact gcctatttat gttccggagc gcgaggcatc tatgttggcc
     2281 tcgcgtcgtg cagaggcgga agctctgggt gtaccgccag atctgattga ggatgttttg
     2341 cgtcgggtga tgcgtgaatc ttactccagt gaaaacgaca aaggatttaa aacactttgt
     2401 ccgtcactgc gtccggtggt tatcgtcggc ggtggcggtc agatgggacg cctgttcgag
     2461 aagatgctga cactctcggg ttatcaggtg cggattctgg agcaacatga ctgggatcga
     2521 gcggctgata ttgttgccga tgccggaatg gtgattgtta gtgtgccaat ccacgttact
     2581 gagcaagtta tcggcaaatt accgccttta ccgaaagatt gtattctggt tgatctggca
     2641 tcagtgaaaa atggaccatt acaggccatg ctggcggcgc acgatggccc ggtactgggg
     2701 ttacacccga tgttcggccc ggacagcggt agcctggcaa agcaagttgt ggtctggtgt
     2761 gatggacgta agccggaagc ataccaatgg tttctggagc aaattcaggt ctggggcgct
     2821 cggctgcatc gtattagcgc tgtcgagcac gatcagaata tggcgtttat tcaggctctg
     2881 cgccactttg ctacttttgc ttatgggctg catctggcag aagaaaatgt tcagcttgag
     2941 caacttctgg cgctctcttc gccgatttac cgccttgagc tggcgatggt cgggcgactg
     3001 tttgctcagg atccgcagct ttatgccgac attattatgt cgtcagagcg taatctggcg
     3061 ttaatcaaac gttactataa gcgtttcggc gaggcgattg agttgctgga gcagggcgat
     3121 aagcaggcgt ttattgacag tttccgcaag gtggagcact ggttcggcga ttacgcacag
     3181 cgttttcaga gtgaaagccg cgtgttattg cgtcaggcga atgacaaccg ccagtaataa
     3241 tccagtgccg gatgattcac atcatccggc accttttcat caggttggat caacaggcac
     3301 tacgttctca cttgggtaac agcccaatac cttcattgaa cgggtaattt cccctaactc
     3361 tttcaatgct ttttgcattt ccgctgattc aagattggcc tgaatatcca gataaaatat
     3421 ctcttcccat ggattaccgt gaatcgggcg tgattccaga cgggtcataa tcagattgtg
     3481 gttgcgcagc accagcaacg cttcaaccag cgcaccggct tgttgcccgg tcgccattaa
     3541 caacgtcgtt ttcgccggaa cctggtcaga catgttaatg gctttacgtg ccaacaccac
     3601 aaatcgggtg aagttttgtt gctgattcgc ttcaatacgc tccagtacct gcaaaccgta
     3661 caaagtgccg ccagcttcgc ttcccaacgc agcaacatgc ggtgattttg cctgtgcaac
     3721 cttttccatt gccgcagacg tactttcggt atattcaatc ttccagtgag gataacgatt
     3781 aaggaatttg ctgcattgct ggaatggctg cggatggctg tagaccgtat taatggtgga
     3841 taaatcagtc gtgccggaga ccaacaaaca atggtcgata gttaacgtca tctcgccaac
     3901 aatcgacaag ctggtatgtt gcagcagatc gtaaacgtcg tttatggcac cggagctggt
     3961 attttcaatc ggtacgacgg catagtcggc ctggccggtt tccacctgat taaaaatatc
     4021 ggcaaatttg gcgcagccac tttcaatgaa ttgctcaaag tgacgggcag catactggcg
     4081 cgccgcaaga tgggaataag aacctttggg gccgagaaaa gcgatgcgtg ctgagtgcgg
     4141 attaatttta ttgagatgtt gctggagcaa agcctgctga gttaatacgg aatcttcaat
     4201 gatgagctgg aacaggcgag taatgtaatg ggcgtccaga tggtgcgctt taccgagcgt
     4261 aattaatctt tccagtaaat cgcgttcacg atcaatatct cgtaccgggc gatgcgagag
     4321 cagtttggct tttcccacct cgacggccag ttcgcgccgc tctgccagta atgctaataa
     4381 tttttcatcc agcgcgctga ttttctctcg cagcgccagt aatggatttt ccgatgtcat
     4441 agtgttgcct tttttgttat caataaaaaa ggccccccgg tttgggaggc cttattgttc
     4501 gtcttcgcat tctgtttcac acgacgaaac gcctcccatt caggggaagg taaaaaagaa
     4561 tgcgaagaaa aacggtgtgt gtttcatgtt tgtttcctta agtgacttta gtacagtacc
     4621 cgtactgttt tcacgctgtc aacaaaaaac gcgcccgaag gcgcgttggc gatccactca
     4681 atataaagga ctactcttct tcaacttctt cgacgaagtt ggcgtctttc accgatgttg
     4741 cggcacgacg tgcttcgcct ttgtgctgca gtttattgag ctgccgttcc agcttgttga
     4801 tcaattcgtt aattgcagtg tacatatctt catgtttacc actggcaacc agaacgccgt
     4861 taggtgtatt gattgtggcg tcagcaacaa acccttgtgg ctctttggac agaatgatat
     4921 gtggattaat cagatgtgtt tgccattttt ccagtttggc gagacggtct gcgacatgtt
     4981 ggcggatggc cggagtaatt tccatttgtt tgctggtaat gttcattgtc ataaatttta
     5041 cctct
//
LOCUS       DAJVXP010000204         5003 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVXP010000204 DAJVXP010000000
VERSION     DAJVXP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.205"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..117
                     /locus_tag="OQS10_003915"
     CDS             <1..117
                     /locus_tag="OQS10_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS2294808.1"
                     /translation="SLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            122..511
                     /gene="csgE"
                     /locus_tag="OQS10_003916"
     CDS             122..511
                     /gene="csgE"
                     /locus_tag="OQS10_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS2294809.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            536..952
                     /gene="csgF"
                     /locus_tag="OQS10_003917"
     CDS             536..952
                     /gene="csgF"
                     /locus_tag="OQS10_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS2294810.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            979..1812
                     /gene="csgG"
                     /locus_tag="OQS10_003918"
     CDS             979..1812
                     /gene="csgG"
                     /locus_tag="OQS10_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCS2294811.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1877..2368)
                     /locus_tag="OQS10_003919"
     CDS             complement(1877..2368)
                     /locus_tag="OQS10_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS2294812.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2470..3024)
                     /gene="ycdY"
                     /locus_tag="OQS10_003920"
     CDS             complement(2470..3024)
                     /gene="ycdY"
                     /locus_tag="OQS10_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS2294813.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3048..3785)
                     /gene="ycdX"
                     /locus_tag="OQS10_003921"
     CDS             complement(3048..3785)
                     /gene="ycdX"
                     /locus_tag="OQS10_003921"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS2294814.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3840..4778)
                     /gene="ghrA"
                     /locus_tag="OQS10_003922"
     CDS             complement(3840..4778)
                     /gene="ghrA"
                     /locus_tag="OQS10_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS2294815.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1349 a         1259 c         1159 g         1236 t
ORIGIN      
        1 tcgttgttca tcagcgaaaa tacggttaaa acgcatcttt ataatctttt caagaagata
       61 gccgtaaaaa accggacaca agcggtttcc tgggcaaacg ataacctcag gcgataaagc
      121 catgaaacgt tatttacgct ggattgtggc ggcagaattt ctgttcgccg cagggaatct
      181 tcacgccgtt gaggtagaag tcccgggatt gctaactgac catactgttt catctattgg
      241 ccatgatttt taccgagcct ttagtgataa atgggaaagt gactatacgg gtaacttaac
      301 gattaatgaa aggcccagtg cacgatgggg aagctggatc actataacgg tcaatcagga
      361 cgttattttc cagacttttt tatttccgtt gaaaagagac ttcgagaaaa ctgtcgtctt
      421 tgcactgatt caaactgaag aagcactaaa tcgtcgccag ataaatcagg cgttattaag
      481 tacgggcgat ttggcgcatg atgaattcta aataaaaaat tgttcggagg ctgcaatgcg
      541 tgtcaaacat gcagtagttc tactcatgct tatttcgcca ttaagttggg ctggaaccat
      601 gactttccag ttccgtaatc caaactttgg tggtaaccca aataatggcg cttttttatt
      661 aaatagcgct caggcccaaa actcttataa agatccgagc tataacgatg actttggtat
      721 tgaaacaccc tcagcgttag ataactttac tcaggccatc cagtcacaaa ttttaggtgg
      781 gctactgtcg aatattaata ccggtaaacc gggccgcatg gtgaccaacg attatattgt
      841 tgatattgct aaccgcgatg gtcaattgca gttgaacgtg acagatcgta aaaccggaca
      901 aacctcgacc atccaggttt cgggtttaca aaataactca accgattttt aagccccagc
      961 ttcataagga aaataatcat gcagcgctta tttcttttgg ttgccgtcat gttactgagc
     1021 ggatgcttaa ccgccccgcc taaagaagcc gccagaccga cattaatgcc tcgagctcag
     1081 agctacaaag atttgaccca tctgccagcg ccgacgggta agatctttgt ttcggtatac
     1141 aacattcagg acgaaaccgg gcaatttaaa ccctacccgg caagtaactt ctccactgct
     1201 gttccgcaaa gcgccacggc aatgctggtc acggcattga aagattctcg ctggtttata
     1261 ccgctggagc gtcagagttt acaaaacctg cttaacgagc gcaagattat tcgtgcggca
     1321 caagaaaacg gcacagttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca
     1381 aatatcatgg ttgaaggttc gattatcggt tatgaaagca acgttaaatc gggcggtgtt
     1441 ggggcaagat attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg
     1501 aacctgcgtg tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag
     1561 acgatacttt cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg
     1621 ctggaagggg aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct
     1681 atcgaaacag gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg
     1741 cagaacaaag cagaacggca gaatgacatt ctggttaaat accgccatat gtcggttcca
     1801 ccagaatcct gacagataaa aatgccggaa ttaataatcc ggcattttac tgtttaagcg
     1861 aaagcgtgag tgcccctcac gctttatttt tgatttcctg ctcacggtga gccgtctttg
     1921 cactacgcgc cgccagccac aggccgctgt ttttcattgc gtaaccaaat accagcccaa
     1981 gcgccagcga aggtaacacc agtttccagt caccctgccc ggcaaacgtc gcgcatgcgc
     2041 ctataaacgt ccccggaaca aatgaaagca gcagctgttt ggcctgaata cacatcagaa
     2101 acgccacaat accggttatg acataaccga gaatttccag atgtggtgcc agtgcactac
     2161 cgtaaataat gaccatcgcc cacaccacgc cactaagcag ggttgcagcg gagatcgcca
     2221 gccctttcag cccaccttgc gggcaggcaa agtaggccgt acagcccaag aagcccgccc
     2281 agcttagtaa gcccagggaa acagccaccc atccccagat accggagaga atgcctgttg
     2341 tgattgcaat agagagaagt atgttcatgc cgcgcatgat agcagaaaac gcggccatga
     2401 atgagatcga gcacacattt tatgcaacaa caaattatct gttgcagtta tgagtgacga
     2461 tgatcacatt tactcttcag aatcttcttc cagctcgtcc cacattgcac taatggcatc
     2521 acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt gcatgggcct ccactttgcc
     2581 gaggaacgca ccacaccagg gtaacagata ctcactgaac agtgtttcca gtgcttcgct
     2641 ctcatcttcc gttgactgat cttccagcca ggaagccgca agcagcaatg tgccgatgtg
     2701 atcggctggc gtatccgcca gtggcatccc tcgttcggaa agaaaagcgc gcacttccgc
     2761 ttccgttacg tcctcaaccc atgcgctacg atatggcggc actgcacatt catcgccgat
     2821 aaacaacgca ttgtaatcgg cagagacttg cgccatatca cagcttttct gtaaacgcgt
     2881 cagtaactca tcctgctcca gtggccagtt cgcagccagt ttcccctcac gaatcagggt
     2941 aaacagcggc accagtaaag gatcttgcgg ttggcggtaa tagagcgaac ccagcacacg
     3001 acagaggata gaaaactcgt tcattaatat atttccatta ctagtaatta aaggtctgca
     3061 aattccgcaa tcggtgccat accgcgagat tcaaggaagt tcagtaagcg gcgcggagaa
     3121 acattcaaaa tgcgctctgg cggaaaatct accgcgtcga ggattttaag acactcttca
     3181 aattccccca tggtaaacgc ggtgtgagaa tccgagccta atgccaccca acctccagca
     3241 tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac tgcccttacg tgagtgtaaa
     3301 aatgaagaat tattgatttc cagcgccacc tgatgtttcg cggctgcttc agcaacggct
     3361 ttcacatcta tttcatattt gggatttccg ggatggctta ttatatgcac attgccgctg
     3421 gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt catgtggtgc aaaaaccggc
     3481 tcatgaaaac cggcaataat cagatccagc gagtcaaaca ttttaccgct gcaatcaatt
     3541 tcaccatcaa catttttaat gttagcttcg atgccgcgca ggatccctac cccatcaacc
     3601 actcgcggcc agatacgcat gttaatgaag tgccagtgat gcggagcatc ttccatatcc
     3661 gggccatgat cggtgatcgc aaaaagttta atgccctttt gtttggcctg ggcaatgtaa
     3721 tcactcaatg tgctatatgc atgtgtgctg gcaacggtat gcatatgaag gtcgacggga
     3781 tacataactc tctcctgtat tcaattcccg ccaaggatag caggaatcct gatgctttat
     3841 tagtagccgc gtgcgcggtc gacttgcccg cagaccctct cccctttttc gagctgggca
     3901 atggtgcgag aaatgtactc cacagcttca gcgggacggg taatcgcggc gacatgtggt
     3961 gttatcgtca cgcgtggatg ttgccagagc ggactttcag gcggtaaggg ttcacgatta
     4021 aaaacatcca acattgcgcc tttaactttg ccgctatcca gcgccgcgag caggtcatct
     4081 tccacaacat gaacaccacg cgccaggttg aggagatacg cgccatccgg taatttttcg
     4141 agtaattgtt gattaataat gccgacggtt tcaggggtat tcggtaacaa attaatcaat
     4201 acccgacatt ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct ttgcacgcca
     4261 ggccacgatt tacgggttcg actccagcaa cgcagcggaa agcgccaggt ttgcagactc
     4321 tgagcaactt tactgcccag tacgcctgcg cccaaaatgc cgatggtaaa atcttcccga
     4381 tgatattcag gcagcggttg ccaatgcgaa ctattttgct ggatgcgata atcgtcaaaa
     4441 cgtcgaaacc aatgcagcac ctgactgaca gcatattcct gcatttgctc gcccataccg
     4501 gtatcttcca ggcgaaaaag tggaacagaa gggttcagca tttcagggtg tgcctgtagc
     4561 ttgctcaaaa tagaatcaac accggccccg agtgcgaaca ccgctttaag atcgcgccct
     4621 gccagcattt caacaggagg atgccagact aaagcataat cagcagagtc attatctccg
     4681 cttttccatg ctctgactct tgcctgagga atagctttgc gcagtgcctc aatccaccat
     4741 tgggtatcga acgttgggtg ataaaagatg atatccatac tgactcccga aaagcgttgt
     4801 gcgaaattta ttcgcactta tcgttatgat ctacaaaggc caccagcata acaaatccgt
     4861 ggtcggtggc aaaaaaagca gatttcgctt attaaaacca cacattgatt gaagtttgaa
     4921 taaacgcgcg attttttcaa aaagtttgtt gacctcaggt catgatttcc ctaaattagc
     4981 gcccgttcca gcaagacagg aac
//
LOCUS       DAJVXP010000205         4882 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVXP010000205 DAJVXP010000000
VERSION     DAJVXP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.206"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(358..1401)
                     /locus_tag="OQS10_003923"
     CDS             complement(358..1401)
                     /locus_tag="OQS10_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS2294816.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1813..2997)
                     /locus_tag="OQS10_003924"
     CDS             complement(1813..2997)
                     /locus_tag="OQS10_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2294817.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3297..3391)
                     /locus_tag="OQS10_003925"
     tRNA            complement(3297..3391)
                     /locus_tag="OQS10_003925"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Sec,seq:tca)
     gene            3684..>4882
                     /locus_tag="OQS10_003926"
     CDS             3684..>4882
                     /locus_tag="OQS10_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS2294818.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1276 a         1069 c         1117 g         1420 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaaataata taataacaaa
     1741 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaat atcagggaaa
     1801 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
     1861 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
     1921 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
     1981 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
     2041 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
     2101 aaagactagg ggatgatgtt ttgagattgg ttcaagctgc ttaaatagca taatgacctg
     2161 ctcagagagc ggcacaatat gaggtcgctt catcttcatt acttctgccg ggatctccca
     2221 caattttgtc tcaaaatcga tatcttccca gcgtgcaaaa cgcagttctt gtgttcgtac
     2281 gccggttaac ataatgatct gagtagctgt cttagtgatt atacttccgg tataacccgc
     2341 cagatcgttg aggaagtggg gaagttcatt agcagtaaga aagggaaaat gtactttttt
     2401 cggtgtagct aaagcactgg caagatcggg ggctgggtta tagtctgccc ggccagtaac
     2461 aattgcatag cggaacacct caccacagcg ctggcggacc ttgcgcattt tctccagagc
     2521 tccacgtttt tccattttac gcagtgcttc cagtaactcc attggtttga tttcggcaat
     2581 agggcgcttg ccaatgtagg gaaaaatgtc tttttcaaag gtatcaatga tttcatcacg
     2641 atagcgtaat gaccaccgat cagcttttga ctggtgccat tcgcgagcaa tagcctcgaa
     2701 cgtatttttc agactcattt gctgcgcgat tttttcttcc ttttttgcct cccctggatc
     2761 gcctccagca gcgaggagtt tcctggcttc actgcgtttc tcgcgagcat cggctagcgt
     2821 tacatcgggg tatacgccaa tagaaagcat cttttcttta ccagcaaagc ggtacttcat
     2881 gcgccagtat ttagaaccat tagtgttaat cagtagatac ataccgccgc catcagcaag
     2941 cttatagggt ttgtctttgg gttttgcggt tttcactttg atatcggtta atgccatgac
     3001 atcctccggc ttttgatgaa ttcatgttcc tgagggggat ttctgccagt aactaacctg
     3061 atacccccaa tacacccctt gtataacaaa gatattgtta aaagccgttg ttatgtatca
     3121 catttttcct attatggggg tgcttgtatt tcaataggaa ataacacccc ctaaagtacc
     3181 cccaaaagaa acttgatatg gggtgaagta ggttgacctc aggatacaaa taaatactga
     3241 tatacaaggg attagaagag atttattgac attgggagag ttcagaagag gtaaactggc
     3301 ggaagatcac aggagtcgaa cctgcccggg accgctggcg gccccaactg gatttgaagt
     3361 ccagccgcct caccggagac gacgatcttc cgcgcctcga ttgctacatg gaggcggggc
     3421 gcattatagc tacttccttg agtttctaca tcccccagat cagttttttc cgcctaatac
     3481 acgcagcgta ttatcacgaa agcattataa ctcatataaa acccatattt tacaatgata
     3541 tatgagcgga attaacgcta ccacgatcac ataaaacaag aatcataaat taataaccag
     3601 atatcggaat attcgctctc acagggatgg tttacaaaat gcgttatcgg tctcagcacc
     3661 cctatagcat caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg
     3721 gttatggcat gggagacgcc gccagccaca ttattttcga taacgtaatg ttatatatga
     3781 tgttctttta taccgatatt tttggcattc ctgccggatt tgtcggaacc atgtttttgg
     3841 tcgctcgtgc actggatgcg atttccgatc cttgcatggg gttgttggcc gatcgaacgc
     3901 gctctcgctg gggtaaattt cgtccgtggg tactgtttgg cgcactgcca ttcgggatcg
     3961 tctgtgtact ggcctatagc acgccagatc tcagtatgaa cggcaaaatg atctatgcag
     4021 caattactta caccctactt accttacttt ataccgtcgt caatatccct tactgcgcat
     4081 tgggtggtgt aatcaccaat gacccgactc agcgtatctc gctgcaatcc tggcgttttg
     4141 tgctggcgac cgcgggaggc atgctttcta ctgttctgat gatgccactg gttaatttaa
     4201 ttggcggtga taataaacca ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg
     4261 cattcatgat gctggcattt tgtttcttca ccactaaaga acgcgttgaa gcaccaccta
     4321 caacaacgtc tatgcgggaa gatttacgtg atatctggca aaacgaccag tggcggattg
     4381 tcggtttact aaccattttc aatatcctgg cggtgtgcgt acgcggtggg gcgatgatgt
     4441 attacgtcac atggattttg ggcacgccgg aagtgtttgt cgcttttctc accacttatt
     4501 gcgtgggtaa cctgattggt tccgcactgg caaaacctct gaccgactgg aaatgtaaag
     4561 tcactatctt ctggtggacg aacgccctgc tggcagtgat tagcctcgcg atgttctttg
     4621 ttcccatgca ggccagcatc actatgtttg tcttcatctt cgtgattggt gtgttgcatc
     4681 aactggtgac acctatccag tgggtaatga tgtccgatac cgtcgactac ggcgagtggt
     4741 gcaatggtaa acgcctgacc gggatcagtt ttgctggcac gctgtttgtg ctcaaactgg
     4801 ggttggcctt cggcggcgct cttatcggct ggatgctggc ttatggcgga tatgatgcgg
     4861 cagaaaaagc gcagaacagc gc
//
LOCUS       DAJVXP010000206         4873 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVXP010000206 DAJVXP010000000
VERSION     DAJVXP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.207"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..108)
                     /locus_tag="OQS10_003927"
     CDS             complement(<1..108)
                     /locus_tag="OQS10_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2294819.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIIS"
     gene            complement(108..1028)
                     /locus_tag="OQS10_003928"
     CDS             complement(108..1028)
                     /locus_tag="OQS10_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS2294820.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1166..2065
                     /gene="pgrR"
                     /locus_tag="OQS10_003929"
     CDS             1166..2065
                     /gene="pgrR"
                     /locus_tag="OQS10_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS2294821.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2402..4015
                     /gene="mppA"
                     /locus_tag="OQS10_003930"
     CDS             2402..4015
                     /gene="mppA"
                     /locus_tag="OQS10_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS2294822.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4066..>4873)
                     /gene="ynaI"
                     /locus_tag="OQS10_003931"
     CDS             complement(4066..>4873)
                     /gene="ynaI"
                     /locus_tag="OQS10_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS2294823.1"
                     /translation="DVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMS
                     RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDR
                     PFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNR
                     RITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCF
                     TKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT         1314 a         1092 c         1150 g         1317 t
ORIGIN      
        1 gctgataatc gccggatact gttttgactc atcaaaattt ggtggaaact gaatattggc
       61 agcaatatgc caggccatat cactattgcg gatttgaata tgtttcatta cagagtttcc
      121 tcaaagaacg gagctaatac tgaaattgct tcagccacat aggctttgcc gtcataaaga
      181 tccatatggt ttgcgccttc aacaatgtga tagcgtttat cttggcttga tgctcgatcg
      241 tacaggtcgt cactcatcca tttgctccct gcctggctgc ccgccacaat ctgcatcggc
      301 tgagtcaggt acacttccgc catatggtaa gcatcaaagg taataatctg gttaaggctg
      361 cgcaaagtag cgtaacccgg tgctgttgga tactgcgcgc gaggggtgtg gtaatactcc
      421 caggcctgac gcagttcttc gttcggcgca tcggactcct tcattggtgc cagtggcata
      481 acggcatatt ctccgctgct aatatcgctg gttcgggcgt ttgaacccgc ttcaacgtat
      541 ggcagggcat caatagattt cacattgttt tcccaaccat tacggaacat cgaaccaata
      601 ttgaccgcac taacggtacc gatggccttg atgcggcgat cctgaattgc agcattggct
      661 gtatatcctg caccggcaca aattcccatc gcaccaattc gggtattgtc gacataagaa
      721 agcgttgtca ggtaatcaat cacggcactg atgtcttcag tacgaatgta tgggttttct
      781 aactgacgcg gctcgccgcc actttcacct tgataagatg cgtcataagc aatagtgaca
      841 aatccctttt ccgccagttt tttggcatag gttccggccg tttgttcttt aacgccccca
      901 cctgggtgag ataccacaat tgcctgatac tgacgggttt catcaaattt tggagggaaa
      961 tagatcactg cagacaaaga gatggttgga ttattgctgt tagtgaagct gactttatta
     1021 ttcatcattc cgttcctcat ggagttgtcg gttcgtttta acggttggtg atatcactat
     1081 agatattgat cattaagttg attagaccca aaattatgat tagacctatc atttaaatga
     1141 ttaatagatt gcatggagat actgaatgaa gcgtgaagaa atcgctgatc tgatggcgtt
     1201 tgtcgtcgtt gcagaggagc gtagcttcac tcgtgcagca gcccgcctga gcatggcgca
     1261 gtcagcttta agccagatag tgcgtcgtat agaagaacga ttgggattgc ggcttctgac
     1321 gcgaaccacg cgcagcgttg ttccaactga agcgggcgag catcttttgt ctgttcttgg
     1381 ccctatgttg catgacatag attcagccct ggcatccctg agcgagctgc agaaccgccc
     1441 atccgggaca atacgtatta ctactgtaga acatgcagcc aaaacgatat tgttaccagc
     1501 aatgcgcaca ttcctgaaat cgcatcctga aattgatatt cagctcacca ttgattatgg
     1561 tttgaccgat gtcgtttctg aacgttttga tgcaggcgtc cgtctgggtg gggagatgga
     1621 taaagatatg atcgccattc gaatcgggcc agatatacca atggctattg ttggctcacc
     1681 ggattatttt tctcgccgaa gtgttccaac gtcagtgtca caattaatag atcatcaggc
     1741 aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa tacgcggtgg
     1801 acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag acctgatcat
     1861 tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg ttgagcgggc
     1921 tattaaagaa aaaaaactga tacgtgtttt ggataaattc acaccagatt tacccggtta
     1981 tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat ttatagatag
     2041 gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta atttttctct
     2101 taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgt acaatggcga
     2161 cagatgctga ttatgatacc gaaaactggt ttgaacgtgc gaagcctgaa cgtagtgttc
     2221 ggtgttctat gtgctttacc gcattttgtc gtctattatt tacactaaat ctgatttaat
     2281 atattgatac ataaaacatt ttatgctccc tgtgtcactt ttttgatgga agttgtttgc
     2341 atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta gagggaaact
     2401 tatgaagcac tctgtttcag tcacgtgttg tgcgctgttg gtcagcagca tttctctttc
     2461 gtatgctgca gaagttccga gcggcacagt actggcagag aagcaggagc tggtgcgcca
     2521 cattaaagat gagcctgcgt cgctggatcc cgctaaagcc gtgggcctgc cagagattca
     2581 ggtcattcgc gatctgtttg aaggactggt gaatcagaac gagaaagggg agattgtccc
     2641 cggcgttgcg actcagtgga aaagtaatga caaccgtatc tggactttta ccctgcgcga
     2701 taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct acagctggca
     2761 gcgtctggtg gacccgaaaa cgctatcgcc atttgcatgg tttgccgcgc tggcgggaat
     2821 caacaacgca caggcgatta ttgatggtaa agctacgcca gaccagcttg gcgtcaccgc
     2881 agttgatgcc catactttga aaattcagct tgataaaccg ttgccgtggt ttgtgaattt
     2941 aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagcg gtaaagagtg
     3001 gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagatc gcgtagtcaa
     3061 tgaaaaactg gtcgtggtac cgaataccca ttattgggat aacgccaaaa cggtactgca
     3121 aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt acctcgcggg
     3181 ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt tgaaggatat
     3241 tccggggcag gtttatacgc cgccgcagct cgggacctat tattatgcgt ttaacacgca
     3301 aaaagggccg acggcagatc agcgcgttcg tctggcatta agtatgacga tagatcgccg
     3361 cctgatgacc gaaaaagtat tagggacggg cgaaaagcca gcgtggcatt ttacaccaga
     3421 tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa caaatgagtc aggaagaact
     3481 gaatgcgcag gcaaaaactt tgttgagcgc agctggttat ggtccgcaaa aaccgctgaa
     3541 gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg ctgtagcatc
     3601 gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa aatcaggaat ggaaaaccta
     3661 tatcgatagc cgtaacaccg gcaattttga tgttatccgc gcctcatggg tgggggatta
     3721 taatgaaccc tccactttcc tgacattatt aacgtcaacg cattcaggaa atatttcacg
     3781 ctttaacaat ccggcatacg acaaagttct ggcccaggca tcgacggaaa ataccgttaa
     3841 agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag caccgattgc
     3901 accaatttat caatatacca atggacgatt aatcaagccg tggctgaaag gttatcccat
     3961 taataacccg gaagatgtgg cgtacagccg gactatgtat attgtgaagc attgatgtga
     4021 tgggaactgg cgttaccctt gtggataacg ccagtgatgt ctgatttagc gcccctgttc
     4081 cggcggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt ccgcgccgtg
     4141 tgactgcaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa gccattcagc
     4201 ccataccgtg gttttggtaa agcaataaac cataatattc aatgacgagt cggcaaactg
     4261 gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt tcagcatctc
     4321 acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta aaccaatggt
     4381 cgtggtaatg cggcggttgg tcattcgtcc tgggttttct acgctgatcg acgaaaacag
     4441 cgagttcggt acgtacaatg gacgattatc aaaggtcgta attttggtaa ttcgccagcc
     4501 aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt cgccaatact
     4561 gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa ttactcagaa tatctttacc
     4621 ggccataccg acagccagac caccaatacc accaaaggtc agcaagccag aaaggctcat
     4681 gccgaaatgt tcgccataaa gcagaacaag caccacaata atggtgattt tgataatgcg
     4741 cgacataatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt tttcaaactg
     4801 attaatcagc aaaaatagct taatcgtcag aataaccgca atcagggacg tacagataaa
     4861 atcgataacg tct
//
LOCUS       DAJVXP010000207         4796 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVXP010000207 DAJVXP010000000
VERSION     DAJVXP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.208"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            6..81
                     /locus_tag="OQS10_003932"
     tRNA            6..81
                     /locus_tag="OQS10_003932"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OQS10_003933"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OQS10_003933"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OQS10_003934"
     CDS             complement(299..1057)
                     /locus_tag="OQS10_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS2294824.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OQS10_003935"
     CDS             complement(1065..2168)
                     /locus_tag="OQS10_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS2294825.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="OQS10_003936"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="OQS10_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="OQS10_003937"
     CDS             complement(3427..4452)
                     /locus_tag="OQS10_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS2294826.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1239 c         1145 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcccggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAJVXP010000208         4787 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVXP010000208 DAJVXP010000000
VERSION     DAJVXP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.209"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(63..506)
                     /locus_tag="OQS10_003938"
     CDS             complement(63..506)
                     /locus_tag="OQS10_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS2294827.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            663..1592
                     /gene="metA"
                     /locus_tag="OQS10_003939"
     CDS             663..1592
                     /gene="metA"
                     /locus_tag="OQS10_003939"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS2294828.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1861..3462
                     /gene="aceB"
                     /locus_tag="OQS10_003940"
                     /pseudo
     CDS             1861..3462
                     /gene="aceB"
                     /locus_tag="OQS10_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3492..4783
                     /gene="aceA"
                     /locus_tag="OQS10_003941"
                     /pseudo
     CDS             3492..4783
                     /gene="aceA"
                     /locus_tag="OQS10_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1194 a         1259 c         1212 g         1122 t
ORIGIN      
        1 aaaaatgccg ggtgcgacgc tggcgcgtct tctccagcct gggcaggcac ggcaggggca
       61 gcttattcct ccacatacgc cagattcagc aacggatacg gtttccccaa atcgtccacc
      121 tcagagcgtc ccgtaacctt aaaacccacc ttcttataga acccaaccgc ctgctcattt
      181 tgctcattaa cgttggttgt cagttccggt gccatcgaga gcgcatgctc caccagcatc
      241 cgacccacgc cgcagccgca cacatcagga tcgataaaca gcgcatccat atgctgccca
      301 cttaacaaca taaacccaac cggctgatcc cgttcattaa ccgcgaccca caacggcgct
      361 tccggcagga aggaacgaac tagcacctcc agctcggccc gatactctgc tgataaaaaa
      421 tcgtgtgtgg catcgacaga acgacaccaa atcgcaacga gttcctcccc ttcctcatgc
      481 cgtgagcggc gaatactaat aaccattttc tctccttttc gtcattctta tattctaacg
      541 tagccttttc cttgaaactt tctcaccttc aacatgcagg ctcgacattg gcaaattttc
      601 tggttatctt cagctatctg gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg
      661 ttatgccgat tcgtgtgccg gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg
      721 tctttgtgat gacaacttct cgtgcgtctg gtcaggaaat tcgtccactt aaggttctga
      781 tccttaacct gatgccgaag aagattgaaa ctgaaaatca gtttctgcgc ctgctttcaa
      841 actcaccttt gcaggtcgat attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca
      901 cgcccgcaga gcatctgaac aacttctact gtaactttga agatattcag gaacaaaact
      961 ttgacggtct gatagttacc ggcgcgccgc tgggcctggt ggagtttaat gatgtcgctt
     1021 actggccgca gatcaaacag gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt
     1081 ttgtctgctg ggcggtacag gccgcgctca atatcctcta cggcattcct aagcaaactc
     1141 gcaccgacaa actctctggc gtttacgagc atcatattct ccatcctcat gcgcttctga
     1201 cgcgtggctt tgatgattca ttcctggcac cgcattcgcg ctatgctgac tttccggcag
     1261 cgttgattcg tgattacacc gatctggaaa ttctggcaga gacggaagaa ggggatgcat
     1321 atctgtttgc cagtaaagat aagcgcattg cctttgtgac gggccatccc gaatatgatg
     1381 cgcaaacgct ggcgcaggaa tatttccgcg atgtggaagc cggactagac ccggatgtac
     1441 cgtataacta tttcccgcac aatgatccgc aaaacaaacc gcgcgcgagc tggcgtagtc
     1501 acggtaattt gctgtttacc aactggctca actattacgt ctaccagatc acgccatacg
     1561 atctacggca catgaatcca acgctggatt aatcttctgt gatagtcgat cgttaagcga
     1621 ttcagcacct tacctcaggc accttcgggt gcctttttta tttccgaaac gcacctcagc
     1681 aagtgaataa attttattca tattgttatc aataagttat caagtatttt taattaaaat
     1741 ggaaattgtt tttgattttg cattttaaat gagtagtctt agttgtgctg aacgaaaagc
     1801 gcacaacgat ccttcgttca cagtggggaa gttttcggat ccatgacgag gagctgcacg
     1861 atgactgaac aggcaacaac aaccgatgaa ctggctttca caaggccgta tggcgagcag
     1921 gagaagcaaa ttcttactgc cgaagcggta gaatttctga ctgagctggt gacgcatttt
     1981 acgccacaac gcaataaact tctggcagcg cgcattcagc agcagcaaga tattgataac
     2041 ggaacgttgc ctgattttat ttcggaaaca gcttccattc gcgatgctga ttggaaaatt
     2101 cgcgggattc ctgcggactt agaagaccgc cgcgtagaga taactggccc ggtagagcgc
     2161 aagatggtga tcaacgcgct caacgccaat gtgaaagtct ttatggccga tttcgaagat
     2221 tcactggccc cggactggaa caaagtgatc gacgggcaaa ttaacctgcg tgatgcagtt
     2281 aacggcacca tcagctatac caatgaagca ggcaaaattt atcagctcaa gcccaatcca
     2341 gcggttttga tttgtcgggt acgcggtcta cacttgccgg aaaaacatgt cacctggcgt
     2401 ggtgaggcaa tccccggtag cctgtttgat tttgcgctct atttcttcca caactatcag
     2461 gcactgttgg caaagggcag cggtccctat ttctatctgc cgaaaaccca gtcctggcag
     2521 gaagcggcct ggtggagcga agtcttcagc tatgcagaag atcgctttaa tctgccgcgc
     2581 ggcgccatca aggcgacgtt gctgattgaa acgctgcccg ccgtgttcca gatggacgaa
     2641 atccttcacg cgctgcgcga ccatattgtt ggtctgaact gcggtcgctg ggattacatc
     2701 ttcagctata tcaaaacgtt gaaaaactat cccgatcgtg tcctgccaga cagacaggcc
     2761 gtgacgatgg ataaaccatt cctgaatgct tactcacgcc tgctgattaa aacctgccat
     2821 aaacgcggtg cttttgcgat gggcggcatg gcagcgttta ttccgagcaa agatgaagag
     2881 cgtaataacc aggtgctcaa caaagtaaaa gcggataaag cgctggaagt caataacggt
     2941 cacgatggca catggatcgc tcacccaggc cttgcggaca cggcaatggc ggtattcaac
     3001 gacattctcg gctcccgtaa aaatcagctt gaagtattgc gcgaacaaga cgcgccgatt
     3061 actgccgatc agctgctggc accttgtgac ggtgaacgca ccgaagaagg tatgcgcgcc
     3121 aacattcgcg tggctgtgca gtacatcgaa gcgtggatct ccggcaacgg ctgcgtgccg
     3181 atttatggcc tgatggaaga tgcggcgacg gctgaaattt cccgtacctc gatctggcag
     3241 tggatccatc atcaaaaaac gttgagcaat ggcaaaccgg tgaccaaagc cttgttccgc
     3301 tagatgctgg gcgaagagat gaaagtcatt gccagcgaac tgggcgaaga acgtttctcc
     3361 cagggccgtt ttgacgatgc cgcacgcttg atggaacaga tcaccacttc cgatgagtta
     3421 attgatttcc tgaccctgcc aggctaccgc ctgttagcgt aaaccaccac ataactatgg
     3481 agcatctgca catgaaaacc cgtacacaac aaattgaaga attacagaaa gagtggactc
     3541 aaccgcgttg ggaaggcatt actcgcccat acagtgcgga agatgtggtg aaattacgcg
     3601 gttcagtcaa tcctgaatgc acgctggcgc aactgggcgc agcgaaaatg tggcgtctgc
     3661 tgcacggtga gtcgaaaaaa ggctacatca acagcctcgg tgcactgact ggcggtcagg
     3721 cgctgcaaca ggcgaaagcg ggtattgaag cagtctatct gtcgggatgg caggtagcgg
     3781 cggacgctaa cctggcggcc agcatgtatc cggatcagtc gctctatccg gcaaactcgg
     3841 tgccagctgt ggtggagcgg atcaacaaca ccttccgtcg tgccgatcag atccaatggt
     3901 ccgcgggcat tgagccgggc gatccgcgct atgtcgatta cttcctgccg atcgttgccg
     3961 atgcggaagc cggttttggc ggtgtcctga atgcctttga actgatgaaa gcgatgattg
     4021 aagccggtgc agcggcagtt cacttcgaag atcagctggc gtcagtgaag aaatgcggtc
     4081 acatgggcgg caaagttttg gtgccaactc aagaagctat tcagaaactg gtcgcggcgc
     4141 gtctggcagc tgacgtgacg ggcgttccaa ccctactggt tgctcgtacc gatgccgatg
     4201 cagcggatct gatcacctcc gattgcgacc cgtatgacag cgaatttatt accggcgagc
     4261 gtaccagtga aggcttcttc cgtactcatg cgggcattga gcaagcgatc agccgtggcc
     4321 tggcgtatgc gccatatgct gacctggtct ggtgtgaaac ctccacgccg gatctggaac
     4381 tggcgcgtcg ctttgcacaa gctatccacg cgaaatatcc gggcaaactg ctggcttata
     4441 actgctcgcc gtcgttcaac tggcagaaaa acctcgacga caaaactatt gccagcttcc
     4501 agcagcagct gtcggatatg ggctacaagt tccagttcat caccctggca ggtatccaca
     4561 gcatgtggtt caacatgttt gacctggcaa acgcctatgc ctagggcgag ggtatgaagc
     4621 actacgttga gaaagtgcag cagccggaat ttgccgctat accttcgtat ctcaccagca
     4681 ggaagtgggt acaggttact tcgataaagt gacgactatt attcagggcg gcacgtcttc
     4741 agtcaccgcg ctgaccggct ccactgaaga atcgcagttc taagcaa
//
LOCUS       DAJVXP010000209         4772 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVXP010000209 DAJVXP010000000
VERSION     DAJVXP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.210"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..187
                     /locus_tag="OQS10_003942"
     CDS             <1..187
                     /locus_tag="OQS10_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS2294829.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
     gene            complement(384..2321)
                     /locus_tag="OQS10_003943"
     CDS             complement(384..2321)
                     /locus_tag="OQS10_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS2294830.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2732..3439
                     /locus_tag="OQS10_003944"
     CDS             2732..3439
                     /locus_tag="OQS10_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS2294831.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3545..3620
                     /locus_tag="OQS10_003945"
     tRNA            3545..3620
                     /locus_tag="OQS10_003945"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3578..3580,aa:Phe,seq:gaa)
     gene            3818..>4772
                     /locus_tag="OQS10_003946"
     CDS             3818..>4772
                     /locus_tag="OQS10_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS2294832.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1257 a         1247 c         1150 g         1118 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg aatatcagcg tgattcaccg
      361 tcagatttac tgctacgtcg ccctcaggga attaaaccta aaaagaaaat cgtcaaaatt
      421 agagattccg gtttgacacg gaaagtcata tcagcgctat gcgcatcctg ggggttcatc
      481 accactgacg ggaaactctg ctggcggaac atcgcttttt gtaggtcttt gacgtcgaaa
      541 ctgacataca gatcgtgcat ctcctgacac aactggcgca gggtgtagtc gcgatagcgc
      601 accggatatt tgttataaac gctcggcaac acctcagcca gcggcgagtc atcctcaata
      661 tgctgttcaa attgcgccag catcgccacc agttgtgcca gcttctcgtg gctttccgcc
      721 ggagttaata agaacagaat ggagttgaga tcgcacttct ccggcacaat gccgttctca
      781 cgcagatagt gcgccagaat cgtcgccgga acgccaaagt cgctatattc gccggtttcg
      841 gcattgatac ctggtgtagt gagtaacagc ttgcacggat caacaaaata ctgatccgcg
      901 gcatatcctt caaagccgtg ccacttcgcc cccggctcaa aactgaaaaa acggcggtcg
      961 ctggctaaca cagatgtcgg ataatcctgc cacaatttgc catcaacaac gggcgggata
     1021 aacgggcgga acagcttaca gcgcgcaaga atagccttgc gcgactcaat acccaactca
     1081 acgcactcag cccacagccg acgcccactc tccccttcat gaattttggc gttaacatcc
     1141 agtgcagcaa acagcggata gaaagggctg gtagaagcat ggagcataaa ggcgttattc
     1201 aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat ggttatcttt tttatggatc
     1261 tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg actgagtcac aaagatcccc
     1321 ggatcgtttt cgttaagttc taatagcagc ggcgagctat ccgccatcat cgggataaac
     1381 tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat cacacagatg cccaacggta
     1441 tcgatcacct gacgggcgtt atagacagtt ccgtcatagg ttcccagctg aataatcgcc
     1501 aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa cgtcgcgaat ttgctggcgc
     1561 agatactctt cattaaaaca gtgcgcatca ataccgccaa tgaaaccaaa cgggttgcgt
     1621 gaagcttcca gatagaccga cgtcgccccc gcctgaatca gcgcgccgtg atgattcgac
     1681 ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg ttaacagcgc attcgtcacc
     1741 actttattcg ctgccgatgt gccgttcagc acaaaatagg ttttatcggc atgaaagact
     1801 ttggctgcga atttctgcgc atccttcgcc gatccttcat gaataagcag atcgcccaat
     1861 tttacgtcag cgttacacat atcggcgcga aagacgttct caccaaagaa atcgtaaaaa
     1921 tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt gatgtccagg gcaagcaaag
     1981 gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat aaaacggtgg cagcaaattc
     2041 tcttcatact gacaggctgc ggattccagc tccagccact gctgctcgtt gccgttgatt
     2101 accgccgtaa cgcccgcagg taattcaaca gcatgttcgg aatacaaaaa caccggtaga
     2161 tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc gactatccgc agcggtaatg
     2221 acgactgccg cgacatccgt aaaatcagta tctcccaacg ccaccacgcg gcgatgagta
     2281 gaaagtcggg ataccagttc actactggcg gcaatattca ttgatttcat aagcgcaaaa
     2341 cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa ccctgcccca tgagatatgg
     2401 gtcaaactgg tcaccagctc cgacgaccgc cagacatcag taaaagcaga aacgctctga
     2461 ttttactgtt gtcctgcagt gaacgtgcgt taacttcacc gcatgagcag taacatagaa
     2521 agggaaacgt ttcgcgcgaa acggcgataa gcgagagaat gtaaggagat ggcgtgcatc
     2581 gggcaaactc cgtacagaga ggagaaaatt cgcgcaatca tggcaccttt cgctaaggcg
     2641 tgcaagccag gactttgcga ccagacaagc catcaacaag gcaaaaagtt cataataaga
     2701 aaatcaaaca atacagctaa caggaactct tgtggtcatc ggccctttta tcaacgctag
     2761 tgcagtctta ctgggtggcg ttctcggcgc actgctcagc caacgcttac cggaacgtat
     2821 ccgcgtctcc atgacatcta tttttggtct ggcatcgctg gggattggta ttttactggt
     2881 tgtgaaatgc gccaaccttc cagcgatggt tttagctacg ctacttggcg ctctaattgg
     2941 cgaaatttgt ttgctggaga aaggtgtcaa tacagcagtc gccaaagcac aaaatctgtt
     3001 tcgtcactca cgtaagaagc cagcgcatga atcttttatt cagaattatg tcgcgattat
     3061 tgtcctgttt tgcgccagcg gcaccgggat cttcggggcg atgaacgaag ggatgaccgg
     3121 cgatccgagt attttaatcg ccaagtcatt tcttgatttc tttacggcga tgatcttcgc
     3181 ctgctcgctg ggtattgcgg tgtcggtaat tagtatccca ttactgatca tccagctaac
     3241 gctagcgtgg gctgccgcgc tgatattacc gctgaccaca ccgtcgatga tggcagactt
     3301 cagcgccgta ggcggtttat tgctgctggc aaccggatta cgcatctgtg gcattaaaat
     3361 gttcccggtg gtcaacatgc ttccagcact ccttctggca atgccgcttt ccgccgcctg
     3421 gaccgcctgg tttgcctgac aatgcgtgca atagcggcaa agtgatgata gattgtgcag
     3481 tctgcagtaa attgaagaaa tttgattgac gagacgaggc gaatcaggtt taatgcgccc
     3541 cgttgcccgg atagctcagt cggtagagca ggggattgaa aatccccgtg tccttggttc
     3601 gattccgagt ccgggcacca aattcatatc aacggacctc cacggaggtc cgtttttcgt
     3661 ttcagaacgc cacgatttaa gcgttctgcc tacaaatcaa ttctaccgaa ctcaaccaga
     3721 ttccccccac atcaacccca ttgtgtgggt ataattgcgg gcatacccca gttcgacaga
     3781 atttgtaccc tctttaacgc attcagaagg ctgaactatg gcactgactg acgcaaaaat
     3841 ccgggctgca aagcccactg acaaggctta taaactcact gacggggctg gcatgttcct
     3901 gctggtacat cctaatggct cccgttactg gcgtctccgt tatcgtattc tgggtaagga
     3961 gaagactctg gcacttggtg tgtatccaga agtttctctc tccgaagctc gtacaaaacg
     4021 ggatgaggcc cgaaaactga tttcggaggg ggttgaccct tgcgaacaga aaagagctaa
     4081 aaaagtagtc cctgatttac agctctcttt tgaacatatt gcgcgacgct ggcatgccag
     4141 taataaacaa tgggcacaat cacacagcaa taaagtactc aaaagccttg agactcacgt
     4201 tttccccttt atcggcaacc gggatatcac aacactcaat accccggatc tgcttatccc
     4261 tgttcgtgct gcagaagcta aacaaattta tgaaatcgcc agtcgtctgc agcaaagaat
     4321 atctgccgta atgcgttatg ccgtacagtc tggcatcatc agatataatc ctgctctgga
     4381 tatggctggc gcattgacta cggtaaaacg ccagcatcgc cccgctcttg atctttctcg
     4441 cctgcctgaa cttttgtcgc gtattggcag ttacaaaggg caacctgtca cccagcttgc
     4501 cgttatgctg aatttactgg tttttattcg ttccagtgaa ctcagatacg cccgctggtc
     4561 tgaaattgat attgacaatg ccatgtggac tattccagcc gaacgcgaac ccctgccagg
     4621 cgtaaaattc tcacaccggg gctccaagat gcgaacacca catcttgtgc cactcagcaa
     4681 acaggctgta gccatactga cagaactaca gacatgggct ggtgaaaatg gtctgatctt
     4741 tacgggagca catgacccgc gtaaaccaat ca
//
LOCUS       DAJVXP010000210         4608 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVXP010000210 DAJVXP010000000
VERSION     DAJVXP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.211"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..2162
                     /locus_tag="OQS10_003947"
     CDS             <1..2162
                     /locus_tag="OQS10_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS2294833.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="OQS10_003948"
     CDS             complement(2396..3679)
                     /locus_tag="OQS10_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS2294834.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4608)
                     /locus_tag="OQS10_003949"
     CDS             complement(3814..>4608)
                     /locus_tag="OQS10_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS2294835.1"
                     /translation="KQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGK
                     AASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAES
                     SPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALG
                     ISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHEL
                     RSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1179 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgctt
//
LOCUS       DAJVXP010000211         4548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVXP010000211 DAJVXP010000000
VERSION     DAJVXP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.212"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            236..1318
                     /locus_tag="OQS10_003950"
     CDS             236..1318
                     /locus_tag="OQS10_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS2294836.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1507..1890)
                     /gene="quuD"
                     /locus_tag="OQS10_003951"
     CDS             complement(1507..1890)
                     /gene="quuD"
                     /locus_tag="OQS10_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCS2294837.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1976..2116)
                     /locus_tag="OQS10_003952"
     CDS             complement(1976..2116)
                     /locus_tag="OQS10_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCS2294838.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2113..2475)
                     /gene="rusA"
                     /locus_tag="OQS10_003953"
     CDS             complement(2113..2475)
                     /gene="rusA"
                     /locus_tag="OQS10_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCS2294839.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2472..2762)
                     /locus_tag="OQS10_003954"
     CDS             complement(2472..2762)
                     /locus_tag="OQS10_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS2294840.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2755..2925)
                     /locus_tag="OQS10_003955"
     CDS             complement(2755..2925)
                     /locus_tag="OQS10_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294841.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2925..3380)
                     /locus_tag="OQS10_003956"
     CDS             complement(2925..3380)
                     /locus_tag="OQS10_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCS2294842.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3645..4022
                     /locus_tag="OQS10_003957"
     CDS             3645..4022
                     /locus_tag="OQS10_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294843.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1291 a         1018 c          961 g         1278 t
ORIGIN      
        1 atagagcact tataaataac agccgttaat ataaattggc taatagattt atttttattc
       61 agtcaagagc catgaatagg attcgataga aaaaagttca gataaaaata gagatctact
      121 tcacaaatca aacgagaaac caaaacttac atcttgaaat aatcacattg attagatgaa
      181 tatttatcgc gcaatgacat cattttttaa taatagttca aaaaaagggc tcacgatgaa
      241 aaaattaaca gtggcaattt ctgctgtagc tgcatcagta ctgatggcga tgtctgctca
      301 ggcagctgaa atttataata aagacagtaa caagctggat ctgtacggga aagttaatgc
      361 taagcactac ttctcctcta atgatgcaga tgatggtgat actacttatg cccgtcttgg
      421 cttcaaaggt gaaacccaaa tcaacgatca actgactggt ttcggtcagt gggaatatga
      481 attcaaaggc aaccgcgctg aatctcaagg ttcctccaaa gataaaaccc gtcttgcctt
      541 cgctggcctg aaattcggtg actacggctc catcgattac ggccgtaact acggtgtagc
      601 atacgacatc ggtgcgtgga ctgacgtcct gccagaattc ggtggtgaca cttggactca
      661 aacagatgtg ttcatgactg gtcgcaccac tggtgttgca acttatcgta acaacgactt
      721 ctttggtctg gttgatggtc tgaactttgc tgctcagtat cagggtaaaa atgaccgcac
      781 tgacgtaact gaagctaatg gtgatggttt cggtttctcc actacttatg agtatgaagg
      841 attcggtgta ggtgcaacct atgctaaatc tgaccgcact aataatcagg ttatctacgg
      901 taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg gtctgaaata
      961 tgatgcgaac aacatctatc tggctaccac ctattctgaa acccagaaca tgactgtttt
     1021 tggtaataac catattgcca acaaagcaca aaacttcgaa gtagttgcac aatatcagtt
     1081 cgacttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggaaaag acttgggtgc
     1141 gtggggtgat caggacctgg ttgaatatat tgatgtaggt gcaacctatt acttcaacaa
     1201 aaatatgtcc acttttgttg attacaaaat caacctgatt gataagagcg atttcacgaa
     1261 agcatctggc gttgctaccg atgatatcgt tgctgtaggt atggtttacc agttctaatt
     1321 tgattactaa aagatatgtt gcgggaggct ttgcctcccc aacatataag tggctccctc
     1381 aagccacttc ctttaggagc acaaccttgc ttctaactat ataaaccttc tgttatatat
     1441 taccctttat ttttgggggc gttgcaacgc cccattttta ataattttta gtaaacaatt
     1501 ggcatattaa ttagagttat taacaacgat atccatctct aaccggatat ctaatgccat
     1561 taacatccct tcaattatgc cctcagcctt ctgtaacctt ttcccgatat aaccatcaga
     1621 gcagcaatgc ttacctgcca gtgacatgaa tgtcataccg actacataat aatctactaa
     1681 taaatcgtgc aaatcgctgt tgttcttttt cagacgggcc atgcacccgc aaataatcat
     1741 cgcgtcatcg tcacaacatt gcgggcgaga ttttactttt gaagtaatta atcccttaaa
     1801 accggcggca atggacgacc aggtcacatc ttcatgatta ttagccgccc acgctcccca
     1861 acgctcaaga accatctgaa tatcacgcat caacttactc cacaaaaatc agaccagaac
     1921 gccaattaca agcaaaaatc aacaaaacag tattagttga ttgttatctc tgacttcata
     1981 ctcctgctcc tgtcagggtt ttggcgtaat tcttcagtat tcggtaatcg gtcaaaacag
     2041 aaccggggaa acgatataag cgcagatgcc cccagcggtg gcgaagaagt tctgccatat
     2101 aaaactcaaa catcattcat tccccatttc ggtgatggtc agttccagcc tcccaccttt
     2161 ggtaacaggc atcttcacaa cgcggtaatc aacgacctga gcatcatcca gccagaaacc
     2221 tgctttggtg agtgcgtcaa aagcggcttt ttgcagatta tccaggtcac ggcgacggcg
     2281 atccggcatg tggcactcaa tacggatttt cactggcata gccaggccga tatccagcat
     2341 tgcgttttta atgattcggg tgacgttatc gcggtatgcc tgcccttctg cgctgatgtg
     2401 tgtgcgcccg cgattatggc ggtaatagcg gttattgctc ggaggccagg gtaatgtgat
     2461 gctgtaggta ttcacgcctt aataaccccc tctttcagcc agataacctg cgttctcgcc
     2521 ataccttcca gcgcgcattc ttttgcatac tcagcatcga caaaatatgt gcggcggtcg
     2581 atttcgtcgt gacaggcaga acatgcaatg gtggcaatca ggtctggcgg tttgataccg
     2641 gtaccgcaca atccagccag ccggatatgt gccagtactg acgtttcagg attgccatta
     2701 catacgccag ggattcttac ctggcattcc cgaccacgcg ctgcttttct caaatcagcc
     2761 atgattcctc cttgctgcca gtcgcaacca ttttttatca accaggctag cggtatatcc
     2821 gagcagtgtt ggtatttcgg atggcttcag ctcaggctta cgcttacgac gatttgatac
     2881 tctgtagatg tgcccgttca tgacacgaat aagcggtgta gccattacgc ctcctgctta
     2941 tcgcgcagct gctggtattc acagctctgc ggaattgtca ggtggcagcc gatattcatc
     3001 gcccaggctt cgactttgca caggaagatg tacatctcgc cggtttccag ctctgacgta
     3061 tggcggaggg attggacggt ggtgacctcg ccggacacga catctacacg gtctttgctt
     3121 tcgtagccga gataggtgtg cttcatcgcg tctttgaccc actcaggcgt agcgaaggtc
     3181 ttgccgcggg cgatgagata ttcgctgatc tccgtgtacc acatgtggct gagcgcgttc
     3241 tgcgacaggc tgcgcttctc gcgccacggc ttaacctgca ggcgtaagca ttgcccggca
     3301 tccagcaatg gctgaatctg ctggccgatg gccgcgaagt taccgcgatg gagtttgatg
     3361 ccgtctactg gcagagtcat acggcctccc taacggaaac cgcagaatgc agaaaatcgc
     3421 aggtgcattt ctgcatctgt gacaaggtga tgagttcaga ttgtggtcgc atttaagtcc
     3481 ccttaaatgc gcagaagtca ccggagttgt tcaggctccg atgagaggat tatggcggat
     3541 tgattacgat aaatcaaaac tatcttgaag ccaaaacagt gatcttcgct ttaaaaacct
     3601 tgtatacttt cacctaatca cagccagaac tatggatcta accaatgacc aacaaagaaa
     3661 acaaagataa atatattaac aagttcaaga aagagctttc aaaatatata caaccaactg
     3721 ctggagtgga cattcaatta tttaactcta aggatggtgg tggtgtaatt aaagccactt
     3781 tgaatagaag tggaaaaaga aaatcatcta ttgctggtaa ttttactaaa ttaggtgagg
     3841 caattacctc gagtgggcag agggtttttg gtggtgatct atcaaatgtg aatttttacg
     3901 gaacaaatac gatttttgat ggagatacaa tatttttaat taaatcacct gattcagagg
     3961 agtggtcatc acaaaaagct tccaaagacg tagagggcat cgtatttgga gggaaaaaat
     4021 gattaacatt actactaagg atgcactcaa gtcagtggtg aagtaagaga tctagtaatt
     4081 cttcttgaaa ctgatggaaa aacggtgtct gattatttgc agccaatccc tcccaaaaaa
     4141 gatacacact ggagcatgat tttcattccg gtagcaattt atatactact taatcttgta
     4201 tacattattg ctcacccatt atttcctgaa aaatatctaa aaacatcaac atctatattt
     4261 ctattcattt ccttcatatt aagtgcaata gtagcatatt ttgttttcca taagcataaa
     4321 tctgccactc tatccatatg cagcttagtg ttcttagtgt taattgtagg tgctaacgta
     4381 gggtttatca gttacagcga cctaacacaa aaagcgacgc aaaaattaga tagtttgtct
     4441 aaataattat gttgccatgc gaagtatgca tggctgcatg tctgccttcc atttaaaatg
     4501 ggctaagacc tataacccta aatattattc tttattatct tctttacc
//
LOCUS       DAJVXP010000212         4401 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVXP010000212 DAJVXP010000000
VERSION     DAJVXP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.214"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(139..3654)
                     /locus_tag="OQS10_003958"
     CDS             complement(139..3654)
                     /locus_tag="OQS10_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2294844.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4401)
                     /locus_tag="OQS10_003959"
     CDS             complement(4106..>4401)
                     /locus_tag="OQS10_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS2294845.1"
                     /translation="NKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMS
                     HQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1140 a         1126 c          904 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt a
//
LOCUS       DAJVXP010000213         4325 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVXP010000213 DAJVXP010000000
VERSION     DAJVXP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.215"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..579)
                     /locus_tag="OQS10_003960"
     CDS             complement(<1..579)
                     /locus_tag="OQS10_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS2294846.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OQS10_003961"
     CDS             complement(642..1091)
                     /locus_tag="OQS10_003961"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS2294847.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OQS10_003962"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OQS10_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1090 c         1167 g         1041 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactg
//
LOCUS       DAJVXP010000214         4284 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVXP010000214 DAJVXP010000000
VERSION     DAJVXP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.216"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..656
                     /gene="phnP"
                     /locus_tag="OQS10_003963"
     CDS             <1..656
                     /gene="phnP"
                     /locus_tag="OQS10_003963"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS2294848.1"
                     /translation="RRQPCSGVVKFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHM
                     DHVQGLFPLRWGVGDPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVT
                     PLPLNHSKLTFGYLLETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRAD
                     APRNHCDLNTVLALNQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(803..1132)
                     /gene="yjdP"
                     /locus_tag="OQS10_003964"
     CDS             complement(803..1132)
                     /gene="yjdP"
                     /locus_tag="OQS10_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS2294849.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1201..1518)
                     /locus_tag="OQS10_003965"
     CDS             complement(1201..1518)
                     /locus_tag="OQS10_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS2294850.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            1771..3756
                     /gene="yjcS"
                     /locus_tag="OQS10_003966"
     CDS             1771..3756
                     /gene="yjcS"
                     /locus_tag="OQS10_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS2294851.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            3965..4240
                     /locus_tag="OQS10_003967"
     CDS             3965..4240
                     /locus_tag="OQS10_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294852.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         1081 a         1129 c         1033 g         1041 t
ORIGIN      
        1 atcgccgcca accgtgcagc ggcgtagtga agtttaacga ggcgatcacc ctgatcgacg
       61 ccgggctgca cgatctcacc gatcgctggt ctcccggatc gttccagcag tttttgctga
      121 cgcattatca tatggatcac gtccaggggc tgtttccgct gcgctggggc gtgggcgatc
      181 cgatcccgat ctacggcccg ccggatgaac agggctgcga cgatctgttt aaacatcccg
      241 ggctgcttga tttcagccac acggtggaac cgtttgtggt gtttgatttg caggggttgc
      301 aggtcacgcc gctgccgctt aaccactcaa aactcacttt cggctattta ctggaaacgg
      361 cgcacagccg ggtggcgtgg ctgtctgaca ccgctggctt gccggaaaaa acgctgaaat
      421 ttttacgcaa taatcagccg caggtaatgg tgatggattg cagtcacccg ccgcgcgcgg
      481 atgcaccgcg taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct
      541 cgccacgggt gattctgacc catatcagcc accagtttga tgcgtggctg atggaaaacg
      601 cactaccgtc agggtttgag gtggggtttg atgggatgga gattggggtg gcgtgatgag
      661 agggaatgtg cgcgctggcc ccctcaccct aaccctctcc ccagaggggc gaggggaccg
      721 attgtgctca atattgaata ttgcgctcgt tttctccctc tccccattgg ggtgaggggc
      781 gatgcctgct ccatacccaa cctcatcgcc catactcatc ttccattctc cgctcttcat
      841 cctccagttg ccgacgctcc tgatcaagct ggcgctggcg atcgtccagc tgcctgcggc
      901 gatcttcaaa ctggcggcgg cggtcgtcat attgtctgcg ccgatcgtcg ctcacttcac
      961 gctgccagcc gtcgtcgcgc gaatcttcat agtctcgccc acggtcaggg ttataagcgt
     1021 cattaatcgc ctgctgaata ttaccaatag tatcgtcgat aatatcagcc tgggccggaa
     1081 cggtggacag cgtgagcagg gtgaataaaa gaaatagcgg aaagcgtttc attagccaac
     1141 ctcaaaaaga aactctatcc acattagtca ttactcatcc atgcaagtag tggatgaatc
     1201 tcaatttctc cgctgctcta ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc
     1261 ttcgtactgt gcgcccagcc acgcatccac aatcattttt gccagttcga ggccaaccac
     1321 tcgtgaacca aaagccagtg caacttcact ggcataatgc ggataatcag tgcgctctga
     1381 cgacctggtg tctttatcaa tcacctcact cattgaccat gaataaaagg ctggcacagc
     1441 aacgcagaat cctgcggtgc cagcgacatg accgcccatt gctccggtac gttttttaaa
     1501 aagaaacacc ggttgcattg ataaattgtg ctcagtattt gccgaagtac aatatatata
     1561 ataaatatat aaaacacctc atgttattta cagagataat aaattcgcgt aattttccct
     1621 cttcagcaca aatgcctaat aaaatggatc gttcagctca cacttccact tattaaagtc
     1681 gcaaattccc aattatgata accccagctt taaatgacgt attttgcatg gattacatat
     1741 ttacgacata aatgataaat ggagatttac atgaataact ctcggttatt ccgtttgagc
     1801 agaattgtta ttgcgttaac tgccgccagc ggcatgatgg taaataccgc taacgcgaaa
     1861 gaggaagcga aagccgccac tcaatatacc caacaggtta atcagaatta cgccaaatca
     1921 ttaccgttta gcgatcgtca ggattttgac gacgctcagc gtggatttat cgctccgctg
     1981 ctggatgaag gtattctgcg cgatgctaat ggcaaaccat attatcgcgg ggaagattat
     2041 aaatttgata tcaatgcgcc cgcgcccgaa accgttaacc ctagcctgtg gcgtcagtcg
     2101 cagttaaacg gtatttccgg cctgtttaaa gtcaccgaca gaatgtacca ggttcgcggt
     2161 caggatatct ccaacatcac ctttatcgaa ggtgataccg ggattattgt catcgacccg
     2221 ctggtgacgc caccgagcgc aaaagccgcc cttgaccttt acttccaaaa tcgcccgcaa
     2281 aaaccgattg ttgcggttat ttatacccac agccataccg accactatgg cggcgtgaaa
     2341 ggcattatct ctgaagctga tgtgaaatcc ggtaaagtgc aggttatcgc ccctgctggc
     2401 tttatggacg aagccatcag cgaaaacgta ctggcgggta atattatgag ccgccgtgca
     2461 ctttactcct acggcctgct gctggcgcat aaccctcagg gtaacatcgg caatggtctt
     2521 ggcgtaacgc tggcatcggg ctacccgagc atcatcgcac cgaacaaaac catcaccaaa
     2581 accggtgaga agatgattat cgacggcctg gagtttgact tcctgatgac cccaggtagc
     2641 gaagcaccag ccgaaatgca cttctatatt ccggccctga aagcgctgtg taccgccgag
     2701 aacgccacgc ataccctgca caacttctac actctgcgcg gcgcgaaaac ccgcgacacc
     2761 agcaagtgga ccgagtatct gaacgaaacg ctggatatgt ggggtaacga cgcggaagtc
     2821 ctgtttatgc cgcacacctg gccggtctgg ggcaataagc atatcaatga ttatattggt
     2881 aaatatcgcg atactatcaa gtacattcac gaccagaccc tgcacctggc gaaccagggc
     2941 tacaccatga atgaaatcgg cgacatgatt aaactgccgc ctgcacttgc caataactgg
     3001 gccagccgtg gctattacgg ttctgtcagc cacaacgccc gcgcggtgta taacttctat
     3061 cttggctatt acgacggtaa cccggctaac ctgcatccgt atggtcaggt ggagatgggt
     3121 aaacgttacg tgcaggcgct gggcggttct gcccgtgtca tcaacctggc gcaagaagcg
     3181 aacaagcaag gtgattaccg ctggtcggca gaactgctga aacaggtgat tgccgccaac
     3241 ccgggtgacc aggtcgcgaa gaatctgcaa gcgaataact ttgaacagct gggctatcag
     3301 gccgagtccg ccacctggcg cggtttctac ctgaccggcg cgaaagagct gcgcgaaggg
     3361 gtgcataagt tcagccacgg caccatcggt tccccggaca ccattcgcgg gatgtcggtc
     3421 gaaatgctgt tcgactttat gtccgttcgc ctcgatagcg cgaaagccgc gggtaaaaat
     3481 atcagcctga acttcaatat gagcaatggc gataacctca acctgacgct gaacgatagc
     3541 gtgcttaact accgtaaaac actgcaatcc caagctgacg cctctttcta catcagccgt
     3601 gaagatctgc acgccgtgct gaccgggcaa gccaaaatgg cggatctggt aaaagcgaag
     3661 aaagccaaaa ttattggcaa tggcgcgaaa ctggaagaaa ttatcgcctg tctggataat
     3721 ttcgatttgt gggtgaatat cgtaacccca aattaatgtc actcgctttt gggggcgctg
     3781 aagcccccaa aagcatttat tttaaaaata tatacagcca attaataaat aaaatatata
     3841 agttatgaat gcggtgactg aataaagcta tttttattca tcgagaaaat agccatactg
     3901 acgccttcaa tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt
     3961 taatatgcca acagttctct ctcgcatggc gatgcaactc aaaaaaacag cctggataat
     4021 tcccgtcttc atggtttcgg gatgctcatt atctccggca atcccggtga tcggtgctta
     4081 ttatcccagc tggtttttct gcgccattgc cagccttatt ttgatgctca tcacgaggcg
     4141 agttattcag cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt
     4201 tgctctctac gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat
     4261 ggaaagtacg ccgaaaaaag ctcc
//
LOCUS       DAJVXP010000215         4215 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVXP010000215 DAJVXP010000000
VERSION     DAJVXP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.217"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..268)
                     /locus_tag="OQS10_003968"
     CDS             complement(<1..268)
                     /locus_tag="OQS10_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294853.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPM"
     gene            497..1105
                     /locus_tag="OQS10_003969"
     CDS             497..1105
                     /locus_tag="OQS10_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS2294854.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1203..2594
                     /gene="selA"
                     /locus_tag="OQS10_003970"
     CDS             1203..2594
                     /gene="selA"
                     /locus_tag="OQS10_003970"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS2294855.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2591..>4215
                     /gene="selB"
                     /locus_tag="OQS10_003971"
     CDS             2591..>4215
                     /gene="selB"
                     /locus_tag="OQS10_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS2294856.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVK"
BASE COUNT          931 a         1091 c         1263 g          930 t
ORIGIN      
        1 ccatcggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat
      121 tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg
      181 caccgatgcg caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc
      301 tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat
      361 agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa
      421 caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc
      481 gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact
      541 ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa
      601 cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga
      661 agagggcgaa tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt
      721 cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc
      781 gctggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc
      841 ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga
      901 tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac
      961 tatcgccatt gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg
     1021 tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc
     1081 tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac
     1141 aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc
     1201 ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc
     1261 gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt
     1321 tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt
     1381 ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc
     1441 tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac
     1501 aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt
     1561 acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc
     1621 gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga
     1681 tgttggcggc cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga
     1741 ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag
     1801 tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg
     1861 cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag
     1921 atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag
     1981 gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg
     2041 agttgattgc ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc
     2101 cgcaggcagg gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc
     2161 tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc
     2221 tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca
     2281 gcgcagaggt cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg
     2341 gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc
     2401 cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc
     2461 accttgagtc attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt
     2521 atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga
     2581 tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt
     2641 gcaggtgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac
     2701 catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga
     2761 cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca
     2821 cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc
     2881 gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt
     2941 ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg
     3001 ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct
     3061 gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct
     3121 cgcgatcgac cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt
     3181 aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg
     3241 tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat
     3301 cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct
     3361 tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc
     3421 gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt
     3481 ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga
     3541 taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt
     3601 ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc
     3661 gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc
     3721 ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt
     3781 gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc
     3841 ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga
     3901 acctggtcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct
     3961 ggtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg
     4021 gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa
     4081 agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg
     4141 taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac
     4201 cgcgatcgtc aaaga
//
LOCUS       DAJVXP010000216         4192 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVXP010000216 DAJVXP010000000
VERSION     DAJVXP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.218"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQS10_003972"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OQS10_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS2294857.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OQS10_003973"
     tRNA            complement(330..405)
                     /locus_tag="OQS10_003973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OQS10_003974"
     tRNA            complement(412..486)
                     /locus_tag="OQS10_003974"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OQS10_003975"
     tRNA            complement(603..687)
                     /locus_tag="OQS10_003975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OQS10_003976"
     tRNA            complement(696..771)
                     /locus_tag="OQS10_003976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OQS10_003977"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OQS10_003977"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS2294858.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OQS10_003978"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OQS10_003978"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS2294859.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OQS10_003979"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OQS10_003979"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS2294860.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJVXP010000217         4186 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVXP010000217 DAJVXP010000000
VERSION     DAJVXP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.219"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..324
                     /locus_tag="OQS10_003980"
     CDS             <1..324
                     /locus_tag="OQS10_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2294861.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="OQS10_003981"
     CDS             551..1816
                     /locus_tag="OQS10_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2294862.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="OQS10_003982"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="OQS10_003982"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS2294863.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="OQS10_003983"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="OQS10_003983"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS2294864.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4186
                     /locus_tag="OQS10_003984"
     CDS             3627..>4186
                     /locus_tag="OQS10_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294865.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1092 a          938 c         1005 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcg
//
LOCUS       DAJVXP010000218         4176 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVXP010000218 DAJVXP010000000
VERSION     DAJVXP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.220"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            346..971
                     /gene="gstB"
                     /locus_tag="OQS10_003985"
                     /pseudo
     CDS             346..971
                     /gene="gstB"
                     /locus_tag="OQS10_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(968..2083)
                     /gene="yliI"
                     /locus_tag="OQS10_003986"
     CDS             complement(968..2083)
                     /gene="yliI"
                     /locus_tag="OQS10_003986"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS2294866.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2194..2577)
                     /gene="bssR"
                     /locus_tag="OQS10_003987"
     CDS             complement(2194..2577)
                     /gene="bssR"
                     /locus_tag="OQS10_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS2294867.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2790..4115
                     /gene="rimO"
                     /locus_tag="OQS10_003988"
     CDS             2790..4115
                     /gene="rimO"
                     /locus_tag="OQS10_003988"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS2294868.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
BASE COUNT         1060 a         1054 c         1044 g         1018 t
ORIGIN      
        1 ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa atgcagaacc
       61 cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc gagtatccat
      121 tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac gcacacacaa
      181 acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc tgccgcttcg
      241 ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta cttcaccatc
      301 ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat tacgctgtgg
      361 ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa ctggaactac
      421 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
      481 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
      541 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgcctgtgga
      601 tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc
      661 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
      721 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
      781 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
      841 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
      901 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
      961 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
     1021 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
     1081 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
     1141 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
     1201 aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
     1261 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
     1321 ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
     1381 ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt ccttgcggat
     1441 tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata aaaggattat
     1501 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
     1561 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
     1621 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga
     1681 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
     1741 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
     1801 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
     1861 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
     1921 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
     1981 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
     2041 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
     2101 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
     2161 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
     2221 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
     2281 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
     2341 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
     2401 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
     2461 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc
     2521 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
     2581 tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt ttgagcaaaa
     2641 tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc ttcgcctccg
     2701 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
     2761 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
     2821 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
     2881 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
     2941 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
     3001 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
     3061 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
     3121 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
     3181 aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta
     3241 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
     3301 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
     3361 tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact ggcttccaca
     3421 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
     3481 tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga
     3541 tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
     3601 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
     3661 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctagcg
     3721 agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt ctggatcgcg
     3781 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
     3841 ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
     3901 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
     3961 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
     4021 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
     4081 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
     4141 tgtccgccct gcgaataatg attactgcca gctaat
//
LOCUS       DAJVXP010000219         4149 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVXP010000219 DAJVXP010000000
VERSION     DAJVXP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.221"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..551)
                     /locus_tag="OQS10_003989"
     CDS             complement(<1..551)
                     /locus_tag="OQS10_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS2294869.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRA"
     gene            complement(544..954)
                     /locus_tag="OQS10_003990"
     CDS             complement(544..954)
                     /locus_tag="OQS10_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS2294870.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(960..1493)
                     /locus_tag="OQS10_003991"
     CDS             complement(960..1493)
                     /locus_tag="OQS10_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS2294871.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1471..2559)
                     /locus_tag="OQS10_003992"
     CDS             complement(1471..2559)
                     /locus_tag="OQS10_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS2294872.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2556..3947)
                     /locus_tag="OQS10_003993"
     CDS             complement(2556..3947)
                     /locus_tag="OQS10_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCS2294873.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
BASE COUNT          980 a         1230 c          992 g          947 t
ORIGIN      
        1 gcacgccata tttccagatt ttcaatatct tcaccgtctg taatggtgtc agcgtaccct
       61 gtggacggca gaccactgac gggtgttccc agttgcatgg caataccatc atccgtattc
      121 cccgccgatc ccggttcgtc tgccacaaca ggcacccgga gaagaccatc ggctgcggtc
      181 accgtcgccg tcgtggtaaa ggtcacctga ttatcacgct ggatctgcgt tcccgctggc
      241 aataccggcg tcccctctac gccatcccag cgcacaaatc cccgggctgc caccgcttct
      301 tttcgcgggc agcgtttgat tctggcgtgc cggtacagcc agtcttcatc acacacgtcc
      361 ggtaacagat ttctggcaag ataatcgata tacccgtaca acgtgtgtac ggctgcagcc
      421 tgtacacggg catacacttc cgcatccata cgacgaagca gcgtatcctg ctcaaagcgg
      481 gttaataaat cgctccggat catagaaata agttgcggga ggccggggcg ataaaactga
      541 ctgtcagcca ttcagttcac tccagatatc atcaaaaata atgttgtgaa tattgccgtc
      601 acgctggtaa atggtaatgg caagtgccag cgagtctgtc ccggtccgga cagcgttaat
      661 atcaagacgg gaagcaacac catcctccac catccacgcc agcgcctcac ggacataatc
      721 tctggcaagc tgcggggttt tatttgtcag cttgctgcgt cgcaacagat acaaacgcga
      781 ccccgtgcga tcattctgaa ccgcaggcca ggtatccccc caccatccaa atatccgtgg
      841 tgcatcatca cgcccggcac gccgccatgt aaaaagcgaa ataatcacag cacgcgtcag
      901 aaggtcgatc gaagccccgg tcgacacggg tcgcccgtta acattaatca tcatgatttt
      961 cagcccatcg gttgatccgg cgtgtcagtg atcccgccac catcaccatt ttcggtatgt
     1021 ttgtgggcat tgtaggcctg ccgcatttgc tgcatgctga gtccgccact gtcgcaattg
     1081 tcagtaatat cggcggtgga ttcgactggc atttcaaagc gcgctttagg cgcgttttta
     1141 aaaattatgg ccttcccggc cccgtccacc acaataccgg aacgggtcag gacaacggac
     1201 tgtcccagat cgtcataaag cgccacttcc ccacgtttca gtcctttcag ccggtaacgt
     1261 ctgtcagcca cgaccacaac cacaccatga gaacgatcgc ccgccggaaa taaagcaacg
     1321 ccctcagcac cgttctgtgc ggcagatgta aaaccataag gttcaaggtg ctcaacgtgt
     1381 tgtttctgat ctccggctat cattttcaac cctacagact gacactttct ggcggaatcc
     1441 accgccgtga tgacagcccg ggaaatcaga ttacgaagag aaaacccgtt catcagaaat
     1501 cctcctcaac atttttttct tcctggcggt aacaggctcg gggagataag catctgccgg
     1561 gccaacccgt aattcagtcg tcgtgccccg gtcgtcctgg ttataggtaa cttctgcgat
     1621 caccagttca tcattatcaa aattattcag cgggtcgaaa acgatgacag ataaccccgg
     1681 acgccataac gcgccactgc cctgccgcca gccctgaacc gtatatgttg tctcacgggt
     1741 aagcgcagcc cgttggcgcg cttcaaattc acaacgggcc ttacaggttg ctgtcgttgc
     1801 tgtgcctgac tgctgaatca acaaagggcg ataacgggtc acgccactgt cctgaatggt
     1861 ctgacgtatt gcggcaatgg tggcctcacc aaaatcatcg tcgttgcccg ggcgctgccc
     1921 actgacctga tattcagaaa accgctctct gatgcttctt tccgtgtcac aggaaagaat
     1981 attctctccc agcaccagca ccagcaccag cgccgtcgcc gctttacctg ttccgggttt
     2041 tcccagaacc agtcgcccgc attcatcgtc ataagccagc gtctgaacct gccccagtaa
     2101 ccggttaaga caatcggcaa cggtttcgcc gtgttccggc tgggcatcaa tcaccgctgt
     2161 ctgcggcacg ccagcatcaa caacggtgat gccaaatggc gcagccagtt cactgactat
     2221 tctgagcagg ttttttccgc tctgctggag tggcaaagca gagcagtcaa ccagatcggc
     2281 tgttttgctt cgcccgacaa ttcccatgct gacgctgctg gcgtcataac gaagcggtag
     2341 tgcctccaca tatccggtga gcacaggctc atccccgata agcacttcaa ccagctcacc
     2401 attttttatc cggggttgat aatcccggct tccgggccag cgggtggtaa tggcaacatt
     2461 aaaatcccgg gcaatacggt taatgcccgc actgatacgg acggatgtcc agccgcccca
     2521 ttcgcgaccg gaaacccgaa gtaaaacggt atcattcatc tgacgggtac ccttaatgcc
     2581 ctgaccggaa caaagcccgg atgagagatg gcatttcgat ccaggatatc agtttcacgg
     2641 gacgcatcgt cgtaccatga cgcagccaga acaagcgcag gcagaacttc cgctggcgtt
     2701 cgctctgcgg tttcctccgt ctgaaccaga cgcgcctgaa tatcccggtt cagttctgta
     2761 cgtaatgacg tcagctgaaa aaacagcctg tcatccgccg tacgcctgag ctcctgttca
     2821 atcgccgcgt tcagcgattt gcgtatgatg gtgagatttt cccgcgtggg tggttgcgct
     2881 ctctcatcct gtcctgtgct ggctgccacg ctgtcaagtg ccggatgaga aacgtgaata
     2941 atatcggact gacgttcagt ggaaccacca acagccacaa ccgcctgctg atttttcacc
     3001 aggcttccgg gttgtggcag tgaagtgacc gcccttgtgg cttcgctgat tgccgtcgtc
     3061 cggatgacag cagccacaag gtttgtctgc tgtttttgcc tgataaccga tgcggaatct
     3121 gtgggccaca ccgcacgcgg tgccagtcct gggtccagcg taatgccgga cattgtcgtt
     3181 atggactgca ccagatcctg tgtgttatcc accagccggg ttccggcccg ccaggtatcc
     3241 tgcaacgtat gcacaaaatc actggcaacc gacggcggca tcagaatgac ggataaatcg
     3301 ccctgcagta gtctcgctcc ggcagaaaca gccgaattaa ccattttgaa agctgtctga
     3361 acggttccca gcatatcggt cgtccgggca ataacatcgt tctgaataaa gtccggtatt
     3421 cccgcgagat caaaatcgcc gaacatgtct tcaatcaact catccaggaa tacggacgac
     3481 tcctccagtt ttctggcggt tgcagctccg gcgaccggaa atgacagttc cccgctctca
     3541 acaaactgaa atgaaacccg gcacatacgg ccttcagtgc cggagtgaga aacggtcacc
     3601 tgcccgtcaa tacagccctg catttcaccg aactgcggat ggatcagcgt ccccggcccg
     3661 gcggtttcaa tcgctgtaat cagcctgtcg cgctgctctg cgtaatcatc accaacgaga
     3721 tacgcattaa tcgtcagccg tcgcgtggca cgcccgagat cttccgtgta cggtttgtca
     3781 cggtttgggt attcatggac ctgaacgcgg cgtccgaacg aaccctcgtc gctctccacc
     3841 gaaaacggaa cgccacgaaa tgaagcgtcg taaagattat cgcgccaatt gatacctgaa
     3901 gatgcagaaa agagtgaaga aagagaaggt aaagaaggta agtccatact gtctccacta
     3961 taaaaaaacc gccaatttct ggcggcttat atattaatca tcattaatat ctttcatatt
     4021 caatttgcaa ttttcgtagg tcactcccga ctcgccatta acattaactc cagctctgat
     4081 attcccgttt tcttttacat ctacaaaaaa ctggcctctt ggcatcttga acgcaaatgt
     4141 aaaaccaac
//
LOCUS       DAJVXP010000220         4076 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVXP010000220 DAJVXP010000000
VERSION     DAJVXP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.222"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..784
                     /locus_tag="OQS10_003994"
     CDS             <1..784
                     /locus_tag="OQS10_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2294874.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="OQS10_003995"
                     /pseudo
     CDS             797..1757
                     /locus_tag="OQS10_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OQS10_003996"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OQS10_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS2294875.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OQS10_003997"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OQS10_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS2294876.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAJVXP010000221         4059 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVXP010000221 DAJVXP010000000
VERSION     DAJVXP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.223"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            334..1223
                     /locus_tag="OQS10_003998"
                     /pseudo
     CDS             334..1223
                     /locus_tag="OQS10_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1223..1513
                     /locus_tag="OQS10_003999"
     CDS             1223..1513
                     /locus_tag="OQS10_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294877.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1834..2124
                     /locus_tag="OQS10_004000"
     CDS             1834..2124
                     /locus_tag="OQS10_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294878.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2124..2543
                     /locus_tag="OQS10_004001"
     CDS             2124..2543
                     /locus_tag="OQS10_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294879.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            2711..3034
                     /locus_tag="OQS10_004002"
     CDS             2711..3034
                     /locus_tag="OQS10_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294880.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3464..>4059
                     /locus_tag="OQS10_004003"
     CDS             3464..>4059
                     /locus_tag="OQS10_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294881.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1349 a          717 c          838 g         1155 t
ORIGIN      
        1 aggtgattca gatgttaaga agtatccctg tgaacaataa atgaccaatc agtaatcatt
       61 ctgtgaaata attgttgcaa tttataatcg catctgttat agtttctata tgggctgata
      121 aaaaagttca tgtcttgaag tggtaacact tggggaggtg tcagatggtt ggcgtatatt
      181 aacctctcat tcagggtata cctcagcgtt ttaatcatcg tactgtttca ggcatggact
      241 tctttatcag tccatcaatc ccccagatat tttaatcaca caggctaatc ggttttatcc
      301 ggttggcctt ttcacgtatg aggtaattta tgaatgtcta aatataaccc aaatcagttt
      361 tataaatttg atgtgcgaat ctctcaactt tcagaagaaa ttaacgcagc agcaagattg
      421 atttatatgg tcatggtcca tagctgtgac ttctatgaag agaaatacaa atcatattca
      481 ccaagtattg accatctcgc ttatatggct ggtgtatcaa agagaacagc aatcagagcc
      541 attgagacac ttgagaaaca cggtttaatt caggtgcaaa agaatcaggg gcgtcaaagc
      601 acttatcgtg tattcacact ggatgataag ccggaattac ttgtaagacc tacatcacag
      661 gaagttgcag cctcagtaag acaaaatgac accactacca gtgacaaaat gtcatggggg
      721 tagtgacaaa atgtcacccg tgttagagtt tagataagtt attagataaa acattagata
      781 ataatattac agataagaat aaagagactg gacttaatga tgatattaaa gaagatgact
      841 ttctttcttt ggatgaatta aatgattttc tctctatgaa tgaatctgac cttgaagtaa
      901 gctctaaaaa gacttctaaa caagacagag aaatcactga tgtgagtgtt gatagtgacc
      961 ataaaaacac acagtgcccc gccactgatg atgagttgga ccccttcgat gatgaagagg
     1021 atgaacgtga aaaagccatt aagagggcag agaagaaaaa gagattcgag cttgcaagac
     1081 aaaagaacaa agtatcgggt ttagacccat tcgatgacag tgaagatgat tattatcttt
     1141 caccgaaaaa gaaacaaact caggatgaca agaagcaaaa aattaaaccg aagatgtatt
     1201 ttgccggtgg aggaattaag taatgaaaga atatcacggt gaaaagagat acaaagatta
     1261 cctgttaaag cgttacagca tttctcgtga aggtcatctt atgaaggata ctcacgggga
     1321 agtttaccgg attagaccaa agaaagaagg gaggggttat ttcttttacg atggtgtgac
     1381 agatttaaag attgacgctt tgaggtttgc agtgctgtat cattttgata tctggaattc
     1441 aatccaccag ctacgattaa aagacggtga ccccagcaac ttaaaggcca ccaatattat
     1501 cacaaaacgt tagtttgttt taccatcatc aatcgtaatt gtgatttcat atttgaaaag
     1561 cttaatgctg aaattgaatc gtgtacttct ggcgataatc atttttaaaa gctgatacac
     1621 aagaagcacc agaaacacaa agcaacctaa gcttgcgaat ctgtgagatt cagcaattgt
     1681 ttcaagaaag cccattaatt ccccttattt atcaaaggga atgacctgta tcagccccct
     1741 acaaccatat taacatgtaa atgagttgtt gacaatatgt tataaatatg ttaaagttag
     1801 ttataagtca aagattttaa gaaggaaaat ttaatgagac acacagttac acgccttttg
     1861 ccagaggcat attccagact ggcagcaatc aaagacaaat acccagccat gagccacaca
     1921 gcacttgttg gtgagatttt gaaggaacac atagccctta caccgtttga cgctatcaag
     1981 ggcgttgcag agggttctaa ggccatcaga atcacaacgg gccaactgga actactcaaa
     2041 gcacagtccg cagtggcagg ttgctcaatg attcaggtgt tgagtgccaa ggttcatcaa
     2101 ctggcggata agctgggggt ttaatgctat gcacatttga ttatcatgcg ttttatcgcc
     2161 atagaggtca ccttgtccgt gagaataacg gaacatggaa agatttaact cgtaatgctg
     2221 aacccttcac acaccagcaa ttaaaatccc gcttctccag atggaagaat gcaccagcag
     2281 atactggtga ttttgagatt atccatacct tcgattatgc ctcagaagat gaccttcacg
     2341 atgcaattaa gctgattgaa gacttcaata cctcagaaga tgtggatggt gacaaatata
     2401 aatcccgtgg taaccgctat aaccgtgatg atatcactca ggccataaat gctggtcaat
     2461 ctcagcgata cattacagaa aaatatggta tttcacggag acagtatttc tacattaagc
     2521 cattgattaa taaagacaat taaaaataac catctaagaa acggtagaat gcactttaca
     2581 ggcagagttg caccttaatt tgataaatta ccttaccagc cagtgcaact ccctctgtaa
     2641 cccgcatggt tactggcctc agaatggcta ttttaagagg tctgaggtgt gtttcatagg
     2701 agatttaaaa atgaataatt acccaatgca aattatggtt gataacgaca ctgctatgat
     2761 ggtgcaaagc ttcattgatg caggtatcgc aattaatttt gataaattac taaagctgat
     2821 ggctgataat gtagaaaaca tctctgactt tattcagtcg gtggagttta acgagcctcg
     2881 aatgatgcta ccaattacag acagcaacat gaaaagattg gttatcgagc aaacgaacat
     2941 atacagtatt tccccagagc aatttttgaa aggtgctgtg acaatcctgt acagtgataa
     3001 tattctggtg acggattctg tgagggttca ctaatgggtt taataattgc ttggacgttg
     3061 cttggcttat ttgttggttg tattattaac aatgcggtaa ataagaattg atgttatcaa
     3121 cctgttaaaa agctgttgac aaataacctt aagcatggca taattttaat tatgggagag
     3181 caattctctc ttatttttct ttcaataatc cgatggatgg atattctgag ttggaaaggt
     3241 gcaatgcttt tcgtcatggc ctttcaggtt agtctgagag gctatctcaa aaagtactct
     3301 tgttcattca ttattgaatc ctttgccctt cacagaaatg tttagggctt tttcagtttt
     3361 taaaagcaca cttggcaggt tcttgttcag tcagactctt cacctctgat tgctgagtgt
     3421 gcttctataa attgaaataa caaataaact aaggggtgaa cgaatgatta gaaatgaaca
     3481 tgaaatgctt atcgaacaca gaccaagtgc acttgatatt cacgaattaa ctttgcgtga
     3541 atcttttgat agagcttggc gggaatcaca cgaagtaaat aaaggtccat atattccacc
     3601 agaaccactc gagattccaa gagttgaaat tgatttttct atgaacgaac ggtgtgaact
     3661 tgatttgaag ttgaagctac aacgccgata tttccaagct attaaagact ctcaagaagc
     3721 aatgagaagt gtttacgaga aaattcagtc caagcttgaa gacgaaattg acgaaatggt
     3781 tatttcgatg gagcttcaaa ccaattctta tgcttatttc tctcgactag accgtgacgg
     3841 ttgggggtat gaccctgtag agattggtaa agtgattgca gatttaccag aaggtcaaag
     3901 agttgttcgt acagaaagag ctgtcagagc accacatcga ctgtctctaa ttgttgctga
     3961 taaatcacaa gctgagcttg aagaaattgc caaagagaaa ctgatgaaag caagagaaaa
     4021 tgagattaat ttgctgaaat caagactggc taagctgat
//
LOCUS       DAJVXP010000222         3972 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVXP010000222 DAJVXP010000000
VERSION     DAJVXP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.224"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            168..503
                     /locus_tag="OQS10_004004"
     CDS             168..503
                     /locus_tag="OQS10_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2294882.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="OQS10_004005"
     CDS             496..978
                     /locus_tag="OQS10_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294883.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="OQS10_004006"
     CDS             complement(1012..1092)
                     /locus_tag="OQS10_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294884.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="OQS10_004007"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="OQS10_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="OQS10_004008"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="OQS10_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="OQS10_004009"
     CDS             2697..3188
                     /locus_tag="OQS10_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS2294885.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..>3972
                     /gene="ygeH"
                     /locus_tag="OQS10_004010"
     CDS             3523..>3972
                     /gene="ygeH"
                     /locus_tag="OQS10_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCS2294886.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKI"
BASE COUNT         1488 a          635 c          638 g         1211 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tc
//
LOCUS       DAJVXP010000223         3959 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVXP010000223 DAJVXP010000000
VERSION     DAJVXP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.225"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..242)
                     /locus_tag="OQS10_004011"
     CDS             complement(<1..242)
                     /locus_tag="OQS10_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2294887.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OQS10_004012"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OQS10_004012"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS2294888.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OQS10_004013"
     CDS             2255..3904
                     /locus_tag="OQS10_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS2294889.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAJVXP010000224         3922 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVXP010000224 DAJVXP010000000
VERSION     DAJVXP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.226"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..301
                     /locus_tag="OQS10_004014"
     CDS             <1..301
                     /locus_tag="OQS10_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294890.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="OQS10_004015"
     CDS             390..2258
                     /locus_tag="OQS10_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCS2294891.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..3922
                     /locus_tag="OQS10_004016"
                     /pseudo
     CDS             2295..3922
                     /locus_tag="OQS10_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1231 a          721 c          769 g         1201 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtatg gtgtcggtga tggcggcgct ggccccggtg aggaacatat agaacgatta
     3721 acacgtatac gcaatattga tggcttacct catgttgatt tcagccgtgt tgataagttc
     3781 ttcacttacg ccgacgcctt tcgcgaaagc ttgcccatta tttcaggtga actttatttc
     3841 gaggctcatc aagggtgctt tacatcagag tcagcaacta aagctcataa tcgaaatatg
     3901 gaaaataaat tacatgatgc tg
//
LOCUS       DAJVXP010000225         3885 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVXP010000225 DAJVXP010000000
VERSION     DAJVXP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.227"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..206
                     /locus_tag="OQS10_004017"
     CDS             <1..206
                     /locus_tag="OQS10_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCS2294892.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
     gene            345..1295
                     /locus_tag="OQS10_004018"
     CDS             345..1295
                     /locus_tag="OQS10_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS2294893.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            1522..2970
                     /gene="uxaB"
                     /locus_tag="OQS10_004019"
     CDS             1522..2970
                     /gene="uxaB"
                     /locus_tag="OQS10_004019"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS2294894.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3142..3450)
                     /locus_tag="OQS10_004020"
     CDS             complement(3142..3450)
                     /locus_tag="OQS10_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2294895.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(3444..>3885)
                     /locus_tag="OQS10_004021"
     CDS             complement(3444..>3885)
                     /locus_tag="OQS10_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS2294896.1"
                     /translation="THNLRGFWAKTAKDNATLERYRRALNIKFNQPEQAARTLSGGNQ
                     QKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIAAQNVAVLLISSDLEEIE
                     LMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQEASC"
BASE COUNT          938 a          920 c          990 g         1037 t
ORIGIN      
        1 cggcattacc ggctaatgac ggtaagcaaa cccccatgcg cgggcatccg gtgtttatcg
       61 ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat aacattccgc
      121 agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc tatcactggc
      181 tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag cattcccagg
      241 gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa tttacttatt
      301 acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat gtacacccca
      361 tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc gctatttttg
      421 cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac gaactttctc
      481 ttttctggcc gctgaatggc gtaatggcgg gggggtttgc ccgctatgtc tggcttaatc
      541 gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc ataaccaccg
      601 aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt gttaccgtcg
      661 ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca gtaagtgcct
      721 tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc ggggcgattg
      781 gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg ttcagtgagc
      841 aattctcaac gggcgtgttg atcgtgcctc tgacggtcat gctgcttgat atcgactatt
      901 tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta agcgtgtttg
      961 cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg ggcggcgaag
     1021 aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg gcggaaaaaa
     1081 tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt tatctcacgg
     1141 taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat gattttaata
     1201 aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg cgcaaccgta
     1261 ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc cgcgcgattt
     1321 gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct cgattaaacc
     1381 atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag ttgatcaaaa
     1441 caggggttcg gcgtattaaa gtttgctaac ctgtcgtacc aattcaaaca ggttgtatga
     1501 ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc cggtgcacag
     1561 tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt tgttgactgg
     1621 caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt tgttcgtccg
     1681 attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtatac caccattatc
     1741 cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg ctcggttaat
     1801 cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca caacccggaa
     1861 atgcgctttg ttttctctaa caccaccgaa gcggggatca gctaccatgc gggcgataaa
     1921 ttcgatgacg cgccagcgat aagctatccg gcaaaactga cccgtctgct gttcgaacgc
     1981 tttagccatt tcaacggtgc gctggataaa ggttggatca tcatcccgtg tgagttgatc
     2041 gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga gtgggcactg
     2101 ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac gctggtggac
     2161 cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga actgggttat
     2221 cacgatggtt tcctcgacac cgctgaacac ttttacctgt ttgtgattca ggggccgaaa
     2281 tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat tgttgacgat
     2341 attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac cgcgctggtg
     2401 ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga tgctgaaatt
     2461 tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga tttgcctcgt
     2521 gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc gtataagcat
     2581 cagctgctgt ctatcgcgct caacggtatg accaagttcc gcacccgcat cctgccgcag
     2641 ctgctggcag ggcagaaggc aaacggcaca cttccggcgc gccttacttt cgcattagcg
     2701 gcattgattg cgttctatcg tggtgagcgt aacggagaaa catatccggt acaggacgat
     2761 gcacactggc tggaacgtta tcagcaactc tggagccagc atcgtgacca tgttatcggt
     2821 acccaggaac tggtagcgat tgtgttggca gaaaaagacc actgggagca ggacctgacg
     2881 caagtgcctg gtctggtcga gcaggttgct aatgacctgg atgcgattct tgaaaaaggg
     2941 atgcgcgaag ctgtacgtcc gttgtgctaa taacaccgtt acttctgaac ccggttttga
     3001 ccgggtttct tatttcacta tattagttac aacatcctat gtgcctttat cattaacaga
     3061 tgccacatgc agcgcctgtt aatgttgtaa gcttgtaggt aatttgtaag tagcgtcttg
     3121 agaatgtgat cgtaatcacg tttaataggg cgactttttc acgcctgaag ataaccatga
     3181 ttgttcgtcc acaacaacac tggctgcgcc gtatttttgt ctggcacggc tcagtattat
     3241 ccaagatatc atcgcgctta ctactttgcg cataagcatt accagcgtcg cgcccattgc
     3301 cagcaggatc aggatttgcg cgctgctata aaccatggtc agcgtttgca cacttaaata
     3361 ctggcggtcg agaaaaccgg gtaatacaaa cagcagcacc accgccagca gtgccgtgat
     3421 ttcacggttg ttctgaataa acttcagcat gacgcctcct gacgctgact atcgccgaag
     3481 gcaacgcgca taatagtctc gacattaata tcgcgcccgg tcagtgcaga gtgggtaatt
     3541 tcgccctgat gcatcacata cacacgatct gccatcagtt cgatctcttc cagatcagag
     3601 gagataagca gcactgccac attctgtgcg gcgatgctgc gcaacagctg gtagatatca
     3661 ttacgggccg agacatccac gccgcgcgtc ggctcatcga caatcaatgc ttgcggcgaa
     3721 gcttccaggc atttggcaat caggattttt tgctggttgc caccggataa tgtccgtgca
     3781 gcttgttccg gttggttaaa tttaatattc agcgcccgac gataacgttc cagggtggca
     3841 ttatctttcg cggttttcgc ccagaatcca cgaaggttat gagta
//
LOCUS       DAJVXP010000226         3858 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVXP010000226 DAJVXP010000000
VERSION     DAJVXP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.230"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="OQS10_004022"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="OQS10_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS2294897.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="OQS10_004023"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="OQS10_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS2294898.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="OQS10_004024"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="OQS10_004024"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS2294899.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="OQS10_004025"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="OQS10_004025"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS2294900.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAJVXP010000227         3798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVXP010000227 DAJVXP010000000
VERSION     DAJVXP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.231"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="OQS10_004026"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="OQS10_004026"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS2294901.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="OQS10_004027"
     CDS             1028..1486
                     /locus_tag="OQS10_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS2294902.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="OQS10_004028"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="OQS10_004028"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS2294903.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="OQS10_004029"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="OQS10_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS2294904.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="OQS10_004030"
     CDS             2692..3369
                     /locus_tag="OQS10_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS2294905.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="OQS10_004031"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="OQS10_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS2294906.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          953 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 tccacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAJVXP010000228         3750 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVXP010000228 DAJVXP010000000
VERSION     DAJVXP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.232"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1361)
                     /locus_tag="OQS10_004032"
     CDS             complement(<1..1361)
                     /locus_tag="OQS10_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS2294907.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="OQS10_004033"
     CDS             complement(1426..1545)
                     /locus_tag="OQS10_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS2294908.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="OQS10_004034"
     CDS             complement(1700..1918)
                     /locus_tag="OQS10_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2294909.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="OQS10_004035"
     CDS             complement(2272..3750)
                     /locus_tag="OQS10_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCS2294910.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1045 a          840 c          660 g         1205 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat
//
LOCUS       DAJVXP010000229         3730 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVXP010000229 DAJVXP010000000
VERSION     DAJVXP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.233"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            65..172
                     /locus_tag="OQS10_004036"
     CDS             65..172
                     /locus_tag="OQS10_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS2294911.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="OQS10_004037"
     CDS             complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="OQS10_004037"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS2294912.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="OQS10_004038"
     CDS             complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="OQS10_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS2294913.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="OQS10_004039"
     CDS             complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="OQS10_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS2294914.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          956 a          937 c          950 g          887 t
ORIGIN      
        1 aatcttgaac ccaaacaact caagaatagc cgcgaaacgt tggcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag aggttaaact
      241 ctgcggcctt tttcgtgctt actgcgggta aggcacccag tcgccgccgt tcaggcgaac
      301 gtacggttta tcctggtatt gaataactac tgcatttgag ttctcggaga ccggtgctgt
      361 ttgtggcaac ccactggtga gttttttcca gtcaacattg tcttcggtga aaatcttgcc
      421 atcaagaacg cgaaccacca gatcggagat agctaggaag ctgctcggtt ggtcaatgac
      481 aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa cggggacgtc
      541 tgtgatagac gggctaggga tatcacgtag gccagatacc tgcattctgt cgcccttcag
      601 cgcgccgccg tgttccggca ccacgaccac catcacttta cgacccgatt tctccagttc
      661 agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt
      721 tttgctgacc cccggataat ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc
      781 actacggctg tttttatctt tttcggtaac gtccagccag cggttaagca cggcggtatc
      841 gtcataaacc ggcgaaccat caaagcccag caaaataacc ggcagatttg tttgatccat
      901 caattcagtc tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc cgaactggcc
      961 gttatgtccc atcatcaggt gctgggtaaa gcccagtttc gaaaggttat caaacagata
     1021 gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg
     1081 cagtaaacgg atcgccgccg ggccactgta ggaggtggcg gagttaaagt tcttgaactc
     1141 aatatcgaaa tgcgaccaca gtggatgcga catcaacccg gcggcttcta tatccgacca
     1201 ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc tgagcatcag cgggcagcga
     1261 agacgggaag gtcgatttac gtttcgcctc cgcgttatag aaattattca gccaggcgtt
     1321 aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata tcacccacta ccgatgcgcc
     1381 acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg
     1441 ttgtccggct ggccacaagg agaaacttgg tcccgccagg gtaagtacgt tcagccatag
     1501 cagtatggca accacaaaaa cggtaatgcg aatccattgt gacaggaata accaggccac
     1561 taataaaaca aaaatggccc caatcatctg ccagttaata aagcgtgtga caaggtcgat
     1621 taaataatcg gtactgaacc ccgccacctg cgaaccctgg ctcattatgc tttccgggcc
     1681 aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc ggcagggcaa tccagtggcg
     1741 caagcgatgc aggctgtagc gcggaatggg catcagcaga aacgtggcaa acaccaaatt
     1801 gaggagcgga tggaagttaa gatatcccgc ccacaacagg ccgaacttaa ccagaaaata
     1861 gaagttccag ccggaaaggc cgcgccagta ttgccagagg gaagaaggca tggcggtatt
     1921 ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg taacgtcccg
     1981 gccggtttaa cataacgagg tttagcaaag aataaacgta aggtgtcgcg aatgcgtcgc
     2041 aaaaagtgcc gcgccagata gcccagcggg aaaaatatca gtgcgcaaac gacaataatt
     2101 tcaatgatat cgctgatggt catcatgatg agcgctccac agcatcatct aacagtcgca
     2161 tgggttctgg tattcgtcgc cagtggcgac catcgtgctc ggcattgatg accggtttag
     2221 aactttgcgt taaaggcagc ggcatgcccc attgttctgg ggcaagcaag cgcatctgca
     2281 ccagctcggc actgatttga tcatcttcaa accagaccat acggtttgag aaaatgtcgc
     2341 cagtaggcaa tgggaaaata tgattcaacg cggtatccag atcgttaatc cgacagaatg
     2401 agagaaacag caccagccga ttaccgccaa tggtcatgat atcgccggta cggttagggc
     2461 gacacagcgt cagggcttgt tcaacgcgga tacccggtac cggacgtagg gcaaccagaa
     2521 cgcctttacc gtgggcaggt aatagagggt tattcatcat gttgttgacg gcattacaga
     2581 acacatccca cttctggaaa ccacgcagtt tgagcggctg ggtcattgac agcaaggtag
     2641 tgatatcttc cggcacatag cgactaaact tctgcccttg cacgctttcg atcatcgtca
     2701 gacaacggga gagtggcgca ttccacggaa taaccatatt tgcaccgcag gccaataaca
     2761 aacgttcatc ggtggcgcgc aggctagcgg tattttcccg cacgaggatt ttcatcgcac
     2821 taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt tgcgcatttt
     2881 gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg ggcttcattg aacaggactt
     2941 cgttattgtt gaacagttgc cagtgttccg atagcggcgg cgcaccttcc agtacagcaa
     3001 cattactcag aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct tcgctttgaa
     3061 ctaatgtcca gataccattt tgttgctgaa cgctaagctg ctgacgggcg ctgacccctt
     3121 tttcgttgca ccagaaggca atatccagca aatgttggtc accctgaaaa cgcaaactgg
     3181 caagaccaaa aagtgaacgg tattcctcaa gcaacaatga aaattgttta tcgttattat
     3241 ttccgggatt aattaccaaa agcgaacaat ggtttaacct gctccattta ttcattttat
     3301 ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca tgcgttattt gcgcaaacaa
     3361 gaataaaaag gtaattatgg ggatcaatag aacactgcaa atcacgggcc aaatagtata
     3421 gacctttttc atgatttagc attgaaaata attttatttt ttccggccct tgagaatcat
     3481 ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg tgtgcggttt
     3541 cagcctggga tgcaattgtt tgattcgcca gactgatagc atcttcatgg cgatcgacgt
     3601 taaaccacca gacgccgcct gctggcatat gtcgcagctc atcccataat gatgagatac
     3661 cgatagagaa tacagggtcc acaatgtccc tcatgtcacc attttgtctg attatcagtt
     3721 tactagcgaa
//
LOCUS       DAJVXP010000230         3675 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVXP010000230 DAJVXP010000000
VERSION     DAJVXP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.234"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OQS10_004040"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OQS10_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS2294915.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OQS10_004041"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OQS10_004041"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS2294916.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..>3675)
                     /gene="dpiB"
                     /locus_tag="OQS10_004042"
     CDS             complement(2048..>3675)
                     /gene="dpiB"
                     /locus_tag="OQS10_004042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCS2294917.1"
                     /translation="FFQRLAFPLRIFLLILVFSIFVIAALAQYFTASFEDYLTLHVRD
                     MAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGDRHSIRLYHLNP
                     EKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVIGVVSIGYLVSK
                     IDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIARVVRQQEALFS
                     SVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPADFFTEQIDEKR
                     QDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQIKQYVESLRTL
                     RHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFADRQVAGLLFGKV
                     QRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLRSDEGNKIVELF
                     LSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLYLIASYVTRCGG
                     VITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          841 a          968 c          893 g          973 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatg
//
LOCUS       DAJVXP010000231         3667 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVXP010000231 DAJVXP010000000
VERSION     DAJVXP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.235"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..253)
                     /locus_tag="OQS10_004043"
     CDS             complement(<1..253)
                     /locus_tag="OQS10_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS2294918.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVG"
     gene            complement(250..1647)
                     /locus_tag="OQS10_004044"
     CDS             complement(250..1647)
                     /locus_tag="OQS10_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS2294919.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            1802..2800
                     /locus_tag="OQS10_004045"
     CDS             1802..2800
                     /locus_tag="OQS10_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS2294920.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(2911..>3667)
                     /gene="ompG"
                     /locus_tag="OQS10_004046"
     CDS             complement(2911..>3667)
                     /gene="ompG"
                     /locus_tag="OQS10_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCS2294921.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT          908 a          974 c          900 g          885 t
ORIGIN      
        1 gcccgacaac acttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
       61 tgcgtttcgg acgtaacgct gcatccatta ccgcttcaac ttgcccctct tcttcaggcg
      121 cttcatcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
      181 ccctgaattt tggattctga tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
      241 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
      301 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
      361 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
      421 gagaggcgca ccatcgctga gccgattacc gcgcaatgcc ggaattttct cgccgttgac
      481 atcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
      541 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
      601 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtt gcggcaaaca gcagtttgtc
      661 gataaccggc gagaataagc ggcgaaagag cgtgcgctgc ccgtagtgga aactttgcat
      721 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
      781 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
      841 cttcaccacc ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
      901 cttatcggcc tgcgccagtt tggactcgcc ccaggtatcg acatccggcc acgggaagaa
      961 ttgcagcgcg ggcgcacctg ccatatctcc tggcaagaca aagcgccctg gctgaataaa
     1021 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc
     1081 cagccggttt tcgtcggcag gtgctagcgg gtccagccct tcgcacatca ttcgccattt
     1141 gaccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgtg accagcttaa
     1201 atagtcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatctc
     1261 cagatacagc gtggaggtgt ctttgaagtg gcgtagcagc gaatcgttcg atttaaaacg
     1321 tagcgccagg cggatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcgcc
     1381 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgagg
     1441 aatgcgtttt acgcccagca ggcgctcttc acgcaccgcg cttaacagcg gcaagcgtgc
     1501 tccggcgtga atattgagca actgattgac catcgcagtg ataaacgctg ttttgccgct
     1561 gcggcttagt ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     1621 attaagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     1681 aatcattcgt aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg
     1741 tcatcgacgg gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc
     1801 tatgtcccct actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc
     1861 acgcgtgctg aataagcaaa ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc
     1921 cattgaagaa ttacagtatc agccaaacaa gctagcccgc gcgctgacct cttcgggttt
     1981 tgatgccatt atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt
     2041 ctctgaagtt ttgcatgcca tcaccgccaa agctgaagaa gaaggtttcg acgtgatatt
     2101 gcagacgtcg cacaacccgg cagaagactt acaaaaatgt gaaagcaaaa ttaagcagaa
     2161 aatgattaaa ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact
     2221 cgataaatac gatattccgg tggtggtgat tggcaaagtt gaaggtcaat atgcccatgt
     2281 ttattctgtc gataccgata attttggcga cagcattgcg ttgaccgatg cattaattga
     2341 aagtgggcat caaaatattg cctgcctgca tgctccgctt gatgtccatg tttcagtgga
     2401 tcgggtaaat ggttataagc agagcctggc tgcgcataat attgcagtgc gtgatgaatt
     2461 gattgttgac ggtggttata cccatgaaac agcactgcaa gccgcgagag aattattaag
     2521 ccagtcaccg ttgcctgagg cggtgtttgc cactgacagt ctgaaattaa tgagtattta
     2581 tcgtgcggca gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggttatag
     2641 taatgaaacg ctgtcgttta tattaacgcc tgcaccgggc ggcatcgatg ttccgacgca
     2701 ggagttaggt caacaaagct gcgagttatt attccgctta atttctggaa aaccgtcacc
     2761 acagaatatt accgttgcca cgcatatgac gctgaaataa ataccagcta ccttgcctga
     2821 atgccggatg cgatgctagc gcatcttatc cggcctacat ttatagatcg gataaagtac
     2881 tcgcgccgca tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc
     2941 ataatggaat ttatcactgt ctccttcgtc gtgatcctgc cactcaaacg cgtattccag
     3001 cgaaacggaa agtccgttct ggaaatcata accgtaaaat aaacctacac ggttaaaatc
     3061 atggccttca cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat
     3121 gcgcgtatac ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc
     3181 ttgcgtggaa aactcaccgt tattgcggct gtcgtccata ttgaagccgc gctcgagata
     3241 atagttcact cgcaaggcaa ccgtttcgtt gaaggtatat tgcagacctg tttccgtttc
     3301 gacacgggta tcagcgtaac cggtagtgtt cagatcgttg gcaaatttat acatcgacaa
     3361 ccaaccgttg aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg cgattttcca
     3421 gcgctgcata ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg
     3481 gaaaccgccg gtcaggccga aactgaaatc atcgttttcg aggaactgat aatgcacctc
     3541 cagctccggg cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc
     3601 ctgataatag gccagagcaa ttctccacgg cccgttggcg gcattaaaat agactgaagg
     3661 ctccgcc
//
LOCUS       DAJVXP010000232         3565 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVXP010000232 DAJVXP010000000
VERSION     DAJVXP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.236"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            754..1470
                     /gene="nanC"
                     /locus_tag="OQS10_004047"
     CDS             754..1470
                     /gene="nanC"
                     /locus_tag="OQS10_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS2294922.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1490..2596
                     /gene="nanM"
                     /locus_tag="OQS10_004048"
     CDS             1490..2596
                     /gene="nanM"
                     /locus_tag="OQS10_004048"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS2294923.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2661..>3565
                     /locus_tag="OQS10_004049"
     CDS             2661..>3565
                     /locus_tag="OQS10_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS2294924.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSH"
BASE COUNT         1078 a          739 c          801 g          947 t
ORIGIN      
        1 tcgacttcag aagcgaccag ttacaagcca caaatcagga aacatatttc atggtgagaa
       61 ttatgatcag cctgatggcg ggtaaacact tgttttcacc acgctcactg agttagcttg
      121 caaagcttcc cttgtttaga ccctaaataa attttttata aaacaaacag ttaaaagaaa
      181 tcctttagtt tttgaagacc acctcaagtg ttcgtctggc ttcacatggc atcttcctct
      241 tagaaaaaga tcgacatatt ttgtgacacg aattgcaaat ctcgttttgt tgtatggctt
      301 gcgtgatttt tgatctggta taacaggtat aaaggtgcac cgcgatagtc aatgagacag
      361 ggcatctcgc aatctatggc aaacatcact tcagttcttt ctcatcgggt gatgaaaacg
      421 cacttcagtc tgaaaggaat atgaaaatga gatcaacaga cactctattt tatgactctg
      481 ggtaaaatgg attgagtaag tgatatagct tacgaacatt caaatcaatt aaacatcaga
      541 agagatttta tactcaggta tttaatctga ttctctgttt atttaaataa tgtggaaaga
      601 gaattttcac aggagacctt atacaaaaat ataaaataag ctacccgttg ccaaagacac
      661 tataagcctg gcaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta
      721 ttgctttctg tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc
      781 gtattattac tgtgcttttc gtcaccatta atttctcagg ctgcgacact ggacgtacgt
      841 ggtggatatc gtagcggaag ccatgcctat gagactcgac tcaaagtcag tgagggatgg
      901 caaaatggat ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa
      961 aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta attacgcgat taaacttgat
     1021 gatcaatgga cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac
     1081 ggaccctacg taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat
     1141 cgttacgact ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac
     1201 gttcatcgtt gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg
     1261 caaacgacgc tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg
     1321 acggaaaatg cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa
     1381 tatgactacc ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt
     1441 tatcgcattg gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac
     1501 tataatggcg cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga
     1561 aactcctgtg ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg
     1621 tttaggtagc gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg
     1681 gactgcgtta gctgcattcc ctggtggacc gagagatcaa gcaacctctg catttattga
     1741 tggcaatcta tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt
     1801 taatgacgta cacaaataca accccaaaac caatagctgg gttaaattga tgtcgctcgc
     1861 gccgatgggc atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg
     1921 cggtgttaac cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga
     1981 ttcaaccgct atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt
     2041 cttcaataag tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga
     2101 atcgccctgg tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct
     2161 tattaatggc gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac
     2221 cggtaataat ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg
     2281 cggttttgcg gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg
     2341 ttcacgagaa aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata
     2401 tagcactgat attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca
     2461 aggtcgggcc tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga
     2521 aactgcaggc ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt
     2581 cacagtacaa aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat
     2641 ttgtattacg cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta
     2701 ccgttgctgg tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg
     2761 aagatgcgcc tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag
     2821 gcccgccatg tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg
     2881 atatgcaggg ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg
     2941 gccaggcact gcatattgca cggaaattac tgcccttcat tcctaataat gcagggattc
     3001 ttatcgttcc gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt
     3061 cagaacggca cggagccagc catgatgctt gtcgttgggg aacggatact ccgctgtacc
     3121 aggatttagt cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg
     3181 gcgtatgctg gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc
     3241 aacactttaa tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc
     3301 ttaataatat tactgacgcg ccatggtttt gcggcgatac cacctggtac tggaaagaaa
     3361 atttcccgca ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata
     3421 ttattttcgt cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag
     3481 acccggacga tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga
     3541 cgacggcact gcgaagcagt cattt
//
LOCUS       DAJVXP010000233         3559 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVXP010000233 DAJVXP010000000
VERSION     DAJVXP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.237"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            129..1376
                     /locus_tag="OQS10_004050"
     CDS             129..1376
                     /locus_tag="OQS10_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCS2294925.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="OQS10_004051"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="OQS10_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCS2294926.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="OQS10_004052"
     CDS             complement(2228..2572)
                     /locus_tag="OQS10_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294927.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="OQS10_004053"
     CDS             2814..3416
                     /locus_tag="OQS10_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294928.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAJVXP010000234         3547 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVXP010000234 DAJVXP010000000
VERSION     DAJVXP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.238"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..143)
                     /locus_tag="OQS10_004054"
     CDS             complement(<1..143)
                     /locus_tag="OQS10_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294929.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="OQS10_004055"
     CDS             complement(189..530)
                     /locus_tag="OQS10_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294930.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="OQS10_004056"
     CDS             complement(699..890)
                     /locus_tag="OQS10_004056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294931.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OQS10_004057"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OQS10_004057"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS2294932.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3547)
                     /gene="xylA"
                     /locus_tag="OQS10_004058"
     CDS             complement(2226..>3547)
                     /gene="xylA"
                     /locus_tag="OQS10_004058"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS2294933.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgca
//
LOCUS       DAJVXP010000235         3529 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVXP010000235 DAJVXP010000000
VERSION     DAJVXP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.239"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            31..759
                     /gene="mpaA"
                     /locus_tag="OQS10_004059"
     CDS             31..759
                     /gene="mpaA"
                     /locus_tag="OQS10_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS2294934.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(734..1699)
                     /gene="ycjG"
                     /locus_tag="OQS10_004060"
     CDS             complement(734..1699)
                     /gene="ycjG"
                     /locus_tag="OQS10_004060"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS2294935.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1818..2324
                     /gene="tpx"
                     /locus_tag="OQS10_004061"
     CDS             1818..2324
                     /gene="tpx"
                     /locus_tag="OQS10_004061"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS2294936.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2368..>3529)
                     /gene="tyrR"
                     /locus_tag="OQS10_004062"
     CDS             complement(2368..>3529)
                     /gene="tyrR"
                     /locus_tag="OQS10_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS2294937.1"
                     /translation="LINGFNFLRWLESEPQDSHNEHVVINGQNFLMEITPVYLQDEND
                     QHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVEQAQKLAMLSAP
                     LLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELFGHAPEGKKGFF
                     EQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVRVICVTQKNLVE
                     LVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQGVQRPKLAADL
                     NTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATVAVGEDAMEGSL
                     DEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQKKNEE"
BASE COUNT          817 a         1009 c          850 g          853 t
ORIGIN      
        1 cctgtaatac ttcctgaacc agagacgatc atgaccgtaa cccgcccacg cgccgaacgc
       61 ggcgcatttc cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc
      121 tggtttcctg cccctgcagc cagccgcgaa agtggtttga ttctggctgg cactcacggt
      181 gatgaaaact cttcggtcgt cacgctctcc tgtgctctgc ggacgttgac accttctttg
      241 cgccgccatc atgtggtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc
      301 aatgctaatg gtgtggattt aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa
      361 acggtttatc gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac
      421 aaacctggct cagaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct
      481 gcctgggtgg tctcattcca cgatccactg gcctgtattg aagatcccag acacagcgaa
      541 ttaggcgaat ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa
      601 acgcctggct ctttcggcag ttggtgtgca gatctcaact tgcattgcat caccgcagag
      661 tttccgccca tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg
      721 ctgcgctggc atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat
      781 ctaccgccag ccaggtcggt ccgtcaagat cggcgaaact gacctgcggc accagcggta
      841 aagcggcgct aatggcacga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt
      901 gtgcacgcgc ttcagtcgcc agcgccagcg cttccgtcag accaccgatt ttatcgagct
      961 taatgttaac catctcatag cgccctttca gcgccttcag attgctacgt gtatgacaac
     1021 tctcatcagc acaaatcggc aacggatgaa taaaattctc cagcgccgca tcatcctgcg
     1081 caggaagggg ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgcgccg
     1141 ccaccccttc tgcacgccag gattcatttg catcaacgat cagcgtcgca tcgggcacag
     1201 ctgtgcgaat tgccaccatc cgctcactaa taagatggtt atccagcttc actttcagta
     1261 atttcgcgcc tgcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga
     1321 tgactaccgt ctgtgcagtg ataactgtct cgggaagcgt tatgccgatc aaatcagcca
     1381 gcgattgctg ctgttttcgc gcggccagat cccacaatgc acaatccagc gcattacgtg
     1441 ctgcgccggc agggagaatt ttttgcaact cctcccgtgt cagccctttc tctaattgcg
     1501 acacgacgct cataatttgc gccattaccg aggcatcact ttctccataa cgcggatacg
     1561 gcgtgcattc gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg
     1621 cttcactgcg acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcaa
     1681 ataccttaac ggttctcatc tttactcctt gttgtcgcgg tggtctgcgg attaagcgca
     1741 aatagcgttt gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt
     1801 aaacaggaaa gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg
     1861 ccaattccat cccgcaggcg ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc
     1921 tgtctgacgt caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga
     1981 gtattgatac cgatgtttgc gccgcatcag tacgcaagtt taaccaactg gcaactgaga
     2041 tcgacaacac cgttgtgctg tgtatctctg ccgatctgcc gttcgcccag tctcgtttct
     2101 gcggcgcaga aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaatttc
     2161 tgcaagctta cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg
     2221 ttgtggttat tgacgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacca
     2281 ccgagccgga ttacgaagca gctctggctg tactgaaagc ataagttaat atgcctgatg
     2341 gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata
     2401 ttcccgcaac ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg
     2461 cgtgctggga taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat
     2521 ttcgtccagc gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc
     2581 cggcaacaaa atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata
     2641 gatagcgttc tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt
     2701 caggtcagcg gccagtttcg gacgctgcac gccctgctcg tcggcaaagc gggcgacgaa
     2761 cagctcagtt aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt
     2821 caacacgttc agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac
     2881 cagattcttc tgcgtaacgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc
     2941 aacccgacgg aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga
     3001 catttcccct atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt
     3061 cttcccttcc ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc
     3121 acagttcagc gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc
     3181 aaagagatct ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc
     3241 cagtttctgc gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact
     3301 gaaggcgctg acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga
     3361 tcgcaacatc accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac
     3421 aggcgtaatc tccatcagga aattctgccc attaataacg acatgctcgt tatgcgaatc
     3481 ttgcggttcg ctttccagcc aacgtaaaaa attaaagccg ttaatcaat
//
LOCUS       DAJVXP010000236         3485 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVXP010000236 DAJVXP010000000
VERSION     DAJVXP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.240"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            63..1226
                     /locus_tag="OQS10_004063"
     CDS             63..1226
                     /locus_tag="OQS10_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS2294938.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OQS10_004064"
     CDS             complement(1686..2123)
                     /locus_tag="OQS10_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294939.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3485)
                     /locus_tag="OQS10_004065"
     CDS             complement(2212..>3485)
                     /locus_tag="OQS10_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCS2294940.1"
                     /translation="DSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLT
                     GWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQR
                     RSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQM
                     QNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNLLNEENPHQLQQ
                     LIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPITDIDPL
                     GLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRG
                     PIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFC
                     SEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          866 a          814 c          811 g          994 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaattg ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc tccaccgtca ggccgcacga tgccagccag cggcggcttt cctcactcac
     3061 ccggtcagcc aggatttcac tttccagccg gtcgagcatc tgcaccagca ccggcgggaa
     3121 caccacactg ccaccgtctt cgccgccgga ctgctgaagg gcatccgcca ggctgcggcg
     3181 ctgcgttttc gccagctcac cggtggcagt ttcgaccctg atgagtggcg tgaagctccc
     3241 cggctgataa atcgtctgga tgcgggttct gtcgttctgt attgtggtca gccggtcgcc
     3301 gtcccagccg taccaggtca cttgcggttt ccgtgacagc gacatccagc ccgtcaggtc
     3361 ccgttcacgc cgccataccc gttttgccac cctgcggccc agcgggtcgt aaagatagcg
     3421 actttcgacc agcggctctg catattgtgt ccgcgtgtag tgcaccagcc ggtgctgact
     3481 gtcgt
//
LOCUS       DAJVXP010000237         3369 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVXP010000237 DAJVXP010000000
VERSION     DAJVXP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.241"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            58..315
                     /locus_tag="OQS10_004066"
     CDS             58..315
                     /locus_tag="OQS10_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCS2294941.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(365..1315)
                     /gene="uspE"
                     /locus_tag="OQS10_004067"
     CDS             complement(365..1315)
                     /gene="uspE"
                     /locus_tag="OQS10_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS2294942.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1467..2219)
                     /gene="fnr"
                     /locus_tag="OQS10_004068"
     CDS             complement(1467..2219)
                     /gene="fnr"
                     /locus_tag="OQS10_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS2294943.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2414..2929)
                     /gene="ogt"
                     /locus_tag="OQS10_004069"
     CDS             complement(2414..2929)
                     /gene="ogt"
                     /locus_tag="OQS10_004069"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS2294944.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2940..>3369)
                     /locus_tag="OQS10_004070"
     CDS             complement(2940..>3369)
                     /locus_tag="OQS10_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCS2294945.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT          881 a          811 c          818 g          859 t
ORIGIN      
        1 tgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga ttacatcatg
       61 attatggcga aactgaagtc agcgaaaggg aaaaaatttc tctttggttt gttggcggtt
      121 ttcattattg cggcgtcggt tgtgactcgc gcgactatcg gcggcgttat agaacagtac
      181 aatattccgc tgtctgagtg gacgacatca atgtatgtga ttcagtcatc gatgattttt
      241 gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta tttccttggc
      301 ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag ttattgaaga
      361 gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg tcaggtttaa
      421 tgaccaacag atcgcagcga agatgatcaa tcacctgttc cgccgtgttg ccgaggaatg
      481 ctgctgaaat accggtgcgt cctaccgtgc ccagaaccac aatccccgcc tgtaagtgct
      541 ccgccaaatc aggaataacc tcttccggca gacctttttc tacgtgcgtc atgttttcat
      601 taatgccgaa tttctgccgc agagctttca ttgccagcaa atgttgccca cgaatggcat
      661 cgttataaac gctcggatca aattccggca gttcaatcgc gatattaatt ggcgttactg
      721 ggtaagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt tcgatcgtct
      781 ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg agattcaccg
      841 ccaccagcgc cttacctcct tccggccacg gctggtcttt caccatccac accgggcttg
      901 ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc agacggtcat
      961 gttggtgcgc catttttagc accaaatcgt gcccgccgct gatcacttcc tgaatgatgg
     1021 cttcgaaggg acggttatgc cagaccactt taatttcaat gggaacgcca gcattgagat
     1081 aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc tgacgcatag
     1141 cggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca tagatcggca
     1201 aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc cgcaatgctg
     1261 gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata gccatacagg
     1321 gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca tatctggtga
     1381 atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa tgatatgaca
     1441 gaaggatagt gagttatgcg gaaaaatcag gcaacattac gcgtatgacc agcaagctgg
     1501 gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag cataccgctt
     1561 ttctggaagc gacctagcag acggctgatg gtttctaccg tcaggcccag atagttaccg
     1621 atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg ttgggcaaaa
     1681 cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt cttcgacaac
     1741 agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat catctgctga
     1801 cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacgggat ttcacatacc
     1861 atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat ggcgtcaaat
     1921 cccaccaggt cgccagctaa atggaaacca gtgatttgct cgtcgccttg ctcagtgatg
     1981 gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc atcaccagcc
     2041 ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt atcaagctga
     2101 tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct gcaatcctgg
     2161 caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc cgggatcata
     2221 ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg gagcaagtaa
     2281 gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata tgtctatcta
     2341 tttgaaaacc cttaagttgt taagggtaac tttacataaa agtgtgaaca agctggcaca
     2401 aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac tcttttcgct
     2461 gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga tggcaaggta
     2521 cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc gcgccagggc
     2581 ggcccaattg ttcagccagt tggccgtaat gcattacctg tccgcagggg atagtgcgta
     2641 gagttttcca gacttcgcgc tgaaatggcg tcccccctgt ggcagtggga agcgtatcaa
     2701 taatgctaag attaccggca aaataatcac gaagcttgtc gcttaaaccg cctgggttgg
     2761 tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc tgcaccatgc
     2821 gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg caaatcaccc
     2881 acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc cgttctctct
     2941 taagacaaac gtgggtaaat ccccggacct atcggcagac caacaagata ccacgccagc
     3001 aacatcagca gccataccac caaaaagata agcggatagg gcaagactaa cgaatagtaa
     3061 gtacccagtt tcgcgtctgg tttgtagcgt tgcaggaatc caagaaacag tggaacaaaa
     3121 ggagataccg gcgctaaagg caatacggat gagtcggcaa tacgaaagag gatttgcgca
     3181 aatgccgggt gaaagccaag tagcataaac attggtacga aaatgggggc cagaatcgac
     3241 cagattgcgg aaccgctggc aataaacatg cataagaaag aggaaagcaa cgccagaccg
     3301 acaaacgccg ggatgccgct aagccctgaa ctttccagta tatcggtcag ccccacggcg
     3361 atgaatttc
//
LOCUS       DAJVXP010000238         3325 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVXP010000238 DAJVXP010000000
VERSION     DAJVXP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.242"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..416
                     /gene="adeQ"
                     /locus_tag="OQS10_004071"
                     /gene_synonym="yicO"
     CDS             <1..416
                     /gene="adeQ"
                     /locus_tag="OQS10_004071"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS2294946.1"
                     /translation="FIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLV
                     AMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGF
                     MSYCIMKVCTGRWRDLNLCVVVVASLFALKIILVD"
     gene            469..921
                     /locus_tag="OQS10_004072"
     CDS             469..921
                     /locus_tag="OQS10_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS2294947.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1132..2312
                     /gene="nepI"
                     /locus_tag="OQS10_004073"
                     /pseudo
     CDS             1132..2312
                     /gene="nepI"
                     /locus_tag="OQS10_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2353..2646)
                     /locus_tag="OQS10_004074"
     CDS             complement(2353..2646)
                     /locus_tag="OQS10_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294948.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            2868..>3325
                     /locus_tag="OQS10_004075"
     CDS             2868..>3325
                     /locus_tag="OQS10_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HCS2294949.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQK"
BASE COUNT          707 a          768 c          886 g          964 t
ORIGIN      
        1 cgtttatcgg cacctcgtct gttactgcct atattgaaag tacttctggt gtggcagtcg
       61 gtggccgcac ggggctgact gcggttgtgg ttggcgttat gttcctgttg gttatgttct
      121 tctccccgct ggtggcgatg gttcctcctt acgcaaccgc cggagcgtta atctttgttg
      181 gcgtgctgat gacttcgagc ctggcgcgcg ttaactggga tgattttacc gaatcggtgc
      241 ctgcgtttat taccacggtg atgatgccct ttactttctc gatcaccgaa gggattgcac
      301 tcggctttat gtcgtactgc atcatgaaag tgtgcaccgg gcgctggcgc gatctgaacc
      361 tgtgtgtggt ggtggtcgca tctctgtttg cactgaagat tattctggtg gattagtaac
      421 tggcgagggt ttccagatat catgagttct gataacgcag gagaactcat gatctggata
      481 atgctcgcca cgctggcggt agtgtttgtg gttggttttc gggtgctgac atccggggcc
      541 agaaaagcga ttcgccgtct cagtgatcgg ctgaatatcg atgtcgtacc cgtggagtcg
      601 atggtcgatc aaatgggaaa gtcagccggt gacgaatttt tacgttattt gcatcgcccg
      661 gatgagtcgc acctgcaaaa cgccgcgcag gtgttgctca tctggcaaat tgtcattgtc
      721 gatggtagcg aacagaacct gctgcaatgg catcggattt tacaaaaagc ccgcctttcc
      781 gcgccgatta ccgatgctca ggtcaggctg gcgctgggtt ttctgcgcga aaccgaacct
      841 gaaatgcagg atattaatgc ttttcagatg cgctataacg cgttctttca gcctgccgag
      901 ggcgttcact ggctgcatta aaaccgcttc gtcttatcag cctacagacc gcgcctgacc
      961 tgtcgggtgg ataagacgtt cacgccgcat ccggtaatct ttgccctgtt ccgtttcgat
     1021 cttaactcca gctcaagcaa caccgtaaaa gttgcaaaaa cgttaaatgc gtcacacatt
     1081 tcaatgttaa agtggtcggc ttttcccctg aaacatgcca cgggtaacac catgagtgaa
     1141 tttattgccg aaaaccgcgg cgcggatgcc atcacccgac cgaactggtc agccgttttc
     1201 tcggtggcgt tttgtgtcgc ctgtctgatt atcgttgagt ttttgcccgt cagtttgttg
     1261 acgccaatgg cccaggattt aggcatttcg gaaggggttg ccgggcaatc ggtgaccgtg
     1321 accgcctttg tggcaatgtt tgccagtttg tttattaccc agacaattca ggctactgac
     1381 cgccgcaacg ttgttatttt gtttgccgtt ttgctgacgc tctcctgctt gctggtttcc
     1441 tttgctaact cattcagttt gcttttaatc ggtcgtgcct gtctggggct ggcgctgggc
     1501 gggttctggg cgatgtcggc gtcgctgacc atgcgtctgg tgccgccgcg tacggtgccg
     1561 aaggcgctgt cggtgatctt cggcgcggtt tctattgcgc tggtgattgc cgcgccgttg
     1621 ggcagttttt taggcgagct tatcggttgg cgcaatgtct ttaatgcggc ggcggtgatg
     1681 ggcgtgctgt gtattttctg gattatcaaa tcattgcctt cactgccagg cgaaccctcg
     1741 catcagaaac aaaatacttt ccgcttatta caacgtccgg gtgtgatggc agggatgatc
     1801 gccatcttca tgtctttcgc cgggcagttt gctttcttca catatattcg cccggtgtat
     1861 atgaacctgg cgggattcag cgtggatggc ttaacgctgg tgctgttgag ttttggcatt
     1921 gccagcttta tcggtacgtc gctttcgtca ttcattctta aacgttcggt aaaactggcc
     1981 ttagcaggcg cgccgttaat actggctgtg agtgcgttgg tgctgacgtt gtggggaagc
     2041 gataaaatcg ttgctaccgg cgtggcgatt atctgggggc taacttttgc attggttccc
     2101 gtcggctggt caacgtggat cacccgctca ctggccgatc aggcagaaaa agccgggtct
     2161 attcaggtgg cggttattca gcttgtggcg cggcaatcgg cggttatgcg ctggataata
     2221 ttggtctgac ttcgccgttg atgttttctg gcacattgat gttgctgact gcattgttgg
     2281 ttactgcaaa ggtgaaaatg aagaaatcct gatatcaggt aaagaagcgt tggtcagtag
     2341 ttgttgctgt tcttacatat ccgggcattc taccttaccc aacgcttccc aataattgct
     2401 ctgattatta cgttccatcg ccacgatggc ctcgcgtata tgttgtttat tgttttcatc
     2461 tgacaacatt aacttttccc acgcttcatc tgataatgac gcctgtggcg tgcaaatttt
     2521 acgtaaatct tgtaataccg tggttttctc ttttgcggac tgcagtgagc taaaaggcga
     2581 ctcggcgtat acgatgcctg gcatagcgaa aaaagtgaaa ataagcagta gtgtcgttgg
     2641 tttcatggat tcaacctttt acaaatgtgt atcgaatgaa gcataagtgt agtttgcttt
     2701 catcgctctc atcatgctgt acgaaacgcg cttgcgtggt cagtaagaag tgccagactt
     2761 tatattccac ttttattcct ttttattctt atcgatagcg tttcgttttt taaaccgcag
     2821 cgaccttacc gctatagtca ggtaatcatt aataaaagga taaaaaaatg aaactgacaa
     2881 cacatcatct acggacaggg gccgcattat tgctggccgg aattctgctg gcaggttgcg
     2941 accagagtag cagcgatgca aaacacatta aagttggcgt tataaatggc gcagaacaag
     3001 atgtcgcgga agtcgctaaa aaggtggcaa aagagaaata tggcctcgat gttgaactgg
     3061 taggatttag cggttcgttg ctaccgaacg atgcgacaaa tcatggcgaa cttgatgcga
     3121 atgtcttcca gcatcgaccc tttcttgaac aagataatca ggcgcatggt tataaactgg
     3181 tagcgctggg aaatacgttt gtcttcccta tggcgggtta ttccaaaaaa atcaaaaccg
     3241 tggcgcaaat aaaagaaggc gcgacagtgg cgatccccaa cgacccgacc aaccttggtc
     3301 gcgcactttt actgttgcaa aaaga
//
LOCUS       DAJVXP010000239         3318 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVXP010000239 DAJVXP010000000
VERSION     DAJVXP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.243"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..626
                     /locus_tag="OQS10_004076"
                     /pseudo
     CDS             1..626
                     /locus_tag="OQS10_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(743..2086)
                     /gene="gdhA"
                     /locus_tag="OQS10_004077"
     CDS             complement(743..2086)
                     /gene="gdhA"
                     /locus_tag="OQS10_004077"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS2294950.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2322..2594
                     /locus_tag="OQS10_004078"
     CDS             2322..2594
                     /locus_tag="OQS10_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS2294951.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2560..2967)
                     /gene="nudG"
                     /locus_tag="OQS10_004079"
     CDS             complement(2560..2967)
                     /gene="nudG"
                     /locus_tag="OQS10_004079"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS2294952.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            3054..>3318
                     /locus_tag="OQS10_004080"
     CDS             3054..>3318
                     /locus_tag="OQS10_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS2294953.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          828 a          839 c          817 g          834 t
ORIGIN      
        1 cgaaagcctg aacctggtaa agataccttc caatatcatg tgcttccttt tgactcgatc
       61 gatatcatca gtaaaagacg cgagtcttta gagaatcaat ggggtatcga agatagcgaa
      121 agttattgtg cctaatggag cattttcttt ctggcgacca tggagccaat acctttaaag
      181 caaacatgga ggaagcccca gagcaggtta tcgccttgtt gaataaattt gctgtttttc
      241 cctcagacta tatctctgat tgcgctaata atcgctccgg taaatcctcg gcgaagctaa
      301 tatgggcggc ggaattatca tggatgacct cgatatcaag cacagctttt caaaacggga
      361 caattgaaga agaactggcc tggcattata taatgcttgc ttctcgaaag gcgcacgagt
      421 tgttcgaaag cgaagaagat tatcaaaaaa atagtctaat ggggtttctt tactggcata
      481 tctgctgcta tcgcagaaag ttaacggatg ctgaacttga agcgtgttat cgttacgaca
      541 agcaattttg ggagcactac agtaagaaat gccgttggcc cataagaaat gttccctggg
      601 gagcatcatc cgttaaatac tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc
      661 ccggcctaca ttagagcgta actgcaatga attgtgccca tttgtaggcc tgataagcgt
      721 agcgccatca ggcatttaca acttaaatca caccctgcgc cagcatcgca tcggcaacct
      781 tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt ttgctcacct tcaccaccat
      841 gctcaacaca ggcatggtgg atatccagca tgatgtgatg caaacgtgcg tcaactttct
      901 cggctttcca gcccaggcgc gcagcgtttt gtgccatttc caggcccgac gttgctacac
      961 cgccagcatt agccgcttta cccggtgcaa acagtacgcc tgcctgctgg aacagttcag
     1021 tcgcttcgat ggtggtcggc atatttgccc cttcagcgac ggctttaacg ccattggcga
     1081 taagctgatg cgcggcgtca acatccagtt cgttctgggt ggcgcaaggc agggcgatat
     1141 caaccggtac agaccacggc tgttggcctt cgagatagac cagaccaaat tctttggcgt
     1201 aatctgccac tcgaccatcg cggctggctt tgatttcgat aagacgtgcc agtttctctt
     1261 tcgtgaatcc gctttcatca actacagtgc cgctggagtc tgacgcagtg atcacacgag
     1321 caccaaattc catcgctttt tcgatagcgt actgggcgac gttgccggag ccagaaacgg
     1381 aaacgcgcat cccttcaaaa cccataccgt ggcgttttaa cattgcttct gtgaaataaa
     1441 ccagaccgta gccggtagct tccgggcgaa taagactacc gccaaatgaa aggcccttac
     1501 cggtgaagac gcaggcggta ttgttggaga gctttttcat catccccgcc ataaagccga
     1561 cttcacgacc accaacgccg atatcacctg ccggaacgtc ggtatccgcg cccagatgac
     1621 gataaagctc ggtcatcagc gcctggcaaa aacgcatcac ttcaccttcg ctttttcctt
     1681 tcggatcgaa atcgctgccg cctttaccac cgcccatcgg cagggtagtc agggcatttt
     1741 tgaaggtttg ttcaaagccg aggaatttga gaatggaaag gttaactgac ggatggaagc
     1801 gcataccgcc tttgtacggg ccgatggcag agctgaactg cacacgccat gcacggttga
     1861 cctgtatctg gttgcgatca tcaacccata ccacgcgaaa ctggatcacg cgctccggtt
     1921 caaccagacg ctccagtaat gacatctggc gatattttgg attttgttca agaaaaggcc
     1981 agagtgtggt cattacttca cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct
     2041 tttggacatg gttgaggaat gactccagag aatatgtctg atccatagat ataaaaccct
     2101 tatatattaa tacgattgtg cttatgttgt ttatgtcatg tgcttttgca gttttcgact
     2161 ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac ccgggaaagc aaattaataa
     2221 cgagagtaat ctcataacca aatgcgataa ctcagacgct aaagttttgc cacccggcta
     2281 ccgttatcag ttgcagagac aataacgtta aggagttccc tgtgagtcga gcattgttcg
     2341 ccgtagtatt agcatttcca ttaattgcac tggctaaccc gcattatcgt ccggacgtgg
     2401 aggtaaacgt accgccagaa gtgttcagtt caggcgggca gtcagcgcag ccctgtactc
     2461 agtgctgcgt ttatcaggat caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa
     2521 tactgctgca atgccagcgg gatgacaaaa cccttagcac taatccgctg ctctggcggc
     2581 gcgtaaagcc ataaacgcct ctaatagtgg aatgtcagca ggggccagcg gatattgcag
     2641 cgcctcttca ggtgagcacc agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg
     2701 gaagtcgggt acgtgccagg catgaagatg gataatccgg cccgaaactt ctcgctgatg
     2761 gctggcaaca tactcaccca cagttgcttc gatgcccaat tcttcgttta actcacgcac
     2821 cagtgcctgc tgctggcttt catccagctc gactttacca tcggcaaact cccataatcc
     2881 cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat
     2941 ggcggcaaca acttcaatca ttttcataaa ttacacacgg gcagataagc gaaaaatgcc
     3001 ataatatccg cactgttgct caatcagaaa ggctttatat ttaaggagtg cctgtgctag
     3061 accgccatct tcatccccgg attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata
     3121 aaccgggcat tacgccagat ggtttaacat tagtgggatt tgccatcggc gtgctggctc
     3181 tgccgtttct ggcgctgggc tggtatctgg cggcgctagt cgtcattttg ttgaacaggc
     3241 tgcttgatgg tctggacggt gcgctggcgc ggcgcagaga gcttaccgac gcgggcggct
     3301 ttcttgatat ttctcttg
//
LOCUS       DAJVXP010000240         3313 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVXP010000240 DAJVXP010000000
VERSION     DAJVXP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.244"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..73)
                     /locus_tag="OQS10_004081"
     CDS             complement(<1..73)
                     /locus_tag="OQS10_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein HspQ"
                     /protein_id="HCS2294954.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVV"
     gene            complement(131..1321)
                     /gene="rlmI"
                     /locus_tag="OQS10_004082"
                     /pseudo
     CDS             complement(131..1321)
                     /gene="rlmI"
                     /locus_tag="OQS10_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            1416..1694
                     /gene="yccX"
                     /locus_tag="OQS10_004083"
     CDS             1416..1694
                     /gene="yccX"
                     /locus_tag="OQS10_004083"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS2294955.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(1691..2020)
                     /gene="tusE"
                     /locus_tag="OQS10_004084"
     CDS             complement(1691..2020)
                     /gene="tusE"
                     /locus_tag="OQS10_004084"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS2294956.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2111..2770)
                     /gene="yccA"
                     /locus_tag="OQS10_004085"
     CDS             complement(2111..2770)
                     /gene="yccA"
                     /locus_tag="OQS10_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS2294957.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(2977..3064)
                     /locus_tag="OQS10_004086"
     tRNA            complement(2977..3064)
                     /locus_tag="OQS10_004086"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3028..3030),aa:Ser,seq:tga)
BASE COUNT          910 a          847 c          784 g          772 t
ORIGIN      
        1 ccacgaccac tccgagataa cctaacaggg aatggcggac ctgctggccg ataccgaatt
       61 tgctggcaat catagtcacc tcccgggaaa tctttacctt tgatataagg gtaaaattcc
      121 acatttcaag ttacatgacg cgacaggcaa accctttcag atatagccct tccggatagg
      181 tagcgatcac cggatgatcg gctgcctgac ggaactgctc tataaattgt acatcacggc
      241 cggcatcaat tgcggcatcc gcgatgattt tctgaaataa atcgctggtc atcagaccgg
      301 aacaggagaa agtcaggaga ataccgcctt cattcagcaa ctgaatcgcc agcatgttga
      361 tatctttata gccacgacac gcgcccatca actggctttt attctcaaca aacttcggcg
      421 ggtccatcac gataacgtca aatttttcac cgcgatcgcg ataagtacgc agcaatttaa
      481 agacatcatc acggacaaac tcagccttgc tcagatccag tttgttcagc tcaacgttct
      541 gccgtgcaat atccagcgct tcctaggagg tatcaacgct gacaacctgg ctgcaaccgc
      601 ccatcagtgc cgatacggcg aaaccaccgg tataggagaa acagttcagc acacgtttat
      661 tttcaacgta gcggcgggta gccaggcggc tgtctcgctg gtccaggtag tagcccgttt
      721 tgtgtccgtg ctgaatatcc accagcagct tcatgccgtg ttcttcaatc ggcagcaggg
      781 caggcggcaa ctcgccggtg acgaggccct gggtcagctc catcccttct tttttacgta
      841 ccgcaacatc gctgcgatcg taaatcgcac attccgggta cagcgtttgc agggcactaa
      901 ttaatgccgc gcgctgatat tctgccccag cactcagcag ttgcagcacc agaaaattac
      961 cgaaacgatc gatagtaata cccggcaggc catcagattc tccggcgatt aaacgatagc
     1021 tgtcgaggcc atctttttgc gccagtcagt cacgccattt ttgtgcttgt tgcaaacggc
     1081 gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa cgtccagacg cgcgcccgga
     1141 tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt tccctgatga tcaacaatat
     1201 cgatggtttc accgaggctg gctttacctt ccatgcgggc aacggccccg gaaaagaccc
     1261 acggatggcg acgaagtaat gatttttcgc gccctttggc taacactaaa cgtacactca
     1321 taatttactt ttctgtcgat gcaaagaaat gggcgtcatt gtccggagtt ttccatggat
     1381 ttgcaacgcg tccattaagg ataaggagag cagcaatgtc gaaagtctgc ataattgcct
     1441 gggtttacgg gcgagttcag ggcgtaggat ttcgctacac cacacagtac gaagcgaaaa
     1501 gactgggttt aaccgggtac gccaaaaatc ttgacgatgg cagcgtggaa gtggttgcct
     1561 gcggtgaaga agggcaggtg gaaaaattaa tgcagtggtt aaagagtggc ggcccgcgtt
     1621 ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc ctcgggggaa ttaaccgatt
     1681 ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc agcaattttg gtggcttgct
     1741 tagccggacc tttcggaaac agtcggtata aatagcggct gttacctttt tcttcgccaa
     1801 atttattcgc catcgctttt accagcatac gaatcgccgg agaagtattg aattccagat
     1861 agaaatcacg cacaaaacgc accacttccc agtgttccgg tgacagcgaa atcccttcgt
     1921 tctctgcaat caccactgcc agcggctcac tccactggct gctttctttg agatagcctt
     1981 cggtatccgt ttctatctct ttaccttcga agatcagcat aattattact accttaatca
     2041 gactggcggc agtgtaacaa aaaacaaagc cccgcataaa gcgaggctat gaaagtgtta
     2101 gcgggtgaga ttaatcgcgg ctggcgaagc ccagaatgct cagcaggctg acgaagatgt
     2161 tgtacagcga aacatacagg ctaaccgtgg cacgaatata gttcgtttca ccgccatgaa
     2221 cgatgttgct ggtttcaaac aaaatagcgc cagaggagat cagaatgaag accgcgctga
     2281 tcgccagatg cagagcaggc agctgcagga agatattagc aaccatacca atcagcacca
     2341 ccacaatacc cgccatcagc ataccgccga ggaacgacat atctttgcgg gtggtcagca
     2401 catatgcaga gcagcagaag aacactaacg ccgttccgcc cagtgccata gcgattacgt
     2461 cacccattcc ggcagacaga taggtgttca gaataggtcc gaggatataa cccagaaaac
     2521 cggtaaaggc gaatgcggag ataatcccgg tcggcttatt cgccgtttta taggtcagga
     2581 acatcaaacc atacataccc accagcgtca gaatcagacc cggagatggc agcatcagca
     2641 ccgtactggc agttgcggta atcgccgaaa aggccagcgt caggctcagc agaaaatagg
     2701 tattacgcag caccttatgg gtgctaagca gtgatgtacg gtcatgtgaa gaactaacaa
     2761 tacgatccat tgagtcactc tctatgacag atgtaattaa ttaagcagca taatgataat
     2821 gcgtaagggc acccagaagt tttacccatc tttacgcatt tgatctggaa caggtttaac
     2881 agcggattat caggtcatta agcaaatata acgccctgag aatttcgaca ggcaaaagaa
     2941 aaaggggtta gcatttagct aaccccttat cttatttggc ggaagcgcag agattcgaac
     3001 tctggaaccc tttcgggtcg ccggttttca agaccggtgc cttcaaccgc tcggccacac
     3061 ttccggaatg acgcgcacta taaacatccc gatgcggcgt gtaaacccct aatttgtttg
     3121 tttgcctgaa aaacagccaa aagtgcattg atagcgtgaa ataacagcag attgatcatt
     3181 tcatcaccat gaattccttc tcttttactc gtttagcaac cggctaaaca tccccaccgc
     3241 ccggcaaaaa gaaaaatagg tccattttta tcgctaaaag ataaatccac acagtttgta
     3301 ttgttttgtg caa
//
LOCUS       DAJVXP010000241         3287 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVXP010000241 DAJVXP010000000
VERSION     DAJVXP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.245"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..231)
                     /locus_tag="OQS10_004087"
     CDS             complement(<1..231)
                     /locus_tag="OQS10_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294958.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKL"
     gene            405..1118
                     /locus_tag="OQS10_004088"
     CDS             405..1118
                     /locus_tag="OQS10_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS2294959.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1150..1908
                     /locus_tag="OQS10_004089"
     CDS             1150..1908
                     /locus_tag="OQS10_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294960.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1954..>3287
                     /locus_tag="OQS10_004090"
     CDS             1954..>3287
                     /locus_tag="OQS10_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS2294961.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          752 a          791 c          853 g          891 t
ORIGIN      
        1 caattttttt gctgttttca cctgatttcg ttcaattgtt ttatgtaagg attttcccat
       61 caacggtaat tttgtcactg cacgaccgac atttccggcc tgttttccta aatgaactct
      121 ttcggcagca ccatattttt cgacaacggt aataagatgt tcacacaccg ttgacttgcc
      181 tgaaccatcg ctaccaataa cggcaattaa tggaggtgtt attgactgca tgaatatatc
      241 ctttaattta aacatccatt aaaaatattt atttggttaa tatgttttta tgaaagcgta
      301 attcaggtca atgtcacaat taaccattgt cacaataagg ttgaacggat attctgagca
      361 cggaccacgt aatattcaat acattattta ctgccgggtt tttcgtgaat caacgtaata
      421 tgtcaataat taattccacg ccagtgcgtg ttattgccat tgtaggatgt gatggttcag
      481 gtaaatcgcc cctcacggca agcctggtaa atgaactggc agcaagaatg ccaacagaac
      541 acatttatct cgggcaatcg tccgggctaa ttggcgaatg gatttcacag ctccctgtta
      601 ttggcgcacc ttttgggcgt tatctgcgaa gtaaagcggc acatgtgcac gaaaaaccct
      661 caacaccgcc tggcaatatt actgctctgg ttatctatct gctttcctgc tggcgggcgt
      721 acaagtttcg caaaatgttg tgtaaaagcc agcaaggctt tctgctcatc accgaccgct
      781 acccgcaggt tgaagtgccg ggttttcgct ttgatggccc gcaattggca aaaaccacgg
      841 gcggtaacgg ttggataaaa atgttaaggc agcgcgagct gaagctgtac caatggatgg
      901 catcttattt gcccgtattg ttgattcgtc ttggcattga tgaacaaacc gcgtttgcgc
      961 gtaaacctga ccaccaactg gcagcgttac aggaaaaaat cgccgttacg ccgcaactga
     1021 ctttcaacgg cgccaggatc cttgaactgg atggtcggca accagccgat gaaatcctgc
     1081 aagcgtcact acgtgcaatt cacgccgcgc tttcctgatc accaaaatgg ataggggatg
     1141 atttaacgaa tggatgcact acaaacacaa actgttaata gtacaaccgc gccgcagccc
     1201 agctacattc cggggctaat tgcggtggtc gggtgcgatg gcaccggtaa atccacgctg
     1261 accaccgatc tggtgaaatc attgcaacag cactggcaaa ccgagcggcg ctatctgggg
     1321 ctgctctcgg gcgaagacgg cgacaaaatc aaacgattac cgttggttgg cgtctggctg
     1381 gaacggcgac tggcggccaa atcctcgaaa acccaaagca tgaaaaccaa atctccggcg
     1441 ctatgggcgg cggtgattat gtactgctta tcgctgcgaa gaatggcgaa tctacgcaag
     1501 gttcagcgac tggcgcaaag tggcgttctg gtggtcagcg atcgcttccc gcaggctgaa
     1561 atttcgggct tttattacga cggaccgggg attggcgtcg aacgtgcgac cgggaaaatc
     1621 agcatgtttc tggcgcagcg cgaacggcgt ttataccaac aaatggcgca atatcgcccg
     1681 gaattaatta ttcgcctggg cattgatatt gagactgcca tctcccgcaa gcctgaccat
     1741 gactatgccg agctgcagga caaaatcggt gtcatgtcga agattggcta taacggcaca
     1801 aaaattcttg agattgattc cagagcgccc tacagcgaag tgctggaaca ggcacagaag
     1861 gcggtttctc tggttgccat cgtttctgac cgccgaagtt taacttaagg ctggcgggat
     1921 actttttact atcaacgata aggtcaggct gaattggcgg gttttaacat caaacattgg
     1981 tttgcagatg gcgcgtttcg caccattatt cgcaatagcg cctggttagg ctccagtaat
     2041 gtcgtgagcg ccttgctggg gctgttggcg ctctcgtgtg ccggtaaagg gatgacgccc
     2101 gccatgtttg gcgtactggt gattgtgcaa tcgtacgcca agtcgatcag cgattttatt
     2161 aagtttcaga catggcagct ggtggttcag tacggaacgc cagccttgac caacaataat
     2221 ccgcagcaat ttcgcaatgt cgtctcattt tccttctcgc tggatatcgt cagcggcgcg
     2281 gtggcgattg tcggcggcat tgccttactg ccattccttt cccattcatt aggtctggat
     2341 gaccaaagct tttggctggc ggcgctgtat tgcacgctca ttccttcaat ggcttcctcc
     2401 acgccgaccg gcattctgcg tgcggtagat cgcttcgatt taattgctgt acagcaggcg
     2461 acgaaacctt ttctgcgcgc agcggggagc gtcgtagcct ggtattttga ctttggtttt
     2521 gcgggttttg ttattgcctg gtacgtatcg aatctggttg gcggcaccat gtactggtgg
     2581 tttgcagcgc gcgaattacg ccgcagaaat atccataatg ccttcaaatt gaatctgttt
     2641 gagtctgccc gacacatcaa aggcgcgtgg agttttgtct ggtcaaccaa cattgcccac
     2701 tccatctggt cggcgcgtaa ctcgtgcagc acagtgctgg tggggatcgt gttaggaccc
     2761 gctgccgccg ggttatttaa aatcgccatg acattctttg acgccgccgg aacgccagcg
     2821 ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt tagatccgcg caccaccaga
     2881 ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg gaatcggtat tctggtggcg
     2941 ctcgcggtgt tgatcgccgg caagccgctc atttcgctgg tgtttggcgt taagtatctc
     3001 gaagcgtatg acctgattca ggtgatgctg ggcgcaattg tgatttcgat gctgggtttc
     3061 ccgcaggagt cattgctatt gatggcggga aaacagcgcg cttttctcgt ggcgcaaacc
     3121 atcgcctcaa tcggctacat cgtattgctg ttcatgttct gtcatctgtt tggtgtgctg
     3181 ggagcagcgt ttgcttactt cggtgggcaa tgtctggatg tggtgctctc gttaattcca
     3241 actttaaagg cattttttca gcgtcattcc ttgctttata acgcagc
//
LOCUS       DAJVXP010000242         3225 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVXP010000242 DAJVXP010000000
VERSION     DAJVXP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.246"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(79..606)
                     /gene="mobB"
                     /locus_tag="OQS10_004091"
     CDS             complement(79..606)
                     /gene="mobB"
                     /locus_tag="OQS10_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS2294962.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(588..1172)
                     /gene="mobA"
                     /locus_tag="OQS10_004092"
     CDS             complement(588..1172)
                     /gene="mobA"
                     /locus_tag="OQS10_004092"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS2294963.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1242..1511
                     /locus_tag="OQS10_004093"
     CDS             1242..1511
                     /locus_tag="OQS10_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS2294964.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1588..2574
                     /gene="srkA"
                     /locus_tag="OQS10_004094"
     CDS             1588..2574
                     /gene="srkA"
                     /locus_tag="OQS10_004094"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS2294965.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2591..3217
                     /gene="dsbA"
                     /locus_tag="OQS10_004095"
     CDS             2591..3217
                     /gene="dsbA"
                     /locus_tag="OQS10_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS2294966.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          823 a          777 c          784 g          841 t
ORIGIN      
        1 gacggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacac tggcgcgtct
       61 tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca caaaatcagc
      121 cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg gcacatcact
      181 ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc cggctccatc
      241 gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca gaatcaaatc
      301 cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt cttcgtctgg
      361 tttttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg ccgcgccagc
      421 cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat ggtgcgtatg
      481 cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt ttttcaaaag
      541 cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca tcgtttttcc
      601 tgccatcggg caagctcctc tggcgtattc acgttaacaa atgcatcttt atgatcgctg
      661 aaatcaaccg catggccgcc agccagacgc ataaatgcca ttacccggcg ttctcctgct
      721 tgcagatatt ctagtaataa aggctcaata gcacggttta ccagagcaat agtcgggtga
      781 tcgcgttcac cgtcatggac ccacacgaca ggcgcatctt tgcgctgatg attaagccgg
      841 gaagctaaaa catggggaat gtaaggcgta tcgcacggac aaaacaaaaa ccactcacca
      901 gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc cgccagtgaa
      961 tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt ggcattaacc
     1021 acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa tggtttgccg
     1081 tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc gcctgccagc
     1141 acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat tgtgggattg
     1201 accctgctaa cgtgtctgtc tcaagagtaa ggagcatcac tatgaaatgt aaacgtctga
     1261 atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt aacctgttgc
     1321 aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg gatttgacgg
     1381 atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg gtgatcccgg
     1441 gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct cgcggcgtaa
     1501 ttaaagagta aaagcttgta agcggcgcca ccaaaatcat cgtgaaatga tatccttcat
     1561 cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt ccagacacta
     1621 cacccggata ccatcatgga cgctctgttt gagcaaggga tccgggtgga ttccggtctt
     1681 accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga gcgtcgacgt
     1741 tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct cgaagaacat
     1801 caatttgcgt tgcagctggt aaatgatgaa gttccggtcg cagcacctgt ggcctttaac
     1861 ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag cgtcggtggt
     1921 cgccagttcg aagctgataa tatcgatcag acggaagcgg ttgggcgtta tttagggcgt
     1981 atgcaccaga cggggcacaa acagcttttt atccatcgcc cgaccatcgg tctgaatgaa
     2041 tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc cgggttgaaa
     2101 gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca ctggcgggaa
     2161 gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct ctggcgcgat
     2221 ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca ggatttgtgg
     2281 atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat tattgaagct
     2341 tatgaagaat ttggcgagtt cgacaccgct gaaatcggac tgattgaacc tttacgcgcc
     2401 atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc cgcgttcccg
     2461 aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc gacttttata
     2521 gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta ttaatcggag
     2581 agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc
     2641 atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac cggtagctgg
     2701 cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc agtttgaaga
     2761 agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga agatgactaa
     2821 ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg catgggctgt
     2881 ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag gcgtacagaa
     2941 aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg caggtattaa
     3001 aggtgaagag tacgacgcgg cgtggaacag cttcgtggtc aaatctctgg tcgctcagca
     3061 ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg ttaacggtaa
     3121 atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg ttcagcagta
     3181 tgctgataca gtgaaatatc tgtccgagaa aaaataattc aatgt
//
LOCUS       DAJVXP010000243         3217 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVXP010000243 DAJVXP010000000
VERSION     DAJVXP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.247"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..432
                     /locus_tag="OQS10_004096"
     CDS             <1..432
                     /locus_tag="OQS10_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS2294967.1"
                     /translation="FRHWKGTGTVGVMVATNDPERPAPDESVINAVRDHILPLAPVAG
                     SGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALMLRDGVPEGRMYLSRISEAIS
                     LSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPARSK"
     gene            432..1022
                     /locus_tag="OQS10_004097"
     CDS             432..1022
                     /locus_tag="OQS10_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS2294968.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1009..2049
                     /locus_tag="OQS10_004098"
     CDS             1009..2049
                     /locus_tag="OQS10_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2294969.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2052..2597
                     /locus_tag="OQS10_004099"
                     /pseudo
     CDS             2052..2597
                     /locus_tag="OQS10_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2621..>3217
                     /locus_tag="OQS10_004100"
     CDS             2621..>3217
                     /locus_tag="OQS10_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS2294970.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTK"
BASE COUNT          842 a          721 c          928 g          726 t
ORIGIN      
        1 ttcagacact ggaaaggcac tggaacggtt ggcgtgatgg tggcgacaaa cgatccggaa
       61 cgcccggccc cggatgaaag cgtgattaac gcagtcaggg accacatcct tcctctggca
      121 cctgttgccg gaagcggatt gtatgtattc ggtgccacag aaaaagtcat cccgatgacg
      181 attgcattat cgaaagacac accgcagatc aggactgcaa taaaagcaga gctgaatgca
      241 ctgatgttac gggatggtgt gccagagggg cgtatgtatc tgtccagaat cagtgaggcc
      301 atcagtttgt ctgctggtga agtggcccac cggttaatcg tcccttcatc ggatattgac
      361 ctgggggaga ccgagcttcc ggtgctcggc gagatcacct ggcaggctta tgacccggca
      421 aggagtaaat aatggattcg ttacaggatg attatacaaa gctgctgtac ggactgatgc
      481 cgccggggcc tgcatggtca gataccgacg gtgtacttga cggtctggca ccatcgcttg
      541 tgcgtgttca ccagcgggct gatgaactgg tgattgaaat cgatcccggt cagtccacag
      601 agctgattga acgttatgaa gaattgtatg gtttacctga ttcctgttcc cctgtcggaa
      661 cccagacatt acgccagcgt cagcaacgtc ttgaagcaaa agccaatgtg gctggtggca
      721 taaatgagca gttcttcctg gatcagcttg aggcgctggg atataccggc gtaacgatcg
      781 aacagttcca gcacttggat gcaagccccg atccggaatg gggagatcgc tggcgttatt
      841 tctggcgtgt gacgttgccg atggatgccg gtgctcagtg gcagacatgc acggacgcct
      901 gcaacacacc gatccggacg tggggggata cggttgcgga gtgcgtgatt aataaattat
      961 gtccgtcaca caccgtcgtt ttattttcct atccagatga ggatgaagat gcacaggatt
     1021 gatacgctga ctgcggtaaa agataagttt ggtccgggta agaacggatt tactgacgga
     1081 aatcttcgca caggacgtct tgctacctgg ctgaacagtg ccatgtggaa tgccattcag
     1141 gaggaaatct gtggtgtcat tgagaaggcc gggatagaac tgaataaaga agaacacgac
     1201 cagctatata aggctatatt attgctggtg ggcggtgcaa ttaacgaaga ggcattgctg
     1261 ataaaaaata acctttcgga tgtggaagac agggatgagg ctgttgaaaa cctcggatta
     1321 aaacccacgg tggataaggc aaaaaatgcc gttcagcgtg atggcgacac catgaccgga
     1381 gagctgaaaa tccgtggtgt taatgcgctg aggattttca acgaagcttt tggcctgatt
     1441 tttcgtcgtt cggaagagtg cctgcacctt attcccacca gtgaaggtca gggggaaaat
     1501 ggcgatattg gtcccctgcg cccgttcacc attaatctgc ggacgggtga aatatccatg
     1561 tcgcataaag tgtctgttgg cggtggttcg caggtcaatg gtgcgctggg tatcggcgtt
     1621 cagaacgccc tgggggggaa ttcaattgtt cttggtgata atgacaccgg attcaaacag
     1681 aatggtgatg gcctgctgga tgtttatgcc aatagcgtgc atgtgttgcg ttttcagagt
     1741 ggcagtatcc agagtaataa agctgtaaac gttacaggac gggtaacacc gtcagactac
     1801 ggaaactttg atgcccgtta ccagcagcga aatggtggcg tgcaggatgt gcggtatggt
     1861 tatgaaatgt attacacccc cggcagtaac accgtttcgt ggacatttcg ctcaccttcg
     1921 ggacacgggc tgtcagggat atcgatatcg gataccggcc gtaactcagc ggataacgtc
     1981 aacggcgtgt attaccgacc gctgcaaaaa ctgattaatg gcacctggta taacgtagcg
     2041 agtatttaac aatgttgcat ttaaaaaata ttacggcagg taatccgaaa accgcggaat
     2101 aatatcagct gacaaaacag tatgatgtca cctggctttt ttcggaagac ggcaaaaact
     2161 ggtatgaaga acagaagaac tttgccagtg acaccataaa aatggtttac accggagacg
     2221 ggcgcgtggt gtgggtcggt aaggatgtga caggcattga accccgtaac gccagtgtta
     2281 ttgaagttcc tgatattacc gccaaccgcc gcattaccgc gccgggttac tggttttacc
     2341 gcaatgatga atttgtcttt gactacagac tcaaagcgga agatgagcgt gatgcccttc
     2401 tggctcaggt cagtgcccgg acaggggaat gggaagaaga cctgctgctg gggctaatca
     2461 gcgacgaaga ccgggaaaaa ctgaaagcgt accgtattta cgcgaaatcg ctgcaggcga
     2521 tggatttcag caccatcact gataaatcct catataacgc cattgaatgg tccgtctctc
     2581 cggaaggttc ttcctgattt aatttatcgc gagaaaaact atgtctgtag tgatatcagg
     2641 tgcgctgact gatggcgcag gcatccccat gtccggatgc cagattattc tgaaatcccg
     2701 tgtaaacacc tcagaagtgg tgatgcgtac cagggctgat gtagtgaccg gaaataacgg
     2761 tgaatattcg tttgaggcac aggtcggaaa atattgcgtg tatctgaaac gggactggcg
     2821 cgacgagtac tgtgttggcg acattgctgt atacgacgac tcaaagcccg gcacgctgaa
     2881 tgactttctg attgctcctg atgagggcga cctgaaaccg gatgtcgtca aacgctttga
     2941 ggaaatggtg gcgcaggcgc agcagagcgc cggggccgca gccggaaacg cacagcagac
     3001 ggcgcaggat gtggcggcag ccgcaggtta tgcccgcgca gcagagcagg ccaaaaatga
     3061 cattgatgct gcgctgaccg gcactctgaa aacggctaac catctgtctg aaatcgcagc
     3121 agcaggcgaa aaggcacaac agaagtcccg ggataatctg gggctgaaaa gtgcggccac
     3181 gatggaagca cagagcgaca tttacgaccg gacaaaa
//
LOCUS       DAJVXP010000244         3200 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVXP010000244 DAJVXP010000000
VERSION     DAJVXP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.248"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..461
                     /locus_tag="OQS10_004101"
     CDS             <1..461
                     /locus_tag="OQS10_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS2294971.1"
                     /translation="IQHLGYSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTN
                     HAERDKHLRSADFLNTAKYPQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLI
                     GQGDDPWGGKRAGFEAEGKIKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(503..1555)
                     /locus_tag="OQS10_004102"
     CDS             complement(503..1555)
                     /locus_tag="OQS10_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS2294972.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            1780..2700
                     /gene="lpxL"
                     /locus_tag="OQS10_004103"
     CDS             1780..2700
                     /gene="lpxL"
                     /locus_tag="OQS10_004103"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS2294973.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            2872..3069
                     /locus_tag="OQS10_004104"
     CDS             2872..3069
                     /locus_tag="OQS10_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS2294974.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          820 a          808 c          776 g          796 t
ORIGIN      
        1 gcatccagca ccttggctat agctggttat acggcacctt taaagatttc gacggtactt
       61 ttacctttga cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca
      121 gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata
      181 ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac
      241 tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga
      301 aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag
      361 gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag
      421 tggatctgat tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat
      481 ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca
      541 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
      601 gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt
      661 atttttccgc gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt
      721 tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata
      781 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc
      841 cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt
      901 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
      961 acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct
     1021 cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat
     1081 agtggttacg catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg
     1141 ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg
     1201 caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca
     1261 acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa
     1321 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
     1381 acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct
     1441 taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcaa
     1501 ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
     1561 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
     1621 taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg
     1681 cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat
     1741 acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc
     1801 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
     1861 ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg
     1921 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
     1981 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
     2041 atgggactga tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg
     2101 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
     2161 ttagttggca tccattttct gacactggaa ctgggcgcgc ggcagtttgg tattcaggaa
     2221 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
     2281 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
     2341 gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca
     2401 agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg
     2461 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
     2521 aaagggtatc aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa
     2581 actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
     2641 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
     2701 atctcccctg ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat
     2761 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct
     2821 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
     2881 tgtggaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
     2941 tttcttaccg gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag
     3001 cttggcgtta ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagtataata
     3061 ccttcctaaa tacactacct gagagaagag atgaaaagag ctatcagcta catgaggttt
     3121 tcaactgaga agcagataaa aggggactct atacgaagac agtctaagct ggtcactgac
     3181 tggttatcaa agaaccctga
//
LOCUS       DAJVXP010000245         3173 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVXP010000245 DAJVXP010000000
VERSION     DAJVXP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.249"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(26..316)
                     /locus_tag="OQS10_004105"
     CDS             complement(26..316)
                     /locus_tag="OQS10_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS2294975.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="OQS10_004106"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="OQS10_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS2294976.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="OQS10_004107"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="OQS10_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS2294977.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="OQS10_004108"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="OQS10_004108"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..2795)
                     /locus_tag="OQS10_004109"
     CDS             complement(2172..2795)
                     /locus_tag="OQS10_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS2294978.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
     gene            complement(2746..>3173)
                     /locus_tag="OQS10_004110"
     CDS             complement(2746..>3173)
                     /locus_tag="OQS10_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2294979.1"
                     /translation="FYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVC
                     NRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASP
                     QFFITGCSEHSLPGSYSALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          827 a          853 c          726 g          767 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgaccatt ccattcaaac
     2461 gtgaacggct tcgcctcctg ttctgtgcgc catgcttcaa tttcctgacg tctggcctct
     2521 ctggccgctt ccagcatttc tggtgtcaca gtgaatgggg ctatctcacc ccatttgcca
     2581 ctttgcagtt cctgccagat tcgctgaccc gtcggtgcga catcatcagc ggtggctgtg
     2641 taggggactg cctggtccct gtcgtcaaaa aaaacgtcac agtctactgc gccactttcg
     2701 gtataacggg gattaatgat ttttttaatt tccacggtgc attcctcacg atgtgcgaat
     2761 aaaaagcccg ggcattgcgc cagagacatg agcatccggc accccggaca gggcgctata
     2821 tgaccccggt aatgaatgct ctgaacatcc cgtaatgaaa aattgtgggg atgctatata
     2881 cgttccggtg ggagtactgg gcactgaaat ccccaccggt cccagtcgtg agcctctgta
     2941 tgactgcccc ctgacaagtc tgatgacttt atcaccgtca gcttctccct ggtacgcagc
     3001 aataatcagc ccgccaatgt cagggtctcc ccatctgttg cagacagagc tcgccacgat
     3061 tctgtaaata atatcttctg tggttatatt tattttcacc cagccagtca gtctggatat
     3121 gggccagtag cagtagcggg tgtgataaat ggggccgtta atgccgtaaa aag
//
LOCUS       DAJVXP010000246         3043 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVXP010000246 DAJVXP010000000
VERSION     DAJVXP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.250"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..2991
                     /gene="lacZ"
                     /locus_tag="OQS10_004111"
     CDS             <1..2991
                     /gene="lacZ"
                     /locus_tag="OQS10_004111"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCS2294980.1"
                     /translation="AAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE
                     SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPAENPTGCYSLTFNI
                     DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGKNRLAVM
                     VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPSTQISDFHVATHFNDDFSRAVLEAEVQ
                     MYGELRDELRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPALWSA
                     EIPNLYRAVVELHTDDGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP
                     LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRFGLYVVDEANIETHG
                     MVPMNRLTDDPHWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS
                     VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL
                     CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG
                     DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL
                     LHWSVALDGKPLASGEMPLDVAPQDKQLIELPELPQPESAGQLWLTVHVVQPNATAWS
                     EAGHISAWQQWRLAENLSVTLPAAPHAIPQLTTSETDFCIELGNKRWQFNRQSGFLSQ
                     MWIGAEKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC
                     TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG
                     LTCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT
                     RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW
                     SPSVSAEFQLSAGSYHYQLVWCQK"
BASE COUNT          680 a          837 c          872 g          654 t
ORIGIN      
        1 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
       61 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
      121 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
      181 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
      241 acggtcaatc cgccttttgt tcccgcggag aatccgacgg gttgttactc gctcacattt
      301 aatattgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggtgttaac
      361 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagccgtttg
      421 ccgtctgaat ttgacctgag cgcattttta cgcgccggaa aaaaccgcct cgcggtgatg
      481 gtgctgcgct ggagtgacgg tagttatctg gaagatcagg atatgtggcg aatgagcggc
      541 attttccgtg acgtctcgtt gctgcacaaa ccgagcacac aaatcagcga tttccatgtt
      601 gccactcact ttaatgatga tttcagccgc gcggtactgg aggcagaagt tcagatgtac
      661 ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt ggcagggtga aacgcaggtc
      721 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
      781 cgcgtcacgc tacgtctgaa cgtcgaaaac ccggcgctgt ggagcgccga aatcccgaat
      841 ctctatcgtg cggtggttga actgcacacc gacgacggca cgctgattga agcagaagcc
      901 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
      961 ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     1021 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
     1081 cgttgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg cttcggcttg
     1141 tacgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
     1201 gatgatccgc actggttacc cgcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
     1261 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg agtcaggcca cggcgctaat
     1321 cacgacgcac tctatcgctg gatcaaatct gtcgatccat cccgcccggt gcagtatgaa
     1381 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
     1441 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
     1501 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
     1561 ggcttcgcta aatactggca ggcgtttcgt cagtaccccc gtttacaggg cggcttcgtc
     1621 tgggactggg tggatcagtc gctgattaaa tatgatgaaa atggcaatcc gtggtcggct
     1681 tacggcggtg attttggcga tacaccgaac gatcgccagt tctgcatgaa cggtctggtc
     1741 tttgccgacc gcacgccgca tccagcgctg acagaagcaa aacaccagca gcagtttttc
     1801 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     1861 gataacgagc ttctgcactg gtcggtggcg ttggatggca agccgctggc aagcggtgaa
     1921 atgcctctgg atgttgctcc acaagataaa cagttgattg aactgcctga actaccgcag
     1981 ccggagagcg ccggacaact ctggcttacg gtacacgtag tgcaaccgaa cgcgaccgca
     2041 tggtcagaag ccggacacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagc
     2101 gtgacactcc ccgccgcgcc ccacgccatc ccgcaactga ccaccagcga aacggatttt
     2161 tgcatcgagc tgggtaataa acgttggcaa tttaaccgcc agtcaggctt tctttcacag
     2221 atgtggattg gcgctgaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
     2281 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgacccgaa cgcctgggtc
     2341 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
     2401 gatacacttg ccgacgcggt gctgattacg accgctcacg cgtggcagca tcagggaaaa
     2461 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
     2521 gttgatgttg aagtagcgag cgatacaccg catccggctc ggattggcct gacctgccag
     2581 ctggcgcagg tagcagaacg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
     2641 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
     2701 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     2761 ccacaccagt ggcgcggcga cttccagttc aatatcagtc gctacagcca acaacaactg
     2821 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     2881 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtttc agcggaattc
     2941 cagcttagcg ccggtagcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
     3001 ggcaggccat gtctgcccgt atttcgcgta aggaaatcca tta
//
LOCUS       DAJVXP010000247         2994 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVXP010000247 DAJVXP010000000
VERSION     DAJVXP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.251"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            18..1076
                     /locus_tag="OQS10_004112"
     CDS             18..1076
                     /locus_tag="OQS10_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCS2294981.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OQS10_004113"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OQS10_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS2294982.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="OQS10_004114"
     CDS             2805..>2994
                     /locus_tag="OQS10_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCS2294983.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAJVXP010000248         2992 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVXP010000248 DAJVXP010000000
VERSION     DAJVXP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.252"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..200)
                     /locus_tag="OQS10_004115"
     CDS             complement(<1..200)
                     /locus_tag="OQS10_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS2294984.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQA"
     gene            complement(203..481)
                     /locus_tag="OQS10_004116"
     CDS             complement(203..481)
                     /locus_tag="OQS10_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294985.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(482..1039)
                     /locus_tag="OQS10_004117"
     CDS             complement(482..1039)
                     /locus_tag="OQS10_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294986.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(2603..>2992)
                     /locus_tag="OQS10_004118"
     CDS             complement(2603..>2992)
                     /locus_tag="OQS10_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2294987.1"
                     /translation="LAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLRE
                     GFDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQT
                     LISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          832 a          625 c          558 g          977 t
ORIGIN      
        1 gcctgaactt catgatgcaa aaaccagatg acatacttca gataataatc acgagtgcca
       61 taacgaatca ttggattacc gtaagcatca tctcttgtct taacctctcc accatcaatc
      121 cattcaagca ttcctgtctc ataattaatc atgaagtaag ttgcaaagaa ccgtttaata
      181 tcatctcgcc attgcttcat aatcagacct cctgagacag cttgtgaatt ttgatactta
      241 gtaaccttgc taaatccagt ccaaaagcat ttaaggattt cagactatca aggtcatctt
      301 gcttaatcct gatatggtgt cttgcagcct ctaagcgact attaagcaaa tcctgttcgt
      361 taatatcacg gttaattgca agcttcatca ctgctgagtg tggcagacct gtaatgtttt
      421 tgattcggtt cagttcatca aaggctgtct ttgtaactct ggttaatgtg tgtttattca
      481 tttaaaaatc ctctgtaact aaataggggt caaactcgaa atctggttgc tcgttttcca
      541 ttggctcaat attcccaacg gcaatatgat gttcaacaat gccaattaat ttcgcgcaca
      601 tcacagcaag caaagcgcaa tatttatcgc ttggtaattc gtaactaagt ctggttgcct
      661 gtcgctgaag atgatttgtc acggtggatg cgttaatttc gtcaagattc tttagcagat
      721 aaaatagttt tttgttgtat ttcgggtggc cttcacttct acagaaccca ataaaaacat
      781 catcaagctt gcttggtaac tgctcgtaac tgtcaaagtt agccagataa tttttaatag
      841 tattctttgt tgttttgcgt actggcataa atctgtctcg tggcgtaatg ctacgaatga
      901 ccgcttcaat gttcttacgt tcggatttgc taaaggttgg gacttcgtag ctggtggggt
      961 tgtttgtgta agcgtaggag ttgtgcttgt tgcgttctct tgatgcctga agcatcgctc
     1021 taaattctga atactgcatt catgttctga ctctcataag atgttattat tctgttaacc
     1081 tatatacaat attagcattt tcaaagacta atgtcaagta tttttaacat tttcgtaaca
     1141 tctgtggtat aatgggttta tggggagagt aaaatatgag gcgtggctat gtatttggtc
     1201 gcatattttg cattgcacac agacctcttc aatcgccctg taacgagtta aacgacctct
     1261 tcaggtcaac tcataaggtc aggtactttt tgtgtcagaa tgcgattgag agcgtcttac
     1321 aaggaaaact aatggtaaat ctccctcctg agctggggtc acctgatgta tggactggtg
     1381 gaggtattgg gctgattaca ggcctgctac ttcgctacgt cctcgtcaga gtgcttgttg
     1441 tctttaaact gttcggtcgt acatggacgc ttgaccttaa ggataacgac cacacagacc
     1501 cccgttaaac tccttcaaaa cccctgcacg agatgacaga tagaagtcta tggtacaggg
     1561 gtgtctttag cattttgtgc tatctattaa aaaaatagct tgacagaacc ggaaagccgt
     1621 atctatcaag cattcacctc tctgacagta atttctaaaa gttcttattt ttcaataagt
     1681 tgtaattagc catatataga gaactaaaag attttttagc tattttgttt aaaagcatat
     1741 caaacgatac attttattga tttaggtcaa agacaaaagc attgacaaaa tggtaagatt
     1801 gttcacatga ccttacttaa cagactttta agagactttg aaacagcctg tttaacaggt
     1861 cactacctct ggtcttatcg ttcccttctt tctaggtggt tatctgttaa gagtttttca
     1921 gtttttattc ttaagataat tgttaagatg atactctccc ttcgggttat atcgtatctc
     1981 ttctcccgtt aaatatccct tcacagtcag actaaataac acctgtgtag ttcttggtag
     2041 aatctttcaa ggtaacttag ctctgagttt atgaagttga cctatgagat agactctacc
     2101 gaatctgtta gtcaattgtt gcttaggtgc tgctaaggct tacttagtgg aaagagaggg
     2161 gatatgtgac cataggggaa cccctcctgt taaaagacta cttagaagtc tgaggtttta
     2221 gtaaaatctg tgaatatggc ttctcagacg ctctgtaacg cattctaagc gattttaccc
     2281 ctttatttta aggtggttag tacatcccta taggttaccc ctaaaaatgg ggttaaaact
     2341 cgctacagag ctttctatga gattagcttc taatcgaatc aactatgagg tttggtagga
     2401 ttgtgcaaaa tctgtacagt aacttttcag aatctgaaaa tttttataaa aaatgactag
     2461 ccctctttga atctcatctt ccccgaatct tcaaaaatat ctgacagatg tctgtagctg
     2521 ctaagtaccc gtcacaccag ctatgtgacc cccttggcaa aaaagccaaa tatcagctac
     2581 atccatgtaa ctattcagta acttatttag caagtctgta ataagttgct ctagagattc
     2641 ctaactcttc ctgaatctgc aacctcgtct taccttgtga aataagcgtc tgtacagcct
     2701 ctgaagaagc ttttacaggc ctccccatat gtaccccttc agctttagca cgttcaatac
     2761 cagcctttct acgctctgct atcaagcttc tttcaagctg tgccaacgtt gacataatgc
     2821 ctaacattgc ttcacccata gcgcttgata agtcaaaccc ttctctaagg ctgataactg
     2881 tcacaccttt agcctgtagc gctttaaccg ttgataacac atcaattgtg tcacgtccaa
     2941 gacggtctac agctaccaca actacggtgt caccttctcg tgcataagct aa
//
LOCUS       DAJVXP010000249         2958 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVXP010000249 DAJVXP010000000
VERSION     DAJVXP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.253"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..239)
                     /locus_tag="OQS10_004119"
     CDS             complement(<1..239)
                     /locus_tag="OQS10_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2294988.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEG"
     gene            complement(300..902)
                     /locus_tag="OQS10_004120"
     CDS             complement(300..902)
                     /locus_tag="OQS10_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS2294989.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(839..1582)
                     /locus_tag="OQS10_004121"
     CDS             complement(839..1582)
                     /locus_tag="OQS10_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2294990.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(1587..2285)
                     /locus_tag="OQS10_004122"
     CDS             complement(1587..2285)
                     /locus_tag="OQS10_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS2294991.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(2295..2624)
                     /locus_tag="OQS10_004123"
     CDS             complement(2295..2624)
                     /locus_tag="OQS10_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2294992.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(2624..>2958)
                     /locus_tag="OQS10_004124"
     CDS             complement(2624..>2958)
                     /locus_tag="OQS10_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS2294993.1"
                     /translation="DLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKL
                     GGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDG
                     GRLSGAYR"
BASE COUNT          650 a          994 c          679 g          635 t
ORIGIN      
        1 ccctccggcg gtgtctgctc ctgctcacct gcacggaaca ccaccgtaac accggaaaag
       61 tttgtcttcc cctcgctgtc caggaccggc gtaccgttca gcagcacgct ttttaatcca
      121 tccaccggac cgtcaaccgg cccttcgctg atggcatcaa tcacactcag caactgcgtg
      181 gatttcaggt tgtccttcgc ttcgcgcggg gtatgcccct tactgctgcc tttacccatt
      241 cctcacgctc cataaacgac aaaaccgccc gcaggcggtt tcacataaaa cattttgcat
      301 cagcgaccaa tcaccacaac ctgaccacca tccccttcgt ctgccgtgct gatctcctga
      361 gaaaccacac gtgaccccac gcgcatttca ccgtacagaa cgggcagaac attgccctgg
      421 gcaaccatgt tatccagtga cgagaaatac gtgttctgct taccgttatc cgttgtctgt
      481 gtacggggag ttctggcttt cggtgccagc atctgtgcaa caccgccaag cgtcatactg
      541 gcaccgagag aaaacagcag attactcgcc ataattccta cccccggcat ccatatagca
      601 accgccataa cagccgcccc cagcaccgcc tgaaacacac cgccactttt agctcccacc
      661 agacgcggta ctatgtggat cactgcacca tttgccagcg gctcattaag acgggcagac
      721 aattcggttt cacctgcatc acgcccggca atgcgcacct gataccagcc gtcactcagt
      781 ttctgacgaa acgccgggag ctgtgtggcc agtgcccgga tggcttcagc ccccgttttc
      841 acacgaaggt cgatgcggcg accaaatcgt tgtaaatccc cgtaaaggca gatgcgcgcc
      901 atgcccggtg acgccagagg gagtgtgtgc gtcgctgcca tttgtcggtg tacctctctc
      961 gtttgctcag ttgttcagga atatggtgca gcagcccgcc gtcaccgcag taaatggcgg
     1021 catgattcgg caccgatgaa ccaaaacagc acagcagcac atcgcccggc tgtgcctctg
     1081 tcagtgcgac acggtaaaaa cccgtcacct ccatattgtc aagatagaga ttctgaccgt
     1141 tacgccacca gtcatccccg cgatgaaaat ccggcatctc aatccctgcc agatgataag
     1201 catcccggaa cagcgtgtaa cagtccgtca ccccgtgctc aaagcgccgc ccggtgagat
     1261 gcggcacaca gcggaacttg tgaatcgccc cccggcagac cagccaccac ggcaaatcac
     1321 tctgcacctg cagccgccgg tcagcctcac tcagccaggg cagaccaccg ggatgactgt
     1381 ggaccagcgc cacaatctca ccctgcattt gtgcccgcag ccagtcctcc ggagccatcc
     1441 ggaaccgttc ctccggctca ccggagatat tctcgctggg aagatacctg tctccctccg
     1501 gcgttcttac cacgaagcca cacgactccg ctggcgcaca tcgccgggcg tgcgccagaa
     1561 tcgctgattc tgtctgtgtc atggatttac tgagaaagtt tattgatgga aaggaaaccg
     1621 ccgaaattac cggtattgtt gcgcaactca cacccgcgca tacacttgct gcatttgtcc
     1681 ttccggatat ccgtcgttgg tttgtcgaac tcgtcagcca ctgccctgcc cgtgtaacca
     1741 cactcatcag aacggtaagt ccacatacag gtattcgcca gcatgatacg ccccgggaaa
     1801 accgcgccat ccgtctccgt tggtgtggcc agcacaaaag aggcactgac cgcgctcagt
     1861 tcgctgcact gctcgatgcg ccagcggctg atcacctcct gttccggatc ggcgtcgctg
     1921 tttccgttga cgaagttcac cgcatccaga aaacgggcgt aaaccttacg cctgaccacc
     1981 gttccgccga ccagactctg caggtcttcc gccatcccgg tgaccattcc gtgcaggtta
     2041 gaaaccgtca gtgtcggacg ggcagcactg cccttgccgt tcagttcaaa tcccgtcccc
     2101 tgaatggggt atgcctgata ctgccgcccc tgccaggtaa ccggctcacc tttttcgttc
     2161 tgctcattac agaaaaaata acgttcacca ccgacctctg tcagatcgat ttcccagagc
     2221 accacctggg ctgactgagt gaggcgtgtc gtctcatgat gtgtttcctg tggaatatcc
     2281 tgcatcagag cctcctatgc cacgacctgt tcaaaatctg ccgttatggt tacccacaac
     2341 gcccccacgc ttgccgacca tttacgacaa accaccctga tcggcttcca gtcataaggt
     2401 ggcgtccact gaaatgcgcg gacgccaccg tgccgttcca gaaaggcttt taaaaatggg
     2461 tgttcacctt tacgaacacg tatcttcacg ctgtaagtcg acaactggtt attcagtccc
     2521 gccgcacgac gctgttcata accatcgccc agcttcactg tcaccacttt cggctctgat
     2581 accacattca tatccgggcg cactttccag tgaaacgtct ccattaccgg tatgctccac
     2641 ttaaccggcc accatcacgg gcctgctgtt gcataaagtc cgcggccgct tttttcccga
     2701 ggtcataaac caccttcagg gcagccggac ctatctgccc gttcgtgcca tcgttattga
     2761 tctcaatgtt gtactgcggg gcaaacatca ccatgcctga accaccagta tccgccacac
     2821 cccccagctt accatcagca ccgcgacgca gtggcagaat ggcttcaggt cccgcttccc
     2881 ccatcacacc cgcgcctttt gcaaaagcaa aaaacgtcgg acggttaacc accgtgccac
     2941 tgtagcgact caaatcag
//
LOCUS       DAJVXP010000250         2943 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVXP010000250 DAJVXP010000000
VERSION     DAJVXP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.254"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1735)
                     /locus_tag="OQS10_004125"
     CDS             complement(<1..1735)
                     /locus_tag="OQS10_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS2294994.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRG"
     gene            complement(1820..2155)
                     /locus_tag="OQS10_004126"
     CDS             complement(1820..2155)
                     /locus_tag="OQS10_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS2294995.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2155..2511)
                     /locus_tag="OQS10_004127"
     CDS             complement(2155..2511)
                     /locus_tag="OQS10_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS2294996.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2511..>2943)
                     /locus_tag="OQS10_004128"
     CDS             complement(2511..>2943)
                     /locus_tag="OQS10_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2294997.1"
                     /translation="EGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAYVLRKLKS
                     VITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYDLFKQYLI
                     VERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          658 a          887 c          636 g          762 t
ORIGIN      
        1 caccacgtgg catctgccat ccggtcatgt catagacaaa atcatggaac tgtttgcccc
       61 actcgtcgaa cttatcattc agaccaaatc cactgttcag tgcatcagcc acaaaatttt
      121 taacaagcca gggatgtgct ttttcaaact cctgcgccat catgcccagt tcaagcaatc
      181 caccaatcag actgatcttt ccgaggccct ttaatttcag ccctgttttt cctgccgccg
      241 ttgtccattg attctgggcg actgttgccg cacgaatact tgccaccgcc ttcacaccaa
      301 tatacagggc agaaaccgtt gctattgttt ttactgcctg ttcccatccc cccattgctc
      361 tgacaacctg atcaacctcc tgccagactt ttttcacaac cggaccgacc gtttcccagt
      421 tatcaattat gagatatgca ccaccgacga gaagcgcaat taatcccttt gccggcgtca
      481 tattcatcac gctacccatg atttttgtta tgcgtgtcaa tgtgccaatc gccacgccca
      541 tcgtcagtaa tgccgcacca gttttcgcga tggtttttac gacctcagga ttttctctga
      601 caaacgtgcg cacttcttcc agaaaaggct tcatctcttt tatgccttca ttgagtgaag
      661 gcaggaaggt ttcccccagc gtggaagaaa tcgcattgat ctggttctgc agcagcaaca
      721 actgattttc tgtcgtcgcg gcacgggcgg catattcttt ctgcattgag ccgccatact
      781 gctgcgcatc cgccacacga ttaaagttgg ttcgcaacaa atccagattc gtgagcagcg
      841 gcgcaatggc ccccagggat tccttcccga acagcgcttt cagcacagca gcctgttttt
      901 ctttcggcac ttttgccaga gaatccagta cgtgcagcat ggccccgcgg gcgtctttct
      961 gcatatccgc cgccagtttt ttcgggctaa tgcgcaacga gcggagcact tttttctgcg
     1021 atttggtggc agaatccctc gctgtcagcg acaacataaa attctttatc cccgtcgcag
     1081 caatttctga ctccaccccc attccggcaa tggttgcccc cattgcggca atctctccgg
     1141 aggccacacc cgcaacactg cctaaagggc caatacgggt cacaacatca gaaatctttt
     1201 tcgcacttgc aggaccggta ttaccaagat agttaatctt atccgcaagc cctgccacct
     1261 ctccctgtgt cagtttaaag gcagtgcgcc actgcgccat catctgaccg gattcttctg
     1321 ccgtggtgtc aaaggccacg cccatcttca cggcatcgtc agtaaactgc atcagctcgt
     1381 cacgggcgat accagcctga ccacccgccg ccacaatttc ggcgatacct tccgccgaca
     1441 tgggcagttc tgttgacagg tcgcgcacct gctccgtcat tgccttaaac gcttccggcg
     1501 tatccagacc atccaccact ttccggacat ccgccatttt tgattcaagg gcaatggcag
     1561 atttgaccgg gagcgccagc gcccccagta ctgcggttcc ggcacccgct gcgcccagta
     1621 acaggctggc aaactccttt ttaaaacctt tcagctggcg ctgcattccc ttcagcggtg
     1681 ccgatacctt atccacggca gtgatgatcg ccttcagctg aaaactgtca gccatgcttc
     1741 atctcctcgt taatgcggac ggcctcggct tccagttctg taaactgcga aacagccccc
     1801 cgtcgcagct ccagtggatt cagtttccag aaccaggcaa cattgtagag tcgtttccgg
     1861 aggtgcttcc cgtctccgac tgggtaaaaa aacgcaggat ctgcatgctg gtcttaaaaa
     1921 tatccagttt tgccatctgc gccgccgatg aacgcgggat ccccgccagc aacgggatat
     1981 atttcagtgc cacctggctg tccagtttaa tactgccttc accggaaata atgaaaggaa
     2041 aacccagcgc ctcgatttcg tcatacgtgg gctcgcgcaa ctccagcaca tgcagcattt
     2101 cgttatgcgc tgtcaccggt ttttttaaaa ctatttctgt aacgttcatt gccattactg
     2161 ataccctccc tcttcgccgt ggaattcaag atctgccgtg ccttcttcgg cattatgatt
     2221 agcctctccg tgcagccatg ccgccgaaag cacatacacc atgccgtttg ccagctcagc
     2281 ggtaatcgtc atctggtctg aggtggtaat tttgtcgacc ggaaagtttt tgggcacttt
     2341 aaacgtgccc ttaacgtaag gtgaccgcca ggtctccttg taatccacat cccctgccat
     2401 gccgacgatg tcatcccgga cattggtgtt catcggcact tcaatgccac ctgtcagcga
     2461 taactgctga ccatccactt taaaaaaaca ggttccggcg atctttgcca ttatgctgac
     2521 tcctctgaat actgaagacg gaactggtta accaccgcaa agacacgtaa ctgattaaca
     2581 taatccggcg ggaacagcgt attcagtcgg ttcgggttat ctgcatcacg ctcaactatc
     2641 agatactgtt taaacagatc gtaattttcc acaataccgg cacgctccat ctgacgatat
     2701 gtcgccagaa gttccccttt gataacggca ggagtgacaa tcgcctgccc cggaccaaaa
     2761 cgggtaccat catttgccag cttgtgacgt ccgtacttgc ttgtgatgac cgatttcagt
     2821 ttgcgaagaa catatgcact ggtatgcaga gtttcactgt ccagatagct gttgtctgcc
     2881 acgccatacg cattcttttt gtaggtggtt acggaacgct ggatctgcaa cgtgccgcct
     2941 tcc
//
LOCUS       DAJVXP010000251         2924 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVXP010000251 DAJVXP010000000
VERSION     DAJVXP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.255"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(289..882)
                     /gene="tehB"
                     /locus_tag="OQS10_004129"
     CDS             complement(289..882)
                     /gene="tehB"
                     /locus_tag="OQS10_004129"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS2294998.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(879..1871)
                     /gene="tehA"
                     /locus_tag="OQS10_004130"
     CDS             complement(879..1871)
                     /gene="tehA"
                     /locus_tag="OQS10_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS2294999.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1995..>2924
                     /locus_tag="OQS10_004131"
     CDS             1995..>2924
                     /locus_tag="OQS10_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295000.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKV"
BASE COUNT          818 a          698 c          686 g          722 t
ORIGIN      
        1 gatgattata ttacttttta agttatagca aaaagcgccg cttcgtgttg tggcaaaaaa
       61 agtgttggtg aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa
      121 ggggctaagt caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaaacttcga
      181 ctactgtgat taatttcaca tcccttcgat aaaaaatatg cttaccgaaa agaagaacag
      241 taaatcatct cagcgtgggg aattttcacg cgccttattc ttagcgggtc attttttacg
      301 tgccagcatc gtggcgaaac gcagtttaat acgattaccg ttggcgtcgg tgcggtgcag
      361 ctcgccgacg tcttcattgt atttcaccct ctcccagcct tcgtaatatc gacgtaattc
      421 tccctctttg aaggcaaacg ggaagccgac ggtacatgga taatcagcgg tatccatcgc
      481 cgccacaatc aggttgtaac caccaggttt agtgcaacgt tgcatattgg caatcagccc
      541 tgggatggtt ttagcctcaa ggaacatcag taccacagtc gaaagaataa aatcgtactg
      601 tctatcaaat gtgaggttat tcagatcaac gactcgggtg tgtaaattat ccagattttc
      661 aatggattta atgcgctcta cattggcgat actcatggca tttttatccc atgcgtcaac
      721 atcataacca ttggctgcca ggtaaagact gttacgacca ttgccacagc ccagatccag
      781 cgttttaccc ggtttaacca ctttcaccgc ttccagtact tcagagtgtg tgcgggttaa
      841 ttcatattta tcagtaaaat agttttcgtc acgaatgatc attctttgtc ctctgctttc
      901 attaaaacgg cgcgctcggt tctgactaac aattttccct gcatcagaag cgcaaaagta
      961 cggatgagca gtattgcaat aataaaattg gtaaagataa acagcggtac cgccagcgta
     1021 tggaaaaatc cattttcgct gccactcccc agatgcaaac cggtggttgc cagtgcagat
     1081 acgccgaacg agaaactcca gaatgaagca ttaaatggct gggagagata ccatggcatc
     1141 agacgtagca taaacagcag ttgcagcagt ccataaccaa aaagcatttt cgccagcgta
     1201 tcaccctcgc cgccgttgac gctcaaccag gcactacagg ccaccagcgc aggagcgagc
     1261 tgaatgccga gtgatgtccg cagtgccgtg ggtaattctc ccgaactgcg cagacgctgc
     1321 aagatcaccg gttcaagact tagccatgag aaaacgcctg cgcctaaaaa caccagaccg
     1381 gcgtcggtgt agcccaacgc accacaggcc attgcgctga taaagttgtt ggcaactgtc
     1441 ggcagataca gtccaggcgt ggtagcttct tcagggtgag atccgcgcca taatcccgca
     1501 gtttgccagg cggcataagc caactgaacc acgacaccaa aactgaacag gcacaccgcc
     1561 agtgggcgaa accacggaac aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa
     1621 ctcacaaaac tgctcagcac tggatggcga acttccgcca gcacgctatg cggaaagcgt
     1681 atgagtcggg taataaatgc gctagttaat aatccccaga tgatcatcgc cagaatcacc
     1741 agcccatccc ctaaccagtg gctgacctgc caaacctggc tggcatagcg ccaggcaaat
     1801 cccatcccta tcgtccccaa cacaatacca aagtagcctg ccggcaaatt gagcacttta
     1861 tcgctctgca tcttcttact cattttgttt aatttataaa gtatatttga aatgcatttt
     1921 atggaatttt ctcgagcata gccagagccg cagaatttgc tacggttata gtaagtcacc
     1981 tgtgagaaaa cgctatgcgc aaatatcgtc taagtgaaga acagcgagcc tttagttatc
     2041 aggaagatgg cactaaaaaa agtgtgttat tacggcagat tatcgccatt agcgatttta
     2101 acgatgtgat agcaggaacc gctggcggct ggatcgatcg cgaaacggtg ttagcacagg
     2161 aaggaaattg ctggatttac gaccagaatg ctattgcgtt cggcggaacg gtgatttctg
     2221 gtaatacgcg tatcaccggc accagcgtgc tgtggggaga ggtttacgca acggataacg
     2281 tctggatcga taatagtgaa atcagccagg gtgcatatat aagtgacagt gtcaccattc
     2341 gtgactcatt agtttacggg caatgtagaa tatttggtca cgccttaatt gatcaacatt
     2401 ctatgatcgt cgccgcacaa ggcttaacgc ccgaccatca actcctgctc caaatttatg
     2461 atcgcgcaag ggttagcgcc tcgcgtattg tccaccaggc acaaatttat ggcgatgccg
     2521 tcgtccggta tgcttttatt gaacatcgcg ccgaagtttt tgattttgcc agtgttgaag
     2581 gtaatgaaga gaacaacgtc tggctatgcg attgcgcaaa agtctacggt cacgcgcagg
     2641 tgaaagctgg catagaagaa gatgccattc cgacgattca ttacagttcg caggtggcgg
     2701 aatatgccat tgtggaaggt aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg
     2761 ccgtggtacg tggtggaccg attctgctcg acgagcacgt tgtaatacaa ggtgagagcc
     2821 gtatttcagg tgcggtgata attgaaaacc atgtggagtt aaccgaccac gccgtcgttg
     2881 aagcctttga tggcgacacg gttcatgtcc gtggtccaaa agtc
//
LOCUS       DAJVXP010000252         2797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVXP010000252 DAJVXP010000000
VERSION     DAJVXP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.256"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..617
                     /locus_tag="OQS10_004132"
                     /pseudo
     CDS             1..617
                     /locus_tag="OQS10_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            614..1093
                     /locus_tag="OQS10_004133"
     CDS             614..1093
                     /locus_tag="OQS10_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCS2295001.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="OQS10_004134"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="OQS10_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS2295002.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2467..>2797
                     /locus_tag="OQS10_004135"
     CDS             2467..>2797
                     /locus_tag="OQS10_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295003.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          620 c          590 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttccctta tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAJVXP010000253         2781 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVXP010000253 DAJVXP010000000
VERSION     DAJVXP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.257"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1258)
                     /locus_tag="OQS10_004136"
     CDS             complement(<1..1258)
                     /locus_tag="OQS10_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCS2295004.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="OQS10_004137"
     CDS             complement(1415..1624)
                     /locus_tag="OQS10_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295005.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="OQS10_004138"
     CDS             complement(1647..2357)
                     /locus_tag="OQS10_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295006.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
BASE COUNT          808 a          583 c          459 g          931 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcca
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt c
//
LOCUS       DAJVXP010000254         2742 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVXP010000254 DAJVXP010000000
VERSION     DAJVXP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.258"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..917
                     /locus_tag="OQS10_004139"
                     /pseudo
     CDS             1..917
                     /locus_tag="OQS10_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            933..1682
                     /locus_tag="OQS10_004140"
     CDS             933..1682
                     /locus_tag="OQS10_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS2295007.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1684..>2742
                     /locus_tag="OQS10_004141"
     CDS             1684..>2742
                     /locus_tag="OQS10_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295008.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          862 a          555 c          570 g          755 t
ORIGIN      
        1 tcacttagcc gcgattttga tatcgggcca ttaaaaaacg tctccgcttc tctaacattt
       61 agccgaataa actgggaaga agacaaccag gatcaactgt acctaaatat ttcgattccc
      121 tggggaacca gtagaacatt gagctatggt atgcaacgaa atcaggataa caagatttcg
      181 catactgctt cgtggtatga ctcttccgat cgaaataatt cctggagcgt ttctgcttca
      241 ggcgacaatg atgaattcaa agatatgaaa gcgtcactac gcgccagtta tcagcataat
      301 accgagaacg gtcgtctcta tctctccggg acatcacagc gagacagtta ttattctctg
      361 aatgccagtt ggaatggttc attcacggcg actcgtcacg gagccgcttt ccatgactat
      421 agtggtagtg ctgactcgcg ttttatgatc gacgcagatg gtgcagaaga tattccgttg
      481 aacaataaac gcgcggtaac taatcggtat ggcatcggag ttattccatc agtcagcagt
      541 tacataacaa catcattaag cgttgatacc cgaaatcttc cagaaaatgt ggatatcgaa
      601 aactcggtta tcaccaccac cttaaccgaa ggtgctattg gctacgccaa acttgatacc
      661 ccaagggcta tcaaatcatg ggggttattc gcctggcaga tggtagtcac ccaccactgg
      721 ggattagcgt aaaagataaa accagccata aagaattagg actggttgct gatggcggct
      781 ttgtatacct caacggcatt caggatgaca gcaaacttac tttacgctgg ggtgataaat
      841 cctgttttat tcaaccaccc aatagcagca acttaaccac cggaacggtt attttaccgt
      901 gtattagcca aaattaattc aggatgaaac taatgcgcta cttaaacaca aagaatctca
      961 ttgcagcagg agtattactc gcctgcatga gcagcattgc ctggggtgcc atcatcccgg
     1021 accgtacccg tatcataatg aatgagtcag ataaagggga agcattaaaa cttactaatc
     1081 aaagcaaaaa tctaccctat ctggcgcaaa catggataga agatacgaaa ggcaataaat
     1141 cacgtgactt tattgtcacg gttcccccta tggtacgttt aaatcccagt gagcaaattc
     1201 aaataagaat gataagtcag gaaaaaattg ctcaactgcc taatgacaga gaaacgctct
     1261 tctattttaa cgtgcgagaa ataccgccaa aaacagataa aaaaaatgtg atgcaagtca
     1321 ctatgcagca cgcattgaaa ctattctggc gaccaaaagc tattgaatta gaagatgatg
     1381 gggtaatgtc ttacgaaaaa gttgaaatca tccgtagaaa tgatggttcg atccgcttta
     1441 acaataagat gccttatcac gttacgctgg gttatatcgg aacaaacgga atcacaatgt
     1501 tgccacaaac gcaaagttta atggtaaccc cttatagcca tgcagatacg cagtttaaaa
     1561 atgttccgtc agcttttcag gttggttata tcaatgattt tggtggatta agtttttatg
     1621 aaataaactg cccgacggtt aataactctt gtaatgtttc cgtagccaaa cgagataaat
     1681 aatatgcgct acttattaat tgtaattata tttattatag gttttagtgc attacctgta
     1741 tgggcaatgg actgctatgc tgaacatgaa gggggaaata ccgtagtcat tggttacgta
     1801 ccaagaattt ctatccccag taatggtaaa aaaggtgata agatctggca aagcagtgag
     1861 tattttatga atgtcttctg taataatgca ttacctgccc catcacctgg agaagaatac
     1921 ccatcagcct ggactaatat aatgatgttt ttaccaggcg gtcaggactt ttataatcag
     1981 aactcctata tttttggcgt aacatacaat ggagttgatt atgattcgac tgcccctaat
     2041 gctatcgcag caccagaatg cattgatata aaaggagcgg gtacctttaa taatcactat
     2101 aaaaaccccg ctgtctgtag tggaggtcct gagcctcaat tatcagtaac ctttccagct
     2161 cgcgtacaac tctatattaa attagcaaaa aatgccaata gagttaataa aaacctggtg
     2221 ttacctgatg aatatatcgc tctagagttt aaaggtatga gtggcgcagg agctatagaa
     2281 gtagacaaaa atttgacctt caggattcgt ggattaaata atattcatgt ccttgactgc
     2341 tttgttaatg tcgcactgga accagctgat ggcgttgttg actttggtaa aataaattcc
     2401 cgaacaatta aaaataccag cgttagtgag acgtttagtg tagtcatgac aaaagatcct
     2461 ggtgcggcct gtactgagca attcaatatt ttagggagct ttttcactac ggatattttg
     2521 agtgattata gccatctgga tatgggtaat ggactgctat tgaagatatt tcataaagat
     2581 ggaacagcaa cggaatttaa ccgcttctca caatttgctt ctttttcatc gtctagtgca
     2641 ccttcggtca ccgcaccatt cagggcagaa cttagtgcga acccggcaga gactgttgtt
     2701 gagggaccgt ttagtaaaga cgtaatcctc aaaatcacct at
//
LOCUS       DAJVXP010000255         2739 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVXP010000255 DAJVXP010000000
VERSION     DAJVXP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.259"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OQS10_004142"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OQS10_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCS2295009.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="OQS10_004143"
     CDS             1322..2374
                     /locus_tag="OQS10_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCS2295010.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAJVXP010000256         2721 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVXP010000256 DAJVXP010000000
VERSION     DAJVXP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.260"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            30..2546
                     /locus_tag="OQS10_004144"
     CDS             30..2546
                     /locus_tag="OQS10_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2295011.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="OQS10_004145"
     CDS             2557..>2721
                     /locus_tag="OQS10_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2295012.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAJVXP010000257         2712 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVXP010000257 DAJVXP010000000
VERSION     DAJVXP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.261"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(18..116)
                     /locus_tag="OQS10_004146"
                     /pseudo
     CDS             complement(18..116)
                     /locus_tag="OQS10_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(162..344)
                     /locus_tag="OQS10_004147"
     CDS             complement(162..344)
                     /locus_tag="OQS10_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2295013.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            443..1333
                     /locus_tag="OQS10_004148"
                     /pseudo
     CDS             443..1333
                     /locus_tag="OQS10_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1336..1869
                     /locus_tag="OQS10_004149"
     CDS             1336..1869
                     /locus_tag="OQS10_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2295014.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1898..2425)
                     /locus_tag="OQS10_004150"
     CDS             complement(1898..2425)
                     /locus_tag="OQS10_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS2295015.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2427..>2712)
                     /locus_tag="OQS10_004151"
     CDS             complement(2427..>2712)
                     /locus_tag="OQS10_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS2295016.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          755 a          608 c          595 g          754 t
ORIGIN      
        1 cggcataatc ccaatgatta ctccctgaca gggttcgtag gccactcaat atcaggtgca
       61 gttgatgtat caacacggtt cagcaacacc cgatactttt tctaggcttc caccaccagc
      121 acgacaagat gccgcataca gtgccccagt cagtccagtt ttcagacaac cagtgcgtca
      181 cctttttgaa ggcgctttaa agcacgtttt aatccaggtc ggcctgtcct tgttccgctt
      241 aatttatctt caaatatttg ttcatatcct gcacaaacaa gagcgtttcg ttgcaggtct
      301 gtattctggt catttgttga tacccttaca tagccaatca gcacgctgaa tctcccgtcc
      361 aaaagcacaa atcatgccat gcaggccaga aaccgccatt atctaaaacc tcggtttacg
      421 agaactcggc acaagcgggg agaaaatacc gttactcagt acagcgaata cctggactaa
      481 tcgacaaaca ttcagcggtg gcctttctgg tgaactatcc ggcaatgctt ctacagctgc
      541 aaaattaaaa actgccagga aaataagcaa tgtggctttt gatggttcct ccgatatcac
      601 attaaaagca agtcatgttg gtgcgtttgc cttagggaaa acaggaagca ccgttgcgaa
      661 tgataaagca gttggatgga actggagtag cggagcctat aacgcaacta ttagtggtgc
      721 atcaacgtta attattcatt tttatatggg agaaggaagt tgtcctgcag ctcagtttcg
      781 gattaattat aaaaatggcg gtatttttta tcgttcagcc cgtgatggtt atggttttga
      841 agccgactgg tccgaatttt acaccaccag aaaaccttca gcaggagatg ttggtgcact
      901 gccgttatct ggtggtcaac tgaatggtgc actgggtatc ggaacatcca gtgctcttgg
      961 cggtaattcg attgtattgg gtgataatga cacgggcttt aaacaaaaag gcgatggtaa
     1021 tctggatgtt tatgctaata acgtccatgt tatgcgcttt gtctccggaa gcattcaaag
     1081 taataaaacc ataaatatta cgggacgtgt taatccctcg gattacggta actttgattc
     1141 ccgctatgtg agagatgtca gacttggcac acgagttgtt tagaccatga agaaaggcgt
     1201 gatgtatgaa aaatcaggcc atgtaatcac ggggcttggt attatcggtg aagtcgatgg
     1261 tgatgatccg gcagtattca gaccaataca aaagttaatt aacggaacat ggtataacgt
     1321 atcgcaggta taatcatgca gcatttaaaa aacattgtcg caggtaatcc aaaaaccgtt
     1381 gaacaatacc agctaacaaa gaattttgat gttatctggt tatggtccga agatggaaaa
     1441 aattggcatg aggaattaaa aaactttcag gaggatacaa taaaactggc ttacacagtt
     1501 gagggaataa ttattgctat agacaaggat gtatcagcaa ttaatccaga aggtttaagt
     1561 gtcgttgagt tgcctgatat tacagcaaat cgccgggctg atatttcggg caactggatg
     1621 ttcaaagatg gtgcggtgat aaagcgaact tataccgagg aagagcaaag gcaacaagcg
     1681 gaaaatgaaa agcagagcct gctgcagctc gtcagggata aaacccagct gtgggactct
     1741 cagctacagc tgggcatcat ttccgatgag aataaacaaa aattaaccga gtggatgctc
     1801 tatgcgcaga aggttgaatc cacagacacc tccagcctgc cagtaacgtt tcccgaacaa
     1861 ccagaatgag agaaggcccg ctatcgggcc ttaattttta ttcaggcttt tgtggccatt
     1921 caggatttgc cgtatccaca cggctgacca gaacactgta gcgttcccat gcttccagtc
     1981 gtgtgcgttc ctcgtctgtt gccatattca gcctgacagc tcgttccagc ggctggatga
     2041 ctgattcagc ttcggaaagc aatgcggcct tttgtgattc ggcctgttgt tgcagttcct
     2101 gttctgtata ttccctcttt actactttac catccagaaa catccatttt ccgttattgt
     2161 ctgcgtgacg atttgccgta atatcaggaa cttcaactat gcttagccct tcaggattaa
     2221 tagttgaaac gtctttactg acagcaacga tgatgttgtt ctcatcgtaa gcaattttta
     2281 tggtgtctgg ctgaaaattc ttttgttcct cataccagtt tttgccatct tcgcaaaaga
     2341 gccataccac gcccgctttt ttagttagct ggtattgttc cggtgttttc ggattcccgg
     2401 ccttaatatt tttcaggtgc atcatcttaa acactcccca cgttgtacca tattccccca
     2461 atcaatttct gaattggcct tctggcaaca gtgtcgacaa tatcatccgc attgctgtta
     2521 attgcggctg ttaaaacata acctgacgca tcgccaaagc cgtagtcgcg ccatacgttt
     2581 ttatattcaa tgctgccaag acgaatatcc cgaacgacat gattatgcag ccatgtgctt
     2641 aaccagttgt tttcccacac tgaaccataa atatcgccac tagacgcaac ttgcgcacca
     2701 cttcccagcg ca
//
LOCUS       DAJVXP010000258         2690 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVXP010000258 DAJVXP010000000
VERSION     DAJVXP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.262"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            230..769
                     /locus_tag="OQS10_004152"
     CDS             230..769
                     /locus_tag="OQS10_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS2295017.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OQS10_004153"
     CDS             complement(995..1318)
                     /locus_tag="OQS10_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS2295018.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OQS10_004154"
     CDS             1604..1798
                     /locus_tag="OQS10_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS2295019.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJVXP010000259         2581 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVXP010000259 DAJVXP010000000
VERSION     DAJVXP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.263"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..1401
                     /gene="fimD"
                     /locus_tag="OQS10_004155"
                     /pseudo
     CDS             1..1401
                     /gene="fimD"
                     /locus_tag="OQS10_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1414..2421
                     /gene="fimH"
                     /locus_tag="OQS10_004156"
     CDS             1414..2421
                     /gene="fimH"
                     /locus_tag="OQS10_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCS2295020.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2432..>2581
                     /locus_tag="OQS10_004157"
     CDS             2432..>2581
                     /locus_tag="OQS10_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbriae assembly protein"
                     /protein_id="HCS2295021.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKT"
BASE COUNT          646 a          565 c          666 g          704 t
ORIGIN      
        1 aattatctgc caattacacc gcctttttat tagggctggc gcaacctcgg gaactggggc
       61 gcagtgtcgc tggatgtgac ccatgcgcgc agccagttag ccgacgacag tcgtcatgag
      121 ggggattcca ttcgcttcct ctatgcgaaa tcgatgtaca ccttcggcac caattttcag
      181 ttaatgggtt accgctattc gacacaaggt ttttataccc ttgatgatgt tgcgtatcgt
      241 cgaatggagg ggtacgaata tgattacgat tacgacggtg agcatcgcga tgaaccgata
      301 atcgtgaatt accacaattt acgctttagc cgcaaagacc gtttgcagtt aaatatttca
      361 caatcactta atgactttgg ctcgctttat atctctggta cccatcaaaa atactggaat
      421 acttcggatt cagatacctg gtatcaggtg gggtatacca gcagctgggt tggcatcagt
      481 tattcactct cattttcgtg gaatgaatct gtagggatcc ccgataacga acatattgtc
      541 ggacttaatg tttcagtgcc tttcaatgtt ttgaccaaac gtcgctacac ccgggaaaat
      601 gcgctcgacc gcgcttatgc ctccttgaac gctaaccgta acagcaacgg gcaaaatagc
      661 tggctggcag gcgttggtgg gaccttactg gaaggccaca acctgagtta tcacgtaagc
      721 cagggcgata cctcgaataa tgggtacacg ggcagcgcca cggcaaactg gcaggccact
      781 tacggtacgc tgggggtcgg gtataactac gaccgcgatc aacatgacgt taactggcag
      841 ctgtctggcg gtgtggtcgg gcatgaaaat ggcataacac tgagccagcc tttaggggat
      901 accaatgttt tgattaaagc gcttggcgca ggcggtgtac gcattgaaaa tcaaactggc
      961 attttaaccg actggcgcgg ctatgcggtg atgccgtatg ccacggttta tcggtataac
     1021 cgtatcgcgc ttgataccaa tacgatgggg aattccatcg atgttgaaaa aaatattagc
     1081 agcgttgtgc cgacgcaagg cgcgttggtt cgtgccaatt ttgatacccg cataggcgtg
     1141 cgggcgctca ttaccgttac ccagggcgga aaaccggtgc cgtttggatc actggtacgg
     1201 gaaaacagta ccggaataac cagtatggtg ggtgatgacg ggcaagttta tttaagtggt
     1261 gcgccattgt ctggtgaatt actggttcag tggggagacg gcgcgaactc acgctgcatt
     1321 gcgcactatg tattgccaaa gcaaagctta cagcaagccg tcactgttat ttcggcagtt
     1381 tgcacacatc ctcgctcata aaggaaatta tcaatgaaga taatctgcag attattattg
     1441 gcaatggcat gtttgtgtct ggcaaacata tcctgggcta ctgtttgtgc aaatagtact
     1501 ggcgtagcag aagatgaaca ctatgatctc tcaaatgtct ttaatagcac caataaccag
     1561 ccagggcaga ttgttgtttt accggaaaaa tccggctggg taggtgtctc agcaatttgt
     1621 ccactcggca cgctggtgaa ttatacatac cgtagttatg tcactaactt tattgttcag
     1681 gaaactatcg ataattataa atatatgcaa ttaaatgatt atctattagg tgcgatgagt
     1741 ctggttgata gtgtgatgga tattcagttc cccccgcaaa attatattcg gatgggaaca
     1801 gatcctaacg tttcgcaaaa ccttccgttc ggggtgatgg attctcgttt gatatttcgt
     1861 ttaaaggtta ttcgtccctt tattaacatg gtggagatcc ccagacaggt gatgtttacc
     1921 gtgtatgtga catcaacgcc ttacgatccg ttggttacac ctgtttatac cattagtttt
     1981 ggtggccggg ttgaagtgcc gcaaaactgc gaattaaatg ccgggcagat tgttgaattt
     2041 gattttggtg atatcggcgc atcgttattt agtgcggcag ggccggggaa tcgaccagct
     2101 ggcgtcatgc cgcaaaccaa gagcattgcg atcaaatgta cgaatgttgc tgcgcaggct
     2161 tatttaacaa tgcgtctgga agccagtgcc gtttctggtc aggcgatggt gtcggacaat
     2221 caggatttag gttttattgt cgccgatcag aacgatacgc cgatcacgcc taacgatctc
     2281 aatagcgtta ttcctttccg tctggatgca gctgcggcag ccaatgtcac acttcgcgcc
     2341 tggcctatca gtattaccgg tcaaaaaccg accgaagggc cgtttagcgc gctggggtat
     2401 ttacgcgtcg attatcaatg aggtacggag aatgagaaga gtactcttta gctgtttctg
     2461 cgggttactg tggagttcca gtggatgggc agctgaccct ttgggaacga ttaatatcaa
     2521 tttgcacggt aacgttgttg atttctcctg taccgtaaac actgcggata ttgataagac
     2581 g
//
LOCUS       DAJVXP010000260         2579 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVXP010000260 DAJVXP010000000
VERSION     DAJVXP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.264"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..221
                     /locus_tag="OQS10_004158"
     CDS             <1..221
                     /locus_tag="OQS10_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2295022.1"
                     /translation="LPAIRGKGLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALY
                     EHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(267..683)
                     /gene="rraB"
                     /locus_tag="OQS10_004159"
     CDS             complement(267..683)
                     /gene="rraB"
                     /locus_tag="OQS10_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS2295023.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            845..1849
                     /gene="argF"
                     /locus_tag="OQS10_004160"
     CDS             845..1849
                     /gene="argF"
                     /locus_tag="OQS10_004160"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS2295024.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(1921..>2579)
                     /locus_tag="OQS10_004161"
     CDS             complement(1921..>2579)
                     /locus_tag="OQS10_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2295025.1"
                     /translation="YFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFFN
                     LDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSILV
                     NDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGYV
                     VKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWNN
                     "
BASE COUNT          712 a          595 c          543 g          729 t
ORIGIN      
        1 ttcttccggc tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc
       61 aggcacgaga gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag
      121 ccattgcgct ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg
      181 gccatgtcga ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg
      241 tgctatcggg cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca
      301 aaatcttcat cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg
      361 tactcaacgt caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga
      421 tcggcattca acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca
      481 acttccagct cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct
      541 ttttccagag tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc
      601 ggatcgctgc catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct
      661 tccagttgtt ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt
      721 gtcacacacc actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca
      781 aatgaataat catccatata aattgaattt taattcatta aggcgttagc cacaggaggg
      841 acctatgtcc gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga
      901 actcaacagc ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga
      961 agaagccaaa ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac
     1021 ccgatgctct ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc
     1081 aagcggcagc caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg
     1141 catgtatgac ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga
     1201 atacgctggc gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct
     1261 ggcggatctc ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct
     1321 agtctatgca ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct
     1381 taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt
     1441 tacggaatgc cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt
     1501 agctaaagga gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga
     1561 agcaaaagag aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa
     1621 gatgatgcag ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca
     1681 tgacgaccaa acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat
     1741 ggaagtgact gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa
     1801 ccgcatgcat accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct
     1861 ataaagatgc cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt
     1921 tcaattattc caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt
     1981 tcctaactgt tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt
     2041 catcattctt gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac
     2101 ataacccgga agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc
     2161 taactcatta agatacagct gttcgccctc ataacactta agtaattgat agaagtacca
     2221 cttagcaaaa tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat
     2281 gctgacaggc acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg
     2341 tgatttttct tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat
     2401 caattcctgt aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt
     2461 ctcagctatt ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa
     2521 atcaatatga tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatatt
//
LOCUS       DAJVXP010000261         2557 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVXP010000261 DAJVXP010000000
VERSION     DAJVXP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.266"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..257)
                     /locus_tag="OQS10_004162"
     CDS             complement(<1..257)
                     /locus_tag="OQS10_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCS2295026.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIR"
     gene            complement(340..1386)
                     /gene="mdtG"
                     /locus_tag="OQS10_004163"
                     /pseudo
     CDS             complement(340..1386)
                     /gene="mdtG"
                     /locus_tag="OQS10_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(1717..2292)
                     /locus_tag="OQS10_004164"
     CDS             complement(1717..2292)
                     /locus_tag="OQS10_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2295027.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          689 a          594 c          596 g          678 t
ORIGIN      
        1 cggatctcta tctcatcata gtcgccatca aaaacacttt gcgcggcttc accgctaagc
       61 ataggtaaac attcaccttc ttccccttcg tcggcaaaaa actcaacttg ccacatgatg
      121 tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt gcacattcgc atcttcggcg
      181 tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag cgtcaatggc ttcttcaagc
      241 gttgcgtaca tggtcatcac gattctccct ttgagttgat gaggtttcag ggaaaaggat
      301 agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat cagttcgata cctggggtat
      361 tcgacgacga cgtagactgt tccatgaata gactgcgttg aataacacta cgccagcggt
      421 gacgagaaat accgctctga aaccgtagtt cgctgaaatc gctgctccca tcaatggtcc
      481 ggtaacgttg ccaatatcac gaaacgattg gttatagctg aagatacgtc cggcgatttg
      541 gttgctcgag ttataaacta acagtgtctg tacggcgggg agtagtgcac catcggcggc
      601 accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc tgaacgtaag acattgggat
      661 caacagcagt acagaaaaga tcagcgccgt aatcaggatc ttttcgggtc cgattcgatc
      721 gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc acgcctggca ccgaggcgat
      781 catgccactg ataaaggcga cgttactgac gttacccgcc agttcgcgga catacagcgt
      841 cagaatgggg gcaattgagc ccgtcgccac ctggatgatt aacgtagtga caaacaggct
      901 gagtaccagt ttcgggtttt taagtgatgt caccacttcc cgcatgtgca gcatctcttt
      961 tttgctgatc ggctggaatt tttctcttat gcaaaacagg gtgacgaaaa agcagagtat
     1021 gagcacactg gcggtaataa agaataccgg acgtaagccg tagctatcgg cgagcaggcc
     1081 gccagccatt gggccgagca acgcaccact aacgccgcct gtggagagcg tacccagcgc
     1141 ccagccgctt ttattacgcg gtacttgtgt ggcgataaga gcattagcgt tggggacaaa
     1201 tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac tgccagatat tttgtgccag
     1261 ccccatcaac accatcacga tgcccatgcc gagggcagag cgtaatagca tgagttttcg
     1321 gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg atggccgaaa ataaaaatgt
     1381 aatgctcata tatgctatct taatgcacta cctgataagt gagcctatac aggttattgc
     1441 tgtatgttta tacagtatta ttggtagaag actgtgtaga tttggtatag agatgaatag
     1501 atactattaa ggtaacttat tgaaatcaat aaagattctt gtgcagtctg aatagataac
     1561 ttagcgttat gtctagtcgt ttccctctcc attcctaaaa agtgctgtat actggcctat
     1621 acaacgctat aaagacctct acatagcgcc ttgatagcta tgtcacaggt acaaaaaagc
     1681 cccctataaa ggaggctgtg tagtctatta agggtcttac agggtcttgg tccagtgtct
     1741 gctaacagtc cttacagaga ggttaagaac cttagctacc tgagactgag atagtcccct
     1801 ttccttgtac tctaagaccg ctctagttgt gtcactcgct ataggtcttc caagtacctt
     1861 accttgaact ctagcacgct ctagaccctg tttagtgcgc tctctgatac ggtttctttc
     1921 aaactctgca aaggtactaa atagctgtag aaccaactta ccctctgagc ttgaaaggtc
     1981 tttaacgggt aaatctaaac agatgagctt cacaccctta tcattaagaa gggtcactgt
     2041 attctgtagg tcgatgttat cccttcccag acggtcaagt ttaagaacaa caagggtgtc
     2101 accttcttct agcttatggt tgaccagatt cgcaaatccg tccctatcca tagctgacac
     2161 tgaaccagag acaacctcct ctataactcg tgacttagga atatcatagc cagccttctt
     2221 aatggcttga atctggttct ctgtggtgga agtctctact gatacacggc agtaagcaaa
     2281 ggttcgtgac atagatatcc ctctgggtag tcattaaacg gttagtttgt cttgtctata
     2341 gacattaaag caaatggtag atttgttgtc tatagctata gtgactcttc tatgtagaca
     2401 ttaaacggac gtttcttgtc gtgggtcaca tagatacttt tagggcttct gtcaagtgat
     2461 attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca gggctactaa gaacacatac
     2521 atctaaggct ggaaagtacc tgagcagata ttcttca
//
LOCUS       DAJVXP010000262         2452 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVXP010000262 DAJVXP010000000
VERSION     DAJVXP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.267"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(123..1541)
                     /gene="phrB"
                     /locus_tag="OQS10_004165"
     CDS             complement(123..1541)
                     /gene="phrB"
                     /locus_tag="OQS10_004165"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS2295028.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1538..2047)
                     /locus_tag="OQS10_004166"
     CDS             complement(1538..2047)
                     /locus_tag="OQS10_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS2295029.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          621 a          631 c          560 g          640 t
ORIGIN      
        1 ggagtcatta ccttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt
       61 gtaggctgga taaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc
      121 tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg
      181 tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac
      241 ggctcatgca ccgatttccc cggtacattg cgtagttccg gtagccactg gcggataaac
      301 tcaccttcga gatcaaattt ctcgccctgg gttgtcgggt tgaaaatacg aaaatacggc
      361 gctgcatcgg ttccggttga agcggcccac tgccagccac cgttattggc tgccaaatca
      421 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caataaatct
      481 ttcaccagaa aactggctgt aatcatccgt agcctgttat gcatccagcc agtgctgtta
      541 agctgacgca tagcggcatc aacaatcggg tatcccgttt tgccttcctg ccaggcctgt
      601 aaatgtgcgg gattgctctg ccactgtaca cgatccgtcc aggcaataat tggacgatgt
      661 ttacacaacg aggggtgata cgttatcagg tgacggtaaa actcgcgcca gatcagctca
      721 ttaagccaga cactaccgac cccaccgtcc agcgcctgcg gctgttcagc caacaagcga
      781 tgcaagcact ggcgaggcga taacccgccc gttgccaggc tggccgacaa acggctggtg
      841 ccttccactg ccggaaaatc tcgttgttgc tcatattctc cggcaccgtt ctggcaaaac
      901 tggcgtaatt gcgcaatcgc cgctttttct tccaccggaa aatgcgcagt atcgaaagac
      961 tgacgaggat aattcagcgt aatggatggc gagggctcta tcgatccgct actacgaact
     1021 tttggcgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
     1081 aaaggcgtaa agactttgta catctcgtga ttaccggtca tcaccgcgcc aggcggcagg
     1141 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacttccaca
     1201 tcccgcgccc gctcattcac ttcatactga tagttataaa acaggtgggt aacgctgttt
     1261 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
     1321 aataaaggaa tacctttttc cgcaagcgct atttgtagcc cattcagttg agcattgata
     1381 agttcagcct gacgcggcga catgttatgc gtcgcccact ggcgtggtgt agcgatatac
     1441 aacgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
     1501 agacgtaaat cttggcgaaa ccagaccaga tgggtagtca taaaactcct gaatggcgca
     1561 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcatgat
     1621 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
     1681 gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa
     1741 aataaccctg aacgtgcatc aaaacattgg tgtgatcgcg cgggttggcg gggtgagaaa
     1801 gcaaaacaac tacgcgttgg cggtactggt tgtaatagtc atccagattt tgccactggt
     1861 gaatatcagc cacgaacggg ccaagtttgc ggtactccgg ctgagaatgc gccagaaaga
     1921 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
     1981 gtcgatgtaa ctcatgaagg gcaaagatac ggattagggt gctgtcatca aatcgttgta
     2041 gattcattac tgctcctgaa cggtataaag gagtaagtgt agacgatggt aaatgaattt
     2101 tatggggcag agtttttgcg gggaagtatt ttgttggtaa aaatattatc aacttcatct
     2161 gagtgagaat tattctacta tttcccgagc tgcattcatt ttttgtattt tttattttta
     2221 gaaaataaca gttctcaaag cttaattacc tcactattaa ttgtatttca aactaacaat
     2281 cgcactccat tttctctttt taaaaaacat aaacactgtt aaaacaaata cttatttcgt
     2341 tatcttaaat gctatttcta catcactcgt ccttataatc aaattattct tattcatagc
     2401 tatttttgca ggtttaaaat agcggctttc accaaagtca gccatgaaaa tt
//
LOCUS       DAJVXP010000263         2433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVXP010000263 DAJVXP010000000
VERSION     DAJVXP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.268"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            523..2376
                     /locus_tag="OQS10_004167"
     CDS             523..2376
                     /locus_tag="OQS10_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS2295030.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
BASE COUNT          595 a          608 c          677 g          553 t
ORIGIN      
        1 catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa tgctatgttg
       61 ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa aaaaatttcg
      121 catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct gattatcaaa
      181 acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca cccggcacca
      241 tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt tcttgtgggc
      301 aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa cgtgaagtaa
      361 tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg cggtcttttt
      421 cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga cttcagcccg
      481 ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt taaacactat
      541 gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca aaaactgaag
      601 gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct gatgcaggcg
      661 attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg gtactacgtc
      721 atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc gcttccggat
      781 tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac agtgactccc
      841 ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct gcatgatgtt
      901 caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca gtacggctgt
      961 gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa taatgcgggg
     1021 atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac ggaggggaca
     1081 ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg taagccgtta
     1141 tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa aaacgttttg
     1201 ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta cgcccagcag
     1261 ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg gctggcggcg
     1321 cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac gacatacgcg
     1381 tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa aggtaaagaa
     1441 tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa tacaccgaca
     1501 aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc ggcatcccga
     1561 acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg ggcgcgtcgt
     1621 ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg caccttagcc
     1681 ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac gccatcgggg
     1741 ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg agacgctgct
     1801 gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt atttaagatc
     1861 accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc agtctccctg
     1921 ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact gaccaacaac
     1981 cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt cgtggcgatg
     2041 accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga cggcaaactg
     2101 aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg gaattacagt
     2161 aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt tactccgaaa
     2221 ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac actggggctg
     2281 aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga gatagaaagt
     2341 ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc aaatggaagg
     2401 tatcgttaaa ctcaccggta gtgtcagtgg gtc
//
LOCUS       DAJVXP010000264         2271 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVXP010000264 DAJVXP010000000
VERSION     DAJVXP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.269"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..431)
                     /locus_tag="OQS10_004168"
     CDS             complement(<1..431)
                     /locus_tag="OQS10_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS2295031.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            488..757
                     /locus_tag="OQS10_004169"
                     /pseudo
     CDS             488..757
                     /locus_tag="OQS10_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            872..1042
                     /locus_tag="OQS10_004170"
     CDS             872..1042
                     /locus_tag="OQS10_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295032.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(1169..1726)
                     /locus_tag="OQS10_004171"
     CDS             complement(1169..1726)
                     /locus_tag="OQS10_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS2295033.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1723..1998)
                     /locus_tag="OQS10_004172"
     CDS             complement(1723..1998)
                     /locus_tag="OQS10_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS2295034.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            2183..2271
                     /locus_tag="OQS10_004173"
                     /pseudo
     CDS             2183..2271
                     /locus_tag="OQS10_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          647 a          468 c          534 g          622 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tagtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gccgcagaca
      781 cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg ttgttatttt
      841 tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg tggtgatgtc
      901 agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag atcaccaggt
      961 aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg atgaggttga
     1021 tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga ataacctgaa
     1081 tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat aaaattaacc
     1141 tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc accaattgtt
     1201 ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt tccagagtgc
     1261 ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt ttatcgaagt
     1321 ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca ggaggacgtt
     1381 tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt agtctggctg
     1441 cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg atcaggtagc
     1501 atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg gccgaaaagt
     1561 tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa acgtgtttat
     1621 gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga ccatctttga
     1681 aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt gatgactcca
     1741 attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac gtacagggtt
     1801 ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac gccccaccat
     1861 aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat gcgttgtgat
     1921 gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa tatagccccg
     1981 gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta taaaattaaa
     2041 gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa atcaggcagt
     2101 taaaaacacg gaaaacggta taaaaaagcg cctcaaaatc ctgttttttc tttcagatac
     2161 aatgtcttat tgcatttttt aaaacatgtc agtgtggtac atggatatcg ataccatcgc
     2221 caattataaa ctgggcggtg cacagcaaca tgatagcgta cgcctcgatc c
//
LOCUS       DAJVXP010000265         2249 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVXP010000265 DAJVXP010000000
VERSION     DAJVXP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.270"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..161
                     /gene="ydcY"
                     /locus_tag="OQS10_004174"
     CDS             <1..161
                     /gene="ydcY"
                     /locus_tag="OQS10_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HCS2295035.1"
                     /translation="LLIALSDDAELSREDRYTQQQRLRTAIAHHGRKHKEDMEARHEQ
                     LTKGGTIL"
     gene            complement(162..611)
                     /locus_tag="OQS10_004175"
     CDS             complement(162..611)
                     /locus_tag="OQS10_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS2295036.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(608..1126)
                     /gene="mddA"
                     /locus_tag="OQS10_004176"
     CDS             complement(608..1126)
                     /gene="mddA"
                     /locus_tag="OQS10_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS2295037.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1307..>2249
                     /gene="curA"
                     /locus_tag="OQS10_004177"
     CDS             1307..>2249
                     /gene="curA"
                     /locus_tag="OQS10_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS2295038.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDI"
BASE COUNT          585 a          561 c          604 g          499 t
ORIGIN      
        1 aattattaat cgccctgagc gatgacgcgg agttaagtcg ggaagatcgc tacacccagc
       61 agcagcgttt gcgcaccgct attgctcatc acggtcgcaa gcataaagaa gatatggaag
      121 cgcgccacga acagttaacc aaaggcggca ccatcctctg attaaaatga gcgtctggct
      181 accaaccagg cgccaaccac aagtaaaatt gcgccacaaa ttgggccgaa caacgcccgt
      241 agtggtacgc catgactgcg gaagatatcc agcatcagac cgccaattag ctggctggcg
      301 accagtactg caatagttgt cgccgctccc acattctgat atccgctgat actggcgaag
      361 acaaaaaacg atcccagcaa gccgggtata agtgtccacc agcgcacgct ggacaccagt
      421 tcgccaaacc ccgccatgcc ttgtttaaac cataaaatag aaacaaagag cacaatcccc
      481 accagtgagt tcagcaacat ggcgatgaga atggtagagg aggtctgggt gatgcgcacc
      541 atcagcgtgt tctggactac cagaccaatt ccggcggcaa tcaaaaaagc aagggtgagc
      601 gactgattca tccaatcgcg tccggttcag tgcgttcgtc gagttgcaac tgcataaatg
      661 tcagatccag ccagcgccca aatttagtgc ccacctgcgg catttgtgcg gtgacgacaa
      721 atcccagcga atggtgcaga tgcagcgagg cctgattttg cgattcgatc ccggcgacca
      781 tgacatgctt cccgcaatcc cgcgcttcat caatcaatcg gcttaacaat ttacgcccca
      841 gacctttgcc ctgatgatcg ggatggacat aaaccgaatg ttccacggta tggcgaaaac
      901 catcgaaact acgccagtcg ccaaacgagg catatcccgt cactacgcca tcttcttcac
      961 ttaccagcac cggataacct gctatggtcc gcgcttcaaa ccaggcaatg cggttatctg
     1021 catcaaccgt ttggtcattc cagatagccg ccgtatacaa cacggcgtgg ttataaattt
     1081 ccgcaatggc agcacaatcg gctttgcggg caaaacggat ggacatgtct ggcctcgata
     1141 aaataatgat gatgatgtta tcaatactat cgaaaacagc ccgaagcaac ggattcctcg
     1201 atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc aatcccgtga
     1261 gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg ggcaacaaaa
     1321 gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg ttccggagaa
     1381 tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt tactgcgcac
     1441 agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc catcttattc
     1501 accgcctgtt gatattggcg gcgtgatggt cggcggcacg gtgagccgtg ttgtggagtc
     1561 gaatcatcct gattatcagc ctagcgactg ggtgctgggc tacagtggat ggcaggacta
     1621 tgacatatcc agtggtgatg atctggtgaa acttggcgat catccgcaaa atccatcgtg
     1681 gtcgctgggt gtgctgggga tgccaggctt taccgcttat atggggctgc tggatatcgg
     1741 tcagcctaaa gagggcgaaa cgttggtggt agctgcggcg acaggtcctg tgggagcaac
     1801 ggtggggcaa atcggcaaac ttaaaggttg cagggtggtg ggggttgccg gtggcgcgga
     1861 aaaatgccgc catgctatcg aggtgctggg cttcgatgtt tgtcttgatc accacgcgga
     1921 tgattttgcc gaacaactgg tgaaagcgtg cccaaaaggt attgatatct attatgaaaa
     1981 cgtgggcggt aaggtatttg atgcagtgct accattactt aatacatctg cgcgcattcc
     2041 cgtttgtggc ttagtgagca gctataacgc tacagagcta ccacccggtc cggatcgttt
     2101 acccctgttg atggcaacgg tgctgaaaaa acgtattcgc ttgcaagggt ttattatcgc
     2161 tcaggattat ggtcaccgca tccatgagtt tcagaaggag atggggcaat gggtgaaaga
     2221 ggataaaatc cactaccacg aagatatta
//
LOCUS       DAJVXP010000266         2241 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVXP010000266 DAJVXP010000000
VERSION     DAJVXP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.271"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..312)
                     /locus_tag="OQS10_004178"
     CDS             complement(<1..312)
                     /locus_tag="OQS10_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCS2295039.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQS10_004179"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OQS10_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS2295040.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
BASE COUNT          592 a          625 c          525 g          499 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc c
//
LOCUS       DAJVXP010000267         2195 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVXP010000267 DAJVXP010000000
VERSION     DAJVXP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.272"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="OQS10_004180"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="OQS10_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS2295041.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..>2195)
                     /locus_tag="OQS10_004181"
     CDS             complement(1188..>2195)
                     /locus_tag="OQS10_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCS2295042.1"
                     /translation="ELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLL
                     GFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPL
                     PPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPT
                     SRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLV
                     TPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDR
                     ALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKL
                     A"
BASE COUNT          627 a          478 c          469 g          621 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattc
//
LOCUS       DAJVXP010000268         2135 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVXP010000268 DAJVXP010000000
VERSION     DAJVXP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.274"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            34..120
                     /locus_tag="OQS10_004182"
     tRNA            34..120
                     /locus_tag="OQS10_004182"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Leu,seq:cag)
     gene            complement(159..395)
                     /locus_tag="OQS10_004183"
     CDS             complement(159..395)
                     /locus_tag="OQS10_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS2295043.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            536..1324
                     /gene="fhuF"
                     /locus_tag="OQS10_004184"
     CDS             536..1324
                     /gene="fhuF"
                     /locus_tag="OQS10_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS2295044.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1362..2039)
                     /gene="bglJ"
                     /locus_tag="OQS10_004185"
     CDS             complement(1362..2039)
                     /gene="bglJ"
                     /locus_tag="OQS10_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS2295045.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1997..>2135)
                     /locus_tag="OQS10_004186"
     CDS             complement(1997..>2135)
                     /locus_tag="OQS10_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCS2295046.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          558 a          558 c          476 g          543 t
ORIGIN      
        1 acgaggcgat atcaaaaaaa gtaagatgac tgtgcgaagg tggcggaatt ggtagacgcg
       61 ctagcttcag gtgttagtgt ccttacggac gtgggggttc aagtcccccc cctcgcacca
      121 attatcttta cttcctttct tgtttcttcc ttgatttttc atccctgatt caaattcatt
      181 atcatcagca tgatggcagc aataagcgcc aggcacgatg gtagcaaaac gtaatgccgt
      241 aactgtttgt gatacagcat caccggcgtc aatactaatc ccatcagaat gacaatccgc
      301 cactgatccg gcgataaatc cgcatacatc agccctgcaa tcagcaatcc cggcagcaac
      361 actccccagc cactgcccag cgtacgttgc aacatggttt catctctttc attgataatg
      421 ataaccaata tcatatgata atttttatca tttgcaagcc agagaaatcc cttgctatcg
      481 ggtaaaccta tcgctaggat tagcaatcat tatcatttag attactatcc cgattatggc
      541 ctatcgttcc gcaccgctct atgaagatat catctggcga acgcatctcc agccgcagga
      601 tgctgggctt gcacaagccg tacgtgcgac gatcgccaaa catcgtgaac atttgctcga
      661 gtttatccgc ctggatgaac ccgctccgct taacgccatg acgctggcgc aatggtcatc
      721 accgaatgcg ctaagttctc tactcgcggt ctattccgat catatctatc gcaaccaacc
      781 aacgatgatc cgcgagaaca aaccgctgat ctcactatgg gcacaattgt atatcggcct
      841 gatggtgcca ccattaatgc tggcgctact gacgcaggaa aaggcattag atgtgtcgcc
      901 ggaacatttc catgctgagt ttcacgaaac cggacgcgtc gcctgtttct gggtcgatgt
      961 gtgtgaagat aaaaacgcaa caccacattc gccgcagcag cgaatggaaa cgttaatcag
     1021 ccaggcgctg gttccggttg tgcaggcact agaagcgacc ggagagatca acggcaaact
     1081 tatctggagt aataccggtt atttgatcaa ctggtatctc actgagatga agcaactgct
     1141 cggcgaggcg acggttgaat cgctgcgcca tgccctcttt tttgagaaaa cgctcacgaa
     1201 cggtgaagat aatccactct ggcgtaccgt cgtgctgcgc gatggcctgc tggtgcgccg
     1261 aacctgctgc cagcgttatc gcttaccgga tgtgcagcaa tgtggcgatt gtacgctgaa
     1321 atgaaagcac tgccggggaa gtaaacccgg catcatgcgg attaataggg atgcaacaca
     1381 ttacttgctt cgcaatgccg catacataac agaatatctg cggcctccaa caaccctgca
     1441 tcagaactga cgcccagttt cgacatcaca ttaaatttat gtgcacggat cgttttgata
     1501 ttgcgtttaa gctgctcggc aatttgtgtc attgagtagc cacgcgacat aaagcgcaat
     1561 atttctctct ccgtcgggct tagcgtccgg ctttggttaa tgtaccactg attgttcagt
     1621 cggtcggtcg cctgacgtac accatttaat gacaaaaaga gttcctgatg aaaaatctcc
     1681 agcgttgacg ctttacttaa tacaccgtcc agcggtgatg gcgacaatga accaatcagc
     1741 cgagcttcaa tatcatcatc cgcaataact aaacgccggg tacgcggaaa cttaatcgcc
     1801 agttcagtca ggcaagataa tccctctctg cgctcacttc tcatggcaga aaaagaaaaa
     1861 atgaccgccg caaatgagat ccgcgacatg gcatcctgaa atgactcctg actggtatag
     1921 gtatggagct tataagggtt acccgcaaac tttctgaata aactctcaat accgatactg
     1981 ctcatgacgc atttttctat gagtgcgaca tttctcttct taattcggct gtgttccatt
     2041 ctgcggcatc tccataaaca gaaggcaagt taagttctct tgcaccctgc gcgctattta
     2101 tccatgcata catttcagcg ttgctgtgaa ttgcc
//
LOCUS       DAJVXP010000269         2135 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVXP010000269 DAJVXP010000000
VERSION     DAJVXP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.275"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..389
                     /locus_tag="OQS10_004187"
     CDS             <1..389
                     /locus_tag="OQS10_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295047.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OQS10_004188"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OQS10_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS2295048.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OQS10_004189"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OQS10_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS2295049.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OQS10_004190"
     tRNA            complement(1806..1881)
                     /locus_tag="OQS10_004190"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="OQS10_004191"
     tRNA            complement(1890..1966)
                     /locus_tag="OQS10_004191"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          555 a          516 c          513 g          551 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggcttactgc ttaatttgat gcctggcagt ttatgaatca
     2041 cttcgggctg ttgcttcgca acgttcaaaa gctttcgctt ttgtcctact ctcttatcga
     2101 gtagtgaact gcctcaaagg tcagtgccat tcttc
//
LOCUS       DAJVXP010000270         2093 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVXP010000270 DAJVXP010000000
VERSION     DAJVXP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.276"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            93..629
                     /gene="sutR"
                     /locus_tag="OQS10_004192"
     CDS             93..629
                     /gene="sutR"
                     /locus_tag="OQS10_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCS2295050.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            702..>2093
                     /gene="rlhA"
                     /locus_tag="OQS10_004193"
     CDS             702..>2093
                     /gene="rlhA"
                     /locus_tag="OQS10_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS2295051.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKL"
BASE COUNT          521 a          511 c          540 g          521 t
ORIGIN      
        1 tagtcacctc gtgcgttata gcgtacaggc accgtagcat ttgtccgtta taacgcacaa
       61 gtgataaact tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc tttttatcca
      121 ctacacttaa acaactacgc cagcagcgcg gctggagtct ttcgcgactg gcagaagcga
      181 cgggcgtttc taaagcaatg ttagggcaga ttgagcgtaa tgagtccagt ccgacggtcg
      241 cgacattatg gaaaattgcc accgggttga acgtaccatt ttcaacattt atttctccgc
      301 cgcagtccgc tatgccttca gtttatgatc cacaacagca ggcgatggtg atcacttcgc
      361 tgtttcctta tgatccacaa ctctgctttg aacacttctc gattcagatg gcatctggtg
      421 caataagcga atcgacgccc catgagaagg gggtaatcga acatgtcgtc gtcattgatg
      481 gacaacttga tctgtgcgtt gatggggaat ggcagaccct taattgtggg gaaggcgttc
      541 gatttgctgc agacgtcacg catatctatc gtaatggtgg ggagcaaacc gtacattttc
      601 attccctcat ccattacccg cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg
      661 tggctaaaat agccgccatt tttcagctac tggataagaa tgtgaccgta tcttctcatc
      721 gacttgaact gttaagcccg gcacgcgatg ccgccattgc ccgcgaagct attttgcacg
      781 gtgccgatgc tgtttatatc ggcggccctg gttttggtgc ccgtcataat gccagtaata
      841 gcctgaaaga tattgccgag ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg
      901 tcacgcttaa caccattttg catgatgatg agctggaacc cgcgcaacgg ctgattactg
      961 acctctacca gaccggtgtc gatgcgctga ttgttcagga tatggggatt ctggaacttg
     1021 atattccgcc gattgaactg cacgccagta cgcagtgcga cattcgtaca gttgaaaaag
     1081 cgaagttcct ctctgatgtt ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg
     1141 atcagatccg cgcgattcac caggctacgg acgcgaccat tgaattcttt attcatgggg
     1201 cactgtgcgt ggcctattcg ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg
     1261 ccaaccgtgg cgattgctcg caggcgtgcc gtttgccata cacattgaaa gacgatcagg
     1321 ggcgggtggt ttcctatgaa aaacatctgc tgtcgatgaa agataacgat cagactgcca
     1381 acctcggcgc gctgattgat gctggcgtac gctccttcaa gattgaaggg cgttacaaag
     1441 atatgagcta cgtgaagaat atcaccgccc attatcgcca gatgctggat gccattattg
     1501 aagaacgtgg cgatctgacg cgtgcttcat caggtcgtac tgaacatttc tttgttccat
     1561 cgacggaaaa gactttccac cgtggtagca cagattattt tgtgaatgcc cgtaaaggcg
     1621 atattggcgc gttcgattcg ccgaaattta tcggcctgcc ggtgggtgaa gtattgaaag
     1681 tggcgaaaga tcatctcaat gttgccgtca ccgagccact ggcaaatggt gatggcctga
     1741 acgtgatgat taaacgtgaa gtcatcggtt ttcgtgccaa tacggtcgag aaaaccggag
     1801 aaaatcagta ccgcgtctgg cccaatgaaa tgccagcaga tttgcacaaa attcgtccac
     1861 atcacccact aaaccgtaat cttgatcata actggcagca ggcactgaca aaaacctcca
     1921 gcgaacgtcg ggtggcggta gacattgaac tgggcggctg gcaggaacaa ctgattctga
     1981 ccctcaccag tgaagagggt gtcagcatca cgcatacgct ggacgggcag ttcgacgaag
     2041 ccaataacgc cgaaaaagca atgaacaatc tgaaggatgg tctggcaaaa ctg
//
LOCUS       DAJVXP010000271         2089 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVXP010000271 DAJVXP010000000
VERSION     DAJVXP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.277"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(60..1025)
                     /locus_tag="OQS10_004194"
     CDS             complement(60..1025)
                     /locus_tag="OQS10_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCS2295052.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OQS10_004195"
     CDS             complement(1879..>2089)
                     /locus_tag="OQS10_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295053.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAJVXP010000272         1928 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVXP010000272 DAJVXP010000000
VERSION     DAJVXP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.278"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..106
                     /locus_tag="OQS10_004196"
     CDS             <1..106
                     /locus_tag="OQS10_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS2295054.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
     gene            103..1494
                     /locus_tag="OQS10_004197"
     CDS             103..1494
                     /locus_tag="OQS10_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS2295055.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            1491..1748
                     /locus_tag="OQS10_004198"
     CDS             1491..1748
                     /locus_tag="OQS10_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295056.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            1748..>1928
                     /locus_tag="OQS10_004199"
     CDS             1748..>1928
                     /locus_tag="OQS10_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295057.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIA"
BASE COUNT          500 a          437 c          562 g          429 t
ORIGIN      
        1 gttcatcagc ccccgcattg ccgacaggat cacagacggg gggcgcaact ggttgtcgtt
       61 caactggccc agctaccgtt cttgtatcag aggtgtggct gcatgacaac accagtctgg
      121 cgtaatgatg acctggaagg cgctgtcatt ggcgcattct ttctgcgtgg ggcagatcat
      181 gaagtgatgg atattctgat cacactaccg gcggacattt tttctgtacg agcgtatcgg
      241 gatatctaca caggcatctg cagacaggcc cgtgtttcag gagtgattga ccccgtgctg
      301 ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg taaaacctgg
      361 gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc actgcgcgat
      421 gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac ctgtgaagca
      481 gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac aggaaagggc
      541 gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg tgttgaatgc
      601 cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga actggacgca
      661 aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc ttcgatgggg
      721 aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca tggtgtgctt
      781 ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc tgcggctgga
      841 gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga ctgggcgcgt
      901 ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga ccaggcgaac
      961 ctgaccattg atgagatatg cgcaacaacg aaacaccatc tgattaaaca tccggaaacg
     1021 gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg gcgtcatgac
     1081 cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg tggttttccg
     1141 ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa acgtcccatg
     1201 aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat tctgatgctt
     1261 tacagggatg aagtgtacaa cccggataca caggctacag gcatagcaga aattaatatc
     1321 acgaaacaac gtaacggttc tctggggacg atttaccggc gtttttataa cggacatttt
     1381 ctgcctgtgg accaggaaag cgcacaggtt ctttccaccc caatgcggca gccccagccg
     1441 cgcagataca gcaatacacg taccgacagc agtaagatgg agcgtttctt ttgaacaacc
     1501 agacaatgac ttttacctct gaacaattgc gtaaacacgc gcaggaaatg ttgcgacagg
     1561 cggaacaact ggaaaaaaca ggtgtaacaa aagatgccat tcgtcgggat atggtgccag
     1621 cgctcaggga actgatgcag gcgaaacatc gcgcacaaaa agcggtggat gagctcgttg
     1681 attgtgtggc agagctggaa accaaagttg gaaagtttga aaaaatggtg caggaggtgc
     1741 tgcgctgatg cgccatgagt ttattttacc ttatccgccg acggtgaata cttactggcg
     1801 acgtcgtggc agcacatatt ttgtatcaaa agttggtgag cgttatcgcc gtgatgtgac
     1861 gctaattgtt cgccagcagc ggctgaaatt aaacctgtcc ggaaggctgg cgataaagat
     1921 tattgcag
//
LOCUS       DAJVXP010000273         1899 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVXP010000273 DAJVXP010000000
VERSION     DAJVXP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.279"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..140)
                     /locus_tag="OQS10_004200"
     CDS             complement(<1..140)
                     /locus_tag="OQS10_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCS2295058.1"
                     /translation="MKKFFRHFFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQKW
                     RET"
     gene            complement(193..789)
                     /locus_tag="OQS10_004201"
     CDS             complement(193..789)
                     /locus_tag="OQS10_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2295059.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(816..1418)
                     /gene="yadL"
                     /locus_tag="OQS10_004202"
     CDS             complement(816..1418)
                     /gene="yadL"
                     /locus_tag="OQS10_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCS2295060.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(1433..>1899)
                     /locus_tag="OQS10_004203"
     CDS             complement(1433..>1899)
                     /locus_tag="OQS10_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2295061.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          506 a          422 c          390 g          581 t
ORIGIN      
        1 gtttctctcc atttttgatc tgacggacca acgccgtaac tattcccaac aatatatcct
       61 acgttatcat cagtgccagc actcgcggta taacaactca agcacagaat taaaaaaaaa
      121 tgtctgaaga atttcttcat tatcactcca ccatctactg ctgtaacagt cgcagttatt
      181 tgttttgatc tattattcgt agtacacatc aactaatata tgactcatta cttggccaga
      241 tatgacatcc agcgcgggat cgattttttg cattctggca taaaatttcc atgaagaatt
      301 atgcagggca gaagtcatta ctggatacac ggaacgagaa tttcctgctg aactaataac
      361 attgaatata ttggtcgggt cctgaatgga aaagagaacg acaccgacat ttttagcccc
      421 ggttgcgact tcattggcaa atatttgcct tgaacctgca gccatgacac cgctaagggc
      481 tctaaaatca atatgtaact gattaagcac atcgccagtt ccctgtaatg cacagtctga
      541 tactgtgaca gtgaactctt ttccacctgg ttgatagctt tcaggagtaa cattatctga
      601 aaaatatccc accccaacga cgccaagatc aatattaccc tggttactga ttccgctttt
      661 gcaagtgctg ttttttacat tggcaacaag gtcaatatcc tgcccagcga tggctggata
      721 ggtaatggga aactgtagtg acagaaacag aattattctc ttcatcagct tacgttgagt
      781 gggatgcata tatagttcct ggatctgtca gtctgttagt tataggttac tgtaaatgtg
      841 gcaggtgcag aaaatttacc cgcagtgaca ttgcttacgg ttttattttt ttcgacgacc
      901 agtcgagcac tcatcggata ataaacaacc gcgttggctg cattggagat agtgacctct
      961 tgagatgctg gtgtcacaca actaaattgt gttgcgctcc ctgttgccgg agtttcggtg
     1021 ctccagactt caacggcgac ggcacttgct ttatctgcgg ctgtggatcc atttgcgagc
     1081 cccgcaccgt cattagcagt accttcgcat gtggctcgct tggttaattt tatttgtgct
     1141 tttttattgg ggatacccgc acagtctttg aatttgagtt tgaatgtttt aactttggtc
     1201 ttggcattga tttcagaaat atatacatcg ccaaagtcaa caacagaaat gtcctgatca
     1261 gagtcattta ctaatgtagc ggtacaagta cctatttcta cggtagtaat cacggtcagc
     1321 ccaatgctgt ctgttgccgc ataagagggt acgctgaaca atgaggcagc caaaacgaca
     1381 ctgctggata atccataacg taaattttta aatgtcatct taattatcct tcttattgat
     1441 agatgaaatt aaacgttgcc agtgcacgaa aattccccgg tacgccttgc cctgcatctg
     1501 tttcacgtag cgcaacggtc atttcaagac ccttatcggg ctgcatctcg tctgtgctcc
     1561 agcgtatctt ttccgcactg ttaggtttaa ggaaagtggc atcatcagta gtccgttttt
     1621 tgaaacccat accgatatta cttgtcgtcg aagatgaatc accagactgc ggtataataa
     1681 gcttaggtga gcttgatgat gcatttccgg tcagagtggt atcaatccaa ctgatgccac
     1741 tgctgcaccc actagcaacc agtttaaaat cagcctgaga ttctgtcgtt ttattcgcga
     1801 tcttatccag acccatctta gggattctca atgtgtagtt attattgcca tcattcgtga
     1861 cgttattacc agtaagtgtg atgttacagg ttgtcgctt
//
LOCUS       DAJVXP010000274         1898 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVXP010000274 DAJVXP010000000
VERSION     DAJVXP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.280"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..145
                     /locus_tag="OQS10_004204"
     CDS             <1..145
                     /locus_tag="OQS10_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS2295062.1"
                     /translation="LLLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGD
                     NGQ"
     gene            167..1591
                     /gene="patD"
                     /locus_tag="OQS10_004205"
     CDS             167..1591
                     /gene="patD"
                     /locus_tag="OQS10_004205"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCS2295063.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            1801..>1898
                     /locus_tag="OQS10_004206"
     CDS             1801..>1898
                     /locus_tag="OQS10_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCS2295064.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT          483 a          392 c          550 g          473 t
ORIGIN      
        1 gttactgttg tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg taactaacgt
       61 ggtggcactg ctggttatgt tggtaacaac cttgccgatc ctgggggcct ggtggctaac
      121 ccgcgaaggc gacaatggtc aataaccact gatacaggaa tatgctatgc aacataagtt
      181 actgattaac ggagaactgg ttagcggcga aggggaaaaa cagcctgtct ataatccggc
      241 aacgggggac gttttactgg aaattgccga ggcatccgca gagcaggtcg atgctgctgt
      301 gcgcgcggca gatgcagcat ttgccgaatg ggggcaaacc acgccgaaag tgcgtgcgga
      361 atgtctgctg aaactggctg atgttatcga agaaaatggt caggtttttg ccgaactgga
      421 gtcccgtaat tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc cggcgattgt
      481 cgatgttttt cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg cggcaggtga
      541 atatcttgaa ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg tggcttctat
      601 cgcaccgtgg aattatccgc tgatgatggc cgcgtggaaa cttgctccgg cgctggcggc
      661 agggaactgc gtagtgctta aaccatcaga aattaccccg ctgaccgcgt tgaagttggc
      721 agagctggcg aaagatatct tcccggcagg cgtgattaac atactgtttg gcagaggcaa
      781 aacggtgggt gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc tgacgggctc
      841 tatcgccacc ggcgagcaca tcatcagcca tgccgcgtcg tccattaagc gtactcatat
      901 ggaacttggt ggcaaagcgc cagtgattgt ttttgatgat gcggatattg aagcagtggt
      961 cgaaggtgta cgtacatttg gctattacaa tgctggacag gattgtactg cggcttgtcg
     1021 gatctacgcg caaaaaggca tttacgatac gctggtggaa aaactgggtg ctgcggtggc
     1081 aacgttaaaa tctggtgcgc cggatgacga gtctacggag cttggacctt taagctcgct
     1141 ggcgcatctc gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag ggcacatcaa
     1201 agtgatcact ggcggtgaaa agcgcaaggg taatggctat tactatgcgc cgacgctgct
     1261 ggctggcgca ttacaggacg atgccatcgt gcaaaaagag gtatttggtc cagtagtgag
     1321 tgttacgccc ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca gccagtacgg
     1381 acttgcatct tcggtatgga cgaaagatgt gggcagggcg catcgcgtca gcgcacggct
     1441 gcaatatggt tgtacctggg tcaataccca tttcatgctg gtaagtgaaa tgccgcacgg
     1501 tgggcagaaa ctttctggtt acggcaagga tatgtcactt tatgggctgg aggattacac
     1561 cgtcgtccgc cacgtcatgg ttaaacatta agttagatgt tagtggctgt tataggacgg
     1621 ccactatcgt tttaggttct gagtttaatg gcggttaagt attagaatga ttacattaat
     1681 aactagcgta aaaattaggc taaatgtttg atggcgattt ttcatcctgt cacagttgat
     1741 tagcataagg ctttagagta tcgttattaa actaataatt tataagtaga agtattaaca
     1801 atgaaagttc aattgtacaa attcacggaa gataaaaata aaacccttac cttcagatgg
     1861 acgaaaaaac actttgagtt ttgtatggat aataaaat
//
LOCUS       DAJVXP010000275         1870 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVXP010000275 DAJVXP010000000
VERSION     DAJVXP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.281"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..526
                     /locus_tag="OQS10_004207"
     CDS             <1..526
                     /locus_tag="OQS10_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HCS2295065.1"
                     /translation="ANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSM
                     GTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLPGIFNASLVDEVLD
                     IHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVVAIICDRGDRYLST
                     GVFGEEHFSQGAGI"
     gene            complement(529..897)
                     /locus_tag="OQS10_004208"
     CDS             complement(529..897)
                     /locus_tag="OQS10_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS2295066.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            1002..1853
                     /gene="pdxK"
                     /locus_tag="OQS10_004209"
                     /gene_synonym="thiJ"
     CDS             1002..1853
                     /gene="pdxK"
                     /locus_tag="OQS10_004209"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS2295067.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT          451 a          438 c          526 g          455 t
ORIGIN      
        1 ggcgaatcgt ggcgaaggaa agctgctcga tcagttcaat aatcccgata acccttacgc
       61 ccattacacc actaccgggc cggaaatctg gcagcaaacc ggcgggcgca ttacccattt
      121 tgtctccagc atgggaacaa ccggcactat caccggcgtt tcacgcttta tgcgcgaaca
      181 atccaaacca gtaacgattg ttggcctgca gccggaagaa ggcagcagta ttcccggcat
      241 tcgccgctgg cctgcggaat atctgccggg gattttcaac gcttctctgg tggatgaggt
      301 gctggatatt catcagcgcg atgcagaaaa caccatgcgc gaactggcag tgcgggaagg
      361 aatattctgt ggcgtcagct ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc
      421 taaccctggt gcggtggtgg tagcgatcat ctgcgatcgt ggcgatcgct acctttccac
      481 cggggtgttt ggggaagagc attttagtca gggggcgggg atttaagatt attgcgcagg
      541 caccgtttta tccgtctgcg caattaaggc atgtaaaaaa ggctgtgcgt tctcatcgct
      601 cttaccgggg atcttcacaa tatacggctt cccggtaatc gatgacgacg aagcaacctt
      661 atcaataaac tgctcggcag tgtcaatgcg gttacgggta ttgccgagct tcagacgcag
      721 atgagaaacc gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt
      781 tttctgtgct aatccctcca gcatggcgtt gatgcgggct tcttcatgaa cagttaactt
      841 cgcgtaagcg gggagtgtca ttagtagtgt aatgaccaga caaatgattt tcttcattga
      901 attaccttat ccttgttttg tgatagccga tttgactgcc agaatcaggt aaagtttcgg
      961 ttggaatacg atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt
     1021 ttaacgataa gagtagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtagtgt
     1081 acggcagcgt aggcaacagc attgcggtgc ctgctataaa acagaacggc ttgaatgtct
     1141 ttgccgtgcc gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg
     1201 cgattccgga cgaatggttt agcggctatt tgcgtgcgct tcaggagcgt gatgcgctgc
     1261 gccaacttcg tgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg
     1321 ccgagtggct gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg
     1381 tgattggcga tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac
     1441 aatatttact gccgctggcg caaggaatta cccccaatat ctttgagctg gaaatcctga
     1501 ccggtaaaaa ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag
     1561 acacattaaa atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc
     1621 aggtggtggt ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg
     1681 acctgaaagg gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga
     1741 aggcgttaaa cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctata
     1801 cccagcagca tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg
     1861 cgccgatggg
//
LOCUS       DAJVXP010000276         1843 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVXP010000276 DAJVXP010000000
VERSION     DAJVXP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.282"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1008
                     /gene="istA"
                     /locus_tag="OQS10_004210"
     CDS             <1..1008
                     /gene="istA"
                     /locus_tag="OQS10_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCS2295068.1"
                     /translation="KPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPK
                     RKMRPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPK
                     QDAEHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLP
                     RACRPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQF
                     KETPEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVS
                     IRISLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEEL
                     L"
     gene            1008..1805
                     /gene="istB"
                     /locus_tag="OQS10_004211"
     CDS             1008..1805
                     /gene="istB"
                     /locus_tag="OQS10_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCS2295069.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          482 a          439 c          534 g          388 t
ORIGIN      
        1 aaaccgttta tggattacat cgacatgcgc ctggcagaga atgtctggaa tagcgaggtt
       61 atcttcgcgg agattaaggc aatgggttat acgggcggac gttccatgct gcgttactac
      121 atccagccca aacgtaaaat gcgtccgtca aaaagaacag ttcgcttcga aactcagcct
      181 ggataccagc tccagcatga ctggggcgaa gttgaggtgg aggttgccgg gcaacggtgc
      241 aaagttaact ttgcggttaa tacgctgggg ttctcccgca gcttccatgt cttcgccgca
      301 ccaaaacagg atgctgagca tacctacgaa tcactggttc gcgccttccg ctacttcggt
      361 ggttgtgtga aaacggtgct ggttgataac cagaaggctg cggtgctgaa gaataacaac
      421 gggaaagtcg tgttcaactc cggattcctg ttgctggctg accactataa cttcctgcca
      481 cgggcatgcc gtccacgcag ggccagaaca aaaggtaagg ttgagcggat ggtgaaatac
      541 ctcaaggaga acttcttcgt ccggtaccgc aggttcgaca gcttcactca tgtcaatcaa
      601 caactggagc aatggatagc cgatgtggct gacaaacggg agcttcgcca gttcaaagaa
      661 acgccggaac agcgcttcgc gctggagcag gaacatctgc agccgttacc ggatacagac
      721 ttcgatacca gttacttcga tatccgccat gtgtcctggg acagctatat cgaggttggt
      781 ggtaatcgtt acagcgttcc cgaagcgctg tgtggtcagc cggtatcgat acgaatatcg
      841 ctggatgacg agttgcggat ctacagtaat gagaaactgg tggcctcaca tcgcctctgt
      901 tcagcatcgt ctggctggca gacagtgccg gagcatcacg ccccgctctg gcagcaggtc
      961 agtcaggtgg aacatcgacc actgagtgcc tatgaggagc tgttgtgatg catgaactgg
     1021 aagtcctgct gagtcgcctg aaaatggagc atctgagtta tcacgttgaa agcctgctgg
     1081 aacaggcagc taaaaaagag ctgaactacc gggagttcct gtgcatggcg ctacagcagg
     1141 aatggaacgg caggcatcag cgcggtatgg agtccaggct gaagcaggct cgtctgccgt
     1201 gggtcaaaac gctggagcag ttcgacttta ccttccagcc gggcatcgac cgtaaggttg
     1261 tccgggaact ggctggtctg gcgttcgtgg agcgcagcga aaacgtgatc ctgctgggcc
     1321 ctcctggtgt cggaaaaact catctggcca tagctcttgg cgtgaaagcg gtggatgcgg
     1381 gacatcgggt actgtttatg ccactggaca gactgatcgc gacactgatg aaagcgaaac
     1441 aggaaaaccg gctggagcgt cagctacagc aactgagtta tgcccgggtg ttgatcctgg
     1501 atgaaatagg ctatctgccg atgaacagag aggaagccag tctgttcttc cggctactga
     1561 accgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaagggttc gcagactggg
     1621 gagaaatgtt cggagatcac gtactggcaa cagcgatcct ggatcggctg ctacatcact
     1681 caaccacgct gaatatcaaa ggagagagtt accggttaaa agagaaacgt aaagctggag
     1741 tgctgaccaa aaacacaacg ccaatcagtg atgatgaaat ggtggaaagc ggacagcatc
     1801 agtaacgaaa gtatttagcg ggcatgaaaa tggcaaataa cgg
//
LOCUS       DAJVXP010000277         1812 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVXP010000277 DAJVXP010000000
VERSION     DAJVXP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.283"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(2..853)
                     /gene="hchA"
                     /locus_tag="OQS10_004212"
     CDS             complement(2..853)
                     /gene="hchA"
                     /locus_tag="OQS10_004212"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS2295070.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1109..1304
                     /locus_tag="OQS10_004213"
                     /pseudo
     CDS             1109..1304
                     /locus_tag="OQS10_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1444..>1812)
                     /locus_tag="OQS10_004214"
     CDS             complement(1444..>1812)
                     /locus_tag="OQS10_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS2295071.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          476 a          369 c          442 g          525 t
ORIGIN      
        1 attaatccgc ataagctgcc agcatttcct gcgccgccag tttacccaac gcattcgctg
       61 cgaaaggact gtcgccggtg agaactttac ggtccttatg tactcgcccg gtgatgtcgt
      121 cattaataat gttcatgccc attttcttca gctcttcgcc gaagtaccag gtgagatgac
      181 ccggcatata gcctatctct ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg
      241 aataaccatt cagtgggtta tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc
      301 aaagggagat aacaaaacgg tcatttttga ttgcccactg caaagcagca gctacgtcct
      361 ggctttctgg taaaccaata agtgcgccat gaccaccagg aacaaagatt gctgcatatt
      421 cgctatcagc gttgaggctg gcgacaacat ccgcgagttt cttcggattg cggaacaacg
      481 atttatgctg ctcaaagaat ggcattactt tttcatcttt gtgcggcata gcccagtatt
      541 caaacttggt catcagaccg gaaatggtcg ccacttcgaa ttcgaaacca gcagcatgga
      601 gatgatacaa cggcagcaac gtttcaatcg gatggttacc ggtcgagaac agttttccgt
      661 tatcggttgg caaataacgt tcgtccgcgg cgatcaccag aattttatgc ttaccgcgat
      721 acggttttgg atagtccacg ccatcaagat cagagacggg actggtatat tggctaagcg
      781 aatattctga agggaagaat gcattatctt cagcaatatc gacctgcgga tttttacttg
      841 tttgaacagt catagtgtat tccttattgt tgcttagtta gggtagtcac tatatttgcg
      901 actgagctgg tcgctatttt taaccagtct ggaaaattga gtcaatatat cgaaagcggt
      961 agtcggcggg tgtaagtatg taatgatgat gctgcactgg tgttgaatat tattcacgcc
     1021 aaattttcct tattgagcaa cgtcgtcgca agtggcctca atgattttag gccattatca
     1081 aaggtacggg gcggcgggga gagatttagt gctgtgaaat attgtggtac tgatgaatat
     1141 ttatatcgcg gattggtaga gaaaagatta tttcatgcac tatttttgca aatatttcta
     1201 tatgggaagc gtgtttgtta cggattaatt cataaagaaa tgcatttggg tgttggtttg
     1261 aagagtcgct ggttgatgtt attgtgccag gaggggcagg gtaaggtgct gtatcgatat
     1321 gcattggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa gcctgaattc
     1381 gatgagggac gaactcaggc tagtgtttag ttttacagta acacgccgca gcacgttggc
     1441 tgattagaac tggtagacta agcccacacc aacaacgtca tctgttgcga tgccattgtt
     1501 gtcatagaaa tcgtcatctt tatccaacaa gtttatttta tagtcaacat atgttgagaa
     1561 gttcttattg aaatagtagg tcgctccaat ttcaacatag ttgaccagat cctgatcaac
     1621 atactcctga tgaccattgt tgttatagtc gttaccgccg aggtctttgc cttttgaata
     1681 aacatatgca agagatgggc gcagaccaaa atcgaactga tactgtgcta cagcttcaaa
     1741 gttctgggtt ttatttgcga tacccacatt accatatggt gtcatattgc gggtttcagc
     1801 atacatcatt gc
//
LOCUS       DAJVXP010000278         1792 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVXP010000278 DAJVXP010000000
VERSION     DAJVXP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.284"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..652
                     /locus_tag="OQS10_004215"
     CDS             <1..652
                     /locus_tag="OQS10_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295072.1"
                     /translation="QKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQ
                     IQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSS
                     QVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLT
                     KDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            728..>1792
                     /locus_tag="OQS10_004216"
     CDS             728..>1792
                     /locus_tag="OQS10_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2295073.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALW
                     QVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGD
                     VAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPDTLP
                     AGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQVRGF
                     TYDAEHAGRMVAHHYAGRPES"
BASE COUNT          389 a          467 c          595 g          341 t
ORIGIN      
        1 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga taaacaacca
       61 tgccgaaaaa ataggtaaca accaggcgat caccgttacc aataaccaga tccagaacat
      121 tggcgttaat cagatacaga cggttggtgt caacctggtg gaaacggtgg gcagtaacca
      181 gattatcaaa gtgggatcaa accaggttga aaaggtgggg atcattcgtg cgctgacggt
      241 gggtgtggct taccagacga cggtaggcgg cattatgaat acctcggtgg cgttgttgca
      301 gtcctcacag gtagggctgc ataaatcact gatggtggga atgggctaca gcgtcaatgt
      361 agggaataac gtcaccttct cggtgggcaa gacgatgaag gaaaacaccg gacaaacagc
      421 agtttattct gccggtgagc atcttgaact ctgctgtggt aaggcaaggc tggtgctgac
      481 gaaggacgga agcatatttc tcaacggtac gcacattcat ctggaagggg agtcggatgt
      541 gaacggtgat gcgccagtga ttaactggaa ctgtggtgcc acacaacctg taccggatgc
      601 gcctgtgccg aaagatttac cccccggaat gccggatatg cggcaatttt gagtcaggtg
      661 caaaaaatta aaacggaact ggtgcagtga ccagatgaac catattaaaa gcagaaggaa
      721 ttgattcatg ggcggcggaa aaccggcggc gcggcagggc gacatgaccc gcaagggact
      781 ggatattgtg cagggttcag cgggtgtgct gataggtgcg ccgacgggag tggcctgttc
      841 ggtgtgtccg ggagggatta cctatgctaa cccggtgaac ccggtgctgg gtgcgaaggt
      901 gcttccgggc gagacggacc ttgcgctgcc ctgcccgctg ccgtttattc ttttccgcgc
      961 ctacagcagc taccggacca gaacacccgc gccggtgggg gtgtttggtc ccggctggaa
     1021 agcgcccttc gatatccgct tacagatacg cgatgaaggc ctgatactca acgacagcgg
     1081 cggcaggagc attcactttg agccgctgtt tcccggcgag ataagctaca gccgcagcga
     1141 gtcactctgg ctggcgcggg gcggagtggc ggcgcagcac agttcgcagc cgctaagcgc
     1201 gctctggcag gtgctgccgg aagatgttcg cctgagtccg catgtgtacc tggcgacaaa
     1261 cagccttcag gggccgtggt ggatactcag ctggccggag ccgcccgcat accgggtact
     1321 gacggtcgtg gtggatggct tcgggcgctc actgactttc caccgggcgg cggagggtga
     1381 cgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc cgccgttttc acatggcgct
     1441 gagcacgcag gcgcagcggg cggaagcgtc ccgtaaacag cgcgcctctt ccttatcttc
     1501 cccggccagt ccccgttctg tttcctcttc tcaggttttc cccgacacgc tgcccgccgg
     1561 tacagagtac ggtgccgata acggtatccg gctggaggcg gtatggctga cacacgaccc
     1621 ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc tacacgtaca cggccggtgg
     1681 tgaactgcgg gcggtgtatg accgcagcgg gacgcaggtt cgcgggttta cttatgatgc
     1741 ggagcacgcc gggcggatgg tggcgcacca ttatgcgggg aggccggaga gc
//
LOCUS       DAJVXP010000279         1733 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVXP010000279 DAJVXP010000000
VERSION     DAJVXP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.285"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1138
                     /locus_tag="OQS10_004217"
     CDS             <1..1138
                     /locus_tag="OQS10_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2295074.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="OQS10_004218"
     CDS             1150..1599
                     /locus_tag="OQS10_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295075.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          523 a          288 c          497 g          425 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cag
//
LOCUS       DAJVXP010000280         1733 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVXP010000280 DAJVXP010000000
VERSION     DAJVXP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.286"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(28..1086)
                     /locus_tag="OQS10_004219"
     CDS             complement(28..1086)
                     /locus_tag="OQS10_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCS2295076.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1194..1434)
                     /locus_tag="OQS10_004220"
                     /pseudo
     CDS             complement(1194..1434)
                     /locus_tag="OQS10_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1659..>1733)
                     /locus_tag="OQS10_004221"
     CDS             complement(1659..>1733)
                     /locus_tag="OQS10_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2295077.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          414 a          508 c          379 g          432 t
ORIGIN      
        1 taacgacgcg taattaattc ttcaatatca tccgttctgg ctgactaaat cctgtacttc
       61 ccgaaccgtc tgctcaaaac gttcagtctc cagctcaacg ccaattgcac gacgcccgag
      121 cgccagtgcc gctttcactg tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc
      181 cggacgactg ctagcgctga ttatctgctg cagcatttct gccggttttt cgcacggatg
      241 tttcccggga tagaactgca ccggtttatg cgtccacaca tcggtatacg gcacctgcac
      301 cgtcacgcca aaataccgcc gcagatgttt atattcactc agcagctccg catactgccg
      361 gttcagtgag gtatacgtat ccaccagctg gtggtggagc ttttccagtt caccacgccg
      421 atgcttctct tctgccaccc gggcaaacag cgcctgtaat ttcagataat cgctttcatt
      481 cggcaactgc cactgaccgg cactgaacca gtgcgacacc atgtttttct ttcctgtggc
      541 atctgcaatc tgttttgccg ttatccccag ggcagctcgc gcatcacgaa agtaagaaat
      601 cagcggggcc atcacatgct gtttcagtgc actgcccttc gccgcatacc cggcatcttt
      661 cggacgatac ggcccctgat aatgttccgc gaacagaatg cgctctgtgg cggggaaata
      721 cgcccgcagg ctttccttgt tgcatccgtt ccagcgtccg gacggcttcg cccagataat
      781 atggttcagc acactgaagc gttcacgcat catgatttcg atatcagatg ccaggcgatg
      841 accacagaac aggtaaagac ttccggcagg tttcagcacc cgccagaact gcgccagaca
      901 ctggtccagc cacttcaggt aatcatcgtc gcccttccac tggttatccc agccctcagg
      961 cttcacttta aagtacggcg ggtccgtgac tatcaggtca acagaatttt cgggtaacga
     1021 ccggataaat tccaggcagt cggcgttgat taactcacaa ctggatattt ttacagtatt
     1081 aagcatggat cattaagcct gtctctgata ggctcattct gcttttgcgc aaagcagtgg
     1141 gcctgaggtt tgcttgtgaa cccaacgcat gagcagatgg ctggtgggtg cccctaacac
     1201 ccaccagccg cccatttacc acaaataaaa aagccttcac tgcggaaggc gtctgtaaca
     1261 accgaactga tagtctgcca gacccgccat aaccagctgg gtcagtatta actggcagcg
     1321 ttcgcgtgaa aggtaagtat tctgcgctat ctccccgact gtcgccggtt cggtaacgct
     1381 taattcatta aacaccactc tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt
     1441 tccttttccg gttaacgtga cacaccaata actcttgtcg aaaaagccag caagctgaaa
     1501 gacaggtatt caccgccacc agcgcgttta ctgtactgac gcgatttcag tcataaaaaa
     1561 cccgctcggc ggcgggttgt agaaactctt ctaacgtcag gcataaaacg cccatcgtta
     1621 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     1681 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAJVXP010000281         1717 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVXP010000281 DAJVXP010000000
VERSION     DAJVXP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.287"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(48..251)
                     /gene="tatE"
                     /locus_tag="OQS10_004222"
     CDS             complement(48..251)
                     /gene="tatE"
                     /locus_tag="OQS10_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS2295078.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(380..1168)
                     /locus_tag="OQS10_004223"
     CDS             complement(380..1168)
                     /locus_tag="OQS10_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS2295079.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1261..1644
                     /gene="crcB"
                     /locus_tag="OQS10_004224"
     CDS             1261..1644
                     /gene="crcB"
                     /locus_tag="OQS10_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS2295080.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
BASE COUNT          423 a          465 c          404 g          425 t
ORIGIN      
        1 tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca ctctttatga
       61 gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc atcgtcatca
      121 ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag cgtacgtaac
      181 ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt ggtaatacta
      241 atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc ttaaaaacca
      301 gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca aggtgaatca
      361 agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg ttgtttaaga
      421 cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc ataatgagtg
      481 caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc cggctttgcc
      541 cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta tccagcgcac
      601 gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc caggcggcag
      661 gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct ggaaagcgta
      721 agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac ggagcgattt
      781 cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag agatgcaatt
      841 tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc catgcgcgcc
      901 ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt ttactttctc
      961 gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc gatagatctg
     1021 catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat acgtcgtttt
     1081 ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac acagatgtaa
     1141 cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt aaaattcgcc
     1201 aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca ggattttgca
     1261 gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt ggcgagatgg
     1321 ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac gctggcagca
     1381 aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag gatgacgaac
     1441 attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct aacaaccttc
     1501 tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg ctgggcgtta
     1561 ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc tttctggctg
     1621 ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag cgggttttga
     1681 attcttgctg acgtatctta cagagcgatt acgtttg
//
LOCUS       DAJVXP010000282         1700 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVXP010000282 DAJVXP010000000
VERSION     DAJVXP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.288"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>1700
                     /locus_tag="OQS10_004225"
     rRNA            <1..>1700
                     /locus_tag="OQS10_004225"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          425 a          375 c          542 g          358 t
ORIGIN      
        1 ggagcgttct gtaagcctgc gaaggtgtgc tgtgaggcat gctggaggta tcagaagtgc
       61 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
      121 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      181 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      241 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      301 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      361 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      421 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      481 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      541 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      601 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      661 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      721 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      781 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      841 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      901 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      961 gcatggagcc ggccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
     1021 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     1081 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     1141 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     1201 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1261 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1321 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1381 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     1441 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     1501 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     1561 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     1621 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     1681 aaccggtact aatgaaccgt
//
LOCUS       DAJVXP010000283         1681 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVXP010000283 DAJVXP010000000
VERSION     DAJVXP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.289"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(423..680)
                     /locus_tag="OQS10_004226"
     CDS             complement(423..680)
                     /locus_tag="OQS10_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295081.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OQS10_004227"
     CDS             complement(677..1174)
                     /locus_tag="OQS10_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS2295082.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..>1681)
                     /locus_tag="OQS10_004228"
     CDS             complement(1376..>1681)
                     /locus_tag="OQS10_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS2295083.1"
                     /translation="ADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQV
                     AATCAKSTTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          379 a          440 c          384 g          478 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 c
//
LOCUS       DAJVXP010000284         1676 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVXP010000284 DAJVXP010000000
VERSION     DAJVXP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.290"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..889)
                     /locus_tag="OQS10_004229"
     CDS             complement(<1..889)
                     /locus_tag="OQS10_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2295084.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="OQS10_004230"
     CDS             complement(989..1345)
                     /locus_tag="OQS10_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS2295085.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="OQS10_004231"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQS10_004231"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAJVXP010000285         1667 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVXP010000285 DAJVXP010000000
VERSION     DAJVXP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.291"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(183..604)
                     /locus_tag="OQS10_004232"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="OQS10_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(588..815)
                     /locus_tag="OQS10_004233"
     CDS             complement(588..815)
                     /locus_tag="OQS10_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2295086.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="OQS10_004234"
     CDS             892..1299
                     /locus_tag="OQS10_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS2295087.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="OQS10_004235"
     CDS             1503..1658
                     /locus_tag="OQS10_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS2295088.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          469 a          436 c          345 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacgca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAJVXP010000286         1636 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVXP010000286 DAJVXP010000000
VERSION     DAJVXP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.292"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1120..1455)
                     /locus_tag="OQS10_004236"
     CDS             complement(1120..1455)
                     /locus_tag="OQS10_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCS2295089.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
BASE COUNT          581 a          310 c          246 g          499 t
ORIGIN      
        1 agtcacataa accaccgatt gagtgacagt tactgaaaca actatttcga cacaaaatct
       61 cgtctattca acatcatgca aatttgtcag tgactgaatt tagtgacaga accctctgga
      121 attctgccgt gtaaccactt atatgcagac ctgccccgtt gctctgggtt ttcaggctgc
      181 gatgagtgat gagcgatgat ttccatacag atgatatatc gactggaatt gattgcctgc
      241 aattagattt agttttattg tgtggatcgt tttagttagt ggggatgaat ttccgataaa
      301 ttaaccaatg gaaaggagat gatatgaata aaaagataac tggattatta ataattagtc
      361 tggcgtcaat tttcttaatt gattgcaccc agtgccatta acatctatct acacaaaaat
      421 agaggaaaac aattttgata tatcaaataa ttacaagcag ctccattctt tttattgttt
      481 gggggattac atctataata attagaacaa agaattattt ttctaagaag aaaacagacc
      541 ttaatataag cttttttata gccttcccta tttctataac aatcctttta ttttgccgag
      601 gtatattata aatataacat aatacatcta tatggggtgt acatatcccc acataaaaac
      661 acatactctc attaacacgg gaagaaacta tgttttccaa ttctcactac atgtacataa
      721 tcgttcttgt actatggttt agtattctca agtacttaca gagaaagaat agcatattac
      781 atcctttatt ttcactaatc ctgacaccac taaccatttt aatcattaga cttatgatag
      841 atttaacata caaccaataa attataaaca aaataaaaat agggaacatt atatgcttta
      901 taaaattttc acattaaaca caaagataaa aacaactatc attacaatat gtgcattatc
      961 atataatctt acagcgcaag cttccccgtc atacagacct cgtgatcgag ttaataagac
     1021 tatgatagaa ctacaaacta acggagtaga acggcaactt ttagataatt cttgtggcat
     1081 aagttctggg gtatttaaat aaaatataag acaggctgtc tatcttacag acagcctttt
     1141 tataataaca gaaagaatat atatcatact taacggaaga gataatatga aaacagtacc
     1201 cgtataaaca gcaagcaatc ccatttttcc aggtgtatca gcaaaaaaaa cgctgttcca
     1261 gaaatcaggc cgggtaaatt tcagagctgt atcttcaata taccattttg caaccggata
     1321 caaaaccatt ccgcagagag aaatacacca gaacagtaat ctgtatttaa aatcataatc
     1381 ccatgacata tacagcatat atcctcccgt cacccatccc caccacatat tattaaaata
     1441 atattttctg ttcatctgcc agttttcctt tcttttgcac ttttgtcagt gtactgatgc
     1501 atgacaacat aaccacagca tgataataat aatcaataac aataagctgt gtcacgttta
     1561 catcatggaa cggaggtgct cctgtgactt ttgagcagaa aaaagcccgg gccattgccc
     1621 tgatggacag caaaaa
//
LOCUS       DAJVXP010000287         1627 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVXP010000287 DAJVXP010000000
VERSION     DAJVXP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.293"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="OQS10_004237"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="OQS10_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCS2295090.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..>1627
                     /gene="hipA"
                     /locus_tag="OQS10_004238"
     CDS             641..>1627
                     /gene="hipA"
                     /locus_tag="OQS10_004238"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCS2295091.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTP"
BASE COUNT          453 a          358 c          428 g          388 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgcca
//
LOCUS       DAJVXP010000288         1626 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVXP010000288 DAJVXP010000000
VERSION     DAJVXP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.294"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            89..1624
                     /gene="istA"
                     /locus_tag="OQS10_004239"
     CDS             89..1624
                     /gene="istA"
                     /locus_tag="OQS10_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCS2295092.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRDNVELPLSRLGEQHPAYASEHENLRGPG
                     YYH"
BASE COUNT          411 a          459 c          411 g          345 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttgaatcc atgctgaaag ccaaacccca
     1381 tccggagctg gcttaccgtg cagtcctggg tctgctggca cttcagaaaa aatacggccc
     1441 ggaacggctg gaaaaggcct gttacgttgc cctgcactat aacgccccgg acagacgctt
     1501 tatcgacaat ctgctgcgtc atcatcggga taacgttgag cttccgttat cccgcctggg
     1561 cgaacaacat ccggcatatg cttcagagca tgaaaacctg cgtggtcctg gctactacca
     1621 ctgaca
//
LOCUS       DAJVXP010000289         1570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVXP010000289 DAJVXP010000000
VERSION     DAJVXP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.295"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..268
                     /locus_tag="OQS10_004240"
     CDS             <1..268
                     /locus_tag="OQS10_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS2295093.1"
                     /translation="LGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIR
                     RKDAVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(309..1304)
                     /gene="pgl"
                     /locus_tag="OQS10_004241"
     CDS             complement(309..1304)
                     /gene="pgl"
                     /locus_tag="OQS10_004241"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS2295094.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          402 a          426 c          371 g          371 t
ORIGIN      
        1 attaggactg gtagaggcgg gactggggat ttcggcgctt cctggtctgg caatgcccca
       61 tgcgccctat tcttcgatta ttggtattcc actggtggaa cccgttatac gtcggacatt
      121 ggggattatt cgccgcaaag atgccgtgct ttctccggca gcagaacgct tttttgcctt
      181 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
      241 gagacatgcg ttacaggaga taggttaata caccggcgca ggagagactg cgccgggtaa
      301 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
      361 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
      421 ttttgcccgg cgacaatcag atacttgccg ctgtgatcaa cattgaagcc gcgcggctgg
      481 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
      541 acggtaatca gactggcggt acggtcgcag gcgtataaat gacgaccatc cggggtgata
      601 tgaatatcag ccgcccaaca ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
      661 acacattcaa tattgccgtg tggatctttc agttcccaga catccactga gctgtttaac
      721 tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
      781 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
      841 gtaaacaggc aaatgcgatc ctgctttaat gccggaaccc acagcgtacg gttgtccggt
      901 gagatattgg cggaatggca accgtccagc ccctcgacca catcgacgac gcccactggc
      961 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1021 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcgc agactctgcg
     1081 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1141 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     1201 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     1261 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     1321 tgataaatcc cttatctttc atacacataa caacacttac tggataaaaa acagacaaca
     1381 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     1441 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     1501 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     1561 acactatgcc
//
LOCUS       DAJVXP010000290         1557 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVXP010000290 DAJVXP010000000
VERSION     DAJVXP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.296"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            271..1386
                     /locus_tag="OQS10_004242"
                     /pseudo
     CDS             271..1386
                     /locus_tag="OQS10_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          614 a          280 c          221 g          442 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc ttatcaaatc attatcagaa tataatacca taatattgat
     1501 tattagcact ttataattat tgaatgaaaa tagattttat gtaattttaa aacaaag
//
LOCUS       DAJVXP010000291         1524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVXP010000291 DAJVXP010000000
VERSION     DAJVXP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.297"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(431..562)
                     /locus_tag="OQS10_004243"
     CDS             complement(431..562)
                     /locus_tag="OQS10_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCS2295095.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1062..1138)
                     /locus_tag="OQS10_004244"
     tRNA            complement(1062..1138)
                     /locus_tag="OQS10_004244"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1102..1104),aa:Arg,seq:tct)
     gene            complement(1239..1314)
                     /locus_tag="OQS10_004245"
     tRNA            complement(1239..1314)
                     /locus_tag="OQS10_004245"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1279..1281),aa:Ile,seq:cat)
     gene            complement(1417..>1524)
                     /locus_tag="OQS10_004246"
     CDS             complement(1417..>1524)
                     /locus_tag="OQS10_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2295096.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          439 a          412 c          300 g          373 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaccagac aacagtcaac cgcctgaatt gtgaagtatt taaaaatttc tcccgctaac
      421 tgatacccgg ctaacagtct ggcgttttct ttttcagcaa cgggaaagca acaaccacca
      481 cacccgccac cagcacaccg tcagccagca ctgacattat ccggctgctg caatgccatt
      541 cacaaaaaca gtaagcaatc actttttacc gtaacaggtg ataatccaga tatgtatcta
      601 ccccagatga gtaatccgaa gttcatccat accacaggtc ctggctattc tgttgtactc
      661 ctgaacaaga gcaaataatt ctgaattagc aaccatgaac tcatcgcaaa ccctctgtat
      721 agcatcacta ttcagaataa taacgtctct tcccgaaaga cgatcaggag tacagaacaa
      781 aactgtcaaa cggctgaagg cctttgctcg tgctgcattg actatatcaa tacgctgcct
      841 aaggatgaaa caccccgacg cctcatcaat attcactcta cccacaccat atgaatgata
      901 aatatttaat gctgaaaaaa ccattagacc gtataacaaa cactcaatca atacttaaca
      961 gaacttttat ttttgacaaa cataaaatat tttcaacaat atcctgagcc aggtatattt
     1021 cagtataagg ctctgccgga aggaatctgg aagaatgaat atggcgcgct gtactggatt
     1081 cgaaccagtg accgattgct tagaaggcaa ttgctctgtc cggctgagct aacaacgctg
     1141 aataccgata atggaccgcc atcggggacc cgcccccgca ccaacaaccc tgttatcgtg
     1201 tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg gtggcccttg ctggatttga
     1261 accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggagc
     1321 agaataataa cggtccgtaa ttaattccgc aataaaaaac ccgctcggcg gcgggttgta
     1381 gaaactcttc taacgtcagg cataaaacgc ccatagttat gacgaattta ccacagattc
     1441 cggaaaaatc aacctagtta ccttttttaa ctgccgctca gcccatgctt cttcaatatc
     1501 aaaccgagtc accagcgcat cata
//
LOCUS       DAJVXP010000292         1516 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVXP010000292 DAJVXP010000000
VERSION     DAJVXP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.298"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>1516)
                     /locus_tag="OQS10_004247"
     CDS             complement(<1..>1516)
                     /locus_tag="OQS10_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2295097.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAJVXP010000293         1501 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVXP010000293 DAJVXP010000000
VERSION     DAJVXP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.299"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(370..714)
                     /locus_tag="OQS10_004248"
     CDS             complement(370..714)
                     /locus_tag="OQS10_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS2295098.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            846..>1501
                     /locus_tag="OQS10_004249"
     CDS             846..>1501
                     /locus_tag="OQS10_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS2295099.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     A"
BASE COUNT          404 a          351 c          375 g          371 t
ORIGIN      
        1 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
       61 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagaaggaga aaattaacgg
      121 tcagtccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
      181 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
      241 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
      301 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
      361 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
      421 acccgcttgt accgtgcagc gcactggcgc atgacccgcg acaacccagg catcccacgc
      481 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
      541 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
      601 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
      661 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
      721 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
      781 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
      841 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
      901 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
      961 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
     1021 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
     1081 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
     1141 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
     1201 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
     1261 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
     1321 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
     1381 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
     1441 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
     1501 c
//
LOCUS       DAJVXP010000294         1495 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVXP010000294 DAJVXP010000000
VERSION     DAJVXP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.300"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(478..1002)
                     /locus_tag="OQS10_004250"
                     /pseudo
     CDS             complement(478..1002)
                     /locus_tag="OQS10_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
     gene            1257..1495
                     /locus_tag="OQS10_004251"
                     /pseudo
     CDS             1257..1495
                     /locus_tag="OQS10_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          429 a          343 c          266 g          457 t
ORIGIN      
        1 aaaataaaca gacattggtg catcattata agctaatgct ttacccattt tacccgcagc
       61 taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac gaccaccaaa
      121 gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg tagagttttg
      181 tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa aataagtctg
      241 cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat cagttgtatc
      301 ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat ttttataatt
      361 gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct tagttaagtc
      421 aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc ctagtgctac
      481 gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa tctcaatggt
      541 ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca gaacttttac
      601 accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt gtgtaatttc
      661 atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct gtactgacaa
      721 ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag tggtctgcgc
      781 aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa tatttgctgt
      841 aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa tacgcaggcc
      901 agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca ggttattctg
      961 ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt ccttatcaat
     1021 tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct tgtatcggca
     1081 cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta acggctgaaa
     1141 tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta ttaaactttg
     1201 cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa ttaggtatgg
     1261 cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg ctaaacaacc
     1321 tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt gacaataccg
     1381 cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca gactgcaaat
     1441 actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag caatg
//
LOCUS       DAJVXP010000295         1431 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVXP010000295 DAJVXP010000000
VERSION     DAJVXP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.302"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            14..439
                     /locus_tag="OQS10_004252"
     CDS             14..439
                     /locus_tag="OQS10_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2295100.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /locus_tag="OQS10_004253"
     CDS             464..>1431
                     /locus_tag="OQS10_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS2295101.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAJVXP010000296         1391 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVXP010000296 DAJVXP010000000
VERSION     DAJVXP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.303"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..233)
                     /locus_tag="OQS10_004254"
     CDS             complement(<1..233)
                     /locus_tag="OQS10_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295102.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDGGLTTAERQRLKELER"
     gene            331..591
                     /locus_tag="OQS10_004255"
     CDS             331..591
                     /locus_tag="OQS10_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295103.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            588..1178
                     /locus_tag="OQS10_004256"
     CDS             588..1178
                     /locus_tag="OQS10_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295104.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(1198..1389)
                     /locus_tag="OQS10_004257"
     CDS             complement(1198..1389)
                     /locus_tag="OQS10_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295105.1"
                     /translation="MTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLM
                     ASFHWWRRKVNRLPPWDDV"
BASE COUNT          314 a          402 c          401 g          274 t
ORIGIN      
        1 cgctccagct ctttcagacg ctgacgttca gcggtggtga gccctccatc accgcccccg
       61 gtatcccgct catgctggcg aacccggaca cgcagagtct ccggcgtaca gccaatcttt
      121 ggagcaatgg aacaaattgt cgcccattgt gagtcatatt cgccctgact ttccagaacc
      181 atacggactg cccgttgacg gacttcgggg gaaaaacgag tatttttagt catcctgttt
      241 acctctttct caggaagttt agtctccagg attcccgggg cggttcaatg atttttacct
      301 gtcagaacat tatcagatgg aacgtgcagg atggatcagg aaatgacgtt ttcactcagc
      361 tatgaacaac tgacccggtt tgcggaaaaa cgcatccggg aatgcaatct ggacagccac
      421 ggcgtaactt atctctgcga gtcagccaaa gccggagccg tgctgatctt ctggcatgaa
      481 ctggccataa atggctatac ctcaatgaat gccataaaga gacaggagat cattgacgcc
      541 gaccatcagc gcctcagaaa gctcatctgg ccggaggatg actggaaatg actcagagcc
      601 gccgaccgtc gccactgcag cggagagtgc tgattgtgct ggctgccctt gatgaaaaac
      661 ggccaggacc cgtgctgacg cgggacatag aacgggtgct ggagcaaagc ggggaagccc
      721 cggtctacgg gccaaacctg cgcgcctcct gccgtcgtct ggaggatgcc ggctggctgc
      781 gtaccctgcg cgctccgaac ctgcagctgg cagtggagct gacggacgcc ggccgggccg
      841 tggcacagcc tctgctgctg gcagaacagg accgcatccg ggcagaacag cgtgcggcag
      901 aagtcgtcgt actgccgctg gtcccggcag caggtctgcc cgcaggcggc acttctgcca
      961 ctgatctggc ggtggagctg aacggcataa cgtatcaggc ctgccgtggg gattttgtgg
     1021 tgcgtctcga cggcagcacc tgtctgcagt tatggaataa agaaggctgg gtggtacgcc
     1081 gggagggcga tccgctggaa gtggcgcagt ggctgccggc ctgtcacgat gcaggaatgg
     1141 aggttcgcgt acagataaat gaaagtgccg ctccgtaaga acacgcctgt gcacggttta
     1201 cacatcatcc catggtggca gtcggttaac tttcctgcgc caccagtgga aagaggccat
     1261 gagcagtccg gacaaaaaac cccccgtgat acttataata atggcctcac ctgccaccat
     1321 tcctgacggc ccccagtaga taaaccacat tgcacatccc caggaaatgc cccataagcc
     1381 ccccgtcagc a
//
LOCUS       DAJVXP010000297         1379 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVXP010000297 DAJVXP010000000
VERSION     DAJVXP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.304"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            3..983
                     /locus_tag="OQS10_004258"
     CDS             3..983
                     /locus_tag="OQS10_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCS2295106.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1379
                     /locus_tag="OQS10_004259"
     CDS             998..>1379
                     /locus_tag="OQS10_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS2295107.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLG"
BASE COUNT          359 a          315 c          365 g          340 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattaggtg
//
LOCUS       DAJVXP010000298         1375 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVXP010000298 DAJVXP010000000
VERSION     DAJVXP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.305"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..369)
                     /locus_tag="OQS10_004260"
     CDS             complement(<1..369)
                     /locus_tag="OQS10_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCS2295108.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQD"
     gene            complement(344..1144)
                     /gene="otsB"
                     /locus_tag="OQS10_004261"
     CDS             complement(344..1144)
                     /gene="otsB"
                     /locus_tag="OQS10_004261"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS2295109.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
BASE COUNT          318 a          369 c          332 g          356 t
ORIGIN      
        1 gtcttgcaac agcggcagta atttatctgc cagcaacgca tttacgcgta gatagccgtc
       61 ccaggcagga cgctgaaatt gcaccagatc gagccgataa tgaaaagcgg gccagagaac
      121 ggcattggag aattggttgt agtattcgtc aaggtcctgt tcgctgaggt taaaagaggc
      181 ccacgtaatg ttaccttttt tcaccttttt tagcggctga tcctcattcc ctgtttcacc
      241 actccagcca aaccacagtc cgcctgcggc tttcagtgcc cccagtatgc caacggcaag
      301 gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc cggttagata ctacgactaa
      361 acgactcata gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg gtggttatca
      421 tttcaagcca gctccagaca tccggcacac ccgccagtcg ccatgatgcc tgagttgcac
      481 ctgtgccaat ttttactgac attccgccca gtcggttaac gactgcgaag ccagattcat
      541 cggttaaatc atcgcccaga aatacgggcg ttcgcccgat aaagggagct tcctgcataa
      601 aagctgcaat tgcctcacct ttactggtac ctctcggttt gatctcgaca acacactttc
      661 cctgctgtaa cgccatttgt ggccagatct gagtaatacg ttgcgctaat gtcattaatg
      721 cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa agccatccct ttcgcctcca
      781 gctccgcgcc gggatactga gcgatgactg tatgcagttg cacgctaata tcacgcgcaa
      841 tcgcatccgg cagatgaacg atatgtgttt taccattgat gtcacggcgc tccgccccat
      901 gcacgcccgc taacgggaag cgataaggtt ttgccagtgc gtcaagctcc accattgagc
      961 gccctgatat caatgccaat gcaccatcac ttgcggttgc cagtagctgt agtccttgca
     1021 gaatattgtc aggcacgacg acctgatcgg gatgcggttt gatttccgcc agcgttccat
     1081 caagatcaaa aaaccaggca tatttcgcgg atagttcagg ggtttcggtt aacggttctg
     1141 tcacccggct ctcctccttc tcttttctta ttgttttgcg cagaacgcgc tttacagaca
     1201 tgtaagtata gacagtgtga cgggggtcgc catttgcaat gaagaacagc caaccgttgt
     1261 ggcggctggc tgtggtggga aaaaacgtta aattgttgtg gaaaaaagca tcagacaatg
     1321 cgtttcgctt tttgcttgta acggtcgaag atcaccgctg ccagcaggat taagc
//
LOCUS       DAJVXP010000299         1374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVXP010000299 DAJVXP010000000
VERSION     DAJVXP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.306"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(50..193)
                     /locus_tag="OQS10_004262"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OQS10_004262"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="OQS10_004263"
     CDS             complement(197..1018)
                     /locus_tag="OQS10_004263"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS2295110.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1374
                     /locus_tag="OQS10_004264"
                     /pseudo
     CDS             1007..1374
                     /locus_tag="OQS10_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    1287..>1374
                     /locus_tag="OQS10_004264"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          357 a          297 c          299 g          421 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggac
//
LOCUS       DAJVXP010000300         1373 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVXP010000300 DAJVXP010000000
VERSION     DAJVXP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.307"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..294)
                     /locus_tag="OQS10_004265"
     CDS             complement(<1..294)
                     /locus_tag="OQS10_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295111.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPP"
     gene            complement(1115..1327)
                     /locus_tag="OQS10_004266"
     CDS             complement(1115..1327)
                     /locus_tag="OQS10_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCS2295112.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          448 a          284 c          258 g          383 t
ORIGIN      
        1 cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc ccagttcaaa
       61 ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata ccgtcaggta
      121 ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc tgaccagaga
      181 gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa ccaccgcaaa
      241 agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg acatagcaac
      301 ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg ggcggatgag
      361 ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat ggttatgtga
      421 tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa caaaataatc
      481 acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat tacagcgagt
      541 aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt attcacaaca
      601 ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga atatacgcta
      661 ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa acactggctt
      721 aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat aataagcaaa
      781 tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca ctgcaccacc
      841 acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt gttcccaaaa
      901 tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc tattgattag
      961 gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc aacatattta
     1021 tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc ctatagataa
     1081 ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg atatttttat
     1141 ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa aactttttga
     1201 gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata gttagtttct
     1261 tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa atcaaaaaac
     1321 ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga ata
//
LOCUS       DAJVXP010000301         1335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVXP010000301 DAJVXP010000000
VERSION     DAJVXP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.308"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..468)
                     /gene="umuC"
                     /locus_tag="OQS10_004267"
     CDS             complement(<1..468)
                     /gene="umuC"
                     /locus_tag="OQS10_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS2295113.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKW"
     gene            complement(468..887)
                     /locus_tag="OQS10_004268"
     CDS             complement(468..887)
                     /locus_tag="OQS10_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS2295114.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1260..>1335
                     /locus_tag="OQS10_004269"
     CDS             1260..>1335
                     /locus_tag="OQS10_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCS2295115.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALD"
BASE COUNT          405 a          317 c          274 g          339 t
ORIGIN      
        1 ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg cgatccccat
       61 accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc caaaatcagt
      121 cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa tactgtaaat
      181 ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca tgtctgcgta
      241 aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat ctttttgttt
      301 gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc gggcgataac
      361 gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag ggcgaaacac
      421 cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca gcgcatcgcc
      481 ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact gatggtaatg
      541 ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg caattttttc
      601 accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc ggtaatagcg
      661 ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga atcaccactt
      721 gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag atcgatgcgc
      781 tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc gctaaatagc
      841 ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat aatctgcctg
      901 aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat actgattcag
      961 gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc ctgtgctcac
     1021 gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc ggcaaaaaaa
     1081 tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat catattaata
     1141 gaaataaaga cattgacgca tcccgcccgg ctaactatga attagatgaa gtaaaattta
     1201 ttaatagttg taaaacagga gtttcattac aatttatata tttaaagagg cgaatgatta
     1261 tgactgaaat cgttgcagat aaaacggtag aagtagttaa aaacgcaatc gaaaccgcag
     1321 atggagcatt agatc
//
LOCUS       DAJVXP010000302         1290 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVXP010000302 DAJVXP010000000
VERSION     DAJVXP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.309"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..1112
                     /locus_tag="OQS10_004270"
                     /pseudo
     CDS             1..1112
                     /locus_tag="OQS10_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          352 a          262 c          398 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata
//
LOCUS       DAJVXP010000303         1282 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVXP010000303 DAJVXP010000000
VERSION     DAJVXP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.310"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            132..1151
                     /locus_tag="OQS10_004271"
     CDS             132..1151
                     /locus_tag="OQS10_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS2295116.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAJVXP010000304         1266 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVXP010000304 DAJVXP010000000
VERSION     DAJVXP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.311"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            76..1266
                     /gene="caiB"
                     /locus_tag="OQS10_004272"
                     /pseudo
     CDS             76..1266
                     /gene="caiB"
                     /locus_tag="OQS10_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT          328 a          344 c          320 g          274 t
ORIGIN      
        1 acgaatgtag gccggataaa gcgtttacgc cgcatccggc agtcatgcac tacataaatt
       61 tttcaggagc taataatgga tcatctaccc atgccgaaat tcgggccgtt ggccggattg
      121 cgcgttgtct tctccggtat cgaaatcgcc gggccgttcg ccgggcaaat gttcgcagaa
      181 tggggtgcgg aagttatctg gatcgagaac gtcgcctagg ccgacaccat tcgcgttcaa
      241 ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa
      301 gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc
      361 agcaaaggtc cggcctttgc ccgtcgtggc attaccgatg aagtactgtg gcagcacaac
      421 ccgaaactgg ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc
      481 aatcttccgg cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt
      541 gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc
      601 gccaccacgg cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt
      661 atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac
      721 ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt
      781 tgcggtctgt ataaatgcgc cgacggctac atcgtgatgg aactggtggg cattacccaa
      841 attgaagagt gctttaaaga tattgacctc gcacatctac tcggtacgcc ggaaatcccg
      901 gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa
      961 ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg
     1021 aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt
     1081 gcccgtgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac
     1141 atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc
     1201 atggacacgg ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg
     1261 gtcagc
//
LOCUS       DAJVXP010000305         1261 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVXP010000305 DAJVXP010000000
VERSION     DAJVXP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.312"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            234..905
                     /gene="queE"
                     /locus_tag="OQS10_004273"
     CDS             234..905
                     /gene="queE"
                     /locus_tag="OQS10_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS2295117.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          353 a          246 c          280 g          382 t
ORIGIN      
        1 tcgttatcat taaaaactaa tacagaaaaa ataaatataa ttttggtgaa tgttttgaca
       61 tgattaaatc cattttcttt attttcttct gacataatac gttactccct ttaattatta
      121 acgatatgtt tcgcattttt ctttaaatct gcctgtaaca gaatgttaat aagacatgat
      181 tgttgtggca ggatctgcaa taattaacaa ttatccactt cacagagaat gctatgcagt
      241 acccgattaa cgagatgttc cagaccctgc aaggtgaggg ttactttacc ggcgtccccg
      301 ccatttttat tcgtttacag ggatgcccgg ttggctgtgc ctggtgcgac accaaacaca
      361 cctgggaaaa gcttgaggat cgggaagtct cccttttcag cattctggcg aagaccaaag
      421 agagtgataa gtggggggcg gcgagcagtg aagacttgtt ggctgtcatt agtcgccagg
      481 gatacaccgc gcggcatgtg gtgattacgg gtggtgagcc ttgcattcat gatttgctac
      541 cactgactga tctgctcgaa aagaacggtt ttagctgcca gatcgaaacc agtggtactc
      601 atgaggtacg ctgcacaccg aatacctggg ttaccgtatc gccaaagctg aacatgcgcg
      661 gcggctatga cgtgttgtca caggcactgg agcgagccaa cgaaatcaag catccagtgg
      721 ggcgcgtacg cgatattgaa gcactggatg aactattggc aacgctgacc gatgataaac
      781 cgcgagtcat tgcactgcag ccgattagcc aaaaggatga tgccacacgt ttgtgcattg
      841 aaacctgcat tgcgcgtaat tggcgtttgt cgatgcaaac acataaatat ctaaatattg
      901 cctgattaaa catttataag cgttataaat gggtggaacc tgtaaagact tctactcatt
      961 tatattgttt gtcgcctctg aaaactcctc cattttaccc atccagggct aatcattagc
     1021 attctctaca aattctgtgg cattaatttt tcgctggagt gaaaattatt gcggtaaagt
     1081 ttggtagatt ttaatttgta tagagttatt ttaaatattt acctttttaa tcaatgaatt
     1141 aagtactctt taacataatg gatgtgttgt ttgtgtgata ctgtaaagtt ggtagattgt
     1201 gactggctta aaaaatcatt aattaataat aggttatgtt tagagtgtcc cccgcgccag
     1261 c
//
LOCUS       DAJVXP010000306         1238 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVXP010000306 DAJVXP010000000
VERSION     DAJVXP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.313"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..1235
                     /locus_tag="OQS10_004274"
     CDS             <1..1235
                     /locus_tag="OQS10_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2295118.1"
                     /translation="IIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAPGWL
                     ELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRISKTK
                     KPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFYAVQ
                     QLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNKESR
                     CSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTETLI
                     QLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEAQII
                     IKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDMLIF
                     GEPVTRIYPAGDMEEVDA"
BASE COUNT          400 a          225 c          292 g          321 t
ORIGIN      
        1 acatcatcat agctgagaga gcacaccaag aatctcttgt taagcaaaag cgtatagctc
       61 aggtctggaa ccataaaaca cagcttgcaa gagaacttaa gaagcctatg ggtaagcaag
      121 cccccggatg gttagagcta tcagaagatg gttcatacta catcgttgat gaagataaag
      181 cgtcactggt gaatatcatc tatgacaagc gtctttctgg tatgtcgatg tttgctatct
      241 gtaaatggct aaacgaacaa ggttacccga ccattaatca gcgtaaggtc agaatatcca
      301 agactaagaa accggatggc aactggtcag cattaagcgt taaacacatc ctgacttcac
      361 gaagtgtatt aggttacctt cccgctaaga tttctacaga agaccgtaaa acagtgttac
      421 gtgaagaaat tgaagggttc tatcctcaga ttgtcacaga ctctaagttt tacgctgtac
      481 agcaactgtt agaggaaaca gggaagggga aaacatccag cggtgaacac tggctatatg
      541 tgaacatact gaaggggctt atccgttgta agtgtgggct tgtaatgact cctacaggga
      601 tacgtaaacc cgtttatcaa ggtacttacc gttgcaatgg taataaggag tcccgttgta
      661 gctacggtac agtgtccaga aagttacttg atacacagct ttgtagtcgt ctgtttagca
      721 aactgtcaca gctacacgat gaagctaccg atacagctaa attagatgaa ctacaaagaa
      781 gattaaacac cgttgatggt gaactggaaa aactcactga gacgctgata cagcttccta
      841 acatcactca gattcaagaa gcactacggg ttaaacagga agaaaaagat gaactgatag
      901 ttcagttatc acgagaaaag gcgagggtaa aatctgtatc aagcttggat ttaagcggtc
      961 ttgatatgga gtctgtagag gggagaactg aagcccagat tattattaag cgtctggtga
     1021 aagagattgt tgtttctggt aatgaaaagc tggtggatat atatcttcac aatggaaata
     1081 tgattcgtgg ttttcctctt gatggtaaag atgaccacac cttaacgctt gaagaagcaa
     1141 ccgatgagat gcagtcatta gatgatatgc ttatttttgg tgaaccagta acaaggattt
     1201 atcctgctgg tgatatggaa gaggtggacg cttagtat
//
LOCUS       DAJVXP010000307         1222 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVXP010000307 DAJVXP010000000
VERSION     DAJVXP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.314"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..695)
                     /locus_tag="OQS10_004275"
     CDS             complement(<1..695)
                     /locus_tag="OQS10_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2295119.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1222)
                     /locus_tag="OQS10_004276"
     CDS             complement(1011..>1222)
                     /locus_tag="OQS10_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCS2295120.1"
                     /translation="TLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHI
                     LSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          354 a          210 c          259 g          399 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatattctg
      721 ataaaatgtg atactgggaa ataataagct gaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaaga gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcgg aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgtggctc cgtatagagt tc
//
LOCUS       DAJVXP010000308         1216 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVXP010000308 DAJVXP010000000
VERSION     DAJVXP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.315"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..399)
                     /locus_tag="OQS10_004277"
     CDS             complement(<1..399)
                     /locus_tag="OQS10_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS2295121.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIR"
     gene            complement(768..>1216)
                     /locus_tag="OQS10_004278"
     CDS             complement(768..>1216)
                     /locus_tag="OQS10_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS2295122.1"
                     /translation="DEAVSGSVKASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDV
                     LSTVKALQAKGVTVISLREGFDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAK
                     AEGVHMGRPVKASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          354 a          274 c          213 g          375 t
ORIGIN      
        1 cctaatagta agcctgttat gccctgctgg tggaaggtat ttaactgcac gatgaagaat
       61 acggatttca tgacagtggt tattgctgta aatgtgccta agccagtagc tggtgttggc
      121 tctatgcggt atcaggaaag ctactaaatc cgcttcagac gcttttaaga ggaacgacgg
      181 aatatcgctg tacggcgggt tacagaagac cttttcacta acccatgatt gacggcttat
      241 atcatcggtg aatcgtggta acagcgtgtc atgctcagtt gcagcaccat ctagactgaa
      301 aataaattca cggtgtaagt tcttaaataa caaaaatgga gttctccagt aatcagaacc
      361 cccatgatgc ttcccgatta aaatatgctc aattgacata agaaataaaa atttttattg
      421 caatcactct tgacaaggtt tactctattt taagagatgc ttaacaggtt ttctaaacgg
      481 atatcctttc tgggatatct atcttttatc ttaaaagttt ttacagatac tttaaagata
      541 tctgtatata ttctgtacag taacttttca gagtctgaaa atttttataa aaaatgacta
      601 gccctctttg aatctcatct tccccgaatc ttcgaaaata tctgtctagt tgattcggta
      661 gaatctacct catgatgtat acgtgtttag tacccgtcac accagctatg tgaccccctt
      721 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      781 ctgtaataag ttgctctaga gattcctagc tcttcctgaa tctgcaacct cgttttacct
      841 tgtgaaataa gcatctgtac agcctctgaa gaagctttta caggtcttcc catatgtacc
      901 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      961 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
     1021 aatccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
     1081 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
     1141 tctcgtgcat aagctaacaa actgctgaaa ccgtttctta aagaagcttt gacagaacca
     1201 ctgacagctt catcag
//
LOCUS       DAJVXP010000309         1212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVXP010000309 DAJVXP010000000
VERSION     DAJVXP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.316"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            229..>1212
                     /gene="gspA"
                     /locus_tag="OQS10_004279"
     CDS             229..>1212
                     /gene="gspA"
                     /locus_tag="OQS10_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCS2295123.1"
                     /translation="MSTRREVILSWLREKRQTLRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGN"
BASE COUNT          329 a          249 c          308 g          326 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgatagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga ccttgcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg acattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc agcttgccgt ggtaatgagt
      961 ggaaccatta tcgctattac ctgtggttgg ctactgctgt cctctttcac agctccgttc
     1021 cccgttcctg cgtggctggt acccgtaacg ccagtagtta aacaagatat gactaaggat
     1081 attgctcatg tggtgatgcg cgatagtgag gcgctaagcg tgttatacgg cgtatggggg
     1141 tacgaggtcc cagctgattc ggcctggtgc gatcaggcag taagagcggg gctggcatgt
     1201 aaaagtggga at
//
LOCUS       DAJVXP010000310         1207 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVXP010000310 DAJVXP010000000
VERSION     DAJVXP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.317"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            52..>1207
                     /locus_tag="OQS10_004280"
     CDS             join(52..301,301..>1207)
                     /locus_tag="OQS10_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS2295124.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          343 a          294 c          332 g          238 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gtgccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg
     1141 aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact
     1201 cggtggc
//
LOCUS       DAJVXP010000311         1188 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVXP010000311 DAJVXP010000000
VERSION     DAJVXP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.318"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..241)
                     /locus_tag="OQS10_004281"
     CDS             complement(<1..241)
                     /locus_tag="OQS10_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS2295125.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLA"
     gene            complement(367..945)
                     /gene="flhC"
                     /locus_tag="OQS10_004282"
     CDS             complement(367..945)
                     /gene="flhC"
                     /locus_tag="OQS10_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS2295126.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(948..>1188)
                     /locus_tag="OQS10_004283"
     CDS             complement(948..>1188)
                     /locus_tag="OQS10_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS2295127.1"
                     /translation="INEEMATTLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQD
                     SRVDDLQQIHTGIMLSTRLLNDVNQPEEALRKKRA"
BASE COUNT          297 a          298 c          288 g          305 t
ORIGIN      
        1 gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca
       61 gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc
      121 ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg
      181 tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca
      241 cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc
      301 gattggggca aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgcctcaccg
      361 tatcagttaa acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg
      421 attctgggaa agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc
      481 aaagctgcca acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct
      541 ggaaagttgc agtaatccac tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc
      601 cagcagtggt ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat
      661 caccgcatcg acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa
      721 catcgaagca tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat
      781 gcctttcggc ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact
      841 taactgtgtt tcgctttcca gcatctgcaa acgagcgccc agggtaatca attccattgc
      901 cagctgaata tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc
      961 ttgcgcagcg cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg
     1021 ccggtatgaa tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc
     1081 tggtggctgt caaaacggaa gtgacaaacc agttgattgg tttctgccag cttaaccatt
     1141 tgcggaagag tcagtgccgc taacgttgtc gccatttctt catttatg
//
LOCUS       DAJVXP010000312         1169 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVXP010000312 DAJVXP010000000
VERSION     DAJVXP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.319"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..172
                     /locus_tag="OQS10_004284"
     CDS             <1..172
                     /locus_tag="OQS10_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295128.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="OQS10_004285"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="OQS10_004285"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS2295129.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..1169
                     /locus_tag="OQS10_004286"
                     /pseudo
     CDS             955..1169
                     /locus_tag="OQS10_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Plays a crucial role on both purine and pyrimidine
                     metabolism; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
BASE COUNT          314 a          246 c          307 g          302 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccg
//
LOCUS       DAJVXP010000313         1157 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVXP010000313 DAJVXP010000000
VERSION     DAJVXP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.320"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..262)
                     /locus_tag="OQS10_004287"
     CDS             complement(<1..262)
                     /locus_tag="OQS10_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS2295130.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPF"
     gene            complement(310..882)
                     /locus_tag="OQS10_004288"
     CDS             complement(310..882)
                     /locus_tag="OQS10_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCS2295131.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          370 a          241 c          210 g          336 t
ORIGIN      
        1 taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc tggatgagat
       61 aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta ttacttaagt
      121 aaaccacccg cgtaccacca atgacaacgc ctgcataagt taatgctggt tggcctaaaa
      181 aaacgctgag tataatcgcg gtaaataact tacatgaaac tgttgtttta aagagcctga
      241 ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa taaaaaggcc
      301 cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg ccgtaccagc
      361 cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag ccttgccttc
      421 agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat tatcggactg
      481 gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac cggtaaaatc
      541 accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc cgccaggcgc
      601 ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat tttcaagttt
      661 aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat ttttactgac
      721 tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag tagtgtcgac
      781 aatttcaccg acgaagtgaa caacaccatc ttctgccagt gcatgcccgg ccagcattag
      841 agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc cattgatata
      901 atatatcctt taccatgaat catcacttat attaataagt gtatttttga ttcaaaggaa
      961 tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa aataaaatat
     1021 aaattcattg tttaaatatt atttaaacag taaaaaatca agtttgttat cagacattaa
     1081 tgataattca gaatatattt atatagaaag atagataaat ttattaatgt cagttataaa
     1141 aaagggccac cggtcgg
//
LOCUS       DAJVXP010000314         1148 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVXP010000314 DAJVXP010000000
VERSION     DAJVXP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.321"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            272..>1148
                     /gene="nadA"
                     /locus_tag="OQS10_004289"
     CDS             272..>1148
                     /gene="nadA"
                     /locus_tag="OQS10_004289"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS2295132.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          311 a          276 c          276 g          285 t
ORIGIN      
        1 gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatt
       61 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
      121 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      181 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      241 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      301 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      361 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      421 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      481 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      541 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      601 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      661 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      721 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      781 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      841 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      901 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      961 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
     1021 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1081 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1141 ctgccgca
//
LOCUS       DAJVXP010000315         1127 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVXP010000315 DAJVXP010000000
VERSION     DAJVXP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.322"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..500)
                     /locus_tag="OQS10_004290"
     CDS             complement(<1..500)
                     /locus_tag="OQS10_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HCS2295133.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYF"
     gene            complement(557..>1127)
                     /locus_tag="OQS10_004291"
     CDS             complement(557..>1127)
                     /locus_tag="OQS10_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCS2295134.1"
                     /translation="ESCPHYFVLDTDQFEEIGTLAKCSPPIRDLENQKGMWEKLFNGE
                     IDCLVSDHSPCPPEMKAGNIMEAWGGIAGLQNCMDVMFDEAVQKRGMSLPMFGKLMAT
                     NAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHKVSPYVGRTIGARITK
                     TILRGDVIYDIEQGFPVAPKGQFILKHQQ"
BASE COUNT          306 a          291 c          283 g          247 t
ORIGIN      
        1 ttaaaatatg ggtcggctat gccaaacggc agcccgagca tacctttgaa aaagatagtg
       61 gtcagctgtg cgcccagcat caacataaac aagaccatca ccgacggcgt aaataaccgt
      121 gccaggcgat ggcctaatcc gctaaaacca atcaacatcg tcagcacgcc ggagagtgca
      181 atccccaccg ccagactggt agcgatatcg ttgatcggtg tcccgcgcga tgcttctcca
      241 agggtgatag taaggatggt tccccaccac aggccacccg gcccttccat aatagcgcga
      301 cgatgaccgc agaacgcctg agcgaagcag gccagtgcgg tagcaagaaa agcatattgc
      361 gtgagcgtaa gcaggctact ttgcggcaac tgaaaagcag aaagtagcgt aggaggaacc
      421 acaaccgtgt tgcaaaaaat aaagaaaaac cactgaaatc ctgataacag gctttcgcgg
      481 ctgactgcaa aattgaacat aaacacacct taaccggaga atgcccgccg caaggacggg
      541 cattgcaggg gccagattac tgctgatgtt taaggataaa ttgacctttc ggcgcaacag
      601 ggaagccctg ttcaatgtcg taaatcacat caccacgtaa gatggttttc gtgatacgcg
      661 cgccaatggt acggccaaca tacgggctga ctttgtggcg atattccaga tcgtcattgg
      721 taagaacata gctgctattc ggctgaatga agacgaagtc ggcatctttt cctggggcga
      781 tacggccttt ttgctgcaga ccgaaaatat ctgctgcgtt agtcgccatt aatttgccga
      841 acattggcag agacattccg cgtttctgta ccgcttcatc gaacatcacg tccatacagt
      901 tttgcagacc ggcaataccg ccccatgctt ccatgatgtt gccggctttc atttccggag
      961 ggcatggtga gtggtcggaa accaggcagt cgatttcacc gttaaacagt ttttcccaca
     1021 tgcctttctg gttttccaga tcgcggatcg gcggtgaaca cttcgccaga gtaccgattt
     1081 cttcgaactg atcggtatcc agtacaaagt aatgcgggca ggattca
//
LOCUS       DAJVXP010000316         1116 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVXP010000316 DAJVXP010000000
VERSION     DAJVXP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.323"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            84..1115
                     /locus_tag="OQS10_004292"
     CDS             84..1115
                     /locus_tag="OQS10_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS2295135.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          300 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagc
//
LOCUS       DAJVXP010000317         1109 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVXP010000317 DAJVXP010000000
VERSION     DAJVXP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.324"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            759..1055
                     /locus_tag="OQS10_004293"
     CDS             759..1055
                     /locus_tag="OQS10_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS2295136.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          330 a          213 c          236 g          330 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggaagtcct tttaagactg gttatgaagg agctatgagg
      121 ttgattaggg gttggtacaa gcccaactaa ccgaatcaac tatgaagatt ttaggagggt
      181 aactgtaacc atcactttct aactttcacg taggaaattg ttaagtggtt tgatagttac
      241 accatgtgac caagcatcta tatatcgtta tatagccctg tatatagcac tatatgtagt
      301 gtcgagagag gaacgactaa cacgatacag tttaccctga aaagagactt cacagattct
      361 ttatatattt caataagtta gggtaaaaag ggactatacc gagactgtat agaatctgtg
      421 tagaattgca tagatacttg gaaatctact attaaattgc atagggactc acctgatatt
      481 gtcctgtttt taacaggtct cctagaaaac cgaatagact tcattcaagg gtcattgaag
      541 tcataatcat ttcagtagaa aaaccaagtc tcgattttaa atgcaaatag caatcatatt
      601 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
      661 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
      721 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
      781 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
      841 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
      901 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
      961 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
     1021 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     1081 ggtttttgca accgtgttct tgtttggcc
//
LOCUS       DAJVXP010000318         1066 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVXP010000318 DAJVXP010000000
VERSION     DAJVXP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.325"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..369)
                     /locus_tag="OQS10_004294"
                     /pseudo
     CDS             complement(1..369)
                     /locus_tag="OQS10_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(509..>1066)
                     /locus_tag="OQS10_004295"
     CDS             complement(509..>1066)
                     /locus_tag="OQS10_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2295137.1"
                     /translation="AVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRL
                     HIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKF
                     ATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVFKAG
                     LRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          300 a          241 c          184 g          341 t
ORIGIN      
        1 ccctgcggca ctcttgtcat aagagtgccg gagcgttttt ccatcaattg caatgacgtc
       61 tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt gaaattttgc
      121 aggactgata caggatacaa ctctggcgat ggtatcgtga acaggaatac cattttcaaa
      181 atcaccatat tgttttaaaa aatcgagatg tgtttcccca aaatcctcta tatcttccca
      241 gccttctgca ccagaaataa cggcacaaat agtcaacagt agaatatccg ataatttatg
      301 ttccactttc caggcttgtt tttaatcggg gataatagaa atatgttcca tcaatttttt
      361 aagttccatt ttgttctcct taattatgta aggagtattt gatcatgtat aagcaataaa
      421 aaacagcttc aggtaataag gaatatctca atttttaaac ataaaatgcg aattatttag
      481 tacaaaaagt gaactcctca gggcaagatt acgaaagtcc gctccccgca aggactgacg
      541 cgagatagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata
      601 ccttatcatt cgtcaaaaca ttaatagcaa tgtgccgtat ccctgaaaat aattctgctg
      661 catttcctct tcttattttg cagtcgtctt cattcattac cacgtccaga cgccagtgca
      721 gattattctc cacgtgccag tgatttcgaa tcgctgtcgc aaacttctca gcggttaaat
      781 cagcagaact gatataatat ctgaccatca tttcgggctc tttcttttgc tctgctatta
      841 ttgagcgaaa ggagactgcc acgcataatt ttttcagccc tttccattca aacgtgaaat
      901 caataagttc atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
      961 tcttttcact cattgcggaa ctgtcatgcg ctggattatt taattctgtc agcggaaatt
     1021 ttttctcaaa ggctttattt agccgcccct ggtttccttt taccgc
//
LOCUS       DAJVXP010000319         1024 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVXP010000319 DAJVXP010000000
VERSION     DAJVXP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.326"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            54..1024
                     /locus_tag="OQS10_004296"
                     /pseudo
     CDS             54..1024
                     /locus_tag="OQS10_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          262 a          262 c          231 g          269 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 atta
//
LOCUS       DAJVXP010000320         1023 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVXP010000320 DAJVXP010000000
VERSION     DAJVXP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.327"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(77..865)
                     /locus_tag="OQS10_004297"
     CDS             complement(77..865)
                     /locus_tag="OQS10_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2295138.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="OQS10_004298"
     CDS             complement(905..>1023)
                     /locus_tag="OQS10_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS2295139.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAJVXP010000321         1001 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVXP010000321 DAJVXP010000000
VERSION     DAJVXP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.328"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..82
                     /locus_tag="OQS10_004299"
     CDS             <1..82
                     /locus_tag="OQS10_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS2295140.1"
                     /translation="WNWILFRKFSSPEQVENVAAQLLEFA"
     gene            82..372
                     /locus_tag="OQS10_004300"
     CDS             82..372
                     /locus_tag="OQS10_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS2295141.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            369..923
                     /locus_tag="OQS10_004301"
     CDS             369..923
                     /locus_tag="OQS10_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS2295142.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          289 a          192 c          287 g          233 t
ORIGIN      
        1 gtggaactgg attctgttcc gtaaattttc ctctccggaa caagtcgaaa atgtggccgc
       61 gcagctgctg gagtttgcgt aatggtggat ttacgtaaag cggcgcgggg gcagatgtgc
      121 accgtcagaa ttcctggcta ctgcaatcac gatccggaaa cgtctgtgct ggcgcattac
      181 cgactggcgg gaacgtgcgg aacagcgata aagccacacg atatgcaggc agcgattgcc
      241 tgtagctcgt gccacgattt aatcgacggg cgggtaaaaa ccagcgatta caccaaagaa
      301 gaattacgcc tgatgcatgc agaaggtgtt tttcgcacac aagaaatctg gagaaaggag
      361 ggatatttat gatttaccca acgaatacag gaaaaagcgg agaacacctt cgtctcacca
      421 cgctggaaag tgtctggatt cagggaaaac tacgtatgtg ggggcgctgg tcgtatattg
      481 gtggcggtaa gacggggaat atgtttaacc tgatgttgac ctctaaaaag ctgacaaaaa
      541 cggcaattaa cgaggcgctc cggaggatga aaaaagcagg tctgaacaag tctgaacttg
      601 aggctttttt gcgggatatg attaacggta agcaaaagag ctggctggcg cattgtactg
      661 atgcagaggc gttatgtatt gatcgggtca taagtgaggt gctggcagag catccaggat
      721 tgattagcgt ccttcggcaa cggtatgagg ggcgggggat gaccaaacgc aaaatggctg
      781 aactgctgaa tgatgcacac ccgaaatgga gtttaagaac ctgtgaaaga cgcattgagc
      841 attggctaaa ggtggcagaa tttattttgt acaaaccaat ggttatggct tttggtatag
      901 agaaaaaagt tattgctttt tgacgtaaaa actgcttcaa ttcttgtacg cttcgcaaag
      961 ctgtaccgcg aggcgaatag cagacatgga catttgaaag a
//
LOCUS       DAJVXP010000322          999 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVXP010000322 DAJVXP010000000
VERSION     DAJVXP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.329"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>999
                     /locus_tag="OQS10_004302"
     CDS             <1..>999
                     /locus_tag="OQS10_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCS2295143.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          233 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattgg
//
LOCUS       DAJVXP010000323          978 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVXP010000323 DAJVXP010000000
VERSION     DAJVXP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.330"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..495)
                     /gene="fliA"
                     /locus_tag="OQS10_004303"
     CDS             complement(<1..495)
                     /gene="fliA"
                     /locus_tag="OQS10_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS2295144.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQ"
     gene            complement(660..>978)
                     /locus_tag="OQS10_004304"
     CDS             complement(660..>978)
                     /locus_tag="OQS10_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS2295145.1"
                     /translation="KGAELSASDLKALATTNPLSKLDEALAKVDKLRSSLGAVQNRFD
                     SAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLS
                     LLR"
BASE COUNT          220 a          243 c          264 g          251 t
ORIGIN      
        1 ctgatgatca tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta
       61 ggagaagagc tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat
      121 ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg
      181 ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg
      241 gctgcgaagt tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc
      301 cgttccttgt agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg
      361 tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg
      421 acgcaccagc gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt
      481 atagagtgaa ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt
      541 ttctgcaggt tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac
      601 cggtaaattg cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat
      661 taacgcagca gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta
      721 cccgcttgtt gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca
      781 tcttctatac ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg
      841 gcagagtcga aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc
      901 aaagcttcgt ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca
      961 ctcagttcag cacctttg
//
LOCUS       DAJVXP010000324          976 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVXP010000324 DAJVXP010000000
VERSION     DAJVXP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.331"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..613
                     /locus_tag="OQS10_004305"
                     /pseudo
     CDS             1..613
                     /locus_tag="OQS10_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          366 a          178 c          161 g          271 t
ORIGIN      
        1 atgttccagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
       61 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
      121 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
      181 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
      241 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
      301 ttaccagaga tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat
      361 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac
      421 tatagattta aacatagaag actctagctc agaggaattc ctttttgata atagcctcga
      481 gaaagacgaa aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc
      541 atataactca gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt
      601 aaatgcaata tagacataat aacttcttac aaaatctata attaataccg tagatattca
      661 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      721 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt
      781 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag
      841 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      901 cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc
      961 caaaagcaaa aacccg
//
LOCUS       DAJVXP010000325          974 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVXP010000325 DAJVXP010000000
VERSION     DAJVXP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.332"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            16..>974
                     /locus_tag="OQS10_004306"
     CDS             16..>974
                     /locus_tag="OQS10_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS2295146.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPT"
BASE COUNT          209 a          232 c          307 g          226 t
ORIGIN      
        1 taaggctaaa aaatcatgac gataagtttt aatactgttc cgtcgaatac gctggtgccg
       61 ttgttctacg ccgagatgaa caattctgcg gcaaatacag cggtgaccag cgcgcctgca
      121 ttactgatcg ggcatgccag caacgatgca gccattgagg ttaacagcct ggtgctgatg
      181 ccatcggcag attatgcccg ccagatttgt ggggcgggga gccagctggc gcgtatggtg
      241 gaagtctacc gtcagacaga tcctttcggt gaactgtatg ttattgcagt accggaagcc
      301 agaggggcgg cggcgacggt cagggtgacg gttgccggag aagcagagga aagcggcacc
      361 ctgagtctgt atgtcgggcg ctcccgtgta caggtgcctg tggtgaatgg cgatgatgcc
      421 actgcggttg ccaccgcgat taaggaagcg gtaaacgggg ttatcaccct gccgtttacg
      481 gcgtcatctg atgcaggtgt ggtgacgctg actgcccgtc ataaggggct gtatggtaat
      541 gagttaccgg tctgtctgaa ttattatggt tctggtggtg gtgagattct gccagcaggg
      601 cttcgggtgg tgacggaagt cggtgctgcc ggtagcggag cgcctgatct taccgccgct
      661 gttgctgcta tgggcgatga ggcattcgac tttatcggtc tgccgttcaa cgatgccgcc
      721 tccatcaata tgatgatgac cgaaatgaat gacagcagcg gtcgctggag ttatgcgcgc
      781 cagttatacg ggcatggcta taccgcaaaa ctgggaacgc tgtcagagct ggttggtgcc
      841 ggagatatgc ataatcagca acatatcacg cttgccggtt acgaaaaaga aacccagtcg
      901 cctgtcgatg aactggttgc cagtcgcctt gcccgtgaag cggtatttat ccggaatgat
      961 cctgcccgtc cgac
//
LOCUS       DAJVXP010000326          967 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVXP010000326 DAJVXP010000000
VERSION     DAJVXP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.333"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..120)
                     /locus_tag="OQS10_004307"
     CDS             complement(<1..120)
                     /locus_tag="OQS10_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS2295147.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHN"
     gene            complement(132..824)
                     /gene="rutB"
                     /locus_tag="OQS10_004308"
     CDS             complement(132..824)
                     /gene="rutB"
                     /locus_tag="OQS10_004308"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS2295148.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(824..>967)
                     /locus_tag="OQS10_004309"
     CDS             complement(824..>967)
                     /locus_tag="OQS10_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS2295149.1"
                     /translation="EVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQ
                     EVA"
BASE COUNT          226 a          269 c          271 g          201 t
ORIGIN      
        1 attatgttga tcaaaagcca gcgtaccgga gacatacacc acgccatcag ccagcgtgcc
       61 gggaacgaag ggggccagcg gtgcgctgct gccagcggga ataattacgg attttggcat
      121 cgttaaactc cttaagcgat acgagcaaag gacgtgggag aaagcgcgtc gcagaacgtt
      181 tcgacgtcgc tgacccagcc aaaaaaggtt tcgatattga acaacgcggc tttctgtgca
      241 aattcaggcc ccgcctggtg tgttgcgtct tccagcacca cgccgaaata ctccagaaaa
      301 aagccatcgc gtagcgtcga ttcgacgcag acgttggtag cgataccggt gaaaaccaga
      361 tggcgtattc cacggctgcg caaaatgctg tccagcggcg tattgaagaa accgctgtag
      421 cgcggcttcg gcagcacaat atcgccaggc tgcggcacca gttcatccac cagttgataa
      481 tcccaggagc ctttcgccag caatttcccc tgcagctgcg gctgcttacg catggttttc
      541 agggcgttcg atttatgaaa attcggtgag ccgggtccgc cagcttcgac atactgttca
      601 tcccagccat tttgaaacca gatgatcagc atccctgccg ctcgcgctgc ggtcacggcg
      661 gtttgaatgt tggcaatgac cgggcgagtg gttgagacat caaacccggc gagatctaag
      721 tagccgcctg gcgtggcata agcgttttgc atatccacca cgatcagcgc actttgctgc
      781 ggatcgaagg taatggcttc cggtcgagcg gttaaggtcg tcatcatgcc acctcctgag
      841 tcagcgcagg gagatgggcg cggcactgca tcagtggttg aatgcgctcg ccgaaggttt
      901 cgattcccga cagaaaatca tcgaaggtca acagcacgcc ttcggcacca ggcacgcttg
      961 cgacttc
//
LOCUS       DAJVXP010000327          960 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVXP010000327 DAJVXP010000000
VERSION     DAJVXP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.334"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>960
                     /locus_tag="OQS10_004310"
     CDS             <1..>960
                     /locus_tag="OQS10_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HCS2295150.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          273 a          173 c          266 g          248 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggtaa
//
LOCUS       DAJVXP010000328          956 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVXP010000328 DAJVXP010000000
VERSION     DAJVXP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.335"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>956)
                     /gene="cadA"
                     /locus_tag="OQS10_004311"
     CDS             complement(<1..>956)
                     /gene="cadA"
                     /locus_tag="OQS10_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCS2295151.1"
                     /translation="IRELHRALERLNFQIVYPNDRDDLLKLIENNARLCGVIFDWDKY
                     NLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISFFEYALGAAEDIANKIKQT
                     TDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQKSPVGSLFYDFFGPNTMKS
                     DISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTNGTSTANKIVGMYSAPAGS
                     TILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIPQSEFQHAAIAKRVKETPN
                     ATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWVPYT"
BASE COUNT          255 a          204 c          239 g          258 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggat
//
LOCUS       DAJVXP010000329          951 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVXP010000329 DAJVXP010000000
VERSION     DAJVXP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.336"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..951
                     /locus_tag="OQS10_004312"
                     /pseudo
     CDS             1..951
                     /locus_tag="OQS10_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          233 c          289 g          195 t
ORIGIN      
        1 ttcctgcctc tgaacttgct gccgccatga agcagattaa agaactccag cgcctgctcg
       61 gcaagaaaac gatggaaaat gaactcctca aagaagccgt tgaatatgga cgggcaaaaa
      121 agtggatagc gcacgcgccc ttattgcccg gggatgggga gtaagcttag tcagccgttg
      181 tctccgggtg tcgcgtgcgc agttgcacgt cattctcaga cgaaccgatg actggatgga
      241 tggccgccgc agtcgtcaca ctgatgatac ggatgtgctt ctccgtatac accatgttat
      301 cggagagctg cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga
      361 acttgatggt atgcctgcga tcaatgccaa acgtgtttac cggatcatgc gccagaatgc
      421 gctgttgctt gagcgaaaac ctgctgtacc gccatcgaaa cgggcacata caggcagagt
      481 ggccgtgaaa gaaagcaatc agcgatggtg ctctgacggg ttcgagttct gctgtgataa
      541 cggagagaga ctgcgtgtca cgttcgcgct ggactgctgt gatcgtgagg cactgcactg
      601 ggcggtgact accggcggct tcaacagtga aacagtacag gacgtcatgc tgggagcggt
      661 ggaacgccgc ttcggcaacg atcttccgtc gtctccagtg gagtggctga cggataatgg
      721 ttcatgctac cgggctaatg aaacacgcca gttcgcccgg atgttgggac ttgaaccgaa
      781 gaacacggcg gtgcggagtc cggagagtaa cggaatagca gagagcttcg tgaaaacgat
      841 aaagcgtgac tacatcagta tcatgcccaa accagacggg ttaacggcag caaagaacct
      901 tgcagaggcg ttcgagcatt ataacgaatg gcatccgcat agtgcgctgg g
//
LOCUS       DAJVXP010000330          942 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVXP010000330 DAJVXP010000000
VERSION     DAJVXP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.337"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            192..>942
                     /locus_tag="OQS10_004313"
     CDS             192..>942
                     /locus_tag="OQS10_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HCS2295152.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQ"
BASE COUNT          207 a          291 c          246 g          198 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      781 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      841 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      901 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gc
//
LOCUS       DAJVXP010000331          926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVXP010000331 DAJVXP010000000
VERSION     DAJVXP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.338"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..926
                     /locus_tag="OQS10_004314"
                     /pseudo
     CDS             1..926
                     /locus_tag="OQS10_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT          253 a          175 c          221 g          277 t
ORIGIN      
        1 ttattgtgca gatattatgc aggttgtgaa gtatcgacat gtcaaaaata cagggtttat
       61 ctcttttgac ggtaaaacct tcgtctatta cctctatccg gtaactcaca atcgtagtct
      121 gatatttttg cttggtctgg agcgtttttc tttactgtca aaatcgctgg cgatggacag
      181 cgagaacctg atgttctctc tatttaagaa cggtaaaccg gtgatgaata taatgctaaa
      241 aacgccatct tcaccgtttc ggaagcgatg gagcacttcg cctatttgcc gaccggattg
      301 tacgtatttg cgtataaaaa agatgtttat ttgcgggtct gtacattgat tattttcttt
      361 gccgcattgg tggcagtgat atcgggtacc agttgcctct atctggtacg cagagtgatt
      421 aatcgtggta ttgtggagaa agaagccatc attaataacc attttgaacg cgtactggat
      481 ggcgggcttt tcttttcggc tgccgatgtc aaaaaactct acagtatgta taactcggcg
      541 ttcctggacg acctgaccaa agcaatgggc agaaaatcct ttgacgaaga tttaaaagcg
      601 ctgccggaaa aaggcggtta tttgtgcctg tttgacgtcg ataaattcaa aaacattaac
      661 gacaccttcg gtcatttgct gggcgatgaa gtgttgatga aagtggtgaa aatccttaaa
      721 tcacagatcc cggtagataa aggtaaaatc taccgcttcg gcggtgacga atttgcggtg
      781 atttatacgg gtggaacgct ggaagagttg ctatcgattc taaaagaaat cgttcatttc
      841 caggtgggaa gcattaattt aagtaccagt atcggtgtag cgcattcaaa tgaatgtacc
      901 accgtcgaac gcttgaaaat gctggc
//
LOCUS       DAJVXP010000332          904 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVXP010000332 DAJVXP010000000
VERSION     DAJVXP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.339"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            53..283
                     /locus_tag="OQS10_004315"
     CDS             53..283
                     /locus_tag="OQS10_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCS2295153.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(375..>904)
                     /gene="rlhA"
                     /locus_tag="OQS10_004316"
     CDS             complement(375..>904)
                     /gene="rlhA"
                     /locus_tag="OQS10_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS2295154.1"
                     /translation="PGALFVPNSLLNQFRREAADMLDAARLASYQRGSRKPVADPAPV
                     FPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPVMITKHCLRFAFNLC
                     PKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKNHILKMPLPGSVVAS
                     VSPDELLKTLPKRKG"
BASE COUNT          222 a          216 c          243 g          223 t
ORIGIN      
        1 ctcaagaaac gatcggcatc atagcggcaa aaggtaagaa aaaggtggca ggatgtttac
       61 gaaggcgtta tcggttgtct tattaacgtg tgcgctgttc tcaggacaac tcatggcagg
      121 gcacaaagga catgaatttg tgtgggtaaa gaatgtggat catcagctgc gtcatgaagc
      181 ggacagcgat gaattgcgtg ctgtggcgga agagtcggcg gaaggtttgc gcgagcattt
      241 ttactggcaa aaatcgcgca aaccagaagc gggacaacgt tgagtcgttt gatgccggat
      301 gcggcgtgac gccttacccg gcaggcaata tcatgcagat tcaatgagtt gtgagtaacc
      361 agaaaactgg cgttttaccc ttttcgctta ggcaatgttt tcagcagctc atccggactt
      421 acggatgcca ctacgcttcc cggtaacggc attttcagta tgtgattttt gattttgcca
      481 atgacgtgca tctcgcatgg gcggcaatca aactttagcg ttaatacttc atcgccgtta
      541 accagttgca ttggcgtcgc cttccagctt ttgatattgc ctttcgcctg tttcgggcac
      601 agattaaagg caaagcgcag acaatgcttg gtgatcatca ccgggacttc gcccttctct
      661 tcatgtgcct catacgccgc gtcaatcagc tgcacaccat agcgatgata aaattcacgc
      721 gctttctggt tgtatacgtt cgcgaggaaa ctcagatgcg tttgcggaaa aaccggcgca
      781 ggatcagcaa ccggtttacg gctgccgcgc tggtaactgg caagacgcgc agcatccagc
      841 atgtcagcag cttcacggcg gaactggttt aacagactgt ttggtacaaa cagcgccccc
      901 ggca
//
LOCUS       DAJVXP010000333          897 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVXP010000333 DAJVXP010000000
VERSION     DAJVXP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.340"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..643)
                     /locus_tag="OQS10_004317"
     CDS             complement(<1..643)
                     /locus_tag="OQS10_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2295155.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>897)
                     /gene="tabA"
                     /locus_tag="OQS10_004318"
     CDS             complement(766..>897)
                     /gene="tabA"
                     /locus_tag="OQS10_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS2295156.1"
                     /translation="DEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT          269 a          199 c          142 g          287 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatc
//
LOCUS       DAJVXP010000334          895 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVXP010000334 DAJVXP010000000
VERSION     DAJVXP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.341"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..643)
                     /locus_tag="OQS10_004319"
     CDS             complement(<1..643)
                     /locus_tag="OQS10_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2295157.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKP"
BASE COUNT          252 a          183 c          176 g          284 t
ORIGIN      
        1 agggcttgtc ggccagctgt tgttctatca acagtcggtt gccgctggag gagcgtacgc
       61 caatcatttt gtaacccact aactcctgcc attctacaag ctgctttttg gccaatggat
      121 ggtcacgccg acaggccagc acgaacggct cgttgaccag tggggtaaaa tcaatggatg
      181 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
      241 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
      301 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaacgtca
      361 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
      421 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
      481 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
      541 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaacgaa ctggactctg
      601 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
      661 gaagcgacga ttttgggtgg ctggccgtta aaaattttaa ctgcatttag ccaacttaaa
      721 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgcccttaat
      781 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
      841 ctgggtaatg actccaactt attgatagtg ttttatgttc agataatgcc cgatg
//
LOCUS       DAJVXP010000335          889 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVXP010000335 DAJVXP010000000
VERSION     DAJVXP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.342"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..612
                     /locus_tag="OQS10_004320"
     CDS             <1..612
                     /locus_tag="OQS10_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS2295158.1"
                     /translation="GGQIARHVINQLADKQTIKQTLFARQPAKIHKPYPTNSKIIMGD
                     VLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYDEVP
                     GKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFK
                     GTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          303 a          176 c          178 g          232 t
ORIGIN      
        1 ggcggtcaga tagcccgcca tgtgattaat caactggcag ataaacaaac cattaaacaa
       61 actttatttg cccgtcagcc agcaaaaatc cacaaaccct atccaactaa tagcaagatt
      121 attatgggag atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg acaggacgtt
      181 gtgtatgcca accttacggg ggaagattta gatattcagg caaattcagt gatcgctgcg
      241 atgaaagcct gtgatgttaa acgtctgatc tttgttcttt ccctcggtat ttatgatgaa
      301 gttccaggaa aatttggcga atggaataat gcagttattg gtgagcccct aaaaccattc
      361 cgcagggctg cggatgcaat tgaggcttct ggactggaat atacaattct tcgccctgcc
      421 tggctgactg atgaagatat tattgattat gagcttacct cacgcaatga accattcaaa
      481 ggtacgatcg tatcacgtaa gagtgtcgca gcattaatca cagatatcat cgataaacct
      541 gagaaacaca ttggtgagaa tataggtatc aatcaacccg tgactgatgg tgacaagcca
      601 ttattcatgt aaaccaatta aatagttacc atcgtaatgt actatgtgca gtacgattta
      661 ttaaacttat taaggtgaac tgatcaatcc gatgcgatta acatgtgtag aaccagtaag
      721 aataaaatac gccatgtata taacattacg acttacatct aagtcagcat aattctaata
      781 tctgcacatc aataaaaatg caaacaactt ccggcaaaaa aagtgataca agatcgatga
      841 catagcgttg aagaaaagga tttgataatc cgcaaaattt gcacaacac
//
LOCUS       DAJVXP010000336          858 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVXP010000336 DAJVXP010000000
VERSION     DAJVXP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.343"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>858
                     /locus_tag="OQS10_004321"
     CDS             <1..>858
                     /locus_tag="OQS10_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2295159.1"
                     /translation="DGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGET
                     AERWRYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETR
                     HAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFGR
                     YELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGV
                     HTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGRLT
                     EKTDLIPEGV"
BASE COUNT          199 a          286 c          253 g          120 t
ORIGIN      
        1 tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga
       61 tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt
      121 gaagggtgaa accgcagagc ggtggcggta cgacgaacgc ggctggctga cagacatcag
      181 ccatctcagc gaagggcacc gggtgacggt gcattacggg tatgatgaga aaggccggct
      241 gaccggtgag cgtcagacgg tgcatcaccc gcagacggaa gcactgctct ggcagcatga
      301 gaccagacat gcgtacaacg cgcaggggct ggcgaaccgc tgtataccgg acagcctgtc
      361 cgccgtggaa tggctgacct acggcagcgg ctggctgtca ggcatgaaac tcggcgacac
      421 accgctggtg gagtacaccc gcgaccgcct gcaccgggaa acgctgcgca gcttcggccg
      481 ttatgaactc accatcgctt atacccctgc cgggcagtta cagagccagc acctgaacag
      541 cctgctgtct gaccgcgatt acacctggaa cgacaacggc gaactcatcc gcatcagcag
      601 cccgcgccag acccggagtt acagctacag caccaccggc aggctgaccg gcgttcacac
      661 caccgcagcg aatctggata tccgcatccc gtatgccaca gacccggcgg ggaaccgcct
      721 gcccgacccg gagctgcacc cggacagcac cctcagcatg tggccggata accgtatcgc
      781 ccgtgacgcg cactatcttt accggtatga ccatcacggc agactgacag agaaaaccga
      841 cctcatcccg gaaggggt
//
LOCUS       DAJVXP010000337          851 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVXP010000337 DAJVXP010000000
VERSION     DAJVXP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.344"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..227)
                     /locus_tag="OQS10_004322"
     CDS             complement(<1..227)
                     /locus_tag="OQS10_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS2295160.1"
                     /translation="MRKAGNSPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDK
                     VFCPTRGGNKMAFVDKDGVEVESSTHWMPLP"
     gene            complement(341..577)
                     /locus_tag="OQS10_004323"
                     /pseudo
     CDS             complement(341..577)
                     /locus_tag="OQS10_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          218 a          237 c          181 g          215 t
ORIGIN      
        1 tccggtagcg gcatccagtg agttgagctc tctacttcaa cgccgtcttt gtccacaaaa
       61 gccatcttat tcccgccacg agtggggcaa aaaaccttat cccaggaacc ggggaataca
      121 ttcccgagat catcgagaag aatcacatcg caatattcag caggaactgc atcactacag
      181 cttatccagc cagccggagc taccggagag ttgccagcct tacgcatggc catttccacg
      241 atttcaacca tatctcctgg tggaatttta caaagttgcc caatacattt ctgctgcctg
      301 gcatattcga gaatgtgctc cagtttggtt cgattaatca ttatttatct cccttaagca
      361 tggcagcgcg gcaggcgttc cagccatcaa cataatcaaa cgtattacta tcgtttggcc
      421 cgatttcatc cggcactatc ggctctggtt ggatagtgac gttggcaaag gcagcacgca
      481 aaccggtttt aatttcttcg atttcatcag agccaagcga tgaatctgac agtgcgtgat
      541 ggaatgcgta agccatgtcg tcgtttactg caaccggcac tgccggcgct gcgtaaagcg
      601 gtgttatatc tgcccgaaaa tcacatgctt tatgcagtcg tacccaacac tcaacttccg
      661 ctttgtcaaa atatatagca gtgaacgtat tagattcctg gtcaacttga gtaaacgtca
      721 ccttccacgc tacagctacg gctttccatc catcaggttg tcgcattcgc ggggcagcat
      781 aaagctcatg cattccatca ggcagcgagt gacccacata ctcaccgaac ccgtcaatac
      841 acatgccccc a
//
LOCUS       DAJVXP010000338          843 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVXP010000338 DAJVXP010000000
VERSION     DAJVXP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.345"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            81..248
                     /locus_tag="OQS10_004324"
     CDS             81..248
                     /locus_tag="OQS10_004324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295161.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            356..589
                     /locus_tag="OQS10_004325"
     CDS             356..589
                     /locus_tag="OQS10_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295162.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
BASE COUNT          254 a          165 c          233 g          191 t
ORIGIN      
        1 tcatgatatg aatgcagggt ttgatgaaaa gattttgttc ccctcatcag ttgaaagcac
       61 tgcgggttaa ggagtaacac atgaccacta ttaccaaaga acgtattgaa ttgttcatta
      121 aaaacccgct tgaaaacggg cttacccgtg gtgaacaaat ggaactggca cggattgcgc
      181 tggcatcgct ggaagcagag ccggttgtgt tctggtttga aaaatatcaa gaaggggcta
      241 cggcatgacg acttttacca gagagcagtt aatagctcac gcagaggaga ctattgaagc
      301 acagagactg tgcataccgg gcacaatcga ccatgacatc atccgcacat ataagatgga
      361 tattgctgtt ctggaaatcg cactggtatc gctggcagca gagccagccg gtaaattgca
      421 tgaatacaaa ccagtgggat atcagcgtct ggtcgatgag ttaaccatgc tggtaaagca
      481 gttaacctgg caactgagga aagcgaagcc agactgcaaa ttaccggata aggcgatgag
      541 ttatctggag cggaacggac tgataagcgt ggaggatatt ttacgatgac ctggcctgaa
      601 gcattaacaa cggtaggaat tgcgatggcg gtggcgctgg tggtgtattc gatttgccgc
      661 tggggataaa aacggtttgc gggaaaagga gagttaagta gaattgcagc gggtgcttga
      721 ggctatctgt ctcaggcatg aacaccaaaa ggcagataga gaaaagcccc agttaacatt
      781 acgcgtccgg caagacgctt aacattaatc tgaggctcaa tctatgaacg gcaaatctag
      841 gtt
//
LOCUS       DAJVXP010000339          797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVXP010000339 DAJVXP010000000
VERSION     DAJVXP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.346"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..282
                     /gene="sfmF"
                     /locus_tag="OQS10_004326"
     CDS             <1..282
                     /gene="sfmF"
                     /locus_tag="OQS10_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCS2295163.1"
                     /translation="GCPPGSVAILFTGTPASDTNLLALDDPAMAQTVAIELRNSDRSR
                     LALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVEKADAIFMINYN"
     gene            complement(285..>797)
                     /gene="fimZ"
                     /locus_tag="OQS10_004327"
     CDS             complement(285..>797)
                     /gene="fimZ"
                     /locus_tag="OQS10_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCS2295164.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          229 a          160 c          171 g          237 t
ORIGIN      
        1 ggatgtcctc cgggttccgt tgcgatttta tttacgggaa cgccggcatc cgataccaac
       61 ctgctggctc tggatgatcc cgcaatggca caaaccgtcg ccatcgaatt acgtaatagc
      121 gatcgctccc ggctcgcact gggggaggcg agcccgactg aggaagtaga tgcaaatggc
      181 aatgtcacac taaacttttt tgccaattat cgagcgttag ccagcggtgt tcagccaggt
      241 gtggagaaag cggatgcgat atttatgatc aattataatt aatattatat taattcgtat
      301 aatttggcgt agtcgataag ctctacaatt gaatgcaaac ctagtttgcc ataaatatta
      361 gatttatgcg cactaactgt tttattgcta agtaataact tatcggcaat ttctttatta
      421 gataatccgc taaccagata acgtaatatg gtcacttcac gattagatag cacagtgacc
      481 gttgaactat tcgtactaca tttattgctt tttatatagt tgagcgtttc gctaggaaaa
      541 aacgtgtatc cggagaggat catctgaacg ggatgaaaaa tatcattctg atcattgcat
      601 ttactgacaa aaccgttagc accagcttgt atcgctctgc cagcataaaa gcattctgat
      661 ttcgatgata aaaataacac tttcactgtg ctctggattt gtttgatcct tttcaggaag
      721 gtaaaaccgt ctgttccggg caagtctata tccataatga ttaaatcaac aggacgggtt
      781 cggagataat cgatggt
//
LOCUS       DAJVXP010000340          792 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVXP010000340 DAJVXP010000000
VERSION     DAJVXP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.347"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>792
                     /locus_tag="OQS10_004328"
     CDS             <1..>792
                     /locus_tag="OQS10_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCS2295165.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          282 a          116 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc ca
//
LOCUS       DAJVXP010000341          789 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVXP010000341 DAJVXP010000000
VERSION     DAJVXP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.348"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            285..389
                     /locus_tag="OQS10_004329"
                     /pseudo
     CDS             285..389
                     /locus_tag="OQS10_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          262 a          139 c          155 g          233 t
ORIGIN      
        1 tatcatacag ataattactg gttaacccta tatagggcat tttaaagtgt tgtgatctta
       61 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gactcacaag
      121 acaaaacagt ttcaaccagt aaaaaaacgt aaacaccatt cgcatgaatt tcgtagttaa
      181 gatctgaagc ttgcagaatg catcggtgtt actgccggag tccgtgaact tagtctgcat
      241 gaatcaccac agctttacat ctgtcgcagt aaacagcaaa atcagcaaac ttacttaatt
      301 atattgagtg cgattacaat cgctggcgat ggtacagttg gtgtggcggt ctcagtccgg
      361 aacaatttga aaatcagaat ctcacttaaa gcggcatcca ttttacaaaa gatggccgtc
      421 tgtgtatttg taatgaatac cttctacatg tttagagtgc ataatccacg acaaggcgat
      481 atcctaaaaa ttagctagca ggcaactttg atggcataga taatgttttt agtaaaaaag
      541 agttgactga atataataca gcctcttcct acataagaag gaatgtgctc acaaaaaaaa
      601 accattagtc tgcttcctgt tcttacaata atcagtatta tgtattttat ttatatagca
      661 aaatatgctt gttacacctg tcataattag gcatggtaaa attattgact gaaccctggg
      721 ggaggaagat gcacagtgat tattataata tggtttttgg tgaaaaattg gccaatattt
      781 tatatgagg
//
LOCUS       DAJVXP010000342          775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVXP010000342 DAJVXP010000000
VERSION     DAJVXP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.349"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>775)
                     /gene="tssI"
                     /locus_tag="OQS10_004330"
     CDS             complement(<1..>775)
                     /gene="tssI"
                     /locus_tag="OQS10_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2295166.1"
                     /translation="NTRTEVSTFCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQE
                     GQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRI
                     VLTGHPQANLNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLL
                     KPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTG
                     FGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPG"
BASE COUNT          133 a          247 c          217 g          178 t
ORIGIN      
        1 ccgggtgtgc ggttttcctg gtggtaggta cgccccataa taatcggctg gtccggatcg
       61 ccgttgagga agtccacaat gacctcctga cccacgcgcg gtatcgcaag gttaccgaat
      121 ccggtgcctg cccacgcctg tgccacacgg atccagcatg aactgtcctg gtttgacggg
      181 ttatagcggt cccagttaaa tttcacccgc acgcggccat gttcatcaca gaagatttcc
      241 tcgcctgccg gtcccgtcac gacggcgctc tgcgggccat ccaccagcgg tttcagcaac
      301 ggctgtggcc gccatgtccg gtctgccggt attaccgcaa agtggttatt cagcgtggtt
      361 cctgaaccac tgcgtcccgg caccgcctgc ggctgctcgc cgtgcagttc acttgccacc
      421 acctgccatt cccggttcag gttcgcctgc ggatgccccg tcagcacaat tcgccgtccc
      481 ggccatatct ccggagaacg gcttgttccg cgcgccactt ctgcgttgtt gcgccagcca
      541 tccatctgcc agcgggcaaa gttctgcccg tgagcaccct tgaaacgtcc ggggtagtca
      601 tacacttcat actgtgtgcg ctggtaatcc tggtactggc cttcctgatc aaaacgccct
      661 gcccagccgg gtcgtttaaa ggtgtagtct ttggtcacca cggaagaagg acggatttgt
      721 gcgctgtagc ggaactggct gatgcagaag gtacttacct cggtacgggt gttcg
//
LOCUS       DAJVXP010000343          757 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVXP010000343 DAJVXP010000000
VERSION     DAJVXP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.350"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..359)
                     /locus_tag="OQS10_004331"
     CDS             complement(<1..359)
                     /locus_tag="OQS10_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS2295167.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLL"
     gene            complement(359..574)
                     /gene="essD"
                     /locus_tag="OQS10_004332"
     CDS             complement(359..574)
                     /gene="essD"
                     /locus_tag="OQS10_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCS2295168.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          206 a          188 c          171 g          192 t
ORIGIN      
        1 agaagcgtcg atgttctgaa attgccagca cccacgttgt agacgaacga gtaaagagcg
       61 cagcgcgttg tttccggtat atcgactttg atgtacgggt taatttgtct ggcgacagtg
      121 gcaaggtctt tattcaggag ggctttgcac tctgcttcgg tatacgtttt accgggaatg
      181 atgtcttttc ctgtatgccc gtaacataca gtccatacac caactatgtc tttgtaagga
      241 ttatgtctca caccttccag accatcgtta ccacctgggc cagtgattaa cacagatgct
      301 atagcaatag ccccgccacc aatagcagca gcaacagctt ttcgtaatga tggaggcatt
      361 atccacctct cgcagccttg cgcttatctt ctttaatctt gaaataaagg tttgtcaggt
      421 acgtcagcag gccaaatacc aggctaccca gcactccaat tgccgcccac tgtgagggcg
      481 tgactttatc tagcagctgt aaaaaccagt acccggcact acctgctgag gtgccatagg
      541 cgacacccgt tgttaactta tccatggatt tcataacccc acctcgcaga caaagcgggt
      601 gtaaattgag ggaatactac gaaacgtaac agactcggag tcagtgaata actcaggtat
      661 tgggttatca gctaatatcg agactcaaaa aatggaaaaa cccgctcgac ggcgggttta
      721 agctgtgtga cgaagtaacc actcttaaca gcataac
//
LOCUS       DAJVXP010000344          743 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVXP010000344 DAJVXP010000000
VERSION     DAJVXP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.351"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..396)
                     /locus_tag="OQS10_004333"
     CDS             complement(<1..396)
                     /locus_tag="OQS10_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS2295169.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAJVXP010000345          732 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVXP010000345 DAJVXP010000000
VERSION     DAJVXP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.352"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..292
                     /locus_tag="OQS10_004334"
     CDS             <1..292
                     /locus_tag="OQS10_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HCS2295170.1"
                     /translation="NNISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQM
                     HEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            476..577
                     /locus_tag="OQS10_004335"
                     /pseudo
     CDS             476..577
                     /locus_tag="OQS10_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          206 a          136 c          180 g          210 t
ORIGIN      
        1 gaataacatc agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac
       61 ggcaatcgat aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc
      121 ggaagtgcag atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga
      181 taacgtgaag acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac
      241 caatgtgttg aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg
      301 gtttggtcgt tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta
      361 tgtttgtgcg cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc
      421 tggtagtcgt tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac
      481 gaatatactg ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc
      541 aaacgctgat gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg
      601 tcatctgaaa ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca
      661 atgtgaatct cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat
      721 aatatctaaa ct
//
LOCUS       DAJVXP010000346          731 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVXP010000346 DAJVXP010000000
VERSION     DAJVXP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.353"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>731)
                     /locus_tag="OQS10_004336"
     CDS             complement(<1..>731)
                     /locus_tag="OQS10_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS2295171.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAJVXP010000347          730 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVXP010000347 DAJVXP010000000
VERSION     DAJVXP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.354"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..465
                     /gene="pagP"
                     /locus_tag="OQS10_004337"
     CDS             <1..465
                     /gene="pagP"
                     /locus_tag="OQS10_004337"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS2295172.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            640..>730
                     /gene="cspE"
                     /locus_tag="OQS10_004338"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             640..>730
                     /gene="cspE"
                     /locus_tag="OQS10_004338"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS2295173.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVF"
BASE COUNT          210 a          148 c          172 g          200 t
ORIGIN      
        1 acgtttagag aaaatattgc acaaacctgg caacagcctg aacattatga tttatatatt
       61 cctgccatca cctggcatgc acgtttcgct tacgacaaag aaaaaaccga tcgctataac
      121 gagcgaccgt ggggtggcgg ttttggtcag tcgcgatggg atgaaaaagg aaactggcat
      181 ggcctgtatg ccatggcatt taaggactcg tggaacaaat gggaaccgat tgccggatac
      241 ggatgggaaa gtacctggcg accgctggcg gatgaaaatt ttcatttagg tctgggattc
      301 accgctggcg taacggcacg cgataactgg aattacatcc ctctcccggt tctactgcca
      361 ttggcctccg tgggttatgg cccagtgact tttcagatga cctacattcc gggtacctac
      421 aacaatggca atgtgtactt tgcctggatg cgctttcagt tttgagacaa atgaagtttt
      481 agtaacttct ttaaaatcaa taactaaaat aagtaacatc aaaaataacg cgacttttat
      541 cactttttag taaagttgca ctggacaaag cgtaccacaa ttggtgtact ggtaaccgac
      601 acagcatttg tgtctatttt tcatgtaaag gtaattttga tgtctaagat taaaggtaac
      661 gttaagtggt ttaatgagtc caaaggattc ggtttcatta ctccggaaga cggcagcaaa
      721 gacgtgttcg
//
LOCUS       DAJVXP010000348          729 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVXP010000348 DAJVXP010000000
VERSION     DAJVXP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.355"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>729
                     /locus_tag="OQS10_004339"
     CDS             <1..>729
                     /locus_tag="OQS10_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295174.1"
                     /translation="CRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWL
                     KDAPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFL
                     PKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVA
                     KLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANI
                     RRDYLHKVTTTVSKNHAMIVIEDL"
BASE COUNT          214 a          177 c          179 g          159 t
ORIGIN      
        1 atgtcgtttc gttttcaatc gtgctctggc acgtcagaat gagaatcatg aggccggtaa
       61 taaatacatc ccttacggga aaatggcttc ctggctggtt gagtggaaaa atgccactga
      121 aacgcaatgg cttaaagatg ccccgtcaca gccattgcaa cagtcactga aagaccttga
      181 gcgggcttac aaaaacttct tccagaatcg ggctgctttt ccccgattca aaaagcgggg
      241 acagaatgat gtattccgct acccgcaggg cgttaagctc gatcaggaaa acagccgtat
      301 ttttctgccg aaactgggct ggatgcgcta ccggaacagc cgtcaggtca cgggtgttgt
      361 gaaaaatgtc actgtcagcc agtcctgcgg taagtggtac atcagtattc agacagaaag
      421 tgaagtatca actccggttc acccttcagc atcaatggtc gggctggatg ctggcgtggc
      481 taaactcgcc acgctgtcag atggcacagt ctttgagcct gtaaacagtt ttcagaaaaa
      541 ccagaagaag ctggcgagac ttcagcgaca gttaagccgc aaggtcaaat tcagcaacaa
      601 ctggcagaag cagaaacgca aaatacagcg actgcattcc tgtatcgcaa atatccgcag
      661 ggactacctt cacaaagtca caacgaccgt cagcaaaaac cacgcaatga tagtcattga
      721 ggatttgaa
//
LOCUS       DAJVXP010000349          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVXP010000349 DAJVXP010000000
VERSION     DAJVXP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.356"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            35..726
                     /locus_tag="OQS10_004340"
                     /pseudo
     CDS             35..726
                     /locus_tag="OQS10_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJVXP010000350          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVXP010000350 DAJVXP010000000
VERSION     DAJVXP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.357"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>726
                     /locus_tag="OQS10_004341"
     rRNA            <1..>726
                     /locus_tag="OQS10_004341"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          206 a          159 c          223 g          138 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagct
//
LOCUS       DAJVXP010000351          717 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVXP010000351 DAJVXP010000000
VERSION     DAJVXP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.358"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>717)
                     /locus_tag="OQS10_004342"
     CDS             complement(<1..>717)
                     /locus_tag="OQS10_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HCS2295175.1"
                     /translation="GSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVFN
                     AGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTN
                     ALKGDGLMRVQLSSYDKMFGFTHATGTEFAGVAQLKDRTFTLERDNTAALTHAMLQSD
                     SENTTSVNVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRN
                     YQVNGTGDVLIDVPKPWNDP"
BASE COUNT          163 a          199 c          171 g          184 t
ORIGIN      
        1 tgggatcatt ccacggttta ggcacgtcga taagcacgtc gcccgtcccg tttacctgat
       61 agttacggcc tttccaggtg tagtcacccg cgccgacaac cagcgtatcg acgctgatat
      121 atccctctgc aagcgtcgca gcaggaatat ccgtatcgaa aatgagcgta ccgccattca
      181 tggccagtcc accaatggat tgctctccca cgtttaccga tgtggtattt tcactgtcag
      241 actgcaacat cgcgtgagta agcgcagcgg tgttgtcgcg ttccagagtg aaggttctgt
      301 ctttcagttg ggcaacaccg gcgaattcag tccctgttgc atgggtaaag ccaaacatct
      361 tgtcgtagga tgacagctgc actcgcatca agccatcgcc tttgagcgca ttggtcagcg
      421 tgtaatctcc tgcactgttc gttgatgcga gaatgtcgag cgtaccgggg cttgcaatgg
      481 tgacttcact cgaacccatg cccgttaccc catctgccgt atgagacgca atggtcagga
      541 ggccgtcatt gaccagcgtt ttaccgctat aagtattccc tgcattgaac accgtatggc
      601 ctgcatcatg gatcactttt ccgtctttat cgtcacctgt aatcagcata tcgacctggt
      661 agccggcatc actgttatta gtatgattaa agacaaaaac accttcgcca gaaccaa
//
LOCUS       DAJVXP010000352          710 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVXP010000352 DAJVXP010000000
VERSION     DAJVXP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.359"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(261..>710)
                     /locus_tag="OQS10_004343"
     CDS             complement(261..>710)
                     /locus_tag="OQS10_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS2295176.1"
                     /translation="HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQ
                     KKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAE
                     LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          183 a          169 c          114 g          244 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttcag cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag
      301 tttctgtcca tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca
      361 ttcgtcaaaa tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct
      421 cttcttattt tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc
      481 tccacgtgcc agtggtttcg gattgctgtg gcgaactttt ctgcagttaa atcagcagaa
      541 ctgatataat atctgaccgt catttctggc tctttctttt gttctgctat tattgaccga
      601 aaggagactg ccacgcataa tttcttcagt cctttccatt caaacgtgaa atcaataagt
      661 tcatcaggga catcgcaaac aatatgaaga cggatttctt ctctgccgtg
//
LOCUS       DAJVXP010000353          710 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVXP010000353 DAJVXP010000000
VERSION     DAJVXP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.360"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..305)
                     /locus_tag="OQS10_004344"
     CDS             complement(<1..305)
                     /locus_tag="OQS10_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS2295177.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(344..631)
                     /locus_tag="OQS10_004345"
     CDS             complement(344..631)
                     /locus_tag="OQS10_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295178.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
BASE COUNT          192 a          188 c          145 g          185 t
ORIGIN      
        1 tgctctgcgg cttcgctcag agctggcgta tgttcccggc ctactgattc acacaggtaa
       61 tcaacgaaac cggacaccag ttttcgttcg cgaggcgtga tcgccccacc gaccggagtc
      121 cagtaatcga aacccagttg caggagtttg aaaaaacgct tgtggaatgc gtagttacac
      181 acacgcttaa agtctgcgtg tatccactca cctattttga tttgatgcag aaaatcgcaa
      241 ctctccggcg tcgccgggag aagtaatccg gaagaggttt gtttgaccag ttgtatatgt
      301 gccatcggct ttctccggtg gcacggtgtt acacagcagg agttcaatcc tgctcaagat
      361 tgtagatgag tttattcttc agcaaaagca gaaaaccagc cttaaaacca atctctttca
      421 aaacccgtaa tgatgtgaca aattcgtcct cacgcaaaat aaaaccgtcc gtcagaagtc
      481 cattacaaaa ataaaataac acagcaccgc tcttcctttg ttgagattgc aaacatctaa
      541 tgcggcaatg gctgacaatc gctccattct caacgcgcac agcatagagg ccattttcac
      601 taaaaatttc acgcaattct tcgattttca tcttcagaat ccttccagat aaatagctct
      661 cccctgttcg gggtccatcc ctcttctccc tgcgcgctac ttaagtgagt
//
LOCUS       DAJVXP010000354          700 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVXP010000354 DAJVXP010000000
VERSION     DAJVXP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.361"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>700
                     /locus_tag="OQS10_004346"
     CDS             <1..>700
                     /locus_tag="OQS10_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2295179.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEM"
BASE COUNT          134 a          203 c          205 g          158 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
//
LOCUS       DAJVXP010000355          680 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVXP010000355 DAJVXP010000000
VERSION     DAJVXP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.362"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>680
                     /gene="cadA"
                     /locus_tag="OQS10_004347"
     CDS             <1..>680
                     /gene="cadA"
                     /locus_tag="OQS10_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCS2295180.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNL"
BASE COUNT          185 a          174 c          167 g          154 t
ORIGIN      
        1 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
       61 gtgatttacg aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg
      121 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
      181 accacttctc ctcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
      241 ggcaatgcag gtaaacgttt gatcaacggt tccattgaac gtgcgatcaa attccgtaaa
      301 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
      361 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
      421 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
      481 atggaaaaag acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac
      541 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
      601 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
      661 cgtgcgttcg acctgaacct
//
LOCUS       DAJVXP010000356          678 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVXP010000356 DAJVXP010000000
VERSION     DAJVXP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.363"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..369)
                     /locus_tag="OQS10_004348"
                     /pseudo
     CDS             complement(1..369)
                     /locus_tag="OQS10_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352717.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          153 a          190 c          188 g          147 t
ORIGIN      
        1 aggcataccg gaaaggtatc ctgccagcac atcaaggcgc ttttcactgt agggggtgag
       61 ataacaacct gtaacggcat ctgccagcga gggcaattcc ggcgtgttaa tgtcctgcgg
      121 gcattcgcgt cacatttttg ccagtgcagg agcgtgccag ccaccgcaga cgactaacac
      181 gtcgccattg ttttgctgca tcgcccatcc aatccagcgg gccataaagg cttcgcgctg
      241 acgattgagt gcatcgccgg gggaatcgcc ccgcagttgg gcaaaatagt gcgccagtgc
      301 agagggtaat gcagtttgct ggctttcatc ttcgaacaag tgatcccaca gggtgtcgct
      361 gttatccatg cgggtggcac gcagcagcaa ggcctggctt tcctcttgcg tatcaggcga
      421 atcgtccact tcttcactct gcgcccagca aggcaaatcg atgaagtaag tttgtgcctg
      481 aatacgacgt gcggcttgta gcgcctgcca ctccggcgaa aattcagcaa atggcgtcca
      541 ggcaccacgc ccgggggctg caccgtcctg atactggcaa taactgtaaa tagctaccgg
      601 aagctggtgg gctaaaaaca gttcgtctac ccggtcatta aaatcagccg ggccttcaat
      661 caatacgtat cgtggccg
//
LOCUS       DAJVXP010000357          677 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVXP010000357 DAJVXP010000000
VERSION     DAJVXP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.364"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>677)
                     /gene="ydcO"
                     /locus_tag="OQS10_004349"
     CDS             complement(<1..>677)
                     /gene="ydcO"
                     /locus_tag="OQS10_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCS2295181.1"
                     /translation="ASSAAIIWQAAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYR
                     VPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSL
                     AAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVI
                     AQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYS
                     APVSPLI"
BASE COUNT          190 a          181 c          177 g          129 t
ORIGIN      
        1 caattaatgg cgaaacagga gccgaatatc cagctgcttt cattgctgcg atacccggtg
       61 cgttttgcga tgccatcgtc accagaaaaa gggggagtgc aacgctcagg ctgtgagcaa
      121 acgaaaaatc aggggtaata taagtgggga gaacgggttt aaagacaaca tcagttgtga
      181 caacgtcacc ttgcgcgata acgatcacga tcccaataat catcgcggca attaccgcat
      241 agcgcggcgc aacggccttg gttgccagcc ataccagcaa catacttcca cacaacgtaa
      301 attgaccgtc cagactggca aacgcctgta aaccaaagcg taataaaatc ccggcaagca
      361 ttgccgccgc aagcgagtgc ggaataatgc gcatcagacg agcaaagagt cccgttatgc
      421 cgcagaggac tattaacgcg ttggtgacaa taaaaacgcc gatggcttcg ttaagtgtta
      481 gtccctgcaa tccggtgacc aacaaagccg cgccaggcgt tgaccatgcg gtgagaacag
      541 gtacgcgata ccataatgtc agagtcagcg tactgacgcc cattgccagc cccagcgccg
      601 tcatccagcc agagatttgt gcagtggtgg ctccggcgac aatcgctgct tgccagatta
      661 ttgccgctga actggcg
//
LOCUS       DAJVXP010000358          674 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVXP010000358 DAJVXP010000000
VERSION     DAJVXP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.365"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..480
                     /locus_tag="OQS10_004350"
     CDS             <1..480
                     /locus_tag="OQS10_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS2295182.1"
                     /translation="CSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          197 a          138 c          144 g          195 t
ORIGIN      
        1 tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag taccaaagct
       61 gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca gtttaaagat
      121 gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg ctttattccc
      181 tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc attaagcgcc
      241 gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc atctgttctg
      301 atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag tgccggcgca
      361 agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat agctgcattc
      421 ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt gctattttaa
      481 atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc taagtagaaa
      541 atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat tgagaatgat
      601 gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct tctctgacat
      661 tttatcggag aagc
//
LOCUS       DAJVXP010000359          671 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVXP010000359 DAJVXP010000000
VERSION     DAJVXP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.366"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>671
                     /locus_tag="OQS10_004351"
     CDS             <1..>671
                     /locus_tag="OQS10_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS2295183.1"
                     /translation="NQVHVVKDFNLEIADKEFIVFVGPSGCGKSTTLRMIAGLEEISG
                     GDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLKMQKIAKEVIDERVN
                     WAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDEPLSNLDAKLRVQMR
                     AEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGAPKTVYNQPANMFVA
                     GFIGSS"
BASE COUNT          165 a          158 c          204 g          144 t
ORIGIN      
        1 aaccaggtgc atgtggtgaa ggacttcaac ctggaaattg ccgataaaga gttcatcgtg
       61 tttgtcggtc cgtcaggctg cggtaagtcg accaccctgc gcatgattgc cgggcttgag
      121 gagatcagcg gcggcgatct gttgatcgac ggcaaacgaa tgaatgacgt tccagccaaa
      181 gcacgcaata tagcgatggt gttccagaac tacgcgttgt atccgcatat gacggtttac
      241 gacaacatgg cgtttggcct gaagatgcaa aaaatcgcca aagaggtgat tgatgagcgg
      301 gtgaactggg cggcgcaaat tctcggcctg cgtgagtacc tgaaacgcaa gccgggcgcg
      361 ctttccggcg ggcaacgcca gcgtgtggcg ctcgggcggg cgattgtgcg cgaagcgggc
      421 gtgtttttaa tggatgaacc gctctctaac cttgatgcca agctgcgcgt gcaaatgcgc
      481 gcagagatca gcaagctgca ccagaagctg aataccacca tgatctacgt gacccacgat
      541 cagaccgaag cgatgaccat ggcgacgcgg attgtgatta tgaaggatgg gattgttcag
      601 caagttggtg cgccgaaaac ggtttataac caaccagcga atatgtttgt tgccggattt
      661 attggatcat c
//
LOCUS       DAJVXP010000360          669 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVXP010000360 DAJVXP010000000
VERSION     DAJVXP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.367"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>669
                     /locus_tag="OQS10_004352"
     CDS             <1..>669
                     /locus_tag="OQS10_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS2295184.1"
                     /translation="TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNR
                     GYGVLVNHPQCVSFEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPA
                     LPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEW
                     DPLTFPDPEGMIRRLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWD
                     KWQPG"
BASE COUNT          159 a          168 c          181 g          161 t
ORIGIN      
        1 aacagtttac ggtctgggag agcgctttac tgccctggtg cgcaatggcc agacggtaga
       61 gacctggaac cgggacggcg gcacaagtac tgaacaggcg tataaaaata tcccgttcta
      121 catgactaac cgtggttatg gggtactggt caatcatccc cagtgtgtct cttttgaagt
      181 gggatcggag aaagtctcca aagtgcagtt cagcgttgag agtgaatatc tcgaatactt
      241 tgttatcgac ggcccgacgc cgaaagcggt acttgatcgt tatacccgct ttactggtcg
      301 tccggcgctg ccgcccgcgt ggtccttcgg cctgtggcta accacttcat ttaccaccaa
      361 ctacgacgaa gcgacggtaa acagctttat cgatggtatg gcggaacgca atctgccgct
      421 gcatgttttc cactttgact gtttctggat gaaagccttc cagtggtgcg attttgagtg
      481 ggacccgctg actttccctg acccggaagg gatgatccgc cgcctgaaag cgaaagggct
      541 gaaaatctgc gtctggatta acccctatat cggtcaaaaa tcccccgtct ttaaagagtt
      601 acaagagaaa ggctatttac tcaaacgccc ggacggttcg ctatggcagt gggataaatg
      661 gcagccagg
//
LOCUS       DAJVXP010000361          667 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVXP010000361 DAJVXP010000000
VERSION     DAJVXP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.368"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..642)
                     /locus_tag="OQS10_004353"
     CDS             complement(<1..642)
                     /locus_tag="OQS10_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS2295185.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
BASE COUNT          209 a          148 c          115 g          195 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagca
//
LOCUS       DAJVXP010000362          647 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVXP010000362 DAJVXP010000000
VERSION     DAJVXP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.369"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            15..581
                     /locus_tag="OQS10_004354"
     CDS             15..581
                     /locus_tag="OQS10_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS2295186.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
BASE COUNT          132 a          164 c          163 g          188 t
ORIGIN      
        1 tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg
       61 ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg
      121 tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg
      181 gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc
      241 cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct ggacatctcg
      301 cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg
      361 gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg
      421 cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt
      481 ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga
      541 agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc
      601 ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggtt
//
LOCUS       DAJVXP010000363          627 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVXP010000363 DAJVXP010000000
VERSION     DAJVXP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.370"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..229
                     /locus_tag="OQS10_004355"
     CDS             <1..229
                     /locus_tag="OQS10_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS2295187.1"
                     /translation="LSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQF
                     DEINIVRGQPVSGGRLGVKIYPIMHEEQVKK"
     gene            226..>627
                     /locus_tag="OQS10_004356"
     CDS             226..>627
                     /locus_tag="OQS10_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS2295188.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          176 a          132 c          177 g          142 t
ORIGIN      
        1 cctgtccgga agactggcga taaagattat tgcagagcca ccggataagc gccgtcgtga
       61 cctggacaat atcctgaaag caccactgga tgcactgaca catgcgggag tgttaatgga
      121 cgatgagcag tttgatgaaa tcaatatcgt tcgtggtcag ccagtatctg gtggacgtct
      181 gggggtgaag atttacccca taatgcatga agagcaggtc aaaaaatgaa actggaagat
      241 ttaccgaaat actactcccc aaaatcccct tgcctgaccg atgcatcggc ctcaacgtca
      301 aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct
      361 gagatggggt tttctgcgtt cctggggaaa atgggcatca gtatgaatga cagggcgcgg
      421 gcaacagaat tactggcaga ttatgcactc agtcggtgcg atcgtgtggc ggcgttgaga
      481 aaacttccgg cagaaataaa accggtagtg atgcgcatta tggcttcgta cgcttttgag
      541 gattatgccc gcagcgcagc gagtaaaaag cagtgccctt gttgctatgg ggaaaaattt
      601 attgaaagcg tagtttttac aaacaag
//
LOCUS       DAJVXP010000364          625 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVXP010000364 DAJVXP010000000
VERSION     DAJVXP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.371"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(86..433)
                     /gene="tnpB"
                     /locus_tag="OQS10_004357"
     CDS             complement(86..433)
                     /gene="tnpB"
                     /locus_tag="OQS10_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS2295189.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          140 a          167 c          179 g          139 t
ORIGIN      
        1 cagcaggaag atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat
       61 tctgtgacaa ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag
      121 gctgtcgcca gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt
      181 tgccatcacg ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc
      241 acagtccgtc actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa
      301 tgaaaacatg gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc
      361 cgttgaagcc atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag
      421 gtaacgggat catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt
      481 gccattgaag cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt
      541 cgggtcctct gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag
      601 ctgggggctc tctgaagaag gtaat
//
LOCUS       DAJVXP010000365          613 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVXP010000365 DAJVXP010000000
VERSION     DAJVXP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.372"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..419)
                     /locus_tag="OQS10_004358"
     CDS             complement(<1..419)
                     /locus_tag="OQS10_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS2295190.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYV"
     gene            complement(429..>613)
                     /locus_tag="OQS10_004359"
     CDS             complement(429..>613)
                     /locus_tag="OQS10_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295191.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
BASE COUNT          160 a          153 c          140 g          160 t
ORIGIN      
        1 acgtagccat tatttttcac ctgactaccg gtaatacgtt cgccgttgcg cagaaaatcc
       61 agtgaccaga actcaccttt gctgacacgc gcgcttaagt tgccactttt aaactcagca
      121 taacgttctg tgttttcgat tgtgaccttc acgtcctgca aaatattgag cggataatga
      181 ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca caccgacaat accttcctgt
      241 ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct gccaggtacg ttcacgcaca
      301 tcacgggggg cagcatagac caccatttca ttatcctgct gttcaacctc gaacacctga
      361 agcgggtgaa tcaaattgag gccaggttga atcaaccagt ttccatcgct aattttcatt
      421 gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg gcgtttaccc
      481 tgagccagct gttccataac ggttttcaga ttgtgcgtcg tgagtgagta gtagcgttta
      541 gcgataatcg cgctcagcaa ataacagatc gccggaacaa tcgtgaatag cgcaataatg
      601 atgctaatcg tgg
//
LOCUS       DAJVXP010000366          594 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVXP010000366 DAJVXP010000000
VERSION     DAJVXP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.373"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..285)
                     /locus_tag="OQS10_004360"
     CDS             complement(<1..285)
                     /locus_tag="OQS10_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS2295192.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLAS"
     gene            complement(418..>594)
                     /locus_tag="OQS10_004361"
     CDS             complement(418..>594)
                     /locus_tag="OQS10_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS2295193.1"
                     /translation="VVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRI
                     ALRKYQHYQRQTCT"
BASE COUNT          151 a          155 c          132 g          156 t
ORIGIN      
        1 cgacgccagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
       61 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
      121 actgaacggc ggataagttc cttccagccc taccagcagc gtgccgcgct ctttgacttt
      181 attaagcaga ccttcatctg caaaactttt aacgctcata cccgcaacca gcgccacggc
      241 catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt
      301 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
      361 tcttattctt atttaatata tatcagaagc aggcagactg gaggatttct gcacaagtca
      421 ggtacacgtt tgacgctgat agtgttgata cttccgtaac gcaatgcggt aattgttggt
      481 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc
      541 gagggaaata ttttgctcgt gtagatgatt acccaaacgg cgcagacgaa cgac
//
LOCUS       DAJVXP010000367          593 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVXP010000367 DAJVXP010000000
VERSION     DAJVXP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.374"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          197 a           85 c          136 g          175 t
ORIGIN      
        1 aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg
       61 gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa
      121 gtgatagggc agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt
      181 ctctattctc ccgtgatatt tatcatcctg cattggggga aatagatgta caagcgccat
      241 tttgctgtac aaataaccta caaaaagccc aacgaaatta attgcaaata aacgaagatt
      301 gctggaaatt atgctgatgt taattatttg tgaaatagtt gacaagagtt atagtttttc
      361 tgtggtagca cagaataatg aaagtgtgta aagaagggta aaaaaaaccg gatgcgaggc
      421 atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat aaataaagtt
      481 aatgatgata gcgggagata ttctagttgc gagtgaaggt tttgttttga cattcagtgc
      541 tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg ctt
//
LOCUS       DAJVXP010000368          590 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVXP010000368 DAJVXP010000000
VERSION     DAJVXP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.375"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            286..>590
                     /gene="mcbR"
                     /locus_tag="OQS10_004362"
     CDS             286..>590
                     /gene="mcbR"
                     /locus_tag="OQS10_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS2295194.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          120 c          147 g          151 t
ORIGIN      
        1 tttagagaat gcgccacaga cgtttatcgg cctgctgaag ggtaaaaact tcggcaaagt
       61 ggtgatccgc gtcgcgggtg atgattaaag tttcaacggc ggcgtaagcc gccataaaaa
      121 tccaggcaaa aagcttattt tcagctttaa ttaactaacc acgtcattgt tctgctgcta
      181 ataacaacaa aggtgggtgg tttatgatta ttcactttaa tacaccaggt gaactccttc
      241 tgccgtgcag gcagggttgg acagaaaaca cgcgaaggaa ttaaaatgcc tggaacggga
      301 aaaatgaaac atgtcagttt gactctgcag gttgagaacg acctgaaaca tcagcttagt
      361 attggcgcat taaaacctgg cgcacgcctg attactaaaa atctggcgga gcaattaggt
      421 atgagtatta cacctgtgcg tgaagcatta ttacgtctgg tttcggtgaa tgcgctttct
      481 gtcgcacctg cacaagcatt tacagttccg gaagtgggga aacgtcaatt agacgaaatc
      541 aatcggatcc gctacgaact ggaattaatg gcagttgctc tggctgttga
//
LOCUS       DAJVXP010000369          581 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVXP010000369 DAJVXP010000000
VERSION     DAJVXP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.376"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..456)
                     /locus_tag="OQS10_004363"
     CDS             complement(<1..456)
                     /locus_tag="OQS10_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS2295195.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVE"
BASE COUNT          135 a          149 c          150 g          147 t
ORIGIN      
        1 ctccacttgc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc tgtagtgggt
       61 ggtggagata aacggtacgg caatgcgttt gccaatcaaa tcttcgggtt tgctgatagt
      121 tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat
      181 cggcacctgt tggctggctg caaccgctaa tgggctggaa ccgaggttgc cgatttgcac
      241 gtcgccagaa gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac
      301 gctcgccccg ctctctttag caaaggtgtt gtcggcctga gccactttcg ccggttcggc
      361 tgaggtttga tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag
      421 tgcggcaaga agtgtgttac gcgatgaaat tgccatgatt gtctgctccc ctgtcttgtt
      481 atgggagcag tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgcacaga
      541 tgattttatt cttagcaaaa aaacggcaaa ggaaagatta a
//
LOCUS       DAJVXP010000370          578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVXP010000370 DAJVXP010000000
VERSION     DAJVXP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.377"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          173 a          139 c          119 g          147 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcctg atctctgtcc cggtatttta gcccgaggta
      301 tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca ttcgctaaca
      361 gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg ttcataagcg
      421 tattatcagg attgtctgga aaattcccga tactttggac tattactcct gaacaattcg
      481 aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag gtaagcgtaa
      541 accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAJVXP010000371          576 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVXP010000371 DAJVXP010000000
VERSION     DAJVXP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.378"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..71
                     /gene="csgB"
                     /locus_tag="OQS10_004364"
     CDS             <1..71
                     /gene="csgB"
                     /locus_tag="OQS10_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS2295196.1"
                     /translation="GTQKTAIVVQRQSQMAIRVTQR"
     gene            112..564
                     /gene="csgA"
                     /locus_tag="OQS10_004365"
     CDS             112..564
                     /gene="csgA"
                     /locus_tag="OQS10_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCS2295197.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          148 a          133 c          146 g          149 t
ORIGIN      
        1 atggtactca aaaaacggca attgtagtgc agagacagtc gcaaatggct attcgcgtga
       61 cacaacgtta atttccattc gacttttaaa tcaatccgat gggggtttta catgaaactt
      121 ttaaaagtag cagcaattgc agcaatcgta ttctccggta gcgctctggc aggtgttgtt
      181 cctcagtacg gcggcggtaa ccacggtggt ggcggtaata atagcggccc aaattctgag
      241 ctgaacattt accagtacgg tggcggtaac tctgcacttg ttctgcaaac tgatgcccgt
      301 aactctgact tgactattac ccagcatggc ggcggtaatg gtgcagatgt tggtcagggc
      361 tcggatgaca gctcaatcga tctgacccaa cgtggcttcg gtaacagcgc tactcttgat
      421 cagtggaacg gcaaaaattc tgaaatgacg gttaaacagt tcggtggtgg caacggtgct
      481 gcagttgacc agactgcatc taactcctcc gtcaacgtga ctcaggttgg ctttggtaac
      541 aacgcgaccg ctcatcagta ctaatacatc atttgt
//
LOCUS       DAJVXP010000372          570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVXP010000372 DAJVXP010000000
VERSION     DAJVXP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.379"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(109..282)
                     /gene="ortT"
                     /locus_tag="OQS10_004366"
     CDS             complement(109..282)
                     /gene="ortT"
                     /locus_tag="OQS10_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS2295198.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            477..>570
                     /gene="yncL"
                     /locus_tag="OQS10_004367"
     CDS             477..>570
                     /gene="yncL"
                     /locus_tag="OQS10_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS2295199.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          178 a          114 c          117 g          161 t
ORIGIN      
        1 atgacttcat ccagatgcga catactttct ccttaaccac ttgtgaaatt tatcgattca
       61 tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt aaaacagtga
      121 aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa aggcgcttaa
      181 gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc
      241 cataaccgca taaaaaacaa gcatatgttg atagagagac atgtacgggc gtccagccag
      301 atgaataaaa cgcgcgcaaa tatgcgtcat tttttgacgt gcatcaagtt ttttcaccag
      361 tttgagaaaa aatcatcaaa cagcaacttg cccttatcca tcttcagaat gagtattatt
      421 gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg gcaaaaatga
      481 atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt ttgtttactc
      541 ttatctctat gagatttggc tggtttattt
//
LOCUS       DAJVXP010000373          559 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVXP010000373 DAJVXP010000000
VERSION     DAJVXP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.380"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            109..>559
                     /locus_tag="OQS10_004368"
     CDS             109..>559
                     /locus_tag="OQS10_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCS2295200.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          135 a          132 c          149 g          143 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc
      301 tgtggttatg gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc
      361 ccctcactta tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc
      421 agacttgtgc gttcactgcg tgcaggagat ctttctgctg tctacgtacc cggcattgaa
      481 gatgaggcat tccgggattt ggcccgggca tgggcatctg cccgcgatga tttacggcat
      541 gcaaggcaac gtctgaaat
//
LOCUS       DAJVXP010000374          557 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVXP010000374 DAJVXP010000000
VERSION     DAJVXP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.381"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>557
                     /locus_tag="OQS10_004369"
     CDS             <1..>557
                     /locus_tag="OQS10_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2295201.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJVXP010000375          554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVXP010000375 DAJVXP010000000
VERSION     DAJVXP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.382"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>554
                     /locus_tag="OQS10_004370"
     CDS             <1..>554
                     /locus_tag="OQS10_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295202.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSL"
BASE COUNT          186 a          118 c          138 g          112 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tcca
//
LOCUS       DAJVXP010000376          554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVXP010000376 DAJVXP010000000
VERSION     DAJVXP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.383"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..442
                     /gene="mhpC"
                     /locus_tag="OQS10_004371"
     CDS             <1..442
                     /gene="mhpC"
                     /locus_tag="OQS10_004371"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCS2295203.1"
                     /translation="GMSLFTPMPTEGIKRLNQLYRQPTIENLKLMMDIFVFDTSDLTD
                     ALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPRLAEIKAQTLIVWGRNDRFVPM
                     DAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLVLNFLARP"
     gene            452..>554
                     /locus_tag="OQS10_004372"
     CDS             452..>554
                     /locus_tag="OQS10_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCS2295204.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDH"
BASE COUNT          121 a          146 c          155 g          132 t
ORIGIN      
        1 cggcatgagt ttgtttacgc cgatgccaac cgaaggtatt aagcgactga atcagcttta
       61 tcgtcagccg actatcgaaa acctgaagct gatgatggat atcttcgttt tcgataccag
      121 cgatttgacc gacgccctgt ttgaagcgcg cctgaataat atgctgtcgc gccgcgatca
      181 cctggaaaac ttcgttaaga gcctggaagc taatccgaaa cagttcccgg attttggccc
      241 acgtctggcg gaaatcaaag cgcaaaccct gattgtctgg gggcgcaacg accgctttgt
      301 gccgatggat gcgggtctgc gtctgctgtc cggcattgcc ggttctgaac tgcatatctt
      361 ccgcgactgt ggtcactggg cgcagtggga acatgccgac gctttcaatc aattggtgct
      421 gaatttcctc gcacgccctt aaggaatggt catgacgaag catactcttg agcaactggc
      481 ggcggattta cgccgcgccg cagagcaggg cgaagcgatt gcgccgctgc gcgatctgat
      541 tggtatcgat cacg
//
LOCUS       DAJVXP010000377          552 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVXP010000377 DAJVXP010000000
VERSION     DAJVXP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.384"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..540)
                     /locus_tag="OQS10_004373"
                     /pseudo
     CDS             complement(1..540)
                     /locus_tag="OQS10_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          200 a          119 c           71 g          162 t
ORIGIN      
        1 tactgaatga taccccctct tctttcaatt tcatctttag taaactttcg ctgagatata
       61 atcttgatga tatatcctta agacgccatg gtgcagataa atccgtatga ataatcgttt
      121 taactttagc cctgtgcttt tcaaacatcc aaacagaaat actccaaatt gtttttcaga
      181 agaaaatgca gagagacatg taaaagcaat agactcatca aaaatttcta tgtattcagc
      241 acagtgatta gcccaactaa ttaatccttt aattaaactg aagtcagcaa agtttatttt
      301 taaataagat agatattccc tacaatcaga aatatccctt aagttattag ctttcaaata
      361 gcgactaata aactcagcgc caaaatccgc cactatcact ctttcaggat atgtcaggaa
      421 aaaatctcta gagctagagt cgacaagtac agatgaacct ctctccagaa acacactctc
      481 ttttccgaaa taaacatcaa aggactccaa aatcaatata actgaacatg tagttgccat
      541 atcatccacc ca
//
LOCUS       DAJVXP010000378          550 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVXP010000378 DAJVXP010000000
VERSION     DAJVXP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.385"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..550
                     /locus_tag="OQS10_004374"
                     /pseudo
     CDS             1..550
                     /locus_tag="OQS10_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          195 a          121 c           84 g          150 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggccatg
//
LOCUS       DAJVXP010000379          546 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVXP010000379 DAJVXP010000000
VERSION     DAJVXP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.386"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>546)
                     /locus_tag="OQS10_004375"
     CDS             complement(<1..>546)
                     /locus_tag="OQS10_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS2295205.1"
                     /translation="SLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVM
                     RDGTIALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTSVLTL
                     ENLTGEGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINK
                     LSTGERLLRGLVYLPEDRQSS"
BASE COUNT          123 a          152 c          144 g          127 t
ORIGIN      
        1 cggatgactg gcgatcttcc ggcagataaa ccagaccgcg cagtaaacgt tctccagtgg
       61 ataatttatt gatctcttta ccattcagca taatgcgtcc gccacgcaaa gtacgcagac
      121 catagagcgt ctcggccagt tctgtgcgtc cggcccccac cagcccggcc agacccagaa
      181 tttctccggc attgagcgtc aggctgacat tcctgaaacc ttcgccggtc agattttcta
      241 gtgtcagcac cgacgttccg gcggcatgtt gtgggcggtt gccaggtaac tccagccata
      301 atttttggct ggcagagagc gatttttccc gtaccactgg cgtgatggcc tgaataatgt
      361 catcggtaga cagttcgctg gttttgccgc ttaaggcgat ggttccgtcg cgcatcacgc
      421 taattcgatc ggcaatctgg cgaatttccg gcagcttatg cgagataaaa acaataccca
      481 cgccagtagc aagcagctct tgcaagcgac gaaacaagcg ttcggtttcc gcaggggtaa
      541 gcgagg
//
LOCUS       DAJVXP010000380          540 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVXP010000380 DAJVXP010000000
VERSION     DAJVXP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.387"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..141
                     /locus_tag="OQS10_004376"
     CDS             <1..141
                     /locus_tag="OQS10_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295206.1"
                     /translation="RRDRPDIAVRYYYKGVLWSVGYFASSCGGAPISVIRQYIEQQQT
                     PG"
BASE COUNT          181 a          121 c           88 g          150 t
ORIGIN      
        1 cgacgagatc gaccagatat tgcagtcagg tattactaca aaggcgtttt gtggagtgtt
       61 ggctattttg ccagtagctg cggaggtgcg ccaatatccg tcatccgcca atacattgaa
      121 caacagcaaa cacctggtta ggtggaaaac cacgccttat atccctgccc tgaaggacgg
      181 ggttttacgg cgcaccggat aagcctggag attatctaca ccagcatgga catcattgta
      241 ctatgccccg tacatgcccc atcacatcct caacatcaaa gtattagagc cttgcaaacc
      301 attatgcgtc tcagtttgtc tcatctatct tacgcaaaaa aaaatagaac taatatcaag
      361 cggcgctata ttgcgtatta aataaaacca cctcaattac attaataaat ctaaacaaac
      421 atcatgcaaa taatttccat ggttatttct aggctattta aattaaataa ataaattatg
      481 agtcaatcat aaataacaac ctttaatata atttaactac caaatttata ttcgttcaca
//
LOCUS       DAJVXP010000381          537 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVXP010000381 DAJVXP010000000
VERSION     DAJVXP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.388"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            129..464
                     /gene="iraM"
                     /locus_tag="OQS10_004377"
     CDS             129..464
                     /gene="iraM"
                     /locus_tag="OQS10_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS2295207.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          202 a          108 c           82 g          145 t
ORIGIN      
        1 taaaccaccc tgcatttaaa taaacaataa acaacatcag aaaaataatt ctgctctatg
       61 gtttacattc aaaaatatca tttatacttt tcagaacatc accagcaagg cataaacaag
      121 gaaactaaat gaagtggatt gtgattgata cagttatcca gccatcatgc ggaatatctt
      181 tttcagtcat atggagtaaa ataaaattaa taatctggta tcaatcggat gctttcttac
      241 ctcctgaaag tatatttaca ctgactcaca caggcatcat gctcaataac aaagtgctac
      301 ctgtaaccat ttacaacgta gtaccattca ataaaacatt ctggaattca atcaaaaata
      361 gccaggaatg ccctacaaat acagataacg tattgaatga atgctttaat aaccgttgca
      421 ctctgcaaat atgtccttat ggactaaaac aacaaagtcc ataaggagtt tactcacatc
      481 tgacaaaatc aatataaaca gcccctccgg agaggggctg gagagtggcg ctatgtg
//
LOCUS       DAJVXP010000382          534 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVXP010000382 DAJVXP010000000
VERSION     DAJVXP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.389"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>534)
                     /gene="dgcZ"
                     /locus_tag="OQS10_004378"
     CDS             complement(<1..>534)
                     /gene="dgcZ"
                     /locus_tag="OQS10_004378"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCS2295208.1"
                     /translation="SMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPY
                     VRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRS
                     NMDVLTGLPGRRVLDESFDHQLRNTDPLNLYLMLLDIDRFKLVNDTYGHLIGDVVLRT
                     LATYLASWTRDYETVYRY"
BASE COUNT          165 a          131 c          107 g          131 t
ORIGIN      
        1 gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg ccagggtgcg
       61 taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa atcggtcaat
      121 atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat gatcaaagga
      181 ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc tacggatcgt
      241 cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc cctcctgaaa
      301 ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta attcccgacc
      361 acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta attcatcgtt
      421 atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat aagaatggtt
      481 atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca tact
//
LOCUS       DAJVXP010000383          527 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVXP010000383 DAJVXP010000000
VERSION     DAJVXP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.390"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            10..228
                     /locus_tag="OQS10_004379"
     CDS             10..228
                     /locus_tag="OQS10_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS2295209.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            230..>527
                     /locus_tag="OQS10_004380"
     CDS             230..>527
                     /locus_tag="OQS10_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS2295210.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMG"
BASE COUNT          160 a          104 c          125 g          138 t
ORIGIN      
        1 cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag aacaacagaa
       61 ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct aatttttgtc
      121 tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt ggtatgggcg
      181 tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata tggcactgac
      241 gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt attgcggctg
      301 cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt ttgatggaaa
      361 tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc acccatgcaa
      421 tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg atggtcgtcg
      481 tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggat
//
LOCUS       DAJVXP010000384          520 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVXP010000384 DAJVXP010000000
VERSION     DAJVXP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.391"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>520
                     /locus_tag="OQS10_004381"
     CDS             <1..>520
                     /locus_tag="OQS10_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS2295211.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJVXP010000385          517 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVXP010000385 DAJVXP010000000
VERSION     DAJVXP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.392"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            356..>517
                     /locus_tag="OQS10_004382"
     CDS             356..>517
                     /locus_tag="OQS10_004382"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS2295212.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT          197 a           98 c           76 g          146 t
ORIGIN      
        1 gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt aattatgatc
       61 gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc atttcgtaat
      121 tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac tctatgtgaa
      181 ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg agaaaaagat
      241 atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac ttgcaacatt
      301 gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat ttattatgtt
      361 cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc
      421 ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt
      481 aacccgcgat gaaaagctta tcgcgtgcgg tggaatt
//
LOCUS       DAJVXP010000386          517 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVXP010000386 DAJVXP010000000
VERSION     DAJVXP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.393"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..413)
                     /locus_tag="OQS10_004383"
     CDS             complement(<1..413)
                     /locus_tag="OQS10_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS2295213.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
BASE COUNT          125 a          149 c          122 g          121 t
ORIGIN      
        1 tatttggcca ggcccaggcg agggatctca atagccgggc agcgatccat caccacattc
       61 aacccggcat cccgtgccag tactgccgct tgctcattaa tcacccccag ttgcatccag
      121 agggttttcg cgccaatggc aatagcttcc tgtgccacgc cccacgccgc ttctgaatta
      181 cggaaaacat cgaccatatc gaccttttcc ggaacgtctg ccagcgtgcc atacccctgc
      241 tggcccagca gcgttttgcc ggcaactttt ggcgaaaccg gaatgacgtg atacccctgg
      301 tcgagtagat atttcatcac gcgatagctg ggacgatcgg gtttgtcgct tgcccccacc
      361 agcgcaatgg tgtgagtgga cgttaaaatg cctgcaatat cggtttcttt cattatttcc
      421 tccagactgt ttaacaaagt gtacgacaaa gccgacatgc caaccatgca gctaatacga
      481 atgaatgaga gcaaaagcgg gaaatatgtc tatatgt
//
LOCUS       DAJVXP010000387          514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVXP010000387 DAJVXP010000000
VERSION     DAJVXP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.394"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..219
                     /locus_tag="OQS10_004384"
     CDS             <1..219
                     /locus_tag="OQS10_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS2295214.1"
                     /translation="PAEDKVTVKRAIASASRQRVLVCDATKYGQVATWLALPLSEFDQ
                     IITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(270..>514)
                     /locus_tag="OQS10_004385"
     CDS             complement(270..>514)
                     /locus_tag="OQS10_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCS2295215.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
BASE COUNT          141 a          113 c          122 g          138 t
ORIGIN      
        1 ccagcggaag ataaagtcac ggtgaaacgg gcgattgcca gtgccagccg ccagcgagtt
       61 ttggtctgtg atgcgacgaa atatggtcag gtggcgacat ggctggcgtt accattaagc
      121 gagtttgatc agattatcac agacgacggt ttgccggaga gtgccagtcg cgcgctggcg
      181 aagcaggatc tctctttgct ggtagcgaaa aatgaataat ggcctgcaat aacatttggt
      241 tactcatgct tcacagaaga agcatgagac tactttattt tataaaatga cagccgcccg
      301 cttttcggcg tgccggtatc aatataaatc tggttagcga acgtctgaat gttatcaaac
      361 atcatatgtc caaatataaa ataatcagcg ccgtttattt gttgtaactc gccattaagc
      421 gatttctgca cacgatcaac aggccagagt aattcgcttt ctgctatttc tttaccaaac
      481 tgatattcat cacccggata atctgcatgt gcga
//
LOCUS       DAJVXP010000388          507 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVXP010000388 DAJVXP010000000
VERSION     DAJVXP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.395"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            73..455
                     /locus_tag="OQS10_004386"
                     /pseudo
     CDS             73..455
                     /locus_tag="OQS10_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          135 a          120 c          128 g          124 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaa
//
LOCUS       DAJVXP010000389          504 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVXP010000389 DAJVXP010000000
VERSION     DAJVXP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.396"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>504
                     /gene="fliD"
                     /locus_tag="OQS10_004387"
     CDS             <1..>504
                     /gene="fliD"
                     /locus_tag="OQS10_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS2295216.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFT"
BASE COUNT          178 a          111 c          112 g          103 t
ORIGIN      
        1 ataaagaagt agacccaggt gctgaagcac aagataaaaa taatggtgca cttctcggag
       61 acagcgttgt acgtacaatt caaagcggca tccgcgcaca atttgcaaac ggtgccagcg
      121 acagtgcctt caaaacatta aacgagatcg ggattaagca ggacggcacg acgggtaagc
      181 tgaaaattga tgatgataaa ctcaaaaaag tcctgaatga aaatacagca tccgtacgtg
      241 aattgctggt aggcgatggt aaagaaacag gaattaccac caaaattgcc accgaggtaa
      301 aaggttacct tgccgatgat ggcattattg atagcgcaca ggacagtatt aacgccacac
      361 tgaaaaaact gaccaaacag tatctatccg tcagtgccag cattgatgac accgtggcgc
      421 gttacactgc acagtttacg caattagata ccatgatgag caaactgaat aacaccagta
      481 cctatctcag ccaacaattt actg
//
LOCUS       DAJVXP010000390          499 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVXP010000390 DAJVXP010000000
VERSION     DAJVXP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.397"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..362)
                     /locus_tag="OQS10_004388"
     CDS             complement(<1..362)
                     /locus_tag="OQS10_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCS2295217.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLR"
BASE COUNT          151 a          132 c          111 g          105 t
ORIGIN      
        1 cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg ccgggttcgc catatccact
       61 ttgcttttca tatcgacaga gagcacaggt tcaggcaacg cctccagtaa cgcgctcaac
      121 gccagatgct cacgttcgga aggcatccac gggacagtac gcacatcggt aacacccgca
      181 atacggcgta tttcggccat cagactgctg aaactctcaa actccagttc agcaaaattg
      241 aggtagattc gcccaatggg atcaatctca ataccgcgta aatcaatgcc tcttagcacg
      301 agtagatcga gtaattcgcg ggtcagaccg agtcggtctt cacaaaagac ttccagacgc
      361 atgggaacct tcacctgaaa aaagaacaat taatatgatg atatgacact atttggatag
      421 caggaaggtt tctgtcaaca atcattgaca caaagacgga aaaacgggca cttcggcgta
      481 aagatatccg gtcagcacc
//
LOCUS       DAJVXP010000391          491 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVXP010000391 DAJVXP010000000
VERSION     DAJVXP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.398"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>491
                     /locus_tag="OQS10_004389"
     CDS             <1..>491
                     /locus_tag="OQS10_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS2295218.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKI"
BASE COUNT          169 a          106 c          107 g          109 t
ORIGIN      
        1 taaagtttcc accgaagcag gtgctgcccc cggtatctat accatcagcg taacccaact
       61 ggcgcaggcg caatctttac gaactgattc cccgacaatc attgcatcaa caaaagatgc
      121 actgggtgat gagtcatcag atacacgcac aataaaaatt acccaagacg gtcgtaaaga
      181 gccgctggaa atcaagctca ataaagatca gacgtcattg gatgagatca gcaaagccat
      241 taacgatgcc gatagtggaa tttccgctag tatcgttaaa gtcaaagatg gtaactatca
      301 attggtattg accgccagtg aaggtctggc caataaaatg accatatctg ttgagggtga
      361 cagtaaatta aatgatctgc tggcttatga cagcaagacc aacaccggga atatgaagga
      421 actggttaac gcgcaaaatg cacagctaaa cgttaacggt atcgatattg aacgttcaag
      481 taacaaaatt a
//
LOCUS       DAJVXP010000392          489 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVXP010000392 DAJVXP010000000
VERSION     DAJVXP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.399"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OQS10_004390"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OQS10_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS2295219.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..>489)
                     /gene="fliA"
                     /locus_tag="OQS10_004391"
     CDS             complement(367..>489)
                     /gene="fliA"
                     /locus_tag="OQS10_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this sigma factor directs
                     late flagellar biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS2295220.1"
                     /translation="YYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKLGKL"
BASE COUNT          125 a          120 c          103 g          141 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaata
//
LOCUS       DAJVXP010000393          483 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVXP010000393 DAJVXP010000000
VERSION     DAJVXP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.400"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          137 a          109 c          105 g          132 t
ORIGIN      
        1 atcatcatca gcgtcgatat ggacgaatat aaaaacattt acgttatatc ccaaaaacac
       61 acaggtctta gggaggttta aactgtgtat taacacctat cgtattgacg ggcgtgaaat
      121 ggcagaaaca gaagtcgttc ctattgacat gcctgactct aacggcgaga tgacttggca
      181 agccaaaaac tacacacagt attcgtccta ctttatgaag attacttgct tgaaatagct
      241 tattggcaaa tggcatatac agttattgaa ccattctgat cttcggttgt atcgcacgca
      301 gccgaccatg agttattggc tgatgggaat gacctggtaa gccatatgta accagttcgt
      361 gaggtgcata ttccgccgcc acctgtaaca cgttttccag ttggacaggt agctgttgct
      421 ccaggaaagc gccaacctgt cgcctgtgct gtagcaattt gaagctcacc agatcctacc
      481 ttt
//
LOCUS       DAJVXP010000394          482 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVXP010000394 DAJVXP010000000
VERSION     DAJVXP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.401"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..224
                     /locus_tag="OQS10_004392"
     CDS             <1..224
                     /locus_tag="OQS10_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295221.1"
                     /translation="SYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNR
                     RLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            389..>482
                     /locus_tag="OQS10_004393"
     CDS             389..>482
                     /locus_tag="OQS10_004393"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCS2295222.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTC"
BASE COUNT          144 a          107 c          110 g          121 t
ORIGIN      
        1 actcgtatga caacgcgatg gcagagatca tcaagggcct ttacaaagcg gaggtaatac
       61 accgtaagag ctggaaaaac cggacagaag tggagctggc cacactcacg tgggtggact
      121 ggtataacaa tcgacgattg ctggaaaggc tgggccatat cccaccggca gaagcagaaa
      181 aagcttatta tgcttccatc ggaaatgatg atctggcagc ctgagttcac agataaaaca
      241 ctctccagga aacccggggc ggttcagttc acaaaaacac attaatgcag taactatatt
      301 ttcctttctg gtgataacgt cgggttatca ttagcttctt cagctatttt acttttatat
      361 cccttgtaaa catcatggag aatccatagt gaataaagtt tattctctta aatattgccc
      421 cgtcaccggg gggcttattg ctgtctctga acttgcccgc agggtaataa aaaagacatg
      481 cc
//
LOCUS       DAJVXP010000395          476 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVXP010000395 DAJVXP010000000
VERSION     DAJVXP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.402"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>476
                     /locus_tag="OQS10_004394"
     rRNA            <1..>476
                     /locus_tag="OQS10_004394"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJVXP010000396          474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVXP010000396 DAJVXP010000000
VERSION     DAJVXP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.403"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(215..>474)
                     /gene="dgcZ"
                     /locus_tag="OQS10_004395"
     CDS             complement(215..>474)
                     /gene="dgcZ"
                     /locus_tag="OQS10_004395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCS2295223.1"
                     /translation="IIVKAANDEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSR
                     AFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT          122 a          115 c           98 g          139 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atga
//
LOCUS       DAJVXP010000397          468 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVXP010000397 DAJVXP010000000
VERSION     DAJVXP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.404"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(78..425)
                     /locus_tag="OQS10_004396"
     CDS             complement(78..425)
                     /locus_tag="OQS10_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCS2295224.1"
                     /translation="MTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          140 a          109 c          123 g           96 t
ORIGIN      
        1 tcatatctcc aggtaaaaaa ggcccctccc aacacatggg acaaaatgaa aggaggagcc
       61 tcggaaaata cttttaatta atgtgcgtta gacgcggtgt ggttatccat tgagtggctc
      121 tggcgctcgt gcattttgcg agcgtagaac atcaggataa tcaggctgac gatgaaggta
      181 cctgccagct cgaaggagct tgcgcccatc agcgcgatga agcagaacac acaacccagt
      241 acagagcaga tcaggctgac aaagttgcgg atgttaacgc cttcaaaacg aatcaggtca
      301 acgcaagagt agaagtacgg cagcatagtc agcagtactg cgataccggt cagttcaccg
      361 aacaggtcag atgctttacc accggcagag ttcatcagag tgatgaggat tatcagggca
      421 gtcattttca ctgcagccag cagcagtaat gccgatcagt taaggatc
//
LOCUS       DAJVXP010000398          467 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVXP010000398 DAJVXP010000000
VERSION     DAJVXP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.405"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..467)
                     /locus_tag="OQS10_004397"
                     /pseudo
     CDS             complement(1..467)
                     /locus_tag="OQS10_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT           77 a          150 c          142 g           98 t
ORIGIN      
        1 acggcggttc aggctgtcgg tgatggtgat gcggtccttc tcatactgat acgtgtagct
       61 taagcctgcc gggttaagct gttctgtcac ccgcccgtcg ctgtcgtaac ggtaacggat
      121 ttccggccgg cccgtgtgac ggtgcgccac catccggccc cggtatttat catcgtaagt
      181 aaagctgcgc acctgtttgc cactacggtc atacaccacc gccagttcgc cgcgtggcgt
      241 ccagccatag cgcaccagcg gcgcggcagg taaattctcc gggtattccg ggtcgtgcgt
      301 cagccacacg gcagacagat gccgttgtcc cggccatatt cggtgtaacc cggcagggta
      361 tcaggaaaag cggacggttc cgtcccgccg gaaatggcct gctgccgggc ttcttctgcc
      421 cgctgcgcct gcgtggtcag taccagccgg aagtgacgcc cggcacc
//
LOCUS       DAJVXP010000399          465 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVXP010000399 DAJVXP010000000
VERSION     DAJVXP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.406"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..310)
                     /locus_tag="OQS10_004398"
     CDS             complement(<1..310)
                     /locus_tag="OQS10_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS2295225.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     E"
BASE COUNT          153 a           67 c          101 g          144 t
ORIGIN      
        1 attccagcga tgctggcaat tctggtaatg atattagggc attctgagta atttccagaa
       61 cagtgatttg aggtggtaag ttgtcaggta gcgaggacag atttaaacga ttcagttgaa
      121 gctcactgaa ctgattgatg agacattctt taagtaggga gaccgcttca ttccgatttt
      181 caccggggag cgcttgtttt tcccatttat cccatgctga aaagtaatca gcatatgtac
      241 cggaaatagt gttataaaaa gaattttggg acaatgaaaa gttattattt atcggtaaca
      301 tgatggacat tcagttaaag ccgatggaca aatggctttg ttaaaagaaa taagtaggag
      361 tttttatgaa gttcattcca tgaaatgaac ttcagcactt ttttattata aaaggacaaa
      421 caaaattggc tgtaaaaaaa cgatactgtt tcagttaact gtgaa
//
LOCUS       DAJVXP010000400          463 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVXP010000400 DAJVXP010000000
VERSION     DAJVXP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.407"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(85..>463)
                     /locus_tag="OQS10_004399"
     CDS             complement(85..>463)
                     /locus_tag="OQS10_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS2295226.1"
                     /translation="NTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEV
                     VPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMA
                     FENIKQVIDDMATDATMFDVNKK"
BASE COUNT          106 a          124 c          104 g          129 t
ORIGIN      
        1 ccgcgcctta tatccccgcc ctgaaggatg gggttttacg gcgcaccgga taaaaagcgc
       61 catctaacga tggcgcgtct ggaactactt tttgttaacg tcaaacatgg tggcatcggt
      121 cgccatgtca tcaataactt gtttaatatt ttcgaaagcc attggcgtac tttcgttatt
      181 caggtcttta ccttcgccct gacgaacgac tttgataacc ggtttattag tcgctgcatc
      241 aatcagctca ccttcaaaat agagtcgagt atccatggta cggtggcctg tagccatttg
      301 cgtccccgca accactaatg caacaggaac cacttcatag aattgcagcc cttctttgct
      361 ggtatctaca ccggtaatgg ccccacggaa aatcagacta cgcggcccag cggtggtaac
      421 cagtggttta cgctgcgcta tcgcttcttt catttcggtg ttg
//
LOCUS       DAJVXP010000401          461 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVXP010000401 DAJVXP010000000
VERSION     DAJVXP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.408"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>461
                     /locus_tag="OQS10_004400"
     CDS             <1..>461
                     /locus_tag="OQS10_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295227.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAJVXP010000402          459 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVXP010000402 DAJVXP010000000
VERSION     DAJVXP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.409"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            45..302
                     /locus_tag="OQS10_004401"
     CDS             45..302
                     /locus_tag="OQS10_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295228.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            302..>459
                     /locus_tag="OQS10_004402"
     CDS             302..>459
                     /locus_tag="OQS10_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295229.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSG"
BASE COUNT          135 a           98 c          124 g          102 t
ORIGIN      
        1 tacagtaata aacgaactga cagcagtaag atggagcgtt tcttttgaac aaccagacaa
       61 tgacttttac ccctgaacaa ttgcgtaaac acgcgcagga aatgctgcgt catgctgaac
      121 aactcgaaaa aacgggcata acaaaagacg ctatccgtaa agatatggta cccgcgcttc
      181 gggaactgat gcaggcgaag catcgcgcac agaaagcagt agatgagctg gtggactgtg
      241 tggcagagct ggaaaccaga gtcggaaagt ttgaaaaact ggtgcaggag gtgctgcgct
      301 gatgcgccat gagtttattt taccttatcc gccgacggtg aatacttact ggcgacgtcg
      361 tggcagcaca tattttgtat caaaagttgg tgagcgttat cgccgtgatg tgacgctaat
      421 tgttcgccag cagcggctga aattaaacct gtccggaag
//
LOCUS       DAJVXP010000403          456 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVXP010000403 DAJVXP010000000
VERSION     DAJVXP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.410"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(65..456)
                     /locus_tag="OQS10_004403"
                     /pseudo
     CDS             complement(65..456)
                     /locus_tag="OQS10_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          151 a           53 c           86 g          166 t
ORIGIN      
        1 taaataacat gagctgcata gtagggcaat tgttcttccc agaaacattt aaaaaattta
       61 agtttttgct ctgttttatt atgggaaata aagttccaat atcggatgta ttattagtat
      121 caatgtgggt tattttattg ttgtccagtc ttaaactttt aatagatgga agacaagtca
      181 ggaaatcaag atttatattt ttcaactggt tgttagcagc gtttaatgtc tgaacattgc
      241 agaagttgct atagaatatt ttaatttcag aaagcaggtt tttgttaata agtaacgtat
      301 tcaagttggg aaataagaca ttgtgtaaac ttgttagttt attatgggat agatccagca
      361 gtaagagtta atttttatta cctgcaacaa tatcagataa ttgattatgg ctgagattca
      421 ggtattgcag acattcgcaa gattctaatt gcacaa
//
LOCUS       DAJVXP010000404          452 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVXP010000404 DAJVXP010000000
VERSION     DAJVXP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.411"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>452)
                     /locus_tag="OQS10_004404"
     CDS             complement(<1..>452)
                     /locus_tag="OQS10_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCS2295230.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPL"
BASE COUNT           75 a          145 c          128 g          104 t
ORIGIN      
        1 caacggctgt ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt
       61 ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca
      241 gccgtccatc tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta
      301 gtcatacact tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctggccag ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat
      421 ttgtgcgctg tagcgaaact ggctgatgca ga
//
LOCUS       DAJVXP010000405          451 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVXP010000405 DAJVXP010000000
VERSION     DAJVXP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.412"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          129 a          105 c           87 g          130 t
ORIGIN      
        1 cgctcctaaa attcaattca caacgcagac ctacaacata gctaaaaaca ctcgtaattt
       61 aaggctcggg gtccatgcat attgctcatg gacttacctg aatggctcac cctttggtgg
      121 ttttcaacag gtatattccg accaaaacaa cgtttggtat gtgagtaatt atgcttgggg
      181 aaattatgag tctggtggga ccatatcagt cacatgcctc aatcttcctg gtgctggagt
      241 ttaattaaag gggcaacagt aggcatcgac atgttcctca tcaacaccat caccaatacc
      301 gtacatgcgt gttccagtca taatgtatcc agtcggacat tgaatcggct tgtaatagat
      361 cgaaccacca ctcttaccgc cgataaagtg attcattgct acggatgatt tgtaccaatt
      421 acatgctgag taattaattt tattaccccc t
//
LOCUS       DAJVXP010000406          443 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVXP010000406 DAJVXP010000000
VERSION     DAJVXP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.413"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            220..>443
                     /locus_tag="OQS10_004405"
     CDS             220..>443
                     /locus_tag="OQS10_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295231.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIR"
BASE COUNT          129 a           93 c          108 g          113 t
ORIGIN      
        1 tgatctcatg cagatgtttt gtgaatgtgt gggttgacat tcatatgaaa aaaatcataa
       61 ttccatcatg tttgtgtggg gtcttttctg taccttacgc atcgcactca agcctgacag
      121 aaaatatgct gtaaggctca tatcaaaaac cgccattagc tcatcaggaa gagcagacga
      181 caaccaggat tgttgtatgg tacgggggtc gaggcctcga tggcggtcca acttttcaaa
      241 actcttctga atcagatccc cttattatca tccctgcaaa gtggaacatt gccattgttt
      301 ggctattcag gacgggtcag accaatgaaa aaagcccaca caggggagag tgggctgaaa
      361 tgggaagcta aagactcaag taaacttatc ggaaataagg accacgcatt acgggggcta
      421 tcatcgccga tagtcgggat tcg
//
LOCUS       DAJVXP010000407          437 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVXP010000407 DAJVXP010000000
VERSION     DAJVXP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.414"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>437)
                     /locus_tag="OQS10_004406"
     CDS             complement(<1..>437)
                     /locus_tag="OQS10_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS2295232.1"
                     /translation="FKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNF
                     CFGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGA
                     DEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVG"
BASE COUNT           94 a          120 c          117 g          106 t
ORIGIN      
        1 aaccgactaa tgtccccata ttgatgttta ccgccgaagt gggatcggcc atctgacgga
       61 cgttggtgtc agtaccggag cgggtatctt tctgactttg ttcggttagc cagcttaacg
      121 cctcttcatc cgcgcccgct ttgtagtgtt cccatttggc gcgagcggca tcgtcggttt
      181 catcggcaat caccataaac aacacataag agccaacgtc gcgcccggtt tgctctgcgg
      241 cctgtttcat ccgcacagcg gtcggggcga aagccgtggg tgtatttacg cctttgccga
      301 aacagaagtt gaaatcggca tactgagcgg agaacgccat gccagcgtcg ctttgcccgg
      361 cgcagatcac tttcatgggg acactcggtt gcggactgac gcgacaatca ttcatggtga
      421 aaaaatcgcc tttaaaa
//
LOCUS       DAJVXP010000408          436 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVXP010000408 DAJVXP010000000
VERSION     DAJVXP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.415"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>436
                     /locus_tag="OQS10_004407"
     CDS             <1..>436
                     /locus_tag="OQS10_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS2295233.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAJVXP010000409          436 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVXP010000409 DAJVXP010000000
VERSION     DAJVXP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.416"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>436
                     /locus_tag="OQS10_004408"
     CDS             <1..>436
                     /locus_tag="OQS10_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS2295234.1"
                     /translation="YSAIAQHPHYNTIRTTGRAEATIQADIAHQFWHIPHEKIWIEDQ
                     STNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYP
                     GFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPRLRDDS"
BASE COUNT          114 a          120 c          105 g           97 t
ORIGIN      
        1 gtatagcgcc atcgcacagc atccgcacta caacactatc cgcaccactg gcagagcaga
       61 agcgaccatc caggcggata tcgctcatca gttctggcac attccgcatg aaaaaatctg
      121 gattgaagac cagtcaacaa actgcggtga aaacgcacgc tttagcatcg cgctattgaa
      181 tcaggccgta gaacgagttc atacggctat cgttgttcag gaccccacca tgcagcggcg
      241 cacgatggcg acgttccgcc gtatgactgg ggacaatccc gatgcaccac gctggttaag
      301 ttatcccgga ttcgttcctc agttaggaaa taacgcagac agtgtaatct ttattaatca
      361 gttacaagga ttatggccgg ttgagcgtta tctctcacta ctcactggcg agctgccgcg
      421 tttacgcgat gatagc
//
LOCUS       DAJVXP010000410          433 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVXP010000410 DAJVXP010000000
VERSION     DAJVXP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.417"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            89..>433
                     /locus_tag="OQS10_004409"
     CDS             89..>433
                     /locus_tag="OQS10_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS2295235.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIG"
BASE COUNT          103 a          104 c          127 g           99 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt ggg
//
LOCUS       DAJVXP010000411          427 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVXP010000411 DAJVXP010000000
VERSION     DAJVXP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.418"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>427)
                     /locus_tag="OQS10_004410"
     CDS             complement(<1..>427)
                     /locus_tag="OQS10_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS2295236.1"
                     /translation="RAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASL
                     SSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYT
                     AGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT           69 a          156 c          132 g           70 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgca tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 caacgtgtcg gggaaggaag gagctgagag agggcaggga ctgtcagggg aagataacga
      301 agcggtgtgc ggtttacggg cctcttccgc ccgctgcgcc tgtgtggtca gcaccaggtg
      361 aaaacggcgg cccgcgccgt ccatcacccc cgtcaccgcg cccgccacgt caccctccgc
      421 tgcccgg
//
LOCUS       DAJVXP010000412          422 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVXP010000412 DAJVXP010000000
VERSION     DAJVXP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.419"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..256
                     /locus_tag="OQS10_004411"
     CDS             <1..256
                     /locus_tag="OQS10_004411"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS2295237.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT          113 a          103 c           99 g          107 t
ORIGIN      
        1 gaccgaagaa gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc
       61 gtatccgccg ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc
      121 ggttctggag ttcctgcaga tgctgtgtga aatcggcact cactatccgg gatttgaaac
      181 cgatattcac ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga
      241 agaaagcaaa aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct
      301 cataaggaga acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga
      361 tccaaatctg ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca
      421 cc
//
LOCUS       DAJVXP010000413          421 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVXP010000413 DAJVXP010000000
VERSION     DAJVXP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.420"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            115..222
                     /locus_tag="OQS10_004412"
     CDS             115..222
                     /locus_tag="OQS10_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS2295238.1"
                     /translation="MTLAELGMAFWHDLAALVIAGILASMIVNWLNKRK"
BASE COUNT          115 a          104 c          119 g           83 t
ORIGIN      
        1 agaaaacccc cgcacgttgc aggtatgcac ctgacaacat cacgggggcc attcttgaac
       61 ctgaacaact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg ctatatgacg
      121 ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctctggtcat tgctggcatt
      181 cttgccagta tgatcgtgaa ctggctgaac aagcggaagt aacgtgtcat gcgggcgtca
      241 ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact ctgcggcctt
      301 tttcgtaaga aaaatgcaac gattgcacgc ggataatatt ctgcggaatg cgcagcacag
      361 agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg gcaagccgga
      421 a
//
LOCUS       DAJVXP010000414          420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVXP010000414 DAJVXP010000000
VERSION     DAJVXP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.421"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..272
                     /locus_tag="OQS10_004413"
                     /pseudo
     CDS             1..272
                     /locus_tag="OQS10_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          148 a           71 c           72 g          129 t
ORIGIN      
        1 ttcgcctagt gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca
       61 ctatcctatt aaaaatagca tataccccat ataatataca cacttacaag acagaggaag
      121 ttgtaaacca gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt
      181 actgtaataa cgattattac aatcaggcaa taagagagga accgatagac cttctggaca
      241 gatcgttttc ctccagttca tggccttttt agtgaataaa tcatcactaa ttctataagt
      301 gtctggtctt gttgataata tagttaatat gtaacccttt agattcacga tctgaagggt
      361 tgaaacatga catacgttca aagattaacc ccgtttgaag gttagcttgt tagttacatt
//
LOCUS       DAJVXP010000415          418 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVXP010000415 DAJVXP010000000
VERSION     DAJVXP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.422"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>418
                     /locus_tag="OQS10_004414"
     CDS             <1..>418
                     /locus_tag="OQS10_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2295239.1"
                     /translation="ATGSRKTMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHREE
                     GLSQYRAYDSRGQLIAVKDTQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIC
                     TTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT          100 a          119 c          128 g           71 t
ORIGIN      
        1 gccaccggca gccggaaaac catgacatgg agccgttacg gtcagttgct gaccgtcact
       61 gactgttccg gttatgtaac ccgttatgac catgaccgtt ttggtcaggt gacggcggtg
      121 caccgcgagg aagggctgag ccagtaccgc gcatacgaca gccgtggaca gttaattgcg
      181 gtgaaagaca cgcagggcca tgaaatgcgg tatgaataca acgccgccgg tgacctgacc
      241 gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagcc
      301 atctgtacca cgcagggcgg gctgacgcgc agtatggaat acgatgctgc cggacgggtc
      361 atccgtctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacc
//
LOCUS       DAJVXP010000416          417 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVXP010000416 DAJVXP010000000
VERSION     DAJVXP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.423"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            30..206
                     /locus_tag="OQS10_004415"
     CDS             30..206
                     /locus_tag="OQS10_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295240.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWCKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          149 a           46 c          101 g          121 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtgtaaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcagg
//
LOCUS       DAJVXP010000417          416 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVXP010000417 DAJVXP010000000
VERSION     DAJVXP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.424"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..256)
                     /locus_tag="OQS10_004416"
     CDS             complement(<1..256)
                     /locus_tag="OQS10_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS2295241.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQ"
BASE COUNT           96 a           99 c           85 g          136 t
ORIGIN      
        1 cctgttgcag ctcgctgggc atactttcca gccagctcag ccagtctctg gcctgggaaa
       61 gtagatgctc gccagccgct gtcaacgtca cgctgcgagt cgtgcggaaa aacagcgcca
      121 ctccggtatt ctcttccaga agtttaatgc gataactgat cgtcgccgtg gttttacata
      181 atcgttctgc cgcttttgaa aaacttcctg tttcagcaac cgcaatgaaa gtccgcaagg
      241 tttctggatc gaacatcttc aggtatcccc ttttaaatcc gcaagttgcg tgattttctt
      301 atcctctgat ttatcagtat ttttacatga taaccctgtt caatttgtgg actaaatcta
      361 gttttggaaa aatattccaa cttttgtatt gatgttgttc tcttaaggtt ttagat
//
LOCUS       DAJVXP010000418          413 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVXP010000418 DAJVXP010000000
VERSION     DAJVXP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.425"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          119 a           87 c          108 g           99 t
ORIGIN      
        1 tattcgattt gccgttgggg ttaacaaaca aaaaccccgg attgatggtc cggggttttt
       61 gaaggaaaca aaacagaaac agcaattgcc gttacctgtt gttaccatgg caagtaaacg
      121 tatctcaggc gagcgcattg cgccgttctg acgcagataa actagcctgg atagatggtg
      181 ctggcaataa aaaatagcgt tttcttatcg gtgtcggtaa gattgctgcg ggtgcttgag
      241 gctgtctgcc tcgggtatgt cactgtaagg cagacagaga aaagccccag ttaacattac
      301 gcgtcctgca agacgcttaa cattaatctg aggcccaatc tatgtctcac aaatgtaggt
      361 tagcctctta cgtgccgaaa ggcaaggaga agcaggctat gaagcagcaa aag
//
LOCUS       DAJVXP010000419          413 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVXP010000419 DAJVXP010000000
VERSION     DAJVXP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.426"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            1..413
                     /locus_tag="OQS10_004417"
                     /pseudo
     CDS             1..413
                     /locus_tag="OQS10_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          123 a           93 c          107 g           90 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac gga
//
LOCUS       DAJVXP010000420          408 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVXP010000420 DAJVXP010000000
VERSION     DAJVXP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.427"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            54..130
                     /locus_tag="OQS10_004418"
     tRNA            54..130
                     /locus_tag="OQS10_004418"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OQS10_004419"
     tRNA            173..248
                     /locus_tag="OQS10_004419"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAJVXP010000421          406 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVXP010000421 DAJVXP010000000
VERSION     DAJVXP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.428"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(4..>406)
                     /locus_tag="OQS10_004420"
     CDS             complement(4..>406)
                     /locus_tag="OQS10_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS2295242.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           85 a          110 c          136 g           75 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttct
//
LOCUS       DAJVXP010000422          403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVXP010000422 DAJVXP010000000
VERSION     DAJVXP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.429"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>403
                     /locus_tag="OQS10_004421"
     CDS             <1..>403
                     /locus_tag="OQS10_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS2295243.1"
                     /translation="RSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAF"
BASE COUNT          115 a           88 c           89 g          111 t
ORIGIN      
        1 ccgttccgat attgcctatc agatttatcg tgatttcgcc gaaaacaaag ggctttttgt
       61 acctggtgcc aatgatattc cggtatatga taaggacgga aaacttgtgg gaagactggg
      121 taaagcccca atggccgatt tcagcagtgt gagctcaaat ggcgttgcta cgcttgtatc
      181 gcctcagtat atcgtcagcg taaagcataa cggaggatat cggagtgtga gctttggtaa
      241 tgggaaaaat acatattccc ttgttgaccg taataaccac ccttctattg acttccatgc
      301 tccacgtctg aataaactgg ttacagaagt tattccctca gcggtaacat cagaaggaac
      361 caaagccaat gcttataaat acactgaacg ttacaccgct ttt
//
LOCUS       DAJVXP010000423          391 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVXP010000423 DAJVXP010000000
VERSION     DAJVXP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.430"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>391
                     /locus_tag="OQS10_004422"
     CDS             <1..>391
                     /locus_tag="OQS10_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS2295244.1"
                     /translation="WSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYD
                     SRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT           93 a          112 c          118 g           68 t
ORIGIN      
        1 tggagccgtt acggtcagtt gctgagcttc accgactgtt ccggttatgt aacccgttat
       61 gaccatgacc gcttcgggca gatgacggcg gtgcaccgcg aggaagggct gagtcagtac
      121 cgcgcatacg acagccgtgg acagttaatt gccgtgaaag acacgcaggg ccatgaaacg
      181 cggtatgaat acaacgccgc cggtgacctg accaccgtca ttgccccgga cggcagcaga
      241 aacgggacac agtacgatgc gtggggaaaa gctatctgta ccacgcaggg cggtctgacg
      301 cgcagtatgg aatacgatgc tgccggacgg gtcatccgcc tgaccagtga aaacggcagc
      361 cacaccacct tccgttacga tgtactcgac c
//
LOCUS       DAJVXP010000424          389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVXP010000424 DAJVXP010000000
VERSION     DAJVXP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.431"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>389)
                     /locus_tag="OQS10_004423"
     CDS             complement(<1..>389)
                     /locus_tag="OQS10_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS2295245.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           71 a          134 c          114 g           70 t
ORIGIN      
        1 cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc caccagcccg gtcagtaccc
       61 ggtacggcgg cagcggcgca ggcagcacct catccgcttc cggcacccgc tcacaccagc
      121 cgagcagcca ccacggcccc tgcggactgt ttgtcgccag ataacgatgc ggacttaagc
      181 ggagttcttc cggcagcgcc tgccagagtg cggccagccg gtgaccttca tccagtttcg
      241 ccacgccgcc gcgcaccagc cagagtgatt cgctgcggct gtaaccgtcc tcaccgggaa
      301 acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt gtgttatcgc
      361 gcagctgtaa gcggatatcc gcaggcatt
//
LOCUS       DAJVXP010000425          388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVXP010000425 DAJVXP010000000
VERSION     DAJVXP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.432"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..363)
                     /locus_tag="OQS10_004424"
     CDS             complement(<1..363)
                     /locus_tag="OQS10_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCS2295246.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          102 a          116 c           89 g           81 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaa
//
LOCUS       DAJVXP010000426          388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVXP010000426 DAJVXP010000000
VERSION     DAJVXP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.433"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>388
                     /locus_tag="OQS10_004425"
     CDS             <1..>388
                     /locus_tag="OQS10_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295247.1"
                     /translation="SMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILI
                     TLHYKKEIIPLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQP
                     TVNLNGSAFFSQSPSDAIFCRHLSLQY"
BASE COUNT          129 a           87 c           52 g          120 t
ORIGIN      
        1 tcgatgctgt catactgtgt tactcaggaa tttaccgcaa actgtcgaaa aatttcacaa
       61 aattgttatt ccactgccct ttttatcatt aactttgcca cttcacccat ccatgcagaa
      121 aatatactca ttacattaca ctataaaaaa gaaatcattc ccttatttct ggaaaccacg
      181 cctattaagg ctaaccattt gcgaagcata ctggattata ttgaacagga acagttaact
      241 gccgaagatc gtaaccattg tatgaaactg tctaaaaaaa tccatagaga aaaaactata
      301 caaccaacag taaatctcaa tggtagtgca tttttttcgc aatctccttc tgacgctatt
      361 ttttgtcgcc atctgtcatt gcaatacg
//
LOCUS       DAJVXP010000427          386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJVXP010000427 DAJVXP010000000
VERSION     DAJVXP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.434"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..382)
                     /locus_tag="OQS10_004426"
     CDS             complement(<1..382)
                     /locus_tag="OQS10_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCS2295248.1"
                     /translation="MDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRA
                     GVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQST
                     PQMSTLQQPISASWYVFFRRGLITD"
BASE COUNT          115 a          103 c           93 g           75 t
ORIGIN      
        1 gatcggtaat taatccgcgg cgaaaaaaga cataccagga ggcgctaatc ggttgttgta
       61 gtgtgctcat ttgcggtgtt gactggcggc gcatgctgca ccacgcaaac cataagagat
      121 aagcgccgcc gacgattctg ataagcgaaa aaatctcctc acactgcgtt attagcgttg
      181 caagaccaaa caaacccaac ccggaataaa atgcatcgcc cagcgccacg cccagtccgg
      241 tcagcacccc tgcgcgtcga ccggaagcca ggctggtttg tactaccaca aagagattgg
      301 ctcccggatt aaaaaaagta atcacgaaca gtcctacggt caggtaaacg gcgtgcaaag
      361 gatccatagt gatttcatcc ataaat
//
LOCUS       DAJVXP010000428          384 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJVXP010000428 DAJVXP010000000
VERSION     DAJVXP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.435"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>384
                     /locus_tag="OQS10_004427"
     CDS             <1..>384
                     /locus_tag="OQS10_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS2295249.1"
                     /translation="KHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNL
                     LLIGPPGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHH
                     SASLTAMVGGGAIPGPGEISLAHNG"
BASE COUNT          101 a           95 c          110 g           78 t
ORIGIN      
        1 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
       61 atctcagtga tgttgtcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
      121 gcaggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
      181 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
      241 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
      301 ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
      361 aaatttcgtt ggcgcataac ggcg
//
LOCUS       DAJVXP010000429          384 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJVXP010000429 DAJVXP010000000
VERSION     DAJVXP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.436"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>384
                     /locus_tag="OQS10_004428"
     CDS             <1..>384
                     /locus_tag="OQS10_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295250.1"
                     /translation="YSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRF
                     VGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVI
                     PLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           80 a           97 c          102 g          105 t
ORIGIN      
        1 tgtattccgt ctcctcggtg ctgattaacg tcttcgtcag cagcaaagag ttgaaattta
       61 ccggcggatc ggtgcaggta ttccacggca tggcggcgta ttttggtcta ccggaagcgt
      121 tgatgaatcg ctttgtcggt ctggtgtcct ttaccgcgat gttcggttcc ctgctgatgt
      181 ggaccgcaac gccggtgaaa attttcttct ccgaaatccc ggaaggcatc tttggtaaga
      241 aaaccgtcga actgaacgaa aacggcgttc cggcgcgcgc agcgtggatc cagttcctga
      301 tcgtcatccc gctgatgatt atcccgatgc tcggttccaa taccgtgcag gatctgatga
      361 atactattat taatatgacc gccg
//
LOCUS       DAJVXP010000430          383 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJVXP010000430 DAJVXP010000000
VERSION     DAJVXP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.437"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>383
                     /locus_tag="OQS10_004429"
     CDS             <1..>383
                     /locus_tag="OQS10_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2295251.1"
                     /translation="SDNSRQVSLSTDGGFVLHSGGLTFSNDSFSDSDTLAVVQAPGAQ
                     GARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVELKSTSAVAVPRQGSVV
                     FADFETVQGQSAIMNITRSDGKNIP"
BASE COUNT          102 a           84 c           95 g          102 t
ORIGIN      
        1 tagcgataac agtcgtcaag tgtctctcag caccgacggt ggttttgtat tgcatagcgg
       61 cggcctgacc ttcagtaatg acagttttag cgactccgat acactggcgg tagttcaggc
      121 tccaggtgct caaggagcgc gaataaatta tggcaacagt actatcgatc gatggggtta
      181 tggtgtcacc agcgctcttt ctccttatca tgaaaaccgt atcgcgctgg atatcaacga
      241 tcttgagaac gatgttgaat taaaaagtac cagtgcagta gctgtaccgc gtcagggttc
      301 agtcgtcttt gctgattttg aaaccgtgca agggcaatca gccattatga acatcacacg
      361 aagtgatggt aaaaatattc cat
//
LOCUS       DAJVXP010000431          368 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJVXP010000431 DAJVXP010000000
VERSION     DAJVXP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.438"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>368
                     /locus_tag="OQS10_004430"
     CDS             <1..>368
                     /locus_tag="OQS10_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS2295252.1"
                     /translation="RLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQ
                     VWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPA
                     GLIHHSDRGSQYCAYDYRVI"
BASE COUNT           96 a          100 c           98 g           74 t
ORIGIN      
        1 cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag agcgactacg
       61 aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt cgctcctaca
      121 gcaccaaatc aggtctgggt ggcggacctg acgtatgttg ccacacagga gggatggttg
      181 tacctcgctg gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc catgggagag
      241 cgcatgacaa aagagctgac aggtaaagcc ctgtttatgg cgctcaggag ccagcgccca
      301 cctgccgggc taatccacca ctctgatcga ggttcacagt actgcgcata cgattaccgg
      361 gtcataca
//
LOCUS       DAJVXP010000432          367 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJVXP010000432 DAJVXP010000000
VERSION     DAJVXP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.439"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          147 a           55 c           45 g          120 t
ORIGIN      
        1 tacgaatcgc actctatcaa ttttcactta aagtatgaac tggatatctt tattcataag
       61 aactattcat caacagcgta tagaggcggt tatgtaaaac cactcgttag cctcaaaaca
      121 aagacaaatt tccagattta ttacttaatc aatgaataac aagcttaaaa aacaaactat
      181 taacctaaag aaaaaattaa cagacattaa ttcaatatta aaaaaataat agattaaaat
      241 ttcttaacga tttaagaatc atacaaataa cactttgatt aaatttaatt ttttgtatcg
      301 ataattgtga acctctctgg catggagaac tattttgaac atgaggtgtt acgtggatat
      361 tttgctt
//
LOCUS       DAJVXP010000433          365 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJVXP010000433 DAJVXP010000000
VERSION     DAJVXP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.440"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..332)
                     /locus_tag="OQS10_004431"
     CDS             complement(<1..332)
                     /locus_tag="OQS10_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS2295253.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVR"
BASE COUNT           99 a           92 c          107 g           67 t
ORIGIN      
        1 cggacagcct cacttcccag gcgctggcgg gcctgaataa cggcactggg ggcaacgaag
       61 gggcgattgc ccggcagcat gatgtccagg cgattcacaa tctggtgaag aggttcttta
      121 cgctcaagcg ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg
      181 cgtagcgtta cagtacctga ttcggcaagg caacgagaga tgagttcggg gtcgaggtaa
      241 tcccccagag aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc
      301 tgtccaatgt gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc
      361 actgg
//
LOCUS       DAJVXP010000434          363 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJVXP010000434 DAJVXP010000000
VERSION     DAJVXP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.441"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..359)
                     /locus_tag="OQS10_004432"
     CDS             complement(<1..359)
                     /locus_tag="OQS10_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295254.1"
                     /translation="MLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVISG
                     PRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPLA
                     GFLILAAWRVFVGDPSG"
BASE COUNT          142 a           93 c           64 g           64 t
ORIGIN      
        1 aaaccggagg ggtcacccac aaaaactcgc caggcggcta atatcagaaa cccagccaaa
       61 ggtataaata acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga
      121 ataacccaat ataaccaccg cggaaaatgt acggatataa gcatacaata ttctttcaat
      181 gccagaaaac ttatcagcgc aaaaaacgcc aacgtcatcc agcgtggacc tgaaatcacc
      241 agtgaaaaac aaatgacgat gccccaccag gtacgaatac gtaatgtaac ctcaccccct
      301 tttctctccg gtaaccgcca tagcagaaaa cggtttatca gcgttgctaa aattaacaac
      361 gcg
//
LOCUS       DAJVXP010000435          363 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJVXP010000435 DAJVXP010000000
VERSION     DAJVXP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.442"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            225..>363
                     /locus_tag="OQS10_004433"
     CDS             225..>363
                     /locus_tag="OQS10_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS2295255.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          108 a           65 c           64 g          126 t
ORIGIN      
        1 acattttaat ataacgagtt gcatttagtt acatgtttaa cacttgattt aagatttgta
       61 atggctagat tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga
      121 tttattacta cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag
      181 gcagctgtca ggtgtgcgat caataaaaaa agcggggttt catcatgttt aatgaagtcc
      241 atagtattca tggtcataca ttattgttga tcactaaacc ttctttgcag gcgacggctc
      301 tcttgcagca ccttaaacaa tcgctggcaa ttacaggaaa attacataat attcaacgtt
      361 ctc
//
LOCUS       DAJVXP010000436          360 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJVXP010000436 DAJVXP010000000
VERSION     DAJVXP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.443"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>360)
                     /gene="adeQ"
                     /locus_tag="OQS10_004434"
                     /gene_synonym="yicO"
     CDS             complement(<1..>360)
                     /gene="adeQ"
                     /locus_tag="OQS10_004434"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS2295256.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTL"
BASE COUNT          136 a           84 c           65 g           75 t
ORIGIN      
        1 taatgttcct gatgaatcaa ataggttgat cagcataaag gagaaaatga taccggcgag
       61 ttcaagtgtt aacgcgccgc tcaaatctac ttcaccaatg acgccgctaa tatcaggcgg
      121 aatggaatag acgccgctaa aatgaacatc accgaaaaat aatccacagc aagacgtcac
      181 cacaatagaa accagcaccg cggcatgaaa atgacgtgat gacaacacgg ttataataaa
      241 aaaacctaaa atacctaaca acacgccgtg agaacttaaa tcgccaatca tcaccagcgt
      301 gtctttattg gcgacaataa cgccagtatt ttttaatccc attaaggcga taaataatcc
//
LOCUS       DAJVXP010000437          355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJVXP010000437 DAJVXP010000000
VERSION     DAJVXP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.444"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          118 a           62 c           74 g          101 t
ORIGIN      
        1 gtgtagtcct ttcgtcatgt aaaaagttgt tgttttttct ccacacctcc gtggacaaat
       61 ttttactcca aaaagacgag gtttgtcacg gcttgtgcgc aagacatatc gcagcaatca
      121 gcgatgggca agaagaatga ctgtctggtg atttttgata gcggaaacgg agatttaaaa
      181 gaaaacaaaa tattttttgc gtagataaca gcgtatttac gtggctttta atactttggt
      241 atgaacaaaa aaagaaaaat acaacgcgcg ggtgagttat taaaaatatt tccgcagaca
      301 tactttccat cgtaacgcag cgttaacaaa atacaggttg cgttaacaac caagt
//
LOCUS       DAJVXP010000438          353 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJVXP010000438 DAJVXP010000000
VERSION     DAJVXP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.446"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..249
                     /locus_tag="OQS10_004435"
     CDS             <1..249
                     /locus_tag="OQS10_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HCS2295257.1"
                     /translation="LALRDKKRFDGEIFDLDISLDRVEGAALEFYEAAARRSIRQVFL
                     DVAAGLCEGDELLPETRPCSEARYTIKINSSDNSITGC"
BASE COUNT           85 a           75 c           97 g           96 t
ORIGIN      
        1 ttagcgttac gggacaaaaa acgctttgac ggtgaaattt ttgacctgga catctcgctg
       61 gaccgtgttg aaggtgccgc gctggagttt tatgaggcag cagccagaag gagcatcaga
      121 caggtcttcc tggatgttgc tgccgggtta tgtgaagggg acgagctgtt gccagaaacg
      181 cgcccctgtt cagaggcgcg gtataccata aaaattaaca gttctgataa ctcgattacg
      241 ggttgttagc tttttgcagt tggctttcca gtatctttca ttggtagcat cctgataaat
      301 atccatgagc gcaaaaatca aatacggcct gtcagctgct gttctggcgc tga
//
LOCUS       DAJVXP010000439          351 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJVXP010000439 DAJVXP010000000
VERSION     DAJVXP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.447"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>351
                     /locus_tag="OQS10_004436"
     CDS             <1..>351
                     /locus_tag="OQS10_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCS2295258.1"
                     /translation="GNEGTIKTADDLKGKKVGVGLGTNYEEWLRQNVQGVDVRTYDDD
                     PTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEAFSRQESGVALRKGNEDLLK
                     AVNDAIAEMQKDGT"
BASE COUNT          100 a           73 c          107 g           71 t
ORIGIN      
        1 aaggtaacga aggcaccatt aaaacagccg atgatctgaa aggcaaaaaa gtgggtgtcg
       61 gtctgggcac caactatgaa gagtggctgc ggcagaatgt tcagggtgtc gatgtgcgta
      121 cctatgatga tgacccgacc aaatatcagg atctgcgcgt aggccgtatc gatgcgatcc
      181 tcgttgaccg tctggcggcg ctggatctgg tgaagaaaac caacgatacg ctggcagtaa
      241 ccggtgaagc attttcccgt caggagtctg gcgtggcgct gcgtaaagga aatgaagatc
      301 tgctgaaagc agtgaatgat gcaattgcgg aaatgcaaaa agatggcact c
//
LOCUS       DAJVXP010000440          348 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJVXP010000440 DAJVXP010000000
VERSION     DAJVXP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.448"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>348
                     /locus_tag="OQS10_004437"
     CDS             <1..>348
                     /locus_tag="OQS10_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS2295259.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYA"
BASE COUNT          102 a           71 c           83 g           92 t
ORIGIN      
        1 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
       61 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
      121 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
      181 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
      241 gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa tactggctat
      301 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgcc
//
LOCUS       DAJVXP010000441          346 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJVXP010000441 DAJVXP010000000
VERSION     DAJVXP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.449"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            38..250
                     /locus_tag="OQS10_004438"
     CDS             38..250
                     /locus_tag="OQS10_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295260.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
BASE COUNT          129 a           54 c           78 g           85 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctca
//
LOCUS       DAJVXP010000442          342 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJVXP010000442 DAJVXP010000000
VERSION     DAJVXP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.450"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>342)
                     /locus_tag="OQS10_004439"
     CDS             complement(<1..>342)
                     /locus_tag="OQS10_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2295261.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVP"
BASE COUNT          113 a           92 c           68 g           69 t
ORIGIN      
        1 ttcggtacat agccaatcat ccctaacaca accgcaatga aaaaagccgc aaaggctgaa
       61 ccgcccttca ccaccatagt ctgcacggag taagcgatac tttcacagcg tacgtgcagt
      121 ttatattcac cgtaatcaac ggtatctgcc accatgatga cctgtaatac ccagaaaagc
      181 gccgttccca cattcagcaa aatacccgca atcacaatga ggacgacgtt gtgatagctc
      241 attaatgcca ttaacaggag aacaccacag cttaacaccg gaagaataga tgctccggcc
      301 cataaaatgc gtcgggataa tgatttaact aagcgtggga ag
//
LOCUS       DAJVXP010000443          337 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJVXP010000443 DAJVXP010000000
VERSION     DAJVXP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.451"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>337)
                     /locus_tag="OQS10_004440"
     CDS             complement(<1..>337)
                     /locus_tag="OQS10_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS2295262.1"
                     /translation="GGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEG
                     SHLTLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLS
                     YAGAANLVTL"
BASE COUNT          115 a           72 c           75 g           75 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccg
//
LOCUS       DAJVXP010000444          331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJVXP010000444 DAJVXP010000000
VERSION     DAJVXP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.452"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          101 a           74 c           64 g           92 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct g
//
LOCUS       DAJVXP010000445          331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJVXP010000445 DAJVXP010000000
VERSION     DAJVXP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.453"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT          115 a           59 c           47 g          110 t
ORIGIN      
        1 attgaccgcc atttaaatca agcatactaa atccaataag tgacaaatct ttaatttata
       61 gatttcatga atatttaaat attaactgaa cgagtaattc attgattcaa catctaatga
      121 ctatccaaca aataaatatg cgtgatatat aaagcccttt ctttagttta ttttcagaaa
      181 aagatacaca aaatttatat ttggcatttt cttgtctctc cgacagactg accgaattgt
      241 tatcaatgtt aacaaaaaaa gaacaattgg ttataaggag agagtatgcg tgtcatcacc
      301 ctggcgggta gtcctcgctt tccttctcgc t
//
LOCUS       DAJVXP010000446          331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJVXP010000446 DAJVXP010000000
VERSION     DAJVXP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.454"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(30..279)
                     /locus_tag="OQS10_004441"
                     /pseudo
     CDS             complement(30..279)
                     /locus_tag="OQS10_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          111 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta a
//
LOCUS       DAJVXP010000447          330 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJVXP010000447 DAJVXP010000000
VERSION     DAJVXP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.455"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(158..>330)
                     /locus_tag="OQS10_004442"
     CDS             complement(158..>330)
                     /locus_tag="OQS10_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS2295263.1"
                     /translation="PTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHA
                     DIPVGAVSDVKA"
BASE COUNT           92 a           76 c           79 g           83 t
ORIGIN      
        1 tggcttgata accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct
       61 tatccggtct acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca
      121 tatcgcatca ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg
      181 caccgaccgg aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca
      241 gatgaatctg gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa
      301 tcagcgttac catgaaaaac agagttggta
//
LOCUS       DAJVXP010000448          327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJVXP010000448 DAJVXP010000000
VERSION     DAJVXP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.456"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..164)
                     /locus_tag="OQS10_004443"
     CDS             complement(<1..164)
                     /locus_tag="OQS10_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCS2295264.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSA"
BASE COUNT          108 a           56 c           79 g           84 t
ORIGIN      
        1 gcgctaaata gcatccagac acagaaggca agaagtagac aactgactga tatccagagg
       61 tttcttcgag caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag
      121 tcgcgtaaaa gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc
      181 acacattggt aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa
      241 ttaaggatga attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg
      301 ggcagaatgg ggattaagta gccagat
//
LOCUS       DAJVXP010000449          327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJVXP010000449 DAJVXP010000000
VERSION     DAJVXP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.457"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(1..327)
                     /locus_tag="OQS10_004444"
                     /pseudo
     CDS             complement(1..327)
                     /locus_tag="OQS10_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           90 a           79 c           77 g           81 t
ORIGIN      
        1 ttgctgacta ttaccgctac gaaaatcgcc cgccgtcagg tcaaatttga aacgcccttc
       61 gcgttgtaaa atcggcactg ttgaatacgg aattgtgtaa ttctgccatc gcgctcgtcg
      121 atggtgacat ccagatcgcc atttgaagat gtcgggtgca aatcagtaat ttcaaaagca
      181 ccgggggaaa catagctttg atagataata aaaccatttt gccgaatcgt tagctgggcc
      241 gccgtacggg caatcccacg taccgttggg gcaaaccctt gctggctatc tggatacata
      301 ttatcggaag aataaagccg tacacca
//
LOCUS       DAJVXP010000450          324 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJVXP010000450 DAJVXP010000000
VERSION     DAJVXP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.458"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..183)
                     /locus_tag="OQS10_004445"
     CDS             complement(<1..183)
                     /locus_tag="OQS10_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCS2295265.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSII"
BASE COUNT          106 a           69 c           62 g           87 t
ORIGIN      
        1 aataatgcta ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa
       61 taaaaaacct tttcttttac gatttccgcg gcgaaaccaa aagctcatca gaatcaatgc
      121 tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat
      181 cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaaacccc tggcggggct
      241 attttaccgc gacaattcat tcagatcatc aatagtcagg gaaggaagta gcaacattag
      301 ctaactaatc aatgcgttga ttgt
//
LOCUS       DAJVXP010000451          318 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJVXP010000451 DAJVXP010000000
VERSION     DAJVXP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.459"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>318
                     /locus_tag="OQS10_004446"
     CDS             <1..>318
                     /locus_tag="OQS10_004446"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Plays a crucial role on both purine and pyrimidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HCS2295266.1"
                     /translation="FVATCGDRGIDNDFRDVNDWQFFKGAQKLGELGQPVLVHCENAL
                     ICDAPGEEAKREGRVTAHDYVASRPVFTEVEAIRRVLYLAKVAGCRLHVCHISSPESV
                     EEVT"
BASE COUNT           68 a           77 c          103 g           70 t
ORIGIN      
        1 ttcgttgcga cctgtggcga tcgcggtatc gacaacgact tccgtgatgt aaacgactgg
       61 cagttcttca aaggtgcgca gaagctgggc gaactggggc agccggtgtt ggtgcactgc
      121 gaaaacgcgc tgatctgtga cgcaccgggc gaagaagcga aacgtgaagg tcgcgtaact
      181 gcccatgact atgtggcttc gcgtccggta tttaccgaag tggaagcgat tcgccgcgtg
      241 ctgtatctgg cgaaagttgc cggttgccgt ctgcacgttt gccatatcag cagcccggaa
      301 agtgttgaag aagtgact
//
LOCUS       DAJVXP010000452          317 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJVXP010000452 DAJVXP010000000
VERSION     DAJVXP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.460"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>317
                     /locus_tag="OQS10_004447"
     CDS             <1..>317
                     /locus_tag="OQS10_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS2295267.1"
                     /translation="WQTQDWVALGGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERD
                     EARDLLHSHGTGKGRAFCEKLAQQAGIDQSHPALQRWYASIHETQNDREVVSLYAHLV
                     QPV"
BASE COUNT           67 a           80 c          104 g           66 t
ORIGIN      
        1 cctggcaaac ccaggactgg gtagcgctgg gtggatgtgc cgctggggca ttgattatcg
       61 gcgctggcgt aggttctgtg gtaacagagt ggcagtgctt atggtgcttg cgacagcgcg
      121 cccatgaacg cgacgaagcg cgtgatttat tgcatagcca cggcacgggc aaaggccgcg
      181 cattttgcga aaaactggcg cagcaggcgg gtattgatca gtcgcatccg gcgctgcaac
      241 gctggtatgc ctcaatccat gaaacgcaaa acgaccgtga agtggtcagc ctgtatgcgc
      301 atttggtcca gccagtt
//
LOCUS       DAJVXP010000453          315 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJVXP010000453 DAJVXP010000000
VERSION     DAJVXP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.461"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>315
                     /locus_tag="OQS10_004448"
     CDS             <1..>315
                     /locus_tag="OQS10_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS2295268.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SE"
BASE COUNT           87 a           71 c           88 g           69 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagt
//
LOCUS       DAJVXP010000454          314 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJVXP010000454 DAJVXP010000000
VERSION     DAJVXP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.462"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            34..>314
                     /locus_tag="OQS10_004449"
     CDS             34..>314
                     /locus_tag="OQS10_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS2295269.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT           96 a           66 c           72 g           80 t
ORIGIN      
        1 ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc aaaatctctt
       61 ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa ggatattact
      121 gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac cgcgttgccg
      181 aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca gttgatgacc
      241 aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc cccggcgcaa
      301 ctggttcaaa gcct
//
LOCUS       DAJVXP010000455          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJVXP010000455 DAJVXP010000000
VERSION     DAJVXP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.463"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            5..>308
                     /locus_tag="OQS10_004450"
     CDS             5..>308
                     /locus_tag="OQS10_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCS2295270.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRA"
BASE COUNT          124 a           57 c           56 g           71 t
ORIGIN      
        1 aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat atcgaaggag
       61 agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac taggtaaaat
      121 ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat acaaaaacta
      181 ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta tggcagactg
      241 gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata cttataaaat
      301 tcgggcaa
//
LOCUS       DAJVXP010000456          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJVXP010000456 DAJVXP010000000
VERSION     DAJVXP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.464"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..>308
                     /locus_tag="OQS10_004451"
     CDS             <1..>308
                     /locus_tag="OQS10_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS2295271.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJVXP010000457          308 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJVXP010000457 DAJVXP010000000
VERSION     DAJVXP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.465"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT           90 a           73 c           59 g           86 t
ORIGIN      
        1 tttaccataa ctcccgttgt acgtgccctc tcagacgctc tctgacgcaa aaaactcaaa
       61 aagacaagaa aaaacagggt aaacataaaa aatgcgtcag aatcgttttt agcgcgttgc
      121 ataactatgc acgaaagcga cccgcgatcg gttttgtgtg ttctgaatac cgaacgggtc
      181 aaaatgacac gttttctctc cctcaatgga gcgttctata cagagatttt tgttccccta
      241 tccattattc tgattaccgg gtttccaagc tgaaaacagg acttttctgc gaccagtttt
      301 tgcgaata
//
LOCUS       DAJVXP010000458          304 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJVXP010000458 DAJVXP010000000
VERSION     DAJVXP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.466"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>304)
                     /locus_tag="OQS10_004452"
     CDS             complement(<1..>304)
                     /locus_tag="OQS10_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295272.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKR"
BASE COUNT           69 a           84 c           79 g           72 t
ORIGIN      
        1 tacgcttcag tacagcatgc catggtcccc agagggagaa ccagtccgta aattcattct
       61 cttcacggct tctgaccatg gcttcggcag tgcggaggtc atttgctgtc actcccgaca
      121 cgccatagaa acgcatttcc ttcacggcag tggagagctg aagtttctct gcgagcatgg
      181 tctggaaggc caggtagact tctatctcat ccacaaaatg gagagttctg actttatccc
      241 gggcaatgtc ctccagaatt tcgaggcgga acatttccct gcccagggag agcagagcgc
      301 cggt
//
LOCUS       DAJVXP010000459          301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJVXP010000459 DAJVXP010000000
VERSION     DAJVXP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.467"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>301)
                     /locus_tag="OQS10_004453"
     CDS             complement(<1..>301)
                     /locus_tag="OQS10_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295273.1"
                     /translation="HLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNV
                     FKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQTNWSTTQ"
BASE COUNT           64 a           62 c           75 g          100 t
ORIGIN      
        1 attgagttgt tgaccaattt gtctgtggca ggtaatcact tgctgtcttt gtctccatcc
       61 ctggatactg gggatcatcc tgcggcatac aagtcagatt gggttttacc cacttcctgc
      121 aatacagtgt gtaatcgtaa gggcttaatg ctctgaagaa tgttttgaaa acattaggcg
      181 cactactggc ctggtcaata ggatatggca gtcttaattg tttcgctatg tctggcaact
      241 tacttggatc gggcgtaggc ggtttgctgg gtttcgctcc atatccgcca ttatttaagt
      301 g
//
LOCUS       DAJVXP010000460          301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJVXP010000460 DAJVXP010000000
VERSION     DAJVXP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.468"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT           99 a           48 c           68 g           86 t
ORIGIN      
        1 tggtttgaac gaaataaagc ggaattaggt tattggtagt ccgagacttt aagagaaatt
       61 agaaaaacag cttatcagac gctctcaatc gcattctaac gcaaaaaact atcttatttt
      121 agggtgggta ataccactgt acaggctacc cctgaaaaag actgtttagc tcgttacaga
      181 gcgattgagg aggtctggta aaaaggagat tttaaaaatg aacgaatatg aattacagat
      241 tttcattgat agcgacactg ctatgatggt gcaggctttc gctgattccg gtgtatcaat
      301 t
//
LOCUS       DAJVXP010000461          298 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJVXP010000461 DAJVXP010000000
VERSION     DAJVXP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.469"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..>298)
                     /locus_tag="OQS10_004454"
     CDS             complement(<1..>298)
                     /locus_tag="OQS10_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS2295274.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLK"
BASE COUNT           96 a           46 c           44 g          112 t
ORIGIN      
        1 attttaaaaa agtctccaga atcgaatcga tgaccggtgt aaggttaact tttgcatcat
       61 ctaaggaatc aataagaata tgagacaggt taactttgat gtcattaatt tcattgtcag
      121 tagctttctt attattaaga agataattat ttactgcgtt aaggtcatct atttctttca
      181 gtatcccttt ttcaccaagc aagtatgaaa tcgattcatt acatacataa ggcattaata
      241 gcagatgttc tttccttatt gctaataatg cattgttcaa tggtttttct aataaatc
//
LOCUS       DAJVXP010000462          295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJVXP010000462 DAJVXP010000000
VERSION     DAJVXP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.denovo.470"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
BASE COUNT           86 a           64 c           57 g           88 t
ORIGIN      
        1 tcgagttttt tagagaaatt ttcgggaata tttcaggtca atccatgcag acgcaagcaa
       61 ttcctgtatt gaataattcc gtagcaatta tgtaaaatca tctccggctg attttcattc
      121 aaactcgcgc tatcgaacat ccatcagcca gccgtggcac gttcttgcat acgacgtgct
      181 acggtttcct ttacgatttg gggttgcgac tttacctcta ttgataatgc attccggcag
      241 aacgttcaaa tatgaagtac gatgtttaac taaccgaaaa acaagaacaa tacgg
//
LOCUS       DAJVXP010000463         5514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.199, whole genome shotgun sequence.
ACCESSION   DAJVXP010000463 DAJVXP010000000
VERSION     DAJVXP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.199"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..247
                     /locus_tag="OQS10_004455"
     CDS             <1..247
                     /locus_tag="OQS10_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HCS2295275.1"
                     /translation="KNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKA
                     ANIIVKDEKMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            258..617
                     /gene="frdD"
                     /locus_tag="OQS10_004456"
     CDS             258..617
                     /gene="frdD"
                     /locus_tag="OQS10_004456"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS2295276.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            680..1813
                     /locus_tag="OQS10_004457"
     CDS             680..1813
                     /locus_tag="OQS10_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS2295277.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1902..2435
                     /gene="blc"
                     /locus_tag="OQS10_004458"
     CDS             1902..2435
                     /gene="blc"
                     /locus_tag="OQS10_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS2295278.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2432..2749)
                     /gene="sugE"
                     /locus_tag="OQS10_004459"
     CDS             complement(2432..2749)
                     /gene="sugE"
                     /locus_tag="OQS10_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS2295279.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2924..3070)
                     /gene="ecnB"
                     /locus_tag="OQS10_004460"
     CDS             complement(2924..3070)
                     /gene="ecnB"
                     /locus_tag="OQS10_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS2295280.1"
                     /translation="MVKKTIAAIFSVLVLSTVLNACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3181..3306)
                     /gene="ecnA"
                     /locus_tag="OQS10_004461"
     CDS             complement(3181..3306)
                     /gene="ecnA"
                     /locus_tag="OQS10_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS2295281.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3358..3924)
                     /gene="efp"
                     /locus_tag="OQS10_004462"
     CDS             complement(3358..3924)
                     /gene="efp"
                     /locus_tag="OQS10_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS2295282.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3966..4994
                     /gene="epmB"
                     /locus_tag="OQS10_004463"
     CDS             3966..4994
                     /gene="epmB"
                     /locus_tag="OQS10_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS2295283.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            5384..5479
                     /locus_tag="OQS10_004464"
                     /pseudo
     CDS             5384..5479
                     /locus_tag="OQS10_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1407 a         1367 c         1369 g         1371 t
ORIGIN      
        1 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
       61 gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact
      121 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
      181 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
      241 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
      301 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
      361 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
      421 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
      481 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc
      541 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
      601 gtatcgttac aatctaacgt atcgccaatg taaatccggc ccgcctatgg cgggccgttt
      661 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
      721 ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
      781 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
      841 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
      901 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
      961 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttactgc
     1021 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     1081 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     1141 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     1201 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     1261 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     1321 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     1381 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     1441 aacccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     1501 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     1561 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     1621 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     1681 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     1741 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     1801 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     1861 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     1921 ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg
     1981 gcgtgacagt agtgaataat ttcgacgcta aacgctatct cggtacctgg tatgagattg
     2041 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagtctgc
     2101 gtgatgacgg cggcctgaat gtcatcaata aaggctataa ccctgacaga gaaatgtggc
     2161 agcagagtga agggaaagcg tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt
     2221 cattctttgg tcctttctat ggcggttata acgtcattgc acttgatcgg gaataccgcc
     2281 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     2341 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     2401 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     2461 tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca
     2521 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     2581 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     2641 tcacagtaat aacacttggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg
     2701 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     2761 gagaattggg gccatccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     2821 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt gtgactatcg
     2881 caaaaaaaca aacggcacga cataactgtc gtaccgttat tgcttattgc tgcgctttcg
     2941 ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     3001 tgttgcaggc atttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     3061 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     3121 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     3181 ttagctggca gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg
     3241 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     3301 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     3361 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     3421 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     3481 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     3541 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     3601 cagcagccat ttagcgttgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     3661 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     3721 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     3781 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     3841 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     3901 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     3961 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     4021 ttgccgatgt tgtgaccgat cctggtgaac ttctgcgtct tttgaatata gacgcggacg
     4081 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     4141 ttatcgatcg catggagaaa ggcaatccgg acgatcctat tttgcgtcag gtacttacct
     4201 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     4261 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     4321 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     4381 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     4441 agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc
     4501 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     4561 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     4621 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     4681 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     4741 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     4801 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     4861 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     4921 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     4981 tacgccagca gtaactgatt aaacagggaa gttaaacatt agcctgatgc gctacgctta
     5041 tcaggcctac attgtgcatt gaaattgaat ggatttgcaa tgaactgtag gccggataag
     5101 gcgtttacgc cgcatccggc aagagcaaca aacgcaagta cagcaacctg tcattaaact
     5161 gggaaattta atctttgccc gttgcataac ctttaattat tcatatacat tgctgttgtt
     5221 actctttata tagccaacac caataattaa cctttctttt ctgtttatat agagtgagta
     5281 cggctattta tgttatttta acaactatta ctaccaagcc catttatctg gcttataaat
     5341 taaaattaat tatttaattg agaaatggtt agggagaacc tacatggcca taagcatcaa
     5401 gggtgttaat accggtgtta ttcgcaagag taataatttc attgcactgg cgctgaaaat
     5461 taaagaacct cgcaataggt aatgactcca acttattgat agtgttttat gttc
//
LOCUS       DAJVXP010000464         4541 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.213, whole genome shotgun sequence.
ACCESSION   DAJVXP010000464 DAJVXP010000000
VERSION     DAJVXP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.213"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..172
                     /locus_tag="OQS10_004465"
     CDS             <1..172
                     /locus_tag="OQS10_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCS2295284.1"
                     /translation="AHERTPQQERQKAAREAERGREAWTLGQGMKKPVAGCYGRLTRW
                     KGGGDVVYMALL"
     gene            complement(204..854)
                     /gene="tetR(A)"
                     /locus_tag="OQS10_004466"
     CDS             complement(204..854)
                     /gene="tetR(A)"
                     /locus_tag="OQS10_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS2295285.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            960..2159
                     /gene="tet(A)"
                     /locus_tag="OQS10_004467"
     CDS             960..2159
                     /gene="tet(A)"
                     /locus_tag="OQS10_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS2295286.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2126..2314)
                     /locus_tag="OQS10_004468"
                     /pseudo
     CDS             complement(2126..2314)
                     /locus_tag="OQS10_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2358..2747)
                     /locus_tag="OQS10_004469"
     CDS             complement(2358..2747)
                     /locus_tag="OQS10_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295287.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2965..3405
                     /locus_tag="OQS10_004470"
     CDS             2965..3405
                     /locus_tag="OQS10_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS2295288.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(4161..4310)
                     /locus_tag="OQS10_004471"
     CDS             complement(4161..4310)
                     /locus_tag="OQS10_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295289.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1022 a         1251 c         1253 g         1015 t
ORIGIN      
        1 ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg
       61 ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg
      121 tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt agtgagtggg
      181 ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc aacgttcctg
      241 acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct
      301 gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga
      361 gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc
      421 acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa
      481 aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc
      541 ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag
      601 ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc
      661 gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg
      721 aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc
      781 agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc
      841 tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat
      901 cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagacg
      961 tgaaacccaa cagacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca
     1021 tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg
     1081 tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt gatgcaattt gcctgcgcac
     1141 ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc ggtcttgctc gtctcgctgg
     1201 ccggcgctgc tgtcgactac gccatcatgg cgacggcgcc tttcctttgg gttctctata
     1261 tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg
     1321 ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg
     1381 ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg
     1441 ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt
     1501 tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg
     1561 cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca
     1621 tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct
     1681 ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac
     1741 tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca
     1801 tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat
     1861 ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc
     1921 aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg
     1981 cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg
     2041 cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct
     2101 gcctgccggc gctgcgtcgc gggctttgga gaaattcttc aaattcccgt tgcacatagc
     2161 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
     2221 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
     2281 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
     2341 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
     2401 gctagacatt gccagccagt gcccgtcact cccactctta cattgttcgc agtatctagg
     2461 tacatgttat tgcgtgcacc ttcatgatgt ataatgttat taatgacagc agaaagatcg
     2521 acagaattaa tattcttcgt acctgggaca gttatcgggt gtcggccagt gatgagacca
     2581 aaggatgcag tataagatga accactacta ttatcatggc caccattacc accaccgtta
     2641 aaactacctc ccgaacttcc agagccgcta tgaccatcat tgtaggggtc atttggtccc
     2701 atgtgaccaa aaccaccgac agtaccgcta tttaactctg tactcataaa attatcctct
     2761 ttgactttta aaaacagcaa aaactgtatt tataacaatg tgtttatata cagtattcga
     2821 aatactacgc ccgtcaaaac cacaccgtca acaggttttt tgtgtatttt tcacgcctca
     2881 cgggcagcct cagacctttt gtgttaaaac agtaatatgt tctgctgtta caatacggta
     2941 cacacaggag acactgtcac ttttatggca tggcacacag ttcaagagac actgaaatta
     3001 acaggaaagt cgcgctcgca attatatcgt gacatggcta aaggccttgt cagctatcgc
     3061 acaggaaaaa atgatcgccg ggagttcgag accagtgaac tgattcgggc ctatggcgag
     3121 ctgcgcactt ctgagacacc aaaaaggcac aaggagggac actccaaatc tgtcgttagc
     3181 caggcggaaa atgctcagct agacgtcatt cagcagaaac tggacgctct gcagcagact
     3241 gttaccctaa tgcttgaaga caagatggcg cgggaagcag aaaaccgcct gcgaaatgag
     3301 gagagggaac aactacaggc cgaaattagc cgtctggctg acgcgctggc acaggaaaaa
     3361 aagcggggat tctggtcgcg gctgtttcgc tccggttcag agtaacagtg caggtgactc
     3421 tgatttacca taacatctgt tatcagcacc gcctgagagg ccctcagggc gtttctgacg
     3481 ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg
     3541 tttttacggc atgcatgagt atgcgcacaa gtgcatagcg cggaaattca caccattcag
     3601 ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat
     3661 ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa acccgctgtc agcacgttca
     3721 ggaagggggt gatgtacaaa tggcacagag gatgaaagtg acgtatgagc tgaaggaatg
     3781 atgtaaaagt gatgtataag tgatgtaaaa gtgatgtaaa agtgatgtat gaactgaagg
     3841 agcgatgtaa ttctgtagtc aggaagaata acccgttcat gcaggcatga acgatacagt
     3901 gaggaacgct ttttttgtca cgatctcccc ttaacctgcg taaatcaccc gccatggttg
     3961 attatttttc aacctcaaaa tggtcataca cctaacgcag ccgcgatttt catcgcaact
     4021 gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac gagtaatacc cgggtattaa
     4081 aaatccactg taattacatg aaaataaaga aataaaaggt aataccggag tattaccacg
     4141 tacgcgggca agcgaaaaag ctataacgct tcccggccga caggcgaccc aaaatccaca
     4201 ggggcatgca ggttctcttc agtaacctgc gccattaaat caaaaaggtc atattcgccg
     4261 ggaagctgcg tatcagtcga atccagttgt tcatcatatt cagatggcat ggggtgatat
     4321 ccagtagctg agaggttgtt agcgggtttc ggggcgcagc cctgaaccag tcatgtagcg
     4381 ctagcggagt gtatactggc ttactatgtt gccacgattg cgaaaatcag tgaagtgctt
     4441 catgtggcag gagaaaaaag gcagcaccgg cgcgtcagca gaatatgcga tacaggagat
     4501 attccgcttc ctcgctcact gactcgctac gctcggtcgt t
//
LOCUS       DAJVXP010000465         3879 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.228, whole genome shotgun sequence.
ACCESSION   DAJVXP010000465 DAJVXP010000000
VERSION     DAJVXP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.228"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OQS10_004472"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OQS10_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS2295290.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="OQS10_004473"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="OQS10_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS2295291.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="OQS10_004474"
     CDS             2675..3511
                     /locus_tag="OQS10_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS2295292.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..>3879
                     /locus_tag="OQS10_004475"
     CDS             3817..>3879
                     /locus_tag="OQS10_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCS2295293.1"
                     /translation="MKTAALDLARERQAHEAGART"
BASE COUNT          834 a         1092 c         1072 g          881 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggacc
//
LOCUS       DAJVXP010000466         3878 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.229, whole genome shotgun sequence.
ACCESSION   DAJVXP010000466 DAJVXP010000000
VERSION     DAJVXP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.229"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OQS10_004476"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OQS10_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS2295294.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1916..2674
                     /locus_tag="OQS10_004477"
     CDS             1916..2674
                     /locus_tag="OQS10_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCS2295295.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2674..3510
                     /locus_tag="OQS10_004478"
     CDS             2674..3510
                     /locus_tag="OQS10_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS2295296.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3816..>3878
                     /locus_tag="OQS10_004479"
     CDS             3816..>3878
                     /locus_tag="OQS10_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCS2295297.1"
                     /translation="MKTAALDLARERQAHEAGART"
BASE COUNT          834 a         1092 c         1072 g          880 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt ttttggtgaa tcgcattctg actggttgcc
     1921 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
     1981 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
     2041 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
     2101 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
     2161 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
     2221 tgatcaatgt cagtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
     2281 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
     2341 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga
     2401 tatggttgtt tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct
     2461 tcaatgcacg ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt
     2521 ggcactcatg attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc
     2581 cttcgctgtc ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt
     2641 ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct
     2701 cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa
     2761 gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt
     2821 gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg
     2881 ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct
     2941 cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg
     3001 aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc
     3061 tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac
     3121 gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta
     3181 catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat
     3241 cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct
     3301 gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct
     3361 cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt
     3421 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     3481 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     3541 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     3601 tcccgcaaac gacccctgga agccccgtag cgacgcggag aggggcgaga caagccaagg
     3661 gcgcaggctc gatgcgcagc acgacatagc cggttctcgc aaggacgaga atttccctgc
     3721 ggtgcccctc aagtgtcaat gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc
     3781 cacgctagat ctatctcatc tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg
     3841 cccgcgagcg ccaggcgcac gaggccggcg cgcggacc
//
LOCUS       DAJVXP010000467         2560 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.265, whole genome shotgun sequence.
ACCESSION   DAJVXP010000467 DAJVXP010000000
VERSION     DAJVXP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.265"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..498)
                     /locus_tag="OQS10_004480"
     CDS             complement(<1..498)
                     /locus_tag="OQS10_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCS2295298.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILI"
     gene            759..1217
                     /gene="gpt"
                     /locus_tag="OQS10_004481"
     CDS             759..1217
                     /gene="gpt"
                     /locus_tag="OQS10_004481"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS2295299.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1309..2553
                     /gene="frsA"
                     /locus_tag="OQS10_004482"
     CDS             1309..2553
                     /gene="frsA"
                     /locus_tag="OQS10_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS2295300.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
BASE COUNT          604 a          648 c          667 g          641 t
ORIGIN      
        1 aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc
       61 ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag
      121 aaccgccagc gcagacgcca taccaatgcc gttatctgca cccagcgtgg tgccgcgcgc
      181 tttaacccat tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt
      241 gtcgttattt ttctgcggca ccatatcgag gtgggcctgt aagacgaccg atttacgatt
      301 ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac
      361 atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata
      421 ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa
      481 ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg
      541 tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtttgagaa
      601 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
      661 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
      721 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
      781 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
      841 aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc
      901 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
      961 agcgcgagct taaagtgcta aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     1021 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     1081 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     1141 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     1201 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     1261 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     1321 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     1381 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     1441 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgt
     1501 gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     1561 ttatacgata cggtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccatccag
     1621 gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgtcac
     1681 tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa aggagatgac
     1741 ctggccgagc aagcgcaggc tttgtcaaac cgtgcctatg aagaagccgc ccagcgtctg
     1801 ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt
     1861 ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat
     1921 gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg
     1981 atgctgacta ttgatatgcc gtcggtgggc ttttcatcaa aatggaagct cacccaggac
     2041 tccagcctgt tgcatcagca cgttttaaag gctctgccta acgtaccgtg ggtggatcac
     2101 actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatat
     2161 cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg
     2221 agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt
     2281 ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg agctgaatcg ctattcatta
     2341 aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa tgttatcagg ctactggaag
     2401 aacgatccgt tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt
     2461 aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa
     2521 atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
//
LOCUS       DAJVXP010000468         2139 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.273, whole genome shotgun sequence.
ACCESSION   DAJVXP010000468 DAJVXP010000000
VERSION     DAJVXP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.273"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            <1..407
                     /gene="dfrA"
                     /locus_tag="OQS10_004483"
     CDS             <1..407
                     /gene="dfrA"
                     /locus_tag="OQS10_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCS2295301.1"
                     /translation="SAKGEQLLFKAITYNQWLLVGRKTFESMGALPNRKYAVVTRSSF
                     TSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEGD
                     VYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            502..1026
                     /gene="sat2"
                     /locus_tag="OQS10_004484"
     CDS             502..1026
                     /gene="sat2"
                     /locus_tag="OQS10_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS2295302.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1095..1592
                     /locus_tag="OQS10_004485"
     CDS             1095..1592
                     /locus_tag="OQS10_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295303.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1653..2024
                     /locus_tag="OQS10_004486"
     CDS             1653..2024
                     /locus_tag="OQS10_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS2295304.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT          596 a          501 c          523 g          519 t
ORIGIN      
        1 ggagtgccaa aggtgaacag ctcctgttta aagctattac ctataaccaa tggctgttgg
       61 ttggacgcaa gacttttgaa tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa
      121 cacgttcaag ttttacatct gacaatgaga acgtattgat ctttccatca attaaagatg
      181 ctttaaccaa cctaaagaaa ataacggatc atgtcattgt ttcaggtggt ggggagatat
      241 acaaaagcct gatcgatcaa gtagatacac tacatatatc tacaatagac atcgagccgg
      301 aaggtgatgt ttactttcct gaaatcccca gcaattttag gccagttttt acccaagact
      361 tcgcctctaa cataaattat agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa
      421 cggattcgca aacctgtcac gccttttgta ccaaaagccg cgccaggttt gcgatccgct
      481 gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca
      541 ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa
      601 ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat
      661 gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag
      721 attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac
      781 acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta
      841 agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc tgcctgcaat
      901 ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta taaaactaga
      961 cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc acaggatgac
     1021 gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca ggagttagag
     1081 agctggggaa gactatgcgc gatctgttga aggtggttct aagcctcgta cttgcgatgg
     1141 catcggggca ggcacttgct gacctgccaa ttgttttagt ggatgaagct cgtcttccct
     1201 atgactactc cccatccaac tacgacattt ctccaagcaa ctacgacaac tccataagca
     1261 attacgacaa tagtccatca aattacgaca actctgagag caactacgat aatagttcat
     1321 ccaattacga caatagtcgc aacggaaatc gtaggcttat atatagcgca aatgggtctc
     1381 gcactttcgc cggctactac gtcattgcca acaatgggac aacgaacttc ttttccacat
     1441 ctggcaaaag gatgttctac accccaaaag gggggcgcgg cgtctatggc ggcaaagatg
     1501 ggagcttctg cggggcattg gtcgtcataa atggccaatt ttcgcttgcc ctgacagata
     1561 acggcctgaa gatcatgtat ctaagcaact agcctgctct ctaataaaat gttaggcctc
     1621 aacatctagt cgcaagctga ggggaaccac tagtgtcata cgaacctcca agagacggtt
     1681 acacaaacgg gtacattgtt gatgtcatgt atgacaatcg cccaagtaag tatccagctg
     1741 tgttcagaac gtacgtccga attccagact atgtcgaact agataacgcg gaggagctgg
     1801 ccgacgcaat cattcgagag gttgagttaa aggaggcatg gctcacaagg gtcttgggga
     1861 actcagccga tcctgacgct tctgtgggca agtgggaccg gcgcctagcg cagtgcggtg
     1921 gtattgatga gatgtcaagt ggggtgcagg caacatactt cagtccaaac ggtcagctgg
     1981 tcggcttagt ggcatacatt gatgccgaat ggatggatga atagctctga cgttacgatt
     2041 caggtgagac aaccagatgg gcattgcgta caagctcagt tgccaccgat gtggctacga
     2101 gtctgatctt ctttacctag gccaaggtat ggcgatgct
//
LOCUS       DAJVXP010000469         1450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate 603011
            SAMEA8900653-rid15856233.guided.301, whole genome shotgun sequence.
ACCESSION   DAJVXP010000469 DAJVXP010000000
VERSION     DAJVXP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900653
            Sequence Read Archive: ERR6005567
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478481.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478481.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 06:44:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,489
            CDSs (total)                      :: 4,405
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 124 of 245
            Pseudo Genes (incomplete)         :: 62 of 245
            Pseudo Genes (internal stop)      :: 83 of 245
            Pseudo Genes (multiple problems)  :: 24 of 245
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900653-rid15856233.guided.301"
                     /serovar="S. sonnei"
                     /isolate="603011"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Bangladesh"
                     /collection_date="2009-09-12"
     gene            complement(<1..241)
                     /locus_tag="OQS10_004487"
     CDS             complement(<1..241)
                     /locus_tag="OQS10_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HCS2295305.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLY"
     gene            573..1046
                     /locus_tag="OQS10_004488"
     CDS             573..1046
                     /locus_tag="OQS10_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS2295306.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1141..>1450
                     /locus_tag="OQS10_004489"
     CDS             1141..>1450
                     /locus_tag="OQS10_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS2295307.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     S"
BASE COUNT          430 a          287 c          320 g          413 t
ORIGIN      
        1 tgtacaaaaa agctagggca tttaaagcga ttttctgcgt gtttatggct acatgtctgc
       61 tgtttgctaa gctggataaa aacagcctga cctcttcact gcccatggtc tgaggatgac
      121 gttttttgtg aaacagaata aaacgcttaa tccagtgcag gtaagttttt tcagttttca
      181 gcgcataacc tttttgtcgc atatccgtgc gtatagaatt taaaaatgga ctgttagaca
      241 taaaacgctc cttgtcttgc aactgtctgc ctatacagcc tattctagct gggatttaaa
      301 aaagtgcctg ttttttacgc ctagagatgc ttgtttaccg gtagagtttt aatttaatgc
      361 taaataaatt aaaatgttat gagttctttg ggtgagataa tgtgcatcgt gcaagcagga
      421 tagacggcat gcacgatttg taataacaga gtgtcttgta tttttaaaga aagtctattt
      481 aatacaagtg attatattaa ttaacggtaa gcatcagcgg gtgacaaaac gagcatgctt
      541 actaataaaa tgttaacctc tgaggaagaa ttgtgaaact atcactaatg gtagctatat
      601 cgaagaatgg agttatcggg aatggccctg atattccatg gagtgccaaa ggtgaacagc
      661 tcctgtttaa agctattacc tataaccaat ggctgttggt tggacgcaag acttttgaat
      721 caatgggagc attacccaac cgaaagtatg cggtcgtaac acgttcaagt tttacatctg
      781 acaatgagaa cgtattgatc tttccatcaa ttaaagatgc tttaaccaac ctaaagaaaa
      841 taacggatca tgtcattgtt tcaggtggtg gggagatata caaaagcctg atcgatcaag
      901 tagatacact acatatatct acaatagaca tcgagccgga aggtgatgtt tactttcctg
      961 aaatccccag caattttagg ccagttttta cccaagactt cgcctctaac ataaattata
     1021 gttaccaaat ctggcaaaag ggttaacaag tggcagcaac ggattcgcaa acctgtcacg
     1081 ccttttgtac caaaagccgc gccaggtttg cgatccgctg tgccaggcgt taggcgtcat
     1141 atgaagattt cggtgatccc tgagcaggtg gcggaaacat tggatgctga gaaccatttc
     1201 attgttcgtg aagtgttcga tgtgcaccta tccgaccaag gctttgaact atctaccaga
     1261 agtgtgagcc cctaccggaa ggattacatc tcggatgatg actctgatga agactctgct
     1321 tgctatggcg cattcatcga ccaagagctt gtcgggaaga ttgaactcaa ctcaacatgg
     1381 aacgatctag cctctatcga acacattgtt gtgtcgcaca cgcaccgagg caaaggagtc
     1441 gcgcacagtc
//
